LOCUS       DBMDXG010000001        40552 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_372_length_40552_cov_2.392055, whole genome shotgun sequence.
ACCESSION   DBMDXG010000001 DBMDXG010000000
VERSION     DBMDXG010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 40552)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 40552)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40552
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_372_length_40552_cov_2.392055"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(7..3162)
                     /gene="ileS"
                     /locus_tag="ACZPD3_00005"
     CDS             complement(7..3162)
                     /gene="ileS"
                     /locus_tag="ACZPD3_00005"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR1414434.1"
                     /translation="MYQKVDTNLNFVDREKKVEEFWKENHIFEKSMENRKEGETYTFY
                     DGPPTANGKPHIGHVLTRVIKDMIPRYRTMKGYMVPRKAGWDTHGLPVELEVEKKLGL
                     DGKEQIEEYGMEPFIKQCKESVWKYKGMWEDFSSTVGFWADMEHPYVTYYDDYIESEW
                     WALKEIWNKKLLYKGFKIVPYCPRCGTPLSAQEVSQGYKTVKERSAVVRFKVVGEDAY
                     FLAWTTTPWTLPSNVALCVNPDETYCKVKAADGYTYYMAEALLDKVLGKLVKEEGEKA
                     YEVLETYKGTDLEYKAYEPLFACAGEAAAKQKKKAHFVTCDNYVTMSDGTGIVHIAPA
                     FGEDDSRIGRNYELPFVQFVDGQGNLTKETPYAGVFVKKADPMVLTDLDKEGKLFDAP
                     KFEHDYPHCWRCDTPLIYYARESWFIKMTAVKDDLIRNNNTINWIPESIGKGRFGDWL
                     ENVQDWGISRNRYWGTPLNIWQCECGHMHSIGSRQELFEMSGDERAKTVELHRPYIDE
                     ITLKCPECGGEMHRVPEVIDCWFDSGAMPFAQHHYPFENKELFEQQFPANFISEAVDQ
                     TRGWFYSLLAESTLLFNKAPYKNVIVLGHVQDENGQKMSKSKGNAVDPFDALNKYGAD
                     AIRWYFYINSAPWLPNRFHGKAVVEGQRKFMSTLWNTYAFFVLYADIDNFDPTKYELN
                     YDQLPVMDKWLLSRLNTTVQAVDNDLANYKIPEAARALQEFVDEMSNWYVRRSRERFW
                     AKGMEQDKINAYMTLYHALVTIAKTAAPMIPFMTEDIYQNLVRSVDKDAPESIHLCDF
                     PTVNEAWIDKDLEADMKELLEIVVLGRACRNTANIKNRQPIGTMYVKAEKKMSEFYTD
                     IIADELNVKEVKFADDVESFISYSFKPQLRTVGPKYGKLLGGIRQALTDINGTAAMNE
                     LRTNGVLKLDINGNDVELTEEDLLIETAQTEGYVSESDGETSVVLDTNLTPELIEEGF
                     VREIISKIQTMRKEAGFEVMDKIVVYAHGNDKIQDVMKTHEDEIKSEVLADEMVLGET
                     DGYVKEWNINKEAVTMGVKKL"
     assembly_gap    3240..3249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     misc_binding    complement(3319..3554)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3841..5076
                     /locus_tag="ACZPD3_00010"
     CDS             3841..5076
                     /locus_tag="ACZPD3_00010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1414435.1"
                     /translation="MFKTKFHKLMKRFLTGMLCAGILTSQPAVTYAAETSATPEAGHS
                     ETYSQASDIDSVKGWPTGPNIEGQSAVLMDAVTDTILYSKNAKDKLYPASITKIMTAL
                     LACEYLDMNDTITMSQEAAYGIEAGSSSIYAETGEVFTVEQALMALMLESANEMALAL
                     AEKTSGSVKKFVELMNQRAAQLGCKNTHFNNPNGLPDETHYTTAGDMMKIAKAAWYNP
                     RFRKFVTTQVYEIPPTNKQSETRYLLNHHKMMPGQSYAYDGVLGGKTGYTDAAGSTLV
                     TYAKRGNSILIAVVLNSTNGAFPDTTSLLDYGFDNFEKVDLNIDTDPVPAVFLPCEKH
                     LLKDWNNLCSFYYMRHVYVTVPTGTDVSQLVKKQKLLNNSVGPKRIKSKYYLDGHMVG
                     YGMQYEKEILSDFLLNASF"
     gene            complement(5047..6006)
                     /locus_tag="ACZPD3_00015"
     CDS             complement(5047..6006)
                     /locus_tag="ACZPD3_00015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR1414436.1"
                     /translation="MNYIVFDLEWNQNPSGKKTRNDRLPFEIIEIGAVKVNSKKEITD
                     HFHRLIKPQVYKWIHDSIHEVIHVDYKDLMKGVPFEQAVREFIDWCGEDWYFFTWGNQ
                     DVMELQRNMKFYGLLDLLPGPVTYYDVQKLYSISYDDGTHRCALEHAIDELKIEKSRG
                     FHRALADAWYTAKVLEKINNIIIINHPSLDVYQNPKKKKDEIHISYPDHDKYVSREFA
                     TRERIMKDREVTSTRCPVCHLPAKRKLRWFMNNPKVYYSISNCEEHGLIRGKIRIRKT
                     EDEKYFAVKTLRFTDTEEAEELRERKEVLRLRRKLKRSVEKEI"
     gene            complement(6067..7191)
                     /locus_tag="ACZPD3_00020"
     CDS             complement(6067..7191)
                     /locus_tag="ACZPD3_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1414437.1"
                     /translation="MEKSTVYFTDFRCPVGTSQLDKLKKLCVTAGIKDIDMDGKFVAI
                     KMHFGELGNLAFLRPNYAKAVADLCKEQGGLPFLTDCNTLYPGSRKNALEHLECANLN
                     GFNSISTGCQIIIGDGLRGTDEVEVPVVNGEYCQTALIGHAIMDADIFISLSHFKGHE
                     ATGFGGALKNIGMGCGSRAGKMKQHASGKPAVNEELCRGCRRCAKECGSDAITYPNKK
                     AVIDYDKCKGCGRCIGACGFDAVYNPNSSANELLDRKMAEYAQAVCHGRPHFHVALVQ
                     DISPNCDCHGENDAPILPDIGMFASFDPVALDQACADACLKATPIENSQLGEHLAKPD
                     WHCHHDHFKDSNPNIEWQATLDQAEKIGLGTRQYELKIVK"
     gene            complement(7282..7527)
                     /locus_tag="ACZPD3_00025"
     CDS             complement(7282..7527)
                     /locus_tag="ACZPD3_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1414438.1"
                     /translation="MEEYEVISIPVSDGTERDFAIMDTFEFEGQGYLAVSLIEGDEIQ
                     EGVYIYRYHNAEDGDVVVEQITLPAEYKKVTKFYEQM"
     gene            complement(7752..9434)
                     /locus_tag="ACZPD3_00030"
     CDS             complement(7752..9434)
                     /locus_tag="ACZPD3_00030"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR1414439.1"
                     /translation="MYQEEYKRWLAADLQDADLNPELSKIEGNDEEIKDRFAVALKFG
                     TAGLRGVLGAGTNRMNIYVVRQATQGLANWVKTQGGNQTVAISYDSRLKSDVFAKTAA
                     GVLAANDINVRIYDALMPVPALSFATRYYECNAGIMVTASHNPAKYNGYKAYGPDGCQ
                     MTDDAAAIVYEEIQKTDVLTGAKYMSFAEGVEQGKIRFVGDDCKQALYEAIESRQVRP
                     GLCKTAGLKLVYSPLNGSGLVPVTQVLKDIGITDITIVPEQEYPNGYFTTCSYPNPEI
                     FAALELGLNLAKETGADLMLATDPDADRVGIAMKCPDGSYELVTGNEVGVLLLDYICA
                     GRIEKGTMPKNPVAVKSIVSTPLADAVAEHYGVELRSVLTGFKWIGDQIAQLEAADEV
                     DRFIFGFEESYGYLAGPYVRDKDAVIGSMLICEMAAYYRSIGSSLKQRMEEIYAQYGR
                     YLNKVDSFEFPGLSGMDKMSALMQGLRDKPLTEIAGHTIVKVTDYQKPEETGLPAANV
                     LIYKLDNGETVVVRPSGTEPKIKIYYTTLGKNLEEAEAEKEKLAEALKPIMA"
     gene            complement(9535..10422)
                     /locus_tag="ACZPD3_00035"
     CDS             complement(9535..10422)
                     /locus_tag="ACZPD3_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1414440.1"
                     /translation="MTNHLFELAGNRKTETVIPKSKKKWYQGKPVVSAVILILIVLGC
                     LCAELIMTKDPTYMDLLNYNKAPDREFLFGTDTMGRDIFSMIWYGGRISILIGGLATV
                     ISTFIAVVVGAFSGVAPGWLDELIMRFTEIFLSIPTLLLIILLQAIMGDANILSLSFV
                     IGVTSWTSIAKVVRTEVRQIRNSEYIIAARCMGAGFFRILWKHLVPNFFSSIMFMVVM
                     NVRTAMISEATLSFMGIGLPIEVITWGSMLYLSDKALMTGSWWIILIPGLFLITTVLC
                     LTNIGNACREQTNRKESNF"
     gene            complement(10419..11423)
                     /locus_tag="ACZPD3_00040"
     CDS             complement(10419..11423)
                     /locus_tag="ACZPD3_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1414441.1"
                     /translation="MRQKKLVYYGKKCLIFLISVFILSVAVFYVARLAPGDPLISYYG
                     DRTEKMSPEEREWAMGKLGLNEPISVQYVKWVKNAFHGEFGISFKYKQDVVEVIGGRI
                     GNTLVLGGIGFIIMFAGALLLGILCAWYENRLADRILCKLGTISSCIPEFWMALVLIL
                     VFSVNLKILPSSGAYSTGNAGDIGDRVLHLIMPLTIVVMEHLWYYAYMIRNKILEEVR
                     ADYVLLAKAKGLDKKKLMFRHCVRNVMPAYLSIMAIAVPHVLGGTYVVESVFSYPGIG
                     ALSYESARYHDYNLLMLLCMMSGILVIFCNIVSQTINEQIDPRMKDEVITEKSEVVEG
                     "
     gene            complement(11423..12334)
                     /locus_tag="ACZPD3_00045"
     CDS             complement(11423..12334)
                     /locus_tag="ACZPD3_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414442.1"
                     /translation="MSKEVLLDVRHLAQQFHLTKKIKVKAVDDVSFQIHKGEIFGLVG
                     ESGSGKSTVARCLMNIYQPAQGEIWYKDVNICDKKEFKKHKKMLQTRRQMIFQDSASS
                     LNQKMKVSDIIAEPMRIQHITPPRGSFVKEAAFQMEYVGLENSFLDRYPSELSGGQRQ
                     RVAIARSLCMEPEFLVADEPIASLDVSIQAQIVNLFRHLQEEHGFTFLFIAHDLAMVE
                     YLCDRVGVMYHGKLVELAPSEELYSNPVHPYTKTLLSAIPVPDPIRERKRVLQYYDGE
                     GMENSVWTEVSPGHFAMLPAEGKGCSI"
     gene            complement(12327..13340)
                     /locus_tag="ACZPD3_00050"
     CDS             complement(12327..13340)
                     /locus_tag="ACZPD3_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414443.1"
                     /translation="MEHLLEVKNLSVSINGPAGEVQAVRDVSFSLKKGEVLAIVGESG
                     CGKSVLCKSIMKLLPPSAKIKEGSICVNGQDITCYREKEMQKLRGRVFSMIFQDPMTS
                     LNPTIKIGKQIGEAVVIHNKNYTKEQVNKKVLELMELVGISHPKERYHQYPWQFSGGM
                     RQRCVMAIALAADPDILFADEPTTALDVTIQAQILDLLREIQMKLGTATILVSHDLGV
                     VARVADRVAVMYAGKIVEIGTAEEVYYDPRHPYTWGLMRSLPAFANGKESLYTIPGMP
                     PTLIDPPKGDAFACRNEYALNIDYEEMPPMFKITDTHYAATWLLDPRAPQIKSPMGGT
                     AHE"
     gene            complement(13486..15027)
                     /locus_tag="ACZPD3_00055"
     CDS             complement(13486..15027)
                     /locus_tag="ACZPD3_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1414444.1"
                     /translation="MKKGLLTMAGVLLTVSLVSAGTTVPVLAEEETTLVYGSGDYTRI
                     NPAMDEHGEINILIFNGLTAHDGDNQVVPGLAESWDFDDETNTYTFHMAEDAKWQDGE
                     PVTAEDVKFTIEAIMDPENGSENAPNYEDVEEINVIDDHTVAFKLEDKNVAFLDYMTM
                     AVLPKHLLEGEDMQTSDFFRNPVGTGPYKIESWDEGQVITLVKNEDYFKGEPSIDKVV
                     FKIVPDDNAKALQLKSGELDMALLTPKDAATFADDDAYTCYDMKTSDYRGIMFNFGNE
                     YWQKNRDLIPAVCYGLDRQAIIDAVILGQGMPAYGPLQRNIYNYEDVEHYDYNPEKAK
                     EILEAAGCEMGDDGFYYRDGEKVGFVISVSAGDQVRIDMAQIAAQELKEIGMDVSVEI
                     PAQTDWAGQMAFLIGWGSPFDADDHTYKVFGTDKGANYSGYSNADVDKYLKEARQSAD
                     PEVRAEAYANFQKALAEDPAYAMICYIDANYVADSNIKGIDPDTIMGHHGVGIFWNVA
                     DWTIE"
     gene            complement(15302..17338)
                     /gene="lysS"
                     /locus_tag="ACZPD3_00060"
     CDS             complement(15302..17338)
                     /gene="lysS"
                     /locus_tag="ACZPD3_00060"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR1414445.1"
                     /translation="MAQQKQEQDLNQLLKVRRDKLADLQANGKDPFQITKFNQTHHSM
                     EVKSLYEAHEAELLKDRAEVDVTGLDEEQAKEAVKKDYEERREIMDASPIHVAIAGRM
                     MFKRVMGKASFCNIQDLQGNIQVYVARDAIGADSYADFKKSDIGDIFGLEGFAFRTRT
                     GEISIHAESMTLLSKSLQILPEKFHGLTDTDMRYRQRYVDLIMNQDSKNVFIKRSQIL
                     KEIRNFLADRDFMEVETPMLVANAGGAAARPFETHYNALNEDVKLRISLELYLKRLIV
                     GGLERVYEIGRVFRNEGVDTRHNPEFTLMELYQAYTDYEGMMELTESMFRYLAEKVCG
                     SAKISYNGIEIDLSKPFERLTMNDAIKKYAGIDFDEVADDEAAKKLADEHHIEYEERH
                     KKGDIINLFFEEYCEKELIQPTFIMDHPIEISPLTKKKPSDPNKVERFELFINTWEMC
                     NAYSELNDPIDQRERFKAQDALADAGDEEANHTDEDFLNALEIGMPPTGGIGYGIDRL
                     VMLLTDSQAIRDVLLFPTMKSQGAAKNEANNVAQAKTVSEKPVEKIDFSKVEIEPLFK
                     DEVDFETFSKSDFRAVKVKECVAVPKSKKLLQFTLDDGTGVERTILSGIHAFYEPEEL
                     VGKTLIAITNLPPRAMMGIESCGMLLSAIHEEEGEEKLHLLMVDDHIPAGAKLY"
     gene            complement(17738..18220)
                     /gene="greA"
                     /locus_tag="ACZPD3_00065"
     CDS             complement(17738..18220)
                     /gene="greA"
                     /locus_tag="ACZPD3_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR1414446.1"
                     /translation="MEEKKNLLTYAGLKKLEEELHDLKVVKRKEVAEKIKEAREQGDL
                     SENAEYDAAKDEQRDIEARIEEIEKILKNAEVVVEDEVDLDKISVGCKVKVHDFEFEE
                     DIELKIVGSTEANSLEGKISNESPVGKALIGAHTGDVVEVEMPAGIMKYKVLEIQRNV
                     "
     gene            complement(18449..19405)
                     /gene="dusB"
                     /locus_tag="ACZPD3_00070"
     CDS             complement(18449..19405)
                     /gene="dusB"
                     /locus_tag="ACZPD3_00070"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR1414447.1"
                     /translation="MQWKIGNVQIDNPFVLAPMAGVTDLPFRTLCKEQGAGLICMEMI
                     SAKAISFHNKNTIALMEIDPCEHPVSMQLFGSEPDLMAEVAKSIEDRDFDILDINMGC
                     PVPKVVNNGEGSALLKNPNLIEEIVRKVSSAISKPLTVKVRIGFENEPVDIVEIAKRI
                     EDAGAAALAVHGRTRQQYYSGTADWDTIRRVKEAITIPVIGNGDVDSPLKAEALVKQT
                     GCDAVMVGRAVRGNPWLFRELNHYFRTGELLERPSVEEIREMILRHARKQIELKGEFV
                     GIREMRKHVAWYTAGMRHSAGLRRESNTIESYEALEALLSRL"
     gene            complement(19458..19823)
                     /locus_tag="ACZPD3_00075"
     CDS             complement(19458..19823)
                     /locus_tag="ACZPD3_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HIR1414448.1"
                     /translation="MYQDNITLCGASAYEKKFYFNQDFNALPDHVKKELQIMCVLYTE
                     DVGGILTLEFDENGRLQFKTEALEADARYDEIGSGLKIKQLQQDKKELLESLEMYYKV
                     FFLGDIPEEELKKTSGSED"
     gene            complement(19862..20665)
                     /locus_tag="ACZPD3_00080"
     CDS             complement(19862..20665)
                     /locus_tag="ACZPD3_00080"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1414449.1"
                     /translation="MAHLYNEETISQAIDTKWAGKTVHFAKETDSTNSWIKRLAKDGA
                     EHGTLAVAEFQSAGRGRFDRRWEAPEGSSIMMTLLLRPEFSPQYASMLTLVMGMAVAQ
                     AAEELGFNVSIKWPNDIVISKKKICGILTEMGTNGVKINYVLIGVGINVNLKEFPEEM
                     QDKATSLILEGGHEYDRNQVIALVMKYFEINYEKFIQTCDFTHLLDDYHRILANLNQP
                     VRVIDGDRSFEGICRGIDEKGELLVERQNKEVVKVSAGEVSVRGLYSYV"
     gene            complement(20665..21666)
                     /gene="argF"
                     /locus_tag="ACZPD3_00085"
     CDS             complement(20665..21666)
                     /gene="argF"
                     /locus_tag="ACZPD3_00085"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR1414450.1"
                     /translation="MNLKGRNFLTLKDFTPEEITYLIDLSAELKAKKKAGELHEYYRG
                     KNIALIFEKTSTRTRCSFEVAAHDLGMGSTYLDPTGSQIGKKESIADTARVLGRMYDG
                     IEYRGFGQEVVEELAKHAGVPVWNGLTNEYHPTQMIADMLTIREHFGDLKGRKLVYMG
                     DARYNMGNSLMIACTKLGMHFVACTTKKYFPNAELVAQCEEYAKASGGSITLTEDVQE
                     GTKDADVIYTDVWVSMGEPDEVWTERIHDLTPYKVTKDVMKNAGEKAIFLHCLPAFHD
                     LKTKIGKEMGERFNLTDMEVTDEVFESEQSKVFDEAENRMHTIKAVMVATLGEPENK"
     gene            complement(21703..22644)
                     /locus_tag="ACZPD3_00090"
     CDS             complement(21703..22644)
                     /locus_tag="ACZPD3_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR1414451.1"
                     /translation="MGVVFLIVIIVIAVWVLASCVRIVPQAYAVILERLGAYQATWST
                     GIHFKVPFIERVARKVNLKEQVVDFPPQPVITKDNVTMQIDTVVFFQITDPKLYTYGV
                     ENPIMAIENLSATTLRNIIGDMELDETLTSRETINTKMRASLDEATDPWGIKVNRVEL
                     KNIIPPAAIQDAMEKQMKAERERREAILIAEGQKKSTILVAEGKKQSAILDAEAEKQA
                     AILRAEAQKERMIKEAEGQAEAVLKVQNANAEGIRMIREAGADEAVLTLKSLEAFARA
                     ADGKATKIIIPSDIQGIAGLASSLKEIVTDPKAETDK"
     gene            complement(22647..23081)
                     /locus_tag="ACZPD3_00095"
     CDS             complement(22647..23081)
                     /locus_tag="ACZPD3_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR1414452.1"
                     /translation="MQPLIWLGILALLLVVEAITAGLTTIWFAGGALVAAIACYAGAN
                     LTVQILLFLCVSLVLLIFTRPLAMKYFNKETIQTNANSLIGKKAVVIQEIDNLAQTGQ
                     VRINDIEWTARSADDEKIGEGTVVTIEEIRGVKLIVKQNKED"
     gene            complement(23102..23875)
                     /locus_tag="ACZPD3_00100"
     CDS             complement(23102..23875)
                     /locus_tag="ACZPD3_00100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1414453.1"
                     /translation="MITSVNNGQVKNIIQLNQKTKARREQGLFVAEGRKMFGEAPRDW
                     ISKVYVSEALSGDAELMAQVEKLPYEIVTDSVFRQMSDTQTPQGIMTVLKKPSYIMED
                     ILGGKNPLVMILEDLQDPGNAGTILRTGEGAGVSGVLLTKTCVDITNPKVIRSTMGSV
                     YRMPFLYVESVVSLAQELKDRNIRTFAAHLHGKNSYDQESYTGGTAFLIGNEGKGLTD
                     EAADSADCLIRIPMCGKVESLNAAMASGILMYEAARQRR"
     gene            complement(23885..24538)
                     /locus_tag="ACZPD3_00105"
     CDS             complement(23885..24538)
                     /locus_tag="ACZPD3_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR1414454.1"
                     /translation="MKNKSYAVIGLGQFGMTVALTLAEANCDVLAIDDKDDNVQDIAE
                     KVSYAVKADVRDPGILESLGVQNVDVAVIAVAENMEASITATMQVKELGVPFVMAKAM
                     NPLHGRILEKIGADKVIYPEHSMGIRVARNLLSSGFVDMFELSSDFSMAEFKIPREWI
                     GKTLRELKVREKYNINLIGLKHGDKMNMNVAPDEVFPADCTVVAAGANSDLNKVSEN"
     gene            complement(24613..26007)
                     /locus_tag="ACZPD3_00110"
     CDS             complement(24613..26007)
                     /locus_tag="ACZPD3_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1414455.1"
                     /translation="MNKKMQLYNRLHKLNTAQIITLGFAGVIILGGLLLWLPFCTAPG
                     YHTSFTDAMFTATTSICVTGLVTVVTATHWTLAGKLIILVLIQIGGVGLISLGSIIFI
                     SLRKKISLRNRRVIQESYNMDRMGGMVRLVKKVLICVFGAEGIGAVCYAVRFIPQFGL
                     AKGLGYSVFTAVSAFCNAGIDLLGEDSLAQYVADPIVNFTSVGLIIMSGLGFVVWWDI
                     WDKIKRVIRGKLPVGRVFKNLRLHSKIVLMMTLILVVGGTVLIFLFDHGNPESIGTYS
                     PGTKWMASLFQSVTTRTAGFFTVSQERFSNATYMLCLILMLIGGSPMGTAGGIKTTTV
                     AVLLLSLKSNLQGKRDVEVHHRRIRDSYIRSAIVVTGMVLTVLILMSMLLCAAMPEAP
                     VEDVVYEITSAVATVGLSRGLTPCLNTAGKWIVILTMYLGRIGPLTLGTAVTVRVQKM
                     PADSHLAEEDIMIG"
     gene            complement(26011..27009)
                     /gene="pfkA"
                     /locus_tag="ACZPD3_00115"
     CDS             complement(26011..27009)
                     /gene="pfkA"
                     /locus_tag="ACZPD3_00115"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1414456.1"
                     /translation="MAENKIKTIGVLTSGGDAPGMNAAIRAVVRRGLSNGLNVKGILK
                     GYNGLLNEEIIDMSAKDVSDTIERGGTILYTARCAEFRTEEGQKRGAEICRKHGIDGL
                     VVIGGDGSFAGAQKLANLGINTIGLPGTIDLDIACTEYTIGFDTAVNTAMEAIDKVRD
                     TSTSHERCSIIEVMGRGAGYIALWCGIANGAEDVLVPEKYDYDEQKLINNIIESRKKG
                     KKHHIIINAEGIGHSEAMAKRIEAATGIETRATILGHMQRGGSPTCKDRVYASMMGAL
                     AVDLLIAGKTCRVVGYRHGEFVDFDINEALAMQKGISDYQWEVCQSLSHNYDKNNK"
     gene            complement(27082..30549)
                     /locus_tag="ACZPD3_00120"
     CDS             complement(27082..30549)
                     /locus_tag="ACZPD3_00120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR1414457.1"
                     /translation="MEFTHLHVHTEYSLLDGSNKIKEYVARVKELGMDSAAITDHGVM
                     YGVIDFYRAARAEGINPILGCEVYVAPGSRFDREAGSGEDRYYHLVLLAENNQGYANL
                     MKIVSKGFTEGFYYKPRVDLAVLKEYHEGIIALSACLAGEVARYLQRGMYEDAKAAAL
                     RYQDIFGKGNFFLELQDHGIPAQRLVNQELLRMHEETGIDLVATNDVHYTRAEDADPH
                     DILLCLQTNKKLADEDRMRYEGGQYYVKSPEEMAELFPYALEALENTHKIAQRCHVEI
                     EFGVTKLPRFDVPDGLTSWEYLNKLCFEGLEERYQPVTEELKARLNYELSTIKNMGYV
                     DYFLIVWDFIKYARDHDIMVGPGRGSAAGSLVAYTLGITQLDPIRYDLLFERFLNPER
                     VSMPDIDVDFCFERRQEVIEYVRRKYGDDCVVQIVTFGTLAARGVIRDVGRVLDMPYA
                     QVDSIAKMIPQELNITIDKALTMNPELKKAYEEQDEIHYLIDMARRLEGLPRHTSMHA
                     AGVVISQKDVSEYVPLSRASDGSIVTQFTMTTLEELGLLKMDFLGLRTLTVIQNAVKL
                     IQKDAGVTLDMQKINYDDKKVLDSLGTGRSDGVFQLESAGMKNFMKELKPQSLEDVIA
                     GISLYRPGPMDFIPQYIRGKNRPDTIRYDCPQLEPILKPTYGCIVYQEQVMQIVRNLA
                     GYTLGRSDLVRRAMSKKKASVMEKERQNFVYGNEAEGVPGCIANGIDEATANKIYDEM
                     IDFAKYAFNKSHAAAYAVVSYQTAYLKYYYPVEFMAALMTSVIDFPNKVAEYILVCRQ
                     MGIKILPPDVNCGMYGFSVDNGAIRYGLSAIKSVGRPVIESLVREREENGQYRSLKDF
                     MERNSPQMNKRAVENFIKAGALDCLDGNRRQKMLVYQKISDSISQDKKNSLAGQMSLF
                     DLVSEEDKKEFEIRMPDVEEFGKEELLGYEKEVLGIYLSGHPLENYREMMEKTISAKT
                     SDFQQDEETNLPKVMDGQKVIIGGMITDKTIKYTKNNKVMAFLTVEDLVGTVEVVVFP
                     RDYEKSQQFLNEEGRVFIQGRVSAEDDRASKLILEKIRPFDNMPREIWIQFDNKESYT
                     QQSQELLADLRRSPGDSAVVIYLKDVKAIKKLPVGYHAQIQDSWLNYMYEKYGKTNVK
                     VVERGLKNL"
     gene            complement(30683..31522)
                     /locus_tag="ACZPD3_00125"
     CDS             complement(30683..31522)
                     /locus_tag="ACZPD3_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvfB family protein"
                     /protein_id="HIR1414458.1"
                     /translation="MIELGKKQKLTVVKSVDFGVYLGEDMQADAKNRVLLPSRQVPEG
                     TKEGDSIEAFIYKDSQDRLIATTKEPKLQVGQTAVLKVSQVTRIGAFLDWGLEKDLLL
                     PYHEQTLKVREGEDVLVALYIDKSSRLCATMKVYHYLSTRTPYVVGDMVKGRVYEISD
                     RFGVFVAVDDKYSALIPAREAKGKYRPGKILELRVSEVKEDGKMNVTDRQKAYLQINE
                     DAENVLEVINEFAGVLPFDDKASPEVIQREFGLSKGAFKRAIGHLMKEGKVEIKDKRI
                     YAK"
     gene            complement(31758..32576)
                     /locus_tag="ACZPD3_00130"
     CDS             complement(31758..32576)
                     /locus_tag="ACZPD3_00130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1414459.1"
                     /translation="MIYKKIEIAVDGYKETADLYTYFLDNSIEMHINRKRPVVVICPG
                     GGYAMTSDREAEPIAMQYLVRGYHAVILRYSVEPARYPLALLQLAKSVAFLRKNAAEF
                     HIDTNKIVLQGFSAGGHLAASLGVFWKKDFIAQTLGVTSDMVKPNGMILSYPVITSGE
                     FAHTGSFECLLGEDYNDLDKRKEQSLEFQVSKDTPTTFLWHTVTDDCVPVENSLLFFN
                     ALRKFEIPVEMHLYPVGGHGLSLANEETSHEDGGCVQKECQSWIELACKWMQNI"
     gene            complement(32566..33792)
                     /locus_tag="ACZPD3_00135"
     CDS             complement(32566..33792)
                     /locus_tag="ACZPD3_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414460.1"
                     /translation="MKEQQEKIRVLDPHIILILFISAFVFGIGMLMIFLERKEIRTEG
                     YEFIIFSVEILMLLLILYYMRTVVLSREGCTVSWLFWKRNYKWEELAVIREDVWYTYG
                     KGGSIRYQGIVFSKYAVNKKKKKYTTRMIVDAFPLSDCFYITFDEHGNVFSYKLEKGK
                     KQVIRKMYVNVREKMKEWGVEAEKGEGIKEEEKQRQYDEMIELRRQQRKKIQSKGADC
                     TNLKEYQQEVIKIRDFLQITMVILITVLLIWTGLSAEEGERIPILILEFLIDAPLLFF
                     SSKVVTLSKEGCKISYLFFKKMYKWEELEIIREDYFPSFRDRRSRGIVFSKRKKNKAG
                     YIYTTREIVGSGHILECFYIVLDADGNIHLYKRLAAKDGKTKLRKVAVNIPQKLAEWG
                     VEMEKMMKYTGGTYDL"
     gene            complement(34213..34905)
                     /locus_tag="ACZPD3_00140"
     CDS             complement(34213..34905)
                     /locus_tag="ACZPD3_00140"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     D-xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR1414461.1"
                     /translation="MLEKLKEEVYKANMDLPKYGLVTFTWGNVSGIDRESGLFVIKPS
                     GVDYDLLTPDDMVVVDLNGNKVEGKYNPSSDTATHVELYKAFPNIGGVVHTHSSWATS
                     WAQAGRSIPCYGTTHADYIYGEVPCARCLEGKEFEEYEKNTGLLIVDLFKDKDYEAVP
                     AVLCKNHGPFAWGKDAHEAVHNAVVLEEVAKMASRCELINPQVKPAPQDLQDKHYFRK
                     HGANAYYGQGNK"
     gene            complement(35057..35887)
                     /gene="rhaD"
                     /locus_tag="ACZPD3_00145"
     CDS             complement(35057..35887)
                     /gene="rhaD"
                     /locus_tag="ACZPD3_00145"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HIR1414462.1"
                     /translation="MKILESKFVQGFIKMANDGYLQGWHERNGGNLSYRLKPEEVEMI
                     RPRLNTPGEWIPIGVEVPGLAGEFFLVTGSGKYFRNIIVDPEVCLAIIELDETGTNYR
                     IRWGLVEGGRPTSELPTHLMNHEVKKKLTNGKHRVIYHAHTTNTIALTFVLPLDDKIF
                     TRELWESATECPVVFPDGVGVVGWMVPGGREIAIKTAELMKKYDVVIWAHHGMFCSGE
                     DFDLTFGLLHTVEKSAEILVKILSMTPNKLQTITPDNFRSLAKDFKVSLPEEFLYEKQ
                     "
     gene            complement(36095..36805)
                     /locus_tag="ACZPD3_00150"
     CDS             complement(36095..36805)
                     /locus_tag="ACZPD3_00150"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016297 - fatty acyl-[ACP] hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HIR1414463.1"
                     /translation="MNYSFNSRVRYSETGENGKLTLPGVLNYFQDCCTFHAESVGLGG
                     DVLKARDRAWVLSSWQVIVDEYPAMGTEIRITTAPYDFKGFMGMRNFTIETMDGKKLA
                     WANSNWTHLAISTGIPVRLTPADTDNYILGEKLEMDYAPRKIKLPDDMTSQESFTVQK
                     HHLDTNHHVNNCQYICMAEDFLPEDFKVYQMRAEYKMQAKLGDIICPKAKAETGKVIV
                     SLDDTDGKAYAIIEFQQK"
     gene            complement(36941..39598)
                     /locus_tag="ACZPD3_00155"
     CDS             complement(36941..39598)
                     /locus_tag="ACZPD3_00155"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414464.1"
                     /translation="MKGKGYRSSSVKAIWIVIAHLAAVAAAVCAAMFVMIYQTGIRLD
                     DRGKSYTESEAFEKQVSNRGSDILVSLAAQDDINYLKNAGSSAVIDLAEFEEKGNTRD
                     SIRDLSLKNTSGLAYSVSDLLEWGKDWEANYYEGVYDEDSQVIRCESSDGTSHYFYRT
                     DFKKMVADGTLKINYNTDFLEEDDFESKTESEKLDTVADELYYRYTSQSENIGNVTDT
                     RTNTEYPGCFFVELSQLDEKFAPQGAENILDAVNKSTEWNGRLEDAYKELFTLLDCIR
                     AIQSDEQFNDYETSLASVFHSVGDYTEGSTNLTYLFADKETQTIYTNKKAYSSYAQLE
                     QNLEKIFKEKAYAVVYPELSECVTNIPGADLQVWNHTIDQSFDTKDFVFAVSVDTKFS
                     VADSMADEAENYETYSKLMFPMLAGAIFGSVLWLIGMVWLTVTAGRRPEDEEIHLNGF
                     DRWYTEIAAGTVIGIWLAGTIISGTLIANSSLGYSHVVVTVIVICLICGTYTMAWFLI
                     GYLSLVRRIKAGTLWKNSLIRKVLKWIGKCSGKLADFARAFSRNTAEKIKVLLVGGAF
                     LFLQFLIIGCVFSGAGVFLLALMAVDVAVMIFAIRKADGLDLIMDGLKKISDGELQYK
                     IKTDTLTGKQKVMAEYINNIGSGLDAAVENSLKKERMQTELITNVSHDLKTPLTSIIN
                     YVDLMKRENPTDPKIQEYLRILDEKSQRLKVLTEDVVEASKASTGNIKLEMNDIDFVE
                     MVQQVIGEFEEKFQEKNLTMMVHFTDEPSIIYADGQRMWRVLENVFGNVVKYAMEGTR
                     VYAEISNRNKKVTFSLKNISAQPLNISADELTERFIRGDVARNTEGSGLGLSIAKSLT
                     ELQGGEFKLYLDGDLFKVMITFVAKNYSK"
     gene            complement(39797..40492)
                     /locus_tag="ACZPD3_00160"
     CDS             complement(39797..40492)
                     /locus_tag="ACZPD3_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1414465.1"
                     /translation="MANILVCDDDKDIVEAISIYLEQEGYTVLKAYDGVEAINALRSQ
                     SVDLLIIDIMMPKLDGIRATLKIREENPLPIIILSAKSEDADKILGLNVGADDYVTKP
                     FNPLELVARVKSQLRRYTRLGAAIPVENAHIYETGGLSINDDLKEVRVDGDVVKLTPI
                     EYNILFLLMKNQGRVFSINQIYENIWNEEAVAADNTVAVHIRHIREKIEINPREPRYL
                     KVVWGLGYKVEKI"
BASE COUNT        10190 a        10069 c         7395 g        12888 t
ORIGIN      
        1 tgtcaattac agtttcttaa cacccatagt tactgcttct ttgttgatgt tccactcttt
       61 cacataacca tcggtctcgc caagtaccat ttcgtctgca agtacttctg acttgatctc
      121 atcttcgtgt gttttcatga catcctggat cttgtcgttt ccgtgagcgt atactacgat
      181 cttgtccata acttcgaagc cagcttcttt tctcatggtc tggatcttgc tgatgatctc
      241 acgtacaaat ccttcttcga tcagttcagg tgtcaggttt gtatccagga ctacggatgt
      301 ttcaccgtca gattcagata cataaccctc tgtctgtgct gtttcaataa gaaggtcttc
      361 ttctgtaagc tctacatcgt ttccgttgat atccagtttc aggacaccgt tggttctcaa
      421 ctcgttcatt gctgctgttc cattgatgtc tgtgagagcc tgtctgattc ctccaaggag
      481 tttaccgtat ttcggtccta ctgtacgaag ctgcggtttg aagctgtagg agataaagga
      541 ttctacatca tctgcgaatt ttacttcttt gacattcagt tcgtctgcga tgatgtcagt
      601 gtagaattca ctcattttct tctcagcttt tacatacata gttccgatcg gctgacggtt
      661 cttgatgttt gctgtatttc tgcaggcacg tcccagaact acgatctcca gaagctcttt
      721 catgtctgct tccagatctt tgtcgatcca tgcttcgttt acagttggga agtcgcagag
      781 atggatactc tccggagcat ctttatccac gcttcttacc aggttctgat agatgtcttc
      841 tgtcatgaac gggatcatcg gagcagctgt cttggcgatg gttaccagtg catggtacag
      901 agtcatgtaa gcattgatct tatcctgctc cattcccttt gcccagaaac gttcgcggct
      961 tcttcttaca taccagttac tcatttcatc tacgaattcc tgaagtgctc ttgctgcttc
     1021 cgggatcttg tagttagcca gatcattgtc tacagcctga acagttgtgt ttaatctgct
     1081 gaggagccat ttgtccataa caggaagctg gtcatagttc agttcatatt tggtcgggtc
     1141 gaagttgtcg atgtctgcat ataatacgaa gaatgcgtaa gtattccaca gtgtactcat
     1201 gaatttacgc tgtccttcca caactgcctt gccatggaaa cggttcggaa gccatggagc
     1261 actgttgatg tagaagtacc atctgatggc atctgcgccg tatttgttca gtgcatcgaa
     1321 tggatcaact gcatttcctt tggacttact catcttctgt ccgttctcat cctggacatg
     1381 tcccagaacg attacgttct tgtatggtgc tttgttgaag agcagtgtgg attctgcaag
     1441 gagtgagtag aaccatccac gggtctggtc tactgcctcg gagataaagt ttgcagggaa
     1501 ctgctgctca aataattctt tgttttcaaa tggataatga tgctgtgcga aaggcattgc
     1561 tcctgagtcg aaccagcagt caataacctc cggtacacga tgcatttctc caccgcactc
     1621 cggacacttc agtgtaatct catcaatgta tgggcggtga agctctactg tcttggctct
     1681 ctcatcaccg ctcatctcga ataactcctg acggcttccg atggaatgca tatgtccgca
     1741 ttcacactgc cagatgttca gtggagttcc ccagtaacgg ttacggctga taccccagtc
     1801 ctgaacgttt tcaagccagt cgccgaaacg tcctttaccg atgctctcag ggatccagtt
     1861 gattgtattg ttatttctga tcaggtcatc cttaactgct gtcatcttga tgaaccagga
     1921 ttctcttgca tagtagatca gtggtgtgtc acatctccag cagtgtggat aatcatgctc
     1981 gaatttcgga gcatcgaaga gcttgccttc tttgtccaga tctgtgagga ccataggatc
     2041 tgctttcttt acgaaaacgc ctgcgtatgg tgtctctttt gtcaggttac cctgtccgtc
     2101 tacgaactgt acgaatggca gttcatagtt acgtccgatt ctgctgtcgt cttcaccgaa
     2161 tgcaggtgcg atgtgaacga tacctgtacc atcactcata gtaacgtagt tatcacaggt
     2221 tacgaaatga gctttctttt tctgttttgc tgctgcctct cctgcacatg cgaaaagcgg
     2281 ctcgtatgct ttgtattcca ggtctgttcc tttgtaggtt tccagaactt catatgcttt
     2341 ctcgccttct tcttttacaa gtttgccaag gactttgtca aggagtgctt ctgccatata
     2401 ataggtatat ccgtctgctg cctttacttt gcagtaagtc tcatccgggt ttacgcagag
     2461 tgcaacgttg gacggaagtg tccatggtgt tgttgtccat gcaaggaagt atgcatcttc
     2521 accaacaacc ttgaatctta caactgcgga gcgctctttt acagttttgt atccctggga
     2581 tacttcctgt gcggaaagag gggtaccgca tcttggacag taaggaacga tcttgaagcc
     2641 tttatacaga agcttcttgt tccagatttc ttttaatgcc caccattcgg attcaatata
     2701 atcgtcatag taagttacgt atggatgttc catatcagcc cagaaaccaa cagtagagga
     2761 gaaatcctcc cacattcctt tatatttcca gacgctttcc ttacactgtt tgatgaatgg
     2821 ctccataccg tattcttcga tctgctcttt gccatccaga ccaagtttct tctctacttc
     2881 cagctctacc ggaagtccgt gagtatccca tcctgcttta cgcggaacca tgtagccctt
     2941 cattgtacgg tatctcggga tcatatcctt gataacgcgg gtaagtacat ggccgatgtg
     3001 cggtttaccg tttgcagtag gcggtccgtc gtagaatgta taggtttctc cctcttttct
     3061 gttctccata cttttctcaa agatatggtt ttctttccag aattcttcta cttttttctc
     3121 acggtctaca aagtttaagt tcgtatcaac tttctggtac acagtaattt acctcctgtt
     3181 taaattattt gtatcagaca cctgaaaata tgcaggacgt ttatatacgc tgaaaaatan
     3241 nnnnnnnnna ggatgcccgt acaatgtaac aggtggttta gcgaacgaaa ataagcccat
     3301 aaaaaaatcg cccttttgta aaaggacgaa ggtttattga cgttcgctat accacctgtt
     3361 acattgtacg ggcatcctct gccgatacat gaatctttta acggggattt ccgtcagctc
     3421 ttacctgtct gtacaccctt cagagctgca actccggagt gatcttcact gcatttactc
     3481 accaccgggc tcacaccctc cccggttcgc tgtctgcttt cccctgcagt tactctctcc
     3541 atcaacgttt ttgtggacat atcatttatt taactatgcg gattcattat aaggtcacaa
     3601 aattcactgt gtcaagtatt tttttgaata ttagggtaat cctgttactg tttaataaca
     3661 ctggttactg tcaaactccc gaacagtaac acactgttaa catactatca tataaatttt
     3721 acacaggcat tgtaattaat gatataataa ggtagaatat tttgtccgaa cgtcatttaa
     3781 aacgatgtac taggatatgg aaagcaacta ctatgtatat ttaatgaagg gtaatttcaa
     3841 atgtttaaaa caaaatttca taaattaatg aaacgtttcc tcaccggaat gctttgtgca
     3901 gggattctga cctctcagcc tgcagtcact tacgctgccg agacctctgc cactcctgaa
     3961 gccggtcatt cagaaaccta ttcacaggcc tcagatatcg attctgtcaa aggatggccc
     4021 acaggtccta atatcgaggg acagtccgca gtgctgatgg atgcagtcac tgatactatt
     4081 ctttattcca agaatgccaa agataagcta tatcccgcca gtatcactaa gatcatgacc
     4141 gctcttctgg cctgtgaata tctggatatg aacgatacga tcaccatgtc gcaggaagcg
     4201 gcttatggga ttgaggccgg aagctccagc atttacgcag aaacaggaga agttttcaca
     4261 gtagagcagg ccctgatggc tctgatgctg gaatccgcca atgagatggc tcttgctctg
     4321 gcagaaaaaa caagcggttc tgtaaagaag tttgttgaac tgatgaacca gagggcagct
     4381 cagcttggct gtaagaacac ccattttaat aatccaaatg gtcttccgga tgagacgcat
     4441 tataccaccg caggtgatat gatgaagatt gcaaaggcag cctggtataa tcccagattc
     4501 cgtaaatttg tgaccacaca ggtttatgag attcctccga ctaataagca gtctgaaacc
     4561 cgttatcttc tcaatcacca taagatgatg cccggacagt cttatgcata cgatggggtt
     4621 ctgggaggaa agaccggata caccgatgca gcgggaagta cgcttgtcac ctatgccaag
     4681 agaggaaatt ccattctcat tgcagttgtg ctgaattcta caaacggtgc ttttcctgac
     4741 acaacttctc tgctggatta cggcttcgac aattttgaaa aagtagactt gaatatagat
     4801 accgatccgg taccggcagt atttctccca tgcgaaaagc acctgttaaa ggactggaat
     4861 aatctgtgtt ccttctatta tatgaggcat gtgtacgtaa ctgtccccac aggaaccgat
     4921 gtgagccagc ttgtaaagaa gcagaaactt ctgaacaatt ctgtcggtcc aaagcgaatt
     4981 aaaagtaaat attatttaga cggccatatg gtcggatatg gaatgcaata tgaaaaggag
     5041 atcctttcag atttccttct caacgcttct ttttagtttt cgcctgaggc ggaggacttc
     5101 ttttctttca cgaagttcct ctgcttcttc tgtatctgtg aaacgcaatg tcttcacagc
     5161 gaaatatttc tcatcttctg tttttcggat gcggatcttg cctcttatca gtccatgttc
     5221 ttcacaattg gatatgctgt aataaacctt tggattattc ataaaccatc ggagtttgcg
     5281 ctttgcaggc agatgacaga ccggacatct ggtgctagtc acctcgcggt ctttcatgat
     5341 tcgctctctt gtggcaaatt ctctggatac atatttgtca tgatccggat aggaaatatg
     5401 tatctcgtct tttttcttct ttggattctg gtaaacatcc agtgacggat gattgatgat
     5461 gattatgtta tttattttct ccaggacttt cgctgtatac caggcatctg caagcgcccg
     5521 gtgaaatccc ctggatttct ctattttcag ctcatcgatt gcatgctcca atgcacacct
     5581 gtgtgtaccg tcatcataac tgatactgta tagtttctgc acatcataat aggtcacagg
     5641 tccgggaagc agatccagca ggccatagaa cttcatattt ctctgaagtt ccatcacgtc
     5701 ctgatttccc caggtaaaaa agtaccagtc ttctccgcac cagtcaataa attccctgac
     5761 tgcctgctca aagggcactc ctttcataag atctttataa tctacatgaa ttacctcatg
     5821 gatgctgtca tgaatccatt tatagacctg tggtttgatc agtcggtgaa aatgatccgt
     5881 tatttctttt ttgctgttta cttttacagc cccaatctcg ataatctcaa atggaagcct
     5941 gtcgttacgt gttttctttc ccgaaggatt ctgattccat tccaggtcaa agacaatata
     6001 gttcattcat ccagtccttt ttgttcatta ctttttcgca tttattttcc actgctgata
     6061 aacatattat ttcacaatct tcagttcata ctgtctggtt cccagaccaa tcttctctgc
     6121 ctgatccagt gttgcctgcc attcaatgtt cgggttggaa tctttgaaat ggtcgtggtg
     6181 gcaatgccag tcaggttttg ccagatgttc tccaagctgg ctgttctcga tcggagttgc
     6241 tttcagacag gcatctgcgc aggcctgatc cagagcaacc gggtcaaagc ttgcaaacat
     6301 tccgatatca ggaaggattg gtgcatcatt ttcgccatgg cagtcacagt tcgggctgat
     6361 atcctgaaca agtgctacat ggaaatgcgg acggccatga cagactgcct gtgcatattc
     6421 tgccatctta cggtcaagaa gttcattcgc actgctgttt ggattgtata ctgcatcaaa
     6481 accgcaggct ccgatacaac ggccgcagcc tttacattta tcataatcaa tgactgcttt
     6541 tttattcgga tatgtaatcg catcacttcc gcactctttc gcgcagcgac ggcatccgcg
     6601 gcagagttct tcatttactg caggttttcc gctggcatgc tgtttcatct taccagcgcg
     6661 gcttccgcat cccattccga tgttcttgag agcaccgccg aagccggttg cttcatgacc
     6721 tttgaagtgg cttaagctga taaaaatatc tgcatccatg atcgcatgtc cgatcagtgc
     6781 agtctgacaa tattcaccgt taactaccgg tacttctact tcgtctgtac cgcgtaagcc
     6841 atcaccgatg atgatctggc atcctgttga aatgctgtta aatccgttga gatttgcgca
     6901 ctccagatgc tccagtgcat tcttacggct tcccggataa agagtgttgc agtctgtgag
     6961 gaacggaagt cctccctgct ctttacagag atcagctact gcttttgcgt aatttgggcg
     7021 caggaaagcc agattgccaa gttctccgaa atgcatttta atagccacga attttccatc
     7081 catatcaatg tctttgattc cggctgtaac gcagagtttc ttcagtttat caagctggct
     7141 ggtgccaacc ggacatctga aatcagtaaa ataaacggtt gatttctcca tcgttttttc
     7201 ctcctgtttt tattgaccaa aatttgattt tcttttttat tttatcacaa gatgcaggtt
     7261 cttttcgaga tatttttttg cttacatctg ttcgtaaaat tttgtcacct ttttgtattc
     7321 tgcaggcaaa gtaatctgct ctaccacaac atctccatct tctgcattgt ggtaacggta
     7381 aatgtaaacg ccctcctgga tctcatctcc ttcgatcaga gaaactgcca gatatccctg
     7441 tccttcaaat tcaaaggtat ccatgattgc aaaatcccgt tctgttccgt cagataccgg
     7501 gatgctgatt acttcatatt cttccatagt atgttttcct ttcatttata agcagtctta
     7561 ttcttatttt cgctgttttt tattgtagca aagagtgtta attatatcaa tcctatttaa
     7621 ccgaatcaag taagtcgtta ctgttcacag taacagtaag gcccctgcgg gttacaaaaa
     7681 gaaagactgc acctccaatg agcacagcct ttccggttta gtatatttta ttcagttctg
     7741 cttacaattg cttatgccat gatcggtttt aatgcttctg caagcttctc tttctcagct
     7801 tctgcctctt ccaggttctt gccgagagtt gtgtaataga tcttgatctt tggttctgtt
     7861 ccggatgggc ggacaactac tgtctcgccg ttatccagct tgtagatcag tacgtttgct
     7921 gcaggaagtc ctgtttcttc cggtttctgg taatctgtta cttttactat agtatgacct
     7981 gcgatctctg tgagaggttt gtcacgaagt ccctgcatca gagctgacat cttatccatt
     8041 ccgcttaatc ccgggaattc gaagctgtct actttgttca ggtaacgtcc gtactgtgca
     8101 tagatctctt ccattctctg tttcagggaa cttccgatgc ttctgtaata tgcagccatt
     8161 tcacagatca gcatagatcc gataacagca tccttgtcac gtacatatgg tcctgcaagg
     8221 tatccgtagc tctcttcaaa tccgaagata aatctgtcta cttcatctgc tgcttcaagc
     8281 tgtgcgatct gatcgccgat ccacttgaat cctgtaagta cgcttctgag ttctactcca
     8341 tagtgctctg ccactgcatc tgcaagtggt gtggaaacaa tggatttcac tgctaccgga
     8401 ttctttggca ttgtgccttt ttcaatgcgg cctgcacaga tataatcaag aaggagtaca
     8461 cctacctcat tacctgttac cagttcatag gaaccatccg ggcatttcat tgcaatacca
     8521 acgcggtctg catccgggtc agttgcaagc attaagtctg cacctgtttc ttttgcgaga
     8581 tttaatccaa gttccagagc tgcaaagatt tccgggttcg gatagctgca ggttgtgaag
     8641 tatccgttcg gatattcctg ttccggaacg attgtgatat ctgtgattcc gatatctttt
     8701 aatacctgag ttacaggtac aagtcctgat ccgtttaacg ggctgtaaac cagtttcagt
     8761 cctgctgtct tgcagagacc cggacgaacc tgacgggatt cgatagcttc atagagagcc
     8821 tgcttgcagt catctcctac aaaacggatt tttccctgtt ctacgccttc tgcgaaggac
     8881 atatatttcg cacctgtaag tacgtctgtt ttctggatct cttcatatac aatggcagct
     8941 gcatcgtctg tcatctggca tccgtcagga ccatatgctt tgtatccgtt gtacttagcc
     9001 gggttgtggg atgctgttac cataatacct gcattgcatt catagtaacg tgttgcaaag
     9061 gaaagtgcag gtacaggcat cagtgcgtca taaattctga cattgatatc atttgccgcc
     9121 agtacgcctg ctgctgtttt tgcaaataca tcactcttaa gacggctgtc atagctgatg
     9181 gctactgtct gattaccgcc ctgtgttttt acccagttcg caagtccctg tgttgcctgg
     9241 cgaactacat aaatattcat acggttggtt ccggctccaa gtactccgcg aagtccggct
     9301 gttccaaatt tcagcgctac tgcgaaacga tctttgatct cttcatcgtt tccttcgatt
     9361 ttggataatt ccggattaag atccgcatcc tgcagatctg ctgccaacca acgtttgtat
     9421 tcttcctgat acattttgac cactcctgcc ttgtttttta gattttttaa gatattttca
     9481 taatatcata aatcagacaa atcagcaaca agacagcgag atttcaatca aattttaaaa
     9541 gttactttct tttctgtttg tctgctctct gcaggcattt cctatattgg tcaggcagag
     9601 cactgtggtg atcaggaaaa gtcccgggat cagaatgatc caccaggaac ctgtcattaa
     9661 tgccttatct gacagataca gcatacttcc ccatgtgata acctctatcg gaagtccaat
     9721 acccataaaa cttaaagtag cctcagagat cattgcagtt cttacgttca taactaccat
     9781 aaacataatg gatgagaaaa agttcggcac cagatgcttc cacaaaatac ggaaaaatcc
     9841 tgctcccata catcttgcag cgatgatata ctcactgttt cgtatctgcc gtacctctgt
     9901 gcgtactact ttcgcaatac tggtccagct ggtcacaccg atcacaaagg acagactcag
     9961 gatatttgca tctcccataa tagcctgcag caaaatgatc agaagcagag ttggtatact
    10021 taaaaatatc tctgtaaatc tcatgatcag ttcatccagc cacccaggtg ccacaccact
    10081 gaaggcgccg actaccactg caataaaagt agaaataaca gtggctaatc caccaatcag
    10141 gatcgagatc cttcctccat accagatcat ggaaaaaata tcccttccca tagtatctgt
    10201 tccaaaaagg aactccctgt caggagcttt attatagttt aacagatcca tataagtagg
    10261 atcctttgtc atgatcagtt ccgcacacag acagcccagt acgataagaa tcaggattac
    10321 tgctgaaaca acgggttttc cctgatacca ctttttcttt gatttgggaa ttacggtttc
    10381 tgtctttctg tttcctgcca gttcaaataa atgattggtc atccttctac tacctccgat
    10441 ttctcggtta ttacttcatc cttcatacgg ggatctatct gttcattaat agtctgactg
    10501 acgatattac agaaaataac caggattcct gacatcatac aaagcagcat cagcagattg
    10561 taatcatgat atctggcact ctcataggac agagctccga ttccgggata agagaataca
    10621 ctttctacca cataggttcc tccaagtaca tgggggactg caatggccat aatactcaga
    10681 taagcaggca ttacatttcg gacacaatgt ctgaacataa gctttttctt atccaggcct
    10741 tttgcctttg ccaaaagtac ataatctgcg cgaacctctt caaggatctt atttcggatc
    10801 atgtaagcat aataccataa atgctccatg accacgatgg tcagaggcat gatcagatgc
    10861 agcactctgt caccgatatc tcctgcattt cctgtactgt aagctccact gcttggcagg
    10921 atcttaagat ttacggaaaa aacaaggatc aggaccagag ccatccaaaa ctcaggaata
    10981 caactggata tggttcccag cttacacagg atccggtctg ccagccggtt ttcgtaccag
    11041 gcacacagga ttcccaggag cagggctccg gcaaacataa tgataaatcc gattcctcca
    11101 agtacaagtg tattgccaat acggccgccg atcacttcta ccacatcctg tttatattta
    11161 aatgaaattc caaattctcc atgaaaggca ttttttaccc acttcacata ctgcacagaa
    11221 atcggttcgt tcaatcccag ttttcccata gcccattcac gttcttccgg actcatcttc
    11281 tctgtacggt caccataata ggagatcagc ggatctcccg gtgcaagacg ggctacataa
    11341 aatacagcta cagacaatat aaatactgag atcaggaata tcaggcattt tttaccatag
    11401 tagactaact ttttttgtct cattatatac tgcacccctt tccttcagcc ggaagcattg
    11461 caaaatgtcc gggtgacact tctgtccaca cgctgttttc cattccttcg ccatcatagt
    11521 actgcagaac tctttttctc tccctgatcg ggtcaggaac aggaattgca gacagcaatg
    11581 tttttgtata aggatgcacc ggattggaat acaattcttc tgatggcgcc agttctacca
    11641 gttttccatg atacataact cccacacggt cacagagata ctcaaccata gccaggtcat
    11701 gagctataaa cagaaatgta aatccatgct cctcctgcag atgacggaac agattgacga
    11761 tctgtgcctg tatggacaca tccagggaag ctatcggttc atcagccacc agaaattccg
    11821 gttccataca cagagatctg gctatggcca ctcgctgtct ctgtccccct gataattcag
    11881 atggataacg gtccaggaaa ctgttctcaa gtcctacata ttccatctga aaagcagctt
    11941 ccttcacaaa gcttcctctc ggaggtgtga tatgctggat tctcattggt tccgcaataa
    12001 tatctgacac cttcattttc tggttcagac tggatgcgga atcctgaaat atcatctggc
    12061 ggcgggtctg cagcattttt ttatgctttt tgaattcctt tttgtcgcag atatttacat
    12121 ctttatacca gatttctccc tgagccggct gatatatatt catcagacat ctggcaactg
    12181 tggactttcc tgaaccggat tcccctacca gaccaaagat ctcaccttta tggatctgaa
    12241 atgagacatc atctacagcc tttaccttga tcttttttgt cagatgaaac tgctgagcca
    12301 gatgcctgac atccaataaa acttctttac tcatgcgctg ttcctcccat aggtgatttg
    12361 atctgcggtg ctcttggatc caacagccag gttgccgcat aatgagtatc tgttatctta
    12421 aacatcggcg gcatttcttc ataatcaata ttcagggcat attcatttcg acatgcaaaa
    12481 gcatcacctt tcggcggatc tatcaaagta ggaggcatgc cggggatagt gtacaagctt
    12541 tcttttccat ttgcaaatgc aggcagtgat ctcatcagcc cccatgtata tggatgtctg
    12601 ggatcataat acacttcctc tgctgtaccg atctctacga tcttgcctgc atacatgact
    12661 gctactctgt ctgccacacg tgcaaccact cccagatcat gggataccag tatggtagct
    12721 gttccaagct tcatctgtat ttcccgcaga agatccagga tctgtgcctg tatggtcaca
    12781 tccaatgcag tagttggttc atctgcaaat aaaatatccg gatctgctgc cagagcaatg
    12841 gccataacac atctctgacg cattcctccg gagaactgcc agggatactg atgatatcgt
    12901 tcctttggat gggagatacc taccagttcc ataagttcca gaaccttctt atttacctgt
    12961 tccttcgtgt aattcttatt gtggatcacc actgcttccc caatctgttt tccgatttta
    13021 atagttggat tcagggatgt cataggatct tgaaagatca tggaaaaaac tcttccacgc
    13081 agtttctgca tctccttttc cctgtaacag gtgatatcct gtccgttcac acagatgctt
    13141 ccctctttaa tctttgcact tggcggcaaa agcttcatga tacttttgca aagcacactc
    13201 ttcccgcatc cggattctcc aactatagcc agcacctctc ccttttttaa tgaaaaagac
    13261 acatctctta ctgcctggac ttctccggca ggaccgttaa tgctgactga aagattttta
    13321 acctctaata aatgttccat ttttaatcct ttttccgctc gtgtaaaaag ggggcacctt
    13381 tagtgccccc gtcacaacct ttatcttttt tatgatgtta gagtcaaagg tcttatggcg
    13441 caagcatgaa tggctttttg accttttgac cctgatatca ttttattatt caatagtcca
    13501 gtctgctaca ttccagaaga ttccaactcc atgatgtccc ataattgtat caggatcgat
    13561 tcctttaata ttgctgtcag caacatagtt ggcatcaata tagcagatca ttgcataagc
    13621 cggatcctct gccagagctt tctggaaatt agcataggct tctgcacgaa cttccggatc
    13681 agctgactgt cttgcttctt tcagatactt gtcaacgtct gcattagagt agccggagta
    13741 gtttgctccc ttatctgttc cgaatacttt gtatgtatgg tcgtcagcgt caaatgggct
    13801 tccccatccg atcaggaatg ccatctgtcc tgcccagtct gtctgtgcag ggatctctac
    13861 tgatacatcc atgccaatct ctttcagttc ctgtgctgcg atctgagcca tatcaatacg
    13921 tacctgatct cctgcactta cgctgattac aaatcccact ttttcgccgt cacgataata
    13981 gaatccgtca tcacccatct cgcatcctgc agcttccaga atttcttttg ctttttcagg
    14041 attgtaatca taatgctcta catcttcata attatagata tttctctgaa gaggaccata
    14101 agccggcatt ccctgtccca ggataactgc gtcgatgatt gcctggcggt caagtccata
    14161 gcatactgcc gggatcagat cacggttttt ctgccagtat tcatttccaa agttaaacat
    14221 gattccacga tagtcagaag tcttcatatc atagcatgta tatgcatcat catctgcaaa
    14281 agtcgccgca tcctttggtg ttaataatgc catatcaagt tctccggatt ttaactgaag
    14341 tgctttcgca ttgtcatccg gtacgatctt gaatacaact ttatcaatgg aaggctcacc
    14401 cttaaaatag tcttcgttct taaccagggt gatcacctgt ccctcatccc aggattcaat
    14461 cttgtatgga cctgttccta ccggattacg gaagaagtca gaagtctgca tatcttctcc
    14521 ctcaagcaga tgcttaggaa gaaccgccat tgtcatatag tcaaggaatg caacgttttt
    14581 gtcctcaagc ttgaacgcaa ccgtatgatc atcaataaca ttgatctctt ctacgtcctc
    14641 gtagttgggt gcattctcag aaccgttttc cggatccatg atcgcttcaa tagtaaattt
    14701 aacatcctct gctgtcacag gttcgccgtc ctgccacttg gcatcttctg ccatatggaa
    14761 tgtataagta ttggtttcat catcaaaatc ccagctctct gccagtcccg gaactacctg
    14821 attatctccg tcatgtgcag ttaaaccgtt gaagatcaga atattgatct ctccatgttc
    14881 atccatagca ggattgatcc tggtataatc tccgcttccg tataccagtg ttgtttcttc
    14941 ctctgccagt acaggcactg ttgttccggc actgaccagt gatacagtca gaagcactcc
    15001 ggccattgtc aataaaccct ttttcatttc atgttccctc gctttttctt tattggtaac
    15061 tggcttttat gatatcagac aattctgaca tcagtaagcc ctgcgggggg atattgacat
    15121 attccttttt gtgccttata tatgccatat atggcatacc tgcagcagta tcattactct
    15181 ccatcgcagt gcgatcctgc ccggagtgtt atttatgtta taggaacatt acagagtaat
    15241 ttcctataac ataaataaca ctccgccttt agtatttcac taaaagcgaa gtgtcctgat
    15301 attaatataa ttttgcacca gccggaatat ggtcatctac catcagaaga tgaagttttt
    15361 cttcgccctc ttcctcatgg attgcactaa gtaacattcc acaagattca attcccatca
    15421 ttgctctagg tggcagattt gtgatagcta taagtgtctt acctaccagt tcttctggtt
    15481 cgtagaatgc gtgaataccg cttaaaatgg tacgttctac gcctgttcca tcatccagag
    15541 taaactgtaa aagtttcttt gacttcggca ctgcgacaca ctcttttacc tttactgcac
    15601 ggaaatcgga cttgctgaaa gtctcaaaat ctacctcatc tttgaacaat ggttcgattt
    15661 ctacctttga aaaatcgatc ttctcaacgg gcttttctga aaccgtttta gcctgcgcaa
    15721 cgttgtttgc ttcgttctta gcagcgccct gggacttcat tgtcgggaac agcagtacgt
    15781 cacggattgc ctggctgtct gtaagcagca taaccagacg gtcgattccg tatccgatac
    15841 cacctgtagg cggcatacca atctccagtg cattcaggaa gtcttcatct gtgtggtttg
    15901 cttcctcatc tcctgcgtca gcaagagcat cctgtgcttt gaaacgctct ctctggtcaa
    15961 tcgggtcatt gagctcggaa tatgcgttac acatttccca tgtattaatg aacagctcga
    16021 aacgctctac cttattcgga tcagaaggtt tcttctttgt cagcggagaa atctcgatcg
    16081 gatgatccat gataaatgtt ggctggatca gttctttttc acagtattcc tcgaaaaaca
    16141 ggttgatgat atcacccttc ttatgacgtt cttcatattc aatatgatgc tcatctgcaa
    16201 gcttcttagc tgcttcatca tctgcaactt catcaaagtc aattcctgca tatttcttga
    16261 tcgcatcatt catggtcaga cgttcaaaag gtttgctgag gtcgatctcg ataccattgt
    16321 aggaaatctt cgcagaacca caaaccttct ctgccagata acggaacata gactcagtca
    16381 gttccatcat gccttcataa tctgtatatg cctgataaag ctccatcaga gtaaactccg
    16441 gattatgacg ggtatctaca ccttcattac ggaatacacg tccgatctca taaactctct
    16501 caagaccacc aacgatcagt ctcttcagat aaagttcaag agaaatacga agcttaacat
    16561 cttcgttcag tgcattataa tgagtttcaa acggtcttgc tgcagcaccg cctgcatttg
    16621 caaccagcat cggagtttcc acttccatga agtcacggtc tgcaaggaag ttgcggattt
    16681 ctttcagaat ctgggaacgt ttgataaata cgttcttgct gtcctgattc atgataaggt
    16741 ctacatatct ctgacgataa cgcatatctg tatctgtcag tccatggaat ttctcaggaa
    16801 gaatctgtaa gctcttggaa agaagagtca tgctttctgc atggatagaa atctcacctg
    16861 ttcttgttct gaatgcaaaa ccttccagtc cgaagatatc accgatatcg gatttcttaa
    16921 aatctgcata agaatctgct ccgatcgcat ctcttgctac atatacctga atatttccct
    16981 gtaaatcctg gatattgcag aaagaagcct tacccataac tctcttgaac atcatacggc
    17041 ctgcgatcgc tacgtggatc gggcttgcgt ccatgatttc tcttctctct tcgtaatctt
    17101 tcttaacagc ttcctttgcc tgttcttcat caagacctgt tacatctact tctgcacgat
    17161 ctttgagaag ttctgcctca tgtgcttcat aaagactctt cacttccatt gagtgatgag
    17221 tctggttaaa ctttgtgatc tgaaatgggt cttttccatt agcctgaaga tctgccagtt
    17281 tatcgcgacg taccttcagt aactggttta aatcctgttc ctgcttctgc tgtgccacct
    17341 tggttcctcc ttatatactt gtataattat tatttgttca taactgttca gtaccttcac
    17401 agttacgagc caaaatgcat taaaaatcac ctgcggtgat ggcattttgg cttgtatgtc
    17461 tcgggatttt ggtacttacg tgccaaaaca cctcgcggga tagtagacta ctgaatagtt
    17521 acatttattc tacatttcca tgtgatatca gatgtaacag aaaattccat tcaacagaat
    17581 cctgtatttt caaatatcaa tttatcagat atacagacaa aaataagaaa taacaaactt
    17641 cttattgtct tcttattatc tgcttatata gtcagagaaa ggtgcgcgtt atgcctttgc
    17701 atgataaatc tctacgcgca cctcagtcat ctgtctctta tacgtttctc tgaatctcca
    17761 gaactttgta tttcatgatt cctgccggca tctctacttc tactacatcg cctgtatgcg
    17821 caccgataag tgcttttcct acgggagatt cgttggagat tttgccttca aggctgttag
    17881 cctctgtgga acctacgatc ttcagctcga tgtcttcttc gaattcaaaa tcgtgtactt
    17941 taaccttaca tccaacgctg atcttgtcca gatctacttc gtcctctaca acaacttcag
    18001 cattctttaa gatcttctcg atttcttcga tacgtgcttc aatgtctctc tgttcgtctt
    18061 tcgctgcatc gtattccgca ttctcagata agtcgccctg ttctctggct tccttaattt
    18121 tctctgctac ttcttttctc tttactacct ttaaatcatg cagctcttct tctaattttt
    18181 taagtcctgc ataagttagt aagtttttct tttcttccat aataacctta ctccctttca
    18241 acatccggta tagtcttctg cgtttctttt cgccccggat cactacatgg atttatgtgc
    18301 ttcctgatac atcatacctg aaaaagggca gacttattct aggatattca caatttctgt
    18361 attatacaaa aaagcacccg gattgtcaag cattccgagc gcctttgcta ttgtttatat
    18421 ttctcatttc acattgtttt aacttttatt ataatcttga taaaagcgcc tccagagctt
    18481 cataactttc tatggtgttt gactctcttc tgagccctgc gctgtgacgc atacctgcag
    18541 tataccaggc aacgtgttta cgcatctcac ggattcctac gaactcccct tttaattcaa
    18601 tctgttttct ggcatgacgc aggatcattt cacgtatttc ctctacagac ggacgttcca
    18661 acagttcccc tgtccggaaa taatggttca gttcgcggaa caaccaggga tttcccctta
    18721 ctgcacgtcc caccataaca gcatcacatc cggtctgttt taccagtgct tctgctttca
    18781 gcggagaatc tacatctccg tttccaatta caggaatggt aatcgcttct tttaccctgc
    18841 ggatcgtatc ccagtctgca gtgccggaat agtactgctg tctggtgcgc ccatgcactg
    18901 caagtgctgc tgctcccgca tcctcaatac gtttcgcgat ctcaacaata tctaccggtt
    18961 cattctcaaa tccgattcgc accttgacag tcaacggttt actgatcgca gatgatactt
    19021 ttcgaacgat ctcttcaatc agattcgggt tcttcaataa tgcagatccc tcaccgttat
    19081 tgacaacctt cggcacaggg catcccatat tgatgtccag gatatcgaaa tctctgtcct
    19141 caatggattt cgcaacctct gccataagat ccggttcact gccaaaaagc tgcatggaaa
    19201 ccgggtgctc acatggatca atctccatca gggcaatggt gttcttattg tgaaaagaaa
    19261 tcgcttttgc actgatcatt tccatacaga tcagtccggc cccctgctct ttacagagcg
    19321 ttcgaaatgg caggtctgtg acacctgcca ttggtgcaag aacaaacgga ttatcaatct
    19381 gtacgtttcc tattttccac tgcatctcca tgaatggttc tcttgcccgt accggctcat
    19441 tccttgtccg catggattca gtcctcactg cccgatgttt tcttcagttc ttcttccggg
    19501 atgtctccga gaaagaatac tttataatac atttccagag attccagaag ttctttcttg
    19561 tcctgctgaa gctgtttgat cttcagtccg cttccgatct catcgtagcg ggcatctgct
    19621 tccagagctt ctgttttaaa ctgaagtctg ccgttttcgt caaattccag agtcaggatt
    19681 cctcctacat cctctgtgta aagaacacac atgatctgaa gttctttctt cacatgatcc
    19741 ggaagtgcat tgaaatcctg attaaaataa aatttcttct cataggcgct tgcgccgcag
    19801 agagtaatgt tgtcctgata cataaattac tgtttccctc tttcgaaatt attcagaatc
    19861 tttatacata gctgtaaagt ccacgcaccg aaacctctcc tgcgctgact ttcacaactt
    19921 ctttattctg cctttccaca agcagttcac ctttttcatc aatccccctg cagattcctt
    19981 cgaaacttct gtcaccatcg atcactctga ccggctggtt cagatttgca aggattctgt
    20041 gataatcatc cagcagatga gtaaaatcac aggtctggat aaatttctca tagttgatct
    20101 cgaaatattt catcaccagt gcaatcacct gatttctgtc atattcatga ccgccctcca
    20161 gaatcagtga agtcgctttg tcctgcattt cttccggaaa ttctttcagg tttacattga
    20221 ttcctactcc gatgagaaca taattgatct tcacaccatt tgttcccatc tcggtcagga
    20281 taccacagat tttcttctta gagatcacaa tatcattggg ccatttaata cttacgttaa
    20341 accccagttc ctctgctgcc tgtgccactg ccattcccat aaccagtgtc agcatagacg
    20401 catactgcgg tgaaaattcc ggacgtaaga gcagtgtcat cataatagag ctgccctccg
    20461 gcgcttccca tcttctgtca aaacgccctc ttcccgcaga ctgaaattcc gcaactgcca
    20521 gagttccatg ctctgcacca tcttttgcca gacgcttgat ccatgaattg gtagaatctg
    20581 tttcttttgc aaaatggacg gttttgcccg cccattttgt gtctatcgcc tgtgaaattg
    20641 tttcttcatt atacagatgt gccattattt gttctccggc tctcctaatg ttgctaccat
    20701 aacagctttg atggtatgca tacggttctc tgcctcatca aatactttgg actgctcaga
    20761 ttcaaatact tcgtctgtta cttccatgtc tgtcagattg aatctctcgc ccatttcctt
    20821 gccgatcttt gtcttaaggt catggaatgc aggcagacag tgaaggaaga ttgctttctc
    20881 tcccgcgttc ttcataacgt cctttgttac tttataaggt gtcagatcat ggattctctc
    20941 tgtccatacc tcatccggct cacccatgga tacccatacg tctgtataga taacgtctgc
    21001 atcctttgtt ccttcctgaa catcctctgt cagagtgatg cttccgccgg atgcttttgc
    21061 atattcctcg cactgagcaa ctaactctgc attcgggaaa tatttcttag ttgtgcaggc
    21121 tacgaaatgc atgccaagtt ttgtgcaggc aatcatcagg gaattaccca tattgtaacg
    21181 ggcatcaccc atgtatacaa gcttacgtcc ttttaaatct ccgaaatgct cacggattgt
    21241 cagcatatca gcgatcatct gtgtcggatg atattcgttc gtaaggccat tccataccgg
    21301 aacacctgca tgttttgcaa gctcttctac gacttcctga ccaaatccac gatattcgat
    21361 accatcatac attcttccca gaactcttgc tgtatctgca atgctttctt tcttaccgat
    21421 ctgagaacct gtcggatcca agtatgtgct tcccattcca agatcatggg cagctacctc
    21481 aaaagagcag cgggttcttg tacttgtttt ttcaaaaata agtgcaatat ttttgcctct
    21541 gtaatattcg tgaagctcgc cggctttctt ctttgcttta agttctgcgg ataaatcaat
    21601 aagatatgtg atctcctccg gtgtaaagtc tttcagtgtc agaaagtttc ttccttttaa
    21661 gttcataata attctctccc cgatgtcatt tattctcttt tgttatttgt ctgtctccgc
    21721 tttcggatca gtgacgatct ctttcagcga acttgccaga cctgcaattc cctgaatatc
    21781 cgacggaatg atgatcttgg ttgccttacc gtctgctgcc ctggcaaatg cttccagact
    21841 cttcagtgtc agaactgcct catctgctcc tgcctctctg atcatgcgga taccctctgc
    21901 attggcattc tgaactttca gaactgcctc agcctgtcct tctgcctcct tgatcatacg
    21961 ctctttctga gcctctgcgc ggaggattgc tgcctgtttc tcagcttctg catccagaat
    22021 tgcggattgt ttcttacctt ctgcaacaag gatcgtagat ttcttctgac cttctgcgat
    22081 caggattgct tcacgacgtt cacgctcagc tttcatctgt ttctccattg catcctggat
    22141 agctgccgga ggaataatgt tcttcagttc cacacgattg accttgattc cccatggatc
    22201 agtagcttca tcaagagaag ctctcatctt ggtattgatc gtctcacgtg atgtaagtgt
    22261 ctcatccagt tccatgtcac cgataatatt tcttaaggtt gttgcggaca gattctcgat
    22321 tgccatgatc ggattctcca caccgtaagt ataaagcttc ggatctgtaa tctgaaagaa
    22381 tacgacagta tcgatctgca tggttacgtt atcctttgtg ataaccggct gcggtggaaa
    22441 atccactacc tgttccttca gatttacctt tctggctact cgttcaataa acggaacctt
    22501 gaagtgaatt cctgtactcc aggtcgcctg ataagctccc agacgttcca ggattactgc
    22561 atatgcctgt ggtacaatgc ggacgcagga tgcaaggacc cataccgcaa taacgataat
    22621 aactatcaga aaaaccacgc ccataactaa tcctccttat tctgttttac gataagtttc
    22681 acacctcgga tctcttcgat cgttacaacg gtgccttcgc cgattttttc gtcatcagca
    22741 gaacgtgcag tccattcaat gtcattgata cgaacctggc ctgtctgcgc cagattgtca
    22801 atctcctgaa tcacaactgc tttctttcca atcagactgt ttgcattggt ctgtatggtt
    22861 tctttgttga agtacttcat tgccagtggt ctggtaaaga tgagaagtac caatgaaaca
    22921 cacagaaaca gcaatatctg tacagtgagg ttcgctcctg cataacatgc aatagcagcg
    22981 accagtgcgc cgccggcaaa ccagatggtg gtcagtcctg ccgtaattgc ctctacaacc
    23041 agcaataacg caagaatccc aagccagatc aaaggctgca tgaaactcct cctttcgtct
    23101 gctatcgtct ctgacgagca gcttcataca tgagaattcc ggatgccatg gcagcattca
    23161 gagattctac ttttccacac ataggaatcc gtatcagaca atcagcactg tctgccgctt
    23221 catctgtcag tccttttccc tcattgccga tcaggaaagc agttcctcct gtgtaggatt
    23281 cctgatcata agagttctta ccatgcagat gcgctgcaaa tgtgcggata tttctgtctt
    23341 tcagttcctg tgctaatgat accacacttt ccacatatag gaaaggcatt ctgtagacag
    23401 aacccatggt tgatcgtata accttcgggt ttgtgatatc cacgcaggtc tttgtcagaa
    23461 gaactccgct tactccggcg ccttctcctg tacggagaat agttcccgca tttcccggat
    23521 cctgcaggtc ttccaggatc ataaccagcg gatttttccc gccgagaata tcttccatta
    23581 tataggaagg tttcttaagc actgtcataa ttccctgcgg cgtctgcgta tcgctcatct
    23641 gtcgaaaaac agaatctgtt actatttcat atggaagttt ctccacctga gccatcagtt
    23701 ctgcatcacc gctgagagct tctgatacat acaccttcga aatccaatcc ctaggtgctt
    23761 cgccgaacat ttttcttcct tctgcaacaa aaagcccttg ttctctgcgg gcttttgttt
    23821 tctggttcag ctgtatgata ttttttacct gtccgttatt tacactggtg atcatatctt
    23881 atcctcaatt ttccgaaacc ttattcaggt cactgtttgc tcctgctgca acaactgtgc
    23941 agtctgccgg aaacacttca tccggcgcca cattcatatt catcttatcg ccatgcttca
    24001 gaccgatcag attgatgtta tacttttccc tgaccttcag ctcccgaagt gtttttccta
    24061 tccattcccg cgggatctta aattctgcca tactgaaatc agaagaaagc tcaaacatat
    24121 cgacaaatcc actggagaga agatttcttg caactctgat acccatggaa tgttcaggat
    24181 aaatcacctt atctgcccct attttctcca gtatcctgcc atgaagagga ttcattgcct
    24241 tcgccatgac aaaaggaact ccaagttctt tcacctgcat cgttgctgta atactggcct
    24301 ccatattctc ggctactgcg atgaccgcaa catctacgtt ctgaactcca agcgattcca
    24361 aaattcccgg atcacgcaca tctgccttga cagcatagga aactttctct gcgatatcct
    24421 gcacattatc atctttatcg tcaatggcaa gtacatcgca gttcgcctct gccagagtaa
    24481 gggctaccgt catcccgaac tgcccaagac cgatcactgc ataagatttg ttcttcattt
    24541 tcttcctcat ttctctttct gaacatgtat tcgttatcct ctgcgaatat ccgcctgact
    24601 gccaaaccgt tactatccaa tcataatatc ttcttctgcc agatgtgaat ctgccggcat
    24661 tttctgtaca cgtacagtca ctgcagttcc cagagtcagc ggaccgattc ttcccagata
    24721 catagtcagg atcacgatcc atttgcctgc tgtattcagg cagggtgtca gccctctgct
    24781 aagtcctacc gttgcaaccg cagatgtaat ttcataaaca acatcttcta caggtgcttc
    24841 cggcatggct gcacacaaca gcatactcat gagaataagt actgtaagta ccatacctgt
    24901 cactacgatg gcagaacgga tatagctgtc tctgattctc ctgtggtgta cttccacatc
    24961 ccgttttccc tgaagattgg atttcagact cagcagaagg accgcaactg ttgttgtctt
    25021 aattccacct gctgttccca taggggaacc gccgatcaac atcaatatca ggcacagcat
    25081 ataagttgca ttggaaaatc tctcctggct tacagtgaaa aatccggctg ttcttgtggt
    25141 aactgactgg aacaaagatg ccatccactt cgttcccggc gaatatgtcc cgatactttc
    25201 aggatttcca tgatcaaaca gaaagatcag tactgtaccg cccacaacaa gtatcagcgt
    25261 catcatcaac acgatcttac tgtgaagacg gagattttta aacacacgtc ccacaggaag
    25321 tttccctctg atgactcgtt tgatcttatc ccagatatcc caccagacca caaacccaag
    25381 acctgacata atgatcaatc ctacacttgt aaagtttacg atcggatcag caacatactg
    25441 agccaggcta tcctctccca gcagatcaat accagcatta cagaatgcag atactgccgt
    25501 aaaaacagag tatcccagac cttttgccag accgaactgc ggaataaatc gaactgcata
    25561 acacactgca ccgattcctt ctgctccaaa cacacagatc agaactttct tcaccagcct
    25621 gaccattcca cccattctgt ccatattata ggactcctgg ataacacgcc ggttcctcag
    25681 cgatattttc tttctcagac ttataaaaat aatactgccc agggaaatca gtcccacacc
    25741 tccgatctgg atcaggacaa gaataataag cttgcctgcc agagtccagt gagttgccgt
    25801 aacaactgtc acaagccctg ttacacatat acttgtggtt gcagtaaaca tggcatctgt
    25861 aaaagatgtg tggtatccgg gtgccgtaca aaacggcagc cacagaagta atccccctaa
    25921 gataatcact cccgcaaaac cgagggtaat tatctgagca gtatttaatt tatgcagtct
    25981 gttatataac tgcatctttt tattcatttg ttatttgttg tttttatcat agttatgaga
    26041 aagtgactgg catacttccc actgatagtc agagataccc ttctgcatag caagtgcttc
    26101 attgatatcg aagtctacga attcaccgtg acgatatccg acaacgcggc atgtcttgcc
    26161 tgcaataaga aggtctacag ccagagcacc catcatagat gcatatacac ggtctttaca
    26221 tgtcgggctt ccaccacgct gcatgtgtcc aaggattgta gcacgtgttt cgatacctgt
    26281 ggcagcttcg atacgttttg ccattgcttc agaatgaccg ataccttctg cattgatgat
    26341 gatgtgatgc tttttgcctt tcttacgact ctcgataatg ttattgatca gcttctgctc
    26401 atcatagtca tatttctcag gaaccagaac gtcctcagca ccgttggcaa taccgcacca
    26461 cagagcaata tatcctgcac cacgtcccat aacctcgatg atactgcatc tctcatgaga
    26521 agtggaagta tcacgtactt tatcaattgc ttccattgct gtgtttacag ctgtatcaaa
    26581 tccgatggta tattctgtac atgcaatgtc aaggtcgatt gttccaggaa gaccgattgt
    26641 attgattccg agattcgcaa gcttctgagc acctgcgaag gatccgtcac caccgatgac
    26701 aacaagtccg tcaattccat gttttctgca gatctctgct ccacgtttct ggccttcttc
    26761 tgtacggaat tctgcacaac gtgctgtata aaggattgta cctccacgct cgattgtatc
    26821 agatacatct tttgcgctca tgtcaataat ctcttcgttt aacagaccat tgtatccttt
    26881 cagaatacct tttacattta atccattgct taaacctctt ctgacaactg cacgaattgc
    26941 agcattcatt ccaggtgcgt caccaccgct ggtaaggaca ccaatggttt ttatcttgtt
    27001 ttctgccatc ttttcttcct ccatcttctg aatctatctc atttttacta tacacatatt
    27061 cgggtatagt ttagcgcatt tctataagtt tttcaatcct ctttctacaa ctttaacatt
    27121 ggttttacca tatttttcgt acatatagtt cagccatgag tcctgaattt gtgcatgata
    27181 tccaaccgga agcttcttta tggcctttac gtctttcagg tagatcacaa cagcactgtc
    27241 acccggtgaa cgtctgaggt ctgcaagcaa ttcctgagat tgctgtgtgt agctttcttt
    27301 attgtcaaac tgtatccaga tttcccttgg catattgtca aagggacgga ttttttcaag
    27361 gatcagttta ctggcgcgat cgtcctctgc agatactctg ccctgaataa atactctgcc
    27421 ttcttcattc aggaactgct gacttttctc ataatctctg ggaaacacaa ctacctccac
    27481 tgttcctacc agatcctcca ctgtgaggaa tgccataacc ttattattct ttgtatattt
    27541 gatggtttta tccgtaatca ttccaccgat aattactttc tgaccatcca tgactttcgg
    27601 aagatttgtt tcttcatcct gctgaaaatc cgaggttttc gcagaaatgg ttttctccat
    27661 catttcccgg taattttcca gaggatgacc actgaggtag attcccagaa cttccttctc
    27721 atatcccaga agttcctctt ttccgaattc ctccacatcg ggcatacgga tttcaaattc
    27781 tttcttgtcc tcctcgctta ccagatcaaa aaggctcatc tggcctgcca gtgaattctt
    27841 cttatcctga ctgatactgt ctgaaatctt ctgataaacc agcattttct ggcggcggtt
    27901 accatccagg caatccagcg cacctgcttt gataaagttt tctactgctc gcttattcat
    27961 ctgcggagag tttcgttcca taaaatcctt cagggaacga tattgtccgt tctcttccct
    28021 ctctcttacc aagctttcaa taaccggacg tcccacactc tttatagcgg acagaccata
    28081 tcggatcgca ccattatcta cagagaatcc gtacattcca cagttcacat ccggcggcag
    28141 gatcttaatc cccatctgtc tgcagaccaa aatatattct gcaaccttat tgggaaaatc
    28201 aataaccgat gtcataagtg cagccataaa ttctacagga taatagtact tcagataggc
    28261 tgtctggtag gaaacaactg cataggctgc tgcatgggac ttattaaagg catatttggc
    28321 aaaatcaatc atttcatcat agattttatt tgccgttgcc tcatcaattc catttgcaat
    28381 acagcctgga actccctctg cttcatttcc gtaaacaaag ttctggcgct ccttttccat
    28441 gacagaggcc ttctttttgg acattgcacg gcgcaccagg tcgcttcggc ccagagtata
    28501 acctgccagg ttacgaacga tctgcataac ctgttcctga tagacaatac acccataagt
    28561 aggcttcagg attggctcca gctgagggca gtcgtaacgg atggtatccg gacggttttt
    28621 gccccggata tactgaggga taaaatccat gggacccgga cggtaaagag aaattcctgc
    28681 gatcacatct tccaggctct gaggcttcag ttccttcatg aagtttttca ttcccgcact
    28741 ttcaagctga aatacaccat cagaacgacc ggtgccgagg gaatccaaaa cctttttatc
    28801 atcataattg attttctgca tgtccagagt cactcctgca tccttctgga tcagctttac
    28861 tgcattctgg atcacagtca gggtacgaag tcccaggaag tccattttca aaagtcccaa
    28921 ttcttccagt gttgtcatgg taaactgtgt gacaatggaa ccatccgacg ctctggaaag
    28981 cggtacatat tcagaaacat ctttctgact gataaccaca ccggctgcat gcatggaagt
    29041 atgtcttgga agtccctcca gacgtcttgc catatcgatc agataatgaa tttcatcctg
    29101 ctcttcatat gcttttttca gctcagggtt catagtaagg gctttatcta tagtaatatt
    29161 taattcctgc gggatcattt tcgcaatgga atccacctgt gcataaggca tatccagtac
    29221 acggcccaca tcccggatca caccacgggc tgccagcgta ccaaaagtaa cgatctgaac
    29281 cacacaatca tccccatatt tacggcggac atattcaata acctcctgcc ttctttcgaa
    29341 gcagaagtca acgtcaatat caggcataga tactcgttcc ggattcagaa atcgctcaaa
    29401 gagcagatca tagcgaatag gatcaagctg tgtgatgccc aaggtataag caaccagact
    29461 tcccgctgca gagccacgtc ccggtcctac cataatgtca tgatcgcggg catatttaat
    29521 aaaatcccat actataagga agtaatccac gtagcccata ttttttatag tagataattc
    29581 gtaattcaga cgagctttta attcctctgt aaccggctga taacgttcct ccagtccctc
    29641 gaagcatagc ttgttcagat attcccagga tgtaagacca tccggcacat cgaatcttgg
    29701 aagcttagtc acgccaaatt caatctcaac atgacatctc tgtgcaatct tatgagtatt
    29761 ctccagggcc tccaaagcat atgggaaaag ttctgccatt tcttccgggg atttcacata
    29821 atactggccg ccctcataac gcattctgtc ctcgtctgcc agtttcttgt ttgtctgcag
    29881 acagagaaga atatcatgag gatcagcatc ctccgccctt gtataatgca catcattggt
    29941 tgccacaagg tcaatccctg tttcttcgtg catacggaga agttcctgat ttaccagtct
    30001 ctgcgcagga attccatgat cctgtaattc caggaagaaa ttgccttttc caaaaatatc
    30061 ctgataacga agagctgctg cctttgcatc ctcatacatg cctctctgaa gatatctggc
    30121 aacctcacct gccagacagg cacttaatgc gataattccc tcatggtact cttttaatac
    30181 cgcaaggtct actcgaggtt tataatagaa accttctgtg aaaccctttg atactatctt
    30241 catcaggttg gcatacccct gattattttc tgccagaaga accaggtggt aatatctgtc
    30301 ttcgccgctt cctgcttcac ggtcaaatcg ggagccgggg gctacataaa cctcacatcc
    30361 cagaatcgga ttgattccct cggctcttgc tgcacgataa aaatcaatca caccatacat
    30421 aactccatga tctgtgatcg ccgcactgtc catgccaagt tctttgacac gggctacata
    30481 ttcttttatc ttattggaac catccagcag actgtattct gtatggacgt gaagatgtgt
    30541 aaactccaac tctatttctc tcctgttcta atatctgcaa ttttatcgca gcatattttt
    30601 ataaatttaa ctgaatcagg taagtcccat gcttcaattg cgaaaagcaa taatcattgg
    30661 gtggggactt gcttgtctgt tactatttcg cgtagattct cttgtcctta atctctactt
    30721 tgccttcctt catcagatgg ccaatggcac gtttaaatgc tcccttactc agaccaaatt
    30781 ctcgctggat cacttccggt gatgccttgt catcaaatgg aagaacacct gcaaattcat
    30841 tgattacttc aaggacattc tccgcatcct cattgatctg aagatatgct ttctgacgat
    30901 ctgtgacgtt catttttccg tcttctttta cttcgctgac acgcagctca aggatcttgc
    30961 ccgggcggta ttttcccttg gcttctcttg cagggatcag tgcggaatat ttatcatcaa
    31021 ctgccacaaa tacaccgaat ctgtcgctga tctcataaac acggcccttc accatatctc
    31081 ccactacata aggagttctg gtggaaagat aatgataaac cttcatagtt gcgcagagac
    31141 gactgctctt gtcaatatag agtgctacca gcacatcttc tccctctctt actttcagag
    31201 tctgttcatg atatggaagc agaaggtctt tctccagtcc ccagtcaagg aatgcaccaa
    31261 tccttgttac ctgagagact ttcagaactg ctgtctgacc tacctgaagc ttcggttctt
    31321 ttgttgttgc aataagacga tcctgagaat ccttgtagat aaatgcttca atgctgtcgc
    31381 cttcttttgt tccttccgga acctgtctgg acggaagaag aacacggttc tttgcatctg
    31441 cctgcatatc ctcacccaga tatacaccaa aatctacgga ctttactact gttaatttct
    31501 gtttttttcc taactcaatc atataatctc catttctgtt gtgatagtat ctttttaaag
    31561 aaaattaaaa atatactata ctttcatcat cctctgacat tagctttata tttcagtata
    31621 acataaataa taaaggcggc acaatgccgc cttattattt tttattatct tattttacgt
    31681 tactgttcac tatagatcag aatgtatttt attccaactc gatttcacat acactcagac
    31741 agattcttta tcgtgtatta tatattctgc atccacttgc atgccagttc tatccaggac
    31801 tggcattctt tctgtacaca gccgccgtct tcgtggcttg tctcttcgtt tgcaagactt
    31861 aaaccatgtc cgccaaccgg ataaagatgc atttctaccg gaatttcaaa tttgcgcaac
    31921 gcattgaaga acaacagaga attctctaca ggaacacaat catctgtcac tgtatgccac
    31981 aggaatgtgg ttggtgtatc tttagatacc tggaattcca gagactgttc ttttctctta
    32041 tcaagatcat tataatcctc tccaagcaag cattcaaagg aacctgtatg tgcaaactca
    32101 ccggaagtga tcaccggata acttaagatc ataccatttg gtttcaccat atcagaagtt
    32161 accccaagtg tctgtgcaat aaaatctttc ttccagaaaa cgccaagact tgctgccaga
    32221 tgaccacctg cggaaaagcc ctgcagaaca attttattcg tatcaatatg aaactctgca
    32281 gcattttttc ttaaaaaagc aacagacttc gcaagctgca agagtgccag tggatatctg
    32341 gcaggttcta cgctatagcg caggatcact gcatgatatc cccttaccag atactgcatg
    32401 gcgatcggtt ctgcttctct gtcagaagtc atagcatacc cgcctcccgg gcagatcaca
    32461 actaccggac gctttctgtt gatatgcatt tcaatggaat tatccagaaa ataagtgtat
    32521 aaatctgcag tttctttata tccgtctact gcaatttcaa tcttcttata aatcataagt
    32581 tcctcctgta tatttcatca ttttttccat ttctactccc cattccgcta atttttgggg
    32641 aatatttaca gctacttttc tcagttttgt tttgccatcc tttgctgcca gacgcttata
    32701 taaatgtata tttccatctg catcaagaac aatataaaaa cactctaaaa tatgacctga
    32761 tcctacaatt tcccgtgttg tatatatata tcccgcttta ttcttttttc ttttagaaaa
    32821 aacaatccct ctgcttctcc tgtctcggaa acttggaaaa taatcttcac gtataatttc
    32881 aagttcttcc catttataca tttttttgaa aaacagatac gatattttgc agccttcttt
    32941 acttaatgtt acgacttttg aagagaaaaa caataatggt gcatcaatca aaaactcaag
    33001 aatcaatatt ggaattcttt ctccctcttc agcagacagc cctgtccaaa taagcagtac
    33061 cgtaataagt attaccatgg ttatctgcaa aaaatctctg atctttatta cttcttgctg
    33121 atattctttt aaatttgtac agtctgctcc tttgctttga attttttttc tttgttgcct
    33181 cctcaattct atcatttcat catattgtct ttgtttttct tcttctttta ttccctctcc
    33241 tttttctgcc tccactcccc attccttcat tttttcacga acatttacgt acatttttct
    33301 aataacttgc ttttttcctt tttctaattt ataagaaaaa acattgccat gttcatcaaa
    33361 agtaatgtaa aaacaatctg aaagcggaaa cgcatcaacg atcatcctgg tagtatattt
    33421 ctttttcttc ttatttactg catatttgga aaatacaatc ccctgataac gtatgctccc
    33481 tcctttacca tatgtatacc agacatcctc tctgataaca gcaagctctt cccatttata
    33541 atttctcttc caaaacagcc aggataccgt acagccttct ctgctcagca caacagttct
    33601 catataatac aagattaaca gcaacattaa tatttccaca ctaaaaatta taaattcata
    33661 tccctctgtt cttatttcct ttctttccag gaaaatcatc agcattccta tgccgaatac
    33721 aaacgctgat ataaataata tcagtattat atgtggatct aatactctta ttttttcctg
    33781 ctgctctttc atatgcattt cctccggttg tctgaaatcc ttactgttac tattccggta
    33841 tatcaaccta atttcttgca ttttcttatg agttcattat aaggtaaata tatgtaattg
    33901 ttaaggtgca aaataaaatg ttgctgtccc gtttactgca aattaaacga accatacaat
    33961 attaaagttc agctgttatt atctttcaag gatcatgaca tcttttgcat tcatgctaac
    34021 ggtctgccct gaaacgttct cctccttcga taaagttgca gaaaattgcc cggactttct
    34081 gcaatatggc agaagaattt tccttgtttc ttctgctaca aaaaatctcc cggataagag
    34141 aagcgcttac tcttatccgg gagatttttt atatctttaa caagttcata tcttttattt
    34201 tcagatcagc aattatttat ttccctgtcc ataatatgca tttgcaccat gttttctgaa
    34261 gtagtgctta tcctgcagat cctgtggtgc aggttttacc tgtggattga tcagctcaca
    34321 gcgggatgcc atcttggcaa cttcctcaag aacaactgca ttatgtactg cttcatgagc
    34381 atccttgccc catgcaaatg gtccatggtt tttgcaaagt actgcaggta ctgcttcata
    34441 atctttgtcc ttaaacaggt ctacgatcag aagtcctgtg tttttctcat attcttcaaa
    34501 ttccttgcct tccaggcagc gtgcacatgg aacttcacca taaatataat ctgcatgtgt
    34561 ggttccataa catgggattg atcttccggc ctgtgcccag cttgttgccc atgaagaatg
    34621 tgtatgtaca actcctccga tattcgggaa tgctttgtac agttctacat gagttgctgt
    34681 atcagaagat ggattgtatt ttccttctac cttatttcca ttcaggtcta caacaaccat
    34741 atcatccggt gtcagcagat cataatcaac cccacttggt ttgatgacaa aaagtcctga
    34801 ttctctgtcg attccgctta catttcccca tgtaaatgta acaagtccgt attttggaag
    34861 atccatgttt gctttgtaaa cttcctcttt taatttctcc agcatgattc gtacctctcc
    34921 ttttgctttt cagatattct tttacaacag ctttcagatg ttccggcttt tttctgcata
    34981 tcccacccgg aaaatctctt aatatctgta ttatagcact tgtacggtac atgtaccaac
    35041 ttgtatgttg attattttac tgtttctcat acaaaaactc ttcaggcaga cttaccttga
    35101 aatcttttgc aagtgaacgg aaattgtccg gtgtaatagt ctgaagttta tttggagtca
    35161 tagaaagaat cttcaccaga atctctgcag atttttctac cgtatggagc agaccaaatg
    35221 tcaggtcaaa atcttcacct gagcagaaca ttccatgatg tgcccagata acaacatcat
    35281 atttcttcat taattctgct gtttttatgg caatttctct tccacccggt accatccagc
    35341 cgacaacacc tacgccgtcc gggaatacaa ccggacactc tgtagcagat tcccataact
    35401 ctcttgtaaa gatcttatca tccagtggca gaacaaatgt cagagcaatt gtatttgtcg
    35461 tatgtgcatg ataaataaca cgatgttttc cgtttgtcag tttcttcttc acttcatgat
    35521 tcatcagatg agtcggaagc tcactggtag gtctgccgcc ctcaaccagg ccccagcgga
    35581 ttctgtaatt tgttcctgtc tcatccagtt caatgatcgc aagacatact tccggatcca
    35641 cgataatatt tctgaaatat ttaccgcttc ccgttacaag gaaaaactct cctgctaatc
    35701 ccggaacctc taccccgatc ggtatccatt ctccaggtgt attcagacgg ggacggatca
    35761 tctctacttc ctccggtttc agacgatagc ttaagtttcc gccgtttctc tcatgccatc
    35821 cctgaagata accgtcattt gccattttga taaatccctg tacaaattta gattccagaa
    35881 ttttcataat atatataccc tccgcttttt gtggttttat gttgtttgtt tttaacaata
    35941 tattgtttct tgttgtttat gaatgtatta tatattgttt cttgttgttt gtaaataatt
    36001 tattcaaaaa atctctcaca aaaccacaca tcaattccga aatatctttg tcaattctgt
    36061 cctgctattc atcctgttca gaagattttt accgttattt ttgctgaaat tctataattg
    36121 cgtatgcttt tccgtctgta tcatcaagcg atacaatcac ctttcctgtt tctgcttttg
    36181 ctttcgggca gattatatct ccaagttttg cctgcatctt atactctgca cgcatctggt
    36241 atactttgaa atcttccggc aggaaatctt ctgccataca gatatactga cagttgttta
    36301 catgatgatt agtgtcaaga tgatgcttct gcactgtgaa tgattcctgt gaagtcatgt
    36361 catccggaag tttgattttc ctcggtgcat aatccatttc cagcttttcg ccgagtatgt
    36421 aattgtctgt atctgcaggt gtcagtctca ccggaatccc tgtactgatg gccagatgtg
    36481 tccagttgga gttcgcccag gcaagcttct tcccatccat tgtttctatg gtgaaattac
    36541 gcatacccat gaatcctttg aaatcataag gtgctgtcgt gatcctgatt tccgttccca
    36601 tagcaggata ttcatccaca attacctgcc aggaagaaag aacccacgct ctgtctcttg
    36661 ccttcagcac gtctcctcca agcccgaccg actccgcatg aaatgtacag caatcctgaa
    36721 aataattcaa aacacccggt aatgtcagtt tgccgttctc acctgtctca ctgtatctga
    36781 ctctgctgtt aaaactgtaa ttcatttttt atgtcccctt ttcttatgtc gctatgattt
    36841 tttaatatac ttcttttcta ttattataac aagccaaaaa tataaggcat aaaacagttt
    36901 ttataaactg gcctatgcct tatctttgtt ctacgttata ttatttactg taattctttg
    36961 ctacaaatgt gatcatcacc ttaaacagat ccccatccag gtatagctta aactcacctc
    37021 cctgcagctc tgtcagactc tttgcgatag agagtcccag tccgcttcct tctgtatttc
    37081 tcgccacatc tcctctgata aatcgttctg tcagttcatc cgcagagata ttcaaaggct
    37141 gtgctgaaat gtttttcagg gaaaaggtta cttttttatt tctgttgctg atctctgcat
    37201 aaactctggt gccttccatt gcgtacttca caacattgcc gaatacattt tccagaacac
    37261 gccacattct ctgaccgtct gcgtagataa tggacggttc gtctgtgaaa tgtaccatca
    37321 tagtcaggtt cttctcctgg aatttctctt caaattctcc gatcacctgc tgcaccattt
    37381 ctacaaaatc aatatcattc atctccagct tgatgtttcc ggtgcttgcc ttggatgcct
    37441 ctaccacatc ctctgtgagc accttcagtc tctgagattt ctcatccagg atgcgtaaat
    37501 attcctgtat tttcggatct gtcggatttt cccgtttcat cagatctaca taattgataa
    37561 tggaagtcag aggtgttttc aggtcatggg aaacatttgt gatcagctct gtctgcatcc
    37621 tttctttttt caggctgttc tccacagcag catccagacc actgccaata ttgttgatat
    37681 attctgccat caccttctgt tttccggtaa gtgtatctgt tttgatctta tattgcagtt
    37741 ctccatcgct tatttttttc agaccatcca taataagatc cagtccgtct gcttttctga
    37801 tggcgaaaat cataacagcc acatctacag ccatcaatgc cagcaggaat actcctgcac
    37861 cactgaatac gcagccgatg atcaggaact gcaaaaataa aaatgcacct cccacaagca
    37921 gaacttttat tttttctgct gtatttctgg aaaaagctct ggcaaaatcg gcaagcttcc
    37981 cactgcattt tccaatccat ttcagaactt ttctaatcag actgtttttc cataaggttc
    38041 ctgcctttat tcttcttacc aggctgagat atccgatcag aaaccatgcc attgtataag
    38101 tcccacatat gagacagata acaatcactg tcactactac atgactgtaa cccaatgatg
    38161 agttggcgat caaggttccc gatatgatcg ttcctgccag ccatataccg attactgttc
    38221 ctgccgcaat ttctgtatac catctgtcaa atccattcag atgtatttct tcatcctctg
    38281 gtctccttcc tgctgtcaca gtcagccaca ccataccgat cagccagagc acacttccga
    38341 agattgctcc tgccagcatc ggaaacataa gctttgaata tgtctcatag ttctctgcct
    38401 catcagccat ggaatctgcc acagaaaatt tcgtatcaac cgaaactgca aatacaaaat
    38461 cctttgtgtc aaagctctga tcaatggtat gattccacac ctgcagatct gcacccggaa
    38521 tatttgtaac acactcactt aattccgggt aaacaactgc atatgctttt tccttaaata
    38581 ttttctcaag gttctgctcc agctgtgcgt aactgctgta cgctttttta tttgtataga
    38641 ttgtctgtgt ttctttatct gcgaaaagat atgtgaggtt ggtactccct tcggtatagt
    38701 ctcccacgct gtgaaagacg gatgccagtg atgtttcata atcattgaac tgttcatctg
    38761 actgtatggc acggatgcag tccagaagtg tgaataactc cttatatgca tcctccagtc
    38821 tcccattcca ttccgtgctt ttgtttaccg catccaggat attctcagct ccctgtggtg
    38881 caaatttctc gtccagctga ctcagttcta cgaaaaaaca tcccggatat tctgtgtttg
    38941 ttctggtatc cgtaacgttg ccgatattct ctgattgtga ggtatatctg taataaagtt
    39001 catctgctac ggtatccagt ttctcggact cggtctttga ttcgaaatca tcttcttcaa
    39061 gaaaatctgt attgtaatta attttcagtg ttccatctgc caccatcttt ttaaagtcag
    39121 tccgataaaa atagtggctg gttccgtcag aagattcaca tcttatgacc tgtgaatctt
    39181 cgtcataaac accttcataa tagtttgcct cccagtcttt tccccactcc agaagatcac
    39241 ttacagagta cgcaagcccg gaagtatttt tcagagaaag gtctctgatg gaatcacggg
    39301 tgtttccttt ttcctcaaat tctgccagat caatcactgc cgatgaacca gcatttttca
    39361 gatagttaat gtcatcctgc gctgccagac tgaccagaat atctgatcct ctgttgctga
    39421 cctgtttctc aaatgcttct gattctgtgt aacttttgcc tctgtcatcc aggcggattc
    39481 cagtctgata gatcatcaca aacatagctg cacagactgc tgctgcaact gctgccagat
    39541 gcgcgatcac gatccagatt gccttgacag aactgcttct gtatcctttt cccttcataa
    39601 tgtttgtccc tttccttttt tatttaattt attactttgt caagcggata ttcgcaatcc
    39661 gctttgctac ttgctcataa ccaaataact gcttcgcagt ttatcagaat gcgcttgcac
    39721 gcctcctgat acaagcaagt ccccacccac tgcttattgc ttttcgcaat tgaagcaggg
    39781 gacttacttg atttggttaa attttttcca ccttatatcc aagcccccac acaaccttca
    39841 ggtatcttgg ctcccttgga ttaatctcaa ttttttctct gatatgccgg atatgcactg
    39901 ccactgtatt atctgctgca actgcttcct cattccagat gttttcgtag atctgattaa
    39961 tggaaaatac ccttccctga ttcttcatca gaagaaacag gatattgtac tcaataggtg
    40021 tcagcttcac cacatcaccg tccaccctga cttccttcag gtcatcgttt atgctcagac
    40081 ctcctgtctc gtagatatga gcattctcca caggtattgc tgctcccagc cttgtatatc
    40141 ttcgaagctg tgatttcacc cttgccacca gttccagggg attaaagggc ttggttacat
    40201 aatcatctgc ccctacattt aatccaagga tcttatctgc atcctctgat ttcgcagaaa
    40261 gaataatgat cggaagagga ttctcttccc ttatcttcaa agtagcccgg atgccatcca
    40321 gcttcggcat cataatatct atgatcagca gatccaccga ctgactgcgc aatgcgttaa
    40381 tcgcctccac accgtcgtat gccttcagga ccgtatagcc ttcctgctcc agataaatac
    40441 tgattgcctc tacaatgtct ttatcatcat cacagaccag aatattagcc actttacaca
    40501 cctccctcat catccgaaat gcctgtctga aatctctcat gtacagacat cc
//
LOCUS       DBMDXG010000002        21097 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_793_length_21097_cov_2.191703, whole genome shotgun sequence.
ACCESSION   DBMDXG010000002 DBMDXG010000000
VERSION     DBMDXG010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 21097)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 21097)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21097
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_793_length_21097_cov_2.191703"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="ACZPD3_00165"
     CDS             complement(<1..590)
                     /locus_tag="ACZPD3_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1414466.1"
                     /translation="MLYENIKKLVQYGVETGLTPACEKNYTINLLLDVFKEDEYVEPE
                     EEYRDIDLEEVLNALLDEAVKRNLIEDSVVYRDLFDTRLMNCLMPRPAQVQNEFWSRY
                     EKDPQEATDYFYKLSQDSDYIRRYRVKKDQKWTVDSEYGKIDITINLSKPEKDPKAIA
                     AAKLVKSSSYPKCLLCPENEGYAGRVNHPARENHRI"
     gene            complement(804..3257)
                     /locus_tag="ACZPD3_00170"
     CDS             complement(804..3257)
                     /locus_tag="ACZPD3_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005952398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414467.1"
                     /translation="MKRILKKAGILLLVFLLGTAGTALLLNSESTDNRSDFNDAVFPE
                     VMVDMNDTLINRMYGYAQPMQADFTRDSVTPLDTSKELTFKVNPYDSEVKSFSYEIRT
                     SDGSKVLENKKIKNLVKEDQYLSVDVEIGSDLRMNQEYSMQIALELDEGTAYYYTRVV
                     SRSQVHASDYAAFVKYFYEACLDKESADALGSYLEPQTTGAATNYSGININSSLSEIS
                     WGNLAPQLCQEGIPVIKEINETTASVVLEYQLTSQNEDEETELYDVKEFYRMKYQDTR
                     IYLLDFQRSANQVFDGTLPVYEDDGIILGVRDKNVEYMMNDAATVIAFVQEGDLWSYS
                     PGNEKVNQVFSFRKLKDGDFRDSRTQHDIKIVRVTDEGDIDFVLYGYMNRGSHEGYEG
                     IAVYHYNRDKNVAEERAFIPVSESFEFLKKDLEKLSYVNEKNELFLILAKNLYRINIE
                     ENSSEILEKGIKNANFVSSDNNDHAAWLVSEGDEKGNIKEIDFDTCKTRLIAPQKGQR
                     LRTVGFMNEDLIYGMLNKEDILTDEEGHKSVGIRILRIEDFEGNVKKEYRKDGLYITD
                     ISVGNTLIEFELSAKSGETSYVAQKKDTIMNNKKAAANTVKIELISASRTGVRVKLVF
                     NTTKQTDSPLTMYAKVSSSDRKDIVLDTQIPQETAYYVYGQGGLDGIYTDPAKAVLRA
                     DTLGGVVLNRTQQYVWERGNKKTKMQIDTEGIPESVLQGTYDIKTLKKSLKKTGTVID
                     LSGCSLDSVLYEISAQRPVIAKTGADTSVVIVGYDEYNTWLYDPVKKETYPYGMNDST
                     DLFQKAGNVFITYIETVNY"
     gene            complement(3254..4417)
                     /locus_tag="ACZPD3_00175"
     CDS             complement(3254..4417)
                     /locus_tag="ACZPD3_00175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1414468.1"
                     /translation="MKNLREYLEEQINENLIQAVLSAGRNKDGISKIKIRPIRLKGQI
                     CYQASATEGQKVLHKNYGRTELIEYVEKELAENFRQFQAQGAVTDGVVLVSKKGKMTI
                     KQKHHEQKEKVQIQAHNRVKQYILKEGVPVPFLIDLGVMNEQGKIIHARYDKFRQINR
                     FLEFIEDILPRLSRDREITILDFGCGKSYLTFAMYYYLRELKGYDVNIIGLDLKTDVI
                     EKCNSLALRYGYEKLHFYHGDIADYEGVSCVDMVVTLHACDTATDYALAKAVEWGAEV
                     ILSVPCCQHEVNKQIKNEMLEPVLRYGILKERMSALITDAVRADLLESKGYDTQILEF
                     IDMEHTPKNLLIRAVRTGKRSDQGKVEKMLAALNIHPTLDRLLNEKEQEGSVR"
     gene            complement(4505..4873)
                     /locus_tag="ACZPD3_00180"
     CDS             complement(4505..4873)
                     /locus_tag="ACZPD3_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414469.1"
                     /translation="MYLKEYFPFYTTYANPLLYEGERMQDKEFNLMKSYYPGTVQHIQ
                     EKIEEECDLMDYEGSRLYDEYPDKYMIYHLSCKIRESMEPEISTQAIREDFLDELIQV
                     MLCQEISRRRCRRYRCRRYF"
     gene            complement(4983..5681)
                     /locus_tag="ACZPD3_00185"
     CDS             complement(4983..5681)
                     /locus_tag="ACZPD3_00185"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HIR1414470.1"
                     /translation="MKCIGIIGAMEQEVAKIKEKMQDVTITSRARMDFYEGTLEGKKV
                     VVVRSGIGKVNAGMCTQILADVFGVEAVINTGIAGSLNNDVNIGDIVLSTDVLHHDMD
                     AMGFGYKKGQIPQMDEFSFPADEKLRKLAAKVCKEVNPEISVFEGRICSGDQFISDKS
                     VKDAIISEFGGFAVEMEGAAIGQAAYLNHIPFLVVRAISDKADGSAHMDYAEFEMAAI
                     EHSVKLTVRMIQEL"
     gene            6050..6295
                     /locus_tag="ACZPD3_00190"
     CDS             6050..6295
                     /locus_tag="ACZPD3_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR1414471.1"
                     /translation="MTYKTKGVCASHIEFEVDDNKKVHNVRFIGGCSGNTQGVASLVE
                     GMDAEEVISRLKGIKCGFKNTSCPDQLSTALEEVLNK"
     gene            complement(6445..8061)
                     /locus_tag="ACZPD3_00195"
     CDS             complement(6445..8061)
                     /locus_tag="ACZPD3_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR1414472.1"
                     /translation="MKITFIGATHEVTGSCYYLEAAGHKFLVDCGMEQGPDYYENAEI
                     PVALGEIEFVLLTHAHIDHSGNLPAIYAKGFRGPVYATDATSHLCDIMLRDSAHIQMF
                     EAEWRNRKGRRQGKPEFVPAYTMEDAMGVIRNFVGCPYNKMITPAEGISARFIDAGHL
                     LGSASIELTIREEDTEKKIVFSGDIGNTCQPLIKDPEYLHHADYIVMESTYGDRSHGE
                     KPDYVKLLSEIIQETFDRGGNLVIPSFAVGRTQEMLYFIRQIKADGLVYGHDGFKVYV
                     DSPLANEATTIFSEHQYDCFDEEAMELIQKGINPISFPGLKVSVTSDDSKSINYDEEP
                     KVIISASGMCDAGRIKHHLKHNLWNEKNTILFVGYQAVGTLGRALIEGAADVKLFGEP
                     VHVAAHICQMPGISGHADVNGLLDWAKAFEEKPQKVFVTHGDDTVTEIFAKRLTDELG
                     YDTMAPFSGTVYDLADDVCLYEAKGVKIQKASASPKATRAARAFEKLLALGYRLITVI
                     RKNEGTPNKDLERFSRDVQSLCDKWDRTDM"
     gene            complement(8632..9072)
                     /locus_tag="ACZPD3_00200"
     CDS             complement(8632..9072)
                     /locus_tag="ACZPD3_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414473.1"
                     /translation="MKNKTDIQTKNQQIDKILQEQYDVETKILESALLNVAGKTSWDE
                     FPEESEEKIRAGYDRLITRLRETGDFNEAARAEKTGSTGAAKEKLESLEYDGNRTHRI
                     LKSAAAAVAVVMLTALVGMTAIADYRYPDKNTQTYQKTSVFEKK"
     gene            9241..10311
                     /locus_tag="ACZPD3_00205"
     CDS             9241..10311
                     /locus_tag="ACZPD3_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR1414474.1"
                     /translation="MKHIVLTGGGTAGHVTPNIALIPRLKELGYEISYIGSYDGIEKK
                     LIEEMNIPYYGISSGKLRRYFDLKNFTDPFRVLKGFGEAKKLLKQLKPDVVFSKGGFV
                     TVPVVIAAGRRKIPTIIHESDMTPGLANKICIPSATKVCCNFPETVKSLPADKAVLTG
                     TPIRQELLNGSKEAAREFCGFTDDKPVLMVIGGSLGAASVNENIRKILPELLKEFQVI
                     HLCGKGKMDESLKDTKGYVQYEYIKQELADLFALSDIVISRAGANAICELNALKKPNL
                     LIPLSANASRGDQILNARSFERQGFSMVLEEEEITESTLLNAIRELYQNRESYVHAMS
                     ESSHMNSIEKITGLIEDCVNKQ"
     gene            complement(10312..10845)
                     /locus_tag="ACZPD3_00210"
     CDS             complement(10312..10845)
                     /locus_tag="ACZPD3_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016430.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1414475.1"
                     /translation="MENIDFVSIYRKYRSRAFNIGKFILGDEDEAEDVCQDVFETLFH
                     MGRSVDFSDEKKLENLIVRISYHKASDHRKKAFRKYEYANSDAILEMTDMRVRKGNRV
                     DEIVLNLEAAGYLQSIFEKLREKNRTNYEIYVSVTLYDIPTRLVARHYHITENNVNNR
                     VMRTRRWLAREYKKITR"
     gene            complement(10872..11285)
                     /locus_tag="ACZPD3_00215"
     CDS             complement(10872..11285)
                     /locus_tag="ACZPD3_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR1414476.1"
                     /translation="MENCIFCKIANGEIPAATLYEDENFRVILDLGPASKGHALILPK
                     SHAANIYELSDEMAAKAMILAKKMATAMTAALKCDGFNIVQNNGECAGQTVFHFHMHL
                     IPRYKGDQVGITWHPGELSDADKEEILLKVKEQLS"
     gene            11425..12108
                     /locus_tag="ACZPD3_00220"
     CDS             11425..12108
                     /locus_tag="ACZPD3_00220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414477.1"
                     /translation="MQIEITKQSEKDFRFLLSRLSHEIRNPVTLINSELQLMASSHPE
                     LCSYRQWDSLMDNLEYVKELLKELSDFSHAQTVTLVPVNPAEFLTTVLSCQKNTLDYL
                     GIRLEIHVEHTSSPVFLDRIKMRQVIFNLIKNSCEAIDQPGGKIIVNLTPADSGICIC
                     IKDNGCGMTHKQTENIFHPFITYKPEGTGLGLAVTAEIISAHHGQIRVSSTPGQGSAF
                     YIYLPASPE"
     gene            complement(12116..12706)
                     /locus_tag="ACZPD3_00225"
     CDS             complement(12116..12706)
                     /locus_tag="ACZPD3_00225"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR1414478.1"
                     /translation="MEELKKAPVTVLLILANILVFTAVEFTGGSEDTMHMLQCGAAYT
                     PAIMQGEYYRIFTSMFLHFGPQHLGNNMLVLFVLGGRLERTVGKLKYLLIYLLGGMGG
                     NLLCLFLELDSADFAVSAGASGAVFAVMGAMIYAVIRGRGHIEDLSARQVVIMAAFSL
                     YFGFTSEGVDNAAHVGGLICGFLLAVLLYHPSQRAG"
     gene            complement(12791..14260)
                     /locus_tag="ACZPD3_00230"
     CDS             complement(12791..14260)
                     /locus_tag="ACZPD3_00230"
                     /inference="COORDINATES: protein motif:HMM:NF039422.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HIR1414479.1"
                     /translation="MNHNYLIVYGENEINTDSYQVRMLAGNVIPSLLKCRIQGMDGRF
                     LIYFDITSKQAVNVLYEEKKMGVEDLRLIFGGFVKAMEDAAEYLINPGQFIMLPEYIY
                     TDIEKRQIYFCMMPGYEKDIKEQFQLLTEYILPKIDHQDQDAVILGYGVYKRAMEDSF
                     HLEHIKEELYKTQSSDVNGEKKEKINAKKTEQEMEFAEEETFPEDNENRNEFVREGED
                     SKEPGRLNPVGVIVPAAVLICGLAAAILKGYLPHVEMETILGVIVIAIAGIMLGLRII
                     KTKKVLHLPEQAYASGETVRHIEKIRNMPSWNKTEKKTSGKRSGWKEAEGKVSGQKWE
                     DRLYENLSQTVERNQQTNQSQPVNGQKMSDCGQKTSKSSRIHMDYGETVVLSAGTVSG
                     PASLVSKEPGELATIYLNEDLTVIGKLETACDAVVSLPTVSRIHAKIRKKEENYFLSD
                     MNSRNGTSVNGRLLRPDEEYQLEPEDEVDFAQARYIFLE"
     gene            complement(14291..14752)
                     /locus_tag="ACZPD3_00235"
     CDS             complement(14291..14752)
                     /locus_tag="ACZPD3_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HIR1414480.1"
                     /translation="MKDNFCITNEYSIIRKGSFTIETACVMPLILLVLMGLIYLSFFV
                     HNRAWLTAAAYESAVSGSMEGIKKNGEIYDTARMRSEELGSIGFFGAENLGTQTNVGK
                     EVQVTYDLDTISSYGNLSWHLRTEGKSAVINPVKHIRRLRAAQAVLREMGE"
     gene            complement(14749..15204)
                     /locus_tag="ACZPD3_00240"
     CDS             complement(14749..15204)
                     /locus_tag="ACZPD3_00240"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR1414481.1"
                     /translation="MWLNFAGEMYMEMNGVKEICVLGFLGINSWIDIRKKQVALLLII
                     IFAVCGTVWTIYSRRNVPEVLMCVGTGFLFVLISILTEGAVGMGDGWLLMALGTVLYP
                     EEFFSTLFMGMICSAVWSGIMMMGFCRKGSTEIPFVPFLLAGYLGGFLI"
     gene            complement(15195..16034)
                     /locus_tag="ACZPD3_00245"
     CDS             complement(15195..16034)
                     /locus_tag="ACZPD3_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HIR1414482.1"
                     /translation="MLLQKEKQSIEEIGKKDKKIYERRKVSLSNIYDLLFPQKSRKYF
                     RTNAKEHIGEVPSVKVSFCTSFRGKIKKGSLAVETALVLPLFFLGMVTLISFMDIYKL
                     QTEHLTALCTKAKQAGMYAYLPGGDSVDDITLPDIYTYKPFGGLIPLPDVGVYNHVKV
                     HAWTGTEFPDNEGEQGETEPMVYVTASGSVYHKNPGCSYLNVSLKQIPGSSAKSASNK
                     YGEHYSACEICSRNQNPAGVVYVTEQGNRYHNLESCSGLKRSVRLVKASSVSEMGACS
                     RCG"
     gene            complement(16059..17540)
                     /locus_tag="ACZPD3_00250"
     CDS             complement(16059..17540)
                     /locus_tag="ACZPD3_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HIR1414483.1"
                     /translation="MKYCGKERGSITLFLALILSLLLSLVCTSIESVCMASARTQILN
                     SMDIGLYSVFGQYDRKLLEEYDLFALDGSMGGGQLNLAKICDNLESYMKPVLKQNSQK
                     LELHQSGLTGYRLLTDECGEVFYQQIVQYMQETLGSQGVQLLLNKMSDRERKTEEADL
                     KAKQAETGGSIDRYDSEMNQASQKSRQAAQEAENRQQQEGQISTQPAPTQPQADNPIS
                     IIKRIMKMGILELVLPPGREISTRTVSKDTMVSGRQLQQGMEMPDGVTADSSYTSGVL
                     FQQYLMNHLGNYTDPSKESLAYQMEYVFGGRDNDIDNLKSVASKLLFIREGVNFACLM
                     ADNVKRTEAQALAAAIASGFLVPPAAVVIESALLLCWAFAESVLDVRELFAGGKIPLV
                     KTSADWQISLSNLSSLMEGLDSMRKNNEHGLSYEDYLQVLILPVSKEKKVMRAMDMIE
                     DAIRKKGRANFYMDSCVVALEAFAEVKANRKKEFQVIRQYCYE"
     assembly_gap    17656..17665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17796..18830)
                     /locus_tag="ACZPD3_00255"
     CDS             complement(17796..18830)
                     /locus_tag="ACZPD3_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1414484.1"
                     /translation="MWVHVVIFVMILCALTGWKKGWIPSSGIGGKDGIRLFAVVAIAG
                     NLLGMILTLQNGGQVYPQGYRLEKEDTGSYEKEFMVSVDGEEPVSMYIQVPEKELEEQ
                     EEEPKQNKKLTRKEQAQKSIAEAVSRYNEQRSDPDYYYLPDKLNGKKVKWESPADTSG
                     MLLTGLFLIAAMAILVMKGREEQVQLQKRYEELLMDYPGLIMKFTLLVQAGMTVRKAF
                     QKISLDYGRKRKRNPRPAYEEIRIVCYEMESGVSESEAYRRFGERCGQAKYKTFATLL
                     IQNLQKGSRQMADMLERESTEAWEERKRKARVLGEAAATKLLVPMIMMLIVVMAIVMI
                     PAFMSFYGET"
     gene            complement(18839..19612)
                     /locus_tag="ACZPD3_00260"
     CDS             complement(18839..19612)
                     /locus_tag="ACZPD3_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR1414485.1"
                     /translation="MKGKEKEQLYQKRNYDHYRFSMKELLKYMGQALALCIMADYLFY
                     KSKWVLLLMLPVPVFYLKWQKNRMIRERRKNLNYQFKDALTSLSVAVQAGYSVESAVK
                     TCVRDLERLYGKGTDIVEEFRYIESQQHISVPLEELFLDLGERSQIEDIENFASVFYT
                     AKRTGGDMNRVIQKVSRMLGDKIDVKKEIEATLAAKKSEQMIMSLMPVGIILYLQMTS
                     PGFLSVLYGNPFGIAAMSICLVIYAAAYWLGRRIVDIEV"
     gene            complement(19609..20799)
                     /locus_tag="ACZPD3_00265"
     CDS             complement(19609..20799)
                     /locus_tag="ACZPD3_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HIR1414486.1"
                     /translation="MNKDVFRKLRQMLMEELDLSRELSDKEILDVIDELILNQIKDVR
                     LALKEKVQLRQELFYSVRKLDVLQELVDDETVTEIMVNGPDTIFVERAGKLMKWHKSF
                     TSAEKLEDVIQQIVGKCNRVINESMPIVDARLENGSRVNAVIYPVALNGPILTIRRFP
                     EHPITMEKLIALGSITQECAEFLEKLVKARYSMVIGGGTGSGKTTFLAAMSEYIPRDE
                     RLITIEDNAELRIRGIDNLVRLEAKMANMEGAVSVTIRDLIKSALRMRPDRIIVGEVR
                     GGEAMDMLQALNTGHEGSLSTAHANSARDMLSRLETMVLMGVDLPLEAIRRQIASGVD
                     ILVHLGRMRDKSRKLLEVTEVCGFENGEIRIRPLYQWQEGKGLVKTASLLHVEKLERA
                     GIEL"
     gene            complement(20835..>21097)
                     /locus_tag="ACZPD3_00270"
     CDS             complement(20835..>21097)
                     /locus_tag="ACZPD3_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414487.1"
                     /translation="IQLLDMCKKIYMPVLSDAVSQCKIAQFENLVRIWDYPQILEKTE
                     KINPPFHMATCLSPAYVEQLMWSELGDYVRNLLRKESQKKEN"
BASE COUNT         5553 a         5236 c         3601 g         6697 t
ORIGIN      
        1 atgattctgt gattctctct ggcaggatga ttaactcttc ctgcatatcc ttcgttctcc
       61 gggcaaagaa gacatttcgg ataactgctg gattttacaa gcttggcagc agcgattgcc
      121 ttcggatctt tttccggttt ggaaagattg atggtgatat cgattttacc atattcactg
      181 tctactgtcc atttctgatc tttctttaca cgataacgac gaatataatc gctgtcctga
      241 ctgagtttat agaagtaatc ggttgcttcc tgtggatctt tctcatatct gctccagaac
      301 tcattctgta cctgtgccgg acgcggcatc agacagttca taagtctggt atcaaaaagg
      361 tctctgtaca caacactgtc ttctatgaga tttctcttca cagcctcatc cagaagtgca
      421 tttaatacct cttcaagatc gatatccctg tactcttcct caggttccac atattcgtct
      481 tctttaaaga catccagaag aagatttatt gtataattct tctcgcaggc cggtgtaagc
      541 cctgtttcta ctccgtactg tactaatttt ttaatgttct catataacat ctgtacgttc
      601 ctccgtttcg ctgttactgt ttcactctta cagtaacgtg tttaaacaat aaatttctct
      661 gccactgttt gtccttttta gaatactcaa atatcagcga aatgtaaaga agcattttta
      721 caattttttg gatgaataac cggcaggttg ctgtcgcgtt actgttcgct ccgttctcag
      781 ctgcctgccg gttatgtttt tcatcagtaa tttactgttt ctatataggt gataaacaca
      841 tttccggctt tctggaaaag atccgtactg tcattcattc catacggata ggtctctttt
      901 ttaaccggat catacagcca agtattatat tcatcatatc ctacgatcac tacgcttgta
      961 tctgctcccg ttttggcaat taccggacgc tgtgcactta tctcgtacag cacactgtcc
     1021 agactgcagc cggaaagatc gatcacagtt ccggtctttt tgaggctctt tttcagggtt
     1081 ttaatatcat aggttccctg aagcacactc tccggaatcc cttctgtatc tatctgcatt
     1141 tttgtctttt tatttccacg ttcccataca tactgctgtg tacggttcag cactacacct
     1201 cccagcgtat ctgcacgcaa tacagccttc gccggatctg tatagatacc gtccagtccg
     1261 ccctgtccgt aaacataata tgctgtttcc tgaggaatct gagtgtccag aacaatgtct
     1321 ttccgatcgg aactgcttac ctttgcatac atagtcagcg gagaatctgt ctgtttggtt
     1381 gtgttaaata ccagtttcac acgaacacct gtcctggatg cagaaataag ttcgattttt
     1441 actgtatttg ccgctgcctt tttattattc atgatcgtat ctttcttctg ggcaacataa
     1501 gaggtttctc cggattttgc agaaagttca aactcgatca gagtatttcc tactgaaata
     1561 tcagtgatat aaagtccgtc tttccggtat tcttttttta catttccttc aaaatcttca
     1621 atgcgcagaa tgcggatccc aacagattta tgtccctcct catctgtaag aatatcttct
     1681 ttattcagca ttccataaat cagatcttca ttcataaatc caactgtacg aagtctctgg
     1741 cctttctgcg gtgcgatcag tcgggtttta caggtatcaa aatcaatttc cttgatattt
     1801 cccttttcat ccccctctga aaccagccag gctgcatggt cattattatc ggaagataca
     1861 aaattcgcat tttttattcc tttttcaaga atttcactgg agttctcttc gatattgatc
     1921 ctgtaaagat tcttcgccag aatcagaaac agctcattct tttcattgac ataggacagt
     1981 ttttcaagat cttttttcag aaattcaaat gattccgaaa cagggataaa ggctctttct
     2041 tctgccacgt ttttatcacg attgtaatgg taaactgcaa ttccttcata tccttcatgg
     2101 cttcctctgt tcatatatcc ataaagtacg aaatcaatat ctccttcatc agttactctg
     2161 acaatcttga tgtcatgctg agttctggaa tcacggaaat ctccgtcctt caattttctg
     2221 aaactgaaaa cctgatttac cttttcattg cccggtgaat aggaccacag atctccctcc
     2281 tgcacaaaag ctatcacagt agcagcatca ttcatcatat attctacatt cttatcgcgc
     2341 actccaagga taatcccatc atcctcataa acaggcagtg taccatcaaa gacctgattt
     2401 gcagaacgct ggaaatccag cagataaatc ctggtatcct gatatttcat tctgtaaaat
     2461 tcttttacat catataattc cgtttcctca tcctcattct gcgaagtcag ctgatattcc
     2521 agcacaacgc tggcagttgt ctcattgatc tctttgatca caggaattcc ctcctgacac
     2581 agctgcggag caagattccc ccaactgatc tcactcaggg aagaattaat attaattccc
     2641 gagtaattcg ttgctgctcc tgttgtctgt ggttccagat aacttcccag tgcatctgct
     2701 gactctttgt caagacacgc ttcataaaaa tattttacaa aagctgcata atctgatgca
     2761 tgtacctgtg acctggaaac aactctcgta taataatatg ctgtaccctc atccagttcc
     2821 agggcgatct gcatggaata ttcctgattc atacgcagat cactgccaat ctccacgtct
     2881 acggacaaat actgatcttc ttttacaaga ttcttaatct tcttattttc aaggacttta
     2941 cttccatcag atgtacggat ctcataagaa aaactcttta cttccgaatc atagggattc
     3001 actttaaatg tcagttcttt tgatgtatcc agaggagtca cactgtctct ggtaaaatcc
     3061 gcctgcatag gctgcgcata tccatacatc cggtttatca gcgtatcatt catatccacc
     3121 atgacttccg gaaacactgc gtcattaaaa tcagaacggt tatctgtgga ttcactgtta
     3181 agaagcaatg cagtacctgc tgtaccaagc agaaatacca gaagaagaat acctgctttt
     3241 ttcagtatcc ttttcatcgc acactccctt cctgttcttt ttcattcaga agcctgtcca
     3301 gtgtaggatg gatattcagg gctgcaagca ttttctccac cttaccctgg tctgatcttt
     3361 tccctgttct gactgctctg atcaggagat tcttaggtgt atgttccata tcaataaact
     3421 ccaggatctg ggtatcataa cctttgctct ccagaagatc tgcacgcaca gcatctgtga
     3481 tcagcgcaga catcctttct ttcaggattc catagcgcag aaccggctcc agcatttcat
     3541 ttttgatctg tttattaact tcatgctgac agcagggtac ggaaagaatg acttcagctc
     3601 cccattccac tgccttggca agtgcataat cggtagctgt gtcacaggca tgaagcgtta
     3661 ccaccatatc cacacaggat acaccttcat aatctgcaat atcaccatga tagaaatgca
     3721 gcttctcata tccgtatctg agtgccagag aattacattt ctcaattaca tctgttttca
     3781 ggtcaagacc tatgatattt acatcatatc cttttagttc tcttaaataa taatacatgg
     3841 caaaggtaag ataactcttt ccacagccaa aatccagaat cgttatttct ctgtctctgg
     3901 aaagtctggg aagtatatct tcaataaatt caaggaaacg attgatctgt ctgaatttgt
     3961 catatctggc atggataatc tttccctgtt cattcataac tcccagatcg atcaaaaacg
     4021 gaaccggaac gccttcttta agaatatatt gtttcacccg gttatgtgcc tggatctgaa
     4081 ctttttcttt ctgttcatgg tgtttctgtt taatagtcat tttccccttt ttactgacaa
     4141 gaacaacccc gtctgttact gcaccctgtg cctgaaactg tctgaaattc tctgcaagtt
     4201 ctttttccac atattcaatc agttccgtac gcccgtagtt tttatgcaga actttctgtc
     4261 cttctgtggc agatgcctga taacagatct gtcctttcag ccggatcgga cgaatcttta
     4321 tttttgatat gccatcttta tttctgccgg cactcagaac agcctgtatc agattttcat
     4381 ttatctgttc ttctaaatat tctcttaaat ttttcattgc tctttctgtc tgttaatgtt
     4441 atataatctg ttgtaactgt ccggtgtacc agcaaaccga tttcccgaac aattacaatc
     4501 tgttttaaaa gtatctcctg cagcgatatc tgcggcatct tcttctggat atttcctgac
     4561 aaagcattac ctgtatcagt tcatccagaa aatcctccct gatagcctga gtgcttattt
     4621 ccggttccat actctccctg atcttgcagc ttaaatgata aatcatatat ttatccggat
     4681 attcatcata aaggcggctt ccctcataat ccatgagatc acattcctct tctattttct
     4741 cctgaatatg ctgtacagtc cccggataat aggatttcat caggttaaat tctttatcct
     4801 gcatccgttc tccttcatac agaagaggat ttgcatatgt ggtataaaac ggaaaatatt
     4861 ctttcagata catcgtacga tcctgcattc ttttcgattg tctatttcag tatatgcaaa
     4921 gttctgcaga ctgtgccgaa atacagttca cgtgccgctt tatttcatct gcttcttgca
     4981 gattacaatt cctggatcat tcttacagtt aatttgacac tgtgttcaat tgccgccatc
     5041 tcaaactcag cgtaatccat gtgagcactt ccgtctgcct tatcagagat tgctcttact
     5101 acaaggaatg gaatatggtt cagataagca gcctgaccga tagcagcacc ttccatctct
     5161 actgcaaatc cgccaaattc tgaaatgatg gcatctttaa cgctcttatc tgagataaac
     5221 tgatctccgg aacagattct tccttcaaat acactgatct caggatttac ttctttacat
     5281 acttttgcag ccagtttacg aagtttttca tcagcaggga aggaaaattc atccatctgc
     5341 ggaatctgac ctttcttata tccgaagccc attgcatcca tatcatgatg caaaacatct
     5401 gtggaaagaa caatatcccc aatatttaca tcattattca gacttcctgc aattcctgta
     5461 ttgatcaccg cttccacacc aaatacatct gcaaggatct gtgtgcacat acctgcgttt
     5521 acttttccga ttcctgagcg tacaacaact actttctttc cttccagagt tccctcatag
     5581 aaatccatac gggctctgga tgtaattgtg acatcctgca ttttttcctt gatttttgca
     5641 acttcctgtt ccattgctcc gataattccg atacatttca ttattaaaac acctcttctt
     5701 tgtatattgt aatttttcat tttcatggta tcaggttcag atgtgtaaac agtgacaaat
     5761 gttcctgaca tttctttatt atagcaaaac tttatttata tggaaacttt ttttatcctc
     5821 tgttataata caattgctat tttatgttac tgttcacata tcactattcc gcgaggtgtt
     5881 ttggcatgaa tatgccaaaa tcccgagaca tacaagccaa aattccattg ccgcaggcaa
     5941 tctggaataa attttggcta cgttactgag aacgaagtaa acagtaacgt agtggtatat
     6001 gaacagcagc atttatagtt acattttttc acacaggagg aaacatatca tgacgtataa
     6061 aacaaaaggt gtctgcgcca gccatattga atttgaagta gatgacaata agaaagtcca
     6121 caatgtccgt ttcatcggcg gatgcagcgg aaatacccag ggtgtagcca gtcttgtaga
     6181 gggaatggat gcagaggaag tgatcagcag attaaaagga atcaaatgcg gatttaagaa
     6241 tacctcatgt ccggatcagc tttccactgc actggaggaa gttctgaaca aataaacaac
     6301 tgtggaaata ctgaacagtt acgcaggtga ccatataaaa tatcccacga aaactatagc
     6361 aagcctctta aataatgtaa attgaatcaa tatattattt acaaagattg ctatagataa
     6421 agtttcgtgg gatatttttt taatttacat atctgtcctg tcccatttat cacacaggga
     6481 ctgaacatct cttgaaaatc tttccagatc tttattcggc gttccttcat ttttcctgat
     6541 aaccgtaata agcctgtaac caagtgccag aagtttctcg aaggctctgg ctgctcttgt
     6601 tgccttcggt gatgcagatg ctttctggat tttcacaccc tttgcctcat acagacatac
     6661 atcgtctgcc agatcataaa ccgtaccgct gaacggagcc attgtatcat accccagttc
     6721 atctgtcaga cgttttgcaa aaatctctgt aactgtatca tctccatgag taacaaagac
     6781 tttctgcggt ttctcctcaa atgccttcgc ccagtctaaa aggccattca catccgcatg
     6841 accgctgatt cccggcatct ggcagatatg cgctgccaca tgaacaggtt caccaaacag
     6901 tttcacatct gcagccccct cgatcagtgc tcttccaaga gtaccgaccg cctgatatcc
     6961 tacaaaaaga atcgtattct tctcattcca cagattatgt ttcagatggt gcttgatacg
     7021 gcctgcatca cacataccac tggctgaaat gattaccttg ggctcctcat cataattgat
     7081 cgacttcgaa tcatcactgg tgacggaaac cttcaatcca gggaaactga ttggattaat
     7141 tcccttttgg atcagttcca tcgcttcttc atcaaaacag tcatactgat gctccgagaa
     7201 aatcgtggta gcttcatttg caagaggact gtccacatat actttaaatc cgtcatgtcc
     7261 atacaccagt ccgtctgcct tgatctgtct gataaagtac agcatttcct gcgtacgtcc
     7321 cactgcaaaa gaaggaatca caagatttcc tcctctgtca aaggtttcct ggatgatttc
     7381 tgacaacagt ttcacataat caggcttttc tccatgactg cggtcaccgt aagtggattc
     7441 cataactata taatctgcat ggtgcagata ctcaggatcc ttgatcaagg gctggcaggt
     7501 atttccaata tctccggaga atacaatctt tttctctgta tcctcctccc tgatcgtcag
     7561 ctcaatactt gcagagccca gcaaatggcc tgcatcaata aaccgggctg agattccctc
     7621 tgcaggagtg atcatcttat tgtacgggca tcccacaaag tttctgatca ctcccatggc
     7681 atcttccatt gtataagccg gaacaaattc cggtttgccc tgtcgtctgc ctttacggtt
     7741 tctccattct gcttcaaaca tctgaatgtg cgcactgtct ctgagcataa tatcgcataa
     7801 atggctggtg gcatctgtgg catataccgg tcccctgaac ccctttgcat aaatagccgg
     7861 aagattacct gagtgatcta tatgtgcatg tgtcagaaga acaaattcta tctctcccaa
     7921 agccactggt atttctgcat tttcataata atccggtccc tgctccattc cgcagtctac
     7981 aagaaacttg tgacctgctg cctccagata ataacaggat cccgtaacct catgagttgc
     8041 cccgataaat gtaatcttca tataaaaacc tcccttcaga ttttgatgta ctctcggaat
     8101 ctttttgtac ttgattttat gagaagattt tttgtcagat gtgcccgaat ttttgaggcg
     8161 tacgcggtgc gtacgttgat aaaattcggg tgaatctggc gaaaaatcag ccataaaagc
     8221 aagtgcaata aagactccga gagtacattt ttgatatccc caattgtttt gctgcatttt
     8281 gcctgtttaa cccggcagct ttttactgct tttatgatcc ttctatattg tattaattat
     8341 acactacaat gaaacaaatt aacaatatct tttctgaaaa caaaaagcag aaacagtttt
     8401 tttcgactgt tcctgccttt ctttgcatct gccacgtaag aattttaaaa atttgccgat
     8461 attttaatga ttctctgtcg gtcagtgcat aaatatataa cgttttgttc cgagcctttg
     8521 atccgcacac ttatccgaag aataaaatgc cggatttctg cttcgcaaac tttcattaca
     8581 tatactatgc gaatcaaatc ggcaaatatc acacaaaaaa ataaaatttt tttatttttt
     8641 ttcaaaaacg gatgtcttct gatatgtctg cgtattttta tccggatatc gatagtcagc
     8701 tatagcagtc attcccacca gcgcagtcaa cataactact gccactgcgg cagctgcaga
     8761 tttcaggatt ctgtgagtcc tgttcccgtc atactcaaga ctttcaagtt tttcctttgc
     8821 cgccccggtg cttcctgtct tttcagccct ggctgcttca ttgaaatccc ctgtttctct
     8881 cagtcgggtg atcagccgat cataccccgc ccggatcttc tcctcggatt cttccggaaa
     8941 ttcatcccat gaggttttgc ctgctacgtt cagcaatgcg gattcaagta ttttggtttc
     9001 cacatcatac tgctcttgaa gtattttgtc tatctgctga ttttttgtct gtatatctgt
     9061 tttgttcttc ataatattta actttccttg acaatttctc cccctgggtt catattaaat
     9121 agtaacgtat agcaattcca taaaataatg aatccggaca ttttcgcagg atttcacata
     9181 ttccctgcag tcttttatag aattgttgta actattcaga ataaaacagg aggaatctct
     9241 atgaaacata ttgtgctcac cggcggcggc acagccggac atgtgactcc gaatattgct
     9301 ctgatcccac gccttaagga gcttggatac gaaatttcct atatcggatc ctacgatgga
     9361 atcgaaaaaa agcttattga agaaatgaat attccttatt acggaatctc atccggaaag
     9421 ctgagacgtt attttgatct gaaaaacttc acagatccat ttcgtgtact gaaaggattc
     9481 ggtgaggcca aaaaattatt gaaacaatta aaaccagatg tggttttctc caagggtggc
     9541 ttcgtgactg tcccggttgt aatcgctgcc ggtcgacgta aaattcctac catcattcat
     9601 gaatcagata tgactcccgg acttgcaaat aagatctgca ttccatctgc cacaaaagta
     9661 tgctgtaact tccctgagac tgtaaaaagt cttcctgctg acaaagcagt acttacaggt
     9721 actcctatcc gtcaggaatt attaaacgga agtaaagagg cagcccgcga attctgtggt
     9781 ttcacagatg acaaaccggt tcttatggtg atcggcggaa gtcttggtgc tgcatctgtc
     9841 aatgaaaata tccgcaaaat ccttccggaa cttttaaaag agtttcaggt tatccacctg
     9901 tgtggtaagg gaaaaatgga tgaaagtctc aaagacacaa aaggatatgt acagtatgaa
     9961 tatattaaac aggagcttgc cgatctgttt gctctgtctg atatcgttat ctcccgcgca
    10021 ggtgccaatg caatctgtga actgaatgct ttaaaaaaac ccaatcttct gatcccgctt
    10081 tctgcaaatg ccagccgcgg tgaccagatt ctcaatgcac gttcatttga acgtcaggga
    10141 ttcagcatgg tacttgagga agaagagatt acagaaagca cacttttaaa cgcaatccga
    10201 gaactgtacc agaacagaga gtcctatgtt catgccatgt cagaaagcag tcatatgaat
    10261 tctattgaaa aaatcaccgg gctgatcgaa gactgcgtaa ataaacagta atcaccgggt
    10321 tattttctta tattccctgg caagccagcg tctcgtcctc atgactctgt tattcacatt
    10381 attttctgtg atatgataat gtctggcaac gagacgcgtt ggtatgtcat ataacgttac
    10441 actgacatag atttcataat ttgtcctgtt cttttctctc agtttttcaa atatcgactg
    10501 caggtatcct gctgcctcca gattcagaac aatctcatct acacgattac cttttcttac
    10561 ccgcatatct gtcatttcca gaatcgcatc tgagttcgca tattcatatt tcctgaaggc
    10621 ctttttgcga tgatcggatg ccttatggta gctgattctg acaatcagat tctccagttt
    10681 cttttcatct gaaaaatcca cactccggcc catatgaaac aaagtctcaa atacatcctg
    10741 acagacatct tctgcttcgt cttcatcgcc aaggataaat tttccaatat tgaacgcccg
    10801 agagcggtat ttcctgtaga tacttacaaa atctatattc tccatctttt gcaatgtgct
    10861 gtatcagaat atcaggacag ctgctccttt acttttaaca ggatttcttc tttatctgca
    10921 tcactgagtt cacccgggtg ccatgtgatt cccacctgat ctcctttata tctcggaatc
    10981 agatgcatat gaaaatgaaa tactgtctgc cctgcacact cgccattatt ctgtacaata
    11041 ttaaatccgt cacatttcag tgctgctgtc attgctgtcg ccattttctt tgcaagaatc
    11101 attgcctttg cagccatttc atccgataat tcataaatat tggcagcatg cgatttcgga
    11161 aggatcagcg catgtccctt actcgccggg cccaggtcaa gaattaccct gaaattctca
    11221 tcctcataaa gtgttgcagc aggaatctct ccattagcga ttttacaaaa aatacaattt
    11281 tccatcttta aatccttctt tcttaaaatt caagattttg atttgcaata atcagtaata
    11341 cttgtcgttt tattgcgaat ttttcaggac gaaatcagtt gcggctgaaa gttcacaatg
    11401 atacactttt cctgaggtga taatatgcag attgaaataa caaaacaatc agaaaaagat
    11461 tttcgttttc ttttatccag actttcacat gaaatccgta atccggtcac gctgatcaac
    11521 agtgaacttc agttgatggc ttcctctcac ccggaactgt gcagttacag acaatgggac
    11581 agtcttatgg ataaccttga atatgtaaaa gaactgctga aagaactctc tgacttcagc
    11641 catgcgcaga cagttactct tgttcccgta aatcccgcgg aatttctgac cactgtttta
    11701 tcctgtcaga aaaacactct ggactatctg ggaatccggc tggaaataca tgtggaacat
    11761 acttcttcac ctgtttttct tgaccggata aaaatgcgtc aggtcatatt taacctgatc
    11821 aaaaattcct gtgaagccat cgatcagcca ggcggaaaga ttattgtaaa tctcacccct
    11881 gcagattccg gcatctgcat ctgtattaaa gataacggat gtggtatgac gcataagcag
    11941 actgagaata tctttcatcc cttcattaca tataaaccgg aaggaaccgg tctcgggctt
    12001 gcagtaacag cagaaatcat ctctgcacac catggtcaga tcagggtttc cagcactccc
    12061 ggtcagggtt ctgcctttta tatttatctg ccggcatccc ctgaataaag gtcggttatc
    12121 ctgcacgctg cgaaggatga taaagcagaa ctgcaagcag gaatccacag ataagaccgc
    12181 ctacatgtgc cgcattgtcc accccttcgc ttgtaaaacc aaaataaagc gaaaaagcag
    12241 ccatgatcac tacctgtctg gcagaaagat cttcaatatg acctctgccc ctgatcacag
    12301 catagatcat tgcacccatc acagcaaaca ctgcaccgga tgcacctgct gaaactgcaa
    12361 aatccgcact gtccagttcc aggaacagac acagcagatt tccacccatt cctcccagaa
    12421 gatagatcag cagatatttc agttttccaa ctgtacgctc cagtctgcct ccaagcacaa
    12481 acagtaccag catgttattt ccaagatgct gcggtccaaa atgaaggaac atacttgtaa
    12541 agatcctata gtattctccc tgcataatgg cgggagtata tgccgctccg cactgaagca
    12601 tatgcatggt atcctctgat cctccggtaa attccactgc agtaaaaaca agaatattcg
    12661 ccagaatgag aagcactgta accggtgcct ttttcagttc ttccaagttt tacctctttt
    12721 ctgtttcttt atctcaaaaa caatgtacca ttactaattg tagcagacct cctgtatttt
    12781 gtaaaaacat ttattccaga aatatatatc ttgcctgtgc aaaatccact tcatcttccg
    12841 gttcaagctg atattcctca tccggacgaa gtaatcttcc atttacagac gttccgtttc
    12901 tggaattcat atcactaagg aaatagtttt cttctttttt tcgtattttt gcatggatac
    12961 ggcttacggt aggcaggctg accaccgcat cacaggcagt ttccagcttt ccgatcacag
    13021 tcagatcttc attgagataa atggtcgcca gttctcccgg ttctttgctc accagacttg
    13081 caggtccgct tactgttccg gcagatagta ctactgtttc accatagtcc atatgtatcc
    13141 gggatgattt ggaagttttt tgtccacagt ctgacatttt ctgcccattc accggctgac
    13201 tctgatttgt ttgctgattt ctctccactg tctgggacag gttttcatat aatctgtcct
    13261 cccatttctg ccctgacact tttccttctg cttctttcca tcctgatctt ttccctgatg
    13321 ttttcttttc tgttttattc catgatggca tatttcttat tttttctatg tgtctcactg
    13381 tttctcctga tgcatacgcc tgttccggga gatgcaaaac tttttttgtc tttataatac
    13441 gcaggcccag catgataccc gctatagcta taacaattac accaagtatg gtttccattt
    13501 ccacatgtgg cagatatccc ttcaggatag cagctgccag tccacagatc agaacagcag
    13561 ccggcacaat aacaccgacc gggttcagtc ggccgggttc ttttgaatct tctccttctc
    13621 ttacaaattc attccggttt tcattatctt ccgggaaagt ctcttcttct gcaaactcca
    13681 tctcctgctc tgtctttttt gcatttattt tttctttctt ctccccattt acatctgaac
    13741 tctgagtttt atataattct tctttaatat gctccagatg aaaactgtct tccattgctc
    13801 ttttatatac tccatatcca aggattaccg catcctgatc ctgatggtct attttcggca
    13861 gaatatattc tgtcagaagc tgaaactgtt ctttaatatc cttctcatat ccgggcatca
    13921 tacagaaata aatctgtctt ttttcaatat ctgtataaat atattccggc aacattataa
    13981 actgtccggg attgatcaga tattccgccg catcttccat agcctttaca aaaccaccga
    14041 aaataagcct gagatcttca actcccatct tcttttcttc atacagcaca ttcaccgcct
    14101 gtttcgaagt aatatcaaaa tagataagaa atctgccatc catcccctgt atcctgcact
    14161 tcagaagaga gggaatcaca tttcccgcaa gcatccttac ctgataggaa tctgtattga
    14221 tctcattctc accatagaca atgagataat tatgattcat atccctctta tattctgtcc
    14281 gcatactatt ctattccccc atttctctca gtaccgcctg cgccgcccgc agtctgcgga
    14341 tatgtttcac cggatttatc actgcggatt tcccttctgt cctcaaatgc cagctcagat
    14401 tgccatacga cgagatcgtg tccaggtcat aggttacctg cacttctttt cccacatttg
    14461 tctgggtacc taaattctcc gccccgaaaa agccaatact ccccagctcc tcactgcgca
    14521 tccttgcagt atcatatatt tctccgtttt tcttaatccc ttccatgctc ccgcttactg
    14581 ccgattcata agctgccgct gtcagccatg ccctgttatg tacaaaaaag ctcagataga
    14641 taagtcccat caacaccagc aggatcaacg gcatgacaca ggcagtctcg attgtaaaac
    14701 ttcccttacg aattatactg tattcatttg ttatacaaaa attatctttc atatcaaaaa
    14761 tcctcccaga tatcctgcca gcagaaatgg cacaaacggt atttccgtac ttccctttct
    14821 gcaaaatccc atcatcatga tgccggacca tactgcactg cagatcattc ccataaaaag
    14881 tgttgaaaaa aattcttccg ggtacagcac tgtccccagt gccataagca gccagccatc
    14941 gcccattccc acagcacctt ctgtaaggat gctgatcagc acaaagagaa aacctgtccc
    15001 cacgcacata agaacctccg gaacatttct tctgctatat attgtccaca cagttccgca
    15061 gactgcaaat attatgatca gcagaagagc aacctgcttt tttctgatat ctatccatga
    15121 atttattccc agaaatccaa gtacacatat ttcttttaca ccattcatct ccatatacat
    15181 ctctccggca aaattcagcc acacctgcta catgctccca tttccgaaac agaggaagct
    15241 tttaccagtc ttaccgaacg tttcagccca ctgcaggact ccagattatg gtatctgttt
    15301 ccctgttccg taacatatac cactcctgcc ggattctgat tcctgctgca gatttcacag
    15361 gctgaataat gctccccgta cttattggat gcagattttg cggaacttcc gggaatttgt
    15421 tttaatgaaa cattcagata gctgcagccc ggatttttat ggtacacact gcccgaggca
    15481 gttacataaa ccatcggttc tgtttctccc tgctctcctt cattgtcagg aaattcggtt
    15541 cctgtccatg catggacttt tacatggtta taaactccaa catccggaag aggtatcagt
    15601 ccaccaaagg gtttatacgt gtagatatcc ggcagcgtaa tatcatcaac actgtcccca
    15661 cccggaagat acgcatacat tcccgcctgt tttgccttcg tacacaatgc agtcagatgt
    15721 tccgtctgca atttatatat atccataaac gaaatcagcg taaccattcc caggaagaac
    15781 agcggcagta caagtgctgt ttctactgcc agacttccct tcttaatctt tccccgaaaa
    15841 gaggtgcaga aagatacttt tacagaagga acttctccaa tatgttcttt tgcatttgtc
    15901 cgaaaatatt tccgactctt ttgggggaat aaaagatcgt aaatattgct tagagagact
    15961 tttctccttt cataaatttt tttatctttt tttccgattt cttcaatgct ttgtttttct
    16021 ttctgtaaaa gcatcttttc acacctcttt tcagcaattc attcataaca atactgacgt
    16081 atcacctgaa attccttttt cctgtttgct tttacctccg caaatgcctc cagagctaca
    16141 acacaggaat ccatataaaa atttgccctg cctttctttc ttatcgcatc ttcgatcata
    16201 tccatagctc tcatcacttt cttttccttt gaaacaggca atatcagaac ctgaaggtaa
    16261 tcctcatagg acaatccatg ttcattgttt ttccgcatgg aatccagtcc ttccatcaga
    16321 cttgaaagat tagacagcga aatctgccag tctgcggaag tttttacaag gggaatcttc
    16381 cctcctgcaa aaagttctct cacatccagt acgctctccg caaatgccca gcataaaagc
    16441 aatgcagact ctatcactac cgctgccgga ggaaccagaa atcccgacgc aatggctgca
    16501 gcgagtgcct gtgcctctgt tctttttaca ttatctgcca tcaaacatgc aaagtttacg
    16561 ccttctctga tgaacaagag ctttgatgcc acagatttaa ggttatctat atcattgtct
    16621 cttcctccaa aaacatattc catctgatat gcaagacttt cttttgacgg atctgtgtaa
    16681 tttcccagat gattcatcag atactgctga aacaaaaccc cggaagtata actgctatct
    16741 gctgtcactc cgtccggcat ctccattccc tgttgcagct gtcttcccga aaccattgtg
    16801 tctttgctga ctgtccgggt ggaaatttcc cgtccgggtg gcaaaaccag ctccagtatt
    16861 cccattttca taattctttt tataatactg atgggattgt cagcctgtgg ctgcgtcggt
    16921 gctggctggg ttgatatttg tccttcctgt tgctgcctgt tttcagcttc ctgtgctgcc
    16981 tgccggcttt tctgagaagc ctgattcatt tctgaatcat atctgtcgat ggagcctcct
    17041 gtctccgcct gtttcgcttt cagatccgcc tcttctgttt ttctctctcg atcagacatt
    17101 ttgttgagaa ggagctgtac cccctgactt ccaagagttt cctgcatata ctgcacaatc
    17161 tgctgataaa aaacctctcc gcactcatct gtcagaagcc ggtaaccggt aagtccgctc
    17221 tgatgcagtt caagtttctg actgttctgc tttaacaccg gtttcatata ggattccaga
    17281 ttatcgcaga tctttgcgag attaagctgc ccgcctccca tggaaccatc cagcgcaaac
    17341 agatcatatt cctcaagaag tttcctgtca tactgtccaa atacagaata aagtccgata
    17401 tccatactgt tgagtatttg ggttctggcc gatgccatac atacagattc tatacttgta
    17461 catacaagag acaaaagcag actgagtatt aatgcaagaa ataatgtaat gcttcctctt
    17521 tcctttccgc aatatttcat acaggaagta cacctccttc tcttttcatc ttaaatagaa
    17581 ttactctgtt tggcgatctt tgaaagaatg ctctgcacca gttcggtcag ctgctctttg
    17641 aaaataatga caagcnnnnn nnnnnggatg attcctgcgg cgaatcttcg tatcagtccg
    17701 tttttctttt ttatctgtct tatcaattgt cccatttttt ccctccatat ccaaaagcac
    17761 cctttacgct gcacagaatt tactgcagca ccttatcatg tttctccgta gaaagacata
    17821 aaagcaggaa tcatgacaat tgccataacg acaataagca tcatgatcat cggcaccaaa
    17881 agttttgtgg ctgcagcctc tccaagaaca cgtgcttttc tctttcgttc ttcccatgct
    17941 tcggttgatt ctctttcaag catatcagcc atctgtctgc tgcccttctg gagattctgg
    18001 ataagaagcg tggcaaatgt tttatacttt gcctgaccac atctttctcc aaatcttctg
    18061 tatgcttcac tttcagaaac tccgctttcc atctcatagc agacaatccg tatctcttca
    18121 tacgcaggtc ttggattccg ctttctcttt cttccataat ccagagaaat tttctggaat
    18181 gcctttctta cagtcatccc ggcctgaacc agaagtgtaa atttcatgat cagtcccgga
    18241 taatccataa gaagttcttc ataccgtttc tgaagctgaa cctgttcttc tctccctttc
    18301 atcacaagaa ttgccattgc cgcaataaga aacagtcctg tcagaagcat tccggaagtg
    18361 tctgccggac tttcccattt tacctttttt ccattcagct tatccggaag ataataataa
    18421 tccggatcac tgcgctgctc gttataccgg gatactgcct ctgcaatact tttttgtgcc
    18481 tgttcttttc tggtcagctt cttattctgt ttcggttctt cctcctgctc ttccagttcc
    18541 ttctccggta cctgaatgta catggagacc ggttcttctc cgtcaacgga taccataaat
    18601 tccttctcat atgatcctgt atcctccttt tccagacggt atccctgagg atacacctgt
    18661 ccaccgtttt gcaaagtaag gatcatgccc agcagatttc cggctatcgc tactaccgca
    18721 aaaagtctga taccatcctt ccctccgatt ccggaagatg gaatccatcc ttttttccag
    18781 ccggtcagtg cacacaggat cataacaaaa atgacaacat gtacccacat ttgtactgtc
    18841 acacctcaat atctacaatc cttctgccaa gccagtatgc cgcagcataa atgacaagac
    18901 agatactcat tgctgcaatt ccaaagggat ttccataaag aacacttaag aagcccggtg
    18961 aagtcatctg caagtacagg ataattccca caggcataag actcatgatc atctgttctg
    19021 acttctttgc tgccagtgta gcctctatct cttttttaac gtcaatctta tctccaagca
    19081 tacgagacac cttctgaatt acccggttca tatcaccacc tgtacgcttg gctgtataaa
    19141 atacagaggc aaaattttcg atatcttcta tctgacttct ttctccaaga tccagaaaca
    19201 gctcttccag cggaacactg atatgctgct ggctttcaat atagcgaaat tcctccacaa
    19261 tatccgttcc ctttccgtag agtctctcca aatctctgac acaggttttt accgcgcttt
    19321 ccaccgaata tccggcctga accgcaacac tcagcgaagt aagtgcatcc ttaaactgat
    19381 aattcagatt cttccgtctt tccctgatca tacgattttt ctgccatttc agataaaaca
    19441 caggaacagg aagcataaga agcaacaccc atttactttt gtaaaaaaga taatctgcca
    19501 ttatgcacaa cgccagtgcc tgtcccatat atttcagaag ttctttcata gaaaaacgat
    19561 aatgatcata attacgtttc tgataaagct gttctttttc tttccctttc atagttcaat
    19621 ccccgctctt tccagttttt ccacatgaag gagggatgct gtctttacaa gacctttccc
    19681 ttcctgccat tgatacagcg gccggatacg gatttctcca ttttcaaagc cgcacacctc
    19741 agttacttcc agcaatttcc ttgatttatc cctcattctt cccagatgta ccagaatatc
    19801 cactccggaa gcaatctgtc ttctgattgc ttccaatgga agatcaacgc ccatcagaac
    19861 cattgtttcc agtctggata acatatccct ggcagaattg gcatgggcag tagacagcga
    19921 cccttcatgc cctgtattca atgcctgaag catatccatt gcctctccgc cgcggacttc
    19981 tcccactatg attctgtccg gacgcattcg taatgcagat ttgatcagat ctctgatcgt
    20041 aacggaaact gctccttcca tgtttgccat ctttgcttcc agccggacca gattgtcaat
    20101 tcccctgatg cgaagttctg cattatcttc tattgtgatc agtctttcgt ccctggggat
    20161 atattccgac attgctgcaa gaaatgtagt ctttccgctt ccggttccgc ctcctatgac
    20221 catggaatat ctggccttca caagcttctc cagaaattct gcacattcct gtgtgatact
    20281 tcccagtgcg atcagctttt ccattgtaat ggggtgttca ggaaaacgcc ttatagtgag
    20341 aatcggacca tttaatgcaa ccggataaat tacagcattt accctggaac cattttccag
    20401 tcttgcatcc acgatcggca tggattcatt gatcacacga ttacattttc caacaatctg
    20461 ctggatcaca tcctccagtt tctcagcaga agtaaaactt ttatgccatt tcataagctt
    20521 gccagcccgt tctacaaaaa tggtatccgg cccgtttacc atgatctcag tcactgtctc
    20581 atcatccacc agttcctgaa gcacatccag tttgcggaca gaataaaaca gttcctgacg
    20641 aagctgtacc ttttctttca gcgcaagacg gacatctttt atctggttca gaatcagctc
    20701 atcaattaca tcaaggattt ctttatccga taattctctg gacagatcca gttcttccat
    20761 cagcatctgc cgcagctttc taaatacatc tttgttcatc ctttttccct ttacagcatc
    20821 tttccggaac ttttttaatt ttcttttttc tggctttctt tcctcagaag gttccgcaca
    20881 taatctccca gttcactcca catcagctgt tcaacatacg caggtgatag acaggttgcc
    20941 atatgaaacg gtggatttat cttttccgtc ttttccagaa tctgaggata atcccagatt
    21001 cggaccagat tctcaaactg cgcaatctta cactgtgaaa cagcatcaga caatacaggc
    21061 atataaatct tcttacacat atccagcagc tggatat
//
LOCUS       DBMDXG010000003        19141 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_882_length_19141_cov_1.662342, whole genome shotgun sequence.
ACCESSION   DBMDXG010000003 DBMDXG010000000
VERSION     DBMDXG010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 19141)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19141)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19141
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_882_length_19141_cov_1.662342"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            195..2576
                     /locus_tag="ACZPD3_00275"
     CDS             195..2576
                     /locus_tag="ACZPD3_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR1414488.1"
                     /translation="MNQKAYKALEYYKIINMLTDKASSSMGKEICRKLEPSTDIDEIR
                     HMQTQTRDALTRLFQKGNISFGSVKDVRGSLKRLEIGSSLGIGELLAICSLLENTNRV
                     KAYSRSERGDSLPDSLDGMFEALEPLTPLTTEIRRCILSEDEISDDASSNLRQIRRNM
                     KITGDRIHTQLSSLVNGSARNYLQDSVITMRNGRYCIPVKAEYKGQVPGMVHDQSSTG
                     STLFIEPMAIVKLNNDIRELELEEQKEIEVILSTLSQQTAEQTDSIRADLNIMVQLDV
                     IFARASLAMDMNATEPIFNDEGRIRLKQARHPLIDKKKAVPIDIRLGDDFDLLVITGP
                     NTGGKTVSLKTVGLLTLMGQSGLHIPTLDRSELALFEEVYADIGDEQSIEQSLSTFSS
                     HMTNVVSFLKKANRHSLVLFDELGAGTDPTEGAALAIAILSHLHEQGIRTMATTHYSE
                     LKVYALSTSGVENACCEFDVETLRPTYRLLIGVPGKSNAFAISSKLGLPDYIIDKAKE
                     QISEQDESFEDVLSSLESSRITIENERREIEQYKQEIASLKSEMESKQEKLNEQRDRI
                     IRQANEEAHAVLREAKEYADQTMKMFHKFQKDHVDLSAVEKERQNLRKHMNKAEKGMT
                     QKTAAKKPKKELTAKDISLGDAVKVLSMNLKGTVSSRPDNKGFLFVQMGIIRSKVHIS
                     DLELIDEAEITTPTMQRTGAGKIRMSKAAHVSTEINLLGKTVDEAVAELDKYLDDAYI
                     AHLKSVRIVHGKGTGALRKGVHNYLKRQKHVESFRLGEFGEGDAGVTIVEFKK"
     gene            2588..3289
                     /locus_tag="ACZPD3_00280"
     CDS             2588..3289
                     /locus_tag="ACZPD3_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1414489.1"
                     /translation="MVSKQKILIVDDDNNIAELIALYLTKECFETKIVNDGEEALREF
                     ASFRPDLIILDLMLPGIDGYQVCREIRHTSDVPIIMLSAKGETFDKVLGLELGADDYM
                     IKPFDSKELVARVRAVLRRFQVKQPSPSSSEKCVTYPDLTVNLTNYAVTYMGRQVDMP
                     PKELELLYFLAASPNQVFTREQLLDHIWGYEYIGDTRTVDVHIKRLREKIKDNPHWSI
                     ATVWGIGYKFEVKNP"
     gene            3286..4698
                     /locus_tag="ACZPD3_00285"
     CDS             3286..4698
                     /locus_tag="ACZPD3_00285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414490.1"
                     /translation="MKQHHLSTKFLRFYILIGILGFFLITLGGSYMVEKHLEHSLSAA
                     LYTEAHNIASNEAVKSNISSSTVDTLQEHLCAISDFQDAVLWIINSNGEIIVSTQKNI
                     YVREPIPLEEFDASKWGSNYYQIGKFYGFFKTDHLSVIAPITFDMETKGYVAIHYSMT
                     NLYQSRSSILFIMQVIFLLCYAATSLLLWAYSHYIRKPLARIMKGASEYAGGNLAYKI
                     DVTSDDEMGYLAKTLNYMSDELNKNGEYQRKFIANVSHDFRSPLTSIKGYVNAILDGT
                     IPYEMQERYLKIIAFESERLEKLTRSLLTLNELDMKKRMMHIQRFDINDTIKNTAATF
                     EGICTSRQIRLELLLSGHELYVRADMEQIQQVLYNLLDNAIKFSNDNSSVQIETTVKS
                     GKVFVSVKDYGTGIPKESLGKIWDRFYKIDASRGKDRKGTGLGLSIVKEIINAHNQNI
                     DVISTEGVGTEFIFTLEKTK"
     gene            complement(4884..6608)
                     /gene="ade"
                     /locus_tag="ACZPD3_00290"
     CDS             complement(4884..6608)
                     /gene="ade"
                     /locus_tag="ACZPD3_00290"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR1414491.1"
                     /translation="MELEMYRRYSQMARGIEKAELVFKNGRVFSSGTGEFIDGDVAVA
                     DGIVIGVGTYEGETEIDLEGKVICPGFIDSHLHLESTLVTPGELVRQAAQCGTTTFIV
                     DPHESANVSGTDGIDYILDQTEDAPANVYVMMPSCVPATHVDDNGCILTAGKMKGYLE
                     HPRILGLGEVMDAPSVINGSVAMHEKLQLFQDRVKDGHAPFLAPGDLAAYVLGGIDTD
                     HECVDYEYAMAEARNGMQVLIREGSAARNLDAIVKGIVEHHTDTSGFCFCTDDKHIEE
                     IRKEGHINYNVKRAVQLGLPVEKALQMATIQPARCYGLYRLGMIAPGRQADFVILDNV
                     ADLNVVDVYHCGKKIIKDEKAELKPCPPYLKNTVHVSGFSEERLKLKHPGTKARVIQM
                     LEKQIVTRDVLEEVPWIESDGEKYFAPDGEYQKIAVIERHKNTGKMGVGIVKGYGIRG
                     GAIASSVSHDSHNIIVVGDNDRDMAIAVKEMMRMQGGYTLVCNGEIYGTLPLPVMGLM
                     SDAGYESVNEALAKMIPKAHEMGVKEGFDPFITLSFMALPVIPEIRITPRGIYLVNED
                     RMLRTPFS"
     gene            complement(6888..8864)
                     /locus_tag="ACZPD3_00295"
     CDS             complement(6888..8864)
                     /locus_tag="ACZPD3_00295"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR1414492.1"
                     /translation="MSKNKGLGLVSALAVGAGVAAVAAGKKYKTAETKKKEDYDALHN
                     TSEYRNTERGKYEKNSKGIYYTNGNYEAFARPRKPEGVDDKHAYIVGSGLASLAAACF
                     LVRDGQMPGKNIHILEAMDIAGGACDGIFDPSRGYVMRGGREMENHFECLWDLFRSIP
                     SIETPGVSVLDEYYWLNKEDPNYSLCRATKERGKDAHTDGKFNLSQKGCMEIMKLFMT
                     KDEDLYDKTIEDVFDDEVFDSTFWLYWRTMFAFENWHSALEMKLYFQRFIHHISGLPD
                     FSALKFTKYNQYESLILPMQKYLEEAGVEFQFNTEVTNVLFEFEGNKKIAKAIECKVN
                     GVEKGIVLTENDFVFVTNGSCTEGTIYGDQNHAPNGDAEVRTSGCWSLWKNIAKQDPS
                     FGHPEKFCSDIAKTNWESATVTTLDDKIIPYITDICKRDPRTGKVVTGGIVSCQDSSW
                     LLSWTINRQGQFKDQDKDKVCVWVYGLFTDVPGDYIKKPMKECTGKEITEEWLYHLGV
                     PVDQIEDMAENSAVCVPTMMPYITAFFMPRTKGDRPDVIPDGCVNFAFLGQFADTPRD
                     TVFTTEYSVRTAMEAVYGLLGVDRGVPEVWGSVYDIRELLDSSVKLMDGKSPLEIELP
                     GPLDMLKKPLLKAVKGTVVEKVLRDHDVIKDYMM"
     gene            complement(9152..9733)
                     /locus_tag="ACZPD3_00300"
     CDS             complement(9152..9733)
                     /locus_tag="ACZPD3_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1414493.1"
                     /translation="MPNTTKAALEESLKRLLLKKPLDKITITDITTDCGISRMAFYYH
                     FKDIYDLVEWSCVEDGTKALQGKKTSESWTEGLTQIFGAVLENKPFIMNVYRNVDRER
                     IENYLFKLTYDLIVGVVEEKSKGLNITEEDKKFIADFYKYGFVGIMLEWIREGMKENI
                     EDLVRMMDLTLHDTVTTSIHNFQKNIMEKNTEI"
     gene            complement(9761..10261)
                     /locus_tag="ACZPD3_00305"
     CDS             complement(9761..10261)
                     /locus_tag="ACZPD3_00305"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR1414494.1"
                     /translation="MAKLNIVLHEPEIPANTGNIGRTCVATGTRLHLIEPLGFSLSEK
                     ALKRAGMDYWKDLDVTTYLDFEDFLEKNPGAKIYYATTKAPQTYTDVHYEEDCYIMFG
                     KESAGIPEDILVNNQETCVRIPMIGDIRSLNLSNSVAIVLYEALRQNNFDHMNLEGHL
                     RNYDWK"
     gene            complement(10332..10928)
                     /locus_tag="ACZPD3_00310"
     CDS             complement(10332..10928)
                     /locus_tag="ACZPD3_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR1414495.1"
                     /translation="MRLGQAAMEALRAEITGCLKPGDELVVACPVALKGTSVIAKNKK
                     DKLAERFSAGFIQNCVSLWDAYGAGSIVWKIAQEADASALYAMGEGGFLSALWKMAEA
                     SEVGLEADFRKVPIRQETIEVCEIFDLNPYKLQADGAVLIGIRGGEALVQRLRNEGFM
                     AEVIGQTNSGNDRLLYSGGSARYLERPAEDELQQLLKE"
     gene            complement(11068..12744)
                     /locus_tag="ACZPD3_00315"
     CDS             complement(11068..12744)
                     /locus_tag="ACZPD3_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR1414496.1"
                     /translation="MKILLVACNAKYIHSNLAVYDLQAYASDYADHIVLKEYTINQQK
                     DDIMRDIYLEHPDVVCVSCYIWNLSFVKELMADLIKILPGADFWAGGPEVSYDAEKFL
                     TENSEFKGVMVGEGEETFKELAGYYVEKNPQDLKDMTGICYRDGDQIIHNGWRQIMDL
                     SSIPFIYKDLSEFKNRIIYYESSRGCPFSCSYCLSSIDKKLRFRDTETVKKELQFFID
                     NKVPQVKFVDRTFNCKHDHAMAIWKYINEHDNGVTNFHFEISADLLREEELQEMSTMR
                     PGLIQLEIGVQSTNPDTIKAIHRTMDFEKLKGIVDRIHSFGNIHQHLDLIAGLPYEDY
                     DSFRHSFNDVYALKPQQLQLGFLKVLKGSHMMEMCREYGIVYKTQEPYEVLSTKWLDY
                     DHVLKLKTVENMVEVYYNSGQFQNTLEYLENFFQDAFSIYERLGSFYMEKGYGDVSHT
                     RMRRYEILLEFLEDVPEISMDQVKDQMVYDLYLRENLKSRPGFARDQKPFERQVWDFR
                     KREKVAKNAHVEVFADGTVLLFNYADRDPLTNNAHVTDVTKDVFENLNRD"
     gene            complement(12751..13053)
                     /gene="trxA"
                     /locus_tag="ACZPD3_00320"
     CDS             complement(12751..13053)
                     /gene="trxA"
                     /locus_tag="ACZPD3_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR1414497.1"
                     /translation="MAKEITTANFETEVLKSEKPVLIDFWATWCGPCMRQGPVVEELA
                     EEGYAVGKVDVDQNMALAQQFRVVSIPTLILFKDGAEVKRFVGLTSKEELKSALEG"
     gene            complement(13164..14072)
                     /locus_tag="ACZPD3_00325"
     CDS             complement(13164..14072)
                     /locus_tag="ACZPD3_00325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1414498.1"
                     /translation="MEKVIIIGAGPAGISAALYAVRGNLDPLVINNGIGALEKAEKIE
                     NYYGLEHPLSGQELYDTGIAQAKALGVRILDAQVLGVGGFDTFVVKTTEGEFETQSLI
                     LATGSKRAAPKIPGVKEFEGKGVSYCAICDAFFYRGKDVAVLGNGDFAMHEAKELSNT
                     ASTVTIYTNGEEPEFTPEENISVNTMKIQSVEGDDLVSGIRLEPDVQAEEIKAGAGQQ
                     ANDFCPAEGVFIAMGTAGSTEIARQMGAELTEKGNIKVNENMETTIPGLYAAGDCTGG
                     LLQVAKAVYEGAQAGINAGKYVRSLK"
     gene            complement(14333..15571)
                     /gene="glyA"
                     /locus_tag="ACZPD3_00330"
     CDS             complement(14333..15571)
                     /gene="glyA"
                     /locus_tag="ACZPD3_00330"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR1414499.1"
                     /translation="MYSIDDVAKTDKDIADLIEAELARQNSHIELIASENWVSKAVMA
                     AMGSPLTNKYAEGYPGKRFYGGCSCVDEVEALAIERAKELFGCEYANVQPHSGAQANM
                     AVFFAMLQPGDTVMGMNLDHGGHLTHGSPANMSGTYFKPVYYGVNDDGVIDYEEVRRI
                     AIENKPKLIVAGASAYARVIDFKKFREIADEVGAYLMVDMAHIAGLVAGGQHPSPIPY
                     ADVVTTTTHKTLRGPRGGLILSSAENAKKFNFNKAVFPGIQGGPLMHVIAAKAVCFKE
                     ALQPEFKDYAKMIVENAQALCKGLQKRGIDIVSGGTDNHLMLVDLRSLGVTGKQMENL
                     LDEVNITCNKNAIPNDPQSPFVTSGVRLGTAAVTSRGMKPEDMDKIAEAIAMTLKEEG
                     SQEKAKAIVKELTDKYPLVG"
     gene            complement(15751..16821)
                     /locus_tag="ACZPD3_00335"
     CDS             complement(15751..16821)
                     /locus_tag="ACZPD3_00335"
                     /inference="COORDINATES: protein motif:HMM:NF025598.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR1414500.1"
                     /translation="MKNKIKKILLLGMTAMFTAGAAGTAVISCPVWADEAEQDSETAE
                     EPKAEDAAVEEEIADQTDDKTENTDLKTVEHPRMSVYSIRRFSIVKGGEEVFQIKQEP
                     ADYKMDFDYWEINNPYDEIATVNTENMYEMFGVLAAFDLSNGVDAANTDTGLDNTKTY
                     FTVDFVNTVNDDTAKETQDADATATILIGNTDENGDYYACVKGYEEAVYLLSKESVNS
                     LLELKPFNLILKIPALVNIDTLDSVDMSIGKKTYTMKLDGSDYKFGKKTVKKEKFTEL
                     YQALQSIMLDSEVEETKDAADKEEVLTVTFHRNTEEAPEVTLKYFAYDDTYDSLEING
                     TERFLVKAEDVDELVKQIKKAF"
     gene            complement(16907..19027)
                     /locus_tag="ACZPD3_00340"
     CDS             complement(16907..19027)
                     /locus_tag="ACZPD3_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1414501.1"
                     /translation="MKDHRKERAEARKKAEKLVSQMTLLEKASQLKYDAAPVKRLGVP
                     AYNYWNEALHGVARAGVATMFPQAIAMAAVFDDEEMKKVGDIIATEGRAKYNAYSAKE
                     DRDIYKGLTFWSPNVNIFRDPRWGRGHETYGEDPYLTSRLGVKFVEGLQGDGSVMKAA
                     ACAKHFAVHSGPESLRHEFDAEVSMKDMWETYLPAFEALVTEADVEAVMGAYNRTNGE
                     PCCAHKYLMEDVLRGKWKFEGHYTSDCWAIRDFHEHHMVTSTPRQSAAMALNAGCDLN
                     CGNTYLHMMGAYQDGLVTEEKITESAVRLLTTRYLLGLFDGSEYDKIPYSVVECKEHI
                     DEALKMARKSCVLLKNDGVLPIDKSKVNTIGVIGPNADSRAALIGNYHGTSSEYVTVL
                     EGIREEAGDDVRILYSQGCDLYKDKVENLAWDQDRISEAVITAENSDVVILCVGLDET
                     LEGEEGDTGNSDASGDKVDLHLPKVQEELIEKVTAVGKPTIVVLMAGSAIDLNYAQDN
                     CNGILLAWYPGARGGRAIADLLFGKESPSGKLPVTFYKDLEGMPEFTDYSMKNRTYRY
                     MEKEALYPFGYGLTYSDTCVTEAEVVGEVSAESDIMLKATVKNNGTVDTDEVVQVYIK
                     DLDSPLAVRNYSLCGFKRVSLKAGEEKSVEFTISNKAMNIVDEDGNRYIAGKHFRLFA
                     GVSQPDTRSAELTGHKPAEVEIAL"
BASE COUNT         5070 a         4701 c         3407 g         5963 t
ORIGIN      
        1 catacaagcc aaaattccat tgccgcaggc aatctggaat gaattttggc tacgttactg
       61 tgaacggagt gaacagtaac acaattattt ttttagaatt gctataatcc cggagtacat
      121 ttccataaca aaaaatgcta taatatcagg gacagcaaat tataaagata tctatcagga
      181 gatttaaaca gacaatgaat cagaaagcat ataaagcact tgaatattat aaaatcatca
      241 atatgctgac agacaaggct tcctcttcca tgggaaaaga aatctgccgg aagctggagc
      301 catccacaga tattgatgaa atccgacata tgcagactca gaccagagat gcacttaccc
      361 gtcttttcca gaaaggaaat atttcctttg gcagcgtcaa agatgtgcgt ggttctctga
      421 aacgtctgga aatcggaagt tctctgggga ttggtgaact tcttgccatc tgcagccttc
      481 ttgagaacac caatcgtgtc aaagcttatt ccagaagtga gcgcggtgat tctctcccgg
      541 actctctgga cgggatgttc gaggcactgg aaccactgac tcctcttaca acagagatcc
      601 gcagatgcat cctctcagaa gatgagatca gtgatgatgc aagcagcaac ctccgccaga
      661 ttcgaagaaa catgaagatt accggagaca ggatccatac acagctctcc tctctcgtaa
      721 acggaagtgc ccgcaattat ctgcaggatt ccgttattac catgagaaac ggacgctact
      781 gcattccggt caaggcagaa tacaaaggac aggttccggg tatggtacac gatcagtcct
      841 ccacaggttc tactttattt atcgaaccta tggcaatcgt aaaactaaat aatgacatcc
      901 gcgagctgga actggaagaa caaaaagaga ttgaagtgat cctttccacc ctgagccagc
      961 aaactgcaga acagaccgac tctatccgcg cagatcttaa tatcatggta cagcttgacg
     1021 tcatttttgc cagagcatcc cttgccatgg atatgaatgc aacagaacca atcttcaatg
     1081 atgaaggacg catccgtctg aaacaggcac gccaccctct cattgataag aagaaagctg
     1141 ttcccattga tatccgtctc ggcgatgatt tcgacttgct tgttatcacc ggtccgaaca
     1201 ccggtggtaa aactgtatct ttaaagactg tcggacttct cactctcatg ggccagtccg
     1261 gtcttcatat tcccacactg gaccgctcag agcttgcact gtttgaagaa gtttatgctg
     1321 atatcggtga tgaacagagt attgaacagt cattaagtac attctcctca catatgacca
     1381 acgtggtttc tttcctgaaa aaagccaacc gtcactctct tgtccttttc gatgagcttg
     1441 gtgcaggtac agatccgaca gagggtgctg cccttgccat tgcgatcctt tcccatcttc
     1501 atgaacaggg aatccgcact atggcaacca cccattacag tgagctgaag gtatatgcac
     1561 tctccacctc aggtgtagaa aatgcctgct gcgaatttga cgtggaaacg ctccgcccga
     1621 cctaccgcct gctgatcggt gttcccggga aaagtaatgc attcgccatt tcttccaagc
     1681 ttggacttcc ggactatatc atcgataagg ccaaagaaca gatcagtgaa caggatgaat
     1741 ctttcgaaga tgttctcagt tccctggaaa gcagccgtat aactattgaa aatgaacgcc
     1801 gcgagattga acaatataaa caggaaattg cctctctgaa atccgaaatg gaatccaagc
     1861 aggagaagtt gaacgagcag cgagaccgca tcatccgtca ggccaatgag gaagctcatg
     1921 cagttctccg cgaagccaag gaatatgcag accagactat gaagatgttc cacaaattcc
     1981 agaaagacca tgtggatctc tctgctgtgg aaaaagaacg ccagaatctg cgcaaacata
     2041 tgaataaagc agagaaaggc atgactcaga aaactgctgc aaagaaaccg aagaaggaac
     2101 tcacagccaa agatatctcc ctcggagatg cagttaaggt tctcagcatg aatctgaaag
     2161 gtactgtcag cagccgtccg gacaacaaag gtttcctctt cgttcagatg ggtatcatcc
     2221 gttccaaggt tcacatctct gatctggaac tgatcgatga agcagagatc accacaccaa
     2281 ccatgcagcg tacaggtgca ggtaaaatcc gcatgtcaaa agcagcccat gtatctacag
     2341 agatcaacct gcttggcaaa acggtagatg aagcagttgc agagcttgac aaatatctgg
     2401 atgatgctta tatcgctcat ctgaaatctg tccgtattgt tcatggtaaa ggaaccggtg
     2461 ctctaagaaa aggtgtacac aattatctga agcgccagaa gcatgtggaa tccttccgcc
     2521 ttggagaatt cggcgaagga gatgcaggtg ttactattgt agaatttaag aaataggagg
     2581 gaccgccatg gtttccaaac agaaaatcct tattgttgat gatgataaca atatcgcaga
     2641 gcttattgcc ctgtatctta ccaaagaatg cttcgagaca aaaatcgtaa atgacggaga
     2701 agaagcgtta agggaatttg cctccttccg cccggatctg atcattctgg atctgatgct
     2761 tccgggaatt gacggttatc aggtatgtcg tgaaatccga catacctctg atgttcccat
     2821 cattatgctt tccgcaaaag gcgaaacctt tgacaaggtt ctcggacttg aactgggtgc
     2881 agatgactat atgatcaaac cctttgattc caaggaactg gttgcccgtg tacgtgctgt
     2941 cctgcgccgt ttccaggtta aacagccatc gccttcttcc agtgaaaaat gtgttactta
     3001 cccggatctg accgttaatc tgacaaacta tgcagtcaca tatatgggtc gtcaggttga
     3061 tatgccgcca aaggaactgg aacttctcta tttccttgca gcatcaccaa accaggtatt
     3121 tacccgtgaa cagcttctgg atcatatctg gggttacgaa tacatcggtg atacccgtac
     3181 tgtagacgtt catatcaaac gtcttcgtga aaagatcaaa gacaatcctc actggtccat
     3241 tgccaccgta tggggaatcg gatacaaatt tgaggtaaaa aatccatgaa acagcatcat
     3301 ctttccacaa aattcctgag attttatatt ctcatcggga ttttaggatt tttcctcata
     3361 accctcggcg gttcttatat ggtggaaaag catctggaac actctttaag tgccgcactt
     3421 tatacagaag cgcacaatat tgcatctaac gaagcagtta aaagcaatat ctcttccagt
     3481 acagtagata ctcttcagga acacttatgt gcaatttccg attttcagga tgctgtattg
     3541 tggatcatca acagtaacgg tgaaatcatt gtaagtacac agaaaaatat ttacgtccgg
     3601 gagccaatcc cactggaaga attcgatgct tccaaatggg gaagcaacta ttatcagatt
     3661 ggaaagtttt atggtttctt taaaacagat catttgagtg ttattgcccc gatcaccttt
     3721 gatatggaaa ccaaaggtta cgtggcaatc cattattcga tgacaaacct ttatcagagc
     3781 cgaagcagta ttctttttat catgcaggta atctttctcc tgtgctatgc agccaccagt
     3841 cttctgctct gggcttacag ccactacata cgtaagcccc tggccaggat catgaaaggt
     3901 gccagtgaat atgcaggagg taatcttgca tataaaatag acgttacttc tgatgatgaa
     3961 atgggatatc ttgccaaaac tctgaattat atgtccgatg aactgaacaa aaacggagaa
     4021 tatcagcgta aattcattgc aaatgtctct catgatttcc gttctcccct tacctccatc
     4081 aagggatatg tcaatgccat actggacggt accatcccct atgagatgca ggaacggtat
     4141 ctgaagatca tcgcttttga atctgaacgt cttgaaaaac tcacccgcag tcttctgact
     4201 ctgaatgaac tggatatgaa aaaacggatg atgcacattc agcgttttga tatcaatgat
     4261 accatcaaaa acacagcagc tacatttgaa ggcatctgca ccagccgcca gatccgtctg
     4321 gagctgcttc tctccggaca tgaactttat gtcagagctg atatggaaca aattcagcag
     4381 gttctttaca accttcttga taatgccatt aaattcagca atgataattc ctctgttcag
     4441 attgaaacaa ctgtaaaaag cggtaaggtc ttcgtttctg taaaagatta cggaaccggc
     4501 attcccaaag aaagcctggg caaaatctgg gatcgtttct acaaaattga tgcatcccgc
     4561 ggaaaagacc gtaaaggcac cggacttgga ctttctatag tcaaagagat catcaatgca
     4621 cataaccaga atattgatgt gatcagtaca gaaggtgtgg gaaccgaatt tatctttact
     4681 ctggagaaaa cgaaataaat ttctttcact tgaaaaatat tgtcttaaat atacctgtaa
     4741 caaccctctt acctggagcg tgtctgagag gactgctata aaaaataccg taacaaaagt
     4801 atatatcatt tacttttatt acggtatttt ttttattcta tggcaatcct cgtaaataat
     4861 gcatttaaga catttcaccc accttaacta aacggtgttc tcaacattct gtcttcattt
     4921 acgagataga ttcctctcgg tgtgatcctg atttcaggaa tcacaggaag tgccataaat
     4981 gacaaggtga taaacggatc aaatccttct ttcactccca tctcatgggc cttcggaatc
     5041 atttttgcaa gtgcttcgtt tacactctca tagccggcat cgctcatcag tcccatgaca
     5101 ggaagtggta aggttccata aatctctcca ttgcatacca gagtataacc accttgcatc
     5161 cgcatcattt cttttacagc aatagccata tcacgatcat tatctcctac tacaataata
     5221 ttgtgggaat catgagaaac actggaagca atggctccgc cgcggattcc atagcctttt
     5281 acaatgccca ctcccatctt gcccgtattc ttatgacgct cgatcactgc aatcttctga
     5341 tattccccgt ccggagcaaa atatttctca ccatccgatt ctatccatgg aacctcttcc
     5401 agaacatccc ttgtcacaat ctgtttctca agcatctgaa tcactcttgc ctttgttccc
     5461 ggatgtttca gtttcagacg ttcctcactg aaacctgata catgaactgt atttttcaga
     5521 tatggaggac atggtttcaa ttctgctttc tcatctttaa tgatcttctt accacaatga
     5581 tatacatcta caacattcag atctgccaca ttatccagaa tgacaaaatc tgcctgtctt
     5641 ccgggtgcga tcattccaag tctgtataaa ccataacatc ttgcaggctg gatcgttgcc
     5701 atctgcagtg ctttctcaac aggaagtcca agctgtactg cacgctttac attataattg
     5761 atatgcccct ctttacggat ttcttcaata tgtttatcat cagtacagaa gcagaaacct
     5821 gacgtatctg tatggtgttc cacaatcccc ttcacaatag catccagatt tcttgctgca
     5881 cttccctcac gtatcagtac ctgcattcca tttcttgcct cagccattgc atactcataa
     5941 tccacacact catgatctgt atcaattccg ccaagtacat atgcagccag atctccaggt
     6001 gccagaaacg gtgcatgacc atccttcact ctgtcctgaa acagctgaag cttttcatgc
     6061 atggcaacac ttccgttaat cacagaaggc gcatccatca cttcacccag ccccagaatc
     6121 ctcggatgtt ccagatatcc cttcatcttt cctgctgtaa gaatacaccc gttatcatcc
     6181 acatgagtcg ccggaacaca ggaaggcatc attacatata cattcgccgg tgcatcttct
     6241 gtctgatcca ggatataatc aattccatcc gtaccggata catttgcaga ctcatgagga
     6301 tctacaataa atgttgtagt tccacactgt gcagcctgcc ggaccagctc acccggcgtc
     6361 accagagtag attccagatg cagatgactg tcgataaatc ccggacagat tacttttcct
     6421 tccagatcaa tctctgtctc tccttcgtat gtaccgactc cgatcacaat tccatctgca
     6481 acagccacat caccatcaat aaattctcct gtaccagagc tgaacacacg cccattttta
     6541 aacaccagct cagccttctc aatacctctt gccatctgcg aatacctgcg atacatctca
     6601 agttccataa aaaaacctcc cttcacaccc ttgaaagccc tcctgcgcca cacattcatt
     6661 attacagtaa agcttccaat atattatcat atttacttgt tttttcagac ttttttattg
     6721 tcccaatcat ttttttctat atatttcatt atatattatc atctgtcttt ttaatttgca
     6781 acctgttttt tctttaccaa agcaactttc catcaacttt taacggcttt acaaaaaggc
     6841 tgccggatat ctcctgcagc cttttcatat cacttacatc tctatattta catcatataa
     6901 tctttgatca catcatgatc tctgagtact ttctcgacca cagttccctt aacagctttc
     6961 aaaagcggtt tcttcagcat atccagtggt cccggaagtt cgatctccag tggagatttt
     7021 ccatccatca gcttcacact gctatccagt aattctctga tatcataaac actgccccat
     7081 acttccggca cacctctgtc aacaccaaga agtccatata cagcctccat agcagttctt
     7141 acggaatatt cagtagtaaa tacagtatct cttggtgtat cggcaaactg tccgaggaat
     7201 gcgaagttta cacagccatc cggaataaca tcaggtctgt caccctttgt tctcggcata
     7261 aagaatgctg taatataagg catcatggtc ggtacacaaa ctgcactgtt ctcagccatg
     7321 tcttcaatct ggtcaaccgg aacaccaaga tgatagagcc actcttctgt gatttcctta
     7381 cctgtacact ccttcatagg cttcttgata taatcaccag gtacatctgt aaacagacca
     7441 tatacccata cacaaacctt gtctttgtcc tgatccttga actgtccctg tctgttgatc
     7501 gtccagctta ataaccagct ggaatcctga cagcttacaa taccgcctgt aacaaccttg
     7561 cctgttctcg gatcacgttt gcagatatct gtaatataag gaatgatctt atcatccaga
     7621 gtagtcactg ttgctgactc ccaatttgtc ttagcaatat cagaacagaa tttctcagga
     7681 tgtccgaagg acggatcctg ttttgcgata tttttccaga gactccagca tccacttgta
     7741 cgaacttctg catctccgtt cggtgcatga ttctgatcgc catagatggt tccctctgta
     7801 cagcttccgt ttgtcacaaa tacaaagtcg ttttctgtaa gaacaattcc cttttctacg
     7861 ccattcacct tacactcgat tgcttttgca atcttcttat tgccttcaaa ctcaaagagt
     7921 acgtttgtaa cctctgtatt gaactggaat tcaacgcctg cttcttccag atatttctgc
     7981 attggaagaa tcagggattc atactgattg tatttagtaa atttcagggc gctgaaatcc
     8041 ggaagtccgg aaatgtgatg gataaatctc tggaagtaca gtttcatttc cagtgcactg
     8101 tgccagttct caaatgcgaa catggttctc cagtataacc agaatgtgga atcaaatact
     8161 tcatcgtcaa atacatcttc aattgtcttg tcataaagat cttcatcctt tgtcatgaac
     8221 agcttcatga tctccatgca gcccttctgg ctcagattga atttaccgtc tgtatgagca
     8281 tccttgcctc tctcttttgt tgcacgacag agagaatagt tggggtcttc cttattcagc
     8341 cagtaatatt catcaagtac agatacacca ggtgtttcaa tggatggaat acttctgaaa
     8401 agatcccata agcactcgaa atggttttcc atctcacgtc ccccacgcat cacatatccg
     8461 cggctcggat caaagatacc gtcacatgca ccgcctgcaa tatccattgc ttccagaatg
     8521 tggatattct ttcctggcat ctgtccgtca cgtacaagga aacaggctgc tgcaagagaa
     8581 gccagtccgc ttcctacaat ataagcatgt ttgtcatcta cgccttccgg ctttcttgga
     8641 cgtgcaaatg cttcatagtt accgtttgta taataaattc ccttactgtt tttctcatat
     8701 tttccacgtt ctgtattgcg atattcactt gtattatgaa gtgcgtcata atcttctttt
     8761 ttctttgtct cagctgtttt atacttcttt cctgctgcca ctgctgccac gcctgctcca
     8821 actgccaatg cggatacaag tcctaaacct ttatttttac tcataataca catccacctt
     8881 tcttcaattc ttctttagaa gttctttcag ctctgtgatt ttaaatgctt ttcgttctct
     8941 ctgctttacc catttcccgg caagacctct tcatgacttt tacattctta tctgtatatt
     9001 tcccatatca taagcatttc atccgtctga attatctgtt tgttgctatg gctgtatatt
     9061 aacagtaaaa atatgattct acaatttaca aaagccgccg aatgataagt tttttatcac
     9121 tctgcggctt ttgtaaagaa tttttacatg attatatttc tgtatttttt tccataatat
     9181 ttttctgaaa gttatgaatg gatgtagtca ctgtatcgtg aagcgtcaga tccatcattc
     9241 ttaccagatc ttcaatattt tctttcattc cttccctgat ccactcaagc atgattccca
     9301 caaaaccata tttatagaag tctgcaataa attttttatc ctcttctgtg atattcagac
     9361 ccttgctttt ttcttccacc acacccacga tcagatcata ggttaattta aaaagataat
     9421 tttctatccg ctccctgtcc acatttctgt aaacattcat aataaacggt ttgttttcca
     9481 gcactgcccc aaatatctgg gtcagccctt ctgtccagga ctcagaagtc tttttgccct
     9541 gaagggcttt tgtcccatct tccacacagg accattcaac cagatcataa atatccttaa
     9601 aatgataata aaaagccatg cggcttattc cacagtctgt ggtaatatct gttatcgtga
     9661 tcttgtccag aggtttcttc agaagaagcc gtttcaggga ttcttccaga gctgcttttg
     9721 tggtatttgg catataactc ctccagtctg cttactgtga ttatttccag tcgtagtttc
     9781 tcaggtgtcc ttccagattc atgtggtcaa aattattctg gcgcagtgcc tcgtacagaa
     9841 caattgctac ggaattgctc aggttcagag aacggatatc tccgatcata ggaatcctta
     9901 cgcaggtttc ctgattattg accaaaatgt cctccgggat tcctgcactc tcttttccaa
     9961 acataatgta acagtcttct tcataatgaa catcagtata agtctgaggt gcttttgttg
    10021 ttgcatagta aatctttgcc ccgggattct tctccagaaa gtcctcaaag tccagatatg
    10081 tagttacatc caggtctttc cagtaatcca ttcccgctct ctttaaggct ttctcactta
    10141 aggaaaatcc cagaggctca ataagatgga gtctggttcc tgttgccaca caggtacggc
    10201 cgatatttcc tgtgtttgca ggaatctccg gctcatgaag tacaatattt aattttgcca
    10261 ttttattttt attcctcttg ttattaatcg tctgtctttt cttagaaaat tgtctcttaa
    10321 gtttcctgca gttactcttt gagaagttgc tgcagttcat cctctgccgg acgttccaga
    10381 tatcgggcac tgccgccact gtagagaagc cggtcattgc cactgtttgt ctgaccgatg
    10441 acctctgcca taaatccctc attgcgaagt ctctgtacca gtgcttcacc tccgcggatt
    10501 ccaatcaata ctgctccatc tgcctgaagc ttatatggat tcaggtcaaa aatctcgcac
    10561 acttctatgg tctcctgacg aatcggtacc ttacggaagt ctgcttccag tcctacttct
    10621 gaagcttctg ccatcttcca gagcgcactt aaaaaaccgc cctcacccat agcatataat
    10681 gcgctggcat ctgcctcctg cgcaattttc cagacaatac tgccggctcc ataagcatcc
    10741 caaagtgaaa cgcaattctg aataaaaccg gcagagaatc gctctgccag tttgtccttt
    10801 ttattttttg ctattacaga ggttcctttc aaagccaccg ggcatgccac taccagttca
    10861 tctcccggtt tcaggcatcc ggttatttct gccctcagag cctccattgc cgcctgtccc
    10921 agtctcattc ttttcctccg aaaatttatt atacgatcag agacagaact gtcggcacta
    10981 ccattgccag taccagaatg attgcaataa ttgctgtgat cactttacgg ttctttggat
    11041 taaacattcc catttctctt cacctcttta atctctgttc aggttctcaa acacatcttt
    11101 cgtcacatct gtcacatgag cattgttagt cagcggatca cggtctgcat agttaaacaa
    11161 cagcactgtt ccatctgcaa atacttccac atgtgcattc tttgccactt tttcccgttt
    11221 tctgaagtcc catacctgtc tctcaaaagg cttctgatct ctggcaaagc caggacggct
    11281 tttcaggttt tctctcagat agagatcata taccatctgg tcttttacct gatccatgga
    11341 aatctccggc acatcctcaa gaaattccag caggatctca tatcttcgca ttcttgtatg
    11401 agaaacatca ccatagccct tttccatgta aaagctgccc agtctctcat aaatcgagaa
    11461 agcatcctgg aaaaagtttt ccagatattc aagtgtattc tggaattgtc cactattata
    11521 atacacttcc accatatttt ccacagtttt cagcttcaaa acatggtcat aatccagcca
    11581 cttcgtggaa agtacctcat aaggctcctg tgttttgtat acaataccat attccctgca
    11641 catctccatc atatgggaac cctttaacac cttcaaaaag cccagctgaa gctgctgtgg
    11701 tttcagtgca taaacatcat taaaggaatg ccggaagctg tcgtaatctt catagggaag
    11761 tcctgcaatc agatccagat gctgatggat atttccaaag ctatggattc tgtcaacaat
    11821 tcctttcagt ttttcaaaat ccatagttct gtgaattgcc ttaatcgtgt ccgggtttgt
    11881 tgactgtact ccgatctcca gctgaatcag acccgggcgc atggtggaca tttcctgcag
    11941 ttcctcttct cttaacagat ctgctgaaat ctcaaaatgg aagtttgtca caccattatc
    12001 gtgttcatta atatacttcc agattgccat tgcatggtca tgtttacagt taaaggtacg
    12061 gtctacaaac tttacctgcg gaaccttatt gtctataaaa aactgcagct ctttctttac
    12121 tgtctctgta tctctgaaac gcagtttctt atcaatggaa gaaagacagt aactgcagga
    12181 gaaaggacat cctctgctgc tttcataata aatgatcctg ttcttaaact cagacagatc
    12241 tttgtaaatg aacggaatac tgcttaagtc catgatctgg cgccatccat tatgtatgat
    12301 ctgatctccg tctctgtaac agattcctgt catatctttc agatcctgcg gatttttttc
    12361 cacataatat ccggcaagtt ctttaaatgt ttcttcacct tccccgacca taacaccttt
    12421 aaattctgag ttttctgtca ggaatttctc cgcatcataa gaaacttccg gaccgccggc
    12481 ccagaagtcg gcgcccggaa gaatctttat cagatctgcc atcagttctt ttacaaaaga
    12541 aaggttccag atataacagg acacgcatac cacatcagga tgctccagat aaatatcacg
    12601 catgatatca tctttctgct ggttgatggt atattccttt aatacaatat gatctgcgta
    12661 atcagatgca tatgcctgca gatcataaac agcaagatta gaatgaatat attttgcatt
    12721 gcatgccact aataaaatct tcataaatct ttagccttcc agtgcactct tcagctcttc
    12781 tttggaagta agacctacga agcgtttcac ttccgcaccg tctttaaaca ggatcagtgt
    12841 cggaatgctg actacacgaa actgctgtgc aagtgccata ttctgatcta cgtctacttt
    12901 tcctactgcg tatccctctt ctgccagttc ttccacaacc ggaccctgac gcatacatgg
    12961 accacaccat gttgcccaga aatctataag aaccggtttc tcagatttta atacttctgt
    13021 ttcgaagttt gctgttgtaa tctcttttgc catgatttat atctcctttg cttattcttt
    13081 ataaaaattt gcaatgtaag ttctctggtt ttataatatc aacactataa ccctgttaaa
    13141 ttttcagttt ttattttaga tttttatttt aaagatctta catacttccc tgcattaata
    13201 cctgcctgtg ctccctcata gacagctttc gccacctgaa gaagtccgcc tgtacagtca
    13261 cctgccgcat agagtcccgg gatggttgtt tccatatttt cgttaacttt aatgttgcct
    13321 ttttcagtaa gttcagctcc catctggcgg gcaatctcag tactgcctgc agttcccatt
    13381 gcaataaata caccctctgc aggacagaaa tcatttgcct gctgacctgc tcctgccttt
    13441 atttcctctg cctgcacatc aggttcaaga cggattccgg ataccagatc atcgccctct
    13501 acagactgga tcttcattgt gtttacactg atattttctt caggtgtaaa ttccggttct
    13561 tcaccgtttg tataaatggt tactgtagaa gcagtattgg acaattcctt tgcttcatgc
    13621 atggcgaaat caccgtttcc cagaactgct acatccttgc ctctgtaaaa gaaagcatcg
    13681 cagattgcac agtagctgac accttttccc tcaaattctt taacacccgg gatcttcggt
    13741 gcagcgcgct tacttcctgt tgcaagaatc agactctgag tctcgaattc accttctgta
    13801 gtttttacca caaaagtatc aaacccgccg actcccagaa cctgtgcatc caatattcgt
    13861 acgcccagtg ctttcgcctg agcgattcct gtatcatata attcctgacc ggacagcgga
    13921 tgttccagtc cgtaatagtt ttctattttc tctgcttttt ccaaagcacc gatcccatta
    13981 ttgatcacca ggggatccag atttccacga actgcataaa gcgcagcgga aattcctgca
    14041 ggtccggcac ctatgattat cactttttcc aaattctgtc acctgctctc tgttattgtt
    14101 ttcaatatac tgtgatatat catcgttact gttcactctg ttctcagtaa cttattatca
    14161 gctattgtta ctatactacg ttttctgaaa aaaatccatg attttatttg gataattttg
    14221 gtataatttg gttaaataag tttctcgatt cagcgacata ataaataaaa gcgaagtgct
    14281 gttcactcat gaaccatgca tttcgctttt tacattattt tgtatgattt atttatccaa
    14341 ccaggggata tttgtctgta agttctttga cgattgcctt tgccttttcc tggctgcctt
    14401 cttcttttaa ggtcatagca attgcttctg cgatcttgtc catatcttcc ggtttcatgc
    14461 ctcgtgaagt aactgctgct gttccgagac gtacaccgct tgtaacaaat ggagactggg
    14521 gatcatttgg aattgcattc ttattgcagg taatgtttac ttcatcaaga agattctcca
    14581 tctgcttgcc tgtaacgccc agacttctga ggtctacaag catcagatga ttgtctgtac
    14641 caccggaaac gatatcaatt cctcgtttct ggagtccttt gcaaagtgcc tgcgcattct
    14701 caacgatcat ctttgcatag tccttaaatt ccggctgaag cgcttctttg aaacatactg
    14761 ccttggcagc gatcacgtgc atcagaggac caccctggat tcccggaaat actgctttat
    14821 taaagttaaa tttctttgca ttctctgcgc tgcttaagat caggccgcct cttggtccac
    14881 gcagagtttt gtgtgtggtt gtagtcacaa cgtctgcata aggaatcggg cttggatgct
    14941 gtcctcctgc taccagaccg gcgatatgcg ccatatccac catcagataa gccccaactt
    15001 catctgcaat ctcacggaat ttcttaaaat caataactct ggcataggca cttgcacctg
    15061 ctacgatcag ctttggttta ttctcaattg caattcttct gacttcttca taatcgatca
    15121 caccgtcatc attgactcca taatataccg gcttaaagta ggttccggac atatttgcag
    15181 gacttccatg tgtcagatgt ccaccatgat ccagattcat acccattaca gtatctcccg
    15241 gctgcagcat tgcaaaaaag actgccatat ttgcctgtgc tccggagtgt ggctgtacat
    15301 ttgcatattc acatccgaat aattctttcg cacgttcgat tgcaagagct tccacctcat
    15361 ccacgcagga acatccgccg taaaatctct tccccggata tccttccgca tatttattag
    15421 taagagggct tcccattgct gccataactg ctttacttac ccagttctct gaagcgatca
    15481 gctcaatgtg ggaattctgg cgtgcaagct ccgcctcgat cagatctgcg atatccttgt
    15541 ctgtctttgc tacgtcgtcg attgaataca tcttcgtgct cctcctttgt tatacgctca
    15601 gtaattattc cgattataac caattctatc catcatgtca atcaccaaaa gacatttgtc
    15661 cgcataccaa gatattttat gatataaatt aggatactac atattatgtt tctctgctga
    15721 gctctgtagc aaataacaga ttcgattcca tcaaaacgct ttttttatct gttttaccag
    15781 ttcatccaca tcttccgctt tcacaagaaa tctctcggtt ccattgattt ccagactatc
    15841 ataagtatca tcatatgcaa aatatttcag agttacttcc ggagcttctt ctgtgtttct
    15901 gtggaatgtt acagtcagca cttcttcctt atctgctgca tcttttgttt cttcgacttc
    15961 cgaatcaagc attatacttt gaagcgcctg atacaattca gtaaatttct ctttctttac
    16021 agttttcttt ccgaatttat aatcgctgcc gtccagcttc atcgtatagg ttttctttcc
    16081 aatgctcata tccacactgt caagtgtatc aatatttacc aatgccggaa tcttcagaat
    16141 caaattaaat ggtttcagtt ccagaagact gttcactgac tcttttgaaa gcagataaac
    16201 agcctcttcg tatcctttta cacatgcata atagtcacca ttctcatctg tatttccgat
    16261 aagaattgtt gctgttgcat ctgcatcctg cgtttctttt gctgtgtcat cgttaactgt
    16321 atttacaaaa tctactgtaa aatatgtctt tgtattatcc agtccggtat ctgtatttgc
    16381 tgcatcgacg ccgttgctga gatcaaaagc agccagcact ccaaacattt catacatatt
    16441 ctctgtgttt actgttgcaa tctcatcata cggattgtta atttcccagt agtcgaaatc
    16501 catcttataa tctgccggtt cctgtttgat ttgaaatact tcttcgccgc cctttacaat
    16561 ggagaatctg cggatagaat agacagacat tcttggatgt tctacagttt ttaaatctgt
    16621 attttctgtt ttatcatctg tctgatcagc aatttcttct tccacagctg catcttctgc
    16681 ttttggctct tctgcagtct cagagtcttg ttctgcttca tctgcccata caggacagga
    16741 tataactgct gtgcctgctg caccggcagt aaacatggct gtcattccaa gaagtaatat
    16801 ttttttaatt ttgtttttca tgtctgtgtt cctcctgtcc atatataata tggtttttat
    16861 tttatgttac ttttcgcaat tgaaacagaa tacttactcc gttcggttaa agtgcaattt
    16921 ctacctcagc aggcttatgt cctgtcagtt ctgcacttct tgtatccggc tgggatactc
    16981 ctgcaaataa acggaaatgt tttcctgcga tgtaacgatt tccatcttca tcaactatat
    17041 tcattgcttt attagaaatt gtaaattcta cagatttctc ttctcccgct ttcagagata
    17101 ctctcttaaa tccgcagaga ctgtagttac gaactgcaag cggagaatca aggtctttga
    17161 tatacacctg aacaacttca tctgtatcta ctgttccatt gttcttaaca gttgctttta
    17221 acatgatatc ggactctgct gatacttcac cgacaacctc tgcttctgtt acgcaggtat
    17281 cactgtatgt aagtccgtat ccaaatggat aaagtgcctc tttttccata taacggtaag
    17341 ttctgttttt catagagtaa tctgtgaatt ccggcattcc ttccaggtct ttataaaaag
    17401 taaccggaag ttttcctgac ggagattctt ttccgaagag aagatctgct attgcacgtc
    17461 cgcctcttgc tcccggatac catgcaagaa ggattccatt acagttatcc tgcgcataat
    17521 tcaggtcaat cgcacttcct gccatcagaa ctacgatggt aggttttcct actgctgtta
    17581 ctttctcgat cagttcttcc tgtactttcg gaagatgaag atcaactttg tccccggatg
    17641 catcactgtt tccggtatct ccttcttctc cctccagagt ctcatcaaga cctacgcaga
    17701 gtatgactac atcgctgttt tccgctgtaa ttactgcctc agaaattctg tcctgatccc
    17761 atgccagatt ttcaactttg tctttatata aatcacatcc ctgagagtaa aggatcctta
    17821 catcgtcacc tgcttcttca cggattccct caagaacagt gacatattca gaagatgttc
    17881 catgataatt tccgatgaga gcggcacggc tgtctgcatt cggtccgatc acaccgatag
    17941 tatttacttt agatttatca atcggaagaa ctccgtcatt cttcaagagt acgcagctct
    18001 ttcttgccat cttaagagct tcatcaatgt gctctttgca ctctaccaca ctatacggta
    18061 tcttatcata ttcacttccg tcaaacaggc ccagcagata tctggttgtc agcagtctta
    18121 ctgcggattc tgtaattttc tcttctgtta ccagtccatc ctgataagct cccatcatat
    18181 gaagatatgt atttccacag ttcaggtcac agcctgcatt taatgccata gcagcggact
    18241 gtcttggtgt ggaagttacc atgtgatgtt catggaagtc acggattgcc cagcagtcag
    18301 aagtataatg accttcgaat ttccattttc ctctcaggac atcttccata agatatttat
    18361 gtgcacagca aggttctccg tttgttctgt tataggcacc cattacagct tctacatctg
    18421 cttctgtaac cagtgcttca aaagcaggaa ggtatgtttc ccacatatcc ttcatactta
    18481 cttccgcatc aaattcatgt cgcagagatt ccgggccact gtgtactgca aaatgttttg
    18541 cgcaggcagc agctttcata acagatccat caccctgaag tccttctacg aatttcacgc
    18601 caagtctgga cgtcagatat ggatcctcac cataggtttc atgccctctg ccccatctcg
    18661 gatctctgaa aatgtttaca ttgggggacc agaaagtaag acccttgtaa atgtctctgt
    18721 cttcttttgc agaatatgca ttgtattttg ctcttccctc agttgcaatg atatcgccaa
    18781 cttttttcat ctcttcatcg tcaaatactg cagccatcgc aattgcctgt gggaacatgg
    18841 ttgccacacc tgctctggcc actccatgca gtgcttcatt ccagtaattg tatgccggaa
    18901 cacccagtct tttcacggga gcagcatcgt attttaactg ggaagccttc tccagaagcg
    18961 tcatctgtga taccagtttt tctgcttttt ttcttgcttc tgctctttct tttctgtgat
    19021 ctttcatttc agatcctcct gtttatataa tttgataatt attcagcagg tattaacaaa
    19081 gcctcagcgc agcccgctac tcctgcatta tgtattctgc atttcttctc tgtatttcgt
    19141 c
//
LOCUS       DBMDXG010000004        18917 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_903_length_18917_cov_2.218856, whole genome shotgun sequence.
ACCESSION   DBMDXG010000004 DBMDXG010000000
VERSION     DBMDXG010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 18917)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18917)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18917
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_903_length_18917_cov_2.218856"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="ACZPD3_00345"
     CDS             <1..312
                     /locus_tag="ACZPD3_00345"
                     /inference="COORDINATES: protein motif:HMM:NF020122.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HIR1414502.1"
                     /translation="TVNQIGKGEVYYLGCDLDEKAMKMLAVYLGRKAGIDMDLYKVDG
                     VEVVDATDGTNDALFILNYNDHSVIVSMEQSYENMITHETVENVVELKPYDVAILKEA
                     N"
     gene            533..1663
                     /locus_tag="ACZPD3_00350"
     CDS             533..1663
                     /locus_tag="ACZPD3_00350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR1414503.1"
                     /translation="MGYVKWSYDTLNTFCHDVFRKFGFNEEETNIIKDVLLTADLYGI
                     ESHGMQRMVRYDKGIEKGTIHPDAKPEVVFETPVSAVIDGHDGMGQLISHFAMEKAIE
                     KAKKTGVGFVSVRNSNHFGIAGYYAEMASKQGLLGMACTNSEAIMVPTFGRKAMLGSN
                     PIAVAMPAEPYPFLFDCSTTVVTRGKLEMYNKMGKPLPQGWALGANGQESTDAPDVLA
                     NIVAKRGGGIMPLGGNKEVNGSHKGYGYGMLCEIFSSIFSMGVTSDKCCTFKDKTGIC
                     HGFAAIDPAIFGNADDIRAHFSEYLETLRQSPKAEGAEQIYTHGEKEVFAEKERRENG
                     IPVNDNTMVELANLCNYLKVDFDSYFKGYELPKDSKFFSGNY"
     gene            complement(1829..2275)
                     /locus_tag="ACZPD3_00355"
     CDS             complement(1829..2275)
                     /locus_tag="ACZPD3_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HIR1414504.1"
                     /translation="MSPKRQVNHNIRKHFNTIIYNISRYAEIALSMVILLVIALAGFR
                     LIMEVADTSVMSMDTEFFSTFLSQALSLVVGVEFVKMLCQHSAQTVVEVLMFATARQM
                     VVEHLGPAETLLGVLSIAVLFAIRKYLMTDNDDMNAHNCPKNTEEN"
     gene            complement(2352..3320)
                     /locus_tag="ACZPD3_00360"
     CDS             complement(2352..3320)
                     /locus_tag="ACZPD3_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR1414505.1"
                     /translation="MKKRKNIAIPMILAATIAAVSVPCSSQIVMASDTYQVGVSKGYL
                     ALRSAMDYDSSNEIGELYSGDTVEVTEYTTSDYWYVYSPKLNKSGYVNNDYLYFLSSQ
                     PTSSSGSYTVSVAKGYLALRSTKAYDSSNEIGQLYSGDTVTVSDSSDPQYWYVYSPKL
                     NLSGYVNKDYLYYSGDTAASAQSSSGDSRTVSVAKGYLALRSAKAYDSSNEIGQLYSG
                     DTVQLIDTSDSQYWYVYSQKLGKNGYVNKDYLIGGTTTYATRTVSVATGYLALRSAKA
                     YDSSNEIGQLYSGDTVQLVDTTDAQYWYIYSQKLCKYGYVNKDYLY"
     gene            complement(3367..4293)
                     /locus_tag="ACZPD3_00365"
     CDS             complement(3367..4293)
                     /locus_tag="ACZPD3_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024748.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR1414506.1"
                     /translation="MFSKKLLIPLLSLSLLVTPAYSSHAAENTEAVETSVEGQGMLSP
                     SSSSENSESVFNGSGAGTSSEPVTDEKLETILKQVQSQLPAENGTWAVFISDLVNGTE
                     GSLNDQKMQAASLIKLYIMGAVYENYDQITGQYGRDSVDSNLYSMITVSDNDAANTLT
                     TYLGGGDSAAGMQAVNSFCQAHGYDQTHMGRMLLASNENDDNYTSVGDCGHLLQEIYK
                     QDTSGYTHATDMFNLLKAQTRCNKIPAQLPEGVKTANKTGELDNVENDAGIIYDSKND
                     VVIVFMSQNLSSAGSAQNTIATLSRTIYDYYN"
     assembly_gap    4631..4640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4769..5803
                     /locus_tag="ACZPD3_00370"
     CDS             4769..5803
                     /locus_tag="ACZPD3_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR1414507.1"
                     /translation="MLTPKQNMLEVIKGGNPDRFVNQYEAVQLLFHPFMFANPLLQPG
                     QENVVNAWGVTNTFPKGVPGSFPVHTPDKIVVKDIEDWKDYVHAPSLKFTQDQWDMVK
                     AQYDAVDGEQAFKAAFVAPGLFEQTHHLCEISNALVYYITNPDEMHDLIKYLTEWELE
                     LAEGICSNLHPDALFHHDDWGGLDSTFMSPAMFDEFLLEPYKEIYGYYHSHGVELVIH
                     HSDSYAATLVPSMIEMGIDVWQGCMETNNLPELIRKYGGRISFMGGIENRAVDFEGWT
                     DENCDAVVRRVCEECGNKYFIPCIAQGGPGSVYPGVYKSLCDSIDKLNEEKFGIKASE
                     STRLPWQIMF"
     gene            5907..6725
                     /locus_tag="ACZPD3_00375"
     CDS             5907..6725
                     /locus_tag="ACZPD3_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HIR1414508.1"
                     /translation="MIIIGEKINGSIPIVAEAIAKRDSEFIKARAKLQAESGATYIDC
                     CASVPEAEEVETLKWMIDCIQEVTDLPISIDSPSPDVLAQAYKFCKKPGIFNSVSGEG
                     HKIDTIFPIMAEPGNEGWQVIALLSDNTGIPKCAADRLKVFDKIMAKAKEYGIAPDRI
                     HIDPLVEMLCTSEDGIATNIETITSVREQYPTIHITAAISNISFNLPVRKLINYGFLV
                     LAMNAGLDSGIMDPTNKDMLGLVYATETLLGLDDYCMEYIGAYREGLIGTTKKK"
     gene            6802..7437
                     /locus_tag="ACZPD3_00380"
     CDS             6802..7437
                     /locus_tag="ACZPD3_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR1414509.1"
                     /translation="MSKINEVAELVEKGKAKLVGPAVQEAIDEGDDPVAILNDGMISA
                     MSVVGEKFKNGEIFVPEMLVAARAMKKGVEVLKPHLASGSTGALGKVIIATVSGDLHD
                     IGKNLVAMMIESAGFEVIDLGVDVPAAKIIECYKENPDVKIIALSALLTTTMPALKET
                     VEALNAADFRGNIKVMVGGAPITEAFAEEIGADGYSDDAASAASLAKKLAA"
     gene            7821..9554
                     /locus_tag="ACZPD3_00385"
     CDS             7821..9554
                     /locus_tag="ACZPD3_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR1414510.1"
                     /translation="MAWVKFVREGIEIEVNAGMSVLEAEIRADLRPDAPCGGLGKCGK
                     CLVKINGEVVKACQVRICEGETCVVETLDRAGNEKILTDGFNREVVFEPGLRMAQVEL
                     EKAKTGEKRSDWQRLLDTLAETDGEVEPGQMEVDLKLAGELYGMRRDSDEWYVIYSRR
                     RILEMRKEAGRRCLAAFDIGTTTIAGYLLDGADGRTLAVESRMNPQAQYGADVIMRAN
                     YALEHGTEALSMCVRKAVNEMLGSLAEDAGIRREDVFQVCVVGNTCMHHLFLGISPAS
                     LVHAPYTPAVSERLVLNAGDYGLAVQERAELIMLSDIAGYVGADTCGCLLAIRQDQQE
                     EISLMIDIGTNGEMVLGNRERMVTCSTAAGPAFEGAKIECGMRGAAGAVDHVKYEEGK
                     WNYTTVGNKPAVGLCGSGLIDLVAGLLDAGMLDENGVLRSGQEKQGVFILVPPERGGN
                     ERGVYLTQKDLGEVQLAKAAIAAGIQMLMERLGITEDDICSVYIAGAFGNYMDPVSAG
                     KIGLLPATLVKKVKPVGNAAGEGAKIALVNEKEMLEMDELVRKIEFVELAASADFQDH
                     FIDELGFETGE"
     gene            9611..10147
                     /locus_tag="ACZPD3_00390"
     CDS             9611..10147
                     /locus_tag="ACZPD3_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HIR1414511.1"
                     /translation="MDIKKIEQMGMEQGFSHVVLLDCDTIELKPEVRQMCAADTCHKY
                     DKCWSCPPGCGSLEECEAKVRQYKYGIIVQTVGELEDVFDGEGMMETEARHKEYFVEF
                     EKKLREIYPDMLAIGAGCCTKCKVCTYPDAPCRFPKQAFSSMEAYGMLVTQVCQANEL
                     QYYYGDCTIAYTSCYLLE"
     gene            10612..11247
                     /locus_tag="ACZPD3_00395"
     CDS             10612..11247
                     /locus_tag="ACZPD3_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HIR1414512.1"
                     /translation="MTVLEQLRKTIEDGHPGETEKLVREALKQHIPAGRIVEEAMTPA
                     MRTVGENYKSNGADIIKILAAARSVRKGFELLEEQDSQFGRRNIGTVILGTVEGDLHD
                     VGKNLVAIMFRSAGFKVIDLGVDISEKQFLRAVKQNPDVSIVCISSLLSTSIPEMEQV
                     VKSLKRSCSKHKFKIMVGGGAVTEQLAKNMGADAYTENCIEAVEVAKTFIV"
     gene            11391..12974
                     /locus_tag="ACZPD3_00400"
     CDS             11391..12974
                     /locus_tag="ACZPD3_00400"
                     /inference="COORDINATES: protein motif:HMM:NF037653.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR1414513.1"
                     /translation="MRLSSVYLYEKIQQQYEIQEKGNLSGSDGYLRPFLRCGISKEGK
                     KSEDGFQTGHVYVIQGCDANECEPVNSKAENIFWIFCKRKDTVRAEENGETVGKGAYI
                     QVALDSLEEIAEFMNDVQEIFDAADEWERKIHDLMLEHAGMDRLLQVTSEFLQNPMTV
                     TGLDFTFVAEAGSEYLPPRARLYTDDGLNMEYVNALLQNETYRDMADTHEYVMFPAYI
                     SGCRSMNRNLFVDGKATHRLVLTECRSEITLRVICVLDILVEKLEYLLAHEAEEEDPD
                     RDMEQIFVRILSDRTADYMQVSRELSELGWSGNHEYMCLILQITYLNQQNLSTKAICR
                     YIKKKFEDSVSFLYQDEIVAFFDLTRLGKSQEEVAGKLVYFIRDTYLKAGYSRVMTGH
                     MNLRRQYVQAKTALDVGSRKKPYLWIHYFGQVALTYILEQATRRLPGTMICHEGLLEL
                     KKHDEENQTQYMETLRVYLEQHLSATQAARELFIHRSTFLYRLDRIKEILQSELDDPE
                     EIFYLELSFRLLEQEQEKE"
     gene            complement(13097..13693)
                     /locus_tag="ACZPD3_00405"
     CDS             complement(13097..13693)
                     /locus_tag="ACZPD3_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR1414514.1"
                     /translation="MNTLKAEKRTMDTKAKRLRREGYVTGNVFGREMEGSLPVKIEKS
                     AVEKLLKTNNKGSQIMLDVDGQSYDVLIKEIQFNPLKGQVDEIDFQALVSNEKVHSVA
                     EIVLVNHDKLEAGVLQQHLEEISFRALPSALVDKIMVDVGDMKVGDVIKVKDLAIAQD
                     KDVDLKTDLEAAVVSVAAVHVDPALEAEEEAAAEEEAK"
     gene            complement(13963..15078)
                     /gene="glgD"
                     /locus_tag="ACZPD3_00410"
     CDS             complement(13963..15078)
                     /gene="glgD"
                     /locus_tag="ACZPD3_00410"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HIR1414515.1"
                     /translation="MYKAFGIVSSSGRNIYVDGMQDYRPIGAFSFLGRYRVIDFPISN
                     MTNSDIDRIQVYINNKPRSVVEHVGTGRHYNINSKSGKLQLLFSEHNNDNDIYNTDIS
                     CYLDNMESLSRMYHPYVVIAPSYMVYSIDFDQFLHTHIDSGADVTLLYHAVDNAKEAY
                     LTCNVLGLNRQKGVESIEPNHGNKKNQYVYMDTCVMKTELFISLIKEAKNLSSMYTLT
                     DILNDKCETLDIRGVAHKGFFASITDFPSYYAANMALLNYKSAQELFHENWPIYTRTN
                     DSCPTQYFNTADVKNSVISNGCQIEGTVENSVIGRGCIIKKGAVVRNSVVLAEATVGE
                     GVHVENEVVDKWAKLVHKKEIVSPTEQPGYIRRNDTL"
     gene            complement(15080..16219)
                     /locus_tag="ACZPD3_00415"
     CDS             complement(15080..16219)
                     /locus_tag="ACZPD3_00415"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1414516.1"
                     /translation="MKQNSMLAMILAGGRGSRLHDLTNKVAKPAVSYGGKYRIVDFPL
                     SNCANSGVDVVGVLTQYESIQLNSYVAAGGRWGLDAKNSGVYVLPPREKADENLNVYR
                     GTADAISQNIDFIDKFDPEYVLVLSGDHIYKMNYDKMLAAHKEAKADATIAVIGVPMK
                     EASRFGIMNTDESGRIVEFEEKPEHPKSNLASMGIYIFTWKLLRKMLMADIKNPDSSH
                     DFGKDIIPTMLNDNRTLYAYKFEGYWKDVGTIDSLWEANMDLLSSKNELDLGDPSWKI
                     YTEDVAALPQYISAEADVKDAYITQGCVVQGEVKHSVLFTGVKIGAGARIIDSVLMPG
                     VVVEEGAVVQRALVADGIRIGKGAVVGAADSEHIELVAKRVKGAE"
     gene            16661..18061
                     /locus_tag="ACZPD3_00420"
     CDS             16661..18061
                     /locus_tag="ACZPD3_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HIR1414517.1"
                     /translation="MSKREDYKRFIVFCLASLVVLAQAAVFAWVWYSVYRGQIDEPFW
                     RKGNWVLIAIYGLMFALFAKLYGGLKVGYLKRIDVFYSLTLALLCTNVVEYLEITLIN
                     RWFLSVWPMIEMTGIQLVLIIIWIFGSRYIYSGLYRARRLLVIYGDRDPGDDLIHKMN
                     SRKDKYDISGKVHISVGEEKIHQMMQDYDGVIIWDLHSTERNRYLKYCFAHSVRCYVS
                     PKISDIILMGSERIHLFDTPLLVSRNMGLAVDQRAAKRVMDILISGIGIIITSPIMLI
                     IAIAVKAYDRGPVFYFQDRLTLGGKPFKICKFRSMCVDSEKNGARLASKHDSRITPVG
                     HVLRNLHLDELPQLFNVFKGDMSLVGPRPERESIMLEYEKELPEFYYRLKVKAGLTGY
                     AQVYGKYNTTPYDKLKLDLFYIENYSFLLDIKLIFMTVKIFFQKEVSEGVDDRQVNAL
                     KDSGKNAGVSENKTEE"
     gene            18064..18828
                     /locus_tag="ACZPD3_00425"
     CDS             18064..18828
                     /locus_tag="ACZPD3_00425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1414518.1"
                     /translation="MNKPLVSVIMPVYNGEKYIRKAVESVYEQGVSLELLVIDDGSTD
                     HTEEVLSAYEGREDFRYIKNEQNMGAAGSRNRGVGLAQGTYIAFLDADDWWESGKLKE
                     QLKRLEETGYVLCSTGRELMKADGSSTGRTIPVKEKITYRELLKHNSINCSSVILRRD
                     VAREFPMEHDDSHEDYITWLKILRKYGCAAGINKPYLKYRLSEGGKSRNKLKSAAMTY
                     NVYRYAGYGRIRSCIFFCSYAVHGIWKYCYCSLRSQ"
BASE COUNT         5336 a         3324 c         4875 g         5372 t
ORIGIN      
        1 actgtaaacc agataggaaa aggtgaggta tattatttgg gctgcgatct ggatgaaaaa
       61 gccatgaaaa tgttggctgt ttatctggga agaaaagcag ggattgatat ggatttatat
      121 aaagttgatg gtgtagaagt tgtagatgct acagatggaa caaatgatgc attgtttatt
      181 ttgaactata atgaccattc agttatagtt tcaatggagc agtcatatga gaatatgatc
      241 acacatgaaa cagtggagaa tgttgttgaa ttaaaaccat atgatgttgc aattttaaaa
      301 gaagcaaatt aaaaaaataa tgaaggggag ctgctgtgaa gcaggcagtt ctagagcagg
      361 gtgagcaatt catttagaat tactcgccct gtttatatta tagaaaaaaa gaaaaataaa
      421 atcagaaaaa acagttgatt tttaaaagcg atggtaatat aatcatttta acaaattgat
      481 tggtataacc aatataagaa gaggtaatac tataaggagg ggctttgcag ttatgggata
      541 tgtaaaatgg agttatgaca cattaaatac tttttgccac gacgtatttc gtaaatttgg
      601 ttttaatgaa gaagagacga atatcatcaa ggatgttttg cttaccgcag atctgtacgg
      661 gattgaaagc catggaatgc agagaatggt aagatatgat aaaggaattg agaagggaac
      721 catccatccg gatgcgaaac ctgaggtcgt atttgagaca cctgtatcag cagtgatcga
      781 cggacatgac ggtatgggac agctgatttc ccattttgca atggagaaag ctattgagaa
      841 ggcgaagaaa acaggtgttg gatttgtatc tgtaagaaac tccaatcatt tcggtattgc
      901 aggatattat gcggaaatgg cttccaaaca gggcttactt ggtatggcat gtaccaactc
      961 ggaagcaatc atggtaccta cctttggacg aaaagcaatg cttggttcca atcccattgc
     1021 agtggcaatg ccggcagaac cttatccatt cctttttgac tgctccacaa cagtagttac
     1081 cagaggaaaa ctggaaatgt ataataagat gggaaaacca cttccacagg gctgggctct
     1141 gggagcaaat ggtcaggaaa gcacagatgc cccggatgta cttgccaata ttgttgccaa
     1201 gaggggcggc ggaatcatgc cacttggtgg taataaagag gtaaacggaa gtcataaggg
     1261 ttacggttat ggaatgctct gcgagatttt cagctccatc ttttccatgg gtgttacttc
     1321 tgataaatgc tgtacattta aagacaagac aggtatctgt catggatttg ctgccattga
     1381 tcccgcaatc ttcggaaatg cagatgatat cagggcacat ttttccgaat atctggaaac
     1441 tcttcgtcag agtccaaagg cagaaggcgc agagcagatt tatactcatg gtgagaagga
     1501 agtatttgca gaaaaagagc gcagagagaa tggcattcct gtaaatgaca acactatggt
     1561 tgagcttgcc aacctctgca attatctgaa ggttgatttt gactcttact tcaagggcta
     1621 tgaactgccg aaggacagta agttctttag tggtaattat taaataagcg cccatcatgc
     1681 tgataaccat aactatagaa tatatcatat aaaattactt tttttgagac gcccgtttat
     1741 cagcatgtgg aaataaaaag aaactgcata atcagatacg accggtagaa tttacagatc
     1801 gtatctgcta tgcagttttt tgttttcttt aattttcttc agtattcttc ggacagttat
     1861 gagcattcat gtcatcgttg tctgtcataa gatatttgcg gatcgcaaac agaactgcga
     1921 tactgagtac gccaagaaga gtttctgctg gcccgaggtg ttcgactacc atctggcgtg
     1981 ctgtggcaaa catcagaacc tcaacaactg tctgtgcgga atgctggcag agcattttta
     2041 caaattccac gcctactacc agagacaaag cctgggaaag gaaagtgctg aaaaattctg
     2101 tatccatgga cataacagaa gtatctgcaa cctccatgat cagccggaag cctgccagtg
     2161 cgatcaccag cagaattacc atagaaagag caatttccgc gtaacgtgaa atattgtaaa
     2221 tgattgtgtt aaagtgttta cgaatgttat ggttaacctg tcgtttgggg ctcataactg
     2281 tgagtccttt ctgttttgtt gtatatagtg aatgtaaaga agcgtaactg tttatagtgt
     2341 tacgaaaaag attaatataa ataatcttta tttacataac catatttgca gagtttctgg
     2401 gagtaaatat accagtactg tgcatcagtt gtatccacaa gctgaactgt atcgccggaa
     2461 tataactgtc cgatctcgtt ggaagagtcg taagccttcg cagagcgcag agccagataa
     2521 ccggtagcaa cactgactgt tctggtggca tatgtggttg ttccgccaat cagataatct
     2581 ttatttacat atccgttttt tcctaacttc tgagagtaaa cgtaccagta ctgggaatcg
     2641 ctggtgtcga taagctgaac tgtatcgccg gaatacagct gtccgatctc attggaggaa
     2701 tcataagctt ttgcggaacg cagagccaga taacccttgg caacactgac agtccttgaa
     2761 tcgccggaag aactctgtgc actggcagca gtatcgccac tgtaatataa gtaatcttta
     2821 tttacatagc cggataagtt cagtttcgga gaatagacat accagtactg aggatcactg
     2881 ctgtcagaaa ctgttactgt atcaccggag tataactgtc cgatctcgtt agaagagtca
     2941 taagcctttg tagaacgcag agccagataa cctttggcaa cactgacagt ataagaaccg
     3001 gacgaacttg tcggctgtga tgagagaaaa tacaggtaat cgttatttac gtatccggat
     3061 ttgttcagtt ttggagaata gacgtaccag taatcactgg tggtatattc tgtaacttca
     3121 acagtatcac cggaatacag ctcgccgatc tcgttggagg aatcataatc catggcagag
     3181 cgcagagcca gatacccttt acttacacct acctgataag tgtcagatgc cataacgatc
     3241 tgtgagctgc agggtacaga tacagcagca atcgtagctg caagaatcat gggaattgca
     3301 atattttttc tttttttcat agtgatcccc tcctttgggt tattatgtac tctgagagta
     3361 cattatttaa ttatagtaat cgtaaatcgt tctgcttaag gtagcgatcg tattctgtgc
     3421 ggaaccggca cttgagagat tctgggacat aaatacgatt actacgtcat ttttggaatc
     3481 atagatgatt ccggcatcat tttccacgtt gtcaagttct ccggttttat ttgcggtttt
     3541 gactccttcc ggaagctgtg cgggaatttt gttacagcgt gtctgtgcct tgagcagatt
     3601 aaacatatca gtggcatggg tgtagccgga tgtatcctgt ttataaatct cctgcaggag
     3661 atgaccacag tctcctacag atgtgtagtt atcatcattc tcattgctgg caaggagcat
     3721 acgtcccatg tgagtctggt catagccatg tgcctggcag aaactgttga cagcctgcat
     3781 acctgctgcg gaatcaccgc cgccaagata agttgtcagg gtattggctg catcgttatc
     3841 gctgactgtg atcatggagt acagattgga gtctacagaa tccctgccgt attgtccggt
     3901 tatctgatca taattctcgt agacagctcc catgatatag agcttgatca ggcttgcagc
     3961 ctgcattttc tgatcattaa gactgccctc ggttccattt acaagatcgg atatgaaaac
     4021 agcccaggtt ccgttttcag cgggaagctg actctgaacc tgttttaaaa ttgtctccag
     4081 tttctcatct gtgaccggtt ctgaagatgt gcctgccccg gaaccgttaa agacgctttc
     4141 agaattctct gaagatgaag aaggggaaag cattccctga ccttctacgg atgtttccac
     4201 ggcctctgta ttttcagcag catgactgct gtatgcagga gtaacaagaa ggctgagcga
     4261 tagcagaggt attaataatt tttttgaaaa cattgcgttc accacctgtt aaatatttac
     4321 tgtaaaacat tcggaatctg ttgatctgtt atataaatcg tatttcttta tatttagata
     4381 aatcaggttt aaaagtctta ttgtgcaggc ataaatgatt tttaaaatct gatatctgta
     4441 tatacaaaat tatacagtgt ttagagttgt gttacaagtg tatttacggt tgacttaaaa
     4501 tttattttgg aattgttata agtaagttcc tgtggaagtt gcagaaaatt cgtgatatat
     4561 cgtgtgaatt tatgggatag tataaatctg gcgtaataca tctgtcttgt gcatggtgta
     4621 tattgagatt nnnnnnnnnn tgattatatc ttttggattg tataaatcct acaataaaat
     4681 tatcagaaac gagacagaga acgacaaatt gaacaaacca atgtgagaat gtggggggat
     4741 tttcatattg catgaggagg tagattatat gttaacaccg aaacagaata tgctggaagt
     4801 tataaaaggc ggaaatcctg acagatttgt taatcagtat gaagcagtac agcttctttt
     4861 tcatccgttt atgtttgcaa acccgctgct tcagccgggt caggaaaatg tagtgaatgc
     4921 atggggggta accaatactt tcccgaaagg agttccgggt tcttttcctg tacatactcc
     4981 tgacaagatc gtggtaaagg atattgaaga ctggaaagac tatgtacacg caccgtcttt
     5041 gaaatttacc caggatcagt gggatatggt gaaggctcag tatgatgcag ttgacggaga
     5101 acaggcattt aaagcagctt tcgttgcacc gggattattt gagcagactc atcatctgtg
     5161 tgagatcagt aatgcgttgg tttattatat tactaatccg gatgaaatgc atgatctgat
     5221 caaatatctg acagagtggg aactggaact tgctgaaggt atctgttcca accttcatcc
     5281 tgatgcatta ttccatcatg atgactgggg tggactggac agtacattta tgagtcctgc
     5341 aatgtttgat gaatttttac ttgagccata taaagaaatt tacggatatt atcacagtca
     5401 tggcgttgag ctggttatcc atcattctga cagttacgca gcaacactgg ttccttctat
     5461 gattgagatg gggatcgatg tatggcaggg atgtatggaa accaataatc ttccggaact
     5521 gatcaggaaa tatggcggaa ggatcagctt catgggcggt atcgaaaatc gtgcagttga
     5581 ttttgagggc tggacagatg agaattgtga tgcagtggta cgtcgtgtct gcgaagaatg
     5641 tggaaacaaa tactttattc catgtatcgc tcagggtggc cctggttctg tttatccggg
     5701 tgtttataaa tctctctgtg attccattga caagctgaat gaagagaaat ttggcattaa
     5761 ggcatctgaa tctacaaggc ttccatggca gattatgttc tgatctcagg tatttgtcag
     5821 tggcaaaacg gaatacaaat atctgtccga catttagatg aaatttcagg tgttgcagag
     5881 gatgatgcta tggaggtgtt tgaaaaatga ttattattgg tgagaaaatt aatggttcta
     5941 tcccgatcgt tgcagaagcg atcgcaaaaa gagactctga atttattaaa gcaagagcaa
     6001 agcttcaggc ggaaagcgga gctacttata ttgactgttg cgcttctgtt ccggaagcgg
     6061 aagaagtgga aacattgaaa tggatgatcg actgtattca ggaggttaca gatcttccga
     6121 tctcaattga cagcccgagt ccggatgttc tggcacaggc ttataagttc tgtaagaaac
     6181 ctggaatttt taactctgta tcaggagagg gacataagat tgatacgatt ttcccgatca
     6241 tggctgagcc gggaaatgag ggatggcagg taattgcgct gctgagtgac aatacaggta
     6301 ttccgaaatg tgcagcagat cgtctgaaag tttttgacaa gatcatggcg aaagcgaaag
     6361 agtacggaat tgctccggac aggattcaca ttgatccgct ggttgagatg ctttgtactt
     6421 ctgaggatgg tattgcaacg aatattgaga cgatcacaag tgtacgtgag cagtatccga
     6481 ccattcatat tacggcagca atcagtaata tttcctttaa tctgccggtt cgtaagctga
     6541 ttaattacgg gttcctggta ctggcaatga atgcaggact ggacagtgga attatggatc
     6601 cgacaaataa ggatatgctt ggactggttt atgcaacaga aacattactt ggtctggatg
     6661 attattgtat ggaatatata ggggcttaca gagaaggctt aattggtaca acaaagaaga
     6721 agtaggttga atctgcagaa actgtgtaaa tgaattaagt ggttggaatc tgaaattgag
     6781 aaataacagg aggatttgat tatgtctaag ataaatgaag ttgcggaatt agttgaaaaa
     6841 gggaaagcga aattagtagg accggctgta caggaagcca ttgatgaggg tgatgatccg
     6901 gttgcgattc tgaatgatgg tatgatcagt gcgatgtctg tagttggtga gaaattcaaa
     6961 aatggtgaga tctttgttcc ggagatgctg gttgcagctc gtgctatgaa gaaaggtgtt
     7021 gaggttctga aaccgcatct ggcatctggt tctacaggtg cacttggtaa ggttatcatt
     7081 gctacggttt ccggtgacct tcatgatatt ggtaagaatc tggttgccat gatgatcgag
     7141 agtgccggat ttgaggtgat cgatcttgga gtcgatgttc cggcggctaa gattattgag
     7201 tgctataaag agaatccgga tgttaagatc attgctcttt ctgcgctgct gactactact
     7261 atgccggctc tgaaggagac tgttgaggcg ttgaatgcgg cggatttcag aggtaatatt
     7321 aaggttatgg ttggcggagc gccgattaca gaagcttttg ctgaggaaat tggtgctgac
     7381 ggatattctg atgatgcggc tagtgcggcg tcactggcta agaagttggc ggcctgatct
     7441 gattgctagt gcgggtttag tataacatga aaatattatg aggggactgc tctggggagt
     7501 ggtcctttcg tgtattttgg gatttttttg gagtgtgtta taatgggaaa aaggggacgc
     7561 tcagaaaaat attttatttc atgtgcggtt tcggggtcta agatattcta agttctcaga
     7621 aatctgctaa aggcatgagc agccttctca aactcgctcc gttgcaaact tcgctcagac
     7681 agtgatacag tctgctcata acgcagattt ctgagagcta agaatatcta agacccctgc
     7741 aaatgcacat gaaataaaat atttttctga gctggctgtt ggttgttgtg atgtttggat
     7801 tggatatgtg ggaggttttt atggcttggg ttaagtttgt gcgtgaaggg attgagatag
     7861 aagtgaatgc cgggatgtct gtgttggaag ctgagattcg ggcggatttg aggccggatg
     7921 cgccttgtgg cgggctgggg aaatgtggaa agtgtctggt taagattaat ggggaagtgg
     7981 ttaaagcgtg tcaggtgcgg atttgtgaag gtgagacttg tgtagtggag acattggaca
     8041 gggctggcaa tgaaaagatt ttgacggatg gatttaacag agaagttgtg tttgaaccgg
     8101 gactacggat ggctcaggtt gagttggaga aggcgaaaac aggggagaag cggtcggact
     8161 ggcagaggct gctggatacg ctggcggaga cagatggtga ggttgagccg gggcagatgg
     8221 aggtggatct gaagctggcg ggtgaattgt atgggatgcg cagggattct gatgaatggt
     8281 atgtgattta cagtagacgg cggattctgg aaatgaggaa ggaagctggt cggaggtgtc
     8341 tggcagcgtt tgatattggg acgactacca ttgcggggta tttgctggat ggtgcggatg
     8401 ggaggactct ggcagttgag agcaggatga atccgcaggc gcagtatggt gcggatgtga
     8461 tcatgcgggc gaattatgcg ctggagcatg gaacagaagc gttaagtatg tgtgttcgga
     8521 aagcggttaa tgagatgttg ggaagtctgg cagaggatgc agggataagg agagaggatg
     8581 tttttcaggt gtgtgttgtg ggaaatacct gtatgcatca tctgtttctg gggatttctc
     8641 cggcttcgct ggtacatgcg ccctatactc cggctgtgag tgagaggctg gtgctgaatg
     8701 cgggggatta tggtctggct gtgcaggagc gtgcagagtt gatcatgctt tcggatattg
     8761 caggatatgt gggagcggat acctgtggat gtctgctggc tatcaggcag gatcagcagg
     8821 aagagatttc tctgatgatc gatattggaa cgaatggtga gatggttctg ggaaacaggg
     8881 agcggatggt gacctgttct actgcggccg ggcctgcttt tgagggtgcg aagattgagt
     8941 gtggaatgcg tggcgcggcc ggggctgtgg atcatgtgaa gtatgaggag gggaagtgga
     9001 actatacaac tgttgggaat aagccggcgg ttggtttgtg tggttcggga ttgatcgatc
     9061 tggtggcggg tcttctggat gctggaatgc tggatgagaa tggtgtgctg agaagcggac
     9121 aggagaagca aggtgtgttt attctggtac cgccagagcg gggcggtaat gagagaggag
     9181 tttatctgac gcagaaggat ctcggagagg ttcagcttgc gaaagctgcg attgcagcgg
     9241 gaattcagat gcttatggag cggcttggga ttacagaaga tgatatctgt tcggtatata
     9301 ttgcaggtgc ttttggcaac tatatggatc cggtcagtgc cgggaagatt ggcttgcttc
     9361 cggcgacgct tgtgaagaaa gtgaaaccag tgggaaacgc tgctggtgag ggtgcgaaga
     9421 tcgcacttgt gaatgagaag gaaatgctgg agatggatga gctggtgaga aagattgaat
     9481 tcgttgagct ggctgcttcg gctgattttc aggatcattt tattgatgaa ctgggatttg
     9541 agacaggtga atgattttat ctgcctgatg aggttgctat agattataca agagagtaag
     9601 gggttagatt atggatataa aaaaaataga gcaaatggga atggagcagg ggttttcgca
     9661 tgtggtgttg ctggattgtg atacgattga gctgaagccg gaggttcgac agatgtgtgc
     9721 agcagatacc tgtcataagt atgataagtg ctggagctgt cctccgggat gtggctctct
     9781 tgaggaatgt gaagcgaaag tccggcagta taagtatggg attattgttc agacagttgg
     9841 tgaattggag gatgtttttg atggcgaggg aatgatggaa acggaagcca gacataagga
     9901 gtattttgtg gaatttgaga agaaacttcg ggagatttat ccggatatgc tggcgattgg
     9961 tgcgggatgc tgtacgaaat gtaaggtctg tacttatccg gatgctccat gcaggtttcc
    10021 gaaacaggct ttctcatcga tggaggctta cgggatgctg gtaacacagg tctgccaggc
    10081 caatgagcta cagtattatt atggagattg tacgattgct tatacgagct gttaccttct
    10141 ggaataacat tttagccgaa tcaggtaagt ccactgcgga gcagttattt ggtgtagcga
    10201 agcggattac gaatatctgc ttgatttaaa tcaagtaaat tctctggtta ctgttcactg
    10261 gtaatatccc gcgaggtgtt ttttgtgagc gaaggtcaca gaaaacccga gatataccgc
    10321 gcgaatttat gccattagca taaattaagt ttgcgaagca aacgatactc cttgggcatt
    10381 gcacaaggag gttcatattt cctgcatcgc gaagcgtgtt actgagaacg gagtgaacag
    10441 taacattctc tgtggaggtt gcggaagggg aagtcagttt tcgttgtaaa taacagtgag
    10501 ctatgataaa atacagatat tgaaagcgca gagcgtgttt gaaaaaataa aaaatccttc
    10561 gccgcagggc gggggatttt tggcagaagg tattaagggt ggcttgagga tatgacagta
    10621 ctggaacagt taagaaaaac aattgaagac ggacatccgg gggagacaga gaagctggtg
    10681 agggaggcat tgaagcagca tattcctgca gggcggattg tggaagaggc catgactccg
    10741 gctatgcgaa cagtggggga gaattacaaa tccaatggtg cggatattat taaaattctg
    10801 gcagcagccc gaagtgtgcg caagggtttt gaattgttgg aggaacagga ctcgcagttt
    10861 ggcaggagga atatcggaac tgtaatcctt ggaacggtgg aaggtgatct gcatgatgta
    10921 ggtaagaatc tggttgcgat catgtttcgt agtgccggat ttaaggtgat cgatctggga
    10981 gtggatattt ctgagaagca gtttctgcgt gcagtgaaac agaatccgga tgtgtcgatc
    11041 gtgtgtatct cttcacttct gagtacttca atcccggaga tggagcaggt ggtgaagtct
    11101 ctgaaacgaa gctgcagtaa gcataaattt aagattatgg ttggtggagg tgcagtaacg
    11161 gagcagctgg cgaagaatat gggagcagat gcctatacag agaactgtat tgaggctgtg
    11221 gaagttgcca aaacatttat tgtatgaaga tatatcttcc ttgtctttcg cactctgcag
    11281 aactgtctta aatgcattct ttacgaggat tgctatagat tgtttgcagt aatttatgga
    11341 agcactatag cattgactga caaaaatcgt gtaacaggag gacaggagac ttgcggctgt
    11401 catcagttta tttatatgaa aaaatacaac agcagtatga aattcaggag aagggaaatt
    11461 tatctggttc agacggatat ctgcgccctt ttttacgttg tggaataagt aaagaaggaa
    11521 aaaagtcaga agatggattt cagacaggac atgtatatgt aattcagggt tgtgatgcaa
    11581 atgaatgtga gccggtaaat tcgaaggcgg aaaatatttt ctggattttc tgtaaacgaa
    11641 aggatactgt cagagcagaa gagaatggag agactgtcgg gaaaggagct tatattcagg
    11701 tagcacttga tagtctggaa gaaatagcag aatttatgaa tgatgtgcag gagatttttg
    11761 atgcggctga tgaatgggaa cggaagattc atgatctgat gttagaacat gccggaatgg
    11821 acaggctttt gcaggtgaca tcggaatttc tgcagaatcc gatgactgta acaggactgg
    11881 attttacctt tgtggcggaa gcgggaagcg agtatctgcc gccgagggcg cggctgtata
    11941 cagatgacgg gttgaatatg gaatatgtaa atgcacttct gcagaatgag acttatcgag
    12001 atatggcaga tacgcatgaa tatgtgatgt ttccggctta catcagtgga tgtcgttcga
    12061 tgaacagaaa tctgtttgtg gatgggaaag caactcacag gctggttttg actgagtgca
    12121 gatcagagat tacactgaga gttatctgtg tactggatat tctggtggag aaactggagt
    12181 atttgcttgc gcatgaagcg gaggaagaag atccggacag ggatatggag cagatttttg
    12241 tcaggatttt gtcggacaga actgcggatt atatgcaggt gagtagagaa ctgagtgaac
    12301 tgggatggag tggaaatcat gaatatatgt gcctgatcct gcagattacc tatctgaatc
    12361 agcagaatct ttccacaaag gcaatctgcc gatatattaa gaagaaattt gaggattctg
    12421 tgagttttct ttatcaggat gaaattgtgg cattttttga ccttaccaga cttggaaaaa
    12481 gccaggagga agtagccgga aaactggttt attttatcag ggatacttat ctgaaagccg
    12541 gatacagcag ggtgatgaca ggacatatga atctgagacg gcagtatgtg caggcgaaga
    12601 cggctttgga tgtaggaagc cgtaagaagc cttatctgtg gattcattat ttcggtcagg
    12661 tggcattgac ttatattctg gaacaggcaa caagaagact gccgggaaca atgatctgtc
    12721 atgaagggct gctggaattg aagaagcatg atgaggagaa tcagactcag tacatggaga
    12781 cattgagagt gtatctggag cagcatctca gtgcaaccca ggcggcaagg gaattgttta
    12841 tccaccgcag tacgtttctt tacaggctgg acaggattaa ggaaatcctg cagtcggagc
    12901 tggatgatcc agaggagatt ttttatctgg aattgtcatt caggctgctg gagcaggagc
    12961 aagagaaaga atagtacggg aacagaatag tgcagatatc tggaaaggtc aattatttgt
    13021 tgagggagat gttttgataa aaaaattcag gtacgcctct gggcgtacct gaataaatgt
    13081 taaaagagat attatgttat ttagcttctt cttcggcagc agcctcttct tcagcttcca
    13141 gagccgggtc tacgtgaacg gcggctacgg atacaactgc agcttcaagg tctgtcttta
    13201 agtctacatc tttgtcctgg gcgatggcga ggtctttgac tttgattacg tcgccgactt
    13261 tcatgtcacc gacatctacc ataattttgt ctaccagtgc tgatgggaga gctctgaagg
    13321 agatttcttc cagatgctgc tgtaatacac cggcttccag tttgtcgtgg tttacaagta
    13381 cgatctctgc tacggaatgt actttctcgt tgcttaccag tgcctggaaa tctatttcat
    13441 ccacctgtcc ttttaacgga ttgaactgaa tctctttgat cagtacgtca tatgactgac
    13501 catctacatc cagcataatc tggcttcctt tgttatttgt cttgagaagt ttctcgacag
    13561 cagatttctc gattttaaca ggaagagaac cttccatctc tctgccaaat acgttacctg
    13621 taacgtagcc ttctcttctt aatcttttgg cctttgtgtc cattgttctt ttttcagctt
    13681 ttaaagtatt catttgactt ttcctccatt ttacagctga ggtgacacgc ttgacactat
    13741 cttcaattta tgagcatccg aaatgctcca ggtggtgtta agtattttta agtattcagt
    13801 tgtttgttct ttatctttgt tacagctgta ttataacacg ctaaaaatga aatgcaagag
    13861 gaaaatgtga agagtttgtg aaatttaagt aaacacaaac aaagccctcc ggaagtgacc
    13921 gcttccgggg ggggctttgc atttaatcta attaatcatt aattacagag tatcatttct
    13981 tctgatataa ccaggctgtt cagtcgggga aacgatttct tttttatgaa cgagtttcgc
    14041 ccatttgtcg actacttcat tctctacgtg aacaccctca cctacagtag cttcagcgag
    14101 aacaacactg tttcttacaa cagcaccttt tttaatgatg catccacgac cgattacaga
    14161 attctcaaca gtaccttcaa tctggcagcc gttggagatt acggaattct tcacatctgc
    14221 agtattgaaa tactgagtcg ggcaggaatc attggtacgt gtatagattg gccagttttc
    14281 atggaaaagt tcctgagctg acttgtagtt aaggagagcc atgtttgcag catagtaact
    14341 tgggaagtct gtaatgcttg caaagaatcc cttgtgtgca acgccgcgga tatccagagt
    14401 ttcacattta tcattcagga tgtctgtcag tgtgtacatg gaagaaagat tcttggcttc
    14461 tttgatcagg ctgatgaaaa gctctgtttt cataacacag gtatccatat aaacatactg
    14521 attcttctta ttaccatgat ttggttcgat ggattccaca cctttctggc ggttcagccc
    14581 cagaacatta caggtaagat aagcctcttt tgcattatca acagcgtgat aaaggagagt
    14641 aacatctgca ccggagtcaa tgtgggtatg taaaaactgg tcaaaatcaa ttgaataaac
    14701 catatagctc ggggcaatga caacatacgg atgatacatt ctgctgagtg attccatgtt
    14761 atccagatag caggagatat cggtgttata gatatcattg tcattattgt gctctgagaa
    14821 gagaagctga agtttgccgc tcttggagtt gatgttgtaa tgacgtcctg ttccaacgtg
    14881 ttctacaaca gaacggggtt tattattgat atagacctga atacggtcaa tgtcgctatt
    14941 ggtcatattg gagataggga agtcaatgac tctgtatctg ccaaggaagg aaaacgcacc
    15001 gatcgggcga taatcctgca taccatctac ataaatattt ctcccggaag agcttacgat
    15061 tccaaatgcc ttatacatat tattctgccc cctttacgcg tttcgctaca agctcaatgt
    15121 gctcactgtc tgcggcaccg acaacggcac ctttaccgat gcggataccg tcagctacaa
    15181 gtgcacgctg aacaacggca ccttcttcta ccacaacacc cggcatcagt acactgtcga
    15241 taattctggc acctgcacca atcttaacac ctgtaaagag aacggaatgc ttaacttcgc
    15301 cctgaacaac acagccctgt gtgatatatg catctttaac atctgcttct gcgctgatgt
    15361 actgtggaag tgcggcaaca tcttctgtat aaatcttcca tgacggatcg cccagatcaa
    15421 gctcattttt ggaacttaac agatccatgt ttgcttccca gagagagtcg atggtaccaa
    15481 catctttcca gtatccttca aatttatatg cataaagagt cctgttatcg ttaagcatag
    15541 tagggatgat atctttaccg aagtcatggc tggaatcagg attcttgata tcagccatca
    15601 gcatcttgcg gaggagtttc caggtgaaga tatagatacc catggatgca aggttgctct
    15661 tcggatgctc aggtttttcc tcaaactcta cgatacgtcc ggattcatca gtgttcatga
    15721 taccgaaacg gcttgcctct ttcatcggaa ctccgataac tgcgatggtt gcatctgcct
    15781 ttgcttcttt gtgggctgca agcatcttgt catagttcat tttgtaaata tggtcaccgg
    15841 aaagaacgag aacgtattca ggatcaaatt tatcaatgaa atcaatattc tgggagattg
    15901 catctgctgt tccgcggtat acattcagat tctcatctgc tttctcacgt ggaggaagaa
    15961 cgtaaacacc gctgttttta gcatcaagac cccagcgtcc gccggctgct acatagctgt
    16021 tcagctggat agattcatac tgtgtaagta cacctacaac atcaacgcca ctgttggcac
    16081 agttacttaa aggaaagtct acgatacgat atttaccgcc gtatgaaacg gccggctttg
    16141 ccactttatt ggtaagatca tgcagacggc tgccacgacc gccggccagg atcatggcca
    16201 acatactatt ttgtttcata atagtgaacc ctcgctttca tttgttatgt aatattatac
    16261 attttttcct gacacttttc cataggtttc tgaaaaaaat atgtaaaatt tttgctacaa
    16321 actaagtaaa atcgacaaaa aattatacat tatatacaaa tatcgagaca aatcgcatga
    16381 caggggatta aagacatttg taaattatgc ttataatgta ccaaaattca ttccagattg
    16441 cctgcggcaa taggattttg gcctgtatgt ctcgggattt tggcatattc atgccaaaac
    16501 actttgctga ataatggtgt gcaaacagta acatttgttc acaaatttaa gtgaaacggg
    16561 tggacgtata atagttctta cgctataata ggtaggaatt tatagaagaa acgcgtaact
    16621 gtgaaacact gaacagttac agagaaacag aggttatcac gtgagtaaaa gagaagatta
    16681 taaacgattt atcgtatttt gtctggcgag tctggtggtt ctggcacagg cggcggtctt
    16741 tgcatgggtg tggtattccg tttacagagg acagatcgac gagcctttct ggcgcaaagg
    16801 taactgggta ctgatcgcaa tctacggtct gatgtttgcc ctgtttgcca aattgtacgg
    16861 aggacttaag gttggatatc ttaagaggat tgatgtattt tattcgctga cactggcact
    16921 tttgtgtacc aatgtggtgg aatatctgga gatcaccctg atcaacagat ggttcctgag
    16981 cgtatggcct atgatcgaga tgacaggaat acagctggtg ctgatcatta tctggatatt
    17041 tggttcaagg tatatctatt ccggacttta ccgcgcacgc cggcttctgg tcatttacgg
    17101 tgaccgggat ccgggggacg acctgatcca taagatgaat tcccgaaagg acaaatatga
    17161 catcagcggc aaggtacaca tcagtgtggg agaagaaaaa atccatcaga tgatgcagga
    17221 ttatgacggc gtgatcatct gggatctgca ttcaacagag agaaaccgtt atctgaaata
    17281 ctgttttgca cattctgtcc gatgctatgt gagtccgaag atttcggaca tcattcttat
    17341 gggaagtgaa cgcatccatc tttttgacac accgcttctg gtatccagaa atatgggact
    17401 ggcagtagac cagcgtgctg caaagagagt tatggatatt ctgatctcag ggatcggcat
    17461 tataatcact tcaccgatca tgctgatcat agcgattgcg gtaaaagcat acgacagagg
    17521 tccggtgttt tattttcagg accgtctgac attgggcgga aagcccttta agatctgtaa
    17581 attccgcagt atgtgcgtag attcggagaa aaacggtgca agactggcat ccaagcatga
    17641 ttcccgtatc acaccggtag gtcatgtgct gagaaatctt catctggatg agcttcctca
    17701 gcttttcaat gtatttaagg gagatatgtc tctggtaggt ccgcgtccgg aacgtgaaag
    17761 tatcatgctt gagtatgaga aagagcttcc ggaattctat tatcgtctga aagttaaagc
    17821 aggtcttacc ggatatgcgc aggtttacgg taagtataat acaactcctt atgacaaatt
    17881 aaagctggat ctgttttata ttgagaatta ttctttcctg ctggatatca agctgatctt
    17941 tatgacagtc aaaatctttt tccagaaaga agtatctgag ggagtagatg acagacaggt
    18001 aaatgcgttg aaggattccg ggaaaaacgc cggagtgtca gagaataaaa cggaggaatg
    18061 acaatgaata aaccgctggt atctgtgatc atgccggttt acaacggaga aaaatatatt
    18121 agaaaggctg tggaatccgt ctatgaacag ggagtttcgc tggaacttct ggtgatcgat
    18181 gacggttcta cagaccatac agaggaagtc ctgtctgcct atgagggcag agaggacttt
    18241 cggtatatta agaatgaaca gaatatggga gctgcaggct ccagaaacag aggagtcggg
    18301 ctggcacagg gaacttacat tgcttttctg gatgcagatg actggtggga gtcaggcaag
    18361 ctgaaagaac agctgaaacg tctggaagag acaggatacg tgctgtgttc tacagggcgg
    18421 gaactgatga aagcggacgg aagcagtaca ggacggacaa tccctgtaaa agagaagatc
    18481 acctacagag aacttctgaa acataacagc atcaactgtt cttcggtaat actcagaagg
    18541 gatgtggcgc gtgaatttcc catggagcat gacgacagcc atgaggatta tatcacctgg
    18601 ctgaaaatcc tgcgaaaata cggctgtgct gccggaatca acaaacccta tctgaaatat
    18661 cgtctcagcg aaggcggcaa atccagaaac aaactgaaat cagcagccat gacttataat
    18721 gtctacagat acgccggtta cggtaggatc agaagctgta tttttttctg ttcttatgct
    18781 gtacatggaa tatggaaata ttgctactgt tcacttcgtt cacagtaacg tagccaaaat
    18841 tcattccaga ttgcctgcgg caatggaatt ttggcttgta tgtctcggga ttttggcata
    18901 ttcatgccaa aacacct
//
LOCUS       DBMDXG010000005        18715 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_911_length_18715_cov_2.480015, whole genome shotgun sequence.
ACCESSION   DBMDXG010000005 DBMDXG010000000
VERSION     DBMDXG010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 18715)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18715)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18715
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_18715_cov_2.480015"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="ACZPD3_00430"
     CDS             complement(<1..824)
                     /locus_tag="ACZPD3_00430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR1414519.1"
                     /translation="MKKKIYLAMLAVCFALTASACGDDGAVITDGTKTEGAADGKKAE
                     TGTTRLVSVENVEKYITIGEYKGLTLDNTVDAITDDDVQAQIDEDLKDKAEPVSDAAK
                     EGDLVTVNYTGTKDGQTFDGGTANNYDFVIGDGQMFEEFENGVIGMKKGDTKEIKIDF
                     PSDYADETLAGEEVIYKVTVQNVRREGELTDEWVAKNTDYTTVDDYRESIRSELEKNA
                     KESAQEVLKNTAWSTVLENSEVKEYPQEDVDKAVSEFKKSMEVYAKQADMTLEEFT"
     gene            complement(843..2297)
                     /locus_tag="ACZPD3_00435"
     CDS             complement(843..2297)
                     /locus_tag="ACZPD3_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1414520.1"
                     /translation="MRKRIACVTAAILSISLGVSGCGLIPFEQDIPFLSSRAENAETE
                     KKTEQQTIDEQDADTEKSTEDTQQDDGLNEGEQTDNEDTEEADEKQENPMEQAALMAV
                     QYDYDGAIELLKSQPDYESNTDMQSAVSDYENTKATCTEYPLEQITHVFFHTLIKDTA
                     RAFDGDSDSNGYNQYMTTIDEFNKIIQSMYDKGYVMVSPHDMAVINEDGTMSRGSIML
                     PPGKIPFVLSQDDVSYYHYMDGDGFASKLVVDSNGEVKNEYIEDDGSVSTGDYDMVPL
                     IDTFVKEHPDFSYHGRKGILAMTGYDGVLGYRTDIAYKTGKKLQDDQKKFLEDHPDFN
                     YKQEVKNAKKVAKAMKAEGWEFASHTWGHKDVAATSLDDLKRDDKKWKKYVAPILGET
                     DMIIFAFGADIGSWEGYSADNEKYEFYKSQGYRYFCNVDSSQYFVQITDDYFRQGRRN
                     LDGYRMYYNPEMLSDLFDVSEVWDSSRPTPVPGM"
     assembly_gap    2503..2512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2513..4015)
                     /locus_tag="ACZPD3_00440"
     CDS             complement(<2513..4015)
                     /locus_tag="ACZPD3_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1414521.1"
                     /translation="MSELKPDSEKKEERLSDKEKVNTEKTNAEELNAEEPNAEKPNTE
                     EINEEPPAASEDEDFFFDDNKAYEARRAARLERRNKLRRRKKRLRIAGCIVVVAAAAI
                     GIGIGFYGEELQNFVSEQQVKIAQMVKSADRKTEEEKTAQQTNAEAEAENAVTPEVQD
                     TDGLSEEDKTLYRQAKYAAKQYDYDKAISMLQNSETYQTSEKFQHAVKVYQKKKESCV
                     SWPLDQVTHVFYHTLIKDTGKAFDGDYKSGDYDQVMTTIDEFNQITQSMYDKGYVMVS
                     IYDMATADENGNMNAGEILLPPGKVPFVLSQDDVCYYHYMDGDGFATKLIVDEEGKIR
                     NEYVEDDGSISVGDYDMVPLIDRFVEEHPDFSYRGAKGIVALTGYNGILGYRTDSSYE
                     TRPDDLDADKVKWLDEHPDFNLNTERENAARVAQAMKDEGWLFASHTWGHQNVSQISL
                     ERLQADTQKFKENVDPLIGGTDIIIFAFGADLTSVEDYSGEKFEYLKSQGY"
     gene            4365..4820
                     /locus_tag="ACZPD3_00445"
     CDS             4365..4820
                     /locus_tag="ACZPD3_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414522.1"
                     /translation="MSTVTIVLLVILVILIAALIGLYFFGKKAQKKQEEQQAQIEATK
                     QTVSMLVIDKKRMPLKESGLPQMVIEQTPKLMRRSKLPIVKAKIGPKIAIMIADEKVF
                     DLIPLKKEIKAEVSGLYIVGVKGIRGSLQTPAPKKKGFFAKFKKDKTAR"
     gene            complement(4944..5390)
                     /locus_tag="ACZPD3_00450"
     CDS             complement(4944..5390)
                     /locus_tag="ACZPD3_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiB family protein"
                     /protein_id="HIR1414523.1"
                     /translation="MDKEVLIHVRGLQTMDADGEQEPLEIVVPGQYYFRNGSHYLRYE
                     EVMEDFAEPTVNYIKISRKGMEVRKKGVVNVHMVFEQGKKNMTYYTTPYGTIEMGIAA
                     TNLNLQESDGGLDMKVDYALDMNQEHVADCYLAIKAQPKDCKNFTI"
     gene            complement(5472..6293)
                     /gene="murI"
                     /locus_tag="ACZPD3_00455"
     CDS             complement(5472..6293)
                     /gene="murI"
                     /locus_tag="ACZPD3_00455"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR1414524.1"
                     /translation="MKIDREAPIGVFDSGVGGLTVAREIMRNLPSEKIVYFGDTARVP
                     YGSKSKETIIRYSRQIIRFLQQQQVKAIVVACNTASAFALDAVRDEFDIPIIGVIEPG
                     AKVAAAQTRNKRVGIIGTVGTVGSGIHAEYLKHLDPEITVFGKACPLFVPLVEEGWLH
                     DPVTDEVAARYLKELQDKQVDTLILGCTHYPLLRSTIRKIMGDGVCLVNPAYETALEL
                     GRLLEEKGLAGEGTEKNEFPYRFYVSDLADEFKAFANSILPYDVEMTKKIDIEKY"
     gene            complement(6336..6611)
                     /locus_tag="ACZPD3_00460"
     CDS             complement(6336..6611)
                     /locus_tag="ACZPD3_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414525.1"
                     /translation="MMNKKSKNKKLRRKLSFVLFVAAVGVLMMRAMPGMSGKIAKIAE
                     RRQEQTAVTAWWGTLYPKFCFSQFPEENKGQKDDIKISFWLAQALDW"
     gene            complement(6742..8427)
                     /locus_tag="ACZPD3_00465"
     CDS             complement(6742..8427)
                     /locus_tag="ACZPD3_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HIR1414526.1"
                     /translation="MRFAENNRISHRQLYRQMILALLAPFMLCVFGKGGMNGISAVAG
                     MIFALILLGFYVIWLIRLTPSFEDPVKSAGAFAGRLIGIFFLIYVLMAGGYLLALLRR
                     LVPVKLITGVSGRWIAFWAVLVCSVGTCKGVQRRGRMAEVSGGLLLGGIMIMMILCVP
                     QAKTEYLVGGIRWEELTVRNVRQSFYGTLCAFSPVALLPFLLGNVEKYGSAGKTVAGG
                     ILTLGGILIGMELLLPAVLGYDRVAAESYPVLPLLAGADLPGNVLARFDILWMGFLLY
                     SLLFAIGSLLYYGNQIIGKSHPGRGRFWLPALVFLISLLEEEGKGILDYFGWCLAHIF
                     VPGILICQFYMFIRGKGHRRKQRKRAVSVVTGILSVSLFMSGCGAAVEPEKRMYPMAL
                     GVDASEEGICLTYGMPDLSESTGQGKEEEDGGSRVLQISGADFTRIEKMYDQSQEKLL
                     DMGHLQVLVMGRTLVEDGRWRMVLDYLKQEIFVGEDLYVFEAEDAGEILNWHGEDNSS
                     AGEYITGLIRNRMSGGNITAVTLRELFYEKYKEDKILRLPIVKIRNGSLEVEV"
     gene            complement(8424..9917)
                     /locus_tag="ACZPD3_00470"
     CDS             complement(8424..9917)
                     /locus_tag="ACZPD3_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR1414527.1"
                     /translation="MEESRKVSANLRENEKYLRSRLENCSDILIRPMRLGDKHKVDCL
                     MVYIEVAVSNMMLDDSALGKMINHFWEISPEDIQEFVRHNSLGIADVKKLENLDESID
                     AMLAGNAVFFIDGYDKAMKISSKGYPSTGVMEAESEKVLRGSREGFSDSVKSNSALVR
                     KRLRDTRLKVEEYKIGVRSHTLTQVLYMDDLVHEGLLEEVKERLEEFQIDGILDSGML
                     EQLTEDVWYSPFPQYQTTERPDRAVQEILKGKVVILCDNSPEALILPGNFSSFMESSE
                     DWYHRFEMASFLRILRYLAVIMATVLPGLYLAVIRFHTQILPSALILSFAEAREGVPF
                     SSVVELIFLELAFELIREAGVRVPGSLGNAIGIVGGLVIGQAAVEANLVSPIVVMIVA
                     LTALGSMTVPNEEFAAAFRLVKYGFLILGGYLGIYGIVLGVYLVIGHLAGLISFGIPY
                     LVPFIKKEQKGSRGEGVLRVPLRKRVLRPLYAREEQKIRLKRKESGS"
     gene            complement(10092..10811)
                     /locus_tag="ACZPD3_00475"
     CDS             complement(10092..10811)
                     /locus_tag="ACZPD3_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1414528.1"
                     /translation="MTSDFSVNMNEYLPLRDVVFNTLRQAILKGELKPGERLMEIALA
                     ERLGVSRTPIREAMRKLEQEGLVVMIPRRGAQVANITEKDLNDVLEVRIALENVAIEK
                     ACARMTEEEMRRLWLAAKEFEHTIAEGNLVKLAEADVAFHEVIYQASDNKRLIQVLNN
                     MREQIYRYRVEYLKEGETRDVLVKEHEELTKAIRERDVERAKQLSFQHIENQRMAIMR
                     SIEAEDAERERAEKEKSRGHR"
     gene            complement(10801..11679)
                     /gene="ispE"
                     /locus_tag="ACZPD3_00480"
     CDS             complement(10801..11679)
                     /gene="ispE"
                     /locus_tag="ACZPD3_00480"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1414529.1"
                     /translation="MRLRALAKINLGLDILRKREDGYHEVRMIMQTIQMYDVLEMKRV
                     RKPGISLSVNYSYIPNDERNLVYKAAKLLMDEFQVKGGVDIHLEKFIPVAAGMAGGSS
                     DAAAALVGINRLFKLGLSQKELMDRAVNIGADVPYCVMRGTALAEGIGEKLTRITQVP
                     DCFVLIGKPGINVSTKAAYESLQLDKISSHPDIDGMIGDIERGDLLAMTQKMGNVFEP
                     GIIEKYPVIGEIKALMESHGALKAMMSGSGPTVFGIFDDREKMEAAAEVLRESRLAKT
                     VFATEVTKAGGNTNDK"
     gene            complement(11839..13389)
                     /locus_tag="ACZPD3_00485"
     CDS             complement(11839..13389)
                     /locus_tag="ACZPD3_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR1414530.1"
                     /translation="MELKKESIQMLRIKNKAAAQATFDEDYNVSDVKPDIGRLVQSKA
                     DVSMEEVRLSEGRALLKGTLNADLLYVGEKEGRIYSLSAKLPLDEMINLEGIEGGDKL
                     CLKWEIEDLSVHMIHSRKLNIKAIVTFYAVVDELAVVELPVSAEDQEVSVKTEKVRLM
                     SLRVHKKDTLRIKDDITLASNRPNVENLLWYMAEPRNLDLRPGENKLRVKGELAVFLL
                     YTGYEEENPPQWLEYTMPFSNEMECSGCMEDLIPHIEVSLLHQGIEVKPDPDGEERIL
                     QVDVVLELNMKMYREEEHELLLDAYSPHKECVLHRKKEMLESLLVRNFSRCRLTDRIE
                     VKESQGKVLQLCHSSGKVKVDKTRITDKGIVAEGIVALKILYIIGNDEMPFYSMDAML
                     PFTHLIEAEEIGKDCTYLLQADLEQLSTAMADGDEIEVKAAVGLNVLVFRQWEEQLIE
                     SVEEQPLDRKKLESMPGITVYIVKAGDTLWDIAKKFYTTVDEISGVNSLTEKEVKPGQ
                     SLILVRQG"
     gene            complement(13412..13834)
                     /locus_tag="ACZPD3_00490"
     CDS             complement(13412..13834)
                     /locus_tag="ACZPD3_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR1414531.1"
                     /translation="MGKRTGGLLCVLLLALLPAGCQFIRIEEGERTPLEYTIVKQEEI
                     PEEISELMEQKKKKVFQMTYQVGDIRYLMKGYGEQLTGGYSIQVEEVSESENAVFCKT
                     RLIGPEKADAGSEPSYPCIVLKIRETEKPIEFLGFIIK"
     gene            14088..14834
                     /locus_tag="ACZPD3_00495"
     CDS             14088..14834
                     /locus_tag="ACZPD3_00495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1414532.1"
                     /translation="MIRFILVCIVVIGYLILSIPILLVEWIIGRFSPEKKDISSLRII
                     QAVFRFILKITGAKITVIGEENVPKDTPVLYIGNHRSYFDILLTYSRCPIRTGYIAKK
                     EMEKIPLLSTWMRYLHCLFLDRKDIKQGLKTILTAVDKVKSGISICIFPEGTRNRNKD
                     ELDMLPFHEGSFKIATKANCPIIPIAISNSANIFEAHFPKISPAKVVVEYGKPIYPDE
                     LSKEDKRHVGEYTQNVIREMLIKNKPLTEK"
     gene            complement(14900..16060)
                     /locus_tag="ACZPD3_00500"
     CDS             complement(14900..16060)
                     /locus_tag="ACZPD3_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019149.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1414533.1"
                     /translation="MLNICIVFAQLERETIQKRVSDAYYSRSQKGFRMGGKPPYGYRL
                     EEILMEGIHTKKLVEEPGEAAIVREIFDMYEQPDTSYGDITRYYAEKGVQFYGKELIR
                     SMLAQLLRNPVYVRADMDVYRFFRSHGTNIVSSPEQFDGIHGCYLYQGRDAQTDKLQN
                     LKGHMLVVAPHEGLVSSEQWLNCRIKLMRNKTIQANRKAVNTWLAGKVKCGNCGYALM
                     SIKIQSGKQYLRCTKRLNNKACPGCGKVYTEDVENYVYKEMVRKLREGQSPATYTKLN
                     ENPQVKQIYREIEEMEKEISLLVDSLAGAGETLTDYINQRVEEIDQMHQLKLEKLSVL
                     AENHATPEQMEKVASNISLWGEIDFEEKRFTVDKMIRSLKVFPGLVQIQWKF"
     gene            16044..16346
                     /locus_tag="ACZPD3_00505"
     CDS             16044..16346
                     /locus_tag="ACZPD3_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414534.1"
                     /translation="MQIFNIASIYKGVVNSDSFSVHDFDILTLYPLDFEEFLWANAEF
                     ALTREIRNHFSDFSSMGKKLHEKALSQFRLYLIIGGMPAAIMEYKKEKKLLLVPDT"
     gene            16336..16740
                     /locus_tag="ACZPD3_00510"
     CDS             16336..16740
                     /locus_tag="ACZPD3_00510"
                     /inference="COORDINATES: protein motif:HMM:NF025019.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HIR1414535.1"
                     /translation="MTPSKSSLHLYPVDTGLCSCILKIPAFQLLSGEETTAGTACIET
                     FLAQHFIRNGYALSYWSSGNQAEVPFLLSKNSHFIAIDYRTTPHQKCRNLMQLNKSSL
                     SPAPTQMYLISAEDFRQKEAYQIVPLYAVFCI"
     gene            complement(16900..17910)
                     /locus_tag="ACZPD3_00515"
     CDS             complement(16900..17910)
                     /locus_tag="ACZPD3_00515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpnC/YadD family protein"
                     /protein_id="HIR1414536.1"
                     /translation="MLDTAEKNKIKPDTILKNFWRDNRHFADLFNAVFFNGEQVLKPQ
                     DLTEADTDVSSMLKFNGHAETVQKILDVVKKTAYGVDFVLWGLENQAKIHYAMPLRHM
                     VGDAFSYMKEYDEIAAVNKKNGDFHSADEFLSGFKKTDRLHPVISLCVYYGESEWDGP
                     FSLKDMLEIPEKIESLVSDYRMNLVQVRSSGELCFSDPDVNMVFDVSRLIYARDYTKI
                     NEVYRDHNIPADLGLVIGAITESQKLIDHALESEQKGGQINMCNALEELRQEGVEEGR
                     QEGRQEGRQEGRWEGILEGIRATVRTCRNFNISETDTVRNIMNEFSLSQEEAVNYVKK
                     YW"
     gene            18205..>18715
                     /locus_tag="ACZPD3_00520"
     CDS             18205..>18715
                     /locus_tag="ACZPD3_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414537.1"
                     /translation="MTKIKNTKKGVAKKTLSMSLVVAMLATSNVPVWAAEFSDDFDAA
                     VATEAPVTEFSDNTIETPAIEEETTEAPMAQAVVESDDLSVDVSVSESAEFTGIVSVS
                     GTINASDLDKWGIDKQTIANYDVTIKDGVATVINDTIKVPVSEYTVTKNSDDTYTVAA
                     KSTSKNYTGS"
BASE COUNT         4939 a         5071 c         3069 g         5626 t
ORIGIN      
        1 tctgtaaact cttccagagt catgtcggcc tgttttgcat aaacttccat actcttctta
       61 aattcagaaa cagccttatc cacatcttcc tgtgggtatt cttttacttc agagttctca
      121 agcactgtac tccatgcagt attcttaaga acctcctgcg cagattcctt tgcgtttttc
      181 tcaagttcac tgcgaatact ctcacgataa tcatctactg ttgtataatc agtgttcttt
      241 gcaacccatt catctgtaag ttctccttca cgccgcacgt tctgcaccgt aaccttatat
      301 ataacttcct cacctgccag agtttcgtct gcatagtctg acggaaaatc aattttaatc
      361 tcctttgtgt cacccttttt catcccgatc acaccatttt caaattcctc aaacatctgt
      421 ccgtcgccga tcacaaagtc atagttattg gcagtaccac cgtcaaaagt ctgtccgtct
      481 ttagtgcctg tataatttac agtcaccaga tccccttctt ttgccgcatc agatacaggt
      541 tctgccttat ctttcagatc ctcatcgatc tgcgcctgta catcatcgtc agtgatcgca
      601 tccaccgtat tgtccagagt cagtccttta tattctccaa tcgtaatata tttctctaca
      661 ttctcaacag aaacaagacg cgtagttcct gtctctgctt tcttcccgtc agcagctcct
      721 tctgtctttg taccgtctgt gataacagca ccgtcatcac cacacgcaga tgctgtcagt
      781 gcaaaacaaa ctgcaagcat ggcaagatat atttttttct tcataataat caatactccc
      841 ttctacattc caggtaccgg agttggtctt gaagaatccc atacttccga tacatcaaaa
      901 agatcactga gcatttccgg attataatac attctgtatc catccagatt tcttcttccc
      961 tgtcgaaaat aatcatctgt aatctgtaca aaatactgac tggaatctac attacagaaa
     1021 tagcggtatc cctgactctt ataaaactca tatttctcat tgtctgccga atatccttcc
     1081 cagcttccga tatccgcacc aaatgcaaaa atgatcatat ctgtttctcc aagtatggga
     1141 gccacatatt tcttccattt tttatcatct cttttcagat catccagaga agtggcagcc
     1201 acatccttat gtccccatgt atggcttgca aattcccagc cttctgcctt catagcctta
     1261 gctacctttt ttgcattttt cacttcctgc ttatagttaa aatccggatg atcttccagg
     1321 aattttttct gatcatcctg cagttttttt ccggtcttat atgcaatatc cgtacgatat
     1381 cccagaacac cgtcataacc tgtcattgca aggattccct ttcttccatg ataggaaaaa
     1441 tccggatgtt cttttacaaa agtatcgatc agcggaacca tatcataatc ccctgtggaa
     1501 acacttccgt catcctcaat atattcgttc ttaacctcac cattggaatc cactacaagt
     1561 ttcgatgcga aaccatctcc atccatatag tgataataac ttacatcatc ctgagaaagc
     1621 acaaatggaa tttttcccgg tggcagcata atactgcctc tggacatagt tccgtcctca
     1681 ttgataaccg ccatgtcgtg cggactgacc atgacatatc ccttatcata catactctgt
     1741 atgattttgt taaactcatc aattgttgtc atatactggt tatatccatt actgtctgaa
     1801 tccccgtcaa atgctctggc agtgtctttg atcagtgtat ggaagaacac gtgcgtgatc
     1861 tgctccagcg gatattctgt acaggtggct ttcgtatttt cgtaatccga aactgcactc
     1921 tgcatatccg tattactttc gtaatccggc tgactcttaa gcagttctat ggcgccatca
     1981 taatcatact gtacagccat aagcgctgcc tgctccatag gattttcctg tttctcatct
     2041 gcctcttctg tatcttcgtt gtcagtctgc tctccctcat tcagaccatc atcctgctgt
     2101 gtatcttctg tacttttctc tgtatcagca tcctgttcat caattgtctg ctgttctgtt
     2161 tttttctcag tttccgcatt ttcagctctg gaactcagaa atggaatatc ctgttcaaac
     2221 ggaatgagcc cacatccgct tactcctaac gaaatactca aaatagctgc tgttacacat
     2281 gctatccttt ttctcatata ttgtcccctt tcagttatgc acagagatca tactcacccc
     2341 atagttggaa ccggcaccgg tctggaagaa tcaaacacag actgtgcatc aaacagatct
     2401 gagagaagtt ccggattata gtacattctg tatccgtcca gatttcttct tccctgccgg
     2461 aaataattac tgcggatctg cacaaaatac tggcttgaat ccnnnnnnnn nnatagccct
     2521 ggctcttaag atattcgaac ttctcccccg aataatcttc aacactggta agatctgctc
     2581 caaatgcaaa aataataata tctgttccgc ctatcagagg atcaacattc tctttaaact
     2641 tctgtgtatc tgcctgtaat ctctccagac tgatctgact tacattctga tgtccccatg
     2701 tgtggcttgc aaacagccag ccttcatctt tcatagcctg cgctactctg gctgcatttt
     2761 ctctctctgt attcagatta aaatccggat gctcatccag ccattttact ttatctgcat
     2821 ccagatcatc cggacgggtt tcataactgc tgtctgtgcg ataaccaaga attccattgt
     2881 aaccggtaag ggcaacaatt cctttagcac ctctgtaaga aaaatcagga tgttcctcta
     2941 caaaacgatc gatcagagga accatatcat aatcgcctac agaaatactg ccatcatctt
     3001 ccacatattc attgcggatt tttccttcct catctacgat aagtttggtt gcaaatccgt
     3061 ccccgtccat ataatgataa taacacacat catcctgcga aagcacaaac ggtactttac
     3121 ccggaggaag gagaatctcc cctgcattca tattcccatt ttcatccgct gtcgccatgt
     3181 cataaatgct gaccatgaca tatcccttat catacatgct ctgagtaatc tggttaaatt
     3241 catcaatcgt tgtcataacc tgatcgtaat ctcctgactt gtaatcccca tcaaaagcct
     3301 taccggtatc tttgatcaga gtatgataaa agacgtgtgt cacctggtct aacggccagg
     3361 aaacgcagga ttccttcttt ttctgataaa ccttaaccgc atgctgaaac ttctcacttg
     3421 tctgataagt ctcactgttt tgcagcatac ttatggcttt atcataatca tactgttttg
     3481 cagcatactt tgcctgcctg taaagagttt tgtcctcttc actcagtcca tctgtatcct
     3541 gaacttcagg tgtcaccgca ttctcagctt ctgcctctgc atttgtctgc tgagctgttt
     3601 tttcttcttc tgttttcctg tctgcagatt tcaccatctg cgcaatctta acctgctgct
     3661 cactgacaaa attctgcagt tcttccccgt aaaatcctat tccaatccca attgcagcgg
     3721 ccgccaccac cactatgcag cctgcgatac gcagtctttt ttttctgcgg cgaagtttat
     3781 tgcggcgttc cagtctggct gccctacggg cttcataagc tttattatca tcaaaaaaga
     3841 aatcttcgtc ctcgcttgct gccggaggtt cttcgtttat ctcttctgta tttggttttt
     3901 ctgcatttgg ttcttctgca tttagttctt ctgcatttgt cttttctgta tttacttttt
     3961 ccttatctga aagtcgctcc tctttcttct cagagtcggg ctttaattcg ctcatgcttt
     4021 tctccttttt atcatatccg ttttttactc acaaattatt ctgtcacaaa ataactccac
     4081 aattcccgtt acatatatta taaccacaca ttatgattcc atcctgtaaa aatgtcttta
     4141 actgttccgc atatttacca ttactgttcg ctccggcaaa tattgtcgca ttacagtttt
     4201 gctacaatta taacctttca taatgtaaat ttctaccgct ttccatccat taattctttt
     4261 tttgcaattg cgttttacct tatttaatga taaaataatt tttatgcaat ggactgtccg
     4321 taccaacagt ccctgtgaaa acacatacat tagggaggat gcaaatgagt acagtaacaa
     4381 tagtccttct ggtcattctt gttatcctga tcgctgcact gatcggttta tatttcttcg
     4441 gaaaaaaagc ccagaagaaa caggaagaac agcaggctca gattgaagca actaaacaaa
     4501 ccgtttccat gctggttatc gataaaaaga ggatgccact gaaagaatcc ggacttcctc
     4561 agatggtaat tgaacagaca cctaaactta tgcgccgttc caagcttcca atcgttaaag
     4621 ctaaaatcgg accaaagatt gcgatcatga tcgcagatga gaaggttttc gacctgattc
     4681 cgcttaagaa agaaatcaaa gccgaagtaa gcggtcttta tattgtaggc gtaaaaggaa
     4741 tccgcggttc ccttcagaca ccggctccca aaaagaaagg tttctttgca aaattcaaga
     4801 aagacaaaac tgccagataa atgcagtcag acatataaac taaaggtcag tgaatcgtgt
     4861 catccataca cacagatcac tgaccttttt ctgttttgat cagacagata tctgcaacga
     4921 agcagagaac ttaccagatc tgatcaaatt gtaaaattct tacagtcctt tggctgtgcc
     4981 ttaatcgcaa gataacagtc tgccacatgc tcctggttca tatcaagcgc ataatccact
     5041 ttcatatcaa gaccgccatc actctcctgc agattcagat tcgtcgccgc aatccccatt
     5101 tcaatggttc catagggagt ggtatagtaa gtcatattct tcttaccctg ttcaaaaacc
     5161 atatgaacat tgaccacacc tttcttgcga acctccattc ccttccggga aatttttatg
     5221 taattgacgg taggttctgc aaaatcctcc atgacttcct catagcgcag ataatgactt
     5281 ccgttccgga aataatactg tcccggtaca acaatctcca gaggttcctg ttcaccgtct
     5341 gcatccattg tctgaagtcc ccttacatga atcagcactt ctttatccat tcttctcata
     5401 ctcctgtttt tatactgttc aaaacctgtt tttatgtatc tatattataa ccattcagca
     5461 atctgctgtt tttaatactt ctcaatatct atttttttcg tcatctccac atcatatgga
     5521 agaatcgaat tggcaaatgc cttaaactca tctgccagat cactcacata gaagcgatac
     5581 ggaaattcat tcttctctgt accttcaccg gcaagtcctt tttcctccag aagccttccc
     5641 agctccagtg cggtttcata agccggattc accagacata ctccgtctcc catgatcttt
     5701 ctgatggtgg aacgaagcaa tggataatga gtacatccca atatcagtgt atccacctgc
     5761 ttatcctgaa gttccttcag atatctggca gcgacctcat cagtcaccgg atcatgaagc
     5821 cacccttctt ctaccagtgg cacaaacagc ggacatgctt tgccgaaaac agtgatctcc
     5881 ggatccagat gcttcagata ctctgcatgt atcccacttc ctacagttcc cacagtacca
     5941 atgattccta ccctcttatt tctggtctgg gcagccgcca ccttcgcacc cggttctatc
     6001 acgccaatga taggaatgtc aaactcatcc ctcactgcat ccagggcaaa agcgctggct
     6061 gtattacatg caaccacaat cgcctttacc tgctgctgct gaagaaaacg aatgatctgc
     6121 cgggaatacc ggatgatagt ttctttcgat ttacttccgt aaggcactct cgccgtatca
     6181 ccaaaatata cgattttttc tgaaggaaga ttacgcatga tctcacgcgc aacagtcagc
     6241 cctcccacac cggaatcaaa tactcctatc ggtgcctctc tgtctatttt cattgatttc
     6301 tattctccat ctctattgat tgtctcacta ttctatcacc aatcaagagc ctgtgcaagc
     6361 cagaaggata tctttatgtc atctttctgt cctttatttt cctcagggaa ctgcgagaag
     6421 cagaatttcg ggtacaatgt cccccaccat gcagtaactg cagtctgttc ctgtcgtctt
     6481 tcagcgattt ttgctatctt cccggacatt cccggcattg ctctcatcat aagaacacca
     6541 acagctgcca cgaacagaac aaacgacagt tttcttctta attttttgtt cttactcttt
     6601 ttattcatca ttgtccctct ctcctgttat atcttatcag ttaccatatc tgcctttgtt
     6661 attttcctat atattttctt attttatatg aaatttccag acagcacttt aatatttgga
     6721 ttatgccaat attttcctga tttatacttc cacttccaga cttccatttc tgatcttcac
     6781 aatcggaagt cgcaggattt tatcttcttt atatttttca tagaaaagct ctctgagtgt
     6841 cacagccgta atatttcctc ctgacattct gtttcggatc agtcctgtaa tatattctcc
     6901 tgctgatgaa ttatcctctc catgccagtt caggatctcc cctgcgtcct ccgcttcaaa
     6961 cacataaaga tcttccccca caaaaatctc ctgtttcaga taatccagca ccattctcca
     7021 tcttccgtcc tccaccagtg ttctccccat gaccagaacc tgcagatgcc ccatatcaag
     7081 aagtttttcc tgtgactgat catacatttt ttcaatccgg gtgaaatcag caccactgat
     7141 ctgcagaacc cgggaaccgc catcttcttc ctcttttccc tgccccgtac tctcagaaag
     7201 atccggcatc ccataggtaa gacagattcc ttcttctgag gcatccacgc caagtgccat
     7261 aggatacatt ctcttctccg gttccacagc cgcaccacat ccgctcataa aaagactgac
     7321 tgacaaaatc ccggtcacca cactgacagc ccttttcctc tgcttcctgc ggtgtccctt
     7381 tcctctgata aacatataaa actgacagat gagaattccc gggacgaaaa tatgcgccaa
     7441 acaccatcca aaataatcca gtattccttt tccctcttct tccagaagcg aaatcaggaa
     7501 aaccagtgcc ggaagccaga acctccccct accaggatga gatttcccga taatctgatt
     7561 tccataatac agcagactcc ctattgcaaa aagcaggctg taaagcagaa atcccatcca
     7621 caggatgtca aatctggcca gtacatttcc cggaagatct gcgcctgcca gcagaggcag
     7681 gaccggataa ctttccgccg ctaccctgtc atatcccaga actgccggaa gcagaagctc
     7741 cattccaata agaatcccgc caagtgtcag gatccctcct gccaccgtct ttcccgcact
     7801 tccgtatttc tccacatttc ccaacagaaa tggcagcaat gccaccggcg aaaaggcaca
     7861 cagagttcca taaaaagact gacggacatt tctcactgta agctcctccc accggattcc
     7921 tcccacgaga tattccgttt ttgcctgtgg cacacaaagt atcatcatta tcataatccc
     7981 gccaagaagc agaccacctg atacttccgc cattcttcct cttctctgta cacctttgca
     8041 ggttccgaca gaacatacca gaaccgccca gaatgcgatc catcggccgg atactcctgt
     8101 gatcagcttt acaggaacca gcctcctgag aagtgccagc agatatcctc ccgccatcaa
     8161 cacatagatc agaaaaaaaa tcccgatcag ccttcccgca aaagcacctg cagactttac
     8221 cggatcttca aatgaaggtg tcagccgtat cagccagatt acataaaacc caagcaatat
     8281 cagcgcaaaa atcattcccg ccactgcact gattccgttc attccaccct ttccgaaaac
     8341 gcagagcata aatggtgcca gtaatgccag tatcatctgc ctgtacagct gcctgtggga
     8401 aattctgtta ttctctgcaa atctcatgaa ccggactcct ttctcttcaa acggatcttc
     8461 tgctcctctc ttgcataaag cggtcgtaat accctcttgc gcagcggaac tctcagaacc
     8521 ccttctcctc tgctgccctt ctgttctttc tttataaaag gaaccaggta aggaatacca
     8581 aaacttatca ggcctgccag gtgccctata accagatata ctcccagcac gattccgtaa
     8641 attcccagat aacctcccag gatcagaaac ccatacttca ccagccgaaa tgctgctgca
     8701 aactcttcat ttggaacagt catagagcca agcgcagtca acgccacgat catcaccacg
     8761 atcggactca caagatttgc ctctaccgct gcctgtccga tcaccagtcc gccaacaatg
     8821 ccaatagcat ttcccagaga tcccggaact ctcaccccag cctcccgaat cagttcaaaa
     8881 gccagttcca gaaaaatcag ttccaccaca ctggaaaagg gcactccttc cctcgcctcc
     8941 gcaaaagaca gtatcagtgc agatggcaga atctgtgtat ggaaacgtat cactgccagg
     9001 taaagtcccg gcagaacagt cgccatgatc acagccaaat atcgcagtat tctgagaaag
     9061 gaagccatct cgaacctgtg ataccagtcc tcgctgctct ccatgaaact gctaaaattt
     9121 cccggaagga tcaatgcttc cggagaatta tcacacagaa tcacaacctt acctttcagg
     9181 atttcctgca cagcacgatc cggacgttcc gttgtctgat actgaggaaa aggagaatac
     9241 cacacatctt ctgtcagctg ttcaagcata ccgctgtcaa ggattccgtc aatctgaaat
     9301 tcttccagcc gttccttcac ttcttccaga agtccctcat gtaccaggtc atccatgtat
     9361 aacacctgcg ttaaggtatg agagcgcaca ccgattttat attcttccac cttgagtctg
     9421 gtatcacgaa gcctttttct taccagtgca ctgttacttt tcacactgtc cgaaaatcct
     9481 tccctggaac cccgcagcac cttttccgat tctgcctcca tcacacctgt ggatggataa
     9541 cctttgctgg agattttcat cgccttgtca tatccatcta tgaaaaacac tgcattccct
     9601 gcaagcatgg catctatgga ctcgtcaaga ttctccagtt tctttacatc tgcgatccca
     9661 agactgttat gcctgacaaa ttcctgaata tcctccggcg aaatctccca gaaatgattg
     9721 atcatctttc caagagcaga atcatccagc atcatgttgg aaaccgcaac ttctatatag
     9781 accataaggc agtccacctt atgtttatct cccagccgca tgggacggat cagaatgtca
     9841 ctgcaatttt ccagcctgct tcgcagatat ttctcattct ccctcagatt tgcagatact
     9901 ttccgggatt cttccagttt cttcaataat ttcccacctc gtttcatacc ggtacatcgt
     9961 tttcgatgta ccagacagat ctgttgccag tcggcaatat tcctaaaccc acttaaatac
    10021 atggatgcgc aaaaaaagag aggcatcctg cctctcttag aatttcccga tattcagata
    10081 ttataccatt cttatctgtg tcccctggat ttctctttct ctgcacgctc acgttctgca
    10141 tcctcagcct caatagaacg catgatagcc attctctgat tctcgatatg ctggaaagaa
    10201 agctgtttcg cgcgttctac gtcacgctct ctgatcgcct ttgtcagttc ctcatgttcc
    10261 ttcaccagaa catctcgtgt ttcaccttct ttcagatatt ctacacgata acgataaatc
    10321 tgctcacgca tattattgag tacctggatc agcctcttat tatcagaagc ctgataaata
    10381 acctcatgga atgcaacgtc agcctccgca agcttaacaa gatttccctc tgcgatcgta
    10441 tgttcaaact ccttggcagc aagccagagt ctcctcattt cttcttcagt catacgggca
    10501 catgctttct caatagctac attctcaaga gcaatacgaa cctccagaac atcattcaga
    10561 tccttctctg tgatattggc aacctgggca ccacgtctcg ggatcatcac taccagacct
    10621 tcctgctcca gcttacgcat tgcctctctg ataggagttc tgctcacgcc aagtctctct
    10681 gccagcgcaa tctccatcag tctctcacca ggtttcagtt cgcccttcag gattgcctga
    10741 cgcagcgtat taaaaaccac atcccttaat ggaagatatt cattcatatt tactgaaaaa
    10801 tcacttgtca tttgtattcc ctcctgcttt cgttacttct gtggcaaaaa ccgtcttcgc
    10861 cagccggctc tctctgagaa cctctgctgc cgcctccatc ttctcacgat catcaaaaat
    10921 tccgaacact gtaggaccac ttccactcat catcgccttt aacgcaccat gactctccat
    10981 cagtgctttg atttcgccaa ttacaggata tttctctata attcccggct caaaaacatt
    11041 tcccatcttc tgtgtcattg ccagaagatc tccacgttct atatctccta tcatcccgtc
    11101 aatatccgga tgagaagaaa tcttatccag ctgaaggctc tcataggcag ctttggtgga
    11161 aacattgatt ccgggcttac cgatcagtac aaaacagtcc ggcacctgag tgatcctggt
    11221 caatttctcc ccgatcccct ctgccagggc agttcctctc atcacacagt acggaacatc
    11281 tgccccgata ttcacagcac gatccatcag ctccttctgg ctcagtccca gtttaaaaag
    11341 ccggttgatc cccaccagtg cagctgccgc atccgagctt ccgcctgcca tacctgctgc
    11401 cacaggaata aatttctcaa gatggatatc cacaccaccc ttaacctgaa attcatccat
    11461 cagaagcttc gccgccttat acaccagatt tcgttcatca ttcggaatat aggaataatt
    11521 gacagacagg gaaatccccg gcttcctcac cctcttcatc tccagaacat catacatctg
    11581 tatcgtctgc atgatcatcc ggacttcatg atagccatcc tctctctttc tgagaatatc
    11641 cagtcccaga tttatcttcg ccaatgcacg taaacgcata aaatcctccg tttccaacaa
    11701 tatcatccgt atataactgt actttcgcaa ttatatttgt actttgtata caataaaact
    11761 ctttgcgcat attttaaatc attttttttt agttttcaat ataggagttt tcattttttc
    11821 cggaaatttt tttcattatc atccctgcct gacaagaatt aacgactgcc ccggctttac
    11881 ctctttttcc gtaagactgt tcacacccga aatctcatcc acagttgtat aaaatttctt
    11941 tgcaatatcc cacagggtat ctccggcctt tacaatatag accgtaattc ccggcatact
    12001 ttccagtttc ttacgatcca gtggctgttc ttccacagat tcaataagct gctcttccca
    12061 ctgtctgaac acaagcacat tcagtcccac cgccgccttg acttcaatct catccccatc
    12121 tgccattgca gtggaaagct gttcaaggtc agcctgaagt aaatatgtgc aatccttccc
    12181 gatctcttct gcctcgatca gatgggtaaa cggaagcatg gcatccatag aataaaacgg
    12241 catttcatca tttccaataa tatacaggat cttgagtgcc acaattcctt ctgccacaat
    12301 tcccttatcc gtaatcctgg tcttatctac ttttacctta ccgctgctgt ggcaaagctg
    12361 caaaactttt ccctgactct ccttaacttc tatccggtct gtcagacgac acctggaaaa
    12421 attccgtacc agaagacttt ccaacatttc ctttttccga tgaagaacac attctttgtg
    12481 tgggctgtat gcatcaagaa gaagttcatg ctcctcttcc ctgtacattt tcatatttaa
    12541 ttccagtacc acatccacct gcaggatacg ttcttcacca tccggatcag gctttacctc
    12601 tatcccctga tgaagaagag acacctcaat atgtgggatc agatcttcca tacagccact
    12661 gcattccatc tcattggaaa aaggcatggt atactccagc cactgaggtg gattctcctc
    12721 ttcataccct gtatacagaa gaaaaactgc aagctctcct ttcacccgga gtttgttctc
    12781 tcccggccgc agatccagat tcctcggttc agccatatac cacaaaaggt tttctacatt
    12841 aggtctgttt gacgcaagag taatatcatc cttaatgcgc agagtatctt ttttatgtac
    12901 acgaagactc ataagccgga ctttctccgt tttcacagag acttcctgat cctctgcgga
    12961 aaccggaagt tcaacgactg ccagttcatc taccactgca taaaaagtta caattgcttt
    13021 aatattcagt tttctggaat ggatcatatg aacactgaga tcctcaatct cccatttcag
    13081 acacagctta tctccccctt cgattccctc cagattaatc atctcatcca gcggaagctt
    13141 cgcagaaagg ctgtaaatcc tgccctcttt ttcccccaca taaagcaaat ctgcatttaa
    13201 tgtcccttta agaagcgctc ttccttcact cagcctcact tcctccatgg aaacatccgc
    13261 tttgctctgc accagtctcc cgatatccgg tttcacatcg gacacattat agtcctcgtc
    13321 aaaagtcgcc tgcgctgccg ctttattttt gatacgcagc atctgaatac tttctttttt
    13381 taattccaac ctgcttcctt cctttctctg tttactttat gataaaaccc aaaaattcta
    13441 tcggtttctc tgtttctctg attttcagga caatacaggg ataagacggt tcactgcctg
    13501 catccgcttt ctccggacct atcagccttg tcttgcagaa tactgcgttc tctgattcgg
    13561 aaacctcctc cacctgaata ctgtaccctc ctgtaagctg ctctccatat cccttcatca
    13621 gataccttat atctcccacc tgataggtca tctgaaatac tttctttttc ttctgttcca
    13681 tcagttctga aatctcttcc ggaatttcct cctgctttac gatcgtatat tccagaggtg
    13741 tacgttcgcc ctcctcaatc ctgataaact gacagcccgc cggcaaaaga gccaacagca
    13801 acacacagag cagtcctcct gtccttttgc ccataatgca cccctgctca ttcttcgcta
    13861 aatattctat gccggataca gccctgttat taccatactc tatgctatat atccgacagt
    13921 aactgctttt ctgcaggaaa gaatcctgtc ctgagtgctt tatttaatgc caaaaaatca
    13981 ttatactatg atttatttct tgcaggatat ctcataattc cttataataa cttctatact
    14041 tatatatggg catcagcatt atataatgcc cggaaaggaa ttaacgaatg atacgattta
    14101 tcctagtctg tattgttgtg atcggatacc tgatcctgtc catccccatc ctgctggtcg
    14161 aatggatcat cggcagattt tctcctgaaa agaaggatat cagctccctt aggatcatcc
    14221 aggcagtatt ccgatttatc cttaagatca ccggtgcaaa gattactgtg atcggagaag
    14281 aaaatgtgcc aaaggacaca cctgtacttt atatcggcaa ccacagaagc tacttcgaca
    14341 tacttcttac ttactcccgc tgtccgatcc gtacaggcta cattgccaaa aaagagatgg
    14401 agaaaattcc tcttctttcc acatggatgc gttatctcca ctgtctcttc cttgacagga
    14461 aagacatcaa acagggactc aaaacgatcc tgaccgcagt agacaaagta aaatccggaa
    14521 tttccatctg tattttcccg gaaggaaccc gaaacagaaa taaagatgag ctggacatgc
    14581 ttcccttcca tgaaggaagc tttaaaatcg ctaccaaggc caattgcccg atcatcccaa
    14641 ttgcgatcag caattcagcc aacattttcg aggcacactt cccgaagata tctcctgcaa
    14701 aagttgtagt agaatacggt aagccaatct atccggatga actgagcaaa gaagataaga
    14761 ggcatgtcgg agaatatacg caaaacgtaa ttcgcgaaat gcttataaag aacaaaccac
    14821 tgaccgaaaa ataataagac tatcaccagt ccggcagagc gtaaagacaa aaatttacgc
    14881 tctgccggac tgttatatat cagaatttcc attgtatctg gactaatccg ggaaatacct
    14941 taagtgacct gatcatctta tcaactgtaa acctcttctc ctcaaaatca atctctcccc
    15001 acagacttat attagatgct accttttcca tctgctccgg tgtcgcatga ttctccgcaa
    15061 gcaccgataa tttctccagc tttaactgat gcatctggtc tatttcttcc actctctgat
    15121 tgatataatc tgtcagtgtt tctccggcac ctgccagaga atccacaagc agagaaatct
    15181 ccttttccat ttcctcaatc tccctgtaaa tctgctttac ctgcggattt tcatttaact
    15241 tcgtataagt ggcaggagac tgtccttcac gcagcttacg taccatctct ttatatacat
    15301 aattctccac atcttcagta taaaccttcc cgcaccccgg gcaggctttg ttgttcagac
    15361 gcttcgtaca tctcaggtac tgtttaccgg actgtatttt aatactcata agtgcgtatc
    15421 cgcaattgcc acatttcact ttccctgcca gccatgtatt cacagctttc ctattggcct
    15481 gaatcgtttt atttctcata agcttgatcc tgcagttcag ccactgctcc gaagacacaa
    15541 gcccttcatg aggtgccacc accagcatat gacccttaag attctgcagc ttatctgtct
    15601 gcgcatcccg cccctgataa agataacatc catgaatccc gtcaaactgc tccggactgc
    15661 ttactatatt ggttccatgg ctcctgaaaa aacggtacac atccatatca gcccgcacat
    15721 ataccggatt tctcaaaagc tgggccagca tggaacgtat cagttccttt ccgtagaact
    15781 gcactccctt ttccgcataa tatctggtga tatccccata agaagtgtcc ggttgttcgt
    15841 acatatcaaa aatttcccgc acaattgccg cttccccggg ttcttccaca agcttctttg
    15901 tatggattcc ttccatcagg atctcctcaa gccgataacc ataaggaggc ttgccgccca
    15961 tgcgaaaacc tttctgactt ctggaataat atgcgtcgct gacacgcttc tgaatagtct
    16021 cacgttccag ctgcgcaaac acaatgcaga tatttaacat tgcctctatc tataaaggtg
    16081 tggttaactc tgattctttc tccgtccacg actttgatat cctgactctg tatccactgg
    16141 attttgaaga atttctgtgg gccaatgctg agtttgccct tacaagagaa atccgcaatc
    16201 atttctccga tttcagctca atgggaaaga aactccacga aaaagccctg tcacagttcc
    16261 gtctctatct gatcatcggc ggaatgcctg ctgccattat ggaatacaaa aaagaaaaga
    16321 agctcctgct ggtacctgac acctagcaaa agctccttac atctgtatcc tgttgacacc
    16381 ggattatgct catgcatact gaaaatccct gccttccagc ttctgtcagg agaagaaacc
    16441 accgccggta cagcctgtat cgaaaccttt ctggcacagc actttatccg gaatggctat
    16501 gccctctcct actggtccag cggcaaccag gcggaagttc cctttttact gtccaaaaac
    16561 agtcatttta ttgccataga ctaccgcacc accccacatc agaaatgcag gaaccttatg
    16621 cagctgaata aatcttctct ctctcctgca cccacgcaaa tgtatttaat ctctgccgaa
    16681 gacttcaggc aaaaagaagc ttatcagatc gtgcctttat atgcggtatt ttgcatatag
    16741 aacgtggcag atacaacagg caaaagcaga ctccggccga tagttcttct cgctttcaca
    16801 tcttcggaaa cgaagaaaaa acatcttcag ccatatctct ctgatatggc tgaaaacttt
    16861 ttacgcattg ttaaggctct tcctgataga gttttccatt taccagtatt tttttacata
    16921 attaacagcc tcttcctgtg acagggaaaa ttcattcatg atgtttctga ctgtatctgt
    16981 ctcacttata ttaaaattcc tgcaggtcct tacagtcgca cgaatgcctt caaggatacc
    17041 ttcccatctg ccttcctgac ggccttcctg tcgaccttcc tgtcgacctt cttcaacacc
    17101 ttcctgtctt aactcttcca atgcattaca catattgatc tgtccccctt tctgctccga
    17161 ttccaaagca tggtcaatca gtttctgtga ttctgtaatg gctcctatca caagtccaag
    17221 atctgccgga atattatgat ccctgtaaac ttcattgatc ttcgtataat ccctggcata
    17281 gatcaagcgg ctcacgtcaa acaccatatt tacgtccgga tcgctgaaac atagttctcc
    17341 acttgatctt acctgtacca ggttcatacg gtaatcagat accagtgatt ctatcttttc
    17401 ggggatctcc agcatatcct tcagggaaaa cggaccatcc cattcacttt ctccataata
    17461 tacacacaga ctaatcaccg gatgcagccg gtcggtcttc ttaaaacctg acaggaattc
    17521 atccgctgaa tggaaatccc cgtttttctt atttactgct gcaatctcat catattcttt
    17581 catgtaagaa aatgcatctc caaccatatg gcgcagcggc atcgcatagt ggatctttgc
    17641 ctgattttcc agtccccaga gaacaaagtc cacaccatat gccgtcttct ttacaacatc
    17701 caggatcttc tgtactgttt ccgcatgacc attaaatttc agcatggagg aaacatccgt
    17761 atctgcttct gtaagatcct gcggcttcag cacctgttcc ccgttaaaaa atactgcatt
    17821 gaacagatcc gcaaaatgtc tgttgtctct ccagaagttc ttcagtatgg tatctggttt
    17881 gatcttattt ttttccgccg tatccagcac ctcccgttga tttatttctg ttatcaatat
    17941 agcataatta ttaccttgtt tccacgaaag gaactataaa aattattaaa aatctgaaaa
    18001 gtgcagagtc gatttgttta atttgttctt ttttttattt cgtcagctgt aaaagagcat
    18061 ataactacaa aaataccgct ctaatacgta aaaaaggcag ttataaataa ctattttttc
    18121 agggtcgtgc atgaatttct aatcagagaa tgtgcttttt aatcaaaaat taacctatcc
    18181 actacccaaa ggggaactat aatcatgaca aagattaaaa acacaaagaa gggcgtggca
    18241 aaaaagacat taagtatgtc acttgttgtt gcaatgcttg ctacttctaa tgtgccggta
    18301 tgggctgctg agttcagtga tgattttgat gctgctgtag ctactgaagc tccggttact
    18361 gaatttagcg ataatacaat agaaactcca gctattgaag aagaaacaac agaagctcca
    18421 atggctcagg cagttgttga atctgatgat ctcagtgttg atgtttcagt ttctgaatct
    18481 gccgaattta ctggtattgt atccgtttct ggtacaatta atgcaagtga tctggataaa
    18541 tggggtattg acaaacagac aattgctaat tatgatgtta ctattaaaga tggtgtagca
    18601 actgtaatta atgatactat caaagttcca gtatctgaat atacagttac aaagaatagt
    18661 gatgatactt atacagtagc tgccaaatct acatctaaga actatactgg ttcaa
//
LOCUS       DBMDXG010000006        16926 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1021_length_16926_cov_1.511263, whole genome shotgun sequence.
ACCESSION   DBMDXG010000006 DBMDXG010000000
VERSION     DBMDXG010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 16926)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16926)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16926
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1021_length_16926_cov_1.511263"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="ACZPD3_00525"
     CDS             <1..456
                     /locus_tag="ACZPD3_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR1414538.1"
                     /translation="RVLIAGVEQLWDAGLVVVAAAGNQGPKARSVTAPGSSRKIITVG
                     SSDLLTGRTAISGRGPTFECVCKPDLVAPGNHVLACAPGADNGYGVKSGTSMSTPLVA
                     GAAALMLEKNPELTNVQIKMKLKESARDMGLPKNQQGWGELDLERFMEL"
     gene            572..1780
                     /locus_tag="ACZPD3_00530"
     CDS             572..1780
                     /locus_tag="ACZPD3_00530"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR1414539.1"
                     /translation="MKKIQMQTPLVEMDGDEMTRILWKIIKDELLLPYIDLNTEYYDL
                     GLEYRNETDDQVTVDAAEATKKYGVAVKCATITPNKARMEEYTLKKMYKSPNGTIRAI
                     LDGTVFRAPIVVKGIEPCVKNWKKPITIARHAYGDVYKNTEMYIDGPGDAYLVFEGAD
                     GQQRKELIHHYEGPGVLQGMHNLDDSITSFARCCFNYALDTKQNLWLGGKDTISKIYD
                     GRFKEIFATIYEDEFKEKFEAAGIEYFYSLIDDIVARVMKAEGGFIWACKNYDGDVMS
                     DMVSSAFGSLAMMTSVLVSPQGYYEYEAAHGTVQRHYYRYLKGEETSTNSVATIFAWT
                     GALRKRGELDGNKELMEFADKLEKATLETIESGKMTKDLALITSLEDVTVLNSKDFIL
                     AIRERLDEIL"
     gene            complement(1898..3808)
                     /locus_tag="ACZPD3_00535"
     CDS             complement(1898..3808)
                     /locus_tag="ACZPD3_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1414540.1"
                     /translation="MILSCQNICKSFGEKIILKDASFHIEDREKAALIGNNGAGKTTL
                     LRIIMHELSPDSGNVVLAKDKNIGYLAQYQDIHGHHTIYEELISTKQHIIDMENRLRS
                     LEQEMKTTTGDALDSLMNTYTRLSHQFELENGYAYKSEIMGVLKGLGFTEEDFERQIE
                     TLSGGQKTRVALGKLLLASPDILLLDEPTNHLDMESISWLETYLLNYPGAVFIVSHDR
                     YFLDKVVTKVVEIEAGNMRMYSGNYSAYALKKAQLRDAQYKAYLNQQRDIKHQEAVIA
                     KLKSFNREKSIKRAESREKMLDKVQRIDKPQEIQNQMRISLEPRFVSGNDVLTVESLS
                     KSFPGQTLFSDISFEIKRGERVALIGNNGTGKTTMLKIINGLIDADSGRFTLGSKVQI
                     GYYDQEHHVLHMEKTIFEEISDAYPTLTETEIRNMLAAFLFTGDDVFKLISALSGGER
                     GRVSLAKLMLSEANFLILDEPTNHLDIASKEILEEALNSYTGTVFYVSHDRYFINQTA
                     TRILDLTNQAIVNYIGDYDYYLEKKEELTEKYAPAAAQAVTEAKQASDNKLSWQQQKE
                     EQARQRKRENELKKVEARIEELETRDKEIDETMILPDICTNVAECAKLSKEKAAITEE
                     LEGLYEKWEELA"
     gene            4069..4716
                     /locus_tag="ACZPD3_00540"
     CDS             4069..4716
                     /locus_tag="ACZPD3_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR1414541.1"
                     /translation="MEAKEISKAVIKRLPRYYRYLGELMEENVERISSNDLSKKMHVT
                     ASQIRQDLNNFGGFGQQGYGYNVRYLYTEIGKILGLDTVHPMIILGAGNLGQALANYV
                     DFEKRGFKLVGIFDVNPVLEGIAVRGIEIQMLSDLPLFLKENEVEIAILTLPKNKAKE
                     MAKILIDNGIKAIWNFAHIDLDAPEDVMVENVHLSESLMTLSYNLSEYRKEHEGM"
     gene            4806..6305
                     /locus_tag="ACZPD3_00545"
     CDS             4806..6305
                     /locus_tag="ACZPD3_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HIR1414542.1"
                     /translation="MREIVTAVEMKELDHNTIQKAGISSPVLMERAALKTVEEIVQRL
                     KNKEKEKILVVCGTGNNGGDGLAIARLLQLRGVRTWYYIAGKEEKMTAETAGQLKTAE
                     YYQVPRVHNLDPDEYTTIVDAIFGVGLSRPVEGKYAELISTMNRASAYKVAVDIPSGI
                     DSDTGFEKGIAFRADLTVTFAFRKRGLCFYPGRMYGGEIVVTDIGIYSIPGKKICMHH
                     LEREDLKMLPERVPYGNKGTFGKVLLIAGSEGMCGAAYLSAAAALKSSAGMVRIQTVE
                     ANRIPLQTLLPEAMITCDFDEKKNQAMLDWCDVLVIGPGLGTGAQSRERAQWFLAKAA
                     ECKKTVILDADGLNLLSVHDNWKCFIGEHVILTPHIGEMSRLCGKEIGKIQDCLAQTA
                     ADYAKESGAVCVLKDACTVVADAFGDMYLNISGNAGMATAGSGDVLSGVLAGIQCMYL
                     AAEKKFSPVMLAALGVYVHGLAGDLAAETVGQRGMTAGDIICFLPEILR"
     gene            6383..7528
                     /gene="alr"
                     /locus_tag="ACZPD3_00550"
     CDS             6383..7528
                     /gene="alr"
                     /locus_tag="ACZPD3_00550"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR1414543.1"
                     /translation="MKKDSRVKAVISLDAVEHNFREMRKNIAEETKMIAVIKADAYGH
                     GAVPVAHLIEDYDYIWGFAAATAEEAIHLREAGITKPILILGIVFDEYFPELVRYEIR
                     PAVCEYEEARKLSDEAVLQKKTVHIHIALDTGMTRIGFADAQENVEEIKKISELPNLE
                     IEGMFTHFARADEYDRSPAMVQLERYLDFSRRVEEAGVEIPLHHCSNSAGIIRMPEAN
                     LNIVRAGITIYGIYPSSEVERDIVKLEPVMELKSHVTYVKDVPAGAAISYGGTYVADR
                     KLRVATVPVGYADGYSRQLSNKGWVLIHGQKAPILGRVCMDQFMVDVTEIGDVKKGDE
                     VTLIGRDGDEFIGIEEIGDLCGRFSYEFACDISPRVPRVYIKNGKEI"
     gene            7714..8061
                     /locus_tag="ACZPD3_00555"
     CDS             7714..8061
                     /locus_tag="ACZPD3_00555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1414544.1"
                     /translation="MVIKRGDIFYADLRPVVGSEQGGIRPVLIIQNNVGNRHSPTVIC
                     AAITSKMNKAKLPTHIEINAELYGIEKDSVILLEQLRTIDKRRLKDKVCHLDDAMLDK
                     VNHALEISLELTF"
     gene            8262..9575
                     /locus_tag="ACZPD3_00560"
     CDS             8262..9575
                     /locus_tag="ACZPD3_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR1414545.1"
                     /translation="MEDGSSMPLWGLFVLLLLLWLNGIFYGFAAALRNISENDTQKKA
                     EEGDKKAQMLMTLIDKPAQFVNVIPLIVMARGICFGTFLVPYAVDAFYPYIKHVPALI
                     LVMALCVIFLAAIGILAFRRVGTYHPEAYAYKYLNLVHFWLNLLKPFTVFVTWIARLA
                     AVPFGVEINRTEKSVTEEEIISIVDEAHEQGVIQENEAEMIQNIISFNETEAHDIMTH
                     RKNVVAFDEEILLKNMIDTMLEEGNSRYPVYEENIDNIKGIVHYKDALKFMTQNPWAK
                     FKPLKELPGIIRQASLIPETRGIGDLFHTMQARKIHMAIVVDEYGQTAGIVTMEDILE
                     EIVGDILDEYDEDEITIRAQKDNSLIIDGLAYLEDVGEELDADFGDTEFETLNGYLTN
                     ILGHIPTDKDVDTSIKAIGYCFTILSIGNKTIGKVKVERDNNVAV"
     gene            9617..10348
                     /locus_tag="ACZPD3_00565"
     CDS             9617..10348
                     /locus_tag="ACZPD3_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1414546.1"
                     /translation="MSGHSKFANIKHKKEKNDAKKGKIFTVIGRELVMAVKAGGPDPN
                     NNSKLRDVIAKAKANNMPNDTIERGIKKAAGADSADNYEKAVYEGYGPNGVAIIVETL
                     TDNKNRTAGNVRNAFTKGNGSIGTQGCVSYMFDQKGQIIIDKEECEMDADEIMMLALD
                     AGAEDFSEEEDSYEILTAPDDFSAVREALEKAGLPIAEAQVAMIPQTYVELTDEQALK
                     DLQRTLDLLDDDDDVTDYWTNWDEQ"
     gene            10459..11211
                     /locus_tag="ACZPD3_00570"
     CDS             10459..11211
                     /locus_tag="ACZPD3_00570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0051117 - ATPase binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP family protease"
                     /protein_id="HIR1414547.1"
                     /translation="MSENRYGEKREESETGMKLQNCETQESENKVQESTNEQIKDMGQ
                     VVLNSNSRKHKVELLTIIGEVEGHESAPSHSKTTKYEHVLPKLAIIEDDEEIEGLLIL
                     LNTVGGDVEAGLAIAEMIASLSIPTVSLVLGGGHSIGVPMAVSADYSFAVPSATMVIH
                     PVRSNGMFIGVAQTYRNMEKIQDRITTFISEHSRASQKRLEELMLDTSQLVKDVGTML
                     EGKEAVKEGLIDEVGGIKEALAKLYEMIEKEC"
     gene            11312..12199
                     /locus_tag="ACZPD3_00575"
     CDS             11312..12199
                     /locus_tag="ACZPD3_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR1414548.1"
                     /translation="MYKILKAETLAAKIHLMVVHAPRVASHCQPGQFIIVKLDEKGER
                     IPLTICDYDREEGTVTIVFQEIGVSTIKMAELKAGDAFRDFVGPLGCPSEFVEEDIEE
                     LKKKKMVFVAGGVGTAPVYPQVKWLHEHGITADVILGAKTKDMVILEKELGEVSNLYV
                     TTDDGSYGRAGMVTKTLEDLVTNEGKHYDVCVAIGPMIMMKFVCLLTKKLGIHTIVSM
                     NPIMVDGTGMCGACRLQVGDEIKFACVDGPEFDGHLVDFDQAMKRSQMYKTEEGRALL
                     KLQEGDTHHGGCGHCGGDK"
     gene            12199..13593
                     /gene="gltA"
                     /locus_tag="ACZPD3_00580"
     CDS             12199..13593
                     /gene="gltA"
                     /locus_tag="ACZPD3_00580"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR1414549.1"
                     /translation="MADAKMLKKVPVREQNPKVRATNFEEVCLGYNQEEAMEEAQRCL
                     GCKKPKCVEGCPVSINIPGFIEEIKEGKIEEAYKVIGLSSALPAICGRVCPQESQCEG
                     KCIRGVKGEAVSIGKLERFVADYALEHDIKPVGAEVKNGHKVAVIGSGPSGLTCAGDL
                     AKAGYDVTVFEALHELGGVLVYGIPEFRLPKQKVVKKEIEKVKELGVKFETNVVIGKS
                     TTIDQLIEDEGFEAVFIGSGAGLPMFMGIPGENASGVFSANEYLTRSNLMKAFDDSYD
                     TPIAAGKKVAVVGGGNVAMDAARTALRLGAEVHIVYRRSEAELPARAEEVHHAKEEGI
                     IFDLLTNPKEILVDENGHVKGMKVVKMELGEPDASGRRRPVEIPGSEYDMDVDTVIMS
                     LGTSPNPLISSTTKGLEVNKRRCIIAEEETGKTSKDGVYAGGDAVTGAATVILAMGAG
                     KAGAKGIDEYLKNK"
     misc_binding    13653..13884
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14068..15087
                     /gene="pheS"
                     /locus_tag="ACZPD3_00585"
     CDS             14068..15087
                     /gene="pheS"
                     /locus_tag="ACZPD3_00585"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR1414550.1"
                     /translation="MKERLQELAEAAGKRISESEGLEKLNEVRVAYLGKKGELTAILK
                     SMKDVAPEERPKVGQLVNETRSKIEALLDESKQKLEEAAREEKMKQEVIDVTLPAKKA
                     KIGHRHPNTIALEEVERIFIGMGYEVVEGPEVEYADYNFTKLNIPENHPARDEQDTFF
                     INDSILLRSQTSPVQARTMEKGKLPIRMIAPGRVFRSDEVDATHSPSFHQIEGLVIDK
                     NITFADLKGTLQEFAQELFGPETKTKFRPHHFPFTEPSAEVDVSCFKCGGKGCRFCKG
                     SGWIEILGCGMVHPNVLSMCGIDPEEYTGFAFGVGLERIALLKYEIDDMRLLYENDIR
                     FLKQF"
     gene            15192..>16926
                     /gene="pheT"
                     /locus_tag="ACZPD3_00590"
     CDS             15192..>16926
                     /gene="pheT"
                     /locus_tag="ACZPD3_00590"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR1414551.1"
                     /translation="MNTSLSWIKMYVPDLDVTAQEYTDAMTLTGTKVEGFEKLDADLD
                     KIVIGQIEKIEKHPDADKLIICQVNIGTETVQIVTGAPNVKEGDKVPVVLDGGRVAGG
                     HDGKKTPGGIEIKKGKLRGIDSYGMMCSIEELGSTREMYPEAPEYGIYIFPEDAVVGE
                     SAIKALGLDDVVFEYEITSNRVDCYGVLGIAREAAATFNEKFCPPVVECKGNDEKASD
                     YVKVTVEDPKLCPRYCARVVKNVKIGPSPKWMQRCLASNGIRPINNLVDITNYVMEEF
                     GQPMHAYDLDRIAGKEIVVRRAENDEKFVTLDGQERTMDDQVLMICDGEKAVGIAGIM
                     GGENSMITDDVKTVLFEAACFDGTNIRLSSKRIGLRTDASGKFEKGLDPNNAQAAIDR
                     ACQLMEELGAGEVVGGMVDICNEVREPSRVPFKPEKINALLGTDLTPEEMLAYLAKVE
                     LTYDEKTNEIVAPTFRQDIHYVADVAEEVARFFGYDKIPTTLPTGEATTGKLPFKLRI
                     EAVARDIAEYCGFSEGMTYSFESPKVFDKLRLPADSKLRQGIVISNPLGEDYSVMRTT
                     TLNGMLSSLATN"
BASE COUNT         5021 a         2982 c         4348 g         4575 t
ORIGIN      
        1 agagttctga ttgcgggtgt ggaacaactt tgggatgcag gtctggtggt agtggctgcg
       61 gcaggaaatc agggaccgaa ggcaagaagt gtgacagcac cgggaagcag ccggaagatt
      121 atcacagtag gctccagcga tctgcttaca ggacggactg ccatatccgg caggggacct
      181 acctttgagt gtgtttgtaa accggatctg gttgcacccg ggaatcatgt tcttgcctgt
      241 gcaccgggag cagataacgg atatggagtg aagagtggta cttctatgtc tacacctctg
      301 gttgcagggg cagcggcatt gatgcttgag aagaatccgg agctgactaa tgttcagatc
      361 aagatgaaat tgaaagaaag tgccagggat atgggattgc caaagaatca gcagggatgg
      421 ggagaactgg atctggaacg ttttatggaa ctgtgatcag taatctttta tttcacaaaa
      481 ttattgcttt agtgtataaa agcattgacg aacggtctgt tttcggctat catgatagtt
      541 aactggatga gagccaaaga ggaggattaa catgaaaaaa attcagatgc agacaccact
      601 tgtggagatg gatggagatg agatgaccag aattctctgg aaaataatca aagatgaact
      661 tcttcttccg tatattgatt tgaacactga gtattatgac cttggtctgg aatacagaaa
      721 tgaaacggat gatcaggtaa ctgttgatgc agcagaggct acaaagaagt acggcgttgc
      781 agtgaaatgt gccactatca ctcctaataa ggcaagaatg gaagagtata ccttaaagaa
      841 aatgtacaag agtcctaacg gaacgattcg tgcaattctc gacggaactg tttttcgtgc
      901 cccaatcgta gtgaagggta ttgaaccatg tgttaagaac tggaagaaac cgatcaccat
      961 cgcacgtcat gcctatggtg atgtgtataa gaacacagag atgtatattg acggccccgg
     1021 agatgcttat ctggtatttg aaggtgcaga cggacagcag agaaaagagc tgatccatca
     1081 ttatgaggga ccgggtgtgc ttcagggtat gcacaatctg gatgattcta tcacaagttt
     1141 tgcaagatgc tgttttaatt atgcactgga tacaaaacag aacctgtggc ttggcggtaa
     1201 ggatacgatc tccaagattt atgacggacg ttttaaggaa atctttgcaa ctatttatga
     1261 agatgagttt aaggagaaat tcgaggcagc aggcattgaa tatttctaca gcctgatcga
     1321 tgatatcgta gctcgtgtca tgaaggctga aggcggattt atctgggcct gcaagaacta
     1381 tgatggagat gttatgagcg atatggtttc ttcagcattt ggttctctgg ctatgatgac
     1441 atctgtactg gtatctccac agggatatta cgaatatgaa gctgctcatg gaactgtaca
     1501 gagacattat tatcgctatc tgaaaggtga ggagacatct actaactctg tagcaactat
     1561 ttttgcatgg accggagctc tgagaaaaag aggtgagctg gacggcaata aagaattgat
     1621 ggaatttgct gataaactgg agaaggcgac tctggagacg attgaaagcg gtaagatgac
     1681 aaaagacctt gcgctgatca cttctcttga ggatgtgaca gtactgaaca gcaaagattt
     1741 catccttgcc atcagagaga gactggatga aattttataa tgtcagaaat tatccggtaa
     1801 tgtttctata acattataaa gttctccggg caggatcaca ctgcagcgga gaaacctgta
     1861 aaaagttata aactgcgaaa atgggaagaa acctgcatta tgcaagttct tcccattttt
     1921 catataatcc ttctaattct tcggtaatgg cggctttctc tttgctgagt ttcgcacatt
     1981 ctgccacatt tgtgcagata tccgggagaa tcatggtttc gtcgatctct ttatctctgg
     2041 tctcaagttc ttcaatgcgg gcttctactt tttttagttc attctctcgt tttcgctggc
     2101 gggcctgttc ttctttctgc tgctgccagg aaagcttgtt atcagaagcc tgctttgctt
     2161 ctgtcacagc ctgagcggca gccggagcat atttttccgt tagctcttct tttttctcca
     2221 gataatagtc gtaatctccg atataattta caattgcctg attggtcaga tccaggatcc
     2281 gggtagctgt ctggttgatg aagtaacggt cgtgggaaac atagaatact gttcctgtgt
     2341 aactgttcaa tgcctcttca agaatctctt ttgaagcgat atccagatgg ttggtgggct
     2401 catcgaggat cagaaagttt gcttcggaca gcatcagctt ggcgagggaa acacgacctc
     2461 tttctccgcc ggacagagcg gagatcagtt tgaatacatc atcccctgtg aaaaggaatg
     2521 ctgccagcat atttcgaatt tctgtttccg taagtgtggg ataagcatct gagatttcct
     2581 caaaaatagt tttctccata tgaagaacat ggtgttcctg gtcatagtag ccaatctgta
     2641 ccttggaacc aagagtaaat ctgccgctgt ctgcatcgat cagcccattg atgatcttta
     2701 acatggtggt tttgcctgtg ccgttattgc caatcagggc tacacgttct ccacgtttga
     2761 tctcaaagga gatgtcactg aaaagtgtct gtccgggaaa agatttggaa agactttcca
     2821 cagtaaggac atcatttccg ctgacaaatc ttggttccag ggaaatgcgc atctgattct
     2881 gaatctcctg tggtttatca atgcgctgga ctttatccag cattttctcg cggctttccg
     2941 cacgtttgat ggatttctcg cggttaaagg atttcagctt ggcaatgact gcttcctgat
     3001 gtttgatatc ccgctgctgg ttcagatagg ctttgtactg ggcgtcacgc agctgggctt
     3061 ttttcagggc ataggcagaa tagttgccac tgtacatacg catatttcct gcttcgattt
     3121 ccacaacttt tgttacgact ttgtcaagaa agtaacggtc gtgggatacg ataaatacag
     3181 cacccgggta attcagaaga taggtttcca gccaggaaat actctccata tccagatggt
     3241 tggtaggctc atccagaaga agaatgtcag ggctggcaag aagcagttta cccagagcta
     3301 cgcgggtttt ctggccgcct gaaagagttt ctatctggcg ttcgaaatct tcttctgtga
     3361 aaccaagacc ttttaataca cccatgatct cacttttgta agcgtaacca ttttccagtt
     3421 caaactggtg actcagtctt gtgtaagtat tcatcaggct gtccagagca tctccagtgg
     3481 tggttttcat ttcctgttcc agagagcgca gacggttctc catatcaata atgtgctgtt
     3541 tggtggaaat cagttcttcg taaatggtat gatggccatg aatgtcctga tactgggcaa
     3601 ggtagccgat atttttatct ttggcaagta ccacgtttcc tgaatccggg gagagctcat
     3661 gcataatgat tcgcagcaga gtcgtcttgc ctgcaccgtt attgccgatg agagctgcct
     3721 tttcgcggtc ttctatatgg aaactggcat ccttgagaat gattttttcg ccaaaggatt
     3781 tacagatatt ctgacatgat aaaatcatag gaattcctcc gtttgtaatt tgtccgcgta
     3841 ctattataat cgaaaatgcc tgtttttgaa agcattttaa gagattttgt ccagagaaaa
     3901 gaaagcaccg gatggatgca tggaagaaaa tatgatattc cggggaattt ttcagaatga
     3961 aattgataaa aaaatacaga aatatttggt caaaaagtac aaatttaatt tgacaataaa
     4021 ataacatctg ggtataattg acatagataa gctaaggagg gaaaatttgt ggaagctaag
     4081 gaaatttcga aagcggtaat caagcgtttg ccgcggtact accgctacct cggtgaattg
     4141 atggaggaga atgtagagcg tatatcttca aatgatctta gcaagaagat gcatgtgacg
     4201 gcttcccaga ttcgtcagga cttaaataat ttcggtggtt ttggtcagca gggttacggc
     4261 tataatgtac gctatttgta tacagaaatc ggtaagatcc ttgggctgga tactgtacat
     4321 ccgatgatta ttctgggtgc cggtaatctg ggacaggcat tggccaatta tgtggatttt
     4381 gaaaagcgcg gatttaagct ggttggtatt tttgacgtca atcctgtact tgaggggatt
     4441 gcagttcgtg gaatcgagat tcagatgttg agcgatcttc ctttgtttct gaaggagaat
     4501 gaggttgaga tcgcgatcct gactcttcct aaaaacaaag ccaaagagat ggcaaagatt
     4561 ttgattgata atggaattaa ggcaatctgg aattttgcac atattgatct ggatgccccg
     4621 gaggatgtaa tggtagagaa cgtacatctt tcagagagtc ttatgacttt gtcctataac
     4681 ctgagtgagt acagaaagga acatgaaggg atgtaaggga taactgtgaa acacaaggct
     4741 gtggtcatct gatcacagct tttctcttgt atgtaaatgt gttattgtaa acaggaggtg
     4801 aacagatgag agagattgta actgctgttg agatgaaaga actggatcat aatacgattc
     4861 agaaagccgg gatatcgtct ccggtactta tggagagggc ggcattaaag acggttgagg
     4921 agattgtaca gagattaaag aataaagaaa aagagaaaat ccttgtggtc tgcggaaccg
     4981 gcaacaatgg cggagacgga ttggcgattg ccagactgct tcagttgaga ggagtcagaa
     5041 cctggtatta tattgcgggt aaggaagaga aaatgactgc tgaaactgcc ggacagctta
     5101 aaacagcaga atattatcag gtgccccggg tgcataacct ggatccggat gaatatacta
     5161 ctatagtaga tgccatcttt ggggttggac tgagcagacc tgtagaggga aaatatgcag
     5221 agcttatcag tacaatgaac cgggcatctg cgtataaagt agctgtggat attccatccg
     5281 ggattgactc agataccgga tttgagaagg ggattgcatt ccgggcagat cttacggtta
     5341 cctttgcttt tcgcaaaaga gggctgtgtt tttatcccgg acgcatgtat ggcggtgaga
     5401 tcgttgttac ggatatcgga atctattcca tacccggaaa gaaaatctgt atgcatcatc
     5461 tggaacgtga agatttgaaa atgcttccgg agagagttcc ctatggaaat aaaggaacct
     5521 tcggtaaggt tcttcttata gctggcagcg aaggaatgtg cggagctgca tatctgagtg
     5581 cagcagctgc tctgaaaagc tctgcgggaa tggtgagaat tcagaccgtg gaggccaaca
     5641 ggattccgtt gcagacactg ttgccggaag ccatgatcac ctgtgatttt gatgagaaaa
     5701 aaaaccaggc aatgcttgac tggtgtgatg tgcttgtgat aggaccgggt cttggaactg
     5761 gagcgcagag cagggaaaga gctcagtggt tcctggcgaa ggcagctgag tgtaaaaaaa
     5821 cagtaattct ggatgcagat ggcctgaatc ttctgtcagt ccatgacaac tggaagtgtt
     5881 ttatcgggga acatgtgatc ctgacacctc atattggtga gatgagtcgt ctgtgcggaa
     5941 aagaaatcgg taagatccag gattgtctgg cacagactgc agctgattat gcaaaggagt
     6001 ctggagcggt ttgcgttctg aaggatgcct gtactgtggt ggcagatgct tttggagata
     6061 tgtatttgaa catttcgggt aatgcaggaa tggctacagc gggaagtgga gatgtgttaa
     6121 gtggtgtgct tgccggtata cagtgtatgt acctggcagc agaaaagaaa ttttctcctg
     6181 ttatgctggc tgcacttggt gtatatgtgc atgggctggc aggtgatctg gctgctgaaa
     6241 cagtagggca gcgagggatg accgcaggag atatcatctg ttttctgccg gaaatactaa
     6301 gatagaatat tcattttgag gcagtaaatc tgtgaaacaa tttgcagata ttaacatgaa
     6361 aaaataacag aggaaaatta ttatgaagaa agatagcaga gtaaaagcag tcatttcact
     6421 ggatgcagta gaacataatt tccgggaaat gagaaaaaat attgcagaag agacaaagat
     6481 gatcgcagtc ataaaggctg acgcttatgg acatggcgcg gtgccggttg cacatctcat
     6541 tgaggattat gattatatct ggggatttgc ggctgcaact gcggaagaag cgattcatct
     6601 gcgagaagcc gggattacaa aaccaatcct gatccttgga attgtttttg atgaatattt
     6661 tccggaactt gtacgttatg agatccgtcc tgcagtgtgc gaatatgagg aggcaaggaa
     6721 actttcagac gaagcggttc tgcagaaaaa aactgtacat attcatattg ctctggacac
     6781 aggaatgacc aggatcggtt ttgcggatgc acaggaaaat gtagaggaaa ttaaaaaaat
     6841 ttcagaactt ccaaatcttg agattgaggg tatgtttact cattttgcaa gagcagatga
     6901 gtatgacaga agtccggcta tggtacaatt ggagcggtat ctggattttt caagacgcgt
     6961 agaagaggca ggagtggaaa ttcctctgca tcactgttct aacagtgcgg gcatcatacg
     7021 tatgccggaa gcaaatctga atattgtccg tgccggaatc acaatttatg gaatttatcc
     7081 ttcttctgag gtggagagag atattgtaaa gcttgagccg gttatggaac ttaagagtca
     7141 tgtgacttat gtgaaggacg ttcctgccgg agcagcgatc agctacggcg gtacttatgt
     7201 ggctgacagg aagcttcgtg tggcaactgt tccggtgggc tatgcagacg gctattccag
     7261 gcagctttcc aataaaggct gggttctgat acatggacaa aaggctccga tccttggcag
     7321 agtctgtatg gaccagttta tggtagatgt gactgagatt ggtgatgtga agaagggcga
     7381 tgaggtaaca ctgatcggca gagacgggga tgagtttatc ggtattgagg agattggtga
     7441 tctctgtggc agattttcct atgagtttgc ctgtgatatc agcccgcgtg tgccgagagt
     7501 ctatattaaa aacgggaaag aaatataatg ttactgtttg ctgaatagag acgaagtgag
     7561 cgataactga tttttatata ccacgcatgt atacacaaga aaaaggtgga aatactttat
     7621 cttaaccgga caggaaatat ccggctgata acaaggtttc ttcttatggc aatgttgtaa
     7681 attgaaaccg gaaagaatcg gagtgtgaat tgtgtggtaa ttaaaagagg ggatatattt
     7741 tatgctgatc ttcgccctgt agtggggagt gaacagggtg gaatccggcc ggttctgatc
     7801 attcagaata acgtaggtaa ccggcacagc cctacagtga tctgtgctgc tattacatca
     7861 aaaatgaaca aggctaagct tcctacacat attgagatca atgcggaact ctatgggatt
     7921 gaaaaagatt ctgtgatttt gctggaacag cttcgtacta tagataaaag aagattaaaa
     7981 gataaagtct gtcatctgga tgatgcgatg ctggataagg tgaatcatgc acttgagatc
     8041 agtctggagc ttacgttctg attttacttt ttaagggata tatcatctga tccggtattg
     8101 cgattttttg gcttgcatgt ctcggatagt agtgcgtgaa caataaatga acagtaattt
     8161 ttggtatctc tgtccggtca gtatcttgac gattaatgga gaaagtgata tattttacca
     8221 gtaattacgc atattcaata aggaggagac tgaataatat aatggaagat ggcagtagta
     8281 tgcccctctg gggcttattt gttttactgt tactgttgtg gctgaatgga atcttttacg
     8341 ggtttgccgc agcgctgaga aacataagtg agaatgatac acagaagaaa gcagaggaag
     8401 gcgataaaaa agcacagatg ctgatgactc tgatcgacaa accggcgcag tttgtcaatg
     8461 taattccgct gatcgtaatg gcacgtggca tttgttttgg tacttttctg gtcccatatg
     8521 ctgtggatgc attttatcca tacataaagc atgtgcctgc actgatcctg gtaatggcat
     8581 tgtgcgtgat ttttcttgct gcaatcggta ttctggcttt tcggcgggtt ggtacttatc
     8641 atcctgaagc ttatgcatat aagtatctga acctggtaca tttctggctg aatctgttaa
     8701 agccttttac tgtatttgtc acctggatcg ccagactggc agcagtgcct tttggtgtgg
     8761 agataaaccg tacagaaaag tctgtcacag aagaggaaat catttccatt gtagatgaag
     8821 ctcatgaaca gggcgtcatc caggagaatg aggcggaaat gatccagaat atcatatctt
     8881 ttaatgagac agaagcccat gatatcatga ctcacagaaa gaatgtggtg gcctttgacg
     8941 aggagatatt gctcaagaac atgattgaca ctatgctgga agaggggaat tcccgttatc
     9001 cggtatatga ggagaatatt gacaatatca aaggaattgt ccattataaa gatgcgctta
     9061 agtttatgac acagaatccc tgggcgaagt tcaaaccgtt gaaggaactg cccggaatta
     9121 tccgccaggc atccctgatt cctgaaacaa gggggattgg agatcttttt catactatgc
     9181 aggccagaaa gatccatatg gcaattgttg tggatgaata tggccagaca gccgggatcg
     9241 ttaccatgga ggatattctg gaggagattg tcggagatat tctggatgaa tatgatgagg
     9301 atgaaattac gatccgcgca cagaaagata acagtctgat cattgacggg cttgcttatc
     9361 tggaggatgt gggagaagag ttggatgcag attttgggga tacagagttt gaaacactga
     9421 acggttatct gaccaatatt ctgggacata ttcctacaga taaggatgtt gatacaagca
     9481 taaaagcgat tggatactgt tttacgatct tgtctattgg taataaaacc attggcaagg
     9541 ttaaggtgga gcgggataat aatgttgctg tataaacagc agcagataaa aataattaac
     9601 aaaaggagaa agaaagatgt caggacattc aaaatttgca aacattaagc ataagaaaga
     9661 gaaaaacgat gcaaaaaaag gtaagatttt tacagtaatc ggacgtgagc tggttatggc
     9721 tgtcaaagca ggcggtccgg atccaaataa caacagcaaa cttcgtgatg taattgccaa
     9781 agcgaaagca aacaacatgc caaacgatac aatcgaacgt ggtatcaaaa aagcagcagg
     9841 tgctgattcc gcagacaact acgagaaagc tgtatatgaa ggatatggtc cgaacggtgt
     9901 tgctatcatt gttgagactc tgacagacaa caagaaccgt acagcaggta atgtacgtaa
     9961 tgcatttaca aagggtaacg gaagcatcgg aactcaggga tgtgtatcct atatgtttga
    10021 tcagaaaggt cagatcatta ttgacaaaga agagtgtgag atggatgcag acgagattat
    10081 gatgcttgct ctggatgccg gtgctgagga cttcagcgag gaagaggaca gttatgagat
    10141 tctcactgct ccggatgatt tcagtgcagt tcgcgaagct ctggaaaaag caggtctgcc
    10201 tattgcagaa gcacaggttg ccatgattcc tcagacttat gtggagctta cagatgagca
    10261 ggcactcaag gatcttcaga gaactctgga tcttctggat gatgacgatg atgtcacaga
    10321 ttactggaca aactgggatg agcagtaaaa taccgcaggt atattgagac tgcctgtttt
    10381 gggttattgc tgtcggacat gtatagttta taaaagaaac acacataata cctccataga
    10441 gaaaatggag gtatttttat gtctgaaaac agatatggag aaaaaagaga agagtctgaa
    10501 acagggatga agctgcaaaa ctgtgagaca caggagtcag aaaataaagt tcaggaaagt
    10561 accaatgagc agatcaaaga tatgggacag gtggttttaa acagcaattc ccgaaaacac
    10621 aaggtagagc ttctgaccat cattggtgag gtggaaggtc atgagtctgc accctcccat
    10681 tccaaaacta caaaatatga gcatgtactt ccaaagcttg ccatcattga ggatgatgaa
    10741 gaaatagaag gcttgctgat ccttttaaac acagtgggag gagatgtgga ggctgggctt
    10801 gccattgcag agatgattgc ctcattaagt ataccaacag tgtcactggt tctgggcggc
    10861 ggacattcta tcggagttcc gatggccgta tctgcggact attctttcgc agtaccaagt
    10921 gctacgatgg tgatccatcc ggtacgttcc aatggtatgt ttatcggagt ggctcagact
    10981 taccggaata tggagaaaat tcaggaccgt atcactacat ttatttccga acactccaga
    11041 gcatctcaga aacgtctgga agagctgatg cttgatactt ctcagctggt aaaagatgta
    11101 ggaaccatgc tggaaggaaa agaggcagta aaagaagggc tgatcgatga ggttggcggt
    11161 attaaggaag cgttggcaaa attatatgaa atgatagaaa aagagtgtta aaagtgaaaa
    11221 aaatcttata tatgctacaa atcaaccctt gccatatgtc agattttggc gtattattaa
    11281 tagaggttta ttatttgcga ggaggaaaac catgtataag attttaaaag ctgaaacact
    11341 ggcagctaag atccatctta tggttgtaca cgcaccgcgt gttgcaagcc attgtcagcc
    11401 aggacaattt attatcgtta agttagatga gaagggtgag agaatccctc ttactatctg
    11461 tgattatgat cgtgaagagg gtacggtcac aatcgtattc caggagattg gtgtatctac
    11521 catcaagatg gcagaattaa aagcaggaga tgcattccgc gatttcgtag gacctcttgg
    11581 atgtccttct gaatttgttg aggaagatat cgaagaatta aagaaaaaga aaatggtatt
    11641 tgttgccggc ggtgttggta cagcccctgt ttacccacag gtaaaatggc tccatgaaca
    11701 tggaatcaca gcagatgtta ttcttggtgc aaaaactaag gatatggtta tccttgagaa
    11761 agaactggga gaagtttcca atctttatgt tactacagat gacggttcct atggccgtgc
    11821 aggtatggtt accaagactc ttgaggatct ggtaacaaat gaaggcaaac attacgatgt
    11881 ctgtgtagcg atcggaccga tgatcatgat gaaatttgtc tgtcttctta ccaagaagct
    11941 tggcattcat acaattgtca gcatgaaccc gatcatggtt gacggaacag gtatgtgtgg
    12001 cgcatgccgc cttcaggttg gcgatgagat taagtttgca tgtgtagacg gaccggaatt
    12061 cgatggacat ctcgttgatt tcgaccaagc tatgaagaga agccagatgt ataagactga
    12121 agagggacgt gcattactga aacttcagga aggtgatacc catcatggcg gatgtggaca
    12181 ttgtggaggt gacaagtaat ggcagatgca aagatgttaa agaaagttcc tgtaagagaa
    12241 cagaatccaa aagtacgtgc aacaaacttt gaagaagttt gtcttggata caatcaggaa
    12301 gaagcaatgg aagaagcaca gagatgctta ggctgtaaga aaccgaaatg tgtggaaggc
    12361 tgtcctgtat ccattaatat tcctggattt attgaagaga tcaaagaggg aaagatcgaa
    12421 gaggcataca aagtgatcgg attatcctcc gcacttccgg ctatctgtgg acgtgtatgt
    12481 cctcaggaat cccagtgtga gggtaaatgt atccgcggag tcaaaggcga agctgtttct
    12541 atcggtaagc tggagagatt tgttgctgac tatgctctgg aacatgatat caaacctgtt
    12601 ggagcagaag ttaagaacgg acataaagtt gcagtgatcg gttccggtcc ttccggactt
    12661 acctgtgcag gtgatcttgc aaaagctggt tatgatgtaa ctgtatttga agctcttcat
    12721 gaacttggcg gtgttcttgt ttacggtatt ccggagttcc gtcttccgaa acagaaagtt
    12781 gttaagaaag aaattgaaaa agttaaagaa ttaggtgtta aattcgagac gaatgttgtt
    12841 atcggtaaat ccacaacaat cgatcagctg atcgaggacg aaggatttga agctgtattt
    12901 atcggatcag gtgccggact tccaatgttc atgggtatcc cgggtgagaa tgcaagcggt
    12961 gtattctctg ccaacgaata tcttacaaga agcaacctga tgaaagcttt tgatgattct
    13021 tatgatacac cgatcgcagc aggaaagaaa gtagcagttg taggcggtgg taatgttgct
    13081 atggatgctg ccagaacagc tttaagactt ggtgctgaag tacatatcgt atacagaaga
    13141 agtgaagcag agcttcctgc acgagcagag gaagttcatc acgcaaaaga agagggaatc
    13201 atctttgatc ttcttactaa cccgaaggaa atccttgtag atgagaacgg tcatgtaaaa
    13261 ggtatgaagg ttgtcaagat ggaacttggt gagccggatg cttccggaag acgtcgtccg
    13321 gttgaaattc cgggatctga atatgacatg gatgtagata cagttatcat gtctcttggt
    13381 acttccccga accctcttat ttcttctaca acaaaaggtc ttgaagtcaa taagagaaga
    13441 tgtatcattg ctgaagaaga aacaggtaag acatctaaag atggtgtata tgccggcggt
    13501 gatgctgtta caggtgcagc tactgttatc cttgccatgg gtgcaggtaa agctggtgcc
    13561 aaaggtatcg atgaatatct taaaaataaa taatgtaaat ggtacttgac agattttgac
    13621 gtctgtggca tgattactag tgttgataat catgaaaagc ttagaaggtg tttacaggga
    13681 tttattttcc gtcagagaga ggaagccatc ggctggaagc tgcctcaggt tcagatattt
    13741 cctgaatttc ccaccggagc cgcgtttctg aatgacagta tttactgagt aggaaatgac
    13801 gttactgcgc gttaagcaga ccgagagcct gtagaaaatg caggaagcag ggtggtaccg
    13861 cggatatttt actccgtccc tttagtggga cggagttttt tgtttgccaa ttctgctatt
    13921 ataacatgaa ttgcgagaac tgcttttgca acggagcgat ttgtggctat caagtcagag
    13981 tcagatttta cgttactgtt cattctgttc tcagtaacga tttttcttct ctgacattat
    14041 gacataaagg agaaagagaa catggatatg aaagaaagac ttcaggaact ggctgaagca
    14101 gccggaaaac ggatcagtga atccgaaggt ctggaaaaac tgaacgaagt acgtgtggca
    14161 taccttggta aaaaaggtga attgactgca attttaaaga gtatgaaaga tgttgcaccg
    14221 gaagagcgcc cgaaggttgg acagcttgta aacgaaacaa gaagcaagat agaagcactt
    14281 cttgatgaat ctaaacagaa actggaagaa gcagcacgtg aagagaagat gaaacaggaa
    14341 gtgattgatg tgactttacc tgcaaagaaa gctaaaatcg gtcaccgtca cccgaacacc
    14401 atcgccctgg aagaagtaga gcgtatcttt attggtatgg gttatgaagt agtagaggga
    14461 ccggaggtag agtatgcgga ttacaacttt actaaactga atattccgga aaatcatccg
    14521 gcacgtgatg aacaggatac tttctttatc aacgattcta tccttcttcg ttcccagact
    14581 tcaccggttc aggcacgtac tatggagaaa ggaaagctgc cgatccgtat gattgcacct
    14641 ggtcgtgtat tccgttcaga tgaagtagat gcaacacatt ctccttcttt ccatcagatc
    14701 gaaggtcttg ttattgacaa gaacatcact tttgcagatc ttaagggaac tttgcaggag
    14761 tttgcacagg aattattcgg acctgagaca aaaactaagt tcagaccaca tcacttcccg
    14821 ttcacagagc caagcgcaga ggtggatgta tcctgtttca agtgtggtgg taaaggctgc
    14881 cgtttctgta aaggttccgg ttggattgaa atccttggct gcggtatggt tcatccgaat
    14941 gtattaagca tgtgcggaat tgatccggaa gaatacacag gattcgcatt tggcgtaggt
    15001 cttgaacgta ttgcacttct gaaatatgag attgatgaca tgagactgct ctatgagaat
    15061 gatatccgtt tcctgaaaca gttctgatga aatgactgtc tgaaacaaca gagctatgca
    15121 gccaagagga aatttctgtc cgggaaagct gctataagtc tgagctataa tatagaaaga
    15181 ggattaacaa aatgaatact tcattatctt ggattaaaat gtatgtgccg gatcttgatg
    15241 tgacagcaca ggaatataca gatgccatga cactgacagg aacaaaagtg gaaggctttg
    15301 aaaaactgga cgcagatctg gataagatcg tgatcggtca gattgaaaaa attgagaaac
    15361 atccggatgc agataaactg atcatctgcc aggtgaatat cggaacagag actgtacaga
    15421 ttgttacagg cgctcctaat gtaaaagagg gagataaggt tcctgttgtt cttgacggtg
    15481 gacgtgttgc cggtggacat gacggaaaga aaacaccggg tggaattgaa attaaaaaag
    15541 gaaaacttcg cggaatcgat tcctatggta tgatgtgttc catcgaagag ctgggaagca
    15601 caagagaaat gtatccggaa gcaccggaat acggaatcta tatttttcct gaagacgctg
    15661 ttgtaggcga gagtgcgatc aaggcactgg gcctggatga tgttgtcttt gaatatgaaa
    15721 tcacatccaa tcgtgttgac tgctacggcg tactgggaat tgcaagagaa gcggcagcta
    15781 cgtttaatga gaaattctgt cctcctgttg tagagtgtaa gggaaatgat gaaaaagctt
    15841 ctgactatgt aaaagtaacg gtagaagatc cgaaactttg tccacgttac tgcgcaagag
    15901 tcgtaaagaa tgttaagatc ggaccatctc cgaaatggat gcagagatgt ctggcttcca
    15961 atggaatccg tccgatcaat aatctggtag atattacaaa ctatgtaatg gaagaatttg
    16021 gtcagccaat gcacgcatat gatctggaca ggattgcagg aaaagaaatc gttgtacgtc
    16081 gtgcagagaa tgatgagaaa tttgtaacgc tggatggaca ggaacgtacc atggatgatc
    16141 aggtactgat gatttgtgac ggtgagaaag cagttggtat tgccggtatc atgggtggag
    16201 agaattctat gatcacagac gatgtaaaaa ctgttctttt cgaggctgca tgttttgatg
    16261 gaacaaatat ccgcctttct tccaagagaa tcggtctgag aacagatgct tccggtaaat
    16321 tcgaaaaggg tcttgacccg aacaatgcgc aggcagctat cgacagagca tgccagctga
    16381 tggaggaact tggggcaggt gaagtcgtag gtggaatggt agatatctgc aatgaagtaa
    16441 gagaaccatc cagagttcct ttcaaaccgg agaagattaa tgctcttctt ggaactgacc
    16501 tgacaccgga agaaatgctt gcttatttgg caaaagtaga actgacctat gacgaaaaaa
    16561 caaatgagat cgtagctcct actttccgtc aggatatcca ttatgtagct gatgttgcag
    16621 aagaagttgc aagatttttc ggatatgata agattcctac tactctgcct acaggtgaag
    16681 ctacaacagg taagcttccg tttaaacttc gtattgaagc agtggcacgt gatattgcgg
    16741 aatattgtgg tttctctgaa ggaatgacct attctttcga aagtccgaag gtatttgaca
    16801 aattaagact tccggctgac agtaagctcc gtcagggaat cgtgatcagc aatcctctgg
    16861 gagaagacta cagtgtcatg agaacaacta ctttaaacgg tatgctcagt tctctggcta
    16921 cgaact
//
LOCUS       DBMDXG010000007        15920 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1100_length_15920_cov_1.926102, whole genome shotgun sequence.
ACCESSION   DBMDXG010000007 DBMDXG010000000
VERSION     DBMDXG010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 15920)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15920)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15920
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1100_length_15920_cov_1.926102"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="ACZPD3_00595"
     CDS             <1..1026
                     /locus_tag="ACZPD3_00595"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HIR1414552.1"
                     /translation="VSPKLIQKAKEQSIVIIQTPYDTFTIARLINQSIPVRYIMKTDN
                     LVTFNTEDFTDDIQNEMIKHRHRAFPVINKKGKCIGTISRRNFLDMHRKKVALVDHNE
                     KDQAVDNIDKAEIVEIIDHHKLGSLETMVPISFRNQPVGCTATILYEMYGEQKLEISP
                     SIAGLLCAAIISDTLMFRSPTCTLSDKMAAGALALIAGINIEQFAKEMFKAGSNLKDK
                     SPEEIFYQDYKKFIAEDEINFGVGQISSMDSDELAEIKERLVPFMVSECGRHGVTRVF
                     FMLTNIIEESTELLYYGEGSEEMARIAFHMEPKDGVFDLKGVVSRKKQLIPALMEAAQ
                     AGQNDYN"
     gene            1061..1651
                     /locus_tag="ACZPD3_00600"
     CDS             1061..1651
                     /locus_tag="ACZPD3_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR1414553.1"
                     /translation="MEKETWKAGNMLYPLPAVMVSVSDGEGNDNIITVAWAGTVCTNP
                     PMVSISVRPSRFSYDMLRKTGEFVINLTTEKLAYATDYCGVRSGRDVDKFKEMKLTKE
                     KADFVKVPMIAESPVSIECKVRQVLELGSHHMFLADVLAVHADPQYMDEKKKFHLNDA
                     KPLVYSHGEYLGIGKKLGTFGYSVKKKKKKSGKKKQ"
     gene            1703..2215
                     /locus_tag="ACZPD3_00605"
     CDS             1703..2215
                     /locus_tag="ACZPD3_00605"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1414554.1"
                     /translation="MNNVTLIGMPGSGKSTIGVILAKALRYEFLDSDLLIQKQEKRKL
                     SEIIEQDGPEKFKEIENQVNADIHVTDTVIAPGGSVIYCDEAMEHLKSIGKVIYLKLS
                     LESLSKRLGNLKGRGVLLKDGQSLKDLYEERVPLYEKYADITIDEEGKDLDESLRAIL
                     EIIHIKEQGK"
     gene            2432..3724
                     /locus_tag="ACZPD3_00610"
     CDS             2432..3724
                     /locus_tag="ACZPD3_00610"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1414555.1"
                     /translation="MEQYVIKGGNPLVGEVEIAGAKNAALAILAAAIMTDETILIENL
                     PDVRDINVLLEAIAGIGAQVDRIDKSTVKINGSTIGDVSVDYEYIKKIRASYYLLGAL
                     LGKYKHAEVPLPGGCNIGSRPIDQHLKGFRALGADVDIMHGAIVAKADELHGSHIFLD
                     VVSVGATINIMMAASLAPGRTILENAAREPHVVDVANFLNSMGANIKGAGTDVIRIKG
                     VEKLHRTEYSIIPDQIEAGTFMFAAAATGGDVTVKNVIPKHLEATTAKLEEIGCEVEE
                     FDDAVRVRAPKRLHRTHVKTLPYPGYPTDMQPQIAVTLALAEGTSIVTESIFENRFKY
                     ADELSRMGANIKVEGNSAIIDGVKKLTGARVSAPDLRAGAALVIAGLAADGITVVDDI
                     VYIQRGYENFEDKLRSLGAEIERVSNEKEIQKFRLRVG"
     regulatory      3823..3924
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4087..5268
                     /gene="metK"
                     /locus_tag="ACZPD3_00615"
     CDS             4087..5268
                     /gene="metK"
                     /locus_tag="ACZPD3_00615"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR1414556.1"
                     /translation="MEKILFTSESVTEGHPDKMCDAISDAILDALMEKDPMSRVACET
                     ATTTGLVLVMGEITTNAYVDIQKIVRDTIREIGYTRGKYGFDADTCAVITAIDEQSAD
                     IALGVDKALEAKMGEDEIDAIGAGDQGIQFGYASNETEEYMPYAINMAHKLARQLTKI
                     RKDGTLKYLRPDGKTQVTVEYDEAGKPIRIDAVVCSTQHDPDVTQEQIHEDIKKYVFD
                     EIIPADMVDENTKYFINPTGRFVIGGPHGDSGLTGRKIIVDTYGGTGRHGGGAFSGKD
                     CTKVDRSAAYAARYVAKNIVAAGLADKCEIQLSYAIGVAHPTSIHVETFGTGKLSDTK
                     LVEIVRENFDLRPAGIIKMLDLRRPIYRQTAAYGHFGRTDVDLPWEHLDKVDDLKKYL
                     A"
     gene            5506..6648
                     /locus_tag="ACZPD3_00620"
     CDS             5506..6648
                     /locus_tag="ACZPD3_00620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414557.1"
                     /translation="MKLKTRIIVGFGMSILVPLLLFSATLYGLGHTKFAQQTKDSSEV
                     VYDISVGESTHSQTQVRIIAKDLLFTATVILVFTALSIGLWIYRSIATPLVKLKKATQ
                     NIKEGNLDFVLEVDGDDEFSELCRDFEEMRKRLKESAEEKVLMDKENKELISNISHDL
                     KTPITAVKGYVEGIMDGVADTPEKIDRYVKTIYNKTNEMDHLINELTFYSKIDTNRIP
                     YTFSKLNVEDYFSDCAEEVGLELETRGIELVYANYVEDNVQVIADGEQIRRVIHNIIS
                     NAIKYMDKPKGIIQIRIKDVGDFIQVEIEDNGKGIAAKDLPSIFDRFYRTDVSRNSSK
                     GGSGIGLSIVKKILEDHGGKVWATSRLGIGTIMYFVLRKYQEVPMK"
     gene            6645..7334
                     /locus_tag="ACZPD3_00625"
     CDS             6645..7334
                     /locus_tag="ACZPD3_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1414558.1"
                     /translation="MSRILIIEDEEAIADLEKDYLELSGFEVEICNRGDTGLTRALNE
                     EFDLIILDLMLPEVDGFDICRQVRQEKNTPIIMVSAKKDDIDKIRGLGLGADDYMTKP
                     FSPSELVARVKAHMDRYNRLIGSNVRKNDIVEIRGIKIDKTARRVWVNGEEKTFTSKE
                     YDLLTFLAENPNRVFTKEELFREIWDMESVGDIATVTVHIKKIREKIEFNTAKPQYIE
                     TIWGVGYRFKV"
     gene            7452..8918
                     /locus_tag="ACZPD3_00630"
     CDS             7452..8918
                     /locus_tag="ACZPD3_00630"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1414559.1"
                     /translation="MERLYKKLESYGQSDYYPFHMPGHKRNRASSADDFLFERDITEI
                     SGFDNLHHAEGILKEAQEYAAQIYGTKKCFFSVNGSTAALLAAVSASVNKGGKILVAR
                     NCHKAVYHALYLRELQPVYIYPHEDQRLGINGGISPERVERYLEENTDVQAFLLTSPT
                     YDGVVSDIKTIAEVVHRHKIPLIVDEAHGAHFHFSKYFPVSAADLGADIVIQSFHKTL
                     PSMTQTAVLHICSDMADVEKIKRFMGIYQTSSPSYILMASMDACMDKLRKDGQQMFRE
                     FTFNLEKARQRLSKCEKIKLIEGSMIEGSGIYDFDRSKLLFSTVGTSVNGRLLHQILR
                     DRYHIEMEMAAEKYVLGIAAVGDTEEGFERLCTAIEEIDAEIQQTDESEESQYHTSHA
                     RMTQLMTISQAVDAQQRRYSLKESVGKVSAEFAYLYPPGIPIIVPGEQITGQFVRNVR
                     RYMEQGLEVQGLSDTSAETICVAARNEIGQEEYSPAKE"
     gene            8929..9516
                     /locus_tag="ACZPD3_00635"
     CDS             8929..9516
                     /locus_tag="ACZPD3_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR1414560.1"
                     /translation="MGKIFYIMGKSASGKDRIYSLLAAHKELNLKTLILYTTRPIRVG
                     EQDGKNYYFVDDGKLEEFRKNGNLIEERAYHTVYGIWTYFTADDGQVNLADSDYLGIG
                     TLESFKKMRKYYGEDAVCPVYVQVEDGERLSRALNREREQENPRYEEMCRRFIADQSD
                     FSEENILNAGIEKRFQNINLDDCVKEIANYIKSVQ"
     gene            9569..10558
                     /gene="holB"
                     /locus_tag="ACZPD3_00640"
     CDS             9569..10558
                     /gene="holB"
                     /locus_tag="ACZPD3_00640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR1414561.1"
                     /translation="MMGFNNIIGHEEIIGHLKNAIESGKISHSYIFTGEPGSGKKLLA
                     GTFASTLQCEAGGTEPCQKCDSCKKAMGKNHPDIIMVSHEKPGTITIDEIRDQVINDI
                     DIRPYYSPYKIYIIADADLMTPQAQNALLKTIEEPPEYAVILLLTNNIGGLLPTIQSR
                     CVRLDLKVVDDGLVKKYLMEHLHVPDYQAEIDASFAQGSIGRAKEAATSQEFAEMTQN
                     ALRILKYANTMEVYELSDAIKSLSEDKQNINDYLDIFQFWFRDVLMFKATQEIDNLVF
                     KQEINFIREQAKQRSYENLENILDSIQKTKVRLKANVNFDLAFELLFLTIREK"
     gene            10560..11459
                     /locus_tag="ACZPD3_00645"
     CDS             10560..11459
                     /locus_tag="ACZPD3_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR1414562.1"
                     /translation="MTKVVGVRFRNVGKIYYFSPKDYEIKTGDHVIVETARGIEYGKV
                     VLAPREVDEEDVVHPLKEVLRVATKEDDEREAQNRVREREAFKICQKKIREHGLEMKL
                     IDAEYTFDNNKVLFYFTADGRIDFRQLVKDLAAIFKTRIELRQIGVRDETKILGGIGI
                     CGRCLCCHTYLSEFAPVSIKMAKEQNLSLNQTKISGVCGRLMCCLKNEQETYEELNKK
                     LPGLGDTVTTPDGLTGTVHSVNVLRQRVKVIVEINDEKEIQEFPVDDLKFRPRKKKVK
                     VSEKELKELGNLEDKKGDSKLND"
     gene            11452..12192
                     /locus_tag="ACZPD3_00650"
     CDS             11452..12192
                     /locus_tag="ACZPD3_00650"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR1414563.1"
                     /translation="MTDQLVKDNERIDDLQNGYYVIQDPDKFCFGMDAVLLSGFAKVK
                     KGETALDLGTGTGIIPILLKTKTNGKHFTGLEIQKECADMAGRSVRYNHLEDDVEIVR
                     GDIKEAADIFGAASFDVVTSNPPYMIGQHGLRNSDMPKAIARHEVLCNLEDVVSQASK
                     VLKERGRFYMVHRPFRLAEIMNFLTKYRLEPKRMQLVYPYIDREPNMVLIEALKGGNS
                     RVTVEPPLIVYKEPGVYTENILKIYDMI"
     gene            12233..13084
                     /gene="rsmI"
                     /locus_tag="ACZPD3_00655"
     CDS             12233..13084
                     /gene="rsmI"
                     /locus_tag="ACZPD3_00655"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR1414564.1"
                     /translation="MSGKLYLCATPIGNLEDITLRVLRTLKEVDLIAAEDTRNSIKLL
                     NHFDIKTPMTSYHEYNKIDKAYVLISKMQEGQNIALITDAGTPGISDPGEELAAMCYE
                     AGIEVTSLPGPAACITALTLSGLPTRRFAFEAFLPADKKERKLILEELKNETRTIILY
                     EAPHRLIRTLEELKETLGNRRMTLCRELTKRHETAFHTTIEELILYYQTEKPLGECVL
                     VIEGRSRQEMEEEQKASWEKITIEEHMEIYENQGHSRKEAMKMVANDRGMTKRDVYQY
                     LINKTEE"
     gene            13282..>14064
                     /locus_tag="ACZPD3_00660"
     CDS             13282..>14064
                     /locus_tag="ACZPD3_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1414565.1"
                     /translation="MGGENTEYGADQIQILEGLEAVRKRPGMYIGSTSGRGLHHLVYE
                     IVDNAVDEALAGFCTEIQVVINPDNSITVVDNGRGIPVGINHKAGIPAVEVVFTILHA
                     GGKFGGGGYKVSGGLHGVGASVVNALSTWLEVRIYHEGKIYQQRYERGKTMYPLKVVG
                     DCQPDKTGTTVVFLPDKEIFEETVYDYDILKQRLREMAFLTKGLKISLKDTREGMERE
                     KVFHYEGGIREFVSYLNRSKEALYPEIIYCEGQQNGVSVEVAM"
     assembly_gap    14065..14074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14075..15209
                     /locus_tag="ACZPD3_00665"
     CDS             <14075..15209
                     /locus_tag="ACZPD3_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR1414566.1"
                     /translation="DSYTENCYGFVNNINTPEGGTHIVGFRNALTKTFNDYARKNKLL
                     KDSEPNLSGDDIREGLTAIVSVKIENPQFEGQTKQKLGNSEARGAVDSVVSKQLEIFL
                     EQNPTVAKTTVEKSVLAQRAREAARKARDLTRRKSALDNMSLPGKLADCSDKNPENCE
                     IYIVEGDSAGGSAKTARNRVTQAILPLRGKILNVEKARLDKIYANAEIKAMITAFGTG
                     IHEDFDISKLRYHKIIIMTDADVDGAHIATLLLTFLYRFMPELIKQGYVYLAQPPLYK
                     VEKNKKVWYAYDDDELNQILTEIGRDGNNKIQRYKGLGEMDADQLWETTMDPEKRILL
                     KVTMDEAATSEIDLTFTTLMGDKVEPRREFIEENARFVKNLDI"
     gene            15229..>15920
                     /locus_tag="ACZPD3_00670"
     CDS             15229..>15920
                     /locus_tag="ACZPD3_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR1414567.1"
                     /translation="MEDNIFDKVHEVDLEKTMKDSYIDYAMSVIASRALPDVRDGLKP
                     VQRRVLYSMIELNNGPDKPHRKCARIVGDTMGKYHPHGDSSIYGALVNMAQEWSTRYP
                     LVDGHGNFGSVDGDGAAAMRYTEARLSKISMEMLADINKDTVDFQPNFDETEREPVVL
                     PSRYPNLLVNGTSGIAVGMATNIPPHNLREVVDAVVKIIDNIIEEQRETTMEEILDIV
                     KGPDFPTGASGN"
BASE COUNT         5192 a         2640 c         3945 g         4133 t
ORIGIN      
        1 gtttctccga aattgattca gaaggcaaaa gaacagagta tagtgatcat tcagactccg
       61 tatgatacat ttacgatcgc aagactgatc aaccagagta ttcctgtacg gtatattatg
      121 aaaacagata atctggttac ttttaataca gaggatttta cggatgatat tcagaatgag
      181 atgatcaaac accgtcatcg tgcgttccct gtaattaaca aaaagggaaa atgtatcgga
      241 acaatttccc gaagaaattt cctggatatg cacaggaaaa aagtagcact tgtggaccat
      301 aatgagaaag atcaggcagt tgacaatatc gataaagctg aaatcgtgga gattattgac
      361 catcataaac tgggttctct ggaaactatg gtaccgattt ctttcagaaa tcagccggtc
      421 ggatgtacag caacgattct gtatgaaatg tatggggagc agaaacttga gatttcaccg
      481 agtattgcgg ggcttctctg tgctgcgatt atttcagata cactgatgtt ccgttcacct
      541 acctgcacat tatctgacaa gatggctgca ggtgcgctgg cacttattgc aggaatcaat
      601 attgagcagt ttgcaaaaga gatgtttaaa gcaggaagta acctgaaaga caaatcaccg
      661 gaagaaattt tctatcagga ttataagaaa tttattgctg aggatgagat caactttggt
      721 gttggacaga tcagttccat ggacagcgat gagctggcag aaatcaaaga gcgtctggtt
      781 ccgtttatgg tcagcgagtg tggccgtcat ggtgttaccc gcgtattctt tatgcttacc
      841 aatattattg aggaatctac agagcttctc tattatggtg agggaagtga ggaaatggca
      901 cgtattgctt tccatatgga gccaaaggat ggtgtatttg atctgaaagg cgttgtttca
      961 cgtaagaaac agctgattcc ggcacttatg gaagctgcac aggccggaca gaacgattac
     1021 aactgatggc agcagacaga atagctgagg ataataagaa atggaaaaag aaacctggaa
     1081 agcaggaaat atgctttatc cacttccggc agttatggta agtgtttctg acggagaggg
     1141 gaatgacaat attattacag ttgcatgggc aggaactgtc tgcacgaatc cgccgatggt
     1201 gtctatttct gtgcgaccat cccgtttttc ctatgatatg ctcagaaaga cgggagaatt
     1261 tgtaataaat ctgacaacag agaaactggc atatgcaacg gattactgtg gagtccgttc
     1321 cggcagggat gttgataaat ttaaagaaat gaagcttacc aaagagaagg cggattttgt
     1381 gaaagttccc atgatcgcag aatctccggt gagtattgaa tgcaaagtaa ggcaggttct
     1441 ggaattgggt tcccaccata tgtttctggc ggatgtgctt gcagttcatg ctgatccgca
     1501 gtatatggac gagaagaaga aatttcatct gaatgatgcg aaacctctgg tgtattctca
     1561 tggtgaatat ctgggaattg gtaaaaaact gggaacattt ggatacagcg ttaaaaaaaa
     1621 gaagaaaaaa tccggaaaaa agaaacagta atccgtaatt tgcagacgca gacaatgtat
     1681 gataaaatga aaggacataa acatgaataa tgtgacatta ataggaatgc ccggttcagg
     1741 gaaaagtaca ataggagtta ttcttgcaaa agcattgaga tatgaatttc tggattcgga
     1801 tcttctgata cagaaacagg agaaaagaaa gctttcagag atcatagagc aggacggacc
     1861 ggaaaaattt aaggaaattg aaaatcaggt taatgcagac attcatgtga cagatacggt
     1921 cattgcaccc ggcggaagtg ttatttactg tgatgaggca atggaacatc tgaagtctat
     1981 aggtaaggtt atatatctta aattatcact ggaatcttta tccaaaagac tgggaaatct
     2041 gaaaggccgc ggagttctgt taaaggacgg tcagagcctg aaagatctgt atgaggaacg
     2101 tgtacctctg tacgagaaat atgcagatat taccattgat gaagagggga aagatttgga
     2161 tgaaagtctt cgggcaattc tggagataat acatatcaaa gagcagggaa agtgattatt
     2221 acagctcggg aaaaggacag tggtaaataa tttttgttta caaatgataa atatatgata
     2281 taatatacaa ctgactttaa aataggatga tcgtttagta taaacatata tgcatttgaa
     2341 acagaagtta catgttcgca gaaaacgaaa aacagagtga acagtgacca ttaattctgc
     2401 tcatggagtg aaaataaata agaggtgtga tatggaacag tatgttataa aaggaggcaa
     2461 ccctttagtc ggtgaagttg agattgccgg tgccaagaat gcggcacttg ctattcttgc
     2521 agcagccata atgactgatg agacgattct gatagagaat ttgccggatg taagggatat
     2581 caatgtattg cttgaagcga tcgctggtat tggagcacag gtagaccgta ttgataaatc
     2641 tactgtgaag attaacggat ctacaattgg agatgtaagt gttgattatg aatatatcaa
     2701 gaagatcaga gcttcctact atctgctggg agcacttctt ggtaagtata aacatgcaga
     2761 ggtaccgctt ccaggtggat gcaacatcgg aagccgtccg atcgaccagc atctgaaggg
     2821 attcagagcg cttggtgcag atgtagatat catgcatgga gcaattgttg cgaaagcaga
     2881 tgaacttcat ggaagccata ttttccttga cgttgtctct gtaggtgcaa cgatcaatat
     2941 tatgatggca gcctcattgg ctccgggacg tactattttg gaaaatgccg caagagaacc
     3001 gcatgtagtt gacgttgcca acttcctgaa cagtatgggg gcaaatatta aaggtgcagg
     3061 tacagatgtg atccgtatta aaggtgtgga gaagctccac cgtacagagt attctattat
     3121 tccggatcag atcgaggcag gtacatttat gtttgctgcg gcagcaacag gcggagatgt
     3181 aacagttaag aatgtaattc cgaagcatct ggaagcaact acggctaaac ttgaagaaat
     3241 tggatgtgag gttgaggaat ttgatgatgc agttcgtgta cgtgcaccaa agagacttca
     3301 ccgtactcat gtaaagactc ttccatatcc cggatatcct acagatatgc agccacagat
     3361 tgctgtaact ctggcacttg cagagggaac cagtattgta acagagagta tttttgagaa
     3421 cagattcaag tatgcggacg aactttcacg tatgggagca aacatcaaag ttgagggaaa
     3481 ttcagcaatt attgatggtg tgaagaagct tacaggtgcg agagtaagtg caccggatct
     3541 gcgtgcaggt gcagccctgg tgatcgcagg tcttgcagca gatggaatca cagttgttga
     3601 tgatatcgta tatatccaga gaggatacga gaattttgaa gataagctga gatctctcgg
     3661 tgctgagatt gagagagtat ccaatgagaa agaaatccag aaattccgtc tcagggtggg
     3721 atgataaaaa ataccaaaaa attacttgac atttgtaagg gttaagtata ttgtatgata
     3781 tgcagcaatc ttaattaatg caagttaata attagtaata gccttttatt cagagtggtg
     3841 gagatacaaa ggatctgcga agccacggca acccctgttt gtgtaggaag gtgccaacct
     3901 gagcgagtga tcgaacaata agaggattgt tgagataatc agacagtccg attacttcgg
     3961 gctgtttctt tttgttaacg gaaaagtatc agcggagaga aataatctgt tgatacagta
     4021 cataaatggc tgtgggttat tctgcggtcg ggaaacaatt gtaaatgaat aaaaaaggag
     4081 aacacaatgg agaaaatttt atttacttct gaatctgtaa ctgaaggcca tccggataaa
     4141 atgtgtgatg ctatttccga tgctattctt gacgctctta tggaaaaaga tcctatgagt
     4201 cgtgttgcat gtgaaactgc tacaactaca ggtcttgtac ttgttatggg tgagattaca
     4261 acaaatgcat atgttgatat ccagaaaatc gtaagagata ctatccgcga aatcggatat
     4321 acacgtggaa aatatggatt tgatgctgat acatgtgcag ttatcacagc aatcgatgaa
     4381 cagtctgctg atatcgctct tggcgtagat aaagctcttg aagcaaaaat gggtgaagat
     4441 gaaattgatg ctatcggagc aggtgaccag ggtatccagt ttggttatgc ttccaatgag
     4501 acagaagagt atatgccata tgccatcaac atggcacaca aacttgcacg tcagttaacg
     4561 aagatccgta aagacggtac attaaaatat ttaagaccgg acggaaaaac acaggttact
     4621 gtagaatatg atgaagccgg aaaaccaatc cgtatcgatg cagtagtatg ctctacacag
     4681 catgatccgg atgtaactca ggagcagatc catgaggaca ttaagaaata cgttttcgat
     4741 gagatcattc cggcagatat ggtagatgag aacacaaaat atttcatcaa cccaacagga
     4801 cgtttcgtga tcggcggacc tcatggtgac agcggactta ccggacgtaa gatcatcgtt
     4861 gatacttacg gtggaaccgg acgtcatggc ggcggtgctt tctccggtaa agactgtaca
     4921 aaggtagacc gttccgcagc gtatgctgca cgttatgttg caaaaaatat tgttgcagca
     4981 ggtcttgcag ataaatgtga gattcagctt tcctatgcga tcggtgttgc tcatccgaca
     5041 tctatccacg tagaaacatt cggaaccggt aaactttctg atacaaagct ggttgagatc
     5101 gttcgtgaga actttgacct tcgcccggcg ggaattatca agatgcttga tctgagaaga
     5161 ccgatttaca gacagacagc tgcttatgga catttcggac gtacggatgt agaccttcca
     5221 tgggagcatc tggacaaagt tgatgacctt aagaaatacc ttgcttaaat aagaaaatcg
     5281 taactgttca gtacttgcac agacagtgga atgctgaata attacagaat atcacaaaaa
     5341 ttgcagtgca atgggataaa acaggaatta tctgaaagaa caggacagat gattcctgtt
     5401 ctttttatag aaaatttaga tatgtaaaac agactttatg atataatgct tgcagagggc
     5461 aaaattatag aaaccttata aatcaacaga taaaggatga tcgggatgaa gttaaaaaca
     5521 aggattatag ttggattcgg tatgagtatt ctggttcctc tgcttttatt ttcggcaact
     5581 ttgtacggat taggtcacac aaagtttgca cagcagacaa aggattcttc ggaagtggtg
     5641 tatgatattt cagtagggga atccacccac agccagacac aggtaaggat tattgcaaaa
     5701 gatctgctgt ttactgcgac ggtaatatta gtattcacag ctttatctat tggattatgg
     5761 atttacagaa gtattgccac accgctggtt aaattaaaga aagccactca gaatatcaaa
     5821 gagggaaatc ttgattttgt tctggaagta gatggtgacg atgagttttc ggaactttgc
     5881 cgggattttg aggagatgag aaagcggctg aaggaatcag cagaagagaa agttcttatg
     5941 gataaagaaa ataaagaact tatcagcaat atttctcatg atcttaagac gccgatcaca
     6001 gcggtaaagg gatatgtaga ggggattatg gacggcgtag cggatacgcc ggagaaaata
     6061 gaccgttatg tgaagactat ttataataag acgaatgaga tggatcatct gatcaatgag
     6121 ctgacttttt attcaaagat tgataccaac agaattccat atactttcag taaactgaat
     6181 gtagaagatt atttcagtga ttgcgcagaa gaagtaggac tggaactgga aacgagagga
     6241 attgaactgg tatatgccaa ttatgttgag gataatgtgc aggtgatcgc agatggagaa
     6301 cagattcggc gtgttattca taacattatc agcaatgcta tcaaatatat ggataaaccc
     6361 aaaggtatca tccagattcg cataaaggat gtgggagatt ttattcaggt tgaaattgaa
     6421 gataatggaa agggaattgc agcaaaggat ctgccgtcta tttttgatcg tttttacaga
     6481 acagacgtgt cgagaaattc atccaaggga ggaagcggaa tcggtctttc cattgtaaaa
     6541 aagatcctgg aggatcatgg aggaaaggtc tgggcaacca gcaggcttgg aatcggcaca
     6601 atcatgtatt ttgtacttag aaagtatcag gaggtaccaa tgaaatgagc agaattttaa
     6661 ttatagaaga tgaagaggca attgcagatc tggagaagga ttatctggag ctttccggat
     6721 ttgaagttga aatctgcaac agaggagaca ccggattgac gcgtgcgctg aatgaggagt
     6781 ttgacctgat tattctggat ctgatgcttc cggaagtgga tggctttgat atctgcaggc
     6841 aggtaagaca ggaaaaaaat actcctatca tcatggtatc tgcaaagaaa gatgacatag
     6901 ataaaatcag aggtctggga ctgggggcag atgattacat gacaaaacca ttcagtccaa
     6961 gtgaactggt agccagagtc aaggcacata tggacagata taatcgtctg atcggttcca
     7021 atgtacgcaa aaatgatatt gtggagatcc gcgggatcaa gattgataag actgcacgcc
     7081 gcgtatgggt aaacggagag gagaaaacat ttacttccaa ggaatatgat ctgcttactt
     7141 tccttgcaga gaatccgaac agagtgttta ccaaggaaga gttgttccgg gaaatctggg
     7201 atatggaatc tgtgggagat attgcaacag tcactgtcca tatcaagaag atccgggaga
     7261 aaattgaatt taatactgca aaacctcagt atattgaaac aatctggggc gtaggataca
     7321 gatttaaagt ttaactgaat cagggaagtt ccatatttca ggcacgaaaa ggcaataatc
     7381 agtgggaaat ttggaacgca agattgttcg agaaatcaac ttgttggcac aatagtataa
     7441 aaaggaaaaa aatggaaaga ttatataaaa aattagaaag ctatgggcag tctgactatt
     7501 atccgtttca catgccgggg cataagagaa acagggcatc ttcagcagat gactttcttt
     7561 ttgagagaga tattacagaa atttcaggat ttgacaatct tcatcatgcg gaagggattt
     7621 tgaaagaagc acaggaatat gcagctcaga tttatgggac aaagaaatgt ttcttcagtg
     7681 taaatggaag tacggcggct cttctggcag cagtcagtgc aagtgtaaat aaaggtggga
     7741 agatacttgt cgcgaggaac tgtcacaagg ctgtttacca tgcattgtat ttacgtgagc
     7801 ttcagccggt ctatatttat ccacatgaag atcagagact ggggataaac ggaggaattt
     7861 ctcctgaacg tgtggaaaga tatctggaag aaaatacgga tgtgcaggct tttctgctta
     7921 cttcgcctac atatgacggc gttgtgtctg atataaagac aattgcagaa gttgtacaca
     7981 gacataagat tcctcttatt gtggatgaag cacatggtgc acattttcat ttttctaagt
     8041 attttccggt gtctgctgct gatctgggag ctgacatcgt aatacagagt ttccataaaa
     8101 cgctgccatc tatgactcag acggcggtgc ttcatatatg cagtgatatg gcagatgtgg
     8161 aaaagataaa gagatttatg gggatttacc agaccagcag tccgtcgtat attctgatgg
     8221 cgagtatgga tgcatgtatg gataaactga gaaaagacgg gcagcaaatg tttcgtgaat
     8281 ttaccttcaa tctggagaag gccagacagc gtctttcaaa gtgtgaaaaa ataaaactga
     8341 tcgaaggttc tatgatcgaa ggcagtggaa tctatgattt tgacagatcc aagctgttgt
     8401 tctccacagt cggaacttct gttaacggac gccttctcca tcagatactt cgtgacagat
     8461 atcatattga aatggaaatg gcggctgaga aatatgttct tggaattgca gcagtgggag
     8521 atacagaaga gggatttgag agactgtgta ctgcaattga agagattgat gcagagatac
     8581 agcaaacaga tgagtcggag gaatcgcagt atcatacttc tcatgcgaga atgacacagc
     8641 ttatgacaat ttctcaggca gtggatgcgc agcaaaggcg atattctctg aaagaaagtg
     8701 ttggcaaagt cagtgcagag tttgcttatc tgtatccgcc cggaattccg attattgtgc
     8761 cgggagagca gataactgga caatttgtaa gaaatgtgag aagatacatg gaacaggggc
     8821 tggaagtaca gggactcagt gatacatctg ctgaaacaat ctgcgtagct gcccgaaatg
     8881 aaattggaca ggaagaatac agtccggcaa aggaataaca taacagatat gggaaagatt
     8941 ttttacatca tgggaaagag tgcatccgga aaagacagaa tctattcact tctggccgca
     9001 cataaagaat tgaatctgaa aacacttatt ctgtatacaa cccgcccgat cagggtagga
     9061 gagcaggatg gaaaaaatta ttattttgta gatgacggaa aactggaaga attcagaaaa
     9121 aacggaaatc ttatcgagga gagagcatat catacggttt atgggatatg gacttatttc
     9181 actgcagatg atggtcaggt gaaccttgca gattccgatt atctgggaat cggtactctg
     9241 gagtcattta aaaagatgcg taaatactat ggggaagatg cagtctgtcc tgtttatgtc
     9301 caggtagaag atggagaaag actgagtcgt gctcttaata gagagagaga acaggaaaat
     9361 ccaagatatg aggaaatgtg tagaagattt attgcagatc agtcggattt ttcagaggaa
     9421 aatattctga acgcagggat agagaaacgt tttcagaata taaatcttga tgactgtgtg
     9481 aaagaaatcg caaattatat aaagtctgtg caatagttct tcttttatgg tataatatct
     9541 tacaaaacgg actggtgagg tgattcttat gatgggattt aacaatatta tcggacacga
     9601 agagattatc ggacatttaa aaaatgcaat agaatcagga aaaatatcgc attcttatat
     9661 ttttaccggc gaaccaggtt ctggtaagaa gctcctcgca gggacttttg cgtcaacact
     9721 tcagtgtgag gccggaggta cagaaccctg ccagaaatgc gactcctgca aaaaggcgat
     9781 gggtaagaat catccggaca ttattatggt cagtcatgag aaacctggaa cgatcacaat
     9841 tgacgagatc agagatcagg tgatcaatga tatagatatc agaccatatt acagtcctta
     9901 caagatttat attattgcag atgcagatct tatgacacca caggcacaga atgctcttct
     9961 taaaacaatt gaagagccgc cggaatatgc agtgattctt ttgctgacta ataatatcgg
    10021 gggactgctg ccgactattc agtcaagatg tgttcgtctt gatctgaagg tagtggatga
    10081 cggtcttgta aagaaatatc tgatggagca tcttcatgtt ccggattacc aggcggagat
    10141 tgacgcatcc tttgcacagg gcagtattgg aagggcaaaa gaagcagcta cttctcagga
    10201 gtttgcagag atgactcaga atgcactgag gattcttaaa tatgcaaaca ccatggaagt
    10261 ttatgaactg tcagatgcga ttaagagtct ttctgaggac aaacagaata tcaacgatta
    10321 tctggatatt ttccagttct ggttcagaga tgtcctgatg tttaaggcaa cacaggagat
    10381 tgacaatctg gtatttaaac aggaaattaa ttttatcaga gaacaggcaa agcagcgttc
    10441 ctacgagaat ctggagaaca ttctggattc tattcagaag acaaaggtac gtctgaaagc
    10501 aaatgttaat tttgatctgg catttgaatt gctgttcctg acgatcaggg agaaatagaa
    10561 tgacaaaagt agtaggcgtg agatttcgta atgtaggcaa gatttattat ttcagtccta
    10621 aagattatga aattaaaaca ggcgatcacg tgatcgtgga gacagcccgg ggaattgaat
    10681 acgggaaagt cgtacttgca ccaagagaag tagatgagga agatgttgtt caccctctca
    10741 aagaagttct tcgtgtggca acaaaggagg acgacgagag agaggcacag aacagagtca
    10801 gagagagaga ggcatttaaa atctgtcaga agaagattcg tgaacatggt ctggaaatga
    10861 aactgataga tgcagagtat acatttgata ataataaagt gctgttttat ttcactgctg
    10921 acggcagaat tgatttccgc cagttggtaa aagatctggc agcaattttt aaaacgcgta
    10981 tcgaacttcg ccagattggt gttcgcgatg agacaaagat tctgggagga atcggtatct
    11041 gcggaagatg tctgtgctgc cacacatatc tgtctgagtt tgcacctgta tccataaaga
    11101 tggcaaaaga gcagaatctg tcccttaacc agacaaagat ttccggtgtg tgcggaagac
    11161 tgatgtgctg cctgaagaac gagcaggaaa catatgaaga gctgaataaa aagcttccgg
    11221 gactgggaga tacagttaca actccggatg ggcttaccgg aactgtacac agtgtaaacg
    11281 tgcttcgtca acgtgtaaag gtaattgtag agatcaatga cgagaaagaa attcaggaat
    11341 ttccggtaga tgacctgaaa ttcagaccga gaaagaagaa agtcaaagtc tctgaaaaag
    11401 aattaaaaga acttggaaat ctggaagata aaaaaggaga ttccaaatta aatgactgat
    11461 cagctggtaa aagataatga acgtatagat gatcttcaga acggatatta tgtgatacag
    11521 gatccggata aattctgttt tgggatggat gcagttcttc tttccggctt tgcaaaagta
    11581 aaaaagggtg agactgctct ggatctggga acaggaacgg gaattatccc gatcctgctt
    11641 aaaaccaaaa caaatgggaa acactttaca ggtcttgaga ttcagaaaga gtgtgcagat
    11701 atggccggac gcagtgtcag atataatcat ctggaggacg atgttgagat cgtacggggt
    11761 gatattaagg aggctgctga tatttttggg gcagcttctt ttgatgtagt gacaagtaat
    11821 ccgccttata tgatcggaca gcatggactt cgcaattcgg atatgccaaa agcaattgcc
    11881 agacatgaag tactctgtaa tctggaggat gtggtgagcc aggcatcaaa ggttctgaaa
    11941 gaacgtggaa gattttatat ggttcacaga ccgttcagac tggctgagat tatgaatttt
    12001 ctcacaaagt atcgtctgga accgaaaagg atgcagcttg tatatccata catagacaga
    12061 gaaccaaaca tggttctgat cgaggcatta aagggaggaa attccagagt tacagtggaa
    12121 cctcctctga tcgtatataa agagccggga gtctatacag aaaatatact gaagatttat
    12181 gatatgattt aaccgaattt tatttgcaaa gtaatatgag aggaaacaaa ttatgagtgg
    12241 taaattgtat ttatgtgcaa cacccatagg aaatctggag gatattacac tccgggtact
    12301 tcgaacattg aaagaagtgg atcttattgc agcagaggat accagaaaca gtattaaact
    12361 gttgaaccat tttgatataa aaacacctat gaccagttat catgagtaca acaaaattga
    12421 taaggcatat gttctgatca gtaaaatgca ggaagggcag aatattgcac tgatcacaga
    12481 tgcgggaact ccggggatct cagatccggg agaggagctt gcggcaatgt gttatgaagc
    12541 ggggattgaa gtaacttctc ttccgggacc tgctgcctgt atcactgccc tgactctgtc
    12601 cggacttcca accagaagat tcgcttttga agcatttctt ccggcagata aaaaagaaag
    12661 aaaactgatt ctggaagaac tgaagaatga aacgagaact atcattctct atgaggctcc
    12721 tcacagactg ataagaactc tggaggaatt gaaggaaact ctcggaaatc gcagaatgac
    12781 tctttgcagg gaactgacga aaagacatga gactgcattc catacgacta tcgaggagct
    12841 gatcctctat tatcagacag agaagccgct tggagagtgc gtgctggtga ttgaaggcag
    12901 aagcagacag gagatggaag aggaacagaa ggcatcctgg gaaaaaatta ccatagaaga
    12961 acacatggaa atctatgaaa atcagggaca ttcaagaaaa gaagctatga aaatggtagc
    13021 aaatgacaga ggaatgacga agagagatgt ctatcagtac ctcatcaata aaaccgaaga
    13081 ataagattaa catacagaag aattcccgtt ttcggggatt cttttttatt caggctgtgg
    13141 caaggaacgg gcttttgtgc tataataaat ttgtattatt atgtccagat accagaatat
    13201 atttgaaaac ctgtctataa ttttcacgca ggggttttca gatatattct ggtgataagt
    13261 atatataaca ggaggaaaga aatgggcgga gaaaatacag aatacggagc ggatcaaatc
    13321 cagattctgg aaggtttgga agccgtacga aagagaccag gtatgtatat cggaagtaca
    13381 tccggcagag gtcttcatca tctggtttat gagattgtag ataacgcggt tgatgaagca
    13441 ctggcaggat tttgtacaga gattcaggta gttattaatc cggacaactc tatcacagtt
    13501 gtagataacg gacgtggtat tccggtagga atcaatcata aagcagggat tcctgctgta
    13561 gaagtagtat ttaccattct tcatgccggc ggtaaatttg gaggcggagg gtacaaggta
    13621 tccggtggtc ttcacggagt aggtgcgtct gtagtaaatg cactttccac atggcttgaa
    13681 gtaaggatct atcatgaagg taagatttat cagcagagat atgaacgcgg taaaactatg
    13741 tatcctctga aggttgtggg agactgccaa ccggataaga ccggaacaac agttgtcttc
    13801 cttccggata aagaaatatt tgaggaaaca gtatatgatt atgacattct gaaacagcgt
    13861 ttgagagaga tggcattcct gacaaaggga ttgaagattt ctcttaaaga taccagagaa
    13921 ggaatggaaa gagagaaagt tttccattat gaaggtggta tccgtgaatt tgtaagctat
    13981 ctgaacagaa gcaaggaagc gctttacccg gaaatcattt actgcgaagg acagcagaac
    14041 ggtgtgtctg tagaggttgc catgnnnnnn nnnntgactc ttatacagag aactgctatg
    14101 gatttgtaaa taatattaat acacctgagg gtggtactca tattgtaggt ttcagaaatg
    14161 ccctgacaaa gacgttcaat gattatgcca gaaagaacaa gctgcttaaa gacagtgaac
    14221 ccaatttgag tggcgatgat atccgcgaag gtctgactgc aatcgtcagt gttaagattg
    14281 agaatcctca gtttgaaggt cagacaaaac agaagcttgg aaacagtgaa gcaagagggg
    14341 ctgttgacag cgtggtgagc aagcagcttg agattttcct tgagcagaat ccaacagtag
    14401 cgaaaactac agtagagaaa tcagttcttg cacagagagc acgtgaggct gcacgtaagg
    14461 ctcgtgatct gaccagaaga aaatcagcac ttgataatat gtcacttcct ggaaagcttg
    14521 cagactgttc agataagaat cctgagaact gtgaaatcta cattgtagag ggagattccg
    14581 ccggtggctc tgcaaaaaca gccagaaatc gtgtaacaca ggctatcctt ccactgagag
    14641 gtaagatcct gaatgtagag aaagcacgtc tggataagat ttatgcaaat gcagagatca
    14701 aagctatgat cacagctttc ggaacaggaa ttcatgaaga ttttgatatc agtaaacttc
    14761 gttatcataa gattattatt atgactgatg ccgatgtcga tggtgcgcac atcgcaaccc
    14821 ttctgctgac tttcctgtat cgttttatgc ctgagcttat caaacaggga tatgtgtatc
    14881 ttgcacagcc gccattatac aaggtggaga agaacaagaa agtatggtat gcatacgacg
    14941 atgatgaact gaatcagatt ctgacagaaa tcggacgtga cggaaataac aagatccagc
    15001 gttataaagg tcttggagag atggatgcag accagctttg ggagacgacg atggatcctg
    15061 agaaacgaat cctgcttaag gttaccatgg atgaggctgc gacttctgag attgacctta
    15121 catttactac actgatgggt gacaaggtag agccgagacg tgaatttatt gaagagaatg
    15181 caagatttgt aaagaatctg gatatctgat aatagggaga atagataaat ggaagataat
    15241 atttttgaca aagtccacga ggtggatctg gaaaagacca tgaaggactc ttatattgac
    15301 tatgccatga gcgttatcgc ttccagagca ctgcctgatg taagagatgg tctgaaaccg
    15361 gttcagcgac gtgtattgta ttccatgata gaactgaata atggtcctga caaaccgcat
    15421 cgtaaatgtg cgcgtattgt cggtgataca atgggtaaat atcatccaca tggtgacagt
    15481 tctatttatg gagcacttgt aaatatggca caggaatggt ctaccagata tccgctggta
    15541 gacggacatg gaaactttgg ttccgtagat ggtgacgggg cagcggccat gcgatacact
    15601 gaggcacgtc taagtaagat ttccatggaa atgctggcag atattaacaa agatactgtt
    15661 gacttccagc caaactttga tgaaacagag agagagccgg ttgtactgcc gtcccgttat
    15721 ccgaatcttc tggtgaacgg aacttcaggt attgcagtag gtatggctac caatatccct
    15781 cctcataatc tgagagaggt tgtggatgct gttgtcaaga ttatagacaa tatcattgaa
    15841 gaacaacgcg aaacgactat ggaagagatt ctggatattg taaaaggtcc tgattttcct
    15901 acaggtgcgt caggtaactg
//
LOCUS       DBMDXG010000008        13517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1327_length_13517_cov_2.224061, whole genome shotgun sequence.
ACCESSION   DBMDXG010000008 DBMDXG010000000
VERSION     DBMDXG010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 13517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13517
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1327_length_13517_cov_2.224061"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            17..463
                     /locus_tag="ACZPD3_00675"
     CDS             17..463
                     /locus_tag="ACZPD3_00675"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I 24 kDa subunit family protein"
                     /protein_id="HIR1414568.1"
                     /translation="MIAFYGKKPASLIPIMQDIQGVYRYLPEELLTYVAEQIGVTEAK
                     AFSVATFYENFSFDAKGKYVIKVCDGTACHVRKSIPVLEELYKKLGLSKTKKTTDDMM
                     FTVETVSCLGACGLAPTMMVNEEVYPRMTPEKADELIDKLRGGEQG"
     gene            460..2337
                     /locus_tag="ACZPD3_00680"
     CDS             460..2337
                     /locus_tag="ACZPD3_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR1414569.1"
                     /translation="MIQNKEVLEQIREEAVKELNSYDCRILVCSGTGCVATGSQKIYE
                     KFMEIAKDAPGVTIEFGPHDKDAHVGIKKTGCQGVCELGPLVRIQKGDDVIQYTKVQI
                     EDCQEIFEKSVQGNETIERLLYQKGGKVSRGPEDIPFIAKQTRIVLKNCGKFDAESLN
                     EYIASGGFQALEKAMFEMDPDLVIDQVDKSGIRGRGGGGFPAGKKWIQVARQADPVHY
                     VVCNGDEGDPGAFMDGSVMEGDPYRLIEGMMLAAYAVRAQHGYIYVRAEYPQSVARLK
                     HAIAVLEEVGLLGDDILGTGFSFHMHINRGAGAFVCGEGSALTASIEGSRGMPRVKPP
                     RTVEQGLWAKPTVLNNVETYANIPEIILKGADWYRSIGTEGSPGTKTFSLTGSIENTG
                     LIEVPMGTTLREIIFDIGGGLKSGAAFKAVQIGGPSGGCLTEEHLDVPLDFDSVKKYG
                     AIVGSGGLVVMDEHTCMVEVARFFMGFTQRESCGKCGPCRIGTKRMLEILERIVDGKG
                     EVEDLDKLEHLGNFIKDRSLCGLGKSAPLPVLSTLRNFRDEYVEHIVDKKCAAHVCKA
                     MRSYVIDPEKCKGCTKCARNCPVGAITGNKKEPHSIDTSKCIKCGTCLENCVFGAISV
                     N"
     gene            2351..5059
                     /gene="fdhF"
                     /locus_tag="ACZPD3_00685"
     CDS             2351..5059
                     /gene="fdhF"
                     /locus_tag="ACZPD3_00685"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015761027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR1414570.1"
                     /translation="MIHLTIDGIPVEVEKGTTILQAARQIGVEIPTLCYLEDVLPDGS
                     CRLCVVEVTNNGRTKFDTACTLRCSEGDEVQTMSEKVVAYRKDTLDLLLSDHRVHCFS
                     CEANGDCKLQDYCFEYGVTETSYPGEMKDMPIDDTNKFFTYDPSLCILCHRCVNTCKE
                     IVGRGAIDTMDRGFQSVIGAHYKHKWNEGICESCGNCVQACPTGALTMKRRKKYRPYQ
                     IDKKVLTTCPHCATGCQYYLLVKDGKIVDTEAVNGPSNKGLLCVKGRSGCFDFVQSPE
                     RIKYPLIKNKETGEFERATWDEALDLVASKFMEIRKQYGSDSLAGFACSRSPNEDIYM
                     VQKMVRCCFGTNNTDNCARVCHSASVSGLAMTLGSGAMTNPIEDITKNADLIMLVGSN
                     PEEAHPVVGMQIRQAIKRGCKLIVVDPRDIGLAKKADIHLKLKPGTNVAFANGIMNVI
                     LSEGLQDDKFIAERTEGFEELKEIVKDYTPEKVAEICHIDADDLRKAAIMYAKADRAP
                     IIYCLGVTEHSTGTEGVMSMSNMAMMVGKLGREGCGVNPLRGQNNVQGACDMGAQPNV
                     YPGYQKVTDPAVREKFEKAWGVKLDPNIGTHATDVFPKAITGEIKGLYIYGEDPVVTD
                     PDTTHIIKALKSLDFFVLQELFMTETAQYADVILPGVSYAEKEGTFTNTERRVQRVRK
                     AVTVPGEMRLDTDIIIDLMNRMGYPQPHLTSAQIMDEIASLTPSFAGISHERLDSEEV
                     HGQGLQWPCTSKDHPGTPIMHVGKFSRGLGWFYPAKYVPSAELPDEEYPIILMTGRIL
                     YHYTTRAMTGKTPELMEIEGKSFIEMNIVDADKLGIKNGDKVRVSSRRGSIESTARVG
                     TKTSPGESWMPFHFPDGNANWLTNAALDKYARIPEYKVCAIKIEKA"
     gene            5270..6994
                     /locus_tag="ACZPD3_00690"
     CDS             5270..6994
                     /locus_tag="ACZPD3_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014904.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR1414571.1"
                     /translation="MQRFQSILQFKKVSCKNCYKCVRNCPVKAIRVHDHQARIIESQC
                     IYCEKCILVCPQDAKEEQNMIPAICNAMENKTQVIASLHPAYLARFGVTGINGIREAM
                     KKLGFADAADAAEGASLMISQYRALFAEQKEPGIMISSACPVIVQLVKKHYPHLLGNL
                     VQSASMMQFHASYLKKKYPKAEIVYVSPCISAMSELRDSGNEVDYVITLEELVQWMKK
                     EGISITEKEPEQIAYRSREIAIADGLTDLLGAVKGIRRLSVSGMEQCREVLEELHPED
                     FENCFLEMYACSGGCVAGPSFQMKKGHYLADVLAVKNAAFGKDFHREQGDYELPGFEL
                     RKNFGYCSAQEQEEVSEEEIRDALAEMGKFSPKDELNCGACGYNTCREKAIAIIQNKA
                     EVAMCIPYMRAKQESYSNKVFNAMPGLLVTVDYNLKIIQMNEAASKLFNMPKKRRLIG
                     KPVSEIMDDYSLASILAFERNLMQDEIYLEDQKCYLDRVMTNDKENKMILCIMKDITK
                     ERKHKDQIHNAQIEAARMADKLVEEQLKIVQQIAGLLGETAADTKVAVEKLKNTILLE
                     SEEENEKK"
     gene            6981..8183
                     /locus_tag="ACZPD3_00695"
     CDS             6981..8183
                     /locus_tag="ACZPD3_00695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR1414572.1"
                     /translation="MKRNKHHGLNVEFDGLCMDFGHVSLNKKKEFLCGDCYKIEENDK
                     DHVLVLSDGLGSGVKANILSTLTATMLSTMIINQVELDEAVRAVAKTLPVCSVRNLAY
                     ATFTVLNFQGKQVSLYQFDNPDAILIRDGKLFDYPVETSMIEEKEIHKSCFELKDEDM
                     LIVMSDGVTNAGMGKTTNGGWGRDDVMAFCRAKYHKGMSAQEMAGYLAEASLDLNLNE
                     TDDDITAIVLRMRHKQVVNLMIGPPSKEEHDERYLKSFFDSEGYHVICGGTTAQCAAR
                     YLDKELISLSETACGDVPAYYKLEGTELVTEGFLTIEHLLEYCEEWMEDRLSFNRLKR
                     KKDAAALLGVMLFEQATDINFYFGGAINKSNVELGITEKRKEQAAEELVEHLQNAGKN
                     VNIAFWAI"
     gene            8247..8681
                     /locus_tag="ACZPD3_00700"
     CDS             8247..8681
                     /locus_tag="ACZPD3_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015438.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR1414573.1"
                     /translation="MAKILCVCISKERKTCSQNIHECEADLQGLVGDVHYGMGGRKQV
                     SLLPYEKVKEYFEQSEEEFRYGRFGENLLVEGIDWNSIAEGNRFRCGDVLLEAVRIGA
                     GGPASDAYKGEKVCTPMEPWFVFCQILEAGRLREGEIILQQR"
     gene            8921..10183
                     /locus_tag="ACZPD3_00705"
     CDS             8921..10183
                     /locus_tag="ACZPD3_00705"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR1414574.1"
                     /translation="MEGISVEQAVEQILQHTPVINETEETELNKAGGRILAQDMVAGF
                     DNPPFDRSPVDGYACKAEDLAGAAKEHPVRLKVMEEIDAGQYSEREIQQGQAVRIMTG
                     AAIPNGCNCCIYQEDTDYGEDTVEVYSEQKRWSNYCFAGEDFKKGTTLLKKGTHIGYV
                     EAAILAGMGVAKVPVYRQPRIVLLTTGDEVVELGIPLPEGKIYNSNMTMLSARLRELG
                     IESFHMEAVKDDPTVMSEKLKEAAKAADMIITTGGVSVGKKDIMHESLRLMGAERIFW
                     RVKMKPGMPTLFSAYKKTSDITATDHCASEREIPIISLSGNPFGVAVSIELLIRPALE
                     KMMQDPSIGLKEVNGIMADNFEKGIKGRRFIRAYLENGKFHLPNGLHSNGVLSSMAGC
                     NCLIDTKTMENQESRSLNAGDKVGAILL"
     gene            10200..10712
                     /gene="mobB"
                     /locus_tag="ACZPD3_00710"
     CDS             10200..10712
                     /gene="mobB"
                     /locus_tag="ACZPD3_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR1414575.1"
                     /translation="MERKRIFAISGVKNSGKTTLICRLLEIFKDKGLKVAVLKHDGHD
                     FVPDVPGTDTYCQLQSGAYGTAVFSAGKYMLVKQQPQISEKELAEFFPEADLILLEGF
                     KYSTYPKIEIIRKGNSAESVCDPEKLMAIATNLDAEERDALSVLENVPFFELDNAECI
                     AEFILSDYFR"
     gene            complement(10687..11505)
                     /gene="fdhD"
                     /locus_tag="ACZPD3_00715"
     CDS             complement(10687..11505)
                     /gene="fdhD"
                     /locus_tag="ACZPD3_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR1414576.1"
                     /translation="MKINEQYHNLNLLENVTYEFLGRDGETHEETEPILVEHMMDVYV
                     NERLTMKLVCIPQHLTELVLGRLFTEGIISSAEDVEQIYICEFGKRARVILSKNKSGQ
                     SHEENEDYVALTPTCCTGNRVLNDYFITDQPMTPVTPVPWKKQWIFDLADCFANGTPL
                     HSQTWATHSCFLACDGELLFQCEDIGRHNALDKAIGYALRHNIDLKKCVVYSSGRIPT
                     DMAIKAIRAGIPVLASKASPSAEAVAMAKEYHLTLICAARRDRMKLFTGNNPTE"
     gene            11716..12690
                     /gene="moaA"
                     /locus_tag="ACZPD3_00720"
     CDS             11716..12690
                     /gene="moaA"
                     /locus_tag="ACZPD3_00720"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR1414577.1"
                     /translation="MIDKYGREIDYLRISLTDRCNLRCIYCMPEEGVKSLSHAEILTY
                     DEILRICRCAADLGIRKIKLTGGEPLVRKGCASLAKQIKAIPGIDKVTLTTNGILLAE
                     QLDALLDAGIDAINISLDTLDPELFKKVARRDGLEKVFEGIKAALAHPGLSLKINSVP
                     VIKEKENFIGIAGLAQKYPIHVRFIEMMPIGFGKQFPFQDEESIKAVLEEAYGPMHAV
                     NERYGNGPCHYYEIAGFKGKIGFISAMTHKFCSQCNRVRLTSEGFMKGCLQYQKGTDL
                     RGLMRGGCTDSELKKAIYQVIWNKPVSHNFYEAKTEQDEIRGMSQIGG"
     gene            12805..13398
                     /gene="mobA"
                     /locus_tag="ACZPD3_00725"
     CDS             12805..13398
                     /gene="mobA"
                     /locus_tag="ACZPD3_00725"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR1414578.1"
                     /translation="MRKKEISMIILAGGASSRMGRDKSDLTIDGKTFLEMQIEKGEKL
                     GISDILLSGYHGENKYKYPIIPDRFPGKGPLGGLEACFRKAKNPYCLVLGVDVPLVPA
                     EELTALIRQSLHSDAKAVILSHSGHEEPLMGVYCTDLADAMLEEITERKGAVFAFLRK
                     NGYECYESQVADWYFSNINDPETYKEIPGNHFIEKYV"
BASE COUNT         4072 a         2528 c         3553 g         3364 t
ORIGIN      
        1 tcagtaagcg gatgagatca ttgctttcta tggtaaaaag ccggcatcac tgattccgat
       61 catgcaggat attcaggggg tgtacagata tctcccggaa gaattgctta catatgttgc
      121 ggagcagatc ggtgtgacag aagcgaaagc gttcagtgtt gccacattct atgagaattt
      181 ctcatttgat gcgaaaggta aatatgtgat caaggtctgt gacggaacag cctgtcatgt
      241 aagaaaatca ataccggttc tggaggaact ttataagaaa ctggggctgt caaagacaaa
      301 gaagacgaca gacgatatga tgtttactgt ggaaacagtt tcctgtcttg gtgcctgcgg
      361 acttgcaccg actatgatgg tcaatgaaga ggtatatccg agaatgactc cggagaaggc
      421 agatgagctg attgataaac tgagaggggg cgaacaggga tgatccagaa taaggaagta
      481 ttggagcaga tcagagaaga agctgtgaaa gaacttaatt cttatgactg tcgtattctt
      541 gtctgttcag gtaccggatg tgtggcaacc ggttctcaga aaatctatga gaaattcatg
      601 gagattgcca aagatgctcc gggagttacc attgagtttg gacctcatga caaagatgca
      661 catgtaggta taaagaaaac aggatgtcag ggtgtctgtg aacttggacc acttgttcgt
      721 atccagaaag gcgacgatgt gatccagtat acgaaggttc agatcgaaga ctgccaggag
      781 atttttgaga agagtgttca gggtaatgag actatcgaga gactgcttta tcagaaaggc
      841 ggtaaagtaa gccgcggacc ggaagatatt ccgtttatcg cgaaacagac ccgtattgtt
      901 ttgaagaact gtggtaagtt tgatgcagaa tctctgaatg agtacatagc aagcggcggt
      961 ttccaggctc tggagaaagc aatgtttgag atggatccgg atctggttat tgatcaggtt
     1021 gataaatccg gtatccgcgg acgtggtggc ggcggcttcc cggcaggtaa gaaatggatt
     1081 caggtagcac gccaggctga tcctgttcat tatgtggttt gtaacggtga tgagggtgac
     1141 ccgggagcat ttatggatgg ttctgtaatg gaaggtgatc cttaccgtct gatcgagggt
     1201 atgatgcttg cagcttacgc agtaagagca cagcatggtt atatttatgt acgtgcggag
     1261 tatcctcagt ctgtagcacg actgaaacac gctatcgcag ttctggaaga agtgggactg
     1321 ttaggtgatg acatccttgg aacaggattc tccttccata tgcatatcaa ccgtggtgca
     1381 ggtgctttcg tatgtggtga aggttctgcc ctgactgcat ctatcgaagg aagcagaggt
     1441 atgccccgtg taaaaccgcc ccgtacagtt gaacagggat tatgggcgaa acctactgtt
     1501 ctgaataacg tagaaactta tgcaaatatt ccggagatca ttctcaaagg agctgactgg
     1561 tatagaagta tcggtacaga gggatcacct ggaacaaaga ccttctcact gacaggatcc
     1621 attgagaata caggtctgat cgaggttccg atgggaacaa ctttaaggga aatcattttt
     1681 gatatcggcg gaggactgaa atccggtgct gcgtttaagg ctgttcagat cggtggacca
     1741 tccggtggat gtctgacaga agaacatttg gatgtacctc ttgatttcga ctctgttaag
     1801 aaatatggtg cgatcgttgg ttccggtggt ctggtagtta tggatgaaca tacctgtatg
     1861 gtagaggttg cacgtttctt tatgggcttc acccagagag aatcctgcgg taaatgtggt
     1921 ccctgccgta ttggcaccaa gcgtatgctt gaaatccttg agaggatcgt agacggaaag
     1981 ggtgaagtag aggatctgga taaactggag catcttggaa actttatcaa ggaccgttca
     2041 ctctgcggac ttggaaagag tgcaccgctt cctgtacttt ccacattaag aaacttcaga
     2101 gatgaatatg tagagcatat tgttgataaa aagtgcgcgg ctcatgtatg taaggcaatg
     2161 cgttcttatg tgatcgatcc tgagaaatgt aaaggatgta ccaagtgtgc gagaaactgt
     2221 ccggttggtg cgatcacagg caataagaaa gagcctcaca gcattgatac ctccaagtgt
     2281 attaagtgcg gaacatgtct ggaaaattgt gtattcggcg caatttctgt gaattaagga
     2341 gggtgaaaag atgattcatt tgacaataga tggaattcct gtagaagtgg aaaaaggtac
     2401 gaccattctg caggctgcca gacagatcgg agtggagatt cctacactct gctatctgga
     2461 agatgtattg ccggatggtt cctgtcgttt gtgcgtggta gaagtaacaa acaatggaag
     2521 aaccaaattt gatacggcat gtacacttcg ctgctcagag ggtgatgaag tacagacaat
     2581 gtcagagaaa gtagttgcat atcgtaaaga tacccttgac ctccttcttt ctgaccatcg
     2641 tgtacactgc ttctcctgtg aggcaaacgg tgactgtaaa ttacaggact actgttttga
     2701 atatggtgtg actgagacaa gctatcccgg tgaaatgaag gatatgccga tagatgatac
     2761 caataaattc tttacctatg atccaagcct gtgtattctc tgccatcgct gcgtgaatac
     2821 atgtaaggag attgtgggaa gaggtgccat cgacactatg gatcgtggtt tccagtcagt
     2881 gatcggtgca cattacaaac ataaatggaa cgaaggtatc tgtgaatcct gcggtaactg
     2941 tgtacaggcc tgtccgacag gtgcactgac catgaagcgc agaaagaaat accggccgta
     3001 ccagattgac aagaaggttc tgactacctg tcctcactgt gcaacaggct gccagtatta
     3061 tctccttgta aaagacggaa agatcgtaga tacagaagct gtaaacggac catccaacaa
     3121 aggattgctc tgtgttaagg gccgcagcgg atgcttcgat ttcgtacagt caccggagcg
     3181 tattaaatat ccgctgatca agaataaaga aacaggtgaa ttcgaacgtg caacatggga
     3241 tgaggcgctg gatcttgttg cttccaaatt tatggagatc aggaaacagt acggctctga
     3301 ttctcttgca ggttttgcat gctccagatc accgaatgaa gatatttaca tggtacagaa
     3361 gatggttcgc tgctgcttcg gcaccaacaa tacagacaac tgtgcacgtg tatgtcattc
     3421 tgcatctgta tcaggtcttg cgatgactct tggctcaggt gccatgacaa acccaattga
     3481 ggatatcaca aagaatgcag atctgatcat gctggttggt tccaaccctg aggaagcaca
     3541 tcctgtagtc ggcatgcaga tccgtcaggc tatcaagaga ggctgcaagc tgatcgtagt
     3601 tgatcctcgt gatattggtc tggctaagaa agcagatatc catctgaaac tgaaacctgg
     3661 tacaaatgtt gcttttgcaa acggcatcat gaatgtgatc ctttctgaag gtcttcagga
     3721 tgacaagttc attgcagagc gtaccgaagg ttttgaagaa ttaaaggaaa tcgtaaaaga
     3781 ttacacacct gagaaggtag ctgaaatctg ccacattgac gcagacgatc tgagaaaagc
     3841 agcgatcatg tatgcaaaag cagaccgcgc tccaatcatc tactgccttg gtgtaaccga
     3901 acattccaca ggtacagagg gcgttatgtc catgtccaat atggcaatga tggttggcaa
     3961 gcttggacgt gaaggctgtg gtgttaaccc gcttcgtgga cagaataacg tacagggtgc
     4021 ctgcgatatg ggcgctcagc cgaatgtata tcccggatat cagaaggtta ctgatcctgc
     4081 agttcgtgag aaattcgaga aagcatgggg tgtaaaactt gatccgaata tcggaactca
     4141 tgcaactgat gtattcccga aagcaatcac aggcgagatc aaaggtctgt atatctatgg
     4201 tgaggacccg gtagttactg atccggatac cacacacatt atcaaagcat taaagagtct
     4261 tgatttcttt gtacttcagg aacttttcat gacagagaca gctcagtatg cagatgttat
     4321 ccttcctggt gtaagctatg cagagaaaga gggtacattt accaatacag agcgccgcgt
     4381 acagagagtc cgcaaggctg tcacagtacc tggagagatg agactggata cagatattat
     4441 tattgatctg atgaaccgca tgggatatcc gcagccacat ctgacttctg ctcagatcat
     4501 ggatgagatc gcatccctga caccgagctt cgcaggtatc agccatgaac gtcttgacag
     4561 tgaagaagtt catggacagg gacttcagtg gccatgtacc tccaaggatc atccgggaac
     4621 accgatcatg catgttggta aattctcccg cggtcttggc tggttctacc ctgcaaaata
     4681 tgtgccgtca gcagagcttc cggatgagga atatccgatc atccttatga caggacgtat
     4741 tctgtatcat tacacaacaa gagccatgac aggaaagaca cctgagctta tggagatcga
     4801 aggtaagtca tttatcgaaa tgaacatcgt agatgccgat aagctgggta tcaaaaacgg
     4861 tgataaagtc agagtttctt ccagacgagg atccattgaa tctaccgctc gtgtaggtac
     4921 aaagacctca ccgggtgaga gctggatgcc attccacttc ccggatggaa atgcaaactg
     4981 gctgaccaac gccgcacttg acaaatacgc aagaattcct gaatataagg tatgcgcaat
     5041 taagattgag aaagcataaa acaatatcgg agacaaagtt ccggatctga tatcgtaaat
     5101 attatatggg gctgtcgcac atggatctgc tgtccttgcg gcagctccat attcctttat
     5161 ggaaaggcaa aaataatcat tcggcagtac atcattctgt gaaagtacgg aacggttaca
     5221 gataattttt tgtgaaaaat atagtgttac ataataagga gtacgaggca tgcagagatt
     5281 tcagtcaatc cttcagttta aaaaagtcag ttgtaaaaat tgttacaaat gtgtccggaa
     5341 ctgcccggtc aaggcgatac gtgttcatga tcatcaggcc aggatcatag aaagccagtg
     5401 tatttactgc gagaagtgta tccttgtctg cccgcaggat gccaaggaag agcagaatat
     5461 gattcctgca atctgcaatg caatggagaa taagacgcag gttatagcct ctcttcatcc
     5521 ggcatatctg gcacgttttg gggtaaccgg tatcaatgga ataagggaag cgatgaagaa
     5581 gctgggattt gcagatgcag cggatgcagc agaaggtgca agcctgatga tttcgcagta
     5641 cagagcactt tttgcggaac agaaagaacc gggaataatg atctcttctg cctgccctgt
     5701 aatcgtacag cttgtcaaga aacactatcc gcatcttctg ggaaatctgg tacagtctgc
     5761 ctctatgatg cagtttcatg ccagttatct gaaaaaaaaa tatccgaagg cagagattgt
     5821 ctatgtaagt ccctgtattt cagctatgtc agagttgaga gactcaggaa atgaagtgga
     5881 ttatgtgatc acattggaag agctggtgca atggatgaaa aaagaaggga tttccataac
     5941 tgagaaagaa cctgagcaga ttgcctaccg ttccagagaa atcgccattg cggatggttt
     6001 aacagacctg cttggggctg taaagggaat ccgcaggctt tctgtaagtg ggatggagca
     6061 gtgcagggag gttctggaag aacttcatcc ggaggatttt gaaaattgct ttcttgagat
     6121 gtatgcatgc agcggcggct gtgtggcagg tccttctttc cagatgaaga agggacatta
     6181 tctggcagat gttctggcag tgaagaatgc agcctttgga aaagattttc acagagagca
     6241 gggagactat gaacttccgg gatttgagct gagaaagaat ttcggttact gttctgcaca
     6301 ggaacaggaa gaggtctcag aggaagaaat cagggatgct ctggcggaaa tgggaaaatt
     6361 ttctccaaag gatgaattaa actgcggtgc ctgcgggtac aacacatgca gggaaaaggc
     6421 aattgccatt atccagaata aagctgaggt tgccatgtgc attccatata tgcgggcaaa
     6481 acaggaaagt tactccaata aagtctttaa tgccatgccc ggacttctgg taactgtgga
     6541 ttataatctg aagatcattc agatgaatga ggcggcgtcc aaattgttca atatgccgaa
     6601 aaagcggcgc ctgatcggga aaccggtatc ggagatcatg gatgattaca gtctggcgag
     6661 tatactggct tttgaacgga atctgatgca ggatgagatt tatctggagg atcagaaatg
     6721 ttatctggat cgcgtaatga cgaatgataa agaaaataaa atgattctct gcattatgaa
     6781 ggatattaca aaggagcgga agcataaaga tcagattcat aatgcccaga ttgaagctgc
     6841 gcgtatggca gataagctgg tagaagaaca gctgaagatc gtgcagcaga tcgccggact
     6901 tcttggagaa actgctgcag acaccaaagt ggcagtagaa aaactgaaaa ataccatact
     6961 tctggagagt gaagaagaga atgaaaagaa ataagcatca tggactgaat gtggaatttg
     7021 acggcttatg catggatttt ggtcatgtga gcctgaataa aaagaaagaa tttctctgtg
     7081 gggactgcta taagattgaa gaaaatgata aagaccatgt tctggtgctt tctgacggac
     7141 ttgggagcgg agtaaaggcg aatatccttt ccacgctgac tgcgaccatg ctcagtacca
     7201 tgatcattaa tcaggtggaa ctggatgagg cagtgcgtgc agtggcaaaa acgcttcctg
     7261 tgtgcagtgt gagaaatctg gcatatgcca cctttacagt gctgaatttt caggggaaac
     7321 aggtaagcct ttatcagttt gataatccgg atgcaattct gatccgggat ggcaagctct
     7381 ttgattatcc ggtggagaca agtatgatcg aagagaagga aatccataaa agctgttttg
     7441 aactgaaaga tgaggatatg ctgatcgtta tgtccgacgg tgtcaccaat gcaggaatgg
     7501 gaaaaactac caacggcggc tggggaagag atgatgtaat ggctttctgc agggcaaagt
     7561 accataaagg gatgagcgca caggagatgg caggctatct ggcagaggcc agcctggatc
     7621 tgaatctgaa tgaaacggat gatgatatta cagccattgt cctgcggatg cggcataaac
     7681 aggtggtaaa ccttatgatc ggcccgccaa gtaaggagga gcatgatgag cgatatctga
     7741 agtccttttt cgattcagag ggatatcatg taatctgcgg cggaactact gcacagtgtg
     7801 ctgccagata tctggataaa gagctgatct ctcttagtga aactgcctgc ggagacgttc
     7861 ctgcctatta taagctggaa ggtacagagc tggtgacaga gggatttctt acgattgagc
     7921 atttgcttga atattgtgaa gaatggatgg aggacaggct gtcctttaac aggctgaaac
     7981 gaaaaaaaga tgcggcagca ttgcttggtg tgatgctgtt tgagcaggca acggatatta
     8041 atttctactt cggtggtgct atcaataaga gtaacgtgga actgggaatc acagaaaaaa
     8101 ggaaagaaca ggcagcagaa gaacttgtgg agcatctgca gaatgcagga aagaatgtga
     8161 atattgcatt ctgggcgatc tgagaaagca ggaaaaacaa cagtacatgg ttttataaag
     8221 agacatcagg aaggatgaga cagaaaatgg cgaagatatt atgtgtatgc atcagtaagg
     8281 aacgcaagac ctgctcgcag aatattcatg aatgcgaagc tgatctgcag ggacttgtgg
     8341 gagatgtgca ttatggaatg ggcggaagaa agcaggtcag tcttcttcct tatgagaagg
     8401 taaaagaata tttcgaacag tccgaagaag aattcagata tggccggttt ggagaaaatc
     8461 tccttgtgga agggattgac tggaacagta ttgcggaggg gaacagattt cgttgtggag
     8521 atgtacttct ggaagcagtc aggatcggtg ctggcggtcc tgcatcagat gcttataagg
     8581 gagaaaaggt atgcacgccc atggaaccct ggtttgtatt ctgtcagatt ctggaggcag
     8641 ggaggctgag agaaggagaa atcattctcc aacagagatg aaaaatatgt aattcttcag
     8701 cagtgggctg aatattatga tgttatagca atttccgaaa tactgaagac aatctggttt
     8761 atgaaaaata aggctctaat ggagtatctt agtgatatta ttctctgaaa ttgctataga
     8821 gttcaaaagg taaaactaat atatccgtca attgacccgg tggttataaa ggcattgaaa
     8881 agactataaa aatgtaatat acaagaggag gaattagtgc gtggagggaa tcagtgtaga
     8941 acaggcagtg gaacagattc tgcagcatac gcctgtaata aacgagacag aagaaacaga
     9001 actgaataaa gcagggggaa gaatcctggc gcaggatatg gtggcagggt ttgacaatcc
     9061 tccttttgac cgctctccgg tagatggcta tgcgtgtaag gcagaggacc ttgcaggtgc
     9121 cgctaaagag catccggtga ggctgaaagt catggaagag attgatgcag gacagtattc
     9181 ggaaagagaa atccagcagg gacaggcagt gcgcattatg acaggtgctg caatacccaa
     9241 tggctgcaat tgctgtattt atcaggagga taccgattat ggcgaagata cggtagaagt
     9301 ttacagcgaa cagaagcgat ggagtaatta ttgcttcgca ggagaggatt ttaaaaaggg
     9361 aactacactt ctgaaaaaag gaacacatat cggatatgtg gaggcagcaa ttctggccgg
     9421 aatgggagtg gcgaaagtgc cggtttaccg tcagccgaga atcgtgcttc tgactacagg
     9481 agatgaggtg gtagaactgg gaattccatt gccggaagga aaaatttaca acagcaatat
     9541 gacaatgctg tctgccagac tgcgtgaact gggaatcgaa tctttccata tggaagcagt
     9601 caaagatgat ccgacggtta tgtctgagaa attaaaagaa gcagcaaaag cggcagatat
     9661 gatcatcact acgggaggtg tatctgtagg caagaaggac attatgcatg agtccctgag
     9721 actgatgggg gcagagcgga ttttctggag ggtaaagatg aaaccaggaa tgcctacact
     9781 gttttctgca tataaaaaaa cttccgatat aactgcaaca gatcattgcg cttcagaaag
     9841 agagattccg atcatcagtc tctccggaaa tccttttggt gttgcagtca gcatagaact
     9901 tttgattcgt cctgcattgg aaaaaatgat gcaggatcca tcaatcggtc tgaaagaagt
     9961 aaatggcatt atggctgata actttgaaaa aggaatcaag ggccgccgct ttatccgggc
    10021 atatctggaa aatggaaaat ttcatcttcc caatggtctt cactctaacg gagtcctcag
    10081 ttccatggcc ggctgcaatt gcctgattga tacaaaaacc atggaaaatc aggaaagcag
    10141 aagcctgaac gcaggggata aagtgggcgc gattttgctt tgagaggagc agcaaacagg
    10201 tggaaagaaa aagaatattt gcaatcagcg gtgtaaagaa ttcagggaaa acaactctga
    10261 tctgcaggct tttggagatt tttaaagaca agggccttaa agtggcggtg ctgaagcatg
    10321 acggacatga tttcgtgccg gacgtaccgg gaacggatac atactgtcag cttcagtccg
    10381 gagcctacgg cacagcagtt ttttcagcag gcaaatatat gctggtaaaa cagcagccgc
    10441 agatttccga aaaagagctg gcagaatttt ttccggaggc agatctgatc ttactggaag
    10501 gatttaaata cagtacttat ccgaaaattg aaattatccg taagggaaat tcggcagaaa
    10561 gtgtctgcga tccggaaaaa ctgatggcga tcgccacaaa cctggatgca gaggaaagag
    10621 atgcactgtc tgtactggaa aatgttccat tctttgaact ggacaatgca gagtgtattg
    10681 cagaatttat tctgtcggat tatttccggt aaagagtttc atacggtcac gtcgcgcggc
    10741 acagataagg gtaaggtggt attctttggc catggcaact gcctcggcgg aaggggatgc
    10801 cttgcttgcc agaaccggta ttccggcgcg gattgccttg atcgccatat ctgtagggat
    10861 acgtccactg gaataaacca cgcatttctt caggtcaata ttatggcgca gggcataacc
    10921 gatggcctta tccagagcat tatgacgtcc gatatcttcg cactgaaaga gcagttctcc
    10981 gtcacaggca agaaaacagc tgtgagttgc ccaggtctgg ctgtgaagcg gggtaccatt
    11041 ggcgaaacag tctgcaagat caaagatcca ctgttttttc catgggacag gtgtaactgg
    11101 cgtcattggc tgatctgtga taaaataatc attaagaaca cgatttccgg tgcagcaggt
    11161 cggagtaaga gcaacataat cttcattttc ctcatgagac tgcccggatt tatttttact
    11221 caggatcacg cgggcacgtt tgccgaattc acagatgtag atctgctcca catcctcggc
    11281 agatgagatg ataccttccg taaagaggcg gccgagaacc agttctgtca gatgttgtgg
    11341 gatacagacc agtttcatgg tcaggcgttc atttacatat acatccatca tgtgttccac
    11401 aagaatgggt tccgtttctt catgagtttc cccgtcacga cccagaaact cataggtaac
    11461 attctcgagc agattcaggt tgtggtattg ttcgttgatt ttcataggac agttcctttc
    11521 gtccggtaag gattttaata atcttgatac attaagtata gactgcggag tatggagtgt
    11581 caaacgatta tgagagaaaa tattgcctgt gattttgacg gcgaataaca gaagaaaagg
    11641 ttacaaaggt tgcggttcac ccgtatccgg taacagaaaa atcaattatt agaaagtaaa
    11701 aggcaggtag agccgatgat agacaaatat ggaagagaaa tagattattt aaggatttcc
    11761 ctgacagaca gatgtaatct gagatgcatt tactgtatgc cggaagaagg cgtaaaatcc
    11821 ctttctcatg cagaaatcct cacatacgat gagattctcc gcatctgcag gtgcgcagca
    11881 gatctgggaa tccgtaagat taaactgaca ggcggtgagc ctcttgttcg taagggctgt
    11941 gcaagtcttg caaaacagat caaggcgatc ccgggaattg ataaagtgac attaacaaca
    12001 aatggaatcc tgctcgccga gcagctggat gcattactgg atgcaggaat tgacgccatt
    12061 aatatcagtc tggatacttt ggatccggaa ctgttcaaaa aagtagcgag acgtgacgga
    12121 ctggaaaagg tatttgaggg aatcaaagca gcacttgcac atccgggact ttccctgaag
    12181 atcaacagcg ttccggtgat caaggaaaaa gaaaacttca tcgggattgc aggactggcg
    12241 cagaaatatc caatccatgt gcgttttatc gagatgatgc caatcggatt cggaaaacag
    12301 ttcccattcc aggatgaaga atctatcaaa gcagtactgg aagaagccta cggtccgatg
    12361 catgcagtca atgaacgcta cggaaacggc ccatgccatt actatgaaat cgcaggattt
    12421 aagggaaaaa tcggatttat cagtgccatg actcacaaat tctgcagtca gtgcaaccga
    12481 gtacgtctta catccgaagg attcatgaaa ggatgcctcc agtatcagaa aggtacagac
    12541 ctgcgggggc ttatgcgcgg cggctgcaca gattcggaac tgaaaaaagc aatttatcag
    12601 gtgatctgga ataaaccggt gagccataat ttttatgaag cgaagacaga gcaggatgaa
    12661 atcagaggaa tgtcacagat tggcgggtga gatatgtaag acaccctaac cgttatgcag
    12721 acatttctgt ctgtagtttt taaatatgaa gaaaaaatca atgcttttat tgatgattta
    12781 tataaacaaa aaggaaaact gataatgcga aagaaagaaa tttctatgat tattcttgct
    12841 ggcggtgcca gcagccgcat gggacgggac aaatcagatc tgacgataga tgggaagaca
    12901 tttcttgaga tgcagataga aaagggggaa aagcttggaa tcagtgatat ccttctgtct
    12961 ggctatcatg gtgaaaataa atataaatat cccattatac cagaccgttt cccgggaaaa
    13021 ggtccgttgg gagggctgga agcctgtttt cgtaaagcaa agaatccata ttgcctggta
    13081 cttggtgtag atgttcctct ggttccggca gaggaactga ctgcactgat tcgccaatct
    13141 ctgcattcag atgcaaaagc agtgatactg agccacagcg ggcatgagga acctcttatg
    13201 ggagtatact gtacagacct ggcagatgcc atgcttgaag aaatcacaga gcggaaaggc
    13261 gcagtctttg cttttctgcg gaaaaatgga tacgaatgct acgaaagcca ggtcgcggac
    13321 tggtattttt ccaatattaa tgatccggaa acatataaag aaataccggg aaatcatttc
    13381 atagagaaat atgtctgaat atgagattgg caggaggttt gtcgatattt tcaaaaatta
    13441 gtagaaaata ttatattaag aagcgaagga accttgcagg tatgcaggtt ccttcgtctt
    13501 ttaaccgaat caagtaa
//
LOCUS       DBMDXG010000009        13105 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1379_length_13105_cov_1.918102, whole genome shotgun sequence.
ACCESSION   DBMDXG010000009 DBMDXG010000000
VERSION     DBMDXG010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 13105)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13105)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13105
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1379_length_13105_cov_1.918102"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..206)
                     /locus_tag="ACZPD3_00730"
                     /pseudo
     CDS             complement(1..206)
                     /locus_tag="ACZPD3_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595901.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
     gene            complement(338..1255)
                     /locus_tag="ACZPD3_00735"
     CDS             complement(338..1255)
                     /locus_tag="ACZPD3_00735"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1414579.1"
                     /translation="MQKTALVIMAAGIGSRFGKGIKQLAPVGPCGEIIMDYSIHDALE
                     AGFNKVVFIIRKDLEEEFRRVIGERIEKITEVEYVFQELDDLPEGFTKPADRTKPWGT
                     GQAVLAAKKVLDEPFIVINADDYYGKEAYVKVHDYLVQEQPKDGKLHICMAGFRLGNT
                     LSDNGSVTRGICHIKNGQLTGVTETHDIFKTATGAESRNADGSVQELDVKDLVSMNMW
                     GLTPDFMEILEKGFSEFLSGLAPEDTKKEYLLPELVDHLIKNESAEVDVLETKDTWFG
                     VTYQEDKETVMRAFKNLTEAGIYPQGLYQ"
     gene            complement(1336..2412)
                     /gene="prfA"
                     /locus_tag="ACZPD3_00740"
     CDS             complement(1336..2412)
                     /gene="prfA"
                     /locus_tag="ACZPD3_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR1414580.1"
                     /translation="MFDKLDDLLIRFEEVLNELGEPGVTDNQEHFQKLMKEQSDLQPI
                     VDAYREYKKNKETIQDSLSMLEEEKDEDMREMLKEELSEAKKNVEELEHELKILLLPK
                     DPNDNKNVIVEIRAGAGGDEAALFAAEIYRMYVKYAESRRWKTEMMSLNENGIGGFKE
                     VTFMITGAGAYSRLKYESGVHRVQRVPETESGGRIHTSTCTVAIMPEAEEIDFHLDMN
                     DCKFDVFRASGNGGQCVNTTDSAVRLTHIPTGIVISCQDEKSQLKNKDKALKVLRSRL
                     YEMELAKQHDAEAEARRSQIGTGDRSEKIRTYNFPQGRVTDHRIKLTLHRLENVLNGD
                     LDEIIDSLIAADQAAKLSNLQDAE"
     gene            complement(2405..3370)
                     /gene="prmC"
                     /locus_tag="ACZPD3_00745"
     CDS             complement(2405..3370)
                     /gene="prmC"
                     /locus_tag="ACZPD3_00745"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR1414581.1"
                     /translation="MNGETLTGLLKKGQMILAQAGIKEAGLDAWLLLEYVTGKSRAYY
                     FAYGEESVTESVAERYLELISRRAGHIPLQHLTHQAFFMGHEFYVDKNVLVPRQDTET
                     LVESALECMKTVKNPYILDMCTGSGCILISILKERADAHGTGVDLSDEALKVAVRNAR
                     TLEVAEHAEFVQSNLFSEMQNIVYGTEYMKRTAVKDTVKMTECENSNRNYSRAYDMII
                     SNPPYIPTAEIEDLMDEVKLHDPRMALDGMEDGLYFYRAITKQAQDHLVPGGWLLYEI
                     GCSQGEDVAALLRKYKFEDIEIRQDLAGLDRVVLGRKKLQEDKYV"
     gene            complement(3367..4335)
                     /locus_tag="ACZPD3_00750"
     CDS             complement(3367..4335)
                     /locus_tag="ACZPD3_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR1414582.1"
                     /translation="MKSSNIGGQAVMEGIMMRHKDKYSIAVRRPDNEIELKVEDYKCV
                     FGNAKFLKYPLIRGVVSFVDSLVVGTKCLMYSAEIAGDEEDEEDKQKNAALSEEELAA
                     KKAKEDKQFKWLLYVTVAVSIVVSVAAFMLLPYALASLCRRVGASEFAVTIVEAFVKL
                     ALFMGYMLLISRMKDIQRTFMYHGAEHKCINCVEHGLPLTVDNVLASSRLHKRCGTSF
                     LFLVMLVSIFLHFIFVLVPFYWVRLFGRLLMVPVVAGISFEIIQWAGRSDSKLADFFS
                     KPGLAMQKLTTKEPTADMAEVAIRAVEAVFDWKAYLKEEFGVEAEQ"
     gene            complement(4410..4613)
                     /gene="rpmE"
                     /locus_tag="ACZPD3_00755"
     CDS             complement(4410..4613)
                     /gene="rpmE"
                     /locus_tag="ACZPD3_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR1414583.1"
                     /translation="MREGIHPNYYQATVTCNCGNTFVTGSTKQDIHVEICSKCHPFYT
                     GQQKAAQARGRIDKFNKKYGLNK"
     gene            complement(4802..6199)
                     /gene="rho"
                     /locus_tag="ACZPD3_00760"
     CDS             complement(4802..6199)
                     /gene="rho"
                     /locus_tag="ACZPD3_00760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR1414584.1"
                     /translation="MREKYESLSLATLKDLAKARGLKGVSALRKPELIERMLHEDELE
                     GETKKQEAVREKPQQSEVEGNGERIPQYNKTPERTQYHAPAEAVQLDSGERADGILEV
                     LPDGYGFIRCENYLPGENDIYVSPSQIRRFNLKTGDIIKGNIRIKTQGEKFSALLYVT
                     SINGFHPSEGQRRYNFEDMTPIFPNERLIMERPGGTVAMRIVDLISPIGKGQRGMIVS
                     PPKAGKTTLLKDVAKSILRNNPDMHLIILLIDERPEEVTDIREAICGDNVEVIYSTFD
                     ELPEHHKRVSEMVIERARRLVEHKKDVTILLDSITRLARAYNLIIPPSGRTLSGGLDP
                     AALHMPKRFFGAARNMREGGSLTILATALVDTGSKMDDVIYEEFKGTGNMELVLDRKL
                     QEKRVFPAIDIQKSGTRREDLLLSKEEQEAVYIMRKALNGMKSEDAVEQILNMFTRTK
                     NNAEFVQTVKKQKFI"
     gene            complement(6638..7138)
                     /locus_tag="ACZPD3_00765"
     CDS             complement(6638..7138)
                     /locus_tag="ACZPD3_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR1414585.1"
                     /translation="MEALVHWFSQNLSQYISPEGAVFIISMIPLLELRGGLLAASLLK
                     ISAAKAIPLCIVGNIIPIPFILLFIRQIFKVLKKTKLFRGLVMKLEDRAMRKSDQVKK
                     YEFWGLMLFVGIPLPGTGAWTGSLIASLLEIDIKKSSLAIFCGIIMATVIMYIVSYGL
                     VGNLVH"
     gene            complement(7154..8209)
                     /gene="hisC"
                     /locus_tag="ACZPD3_00770"
     CDS             complement(7154..8209)
                     /gene="hisC"
                     /locus_tag="ACZPD3_00770"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR1414586.1"
                     /translation="MSRWEEDIRKVIPYTPGEQPNQPDMIKLNTNENPYPPAPGVEKA
                     LREMDTDTMRLYPDPTAGELVHAIAKNYRLKDEQVFVGVGSDDVLAMSFLTFFNSQKP
                     VLFPDITYSFYDVWADLFRIPYERPALDENFHIRKEDYFRENGGIVFPNPNAPTGVEM
                     PLEEVEDIIRHNPDVIVIVDEAYVDFGGQSALPLIEKYDNLLVVQTFSKSRSMAGMRI
                     GFACGNEKLIRFLNDVKYSFNSYTMDRTAIAAGVAAVEDKAYFDETCNKIIETREWTK
                     KELKALGFSFQDSMSNFIFATHKICPAKELFEALRGQHIYVRYFQKDRIDNFLRITVG
                     TKEEMQKFINFLKDYLK"
     misc_binding    8357..8584
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8749..10710
                     /gene="thrS"
                     /locus_tag="ACZPD3_00775"
     CDS             8749..10710
                     /gene="thrS"
                     /locus_tag="ACZPD3_00775"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR1414587.1"
                     /translation="MIVTLKDGSKKEYAEAKSVIDIAYDISEGLARAACAGEVNGEVV
                     DLRTVIDSDCELNILTAKDEKGLAVLRHTASHVLAQAVQTLFPDAKVAIGPSIDTGFY
                     YDFDCPPFSRDDLDAIEKEMKKIIKKGAKIERFTKSREDAIAYFQEKNEPYKVELIED
                     LPEGSEISFYSQGDWTDLCAGPHLMSVKGVKAFKLLSSSSAYWRGSEKNAMLTRIYGT
                     AYASKDELKEHLEQMEEAKKRDHNKLGREMKIFTTVDVIGQGLPLIMPNGVIMMQELQ
                     RWIEDEETKRGYIRTKTPLMAKSDLYKISGHWDHYKEGMFVLGDEETDKEVFALRPMT
                     CPFQYYVYKAEQHSYRDLPLRYGETSTLFRNEDSGEMHGLTRVRQFTISEGHLIVRPD
                     QMVKEFKDCIALAQYCLQVLGVEEDVTYHLSKWDPNNREKYIGDAEVWNQTEAHIRQM
                     LEELNIPFTEDVGEAAFYGPKVDINAKNVYGKEDTMITIQWDALLAEQFDMYYIDENG
                     EKQRPYIIHRTSMGCYERTLAWLIEKYAGMFPTWLCPEQVRVIPISEKFHDYAAKVEA
                     QLKENGIRCSVDQRSEKMGYKIREARLARVPYMLIVGAKEEEEGKVSVRSRYLGDEGM
                     KDLGDFLAAIKEEIKNKTIRKIEVQEENK"
     gene            complement(10807..>13105)
                     /locus_tag="ACZPD3_00780"
     CDS             complement(10807..>13105)
                     /locus_tag="ACZPD3_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR1414588.1"
                     /translation="YKDAKLLRITRYRYNNIPADSNGKYYYINDDGVIWNPGTMPSAT
                     ETDTYECRHGLGYSRFCSSKNNLKAELLVFVPSDASCEINCLKLKNNSETEKNISLFS
                     YVEFCLWNAVDDASNFQRNLSIGEVEVQGSTIYHKTEYRERRKHYSFFTVNTQVDNYD
                     TNRDAFLGAGNGNAFPEAVCKKKCSNSIASGWYPIAAHQIDLTLLPGEEKEFVFMLGY
                     CENPADDKWEAPGIINKTSAKALIERFNTMEKAMQAFAELKNYWETLLARFAVVSENE
                     HIDRMANIWNQYQCMVTFNMSRSASYYESGTGRGMGFRDSCQDLLGFVHLIPERARER
                     IIDIASTQFPDGSAYHQYQPLTKQGNLDVGSGFNDDPLWLIAAVAAYIKETGDYGILD
                     VQVPFDCKKGTEVPLFEHLEKSFYYTVTHLGPHKLPLIGRADWNDCLNLNCFSSEPGE
                     SFQTTGPSEGPVAESVFIAGMFVKYGKEFEELCERTGHTELAKEAGKAVDEMYQAVLD
                     NGWDGEWFLRAYDAQSEKVGSKECEEGKIFIEPQGFCVMAGIGKEEGIAEKALDSVNE
                     LLETKYGIMILQPAYTRYHLELGEITSYPPGYKENAGIFCHNNPWISIAETVLGRGNR
                     AFEVYRKICPSYIEEISDIHRTEPYVYSQMIAGADAPGFGEAKNSWLTGTAAWTFINL
                     SQALLGILPGYDGLIVDPCLPETFGDFKVNRHFRGADYHIEIHKPKGVQKGVRWIEVD
                     GERINGNQIPMVNGRNEYHVIVQMG"
BASE COUNT         3607 a         3219 c         2300 g         3979 t
ORIGIN      
        1 tctgcaccgg acgctgtcag acctgcaaca agtgtatatt caaacatata actgcttctt
       61 cttggatctt ttcaaataac aatcttagct ttatggtctc ttccataata ccagcctacg
      121 tagcgcccca ccttaaatgc atgatctact gtcagtttaa catttgcttc tccacgaaat
      181 ccatctgttc caaaatattt tcccataatt gctctccttt actttttaca atatcctctg
      241 tttctgcact gcatactgta tttcccggat cacacccaaa actgctttta atcagatcag
      301 acatgtctcc tgcgaaagct gcagatatct gcttaactta ttgataaagt ccctgaggat
      361 atatacccgc ttctgtaaga tttttaaatg ctctcattac tgtttcttta tcttcctgat
      421 aggttacacc aaaccatgta tcctttgtct ccaggacatc aacttcagca ctttcatttt
      481 tgatcagatg gtcaactaac tccggaagca gatattcctt ttttgtatct tccggtgcga
      541 gtccactgag aaattctgaa aatcctttct caagaatctc cataaagtcc ggtgtcagtc
      601 cccacatatt catggacacc agatccttta catccagttc ctgcacactt ccgtctgcat
      661 ttcttgactc tgcacctgtg gcagtcttaa atatatcatg tgtctctgtc acacctgtaa
      721 gctgtccatt cttaatatga cagattcctc tggtaacact tccattatcg ctcaatgtat
      781 ttccaagcct gaagccggcc atacagatat ggagttttcc gtcctttggc tgttcctgca
      841 caagataatc atgcaccttc acataagctt cttttccata ataatcatct gcattgataa
      901 caataaatgg ctcatccaga accttctttg cagcaagtac agcctgtccg gttccccagg
      961 gcttcgtgcg gtcagccggt ttcgtaaatc cctccggcag atcatcaagc tcctggaata
     1021 catattctac ttccgtaatt ttctcaatac gctcgccgat cactctacga aactcttctt
     1081 ccagatcttt acggataata aacactactt tgttaaatcc ggcttccagc gcatcgtgga
     1141 tggaataatc cataataatc tctccacatg gtcccaccgg tgcaagctgc ttgattcctt
     1201 ttccaaaacg acttccgatt cctgccgcca taattacaag tgctgttttc tgcataaata
     1261 ttttcctcct gacattcttc tctcatatca atactttttc agtcatcttc tgtcacatac
     1321 catctccgca atattctatt ccgcatcctg aagattactt aattttgctg cctgatctgc
     1381 tgcgatcaga ctgtcaatga tctcatccag atccccgttc agaacattct ccagtctgtg
     1441 cagtgtcagt ttgattctgt ggtctgtcac acgtccctgt ggaaaattat aagttctgat
     1501 tttctctgaa cggtcaccag ttccaatctg gcttcttctt gcctctgcct cagcatcatg
     1561 ctgtttggca agctccatct catacagacg ggagcgaaga actttcagcg ctttatcttt
     1621 attcttaagc tgtgacttct catcctggca ggaaattacg attccggtag gaatatgtgt
     1681 cagtcgcaca gcagagtctg ttgtattgac acactgtccg ccgtttccgg atgcacggaa
     1741 cacatcgaat ttacagtcat tcatatccag atggaaatcg atttcctctg cttccggcat
     1801 aatagcaact gtacaggtag aggtatggat acgtccgccg gattctgttt ccggaactcg
     1861 ctgtacacga tgtacaccac tttcatattt cagtctggaa taagcacctg cacctgtgat
     1921 cataaatgtt acttccttaa atccaccaat tccgttctca ttgagactca tcatttctgt
     1981 tttccagcgg cgggattctg catatttcac atacatacgg taaatctctg ctgcaaaaag
     2041 ggcagcttca tccccacctg cacctgcacg gatctccaca ataacgttct tgttatcatt
     2101 aggatctttc ggaagaagaa ggatcttaag ttcatgctcc agttcctcta cattcttctt
     2161 tgcctcagac agttcttcct tcagcatttc ccgcatatct tcatctttct cttcctcaag
     2221 catggaaaga ctgtcctgta tcgtttcttt gtttttctta tactcccggt aagcatctac
     2281 tatcggctgc agatcgctct gctctttcat cagcttctga aaatgttcct gattatcggt
     2341 aactcccggc tctccaagct cattaagcac ttcttcaaaa cgaatgagca gatcgtctaa
     2401 tttatcaaac atatttatcc tcctgtaatt tctttcgccc cagaaccacc cggtccaacc
     2461 cggccagatc ctgccttatc tcaatatctt caaatttata tttccgcaaa agagctgcca
     2521 catcttctcc ctggctgcat ccgatttcat acaaaagcca gccacccgga acaagatgat
     2581 cctgtgcctg tttcgtgata gccctgtaaa aataaagtcc atcttccata ccatccagcg
     2641 ccatacgcgg atcatgcaat tttacttcat ccataagatc ttcaatttcc gctgtaggaa
     2701 tatatggcgg atttgatata atcatatcat aagctctgct ataatttcta ttactatttt
     2761 cgcattctgt catttttaca gtatctttta cagcagtcct tttcatatac tctgttccgt
     2821 acacaatgtt ctgcatttca gaaaacagat tactctgtac gaattccgca tgctctgcca
     2881 cttccagtgt ccgtgcattc cgcacagcca ctttcagtgc ctcatctgac agatccacgc
     2941 ctgttccgtg tgcatcagct ctctccttca gaatactgat aagaatacag ccggatcctg
     3001 tacacatatc aagaatatat ggatttttca cagttttcat gcattcaaga gcagactcca
     3061 caagtgtttc cgtatcctgt ctgggtacca gtacattctt atccacataa aattcatgcc
     3121 ccataaaaaa agcctgatgc gtcagatgct gcagcggtat gtgtcccgca cgtctgctga
     3181 tcagttccag atatctttca gcaacacttt ctgtgacact ttcttctccg tatgcaaaat
     3241 aatacgcccg gctttttcct gtgacatatt ccaggagaag ccaggcatcc agcccggctt
     3301 ccttaatccc cgcctgtgca aggatcatct gtcctttttt taataatcct gtcagagttt
     3361 ctccgttcat tgttctgctt caactccaaa ttcttccttc agatatgctt tccagtcaaa
     3421 aaccgcctct acagccctga tcgcaacctc tgccatatct gctgtcggtt ccttagttgt
     3481 aagtttctgc attgccagac ccggtttact gaaaaagtca gcaagtttgc tgtctgatct
     3541 tccagcccac tgaataatct caaaagatat tcctgctaca accggcacca taagaagtct
     3601 tccaaacagt ctcacccagt aaaatggaac caatacaaaa ataaaatgca gaaaaatgct
     3661 gaccagcata accagaaaca gaaaactggt tccacatctc ttatgcagtc tggagcttgc
     3721 aagaacatta tccaccgtaa gcggcagtcc atgttccacg cagttaatgc acttatgctc
     3781 tgctccatga tacataaatg ttctctgaat atccttcatt ctggaaatca gaagcatata
     3841 tcccataaac aatgcaagct ttacaaatgc ctcaactatt gtaactgcaa attccgaagc
     3901 gccaaccctt ctgcaaagac ttgcaagggc gtatggcagc aacataaagg cggcaactga
     3961 taccacgatc gagacagcaa ctgtcacata taacagccat ttgaactgct tatcttcttt
     4021 tgcctttttt gcagcaagct cctcttctga aagagcagca ttcttctgct tatcctcttc
     4081 gtcctcctca tcccctgcaa tctccgctga atacatcaga cattttgtgc cgaccaccag
     4141 agaatccacg aagcttacca cacctcggat cagtggatat ttgagaaatt ttgcatttcc
     4201 gaatacacat ttgtaatctt ctacttttaa ttcaatctca ttgtccggtc tgcggacagc
     4261 aatggaatat ttatccttat ggcgcatcat aatgccttcc attacagcct gaccgccgat
     4321 gttcgatgat ttcattatat ttctcccaaa gaaacaagaa aggctgagat ttcccaacct
     4381 caaccttctc aattcttact atattctgat tatttgttca ggccgtattt cttattaaac
     4441 ttatcgatac gtccacgagc ctgagctgct ttctgctgac ctgtatagaa tggatgacat
     4501 ttagagcaga tttctacgtg gatgtcctgt tttgtagatc ctgttacgaa tgtgttacca
     4561 cagttgcagg ttacagttgc ctgataataa ttaggatgga ttccttctct catgattttc
     4621 acctctctgt aaagattcta ttttctattc gtattcattt gacgaattta ctattaaaca
     4681 gctttatgag tataacatat cgtcccatgg aatgcaagtt attttaaaat ttattttaaa
     4741 atttatttaa tatttttgtt actgagaacg gagcgaacag taactgtttt tttacaacgt
     4801 ttcagataaa cttctgtttt tttacagtct gcacaaattc agcattattt ttcgtacggg
     4861 taaacatatt caggatctgt tctaccgcat cttctgactt cattccattg agggcttttc
     4921 gcataatgta aaccgcttcc tgttcttctt tactcagcag cagatcctct cttcttgtac
     4981 cggatttctg gatatcaatt gcagggaata cccgcttttc ctgcagtttt cggtcaagca
     5041 ccagttccat attaccggtt cccttaaatt cttcataaat tacatcatcc attttacttc
     5101 ccgtatccac aagcgctgta gcaagaatag taaggcttcc gccctctctc atatttctgg
     5161 ccgcaccaaa gaaacgtttc ggcatatgaa gagccgccgg atccagacca ccggaaagag
     5221 ttcgtccgct gggcggtatg atcaggttgt atgcccttgc cagtcttgta atagaatcaa
     5281 gaagaattgt aacgtctttt ttatgttcca ccagacgacg tgcacgttct attaccattt
     5341 cagacacacg tttatgatgt tccggaagct catcaaatgt agaataaatg acttccacat
     5401 tatcgccaca gatggcctcc ctgatatctg taacctcctc agggcgctca tcgatcagaa
     5461 gaatgatcag atgcatgtcc ggattgtttc tcagaataga ctttgccaca tctttcagca
     5521 aagttgtctt accggctttc ggaggagata cgatcattcc tctctgtcct ttaccgatag
     5581 ggctgatcag atctacgatc ctcatggcaa ctgtgccgcc cggtctttcc attatcagtc
     5641 tctcatttgg aaaaatcggt gtcatatctt caaaattata tcgtctctgc ccttcactgg
     5701 gatggaatcc gttgatagag gtcacatata gcagggcact gaatttctct ccctgtgttt
     5761 tgatacggat attcccctta ataatatcac ctgttttcag gttaaatctg cgtatctgag
     5821 aaggtgatac ataaatatca ttttcccccg gaagataatt ttcacatcgg ataaacccat
     5881 atccatccgg aagtacctca aggattccgt ctgctctctc tccactgtca agctgtactg
     5941 cctctgccgg ggcatgatac tgtgtacgtt ccggtgtttt gttatactgg gggattctct
     6001 ctccgttacc ttccacttca ctctgctgcg gtttctcccg gactgcttcc tgtttttttg
     6061 tctcaccttc cagttcatct tcgtgaagca ttctctcgat caattccggt tttctcaatg
     6121 ctgaaacccc ttttaatcct ctggcttttg ccaaatcctt taaagttgcc agtgataatg
     6181 attcatactt ttctctcata aaataatcct ctgctttttg ttttgattgt taatattgat
     6241 aattattttt atttttgttt ttctgatttc ctatgtatct gagtaattta ttctttatta
     6301 acttttcctg taacagttag tgggataatc tgtctgaagt gacacgaata tcagatctgc
     6361 agcaatgcat atcgcaggaa aattatactc ctgctcctgt gtcgacaagt taattcctgt
     6421 agtaatcctg tctgcacacc ggtatcttta tatttatctt agcacatctt cttcataatt
     6481 gacaatacca tattattcca aatctttatt attggcaagc tgttttatac gtcataaaac
     6541 tgatattatc gacataaaag cagaaatccg gagacataaa atatgtcccc ggatttccgg
     6601 taaatcagat attaataaca cttatttaag aagacaatta atgtaccaga tttcccacta
     6661 atccataaga aacaatatac ataatgacag ttgccataat aatgccacaa aaaattgcca
     6721 gagaggactt cttaatatca atttccagaa gagatgcaat cagagatcct gtccatgccc
     6781 ctgttcccgg aagcggaatt cccacaaaca gcatcagtcc ccaaaactca tatttcttta
     6841 cctgatcact ttttctcatt gccctgtctt ccaatttcat aacaagtcct cgaaagagtt
     6901 tcgtcttttt taaaaccttg aaaatctgtc tgataaacaa cagaataaac ggaataggaa
     6961 taatatttcc aacaatacac agtggaattg cctttgcagc cgatatctta agtaaggatg
     7021 ctgcaagcaa tcctcccctg agttccaaaa gcggaatcat tgaaataata aaaactgccc
     7081 cttccggtga aatatattgt gatagattct gcgagaacca atgtaccagt gcttccataa
     7141 aacctcctaa cttttacttc agataatcct tcaaaaaatt aataaatttc tgcatttctt
     7201 cctttgttcc aactgtgata cgaaggaaat tgtcaattct gtccttctgg aagtaacgaa
     7261 cataaatatg ctgccctctc aatgcctcaa aaagttcttt cgcagggcag attttatgcg
     7321 tagcaaaaat aaagttagac atagaatcct gaaaagagaa tcccagtgcc ttcaattctt
     7381 tctttgtcca ttctcttgtc tctatgattt tattacatgt ctcatcaaaa tatgctttat
     7441 cttcaactgc cgccacacct gcagcaatcg ctgtacggtc catagtataa gaattaaaag
     7501 aatacttcac atcattcagg aatctgatca gtttctcatt tccacacgca aaaccaattc
     7561 tcatcccagc catagaacgc gacttggaaa atgtctgtac gaccagaagg ttgtcatatt
     7621 tttcaatcaa tggaagcgcc gactgaccac caaaatccac ataagcctcg tccacgatca
     7681 caatcacatc aggattatgg cggataatat cctccacttc ctccagcggc atctccacac
     7741 ctgtcggcgc attcggattc gggaatacaa ttcctccgtt ttctctgaaa taatcttcct
     7801 tccttatatg aaagttctca tccagtgccg gtctctcata tggaatgcga aacaggtctg
     7861 cccatacatc ataaaaagaa tatgtgatat ccggaaaaag aaccggcttc tgtgaattaa
     7921 agaatgtaag gaaactcatg gcaagcacat catcggaacc tactcccaca aaaacctgtt
     7981 catccttcag cctgtaattc tttgcaatgg catgtaccag ttctcctgct gtaggatccg
     8041 gataaagtct catagtatct gtatccatct ccctcaacgc cttctccact ccaggtgccg
     8101 gcggatacgg attctcgttg gtattcagct tgatcatatc cggctgattg ggctgctcgc
     8161 ctggtgtata tggtattact ttacgtatat cttcttccca tctgctcatt tatctgattc
     8221 ctttcatgtt ttttattgtt tttcactttc gtcagtataa cattaatctc tattttcgta
     8281 aatcaacttt ttgcatttta ctgattgaca aacccacttt gcaagtttat aataggcggc
     8341 agaaaagata agaaggatca aaacgttgaa gagaagagta cgctgaggaa cgttacagag
     8401 agaaatgtat accggtgcaa gcatttcatc gcgaaccagc cgaagaccac ctctgagtga
     8461 ctttaataca gtccggtgat tccgttacca tcgaatgaag cggttctgat cagatgattt
     8521 atccatcagt aatgtatcag aacaaccggg gtggaaccgc gagtaatgtt atctcgtccc
     8581 ctacagtatt taaccgtact gtaggggatt tttttattct ttcttttacc ccttcgcagt
     8641 acaaaaaact gttactgttc actccgttct cagcaacacg ctccgcagga tatcaacagt
     8701 gaacagcaac tataacaaat ccatactaac gtaaaggaga taatcatcat gatcgtaaca
     8761 ttaaaagacg gctcaaaaaa agaatacgct gaagcaaaat ccgtaatcga cattgcttac
     8821 gacatcagcg agggacttgc acgtgctgcc tgtgcaggag aagtaaacgg agaagttgta
     8881 gacctgagaa cagttattga cagtgactgc gaattaaaca tcctgacagc aaaagatgaa
     8941 aaaggacttg cagttctccg ccatacagca tcccacgtac ttgcccaggc agtacagacc
     9001 ttattcccgg atgcaaaggt tgctatcgga ccttctattg atacaggatt ctactatgat
     9061 ttcgactgcc ctccattctc ccgtgatgac ctggatgcaa tcgaaaaaga aatgaaaaag
     9121 atcatcaaaa aaggcgctaa gattgaacgc ttcacaaaat caagagaaga tgccatcgca
     9181 tacttccagg agaaaaatga accatacaag gtagaactta tcgaagatct tccggaaggc
     9241 tccgaaatct ctttctattc ccagggagac tggacagacc tctgcgcagg tcctcacctt
     9301 atgagtgtaa agggtgtaaa agcattcaag cttctctcct cttccagcgc ttactggaga
     9361 ggcagcgaaa agaacgcaat gctcacacgt atctacggaa cagcttatgc ttccaaggat
     9421 gagttaaaag aacatctgga acagatggaa gaagcaaaaa aacgtgacca caacaaactt
     9481 ggccgtgaga tgaagatctt cacaactgta gacgtgatcg gtcagggact tccacttatc
     9541 atgccaaacg gtgtgatcat gatgcaggag cttcagcgct ggattgaaga cgaagaaaca
     9601 aaacgcggat atatcagaac aaaaactccg ttaatggcaa agagcgacct ctacaagatt
     9661 tccggacact gggatcatta caaagagggt atgttcgttc tcggcgacga agaaacagac
     9721 aaagaagtat ttgcgcttcg tcctatgaca tgcccattcc agtactatgt atacaaagca
     9781 gaacagcaca gctatcgtga tctgcctctt cgttacggtg aaacatccac tctgttccgt
     9841 aatgaggatt ccggagaaat gcatggtctg acacgtgtac gtcagttcac gatttccgaa
     9901 ggtcatctga tcgtaagacc tgaccagatg gtaaaagaat ttaaagactg tatcgcactt
     9961 gcacagtact gcctgcaggt tcttggtgta gaagaggatg taacctatca cctttctaaa
    10021 tgggatccga acaacagaga gaaatacatc ggtgatgcag aagtgtggaa tcagacagaa
    10081 gctcatatcc gccagatgct tgaagaactg aacattccat ttacagagga tgttggagaa
    10141 gctgcattct acggacctaa ggttgatatc aatgccaaaa atgtatacgg aaaagaagat
    10201 acaatgatca cgatccagtg ggatgctctt cttgctgaac agtttgatat gtactatatc
    10261 gacgaaaatg gcgaaaaaca gcgtccatac atcatccacc gtacatccat gggatgctat
    10321 gagagaacac tggcatggct gattgagaaa tatgcaggta tgttcccgac ctggctctgc
    10381 ccggaacagg ttcgtgtaat tccgatctct gagaaattcc acgactatgc tgccaaagtg
    10441 gaagcacagt taaaagaaaa cggaatccgc tgctccgttg accagcgttc cgagaaaatg
    10501 ggctacaaga ttcgtgaagc acgtcttgca agagttccat acatgctcat tgttggcgcc
    10561 aaagaggaag aagaaggcaa ggtttccgta agaagccgtt accttggtga tgaaggaatg
    10621 aaagaccttg gtgatttcct tgcagcgatc aaagaagaaa tcaagaataa aacaatccgc
    10681 aaaatcgaag tacaggaaga aaataaataa aacaggtatt ttactttaac ctgttcaaat
    10741 aagtccccac ccactgcttt aattgcatca ggcaataagc aatgggtggg gacttttatt
    10801 tatcagttat cccatctgta caattacatg gtattcgttt ctgccattga ccataggaat
    10861 ctggtttcca tttatcctct caccatctac ctcaatccat ctgactcctt tctgcacacc
    10921 tttcggttta tggatctcaa tatgataatc cgcccctcta aaatgacggt tcactttaaa
    10981 atcgccaaac gtttccggaa ggcaggggtc tacgatcaaa ccatcatatc ctggcagaat
    11041 accaagaagc gcctgtgaca ggtttataaa tgtccacgct gcagttcctg tcagccaact
    11101 gttttttgcc tctccaaatc ctggtgcatc agctcccgcg atcatctgcg aatatacata
    11161 tggttctgtc cggtgaatat cactgatttc ctcaatataa gatggacata ttttacgata
    11221 aacctcaaat gccctgtttc ctcttccaag cacagtctcc gcaatggaaa tccacggatt
    11281 gttgtggcag aaaattcctg cattttcttt atatccaggc ggataagaag taatttcccc
    11341 aagttccaga tggtatcttg tataagccgg ctgaaggatc attattccat actttgtttc
    11401 cagcaattca ttcacagagt ccagagcttt ttctgctatt ccttcttcct tgccgattcc
    11461 tgccataaca cagaatccct gtggttcaat aaaaatcttt ccttcttcgc attcttttga
    11521 accaaccttt tcagactgtg catcataggc acgcaggaac cattctccgt cccatccatt
    11581 atccagcact gcctgataca tctcatccac tgctttccct gcttcttttg caagttctgt
    11641 gtgcccggtc cgctcacaga gctcctcaaa ttcttttcca tatttcacaa acatacctgc
    11701 aataaataca gattctgcca cagggccttc cgacggccct gttgtctgaa aggattctcc
    11761 aggttcactg gaaaaacagt tcagattcag gcagtcattc cagtctgccc ttccgatcag
    11821 aggaagtttg tgaggaccaa gatgggttac tgtgtaataa aaagatttct ccagatgttc
    11881 aaaaagcggt acctctgttc ccttcttaca atcaaagggt acctgcacat caagtatccc
    11941 ataatcacca gtctctttga tatatgctgc tactgctgcg atcagccata acgggtcgtc
    12001 attaaatccg cttccaacat ccagattccc ctgttttgtc agcggctggt actgatgata
    12061 tgcacttcca tccggaaact gtgtagatgc tatatcaata atcctttccc ttgcccgctc
    12121 cgggataaga tgcacaaatc ccagaagatc ctgacaagaa tcgcgaaacc ccatcccccg
    12181 tcctgttcct gattcgtaat aagatgctga tctggacata ttgaaggtta ccatacactg
    12241 atattgattc cagatatttg ccatgcggtc aatatgttcg ttctcgctga cgactgcaaa
    12301 gcgtgccagc aatgtttccc agtaattttt cagttctgca aaggcctgca tcgctttttc
    12361 cattgtatta aatctttcaa tcaatgcttt tgcagaggtt ttattgataa ttccgggagc
    12421 ttcccattta tcatctgccg gattttcaca atatcccagc ataaaaacaa attctttttc
    12481 ctctcccggc agcagagtca gatctatctg atgtgcagca atgggatacc atccgcttgc
    12541 aatagagtta ctgcattttt tcttacatac agcttctgga aaagcattcc cgtttcctgc
    12601 tcccagaaaa gcatctctgt tggtatcata attgtccacc tgtgtattta ctgtaaagaa
    12661 actgtaatgt ttccttcttt ctctgtattc tgtcttatga taaatcgtac tcccctgtac
    12721 ttccacttct ccgatactta aatttctctg gaagttcgat gcgtcatcca ctgcattcca
    12781 gaggcagaat tccacgtatg agaacagaga aatatttttt tcagtttcag aattattttt
    12841 cagtttcaga caattgatct cacatgaagc atcagagggc acaaaaacca gaagctctgc
    12901 ttttagatta tttttcgaag aacagaagcg actgtatcca agaccatgtc tgcattcata
    12961 agtatctgtt tctgttgcag acggcatagt tcccggattc cagattactc cgtcatcatt
    13021 aatataataa tactttccgt tggaatctgc aggaatatta ttataacggt acctggtaat
    13081 tcgcaacaat tttgcatctt tataa
//
LOCUS       DBMDXG010000010        12523 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1455_length_12523_cov_1.889229, whole genome shotgun sequence.
ACCESSION   DBMDXG010000010 DBMDXG010000000
VERSION     DBMDXG010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 12523)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12523)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12523
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1455_length_12523_cov_1.889229"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            287..2095
                     /gene="lepA"
                     /locus_tag="ACZPD3_00785"
     CDS             287..2095
                     /gene="lepA"
                     /locus_tag="ACZPD3_00785"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR1414589.1"
                     /translation="MIDQSKIRNFCIIAHIDHGKSTLADRIIEMTGTLTEREMQSQVL
                     DNMELERERGITIKSQAVRIVYKAKDGEEYIFNLIDTPGHVDFNYEVSRSLAACDGAI
                     LVVDAAQGIEAQTLANVYLALDHDLDVLPVINKIDLPSAEPDRVVNEIEDVIGLEAHD
                     APRISAKTGLNVEEVLEQIVTKIPAPHGDVDAPLKALIFDSIYDAYKGVIVFCRVMDG
                     RVKRGTQIHMMATGFTTEVVEVGYFGAGQFIPCEELTAGMVGYITASIKNLGDTRVGD
                     TVTDKNRPCAEALPGYKKVNPMVYCGLYPADGAKYGDLRDALEKLQLNDASLFYEPET
                     SVALGFGFRCGFLGLLHLEIIQERLEREYNLDLVTTAPGVIYKVYKTNGEVINLTNPS
                     NLPDPSEIEYMEEPMVNAEIMVTTEFIGAIMDLCQERRGQYLGMDYMEETRALLKYKL
                     PLNEIIYDFFDALKSRSRGYASLDYELCGYERSELVKLDILVNKEEVDALSFIVHADT
                     AYERGRKMCEKLKDEIPRQLFEIPIQAAVGSKIIARETVRAMRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQVGNVEIPQKAFMSVLKLDDK"
     gene            2197..2553
                     /locus_tag="ACZPD3_00790"
     CDS             2197..2553
                     /locus_tag="ACZPD3_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414590.1"
                     /translation="MKKMIKRKTVILCMLLLGISVSGCGKTRKVDEASSKVIQISVAP
                     EETSPTPAPDQVASAAVTTNGNLTMVNTYLAEQDAAGISSAESSDSQSDADSTDNSVD
                     SADNNEQDSSTSDTEE"
     gene            2583..3725
                     /gene="hemW"
                     /locus_tag="ACZPD3_00795"
     CDS             2583..3725
                     /gene="hemW"
                     /locus_tag="ACZPD3_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR1414591.1"
                     /translation="MERRNITDSPMEIYVHIPFCIKKCDYCDFLSGPSGPKEQADYVD
                     ALLEEINAAEEGKGRSVSSVFIGGGTPSVLDERFIGEILNHIRRKFQIADHAEITIEV
                     NPGTADRNKLQAYRTYGINRLSIGLQSPDDRELKILGRIHNYEQFLETYRSAREAGFD
                     NINIDLMSAIPDQTYEGWIHNLRTVAGLDPEHISAYSLIIEEGTPFASRTLNLPDEDA
                     EYNMYEATAQILREYGFEQYEISNYAKKGRECRHNVGYWIRQDYLGFGLGASSLYGKE
                     RFANTQDMKKYLENSRTPEKIREKEPPLTREDEMAEFMFLGLRMTRGISKAEFERQFG
                     SEIDAIYGDVLRKYKSMGLLLEENGRIFLSREGIHVSNSVMADFLP"
     gene            3886..6726
                     /gene="uvrA"
                     /locus_tag="ACZPD3_00800"
     CDS             3886..6726
                     /gene="uvrA"
                     /locus_tag="ACZPD3_00800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR1414592.1"
                     /translation="MAADTTKKHFIRIRGANVNNLKNLSVDIPRDKFVVFTGLSGSGK
                     SSLAFDTIYAEGQRRYMESLSSYARQFLGQMEKPDVESIEGLPPAISIDQKSTNRNPR
                     STVGTVTEIYDYFRLLYARVGIPHCPKCGREIKKQTVDQMVDSIMSFPERTKIQLLAP
                     VVRGRKGTHAKLLEQAKRSGYVRVQIDGNLYELSEEISLDKNIKHNIEIVVDRLIVKP
                     GIEKRLSDSIETVLNLAEGLLMVDTMDGNIHNFSQSFSCPDCGISVDEIEPRSFSFNN
                     PFGACPDCLGLGYKMEFDIDLMIPDRSLSILDGAIVVTGWQSCTNEGSFSRAILDALA
                     REYDFSLATPFEEYPEKIQDILINGTNGHSVKVYYKGQRGEGVYDVAFPGLIRNVEQR
                     YRETGSDAMKQEYESFMRITPCKTCKGQRLKKESLAVTVADKNIYEVTNLSIEKLKAF
                     LADMQLSEQQQLIGRQILKEIRARVSFLSDVGLDYLSLGRATGTLSGGEAQRIRLATQ
                     IGSGLVGVAYILDEPSIGLHQRDNDRLLGSLMKLRDLGNSLIVVEHDEDTMRAADCIV
                     DIGPGAGEHGGQLVAMGTAEDLMKNEDSITGAYLSGKLKIPVPLERRKPTGFLTVKGA
                     AENNLKNIDVKIPLGIMTCITGVSGSGKSSLINEILYKRLARDLNRARVIPGKHKDIL
                     GTDQLDKVINIDQSPIGRTPRSNPATYTGVFDQIRDLFAATADAKAKGYKKGRFSFNV
                     KGGRCEACSGDGIIKIEMHFLPDVYVPCEVCKGKRYNRETLEVKYKDKNIYDVLNMTV
                     EEALTFFENVPSIKRKIQTLYDVGLSYIRLGQPSTELSGGEAQRIKLATELSKRSTGK
                     TIYILDEPTTGLHFADVHKLVEILKRLSEGGNTVVVIEHNLDVIKTADYIIDIGPEGG
                     DKGGTVVAQGTPEEVAQSPVSYTGKYVKKYLK"
     gene            6980..7969
                     /gene="mreB"
                     /locus_tag="ACZPD3_00805"
     CDS             6980..7969
                     /gene="mreB"
                     /locus_tag="ACZPD3_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR1414593.1"
                     /translation="MPTSDIGIDLGTRNSLAYSTGKGLVLNEPSIVVYDKNTEKIRAI
                     GEEARLMEGRITSDMEIIRPIRQGVIVDYTVTEKMLKYFISRAIGRRAFRKPRISICV
                     PSGITEIEKKAVEEATYQAGARDVYMVEEPIAAAIGAGVDVTKPFGNLVVDIGAGTSD
                     VAVISLAGVVVSASVKVAGDTFNQAILNYVRKNHGLFIGEDMAEKIKIQIGTAIEESN
                     PRTMEVKGRNVITGLPKTVTLTSEEIRVALKDATSQIVETVHGILEKTPPELAADIVD
                     RGIVLTGGGALLHGMDTLIEQRTGVSTLTVQDAMSVVAVGTGKYAEVMARFDG"
     gene            8048..10312
                     /gene="recD2"
                     /locus_tag="ACZPD3_00810"
     CDS             8048..10312
                     /gene="recD2"
                     /locus_tag="ACZPD3_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR1414594.1"
                     /translation="MSETVTGYIDHVIFRNEDNGYTVMVLKGTKKEEELTCVGSFPAI
                     TQGASIEATGVYIHHPVYGKQFQISSFTEKMPEDTYGIERYLGSGAIRGIGAALAARI
                     VRKFGDDTLRIVEEEPERLAEVKGISEKKAREIAAQVSEKAEMRKVMIFLQKYGISLN
                     LGAKIYQKYKESVYTILQENPYRLAEDISGVGFKIADEIAARVGIHADSDYRIRSGML
                     YTLLQASGEGHTYLPREQLFTRCARLLGVDESYMEKHLMDMVIDRKLVLKEKSGETIV
                     YPAQYYYLELNTARMLNELNIVCPEDKELVRHRIELIEKETGTVLDEMQKKAITEAAD
                     HGLFILTGGPGTGKTTTINAIIRFFEGEGAEIRLAAPTGRAAKRMTETTGYEAQTIHR
                     LLELNGMPEEERDGHSAKFERNAQNPLEADVIIIDEMSMVDIHLMHSLLLAVVAGTRL
                     ILVGDENQLPSVGPGNVLRDIIRSRCFHVVELTKIFRQASESDIVVNAHKINKGEQVQ
                     INNKSRDFFFLKRYDADIIIRVVIALIQEKLPRYVDAKPFEIQVLTPMRKGLLGVERL
                     NQILQRYLNPPEDGKSERAVGDRLFRTGDKVMQIRNNYQMEWEIRGRYGVVIEKGVGV
                     FNGDTGILREINEFAETAEVEFEDGRFAMYSFKQLEELELAYAITIHKSQGSEYPAVI
                     LPLLSGPQMLLNRNLLYTAVTRARKCVTVVGKEETFAEMIRNEKQQKRYSALDERIRE
                     LSETTGDNNTDGEE"
     gene            10435..11139
                     /locus_tag="ACZPD3_00815"
     CDS             10435..11139
                     /locus_tag="ACZPD3_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR1414595.1"
                     /translation="MKRFLNMVADIFYPRCCPVCQKILADQRRMICPECEKELRPIGH
                     PRCYKCGKPIETGEYCRDCQKHRHMYEQGRGIFVYDGIMRRSVTRYKYYGCREYGDFY
                     ARAMYRYAQKELREWKPDLIVPVPVHRSKERQRGFNQAAYLAEKLGHYTGISTDVNIV
                     QKVLKTKSQKKLNALQRRKNLEKAFCVTGDVRGKNILVIDDVYTTGSTIDAMAGCLKR
                     KGAGNVYFLTVCIGRR"
     gene            11283..11717
                     /locus_tag="ACZPD3_00820"
     CDS             11283..11717
                     /locus_tag="ACZPD3_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414596.1"
                     /translation="MLNEEKIKIMNKLAMYEQGEGKKYLPVSRYYRSDYIGLAMIKNF
                     FLVTIGYCLILAGIAAYFAEYLIDNVHKMNLVSLGVEVILGYVAVLVLFSVLTYIQYT
                     VKYHKAKKSVKNYYEELTQLSKIYGREDKKSSARGVTGGYKK"
     gene            11714..>12523
                     /locus_tag="ACZPD3_00825"
     CDS             11714..>12523
                     /locus_tag="ACZPD3_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414597.1"
                     /translation="MTALLELKQRIKNLYSQYEIYILPVLRFVLAMVYFIWINTNMGY
                     MKQIDNIFIVLILALICSILPSGVMIFVGFALMVAHGYALGIEVAGFMLVLILFMAIL
                     FLRFSSDNNLVLVFTPLSFGFSVPTLLPIGSGLLCNAFSALPAGCGVIIYYFIRFIRV
                     QHKLLENPDVAIADKLKLLTDGIVQNWGMWITVIAFIAVILLVNLIRTRSFDYAWRIA
                     IIAGGVVYVLMIIAGGFYFRLDIDVVTLRILTKRAACVSAGDCSRNRINTCD"
BASE COUNT         3837 a         2305 c         3251 g         3130 t
ORIGIN      
        1 aaaagcctca gcgtagcccg ctacgcctgc gtttttatac cagcacactc gaatccgcat
       61 cctacgcgat tggtatagtt atttacgaaa cgatgtacta ggatgtttct ggttactgtt
      121 catatctact atcccgcgag gtgtgtgaac agtaacaaca gtaactgttt ctgtgcgtcc
      181 aatgtagata aatagatgac aaacccttcc cccatatgtt ataatacaaa attagcaaac
      241 ccatcaatta cagtatataa gaaagaactc aggagggaat tccttaatga tagaccagag
      301 caaaatcaga aatttctgta ttattgcaca catagaccac ggcaaatcca ctcttgccga
      361 ccgaatcatc gaaatgaccg gcactctgac agaacgtgaa atgcagtcac aggtactgga
      421 taacatggaa ctcgaaagag aacgtggaat caccattaaa tctcaggcag tgcgaatcgt
      481 atacaaagcc aaagacggcg aagaatacat cttcaacctg atcgacaccc ccggccatgt
      541 agacttcaat tacgaagtat cccgaagcct tgcagcctgc gacggtgcaa tcctggtagt
      601 agatgcagct cagggaatcg aagcccagac actggcaaat gtatatcttg cccttgacca
      661 tgacctggat gtccttccgg tcatcaacaa aatcgacctg ccaagtgcag aaccggatcg
      721 tgtggtaaat gaaatcgaag atgtcatcgg ccttgaagcc catgacgcac caaggatttc
      781 tgccaaaaca ggactcaatg tagaagaagt cctggaacag atcgtaacaa agatcccggc
      841 acctcatggc gatgtagatg caccattaaa agccctgatc tttgactcca tttatgatgc
      901 atacaaaggc gttatcgtat tctgccgtgt catggatggc cgtgtgaaaa gaggcaccca
      961 gatccatatg atggcaactg gcttcaccac agaagttgtg gaagtcggat actttggtgc
     1021 aggtcagttt attccatgtg aggaactgac agcaggtatg gtaggataca tcactgcgag
     1081 tattaaaaac cttggtgaca ctcgtgtagg tgatacagtt acagacaaaa acagaccatg
     1141 tgcagaagca cttccgggtt ataagaaagt aaatcctatg gtatactgcg gattatatcc
     1201 ggcagacggt gccaaatatg gtgatctgag agatgccctt gagaagcttc agctcaacga
     1261 tgcctctctg ttctatgagc cggaaacatc agtagccctt ggcttcggct tccgatgtgg
     1321 attcctggga ttacttcatc tggagattat tcaggaacgt ctggaaagag aatacaacct
     1381 tgatcttgtg accacagctc ccggtgttat ttataaagta tacaaaacta acggcgaagt
     1441 tatcaatctg acaaacccaa gcaatctgcc ggatccatca gagattgaat acatggaaga
     1501 accaatggta aatgccgaga tcatggtaac aactgaattt atcggtgcaa ttatggatct
     1561 ttgccaggaa cgaagagggc agtatctggg aatggattat atggaagaaa cacgtgcgct
     1621 tctgaaatat aagctgccgt taaatgagat tatctatgat ttctttgatg ccctcaaatc
     1681 ccgctccaga ggctatgctt ctctggatta cgaactctgc ggatatgagc gcagcgagct
     1741 tgtgaaactg gatattctgg taaacaaaga agaagtggat gctctttcct ttatcgtgca
     1801 tgcagataca gcatatgaga gaggccgtaa gatgtgcgag aaattaaaag acgagattcc
     1861 ccgccagctt tttgagattc cgatccaggc agctgtcgga agcaagatta ttgccagaga
     1921 gacagtcaga gccatgcgta aggatgtact tgccaaatgt tacggcggtg atatttcccg
     1981 taagaagaaa cttctggaga aacagaagga aggtaagaaa cgtatgcgtc aggtaggcaa
     2041 tgtggagatt ccgcagaaag catttatgag tgtattgaaa ctggatgata aataaagtat
     2101 gaatggtaac tattcggcag cctgctgtcc acataactgt gaaggtactg aacagttatc
     2161 tgtgaatagt gctcatctca acagaaggtt aaaatattga agaaaatgat taagcgaaaa
     2221 acagttatac tatgtatgtt attgcttgga atttctgtct ccggatgtgg gaaaaccagg
     2281 aaggtagatg aagcgtcctc caaagtgatc cagatcagtg ttgcgccgga agagacaagt
     2341 cccactccgg caccggatca ggtagcttcc gcagcagtta ccaccaatgg taatctgaca
     2401 atggtaaata cgtatctggc agagcaggat gcagcaggta tctcatctgc ggaaagttca
     2461 gacagccaaa gtgatgccga cagtacagat aacagtgtcg actcagcaga taacaatgag
     2521 caagacagca gtacatcaga tactgaggaa tagaaaacag gtcataacag gagcagatta
     2581 caatggagag aaggaatatc acagattctc ccatggaaat ctatgtccac attccatttt
     2641 gcataaaaaa atgtgattac tgtgatttcc tttccggtcc gtcaggtcca aaggagcagg
     2701 ctgattatgt agatgctctg cttgaagaaa tcaatgcagc agaagaagga aaaggaagga
     2761 gtgtatcctc tgtatttatt ggagggggca ctccttctgt tttggatgag agattcatag
     2821 gagaaatttt aaatcatata agacgaaaat ttcagattgc ggatcatgct gagatcacca
     2881 tagaagtaaa cccggggact gcagacagaa acaaactgca ggcatacaga acatatggaa
     2941 tcaaccgcct gagcataggt ttacagtcgc cggatgacag agaactgaag atccttggaa
     3001 gaatccacaa ttatgagcag tttctggaga cttacagaag tgcaagagag gcagggttcg
     3061 acaatatcaa tattgatctg atgagtgcga tcccggatca gacttatgaa ggatggatcc
     3121 acaatcttcg cactgttgca ggactggatc cggaacatat ttccgcatac agcctgatca
     3181 ttgaggaggg cacacctttt gcatcgcgga cgctgaattt gccggatgaa gatgcggagt
     3241 acaatatgta tgaggcaaca gcacagattc tgagagaata tggctttgag cagtatgaga
     3301 tttccaatta cgcaaagaaa ggcagagaat gcaggcataa cgtgggctat tggatccgac
     3361 aggattatct gggatttggc ctgggagctt cctctcttta tggaaaggaa cgttttgcca
     3421 atacacagga tatgaaaaaa tatctggaaa acagcagaac tccggaaaaa ataagagaaa
     3481 aagagccgcc cctcaccaga gaggatgaga tggcggagtt catgtttctg ggacttcgta
     3541 tgaccagagg aatctcaaaa gctgagtttg agcggcagtt tggaagtgaa atagatgcaa
     3601 tatacgggga tgttctgaga aaatataagt caatgggact gcttttggaa gaaaacggaa
     3661 ggatttttct gagcagagag ggaattcatg taagcaattc cgttatggcg gattttttgc
     3721 cgtaaacgcc ttgacgaaca aatgttcctg tattatactg atgccatgag gattgcgggg
     3781 cagcgaaact gcgcacaatc catgaatatc ataacatacg aaaaaggtgt aactgtcaag
     3841 gtaagaaaca gttattattt ataaatgaga aacaggaggc ccacaatggc agcagataca
     3901 acgaagaagc actttatcag gatcagaggt gccaatgtta ataaccttaa gaatttaagt
     3961 gtggatattc ccagagacaa gtttgtggta tttacaggac tgagtggctc cggtaaatct
     4021 tcactggctt ttgataccat ctatgcagag ggtcagcgtc gttatatgga atctctctct
     4081 tcctatgcaa gacagtttct gggtcagatg gagaagccgg atgtggagag catcgaaggt
     4141 ctgccaccgg ccatttccat tgatcagaaa tctaccaacc gcaatccccg ttccacagta
     4201 ggaacagtga cagaaatcta tgattatttc cgacttctgt atgccagagt gggaattcct
     4261 cattgtccga agtgcggaag agagattaag aagcagacag ttgaccagat ggtagacagt
     4321 atcatgtctt ttccggagag aacgaagatc cagcttctgg cgcctgttgt gcgggggcgc
     4381 aagggaactc atgcaaagct tctggagcag gcaaagagaa gcggatatgt acgtgtgcag
     4441 attgacggaa acctgtatga gctttccgag gagatcagcc tggacaagaa tatcaagcat
     4501 aatatagaga ttgtggtaga ccgtctgatc gtgaagcccg ggattgagaa aagactctca
     4561 gattccattg agacagtgct gaatctggca gagggccttc tgatggtaga tactatggat
     4621 ggaaatattc acaatttcag ccagagtttc tcctgtcctg actgtggtat cagtgtagat
     4681 gagatcgaac cgagaagctt ttctttcaat aatccttttg gcgcctgtcc ggactgtctg
     4741 ggacttggat ataaaatgga atttgatatt gatctgatga tcccggatag atctctgagt
     4801 atcctggatg gtgccattgt ggtcacagga tggcagtcct gcacaaatga gggaagcttt
     4861 tccagggcaa tcctggatgc tcttgccaga gaatatgatt tcagcctggc aacacctttt
     4921 gaggagtatc cggagaagat acaggacatt ctgatcaacg gaactaatgg acattctgta
     4981 aaggtttact ataaagggca gcgcggagag ggcgtttacg atgtggcgtt tccggggctg
     5041 atccgaaatg tggagcagcg ttacagagag acaggctctg atgccatgaa gcaggagtat
     5101 gaatctttca tgcgcattac tccatgtaaa acctgtaaag gtcagagact gaagaaggaa
     5161 tcactggctg tgactgtggc ggataagaat atttatgagg tcaccaatct ttccatagag
     5221 aagctgaaag cctttctggc ggatatgcag ctctccgagc agcagcagct tatcggcaga
     5281 cagattctga aggaaatcag ggcaagggta agtttcctgt cagatgtagg actggattat
     5341 ctttcattgg gacgtgcgac aggaactctt tccggcggcg aggcgcagag aatccgtctg
     5401 gctacccaga ttggttccgg gctggtagga gttgcctata ttctggatga accgagtatc
     5461 ggacttcacc agagggataa tgacagactt cttggttctc tgatgaaact gcgtgatctt
     5521 ggaaacagcc tgatcgttgt ggagcatgat gaagatacca tgcgtgccgc agactgtatt
     5581 gtagatatag gtcccggtgc aggggagcat ggcggacaac tggttgccat gggaactgca
     5641 gaggatttga tgaagaacga ggattccatc acaggagctt atctgagcgg aaaattaaaa
     5701 attccggtac ctttggagag acggaagcca accggttttc ttactgtcaa gggggcggca
     5761 gagaataatc tcaagaatat cgatgtgaag atacctcttg ggatcatgac ctgtatcaca
     5821 ggagtttcgg gctcaggtaa gagctctctg atcaatgaga ttctttataa acgcctggca
     5881 agagatctga accgggcaag agtgattccg ggaaaacata aggacattct gggaacagac
     5941 caactggata aggttatcaa tattgaccag tcacctattg gccgtactcc acgttccaat
     6001 cctgcgactt ataccggggt atttgaccag atcagagatc tttttgcagc aacagcagat
     6061 gccaaggcaa aaggatataa gaaaggacga ttcagtttta atgtaaaggg cggaagatgt
     6121 gaggcatgca gcggtgacgg tattatcaaa attgagatgc atttccttcc ggacgtatat
     6181 gtaccatgcg aggtctgcaa aggtaagaga tataacagag aaaccctaga agtaaagtat
     6241 aaggataaga atatttacga tgtgctgaat atgacggtgg aggaagcact gacttttttt
     6301 gagaatgttc cttctattaa aagaaagata cagactcttt atgatgtagg actttcctat
     6361 atccggttgg gacagccttc cacagagctt tccggaggag aggcacagag gatcaagctt
     6421 gccacagagc tgagcaagcg cagcacaggt aagactatct acattctgga tgagcctacc
     6481 acaggtctgc attttgcgga tgtgcataag ctggtggaga ttctgaaacg attatcggag
     6541 ggcggtaata cagtagttgt gatcgagcat aatctggatg tgattaagac cgcagattat
     6601 atcattgata tcggaccgga gggcggagac aaaggaggta ccgtagttgc tcagggaact
     6661 cctgaggagg tggcacagtc accggtttcc tataccggaa aatatgtaaa gaaatatctg
     6721 aaataatctt gaaatcatat tgcaggactt tctttttaaa tggaagttgt atataataga
     6781 agagattatt tatagcaatt ctcgtaaata atatattttg atccggctta tcaggatgtg
     6841 aagccctgtt ggactgtttt gatcgcatta ttttatggag ttgttataac taaaccggat
     6901 aagtccccgg tagagactgt aaaaagtaat gatcagttga aggggatgag gagaacgtaa
     6961 gaacagaaag gggatttcca tgccgacaag tgatattgga attgacctgg gaaccaggaa
     7021 cagtctggct tattctacag gaaaggggct tgtgctaaat gagccttcga tcgtagttta
     7081 tgataaaaat acagagaaga tacgtgcaat cggtgaagaa gcccgtctga tggaagggcg
     7141 cattacttca gatatggaga tcatccgtcc catcagacag ggcgtgatcg ttgattatac
     7201 agttacagag aagatgttga aatactttat atccagagca ataggaagaa gagctttccg
     7261 caagcccagg atcagtatct gtgttcccag tggaattaca gagattgaga aaaaggcagt
     7321 ggaggaagca acctatcagg ctggtgccag agatgtctat atggtggagg aacctatagc
     7381 tgcggcaatc ggagccgggg tggatgtgac caagcccttt ggaaatctgg tcgtagacat
     7441 tggagccgga acttcggatg ttgcggttat ctctcttgcg ggggttgtgg tttccgcttc
     7501 tgtgaaagtt gcaggggaca cttttaatca ggctatactg aactatgtga gaaagaatca
     7561 tggacttttt atcggagaag atatggcaga gaagatcaaa attcagattg gtacggctat
     7621 agaggaatca aatccgagaa caatggaagt aaaggggaga aatgtgatca ccggtctgcc
     7681 gaagacagtg acacttacct cagaagaaat acgtgtggcg ttaaaggatg ctacaagcca
     7741 gattgtggag actgttcatg gaattctgga aaagacaccg ccggaactgg ctgcggatat
     7801 tgtagacaga ggaatcgtac ttacaggcgg cggtgccctt cttcatggga tggatacact
     7861 gatcgagcag agaaccgggg taagtacact gactgtgcag gatgcaatga gcgtggtcgc
     7921 agtgggaacc ggtaagtatg cagaggtaat ggcacgtttt gacggataaa gcaggaggta
     7981 ctgcacagtg cagtgccttt ttgcgttgtc agaggctcaa agaagcagga ggtgtggaga
     8041 aaagcacatg agtgaaactg tcacaggata catagatcat gttattttca gaaatgaaga
     8101 taacggatat acagttatgg tactgaaagg tacaaaaaaa gaggaagagc tgacctgcgt
     8161 gggaagtttc cctgcgatca cacagggggc atctattgaa gcaaccggag tatatatcca
     8221 tcatccggta tatggcaaac agtttcagat cagttctttt acagagaaga tgccggagga
     8281 tacatatgga atagaacgtt atctgggttc gggcgccatc aggggaatcg gtgctgcact
     8341 ggctgccagg atcgtgcgga agtttggaga tgacacactc cgtatcgtag aagaagagcc
     8401 ggagcggctt gcagaggtaa aggggatcag tgagaaaaaa gcccgggaga tcgctgcgca
     8461 ggtaagtgag aaggcagaga tgcgaaaagt aatgatcttt ctgcagaaat atgggatttc
     8521 cctgaacctg ggcgcaaaga tttatcagaa atataaggaa tctgtctata cgatccttca
     8581 ggagaatcct tacagacttg cggaagatat ttccggtgtg ggatttaaga ttgctgatga
     8641 gattgctgcc agagtcggta ttcacgcaga ctcagattac agaatccgca gcggaatgct
     8701 gtatactctt ctgcaggctt cgggagaagg acatacttat cttccgaggg aacagttatt
     8761 tacaagatgt gccagacttc tgggagtaga tgaatcctat atggagaaac atcttatgga
     8821 tatggttatt gacaggaagc tggtgctgaa agaaaaaagc ggagaaacta tcgtttatcc
     8881 ggcacagtat tattatctgg aactgaacac tgccaggatg cttaatgaac tgaatattgt
     8941 gtgtcctgag gataaagagc ttgtcaggca cagaattgag ctgatcgaga aagagacagg
     9001 aactgttctg gatgaaatgc agaagaaagc aatcactgaa gcagcggatc atggactctt
     9061 tattcttaca ggaggccccg gtacaggtaa aacgacaacg atcaatgcga tcatccgctt
     9121 ttttgaggga gaaggtgcag agattcggct ggctgcacct accggacgtg ctgcaaagag
     9181 aatgacagag actaccggat atgaggcgca gacaattcac cgtctgctgg aactgaatgg
     9241 aatgccggaa gaagagaggg acgggcattc agctaaattc gagaggaatg cgcagaatcc
     9301 gctggaggca gatgtgatca ttattgatga aatgtctatg gtggatatcc atctgatgca
     9361 ttctcttctt cttgcagtgg tagcaggaac cagactgatc cttgtggggg atgagaatca
     9421 gcttccgagt gtaggcccgg gcaatgtgct gagagatatt atccgaagca gatgtttcca
     9481 tgtagtggaa ctgactaaga tattcagaca ggcatcagag agtgatattg ttgtaaatgc
     9541 tcataagata aataagggag aacaggttca gatcaataat aagagcagag atttcttttt
     9601 tctgaagaga tatgatgcag atatcattat aagagtagtg atcgcgctga tccaggagaa
     9661 actgccgcgc tatgtagatg ccaaaccctt tgagatacag gtactgacac ctatgagaaa
     9721 gggacttctg ggagtagagc ggctgaacca gatcctgcag agatacctga atccgccgga
     9781 agacgggaaa tccgagaggg cggttggaga ccggctgttc cgcacagggg acaaggtcat
     9841 gcagatcaga aataattatc agatggaatg ggaaatccgg ggacgatatg gggttgtcat
     9901 tgagaaaggc gtgggagtat ttaacggaga caccggaatc ctcagggaaa tcaatgaatt
     9961 tgctgagaca gcagaagtgg aatttgagga tggacgtttt gcaatgtact cgttcaaaca
    10021 gctggaggaa ctggaacttg catatgctat tacaatccac aagtctcagg gatctgagta
    10081 tccggcagtc atacttccat tgctttccgg accacagatg cttttaaaca ggaaccttct
    10141 ttataccgca gttaccagag caagaaaatg tgtgacggtt gtgggaaaag aggagacctt
    10201 tgcagagatg atacgcaatg aaaaacagca gaagagatac agtgcactgg atgaacggat
    10261 cagagaactg agtgagacta caggggataa taatacagat ggtgaagaat gatattgcag
    10321 actgtgatct gatgactgaa taataacaga gaaaaaattt attccgataa tatgaaaaaa
    10381 acagacatac agaggatagc aaaacaaaga gtaaaatagt ggaaggaaat ggaaatgaaa
    10441 cggtttctga acatggttgc tgatattttt tacccgagat gctgtccggt ctgtcagaaa
    10501 atactggcag atcagcgaag gatgatctgt ccggaatgcg agaaagaact tcgtccgatt
    10561 ggtcatccca gatgttataa atgtggaaaa cccatagaaa caggggaata ctgcagagac
    10621 tgtcagaaac acaggcatat gtatgaacag ggaagaggta tttttgttta tgatggcata
    10681 atgagaagat ctgttaccag atataagtac tatggatgca gagaatacgg tgatttttac
    10741 gccagagcaa tgtatcgata tgcacagaaa gaattgcggg aatggaagcc ggatctgatc
    10801 gtgccggttc cggtacacag gtcgaaggag cggcagagag gttttaatca ggctgcatat
    10861 ctggcggaga aactcggtca ttataccggg atttccacag atgtcaatat tgtgcagaaa
    10921 gttttaaaga caaagtcgca gaaaaaactg aatgctttgc agagaagaaa aaacctggaa
    10981 aaagcgttct gcgtgaccgg agatgtcagg ggaaagaata tcctggtgat agatgatgtg
    11041 tatacaacag gaagtacgat cgatgcaatg gccggatgcc tgaagagaaa aggtgccgga
    11101 aatgtttatt ttcttacggt ttgcataggc aggagataag aaaaaacact tttcatacat
    11161 aatgatatat gttacaatta atctgtatga ttatatgaat gatatcaggt tgttgctgag
    11221 aacagagtga acagtaatag cggattatag caaaaggaaa agcatacagt aaggagtaat
    11281 ttatgttaaa tgaagagaag ataaaaatca tgaataaact tgccatgtat gaacaggggg
    11341 aaggaaagaa atatcttccg gtcagcaggt actaccgtag tgattatatc ggacttgcca
    11401 tgattaagaa ttttttcctt gttacgattg ggtattgcct gattctggcc ggtatcgcag
    11461 catattttgc tgaatatctg atagataatg ttcataagat gaaccttgtt tcactgggcg
    11521 tggaagttat tctggggtat gtggcagtgc tggtactgtt ttctgtcctg acatatatcc
    11581 aatatacggt gaaataccac aaagcaaaga aaagtgtgaa aaattattat gaagaactta
    11641 cacaattaag taaaatctac ggtcgcgaag ataaaaaatc ttcagccaga ggagttacag
    11701 gaggatataa aaaatgacag cattactcga attaaaacag cgaataaaga atttatacag
    11761 ccagtatgag atttatatcc tgcctgtctt acgtttcgtt ctggcaatgg tttatttcat
    11821 atggatcaat accaatatgg gatatatgaa gcagatcgat aatatattta ttgttctgat
    11881 ccttgcactg atctgttcca ttcttccttc aggtgtaatg atttttgtag gatttgccct
    11941 gatggtcgca catggttatg cactggggat agaagttgca ggttttatgc ttgtgctgat
    12001 cctgtttatg gcaattctct tcctgagatt cagttcagac aataatttag tacttgtatt
    12061 tacaccgctt tcctttgggt tcagtgttcc gacgcttctt cctatcggaa gcggactttt
    12121 gtgcaatgcg ttttctgcac ttcctgcagg ctgcggagtg attatttact attttatccg
    12181 ttttatacgt gtacagcata aacttctgga aaacccggat gttgccatag ctgacaaatt
    12241 aaaacttctg acagatggaa tcgtgcagaa ctggggaatg tggatcacag tgattgcctt
    12301 tatcgcagtg attctgctgg tgaatctgat acgtacccgc tcttttgact atgcatggag
    12361 aattgccatt attgcaggtg gagttgtgta tgttctgatg attattgcag gtggatttta
    12421 ttttagactg gatattgatg ttgttacgct gagaatactg accaagcgag ccgcctgcgt
    12481 ctctgccggt gattgttccc gtaacaggat caacacttgc gat
//
LOCUS       DBMDXG010000011        12024 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1516_length_12024_cov_2.362577, whole genome shotgun sequence.
ACCESSION   DBMDXG010000011 DBMDXG010000000
VERSION     DBMDXG010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12024)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_12024_cov_2.362577"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="ACZPD3_00830"
     CDS             <1..926
                     /locus_tag="ACZPD3_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR1414598.1"
                     /translation="GSRMLVGKNGRISGAIGGGAVEYRAECMALDILEKKESCEHEFR
                     LNHKDVENIGMICGGDVTAFFQYLDHNDPIIMEITETAEKFYEERKDFWLICDLLTTS
                     GVSLYSPACGLIGTANVPSSVLSSLSLKPHRYHSEDYDLFSEQIGTSGTVYVFGGGHV
                     SQKLVPILASVDFRCVVLDDRPEFTNPALFPGAVETILCDFNHLDQYVSITAADYCCV
                     MTRGHSYDTIVQAQLLATPACYIGVIGSRAKKAAVFRRLIEEYNINEQDLNRIISPIG
                     LEIKAETPAEIAISITGQMIQVRADRKDTSK"
     gene            923..1783
                     /locus_tag="ACZPD3_00835"
     CDS             923..1783
                     /locus_tag="ACZPD3_00835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HIR1414599.1"
                     /translation="MMAKSVYKTVIFGAGQIGQMTARLLSSPCQLLCFADNDPHKHGS
                     YIGNIPVCSPDTAAALLPDLVILGVLDEERRNSMIKQMEYLGYHGPFRDPSVLRMFDA
                     RVAVMRLLSEQIYQLDIPGNVAELGVFRGEFSSLISAAFPDRKIHLFDTFEGFSEKDI
                     TIEASGNLSRAKTGDFSSTDIDSVLHVMPNPTRTVIHKGWFPDTFSDVTDETFCFVSL
                     DADLYAPTAAALPLFYERLATGGVLLIHDVYSTQFSGCRKAVGEFCLKNHLFADPVCD
                     LHGSAMIRKL"
     gene            complement(2122..2195)
                     /locus_tag="ACZPD3_00840"
     tRNA            complement(2122..2195)
                     /locus_tag="ACZPD3_00840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2159..2161),aa:Arg,seq:acg)
     gene            complement(2387..3619)
                     /locus_tag="ACZPD3_00845"
     CDS             complement(2387..3619)
                     /locus_tag="ACZPD3_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1414600.1"
                     /translation="MSNLYEYIKMAVHNIMANKGRSFLTMLGIIIGIASVIAIVSVGE
                     GTKNQMNSEIDDIGGGQIAISVSDEAQTDEEWITADDVEAIRAVDGIEGVNVSDSFSG
                     ETTTGKGNFSLMLTGEGPDAKLLNNATMKHGVYFGENEVQEAKNVCVLSDADAKRLFG
                     TDDVVGMTVDVNCYGLTKSLRICGVTTQKENGTFVSYTYEGMPVTINVPYTTMNEFTG
                     VSDYFFSVTMQADKSLNSQDVADKVVKLMEKRHQCAGKDYFQVQSFQDIMSSMNQMLD
                     MVTAFISFVAGISLLVGGIGVMNIMLVSVTERTREIGIRKSLGAKTSSIMMQFLAEAA
                     ILTVIGGVIGIVLGVIGGYVICSIISSSMGMSITPGISLSTIMAATLFSCAVGVFFGI
                     YPAKKAAKLSPIEALRRN"
     gene            complement(3612..5444)
                     /locus_tag="ACZPD3_00850"
     CDS             complement(3612..5444)
                     /locus_tag="ACZPD3_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR1414601.1"
                     /translation="MKKKKSKKKIIIGAAAVLVVAGGVTVAGQRNSTENQIPQVQVVI
                     AEKGDVEEIVDATGTVGSEEEKTYYSPVNAELKTVSFAQGDVVKKGTKLIEFNTEDLE
                     KDNRKAELNLKSTKYDTKDTRNKSYKAEKKQKDAKKNVQELEKKIKDKKAYVSSLKSQ
                     ISAAAAAAQREAAAQASAQAAAQAQAQQQEAQAKAQAEAKKQEEIQSKYQAALYTYKT
                     ETLPQYQQQLSDLNAQYNQAQSDYNQADTTYQMAFATWQADPSDENTQALDNAEAART
                     QAQLAMQQAQQTYNDLKQQTPKMPVLSDFTESSADYSWGISDGSDADDSDGEDSSSYG
                     YDYSGSDGGTVTADTSALESALETASDELAELQSDLASEKAIAEADSTSLTKEEKEKL
                     KVTDNLSELDAKSAEELVKEGKKGITAEFNGIISKADIKQGAAVTQGMELFTIENADK
                     ASVDVTLSKYDYNTVKEGQSVEITLGDNTYQGTVTKMSHIAVQNEKGTPVISATVSID
                     NPDEDIFLGVDAKVKIYAASAKNVVTLPVEVVNIGKEGSFCYVIEDGLVTKRNITTGI
                     SSEDYVEITDGIKEGEEVIADLGDYTEGMEVQAVPEQTGEDADE"
     gene            complement(5470..6201)
                     /locus_tag="ACZPD3_00855"
     CDS             complement(5470..6201)
                     /locus_tag="ACZPD3_00855"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414602.1"
                     /translation="MEEALIRVKDLCKIYNPGENEVRALDHINLEINKGEFVAIIGQS
                     GSGKSTFMNMLGCLDVPTSGEYFLNGTDVSTMEDNELSEVRNREIGFIFQGFNLISNL
                     TAIENVELPLVYRGVDRKTRHKLAVESLTMVGLEKRMDHRPNEMSGGQQQRVAIARAI
                     AAQPPVILADEPTGNLDSASSKEILQILKSMHEQGKTVILITHDNGIAAQARRVVRIM
                     DGKIESDFINKNYGKEEYIKNQLDA"
     gene            complement(6442..7833)
                     /locus_tag="ACZPD3_00860"
     CDS             complement(6442..7833)
                     /locus_tag="ACZPD3_00860"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1414603.1"
                     /translation="MERRNAWLSYTEAEEKELEQVAKAYRNFLNVGKTERECVTQIIR
                     EARAAGYESLEEKTAKGEKLKAGDKVYTVGMKKIIALFHIGQDDISEGMNILGAHIDS
                     PRLDVKQNPLYEDTDLAYLDTHYYGGVKKYQWVTLPLAIHGVVVKKDGSTAEVNIGED
                     EDDPIVYITDLLIHLAGKQMQKKAAEVIEGENLDILIGSRPLKDLEDDKKKEAVKQNV
                     LNILKEKYDMEEEDFLSAELEIVPAGKARECGLDRSMIAAYGQDDRVCAYTSLLAMLG
                     MDTPKHTSCCLFTDKEEIGSVGATGMQSRFFENAVAELLDAMGCYSDLRLRRTLKNSS
                     MLSSDVSAGYDPAYGEAFEKKNAAYLGRGIVLNKFTGARGKSGSNDANAEYVARVRNI
                     FDSHEVAFQTAELGKVDVGGGGTIAYIAALYGMEVIDSGVAVLSMHAPWEVTSKADIY
                     EAKKAYKAFLLEA"
     gene            8109..9851
                     /locus_tag="ACZPD3_00865"
     CDS             8109..9851
                     /locus_tag="ACZPD3_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HIR1414604.1"
                     /translation="MKNSEELRQQLRSINRKSYPAYKGLKGLYHFGNYILSIDHVQGD
                     PFASPSHVSIQISHRDTGFPVEYYKDTLTGTTLCDYLTRQFEKQVSQYSFRAKGSGKS
                     GLLTASHCGQEILSRTACEITEKGITARFFVGFPANGRTINATELEKILFDFLPVCIQ
                     KSFFYSSLNAKELQNYIELAEDQEFIRQTLPAKNLCAFIADGSILPRESGISSRPMKA
                     SIPFTSPDSLRISINLPHKGKITGMGIPKGITLIVGGGYHGKSTLLNALELGVYNHIP
                     GDGREYVITDATAVKLRSEDGRFIKDVDISMFINDLPNKKDTRCFSTLDASGSTSQAA
                     GIVESMEAGSHLFLLDEDTSATNFMVRDAFMQQVIQREKEPITPFLERAEDLYKKAGI
                     STILVAGSSGAFFHIADTIIQMDNYVPKDITASVKKLCSQYPLPAVSVTDFQLPHSHR
                     IMSRPAESSKRLIHNSRGNHSDSGAAKPERLKTRISGTDGFSLGRQEIDLRYTEQLID
                     AEQTAALGLLLKYAVEHLADGRRTLPEIVQFLWKNLSLHGLSFFTENQKISCGYATPR
                     IQEIYACLNRYRGL"
     gene            10351..11088
                     /locus_tag="ACZPD3_00870"
     CDS             10351..11088
                     /locus_tag="ACZPD3_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR1414605.1"
                     /translation="MFRKIDKTSTFNDVLKQIIENIQNGELQPGDALPAERILAEEFG
                     ISRPALREVLKALSLLGITVSVHGGANYIATDLHSCLTEPLSIIFQMYNSKIQDALQL
                     RGALESKAAFLAAQNCTPLDAAELQLIIARLDSTQDEKIRGDLDRDLHLKIAQISDNP
                     LILSVLNASAQITENMIVGIRSYLMQKNNDAPEIDSQHTRLVQAITNNDAPLAEKVMN
                     EHMHTIEYLLNEITSDNLTISDNVQQP"
     gene            complement(11198..11488)
                     /locus_tag="ACZPD3_00875"
     CDS             complement(11198..11488)
                     /locus_tag="ACZPD3_00875"
                     /inference="COORDINATES: protein motif:HMM:NF044580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HIR1414606.1"
                     /translation="MENVIRKLYGMYMDNDNREKNEMSVMHDDLCKEKKEKAYQCYQK
                     LRVQLTDELAEELDELMNKHMEIYPQELEESFAMGFKTGARLMCEIFSEETE"
     gene            complement(11520..11624)
                     /locus_tag="ACZPD3_00880"
                     /pseudo
     CDS             complement(11520..11624)
                     /locus_tag="ACZPD3_00880"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            11583..11855
                     /locus_tag="ACZPD3_00885"
                     /pseudo
     CDS             11583..11855
                     /locus_tag="ACZPD3_00885"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
BASE COUNT         3159 a         2945 c         2171 g         3749 t
ORIGIN      
        1 ccggttcccg catgctggtc ggaaaaaacg gcagaatttc cggagccatc ggaggtggag
       61 ctgtagaata tcgtgctgaa tgtatggcat tggatattct tgaaaaaaag gaatcctgcg
      121 agcatgaatt ccggctgaat cataaagatg ttgaaaatat tggtatgatc tgtggtggtg
      181 atgttacggc ctttttccag tatcttgatc ataacgatcc gattattatg gaaatcacgg
      241 aaactgctga aaaattctat gaagagcgaa aagatttctg gctgatttgc gatcttctca
      301 ccacctccgg tgtgagcctt tacagtcctg catgcggtct catcggaact gccaatgttc
      361 cttcttctgt attatcttct ttgtctctaa agccacaccg atatcacagt gaagattatg
      421 atctgttttc tgaacagatt ggaacctcag gaactgtcta tgtattcggc ggcggacatg
      481 tctcacaaaa gcttgtccca atccttgcaa gcgttgattt tcgctgtgtt gtcctggatg
      541 accggccgga atttacaaat cctgctcttt tcccaggagc agttgaaaca atactttgtg
      601 atttcaacca tctggaccag tatgtttcta ttacagctgc tgattattgc tgcgtcatga
      661 cacgtggaca ttcctatgat accattgtac aggcacagct tctcgctaca cctgcatgtt
      721 atataggcgt cattggaagc cgtgcaaaaa aagctgctgt atttcgccgt ctgattgaag
      781 aatacaacat taatgaacaa gatttaaacc gtatcatctc acctatagga ctggagatca
      841 aagcagaaac accggctgaa atcgccatct ccatcacagg acagatgatc caggttcgcg
      901 ctgacagaaa ggatacatca aaatgatggc aaaatcagta tataaaactg taatatttgg
      961 tgccggacaa attggacaaa tgaccgcaag acttctcagc agtccctgtc agctcctgtg
     1021 ctttgctgat aatgaccccc ataaacacgg atcatatatt ggaaatattc cagtctgttc
     1081 tccggatact gcagcagcac ttctcccgga tctggtcatc ctgggagttc tggatgaaga
     1141 acggcgcaat tctatgataa agcagatgga atacttaggt tatcacggac cttttcgtga
     1201 tccctccgtc cttcgcatgt ttgacgcacg ggttgctgtt atgcgtcttt tatctgagca
     1261 gatctatcaa ttggatattc cgggaaatgt tgcagaacta ggtgtttttc gaggtgaatt
     1321 ttcttccctg atcagtgcag cctttccaga ccgaaaaatc catctttttg atacttttga
     1381 gggattttca gaaaaggata tcacaataga agcctccggt aatctgtccc gtgccaaaac
     1441 aggtgatttt tcttctactg atattgactc tgttcttcat gttatgccta atcctacacg
     1501 tactgtgatc cacaaaggat ggtttccgga taccttttcc gatgtcacag atgaaacttt
     1561 ttgttttgta tctctggacg cagatctcta tgctccaact gcagccgcac ttcctctgtt
     1621 ctatgaacgc ctggccaccg gaggagttct cctgatccac gatgtgtaca gtacacagtt
     1681 ttccggatgt cgaaaagcgg tcggggaatt ctgtctgaaa aaccatctgt ttgcagatcc
     1741 tgtatgcgat ctgcatggaa gcgcaatgat ccgaaaatta taaaaggaaa cgccggaacc
     1801 ggtatttacc ggaaccagcg tttctttttc atttatttat tgtatagccc ttttccacgt
     1861 ccatgcctaa cctttataaa attcctttca gaacattttc caacgcttct catggaagta
     1921 atttcaggac tggccaatca aaaaaataac cgctagaagc cttgatttct agcggttcta
     1981 cgtgcgttag aggttgctgc gtgccagtgg cacgcgttta gcaacgaccg gagcgaagcg
     2041 tagatcgaac ccgttcgagt ccgacccgta ctcgaataaa aaaataagac agtgcatttg
     2101 cactgtctta ttttttatta gcgtgcgtta gaggattcga acccccggcc ttttggtccg
     2161 tagccaaacg ctctatccag ctgagctaaa cgcacatcct tgtgaagttc ttatctcgtg
     2221 tccctcacaa caatgagtat tctactctat actggagcat ttgtcaacac tttttttgaa
     2281 ttttttattt ttttcttttg attcgcaaaa ttataacaat ttacaactat tctgtaatga
     2341 tatcagtaca caggtacacc aaaataccat cgcagtcctg ttttcattaa ttccgccgca
     2401 aagcctcgat cgggctcagt tttgctgctt tcttcgcagg ataaattcca aagaatactc
     2461 ctactgcaca ggaaaacaaa gtcgccgcca taattgtact caggctgatt cccggagtga
     2521 tggacattcc catactgctg cttatgattg aacagatcac atatccgcca atcacaccca
     2581 gaacaattcc tatcacccct ccaatcaccg tcagaattgc cgcctctgcc agaaactgca
     2641 tcataataga agaggttttc gctcccagtg acttacggat accaatctct ctggtacgtt
     2701 ctgttacgga aaccagcata atattcataa cgccgatacc gccaaccaga agggaaattc
     2761 ccgccacaaa ggagataaaa gcagtcacca tatccagcat ctgattcata gagctcataa
     2821 tatcctgaaa gctctgtacc tgaaagtaat cttttcctgc acactgatgt cttttttcca
     2881 tcagttttac cactttgtca gcaacatcct gactgtttaa gctcttatct gcctgcattg
     2941 ttactgagaa aaagtaatcg gatacaccgg tgaattcatt cattgtagta tagggaacat
     3001 taatagtcac cggcattccc tcataagtat aactgacaaa ggttccattt tctttctggg
     3061 ttgtcacccc gcagatacga agacttttgg tcagtccata acaattgaca tctacagtca
     3121 ttccaaccac atcatctgtg ccaaagagtc tttttgcatc tgcatcggaa agcacacaga
     3181 cattttttgc ttcctgaact tcattttctc cgaaatatac tccatgcttc atggtggcat
     3241 tatttaacag ctttgcatcc ggtccctcac ccgtcagcat aagagaaaaa tttcccttac
     3301 ctgtggtagt ttctccactg aatgaatccg aaacatttac tccttctata ccgtctaccg
     3361 cccggattgc ttccacatca tccgcagtga tccactcctc atctgtctgt gcctcatcgc
     3421 tgacactgat ggcaatctgc ccgccgccga tatcatcaat ttcactgttc atctggttct
     3481 tggttccttc acccacagat acaatggcaa ttacagaagc aatgccaatg ataatgccaa
     3541 gcatagtcag aaaagaacgt cccttatttg ccataatgtt atggactgcc atcttgatat
     3601 attcatacag attactcatc ggcatcctct cctgtctgtt caggaactgc ctgtacttcc
     3661 attccctctg tgtaatcacc cagatctgcg attacttctt ctccttcttt gattccgtcc
     3721 gtaatttcca cataatcctc tgagctgatt cctgtggtaa tatttctctt tgtcaccagt
     3781 ccatcttcta tcacatagca gaaggagcct tcttttccga tattgaccac ttccacagga
     3841 agagtcacca catttttcgc agaagccgca taaatcttaa cctttgcatc tactcccagg
     3901 aaaatatctt catccggatt atcaatgctt actgttgctg aaatgaccgg cgttcctttt
     3961 tcattctgca ccgcaatatg actcattttt gttacagtac cctgataagt attatccccc
     4021 aaggtaatct caacagactg cccctctttc actgtattat aatcatattt agagagagtc
     4081 acatccacac ttgccttgtc tgcattctca atagtaaaca gttccatacc ctgagtaact
     4141 gctgctccct gcttaatatc tgccttggat atgattccgt taaattccgc ggtgattcct
     4201 ttctttcctt ccttcaccag ctcctcggct gattttgcgt ccagttcgga aagattatct
     4261 gttactttca gtttctcttt ttcttctttt gtcaggcttg tggaatctgc ctctgcgata
     4321 gctttctcag aagccagatc tgactgaagc tctgcaagtt catcgcttgc tgtctcaagt
     4381 gcactttcca gagcagaagt atccgcagtt actgtcccgc catcacttcc actgtaatca
     4441 tatccgtagg aggaagaatc ttccccatcg ctgtcatctg catctgaacc gtctgaaatt
     4501 ccccatgagt aatctgcact gctttctgtg aaatcagaaa gtaccggcat tttcggtgtc
     4561 tgctgcttaa gatcattata agtctgctgt gcctgctgca ttgcgagctg tgcctgtgtt
     4621 cttgcagcct ccgcattatc cagagcctgt gtattctcat ctgaagggtc tgcctgccat
     4681 gttgcaaatg ccatctgata tgtggtgtct gcctgattat aatcgctttg tgcctgatta
     4741 tactgtgcat tcagatctga gagctgctgc tggtactgcg gaagagtctc cgtcttgtat
     4801 gtataaagtg cggcctgata tttactctgg atttcctcct gtttctttgc ctctgcctgg
     4861 gcttttgcct gagcttcctg ctgctgtgcc tgagcctgag ctgctgcctg tgcggatgcc
     4921 tgagctgccg cttcacgctg tgccgctgct gctgccgctg aaatctgtga tttcagagac
     4981 gagacataag cttttttatc ctttattttc ttctcaagct cctgaacatt ctttttggca
     5041 tccttctgct tcttctctgc tttgtaagat ttatttcttg tatcctttgt atcatacttt
     5101 gttgatttca gattcagttc tgctttccga ttatctttct ccagatcctc tgtattaaac
     5161 tcgatcagtt ttgttccctt tttcaccaca tctccctggg caaaagaaac cgttttcagc
     5221 tctgcattta caggagaata ataggtcttc tcctcttcac ttcccactgt tcctgtagca
     5281 tctacaattt cctcaacatc acccttctct gcaattacca cctgaacctg cgggatctga
     5341 ttttctgtgg aattcctctg acctgctact gtaactccgc cggcaacaac gagaacagca
     5401 gctgctccaa taataatttt ctttttactt ttcttttttt tcataatatt tgtctccttg
     5461 taaccgggtt tatgcatcca gctgattttt tatatattct tctttcccat agtttttatt
     5521 gataaaatct gactctatct ttccatccat aattctgact acacgccttg cctgagcagc
     5581 aatcccatta tcatgtgtga tcaatatgac cgtttttccc tgctcgtgca tacttttaag
     5641 aatctgaaga atctccttac tggatgcaga atccagattt cctgtaggtt catctgccag
     5701 gatcaccggt ggctgggccg cgattgctct tgcaatagcc accctctgct gctgtcctcc
     5761 tgacatttca tttggtctgt gatccatacg tttctcaagc cctaccatgg tcagtgactc
     5821 tactgccagc ttatgtctcg tttttctgtc tacccctctg taaaccagcg gtagttccac
     5881 attttcaatt gcagtcagat ttgagatcag attaaatccc tgaaaaataa atccgatctc
     5941 ccggtttctg acttctgaga gttcattgtc ttccattgtg gacacatcag ttccattcag
     6001 aaaatattcg ccggatgtag gcacatccag acaacccagc atattcataa atgtagactt
     6061 tcctgaacct gactgtccga taatagcaac aaactcaccc ttattaattt ccagattaat
     6121 atgatccagc gcccgtactt cattctcgcc gggattatat attttacaca aatcttttac
     6181 tctgatcagc gcttcctcca tctttccctc ccgtctatta aatatcctgc tccaatccag
     6241 atcttacaga atcgcaacct gtctttcgta agatatcaga aatacctttt acaatcataa
     6301 cctcacttat atgttaaaag ggcagcgctt cagttttcca aaagctctgc ccttatctgc
     6361 ggaatctgca agtctctgtt atcagaaacg atacagcttc cacgatttct tcatattttt
     6421 attccgcatc tgcctgactt tttatgcttc cagcagaaat gccttatatg ctttcttagc
     6481 ttcgtaaata tctgccttac ttgtcacttc ccatggtgca tgcatactga gcactgccac
     6541 accgctgtca atgacttcca ttccataaag tgctgcgata taagcgattg ttccgcctcc
     6601 gcctacatca actttaccaa gctcagctgt ctggaacgca acttcatggc tgtcaaaaat
     6661 atttctgact cttgccacat attctgcatt tgcatcattc gagccggatt tacctcttgc
     6721 tccggtaaat ttatttaata cgatacctct tccaagataa gctgcattct ttttctcaaa
     6781 tgcctcccca taagccggat catagcctgc acttacatca gaggaaagca tactggaatt
     6841 cttcagagtt cttctcaatc tcagatcact gtagcatccc attgcatcca gaagttcagc
     6901 cactgcgttc tcaaagaaac gtgactgcat accggttgca cctacgcttc caatctcttc
     6961 tttatcagta aacagacagc aggaagtgtg tttcggggtg tccatcccaa gcattgcaag
     7021 cagagaagta tatgcgcaca ctctgtcatc ctgtccataa gcagcgatca tacttctgtc
     7081 cagaccacac tctcttgcct ttcctgccgg tacgatctcc agctctgcag aaagaaaatc
     7141 ttcctcttcc atatcatact tctctttaag gatattcagt acattctgtt ttactgcctc
     7201 tttcttctta tcatcttcca ggtctttcag tggtctgctg ccgatcagga tgtccagatt
     7261 ctctccctcg atcacttctg ccgctttttt ctgcatctgc tttcctgcaa gatggatcag
     7321 aaggtctgtg atatatacga tcggatcatc ctcatcctca ccgatattta cttctgctgt
     7381 acttccgtct ttctttacca ctacgccatg aattgccagg ggaagagtca cccactggta
     7441 tttcttaaca ccgccataat aatgggtatc cagatatgca agatccgtat cctcatataa
     7501 tggattctgt ttgacatcaa gacgtggaga atcaatgtgt gctcccagaa tattcattcc
     7561 ctcagaaata tcatcctgtc cgatatgaaa cagtgcaatg atcttcttca ttccgactgt
     7621 ataaacttta tctcctgctt tcagtttctc acccttcgca gtcttctctt ccagagattc
     7681 ataacctgct gcccgtgctt ctctgatgat ctgcgtcaca cattcacgct ctgtttttcc
     7741 cacattaaga aaattgcgat acgcttttgc aacctgctcc agttcttttt cttctgcttc
     7801 tgtataagaa agccatgcat ttctacgttc catttactgt tccgcctttc tttatgactg
     7861 tcattcatag tattgctttg ataacggtaa ctattcatca gttcgctatt atgtaatgat
     7921 attgaacaat tacctgtttt tcattataaa acatcctctg aaaattttcc atatgattaa
     7981 tatttatttg tcagtggaca ttatgattca tttccgctat agttgatggc aggttaatgt
     8041 ttctcctctc aaattcatta tttgcgagga ttattataaa aatatctgca ataccaggag
     8101 gttctgtcat gaaaaattca gaggaactgc gtcagcagct tcgttccata aaccgcaaaa
     8161 gttatccggc atataaaggg ctgaaaggtc tgtatcattt tggaaattat attctgtcca
     8221 tcgaccatgt gcagggagat ccttttgctt ctccctctca tgtcagcatt cagatttcac
     8281 acagagatac cggatttccc gtcgaatatt ataaagatac acttaccggg actactcttt
     8341 gcgactatct tacacgtcag tttgaaaaac aggtcagtca gtacagcttt cgtgcaaaag
     8401 gctccggcaa aagcggcctg ctcactgcca gtcattgtgg tcaggagatt ctttcccgga
     8461 cagcctgtga aataacagaa aaaggcataa ccgcccgctt ttttgtgggc ttccctgcta
     8521 atggccgcac gatcaatgcc actgaactgg agaaaatcct ttttgatttt ctgcctgtat
     8581 gcattcaaaa atcctttttc tactccagcc ttaatgcaaa ggaattacag aattatatag
     8641 agcttgcaga ggatcaggaa tttatccgtc agacgctgcc cgcaaaaaat ctctgcgcat
     8701 tcatagccga tggctccatc ctcccaagag aaagcggtat ttcctcccgc cctatgaaag
     8761 cttccattcc ctttacttct ccggacagtc tgcgcatttc cataaatctt ccccataaag
     8821 gaaaaatcac aggcatgggg attccaaagg gaatcactct gatcgtaggc ggtggatatc
     8881 atggcaaatc cacgcttctg aatgcacttg aacttggtgt atacaatcat atccccggag
     8941 atggacgtga atacgtgatc acagatgcaa ctgctgtcaa actgcgttca gaagatggcc
     9001 gttttatcaa ggatgtggat atttccatgt ttataaatga tcttccaaac aagaaagata
     9061 cccgctgttt ctctacactg gatgccagtg gaagcacttc ccaggcagcc ggtatcgtag
     9121 aaagcatgga agccggaagc cacctttttc ttcttgacga agacacctcc gcaactaact
     9181 tcatggttcg tgatgctttc atgcaacagg ttatccagcg tgagaaagag ccgatcactc
     9241 cgtttctgga acgtgcagaa gatctttata aaaaagcagg gatttctaca attcttgttg
     9301 ccggtagttc cggggcattc tttcatattg cagatacaat tatccagatg gacaattacg
     9361 ttccaaaaga tattacagct tctgtcaaaa aattatgcag ccaatatcct ctgcctgctg
     9421 tttcagttac tgattttcag cttccgcaca gccatcgcat tatgagcaga cctgccgaat
     9481 cttctaagcg cctcatacat aacagtcgtg gaaatcattc cgattccggt gccgcaaaac
     9541 ctgagcgcct gaaaaccaga atctccggaa cagatggctt ctctctcggc agacaggaga
     9601 ttgatctccg ctatacagaa cagcttattg acgcagaaca gaccgctgcc ttaggtctcc
     9661 tgctgaaata tgcagttgaa catctggcag acggcagacg taccctgcct gagattgtgc
     9721 agtttctttg gaaaaatctc tctttacatg gtctgtcctt ctttacggag aatcaaaaaa
     9781 tttcctgtgg ttacgctact ccccgtattc aggaaattta tgcctgtctt aatcgttaca
     9841 gaggtttata agaaagaatc ctgcttcagt gacataataa aatcaccgct tctccgtcga
     9901 tcttgtcggc taagcggtga ttcacgttca ttttacactt tttagggaaa ggaaagattt
     9961 tctcttaagg tacgtcattt ccttaagatg taaatatcat aacctataaa tgtgagagaa
    10021 atttgtctaa tttctaatat aattataaaa tttataaaaa aactgcaaaa atttgttact
    10081 gttcactccg ttctcagcaa cacaaaaatt ttacactttt agaaattttg taggaatcag
    10141 ttgcatctcc ctttcaatcc gttataatat tagatgtatg taactattta ataatcaaag
    10201 aaatctctgc ggaacagtta tctgatatag agctgcataa cacaacaaag gttagtacac
    10261 cgttttcgaa ataactatac cactcacttg tctgcgaatc cgattggtat agttatttac
    10321 gaaacgatgt acctggaaag aggaaaataa atgtttagaa aaatagataa aaccagtaca
    10381 ttcaacgatg ttctgaaaca aattatcgaa aatatccaaa acggagaatt acaacccggc
    10441 gacgcacttc ctgctgaacg tattctggca gaagaattcg gaatctccag accagcatta
    10501 cgagaagtcc tgaaggctct ttccctgctc gggattactg tcagcgtaca tggcggtgcc
    10561 aattatattg caactgatct tcacagctgt cttactgaac ctctgtctat catatttcaa
    10621 atgtacaaca gcaaaattca ggatgcattg cagcttcgag gcgcactgga aagcaaagct
    10681 gcttttctcg ctgcccaaaa ctgtacaccg ctggatgctg ctgaactcca gcttatcatt
    10741 gccagactgg acagcactca ggatgaaaaa atccgcggtg atctggatcg tgatctgcat
    10801 ctgaaaattg ctcagatttc tgacaatcca ttaatactga gtgtcttaaa tgcctctgca
    10861 cagattaccg aaaatatgat cgttggaata cgttcctatc ttatgcagaa aaataacgat
    10921 gcaccggaga ttgattccca gcacactcgg cttgttcagg caataaccaa taatgatgca
    10981 cctcttgcag aaaaagttat gaacgaacat atgcatacta tagaatatct cctgaatgaa
    11041 attacgtctg acaacctcac aatttctgat aacgttcaac aaccataaat taccctgatc
    11101 atgttatcaa aaattatatg tcagacccac ttatcaaacc tatggcaatc ctctaaaatc
    11161 atactttatc tggagaattg ccatatctgc tttacaatca ttctgtttct tcactgaaaa
    11221 tctcgcacat cagccttgcc cctgttttaa atcccatggc aaaactttct tccagctctt
    11281 gtggatatat ttccatatgt ttattcatca gttcatccag ttcttcagca agctcatctg
    11341 tcagttgaac tctcaatttc tgatagcact ggtatgcctt ctcttttttc tctttacata
    11401 aatcatcatg cattactgac atttcatttt tttctctatt gtcattatcc atatacatac
    11461 catacaattt ccgaatcaca ttttccatat atttacccag tctctctgaa aatatttatt
    11521 tactgcattc tagagcgtgt ctgaaaaagg ctttctgcaa gatgtgcgtc acaatttgtg
    11581 ggatattttg cccggatgag ggcgccgtag cgggctacgg caactgaatt cgggtgaaat
    11641 atcccgcaaa gtggggcgtg cagattgcgg gaatgatttt tcagacacac tctaatgtta
    11701 caagtattcc ccacatattc ataactcact catactatcg catcaaatta attcttacaa
    11761 cagtctcgca ggataacctt tctatttcaa agcggagaaa agtaatacta atatgtatta
    11821 taaatttgtg ttatatcaat gtcaatcctg tgtaactata ctttatatca taacagtgat
    11881 tacggagatc aaaattatag caatcctcgt aaataatgca tttaagacag ttcagcagag
    11941 tgcgaaagac aaggaagata tctgcaggcg tgctgacgca cggcaaagat atctgacgca
    12001 ggatttcaca ctctgatggg ctgg
//
LOCUS       DBMDXG010000012        11852 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1549_length_11852_cov_1.842567, whole genome shotgun sequence.
ACCESSION   DBMDXG010000012 DBMDXG010000000
VERSION     DBMDXG010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1549_length_11852_cov_1.842567"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="ACZPD3_00890"
     CDS             <1..938
                     /locus_tag="ACZPD3_00890"
                     /inference="COORDINATES: protein motif:HMM:NF022633.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HIR1414607.1"
                     /translation="MIVYHYCSLESLNSILKNRSLRLTNILKSNDSMEISWICRYYDT
                     EFKRAYENASDLFRSKISSERLMGYVKLFTDEFFNENHADFRYYVTCFSYQNDLLSQW
                     RGYADDGRGAAIGFDLDVLKEVVTVSPEISKPSIVSLHKISYSETEQREVVHQIVHEL
                     VDEIEKILQKEEQCRESIEKKQDYEIEVLDKVMNCFEKKFLKLFQESVYMKNPFFREE
                     SEIRLCEFSPKQFLMGREVELSLGARLYNYSYYVKESQLISYVDFDFSDCLDQLIKEL
                     VIGPKCLMSERDMEYYLTTLGLSNCRVKKSQGTYR"
     gene            1001..2329
                     /locus_tag="ACZPD3_00895"
     CDS             1001..2329
                     /locus_tag="ACZPD3_00895"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF021507.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR1414608.1"
                     /translation="MKRKLLFRKVAVFLILALFLTAGISMRAVTVNAATYVKQDRTSV
                     SITSKKTGWQKINGDYYFYNSKGRLICGSFKYKGYYYYSIANGKRFTGWMKRSGNKYY
                     YNRKNGAMFRNRWATGDKYTYYFNKSGVAIARQWLTQDGKKYYFLSNSTMAKGWQKIG
                     KYYYYFSKKTGVMAKNTWIGKYYVNSKGQRVKSSDTKPTNTKPTVTQSGNTYEYKSST
                     LNIKLERKSVHGISYWAAHIKTSNAKQLKSALSNGTYGGSRQTTSDAVSSNGGIIGVN
                     GSAFDYGTGKPSPLGMCIKNGIIYGDYMTSYSVMAVKKDGTIYTPAQGLMGKNLLAAG
                     VKDTYNFGPVLIKDGEAQLPWAETEKYYPRTAVGMVKPNDYVLLVTDTGSYNGLNHWD
                     MVDIFKSYGCTYAYNLDGGGSATLYFNGKVMNKLIGNTQRPCADFLYFTR"
     gene            complement(2314..2460)
                     /locus_tag="ACZPD3_00900"
     CDS             complement(2314..2460)
                     /locus_tag="ACZPD3_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414609.1"
                     /translation="MSMFIHAIIVILLAVLAMWIGLLILVCWALAACASRRDSYLEKD
                     YRVK"
     gene            2972..4261
                     /locus_tag="ACZPD3_00905"
     CDS             2972..4261
                     /locus_tag="ACZPD3_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR1414610.1"
                     /translation="MKKYSDMSKEELLTLKAQLDKEYADIKAKGLALDMSRGKPSADQ
                     LDLSMGLMNVLSSDSDLKCETGVDCRNYGVIDGIPEAKRLLGEMSEVDPDHIIIYGNS
                     SLNVMFDSIARSMTQGVMGNTPWCKLDKVKFLCPVPGYDRHFKITEFFGIEMINVPMT
                     PQGPDMDMVEKLVSEDASIKGIWCVPKYSNPQGYTYSAETVERFAHLKPAAPDFRIYW
                     DNAYSIHHLYDDNQDFLVEILEACAKAGNPDLVYKFTSTSKVSFPGSGIAAVAASKAN
                     LEDFRSYMQIQTIGHDKLNQLRHVKFFKNIDGLHEHMRKHAAILRPKFEMVLNTLDRE
                     LGGLGIGEWTKPHGGYFISFDSMEGCAKAIVAKAKEAGVVLTGAGATYPYGKDPKDSN
                     IRIAPSFPTPEDLGKAAEVFVLCVKLTSVEKLLATVR"
     gene            4520..5899
                     /locus_tag="ACZPD3_00910"
     CDS             4520..5899
                     /locus_tag="ACZPD3_00910"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1414611.1"
                     /translation="MSSRKKIMITLIIILLLLTAGVYGYGVYYFTEHFLPGSMVNGFN
                     CSYMTVSQSEELLTQKVGAYVLTIDTRNNGQESITAKQAGLSYDSDGSVDKLIRNQDR
                     FTWFLAFNQHRNYEVSSSIKYDEQKTEAAISELKCMQQENMTEPSDAHIEEKDDKFVI
                     VPEQEGTALKPEKTSQDIIDALVTGRTPVDLEADGCYKEPKVYQSDETLTKNCELINK
                     LTDVVITYDFDDCTETVDRDMIKNWLTTDENGLYTLDKKQIEAYISELAAKYDTVGTE
                     RTFNTYDGREITVSGGNYGWQIDQKAELKELTELIKNGETQVREPVYSHEGLVRKTND
                     IGYTYIEIDLTAQRMVFYKDGTPTADAQIVSGNPFVPNCATPVGCYTTGEMKSGCTVN
                     GEDYPSAVNYWIPFDGNLGISDAPWRMDFGGQLYEFEGTHGSICAPSDQVQIIFSNVE
                     KNTPIVIYE"
     gene            5912..6964
                     /locus_tag="ACZPD3_00915"
     CDS             5912..6964
                     /locus_tag="ACZPD3_00915"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR1414612.1"
                     /translation="MKIVVLAGGTSTERTVSITSGTGICKALRQKGHQAILVDIFCGI
                     ENVDRENPFPSEYDVDAASEYMSAFNDRIEQMKKERRSFFGPNVLKLCEAADIVFLGL
                     HGANGEDGKVQATFDLMGIKYTGTGYLSSALAMDKGITKQMFLMNNVPTPRGVSMVKE
                     EMTTDLKALGMEFPVVVKTCCGGSSVGVYIVNDQAEYEQALKDAYSYENEVVVEEYIK
                     GREFSVAVVDGKAYPIIEIAPLQGFYDYKNKYQAGSTIETCPAEISPELTEKMQHYAE
                     AGAKALFMEGYCRLDFMMKENGDMYCLEANTLPGMTPTSLIPQEAKVLGIDYPTLCEK
                     LIEVSMKKYPTQDFTL"
     gene            7050..8420
                     /locus_tag="ACZPD3_00920"
     CDS             7050..8420
                     /locus_tag="ACZPD3_00920"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR1414613.1"
                     /translation="MKNLTLENIARVCGGTYYGPADKLQEEVMSVITDSRKAEEGCLF
                     VPIVGERVDAHKFIPQVMEAGALATLSERVLDNADFPYIAVESSLQAVKDIAEFYLKQ
                     LQIPVVGITGSVGKTSTKEVIASVLNQKYHTLKTQGNFNNELGLPLTVFRLRDGDEIA
                     VLEMGISDFGEMTRLAQIARPDTCVITNIGTCHLENLGDRNGVLKAKTEIFKFLRPDG
                     HIVLNGDDDKLITVKEYEKIKPVFFGMSNECQVYGDKIVSRGLKGMTCTIHLGDTAFT
                     VDIPMPGRHMVYNALAAAAVGNIYGLTTEQIKAGIESLEPISGRFRMIETDKFLIVDD
                     CYNANPMSMKASLDVLQDGAGRRIAVLGDMGELGENEVQLHEEVGEHAGKCDIDVLIC
                     TGKLCRNMAERAQRTNPDLKVIYEPDRESLLEHLEGYVQDGDTILVKASHFMKFEEVV
                     TKLENM"
     gene            8542..9138
                     /locus_tag="ACZPD3_00925"
     CDS             8542..9138
                     /locus_tag="ACZPD3_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414614.1"
                     /translation="MLEKVMELHFLLYAMMLMGGLGALGMLTTHLTYRRMIRKNTEVK
                     SNLKEKWTNLWKTRDRLLNRMNRFVWYPSLFCIAFLGLAFFLYSKDARWDGMPLAYLY
                     VAAIIPTALLLLRQALDFTYREELLMNSFSDYVEHARTWVEEVPVPEKADEAVKEEIV
                     EHIADSIRQTAAAGSHFSGMLTPEEDEIMREIIREFMK"
     gene            complement(9238..9510)
                     /locus_tag="ACZPD3_00930"
     CDS             complement(9238..9510)
                     /locus_tag="ACZPD3_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414615.1"
                     /translation="MFRLWGKIWKDNHLLKDTVICDDSEDTRTHKIFRGLESICYEMD
                     LGNPIWLESTVRHFKKHGKARFYQDNFIEQIDFDYLEIQVIEEDDE"
     gene            9835..>11852
                     /locus_tag="ACZPD3_00935"
     CDS             9835..>11852
                     /locus_tag="ACZPD3_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR1414616.1"
                     /translation="MKLYDTGVYLLNGQKIVPENQADFPVSKEEAAKSTIAYSILKAH
                     NTSGNMEKLQIKFDKLTSHDITFVGIIQTARASGLEKFPVPYVLTNCHNSLCAVGGTI
                     NEDDHMFGLTCAKKYGGVYVPPHQAVIHQFAREMLAAGGKMILGSDSHTRYGALGTMA
                     MGEGGPELVKQLLSQTYDINMPGVVAIYLTGSPRPGVGPQDVALAIIGKVFANGYVKN
                     KVMEFVGPGVANLSADFRIGVDVMTTETTCLSSIWQTDEKIQEFYEIHGRSEDYKELN
                     PGAVTYYDGMVYVNLSEIRPMIAMPFHPSNVYTIDDVRKNLKDVLHDVEQKALVSLDG
                     AVDYSLQDKVIDGKLYVDQGIIAGCAGGGFENICAAADIIKGHYIGSDEFTFSVYPAS
                     TPIYMELVKNGAVADLMEAGTIVKTAFCGPCFGAGDTPANNAFSIRHSTRNFPNREGS
                     KLQSGQIASVALMDARSIAATAANKGFLTPATDMDVEYKGQKYHFDQKIYANRVFDSH
                     GVADPDTKIKFGPNIKDWPAMSALPENLVLKVVSEIHDPVTTTDELIPSGETSSYRSN
                     PLGLAEFALSRKDPAYVGRAKEVQKAQKAIEAGECPLEVLEELKPVMAKIQEKYPEAG
                     KGNIGVGSTIFAVKPGDGSAREQAASCQKVLGGWANIANEYATKRYRS"
BASE COUNT         3833 a         2194 c         2761 g         3064 t
ORIGIN      
        1 atatgatcgt atatcactat tgcagtttag aatcattgaa ttctattttg aaaaatcgtt
       61 ccctccgtct gacaaatatc ctgaaatcca atgattccat ggagatcagc tggatctgca
      121 ggtattatga cacagaattt aaaagagctt atgaaaatgc ttcagattta ttcagatcaa
      181 agatttcatc agagagattg atgggatatg tgaaactgtt tacagatgaa tttttcaatg
      241 aaaatcatgc agacttcaga tattatgtaa cctgtttttc gtatcagaat gatctgctca
      301 gtcagtggcg gggatatgcg gatgacgggc gcggagctgc catcggattt gacctggatg
      361 tattaaaaga agttgtaacg gtttctccgg agatttcaaa gccgtcaatt gtgtcattac
      421 ataagatcag ttattctgaa acagagcaga gagaagttgt acatcagata gtgcatgaat
      481 tggttgatga aatcgagaag atattacaga aagaagaaca atgtcgggaa agcattgaga
      541 agaagcagga ttatgaaata gaagtactgg ataaagtcat gaactgtttt gagaaaaagt
      601 ttttgaaatt atttcaggaa tctgtttaca tgaaaaatcc gtttttcaga gaagaaagcg
      661 aaatccgatt gtgcgagttt tcgccgaaac agtttctgat gggaagagaa gttgaacttt
      721 ctctcggtgc acgtttatat aactattcct actatgtaaa agaaagtcag ctgatatctt
      781 atgtggattt tgatttttca gattgccttg accagttgat aaaggaactc gttatagggc
      841 caaagtgtct gatgtcagag agagacatgg aatattatct gacaacgctc ggtctgtcga
      901 attgtcgggt aaagaaatcg caggggacat atcggtaata tatttatagc aattccctta
      961 tatgggcata tcagacatcg tggaaaggca gatgaaagac atgaaaagaa aattactatt
     1021 cagaaaagtg gcggtatttc tgatactggc attatttctg acagcaggca tcagtatgcg
     1081 tgcagttacc gtaaatgcag ctacatatgt gaaacaggac agaacctccg tcagcatcac
     1141 atcgaagaaa acaggatggc agaaaataaa cggagattac tatttttata attcaaaggg
     1201 gcgtctgatc tgcggttctt ttaagtataa aggttattat tactacagta ttgccaacgg
     1261 caaacgtttc accgggtgga tgaagcgttc cggtaataaa tactattaca atcgtaaaaa
     1321 tggtgccatg ttccgtaatc gctgggctac aggagataaa tatacctatt actttaataa
     1381 atccggcgtg gcaattgcca ggcagtggct tactcaggat ggaaaaaaat actatttcct
     1441 cagcaattcc accatggcaa agggctggca gaagatagga aaatattatt actatttcag
     1501 caagaaaaca ggagtgatgg caaaaaatac ctggataggt aaatattatg taaacagcaa
     1561 agggcagcgt gtaaaatctt cagatactaa accgacaaat actaaaccaa cagttactca
     1621 aagcggcaat acttacgaat acaaaagcag cactcttaat attaaacttg aacgcaaatc
     1681 agtccatgga atttcctact gggcagcgca tataaagaca tccaatgcca aacagctgaa
     1741 atccgccctc tccaacggaa cttatggcgg atcccgacag acaacttctg atgcagtatc
     1801 ctcaaatgga ggcattatcg gagtaaacgg cagtgctttc gactatggta caggaaaacc
     1861 atcccccctt ggcatgtgca tcaaaaacgg gattatctat ggtgattaca tgaccagtta
     1921 ttcagtcatg gcagtaaaga aagacggaac catctatact ccggcacagg gacttatggg
     1981 aaaaaatctt cttgcagccg gtgtaaaaga tacatataat tttggtccgg ttcttattaa
     2041 agatggtgaa gcccagcttc catgggctga aacagaaaaa tattatccac gtacagcggt
     2101 aggaatggta aagcccaacg attatgtact ccttgtaaca gataccggca gctataatgg
     2161 tcttaatcac tgggatatgg tggatatttt caaatcctac ggctgtactt atgcttacaa
     2221 cctggatgga ggcggttctg caaccctgta ctttaatgga aaagtaatga ataaacttat
     2281 cggcaatact cagcgtccat gcgcagactt cctttatttc acccgataat ccttttccag
     2341 ataagaatcc cgtctgcttg cacatgctgc caaagcccag cacacaagaa tcagcagccc
     2401 gatccacata gccagcacag caagcaggat tacgataatt gcgtggataa acatagacat
     2461 ggatgacctc ctttcacagt cattcggtta tgaactgagc agcgaagcag atgattttat
     2521 ccgcctgact tgtctcattc actgacttgg ataaaaatgg atgttgtata aggagtaata
     2581 aaaagatatc aggatgacct gacaagaaca attaagacag ttaaaagatc tgttaactga
     2641 tttaaatata cgctgtcaca ctcaaaaaag caagccgaaa ctttcgaggg atttcgactt
     2701 gctttttggc taattattta cgaggcttgc tataagtatc agagaaagat gttacggcaa
     2761 ttcggaatga atttgggtta cgttactgag aacagaggga acagtaacga ttgttttggt
     2821 aaaatattaa caattccata aaaacacttg acaaccctac tttaccatac taaaatgtaa
     2881 aaagcaaaaa aactgttgcc gcaggaaaaa caaatgattg tcctatacgg aaaaagggac
     2941 atccttccat ggcagaacaa ggaggaatat tatgaaaaaa tacagcgaca tgagcaaaga
     3001 agaactgctt acactcaaag cacagctgga taaagaatac gcagatatta aagcaaaggg
     3061 cttagctctc gatatgtcac gtggaaaacc gtcagcagac cagctcgatc tctcaatggg
     3121 attaatgaac gtattaagca gcgattcaga cctcaagtgc gaaacaggag tagactgccg
     3181 taactacggt gttatcgacg gaatcccgga agctaaacgc ctccttggag aaatgtccga
     3241 agtagatccg gaccacatca tcatctacgg aaactcaagc ttaaacgtta tgtttgacag
     3301 cattgcacgt tctatgacac agggtgtaat gggaaatacc ccatggtgca aacttgataa
     3361 ggtaaaattc ctctgtcctg taccgggata tgaccgccat ttcaagatca cagaattctt
     3421 cggaatcgaa atgatcaacg tgccaatgac accacaggga ccggacatgg atatggtaga
     3481 gaagcttgtc agtgaagacg catccatcaa aggaatctgg tgtgtgccga aatactccaa
     3541 cccacaggga tatacttatt cagcagaaac agtagagcgc tttgcgcatc tgaaaccggc
     3601 agcacctgat ttccgtatct actgggataa cgcatacagc attcatcatc tctatgatga
     3661 caatcaggac ttccttgtag aaatccttga agcatgtgca aaagcaggaa atcctgatct
     3721 tgtatataaa tttacatcaa catcaaaagt cagcttcccg ggttccggta ttgcagcagt
     3781 tgcagcttcc aaagcaaacc tggaagattt ccgcagctac atgcagatcc agacaatcgg
     3841 acatgacaaa ttaaaccagt tacgtcatgt aaaattcttc aaaaatatcg acggtcttca
     3901 tgaacacatg agaaaacatg cggcaattct ccgtccgaaa tttgaaatgg ttcttaacac
     3961 actggacaga gaacttggcg gtcttggaat cggcgagtgg acaaaaccac atggtggata
     4021 cttcatttcc tttgattcaa tggaaggatg tgcaaaagct attgttgcca aggcaaaaga
     4081 agcaggtgtt gtacttacag gagcaggcgc aacttatcca tatggaaaag atccgaaaga
     4141 ctccaatatc cgtattgcgc caagcttccc gactcctgag gatcttggaa aagcagcaga
     4201 agtattcgta ctctgtgtta aacttacaag tgtagagaaa ctactggcta ctgttcgctg
     4261 ataatatccc gcgaggtgtt ttttgtgagc gaagatcaca gaaaacccga gacataccgc
     4321 gcgaatttat gcaattagca taaattctgt gcatcgcgaa gcgtgttact gtgaacggag
     4381 tgaacagtaa cactactgga aaacgcataa aagagaataa tcgtatttac tttctggata
     4441 aataatgtga taataaaact gcccggcata atttcttgcc gggcggttgc tgtttataaa
     4501 aaagaaagga gaggatgtcg tgagcagcag aaagaaaatc atgatcaccc tgatcataat
     4561 cttgctcctg ctgactgccg gagtatatgg atatggtgta tactatttca cagagcattt
     4621 cttaccggga tcaatggtaa acggattcaa ctgttcctat atgacagtat cccagtcaga
     4681 agaattgctt acgcagaagg tgggagcata tgttcttact attgacacca gaaataacgg
     4741 acaggaaagt atcacagcaa aacaggcggg actttcctat gactcagatg gaagtgttga
     4801 taaactgatc agaaatcagg atcgttttac atggtttctt gcttttaatc agcacaggaa
     4861 ttatgaagtg tcctcctcca ttaaatatga tgagcagaag acagaggctg ccatttccga
     4921 actgaaatgt atgcaacagg agaatatgac agagccttcg gatgctcata tagaagagaa
     4981 agacgataag tttgtaattg ttcctgagca ggagggaact gcactgaaac cagaaaaaac
     5041 aagtcaggat attatagatg ctcttgtcac aggacgaacg ccggttgatc tggaagcaga
     5101 tggatgctac aaagaaccga aagtttatca gagtgatgaa actctgacga agaattgtga
     5161 actgataaac aaacttacgg atgtagtgat aacctatgat tttgacgatt gcacagagac
     5221 agtagacaga gatatgatca aaaactggct cacaacagat gaaaacggtc tttatacact
     5281 tgacaaaaaa cagatagaag catatatcag tgaactggcg gctaaatatg atacggttgg
     5341 aacagagaga acatttaata catatgatgg tcgtgagatt actgtcagcg gagggaatta
     5401 tggctggcag atcgatcaga aagcggagct gaaagaactt acagaactga taaaaaacgg
     5461 agaaacccag gtcagagaac ctgtttattc ccatgaaggt ctggtcagga aaacaaatga
     5521 tatcggatat acgtatatag agattgacct gacggcccag agaatggtat tctataaaga
     5581 tggaacgccg actgcggatg cgcagatcgt atccggaaat ccattcgtgc ccaactgtgc
     5641 aactcctgtg ggatgttata caacaggtga aatgaagagc ggatgtacag tgaacggaga
     5701 ggactatcct tctgctgtaa attactggat cccattcgat ggaaatctgg ggatcagtga
     5761 tgctccatgg agaatggatt tcggaggaca gctttatgaa tttgaaggca cacatggaag
     5821 catctgtgca ccatccgatc aggtgcagat cattttcagc aatgttgaga agaacacacc
     5881 gattgttatt tatgaataaa ggagaaaaat gatgaagatt gtagtattag cggggggaac
     5941 aagtacagaa cgtactgtct ccattacatc aggaacagga atatgtaagg cattacgaca
     6001 gaaaggacat caggcgattc ttgtggatat tttttgcggc attgagaatg tggaccggga
     6061 gaatccgttt ccgtcagagt atgatgtgga cgcagcatca gaatatatgt cagcatttaa
     6121 cgacaggatt gaacagatga aaaaagagag aagaagcttc ttcggaccaa acgtgctgaa
     6181 actctgcgag gcggctgaca ttgttttcct gggacttcat ggtgcgaacg gagaagatgg
     6241 aaaagtacag gcaacatttg acctgatggg aatcaaatat acaggaaccg gatatttaag
     6301 cagtgccctg gcaatggata agggaatcac caagcagatg ttcctgatga ataatgtacc
     6361 aacaccgcgg ggagtgtcta tggtaaaaga agaaatgacc acagatctga aagcgcttgg
     6421 aatggaattt cctgtagttg taaaaacctg ctgtggcggt tccagtgtag gtgtttatat
     6481 tgtgaatgat caggccgaat atgagcaggc tctcaaagac gcttattctt atgaaaatga
     6541 agtcgtggtc gaagaatata taaaaggacg tgaattttca gtagcggttg tggacggaaa
     6601 agcttatccg atcatcgaaa ttgctccgct tcagggattc tatgattata agaataaata
     6661 tcaggccggc tctacgattg agacctgtcc tgccgaaatt tcaccggaac tcacagagaa
     6721 aatgcagcat tatgcggaag caggagccaa agctctgttt atggaaggat actgccgtct
     6781 tgatttcatg atgaaggaga atggagatat gtattgtctg gaagcaaata ctcttcccgg
     6841 aatgacacca accagtctga ttccgcagga ggcaaaagtt ctgggaatcg actatccgac
     6901 tttatgtgag aaactgattg aagtttccat gaaaaaatat ccgacgcagg atttcacatt
     6961 atagagcgtg tctgcagtaa tttatagaat tgttatagat gcattattaa gagaattgct
     7021 ataacaggaa agaagatata aaaaatatta tgaaaaactt aactcttgaa aatatcgccc
     7081 gcgtatgcgg cggtacttat tatggtccgg ctgataaatt acaggaagaa gtaatgtctg
     7141 ttatcacaga cagcagaaaa gcagaagaag gatgtctgtt tgtacctatt gtgggggaac
     7201 gtgtagatgc ccataaattt atcccacagg taatggaagc aggggcactg gcaaccttgt
     7261 cggaacgggt gcttgataac gctgattttc cttatatcgc agtagaatct tctcttcagg
     7321 cagtgaaaga tattgcagag ttttatttaa agcagcttca gatcccggtt gtgggaatta
     7381 caggaagtgt tggtaagaca agtacaaaag aagtgatcgc atctgttctg aaccagaaat
     7441 accatactct gaaaacacag ggcaacttta acaatgaact tggtttgcct ctcacagttt
     7501 tccgtctgcg tgatggagat gaaattgcag ttctggaaat ggggatcagt gatttcggtg
     7561 agatgaccag acttgcgcag attgcaagac cggacacctg tgtgatcacg aatatcggta
     7621 cctgccatct ggagaatctg ggagacagaa atggtgttct gaaagcaaag acagagattt
     7681 ttaaattcct cagaccggat ggacatattg tattaaatgg cgatgatgac aagcttatca
     7741 cagtaaaaga atatgaaaag ataaaacctg tatttttcgg aatgagtaat gaatgtcagg
     7801 tttacggtga taagattgta agcagaggtt taaagggaat gacctgcacc attcatctgg
     7861 gggatactgc atttacagtt gatatcccga tgccggggcg ccatatggta tataatgcgc
     7921 tggcagcagc tgcagttgga aatatttatg gacttacaac tgagcagatc aaagccggaa
     7981 tcgagagcct ggagccgatc agcggtcgtt tcagaatgat cgaaacagat aaatttctga
     8041 tcgtagatga ctgctataat gcaaatccca tgtccatgaa agcctctctg gatgttctgc
     8101 aggatggtgc aggacgcaga atagcagtcc ttggcgatat gggtgaactt ggagagaatg
     8161 aagttcagct tcatgaggaa gtaggagagc atgccggaaa atgtgatatt gatgttctta
     8221 tctgtacagg taaactgtgc agaaatatgg cagagagagc acagcggaca aatccggacc
     8281 tgaaagtaat ctatgagcca gacagagaaa gtctgctgga gcatctggaa ggttatgtac
     8341 aggatggtga cactattctt gtgaaagctt cccattttat gaagtttgaa gaggtagtta
     8401 cgaaactgga aaatatgtaa acacttttgt ctttttatga gagaatttga tgagcgattt
     8461 tagttccatg ataccggaaa cagcagtata ataatcctgt cagtttatga gcaggcagcg
     8521 tgttcaagtg aggaggatgt tatgctggag aaagttatgg aactgcattt cctgctgtat
     8581 gccatgatgt taatgggcgg actgggagca ctggggatgc tgaccacaca tcttacctac
     8641 cgaagaatga tcagaaaaaa tacagaggtt aaatcaaatc tcaaagagaa atggacaaat
     8701 ctttggaaaa caagagacag gctgttgaac cgtatgaacc ggtttgtctg gtatccgtca
     8761 cttttctgta ttgcttttct tggactggct ttttttctgt attccaaaga tgcacgatgg
     8821 gacggaatgc cgctggcata tctttatgtg gctgcgatta ttccgacagc attgcttttg
     8881 ctgagacagg cgctggattt tacatacaga gaagaacttc tgatgaattc tttttctgat
     8941 tatgtggaac atgcccgcac atgggtagaa gaagtgccgg tgccggagaa ggctgacgaa
     9001 gctgtaaaag aggaaatcgt tgaacatatt gcggacagta tccgccagac tgcagcagca
     9061 ggaagccatt tcagcggaat gcttacaccc gaggaagatg agattatgag agaaatcata
     9121 agagaattta tgaaataaag acatgaaaat tcactgcaaa tgagatacac aggatcagaa
     9181 acaatatggt aatgtaaagt ttctgatcct gtacacattt taagacagaa taatttatta
     9241 ttcgtcatcc tcttcaatta cctggatctc cagataatca aaatcaatct gctctataaa
     9301 attatcctga taaaatctgg cttttccatg tttcttaaaa tgtctgactg tagattccag
     9361 ccagattggg tttcccagat ccatttcata acagatactt tccagaccac gaaaaatctt
     9421 gtgggtccgc gtatcttcgg agtcatcgca gattacagta tctttcagta aatgattatc
     9481 tttccatatt tttccccata aacggaacat atcggtaata cctccctgtc agaactgcca
     9541 caggcggcag aaatctcatt ttctaagtga ttcccagtat agcggatttt tgcgggaaag
     9601 taaagaaagt aaaaaaatgc tactgcttgt agtaactaaa atacttatag tcttaggctt
     9661 gattattccg gtggaatatt ctataataaa acctaaattt ccataaatta gcgtgtcaaa
     9721 gtgccggttc aggagaaaat cagttttcat ctggaaattt gaagactgat gtggataagg
     9781 tgagttccat aactgcattt gtgatatgca aaaaaacaga atggaggaga cattatgaaa
     9841 ttatacgata ccggtgtgta tttgctgaat ggtcagaaga ttgttccgga gaatcaggca
     9901 gattttcctg tttccaaaga ggaagctgca aagagtacaa ttgcctattc aattctgaaa
     9961 gcacacaata cttccggaaa tatggagaaa cttcagatta aatttgataa actgacttct
    10021 catgatatca cttttgtcgg aattatccag actgcacgtg cttcaggcct tgagaaattt
    10081 ccggtgccat atgtactgac aaactgccat aattcactct gtgccgtagg cggaactatt
    10141 aatgaagatg atcatatgtt tggacttacc tgtgccaaga aatatggcgg cgtatatgta
    10201 ccgccccatc aggcggttat ccatcagttt gcaagagaaa tgcttgcagc aggcggtaaa
    10261 atgatcctcg gttcagacag ccatacacga tatggcgcac tgggaaccat ggcaatgggt
    10321 gagggcggac cggagcttgt aaagcagctt ctttcccaga cttatgatat caatatgcca
    10381 ggcgtcgttg ccatttatct taccggttct cctcgtccgg gcgtaggtcc tcaggacgtt
    10441 gcgcttgcga ttatcggcaa ggtttttgca aacggatatg tgaagaataa agttatggaa
    10501 tttgtaggac ccggtgttgc aaatttaagt gctgatttcc gaattggtgt agatgtaatg
    10561 acaacagaga ctacctgtct ttcctctatc tggcagacag atgagaagat tcaggaattc
    10621 tatgagatcc atggcagaag tgaagattac aaagaactga atcctggtgc agttacatat
    10681 tatgatggta tggtctatgt gaacttaagc gagatacgtc cgatgatcgc aatgccgttc
    10741 catccgtcca atgtatatac aatcgacgac gtacgtaaga atctgaaaga cgtgcttcac
    10801 gatgtagagc agaaagcact ggtgagcctg gatggtgctg tggattattc acttcaggat
    10861 aaagtgattg atggaaaact ttatgtggat cagggaatca tcgcaggatg tgccggtggt
    10921 ggatttgaaa atatctgcgc agcagcagac atcataaaag gtcattacat tggctcagat
    10981 gaatttacat ttagtgtata tccggcaagt acaccgatct atatggaact tgtgaaaaac
    11041 ggtgcagttg cggatcttat ggaagcagga acaattgtaa aaacagcatt ctgtggacca
    11101 tgctttggag ctggagatac accggcaaat aacgcatttt ctattcgcca ctctacaaga
    11161 aacttcccga accgtgaagg ttccaagctt cagagtgggc agattgcatc cgttgcactt
    11221 atggatgccc gttcaattgc agcaacagca gcaaataaag gtttcctgac acctgcaaca
    11281 gatatggacg tagaatacaa gggacagaaa taccactttg accagaagat ttatgcaaat
    11341 cgtgtatttg acagtcatgg tgtggctgat ccggatacaa agatcaagtt cggaccgaat
    11401 atcaaagact ggccggcaat gtcagcactt ccagagaatc tggtattgaa agttgtatca
    11461 gagattcacg atccggttac aacaacagat gagctgatcc catctggaga aacatcttct
    11521 tacagatcca atccacttgg actggcagag tttgcacttt ccagaaaaga tcctgcatat
    11581 gtaggacgtg caaaggaagt acagaaagca cagaaagcaa tcgaagcagg cgaatgtccg
    11641 ttagaggtat tggaagaatt aaaaccggtt atggcaaaga tccaggagaa atatccggaa
    11701 gcaggaaaag gaaatatcgg agtcggaagt acgatctttg cagtaaaacc aggtgacggt
    11761 tcagcaagag agcaggcagc ttcctgtcag aaggtgctcg gtggctgggc gaatattgca
    11821 aatgaatatg cgacaaaaag atatcgctcc aa
//
LOCUS       DBMDXG010000013        11705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1563_length_11705_cov_1.948380, whole genome shotgun sequence.
ACCESSION   DBMDXG010000013 DBMDXG010000000
VERSION     DBMDXG010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1563_length_11705_cov_1.948380"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(130..996)
                     /locus_tag="ACZPD3_00940"
     CDS             complement(130..996)
                     /locus_tag="ACZPD3_00940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1414617.1"
                     /translation="MWIADGWKDYEVIDTSCGEKLERWGDYLLVRPDPQVIWDTPKVN
                     KGWKHMNGHYHRSKKGGGEWEFFDLPQQWDIHYKGLTFNLKPFSFKHTGLFPEQAVNW
                     DWFGNKIRNAGRPVKVLNLFAYTGGATLAAAAAGASVTHVDASKGMVTWAKENAVASG
                     LGDAPIRWLVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEDCIHDLIKL
                     CTKILSDEPLFFLINSYTTGLAPAVLTYMLSTELKKFGGHVDSQEIGLPVSSNGLVLP
                     CGASGRWEVINS"
     gene            complement(1675..2697)
                     /locus_tag="ACZPD3_00945"
     CDS             complement(1675..2697)
                     /locus_tag="ACZPD3_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1414618.1"
                     /translation="MKANVQKKKITGNGFLLLITIALFVVMYVAGMIVFADKGFAKPQ
                     MFLNLFVSNAGLLVIACGLTIVMITGGIDISVGSVTALVCMVLADLMENKGVNAYAAV
                     LVALLIGVAFGIVQGFLVAYLDIQPFIVTLAGMFFGRGLTAIISTDMISIKNELFLKW
                     ANYRFYMPFGSTSKKGKFIPAYIPPTVLIALIVVILIAVLLKYRKFGRKLYAIGGSRQ
                     SALMMGLNVKKTIFNAYVLDGFLAGLGGFLFCLNSCAGFVEQAKGLEMDAISSAVIGG
                     TLLSGGVGTPIGTLFGVLIKGTISSLITTQGTLSSWWTRIILSALLCFFIVIQSVVAS
                     MKKKAK"
     gene            complement(2697..3752)
                     /locus_tag="ACZPD3_00950"
     CDS             complement(2697..3752)
                     /locus_tag="ACZPD3_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1414619.1"
                     /translation="MNKSKLKKITSARLFLPIVCLIAVLLLNVIRTPDFFNVSIRNGV
                     LYGYIVDVINRASELVILAIGMTLVTAASGGQDISVGAIMAVAAAVCCQILSGGQVSV
                     NDYQNPVILAVIAALLASALCGAFNGFLVSKLNIQPMVATLILYTAGRGIAQLITNGQ
                     ITYIRVDSFKMAGGYIGKCPIPTPIFFAIITVVIVYLILKKTALGLYIESVGINGKAA
                     RLVGLNSTMIKFLTYVICGVLAGIAGIVASSRIYSADANNIGLNLEMDAILAVALGGN
                     FLGGGKFSLIGSVIGAYTIQALTTTLYAMNVKADQLPVYKAIVVVIIVTLQSDVFKKY
                     IANLKAKRSVAAEGGQK"
     gene            complement(3752..5272)
                     /locus_tag="ACZPD3_00955"
     CDS             complement(3752..5272)
                     /locus_tag="ACZPD3_00955"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1414620.1"
                     /translation="MEQNVVLEMRDISKNFTGVRALSHVDFTLRKGEIHALMGENGAG
                     KSTLIKVLTGVHEFESGTIHMAGNSNAIINHSPQEAQANGISTVYQEVNLCPNLTVAE
                     NLFIGREPRKMGMIDWKQMNERSGKLLESLDIHVPPTQMLDECSIAIQQMIAIARAVD
                     MKCQVLILDEPTSSLDDDEVEKLFVLMRRLRDEGVGIIFVTHFLEQVYAVCDRITVLR
                     NGELVGEYETKDLPRVMLVAKMMGKDFDDLADIKGDHKDKRITDAEPVIEAKGLSHKG
                     TIKPFDITINKGEVIGLTGLLGSGRSELVRAIYGADKADTGTLKVKGKEVKINTPLDA
                     MKLGMAYLPEDRKAEGIIADLSVRENIIIALQAKRGMFHPLSKKEMEDAADKYIKLLQ
                     IKTASRETPIKSLSGGNQQKVILGRWLLTNPDYLILDEPTRGIDIGTKTEIQKLVLDL
                     ADQGMAVTFISSEVEEMLRTCSRMAVLRDGQKVGELEEDELSQSGVMKAIAGGDDK"
     gene            complement(5462..6520)
                     /locus_tag="ACZPD3_00960"
     CDS             complement(5462..6520)
                     /locus_tag="ACZPD3_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1414621.1"
                     /translation="MKKKIVSALLTATMVCGMSVVPAVGVAAADDTITVGFSQVGAES
                     DWRTANTESMKSTFSEENGYELIFDDAQQKQENQLTAIRNFIQQEVDYILLAPVTETG
                     WDTVLQEAKDADIPVIIVDRMVDVSDDSLYTTWIGTDSLLEGRKAAEWLNAYTTAKGI
                     DAKDVNIVDIQGTIGSTAQIGRSKGLEEGVDNYGWNLLAQQSGEFTQAKGQEVMESML
                     KQYDNINVVYCENDNEAFGAIDAIEAAGKTVGSDIANGEIMVMSFDTTNAGLTDTLAG
                     KIACDVECNPLHGPRAEELIKALEAGEEVEKLNYVDEEIFATDDTVDKVKAVNSLDEE
                     GEYDVTPITQEIIDGRAY"
     gene            complement(6924..8375)
                     /locus_tag="ACZPD3_00965"
     CDS             complement(6924..8375)
                     /locus_tag="ACZPD3_00965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414622.1"
                     /translation="MKQKQEKLETKMKWMLLSTMIPMTILIIVLLVFFWHYTNEYNQL
                     SNNLAVSSEYNANYRNSNNDMSFKDEVDMDIYYVTIGRKGKDGLPTEQVDKAISTVES
                     LKKTTSKKESLRSLKYLTNYLENLKKRMNQLLEIKNYQERQEFVANNTEILTTLFEKE
                     MQNYIYQEATELVQIESQLAGNVRLTIITMCAAILVATAILLRRSFRLTYSVTKPISE
                     ILHNIKKVGKGEYKTINVVSADSVEIQELDAGTRKMAGRIEGLLENVRKEQEVQHMTE
                     LQLIQAQVNPHFLYNTLDTIVWLVEGGMQQDAVDMITSLSVFFRTSLSKGKDIIPLSE
                     EKRHTLSYLEIQQSRYRDIMEFEINIPPELDEVMVPKLTLQPLAENALYHGIKNKRGK
                     GKILIEGFNLGDDMMLRVTDNGQGMTPERLYEVQEAIRTGERAGFGLAAVSERIALYY
                     GPGYGLKISSTEGEGTVMEVYMAKKINLDNNSR"
     gene            complement(8379..10025)
                     /locus_tag="ACZPD3_00970"
     CDS             complement(8379..10025)
                     /locus_tag="ACZPD3_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1414623.1"
                     /translation="MLKTFLVEDEVVIREMIKKMIPWEQYGFELAGEASDGEMALPLI
                     LKSKPDLLITDIKMPFMDGLTLCKLVKKELPDIKIVILSGYDDFNYAKQAINIGVEDY
                     LLKPITKNAFIERLEEIHNRYEHEKTQKEYYEKFKLEMQEYERNASRDFFESLVRADF
                     DLEEIYRRADRLNLDIVAEAYNILIFTPDASDSSCNSSEGYSDWEAEVHKKIENYFLS
                     HPVAMLFRHQVFSYAILVKGQRDTIKKNTCECVETIQKIMEETRANVDWFVAVGEEAD
                     RLSRIKQSYHTAARTYAFRYLYDGHILYYNMLEQVKENSADTSKTEAVQLKNVNINAL
                     NPEILQKFLSSGLEDEVDSFVHDYFHAIGREPMKSLVFRNYVVLNVRFSVLSFLKKIG
                     YDDTELSREETDDVVKKTSQSTEASVAYAEEVLKRAIAIRDENAGSQNRSVLKQAIDF
                     IDGHYMDEEISLNRVAHAANVSANHFSALFSQNMGQTFIEYLTSLRMDKAKELLRCTS
                     KRSSEIAGEVGYRDAHYFSYLFKKTQGMTPSEYRKTRGEV"
     gene            complement(10535..>11705)
                     /locus_tag="ACZPD3_00975"
     CDS             complement(10535..>11705)
                     /locus_tag="ACZPD3_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR1414624.1"
                     /translation="KDSNWYKLILRIYELDPGVREAFFTNFIINASLKGSALQEETAE
                     ENNCNVPWAILLDPTSACNLHCTGCWAAEYGHKLNLDFDTICSIVEQGRKMGTYMYIY
                     TGGEPLVRKKDLMRICEKYPDCEFLSFTNGTLIDEEFCQEMLRVKNFVPAISLEGSEE
                     ANDGRRGEGVYQKVMHAMELLKAHKLPFGVSTCYTSANVDSVSSEEFFDHIIDCGALF
                     VWFFHYMPTGNDAVVELMPNPQQREKMYHKIREYRSTKAIFGMDFQNDAQYVGGCIAG
                     GRRYLHINANGDVDPCVFIHYSNANIYENTLLEALKSPIFMAYHDGQPFNENMLRPCP
                     MLENPQKLRKMVEESGAKSTDLQSPESVEHLCAKCDAYAEHWAPKAEELFPVKNK"
BASE COUNT         3391 a         2945 c         1937 g         3432 t
ORIGIN      
        1 tacgcctata agttttgaaa attcatgtat agaataatat ttactcaaag catcaactcc
       61 tttcaataat attctactct aatgtcttat aaaactcaac atatgcttat ataattttat
      121 aatttatatt taactgttaa taacctccca tcttccagaa gcaccacatg gaagaaccag
      181 tccgttagag cttaccggaa gcccgatctc ctgagagtcc acatgcccgc cgaacttttt
      241 caattcagta gacaacatat aagtaagaac tgccggtgcc agacctgttg tataagaatt
      301 tatcaggaaa aacaaaggct catcgctgag aatctttgta cacagcttaa tcaggtcatg
      361 gatacaatcc tcaatcttcc agatctcccc cttcggtcca cgtccataag aaggcggatc
      421 cataatgatc gcatcataat gattccctct gcgaatctca cgctcaacaa acttcacaca
      481 gtcatccacc agccagcgga tcggagcatc tcccagaccg gaagcaaccg cattctcctt
      541 tgcccaggta accataccct tagaagcatc cacatgagta acagaagctc ccgcagccgc
      601 tgccgccaga gtcgcaccac ctgtatatgc aaaaagattc aacaccttca ccggacgtcc
      661 cgcattgcgg atcttattgc caaaccagtc ccaattcaca gcctgctccg gaaaaagccc
      721 ggtatgctta aaactaaaag gtttcaaatt aaaggtaagt cccttataat gaatatccca
      781 ctgctggggc aaatcaaaaa actcccattc accgccgccc ttcttacttc tgtgataatg
      841 cccgttcata tgcttccagc ccttatttac cttcggcgta tcccatataa cctgcggatc
      901 cggcctcacc agaagataat ccccccaacg ctccagcttc tccccacagg aagtatcaat
      961 aacctcataa tctttccatc catctgcaat ccacattgta taataatcct cctaaaataa
     1021 acgcataata aattacgatt gtactacata tccaaccatt agtaaagcca catttctcac
     1081 ataatttcaa taatatcaag atcaaaatcc cctttaacct taacatcatt cattgtttaa
     1141 agtataaaat aaaactcccc ccatggcaag caaaccatca taattcacag ttataaacaa
     1201 ataaagcatt actgttcgct ccgttctcaa taacacgctt cgcaggatat tactagcgaa
     1261 caataataat aaagtatcga tactcacgtt caagttaata acatcatcct cttcgcaacc
     1321 tgcccatacc ccaatatctt ctccacagtt tatcagaaat ttacttgctt cgctctaaat
     1381 cgttgccagt acccagcgcg aaacagcatc tctctgtggg tgaatctgga gggggcttag
     1441 taactcttag gcggagaaaa tccgcgttat gaagaaaaac agcaacctgt ctgagcgaag
     1501 tcggaggcgg agcgagtttt gcgtttttct tcatgctttt agcggatttt ctccacctta
     1561 gagttactta gcccccggaa ctgaacccac agagagatgc tgtttcgcgc gttcctctac
     1621 aaaaaaacaa gccaagactc ccaaaagtct cagcttgttt cataatacaa ccacttattt
     1681 cgctttcttc ttcatagaag ccacaacaga ctgaataaca ataaagaaac aaagcaacgc
     1741 agaaagaata attcttgtcc accagcttga caaagtaccc tgagtagtaa tcaaactgga
     1801 aatcgtaccc ttaatcaaca caccaaacaa agtaccaatc ggagtaccaa caccacctga
     1861 aagaagcgta ccaccaataa ccgctgatga aattgcatcc atctcaagac cctttgcctg
     1921 ctcaacgaaa cctgcacaac tgttcaaaca gaacaggaag ccaccaaggc ctgcaagaaa
     1981 accatccaga acataagcat taaaaatagt tttcttaaca ttcagaccca tcattaatgc
     2041 actctgacgg ctaccgccga tcgcataaag cttacgtcca aacttacgat atttcagaag
     2101 aaccgcgatc agaattacaa caatcagagc aatcaaaact gtcggcggaa tataagccgg
     2161 aataaattta cccttcttac tggttgaacc aaaaggcata tagaaacggt aatttgccca
     2221 tttcaggaat aactcattct tgatagaaat catatcagta ctgatgatag ctgtcagacc
     2281 acgtccgaag aacatacctg ccagagtaac gatgaatggt tggatatcca gataagctac
     2341 caggaatccc tgaacaatac caaaagcaac accaataagc agtgcaacta aaactgctgc
     2401 atatgcgttc acacctttgt tctccataag gtctgcaaga accatacata caagtgcagt
     2461 aacagaacct acggaaatat caataccacc ggtgatcata acgattgtaa gaccacacgc
     2521 aataaccaga agacccgcat tagatacaaa caggttcaga aacatctgag gttttgcaaa
     2581 acctttatca gcaaaaacaa tcatacctgc aacatacatt actacaaata atgcgattgt
     2641 gatcagaagc aggaagccat ttcccgttat tttctttttc tgtacatttg ctttcattac
     2701 ttctgacctc cttctgctgc cacacttctt ttagccttta agttggcaat atatttctta
     2761 aatacatcac tctgcagtgt aacgatgata acaactacga ttgccttata aacaggaagc
     2821 tggtctgctt ttacgttcat cgcatagagt gttgtggtca gagcctgaat agtgtaggca
     2881 ccgattacgg aaccgatcag gctgaattta ccaccaccaa ggaagtttcc gccaagcgca
     2941 actgcaagga tggcatccat ttccaggtta agaccaatgt tgtttgcatc tgcagagtag
     3001 atacggctgg atgccacaat accggcaata ccagccagta caccacagat aacataggta
     3061 aggaatttaa tcatcgtaga atttaatccc acaagacgag ctgcctttcc attgatacca
     3121 acactttcaa tgtacagccc aagagcagtc ttcttcaaaa tcaggtacac aataacaact
     3181 gtaatgatcg caaagaaaat aggtgtagga atcgggcatt ttccaatata tccaccagcc
     3241 atcttaaagg aatccacacg aatataagta atctgtccgt ttgtgatcag ctgagcaata
     3301 ccacgaccgg ctgtgtaaag gatcagagtt gcaaccattg gctgaatatt cagcttggaa
     3361 accagaaatc cattaaatgc accacataat gcagatgcaa gaagcgctgc gataactgcc
     3421 aggatcacag gattctggta atcatttaca gaaacctgtc cgccggaaag aatctgacag
     3481 cagacagccg ctgcaactgc catgatcgca ccaacactga tatcttgtcc accggaagct
     3541 gctgttacca gtgtcatacc gattgcaagg ataaccagct ctgaagcacg gttaataaca
     3601 tcaacaatgt aaccataaag tacaccgttt cggattgata cattaaagaa atccggtgtt
     3661 ctgattacat tcagcaaaag tactgcgatc agacacacaa taggaagaaa cagacgtgca
     3721 cttgttattt tctttaattt actcttattc attatttatc gtcacctccg gcaatagctt
     3781 tcataactcc gctctgggaa agttcatcct cttccagctc ccctaccttc tggccatcac
     3841 gaagaactgc catacgggag caggtgcgga gcatttcctc aacctctgag gagataaatg
     3901 taactgccat tccctgatcc gcaagatcaa gaaccagttt ctgaatctct gtcttagtac
     3961 cgatatcaat acctctggtc ggctcatcca gaatcagata atccggatta gtcagaagcc
     4021 atcttccaag gataaccttc tgctggttac caccggacag actcttaatc ggagtttcac
     4081 gacttgctgt cttaatctgc agtaatttaa tatacttatc agcagcatcc tccatttctt
     4141 tcttgcttaa tggatggaac ataccgcgtt tagcctggag cgctataata atattttctc
     4201 ttacagaaag atctgcaata ataccttctg cctttctgtc ttccggaaga tatgccatac
     4261 caagtttcat ggcatccagc ggagtattga tctttacttc ttttcccttt acttttaaag
     4321 ttcctgtatc agctttatcc gccccataaa ttgcacgaac caattcagaa cgtccggaac
     4381 ccagaagtcc ggtaagacca ataacctcac ctttattgat ggtaatatca aacggtttga
     4441 tcgttccctt atgagataat ccttttgcct cgattacagg ttctgcatct gtgattctct
     4501 tgtctttatg atcgccttta atatctgcaa gatcatcaaa atccttaccc atcatcttgg
     4561 caaccagcat tactctcgga agatctttcg tctcatattc cccaacaagt tcaccattac
     4621 gaagaactgt aatcctgtcg cagactgcat atacctgctc aaggaaatgc gtaacaaaga
     4681 taatacctac accttcgtca cgaagtcttc tcataagtac aaacagcttt tcaacttcat
     4741 catcatccag tgaagatgtc ggttcatcga gaatcagtac ctggcacttc atgtccactg
     4801 cacgggcaat tgcgatcatc tgctgaatcg caatggaaca ttcatccagc atctgtgttg
     4861 gcggcacatg aatgtccagg ctctcaagaa gttttcctga gcgctcattc atctgcttcc
     4921 aatcaatcat tcccatcttt ctcggttctc tgccaataaa aaggttctca gctacagtca
     4981 ggttgggaca aaggtttacc tcctgatata ctgtactgat accatttgcc tgcgcttcct
     5041 gaggcgagtg gttaataatg gcattactgt ttccagccat atgaattgtt ccactttcaa
     5101 attcatgtac accggtcagc accttaatca gtgttgattt acctgcacca ttctccccca
     5161 tcagtgcgtg gatttcgccc tttcgaaggg taaagtccac atgggaaagg gcacggactc
     5221 ctgtaaagtt tttgctgata tcacgcattt ccaatactac attttgttcc atattctttc
     5281 cacctgcttt ctatactggt ttacccctca tttactgaaa agcttcagca aagcgggtat
     5341 ctgtaaatca tttcggcata aagagagcgt gattcataaa tccgggtttc tccgaattat
     5401 gaaccacgct ccggattatt tatattcctt ctattacact tctattattt gttgtcaaaa
     5461 attagtatgc acgtccgtca ataatttcct gtgtgatagg tgttacgtca tattcgccct
     5521 cttcatcaag actgtttaca gctttaactt tatcaactgt atcatctgta gcaaagattt
     5581 cttcatctac atagttcagt ttttcaactt cttcaccagc ttcaagagct ttgatcagtt
     5641 cttctgcacg tggtccgtgt aatgggttac actcaacatc gcatgcgatc ttgccggcta
     5701 atgtatctgt taaaccagca tttgttgtat caaaagacat aaccatgatt tcaccgtttg
     5761 cgatatcaga tcctactgtt ttaccagctg cttcgatagc atcgatagca ccgaatgctt
     5821 cgttatcgtt ctcgcagtat acaacattaa tattgtcata ctgtttcagc atggactcca
     5881 taacttcctg tcctttagcc tgtgtgaatt caccggactg ctgagcaagt aagttccatc
     5941 catagttgtc aacaccttct tcaagacctt tggaacgtcc aatctgagct gtagatccaa
     6001 ttgtaccctg gatatcaacg atgttaacat cttttgcatc gattccttta gctgttgtgt
     6061 atgcattcag ccattcagct gcttttcttc cttcgagtaa ggagtcagtt ccgatccatg
     6121 ttgtatataa gctgtcatca gatacatcaa ccatacggtc tacgatgatt acaggaatat
     6181 cagcatcttt agcttcctga agaactgtat cccaacctgt ctctgttaca ggagctaaaa
     6241 gaatgtaatc aacttcctgc tggatgaagt tacggattgc tgttaactgg ttttcctgtt
     6301 tctgctgtgc atcgtcgaag attaattcat atccgttctc ttcgctgaat gtggatttca
     6361 tggattctgt attagctgta cgccagtcag attcagcacc tacctgtgag aaaccaactg
     6421 tgattgtgtc gtccgctgct gctacaccca ctgccggaac aacgctcata ccgcatacca
     6481 ttgttgctgt taataaagct gacacgattt ttttcttcat tttgtaaatc ctcccgattc
     6541 atttaagatg tctttattat agtaaaaatc caggaaaatt ccatagaata ttttccgcaa
     6601 aaagtttatt cttctatcat attttgttca taatttatac aaagtttatt tttttattgc
     6661 ggaagttgga ttttttattg tgcattataa atacagaaat aaattatcaa ctttctttta
     6721 tattcacttt gcacaattca gcattagtcg acataattca tacattccat tatttggcag
     6781 accttcgtaa gtctctatca gcaggccata aaactgttat tggatatgaa taaacaataa
     6841 aataattgac tttatccacc tgaatacata acaaaagctg catatctgtt aagattctta
     6901 acttcatgca gctttttctt tgtttatctt gaattgttat ccaggttaat ttttttcgcc
     6961 atatacactt ccataaccgt accttctccc tctgtagaag aaatcttcag tccataaccc
     7021 ggaccataat aaagtgcaat tctctctgac accgcagcaa gtccgaaccc ggcacgttca
     7081 ccggtgcgaa tggcttcctg tacttcataa agacgttccg gtgtcattcc ctgaccattg
     7141 tcagtcactc tcagcatcat atcatctccc agattgaatc cttcaataag aatcttacct
     7201 tttccacgct tattcttaat tccatgataa agagcattct ccgctaacgg ctgcaaagta
     7261 agcttcggca ccatcacttc atccagttcc ggcggaatat taatctcaaa ttccataata
     7321 tccctgtatc ttgactgctg aatctccaga taactcaatg tgtggcgttt ctcttccgac
     7381 agcggaatga tatccttccc cttcgaaagc gaagtgcgga aaaaaacaga cagacttgtg
     7441 atcatatcca ctgcatcctg ctgcatacca ccctccacca gccacacaat agtgtccagt
     7501 gtattataca gaaaatgcgg atttacctgt gcctggatca gctgcagctc cgtcatatgc
     7561 tgtacctcct gctccttacg cacattctca agaagccctt caatccttcc tgccatcttt
     7621 ctggtaccgg catcaagctc ctggatttca acactgtcgg cagatactac attaatagtt
     7681 ttgtactctc cttttccaac cttcttaata ttatggagaa tttcagaaat cggtttggta
     7741 acgctgtatg tcagacggaa agaacgccgc agcagaatag ccgtagcaac caaaatcgcc
     7801 gcacacatag tgatgatcgt cagacgtaca tttccagcca gttgagactc tatctgcacc
     7861 agttccgtcg cctcctgata aatataattc tgcatttctt tctcaaagag cgtggtcagg
     7921 atttctgtat tatttgccac aaattcctgt cgttcctgat aattctttat ctcaagaagc
     7981 tgattcatcc gtttcttaag attctccaga tagttagtca gatatttcag agaacgcagg
     8041 ctttccttct ttgatgtggt tttcttcaga ctctccacag tactgattgc cttgtctacc
     8101 tgctctgtcg gcagcccgtc cttacctttt cgcccgatag tcacataata gatatccata
     8161 tccacttcat ctttaaagct catatcatta ttggaatttc tatagtttgc attatattcc
     8221 gaactcaccg ccagattatt agacaactga ttatattcat ttgtataatg ccagaaaaaa
     8281 acaagaagca ctatgatcag aattgtcata gggatcatag tgctcagaag catccacttc
     8341 atctttgttt ccagcttctc ctgtttctgc ttcatgaatc agacttctcc tctcgtctta
     8401 cgatactcac tcggagtcat tccctgagtt ttcttaaaaa gatagctgaa gtaatgcgca
     8461 tccctatatc ccacttcccc tgcaatctca ctggaacgtt tggaagtaca gcgaagcagc
     8521 tcctttgcct tgtccatgcg aagcgaagtc agatactcaa taaatgtctg tcccatattc
     8581 tgactgaaaa gcgcactgaa atgattggca ctcacattcg ccgcatgcgc cactctattc
     8641 agagaaatct cttcatccat ataatgtcca tctataaaat ctattgcctg cttcaacaca
     8701 cttctgttct gactgcctgc attttcatca cggattgcaa tggcccgttt caagacttcc
     8761 tctgcatatg caacagaagc ttctgtagac tggcttgtct tcttcacaac atcatctgtc
     8821 tcttctctgg acaattccgt atcatcatat ccaattttct taagaaatga cagtacagaa
     8881 aaacgcacat tcaaaaccac ataattacgg aacaccagtg atttcatcgg ttccctgcca
     8941 atagcatgga aataatcatg gacaaaactg tctacttcat cttccagacc actgcttaaa
     9001 aatttctgaa ggatttccgg attcagggca ttgatattca cattcttaag ctgaacagct
     9061 tctgtttttg aagtgtctgc tgaattctcc tttacctgct caagcatatt gtaataaaga
     9121 atatgcccgt catacagata acggaatgca taagttcttg cagccgtgtg ataactctgc
     9181 ttaattctgc tcagacggtc tgcctcctcg ccgactgcca caaaccagtc cacatttgct
     9241 ctggtttctt ccatgatctt ctgaattgtt tccacacatt cgcatgtatt cttctttatc
     9301 gtatctcgct gccccttgac cagaatcgca taactgaaca cctgatgtct gaaaagcatc
     9361 gcaaccggat gactgaggaa ataattctca atcttcttat gtacttctgc ctcccagtct
     9421 gaatatcctt ccgacgaatt acaactgctg tctgaagcat ccggtgtaaa aatcagaata
     9481 ttataagcct ctgccacaat atcaagattc aaacgatctg ctctccgata aatctcttcc
     9541 agatcaaagt ccgctctcac cagagattca aagaaatcac ggctcgcatt cctctcatac
     9601 tcctgcatct ccagtttgaa tttctcataa tattccttct gcgtcttctc atgttcataa
     9661 cgattatgaa tctcctccag acgctcaata aatgcattct tagtaattgg cttcagcaga
     9721 taatcctcca caccaatatt gatcgcctgc ttcgcatagt taaagtcatc atagccacta
     9781 agaatcacaa tcttaatgtc cggcaactcc ttctttacca atttacataa tgtcagacca
     9841 tccataaacg gcatcttaat atcagtgatc agaagatccg gtttgctctt taatatcaac
     9901 ggcagtgcca tctccccatc agatgcttct cctgccagtt caaacccata ctgctcccag
     9961 ggaatcatct tcttaatcat ctctctgatc acaacttcat cctctaccaa aaaagttttt
    10021 aacatcccta ccactctctc atactaaaaa atctacagca atcctcttaa atacattatt
    10081 tacgagaact tctacatatc catcatacag gttaacacca taactttaat acatgtcgca
    10141 cactcaggca agttattttt aacaataaaa tgcttcacac acttccttcc acaatcaatc
    10201 aacagcgcga aacagcatct ctctgtgggt gaatctggag ggggcttagt aactcttagg
    10261 cggagaaaat ccgcgttatg aaaaaaagca gcaacctgtc tgagcgaagt ttgcaacgga
    10321 gcgagttttg cgtttttctt catgctttta gcggattttc tccaccttaa agttacttag
    10381 cccccggaac tgaacccaca gagagatgct gtttcgcgcg tccccttatt caaagacgag
    10441 aaagttcccc atccggacag gggatgaccg gacagggaac tttctctgat aactttggct
    10501 aaattttgag ttaatatttt ttcttattat taagttactt attttttaca ggaaataatt
    10561 cctcagcttt cggtgcccaa tgctctgcat aagcatcaca cttcgcacag agatgctcca
    10621 cagactccgg tgattgcaaa tctgtagact tcgcaccgga ttcttcaacc atcttacgaa
    10681 gcttctgcgg attctcaagc atcgggcatg gacgaagcat attttcatta aacggctgac
    10741 cgtcatgata tgccatgaaa atcggactct tcaatgcttc cagcagagta ttttcataaa
    10801 tatttgcatt agaataatgg ataaatacac atgggtctac atcaccattc gcattgatat
    10861 gaaggtaacg tcttcctcct gcaatacatc ctcctacata ctgcgcatca ttctggaaat
    10921 ccattccaaa aatagctttt gtagaacgat actctctgat cttgtgatac atcttctcac
    10981 gctgctgtgg attcggcatt aattctacta cagcgtcatt acctgtcggc atataatgga
    11041 aaaaccatac aaataatgca ccacagtcaa taatgtgatc aaaaaattct tcgctgctta
    11101 cactgtccac atttgcagaa gtataacagg tagatacacc aaacggaagt ttatgagctt
    11161 tcagaagttc cattgcatgc ataactttct gatatacacc ttctccacga cgtccgtcgt
    11221 ttgcttcctc agaaccttcc aggctgatcg ccggaacaaa attcttaaca cgaagcattt
    11281 cctggcagaa ctcttcgtcg atcagtgtac catttgtaaa ggaaaggaac tcgcagtccg
    11341 gatatttctc acagatacgc atcaggtcct tcttgcggac cagcggttca ccacctgtat
    11401 atatgtacat ataagttccc atttttctgc cctgctctac aatagaacag atggtatcaa
    11461 aatcaagatt cagtttgtga ccatactctg ctgcccagca tcctgtacag tgcagattac
    11521 aggcacttgt aggatcaaga aggattgccc atggaacatt gcagttgttc tcttctgctg
    11581 tttcttcctg aagtgcactt ccctttaagc tggcattgat aataaagttt gtaaagaatg
    11641 cttcacgtac tcctggatcc agctcataaa tcctcaggat cagtttatac cagttgctgt
    11701 cctta
//
LOCUS       DBMDXG010000014        11478 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1599_length_11478_cov_2.429463, whole genome shotgun sequence.
ACCESSION   DBMDXG010000014 DBMDXG010000000
VERSION     DBMDXG010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1599_length_11478_cov_2.429463"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(537..812)
                     /locus_tag="ACZPD3_00980"
     CDS             complement(537..812)
                     /locus_tag="ACZPD3_00980"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR1414625.1"
                     /translation="MRENLIRKHYWFSGRVQGVGFRYRACYIASSLGVTGWVRNNWDD
                     RVEMEAQGSRELLAQMVEMLGRQRFIEIEGIEERVIPVEVESGFYSR"
     gene            complement(1060..1578)
                     /locus_tag="ACZPD3_00985"
     CDS             complement(1060..1578)
                     /locus_tag="ACZPD3_00985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR1414626.1"
                     /translation="MNETIKNLLERRSVRGYKEDLVPEEVLNEILEAGEYAPSGMGQQ
                     GTLMVVTQNPELVAKLSKMNADVMGTESDPFYGAPTVVVVFADSNMPTCVENGSLVMG
                     NLMNAAHALGVDSCWIHRAREVFASEEGKALKAEWGVPESYVGIGHCVLGYRSGEYPK
                     AKARKDGFVIRV"
     gene            1797..2993
                     /locus_tag="ACZPD3_00990"
     CDS             1797..2993
                     /locus_tag="ACZPD3_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 domain-containing protein"
                     /protein_id="HIR1414627.1"
                     /translation="MNKKEISEIKKQFTPENCSLTRICGCYVDGEKNKKTELKEAFLS
                     LSEEEMFKYFEIFRKSLSGTIGKNLMNMEFPLEQETEGGTQHFLMKLRESKLTDDALV
                     ESFYDKIIENYDYPENYYIILIHGVYDIPGKSSDGAEMFDASDEIYEHIMCCICPVKL
                     SKAGLCYNAETNSIQDRIRDWIVELPDVAFLFPQFNDRSTDIHGVLYYSKNANELRDI
                     FIDELLGCTAPLSAGGQRDSFNALVEDTLGDDCRYDTVLSIHEKLNDLIESQKDEPEP
                     VVLTKSEVKRLFEECGVEDEKLQSFDEQYEITAGEKSSLVASNITNTKRFEIKTPDVV
                     VHVDPERADLVETRVIDGRKCLVIPMEGEVELNGIRVHTGNENPSDDEFYDNTDTETE
                     ETSDGE"
     gene            2983..3783
                     /locus_tag="ACZPD3_00995"
     CDS             2983..3783
                     /locus_tag="ACZPD3_00995"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1414628.1"
                     /translation="MENNRKLLFFDIDKTLLTPYPWTVPDSAKQALKEARYNGHLLFI
                     NSGRTYTMIPEIIKEMGFDGYVCGCGSQIYMNEELLFSSSIPNPLCRKTTELLRECRI
                     PAFFERPDKILYDSNSCAVPETVQKLKAEAIVEDLALYPPETYNHFTFDKFLAFPSAD
                     SDMETFRKFADEHFLCFIHEDKAWELTQKNLSKATGIRFLADRLGVPVKDTYAFGDST
                     NDLLMLQFAGNSIAMGDSDPLILPHCTYQTTNIEDNGIANALKHFHLI"
     gene            complement(3986..5227)
                     /locus_tag="ACZPD3_01000"
     CDS             complement(3986..5227)
                     /locus_tag="ACZPD3_01000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1414629.1"
                     /translation="MKKMVKRTAVVTLAGVMSVGMLSGCGSKTLDGTKTVATVDGTDI
                     PLGVVSLYAREQQQQTTTMYLNYMGSADNIWDQTAGDDSDETYGDQAVTSSLESVEKM
                     YILKEKAADYNVELTDDDEAAIADAASQFMAANSEETIKELAVTEDQVKTLLELQTIQ
                     KKMYDPVVAEGKITVSDDEANQTTFTYVSISTSGDDITDEEKKTKKEQAQEILDKMKE
                     DPTADMSEIAKGVDDSYSAVQGNFTTKESKDEDKDSGSEAYPDEVLKVLRGLKDGEVA
                     DNIIETDTGYYVVRLDKINDEDATASKRESLQNSKESTYFTDTTAKWLDEADVKVVKK
                     VIKTLKITDKHTFMAPTPTPVPETPTPEVTEEATPTPETEEVTETPAAEDTETTETPA
                     AEEEAAETTETPEATPTEAAK"
     gene            complement(5350..8670)
                     /gene="mfd"
                     /locus_tag="ACZPD3_01005"
     CDS             complement(5350..8670)
                     /gene="mfd"
                     /locus_tag="ACZPD3_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR1414630.1"
                     /translation="MKAFLTPLQGLAEFEQIKEKSKTNKGILQVSGCMESQKSHLMYG
                     LSGIAPYRLILAEDERRAREIYEDYRFYDRKVYSYPAKDLLFFQADIHGNLLIRQRMK
                     VIKALLEEKELTVVTSIDGCMDFLESLEKIKEQLIHYESDSTVDTEQLKNQLVALGYE
                     RVGQVEMPGQFSVRGGIVDIYCLTEENPWRIELWGDEIDSIRSFDPESQRSLENLEEL
                     TIYPAVEHIGDKDMVSFLDYFPEERTIIFLDEPNRLTEKGGAVEEEYRQSRMHREEKG
                     SRNLPENWLCSFEQLQKELNKRNCISVCALEPKQAGWKVREKFYLEVKSISAYNNSFE
                     LLVKDLHQYKKQGYRIALLSGSRTRAERLAKDLQEEGLAAFYGQDYDREICPGEIMVV
                     YGHAKKGFEYPLIKFAVMTESDIFGQEQKKKKKKKNYSGSRIQDFAELSIGDFVVHEK
                     HGLGIYRGIEKVEVDRIVKDYIKIEYRGGSNLYIPATQLDCLQKYSGADASKAPKLNK
                     LGTQEWNKTKSKVRGAVKNIAKELVELYAVRQEKEGYVCGPDTVWQREFEEMFPYEET
                     EDQLSAIEDAKRDMESTRIMDRLICGDVGYGKTEVALRAAFKEVQESRQVAYLAPTTI
                     LAQQIYNTFVQRMKEFPVRVELLCRFRTPAQQKKAIEDLKKGQVDVIIGTHRILSKDV
                     QFKNLGLLIVDEEQRFGVTHKEKIKQLKKDVDVLTLTATPIPRTLHMSLIGIRDMSVL
                     EEPPMDRMPIQTYVMEYDEETVREAINRELRRGGQVYYVYNRVTDIADVALRIAKLVP
                     DARVDFAHGQMSERELENVMYSFVNGDIDVLVSTTIIETGLDISNVNTMIIHDSDRYG
                     LSQLYQLRGRIGRSNRTAYAFLMYRKNVMLKETAEKRLAAIREYTDLGSGFKIAMRDL
                     ELRGAGNLLGAQQHGHMNAVGYDLYCKMLNEAVKEAKGIHTMEDFETSVDLNVDAYIP
                     DSYISNEFQKLDIYKRIAGIETQQDYDDMLEELLDRFGEPGKAVLNLLAIAKLKAIAH
                     QGYVTEIKQTGKTVRFTLYEKARLNTEGFPALMQKYRRGLQFKNEQEPKFILEPQGNL
                     ILALTEFAEELKSMAENM"
     gene            complement(8770..9351)
                     /gene="pth"
                     /locus_tag="ACZPD3_01010"
     CDS             complement(8770..9351)
                     /gene="pth"
                     /locus_tag="ACZPD3_01010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR1414631.1"
                     /translation="MYLVVGLGNPGKQYEATRHNMGFDTVDRLVEDYNVPQGGVKFNA
                     MYGKTMIGGEKVILMKPLSFMNLSGGPVREMANYFKIDPESELIVIYDDIDLEPGQLR
                     IRKQGSAGGHNGIKDIIRQLGTEKFLRIKVGVGAKPKGWDLADHVLGRFSTEDRKLVD
                     EAITKAAKAVDIIISQGTDAAMNEYNRKVPVQK"
     gene            9439..9522
                     /locus_tag="ACZPD3_01015"
     CDS             9439..9522
                     /locus_tag="ACZPD3_01015"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1414632.1"
                     /translation="MCSKTNPANCNARLAESIFQTHAKIIN"
     gene            complement(9515..10465)
                     /locus_tag="ACZPD3_01020"
     CDS             complement(9515..10465)
                     /locus_tag="ACZPD3_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIR1414633.1"
                     /translation="MDDIRVPIYLVTGFLESGKTTFLDFTLQQEYFAIDGKTLLILCE
                     EGEEEYDMDKLKLTNTVVEVIEDEEDLTPQRLAAMDIIHQPERVVIEYNGMWLVSKFE
                     QMELPEGWGIEQEITCVDATTYQVYMANMKSLFMDMIRNTDMVVFNRCKEGDPLPAYR
                     RGIKVANQSAEVIFENEEGEMDDIFQDEMPFDINAPIIEIPPEDYGIWFVDAMDNPDK
                     YVDKIVRFKGRVMKPRGMGSKFFVPGRVAMTCCADDTTFLGYVCRSDYAPHIKEGSWV
                     EVTAKVAFENRTEYQGEGIVLYASDVKECEPLKEEMVYFN"
     gene            complement(10622..>11478)
                     /locus_tag="ACZPD3_01025"
     CDS             complement(10622..>11478)
                     /locus_tag="ACZPD3_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR1414634.1"
                     /translation="LSDVIKAVQGVQGDGDIVLNSYTTVVDAKKCKMYMRNFGEFFDN
                     QVEYAGAIIMSRTDIIDEKKAQQAMELLRGINAKAAIITTPIEKLDGKKILEVMEKPV
                     SLEEELMSEEEVCPECGHVHEHGEHHHHDHDHDHECGCGHDHEEHEHHHHDHDHECGC
                     GHDHEEHEHHHHDHDHECGCGHDHHDHHHHHADEVFTSWGRETIKKYTREGLEKMLEA
                     LSASEDYGIILRAKGMLPAEDGTWIYFDMVPEETEIREGAPEYTGRLCVIGSKLKEDK
                     LAELFGLA"
BASE COUNT         2973 a         3062 c         1966 g         3477 t
ORIGIN      
        1 tttttatatt ttcaccctaa tctccactaa attcccaaca accatccagt ctatctgaca
       61 ccggaattcc aaccatcctc cccatctatc cttcctatat tgtagcaccc acataccata
      121 tatacacaac ccccccagct tttccgtccc attcatacta tatataggaa acttttctat
      181 tccgccaaac taacaaacca ccatcccgct tctctttcag ccatctctgt cacagccaac
      241 agccagcaag ctcaaatcat tctcttcggc gacatttgca gggcctttag aaaaacttag
      301 ccccgggaaa tctgcgttat tcgcagatca ctcactgtct gagccgcttc gcggcgagtt
      361 tgagttatct gtgaatgctt ttagcagatt ttccggggct tagcttttct taagtcccga
      421 aaccgagccg aagagaatga tttgagcttg cgtccctctt acctgatctt cccgagctta
      481 gtttttctta agacccgaag tcaagccgaa gagaatgatt tgagcttgcg tccctttcat
      541 ctggaataaa acccactctc aacctccacc ggaatcaccc tttcctcaat cccctcaatc
      601 tcaataaacc tctgccgccc caacatctcc accatctgcg caagcagctc cctgctcccc
      661 tgcgcctcca tctcaacgcg gtcatcccag ttatttctca cccaaccggt aacccccaga
      721 gaactggcaa tataacaggc ccgatagcga aatccaactc cctgcactct ccccgaaaac
      781 caataatgct tccttatcag attctctctc atctccgaac ccttcccatc ctgaacatca
      841 tgctttttcc agactccccg gcctttctca tactcctcag caatctcctc acctgtctca
      901 gaatcctgca gaaacgttct gtacttcttt ctcttaaaat caaatatact caacagcaac
      961 cctctctcac agctactgtg ccggcaactt gatttcccga acattctcat gtctttcctg
     1021 ccagcctgtt tcaggaatca ctctgccgcc acactacaat cacaccctga tcacaaatcc
     1081 gtcttttctc gctttcgctt tcggatattc cccggagcga taaccaagca cacaatgacc
     1141 aattccaaca taagactccg gcacacccca ctctgccttc aatgcctttc cttcttcaga
     1201 agcaaacacc tctcttgcac gatgaatcca acaggaatca accccaaggg catgagcagc
     1261 attcatcaga tttcccataa ccagacttcc attttccaca catgtaggca tattactgtc
     1321 tgcaaagacc acaacaactg tcggtgcccc atagaacgga tcactctcag tccccatcac
     1381 atccgcattc attttagaaa gcttcgcaac aagttccgga ttctgtgtta ctaccataag
     1441 tgtaccctgt tgtcccattc cgcttggtgc gtattcccct gcttcaagaa tttcattcaa
     1501 cacttcttcc ggcaccagat cttctttata accacgtaca cttcttcgct ccaaaagatt
     1561 ttttattgtt tcattcatgt ctcatatcct cctgtattta ttttcatcac tgtcaaagca
     1621 acattactgt ccaacacgtt ctcagcaaca cagccaggca acaatatctt cctatattat
     1681 agcactacat ctaccaaaac tcacctgtat ttcccataat tttcaggcaa ttttccttgc
     1741 ccctgcatca caactggtct ataataaaga acagactaac atgaggtata aactatatga
     1801 acaaaaaaga aatttctgaa atcaagaaac agttcactcc cgagaactgt tctctcaccc
     1861 gcatctgcgg atgttacgtt gacggagaaa agaataaaaa aaccgaactg aaagaagcat
     1921 ttctctcttt atcagaagaa gaaatgttca aatactttga aattttccgc aaatcccttt
     1981 ccggaacaat aggaaaaaat ctgatgaaca tggaatttcc tctcgagcag gaaactgagg
     2041 gcggaactca acattttctt atgaaactga gagaaagcaa actgaccgat gatgcactgg
     2101 tagaaagttt ctacgataag atcattgaaa actatgatta tcccgaaaac tactacatca
     2161 tcctgatcca tggagtatat gatatccccg gaaagtcttc cgacggtgca gaaatgtttg
     2221 atgcatctga tgagatttat gagcacatca tgtgctgtat ctgtcctgta aaactctcta
     2281 aggcaggtct ttgctacaat gccgaaacca acagcatcca ggaccgtatc cgcgactgga
     2341 tcgtggaact tccagatgtt gctttccttt tcccgcagtt taacgaccgc agcactgaca
     2401 tccatggtgt cctttattat agtaaaaatg caaacgagct tcgggatatt tttatcgatg
     2461 aactgcttgg atgtacagca cctctttctg caggaggaca gcgtgattcc tttaacgcac
     2521 tggttgaaga tactctgggc gacgactgca gatatgacac tgtattaagc attcacgaga
     2581 aattaaacga cctgatcgaa tcacagaagg atgagccgga acctgtagtt ctgaccaaat
     2641 ctgaggtaaa acgcctcttt gaagaatgcg gcgtagaaga tgaaaaacta cagtcatttg
     2701 atgaacaata cgagatcaca gccggggaaa aatcctctct ggttgcttcc aacatcacca
     2761 atacaaaaag attcgagatc aaaaccccgg atgttgtagt ccacgtagat cctgaacgtg
     2821 ccgatcttgt ggaaacacgc gtaattgacg gacgcaagtg ccttgtgatc ccaatggaag
     2881 gcgaagttga actgaacgga atccgcgtac atacaggaaa tgaaaatcct tctgatgacg
     2941 aattttatga caatacagat actgagacag aggagacatc tgatggagaa taatcgtaaa
     3001 ctcttatttt ttgacattga caaaaccctg ttaacccctt acccatggac agttccggac
     3061 agtgcgaaac aggccttaaa agaagcccgc tataatggtc atctcctttt catcaattcc
     3121 ggacgtacct atacaatgat tccggaaatc attaaagaaa tgggattcga cggctatgta
     3181 tgcggatgcg gcagccagat ttatatgaat gaagagcttt tgttttccag ttctattcca
     3241 aatccgcttt gccgtaaaac aacagaatta ctgcgggaat gcagaattcc tgcgtttttt
     3301 gaacgcccgg ataagatttt atatgacagc aattcatgtg ctgttcctga aactgttcag
     3361 aagctgaaag cagaagctat agtagaagat ctggctctct acccgcccga aacatacaac
     3421 cattttacat ttgacaagtt tctggctttt ccgtctgctg attctgatat ggaaactttc
     3481 cgcaaatttg cagatgaaca ttttctgtgt ttcatacatg aagacaaggc ctgggaactg
     3541 actcagaaaa atctcagcaa agcaaccgga atccggtttc tggcagaccg ccttggggtt
     3601 ccggtcaaag acacctatgc attcggcgac agcacgaacg atcttcttat gctgcagttc
     3661 gccggaaaca gcatcgcaat gggcgactcg gatcccctga tcctgcctca ctgcacttac
     3721 cagacaacca atatcgaaga taacggaatt gccaatgctt tgaagcattt ccatctcatt
     3781 tagcagaaca tacaaaataa cgtattatca tatctctatt tatctgtgtt atttttgcat
     3841 atttaggaga atgatttcgg atttatttaa acttaaaaac agctatgtga aaacactcag
     3901 gttttcatca tagctgtttt aatttacatt aatcagttta cattaatcaa ttcccatcag
     3961 caggctactg acagttcgga ttttcttatt tagctgcttc tgtcggtgtt gcttccggtg
     4021 tttcagtagt ttctgctgct tcttcctccg ccgccggagt ctctgttgtc tctgtatctt
     4081 ctgctgcagg tgtttctgta acttcctctg tttccggtgt tggagttgcc tcttctgtca
     4141 cttcaggtgt cggtgtttcc ggaactgggg taggagtcgg tgccatgaag gtatgtttgt
     4201 cagtgatctt aagtgtctta atgactttct taactacctt tacatctgcc tcatccagcc
     4261 atttagcagt tgtatctgta aaataggtac tttctttgga attctgaaga gattctcgct
     4321 tggaagctgt ggcatcttca tcattaatct tgtcaagtct tactacatag tatcctgtgt
     4381 cagtctcaat aatattgtct gcaacttctc cgtccttcag tcctcgcaga accttcagaa
     4441 cctcatccgg atatgcctcg ctgccggaat ctttatcctc atctttgctc tcttttgttg
     4501 taaaattacc ctgtactgca gaataactgt catctacacc cttggcaatc tcactcatat
     4561 ctgctgtagg atcctctttc atcttatcca gaatctcctg agcctgttct ttcttagtct
     4621 ttttctcctc atcagttata tcatcaccgc ttgtagagat acttacataa gtgaaggttg
     4681 tctgattcgc ttcatcatca gaaactgtga tctttccttc tgctacaacc ggatcataca
     4741 tcttcttctg aattgtctga agctccagaa gtgtttttac ctgatcttcc gttaccgcaa
     4801 gttccttaat tgtctcttca ctgtttgcag ccataaactg tgatgctgca tctgcgatcg
     4861 ctgcttcatc atcatctgta agctctacgt tataatccgc agccttctct ttgagaatat
     4921 acatcttctc aacagactcc agactgcttg tcactgcctg atcaccataa gtctcatctg
     4981 aatcatcacc tgctgtctga tcccagatat tatctgcact tcccatataa tttaaataca
     5041 tggttgttgt ctgctgttgt tgttcgcggg cataaagact tacaactcca agcggaatat
     5101 ctgttccgtc aacagtagcc actgtctttg ttccatctaa tgttttactt ccacagccgg
     5161 aaagcatacc aactgacatc acacctgcca gtgtcacaac ggctgttctc tttaccattt
     5221 ttttcatttc ttcctccata ttatcgatct ggtctttctt ccataccact ttcccagaat
     5281 gtactgttat tagagtataa acttttttcc tgcgaagggc aaggcaaatc gaaaaatatg
     5341 tagtatattt cacatatttt ccgccatgct cttaagttcc tctgcaaatt ccgtcaatgc
     5401 caggatcaga ttcccctgtg gttccaggat aaatttcggt tcctgctcat tcttaaactg
     5461 cagacctctt ctgtatttct gcataagagc cggaaagcct tctgtattca gccttgcttt
     5521 ctcataaagg gtaaaccgga ctgtttttcc agtctgcttt atttccgtta cgtacccctg
     5581 atgagcaatc gcctttaact tcgcaatcgc cagaagattc agcactgcct ttccgggttc
     5641 tccgaaacgg tccagaagtt cttccagcat atcatcataa tcctgttgtg tctcaatccc
     5701 cgcaattctt ttataaatat ccagcttctg aaattcatta ctgatgtagg aatcaggaat
     5761 atacgcatca acattcagat caaccgatgt ctcgaaatcc tccattgtat gaataccctt
     5821 cgcctctttc actgcttcat tgagcatctt acaataaaga tcatatccaa ccgcattcat
     5881 atgtccatgt tgctgcgcac ccagcagatt tccggcacct cgaagttcca gatctctcat
     5941 tgcaatctta aatccgcttc ccagatccgt atactcgcgg atcgcagcca gacgcttctc
     6001 agccgtctcc ttcagcatca cattctttct gtacatcaga aacgcatagg cagttctgtt
     6061 ggaacgcccg atccttcccc gaagctgata aagctgagaa agcccgtatc tgtctgaatc
     6121 atggataatc atagtattta cattggaaat atccagccct gtctcaatga tcgtggtgga
     6181 aaccaacaca tcaatatccc cgtttacaaa ggaatacatc acattctcca gttccctctc
     6241 actcatctgg ccatgagcaa aatccaccct tgcgtccggc accagcttcg caatacgcag
     6301 ggcaacatct gcaatatccg tcactcgatt ataaacataa taaacctgtc cgcctctgcg
     6361 caattccctg ttgatagcct ctcggactgt ctcttcatca tattccataa catatgtctg
     6421 gatcggcatt ctatccatag gaggttcctc caggacactc atatcccgga ttccgatcag
     6481 gctcatatga agagtacggg gaatgggcgt tgcagtcagt gtaaggacat ccacatcctt
     6541 ctttaactgc ttgatttttt ccttatgcgt aactccaaac cgctgttcct catcaacaat
     6601 caacaggcca agatttttga actgtacgtc tttggaaagg attctgtgcg ttccaataat
     6661 cacatccacc tgaccttttt tcagatcttc gattgccttc ttctgttgtg ccggggtccg
     6721 gaatctgcac aacagttcca cacgtaccgg aaattccttc atcctctgca caaaggtatt
     6781 ataaatctgc tgcgcaagga tcgttgtagg tgcaagataa gctacctgtc tgctctcctg
     6841 tacttcctta aatgcggcac gcagcgccac ttctgttttg ccgtatccca catccccgca
     6901 gataagccgg tccataatcc tggtactctc catatcacgt ttggcatcct caatagcact
     6961 gagctgatcc tctgtctctt catatggaaa catttcttca aattctctct gccatacagt
     7021 atcaggacca cagacatatc cttccttttc ctgacgcact gcataaagct ctaccagttc
     7081 ttttgcaata tttttaactg ccccacgaac cttgctcttt gttttattcc actcctgagt
     7141 accaagtttg ttcagcttcg gagcctttga agcatctgcc cctgaatatt tctgcagaca
     7201 gtcaagctga gtagcaggaa tatagagatt gcttccgcct ctgtattcaa ttttaatata
     7261 atctttaacg atcctgtcta cttcaacttt ttcaattcct cggtaaatac caagaccatg
     7321 cttttcatgg acaacaaaat cgccgataga aagctccgca aaatcctgaa tacggcttcc
     7381 actgtaattt tttttcttct ttttcttctt ctgttcctgt ccaaaaatgt cagattctgt
     7441 cattaccgca aacttgatca acgggtattc aaagcccttc tttgcatggc cataaaccac
     7501 catgatctcg cccggacaga tctccctgtc ataatcttgt ccataaaagg ctgccagtcc
     7561 ctcttcctga agatcctttg ccagcctctc ggcccttgtg cgggaaccgg aaagcaacgc
     7621 gatcctgtat ccctgtttct tatactgatg aagatctttt accagaagtt caaaactgtt
     7681 gttataagcg ctgattgatt ttacctccag gtaaaatttc tcccgcactt tccagcctgc
     7741 ctgtttcggt tccagcgcac atacagaaat acagttgcgt ttattcagtt ctttttgaag
     7801 ctgttcaaaa gaacaaagcc agttttccgg cagattccgg ctgccttttt cctctctgtg
     7861 catacggctc tgtctgtact cttcttctac agccccgcct ttctcagtca gtcggtttgg
     7921 ttcatccaga aagattatgg ttctttcctc cggaaaataa tccaggaagg aaaccatatc
     7981 cttgtcccct atatgctcca ccgccggata gatcgtaagt tcttccagat tttccagaga
     8041 acgctgactc tccggatcaa aactccggat agaatcaatc tcatctcccc aaagctcgat
     8101 tctccatgga ttttcctcag tgagacagta aatatccaca ataccgccac gcacagagaa
     8161 ctgccccggc atttccacct gtcctacccg ctcgtaacct aaagccacca gctggttctt
     8221 cagctgttct gtgtctacgg tgctgtcact ttcgtaatga atcagctgtt cttttatctt
     8281 ttccagagat tccagaaaat ccatacatcc gtcaatactt gtaacaactg tcagttcttt
     8341 ctcttccagt aacgccttaa tgaccttcat cctctgacgt atcagcagat ttccatggat
     8401 atctgcctga aagaacagaa gatctttcgc aggataagaa tacacttttc tgtcataaaa
     8461 tctgtaatct tcataaattt ctctggcgcg gcgttcgtcc tccgccagga tcagacgata
     8521 aggcgcaatc ccggaaagtc cgtacatcag atgagatttc tgcgattcca tacaccccga
     8581 aacctgcagg attcccttat tcgttttgct tttttcttta atctgttcaa actctgcaag
     8641 cccctgcaga ggtgttaaaa atgctttcat cttcacccct catgatcctt cgacgtaata
     8701 aaagcggctt ctgtttccag ttaccgcttt ctataccttt tgctgaacaa tcatctgttt
     8761 cctgtctgct tatttctgta caggtacttt tctgttatat tcgttcatgg ctgcatctgt
     8821 accctgtgag attatgatat ccacagcctt tgccgccttt gttattgctt catccacaag
     8881 tttcctgtct tctgtggaaa atctgccaag tacatgatct gccagatccc atcccttcgg
     8941 ctttgcacca actcctactt tgatacggag aaatttctct gtgccaagct ggcggataat
     9001 gtctttgatc ccgttatgtc cgccggcact tccctgcttg cggatgcgaa gctgcccggg
     9061 ttccagatca atatcatcat aaataacaat aagctctgat tccggatcaa ttttaaaata
     9121 attcgccatc tcacgaacag gaccgccact caggttcata aaagaaagcg gcttcataag
     9181 aatcactttc tctccgccga tcatggtttt accgtacatg gcattaaatt tcacaccacc
     9241 ctgagggaca ttgtaatcct ctaccagacg gtcaacggta tcaaatccca tattatggcg
     9301 tgttgcttca tactgtttcc cgggatttcc caggccaact actaaataca tatatgtgtc
     9361 tcctgcttaa tttaacacca ttttccggaa ttaccatacc tgtcctgatg tcaaatttat
     9421 ttttttacat cctctaaagt gtgttcaaaa accaatcctg caaattgtaa cgcacgtctt
     9481 gcggaaagca tttttcaaac acacgctaag ataattaatt aaaatatacc atctcttcct
     9541 taagcggctc gcattctttc acatcacttg cataaagaac aatgccctca ccctgatatt
     9601 ctgtacggtt ctcaaatgcg accttcgctg ttacttctac ccagctgccc tctttgatat
     9661 gcggtgcata atcgcttctg cacacgtatc caaggaaagt ggtatcatct gcgcagcagg
     9721 tcattgccac acgtcccgga acaaagaatt tacttcccat tccacgtggt ttcatgacac
     9781 gtcctttaaa gcggacaatt ttgtctacat atttatccgg attatccatg gcatccacaa
     9841 accagatacc ataatcttcc ggtggaatct caatgatcgg tgcattgata tcaaacggca
     9901 tctcatcctg gaaaatgtca tccatctcgc cctcttcatt ttcaaagata acctctgcac
     9961 tctgatttgc gaccttgatc cctcgtctgt atgcaggaag cggatctccc tctttacagc
    10021 ggttaaatac aaccatatct gtatttctga tcatatccat gaacagagat ttcatgtttg
    10081 ccatataaac ctgataagta gttgcatcca cacaggtaat ctcctgctca atgccccagc
    10141 cttcaggaag ctccatctgc tcaaacttac ttacaagcca cattccgttg tactcgatca
    10201 caacacgctc cggctgatgg atgatatcca tggcagccag acgctgtggt gtcagatctt
    10261 cttcatcctc gatcacttcc acaactgtgt ttgtaagttt cagtttatcc atatcatatt
    10321 cttcttcgcc ttcttcgcaa agaatcaaca gagttttgcc gtcaatggca aaatactcct
    10381 gctgcaatgt aaaatccagg aaagtagtct ttccgctttc caggaatccc gtaaccaaat
    10441 aaatgggaac tctgatatca tccatcatat aatttattcc tttatcttta ttctgattct
    10501 gttattatct gccgaaatcg ttcagtctcg catctatgac acaggatttc atgctccgat
    10561 gagctgaatc cagatgcagt attttagaag attgccataa atcgctgctt cctcatgatc
    10621 attatgcaag cccaaataat tctgcaagtt tatcttcttt cagtttagag ccgattacgc
    10681 agagacgacc tgtatattcc ggtgcacctt cacggatttc tgtttcctcc ggaaccatgt
    10741 caaaataaat ccatgttccg tcttctgcag gaagcattcc ctttgcacga agaataatac
    10801 cgtaatcttc agaagcggaa agagcctcaa gcatcttctc aagaccttct cttgtgtatt
    10861 tcttaatcgt ctcacgtccc cagcttgtaa atacctcatc tgcgtgatga tggtgatggt
    10921 catgatgatc atgtccgcaa ccgcattcat ggtcgtgatc gtggtgatga tgctcatgtt
    10981 cctcatgatc atgtccacag ccgcattcat ggtcgtggtc atggtgatga tgttcatgtt
    11041 cttcgtgatc atgtccacag ccacattcat gatcatggtc gtgatcgtga tgatgatgct
    11101 ctccatgttc atgtacatgt ccgcactccg ggcatacctc ttcctcagac atcagttctt
    11161 cctcaagaga taccggtttc tccataacct caaggatttt ctttccgtcc agtttctcaa
    11221 tcggagtggt gataatagca gctttcgcat taattccacg aagaagttcc attgcctgct
    11281 gtgctttctt ctcatcaatg atatctgtac ggctcatgat gatcgcacct gcatattcaa
    11341 cctgattatc aaagaactca ccaaagtttc tcatatacat tttgcacttc tttgcatcaa
    11401 ctacagttgt atagctgttc agaacgatat ccccatctcc ctgaacaccc tgtactgcct
    11461 taataacatc tgagagtt
//
LOCUS       DBMDXG010000015        11434 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1610_length_11434_cov_2.233919, whole genome shotgun sequence.
ACCESSION   DBMDXG010000015 DBMDXG010000000
VERSION     DBMDXG010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1610_length_11434_cov_2.233919"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(370..1074)
                     /gene="deoD"
                     /locus_tag="ACZPD3_01030"
     CDS             complement(370..1074)
                     /gene="deoD"
                     /locus_tag="ACZPD3_01030"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR1414635.1"
                     /translation="MSIPTPHNSAKKGDIAKKVLMPGDPLRAKYIAETYLENPVCFNT
                     VRNMFGYTGTYKGEQISVMGSGMGMPSMGIYSYELYNFYDVEKIIRIGSAGALQDDVS
                     VMDVVIAMSACTDSNYASQYQLPGTFAPTASYELVARAVEVAKEQGTPVRVGSVVSSD
                     AFYGDNPESSKAWRKMGVLCVEMECAALFMNAARAGKEALGILTISDHVFRDEAISAE
                     ARQTSFNRMMEIALGI"
     gene            complement(1113..1877)
                     /locus_tag="ACZPD3_01035"
     CDS             complement(1113..1877)
                     /locus_tag="ACZPD3_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414636.1"
                     /translation="MDIQKFQQKLTEVCELGEKNGKVLKPEQIKECFGELDLDKSQLI
                     KILQYLKLKGISIEGAEEISAASQTGPEEVSEEKEEKVPLTAEEEAYLKEYLEGLEEQ
                     EQGERSAEELFELLSKGDALAQAELSQKYLHAAAEMAVEMNCEEIFIADLIQEANISL
                     LMALGEEEPEEKDEKWLLGRIRCGIRHAIEEQTQRKFEDDYLVAKVEKLEAAVRELTE
                     DEEDESSKFSVEELAIILDMDEEEIRDVLRLTGDDK"
     gene            complement(1977..2057)
                     /locus_tag="ACZPD3_01040"
     tRNA            complement(1977..2057)
                     /locus_tag="ACZPD3_01040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2021..2023),aa:Leu,seq:tag)
     gene            complement(2163..2235)
                     /locus_tag="ACZPD3_01045"
     tRNA            complement(2163..2235)
                     /locus_tag="ACZPD3_01045"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Lys,seq:ttt)
     gene            complement(2246..2317)
                     /locus_tag="ACZPD3_01050"
     tRNA            complement(2246..2317)
                     /locus_tag="ACZPD3_01050"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2283..2285),aa:Gln,seq:ttg)
     gene            complement(2327..2400)
                     /locus_tag="ACZPD3_01055"
     tRNA            complement(2327..2400)
                     /locus_tag="ACZPD3_01055"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2364..2366),aa:His,seq:gtg)
     gene            complement(2406..2479)
                     /locus_tag="ACZPD3_01060"
     tRNA            complement(2406..2479)
                     /locus_tag="ACZPD3_01060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2443..2445),aa:Arg,seq:tct)
     gene            complement(2501..2571)
                     /locus_tag="ACZPD3_01065"
     tRNA            complement(2501..2571)
                     /locus_tag="ACZPD3_01065"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2537..2539),aa:Gly,seq:tcc)
     gene            complement(2607..2681)
                     /locus_tag="ACZPD3_01070"
     tRNA            complement(2607..2681)
                     /locus_tag="ACZPD3_01070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2644..2646),aa:Pro,seq:tgg)
     gene            complement(3124..3603)
                     /locus_tag="ACZPD3_01075"
     CDS             complement(3124..3603)
                     /locus_tag="ACZPD3_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1414637.1"
                     /translation="MKVLIVSDTHGQDENLEETVLREAPFDYLIHCGDVEGREIFIEA
                     LAECPCTIVAGNNDFFTDLPYEEEVTLEGHKILVTHGHHYFVSRDYDKLVENAQAKGC
                     KIAMYGHTHMPVIENEDGILVINPGSLTYPRQRGRRPSYAVMQIEEGKDPQVEIRYL"
     gene            complement(3600..4184)
                     /locus_tag="ACZPD3_01080"
     CDS             complement(3600..4184)
                     /locus_tag="ACZPD3_01080"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HIR1414638.1"
                     /translation="MKKIIFATGNQDKMREIREILADMDVEVVSMKEAGIHADIVEDG
                     STFEENAVIKAKTICELTGEITLADDSGLEIDYLNKEPGIYSARYMGEDTSYHIKNAS
                     LIERLNGVPDEKRTARFVCAVAAAFPDGSVKTVRGTMEGRIGYEEKGENGFGYDPIFY
                     LPEYGCTSAELSGEEKNKISHRGKALRAIKDELK"
     gene            complement(4235..5386)
                     /locus_tag="ACZPD3_01085"
     CDS             complement(4235..5386)
                     /locus_tag="ACZPD3_01085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR1414639.1"
                     /translation="MEKFELIAPCHFGMEAVLKREILDLGYEITKVEDGKVTFEADAQ
                     GIADANIFLRSTERILLKVAEVKAETFDELFEKTKALPWENYIPKDGKFWVAKANSVK
                     SKLFSPSDIQSIMKKAIVERLKGVYGVSWFEEDGASFPIRVAFMKDVATIGIDTSGVS
                     LHKRGYRKMTVKAPITETLASALIMLTPWNKDRILVDPFCGSGTFPIEAAMMAADIAP
                     GMNRSFLAEEWKHLVPRKCWYDANEEAQDRINLNIETDIQGFDIDPEALKAARANAKM
                     AGVDKLIHFQQRAVKDLRHPKPYGFIITNPPYGERLEEKENLTQLYREIGESYERLDK
                     WSMYLITSYEKAENDIGRKADKNRKIYNGMLKTYYYQFLGPRPPKKNRS"
     gene            complement(5420..5962)
                     /gene="yfcE"
                     /locus_tag="ACZPD3_01090"
     CDS             complement(5420..5962)
                     /gene="yfcE"
                     /locus_tag="ACZPD3_01090"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR1414640.1"
                     /translation="MKYMFASDVHGSAYYCRKMLDAYKEEKAERLVLLGDLLYHGPRN
                     DLPREYAPKQVISMLNDMKKEIYAVRGNCEAEVDQMVLQFPVMADYCILNLDGRTFYA
                     THGHIYNENNLPPIQEGDILIHGHTHVLKAEQKEGYVLLNPGSVSIPKEGNPPTYAIF
                     EDGVFTIKDFEGNIVKSIEL"
     gene            complement(6063..6374)
                     /locus_tag="ACZPD3_01095"
     CDS             complement(6063..6374)
                     /locus_tag="ACZPD3_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR1414641.1"
                     /translation="MFPVETISAKMLDYYVGRRDTLIIDLREKESYMHSHVKGAVNMP
                     YGEIDGYTAFPKGKILVLYCDRGGASLLLARQLAKLGYCTRSVIGGFGAYRGRNLVIS
                     P"
     gene            complement(6500..6697)
                     /locus_tag="ACZPD3_01100"
     CDS             complement(6500..6697)
                     /locus_tag="ACZPD3_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414642.1"
                     /translation="MAKNYESESKNSRNYTGTSDKNSYGNEMNHDGNAKNSVKSKNET
                     DCRSKAKNKTSQSYSSEEDRY"
     gene            complement(7142..8746)
                     /locus_tag="ACZPD3_01105"
     CDS             complement(7142..8746)
                     /locus_tag="ACZPD3_01105"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1414643.1"
                     /translation="MGAAEAKAAILANKTALGIEFGSTRIKAVLVDDKNQPIASGGHE
                     WENRYENGVWTYSLDDIWTGIQDCYQDMARDVKAKYDIELESVGAFGVSAMMHGYMPF
                     NKEGELLVPFRTWRNNITGEASEKLMELFNYNIPQRWSIAHLYQAILNGEEHVKDIDY
                     ITTLEAYVHWKLTGKRVLGIGDAAGMFPIDTTKADYNQEMVDKFDELVAPYGFSWKLR
                     DIMPKALVAGEDAGVLTEEGAKLLDVTGKLKAGIPMCPPEGDAGTGMVATNSVAVRTG
                     NVSAGTSVFAMIVLEKELSRPYKEIDMVTTPSGHLVAMAHSNNCTSDLNAWVNVFKEF
                     AEAMGMEVDMNKLFGTLYNKALEGDPDCGGLLSYCYFSGEHMTGFEEGRPLFVRSPES
                     KFNLANFMRTNLYTCLGAMRVGLNLLFEKENVKVDRLLGHGGLFKTKGVGQQILADAV
                     NAPVSVMATAGEGGAWGIALLASYLVHKEEGETLESFLDNKVFADQESSTLDPKPEGV
                     AGFNAFMDSYMKGLSIERAAVESEIW"
     gene            complement(8973..10472)
                     /gene="araA"
                     /locus_tag="ACZPD3_01110"
     CDS             complement(8973..10472)
                     /gene="araA"
                     /locus_tag="ACZPD3_01110"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR1414644.1"
                     /translation="MKTGRNYKFWFCTGSQDLYGDECLRKVAEHSRIIVEELNKSGVL
                     PFELVWKPTLITNELIRKTFNEANADEDCAGVITWMHTFSPAKSWILGLKEYRKPLCH
                     LHTQFNEEIPYDTIDMDFMNENQSAHGGREYGHIVTRMGIERKVIVGHWADKDVQERL
                     ASWMRTAVGIMESSHIRVCRVADNMRNVAVTEGDKVEAQIKFGWEVDAYPVNEIADYV
                     KDVAKGDVDALVDEYYSKYDILLEGRDPEEFKRHVAVQAQIEIGFEKFLEEKNYQAIV
                     THFGDLGALKQLPGLAIQRLMEKGYGFGAEGDWKTAAMVRLMKIMTAGVKDAKGTSMM
                     EDYTYNFVPGKEGILQSHMLEVCPSVADGKIGIKVCPLSMGDREDPARLVFTSKTGPG
                     IATSLIDLGDRFRLIINDVECKKVEKPMPKLPVGSAFWTPQPNLKTGAEAWILAGGAH
                     HTAFSYDLTAEQMGDWAAAMGIEAVYIDKDTNIRDFKNELRWNELAFRK"
     gene            complement(10522..>11434)
                     /locus_tag="ACZPD3_01115"
     CDS             complement(10522..>11434)
                     /locus_tag="ACZPD3_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1414645.1"
                     /translation="VEAFHGRGTYCSENMRHIKNSKNIAVVTTYISDYIFPRLIQGMD
                     NVLSESGYSIILKNTANSRQKEARCLEELLKKDIDGLIIEPSKSEMICKHRNLYQNLD
                     KFEIPYVFIQGIYSEMRDKPHILMDDAQGGYLVTKYLLELGHKKIKGFFKADDMQGLE
                     RHKGYVKALQESGIAYDPDDVVWFHTEDRKVKPALMAKEMVQSGQLPDGIVCYNDQIA
                     VQVMEELEKMGVRIPDDISITGYDNSLYAQRGSGITTIAHPQEKLGEMAAELILEKIN
                     GVPEEESKVERLIYPELIIRGSCRKIL"
BASE COUNT         2931 a         2910 c         1960 g         3633 t
ORIGIN      
        1 ttgagaacag tcgtcattaa ctgttactgc tcactccgtt ctcagtaacg tagccaaaat
       61 ttcattcatt ccatatgaaa aaacagcgtc aaaaaatatt tcatttcatg tgcatttgca
      121 ggatacttag atattcttag ccgccaaaaa tctgcgttat gagccatcaa ggacactgtc
      181 tgagcgaagc gagtttgtcc ttgatggctc atgcttttag cagatttttg acggcttaga
      241 atatcttagt gtccgaaacc gcacatgaaa tgaaatattt tttgacgcgt ccctactgaa
      301 ttaaaaaaag ccctctgacc agcattccca cataccagcc aaagaacctt tttataccag
      361 gattatcttt taaattccta aagcaatctc catcatcctg ttaaacgaag tctgtctggc
      421 ctccgcagaa attgcctcat ccctgaaaac atgatccgaa atcgtaagaa tccccaatgc
      481 ctccttaccg gctctcgcag cattcataaa cagcgccgca cactccatct ccacacagag
      541 aacccccatc tttctccatg ccttcgaact ctccggatta tctccataga acgcatccga
      601 agatacaaca gaacccacgc gaaccggtgt accctgctcc ttcgcaacct ctacagccct
      661 tgcaaccagt tcataacttg ctgtaggagc aaaagtcccc ggcagctgat actggcttgc
      721 ataatttgaa tccgtacatg cactcatagc gatcacaacg tccattacgc ttacatcatc
      781 ctgcaacgca cctgcagaac caatacgaat gatcttctct acatcataaa aattataaag
      841 ctcataactg taaataccca tagatggcat ccccataccg ctacccatga cagatatctg
      901 ctcaccctta taagttccgg tataaccaaa catatttctc actgtgttaa aacataccgg
      961 attctccaga tatgtctccg caatatactt cgcacgcagc ggatctcccg gcatcagaac
     1021 cttctttgca atatcacctt tctttgcact gttgtgtggt gttggaatac tcataatcac
     1081 cctctccttt cccgtaatgc tacatcactg cattatttat catcacctgt aagccgcagc
     1141 acatccctga tctcttcttc atccatatca agaatgatcg ccagctcctc tacactgaac
     1201 tttgaacttt catcctcttc atcctctgta agttcccgga ctgccgcttc cagcttctcc
     1261 acctttgcta ccagatagtc atcctcaaat ttcctctgtg tctgctcctc gatagcatgg
     1321 cggatcccac accggatacg tccaagcagc catttctcat ctttctcttc cggttcttcc
     1381 tctcccagag ccattagaag gcttatattt gcctcctgaa tcagatcagc gataaaaatc
     1441 tcctcacagt tcatttcaac cgccatttcg gctgctgcat gcagatactt ctgtgacagc
     1501 tctgcctgtg ccagagcatc tccctttgaa agcagttcaa aaagttcttc cgcacttctc
     1561 tctccctgct cctgttcttc aagaccttcc agatattctt tcagatatgc ctcttcctcc
     1621 gctgtcagag gaactttttc ttctttttct tcggatactt cttccggtcc ggtctgagat
     1681 gctgctgaaa tctcctcggc accctcaatg gaaatacctt ttaattttaa atactgtaat
     1741 atcttgatca gctgtgactt atccaggtca agttcgccaa agcattcctt tatctgttcc
     1801 ggtttcaata ctttgccatt cttctctcca agttcacata cttctgtaag tttctgctga
     1861 aatttctgta tatccatagt caaaatctcc ctgtcctgtt tataacttaa ctatacaaca
     1921 aaaaactatg tctgtaaaac aaaaaaagca ccgttttaac cggtactttt tctgaatgcg
     1981 gataacagga cttgaacctg cacgggaagc ccactggtac ctaaaaccag cgcgtctgcc
     2041 aattccgcca tatccgcatt ttctcatatt ctcatattct cagagtgtac ctgaaaaatc
     2101 attctcgcac tctgcaaaag caataaaata gcggaaacct cacagaagat ttccgctatt
     2161 aatgaagcat cggggattcg aaccccggac aacttgatta aaagtcaagt gctctaccga
     2221 ctgagctaat gctccatata aaagactggg ctagctggat tcgaaccagc gaatgcagca
     2281 gtcaaagtgc tgtgccttac cgcttggcga tagcccaaag gcgaagggtg gatacaggga
     2341 ttcgaaccct gggcctccag agccacaatc tggcgcgcta accaactgcg ctatacccac
     2401 cacagcgtgc ctgaagggat tcgaaccccc gacccacggc ttagaaggcc gttgctctat
     2461 ccagctgagc tacagacaca tctctctgta catacagaga agcgggtgat gggaatcgaa
     2521 cccacgtatc tagcttggaa ggctagtgtt ctaccattga actacacccg catttcgctg
     2581 aaacggtaat gttgttgtta cagcagtcgg ggtgacagga ttcgaacctg cggcctcctg
     2641 gtcccaaacc aggcgctcta gccaagctga gccacacccc gctgtttttc tttatttatt
     2701 tgtgcttcgt tgtccgacgc aagacatatt atatgttaag caaccccaaa tgtcaacacc
     2761 ttttttcaat tttttttgat tttttttaaa aaagtgattt tttatacctc aaaaagtccc
     2821 tgaaacccgc taaatacaaa gctttgcggg cttttcactt ttttcaaaaa aatcgttttt
     2881 tttgttcgca aaaagggatt ttttactggt ttttgagatt ttttgtaaaa aaatattttt
     2941 ttcgcacaat aaaaaaatct taactttttt aattttttct tctattcttt tactgtcacg
     3001 tatctgtaaa aagaggtatt gctgttatcc gatcaacaat acctcaaaaa tatacagtta
     3061 gatgagtcag gcggatattc gcagcctccg caagtaactt acctgattca gttaaataat
     3121 ttttcagagg tatctgatct cgacctgcgg atcttttccc tcttctatct gcataactgc
     3181 atagcttggc ctgcggcctc tctgacgcgg gtaggtaaga ctgccgggat taattaccag
     3241 aatcccatcc tcattctcga tcacaggcat gtgagtatga ccatacattg caatcttaca
     3301 tcccttagcc tgcgcattct caacaagttt atcataatcc cgggacacaa aataatgatg
     3361 tccatgtgta accagtatct tatgtccctc cagagtaacc tcttcctcat agggaaggtc
     3421 tgtaaaaaag tcattatttc ctgcgacaat cgtacatgga cactctgcca gtgcctcaat
     3481 gaaaatctct ctgccttcca catccccgca gtggatcaga taatcaaagg gagcttctct
     3541 gagcactgtc tcttccagat tctcgtcctg tccatgagta tcgctgacga tcagtacctt
     3601 catttcagtt catcctttat ggcacgtaat gcttttcctc tgtggctgat cttatttttc
     3661 tcttcgccag aaagctctgc agaagtacat ccatattccg gcaggtagaa aatcggatca
     3721 tagccgaagc cgttctctcc cttctcctca tagcctatac ggccttccat tgttccacgc
     3781 actgttttta cacttccatc cggaaaagcc gctgcaacgg cgcatacgaa ccttgcagta
     3841 cgcttctcgt ccggaactcc attcagtctc tctatcagac ttgcattttt gatatgatat
     3901 gaggtatctt ctcccatata acgggcagaa taaatgcccg gctctttatt cagatagtca
     3961 atctccagac cggaatcatc tgccagagtg atctcccctg tcagttcaca gattgtcttc
     4021 gccttgatca cagcattttc ctcaaaagta gaaccatcct ctacaatatc tgcatggatt
     4081 cccgcttctt tcatagaaac aacctctaca tccatatctg caaggatctc gcggatttct
     4141 ctcattttat cctgatttcc ggtagcaaaa attatttttt tcataatcct ttgtatcctc
     4201 tttttatttt gatttttcgc aattacagaa gcactcagct acggtttttc ttcggtgggc
     4261 gcggcccaag gaactgatag taataggtct taagcatacc attatagatc ttacggtttt
     4321 tatctgcttt tcgcccgata tcgttttccg ctttctcata agatgtgatc agatacatgg
     4381 accacttgtc aagcctttca tagctttccc cgatttcacg ataaagctga gttaaattct
     4441 ctttttcttc cagtcgttcc ccataaggag ggtttgtgat gatgaatcca tatggtttgg
     4501 gatggcggag atcttttacc gctctctgct ggaaatggat cagcttgtcc acacctgcca
     4561 tttttgcatt agctctggca gccttcaacg cctcaggatc aatgtcaaag ccctggatat
     4621 ctgtttcaat attaagattt atcctgtcct gtgcctcttc atttgcatca taccagcatt
     4681 ttctcggaac cagatgcttc cattcctcag caaggaagga acggttcatg ccgggtgcaa
     4741 tatctgctgc catcattgct gcttcaatag gaaaggtacc gcttccgcag aaaggatcta
     4801 ccagaatcct gtctttattc catggcgtaa gcatgatcag agcagatgcc agtgtttctg
     4861 tgatcggagc tttcactgtc attttacgat atcctctttt atgcagagat acccctgagg
     4921 tatcaatacc gatcgtggcc acatctttca taaatgccac gcggatagga aagcttgccc
     4981 catcctcttc aaaccaggac accccgtata cgcccttcag acgctctaca atggcttttt
     5041 tcataatgga ctggatatca gacgggctaa agagcttact cttcacagaa ttggccttgg
     5101 caacccagaa tttcccatcc ttgggaatat aattttccca tggcagggct tttgtctttt
     5161 caaaaagctc gtcaaaggtt tctgccttta cttctgctac tttcagcagg attctttctg
     5221 tggaacgcag aaagatatta gcatctgcga ttccctgtgc atctgcctcg aatgttactt
     5281 ttccatcctc tactttggta atctcatagc ccagatcaag gatctcacgt ttcagcacag
     5341 cttccatacc gaaatgacat ggagcgatca gttcaaattt ttccattctt tatcctttct
     5401 atatgtagat gggacttccc tataattcta tgctcttaac aatatttccc tcaaaatcct
     5461 taattgtaaa cacaccgtcc tcaaagatcg catatgtagg cgggtttcct tcttttggaa
     5521 tggaaacaga acccgggttg agaagtacat agccttcttt ctgctctgct ttcagtacat
     5581 gggtatgtcc atggatcaga atatctcctt cctggatcgg aggcagatta ttttcattgt
     5641 aaatatgccc atgtgtggca taaaaggttc ttccatccag gttcagaata cagtagtctg
     5701 ccataaccgg aaactgcagg accatctgat cgacctcagc ttcacaattc ccacgtacag
     5761 cataaatttc tttcttcatg tcattaagca tggaaatgac ctgtttggga gcatattctc
     5821 tgggaagatc atttctcggg ccatgataga gcagatcgcc cagcagtacc aggcgttctg
     5881 ccttttcttc tttataggca tccagcattt ttctgcagta gtatgcagaa ccatggacat
     5941 ctgatgcaaa catatatttc atagttttgt gtctcctctt ttattggtat gccgacagat
     6001 tgatttctga aataacctga agatataccg gttcgtattt tttctcttac accttatcat
     6061 gcttatggcg aaattacaag atttcttccc cgatatgctc caaaaccgcc tataacagac
     6121 ctggtacagt acccaagctt tgcaagctgc cttgcaagca gaaggcttgc cccgcccctg
     6181 tcgcagtaca gaaccagtat tttccctttt ggaaaggctg tatatccgtc tatttctcca
     6241 tatggcatgt ttacggctcc ttttacatga ctgtgcatat aggactcttt ttctctcaga
     6301 tctatgatca acgtgtccct tcggcccaca taataatcca gcatttttgc agaaatcgtt
     6361 tccaccggaa acatggcaga ctcctttttg ttatatcata tgatatcagg gacagatgtg
     6421 ccactgcctg acaaaaaaca gccgacacat aagttggctc acagcagatc aggaggggca
     6481 tttccccctc ctgatccaat caatatctgt cttcttctga gctgtaagac tgggaagttt
     6541 tattttttgc tttacttcta caatctgtct cgtttttgct tttcacactg ttttttgcat
     6601 taccatcatg gttcatttca ttaccatagc tgttcttatc tgaagttcct gtgtaatttc
     6661 tgctgttttt actctcagat tcatagtttt ttgccattat tattttcctc ctgatgagat
     6721 ttatctgccc ggatctcctg atactctgca aaatccggtt tttttcttcc acacttcatc
     6781 gctgaaagtg cttcacattg actattatcc caaaaaccgg ggggaatatt catcaaaata
     6841 tgttgctttt ttcaccaagc tatattataa tcatagctgt aaaaagaatt tacccttcaa
     6901 aaattttttt tacataaacc ccttatttaa ttatttatac tccccttgaa agtcccggca
     6961 gctcccctgc cggggctttc tctttgtatt tattcggctg cgaatcactt gataacaatt
     7021 tcagaaaaac tacaaaaaac agcatcgccg gtctcatgat ctgcgatgct gctctgtttt
     7081 ttatattctt cttttcctat tcattttcat atgcgccggc aaacagtttg tttctcaaca
     7141 tttaccagat ttcagactct actgctgctc tttcaataga aagacctttc atatagctgt
     7201 ccataaatgc attgaatcca gccacacctt ccggttttgg atccagtgtg ctgctttcct
     7261 gatctgcaaa tactttgtta tcaaggaagc tttccagagt ctcaccttct tctttatgta
     7321 ccagataaga agcaagaagt gcgattcccc aggctccgcc ctcacctgca gtagccatta
     7381 cagataccgg tgcattgaca gcatctgcca gaatctgctg tcctacgcct tttgtcttaa
     7441 ataatcctcc atgaccaaga agacggtcta cttttacatt ttctttctca aacagaaggt
     7501 tcagacctac cctcatggct ccaaggcatg tatacagatt ggttctcata aagtttgcaa
     7561 gattgaattt gctctcagga gaacgtacaa ataacgggcg tccttcttca aagcctgtca
     7621 tatgctcgcc tgagaaatag cagtaagaca gaagtccgcc acagtccgga tcaccttcaa
     7681 gtgctttatt ataaagagtt ccaaacagtt tattcatatc cacttccata cccatagcct
     7741 ctgcaaactc tttaaataca tttacccagg cattcaggtc agaggtacag ttgttggaat
     7801 gagccatagc taccagatgt ccggacggag tggtaaccat gtcgatttct ttatacggtc
     7861 tggagagttc tttttccaga acgatcatag caaatacaga agttcctgcg gaaacgttac
     7921 ctgtacgaac tgcaacactg tttgtggcaa ccatacctgt tcctgcatca ccttccggag
     7981 gacacatcgg gattcctgct ttcagttttc ctgttacgtc gagaagtttc gcaccttctt
     8041 ctgtaagaac accggcatct tcacctgcaa caagggcttt cggcataata tcgcgaagtt
     8101 tccatgagaa tccataagga gcaaccagtt catcgaattt atcaaccatc tcctggttgt
     8161 aatctgcctt tgtggtatca atcgggaaca tacctgctgc gtcaccgata ccgagaactc
     8221 tctttcctgt cagtttccag tggacataag cctccagagt tgtaatataa tcgatatctt
     8281 ttacatgttc ctcgccatta agaattgcct ggtacagatg tgcgatactc catctctgag
     8341 gaatattgta attaaacagc tccatcagct tttcactggc ttcccctgta atattatttc
     8401 tccatgtacg gaatggtaca agaagttctc cctctttgtt aaatggcata tatccatgca
     8461 tcatggcgct gacgccaaat gcacctacag attccagttc tatgtcatat tttgccttca
     8521 catcccttgc catatcctga taacagtcct gaatgcctgt ccagatatca tcaaggctat
     8581 atgtccagac accgttttca taacgatttt cccattcatg accgccagat gcgattggct
     8641 gatttttatc atctacaagt actgctttga tcctggtaga accaaattca ataccaagcg
     8701 ctgttttgtt agcgagaatc gctgcttttg cttctgctgc tcccattttt atgtacctcc
     8761 ctgtttctct gttatttatg attacagatc attcgacaat ctataatcat atattgtttg
     8821 tacaccaata ttattgcatt tcttttattg ttttaccatg gaaattttac ggttttaaca
     8881 tactttttat tactttaaac ttctcagggc tgccatccca ggcagttaat ttcagcctga
     8941 tatggcagcc tctgtattaa ttacaaaaat tattattttc tgaatgcaag ttcattccat
     9001 ctcagttcat tcttaaaatc acgaatattt gtatctttat caatgtaaac agcttcaata
     9061 cccatagctg ctgcccagtc acccatctgc tctgctgtca ggtcatagga gaatgctgta
     9121 tggtgggctc cgcctgcaag gatccatgct tctgcacctg tcttaagatt tggctgtggt
     9181 gtccagaatg cacttcctac aggaagcttc ggcatcggtt tctcaacctt cttgcattcc
     9241 acatcgttga taatcagacg aaaacggtct ccaagatcga tcagggatgt tgcaataccc
     9301 gggcctgtct tgcttgtgaa tacaagtctt gcaggatctt ctctgtcacc catggaaagc
     9361 gggcatactt taatgccaat cttaccatct gcgacagacg ggcatacctc aagcatatga
     9421 gactgaagga ttccttcttt tccaggtaca aagttatatg tatagtcttc catcatggaa
     9481 gttccttttg catcttttac accagctgtc atgatcttca tcagacgaac cattgcagct
     9541 gtcttccagt cgccttctgc accgaatcca tagccttttt ccataagtct ctggattgca
     9601 agtccgggaa gctgtttaag agcacccaga tctccaaagt gtgtaacgat tgcctgataa
     9661 tttttctctt ccaggaattt ctcaaatccg atctcaatct gagcctgaac tgctacatga
     9721 cgtttaaatt cttccggatc tcttccctca agaagaatat catatttgct gtagtattca
     9781 tctaccagcg catctacatc acctttagct acatctttta cataatcagc aatctcattt
     9841 acaggataag catcaacttc ccatccgaat ttgatctgtg cttctacttt atcaccttct
     9901 gtaacagcta cgtttctcat attatctgct acacgacata cgcggatatg gctgctctcc
     9961 atgataccaa ctgctgtacg catccaggaa gcaagtcttt cctgaacatc tttatctgcc
    10021 cagtgtccga caataacttt tctttcgatt cccattcttg taacaatatg accatattca
    10081 cgaccgccat gagcagactg gttttcattc ataaaatcca tatcaatggt atcataagga
    10141 atctcctcat taaactgtgt atgaagatgg caaagcggtt ttctgtattc tttcagacca
    10201 aggatccatg attttgccgg tgagaaagta tgcatccatg tgataacacc tgcacagtcc
    10261 tcatctgcat ttgcctcatt aaatgtttta cggatcagtt cgtttgtgat cagtgttggt
    10321 ttccatacta attcaaatgg aagaacacca ctcttgttta attcctcaac aatgattctg
    10381 gaatgctcag caacttttct taagcactca tctccgtaaa gatcctgtga acctgtacaa
    10441 aaccagaact tatagtttct tcctgttttc atgataaaat tcctcctgga tttattttta
    10501 ttttttattt atttcatttg tttacaatat ttttctgcat gatccccgta tgatcagttc
    10561 cggataaatc agtctttcaa ccttactttc ttcttcagga actccgttga tcttctcaag
    10621 tataagctct gctgccattt ctcccagctt ttcctgcgga tgagctatcg ttgtgattcc
    10681 gcttcctcgc tgagcataca gtgaattgtc ataaccggta atggaaatat catccggtat
    10741 cctgactccc attttctcca gttcttccat aacctgaaca gcaatctgat cattgtaaca
    10801 gacgattcca tccggaagct gtccgctctg taccatttct tttgccatca gtgcaggttt
    10861 tactttcctg tcctctgtat gaaaccagac aacatcatcg ggatcatagg caatccctga
    10921 ctcctgtaag gccttcacat atcccttgtg gcgctccagt ccctgcatat cgtctgcctt
    10981 aaagaaaccc ttgatctttt tatgtcccag ttccagcaga tattttgtta caagatagcc
    11041 tccctgtgca tcatccataa gaatatgagg tttatccctc atttctgaat agattccctg
    11101 aataaacaca tacggaattt caaatttatc cagattctga taaagatttc tgtgtttaca
    11161 gatcatctca cttttgcttg gttcaatgat cagtccatca atatctttct tcagaagttc
    11221 ctccagacat ctggcctctt tctgtctgct gtttgctgtg tttttcagga taatactgta
    11281 accgctttct gaaaggacat tatccattcc ctgtatcagt ctcggaaaaa tataatcaga
    11341 aatatatgtg gtcactaccg caatattctt tgaattcttt atatggcgca tattctcaga
    11401 gcaataagta cctcttccgt gaaatgcctc taca
//
LOCUS       DBMDXG010000016        11395 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1617_length_11395_cov_2.107933, whole genome shotgun sequence.
ACCESSION   DBMDXG010000016 DBMDXG010000000
VERSION     DBMDXG010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1617_length_11395_cov_2.107933"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="ACZPD3_01120"
     CDS             <1..509
                     /locus_tag="ACZPD3_01120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1414646.1"
                     /translation="ADTISGSEADFAELMNKKARKLGMENTHFCDSTGLHNPDHYSTV
                     KDIAVLMKYCIKNDTFREIVETSRHSTGVTNIHPDGITYYSTMFKNLSDPTVTGGKIL
                     GGKTGYTSEAGHCLVSFAEIEGREYIFVSAGASGADGNTIPHIQDAVTVYNRVGAAIE
                     AKMNDNNK"
     gene            506..694
                     /locus_tag="ACZPD3_01125"
     CDS             506..694
                     /locus_tag="ACZPD3_01125"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1414647.1"
                     /translation="MKSPTNCDAHLVGSIFVTVHTPLSREVFWHENAKIPRHTARIYA
                     NSINSVHREACYWARSEQ"
     gene            810..1112
                     /locus_tag="ACZPD3_01130"
     CDS             810..1112
                     /locus_tag="ACZPD3_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414648.1"
                     /translation="MTDRQMLEAVLTEVRGLGTRMDRMEERQKCFEEETRNNFADIRL
                     HLENITDRNISILAENHLNLINKMDVSATWMNRIMINEVKMNALTDQVARLQAKSS"
     gene            complement(1205..1750)
                     /locus_tag="ACZPD3_01135"
     CDS             complement(1205..1750)
                     /locus_tag="ACZPD3_01135"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HIR1414649.1"
                     /translation="MTKWVCSVCGYVYEGEKAPEACPVCKAPADKFVKQDGEMSWAAE
                     HVVGVAQGSSEDIMADLRANFQGECSEVGMYLAMARVAHREGYPEIGLYYEKAAYEEA
                     EHAAKFAELLGEVVTDSTKKNLQMRVEAENGATAGKTDLAKRAKAANLDAIHDTVHEM
                     ARDEARHGKAFAGLLKRYFGE"
     gene            complement(2011..2424)
                     /locus_tag="ACZPD3_01140"
     CDS             complement(2011..2424)
                     /locus_tag="ACZPD3_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR1414650.1"
                     /translation="MATLKYSRQRESIKEFLRTRTDHPTADVVYENMKLIYPNISLGT
                     VYRNLSLLADLGEIKKLSSFAGADHFDGRTERHCHFMCIRCERIIDLESEGIHHIMDL
                     AGENFKGKITDYSARFFGLCEDCLKETEGNSDSSK"
     gene            3328..5394
                     /gene="metG"
                     /locus_tag="ACZPD3_01145"
     CDS             3328..5394
                     /gene="metG"
                     /locus_tag="ACZPD3_01145"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR1414651.1"
                     /translation="MVLADFQTLRVPSSRNRTENNYIFRSVHIQKRRQQTMEKQKYYI
                     TTAIAYTSGKPHIGNTYEVVLADAIARYKRQQGYDVFFQTGTDEHGQKIELKAEEAGI
                     TPKEFVDNVSGEIKRIWDLMNTSYDKFIRTTDEDHEKQVKKIFKKMYAKGDIYKGHYE
                     GMYCTPCESFFTESQLVDGKCPDCGRPCVPAKEEAYFFKMSKYADKLIDYINTHPDFI
                     QPESRKNEMMNNFLLPGLQDLCVSRTSFKWGIPVDFDPKHVVYVWLDALTNYITGIGY
                     DCDGESTDQFNKLWPADLHLIGKDIIRFHTIYWPIFLMSLDLPLPKQVFGHPWLLQGD
                     GKMSKSKGNVIYADELVDFFGVDAVRYFVLHEMPFENDGVITWELMVERLNSELANTL
                     GNLVNRTISMSNKYFGGVVENKGVTEPVDDDLKNFILSVPAKVNEKMDKLRVADAMTE
                     VFTIFKRCNKYIDETMPWALAKDEEKKDRLATVLYNLVEGICIGAVLLKSFMPETTER
                     ILAQLNAQDRELEDLKTFGLYPSENKVTEKPEILFARLDLKEVLAKVAELHPPKAEEP
                     AKEEKEDVIDIEAKPEITFEDFGKLQFQVGKIIKCEEVKKSKKLLCSQVQIGSQVRQI
                     VSGIKAHYSAEEMVGKRVMVVTNLKPAKLAGVLSEGMILCAEDADGNLSLMVPEKEMP
                     AGAEIC"
     gene            5657..6133
                     /gene="moaC"
                     /locus_tag="ACZPD3_01150"
     CDS             5657..6133
                     /gene="moaC"
                     /locus_tag="ACZPD3_01150"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR1414652.1"
                     /translation="MSQGFNHFDENGNAVMVDVSGKNITYRTAVATGEIHVGEAIMEA
                     VKEGSVKKGDVLGVARVAGIMGVKRTSELIPMCHPLPIQKCSVDYELDETNGIIRAFC
                     TVKTEGKTGVEMEALTGVQVTLLTIYDMCKAIDKHMVMSNIHLVEKTGGKSGDFHF"
     gene            6259..6747
                     /locus_tag="ACZPD3_01155"
     CDS             6259..6747
                     /locus_tag="ACZPD3_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR1414653.1"
                     /translation="MKRVAIITSSDTGYRGEREDLSGPAIREIVEKNGYEVVSEDVLP
                     DDRKMLSERMAEIADSGTAELILTTGGTGFSPRDITPEATEDIIDRRVPGIPEAMRAY
                     SMTITKRAMLSRSTAGIRKKTLIINLPGSPKAVKESLEYIIDALGHGIEIMTGEAGNC
                     AR"
     gene            6914..7342
                     /locus_tag="ACZPD3_01160"
     CDS             6914..7342
                     /locus_tag="ACZPD3_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR1414654.1"
                     /translation="MGKVIAVCTSERKGIQKTSVPEIKVIEDWGIEGDAHAGKWHRQV
                     SLLSFDKIEDFRARGAEVEDGAFGENLVVQGIDFATLPIGTKFQCNDVVLELTQIGKE
                     CHSGCAIFKKMGECIMPKQGVFTKVLHGGVIHPGDELVIL"
     gene            7492..8265
                     /locus_tag="ACZPD3_01165"
     CDS             7492..8265
                     /locus_tag="ACZPD3_01165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR1414655.1"
                     /translation="MIIDTHAHYDDEAFDTDREALLMSMYDGGIEKIVNVCASVGGFQ
                     DTVKLMEKYPFVYGAVGIHPDDADKMTQETLDEIRRLSHMDKMVAIGEIGLDYYWHKE
                     EEEHQIQKKMFRAQLDIAREEKLPFMIHSREAAEDTLNIVREYMQGGMYGGIIHCFSY
                     SREIAAEYLKMGLYLGIGGVVTFKNAKKLKETVQYAPLSQLVLETDCPYMSPEPNRGK
                     RNSSLNLPYVAQAIAELKGITAEEVIDVTRQNAEKLLGV"
     gene            complement(8366..9769)
                     /locus_tag="ACZPD3_01170"
     CDS             complement(8366..9769)
                     /locus_tag="ACZPD3_01170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR1414656.1"
                     /translation="MKKNNMPLNLRERINSRISLLCSVIVLVLLVLVMHQMDYTLIRK
                     PQKEAAEAAALKEQQDKIKAETPVISTASVIAVGDNLYHSKLYESGENDSGIWNYDHI
                     YTHVLDQIQAADVAMIDQETVFAPSHDAVSTYPSFATPQEVGDAIIKAGFDVVESATN
                     HADDYGYDYLKSTLDFWSTNYPDIPVLGIHATQEDADTVKVKEVNGIKIAFLDYTYGT
                     NNSGAGEGYEYMIDIFDKDKITTMIQKAKEISDCIIFVAHWGTEDETMPNEYEKQWAA
                     FLMQQGVDVIIGGHPHVLQPYGQLSDDQGHNTTIFYSLGNFVSTQQELPELLEGMASF
                     TIQKSTLNGKSTIQILSPEVKPMVMHYNHDNGEYGPYMLDDYTEELASSHSVRNVIGD
                     EFTLDNLKAKFKEIMSMNVKPSTNTNLLNVKFDWEGNMIDKTTGNVVEDTESIHSWEY
                     QADTSDGTEDSSDDSGY"
     gene            10112..10549
                     /locus_tag="ACZPD3_01175"
     CDS             10112..10549
                     /locus_tag="ACZPD3_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1414657.1"
                     /translation="MNHYEAINDVLVNLFNEILDLEERALITGEYKNISVNDMHIINA
                     VGIREQKNMSTVARELNVTVGTLTIAVNNLVKKGYIQRMRSQEDRRVVLISLTEQGKK
                     AYYHHKDFHEKMVLAVLKGLNVEETEALTKALTKLQGFFRSYQ"
     gene            10561..>11395
                     /locus_tag="ACZPD3_01180"
     CDS             10561..>11395
                     /locus_tag="ACZPD3_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HIR1414658.1"
                     /translation="MEYTEKDLVKIAKRENNTKRNYLVVDPLQGKHIPVVPSKALDLF
                     AALADTFREKYKDEKLLLVGFAETATAIGAQAAITVGADYIQTTREVIPGVNYLFFSE
                     EHSHATEQKLVKDDIDRAAAETDRIIFIEDEVTTGKTIRNIISILDREYDGKFKYSVA
                     SLLNGMSEENLERYKRQGISLYYLVKTDHSTYGDRAETFKGDGFYYKCLDKVVEYTTI
                     YVKNRMDARRLIDSGKYEEACENLWREIREKTGNMADNISGKRILVIGTEEFMFPALY
                     IG"
BASE COUNT         3496 a         2206 c         2686 g         3007 t
ORIGIN      
        1 tggcagatac gatcagtggt tcagaggcgg attttgcgga actgatgaat aagaaagcaa
       61 ggaagcttgg aatggagaat acccatttct gtgactctac aggactgcat aacccggatc
      121 attacagtac ggttaaggat attgcggtgt tgatgaagta ttgcattaag aatgatacgt
      181 ttcgtgagat tgtggagacc tccagacatt ctaccggagt tacaaatatc catccggatg
      241 gaattactta ctacagtaca atgtttaaaa atctgtctga tccgacagtc acaggtggaa
      301 aaatcctcgg tggcaagacc ggctatacca gtgaggcagg acactgtctg gtgagttttg
      361 cggagattga gggcagggaa tatatctttg tttccgcagg tgcttcagga gcagatggaa
      421 atacgattcc gcatattcag gacgctgtga ctgtttataa cagggttggt gcggcgattg
      481 aggcgaagat gaatgataat aataaataaa atctcccaca aattgtgacg cacatcttgt
      541 ggggagcatt ttcgttactg ttcacacgcc actatcccgc gaggtgtttt ggcatgagaa
      601 tgccaaaatc ccgagacata ccgcgcgaat ttatgcgaat agcataaatt ctgtgcatcg
      661 cgaagcgtgt tactgggcac ggagtgaaca gtaacgcatt ttccaaatac actctaatgt
      721 ttatagattt ttatagacat atttttatgt atgtgatata atcaatcaca tacaaaagaa
      781 cgatacgggc tacagaggag gtgaaagtgg tgacagacag acaaatgctg gaagctgttt
      841 tgacggaggt cagaggactt ggaacgcgaa tggaccgaat ggaagagcgt cagaagtgtt
      901 ttgaagaaga gaccagaaat aactttgcag atatcagact gcatttggag aacataacgg
      961 ataggaatat ttctatcctt gcagagaatc atctgaatct tatcaataag atggatgtgt
     1021 cggctacctg gatgaacagg attatgatta atgaggttaa gatgaatgct ttgacagatc
     1081 aggttgccag acttcaggcg aaatccagtt aagcttccaa tgatgaaaat acgaacagat
     1141 aaaaagggag aaccattgag gttctccctt taaaaggttt attttatgcg attttccgat
     1201 agaattattc tccgaaatat ctcttaagaa gacctgcgaa tgcttttccg tgtcttgctt
     1261 cgtctcttgc catttcatga actgtatcat ggatagcgtc aaggttagca gcttttgcac
     1321 gtttagcaag gtctgtttta ccagctgtag caccgttctc ggcttctaca cgcatctgaa
     1381 ggtttttctt tgtgctgtct gttacaactt cacctaataa ttcagcgaat ttagcagcat
     1441 gttctgcttc ctcataagct gctttttcat agtataaacc gatttccgga tatccttctc
     1501 tgtgagcaac tcttgccatt gcaagataca taccaacttc agagcattca ccctggaagt
     1561 ttgctcttaa atcagccatg atgtcttcgc tggatccctg agcaacacct acaacgtgtt
     1621 cagcagccca tgacatttcg ccatcctgct ttacgaattt atcagccggt gccttacata
     1681 caggacaggc ttccggagct ttctcacctt cataaacata accacataca ctacataccc
     1741 attttgtcat aattaaaatc tccttttctt tttaatgaat ttattaggga tgccagaaaa
     1801 taaattgatt atctcagcat cgtttagttc ttactgcttt ccgtctgtgc ggcagaaaaa
     1861 gtaatttttt aataatagta attattactg ttttctgtga ttttagatta ccatacttga
     1921 ttgaaaaagt caagtttaat tttagctttt ttgcaacttt ttatcattag ttgttattcc
     1981 caatttggcc actaatgatg taagcaaaaa ctactttgaa ctatcagagt tgccttctgt
     2041 ctctttcagg cagtcctcac acaggccgaa gaatctggca ctgtaatcag taatctttcc
     2101 tttgaagttc tcacctgcaa gatccataat gtggtgaatt ccctcgctct caaggtcgat
     2161 gatccgctca catctgatac acatgaagtg gcagtgcctc tcggtccgcc cgtcaaagtg
     2221 atccgctccg gcgaaagagg aaagcttctt aatctctccc agatcagcca gcagagataa
     2281 gttgcgatat accgtaccga ggctgatatt tggatagatg agcttcatat tctcatatac
     2341 cacatcggca gtaggatgat ctgttctggt tcttagaaac tctttgatcg attccctctg
     2401 acggctgtat ttcagtgtcg ccatggtatt cctcccttcc ctgtttactc gcaaatgatt
     2461 tatcataact gttcaggata gttgcagtta taatctgttc aataacgata atcattactg
     2521 atattgatac tatacaatat tggattgaaa ttgtcaagat ataaaattat tattttatag
     2581 aattgttata gtttggttac tgagaacgga gtgaacaata acgatgtggg tttacaaatc
     2641 caccaaaatg tgctatattt tccctataac aggaaataac tatttcctat agctaacagc
     2701 gttccacgat cacagagcgg agtgctgtca cgaaataaca caaactcatt gaagaagaga
     2761 gtagatccgc agaccgtatt acagagaaat ccccatggat catatatcac agattcaaag
     2821 gctgagaggg atatgcaagg agcaggttga agatggcttc cgagaggcac tgctgagggc
     2881 caacagcttt aggcagcgac aggcacgcct gttacagcgt agaggtatca caacgtacct
     2941 cccaaggttt ccccgcgcga gcccggaaac gaacagaagt ggtaacacgg gtaatactcg
     3001 tcttctcaga taatctgagg agacgttttt tatgttatag gaaattactc tgtaatgttc
     3061 ctataacata aaaaagccct ccgggcagga ttacactgcg acagagagga attatttccc
     3121 caaacaccag ttccttccta ccatgaagaa tttatgtttt tcttccataa ataaggagca
     3181 gttgttacca tttttaaaca ccagcttcgg aaaatataat tgttttctgt gcatttgcag
     3241 ggagtgtagc aactcttagt gcctggaaat ctgcgttatt cgaaaaaatc tcactgtctg
     3301 agcgaagcga gtttgagatt ttttcgaatg gttttagctg attttcagac acttagagtt
     3361 cctagctccc gaaaccgcac agaaaacaat tatattttcc ggagcgtcca cattcaaaaa
     3421 aggagacaac aaaccatgga aaaacagaaa tactacatca ctaccgccat tgcctataca
     3481 tctggcaaac cgcacatcgg caacacctat gaagtagttt tagccgacgc cattgcccgc
     3541 tacaaacgtc agcagggcta cgacgtattc ttccagacag gaaccgatga acatggtcag
     3601 aaaatcgaat taaaagccga agaagccgga atcaccccaa aagaattcgt agacaacgta
     3661 tccggtgaga tcaaaagaat ctgggatctg atgaacacat cctacgacaa attcatccgt
     3721 accacagatg aagaccacga aaaacaggtc aaaaaaatct tcaaaaaaat gtacgcaaaa
     3781 ggcgacatct acaaaggaca ctacgaagga atgtattgta caccatgcga atctttcttt
     3841 acagaatctc agcttgtaga cggcaaatgt ccggactgcg gccgcccatg tgtacctgca
     3901 aaagaagaag catatttctt caaaatgagc aaatatgccg acaaactcat tgactacatc
     3961 aatacacacc cggacttcat ccagccggaa tcccgtaaaa acgagatgat gaacaacttc
     4021 cttctcccgg gacttcagga tctttgcgta tccagaactt ccttcaaatg gggaatcccg
     4081 gtagactttg atccgaaaca cgtagtatat gtatggcttg atgcccttac aaactatatt
     4141 acaggaatcg gctacgactg tgatggcgaa agcacagacc agttcaataa gctctggcct
     4201 gcagacctgc atctgatcgg taaagacatc atccgtttcc atacaattta ctggccgatt
     4261 ttcctgatgt cactggatct gccgcttccg aaacaggtat tcggacatcc atggcttctc
     4321 cagggtgatg gtaagatgag taaatccaaa ggaaacgtaa tctatgcaga tgaactggta
     4381 gacttcttcg gtgtagatgc agtccgctac ttcgtacttc atgaaatgcc attcgagaat
     4441 gatggtgtga tcacatggga actgatggta gagcgtctca attctgaact cgctaacacc
     4501 cttggaaacc ttgtaaaccg taccatttcc atgtcaaaca aatatttcgg cggtgtggta
     4561 gaaaacaaag gcgttacaga acctgtagat gatgatctga agaacttcat tctctccgta
     4621 cctgccaagg taaacgaaaa aatggataaa ctccgcgtgg cagatgcaat gacagaagta
     4681 ttcacaatct tcaaacgctg caacaaatac atcgacgaaa caatgccatg ggctcttgct
     4741 aaggatgaag agaaaaaaga ccgtcttgca acagttctct acaatctggt tgagggaatc
     4801 tgcattggtg cggttctgct gaaatccttt atgccggaaa caacagagag aatccttgcc
     4861 cagttaaatg cacaggacag agaactggaa gaccttaaga cctttggcct ctatccgtca
     4921 gaaaataaag taactgaaaa accggagatt cttttcgccc gccttgacct gaaagaggta
     4981 cttgcaaagg tggcagagct tcatccgccg aaagcagaag aacctgcaaa agaagaaaaa
     5041 gaagacgtaa ttgatattga agcaaaacct gagattacat ttgaagattt cggcaagctt
     5101 cagttccagg ttggtaagat catcaaatgt gaagaagtaa aaaaatcaaa gaaactgctc
     5161 tgctcacagg tacagatcgg cagccaggtg cgccagatcg tatccggaat caaagcacac
     5221 tacagtgcag aagaaatggt aggtaagagg gttatggtag tcacaaacct gaaaccagcc
     5281 aaacttgccg gtgtactcag tgaaggaatg atcctctgtg cagaagatgc agacggaaac
     5341 ctttccctga tggtaccgga aaaggaaatg cctgcaggtg cagaaatctg ctgatctttg
     5401 tattactgag aacaaagcga atggtagcga aaatgtgtga aaacttacga atattatctt
     5461 gacaaataca ctctcaaaag ctatattaat atttagataa aggggagtga ctgactcatg
     5521 agtattcatg gtgtcgtcaa tttcggtggg taaagactcc aaccgtatcg tgatgaacag
     5581 gcgaggcttt tattgtatgt tcccgtacaa taagagcctc gctttttgta aaaacttaaa
     5641 cagggggatt ttgatcatgt cacagggttt taatcatttt gatgagaacg gaaacgcagt
     5701 tatggtagac gtttccggga aaaatatcac ttacagaaca gcagttgcca caggcgagat
     5761 tcatgtagga gaagcaatca tggaggctgt gaaagagggt tctgtgaaaa agggagatgt
     5821 gcttggagta gcacgtgtag cggggattat gggagtgaaa aggacttctg agttgattcc
     5881 catgtgccat ccgcttccga tccagaaatg ttccgtagac tatgaactgg atgagacaaa
     5941 cggaattatc cgcgctttct gtacagtgaa aacagaaggc aagaccggtg tggaaatgga
     6001 ggcactgaca ggggttcagg tgacgcttct gacgatttat gatatgtgta aggcaattga
     6061 caagcatatg gtaatgtcca atattcatct ggttgaaaag acgggtggta agagtggaga
     6121 ttttcatttt tgatatccac gaattactct gcgatttgca aaatatttgt tgggtaaaat
     6181 gaaagaaatg tgtataatgg aaatacagaa tggcagataa cgaaaaaatt atctgcccga
     6241 cagggaggtt tttctaaaat gaaaagagta gcgatcatta catccagtga tacgggatat
     6301 cgcggagaac gtgaggattt aagcggacct gcgatccgag agattgttga gaagaatgga
     6361 tatgaggttg tgtctgaaga tgttctgccg gatgacagaa agatgttgtc agagagaatg
     6421 gcggaaattg cagacagcgg cacagcggaa ctgatcctga caacaggcgg aaccggtttt
     6481 tcacccagag atattacacc ggaggcaacg gaggatatta ttgacagacg tgtaccgggg
     6541 attccggagg caatgcgtgc gtacagcatg actatcacga aacgcgccat gctcagccgt
     6601 tctacggcag gtatccgaaa aaaaacactg atcatcaatc ttccgggaag tccgaaagct
     6661 gtaaaagaga gcctggagta tattattgat gcgcttggac atggcattga gatcatgaca
     6721 ggggaagcag ggaactgcgc aagataactg atggcgataa cagggataag aaagcagagt
     6781 agcgtcagaa tgtactgacg aaaagagaaa tcggtgagcc aaagcagaga agataagctg
     6841 acggcaataa acggaagagc aggtacgaaa acagtatctg aaacgaaaac aggaaggtaa
     6901 aagggacaat cttatgggaa aagtaattgc ggtttgtaca agtgaaagaa agggaataca
     6961 gaagacaagt gttccggaga ttaaagtaat tgaggactgg ggaatcgagg gtgatgccca
     7021 tgccggcaaa tggcaccgtc aggtaagtct gctctctttt gacaagattg aagattttcg
     7081 cgccagaggt gcagaggtag aagacggggc attcggagag aacctggtgg ttcagggaat
     7141 cgactttgcc acacttccga tcggtacgaa attccagtgc aatgatgtag tcctggagct
     7201 gacacagatt ggaaaagaat gccacagcgg atgtgcgatc tttaagaaaa tgggcgagtg
     7261 cattatgccg aaacagggag tttttacgaa ggttctccac ggaggtgtga tccacccggg
     7321 ggatgagctg gtaattttgt aacagatctt ttaaccggat tatagcaatc ctcataaata
     7381 atgtatattg atccggctta tcagagcgcg aaatcctgcg tcagatatcc ttgacgtgtg
     7441 ttgtttacag ggagtgctat aaattaagat tacatgagga gattggaaaa aatgattatt
     7501 gatacacatg cgcactatga tgacgaggcc tttgacacag acagggaagc gcttcttatg
     7561 tctatgtacg atggagggat tgagaagatt gtgaatgtct gtgcatctgt aggcggtttt
     7621 caggatactg tgaagctgat ggagaaatat ccttttgtat atggagcagt gggaatccat
     7681 ccggatgatg cagataagat gacacaggag actctggatg agattcgcag attatcccat
     7741 atggacaaga tggtggcaat cggtgagatc gggctggact attactggca taaggaagaa
     7801 gaggaacatc agatccagaa gaaaatgttt cgcgcccagc ttgacattgc cagagaagag
     7861 aagcttccgt ttatgatcca cagcagagaa gctgccgagg atactctgaa tatcgtcaga
     7921 gaatatatgc agggtggtat gtatggcggt atcattcatt gtttttcata cagcagggag
     7981 attgcggcag aatatctgaa aatgggactg tatctgggaa tcggcggagt tgtcactttt
     8041 aagaacgcaa agaaactgaa agagacggtt cagtatgcac ccctgtctca gcttgttctg
     8101 gaaacagact gtccctatat gtctccggag ccaaacagag gtaagagaaa ttcgtcatta
     8161 aatctgcctt atgtagcaca ggcaatcgca gaactgaagg gaattacagc agaagaggtc
     8221 attgatgtaa ccagacagaa tgcagagaaa ctgctgggag tttaaccgga tcaggtagcg
     8281 aagcggattg cgaatatctg cgtaattaaa aaagatgccg actaattaca gtcagcatct
     8341 tttttgtata caaatcggga attcatcaat aaccactgtc gtcggaagag tcttcggttc
     8401 cgtctgatgt gtctgcctga tattcccagg agtggatgga ttctgtatct tccactacat
     8461 ttccggtagt tttgtcgatc atgttgccct cccagtcaaa cttgacgttc agcaggtttg
     8521 tatttgtgga aggcttcaca ttcatggaca tgatttcctt aaatttagct ttcaggttat
     8581 cgagagtaaa ctcatcaccg atcacatttc ttacactgtg ggaagaagca agttcttctg
     8641 tataatcatc cagcatatat ggtccgtatt ctccgttatc gtgattataa tgcatgacca
     8701 taggtttcac ttccggagaa aggatctgga tggttgattt gccgttcaga gttgatttct
     8761 gtatggtaaa acttgccatt ccttccagaa gttccgggag ttcctgctgg gtggaaacga
     8821 agtttccgag ggagtagaat atggtggtat tgtgtccctg gtcatcagaa agctgtccgt
     8881 aaggctgaag cacatgcgga tgaccgccaa tgatcacatc aacaccctgc tgcatcagaa
     8941 aagcggccca ttgtttctcg tattcatttg gcatggtttc atcttctgtt ccccagtgag
     9001 ccacaaagat gatgcagtcg ctgatttctt tggctttctg gatcattgtg gtgatcttat
     9061 ccttatcaaa gatatcgatc atgtactcat atccttcgcc tgcaccggaa ttattggtgc
     9121 cgtatgtata gtcaagaaaa gcaatcttaa taccattcac ttcttttact ttgacagtgt
     9181 ctgcatcttc ctgggtggcg tggatgccaa gtacaggaat atccggataa ttggtgctcc
     9241 agaaatccag agtacttttc agatagtcat atccataatc atcggcgtga tttgtggcag
     9301 attccactac atcaaaccct gctttgataa tggcatctcc cacttcctgg ggagtagcaa
     9361 aagaaggata agtgctgaca gcatcgtggc ttggagcaaa aaccgtttcc tggtcgatca
     9421 tggctacgtc tgcagcctgg atctggtcca gaacatgagt gtagatatgg tcataattcc
     9481 atatgccgga gtcattttcg ccggattcat acagcttgct gtgataaaga ttatctccaa
     9541 cagcaattac acttgcagtg gagatgacag gtgtttcagc tttgatctta tcctgctgtt
     9601 ccttgagggc cgctgcttct gctgcttctt tctgcggttt acggatcagg gtgtaatcca
     9661 tctgatgcat caccagaacg agcagaacca gaacaataac agaacacaga agactgatcc
     9721 gggagttgat tctctcccgt aaattaagtg gcatattatt ttttttcata gaaaattccc
     9781 tttaactaaa atagttctta acagtgagag tgctgaacag ttacagcaat ctgcagcctg
     9841 tattgctgtc ctcagtaccg tatctcaaat tcattgcaga ctgcatacgg aaatgagaca
     9901 ggagcgtatg aactgtaatc tatctattca ttataacaaa tttcatggtt caaagaaaga
     9961 attaagagag aaattctttg aaaacctgcc agaaatgctt tgtgagacaa agtattttag
    10021 tgtataatag gtagcaatat aaccataatg tacaaacatg ctacagcaat gctatatgga
    10081 attgcttaag caaaaagagg gaggtaaccg gatgaaccat tatgaggcaa ttaatgatgt
    10141 actggtcaat ttatttaatg agattctgga cctggaagaa cgggcattga ttacgggaga
    10201 atataagaat atttccgtca atgatatgca cattattaat gcagtaggta tccgggaaca
    10261 gaagaatatg tcaacagtag ccagagaact gaatgtgact gtgggtacat tgacgatcgc
    10321 tgtgaataat cttgtaaaga agggatatat ccagcgtatg agaagtcagg aggaccgaag
    10381 ggttgtgctg atctccctga cagaacaggg gaagaaagct tattaccatc ataaagattt
    10441 ccatgagaag atggtactgg ctgtacttaa aggcctaaat gtggaggaga cagaggcact
    10501 gacgaaagct ctgaccaagt tgcagggatt tttcagatca tatcagtaga aggagtaaag
    10561 atggaatata ctgagaagga tttagtaaag attgcaaaaa gggaaaataa tacgaagaga
    10621 aactatctgg tggtggatcc gcttcaggga aagcatattc cggtagtgcc atcaaaggca
    10681 ctggatttat ttgctgctct ggcagatact ttcagggaga aatacaaaga tgaaaaactt
    10741 ttgctggtgg gctttgcgga gacagcaact gccataggtg ctcaggcagc gattactgtg
    10801 ggggcagatt atattcagac caccagagaa gttattcccg gcgtgaatta tctgtttttt
    10861 tccgaggaac acagccatgc cacagagcag aagctggtaa aagatgatat tgacagagca
    10921 gcggcggaga cggatcggat catatttatc gaggatgaag taactaccgg aaaaacaatt
    10981 cggaatatca tcagtattct tgacagggaa tatgacggaa aatttaaata ttcagtagca
    11041 tctctgttaa acggaatgag cgaggagaat ctggaacgtt ataagagaca ggggatttct
    11101 ctgtattatc tggtaaaaac ggatcacagt acttacggag acagagcaga aacatttaag
    11161 ggagacggat tttattataa atgtctggat aaggtcgtag agtatacaac gatttatgtt
    11221 aaaaacagga tggatgcccg cagactgatt gattccggga aatatgaaga agcatgtgag
    11281 aatctgtgga gagaaatccg ggagaaaacc ggaaacatgg ctgataatat atctggcaaa
    11341 agaatactgg tgatcggcac tgaggagttt atgttccctg cactttatat cggaa
//
LOCUS       DBMDXG010000017        11203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1649_length_11203_cov_2.219510, whole genome shotgun sequence.
ACCESSION   DBMDXG010000017 DBMDXG010000000
VERSION     DBMDXG010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1649_length_11203_cov_2.219510"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="ACZPD3_01185"
     CDS             complement(<1..188)
                     /locus_tag="ACZPD3_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414659.1"
                     /translation="MSRNIKGGFLTLSGVVGIVGMIIAAMQNPATAWVTPPGRMIVSI
                     LENGLLIPTVLFLVLFIYG"
     gene            complement(721..1122)
                     /gene="atpC"
                     /locus_tag="ACZPD3_01190"
     CDS             complement(721..1122)
                     /gene="atpC"
                     /locus_tag="ACZPD3_01190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1414660.1"
                     /translation="MSTFPLRIGTPDGLLFEGNVERVICRTVSGDLAILARHCNYCTA
                     LGMGEASVVMEDGSRRTAACIGGMLSVMNGKCRVLATTWEWKEDIDEQRAQKAKKRAE
                     EMIAKGGLSDHDYKIVEAKLQRALVRLSVKS"
     gene            complement(1138..2532)
                     /gene="atpD"
                     /locus_tag="ACZPD3_01195"
     CDS             complement(1138..2532)
                     /gene="atpD"
                     /locus_tag="ACZPD3_01195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1414661.1"
                     /translation="MSEKHIGEVVQVIGPVLDIRFAHDELPPLLNAIEIDNDGAKLVA
                     EVAQQVGDDVVRCIAMNSTDGLVRGAKALDTGGPISVPVGEECLGRVFNLLGDPVDNL
                     PAPEAKERWSIHRQAPSYEEQMPATEILETGIKVVDLICPYAKGGKIGLFGGAGVGKT
                     VLIMELIHNVATAHGGLSVFTGVGERTREGNDLYNEMKESGVIDKTALVYGQMNEPPG
                     ARMRVGLSGLTMAEYFRDEKNQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTL
                     ATEMGNLQERITSTRKGSITSVQAVYVPADDLTDPAPATTFSHLDATTVLSREISSQG
                     IYPAVDPLDSTSRILSPEIVGTEHYEIARSVQRVLQRYKELQDIIAIMGMDELSEEDK
                     RTVSRARKVQRFLSQSFSVAEQFTGMPGKYVPLKETLRGFRMILNGECDDIPESYFLF
                     VGTIDEVFEKAKNQ"
     gene            complement(2564..3421)
                     /gene="atpG"
                     /locus_tag="ACZPD3_01200"
     CDS             complement(2564..3421)
                     /gene="atpG"
                     /locus_tag="ACZPD3_01200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR1414662.1"
                     /translation="MASNMKAVKLRIKSVQSTMQITKAMELVASSKLRKAKERAEVCR
                     PYFTELHETLKEIARENTDFSSVYAKESSNNKTCYVVVAGDRGLAGGYNANLFKCLEA
                     SAEGKDYMVLPVGKKAVEYFRQREIEALTEQFAEVGSISVADCFEMANMLCTEFRKGT
                     FGHIELCYTVFNSMLSQQPEVFSMLPMTDIREESGGKIETKNLILYEPDGETVFDAIV
                     PEYLAGLVYGGICESTASELAARRMAMDAATSNAEEMIDQLNLYYNRARQASITQEIT
                     EIVAGAEGL"
     gene            complement(3442..4971)
                     /gene="atpA"
                     /locus_tag="ACZPD3_01205"
     CDS             complement(3442..4971)
                     /gene="atpA"
                     /locus_tag="ACZPD3_01205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1414663.1"
                     /translation="MQLKPEEISKVIRSQIKYYENAIQQNETGTILMVGDGIARASGL
                     VNCMSGELLEFEDGSFGMAQNLEENSVSIVIFGSDENIGEGQTVKRTGKVVSVPVGDA
                     MVGRVVNALGQPIDGAGPIDTKEFRPIESKAPGICERRSVYQPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTTIATDTIINQKGKDVICIYVAIGQKRSTVATLVENLTRNGAM
                     DYTIVVAATASESSPLQYIAPYSGCAMGEYFMNKGKDVLIIYDDLSKHAVAYRALSLL
                     IRRPPGREAYPGDVFYLHSRLLERAAKLDDEHGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLETELFHSGIMPAVNPGISVSRVGGDAQIKAMKKVAGTLKLIYSQYREL
                     QSFAQFGSDLDADTKARLEQGARIVEVLKQNQNAPVPVEKQVAILYAVTKGVLESVKV
                     EDVNVYESGLYTYLDTDAAGVEVMQEIRSTGKLEQETEQKLRSVLDAYTENFLNTRPE
                     K"
     gene            complement(5096..5635)
                     /gene="atpH"
                     /locus_tag="ACZPD3_01210"
     CDS             complement(5096..5635)
                     /gene="atpH"
                     /locus_tag="ACZPD3_01210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1414664.1"
                     /translation="MTKTARVYGGSLYDLAAEEKLDGQIMEQMIAVRQIFRENPGYLK
                     LLGEPAIPEDERLKMIEEAFGGQAERYLVNFLKLLCERKILREFAGCCEEFIRRYNSA
                     HGIAEAVVTSAVKLSDTQMEALKAKLEKLSGKKVYLVQKTDASVLGGLRVELEGVQLD
                     GTVQSRISGISKKLNELVV"
     gene            complement(5632..6135)
                     /gene="atpF"
                     /locus_tag="ACZPD3_01215"
     CDS             complement(5632..6135)
                     /gene="atpF"
                     /locus_tag="ACZPD3_01215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1414665.1"
                     /translation="MQVQELVGIVPWNFIATICNLFIQVYLIKRFLFKPINEMLEKRK
                     AKADAQIQDAVKAKEEAQAMKAEYEKNMQEAKNRANDIVMTAQKTAAIQSEEMLKEAS
                     SQVTAMKEKAEKDIAQEKRKAVNEIKGEIGGMAVEIAGKVIEREISEEDHAKLIDEFI
                     ENVGEAS"
     gene            complement(6254..6532)
                     /gene="atpE"
                     /locus_tag="ACZPD3_01220"
     CDS             complement(6254..6532)
                     /gene="atpE"
                     /locus_tag="ACZPD3_01220"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1414666.1"
                     /translation="MDFQLLAKGIALAGCAIGAGCALIAGIGPGIGEGNAVAKALEAI
                     GRQPECKGDVTSTMLLGCAIAETTGIYGFVTGLLLIFVAPGMFMNFLS"
     gene            complement(6663..7421)
                     /locus_tag="ACZPD3_01225"
     CDS             complement(6663..7421)
                     /locus_tag="ACZPD3_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1414667.1"
                     /translation="MELDISGARIFFTLPIDVPVLGPLRISETMVVSWIVMILITGLC
                     IWLTHDLKEENISKRQAVAELIVEKANSFVIGNMGEKFRYLIPFVAALFATSVVSNLI
                     SLIGLRSPTADLSTEAAWAVVVFIMITAQKIKTSGFGGYLKGFTTPIAVMTPFNILSE
                     LATPVSMACRHFGNILSGVVINGLIYGALAVASSALLGLVPGALGDVLSKIPILDVGV
                     PAITSVYFDWFSGVMQAFIFCMLTVMYIANAAEE"
     gene            complement(7478..7894)
                     /locus_tag="ACZPD3_01230"
     CDS             complement(7478..7894)
                     /locus_tag="ACZPD3_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414668.1"
                     /translation="MSIMDNVQPAVKKETGKVAAITGIGLILMWIVFGVLHAFMPQKV
                     PFDYTVFLGGAVGGCVAVLNFFFMGLAVQKAAAATDEDTARMRIKASYSQRMLIQMLW
                     VILAIVAPCFHFVAGIVPLLFPGTGIKIAGIFHTNK"
     gene            complement(7922..8191)
                     /locus_tag="ACZPD3_01235"
     CDS             complement(7922..8191)
                     /locus_tag="ACZPD3_01235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR1414669.1"
                     /translation="MKQWSEIIRNVTMLSQLGLSLITPVLICLAVCWLIVSKTGAGGW
                     VYIPGFFFGLGGSGTVAYKFYLSINRQQKKENKKKKNKVSFNRHL"
     gene            complement(8678..9787)
                     /locus_tag="ACZPD3_01240"
     CDS             complement(8678..9787)
                     /locus_tag="ACZPD3_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1414670.1"
                     /translation="MKRAAAAAISLMTGLAMLTGCSRAEVEETIQPLVADAIEESDSE
                     AEAVKEEDESPLIPEIDTDIKIHAGSRIAVVSKCVKGEYWKMVKKGMEDAVKEINKAY
                     GYKKDDQITMTFEGPDNEEDVETQINTIDAVIAENPDVLCISASDMDSCEAQLEAAKE
                     NGIPVIAFDSNVAEKKLVKAYRGTDNVQVGKMAAYQLGSALGKMGKVAVFSAQQKTKS
                     VQDRVEGFMNNIGKYGDIEVVETIYSDQVDDMEASMKEVLEKYPTLDGVFCTNADITE
                     MYLNLEKSDEHDAPALVGVDATTKQQEAIRNGEEIGCVSQQPYAMGYQTIWAAVETTA
                     PKKSVVIEKNVLSNPAWIDADCLDDPDYSGYLYTE"
     gene            complement(10220..10921)
                     /locus_tag="ACZPD3_01245"
     CDS             complement(10220..10921)
                     /locus_tag="ACZPD3_01245"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1414671.1"
                     /translation="MWIKDIRDYILYEDKDILVCHKPAGLAVQNARVGSMDMESLLKN
                     YIAQKVPGKMPYLGIIHRLDQPVEGVLVFALNPKAAADLSRQMTAGKIKKTYLAVTEG
                     TVKVKSAKLVDWLKKDGRTNSSAVVEGGTSGAKKAILSYEVLETWKNKEDAQDCGERN
                     LIRIDLDTGRHHQIRVQMAHAGMPLVGDRKYNPGQNSQEPLALCSAKLGFQHPVTKKR
                     LEFQVQPAGIAFKRH"
BASE COUNT         2897 a         2950 c         1932 g         3424 t
ORIGIN      
        1 ccataaataa aaagaacaag gaataaaacc gtaggaatca ataatccatt ttctaaaata
       61 cttacaatca ttcttccagg tggcgttacc catgcagttg ccggattttg cattgccgct
      121 attatcattc ctacaatacc aacaacacca ctcaatgtta aaaaaccacc ttttatattt
      181 ctactcataa tcattacctc ctcgttaaat accgatttgt tactcacatg atatcatatt
      241 ttttcttcaa ttacatccac ctaattttaa tacactgcca ttcggcttta ttcatcattt
      301 tttccttttg gtttccccgc aggggactta cctgattcgc ttaaagtgta tttaaaagag
      361 ccaaccacca gcatagaaaa ataatttatt tcatgtgcat ttgcaggata tttagatatt
      421 cttagctggt caaaatctgc gttatgagaa aaatgacaca ctgtctgagc gaagcgagtt
      481 tgtggcattt ttctcatgcc tttagcagat ttttagcagc ttagaatatc ttaatgtccg
      541 aaaccgcaca taaaataaat tatttttcta tgcgtccctc cttcaaatct cttcctcaga
      601 ttcttcccac tatatcctcc aaaaaaccat atcacaagca aaaaaagacc gacagtctcc
      661 tctcccggat ctcctgtcag tctttatctt taacacaata tcttaataac tcttattttc
      721 tcagctcttc acactcaaac gcaccaacgc cctctgcagt ttagcctcca caatcttata
      781 atcatgatca ctcaaaccac ctttggcaat catctcctca gctcttttct tagctttctg
      841 cgcacgctgc tcatcaatat cctctttcca ctcccaggta gttgcaagga cacggcactt
      901 tccgttcata acagaaagca ttccgccaat acaggctgcg gtccgtctgc tgccgtcttc
      961 cattaccaca ctggcttcgc ccattcccag agcagtacag taattacagt gtctggcaag
     1021 aatggccaga tcaccgctga cagttctgca gatcacacgc tccacattgc cttcaaagag
     1081 taatccgtcc ggtgttccga ttctcaacgg gaaagtactc ataggacatt tcccccttta
     1141 ctgattcttc gctttttcaa acacttcatc gatggttccc acaaacagga aataactctc
     1201 aggaatgtca tcacactcac cgttaaggat catcctgaat cctctcagcg tctccttcag
     1261 cggaacatat ttgccaggca tacctgtaaa ctgttctgct acagagaagc tctgtgacag
     1321 gaatctctga accttacggg cacggcttac agtacgtttg tcttcctctg acaactcatc
     1381 catacccatg atggcaatga tgtcctgaag ctctttgtag cgctgaagaa ctctctgtac
     1441 tgatctggca atctcataat gctctgttcc tacgatttca ggagaaagga tacggcttgt
     1501 ggaatccaga ggatcaacag ccggataaat accctggctg gaaatctcac gtgaaagtac
     1561 agttgttgca tccagatgtg agaatgtagt tgcaggagcc gggtcagtca ggtcatcggc
     1621 aggcacatat acagcctgta cagatgtgat ggaacccttt cttgtggaag taattcgttc
     1681 ctgcaggtta cccatttcag ttgccagtgt aggctgataa cctacggctg aaggcatacg
     1741 tcccagaaga gcagacacct cggaacctgc ctgtgtaaaa cggaaaatat tgtcaataaa
     1801 gaggagcaca tcctggttct tctcatcacg gaagtattcc gccatggtaa gtccggaaag
     1861 tccaactctc atacgtgctc cgggcggctc gttcatctga ccgtaaacca gagcagtctt
     1921 gtctataact ccactttctt tcatttcatt gtaaaggtca tttccctcac gtgtacgctc
     1981 tcctacacct gtaaatacgg aaagtccgcc gtgtgcagtt gctacgttgt ggatcagctc
     2041 catgataaga actgtttttc ctacacctgc accaccgaaa agaccgatct taccaccctt
     2101 tgcataagga cagatcaggt caacgacctt aattcctgtc tccaggatct ctgttgcagg
     2161 catctgttct tcataagaag gggcctgacg atggatagac cagcgttcct ttgcctccgg
     2221 tgccggaaga ttgtctaccg ggtcacccag aaggttaaat actcgtccca ggcattcctc
     2281 acctacagga acagaaatgg gaccgcctgt atccagagct ttggcaccgc gcaccagacc
     2341 gtcagtagag ttcatggcaa tacatcttac cacatcatca ccaacctgct gtgcaacctc
     2401 ggcaaccagc tttgcgccat cattatcaat ctctatggca ttgagaagag gaggcagttc
     2461 atcgtgcgca aagcggatat ccagtaccgg accgataacc tgcactacct cgccaatgtg
     2521 tttttcactc atgttagtaa tctcctatct gttggaaatt atattaaaga ccttctgcac
     2581 ctgcaacaat ctccgtgatc tcctgcgtga tgctggcctg acgtgccctg ttgtaataca
     2641 gattgagctg atcgatcatc tcttccgcat tacttgtggc tgcatccata gccatacgtc
     2701 tggctgccag ttcacttgca gtactttcgc agataccgcc ataaaccagg cctgccagat
     2761 attccggaac aatggcatca aatactgtct ctccgtccgg ttcatagagg atcagattct
     2821 tcgtctctat cttacctccg ctttcctctc tgatatcagt cataggaagc atggaaaata
     2881 cctccggctg ctgagaaagc atggaattaa agacagtata acataattca atatggccaa
     2941 aggttccttt tcgaaattcc gtgcagagca tatttgccat ctcaaaacag tccgccacag
     3001 aaatacttcc cacttctgca aactgttctg tcagagcttc aatctctctc tggcggaaat
     3061 attctactgc tttctttcca accggcagaa ccatgtagtc cttaccttca gcagaagcct
     3121 ccagacattt gaaaagattt gcattatatc cgcctgccag tcctctgtca cctgcgacca
     3181 ctacatagca ggttttattg ttggaagact ctttcgcata aacggatgag aaatctgtat
     3241 tctcccttgc aatctctttc aatgtctcat gaagttctgt aaagtacgga cggcatacct
     3301 ctgcacgttc ctttgcctta cgcagtttgg aggatgctac aagctccatg gccttggtaa
     3361 tctgcattgt actctgtaca ctcttgatac gcagctttac cgctttcata ttcgaagcca
     3421 tacgataccc tcctaatcac tttatttctc aggtcttgta ttcaggaagt tctctgtata
     3481 cgcatccagt acgctgcgaa gcttctgttc tgtttcctgc tccagcttac ctgttgagcg
     3541 gatctcctgc ataacctcta cgcctgctgc atctgtatcc agataggtat ataatcctga
     3601 ttcatataca tttacgtctt caacttttac tgattccaga acaccctttg taaccgcata
     3661 aaggattgca acctgtttct ctacaggaac cggtgcattc tggttctgtt tcagaacttc
     3721 cacaatacgt gctccctgtt ccagacgcgc ttttgtatca gcatccagat cagaaccaaa
     3781 ctgtgcgaag ctctgcagct cacggtactg ggaataaatc agtttcagtg ttcctgctac
     3841 cttcttcatt gccttgatct gcgcatcacc accaactcgt gatacggaga tacccggatt
     3901 tacagcaggc atgattcctg aatggaataa ctctgtctcc aggaatatct gtccgtctgt
     3961 aatagaaata acgttagtag gaatataggc agatacgtca cctgcctgtg tttcaatgat
     4021 cggcagtgct gtcatggaac cgccgccatg ttcatcgtcc agcttcgctg cacgttccag
     4081 aagtctggaa tgcagataga aaacatcacc cggatatgcc tcacgtccag gcggacgacg
     4141 gatcagcagg gaaagtgcac ggtatgcaac tgcatgctta cttaaatcat catagatgat
     4201 cagcacatct tttcccttat tcataaagta ttcacccatt gcacatccgg aatacggtgc
     4261 gatatactgc agcgggcttg actcagatgc tgtagctgcc acaacgatag tataatccat
     4321 tgcgccgttt ctggtcaggt tctctactaa tgtagctact gtggaacgct tctgtccaat
     4381 ggctacatag atacagatca catcttttcc cttctggtta ataatggtat ctgtagcaat
     4441 tgtagtttta ccggtctgac ggtcaccaat gatcagttct cgctgtccac gtccgatagg
     4501 gatcatagag tcgatggctt taataccggt ctgaagaggc tgatatacag atcttctctc
     4561 acagattcct ggtgccttgc tctcgattgg ccggaattct tttgtatcaa ttgggcctgc
     4621 tccgtcaatt ggctgtccca gtgcatttac aacacgccca accatggcgt cgcctacagg
     4681 tacagacacg accttgcctg tacgctttac ggtctgaccc tcaccaatgt tctcatctga
     4741 accaaagatt acgatggaga cactgttctc ctccaggttc tgtgccatac caaagcttcc
     4801 gtcctcaaat tccagaagct ctcctgacat acagtttacc agtccgctgg ctctggcaat
     4861 accgtcacca accataagga tcgtacctgt ctcgttctgt tgaatggcat tttcgtaata
     4921 ttttatctgg cttcggatga ccttggatat ttcctcaggt tttaattgca tgtttccatt
     4981 ccatcctttt atattctatt attttgcgct gtctgaattc agaaatttaa agatttttca
     5041 tatccatatg tcaaataccc atacagtacg attctcaaat ccggaatcca gttccttata
     5101 ctaccaattc attcagtttc ttcgaaatac cggaaatacg gctctgtact gtaccgtcaa
     5161 gctgtacgcc ctccagttcc acgcgaagtc cgccaagaac ggatgcatct gttttctgaa
     5221 ccagatacac tttctttcca cttagcttct caagcttcgc tttcagagct tccatctgag
     5281 tatcactgag cttcactgca ctggtaacca cggcttctgc aattccatgt gcactgttgt
     5341 agcgtctgat aaactcttcg cagcatccgg caaactctct cagaatcttg cgttcacaca
     5401 ataatttcag gaaattcacc agatatctct ctgcctgccc tccgaaagcc tcttcaatca
     5461 tcttaagtct ctcgtcttca ggaatcgccg gttctcccag caatttcaaa tatccgggat
     5521 tttctcgaaa aatctgtctg actgcgatca tctgctccat gatctgtcca tcgagctttt
     5581 cttcagcggc aaggtcatac agacttccgc cgtaaactct ggcagttttt gtcatgatgc
     5641 ctcacctaca ttctcaataa actcatcaat cagttttgca tgatcctctt cactaatttc
     5701 gcgctcgatc acttttccgg caatctcaac agccataccg ccgatttctc ccttaatctc
     5761 gttgactgcc ttacgtttct cctgggcaat gtctttctct gccttctctt tcatggcagt
     5821 tacctgactg gaagcctcct taagcatctc ttcactctgg atagcggctg ttttctgagc
     5881 tgtcatcaca atgtcatttg ccctattctt ggcttcctgc atattctttt catattctgc
     5941 cttcattgcc tgagcttctt cctttgcctt cactgcatcc tggatctggg catccgcttt
     6001 cgctttgcgc ttttccagca tctcattgat gggcttgaag aggaaacgtt tgatcagata
     6061 tacctgaata aaaaggttgc aaatcgtcgc tataaaattc cacggcacaa ttccaactaa
     6121 ttcctgtacc tgcatgtttg taacctcctg tctgtattta tgcggatcat tctccggctg
     6181 ttgccggaat tatccacagg ttccatatca gatgcctctt aatctgtgat attcgacatc
     6241 tcagctgttt ttatcagctc aggaagttca tgaacatacc aggtgcaacg aagataagca
     6301 acagaccggt tacgaaaccg tagatacctg ttgtctctgc aatggcacat ccaagaagca
     6361 tggtagaagt aacatcaccc ttgcactcag gctggcgtcc gattgcctcc agcgcctttg
     6421 ctactgcgtt accttcacca ataccaggtc cgatacctgc gatcagcgca catcctgctc
     6481 caattgcaca tcctgctaat gcgatacctt ttgccaataa ctgaaaatcc atattaaaac
     6541 cctcctgtta aataataatc tgcagctttt cccctttgct gcggtattaa tttttctgct
     6601 gtatttcagc attctttgcg gttaccaccg ctgccgtttt gctccggcaa atcatgtttc
     6661 tgctactctt ctgctgcatt tgcaatatac attacagtca gcatacagaa gataaacgcc
     6721 tgcataactc ctgagaacca gtcaaaatag accgacgtta tcgcaggtac accgacatca
     6781 agaatcggta tctttgacag tacatctccc aatgcacccg ggaccaggcc cagaagcgcg
     6841 gaacttgcga ctgccagtgc cccatagatc aggccattga ttaccacacc ggaaaggata
     6901 tttccgaaat gacgacacgc catactgaca ggtgtagcca gctcggaaag aatattaaac
     6961 ggtgtcataa ctgcgatcgg agttgtaaat cctttcagat aaccgccaaa tccgctggtc
     7021 tttatcttct gtgctgtgat cataatgaaa accacaactg cccaggcagc ctcagtagaa
     7081 aggtctgctg tagggcttcg aagtccgatc agactgatca ggttggaaac cacactggtt
     7141 gcaaaaagtg ctgcaacaaa tggtatcagg tatctgaatt tctcacccat atttccgatt
     7201 acaaagctgt tggccttctc tacaatgagc tctgccacag cctgacgttt ggaaatgttc
     7261 tcttctttca gatcatgtgt cagccagata cacaaacctg ttatgagtat cataacaatc
     7321 cagcttacca ccattgtctc actgatcctt aatggtccga gaacaggcac atcaatgggt
     7381 aatgtaaaga atatccttgc cccgctgata tccaattcca tactctttat tcacctcctg
     7441 tctctcccgg tactgttccg gtttgatata gaaataccta tttgttagta tgaaaaattc
     7501 ctgcaatttt aattcctgtt ccgggaaata aaagaggaac gatacctgcc acaaaatgaa
     7561 aacagggtgc cacgattgcc aggatcaccc acaacatctg gatcagcatt ctctgggagt
     7621 aacttgcttt gattctcatt ctcgctgtgt cctcatctgt tgctgcagct gctttctgca
     7681 ctgcaagtcc cataaaaaag aaattaagga ctgccacaca tcctcccact gcccctccaa
     7741 gaaacaccgt ataatcaaac ggtaccttct gtggcatgaa agcatgcagc actccaaaca
     7801 cgatccacat caaaatcagc ccgattccgg tgatggcagc taccttcccg gtttccttct
     7861 ttacggcagg ctgaacatta tccataatac tcatataaat ctccctttcg tttctgtatc
     7921 attacaggtg tctgttaaac gatactttat ttttcttctt tttgttctct ttcttctgct
     7981 gtctgttaat agaaagataa aatttatatg ccactgtccc ggaacctcca agaccaaaga
     8041 aaaaaccggg gatatatacc catccgccag ctccggtctt agaaacaatc agccagcata
     8101 ctgccagaca tatcaggaca ggggttatga gagacagtcc aagctgcgac aacatggtga
     8161 catttctgat gatctctgac cattgtttca tcctcttctc ctttttgcct aattttttta
     8221 tatattttca ataatttata gtcaaatttt atcatatatc ctttaaaatt acaatctcat
     8281 taaataattt tatcaaatat tttttatcta tccaatattt ttttgttatt tttatatcgt
     8341 gtatgtgatt atatcatcaa tataaatctt tttatacagt caaagcttct gattggttta
     8401 tatttttatt tcaaagaaga agagatataa ataaggagcc tgcccggaga gcttttttgt
     8461 gtcatagaac attacggagt aatattctat gacacaaaaa aagcacgaaa acaactcatc
     8521 actgttttca tgcttcttct ttcgtatata attctgcttt atatataagt tctgtattac
     8581 cggtctgtcc cagcagacac attcctctta tagaaaatcc tcttaattaa tgcatttgag
     8641 accctgtaca tcctcccgga gcatttatct ttaattctta ttctgtatat aaatatccac
     8701 tgtagtccgg atcatccaga cagtctgcat cgatccatgc cggattgctc agtacatttt
     8761 tctcgattac aacagatttc ttcggtgccg ttgtttcaac tgctgcccag atcgtctggt
     8821 atcccatagc ataaggctgc tgagaaacac agccaatctc ttctccatta cggattgctt
     8881 cctgctgctt agtagttgca tcaaccccta ccagagccgg tgcatcatgc tcatcactct
     8941 tctccagatt cagatacatc tctgtaatat ctgcattggt gcagaataca ccatccagtg
     9001 tgggatattt ttccagaact tccttcatgg aagcctccat atcatctacc tgatcagaat
     9061 aaatagtttc cacaacttca atgtctccgt acttcccgat attattcatg aatccttcta
     9121 cacgatcctg aacactcttt gtcttctgct gtgcggaaaa aaccgcaacc tttcccatct
     9181 tcccgagtgc actgccaagc tgataagcag ccatctttcc tacctgcaca ttatcggttc
     9241 cccgatatgc cttcaccagt tttttctcgg ctacattaga atcaaatgct ataaccggaa
     9301 ttccattctc ctttgcagct tcaagctgtg cctcacagga atccatgtca cttgccgaaa
     9361 tacaaagaac atccggattc tctgcgataa cagcatctat tgtattgatc tgagtctcta
     9421 catcctcttc attatcagga ccttcaaacg tcatggtaat ctggtcatct tttttataac
     9481 catatgcttt attaatttcc tttaccgcat cttccatgcc tttcttaacc atcttccagt
     9541 attcgccctt gacacatttg cttaccactg cgatccttga accggcatgg atttttatat
     9601 ctgtatcaat ctccggaatc agcggactct catcctcttc cttcactgct tctgcctctg
     9661 aatcggattc ttcgatagcg tctgccacca acggctggat tgtctcttcc acctccgctc
     9721 tgctgcatcc tgtcagcatt gccagtcctg tcattaaact tatcgctgct gccgccgctc
     9781 ttttcatttt aatttcctct tcctttcagt gctatactgc acattacagc aatttcataa
     9841 aataatgtat aatttctgtc aactaaaaaa tcgatatctg ctcagtactc tcacagaata
     9901 attaacatcg aacaattacc tgtagcgatc ctcttaaatg cattatttac gagaattgct
     9961 ataaacctta taactgcata ttactttcta cattatacac actttacgaa attcgtccag
    10021 agattttaca catttttcac ttttatacat caaaaattgt tactgttcac tccattctca
    10081 gcaacacgct tcgcgatgca cagaatttat gctaatcgca taaattcgcg cggtatgtct
    10141 cgggttttct gtgacctttg ctcacaaaaa acacctcgcg ggatatcacc agtgaacagt
    10201 aaccaaaaat tgcgctatat caatgtcttt tgaatgcaat acctgcaggc tgcacctgaa
    10261 actccaaccg tttctttgtc accggatgct gaaatcccag ttttgcagaa cagagagcca
    10321 gcggttcctg actattctga ccgggattat actttctgtc accaaccaaa ggcattccgg
    10381 catgcgccat ctgcacacga atctgatgat gcctgcctgt atccagatca atccgaatca
    10441 gatttctttc gccacaatcc tgtgcatctt ctttattttt ccaggtttcc agaacttcat
    10501 aagacaatat cgcctttttt gctccggaag tccctccctc aaccactgca gagctgttgg
    10561 tcctgccatc cttcttcagc cagtccacca gttttgcact cttcactttc actgttcctt
    10621 ctgtaactgc aagataagtc ttctttattt ttcccgctgt catctgtctg ctgagatctg
    10681 ctgctgcctt cggattcagg gcaaacacaa gaaccccctc caccggctga tccaggcgat
    10741 ggataatacc cagatacggc atcttacccg gcaccttctg agcaatataa ttcttcaaga
    10801 ggctctccat atccatactg ccaacccgcg cattctgaac tgcaagccct gccggtttgt
    10861 gacagaccag aatatcttta tcttcatata aaatataatc tctgatatct tttatccaca
    10921 taaaaaactc tccttttcat ggagagtata tcctaaaatt ccatttcacg caataaacga
    10981 atatatatga atacaatttg aaaacggtca tcacttttta cttttcctga tttcaaaaat
    11041 gatgaccgct tcaatgttct tcgttattca atttattttc ctcttagtat tctttctata
    11101 ataaaataat atgttattta caaactactt tataagttca ctattaaatt tttctatgtt
    11161 tatttaaaat tcctattctg tttcctgctc ataatccata tgt
//
LOCUS       DBMDXG010000018        11079 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1665_length_11079_cov_1.628621, whole genome shotgun sequence.
ACCESSION   DBMDXG010000018 DBMDXG010000000
VERSION     DBMDXG010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11079)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_11079_cov_1.628621"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="ACZPD3_01250"
     CDS             <1..1224
                     /locus_tag="ACZPD3_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pullulanase PulA and
                     related glycosidase"
                     /protein_id="HIR1414672.1"
                     /translation="APKRSYCATDDPEKEFKTFIKKLHQAGIACIMEMYFPRECNPVT
                     ALRALQFWKLYYHVDGFHVLGEGVSAKLLMHDGVLSDTRLMFHDFDESQIRKKKKPED
                     KCIAQYNPGFLQDMRRFLKSDEDMVSAAAYHIRRNPNTYAVINYMACQDGFTMNDMVT
                     YNYRHNEANQENNHDGSSYNYSWNCGVEGPSRRLQIRQMRERQIRNAFLMVLLSQGVP
                     MIYGGDEFGNSQNGNNNAYCQDNQVGWIDWKALKKNESLFQFVKNAIAFRKEHPILHV
                     PGEMYGVDYQTRGLPDVSLHGERAWYMNSENTSRLLGIMYCGAYAHRADGSEDASIYV
                     AYNFHWEDRIFALPNLAGYRKWKKVIDTSAVKENGFLEQEQETYSKKLKVTPRTIVVL
                     MAVEEEKKDASVAAL"
     gene            1202..1756
                     /locus_tag="ACZPD3_01255"
     CDS             1202..1756
                     /locus_tag="ACZPD3_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR1414673.1"
                     /translation="MHPWLHFKTITRHKLLVMHYCFRAGMYKQGLLHDLSKYAPVEFL
                     VGCKYYQGDRSPNNAEREDTGISKSWLHHKGRNKHHFEYWVDYAPGDEHIINGVPMPR
                     KYIAEMVMDRISASRNYLGDKYDQHQPLDYYLKGKEKLWFIHPKTKRDLEGLLRILND
                     HGEEVLISYIKNVYLKKDKALERV"
     gene            complement(1720..1836)
                     /locus_tag="ACZPD3_01260"
                     /pseudo
     CDS             complement(1720..1836)
                     /locus_tag="ACZPD3_01260"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            1795..1884
                     /locus_tag="ACZPD3_01265"
     CDS             1795..1884
                     /locus_tag="ACZPD3_01265"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1414674.1"
                     /translation="MFCPNSVVVAHYDALIRTKSPTNCDAHLA"
     gene            2272..3807
                     /gene="putP"
                     /locus_tag="ACZPD3_01270"
     CDS             2272..3807
                     /gene="putP"
                     /locus_tag="ACZPD3_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HIR1414675.1"
                     /translation="MSGTTMVILSAFVAYLLLMIVIGVVYMKKTSSSEDYFLGGRGLN
                     AWVAALSAQASDMSGWLLMGLPGAIYSLGTGQIWIAVGLFIGTVLNWVCISHRLRKYT
                     IAANNSLTIPAFLENRFQDKKRILLLLSSIVIVIFFLVYTASALAAGGKLFNTVFGID
                     YHIALAIGAAVILCYTFMGGFMAVCVTDFVQGTLMLIGLLIVPLVAYFTLSGSLSDLL
                     TQSGAPGGAAAFLNPFENGERPYTFVEIFSQLAWGLGYCGMPHILTRFMAVKSEKELK
                     KSSAIAIVWDILSLTAACFIGIIGRAYLLPTVLGENGASSSESVFIEMINKLFSSHLG
                     LPFIGGIFLCGILAAIMSTADSQLLVTASAASEDLYHQFIKKDADSKEILAVARLTVI
                     VVSVLAFVIAWNPNSSIMGLVSNAWAGLGAAFGPTVVMSLFWRRTNLAGAVAGIVSGG
                     LTVIVWDYIPFVAGQTLGSYTGLYSLAVGFAVSLAMIIIFSLATKAPSKEITDVFDKV
                     TGK"
     gene            3981..5096
                     /gene="glf"
                     /locus_tag="ACZPD3_01275"
     CDS             3981..5096
                     /gene="glf"
                     /locus_tag="ACZPD3_01275"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR1414676.1"
                     /translation="MKKYDYLIVGAGLFGAVFAHEATRRGKTCLVIDKRGHIGGNIYT
                     EEVEGIQVHRYAAHIFHTSRKQVWDYINQFAEFNHFVNSPIAVYKDELYNLPFNMNTF
                     HQLWGVRTPAEAKAKIQEQIARMHITHPKNLEEQALALVGQDVYEKLVEGYTRKQWGR
                     ECKDLPAFIIKRLPLRYTYDNNYFKDPYQGIPKGGYTRIVKKLLEGVQVCLNTDFFAN
                     REELTAQADKVLFTGMIDEFYDYCYGELEYRSLRFETEVLDMGNYQGNAVVNYTDYEV
                     PYTRIIEHKHFEFGTQPKTVITREYPAAWEKGKEPYYPVNDPKNSELFDKYERRALEE
                     KNVIFGGRLGMYRYMDMDQVIEEALSLAETELTYEEP"
     gene            5180..5764
                     /locus_tag="ACZPD3_01280"
     CDS             5180..5764
                     /locus_tag="ACZPD3_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6553 family protein"
                     /protein_id="HIR1414677.1"
                     /translation="MTDTPTIALYYREHDPMKRKMFLDQSIAAGEDEEANAVRKELWE
                     LRYGEPSEAGSGTRADGYLALWMAMEYSKDTAGKLFGLKRARKEIEKNLARLKFREMQ
                     EKSELHRELLYRECCHMVKTYMELCEKDKAYNTILCGIVHISEKSAKSKIQNDIYTTA
                     IAFPEEINMQEELEMITRAAREVYEAHFPGEGGL"
     gene            5798..8722
                     /locus_tag="ACZPD3_01285"
     CDS             5798..8722
                     /locus_tag="ACZPD3_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR1414678.1"
                     /translation="MNKENLTAYEVVTEENLTDIHSTGWLLRHKKTGARVMLIENDDE
                     NKVFNIAFRTPPKDSTGVAHILEHSVLCGSREFPLKDPFVELVKGSLNTFLNAMTYPD
                     KTCYPVASCNDKDFQNLMHVYLDAVFYPNIYKREEIFRQEGWNYHLEQKEGPLKYNGV
                     VYNEMKGAFSSPDEVLEREIMNHLFPDTTYGCESGGDPKNIPDLTYENFLNFHRTYYH
                     PSNSYIYLYGNMDMEEKLAFLDEHYLSHFDYLDVDSVIQEQKAFGACQDVTLEYPVAE
                     NEGEEDNTYLSYNMVVGNAADSQMAMAFEVLDYALLSAPGAPLKQALLDVKAGKDVYG
                     SYDDGILQPYFTVIAKGSNPDRKEEFVSVIRQVLGDIVKNGIDRKAVEAGINYFEFRY
                     READFSSYPKGLMYSLDILGDWLYEKGNPFAQVQQLTVFEKLKKAVNEGYFEELIRKY
                     LLENPHGCIMTLVPKKGLAAQREKELEEKLEAYRSSLSEEQLDAMVEKTKALEAYQEA
                     GEDPKALECIPMLKRSDIKREAAKIINEELTVDDSLFLYHDVCTNGIGYVDLMFKTDS
                     IAPEQIPYLGLLKSVLGYVDTENYTYGELFNEINANTGGINCGVEVFDRADSTEEFQA
                     MFSVRGKALYTKMDFLFKMIGEILNSSKLEDTKRLYEIVASVKSRAQVNLTGAGHSTA
                     VLRAAAYSSPMAAFQDEMAGIGYYQFIEKLEKDFEQRKEETVEELCKLMKKILRPENF
                     MISYTGERESLETVQKLAGAVKAGLGTEPVEKSEEKLTCTKKNEGFKTSGQVQYVAQT
                     GNFKKKGLEYTGALEILKVILSYDYLWMNLRVKGGAYGCMSGFKRNGESYLVSYRDPH
                     LKRTLDVYKGIPDYIRNFQADEREMTKYIIGTISGKDVPRTPQMKGSVSKTAYFCGVT
                     EEMLQKERDQILNASAEDIHALAEIIEAVLAADQICVVGSESKVAEASDVLMEVKPLI
                     NC"
     gene            8739..9647
                     /gene="era"
                     /locus_tag="ACZPD3_01290"
     CDS             8739..9647
                     /gene="era"
                     /locus_tag="ACZPD3_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR1414679.1"
                     /translation="MNENFKSGFVAIIGRPNVGKSTLMNHLIGQKIAITSKKPQTTRN
                     RIQTVYTCDEGQIVFLDTPGIHKAKNKLGEYMVQVAERTLKEVDAIMWLVEPSTFIGA
                     GERHIAEQLQGIGVPVILIINKIDTVSKEEILPAIDTYRKVCDFAEIIPCSALRGQNT
                     QDIIGCILKYLPYGPMFYDEDTVTDQPQRQIVAEIIREKALHALDAEIPHGIAVAIDR
                     MKTRPGKNPIVDIDATIICERESHKGIIIGKQGAMLKKIGSNARYEIERMLESKVNLK
                     LWVKVKKDWRDSDFLIKNFGYDKKEI"
     gene            9676..10428
                     /gene="recO"
                     /locus_tag="ACZPD3_01295"
     CDS             9676..10428
                     /gene="recO"
                     /locus_tag="ACZPD3_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR1414680.1"
                     /translation="MSDLITVQGVVLSAMPVGEYDKRIVLLTRERGKISAFAKGARRP
                     NSQFLAAANPFVFGTFALYEGRSSYNLNQVSISHHFVELAGEQPGIYYGYYFLELADY
                     FGQEGIDEKESMNLLYVTVKALLNPNIDNRLVRCIFELRMMAAQGLCPSLFHCVCCER
                     QPVEGEELFFSQQNHGILDKACLGHVNDAKRISAPALYAMQYIVTASLGKLYTFTVTE
                     EVLHELERHIHTYIAANTEKRFKSLEILEIMS"
     gene            10581..>11079
                     /locus_tag="ACZPD3_01300"
     CDS             10581..>11079
                     /locus_tag="ACZPD3_01300"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742709.1"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR1414681.1"
                     /translation="MEKTMDKIIALAKARGFVYPGSEIYGGLANTWDYGNLGVELKNN
                     VKRAWWQKFIQESPYNVGVDCAILMNPQTWVASGHLGSFSDPLMDCKECHERFRADKI
                     IEDFSQENGIELESSVDGWSQEEMMNFIKDHNVPCPTCGKHNFTDIRQFNLMFKTFQG
                     VTEDAK"
BASE COUNT         3423 a         1998 c         2780 g         2878 t
ORIGIN      
        1 gcacccaaga gatcttactg tgcaacagat gatccggaga aagagtttaa aacattcatt
       61 aaaaaactgc atcaggccgg aattgcctgt atcatggaaa tgtattttcc acgagaatgt
      121 aatcctgtga cagcactcag ggcattgcag ttctggaagc tttattatca tgtagacgga
      181 tttcatgtat tgggtgaggg agtttctgcc aaattgttga tgcatgacgg agttttaagt
      241 gatacccggc tgatgtttca cgattttgat gagagccaga tccggaaaaa gaagaaaccg
      301 gaagacaaat gtattgcaca gtataatccg ggatttttgc aggatatgcg ccgttttctg
      361 aaaagtgacg aggatatggt ttcagcggct gcttaccata tcagaaggaa tccaaatacc
      421 tatgcagtca ttaattatat ggcctgtcag gatggcttta ccatgaatga tatggtgacc
      481 tataattaca gacataatga agcaaatcag gagaataatc acgatggcag cagttataat
      541 tattcctgga actgtggcgt ggaagggcca agcagaaggc tgcagatcag acagatgaga
      601 gaacgtcaga tcagaaatgc ttttcttatg gtgctactca gtcagggtgt tcctatgatc
      661 tatggcgggg atgagtttgg aaattcgcag aacggaaata ataatgccta ttgtcaggat
      721 aatcaggttg gatggattga ctggaaagca ctgaagaaaa atgaatccct gtttcagttt
      781 gtaaagaatg ccattgcttt ccggaaagaa catccgattc ttcatgttcc gggagaaatg
      841 tacggagtgg attatcagac caggggactg ccggatgttt cccttcatgg agaacgcgca
      901 tggtatatga attctgagaa cacatccaga ctgcttggaa ttatgtattg tggcgcctat
      961 gcgcaccggg cagacggcag cgaggatgct tccatatacg ttgcatataa ctttcactgg
     1021 gaagatcgta tttttgcgct tcctaatctg gcaggataca gaaaatggaa gaaagtgatt
     1081 gataccagtg ctgtgaaaga gaatggtttc ctggagcagg aacaggaaac ttatagtaaa
     1141 aagctgaagg tcacaccgcg tacgatcgtg gtgctgatgg ctgtagagga ggagaagaaa
     1201 gatgcatccg tggctgcact ttaaaacaat aaccagacat aagctgttgg ttatgcatta
     1261 ctgtttccgg gctggtatgt ataaacaggg actgcttcat gacctgtcca aatatgcacc
     1321 tgtggaattt ctggttggat gtaaatacta tcagggagac agaagtccca acaatgcaga
     1381 aagagaagat acaggaatat caaaatcctg gcttcatcat aaagggcgca acaaacatca
     1441 ttttgagtac tgggtagatt atgcaccggg agatgagcat atcatcaatg gtgtgcccat
     1501 gccccgtaaa tatattgcgg aaatggtgat ggacaggatc agtgcttcca gaaattatct
     1561 gggggataag tatgatcagc atcagccgct ggactattat ctgaaaggta aggaaaagct
     1621 gtggtttatc catccgaaga caaagagaga tctggagggg ctgctgcgga tacttaatga
     1681 tcatggggaa gaggtactga tttcctatat taagaatgtt tatctgaaaa aagataaagc
     1741 cctggagcgt gtctgaaaaa tcattcccgc aatctgcacg ccccactttg cggtatattt
     1801 tgtccgaatt cggttgtcgt agcccactac gacgccctca tccgaacaaa atctcccaca
     1861 aattgtgacg cacatcttgc gtaaagcatt tttcagacac gctctggcag gcagaaaaca
     1921 tacagcaaaa agagaatgta aacaggctgg cagacagaaa cagataaatt tctgattgac
     1981 agagtacctg tttgggtgta aaaatataaa acagttactg tgaaggtctt tgccggacgg
     2041 taacagtaat aattccataa aatggcggaa aaatgtcgaa aaacaagaaa tacacttgcg
     2101 tgatgctaag gtaatgtggt aaagtagccg ggtacagaag tggtacccgg ttactttttg
     2161 cagtatataa aatgactgta ttctgttttt ggataaatat gtgaaggaaa cagaacacac
     2221 tttgacaaat ccagagtgta tgaaaaaaag aagaaaaaag gaggagtaaa tttgagtggt
     2281 acaacaatgg tcatattgtc cgcatttgtt gcctatctgt tgcttatgat agtcattggt
     2341 gtggtttata tgaaaaaaac aagcagctcg gaagattatt ttctgggcgg acgtggactg
     2401 aatgcatggg tagctgctct ttcagcacag gcatcagata tgagcggatg gctcttaatg
     2461 ggacttccgg gagcaattta ttcactggga acaggacaga tctggattgc tgtaggtctg
     2521 ttcatcggaa ctgtattaaa ctgggtgtgt atttcacaca gacttcgtaa atatacgatc
     2581 gcagcaaaca attcccttac aatcccggca tttcttgaga acagatttca ggataagaag
     2641 agaatcctgt tattgctttc atccatcgtg atcgtaatct ttttcctggt ttatacagct
     2701 tctgcactgg cagcaggcgg taaacttttt aacacagtat ttggaattga ttatcatata
     2761 gctctggcaa ttggtgcagc agttatcctc tgttatacat ttatgggggg atttatggct
     2821 gtatgcgtaa ctgactttgt acagggaaca ctgatgctga tcggtcttct gatcgttccg
     2881 ctggttgcct actttacact gagcggaagt ctgagtgatc ttctgacaca gagcggagca
     2941 ccggggggag cagcagcttt cctgaatcct tttgagaacg gagagcgccc atatacattt
     3001 gttgaaatct tttcccagct tgcatgggga cttggatact gcggtatgcc tcatatcctt
     3061 acccgcttca tggctgtaaa gagtgagaaa gaattaaaaa aatccagtgc gatcgcaatc
     3121 gtgtgggata tcctttctct gacagcagcg tgctttatcg gtattatagg acgtgcatac
     3181 ctgcttccga ctgtacttgg tgaaaatggt gcatcttcct cagagagtgt atttatcgag
     3241 atgatcaata aactgttttc cagtcatctg ggacttccgt ttatcggagg aatcttcctt
     3301 tgcggtattc ttgcggcaat tatgtccaca gctgattcac agcttctggt aacagcgtcc
     3361 gcagcatctg aggatttata tcatcagttc attaagaagg atgcagactc aaaggaaatc
     3421 cttgcagttg caagacttac ggttatcgtg gtgtctgttc tggcatttgt gatcgcgtgg
     3481 aatccgaaca gcagtatcat gggactggta tccaatgcat gggccggtct tggtgcagcg
     3541 tttggtccga ctgtggtaat gtcactgttc tggagaagaa caaaccttgc aggtgcagtt
     3601 gcaggtattg tttccggtgg tctgacagtt atcgtctggg attatatacc gtttgtcgca
     3661 ggacagactc ttggttctta tacaggactt tattctctgg ctgtaggttt tgctgtcagc
     3721 cttgcgatga ttattatctt cagtctggct acaaaagcac catccaaaga aatcacagat
     3781 gtgtttgata aagtgacagg aaaataatag aatagaaaga ctatccattt tcggatgaat
     3841 ttgttataat ggcagtagcg caaaaaaagc gttactgctt ttttaacctg ataaactgcg
     3901 gagcagttat tcggtgtaga gctgcgtagc gaagcggatg attttatccg cttgattatt
     3961 acaagaagaa ggtgtcgcac atgaagaaat atgattatct gatagtagga gccggccttt
     4021 ttggtgcagt atttgcacat gaggccacaa gacgggggaa aacatgcctt gtgatcgata
     4081 agagaggtca tatcggcggt aatatttaca cagaagaagt agaggggatc caggtacaca
     4141 gatacgctgc acatattttc catacttcta gaaaacaggt gtgggattat atcaatcagt
     4201 ttgcagaatt taatcatttt gtaaattcac cgatcgcagt ctataaggat gagttatata
     4261 atctgccgtt taatatgaat accttccatc agctctgggg agtacgtact cctgcagagg
     4321 caaaagcgaa gatacaggaa cagatcgcaa gaatgcacat tacccatccg aagaacctgg
     4381 aggaacaggc gcttgcactg gttggacagg acgtatatga gaaactggta gagggatata
     4441 caaggaagca gtggggcagg gagtgtaaag atctgccggc atttatcata aaaagactgc
     4501 ctttgcgtta tacttatgat aataactatt tcaaagatcc ttatcaggga atccccaaag
     4561 gtggatatac acgtattgtg aagaaacttc tggagggtgt acaggtatgt ctgaatacag
     4621 atttctttgc aaacagagaa gaactgactg cacaggcaga taaagtactc tttacaggta
     4681 tgatcgatga attttatgac tattgttacg gagaactgga atacagatca ctgagatttg
     4741 agacagaagt gctggatatg ggaaattatc agggcaatgc ggttgtcaat tatacagatt
     4801 atgaagttcc gtatacaagg atcatcgagc ataaacattt tgaatttggt acacagccaa
     4861 agactgtgat caccagagaa tatccggcag catgggagaa aggcaaagag ccgtactatc
     4921 cggtcaatga cccgaagaac agtgaactgt ttgacaaata tgaacgtcgc gcactggaag
     4981 agaagaatgt gatctttggc ggaagacttg gcatgtatcg ctatatggat atggatcagg
     5041 ttattgaaga ggcactgtct ctggcagaaa cagagcttac ctatgaagag ccatgaaagt
     5101 agagttttgg atcgtaagag aaaggtaact gtaaaggcag cagacagttg cagataaagt
     5161 aaccggaaag gacaaatata tgacagacac accaacaatt gctctttatt acagagagca
     5221 tgatccgatg aaaagaaaga tgtttcttga ccagtctatt gcggcaggtg aagatgagga
     5281 ggcaaatgct gtccgtaagg aactgtggga actgcgttac ggagaacctt cagaggcagg
     5341 ctccggtaca cgtgctgatg gatatctggc actttggatg gcaatggaat acagcaagga
     5401 tacagcggga aagctgtttg gtttaaagag ggccagaaaa gaaatcgaga aaaaccttgc
     5461 caggctgaaa ttcagagaaa tgcaggagaa aagtgagctt cacagagaac tgctttacag
     5521 agaatgctgc catatggtca aaacctatat ggaactctgc gagaaggata aggcctataa
     5581 cacgatcttg tgcgggattg tccatatcag tgagaaaagt gcaaagagta agattcagaa
     5641 cgatatttat accactgcca ttgcatttcc ggaggaaatc aacatgcagg aagaactgga
     5701 aatgataaca agggcagcaa gagaagttta tgaggcacat ttcccgggag aaggcggatt
     5761 ataaagaaaa gccaataaaa aattactgga ggcatacatg aacaaagaaa atttgacggc
     5821 ttatgaagtt gtgacagaag aaaatctgac agatatccat tctacaggct ggcttctgcg
     5881 ccataagaaa acaggtgcaa gagttatgct gatcgagaat gatgatgaga ataaagtatt
     5941 taatatcgca ttccgcacac cgcctaagga cagcacaggt gtggcacata tcctggagca
     6001 cagtgtactc tgcggttcaa gggaatttcc attaaaggat cctttcgtag aactggtaaa
     6061 gggttctctg aatacgttcc tgaatgctat gacttatccg gataaaacct gctatcctgt
     6121 agccagctgc aatgataagg attttcagaa tctgatgcat gtttatctgg atgcggtatt
     6181 ttatccgaat atctataaaa gagaagagat tttccgtcag gaaggctgga actatcatct
     6241 ggaacagaag gagggaccgc tgaaatataa cggtgttgta tataatgaga tgaaaggtgc
     6301 tttctcctcg ccggatgaag ttctggaacg ggagattatg aatcaccttt tccctgatac
     6361 cacttatgga tgtgaatcag gcggtgatcc gaaaaatatt ccggatctta cctatgagaa
     6421 tttcctgaat ttccacagaa cttattacca tccatccaac agctatattt atctgtatgg
     6481 aaatatggat atggaagaga aactggcatt cctggatgag cattatctgt cgcattttga
     6541 ttatctggat gtggattctg ttattcagga acagaaagca ttcggagcat gccaggatgt
     6601 gactcttgag tatccggttg ctgagaatga gggagaagag gataatacct atctttctta
     6661 taacatggtt gttggaaatg ctgcggacag tcagatggcg atggcatttg aggtattgga
     6721 ttacgcactg ttaagcgcac cgggagcacc gttaaagcag gcacttctgg atgtaaaagc
     6781 aggcaaggat gtctatggat cttatgatga tggtattctc cagccgtatt ttacagtaat
     6841 tgcaaaaggc tccaatccgg acagaaaaga agaattcgta tctgtgatca gacaggtact
     6901 tggagatatt gttaagaatg gaattgacag aaaagctgta gaggcaggaa tcaattactt
     6961 tgaattccgc tatcgtgaag ctgatttttc ctcttatccg aaaggactga tgtacagtct
     7021 ggatattctg ggagactggc tgtatgaaaa aggaaatccc tttgcacagg tacagcagct
     7081 tacagttttt gaaaaactga aaaaggcagt aaacgaagga tattttgaag aactgatccg
     7141 taaatatctt cttgagaatc cacatggatg catcatgact cttgtgccga agaaaggtct
     7201 ggcagcccag cgtgagaaag agctggagga gaagctggaa gcatacagaa gcagtctttc
     7261 tgaagaacag ctggatgcaa tggttgagaa gacgaaagca ctggaagcat atcaggaagc
     7321 cggcgaagat ccaaaagcac tggagtgcat tccgatgtta aagagaagtg atattaaaag
     7381 agaagctgca aagatcataa atgaagaatt aacggtagat gacagtctgt tcctttacca
     7441 tgacgtatgc acaaatggca tcggttacgt agacctgatg tttaagactg acagtattgc
     7501 gcctgagcag attccttatc tgggactttt gaaatccgta ctgggttatg tggatacaga
     7561 gaattacaca tacggagaac tgttcaatga aatcaatgcc aatacaggcg gtatcaactg
     7621 tggggtagag gtttttgaca gagctgattc tacagaagag tttcaggcta tgttctctgt
     7681 aagaggtaag gcactttata cgaaaatgga tttccttttc aaaatgatcg gggagatcct
     7741 gaacagctcg aaactggagg ataccaagcg tctctatgag attgttgcat ctgtgaagtc
     7801 cagggcacag gtaaacctga caggtgccgg acattccact gctgtactca gagcagctgc
     7861 atattcttct ccgatggctg catttcagga tgagatggcg ggaatcggat attatcagtt
     7921 tatcgagaaa ctggaaaaag attttgaaca gcgaaaagag gaaactgtag aagagctttg
     7981 caaactgatg aaaaaaattc tccgtccgga gaactttatg atcagttata caggtgagag
     8041 agagtccctg gagacagttc agaaactggc gggagctgta aaggcaggac ttggcacaga
     8101 gccggtggag aaatcggaag agaagctgac ctgcacaaag aagaatgagg gatttaagac
     8161 ttccggacag gtccagtatg tggctcagac cggtaacttt aagaaaaaag gcctggaata
     8221 tacaggtgca ctggaaattc tgaaggttat cttaagttat gattatctgt ggatgaatct
     8281 gcgcgtaaag ggcggtgcat atggatgtat gagcggattt aagaggaatg gtgagagcta
     8341 tctggtatct tatcgcgatc ctcatctgaa acgtacactg gatgtatata aggggattcc
     8401 ggattatatc cgcaattttc aggcagatga gcgcgagatg acaaaatata tcattggtac
     8461 catcagcgga aaggatgttc ccaggacacc tcagatgaaa ggttctgttt ccaagacagc
     8521 atatttctgc ggtgtgacag aggagatgct ccagaaagaa cgtgatcaga ttctgaatgc
     8581 gtctgcagaa gatatccatg cactggccga gatcatagag gcagttctgg cagcagatca
     8641 gatatgtgtg gtgggaagcg agagcaaggt ggctgaggcc tctgatgtac tgatggaagt
     8701 gaaaccactg atcaattgtt aaaaaaggaa agaacagaat gaacgaaaat tttaaatccg
     8761 gatttgtagc gattattgga cgtccaaacg taggtaaatc cactctgatg aatcatctca
     8821 tcggacagaa gatcgctatc acatccaaga aaccacagac aaccagaaac cgtatccaga
     8881 ccgtttatac ctgtgacgaa ggtcagatcg tatttctcga tactcccggt atccacaagg
     8941 caaagaataa gctgggagaa tatatggtcc aggtggcaga gcgaacttta aaagaggtag
     9001 atgccattat gtggctggta gagccaagta cctttatcgg ggcaggagag cgtcatattg
     9061 cagaacagct tcagggaatc ggagtgccgg taatcctgat catcaacaaa attgatactg
     9121 tatcaaagga agaaatcctt cctgccattg acacctaccg aaaagtatgc gatttcgcag
     9181 agattatccc atgctccgca ctgcgtggac agaatacaca ggatattatc ggttgtatcc
     9241 tgaaatatct gccttacggt cctatgtttt atgacgaaga tacagttact gatcagcctc
     9301 agcgtcagat cgtggctgag atcatccgcg agaaagcact tcatgcactg gatgcggaaa
     9361 ttccgcatgg aatcgcagtt gccattgacc ggatgaaaac acgtccggga aaaaatccaa
     9421 tcgtggatat tgatgcgaca attatctgtg aaagagagtc tcacaaagga attatcatcg
     9481 gcaaacaggg tgcgatgtta aagaaaatcg gaagtaatgc aagatatgag attgagcgta
     9541 tgctggaatc aaaagtgaat ctgaagcttt gggtaaaagt aaagaaagac tggagagaca
     9601 gtgatttcct tatcaagaac tttggttacg ataagaagga aatctaaata aaaagacagt
     9661 caggaaacag agagaatgag tgatttgata actgtgcagg gagtagtgct ctctgccatg
     9721 cctgtgggag aatatgacaa gcggatcgtc cttctcacca gagaaagagg caagatttcc
     9781 gcgtttgcaa aaggagcccg ccgcccaaac agccagtttc tggcggctgc caatcctttt
     9841 gtgtttggaa cctttgccct ttatgagggg cgttccagtt ataatctgaa tcaggtatcc
     9901 atcagtcatc attttgtgga actggcaggc gaacagcctg gaatctacta tggatattat
     9961 tttctggaac tggcagatta tttcggacag gaggggattg acgagaaaga atctatgaac
    10021 cttctgtatg tgacagtgaa ggcacttctg aacccgaata ttgataacag gctggtgagg
    10081 tgtatttttg aacttcgtat gatggctgca cagggattgt gtccttcact gtttcattgt
    10141 gtgtgctgcg aaagacagcc tgtggaggga gaagagcttt tcttttctca gcagaatcat
    10201 ggtattctgg ataaagcctg tctgggacat gtaaatgatg caaagagaat ctcagcacct
    10261 gcattatatg ccatgcagta catagtaact gcttctcttg ggaagcttta tacatttact
    10321 gttacagaag aagtactcca tgaactggaa cgtcatatcc atacctatat tgctgcgaac
    10381 actgaaaaac ggttcaaaag tctggaaatt ctggaaataa tgagctgaga taccttgaaa
    10441 aagaaatcag atacagttat aataacgtta attgtagctg tttattacgt taatcaaata
    10501 gcggaatgga ttgtgaacca aattgcctga cggatatgaa cagttacacg cattttataa
    10561 aaagaacgag gagaatagtc atggaaaaaa caatggacaa aatcattgcg ctggcaaagg
    10621 caagaggatt tgtttacccg ggttccgaaa tctatggtgg tcttgcaaac acatgggatt
    10681 acggtaatct tggtgtagaa ttaaagaata acgtaaaacg tgcatggtgg cagaaattta
    10741 ttcaggaatc cccatacaat gtaggtgttg actgtgcaat tctgatgaac ccgcagacat
    10801 gggttgcatc cggacatctg ggaagcttct ctgacccgct gatggactgt aaggaatgtc
    10861 atgagcgttt ccgcgcagac aagatcattg aggatttcag ccaggagaac ggaattgaac
    10921 tggagagctc tgtagatgga tggtctcagg aagaaatgat gaacttcatt aaagatcaca
    10981 atgtaccatg ccctacatgc ggcaaacata actttacaga tatccgtcag ttcaacctga
    11041 tgttcaaaac attccagggt gttacagagg atgccaaga
//
LOCUS       DBMDXG010000019        10810 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1705_length_10810_cov_1.912690, whole genome shotgun sequence.
ACCESSION   DBMDXG010000019 DBMDXG010000000
VERSION     DBMDXG010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10810)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10810)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10810
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1705_length_10810_cov_1.912690"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(16..348)
                     /locus_tag="ACZPD3_01305"
     CDS             complement(16..348)
                     /locus_tag="ACZPD3_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018877.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR1414682.1"
                     /translation="MKGKIKMENSGKKYQIDTEENKMFLGELQKNLLNFGKGFLSPGG
                     SAYFLGDDGTPWKDRNRETWITCRMVHVYSMGIMLGDKESPALVHGAVHGLLEELKDR
                     ENGGWYLV"
     gene            complement(414..485)
                     /locus_tag="ACZPD3_01310"
     tRNA            complement(414..485)
                     /locus_tag="ACZPD3_01310"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gln,seq:ctg)
     gene            948..2411
                     /locus_tag="ACZPD3_01315"
     CDS             948..2411
                     /locus_tag="ACZPD3_01315"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR1414683.1"
                     /translation="MRANNLTLLTDLYELTMMQGYFKNPTNQTVIFDMFYRTNPCGGA
                     FAITAGLEQMIEYIENLKFTDEDIEYLRSLNTFEEDFLEYLSNFRFTGDIYAIPEGTV
                     VFPREPLVKVVAPIMEAQLVETAILNIINHQSLIATKAARVCYAAKGDAIMEFGLRRA
                     QGPDAGIFGARAAIIGGCAGTSCVLTGKMFDVPVLGTHAHSWIMSFPDEYTAFKTYAK
                     LYPNACTLLVDTYDVLKSGVPNAIRVFEEMREEGIPLTKYGIRIDSGDLAYLSKEAYK
                     MLAAAGFDDAVISASSDLDEYLIESLKAQDAKINSWGVGTRLITANDNPAFGGVYKLA
                     AVKDADSTEFTPKIKLSENTEKVTNPGNKTVYRLYNKKTGKIRADLICLADEKLDADQ
                     NMVLFDPIDTWKKTKVLGGTYEVRELLVPVIKEGKRVYESPSVMELRDYCQKEQNTLW
                     DESRRFVNPQKVYVDLSQKLWDLKKDLLEEISEKALD"
     gene            complement(2507..3709)
                     /locus_tag="ACZPD3_01320"
     CDS             complement(2507..3709)
                     /locus_tag="ACZPD3_01320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1414684.1"
                     /translation="MAAIYLAPFYLLVCVYILLRSVHWFQVLHTVFQNVWVCRGIGLV
                     YLFVVFSILIAFMAPASGFRRFMKLLSNYWLGVLMYTLMTLGIADGLRLLLKYPLRNF
                     AFPGRELLFSNMGTAVVGAVCAVIISTVSIYGVLSVGNIHTTKYNISVDKKAGNMKEL
                     NVVLVADLHLGYNIGCKQMEQMTEKINEQKPDLVVVAGDIFDNEYEALDDPEKLAEIL
                     RGIRSKYGVYACYGNHDIQEKILAGFTFGSKEKKESTPEMDEFLEKAGITLLRDEYVL
                     IDDSFYLYGRPDYERPGRGIDKRKTPQEITEGMDVSLPVLVIDHEPRELQELADTGVD
                     VDLCGHTHDGQVFPGNIVIRFFWENPCGYLKKGNMHNIVTSGVGLFGPNMRVGTKSEI
                     CDVSICFN"
     gene            complement(3900..4448)
                     /gene="spoVT"
                     /locus_tag="ACZPD3_01325"
     CDS             complement(3900..4448)
                     /gene="spoVT"
                     /locus_tag="ACZPD3_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HIR1414685.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIKEGTPLEIFTDREGEI
                     ILKKYSPIGELNVFAKEYAEALAQSSGMVACITDHDQVVAAAGQGSREYMGKPISKAL
                     EDAITERSSVFANGNDRSRIPVTQEQREPLYSQIMQPIISAGDTVGSVLLLGKNEKDV
                     MGESEKMLIRTASGFLGRQMEQ"
     gene            complement(4605..>7138)
                     /locus_tag="ACZPD3_01330"
     CDS             complement(4605..>7138)
                     /locus_tag="ACZPD3_01330"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR1414686.1"
                     /translation="TGAKQGIELKVSKFTCAANTEQYKICFYDKKIYIENVEELLPVK
                     DFLDSLSLIPGEKAFIENKDVPAFCQELLPVIQKFFKCRMVEFHPENYGMVKPEFRFY
                     LDAPQENMITCKATVKYGDREFSLYTTDDIAARDMNRETVVRNVIHKYSNAFNPFEQC
                     AVIADDEEMEYEFLTEGIQALQAVGEVFISDALRRIEVRNSPKVTVGVSLSGNLLELS
                     MTAGDISKEELIDILSRYNKKKKFYRLKNGAFVNAADSGLDTVEELRAGLQLTDKQMK
                     QDKIEVQKYRALYLDAQLKENPVVSAVKDKSFKSLVRNMKTIEDNDFEVPESLDKVLR
                     EYQKRGFLWIKTLNYNGFGGILADDMGLGKTLQVIAFLLSEFLERRNTVVENIAVKET
                     AMLNMQSEIETVEQACKAAEAQKADGKKADGQTGKLQKNTLIIAPASLVYNWSSEIQR
                     FAPELTAKMVTGTAAERRQILAEADSEDILLTSYDLLKRDISEYEGYKFRCEIIDEAQ
                     YIKNANTQAAKAVKEVQADFRLALTGTPVENRLSELWSIFDYLMPGFLYSYKKFREEV
                     EIPAVQNSDEDAMKRLQKMIRPFVLRRLKKEVLTDLPDKLEENMFVQLTGEQQKLYDA
                     HVKRMMLMLDKQSEEEFKTSKITILAELTKLRQICCDPSLIFADYKADSAKVDMCLNM
                     ISNAVESGHKILLFSQFTTMLDHLAKRLEEEKISYYMLTGSTSKEKRAQMVENFNTDD
                     TQVFCISLKAGGTGLNLTAADIVIHFDPWWNLAVQNQATDRAHRIGQKNVVNVYKLIV
                     KDTIEENILKLQEKKRELADQILEGEGLNGGSFTKEELMELLSGK"
     assembly_gap    7139..7148
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7149..8043)
                     /locus_tag="ACZPD3_01335"
     CDS             complement(<7149..8043)
                     /locus_tag="ACZPD3_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016330.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR1414687.1"
                     /translation="MISKTQIRYMANSSSYSKGAELYATGKVLDMDVKNMGASDEIVA
                     SVKGSGRNIYEVDVSIDTENDEVDTCYCECRAYAEYGGLCKHCVAVLLQYNDYENDMD
                     SYDYGQSVEKIVKGGLTHTIRKGVQMHTTPELASLLQKQAVAKSLPLIQGSTYGKVRL
                     EPYFNFDGRTFTVEFKIGINKMYVLKDAFSFDVHIANQDDYKYGKNLQFVHTIESFAE
                     ESRPLAKFICKWADNNRQFHRSSSYYGYYMGTLEKVRHLELSGNELAEFLLLMEGKTL
                     QGESIGTRNTTWEITREHLPRK"
     gene            complement(8185..10209)
                     /locus_tag="ACZPD3_01340"
     CDS             complement(8185..10209)
                     /locus_tag="ACZPD3_01340"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR1414688.1"
                     /translation="MDFRTDKLLHGGDYNPEQWLKRPDILEKDIDMLEESGCNVVSLG
                     IFSWSTLEPEEGVFHFGWLQEIIDKLYKRGISTILATPSGARPKWMADKYPEVLRVDE
                     TRHRALFGFRHNHCYTSPVYREKVHIINKKLAQEVATHPGVILWHISNEYGGECHCPL
                     CQEAFRNWLKEKYQTIENLNDQWCTTFWSHTYNSFDQIESPSKIGETQLHALNLDWKR
                     FVTHQTADFIHHEIAALREGGSTLPTTANLMHYFGGLDYFKIAKEIDVVSWDTYPTWH
                     KEAVIDTAYDNGMCHDLMRSLKGKPFFQMESCPTSTNWQSVSKLKKPGMLFAQSMQAI
                     AHGGEGALYFQIRQSRGASEKFHGAVIDHYGGNDTRVFKEVSRVGEVLKELKELAGTT
                     VNSSVAMLYDWDSQWAMEDSQGPRNKGLHYLEAMLKFYRGFRKQGVNVDVIDMTCELD
                     KYKVLALPMVYMFKEGFAKKIRAFVENGGTLITSYWSGIADDTDRCYLEGTPHGLMDV
                     LGIRSTEIDGLYDWEENSFVPVAGNELGLDKTYTCKYLCDLVELRGARTLMTYGSDFY
                     EGYSCLTVNEYGKGKAWYVAADADKEFYGDFLEKVLKDSGVSCGIKEEIPDALEITVR
                     ECENEKYYIYQNFGTEAVSIPVPEGEISWIYGNGSDKLKVYGLAVAKVIV"
BASE COUNT         2940 a         2719 c         1754 g         3387 t
ORIGIN      
        1 gatggtatcc gggaattaca ccagatacca tcctccattt tcacgatctt tcagttcttc
       61 cagtaatcca tgtacagcac catggaccag tgccggactt tctttatccc caagcatgat
      121 tcccatacta tatacatgga ccatacggca tgtgatccat gtttctctgt ttctgtcttt
      181 ccatggtgtt ccatcatccc caaggaaata agcacttcct cccggtgata aaaatccttt
      241 tccaaaattt aaaagatttt tctgcaattc acctaagaac attttatttt cttctgtatc
      301 tatctgatat tttttacccg aattttccat cttaatctta cctttcactt ccaaaaaatt
      361 tcattttctc ttatagcaca aaaaagcacc tcataccgag gtgcctcatg tagcagggct
      421 acaaggattc gaaccttgaa ctgacggagt cagagtccgt tgccttaccg tttggcgata
      481 gccctttatg ttttgttttc gctacttcgt tcgcgacaat tggtattata taatagtctt
      541 tcaaaaaatg caaccccttt tttgaaattt ttttaatttt ttttattttt cttcaaaaac
      601 atgaaaatac cgcctatttc ccggagtttt tttatgtact ctgggaaaaa ttatgctata
      661 atcaataaaa acttattgct gcttctgata tactctttct taaatataac aattctcgta
      721 actggtgtgc cgacaggttg acgcaagatt gtttcaagaa tcaacttgtc ggcacactag
      781 aaacttgcta tagagatcaa agcagcagga aatcttaata ataatttctc cagactatat
      841 attatgatac tgaggtcaaa agctattcag ccctggcctg atatctgtaa attgcgataa
      901 acagttacac ccgagatatt attatgaacc caaaaggaga gctatatatg agagctaata
      961 atcttactct gctcacagac ctttatgaac ttactatgat gcagggctat tttaagaacc
     1021 caacaaacca gactgttatc ttcgatatgt tttaccgtac caatccatgc ggaggtgcat
     1081 ttgccatcac tgccggactg gaacagatga ttgaatatat tgagaatctt aaattcacag
     1141 atgaagatat cgaatatctc agaagtctga atacttttga agaggacttc cttgaatatt
     1201 taagcaactt ccgtttcaca ggtgatatct atgcaattcc tgagggaaca gttgtcttcc
     1261 caagagaacc gcttgtcaaa gttgtagcac ctatcatgga agctcagctt gtagagacag
     1321 caatcctgaa tatcatcaat caccagagcc tcattgctac caaagcagcc cgcgtttgct
     1381 atgctgcaaa gggtgatgcg atcatggaat ttggtctgcg tcgcgcccag ggaccggatg
     1441 caggtatctt tggcgcacgt gccgcaatca tcggtggatg tgccggtact tcctgtgttc
     1501 tcacaggcaa aatgttcgac gtaccggtcc ttggaactca cgcacacagc tggatcatga
     1561 gcttcccgga tgaatatacc gcattcaaaa cctatgccaa actttatccg aatgcctgca
     1621 ccttactggt agatacctat gatgtcctga aatccggtgt tccaaatgca attcgtgtat
     1681 ttgaggaaat gcgcgaagaa ggtattccgc ttaccaaata cggtatccgt atcgacagtg
     1741 gtgatcttgc ctacctttcc aaagaagcat ataagatgct tgctgctgcc ggatttgacg
     1801 atgcagttat ttccgcttcc agcgatctgg acgaatatct cattgagagc ctgaaggctc
     1861 aggatgccaa gattaattcc tggggtgtcg gaacacgact gatcaccgct aatgataatc
     1921 ctgcattcgg cggtgtatac aaacttgctg ctgtcaaaga tgcagacagt accgaattca
     1981 cacctaagat caagctttcc gaaaacacag agaaagtaac caaccctgga aataaaaccg
     2041 tatatcgtct ctataataag aaaaccggaa aaatcagagc agatcttatc tgccttgctg
     2101 atgaaaagct tgatgcagat cagaacatgg ttctctttga tccaatcgat acatggaaaa
     2161 aaacaaaagt tctcggcgga acctatgaag tacgcgaact tctcgttcct gttatcaaag
     2221 aaggtaaacg agtctatgaa tctccatctg ttatggagct tcgcgattac tgccagaaag
     2281 agcagaatac tctctgggat gaatccagac gtttcgtaaa tcctcagaaa gtatatgtag
     2341 acctttctca gaaactctgg gatctgaaaa aagaccttct ggaagaaatt agtgagaaag
     2401 ctctcgactg agcataaact cagattaaga gtaacaaaac agacaagtcc ccggctccaa
     2461 ccgcaaaaat caacaggcag ttgaagccgg ggacttactc tttgattcag ttaaaacata
     2521 tgctgacatc acagatttcg cttttagtcc caactctcat atttggtcca aaaagcccta
     2581 caccggatgt aacgatatta tgcatatttc ctttcttcag ataaccacag ggattttccc
     2641 agaagaatct gatcacaata tttcccggaa atacctgtcc gtcatgggta tgtccgcaga
     2701 gatctacgtc cacacctgta tccgccagtt cctgaagttc tctcggctca tgatcgataa
     2761 ccaacactgg cagtgacaca tccataccct ctgtgatttc ctgcggagtc tttcgtttat
     2821 caatcccccg tccgggacgc tcataatccg gtcttccata cagataaaag gaatcatcaa
     2881 tgagcacata ctcatctctc agaagcgtaa tccccgcttt ctccagaaac tcatccatct
     2941 ccggggtact ctctttcttt tccttactgc caaaggtaaa tcccgccaga atcttttcct
     3001 ggatatcgtg gtttccgtaa catgcataca cgccatactt cgaccgaatc cctctcaata
     3061 tctccgccag tttttccgga tcatccagtg cctcatattc attgtcaaaa atatctcctg
     3121 ccactaccac cagatccggt ttctgctcat tgatcttctc tgtcatctgc tccatctgct
     3181 tgcatccaat attatatccc agatgaagat ctgcgaccag aaccacattt aactccttca
     3241 tatttcctgc ctttttatca acagaaatat tgtacttcgt tgtatgtata tttccaacac
     3301 tgagcactcc gtaaatactg actgttgaaa tgatcaccgc acagacagct cccacaacag
     3361 cagttcccat attactgaaa agcaattctc tccccggaaa ggcaaaattt ctaagaggat
     3421 atttcagtaa cagtctcaat ccatccgcga ttcccagagt cataagtgta tacatcaaaa
     3481 caccgagcca gtaattactc agaagcttca taaatctcct gaatcctgat gcaggtgcca
     3541 taaatgcgat cagtatacta aacactacaa ataaatatac aagtcctatc cccctgcaca
     3601 cccacacatt ctgaaaaacc gtatgcagta cctgaaacca gtgcacgctg cgaagcagga
     3661 tatatacaca caccaacaga taaaacggtg ccagatatat tgcagccata taatatatct
     3721 cctttccaac tctcattacc atgacaattt cattactata aacaactcca gaaaatcata
     3781 actgcttctt attatacaaa aaagaatcct cagccgccac cataagctgc tgaagattct
     3841 atgaattatt atttttttat tatgattgcg cactacatcc caaatatatg ctacagattt
     3901 tactgttcca tctgtcttcc cagaaatccg cttgctgttc tgatgagcat cttctctgat
     3961 tcacccatca catccttttc attctttccc agtaaaagca cagatcctac tgtatctcct
     4021 gcactgataa taggctgcat gatctgggaa tacaaaggtt ctctctgttc ctgagtcaca
     4081 ggtatcctgc ttctgtcatt tccgttagca aacacagagc ttctttccgt gattgcatct
     4141 tccagagctt tgctgatagg ttttcccata tattccctgc ttccctgtcc cgcagcagcc
     4201 actacctgat cgtgatctgt gatacacgct accatcccgc ttgactgcgc cagcgcctct
     4261 gcatattcct ttgcaaagac attcagttct ccgatagggg aatacttttt cagaataatc
     4321 tccccctccc ggtcagtaaa aatctccagt ggtgtcccct cttttatccg cagcgttttt
     4381 ctgatctcct taggaatgac cacacgcccc agatcatcta tgcgccgaac aattcctgtc
     4441 gctttcatgc acttttcttc ctcctgtttt tccttttatc gaagtttact gtctttgtac
     4501 ccttcagttt ccatatcatg tgttgtatgg ttattatgtg gaaaaacagg cagaatatac
     4561 atttctgtat ttacaaaaaa tgccatttaa ctgcaatcaa gcggttattt cccggaaagt
     4621 aattccatca gttcctcttt tgtgaagctg cctccgttaa gcccttcgcc ctccaggatc
     4681 tgatctgcca gttcacgttt cttttcctgc agtttcaaaa tattttcttc aatcgtatcc
     4741 ttcacaatca gcttgtatac attcactaca ttcttctgac caattctgtg tgctctgtcc
     4801 gttgcctgat tctgcactgc cagattccac catgggtcaa aatggattac aatatctgct
     4861 gccgtcagat tcagaccggt tcctcctgcc ttcagagaaa tacagaatac ctgtgtgtca
     4921 tctgtgttaa agttctctac catctgtgca cgcttctcct tacttgtaga accagtcagc
     4981 atatagtaac tgatcttctc ctcttcaagg cgcttcgcaa gatggtcaag cattgtcgta
     5041 aactgcgaaa acaacagaat cttatgtccg ctctccactg cattactgat catattcagg
     5101 cacatatcca cttttgcaga atctgcttta taatctgcaa aaataagaga cggatcacaa
     5161 cagatctggc gcaattttgt cagctctgcc agaattgtaa tctttgaggt cttaaattct
     5221 tcttcggact gcttatccag catcagcatc atccgtttca catgtgcatc ataaagcttc
     5281 tgctgctctc ctgttagctg tacaaacata ttctcttcca gcttatccgg cagatctgta
     5341 agcacttcct tcttcaatct tcggagtaca aacggtcgga tcattttctg cagacgtttc
     5401 attgcatctt catctgaatt ctgaactgcc ggaatttcaa cttcttccct gaacttctta
     5461 taactgtaaa ggaaaccggg catcagataa tcaaatatac tccacaattc actcaggcgg
     5521 ttttccaccg gagttcctgt aagagccagc ctgaagtctg cctgaacctc ttttactgct
     5581 ttcgcagcct gagtgtttgc attcttgata tactgagctt catcaataat ctcacatctg
     5641 aacttatatc cttcatattc gctgatatcc cttttcagca aatcatagga agtcagaaga
     5701 atatcctcag aatctgcttc tgccaggatc tgtcttctct ctgctgcagt cccggtcacc
     5761 atctttgctg taagctccgg tgcaaaacgt tgaatttcac tgctccagtt atacaccaat
     5821 gatgcaggag ctataattaa tgtatttttt tgcagctttc cagtttgtcc atctgccttc
     5881 tttccatcag ctttctgcgc ctctgctgcc ttgcacgcct gttccactgt ctcaatttca
     5941 ctctgcatat ttaacattgc agtttctttt actgctatat tttcaacaac agtattcctt
     6001 ctttccagaa attctgacag tagaaatgca atcacctgca acgtttttcc aagtcccata
     6061 tcatctgcaa gtattccgcc gaatccatta taattcaatg tcttaatcca cagaaatccc
     6121 cgtttctgat actcccgcag aactttatcc aggctctccg gcacctcaaa atcattatcc
     6181 tcaatggtct tcatatttct taccagcgac ttaaaagatt tatcctttac tgccgaaaca
     6241 accggattct ctttcaactg tgcatccaaa tacaacgcac ggtacttctg tacttcaatc
     6301 ttatcctgct tcatctgctt atccgtcagc tgaagccctg ccctgagttc ttccactgta
     6361 tccagacccg agtctgctgc atttacaaaa gctccgtttt tcagacgata gaactttttc
     6421 ttcttattat atctggaaag aatgtcaatc agttcttcct ttgaaatatc tcctgctgtc
     6481 atactcagct ccagcagatt tccactcagg gaaacaccta ctgtaacctt tggcgaattc
     6541 cggacttcaa tccgtctcag cgcatcagaa ataaatactt ctcccactgc ctgcagtgcc
     6601 tgaattcctt ctgtcagaaa ttcatattcc atctcttcat catccgcaat taccgcacac
     6661 tgttcaaatg gattaaaagc attcgaatat ttatggatca catttctgac aactgtttct
     6721 ctattcatat cccttgctgc aatatcgtcc gttgtatata aagaaaattc cctgtctcca
     6781 tacttaacag tcgccttaca tgtaatcata ttttcctgcg gtgcatccag ataaaatcga
     6841 aactccggtt taaccattcc ataattttcc ggatgaaatt caaccattct gcatttaaaa
     6901 aacttctgga tcaccggtaa aagttcctga cagaatgccg gtacatcttt attttctata
     6961 aacgctttct caccgggaat caaagacaaa ctgtccagaa aatccttaac aggtaaaagc
     7021 tcttctacat tctcaatata aatcttttta tcataaaagc aaattttata ctgttccgta
     7081 tttgctgcac acgtaaattt gcttaccttc agttcaattc cctgctttgc acctgttann
     7141 nnnnnnnntt tttctcggaa gatgttctct ggttatttcc caggttgtgt ttctggttcc
     7201 tatagattct ccctgcaatg tctttccttc catcaaaagc aaaaattctg ccagttcatt
     7261 tcctgacagc tccagatgcc ttaccttttc cagagttccc atatagtatc cataatagga
     7321 agaggatctg tgaaattgtc tgttgttgtc tgcccattta cagataaatt tcgcaagcgg
     7381 tctggattcc tcggcaaagg attctattgt atggacaaac tgaagatttt tcccatattt
     7441 ataatcatcc tgatttgcta tatgaacatc aaaggaaaat gcatccttca gtacgtacat
     7501 tttattaatt cctattttaa attctactgt aaatgttctt ccgtcaaaat taaaatatgg
     7561 ttccagcctt acttttccat aagtgcttcc ctgaatcagt ggaagagact tcgcaactgc
     7621 ctgcttctgc aataatgaag ccaattccgg cgtggtatgc atctgcactc cttttctgat
     7681 cgtatgggtc aggccacctt ttacaatttt ttctacagat tgtccgtaat cataactatc
     7741 catatcattt tcataatcat tatattgcag aagaaccgct acacaatgtt tacaaagtcc
     7801 accatattct gcatatgccc gacattcaca atagcaggta tcaacttcat cattctctgt
     7861 atcgattgat acatctactt catagatatt tcttccactg ccttttacag atgcaacaat
     7921 ttcatcagac gcacccatat tttttacatc catatccaaa acttttcctg ttgcataaag
     7981 ttctgcacct ttactgtatg atgaagaatt tgccatatat ctgatctgag tttttgaaat
     8041 catatttcta tacctttcct gatacaatta aaaacttttt gtatcatttt cccggttaaa
     8101 cctgagtaaa aaaagaatcc cttcaccagg cagtttcaat ctctgcttaa agtgaaggga
     8161 tttcctcatc catcgttatt tattttacac gataaccttc gcaactgcaa gaccataaac
     8221 tttaagttta tcgctgccat tgccataaat ccaactgatc tcgccttccg gtacgggaat
     8281 gcttacagct tctgttccga aattctgata aatataatat ttctcatttt cacactctct
     8341 cactgtaatc tccagtgcat ccgggatttc ttctttgatt ccgcaggaaa cgccgctgtc
     8401 tttgagcact ttttcaagga agtctccata gaattcttta tcagcatctg ctgccacata
     8461 ccatgcctta cctttgccat attcattcac tgtcaggcag gaatagcctt catagaaatc
     8521 acttccgtag gtcattaaag tacgtgctcc gcgaagctcc acaaggtcgc acagatattt
     8581 acatgtataa gtcttatcca gtccaagttc attgccagcc acaggaacaa aagaattttc
     8641 ttcccagtca taaaggccgt caatttctgt gcttctgatt cccagcacat ccatcagacc
     8701 atgaggagtt ccttccagat aacacctgtc tgtgtcatct gcaattccgg accagtaact
     8761 tgtgatcagt gtaccgccgt tttccacgaa cgcacgaatc tttttggcaa aaccttcctt
     8821 gaacatataa accattggaa gagccagtac tttatattta tccagttcac aggtcatatc
     8881 gatcacatcc acattgacgc cctgcttacg gaaacctcta tagaatttca gcattgcttc
     8941 cagataatga agccctttat ttctgggtcc ctggctgtcc tccattgccc actggctgtc
     9001 ccagtcataa agcatggcaa ccgagctgtt cacagttgtt cctgccagtt cttttaactc
     9061 tttcagaact tctccaaccc tggaaacttc tttaaacact cttgtatcat ttccaccgta
     9121 atgatcgatc actgctccgt ggaacttctc agaagcacct ctgctctggc ggatctggaa
     9181 ataaagagca ccctctccgc catgtgcaat tgcctgcatg gactgtgcaa aaagcattcc
     9241 cggtttcttt aatttgctca cactctgcca gtttgtagaa gtcgggcagg attccatctg
     9301 gaaaaacggc tttcctttca gagaacgcat caggtcatgg cacattccat tgtcataagc
     9361 tgtatcgatc acagcctctt tatgccatgt aggataggta tcccaggaaa ccacatctat
     9421 ctctttcgca atcttaaaat aatctaaacc tccaaaataa tgcatcaggt tagcagtagt
     9481 cggaagtgtg cttcctccct ctctcagtgc tgcaatctca tgatgaatga aatcagcagt
     9541 ctgatgagtg acaaagcgtt tccagtccag attcagtgca tgaagctgtg tctcaccaat
     9601 cttagacgga ctctcaatct ggtcaaagct gttataggta tgactccaga aagtggtaca
     9661 ccactgatca ttcaggtttt caatagtctg gtatttctct ttcaaccagt tacgaaatgc
     9721 ttcctgacac aatgggcagt gacattctcc gccatattca ttggaaatat gccacaggat
     9781 cacacccgga tgtgtggcaa cctcctgcgc cagctttttg ttaataatat gtaccttctc
     9841 tctgtaaaca ggagaagtat aacaatgatt atgtctgaat ccaaataacg ctctgtgacg
     9901 cgtttcatct acacgaagta cctccggata cttatctgcc atccattttg gtcttgcacc
     9961 tgacggtgta gccaggattg tagaaattcc tcttttataa agcttatcaa tgatctcctg
    10021 cagccatcca aaatgaaata caccttcttc cggctccagt gtggaccagg aaaaaatccc
    10081 aaggcttact acattacagc cagactcctc cagcatatca atatcttttt ccagaatatc
    10141 cggtctcttt aaccactgtt ccgggttgta atcaccgcca tggagcagct tatccgttct
    10201 aaagtccata cctgttctcc tcttaactct ctattttcaa tcgttttctt tttcattatc
    10261 atatttaaac tctatccaat ttcaaaacac tctcttctcg ctgctcatta cttttctcag
    10321 tcaaagcaga cgggatttga tttcattctt cttacagaat atggtttgca attactccaa
    10381 gtttgtttgc gcaaccgatt gttcatattt ttagtatatt cctttatatt aataaagtca
    10441 attagccatt attatctaat ttcgaatctc ttttttgtca tatcacacaa ttcatgactg
    10501 ctttttccat ttttcaaaca cacacacgaa tcctgcttgc aggattcctc cgcctgtggc
    10561 gagtcgcttc agcgacataa ttcctctctt ccacaatgcg atcctgcccg ggaacttttt
    10621 atgttacaga aacattatga agtaatttcc catgaaaaaa atcctctgat tattgattct
    10681 ccaataatta cagaggattt ttcatttttc tactgtgttg actcacgcag catcacctga
    10741 taattcagtg tcaacgtatc ctccaaaggc ttctccccga ggatgttcag taatttcgca
    10801 catgccatct
//
LOCUS       DBMDXG010000020        10622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1745_length_10622_cov_1.819979, whole genome shotgun sequence.
ACCESSION   DBMDXG010000020 DBMDXG010000000
VERSION     DBMDXG010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_10622_cov_1.819979"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="ACZPD3_01345"
     CDS             <1..341
                     /locus_tag="ACZPD3_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414689.1"
                     /translation="ASIISICYSKIIRILFCLTKDKIKYKYFFVKECDFMTAVKERII
                     GAVSIMSDKDANIFWHIIQKHFKLPDTFADIEKVEPDETDLIMLKEIENNPDCHEFIS
                     QEELMKELNM"
     gene            512..584
                     /locus_tag="ACZPD3_01350"
     tRNA            512..584
                     /locus_tag="ACZPD3_01350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:545..547,aa:Gly,seq:gcc)
     gene            complement(976..2160)
                     /locus_tag="ACZPD3_01355"
     CDS             complement(976..2160)
                     /locus_tag="ACZPD3_01355"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HIR1414690.1"
                     /translation="MKEFELKYGCNPNQKPAKIYMADGSELPVKILSGRPGYINFLDA
                     FNGWQLVSNLKKATGLPAATSFKHVSPAGAAVGLPLTETLAKIYWVNDMDWKNFSPLA
                     CAYARARGADRMSSFGDFISLSDVCDKDTALLIKREVSDGVIAPGYTEEALEILKQKK
                     KGNYNVIQIDENFVPAPLEHKEVFGVTFEQGRQELEIDDAMLSNIVTENKELTEEAKR
                     DMKISLIILKYTQSNSVCFVKDGQAIGVGAGQQSRVHCTRLAGQKADNWFLRQCPKVL
                     NLPFKDTISRAERDNAIDVYIGDEYMDVLADGMWEKTFTEKPEVFTKEEKRAWLDQMT
                     DVTLGSDAFFPFSDNIERAHKSGVKYIAQPGGSVRDDAVIETCNKYGMVMSFTGIRLF
                     HH"
     gene            complement(2290..3003)
                     /locus_tag="ACZPD3_01360"
     CDS             complement(2290..3003)
                     /locus_tag="ACZPD3_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR1414691.1"
                     /translation="MEMLSLEKELKENSYPGRGIVIGRSADGKKAVTAYFIMGRSENS
                     RNRVFVEDGEGIRTQAFDPSKLTDPSLIIYAPVRVLGNKTIVTNGDQTDTIYEGMDHQ
                     LTFEQSLRSREFEPDGPNYTPRISGVMHVENGKFNYAMSILKSNNGNPDACNRYTFAY
                     ENAIAGEGHFIHTYKCDGNPLPSFEGEPKLVAIPDDMDEFAELLWNSLNEDNKVSLFV
                     RYIDIETGKYESKIINKNK"
     regulatory      complement(3075..3155)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(3247..3951)
                     /locus_tag="ACZPD3_01365"
     CDS             complement(3247..3951)
                     /locus_tag="ACZPD3_01365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR1414692.1"
                     /translation="MSLMLLVIIVLSGWIIINRASENGWSIGDFLHNSDTMVSDGEQS
                     GTDNTDSSGGPEQWQKEGAPYIDVELLTPNEYSRPQIPIESVQYIAIHYTANPGATAI
                     ANRNYFENLATTHDTKVSSHFVVGLDGEVVQCIPTSEMSYATNSRNVDTLSIECCHPD
                     ETGKFNEATYDSAVKLSAWLCVRFGLTSENVIRHYDVTGKNCPKYYVENPDAWIQMKS
                     DIAAQIDVDYGLQDVQ"
     gene            complement(4198..5655)
                     /gene="guaB"
                     /locus_tag="ACZPD3_01370"
     CDS             complement(4198..5655)
                     /gene="guaB"
                     /locus_tag="ACZPD3_01370"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR1414693.1"
                     /translation="MGTIIGEGITFDDVLLVPAYSKVIPNQVDVTTYLTKKVKLNIPM
                     MSAGMDTVTEHRMAIAMARQGGIGIIHKNMSIEAQAEEVDKVKRSENGVITDPFYLSP
                     EHTLKDADELMAKFRISGVPITEGRKLVGIITNRDLKFETDFSKKIKECMTSEGLITA
                     KEGITLEDAKKILAKSRKEKLPIVDDDFNLKGLITIKDIEKQIKYPLAAKDEQGRLLC
                     GAAVGITANVLARVDALVKASVDVIVIDSAHGHSENILKAVREIKAAYPELQVIAGNV
                     ATGAATKALIEAGVDAVKVGIGPGSICTTRVVAGIGVPQITAVMDCYEAAKEYGIPII
                     ADGGIKYSGDVTKAIAAGANVCMMGSMFAGCDESPGTFELYQGRKYKVYRGMGSIAAM
                     ENGSKDRYFQENAKKLVPEGVEGRVAYKGHVEDTVFQLMGGLRSGMGYCGAETIEKLK
                     ETGRFIKISAASLKESHPHDIHITKEAPNYSVDDK"
     gene            complement(5765..6268)
                     /locus_tag="ACZPD3_01375"
     CDS             complement(5765..6268)
                     /locus_tag="ACZPD3_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6106 family protein"
                     /protein_id="HIR1414694.1"
                     /translation="MGDLYSELLVKKDKTAKDSLLKYGLIVLTVLAVFAGLIITPLAL
                     IIAVALGIACYFVIPKTDVEYEYLFINGDFDIDMIMSKTKRKKVKSFKLSEADLAAPL
                     DSHRMDYYNGNQNMKVLDFSSGNPEHKRFGVITRLDGNLCKIILEPDEALAQAMKNSA
                     PSKVFLD"
     gene            complement(6639..8258)
                     /gene="groL"
                     /locus_tag="ACZPD3_01380"
     CDS             complement(6639..8258)
                     /gene="groL"
                     /locus_tag="ACZPD3_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267873.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR1414695.1"
                     /translation="MAKEIKFGAEARAALEAGVNKLADTVRVTLGPKGRNVVLDKPYG
                     APLITNDGVTIAKEIELEDGFENMGAQLIKEVASKTNDVAGDGTTTATVLAQAMVHEG
                     MRNLAAGANPIILRKGMKKATDVAVEAIKNMSQTISGKKQIANVASISASDETVGQLV
                     ADAMEKVSKDGVITVEESKTMHTELDLVEGMQFDRGYVSAYMSTDMEKMEANLEDPYI
                     LITDKKISNIQEILPLLEQIVKVGAKLLIIAEDVEGEALTTLIVNKLRGTFQVVAVKA
                     PGYGDRRKEMLQDIAILTGGQVISEEVGLELKDATMEQLGRAKSVKVAKENTVIVDGM
                     GDKDAIANRVAQIRGQIEETKSEFDKEKLQERLAKLAGGVAVIRVGASTETEMKEAKL
                     RLEDALAATRAAVEEGIIAGGGSAYIHASKEVAKLATTLEGDEKTGANIILKALEAPL
                     FRISANAGLEGSVIINKVRESEPGIGFDALNERYVDMVSEGILDPAKVTRSALQNATS
                     VASTLLTTESAVAIIKEDTPAPAANPGMGMM"
     gene            complement(8404..8688)
                     /gene="groES"
                     /locus_tag="ACZPD3_01385"
     CDS             complement(8404..8688)
                     /gene="groES"
                     /locus_tag="ACZPD3_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR1414696.1"
                     /translation="MTLVPLGDRVVLKQVEAEETTKSGIVLPGQAQEKPQQAEVVAVG
                     PGGVVDGKEVKMEVAVGDKVIYSKYSGTEVKMDGTEYIIVKQNDILAIVK"
     gene            complement(9244..10029)
                     /gene="codY"
                     /locus_tag="ACZPD3_01390"
     CDS             complement(9244..10029)
                     /gene="codY"
                     /locus_tag="ACZPD3_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIR1414697.1"
                     /translation="MSVQLLDRTRKINKLLHNSSSSKVVFNDICQVLMETLSSNILVL
                     SKKGKVLGVSLCPGVEEITELIEDKVGGYVDSLLNERFLGVLSTKENVNLQTLGFEHV
                     SSSYQGIINPIDIAGERLGTVFMYRYEKPYDIEDIIVSEYGTTVVGLEMMRAVHEENA
                     EEDRKQQVVKSAFNTLSFSELEAIIHIFDELDGDEGILVASKIADRVGITRSVIVNAL
                     RKFESAGVIESRSSGMKGTYIKVLNEVIFDELEEIKAQRNKKS"
     gene            complement(10401..>10622)
                     /locus_tag="ACZPD3_01395"
     CDS             complement(10401..>10622)
                     /locus_tag="ACZPD3_01395"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase family protein"
                     /protein_id="HIR1414698.1"
                     /translation="ACPKCGKEIVLKKTKKGRKYYGCEDNPDCDFMSWQKPSAKKCPK
                     CGSYMVEKGNKLVCSQETCGYVEAKDEKE"
BASE COUNT         2773 a         2497 c         1833 g         3519 t
ORIGIN      
        1 ttgcttctat tatttctata tgctatagta aaattatcag aattctattc tgccttacga
       61 aagataagat aaaatataaa tattttttcg taaaggagtg tgattttatg actgctgtta
      121 aagaacgtat tataggtgct gtatcaatta tgagtgataa agatgctaat atcttctggc
      181 atattattca aaaacatttt aaactgcctg atacattcgc agatatagaa aaagtagaac
      241 ccgatgaaac agatttaata atgttaaagg aaatcgaaaa caaccctgac tgtcacgaat
      301 tcatttctca ggaagaatta atgaaagaac ttaatatgta agtattgttc cctgaaaagt
      361 gccaatttca gcacttttca gggagtcata atttttcaat aaaaccatta tataaaattt
      421 caaaataaat aaaaaaacat ttgacaaact ccactgcata tagtatcata tattttgtcg
      481 ctgaggtaat cactcaactg acatcataaa tgcgatagtg gcgtagttgg taacgcgcga
      541 ccttgccaag gtcgagaccg cgggttcgag ccccgtctat cgctcttaaa gcatcagcta
      601 aggctgatgt ttttttcatc cccttttatt aactttccat aactatagca attccatatc
      661 tgcgaataac atctatagcc aatatccttg attaacatcc ctgagtcaag catatattcg
      721 caatccgctt cgctacgcgg ctctacacca gcgcaggaca ttatatgttt ttgcccgcat
      781 ttgcaggagt cttagaaaaa gttagcgcca ggaaatctgc gttatgtaaa aaaatttcac
      841 tgtctgagcc gcatttgcgg cgagtttgaa atttttttac atgtttttag cagatttcct
      901 ggcgcttagt ttttcttagg ctccgaaacc gcggacaaaa acatataatg tcctgcgcgt
      961 ccccatacca gcaccttagt gatggaacaa tctgatacca gtaaaactca tcaccattcc
     1021 atatttattg caagtctcga tcacagcatc atcacgcaca gatccacccg gctgcgcaat
     1081 atacttcacg ccactcttat gagcacgctc aatattatca ctgaacggga agaatgcatc
     1141 agatcccagt gtaacatccg tcatctgatc cagccatgca cgtttctctt cctttgtaaa
     1201 tacttccggt ttctctgtga aagttttctc ccacatacca tcagcaagta catccatata
     1261 ttcatccccg atataaacat caatggcatt gtcacgttcc gcacgtgaaa ttgtatcttt
     1321 aaatggcaga ttcagcactt tcgggcactg gcgaaggaac cagttgtctg ccttctgtcc
     1381 tgcaagtctt gtacaatgta cacgtgactg ctgtcctgca ccaacaccga tagcctgtcc
     1441 atcttttacg aaacatacag agttggactg tgtatatttc agaatgatca gagaaatctt
     1501 catatcacgt tttgcttcct ctgtaagctc tttattctct gtcacaatat tggaaagcat
     1561 tgcatcatcg atctcaagct cctgacggcc ctgctcaaaa gttacaccaa atacttcttt
     1621 gtgttccagc ggagccggta caaagttctc atcaatctgg atcacattgt agttaccttt
     1681 tttcttctgc ttcaggatct ccagtgcttc ctctgtgtat ccaggtgcaa taacaccgtc
     1741 agatacctct ctcttgatca gaagtgctgt atctttatca cacacatcag aaagagaaat
     1801 aaaatcacca aaagaggaca ttctgtcagc accgcgagct cttgcataag cgcatgctaa
     1861 tggtgaaaaa ttcttccagt ccatatcatt tacccagtag atctttgcca gagtctctgt
     1921 aagaggaagt cctacagcag caccggcagg agatacatgt ttaaaagatg tagctgcagg
     1981 aagtcctgtt gctttcttca gattggatac caactgccag ccattaaaag catccaggaa
     2041 attgatatat cccggtcttc cggaaaggat ctttaccgga agctcagatc cgtctgccat
     2101 ataaattttt gcaggtttct gattcgggtt acagccatat tttaattcga attctttcat
     2161 tatataaatc ctccgtatga accaaataag tattttaaaa ttaattcctg taaataattt
     2221 cttactgcga aagcatcgtc ttatgttcac aataatctat gagcactatc tgtactcagt
     2281 aagtgactat tatttattct tattaatgat cttgctttcg tacttacctg tctcaatatc
     2341 aatatatctt acaaacagag atactttgtt atcttcgttc aggctgttcc agagaagttc
     2401 tgcaaactca tccatatcat ccggaattgc taccaatttc ggttcgcctt caaagcttgg
     2461 aagtggattt ccgtcacatt tataagtatg gatgaaatgt ccctctcctg caatggcatt
     2521 ctcgtatgca aatgtgtatc tgttacatgc atccggattt ccgttgttgc tcttgagaat
     2581 tgacattgca tagttgaatt ttccgttctc tacgtgcata acaccggaga tacgaggtgt
     2641 atagttcgga ccgtccggct cgaattctct gcttcttaag gactgctcaa aagtaagctg
     2701 atgatccatt ccctcataaa tggtatctgt ctgatctccg ttagttacga ttgttttgtt
     2761 tcccagtaca cgtacaggtg catagatgat cagactcgga tctgtaagtt tggacggatc
     2821 aaatgcctgg gtacggattc cctcaccatc ctctacaaat actctgtttc tgctgttctc
     2881 gcttctgccc atgatgaaat aagctgtcac tgctttctta ccgtctgcgg aacgtccaat
     2941 cacaattcct ctgccaggat aggaattctc tttcagttct ttttccagtg ataacatttc
     3001 cataattttt ctccttttcg ttaaaaataa ggttaaaaaa agcctgggaa acctatttat
     3061 tcattgaata atttccaggc tgcccaggcg gtcggctcat atactcgctg cactccctgt
     3121 aggtaatcct acttccgcca gtcatgcagg cactgttgtg aaaaaaaatc acacgttgtt
     3181 attatagtac aatacttctt tgtggatttc aaggcaaatt tttttgtact gtgctgaatt
     3241 ttcgttttac tgaacatcct gcaaaccata atcaacatca atctgcgccg caatatccga
     3301 tttcatctgt atccatgcat ccggattctc cacataatat ttcggacagt tttttccggt
     3361 aacatcataa tgccggatca cattctctga tgtaagtcca aaccgcacgc acaaccacgc
     3421 agaaagcttc actgccgaat cataagttgc ctcattgaat ttccccgtct catccggatg
     3481 acagcactcg atggacaaag tatctacatt acgtgagttc gtcgcatagg acatctctga
     3541 cgtaggaata cactgcacca cttccccatc cagccccact acaaaatgac tgctcacctt
     3601 tgtgtcatga gtagtcgcca gattctcgaa gtagttcctg tttgcaatgg ctgttgcccc
     3661 cggatttgca gtataatgaa tggcaatata ctgtacactc tcaatgggaa tctgcggtct
     3721 ggaatattca ttcggcgtca gcagttccac atctatgtac ggcgcccctt ctttttgcca
     3781 ttgttccggt ccgcctgaag aatccgtatt gtctgttcct gactgttccc catcagaaac
     3841 catcgtgtca gaattatgta aaaagtcccc aatactccat ccattttcac tggcccgatt
     3901 tattatgatc cacccactca gaactatgat cacgagcagc atgaggctca taagaattct
     3961 ctgatttctc tgccttactc tgctgctctt tcgtcttttc gacctgctgt ttttcttttt
     4021 tactgcagtt ttccctgttc tggtatctgt ttttttcttt ttgtttatat gcttttgcat
     4081 ttttaccttc tctttcggga ttttactccc tgcaaaaaat accccggccg gcatgcctgg
     4141 aaatcttatt tctttccatc agcagccagc cggggcatat attacacctg aaatttatta
     4201 tttgtcatct acgctatagt tcggagcttc ttttgtgata tgaatatcat gtggatgaga
     4261 ttccttaaga gatgcagcag aaatcttgat gaatctgcct gtttctttaa gcttctcaat
     4321 agtttctgct ccacaatatc ccataccgga acgaagaccg cccatgagct ggaatacagt
     4381 atcctcaaca tgtcctttgt aagcaacacg tccttctacg ccttccggaa caagtttctt
     4441 cgcattctcc tggaagtaac ggtctttact tccgttctcc atagctgcga tagatcccat
     4501 accgcggtat actttatatt ttctgccctg atacagttcg aatgttcccg ggctctcgtc
     4561 gcatcctgca aacatgcttc ccatcatgca gacattcgca ccggctgcga ttgcctttgt
     4621 gacatctccg gaatacttga taccaccgtc tgcaataatc gggattccat attcttttgc
     4681 agcttcgtag cagtccataa cagcagtgat ctgcggaaca ccgatacctg caacaactcg
     4741 tgtggtacag atagatccag gtccgatacc aaccttaact gcatctacac cggcctcgat
     4801 cagagccttt gtcgcagcac ctgttgctac gttacctgcg attacctgca gttccggata
     4861 tgctgccttg atctcgcgta cagccttcag aatgttctct gaatgtccat gtgcagaatc
     4921 aattacgatc acgtctacag atgcctttac aagtgcatca acacgtgcaa gtacattagc
     4981 agtgattcct accgcagcac cgcagagaag acgtccctgc tcatcctttg cagccagagg
     5041 atatttgatc tgtttctcaa tatctttaat agtaattaag ccttttaagt taaagtcatc
     5101 atctacaatg ggtaatttct ctttacgtga ttttgccaga atcttcttgg catcttccag
     5161 tgtaattccc tctttggctg taatcagtcc ctcagaagtc atgcactctt tgatcttctt
     5221 gctgaaatct gtctcaaact tcagatcacg attggtaatg attccgacca gttttctgcc
     5281 ctctgtgatc ggcacaccgg agatacggaa ctttgccatt aactcatctg cgtccttaag
     5341 tgtatgttca ggagataaat agaaaggatc tgtgataaca ccgttctcag aacgttttac
     5401 cttgtctacc tcttctgcct gcgcttcaat ggacatattc ttatgaataa taccaatacc
     5461 accctgtctc gccatagcga tcgccatgcg atgctcggtt actgtatcca ttcctgcact
     5521 catcatagga atgttcagtt taaccttctt tgttaagtaa gttgtgacat ctacctgatt
     5581 cggaattacc tttgaatatg ccggaactaa cagcacgtca tcaaaagtaa tgccttcacc
     5641 aatgatagta cccattgcat ttcctccctt gcgtatatga aattaaatta ctattgtaat
     5701 gtaaaaagga accagcaact ggtcctttta ttttcaacaa gtgtaacaaa aatcagattc
     5761 tatgtcaatc caaaaaaact ttgctcggtg ctgaattttt catagcctgt gcaagtgcct
     5821 catccggttc caggatgatc ttgcataaat ttccgtccag tcttgtgata acaccaaagc
     5881 gtttatgctc cggatttcct gatgaaaagt ccagtacttt catgttctgg tttccgttgt
     5941 aatagtccat tctgtgggaa tccagcggtg ctgccagatc tgcttctgat aatttgaagc
     6001 tctttacttt ctttcttttt gtctttgaca tgatcatgtc gatgtcaaaa tcgccattga
     6061 taaacagata ttcatattct acatctgttt tcgggattac aaaatagcat gcaattccaa
     6121 gcgctacggc aatgatcagt gccagcggtg tgatgataag tccggcaaat actgccagca
     6181 ctgtcaaaac gatcagaccg tatttgagca ggctgtcttt agccgtctta tctttcttta
     6241 ccagtaattc tgaatacaag tcgcccattg tttcgtctcc ttttcatttg ttcagctgtt
     6301 gaaagcagct tttttttgca cagcatattt aacaattata ttttattcat catgctgttt
     6361 ttctgcatta tatcacaaaa cggaaccatc gtctatgacg acggttccgt ttcacatatt
     6421 tccgcctgcg ctgtttttat ctatagcaat cctcgtaaat aatgcattta agacaattca
     6481 gcagagtgcg aaagacaagg aagatatctg caggcgtgct gacgcacggc aaagatatct
     6541 gacgcaggat ttcacactct gatgggctgg attaagatgc attatttcag agaattgcta
     6601 taagcatact acggtataat tttatcgttt attaaaaatt acatcattcc cattccagga
     6661 ttagcagccg gtgcaggtgt gtcttctttg ataattgcaa cagcagattc tgttgtaagt
     6721 aatgtagacg caacgcttgt agcattctga agagcacttc ttgtaacctt tgcaggatca
     6781 aggatacctt cgcttaccat atctacatat ctttcgttca aagcatcaaa gccgattccc
     6841 ggttcggatt ctcttacttt gttgatgatt acggaacctt caagtcctgc atttgcagaa
     6901 atgcggaaca gaggagcttc cagagctttc aggatgatat ttgcacctgt cttctcgtca
     6961 ccttccagag ttgttgcaag ttttgcaact tctttggatg cgtggatgta agcagagccg
     7021 ccacctgcga tgataccttc ttctactgct gctcttgtag ctgcaagagc atcttccaga
     7081 cgaagcttag cttctttcat ttctgtctca gtggaagcac caacacggat aacagctacg
     7141 ccgccagcca gttttgcaag tctttcctgt aatttttctt tatcaaattc agattttgtt
     7201 tcttcaatct gtccacggat ctgagcaaca cggtttgcga ttgcatcttt gtcacccata
     7261 ccgtcaacga taactgtgtt ctcttttgca actttaacag attttgcacg tcctaactgc
     7321 tccatagttg cgtctttcag ttccagacca acttcttcgg agattacctg gccgcctgta
     7381 aggattgcga tatcctgaag catttctttt cttctgtcac catatcccgg agctttgaca
     7441 gctactacct ggaaggttcc tcttaatttg tttacgatca gagttgtaag agcttcaccc
     7501 tctacatctt cagcaatgat aagaagtttc gcaccaactt ttacgatctg ctccagtaat
     7561 ggaaggattt cctggatgtt gctgatcttc ttgtctgtga tcaggatata tggatcttcc
     7621 agatttgctt ccattttttc catatctgtt gacatatatg cggatacata tccacgatcg
     7681 aactgcatac cttctacaag gtcaagttct gtgtgcattg tcttggattc ttctactgtg
     7741 ataacgccgt ctttggaaac tttttccata gcatctgcta ctaactgtcc aacagtctca
     7801 tcgcttgcgg aaatagatgc aacgtttgcg atctgtttct tgccgctgat tgtctggctc
     7861 atgttcttga ttgcttctac agctacatct gtagctttct tcattccttt tctgaggatg
     7921 atcgggttag cgcctgctgc caggtttctc attccttcgt gaaccatagc ctgtgcaaga
     7981 acagtagctg ttgttgtacc atcacctgct acatcgtttg ttttggaagc aacttctttg
     8041 atcagctgtg cgcccatgtt ttcaaatccg tcttccagtt caatttcttt tgcgattgta
     8101 acaccgtcgt ttgtgataag cggagcaccg tatggtttgt caagaactac gtttcttcct
     8161 ttcggtccta atgttactct tactgtatca gctaatttat ttacaccagc ttcaagagct
     8221 gctctggctt ctgcgccgaa tttaatttct tttgccatga taatatgacc tcctgattca
     8281 aattttgatc tctatgaatt ctttctgata cttatccggt atttcatgtt acatcctgta
     8341 aatattatcc tgtaaatatt atcttataag tatcttcatt tatgttttct attttgatat
     8401 tgcttattta acaattgcaa gaatatcatt ctgttttaca atgatatatt ctgtgccatc
     8461 catttttact tctgttcctg aatatttgga gtagataact ttatcaccta cagctacttc
     8521 catttttact tctttgccgt caacaactcc gccaggtcct acagctacaa cttctgcctg
     8581 ctgtggtttc tcctgtgcct gtcccggaag aacgataccg gattttgttg tttcttctgc
     8641 ttcaacctgt tttaatacaa ctctgtcacc taaaggtact aatgtcatga tctatatcct
     8701 cctttattct ccaactaaag ctttcctgtt accgtactct ttccattatt gcaaaatatc
     8761 cggtaactgt ttcggcactc tgtctaatcc tgccgatttt tgctagcact catttacttc
     8821 gagtgctaat cactgtcctt atattattga aaatgagtcg atttcgcaag tgcttttatc
     8881 attataaaat aatatatatt ctctttttta cttatatata ctcttctttt cttttgtttt
     8941 cttaatttac aaataatttt gtcataacta gtacatcgaa aattaagttt ctgctatatt
     9001 gacgcaggat gattgcttca gctgcaaggc ggaggaatga ggcgtagcgg tggctacggc
     9061 gattgacgac aacgccgcag atgaaaaatc agacaagtca atatagcagt tacttaatat
     9121 tcgctgtact agtccggagg gctttattat gttataagca tattacggaa taattttcta
     9181 taacataaca aaaagaccgt ccaccataca gcagacagcc ttcttgtatt cattatttac
     9241 ggatcaggat ttcttatttc tctgagcttt aatctcttcc agttcatcaa agatgacttc
     9301 attcagcact ttaatgtagg ttcccttcat accggatgat ctggattcga tcacaccggc
     9361 actttcaaac ttcctcaaag cgtttacgat aacagatctg gtgattccca cgcggtctgc
     9421 gatcttactt gcaaccagga ttccctcatc tccgtcaagt tcatcaaaga tatggatgat
     9481 tgcctccagt tcggagaaag ataaagtatt aaacgcagat ttcactacct gctgctttct
     9541 gtcctcctca gcattctctt catggaccgc tctcatcatc tccagcccta caacggttgt
     9601 tccatattca ctgacaataa tatcttcaat gtcatatggt ttctcataac gatacataaa
     9661 tacagtaccc agtcgttccc ctgcaatatc tatcggatta atgattccct gataactgct
     9721 tgatacatgt tcaaatccca gtgtctgaag atttacattc tcttttgttg acaggacacc
     9781 cagaaatctc tcgtttaaca gggaatctac atatccgcca accttgtcct cgatcagttc
     9841 agtgatctcc tctactcctg ggcataaact gacacccaga acctttcctt ttttactgag
     9901 aaccagaatg ttggaactca gtgtctccat cagaacctga cagatgtcat taaataccac
     9961 tttgctggaa ctgctgttat gcagaagttt attaattttt cttgttctat ccagcagctg
    10021 aacactcata ttgtcctcct ttcaccctgt acatatccga aatctttctg tcattatttc
    10081 ataaatgagc agtcactttc ttttgctcgc aaggaagatt cagacacaca tggtcattaa
    10141 cattactgat actttgattt tacacgtttt aaagtcaaat tacaagacaa attttactat
    10201 ttttttattt atttttgctt ttctgaaaat agattccttt tataatatta ttcaataaat
    10261 atcatcatca aaaattcaaa ggattgaaga caaaaagtca gaaagtcact gactgtaaga
    10321 caaaaaacgg aactgttgca ttaatgaaat tcattttcaa ctgctgcaac agtcccattt
    10381 ataacattta caatccgtta ttattctttc tcgtcctttg cctctacata tccacatgtc
    10441 tcctggctgc agacaagctt attccccttt tccaccatat agcttccaca tttcgggcat
    10501 ttctttgctg atggtttctg ccaggacatg aaatcacaat ccggattatc ctcacatccg
    10561 tagtacttac gtcctttttt ggttttcttt agcacaatct cttttccaca tttcggacat
    10621 gc
//
LOCUS       DBMDXG010000021        10615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1747_length_10615_cov_1.782766, whole genome shotgun sequence.
ACCESSION   DBMDXG010000021 DBMDXG010000000
VERSION     DBMDXG010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10615
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1747_length_10615_cov_1.782766"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(21..1295)
                     /gene="purD"
                     /locus_tag="ACZPD3_01400"
     CDS             complement(21..1295)
                     /gene="purD"
                     /locus_tag="ACZPD3_01400"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR1414699.1"
                     /translation="MKVLIVGSGGREHAIAWSVSKSPKVDKIYCAPGNAGIAELAECV
                     DIGAMEFEKLADFAQEKEIDLTIIGMDDPLVGGVVDVFEARGLKVFGPRKNAAILEGS
                     KAFSKDLMKKYNIPTAAYENFDDPEKALEYLRTEARFPIVLKADGLALGKGVLICKDL
                     KEAEDGVKEIMEDKKFGNAGNTMVIEEFMTGREVSVLSFVDGKTIRTMTSAQDHKRAQ
                     DGDQGLNTGGMGTFSPSPFYTEEVDEFCRKYVYQPTVDAMAAEGRPFKGIIFFGLMLT
                     PDGPKVLEYNARFGDPEAQVVLPRMKNDIIEVMEACVNGTLDQIDLQFEDNAAVCVVL
                     ASDGYPVKYEKGIPMYGFENFKGKDGYYCFHAGTKLKDGQIVTNGGRVLGITATGKDL
                     KEARKNAYEATEWITFANKYMRHDIGKAIDEE"
     gene            complement(1321..1962)
                     /gene="purN"
                     /locus_tag="ACZPD3_01405"
     CDS             complement(1321..1962)
                     /gene="purN"
                     /locus_tag="ACZPD3_01405"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR1414700.1"
                     /translation="MLKVGVLVSGGGTNLQAILDAIDCGKITNAEVSLVISNNPKAYA
                     LERAKNHNIEAVCISPKQYESREEFHKTLLEKLKESGVELIVLAGFLVAIPPMIVEAY
                     PNKIINIHPSLIPSFCGVGYYGLHVHEKALARGVRVTGATVHFVDTGTDTGPIILQKA
                     VKIKSDDTPEVLQRRVMEKAEWKILPKAINLIANGKVKVVDGRVEIEEYDTEE"
     gene            complement(1956..2981)
                     /gene="purM"
                     /locus_tag="ACZPD3_01410"
     CDS             complement(1956..2981)
                     /gene="purM"
                     /locus_tag="ACZPD3_01410"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR1414701.1"
                     /translation="MDYKTAGVDIEAGYKSVELMKEHVKRTMREEVLGGLGGFSGAFS
                     LAKIKEMEEPVLLSGTDGCGTKVKLAMVMDKHDTIGIDAVAMCVNDIACAGGEPLFFL
                     DYIACGKNYPEKIAAIVGGVAEGCVQSDAALIGGETAEHPGLMPEDEYDLAGFAVGVC
                     DKKEMITGEELKAGDVLIGMASTGVHSNGFSLVRKVFDMTKESLDTYYEELGTTLGEA
                     LLAPTRIYVKALKSIKNAGVRVHACSHITGGGFYENIPRMLKEGTRAVVEKNSYPVLP
                     IFKLLAEKGNIDEQMMYNTYNMGLGMILAVDAADVDKTMEAIKAAGDTPYVVGRIEDG
                     EKGVTLC"
     gene            complement(3031..3531)
                     /gene="purE"
                     /locus_tag="ACZPD3_01415"
     CDS             complement(3031..3531)
                     /gene="purE"
                     /locus_tag="ACZPD3_01415"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR1414702.1"
                     /translation="MAKVGIVMGSDSDMPIMSKAADILEKLGIDYEIKIISAHREPDV
                     FFEYAKTAEEKGFKVIIAGAGMAAHLPGMCAAIFPMPVIGIPMHTTSLGGRDSLYSIV
                     QMPSGIPVATVAINGGANAGLLAAKILATSDEELLAKLKAYSQELKEQVEAKDARLQE
                     VGYKNY"
     gene            complement(3765..5783)
                     /locus_tag="ACZPD3_01420"
     CDS             complement(3765..5783)
                     /locus_tag="ACZPD3_01420"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR1414703.1"
                     /translation="MERYELAKERIAQIPDEKMAEEPYCDFFTKEAMFLQQVIHVMDH
                     GIAGKNLEELQEQNHELYQDILPENYENSYGNPAYAQKMLGEYGKVFTFLYTELHGTI
                     AYAFEKKAWDLTVVLELFLEIYSAFTQEEIPAEKQVKEILVSYVNDYCQDMVENRIRE
                     AIDPENNFVVEMIMDSDLSDLSYLYQSGEYVSENELKTAEFMNSLSQREIDEMARTYT
                     EGYRMGFITGRKDITKKKTVNIRYHLGFERMVKAAVLQFREMGLQTVIYRHALHAVNR
                     RNQFRNGFTGGIANPQFDYDHRQDSALFLNPDFVKRKLRAMQTSYDEYADLADVHGGP
                     AVIETFGEKPFSPVSKPESWAFTEAQQKLQLELDNESGQITNRYIKGEERSFTIIAYP
                     IPEIGEDFPEIFREIVKINTLDYKKYQKIQQTIIDTLDTCEWVEIKGKGENETDLLIH
                     LHTLTDAKTQTNFENCVADVNIPLGEVFTSPVLAGTGGMLHVSKVYLNGLQFCDLKLV
                     FDCGQVIDYSCSNFETEEENRKYIEDNILFHHPKLAMGEFAIGTNTTAYVAAQKYNIA
                     DKLPILIAEKMGPHFAVGDTCYSWSEDTPVYNPDGKEIIARDNEISEMRKEDVSLAYY
                     GCHTDITIPYDELGSIRTIDDEGDMISIIEDGRFVLPGTEALNEPFGE"
     gene            complement(5837..6157)
                     /locus_tag="ACZPD3_01425"
     CDS             complement(5837..6157)
                     /locus_tag="ACZPD3_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6142 family protein"
                     /protein_id="HIR1414704.1"
                     /translation="MAKRYRYAFAKKREAQQGKLSAVLAAVSLVLFVTAVLLAFFLQG
                     QYGYIVGGISLCAMLLSVYGFAMGLKSFSEENRTHKAGMIGSIANGIIMIGWLGIFLM
                     GISG"
     gene            complement(6141..6614)
                     /locus_tag="ACZPD3_01430"
     CDS             complement(6141..6614)
                     /locus_tag="ACZPD3_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1414705.1"
                     /translation="MKKETKHIIRTLGTILFILYVLALIYFLFFSEEYGRAAMEERQY
                     RYNLIPFVEIRRFWVYRKQLGLMAVVTNLFGNVIGFLPFGFILPVILDKMRSGWLIVL
                     AGFGLSVTVEVIQLITKVGCFDVDDMILNTAGAALGYLLFFICDHLRRKIYGKKI"
     gene            complement(6640..6792)
                     /locus_tag="ACZPD3_01435"
     CDS             complement(6640..6792)
                     /locus_tag="ACZPD3_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414706.1"
                     /translation="MNEKIYKTMSSTGACSLTVGIIVLATGIVTGIMMIVNGARLLKK
                     KSEILI"
     gene            complement(6871..7551)
                     /gene="pyrE"
                     /locus_tag="ACZPD3_01440"
     CDS             complement(6871..7551)
                     /gene="pyrE"
                     /locus_tag="ACZPD3_01440"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR1414707.1"
                     /translation="MEQYKQEFIEFMVDSEVLKFGEFTLKSGRKSPFFMNAGGYVTGS
                     QLKKLGEYYAHAIHDKYGDDFDVLFGPAYKGIPLSVVTAIAFSELYGKEIRYCSDRKE
                     EKDHGADKGSFLGSKLKDGDRVVMIEDVTTSGKSMEETVPKVKGAADVEIVGLMVSLD
                     RMEVGKGGVKCALDEVHDLYGFETNAIVTMREVIEHLYNKEYKGKVIIDDTLKAAIDA
                     YYEQYGAK"
     gene            complement(7578..8480)
                     /locus_tag="ACZPD3_01445"
     CDS             complement(7578..8480)
                     /locus_tag="ACZPD3_01445"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR1414708.1"
                     /translation="MDMSVNIAGVEWKNPVTVASGTFGSGAEFADYVDINKLGAVTTK
                     GVANVPWVGNPTPRVAEVYGGMMNAIGLQNPGIDLFCERDIPFLKQYDTKIIVNVCGH
                     APEEYLAVVERLADQPIDMMEINISCPNVNAGFLAFGQDAHHVEELTAQIKKIAKQPV
                     IMKLTPNVTDITEIAKGAEAGGADAVSLINTLTGMKIDINRKTFALANKTGGVSGPIV
                     KPIAVRMVYQVAQAVNIPIIGMGGISCAEDAIEFILAGASAVSVGTANFHNPAVTLEV
                     IDGIEAYMKKNGFNSVKEMVGIVK"
     gene            complement(8480..9268)
                     /locus_tag="ACZPD3_01450"
     CDS             complement(8480..9268)
                     /locus_tag="ACZPD3_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR1414709.1"
                     /translation="MSEKTREICTVVSQESIGAGIYSMWIQTDRIAADAKPGQFVSLY
                     TNDKSKILPRPISLCEIDKENGRLHLVYRVTGQGTGTDEFSQMKAGDTIPVLGPLGNG
                     FPVEKAEGKKVFLMGGGIGVPPILELAKQMKCEKKQIIAGYRDCHTFLREEFEAAGTL
                     YIATEDGSVGTKGNVMDAIRENALEADVIYACGPTPMLRAIKKYAEENGIECYISLEE
                     RMACGIGACLACVCKSREKDAHSNVNNKRICKDGPVFLSTEVEI"
     gene            complement(9279..10205)
                     /gene="pyrF"
                     /locus_tag="ACZPD3_01455"
     CDS             complement(9279..10205)
                     /gene="pyrF"
                     /locus_tag="ACZPD3_01455"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR1414710.1"
                     /translation="MINKLIENIKKTNAPIVVGLDPMLNYIPQHIQKKAFSELGETLE
                     GAAEAIWQYNKGIVDAISDLIPAVKPQIAMYEQFGIPGLMAYKNTIDYCKEKGLVVIG
                     DIKRGDIGSTSAAYAVGHLGKVQVGSNRIAGFDEDFATINPYMGSDSVNPFIDVCKEE
                     NKGLFVLVKTSNPSSGEFQDRIIDGRPLYEWVGEKVAQWGESHMGNEYSYVGAVVGAT
                     YPEMGKTLRKIMPKTFILVPGYGAQGGKGADLVHFFNEDGLGAIVNSSRGIIAAYKQE
                     KYASFGELNYADASRQAVKDMIEDISTALNNR"
     gene            complement(10239..>10615)
                     /locus_tag="ACZPD3_01460"
     CDS             complement(10239..>10615)
                     /locus_tag="ACZPD3_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1414711.1"
                     /translation="ISTDHAPHAVPEKTGSIRNAAFGIVGIETSFALSYTALVETGIL
                     TISQLIEKMSWNPAQILGSDRGTLQKGHPADIVIADIDHEYKIDKNEFASKGRNTPFD
                     GWKVKGKILYTICDGKVVYQNA"
BASE COUNT         2701 a         2700 c         1816 g         3398 t
ORIGIN      
        1 tttttaaaca tcaatatttt ttattcttca tcaattgctt ttccaatatc atggcgcata
       61 tatttatttg cgaatgtaat ccactctgtg gcttcatagg cattttttct tgcttccttc
      121 aggtcttttc ctgtagctgt gattcccagg acacgacctc cgttggttac gatctgacca
      181 tcttttaatt tggttcctgc atggaaacaa taataaccat ccttaccttt gaagttctca
      241 aatccgtaca tcggaatacc tttttcatat ttcacaggat atccgtcact tgcaaggact
      301 acacatactg ccgcattatc ctcaaactga agatctatct gatccagagt tccgtttaca
      361 caggcttcca tcacttcgat gatatcgttc ttcatacgag gaagaacaac ctgcgcttcc
      421 ggatcgccaa aacgggcatt atattccaga accttcggac catccggtgt aagcataaga
      481 ccaaagaaaa tgattccctt aaatgggcgt ccttctgcag ccattgcgtc cactgtcggc
      541 tgatatacat atttcctgca gaattcatct acctcttctg tgtagaacgg actcggggag
      601 aaagttccca ttccgcccgt attcaatccc tgatcaccgt cctgagcgcg tttatgatcc
      661 tgtgcggatg tcattgtacg gatagttttt ccatctacaa atgataacac agagacttca
      721 cgtcctgtca taaattcttc gatgaccatg gtatttccgg catttccgaa tttcttatct
      781 tccatgatct cttttacgcc gtcttctgcc tctttcaaat ccttgcagat cagcactccc
      841 ttgccgagtg caagaccatc tgctttcaga acaattggaa atctggcttc tgtgcggaga
      901 tattccagtg ctttctcagg atcatcaaaa ttctcatatg ctgctgtcgg gatattatat
      961 ttcttcatta aatctttcga gaaagcttta gatccctcaa ggatcgcagc gttctttctt
     1021 ggtccgaata ctttcagacc gcgggcctca aatacatcta cgacaccacc gaccagcgga
     1081 tcatccatgc caatgatcgt aagatcgatt tctttttcct gtgcaaaatc agcaagtttc
     1141 tcaaattcca ttgcaccgat atctacacat tctgcaagct ctgcgattcc tgcatttcca
     1201 ggtgcacaat aaattttatc tactttcggg ctttttgaaa cactccatgc aatcgcatgt
     1261 tctctgccgc cgctgcctac aattaagact ttcattttat cttgccgctc ctttttgtat
     1321 ctactcttcc gtatcgtatt cttctatttc cactcttcca tccacaacct tcaccttgcc
     1381 attggcaatg agattgattg ccttaggaag aattttccac tctgctttct ccatgacacg
     1441 tctctggaga acttccggag tgtcatcaga tttaattttc actgctttct gaagaataat
     1501 cggaccggta tctgtgccag tatcaacgaa atgtaccgta gcgcctgtaa cacgaacccc
     1561 tcttgctaac gctttctcat gcacatgaag tccgtaataa ccaactccgc agaaagacgg
     1621 aatcagtgac ggatggatat taatgatctt atttggatat gcttccacga tcatcggcgg
     1681 gattgctacg aggaatcctg ccagaacgat aagctctacg ccactctctt tcagcttttc
     1741 cagaagtgtt ttgtgaaatt cttctctgct ttcatactgt tttggagaaa tacatacagc
     1801 ttctatgttg tgattctttg ctctctccag cgcataagcc ttcggattat tgctgataac
     1861 cagagaaact tctgcatttg taatctttcc acagtcaatg gcatccagga tagcctgcag
     1921 attcgtgccg cctcctgaaa ccagtacacc gacttttaac ataaagtcac tcccttttca
     1981 ccatcttcga tacgtcctac tacgtacgga gtgtcacctg ctgctttgat cgcttccatt
     2041 gttttgtcca catctgctgc atctacagca aggatcattc caagtcccat gttataagta
     2101 ttgtacatca tctgttcatc aatattgcct ttttcagcaa gaagtttgaa gattggaagt
     2161 accggatagc tgttcttttc cacaactgca cgtgttccct ctttaagcat tctcggaata
     2221 ttttcataaa aaccgccgcc tgtgatatga ctgcatgcgt gcacacgaac tccggcattt
     2281 ttaatgctct ttaatgcttt tacatagatt ctggttggtg ccagaagtgc ttcaccaagt
     2341 gttgttccaa gttcttcata ataagtatca agagattcct ttgtcatatc aaaaacttta
     2401 cggacaagag aaaatccgtt actgtgaaca cctgtggatg ccataccgat caaaacatct
     2461 cctgctttca gttcttcacc tgtgatcatc tcttttttat cgcagacacc tactgcgaaa
     2521 ccagccaggt catattcgtc ttccggcatc agtcccggat gctctgctgt ctcaccaccg
     2581 atcagagctg catctgactg cacacagcct tctgcaacgc cgcctacgat agctgcaatc
     2641 ttttccggat aattcttacc acatgcaata taatcaagga agaataatgg ttctccgcct
     2701 gcacatgcaa tatcattgac acacatggca actgcatcaa tgccaatggt gtcatgcttg
     2761 tccataacca tagcaagttt tactttagtt ccacatccat ctgtacctga taataatacc
     2821 ggttcttcca tttctttgat cttagcaaga gaaaaggcac cggaaaatcc tcccagtcct
     2881 cccagcactt cttcacgcat ggttctcttt acatgctctt tcatcagctc tactgattta
     2941 tatcctgctt caatatctac tccagcggtc ttgtaatcca tgataatcct ccgtttttac
     3001 ccctgatcct gtttatggac ctgagttatc ttagtaattt ttatatccga cttcctgtaa
     3061 tcttgcgtct tttgcttcca cctgttcttt taattcctgg ctgtaagcct ttaattttgc
     3121 aagaagttcc tcatctgatg ttgcaagtat cttagctgcc agaagtcctg cgtttgcgcc
     3181 tccgttgata gcgactgttg caaccgggat tcctgacggc atctgtacga tagaataaag
     3241 ggaatctctt cctcccagag aagtggtgtg cataggaatt ccgataaccg gcatcgggaa
     3301 gattgctgcg cacattcccg gcagatgagc tgccattcct gcacctgcaa tgattacttt
     3361 aaagcctttt tcctctgctg tttttgcata ttcaaaaaag acatccggtt ccctgtgtgc
     3421 ggaaatgatc tttatctcgt aatcaattcc cagtttctcc agaatgtctg ctgctttgct
     3481 catgatcggc atatctgagt cgctgcccat cacaattcct acttttgcca tgattattct
     3541 ccttttcttc tcaagtattc tcagacagac cagattttat ctgtccggga ctttgttttt
     3601 attttattga taaaaaaagt acggtcagaa aagattttat caaaaccctc tctaaccgta
     3661 gattctacta agattccctt tttctgtcaa taaaattaat gcaaagaaat attttaacga
     3721 actaataaat ttttttgtat tccgaactta ataggaaaat gcctttattc cccaaacggc
     3781 tcgttcagag cttccgtacc cggaaggaca aatcttccat cttcaataat agagatcata
     3841 tccccctcat catcaatcgt acggatgctt cccagttcgt catatgggat cgtaatatct
     3901 gtgtgacatc cataataagc aaggctgacg tcttcttttc gcatctcgga aatctcattg
     3961 tcccttgcga tgatctcttt accatccgga ttatagaccg gagtatcttc tgaccagctg
     4021 taacaggtat caccaactgc aaaatgcggt cccattttct ccgcgatcag aatcggaagt
     4081 ttgtctgcga tattatattt ctgcgctgcc acataagctg ttgtattagt tccgattgca
     4141 aattctccca ttgcaagttt cggatgatgg aacagaatat tatcttcaat atatttacgg
     4201 ttctcttcct ctgtctcaaa attagaacag ctgtaatcga tcacctgtcc gcagtcaaac
     4261 acaagcttca ggtcacaaaa ctgcagtccg ttaagatata cttttgaaac atgaagcatt
     4321 ccgcctgttc ctgcaagcac cggtgaagta aatacttcac ccagaggaat atttacatct
     4381 gccacacagt tctcaaaatt tgtctgtgtt ttcgcatctg ttaatgtatg aagatgaatg
     4441 agcagatctg tctcattctc tccctttccc ttgatttcca cccattcaca ggtatcaaga
     4501 gtatctatga tcgtctgctg gatcttctga tatttcttat aatccaatgt attgatcttt
     4561 acgatctcgc ggaaaatttc cggaaaatcc tctccgatct caggtatcgg ataagcaatg
     4621 atcgtaaaac ttctttcctc acctttgata taacgatttg tgatttgacc ggattcattg
     4681 tccagttcca gctgaagctt ctgctgtgct tctgtaaacg cccagctttc cggtttactt
     4741 accggtgaaa acggcttctc tccaaatgtc tcgatcacag ccggtccgcc atgtacatct
     4801 gccagatctg catattcatc ataagaagtc tgcatggcac gaagtttacg ctttacaaaa
     4861 tccggattca gaaacagcgc actgtcctgt ctgtggtcat aatcaaactg cggatttgca
     4921 attcctcctg taaatccatt tctgaactga ttccgtctgt tcaccgcatg aagtgcatgg
     4981 cggtaaataa ccgtctgcag ccccatctcc cggaactgaa gaacagccgc ttttaccata
     5041 cgctcaaagc caagatgata acgtatatta accgttttct tctttgtaat atcttttctt
     5101 cctgtgatga atcccatccg atagccttct gtataagtac gtgccatctc atcaatttcc
     5161 ctctgggaaa gtgaattcat aaattctgct gttttcagtt cattttccga tacatattcg
     5221 ccggactgat acagataact cagatcgctc agatcagaat ccatgatcat ttcaaccaca
     5281 aaattattct caggatcaat cgcctctctg atccgatttt ccaccatatc ctggcagtag
     5341 tcattcacat aggaaacgag aatttctttc acctgtttct cagcaggaat ttcttcctgc
     5401 gtaaatgcag aatagatttc cagaaacaat tcaagtacaa ccgtaagatc ccatgctttt
     5461 ttttcaaacg cataagcaat cgtgccatga agctctgtat aaagaaaggt aaatactttt
     5521 ccgtattctc ccagcatttt ctgtgcatat gcaggatttc cataagaatt ttcataattt
     5581 tcgggcagaa tatcctgata taattcatga ttctgttcct gaagttcttc aagatttttc
     5641 cccgcaattc catggtccat cacatgaatc acctgctgta aaaacattgc ttcctttgta
     5701 aaaaaatcgc agtacggttc ttctgccatt ttctcgtccg gtatctgagc gattctttct
     5761 ttcgccagtt cataccgttc cataacccat tcgtccaaac tatatttctc ctttttctat
     5821 cagacttccc tgttaatcag ccggaaattc ccatcagaaa gattcccagc catccgatca
     5881 ttatgattcc gtttgcaata gagccgatca tacctgcttt gtgtgtccgg ttttcttctg
     5941 aaaagctttt cagtcccatc gcaaatccat atacagacag aagcattgcg caaagactga
     6001 tcccgcctac aatatatccg tattgtccct gcaaaaaaaa tgccagcaaa actgcagtca
     6061 caaataaaac cagtgaaaca gccgccagca ctgctgacag ttttccctgc tgtgcctctc
     6121 ttttctttgc aaacgcatat ctatatcttt ttgccataaa tttttctcct caggtgatcg
     6181 cagataaaaa aaagcagata tccaagagcc gcccctgctg tattcaatat catatcatct
     6241 acgtcaaagc acccaacctt tgtaataagc tggatgacct ctaccgtaac actaagccca
     6301 aatcctgcca gtacgatcag ccatccgctt ctcattttgt ccagaataac cggcagtata
     6361 aatccaaatg gaagaaaacc aattacattt ccaaaaagat ttgtaactac cgccataagt
     6421 ccaagctgtt ttctgtatac ccagaaacgc cttatctcaa caaacggtat cagattatat
     6481 ctgtactgtc tttcttccat tgctgctctt ccgtattctt ctgaaaagaa cagaaaataa
     6541 atcagtgcca gaacatacag gataaacaaa atggttccca gtgtccgtat gatatgcttc
     6601 gtttcttttt tcaatgtttt ctcctgtttc tgaagccttt tatatcaata tctctgattt
     6661 ctttttcagc agtcttgcac cgtttacgat catcatgatc cctgttacga ttcccgttgc
     6721 cagtacaatg atcccgactg tcagactgca ggctcctgtg gaagacatgg ttttataaat
     6781 tttttcgttc ataatcgtcc tcatcagtat catgatctgc tttcgcagat catgatatct
     6841 tatttataaa tcgctgttac ctgtatttgc ttattttgca ccgtactgct cataatatgc
     6901 gtcgattgcg gctttcaatg tatcgtcaat gattactttt cctttatatt ctttattata
     6961 aagatgttca atgacctctc tcattgtaac aatcgcattt gtttcaaacc cataaaggtc
     7021 atggacttcg tcaagggcac acttcacgcc gccttttccg acttccatgc ggtccagaga
     7081 aaccataaga ccgacgattt ccacatcagc cgcacctttt accttcggta ctgtctcttc
     7141 catggattta ccggatgttg ttacatcttc gatcatcact acacgatcgc cgtcttttaa
     7201 tttacttccg aggaagcttc ccttatctgc accatggtct ttttcttcct tacggtctga
     7261 acagtagcgg atttcttttc catataattc gctgaatgca attgcagtta caacacttaa
     7321 cgggattccc ttatatgcag gtccgaaaag cacatcgaaa tcatcgccat atttatcatg
     7381 aatggcatgt gcgtagtact cacccagttt ctttaactga gaacctgtca catatccgcc
     7441 tgcattcata aaaaacgggg actttcttcc gcttttaagg gtgaattctc cgaatttcag
     7501 aacttcactg tctaccataa attcaataaa ttcctgttta tactgttcca tcttatgtta
     7561 tgcctcctgt atctgtttta ttttacaata ccaaccatct cttttacgga attgaatcca
     7621 ttcttcttca tgtacgcttc aattccatcg atcacttcca gtgtcactgc cggattgtgg
     7681 aaatttgccg tacccacaga tactgcgctg gctcctgcaa gaataaactc aattgcatcc
     7741 tctgcgcagg aaattccgcc cattccgatg atcgggatat tgactgcctg tgcaacctga
     7801 taaaccatac ggacagcaat cggtttcacg atcggtccgg aaacaccacc ggttttattt
     7861 gcaagcgcaa aagtcttcct gttgatatca atcttcattc ctgtcagtgt attgatcagg
     7921 gataccgcat ctgcaccgcc tgcttccgca ccttttgcaa tctctgtgat atctgtgaca
     7981 ttcggtgtta acttcatgat cacaggctgt tttgcaatct ttttgatctg agcggtaagt
     8041 tcctctacat gatgtgcatc ctgaccaaat gcaaggaatc ctgcgtttac attcgggcag
     8101 gaaatattaa tctccatcat atcaattggc tgatctgcca aacgttccac cactgccaga
     8161 tattcttccg gcgcatgacc gcatacattt acaatgatct ttgtatcata ctgtttcaaa
     8221 aaaggaatat ccctctcaca gaaaagatcg ataccaggat tctgcagacc aatcgcattc
     8281 atcatgcctc cgtaaacttc agccacacgc ggagtcggat ttccgaccca cgggacattg
     8341 gctacgccct tggttgtcac tgctcccagc ttattaatat ctacataatc agcaaattct
     8401 gctcctgaac caaaggttcc ggatgccact gttaccggat ttttccactc tacgccggca
     8461 atatttacac tcatatccat cagatttcca cctccgtaga aagaaatacc gggccatcct
     8521 tgcagattct cttattattc acattgctat gtgcatcttt ttctctggat ttgcacacac
     8581 aggcaaggca ggcaccgatt ccgcatgcca tacgttcctc cagggaaata taacattcaa
     8641 tcccattttc ttcggcatat ttcttgattg cacgaagcat cggagttggt ccgcaggcat
     8701 agatcacgtc cgcttccaga gcattctctc tgatagcatc cataacattg ccttttgttc
     8761 ccacactgcc atcctctgtg gcaatataaa gtgttcccgc cgcctcaaat tcctctctga
     8821 ggaacgtatg acagtccctg tatcctgcaa tgatctgttt tttctcacat ttcatctgct
     8881 ttgcaagttc caggatcgga ggaacaccga ttccaccacc cataaggaaa acttttttcc
     8941 cctcagcctt ctctacagga aaaccatttc ccagaggtcc cagtaccgga atggtatctc
     9001 cggctttcat ctgtgaaaat tcatcggttc cggttccctg tccggtcact ctgtaaacca
     9061 gatgaagacg gccattttct ttgtcaatct cacaaagact gatcggtctg ggaagaattt
     9121 tggatttatc atttgtataa agagatacaa actgtcctgg tttcgcatct gctgcgattc
     9181 tgtcagtctg gatccacata ctgtaaatgc ctgcaccaat gctctcctga cttacaacag
     9241 tgcagatttc tcttgtcttt tcgctcattt catgcctcct agcgattgtt taaagcagta
     9301 ctgatgtctt cgatcatatc ttttaccgcc tggcgggatg catctgcata atttaattca
     9361 ccaaaggaag catatttttc ctgtttgtaa gctgcaatga ttcctctgga agagttgacg
     9421 atcgcaccaa gtccgtcttc attaaagaaa tgaacaagat cggcaccttt tccgccctgt
     9481 gcaccatatc ccggtaccag gatgaaggtt ttcggcatga tcttacggag agtttttccc
     9541 atttccggat aggttgcacc tactacagca cctacatagc tgtattcatt tcccatatgt
     9601 gattctcccc actgggcaac cttctctcca acccactcat aaagcggacg tccgtcaatg
     9661 attctgtcct gaaactctcc gctggacgga ttggatgttt ttacgagtac aaaaagccct
     9721 ttgttctctt ctttacatac atcgataaac gggttgacac tgtcagatcc catatatgga
     9781 ttgattgtcg caaaatcttc gtcaaaacct gcgatcctgt tacttcccac ctgaacttta
     9841 cccagatgtc cgactgcata tgcagcagat gtggaaccaa tgtcacctcg tttgatatct
     9901 ccaataacta ccagaccttt ttctttgcaa taatcaatgg tgttcttata tgccattaag
     9961 cccggaatac caaactgctc atacattgcg atctgtggtt tcactgcagg gatcagatcg
    10021 ctgatcgcat caacgattcc tttattatac tgccagatag cttctgctgc tccctctaat
    10081 gtttccccca gttcagaaaa agctttcttc tgaatatgct gcgggatata gttgagcatc
    10141 ggatccagtc ctactacgat aggagcgttt gtttttttaa tgttttcaat aagtttgttg
    10201 atcatgagtc tttcctcctg aatattgata tggtattttt atgcattctg gtatacaact
    10261 tttccatcac agattgtata gagaattttt ccctttactt tccatccgtc aaatggtgtg
    10321 ttgcgtcctt tggaagcaaa ttcattttta tctattttat attcgtgatc aatgtctgca
    10381 atgacaatgt ctgccggatg tcccttctga agtgtacctc tgtcacttcc cagaatctgt
    10441 gctgggttcc agctcatttt ctcaataagc tgtgagattg taagaattcc agtttctaca
    10501 agtgcagtat aagacaatgc aaagcttgtc tcaattccca caatgccaaa agccgcattc
    10561 cggatagagc ctgtcttctc cggcactgca tgaggtgcat gatctgtact gatca
//
LOCUS       DBMDXG010000022        10510 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1762_length_10510_cov_2.101739, whole genome shotgun sequence.
ACCESSION   DBMDXG010000022 DBMDXG010000000
VERSION     DBMDXG010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1762_length_10510_cov_2.101739"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            237..1478
                     /gene="thiC"
                     /locus_tag="ACZPD3_01465"
                     /pseudo
     CDS             237..1478
                     /gene="thiC"
                     /locus_tag="ACZPD3_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            complement(1538..2113)
                     /locus_tag="ACZPD3_01470"
     CDS             complement(1538..2113)
                     /locus_tag="ACZPD3_01470"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1414712.1"
                     /translation="MQKNPYENVIAVTNRSLCQRPFAEQIERVCSLHPKAVILREKDL
                     PEEEYSRLAEQILEICKRYQVPCILHTYVNVAEKLHHPYIHLPIFLLEKYEGKLGGFR
                     QIGSSVHSVEDALKAESLGADYLTAGHIYTTDCKKGLPPRGLEFLENVCKAVKIPVYA
                     IGGIHPGTGQLNEIMEHGSAGGCIMSDMMKI"
     gene            complement(2115..3356)
                     /gene="thiH"
                     /locus_tag="ACZPD3_01475"
     CDS             complement(2115..3356)
                     /gene="thiH"
                     /locus_tag="ACZPD3_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HIR1414713.1"
                     /translation="METTEKNMEHGEHFNESIVNEVILEDMKKNRIDHMKYLPGMEVL
                     EESDVMDQVISAMNAYDYDKYTEADVRRALAHDNRTPEDFQALLSPAALPLLEEIAQA
                     AQKETRKHFGNSVYMFTPIYIANYCENYCIYCGFNCHNKIRRAKLNAEEIDKEMAAIA
                     KTGLQEILILTGESRAKSDVKYIGEACKIARKYFKVIGLEVYPMNSDEYAHLHECGAD
                     YVTVFQETYNSDKYETLHLAGHKRIFPYRLNAQERALKGGMRGVGFAALLGLDDFRKD
                     AFATGYHAYLLQRKYPHAEIAFSCPRLRPIINNDRINPMDVHEPQLLQVVCAYRLFMP
                     FASITVSTRECERVRDNLVGIAATKISAGVSTGIGSHVEDIEDKGDDQFEISDGRSVD
                     EVYKALLDHDLQPVMNDYVYL"
     gene            complement(3623..4402)
                     /locus_tag="ACZPD3_01480"
     CDS             complement(3623..4402)
                     /locus_tag="ACZPD3_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR1414714.1"
                     /translation="MSTNNEDKLILGGHEFTSRFILGSGKFSLDLVKACIEKADAQII
                     TLALRRANEGGLANILDYIPKNVTLLPNTSGARNAEEAVRIARLSREIGCGDFVKIEV
                     IHDSKYLLPDNYETVKATEILAKEGFVVMPYMYPDLNAARDLVNAGAACIMPLGSPIG
                     SNKGLCTKEFIQILIDEIELPIIVDAGIGRPSQACEAMEMGASAVMANTAIATAGDVQ
                     IMAEAFKKAIEAGRSAYLAGLGRTLEKGASASSPLTGFLHE"
     gene            complement(4454..4657)
                     /gene="thiS"
                     /locus_tag="ACZPD3_01485"
     CDS             complement(4454..4657)
                     /gene="thiS"
                     /locus_tag="ACZPD3_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR1414715.1"
                     /translation="MLITVNGKTKELQNTLTIGQYLEENSYVPSQIAVELNERILSKA
                     EYSTTVLHENDILEIVSFMGGGR"
     regulatory      complement(4911..5019)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5065..6210)
                     /locus_tag="ACZPD3_01490"
     CDS             complement(5065..6210)
                     /locus_tag="ACZPD3_01490"
                     /inference="COORDINATES: protein motif:HMM:NF039407.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HIR1414716.1"
                     /translation="MMLCSIREEIPKNQQKRPVALMIPVNLRNYFPSQSMTNFFGWIE
                     VGYIFSDETTFEDVLLSVKKQFEEELVKEKIAMHMSGYVRIEKNPFVRAVPLEIKKYF
                     LMIGANLGSRSITAVYSNIGIIRLPEEYKEYIQHFGIFASTNSLQMCSCSYGDEMVLG
                     FTSKIPDDSIQRNFQRMLGEENVSHRELKNEFPGYGEKHRLEKKENQKVIQTFSFLCL
                     AIAVICGMINFMMAGVLNWFWFAGAGCACAWLVVMVAYYKRRNILKNEMWQLLLISVI
                     AILWDRFTGWKGWSVDFVIPFGILAVQFSVPVIAKINRLEREEYLFYLVQAGIAGLIP
                     MILVWTGIVQFAVPSVICAGISFLTLAALFIFCKKDTMREFHKKLRM"
     gene            complement(6216..6377)
                     /locus_tag="ACZPD3_01495"
     CDS             complement(6216..6377)
                     /locus_tag="ACZPD3_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414717.1"
                     /translation="MHFLQELVQDYLILAHPQADLPQIEHAEEITHGDKEEISTGKPA
                     PVEFPSLFY"
     gene            complement(6405..7136)
                     /locus_tag="ACZPD3_01500"
     CDS             complement(6405..7136)
                     /locus_tag="ACZPD3_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1414718.1"
                     /translation="MRLLIVRHGDPDYSIDSLTEKGWKEAEYLSERLSKLDVKDFYVS
                     PLGRAKDTASFTLKKMNRTAVECDWLREFDVLIDRPDVTDRQKRLWDWLPQDWTQDEK
                     FYQYDHWYENERLQQSDAKGYYDYVTGKFDKLLAEHGYVREGHYYRVEKPNEDTLVFF
                     CHFGLECVLLAHLIGASPMVLWHGFCAAPSSVTTVNTEERREGIASFRISAFGDISHL
                     YVHDEPPAFAARFCEMYSNTDERHD"
     gene            complement(7528..8232)
                     /locus_tag="ACZPD3_01505"
     CDS             complement(7528..8232)
                     /locus_tag="ACZPD3_01505"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047465 - N-acylglucosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HIR1414719.1"
                     /translation="MITKKEILDAIHGKMIISCQAVEDEPLYVEEKSIMYLMARAAKQ
                     AGTPAIRTSSIRDVIAIKEETGLPVIGLVKIQYPGYEGYITPTMKEVDDLVAAGSDVV
                     ALDCTLRKRGDGITVNEFIAQIKEKYPDIILMADISNYEEGINAWKCGVDIVGTTMSG
                     YTDYTSKKDEPDYELMERLAKDTDIPIIGEGKIHYPDQAVKALQTGVWAIVVGGAITR
                     PLEIAQRFQKAIEAGK"
     gene            complement(8318..9421)
                     /gene="nagA"
                     /locus_tag="ACZPD3_01510"
     CDS             complement(8318..9421)
                     /gene="nagA"
                     /locus_tag="ACZPD3_01510"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1414720.1"
                     /translation="MIIKNGKVFQEDGSYKVTDLYVENGRIVASADEVTDKTQLDASG
                     LKILPGLVDIHSHGAVRHDFSDADVDGLRTILQYEKSQGITSYCPTSMTLPKEELLKI
                     FQTAKDVEQDETCARIVGINMEGPFLDPVKKGAHVEGYIRKPDIEFFRACNEAAGGMI
                     KLVTLAPNMEGSEEFIRELHNEVVISIGHTAADYGCAAEAMKEGALHVTHLYNAMNSM
                     GHREPGVIGAAADNQDCMVEMIGDGIHIHPVTVRNTFRLFGDSRVVLISDSMMATGME
                     NGLYELGGQEVTMKDRKATLADGTIAGSATCLFDCMKCVISMGVPEREAILAATANPA
                     RSIGIYNEVGSLTPGKRADIVLTDEELNIVKVL"
     gene            complement(9533..10276)
                     /gene="nagB"
                     /locus_tag="ACZPD3_01515"
     CDS             complement(9533..10276)
                     /gene="nagB"
                     /locus_tag="ACZPD3_01515"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR1414721.1"
                     /translation="MIIYVGKDYQDVSRKAANIMSAQIIMKPNAVLGLATGSTPVGLY
                     KQLIEWYNKGDLDFSQITSVNLDEYKGLSGDNDQSYRYFMNTNLFDHVNIDKNKTYVP
                     NGLEEDSDKACADYNEIIRSVGGIDIQLLGIGGNGHIGFNEPGEAFEKETHCVDLTES
                     TIKANARFFESMDEVPKQAYTMGIKNIMAAKKILLVATGSAKADALYKSLYGPITPNV
                     PASILQLHQDVTVVADEDALSLIKEKGLL"
BASE COUNT         2820 a         2485 c         1905 g         3300 t
ORIGIN      
        1 ctggtcgatg taacggtcca gtattttcat aatcttaact ctcctttata catatatgta
       61 cacgaagcct gatgtaaggc gtccttgtaa aaaatggaag tgttaacgtt tgcatacaaa
      121 acagtagtaa tttagctcaa ttaaatccgg tatataacta tgaattgcgt gcattttgtc
      181 ttttaaatcc ttaaaatcca tttttttaat cttgaatcca tcttaacaca aaaaaagcag
      241 aaaaagaaca ctttgacgtg gaagaattaa gagaactgat tgcaaaagga caggtaatta
      301 ttccctgcaa caaaaatcac aagagcatca gtccgagcgg tgtcggtgca aagctgacca
      361 ccaagatcaa tgtaaattta ggtgtttcca gagactggaa agacgttgat atggaatacg
      421 aaaaggtaca ttcagcagta gaaatgggtg cagaagccat catggatctc agctcctacg
      481 gagataccag aagcttccgc cgcaaactta catccgaatg tccggcaatg atcggtactg
      541 ttccgatcta cgacgcagta gtatattatc acaaagcact tggcaagatt acttctgaag
      601 agtggattga tatcgtacgt atgcacgcag aagacggcgt ggattttatg actatccact
      661 gcggtatgaa tcgtgcaacc gcagcacgtt tcaaacagaa taaacgtctc atgaacattg
      721 tttccagagg tggttctatt atgttcgcat ggatggaaat gacaggaaaa gagaacccgt
      781 tctatgaaca ttttgatgaa attcttgata tctgcagaga gtatgatatc actttaagtc
      841 tgggagatgc atgtcgtccc ggatgtattg cagatgcaac tgatactgca cagattgaag
      901 agctgatcac tctcggtgag cttaccaaac gtgcatggga aaaggatgtt caggttatga
      961 tcgagggacc tggccacatg ccgctgaacc agatcgcagc taatatggag atccagaaaa
     1021 ctctttgcca tggcgctcct ttctatgtac ttggaccgct tgtaacagat gttgctcccg
     1081 gatatgacca cattacttcc gcaatcggcg gtgcgattgc tgcttattca ggtgctgctt
     1141 tcctctgcta tgtaacacct gcagaacatc ttcgtctgcc aaatgcagct gatgtaaaag
     1201 agggaattat tgctgccaaa attgctgctc atgctgctga tattgccaaa ggagttcccg
     1261 gtgccgcaga atgggattat aagatgagcg aagcgagaaa acgtcttgac tgggaagaaa
     1321 tgttcaaact cagcatggat cctgagaaag caagacgcta ccgcgcagag gccaaacctg
     1381 agaaagaaga cacctgcagt atgtgcggaa acttctgtgc cgtaaagaat acaaaccgta
     1441 ttctggatgg tgaaattgtt actatttttg atgaatgatg cttttataaa agccgtattt
     1501 caacatgttt gattgttgaa atacggcttt ttatttacta tatcttcatc atatctgaca
     1561 taatacatcc accggctgaa ccatgttcca taatctcatt taactgtcct gttcccggat
     1621 gtatccctcc tatggcatat acaggaatct ttactgcttt acagacattc tccagaaatt
     1681 ccagtcctct gggtggaagt ccttttttac agtcagttgt ataaatatgt cctgccgtaa
     1741 gataatcagc tcccagactt tctgctttca atgcatcttc tacggagtgg accgaacttc
     1801 cgatctgccg gaaacctcca agctttccct catatttttc caacagaaat attggcagat
     1861 gaatgtatgg atgatgcagt ttttctgcta catttacata ggtatgtaat atgcagggaa
     1921 cctgatatct cttacagatt tccagtatct gctctgccag acgagaatat tcttcctccg
     1981 gcagatcctt ttccctcaga ataaccgcct tcggatgaag gctgcataca cgctcgatct
     2041 gttctgcaaa gggacgctga cacagggagc ggttggtcac tgcaattaca ttttcataag
     2101 gatttttctg cattttagag gtacacataa tcattcataa ccggctgaag atcatgatcc
     2161 aaaagtgcct tatagacttc atctacagac cttccgtctg aaatctcaaa ctggtcatct
     2221 cctttatctt cgatatcctc cacatgactt ccgattcctg tgctgactcc tgctgaaatc
     2281 tttgtcgcag caattccaac caggttatcg cggacacgct cacactctct ggtagatact
     2341 gtaatgcttg caaatggcat gaacagacga taggcgcaga ccacctgaag cagctgtggt
     2401 tcatgtacat ccataggatt aatacggtca ttgttgatga tcggccgaag tctcggacag
     2461 gaaaatgcaa tctctgcatg aggatatttt ctctgcaaaa gatatgcatg atatccggtg
     2521 gcaaaagcat ccttacggaa atcatccagt cccagaagtg cagcaaatcc tactcctcgc
     2581 ataccaccct ttaaagcacg ttcctgtgcg ttcagacgat aaggaaatat acgcttgtgc
     2641 cctgcaagat gcagagtttc atacttatct gaattataag tctcctggaa taccgttaca
     2701 taatcagctc cgcattcatg gagatgtgca tactcatctg aattcatagg ataaacttca
     2761 agtccgatta ctttgaaata tttccgggca atcttacatg cttctccgat atattttaca
     2821 tcagattttg ctctgctctc tcctgtaaga atcaggattt cctgcagtcc tgtctttgcg
     2881 atagcagcca tttccttatc aatctcttct gcattcagct ttgctctgcg gatcttatta
     2941 tggcagttaa atccgcagta aatacagtaa ttctcacagt aatttgcaat ataaataggg
     3001 gtaaacatat acacactgtt cccaaaatgt ttcctggttt ctttctgcgc agcctgtgcg
     3061 atttcctcca gaagaggcaa tgcagccgga gacagcagtg cctgaaaatc ctctggtgta
     3121 cggttatcat gagcaagtgc cctgcgtaca tctgcctctg tatatttatc ataatcgtaa
     3181 gcattcatgg cagaaataac ctgatccatc acgtctgatt cttccagtac ttccattccc
     3241 ggaagatatt tcatatgatc gatcctgttc tttttcatat cttccagaat cacttcattg
     3301 acaatacttt cgttgaagtg ttccccatgt tccatatttt tttctgttgt ttccattgat
     3361 gttttctcct ctttattacc atttggcaac tttcgttttc ctataaatta atacctactc
     3421 cgcagttata gcaatcctct aaaataatgc atcttaatcc agcccatcag agtgtgaaat
     3481 cctgcgtcag atatctttgc cgtgcgtcag cacgcctgca gatatcttcc ttgtctttcg
     3541 cacgctgctg aactgtctta aatgcattat ttacgaggat tgctataatc aaaaatctca
     3601 ttttagaatg attttttatt tattactcat gaagaaaacc agtcagtgga gaggatgcgc
     3661 ttgcaccttt ttccagggtt cttcccagtc cggcaaggta tgcacttcgt cctgcttcaa
     3721 tagctttttt aaatgcttca gccattatct gcacatcacc tgctgtggca atcgcagtat
     3781 ttgccataac tgcagatgcc cccatctcca tggcttcaca cgcctgagaa ggacgtccga
     3841 ttcctgcatc tacaatgatc gggagctcaa tttcgtcaat caaaatctgg ataaattcct
     3901 tagtgcacag acctttatta gagccgatcg gagaacccag cggcatgatg caggcagctc
     3961 cggcatttac cagatctctg gctgcattca gatccgggta catataaggc attactacaa
     4021 atccttcttt tgcaagaatt tctgtagctt taacagtttc atagttatcc ggaagcagat
     4081 attttgaatc atggatcact tcgatcttta cgaaatcacc gcatccgatt tctctggaaa
     4141 gtctggcaat acgaactgct tcttctgcat ttctggcacc tgaagtattg ggaagaagag
     4201 taacattttt cggaatatag tccagaatat ttgccagtcc accttcattg gcacgacgca
     4261 gagccagagt gatgatctgc gcatctgcct tttcaataca tgctttcacc agatccagag
     4321 aaaattttcc ggatcccaga ataaaacggg aagtaaattc atgacctccc agaatcagtt
     4381 tgtcttcatt atttgtactc ataatttaag tccttctttc gtatattata atgtgatatc
     4441 aggatattcc tgtttatctt ccacctccca tgaagctgac tatttccagt atgtcatttt
     4501 catgtaaaac agtagtgctg tactcggctt ttgacagaat tctttcattt aattctactg
     4561 cgatctggct gggcacatag ctgttttcct ccagatattg ccctattgta agagtattct
     4621 gcaattcttt tgtttttcca ttaacagtaa taagcatact ttctcctttc cgggaaaaga
     4681 aggtccggcc tctacaacat aaaaaagagt ccacaaaaga atacacccgc ggtggtgcac
     4741 cccttcatga actctgttca ctctcatctt tcccaatatt catctgttat aatgtaaatt
     4801 tccctgtaag cccccggttt tctctcactt attacagatt gattaccgga cagctgaatt
     4861 tcttcaaatg catgagataa ataaaaagga gatgccagtc atctcccttt aaaatcatat
     4921 cttataggtt cctacgttgg cattatccaa atcaggtata tgggtcgaag ttaatgactt
     4981 cctctcagcc tgcactcaca agctcccctt tctttacatg ggtaaagtat aatcttttat
     5041 cccatgtgtg tcaagaccat atttctacat cctcagtttc ttatgaaact cccgcatcgt
     5101 atccttcttg cagaagataa acagtgcagc caatgtcaga aaactgattc cggcacagat
     5161 tactgaaggt acggcaaact gcacgattcc agtccaaacc aatatcatcg gaatcagtcc
     5221 tgcaattccg gcctgtacaa gataaaacag atattcttcc ctttccagac ggtttatttt
     5281 tgcaatcaca ggaacagaaa actgcactgc caatatcccg aaaggaatca caaaatccac
     5341 agaccagccc ttccatccgg taaaccggtc ccataggatt gcaattactg atatgagcaa
     5401 aagctgccac atttcattct tcagaatatt cctgcgttta taatatgcta ccataacaac
     5461 cagccatgca caggcacatc ctgctcctgc aaaccagaac cagttcagta cacctgccat
     5521 cataaaatta atcattccgc aaattaccgc aattgccaga cacaggaagc tgaaagtctg
     5581 gataaccttc tgattttctt ttttctcaag cctgtgcttt tccccatatc ccggaaattc
     5641 attctttaat tctctgtggg aaacattctc ctcgccgagc atccgctgga aattcctctg
     5701 gatactgtca tccggtatct tggaagtaaa tcccagtacc atctcatccc cataagaaca
     5761 ggaacacatc tgcaaagaat tggtacttgc aaaaatccca aaatgctgaa tatattcttt
     5821 gtactcctcc ggcaaacgaa taatgccgat attagaataa actgccgtaa tgctcctgct
     5881 tcccagattg gctccgatca tcagaaaata tttcttgatc tccaacggaa cagcacgtac
     5941 aaaaggattc ttctcaatgc gcacatatcc gctcatatgc atggcaatct tttcttttac
     6001 cagttcttct tcgaactgtt tcttcacaga caaaagtaca tcttcaaagg ttgtttcatc
     6061 agaaaatata tatcccacct caatccatcc aaagaaattt gtcatagact gagacggaaa
     6121 ataatttctc agattcaccg gaatcatcaa tgctaccggt cgtttctgct gattctttgg
     6181 aatctcttcc cgaatagaac acagcatcat tgctgtcagt aaaacagtga tggaaactcc
     6241 acaggagcgg gctttccggt ggatatctct tctttatctc catgtgtaat ttcctctgca
     6301 tgctcaatct gcggaagatc tgcctgtgga tgtgccagga tcagataatc ctgaaccagt
     6361 tcctgcagga aatgcatggc tccgcaggtg aattacctag ttaatcaatc gtggcgctca
     6421 tccgtattac tgtacatttc acagaatctt gcagcaaatg ccggtggttc atcatgcaca
     6481 tataaatggg aaatatcacc aaatgcacta atacgaaaac ttgcgattcc ttctcttcgt
     6541 tcttctgtat tgacagttgt cactgaactt ggtgctgcgc agaatccatg ccacagtacc
     6601 attggagaag caccgatcag atgcgcaaga agcacacatt ccagaccaaa atgacagaag
     6661 aataccagcg tatcttcatt tggtttctcc actctgtaat aatgtccttc acgtacataa
     6721 ccatgttcgg caagaagctt atcaaacttt cctgttacat agtcatagta tcctttggca
     6781 tcagactgct gaagtctctc gttttcatac cagtgatcat actggtaaaa tttctcgtcc
     6841 tgtgtccagt cctgcggaag ccagtcccat aatctctttt gcctgtcagt aacatccggg
     6901 cgatcgatca gaacatcaaa ttccctcagc cagtcacact caactgctgt acggttcatt
     6961 ttcttcaatg taaaagaggc tgtatccttt gcccttccaa gaggtgatac atagaaatcc
     7021 tttacatcaa gttttgacaa tctttctgac agatattctg cttctttcca gcctttttct
     7081 gtaagagaat caatggaata atccggatct ccatgtcgca cgatcaataa tctcattcgt
     7141 attgttcctt tcttttgtct cagttcttct attatctcag atcagatcgt atccaaatag
     7201 tctggcatct tccattcata gatcttatct gagtttttta atttccacat tcttctgtaa
     7261 ccggagggat tgtttcctca taaatatcgt tactgttcac tccgttcaca gtaactaaat
     7321 atcttctgat gtggataagt tctgcttatc atatgacgct tctattctaa caccaagttc
     7381 cggtttctgc actaaaaatt ctctgcagtc cggttcagtt gctgcaaaag tcggccagct
     7441 gtttatttat aaccattcca taaaatactg cacttgctat aacaaaaaag gatgtgcaac
     7501 tctgacacat ccttttttgt tcgcatttta tttacctgct tcaattgcct tctgaaatct
     7561 ctgtgcgatt tccagcggac gtgtgatcgc tccgcctacc acgatcgccc atacacctgt
     7621 ctgtagtgct tttacagcct gatccggata atgaatcttg ccttctccga taatcggaat
     7681 atctgtatct tttgcaagcc tctccatcag ttcataatct ggttcatcct tttttgaagt
     7741 gtaatcagtg taaccgctca tggttgttcc aacaatatcc acaccacact tccatgcatt
     7801 gataccttct tcataattag aaatatctgc catcaggata atatccgggt atttctcttt
     7861 gatctgtgcg ataaactcat tgacagttat gccatcacca cgcttgcgaa gtgtacaatc
     7921 cagagctaca acatcagatc ctgctgctac cagatcgtct acttctttca tggtaggtgt
     7981 gatatatcct tcatatccgg gatactggat tttcaccaga ccgatcaccg gaagacctgt
     8041 ttcttctttg atcgctatga catcgcggat actgcttgta cggattgcag gtgtgcctgc
     8101 ctgttttgca gcgcgtgcca tcaggtacat aatggatttc tcttctacat ataatggttc
     8161 atcttctacc gcctgacagg aaatgatcat ttttccatga atcgcatcta aaatctcttt
     8221 tttagttatc atttttctcc ttgatacaga actgcattcc ggcaaaatgt ccatactctt
     8281 atctgtgcac attctgtcgg aaagcttttc ttttacacta cagtacctta acaatattca
     8341 actcttcgtc tgtcaacaca atatctgccc gttttccggg agtaagggaa cccacttcat
     8401 tataaatccc aatacttctc gccggatttg ctgttgctgc cagaatcgct tctctttccg
     8461 gtacacccat ggaaataaca cacttcatac aatcaaaaag gcaggttgca gaaccggcga
     8521 tcgttccgtc tgccagagtt gcctttctgt ctttcatggt cacttcctgt ccgcccagtt
     8581 catacagtcc gttctccata cctgttgcca tcatggagtc actgataagg acgactctgc
     8641 tgtctccaaa taaacgaaag gtattgcgta cagttacagg atggatatgg atcccatcac
     8701 cgatcatctc taccatacag tcctgattat ctgctgctgc accgatcaca ccgggttctc
     8761 tgtgtcccat agagttcatg gcattataaa gatgagttac atgaagtgct ccctctttca
     8821 tcgcttctgc tgcgcagcca taatctgctg ctgtatgtcc gatagaaata accacttcat
     8881 tatgtaattc tctgataaat tcctcagagc cttccatatt cggagcaaga gttaccagtt
     8941 tgatcattcc gcccgcagct tcgttacatg ctctgaaaaa ttcaatatcc ggtttacgga
     9001 tatagccttc tacatgtgct ccctttttca caggatcaag gaaaggccct tccatattga
     9061 tacccacaat acgggcacag gtttcatcct gttctacatc ttttgcagtc tggaaaatct
     9121 taagcagctc ctctttcgga agtgtcatgg atgtcgggca ataggatgtg attccctgag
     9181 atttttcata ctgaaggatt gttcttaacc catcaacatc tgcgtctgag aaatcatgtc
     9241 tgactgcacc atggctgtgg atatctacca gtccgggcag aatcttcagt cctgatgcat
     9301 ccagttgcgt tttgtcggtt acttcatctg ctgaagccac aattctgccg ttctctacat
     9361 acagatctgt gactttataa ctgccatctt cctgaaaaac ttttccgttc tttataatca
     9421 tctgattttt gcctcttttc tcgctgaagt gaattattaa gtatttccct gactttatct
     9481 ctgtcaggca attctttcca ctgatactgt tgttatcaca caagagccgg tattataaaa
     9541 gtcctttttc tttaataaga cttaacgcat cttcatctgc cactacggtt acatcctgat
     9601 gaagctgaag gatagatgca ggtacatttg gtgtgatcgg tccgtacaga gatttgtaca
     9661 aagcatctgc cttggcactt cctgttgcta caagaagaat tttctttgct gccataatgt
     9721 tcttgattcc catggtgtat gcctgtttcg gaacttcatc catactttca aaaaatcttg
     9781 catttgcctt gatagtgctt tctgtaaggt ctacacaatg agtttccttc tcaaaagctt
     9841 ctccgggttc attgaaacca atatgtccgt ttccgccgat tcccagaagc tggatatcaa
     9901 ttccacctac actgcggatg atctcattat aatctgcaca tgctttgtcg ctgtcttctt
     9961 ccagtccgtt tggtacatag gttttgtttt tgtcaatgtt tacatgatcg aacaggtttg
    10021 tgttcatgaa ataacgatag ctctggtcgt tgtcaccgga aagtccttta tactcatcca
    10081 ggtttacaga tgtgatctgt gagaaatcaa ggtcaccttt gttgtaccac tcgataagct
    10141 gtttatataa tccgaccggt gtagagcctg ttgccagtcc aagaactgcg tttggtttca
    10201 taataatctg ggcagacatg atattagctg cttttctgct tacgtcctga taatctttac
    10261 ctacataaat aatcatagtt ttctcctttt ttgcgtgtca tctgacactt ccgatttgtt
    10321 ataaactcat tataattccc gttggttcta ttttcaatga atgtattgtt tttagaatct
    10381 tctttcattt tttattattt tgagccattt tgcagttcgg tttttaaagc tttccgaaat
    10441 tactttcctt gtttatattt ttcatgaaaa tatttacatt ctccttgtga aacaaattct
    10501 acaatgttat
//
LOCUS       DBMDXG010000023        10435 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1775_length_10435_cov_2.069479, whole genome shotgun sequence.
ACCESSION   DBMDXG010000023 DBMDXG010000000
VERSION     DBMDXG010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1775_length_10435_cov_2.069479"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(61..1254)
                     /gene="tuf"
                     /locus_tag="ACZPD3_01520"
     CDS             complement(61..1254)
                     /gene="tuf"
                     /locus_tag="ACZPD3_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR1414722.1"
                     /translation="MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKVLAERVAGNV
                     VENFEDIDKAPEERERGITISTAHVEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGVMAQTKEHVLLARQVGVPYIVVFMNKCDMVDDEELLELVEMEIRELLS
                     EYDFPGDDIPVIKGSALKALEDPNGEWGDKIMELMDAVDSYIPDPQRDTDKPFVMPVE
                     DVFSITGRGTVATGRVEAGVLHVSDEVEIVGIKEETRKVVVTGIEMFRKLLDEAQAGD
                     NIGALLRGVQRNEIERGQVLAKPGTLTCHTKFTAQVYVLTKDEGGRHTPFFNNYRPQF
                     YFRTTDVTGVCNLPEGTEMCMPGDNIEMTIELIHPIAMSQGLTFAIREGGRTVGSGRV
                     ATIIE"
     gene            complement(1590..3710)
                     /gene="fusA"
                     /locus_tag="ACZPD3_01525"
     CDS             complement(1590..3710)
                     /gene="fusA"
                     /locus_tag="ACZPD3_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR1414723.1"
                     /translation="MAKEGREYPLERTRNIGIMAHIDAGKTTLTERILYYTGVNYKIG
                     DTHEGTATMDWMEQEQERGITITSAATTCHWTLEENCKPKPGALEHRINIIDTPGHVD
                     FTVEVERSLRVLDGAVGVFCAKGGVEPQSENVWRQADTYNVPRMAFINKMDILGANFY
                     GAVEQIKTRLGKNAICLQLPIGKEDDFKGIIDLFEMKAYIYNDEKGDDISIVDIPEDM
                     KEDAELYHTELIEKICELDDDLMMEYLEGEEPTVERLKATLRKATCECTAVPVCCGSA
                     YRNKGVQKLLDAILEYMPAPTDIPPIEGTDLDGNPVVRHSSDEEPFAALAFKIMTDPF
                     VGKLAYFRVYSGTMNSGSYVLNATKDKKERVGRILQMHANKRMELDKVYSGDIAAAIG
                     FKFTTTGDTICDEQHPVILESMEFPEPVIELAIEPKTKAGQGKLGEALAKLAEEDPTF
                     RAHTDQETGQTIIAGMGELHLDIIVDRLLREFKVEANVGAPQVAYKETITKAVDVDSK
                     YAKQSGGRGQYGHCKVKFEPMDANGEELYKFESTVVGGAIPKEYIPAVGEGIEEAMKA
                     GILGGFPVVGVYANVYDGSYHEVDSSEMAFHIAGSLAFKDAMQKAAPVLLEPIMKVEV
                     TTPEDYMGDVIGDINSRRGRIEGMEDIGGGKMIRGYVPLSEMFGYATDLRSRTQGRGN
                     YSMFFEKYEQVPKSVQEKILAKKD"
     gene            complement(3728..4198)
                     /gene="rpsG"
                     /locus_tag="ACZPD3_01530"
     CDS             complement(3728..4198)
                     /gene="rpsG"
                     /locus_tag="ACZPD3_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR1414724.1"
                     /translation="MPRKGHTQKRDVLADPMYNNKVVTKLINNIMLDGKKGVAQKIVY
                     GAFARIEEKAGKPALEVFEEAMNNIMPLLEVKARRIGGATYQVPIEVRADRRQALALR
                     WLTMFSRKRGEKTMEERLANELLDAMNNTGASVKRKEDMHKMAEANKAFAHYRF"
     gene            complement(4423..4839)
                     /gene="rpsL"
                     /locus_tag="ACZPD3_01535"
     CDS             complement(4423..4839)
                     /gene="rpsL"
                     /locus_tag="ACZPD3_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR1414725.1"
                     /translation="MPTFNQLVRKGRQTSVKKSTAPALQKTFNSLRKKAVEQSAPQKR
                     GVCTAVKTATPKKPNSALRKIARVRLSNGIEVTSYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGTRYHIIRGTLDTAGVANRKQARSKYGAKRPKKK"
     gene            5623..6636
                     /locus_tag="ACZPD3_01540"
     CDS             5623..6636
                     /locus_tag="ACZPD3_01540"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1414726.1"
                     /translation="MKKPSMISILLFLTGLLCIIALIFFNRTTLHRILTTEKEVDTIS
                     EQITAKHDEVKQAVDKYTTAIDTVQSNINRMKQKQQTATQKKTKEKAEIPEEASSKPS
                     VFSQNSTSASSDSSASVFSSDAETDGHIIGIDPGHQSESVDMSAPEPNGPGSSEMKAK
                     CTSGTQGTYSGVPEYQLNLEVSLQLKDELEQRGYQVVMTRTDNETAISNMERAQYAAA
                     QGAEIYVRIHANGDDSHTASGALTMSPSQNNPYIPQLFEQSDRLSRCIIDSYCAATGF
                     QNLGIQYTDTMTGINWSTVPVTILEMGFMTSQNDDLKMNDAEFQKTMVQGIANGIDSY
                     FAS"
     gene            6753..7853
                     /locus_tag="ACZPD3_01545"
     CDS             6753..7853
                     /locus_tag="ACZPD3_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR1414727.1"
                     /translation="MNKKPNEKPIFLYIVIGILTVAVIALAAVSFLSLNHLVTLQQKV
                     QTLSKTVEEISTDANTLISQADQLDELREQESSVKAATGETSPAQSESAEPSSESEQQ
                     NGTLSPSSNNTFTDNTDSSMDNLLKQVQSLLPADNGTWSVYVCNLPKDSEGMINDTPM
                     QAASLIKLYIMGAVYENYDTLSQSHNGDEIDSNISAMITVSDNDAANTLVNWLGNGDD
                     SAGMAKVNGFCQEHGFTSTQMNRLLLASKENGDNYTSVKDCGTFLKQIYQTVNGTLPA
                     STLPNADAMYYHLKMQQRKNKIPAQLPEGVGTANKTGELDTVENDAAIIYDTAKGIDL
                     VVCFMSQDLTDTGAAQSTIAADARAIYGYYNE"
     gene            complement(8106..9335)
                     /locus_tag="ACZPD3_01550"
     CDS             complement(8106..9335)
                     /locus_tag="ACZPD3_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414728.1"
                     /translation="MKNRMQDLDFEQNVAFDKVQEYEFTRRAAQRFRQVVSLDSFEDE
                     DADVIFHYLYKEMELVSFGDHLKRYIYERAELEEPFSEVPQEVYKEIVVDSFKETYTP
                     KSMNPTSTKLSALVNNWLNQASVKRETVFLLGFGLKMTTEDVSDFLTRVLKEQDFDFH
                     NPDEVIYWYCYSTQQGYHKAEELKKKYEILAPVEVENTQVLYGSNLCLDTEEKLIDYL
                     ARLKSKRVDPISEKSQAFQEFTKLLYHAKQIIAGLYQHDEEEKGGDKVWTAERITPSD
                     VEKVICSGIPINKMGNLKKMSASILAKHFSQKRFSRQRITNILSHKLPVERFDLITLE
                     FFIVSQEMEDDDPFNRYKHFLDEIQDILLRCGMGEIYIVNPYECFLLMCLLTDCPLAV
                     FSEIWEKSYEEGEAEEA"
     gene            complement(9349..10167)
                     /locus_tag="ACZPD3_01555"
     CDS             complement(9349..10167)
                     /locus_tag="ACZPD3_01555"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR1414729.1"
                     /translation="MAMYQIECAYTCHTGNIRANNEDNFWCFGESLPVNNEGTKGICS
                     KIISGNRVPAMAVFDGMGGESCGEIAAFLASEEFGKFYNANKRMLRDMPEDFIDDVCE
                     KMNQAVCRYGTEHHIWSMGSTMAMLLFTPESMFACNLGDSRIYFMDGGKLQQISTDHV
                     FGGTAVGKAPLTQYLGLPEELQRLEPSVTEIEHKEGYRYLLCSDGVTDMLSDSEIEAI
                     LSQDMEIPEIVNDLLKNALQKGGRDNVTIVLCEVQKLDTVRRMKEWMKNLKDKK"
     gene            complement(10148..>10435)
                     /locus_tag="ACZPD3_01560"
     CDS             complement(10148..>10435)
                     /locus_tag="ACZPD3_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR1414730.1"
                     /translation="MKALVYPAESGDGVYEEYFYWGEKLFFAYIWYDETSEYYYYNDG
                     ELIRWIDSNGKCHDNEKDNEEFVKRGEKYWNNSLKALKGEGTKTDNGNVSD"
BASE COUNT         2867 a         2556 c         1945 g         3067 t
ORIGIN      
        1 cggggtatct tacgtttgga cacatgtacc ttgcggtact caatatatat tagtcagtga
       61 ttactcgatg attgtagcaa cacgtcctga tcctacagta cgtccacctt cacggatagc
      121 gaaagtaaga ccctggctca tagcgattgg gtggatcagt tcgattgtca tttcgatgtt
      181 atctccaggc atacacattt ctgttccttc tggaaggttg caaacacctg ttacgtctgt
      241 tgttctgaag tagaactgtg gacgatagtt gttgaagaat ggagtatgac gtccaccttc
      301 atcttttgtc agaacgtaaa cctgagctgt gaattttgta tggcatgtaa gtgttcctgg
      361 tttagcaaga acctgtccac gctcgatttc gtttctctga acaccacgaa gaagtgcacc
      421 gatgttatca ccagcctgag cttcatcaag aagtttacgg aacatctcga taccagttac
      481 aactacttta cgtgtttctt ctttgatacc aacgatttca acttcgtcag atacatgaag
      541 tacaccagcc tctactctac cagtagcaac tgtaccacga cctgtgatag agaatacatc
      601 ctctacaggc attacgaatg gtttatctgt gtcacgctgt gggtctggaa tgtagctgtc
      661 aacagcatcc ataagttcca tgatcttgtc tccccactca ccgttcggat cttcaagagc
      721 tttaagagca gaacctttga ttaccgggat atcatctcct gggaaatcat actcggaaag
      781 aagttcacga atctccatct caaccagttc aagaagttct tcgtcatcaa ccatatcaca
      841 tttgttcatg aatacaacga tgtaaggaac acctacctga cgagcaagaa gaacgtgctc
      901 ttttgtctga gccataacac catctgtagc tgctacaaca aggatagcac catccatctg
      961 agctgcacct gtgatcatgt tcttaacgta gtcagcatgt cctgggcagt caacatgtgc
     1021 ataatgtctc ttctctgtct gatactcaac gtgagctgta gaaattgtga taccacgctc
     1081 tctttcttct ggagctttat cgatatcttc aaagttttct actacgtttc cagcaactct
     1141 ctcagccaga acttttgtga ttgcagctgt taaagttgtt ttaccatggt caacgtgtcc
     1201 aatggtacca atgttacaat gcggttttgt tctctcaaac ttagcttttg ccattttaaa
     1261 acgtcctcct taaaacaatt ggctcttata ttattataga gcatcttttc ggttttaaac
     1321 ttgctatcct tgattatatt gcataatcag aatatttgca agcattttat acaatttgat
     1381 aacttgcatt accaattgtt atatcattac agctcaccca cgccgctatc acacgaggtg
     1441 ttttggcatg tatatgccaa aattccattg cagaaaacaa tctggaatga atttttggca
     1501 acgttactga gaacgaagtg aacaataacg ttatatctcc tgcagtccgt tggctttaaa
     1561 caccaacaga ctgcagatga tcaaaattat cagtctttct tagcaagaat cttttcctgt
     1621 acagacttcg gaacctgctc atatttctca aagaacatag agtagttacc acgtccctgt
     1681 gttctggaac gaaggtctgt agcatatccg aacatttcgg aaagtggaac gtatccacgg
     1741 atcatcttac caccgccgat atcttccata ccttcgatac gaccacggcg ggagttgata
     1801 tcaccgataa catcacccat gtaatcctct ggtgtagtaa cttcgacttt catgataggc
     1861 tcaagaagaa ccggagctgc tttctgcata gcatctttga atgcaagaga acctgcaatg
     1921 tggaatgcca tttcagagga gtcgacttca tggtaggaac catcgtatac gttagcatat
     1981 acaccgacta cagggaatcc gccaaggata ccagctttca tagcttcttc gataccttcg
     2041 ccgactgccg gaatgtattc cttcggaata gcaccaccaa caactgtgga ttcgaacttg
     2101 tacagttctt ctccgttggc atccataggc tcgaatttaa ctttacagtg accatactgt
     2161 ccacgaccac cagactgttt tgcatactta ctgtctacat caacagcttt ggtgattgtc
     2221 tctttgtaag caacctgagg agcacctacg ttagcctcaa ccttaaattc acgaaggaga
     2281 cggtcaacga tgatatccag atgaagctca cccataccag cgataatagt ctgtccagtt
     2341 tcctgatctg tatgtgcacg gaatgtcggg tcttcctctg caagttttgc aagagcttca
     2401 ccaagtttac cctgtccagc tttagtctta ggctcgatag ccagctcgat aactggttct
     2461 gggaattcca tggactccag gattaccgga tgctgctcgt cacagattgt atcaccagtt
     2521 gttgtgaatt tgaatccgat agctgcagcg atatcaccgg aatatacttt atcaagttcc
     2581 atacgtttgt tagcatgcat ctgaagaata cgtccaacac gttctttctt gtccttagta
     2641 gcattaagta cataagaacc agaattcatt gtaccggagt atacacggaa atatgccagt
     2701 ttaccaacaa atgggtctgt cataatcttg aatgcaagtg ctgcaaacgg ttcttcatca
     2761 gaagaatgtc ttactaccgg gttgccgtcc aggtcagttc cctcgattgg aggaatatct
     2821 gttggagcag gcatatactc aaggatagca tcaagaagtt tctgaacacc tttgtttctg
     2881 taagcagaac cacagcaaac cggaacagct gtacactcgc atgtcgcttt tcttaatgtt
     2941 gctttcagtc tctcaacagt tggttcttca ccttcaagat attccatcat taagtcgtca
     3001 tcaagctcac agattttctc aatgagctct gtatgataaa gctcagcatc ttccttcatg
     3061 tcttcaggaa tgtcaacgat ggagatgtca tcaccttttt catcgttgta gatgtaagct
     3121 ttcatttcga ataagtcaat gattcctttg aaatcatctt cttttccgat tggcagctgt
     3181 aagcagattg cgtttttacc aagacgagtc ttgatctgct ctactgcacc gtagaagttt
     3241 gcaccaagga tatccatctt gttgatgaat gccatacgtg gtacattgta agtgtcggcc
     3301 tgacgccata cgttttcaga ctgcggttca acaccaccct tagcacagaa aacgccgaca
     3361 gcaccgtcaa gtacacgaag agaacgctca acttctactg tgaagtcaac gtgacctggg
     3421 gtatcaatga tgttgatacg atgttccaga gcacctggct tcggtttgca gttctcttcc
     3481 agagtccagt gacatgttgt agccgctgaa gtgattgtga tacctctctc ctgctcctgt
     3541 tccatccagt ccatggtagc agtaccttca tgagtatcac caatcttata gttgacacct
     3601 gtataataca ggatacgttc tgtaagagtt gttttacccg catcgatatg agccataata
     3661 ccaatatttc tggtacgctc taatggatat tctcttccct ctttagccaa tggattttcc
     3721 tcctatatta gaagcgatag tgagcaaatg ctttatttgc ctcagccatc ttgtgcatgt
     3781 cttcttttct cttaacggat gcacctgtgt tgttcattgc atctaaaagc tcatttgcaa
     3841 gtctttcttc cattgttttc tcgcctcttt tacgagagaa cattgttaac cagcgaagag
     3901 ccagtgcctg acgtctgtct gctcttacct cgatcggaac ctgataagta gcaccaccga
     3961 tacgtctagc ttttacttca agaagtggca tgatgttgtt catagcctct tcgaaaactt
     4021 caagtgctgg tttaccagct ttttcttcga ttctggcgaa tgcaccgtaa acaattttct
     4081 gtgctacacc tttcttaccg tctaacatga tgttattgat aagtttggta accactttat
     4141 tgttgtacat tggatctgcc agaacgtctc ttttctgagt atgtccttta cgtggcacgt
     4201 tacttccctc cttaatcatt gtgattaaat catcggtact cacgcagata actattgcat
     4261 gttcgcactg gtaaggtcct atatttaacc cgcaaccggc agggtatatg gaataccgta
     4321 caaattcata gttctagtct tccgtgaatt ttcgattgat taattcctgc tgtcctaaac
     4381 aataggatca gttcttaaaa ttcttatgaa acctttttag aattatttct ttttcggtct
     4441 cttagcgccg tatttagaac gggcctgttt tctgttagca acacctgcag tatcaagagt
     4501 acctctgatg atgtggtatc ttgtaccagg taagtcctta acccttccac cacggataag
     4561 aacaacgcta tgctcctgaa ggttgtgacc ttctcctggg atgtagcttg ttacttcgat
     4621 tccgttggaa agacgaactc tggcgatttt tctaagagct gagttaggct tcttaggagt
     4681 tgcagtctta actgctgtac aaacgccacg tttctgtggt gcagactgtt caactgcttt
     4741 ctttcttaaa gagttgaatg ttttctggag agccggagct gtagatttct ttacagatgt
     4801 ctggcgtcct tttcttacta actggttgaa tgttggcatt ccatttcacc tcccgattta
     4861 tttctccata gatttttcta tggggccttg cggttgctat tcgtgtaatt acgcacaaaa
     4921 aaattaaacg ttccttttgc acgcttgctc attatatccc gtaaaactca cactgtcaag
     4981 gcatttctcc tgaattttac agtatttttt catattttta tcataaccaa aaaatccttt
     5041 cctcatacac tacatcacca tattaaactt atctgacaat atgctccccc tccaaccact
     5101 ttactgccac ttccccaccg cgaatcccag cgccggataa tatttatttt gtgtgcattt
     5161 gcaggatgct tagagatact taatctgcag aaatctgcgt tatgagcaga ccgtctcacg
     5221 tgtttgagcg aagtttgcaa cgtagcgagt ttgagagggc tgctcatgct tttagcagat
     5281 ttctgcagat ttatagcagt cctagtaaat aatgcattta agacagttca gcagagtgcg
     5341 aaagacaagg aagatatctg caggcgtgct aacgcacggc aaagatatct gacgcaggat
     5401 ttcacactct gatgggctgg attaagatgc attatttact agaactgcta taatatctct
     5461 tagcgtccga aaccgcacac aaaataaata ttatccggcg cgtccccttc actaacaatt
     5521 gcatttcccc ctccctttta ttataataac aaccaaagca ggcaaatcaa cttgcagaca
     5581 cactataaca ttctataaac tcaggaaagg acactataat atatgaagaa acccagcatg
     5641 atcagcatcc ttctgtttct gacaggactc ctgtgcatca tagccctgat cttctttaac
     5701 aggaccacac tgcaccggat cctcaccact gaaaaagaag tcgacacaat ctccgaacag
     5761 atcactgcaa aacacgacga agtcaaacag gcagtagaca agtacactac cgccatagac
     5821 acagtccagt caaatatcaa ccgcatgaaa caaaaacagc agacagccac acagaagaaa
     5881 acaaaagaaa aagctgaaat cccggaagaa gcttcatcaa agccttctgt attcagccag
     5941 aacagcacca gtgcctcttc tgattccagt gccagcgttt tctcctccga tgccgaaaca
     6001 gacggacata taataggaat cgatccaggg catcagagtg agtccgtgga tatgagtgcc
     6061 ccggagccca acggtcccgg ttcttctgaa atgaaagcaa aatgtaccag cggaactcag
     6121 ggaacctaca gcggtgttcc cgaataccag ctcaatctgg aggtctctct tcaactaaag
     6181 gatgaactgg aacagagagg ctatcaggta gtcatgacac gaacagacaa tgaaacagcc
     6241 atcagcaaca tggaacgcgc acaatatgct gctgcacagg gcgcagagat ctatgtccgc
     6301 atccatgcaa acggcgatga ttcccatact gcatcaggag cactgaccat gtcgccctct
     6361 cagaataacc cttatatccc acaactgttt gaacagtctg atcgattatc ccggtgcatc
     6421 atcgattcct actgtgctgc aacaggtttt cagaaccttg gaatccagta cactgacacg
     6481 atgacaggaa ttaactggag cactgttcct gttacgatcc ttgaaatggg atttatgaca
     6541 agccagaatg acgatctgaa aatgaatgac gcagaattcc agaaaacaat ggtacaggga
     6601 atcgcaaatg gcattgacag ctactttgca tcatgatgat ataagtgtca aaaagcattt
     6661 tgcccctgac ttgatatctg cggattgcta cgcagcttcg cagttcccgc aatcctgatt
     6721 acaataataa taattacgaa aggaaaaccg caatgaataa aaagccaaat gaaaaaccta
     6781 tttttctcta tatagtgatc ggcatactga ctgttgcagt gattgcactg gcagctgttt
     6841 catttttatc actgaaccat ctcgtcacac tacagcaaaa agtacagaca ctctccaaaa
     6901 ccgtagagga gatttccact gatgcaaata cccttatcag tcaggcagat cagttagatg
     6961 aattaagaga acaggaaagt tctgtcaagg cagccaccgg agaaacatct cctgcacagt
     7021 cagaatccgc tgaaccctct tcagaatccg aacagcagaa cggaacactt tccccttcct
     7081 ccaacaacac atttaccgac aacactgatt ccagcatgga caatctcctg aaacaggtac
     7141 agtccctgct gccggctgat aacggaacct ggtccgtcta tgtctgcaac ctgccgaaag
     7201 attctgaagg catgatcaac gatactccca tgcaggcagc aagcctgatc aaactgtata
     7261 ttatgggagc tgtatacgaa aactacgata ccctctccca gtctcacaac ggagacgaaa
     7321 ttgacagtaa tatttcagcc atgatcactg tcagtgataa tgatgctgcc aacactctgg
     7381 taaactggct tggaaacggc gatgactctg caggcatggc aaaagtaaat ggcttctgcc
     7441 aggaacatgg tttcaccagc acccagatga accgcctgct tctcgcaagt aaagaaaacg
     7501 gcgacaacta tacatccgtc aaagactgtg gaaccttttt aaagcagata taccagactg
     7561 tcaatggaac ccttcccgct tctactcttc caaacgcaga tgcaatgtat tatcacttaa
     7621 aaatgcagca gcgtaaaaac aagatccctg cacaacttcc agaaggcgtc ggtactgcca
     7681 ataagaccgg cgaacttgac actgtagaaa atgatgcagc gatcatctat gatacagcca
     7741 aaggaattga tcttgtagta tgctttatgt cacaggatct tactgatacc ggtgcagccc
     7801 aaagcaccat tgctgcagat gcacgtgcta tctacggata ttataatgaa taaacacact
     7861 cttctgaatc tacagtctat ccagataatt gtcataaatc agacagatat tcgcaacctc
     7921 cgcaggggac ttacttgatt cggttaaaag aaggaggcac gtcccttaac gggttgtgcc
     7981 tccttctgca aattcaacag gaagaatcgt caatgtctca atctcttccc cttttatttt
     8041 ctttaataaa agttccacag cctttcgtgc catatccggc accggctgta caattgttta
     8101 taccattatg cctcttcagc ttccccctct tcataggact tctcccatat ctccgaaaat
     8161 actgccagcg gacagtctgt gagaagacac atgagaagga aacattcgta tggatttact
     8221 atgtatatct ctcccattcc acatcgaagc agaatatcct gaatctcatc cagaaaatgc
     8281 ttgtatctgt taaaaggatc atcatcctcc atttcctggg atacgataaa gaattccagt
     8341 gtgatcaggt caaagcgctc caccgggagt ttgtggctta aaatatttgt aatcctctgc
     8401 ctgctgaaac gcttctggct gaaatgcttt gccagaatgg aggcagacat tttcttcaga
     8461 ttgcccattt tgttgatggg gattccgctg cagattactt tttccacatc agaaggtgta
     8521 attctttctg ctgtccacac cttatcccca cctttttcct cttcatcatg ctgatacagt
     8581 ccggcaataa tctgcttcgc atgatataaa agctttgtaa actcctgaaa tgcctgactt
     8641 ttctcagata tgggatctac tctttttgat ttcagtcggg caagataatc gatcagtttc
     8701 tcctccgtat caaggcacag gttgcttccg tataaaacct gagtattttc cacttccaca
     8761 ggagcgagta tctcatattt tttcttcaat tcctctgctt tgtgataacc ctgctgcgta
     8821 ctgtaacaat accagtagat cacctcgtcc ggattgtgaa aatcaaaatc ctgttctttc
     8881 agtactcttg tcaggaaatc cgacacatcc tctgtggtca ttttcaaccc aaaccccaga
     8941 agaaaaacag tttctctttt tacagatgcc tgattcagcc agttattcac cagtgccgac
     9001 aacttggtag aggtaggatt catggatttg ggcgtatagg tttccttaaa ggaatctact
     9061 acaatctcct tatacacttc ctgtggaacc tccgagaagg gttcttccag ctctgccctt
     9121 tcataaatat atctcttcag atggtcgcca aaggacacca gttccatctc tttatataaa
     9181 tagtggaaaa taacatccgc atcttcgtct tcaaaactgt ccaggcttac cacctgccga
     9241 aatctctgtg ccgcccttct ggtaaattca tactcctgta ctttatcaaa cgcaacattc
     9301 tgttcaaaat ccagatcctg catacggttc ttcatgctgc cccacctctc attttttatc
     9361 tttcagattt ttcatccatt ctttcattct gcggactgta tccagcttct gtacttcaca
     9421 gagcacgatc gtcacattat ctctcccacc tttttggaga gcatttttaa gaagatcatt
     9481 tacgatctcc gggatttcca tatcctgtga cagaatcgcc tctatctcac tgtccgaaag
     9541 catatctgtt actccatctg aacacagaag atatctgtat ccctccttat gctcgatttc
     9601 tgtcacagaa ggttccagtc tctgcagctc ttcaggaagt ccaagatact gggttaacgg
     9661 ggctttccca actgccgtac caccaaaaac atggtctgtg gaaatctgct gaagcttacc
     9721 accatccata aaataaatcc tgctgtcccc aagattgcag gcaaacatag actctggtgt
     9781 aaagaggagc atcgccattg tgcttcccat gctccaaata tgatgctctg ttccatatct
     9841 gcatactgcc tgattcatct tctcacatac atcgtctata aaatcttccg gcatatccct
     9901 cagcattctt ttattcgcat tataaaattt tccaaattct tctgatgcaa gaaaggctgc
     9961 aatctcgcca cagctttctc ctcccatacc gtcaaatacc gccattgccg gtactctgtt
    10021 acccgaaata atctttgagc agataccctt tgttccttca ttgttcaccg gaagggattc
    10081 cccaaaacac cagaaattat cttcattatt tgcccggata tttcctgtat ggcaggtata
    10141 tgcacattca atctgataca ttgccattat ctgtctttgt tccttctccc ttcaatgctt
    10201 tcaatgaatt gttccagtat ttctcgccac gtttcacgaa ttcctcatta tctttttcgt
    10261 tatcgtgaca ctttccattg gaatcgatcc atcttatcag ttcgccgtcg ttataataat
    10321 aatattctga agtttcatca taccagatat atgcaaagaa taacttctct ccccagtaga
    10381 aatattcttc ataaactcca tctccggatt ctgccggata gaccagagct ttcat
//
LOCUS       DBMDXG010000024        10149 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1836_length_10149_cov_2.761744, whole genome shotgun sequence.
ACCESSION   DBMDXG010000024 DBMDXG010000000
VERSION     DBMDXG010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10149)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_10149_cov_2.761744"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            268..>5091
                     /locus_tag="ACZPD3_01565"
     CDS             268..>5091
                     /locus_tag="ACZPD3_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1414731.1"
                     /translation="MTNLLALHFRDTCRNWEKKQSYSDWQDVVSGAWTDQEEADAIVY
                     RFEWLQKYMDENPGDYHRWEIQHNPEYFQRFQDLQRERSWVDQKFTVERPVLSFITQD
                     EIDAVLRRGGITAGGRNRIYEYFMEHYDMKDAAEFMKNEYGTGGASPGIPGAYESDAS
                     HDAKGLKLAKGKIGSPEVEILLKWNKVAERVRQLIRTDDFLSPEELEKYEERQEAQRL
                     ADLEEAQQMLGEQLEQDTLTAEDITDLRLVDSEYMSGTRTKIHDFDCKVKGEANRLQY
                     TLEYHDDGEGFTIHTEKDDIWNRMSTQELERLDVKLGQEVLYYHYHNKTVNADTLDEL
                     REIREEIMEEESSYFTAISQRVWTDYDKKEKELSGEAEVSEEKESLEETNVTAEQIPA
                     TNFRITDDELGQGTAKEKFRANIMAIQLLKKCEDENRNATPEEQEILSRYVGWGGLAD
                     AFDETKAAWETEYLELKTVLTPEEYAAARASTLNAHYTQPIVIESMYQVLENLGFTKG
                     NILEPSMGVGNFFGMIPENLNQSKLYGVELDSISGRIAKLLYPDANIQIKGFEKTDYP
                     NDFFDVAIGNVPFGAYKVNDRQYDRYNFMIHDYFLAKTIDQLRPGGVAALITTKGTMD
                     KASPEVRKYLAERADLLGAIRLPNTAFKANAGTEVSADILFFQKRESLTKEMPEWINL
                     DSDVNGITVNQYFVQHPEMILGEMKEVSGPYGMETTCAPMEGADLELLLQEAVKHIKG
                     SMAPAVDVETELDEMPESIPADPNVRNYSYTVVDDQVYYRVNSLMNQVKMPAATAERV
                     KGMVAIRDTVRELIAMQMEEFVTDEEIQKQQEKLNQVYDTYTAKYGVIGSNANKRAFS
                     DDSSYCLLCSLEDLNEDGTLKRKADMFTKRTIKKAVAVTSVETATEALALSLNERAKV
                     DLPYMAQLTGKTEEKITEELVGVIFKNPLTDQWESGDEYLSGNVRDKLNTSRTFAENH
                     PEFTSNVRALEAVQPRDLEASEIEVRIGATWIEPSDYQDFMQETLHTPWYLLQKEIQV
                     KFSEVNGEWRITGKNADSPQNAFAYATYGTERANAYKILEDTLNLKDVRIYDKSVNEN
                     GDEIRVLNKKETMLASQKQDAMKAAFKDWIFKDQQRRERLVKVYNERFNSIRPREYDG
                     SHLTFPGMNPEIELRPHQKNAVAHQLYGENVLLAHVVGAGKTYEMVAAAMESKRLGLS
                     QKNLFVVPNHLTEQWGAEFLQLYPGANILVATKKDFEPANRKKFCARIAMGNYDAIII
                     GHSQFERIPISDERQEAMLRKQIDDLEMAIQSARYEQDGGRYTVKQIEKTRKTLQTRL
                     EKLNQKEKKDQVVTFEELGVDHLYVDEAHSYKNAFLYTKMRNVAGIAQNEAQKSADMF
                     NKCQYLDEITRGKGITFATGTPISNSMTELYVMQRYLQNSKLQNMGLGLFDSWASTFG
                     EVVTSIELAPEGTGYRAKSRFARFYNIPELMNMFKEIADIKTSDQLKLPVPEAEYETV
                     VLKPTEQQKEIVESLGERAEVVRNGGVDASVDNMLKITNDGRKLALDQRLVNELLPDN
                     PESKIAVCAEKSYEIWKDTAAQKSAQLIFCDLSTPKGDGSFNVYDDLKQKLIEKGV"
     assembly_gap    5092..5101
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5102..6311
                     /locus_tag="ACZPD3_01570"
     CDS             <5102..6311
                     /locus_tag="ACZPD3_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1414732.1"
                     /translation="VPEKEIAFIHDANTEAKKTELFGKVKSGQVRFLIGSTAKMGAGT
                     NVQDRLIALHHLDIGWKPSDLEQREGRIIRQGNHNKKVHIFRYVTESTFDSYMWQLIE
                     NKQKFISQIMTSKAPVRSCEDVDEAALSYAEVKALATGNPAVKEKMALDVDVAKLKLL
                     KANHMNNQYRLEDDIARNFPQQIAKLTEIIDSYKADIAHFSEHKITDPEQFSMEISGK
                     VFTEKKEAGEALLAVCKDIKSVDAAMDIGSYQGFNMRIQFDSWSKEFILSVKHESVAK
                     VRLGADALGNIIRINNLLESYPEKLAEAEQRLETVQEQMTNAKEEVGKPFPKEEELNQ
                     KLERLSELNALLNMDEREDTETEQSESKEKEERPARGSIHEKLQIYKEKSQRESETGK
                     ETRKRDFGLE"
     gene            6392..9151
                     /locus_tag="ACZPD3_01575"
     CDS             6392..9151
                     /locus_tag="ACZPD3_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HIR1414733.1"
                     /translation="MARNMITGKETQKMQEFTQEQMDRAEHMGIALPVDVKEQIFEYA
                     NLIGEEEKVRTLVKNLTDAINQADEDKVEELLDDARMDIQDLPDPTIGKLELRDYGYT
                     AEDMVPLRKEAALDYHRMGSKIYCLGSDGGKGEYASKEMIQAHEGLFGMELQMWERIR
                     DQDLDYADEDFGAFQEPMSVIDQEEALKLYDAGADIYLITNFSSPIYVTERKEIERGP
                     EHYQMSMAERERFRNLEWEMQKYPQIQSLKEANLLLGTRRTFGIYQIKDDSPGENYAF
                     MNMSFIESHGMQIKKEDYELVYVGELLGNTSLDDIFERFNIDRPKDFRGHSLSVSDIV
                     VLNDGEKVTAHFVDSISFEQLDSFLNLEEQVLSELAYEVGERYFAIQRTEEGYDYSFY
                     DEDFRLMDGGVYENDEISIEEAAEELLEDEGWTGERIRGDYDQLMEKVKEMDEVVMAE
                     IQKSQGEYKPLAKVEELEEANYNMIDNVLNNMPLKKEPYLEYFATECDEFHDMGAYEK
                     STDVNQIAAVYEKYKENPETAYLGCSMGIIYRDPEDSYYDDAEFAIVKGNTVLGNLMD
                     DVRFYGELALVREGIEKIHEALPDYKYVPMRDVREAMYPEKMTTEQLAEALDEIAEAF
                     DPYDYRDHVEPGQDTLQEVMLDLQSGNVGSYISFLKDVIEEDCEQSVWAGVLLERLKS
                     YEPDISKETEPMVYVNYCEKRELMEPRCQKLSDLDSRTAQKDKEWYADRNPRTDEPMV
                     TAQMFFTIYYAEKGDKMLQYFKGKIDIGTGNGGILSQLKLQNELKLTDESWIGYLKNK
                     GNEEFQKCMEDLTDMQNHVLPYLQSFCSLEEKSVQEKQERQVAEKQDSRVEASRAANV
                     TKGDNDKAVKKVVSSMQTEKNKATTKAKKPSIHERLEINKRKIQEKQGKDDPERGADR
                     DVRTV"
     gene            9179..9385
                     /locus_tag="ACZPD3_01580"
     CDS             9179..9385
                     /locus_tag="ACZPD3_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024861.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR1414734.1"
                     /translation="MDIAKVLTVTNEDVLPAYLQRVSDFEDCLLATCTKANQCDAIVT
                     RNKKDFLSFWITLLSPEELLNIYS"
     gene            9732..9875
                     /locus_tag="ACZPD3_01585"
     CDS             9732..9875
                     /locus_tag="ACZPD3_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1414735.1"
                     /translation="MNEAAVCSGVPPSTLKNILYGQSKNAGVVTIKKICDGLDVTIQE
                     FFC"
BASE COUNT         3443 a         1636 c         2619 g         2441 t
ORIGIN      
        1 gaaggtctgg ataatgaaga aatgcggagt tttctgaaag atgaatatgg aactggtggc
       61 aaaggattta ccatagatgg tcagaaaatc agtatctggt atgacaatga tggtatccgt
      121 atccggcgag gggactctgc cagaagaaac tttgaccgca tggttacgtg ggaagaagca
      181 gcgaacagaa ttcgggatat gtatgaggat ggaaattacg tggataattt gatttccaat
      241 aatgccattg aacaggagca ggaagaaatg acgaatcttc ttgcacttca ttttcgggat
      301 acttgcagaa actgggaaaa gaaacaatca tattcagact ggcaggatgt agtgagtgga
      361 gcatggacag atcaagagga agcagatgcg attgtctatc gttttgaatg gctgcagaaa
      421 tatatggatg agaatccggg agattatcac cgctgggaga ttcagcataa tccggaatat
      481 ttccaacgct ttcaggatct tcagagggag cgttcctggg tagaccagaa atttacggtc
      541 gaacgtccgg tactttcctt tattacacaa gatgaaatag atgctgtcct tagaagaggc
      601 ggcattacag ccggaggacg caatcggatc tatgagtatt tcatggagca ttatgatatg
      661 aaagatgcag cagagttcat gaaaaatgag tatggaacag gtggtgcatc acctgggata
      721 ccgggagcgt atgaatctga tgcctcacat gatgcaaaag gattaaaact tgcgaaagga
      781 aagattggca gtccagaagt agaaattcta ttaaaatgga ataaggttgc agaacgtgtc
      841 cgtcagctga tccgcaccga tgatttctta tcaccggaag aattggaaaa gtatgaagag
      901 cggcaggaag cacagagact ggcagatctg gaagaagcac agcagatgtt aggagaacag
      961 ctggagcagg atactttgac agcggaagat ataactgatt tgcgattagt tgattcagaa
     1021 tatatgtctg gtaccagaac aaaaattcat gattttgatt gtaaagttaa gggagaagcg
     1081 aatcgacttc aatacacatt agaatatcat gatgatggag aaggtttcac gattcatacg
     1141 gaaaaagatg atatttggaa tcgaatgtca acacaagaat tggaacgtct ggatgtaaaa
     1201 ctcggacaag aagtacttta ttatcattat cacaacaaaa ctgtaaatgc ggatacctta
     1261 gatgaattac gggaaatcag ggaagaaatt atggaggaag agtcctcgta ttttactgct
     1321 atttctcaac gtgtgtggac agattatgat aaaaaagaaa aagaattatc gggagaagct
     1381 gaggtatcgg aagaaaaaga atctttggaa gagactaacg tgacagcaga acagattcca
     1441 gcgacgaact tccgcatcac agatgatgag cttggacagg gtacagcaaa agaaaagttc
     1501 cgtgccaata tcatggcaat ccagttgtta aagaaatgtg aggatgagaa tcgcaatgcg
     1561 acaccagagg aacaggaaat cttatcccgc tatgttggct ggggaggtct tgctgatgca
     1621 tttgatgaga caaaagcagc atgggagaca gaatatctgg aattaaagac ggttcttaca
     1681 ccagaggaat atgctgcagc cagagcttcc accttaaatg ctcattatac acagccgatt
     1741 gtgattgaaa gtatgtatca ggtgttggag aatctgggat ttacaaaagg aaatattctg
     1801 gagccgtcta tgggagtcgg gaacttcttt ggaatgattc cggagaatct aaaccagtcc
     1861 aaactctatg gtgtggaact ggatagtatc agtggtagaa ttgcaaagct gctttatccg
     1921 gatgccaata ttcagatcaa aggttttgag aagacagatt atcccaatga tttctttgat
     1981 gtggcgattg gaaatgtgcc attcggtgca tataaggtca atgaccgtca gtatgatcgc
     2041 tacaatttca tgattcatga ttattttctt gccaagacta tagatcagtt acgtccaggt
     2101 ggtgtggctg cgttgatcac aacaaaggga accatggata aggcatcacc ggaagttcgg
     2161 aaatatctgg cggagcgggc agacctgctg ggagcaattc gactgccgaa cacagctttt
     2221 aaggcaaatg caggaacaga agtcagtgct gatatcctgt ttttccagaa gagggaaagc
     2281 cttacgaaag aaatgccgga atggataaat ctggatagtg atgtcaatgg tattacagtc
     2341 aatcagtatt ttgttcagca tccggaaatg attctgggtg agatgaaaga agtgtccggt
     2401 ccgtatggta tggaaacaac ctgtgctcca atggaaggag cagatctgga attattgtta
     2461 caagaagctg tgaaacacat caagggaagc atggcaccgg cagtagatgt ggagacagaa
     2521 ctggatgaga tgccagagag tattccagca gatccaaatg tgcgaaatta cagttatacg
     2581 gtggtagatg atcaggtata ttaccgtgtc aactctttga tgaatcaggt gaaaatgcct
     2641 gcagccactg cagaacgtgt aaaaggcatg gttgcaatcc gtgataccgt tcgtgagctg
     2701 atcgccatgc agatggaaga atttgtcaca gacgaagaaa tccaaaaaca gcaggaaaaa
     2761 ctgaatcagg tgtatgatac ctacacagca aagtatggag ttattggaag taatgctaat
     2821 aaacgggcat tttctgatga ctcttcttat tgtctgctgt gttccctgga agacttgaac
     2881 gaagatggaa cattgaagcg gaaagcggac atgttcacca aacgaaccat caaaaaggcg
     2941 gttgctgtga caagtgtgga gacagccaca gaagcacttg ctctgtcttt aaatgaaagg
     3001 gctaaagtag atctcccata tatggcacag ctgaccggaa agaccgaaga gaaaattaca
     3061 gaagagctgg ttggagttat cttcaaaaat ccactgacag accagtggga aagcggagat
     3121 gaatatctct ccggaaatgt tagggataaa ttaaatactt cgagaacttt tgcagagaat
     3181 catccggaat tcacatcgaa tgtgcgtgca ctagaggcag ttcagccgag agatctggaa
     3241 gcatcggaga ttgaagtgcg tattggagct acctggattg aaccttcgga ttatcaggat
     3301 tttatgcaag aaacattgca tacaccatgg tatctgcttc agaaagaaat acaggtgaaa
     3361 ttctctgagg taaatggtga gtggaggatt acaggaaaga atgccgacag tccccaaaat
     3421 gcgtttgcct atgcaacgta tggaactgag agagcaaatg cctataagat tctggaagat
     3481 accctgaacc tgaaagatgt acgcatctat gataagagtg tcaatgaaaa tggtgatgag
     3541 atccgtgtcc ttaataagaa agaaaccatg ctggcatcac agaagcagga tgccatgaaa
     3601 gcagcattta aggactggat ttttaaagac cagcagagac gagagaggct ggtaaaagtg
     3661 tacaatgaac gatttaacag catccgtccc cgtgaatacg atggcagtca tttaactttc
     3721 ccaggaatga atccggaaat tgaacttcgc ccacatcaga aaaacgctgt cgcacatcag
     3781 ctttatggag agaatgtgct tcttgctcat gtagtaggag ctggaaagac ctacgaaatg
     3841 gtagcggcgg caatggagag caaacgcctg ggattatctc agaaaaacct ctttgtcgtt
     3901 ccaaaccatt tgacggagca gtggggtgca gaattcctgc agttatatcc gggtgccaat
     3961 atcctggtgg caacgaagaa agattttgaa ccggcaaatc gaaagaagtt ctgtgccaga
     4021 atcgctatgg gaaattatga tgctattatc attggacatt cccagtttga gcgtattcca
     4081 atctctgatg aacgtcagga agccatgtta cggaagcaga ttgatgattt ggaaatggca
     4141 attcagagtg caaggtatga acaggatggc ggacgttata ccgtcaaaca gattgaaaag
     4201 accagaaaga cactgcagac aaggctggaa aaactgaatc agaaagagaa aaaagatcag
     4261 gtagttacgt ttgaagaact gggcgtggat cacttgtatg tagatgaagc acacagctat
     4321 aaaaatgcgt ttctttatac aaaaatgaga aatgtagccg ggattgcaca gaacgaagca
     4381 cagaagtcgg cagatatgtt caataaatgt cagtatctgg atgagattac cagaggaaaa
     4441 ggcattacct ttgctacggg cacaccgatc agcaacagca tgacggaatt atatgttatg
     4501 cagcggtatc tgcagaacag caaactgcag aatatgggac ttggattgtt tgattcttgg
     4561 gcttccactt ttggagaagt tgtcacgagc atcgaacttg caccggaagg gacagggtat
     4621 cgtgcaaaat ccaggtttgc tcgattctat aatatcccgg aactgatgaa tatgtttaag
     4681 gagattgcag atatcaagac ttccgatcaa ttaaagcttc cagtgccgga agcggaatat
     4741 gaaacagtgg tgttgaaacc aacggaacag cagaaagaaa tcgtagaaag tctgggagaa
     4801 cgtgcagaag ttgtcagaaa cggaggggta gatgcctcgg tcgataatat gctgaaaatt
     4861 accaatgatg gaagaaagtt agcattggat cagcgtttgg tgaatgaatt attgccagat
     4921 aatccggaaa gcaaaatagc agtctgtgca gagaaaagct atgaaatctg gaaagataca
     4981 gcagcacaga aatcagcaca gttgattttc tgtgatttga gcacaccgaa aggcgatggc
     5041 agtttcaatg tttatgatga tttgaagcag aaactgatag agaaaggtgt annnnnnnnn
     5101 ntgtaccgga aaaagagatt gcgtttattc atgatgcaaa tacagaagca aaaaagacgg
     5161 agctgtttgg gaaagtgaaa tccggacagg ttcgtttttt gattggctca acagcaaaga
     5221 tgggtgcagg aaccaatgtg caggatcgtt tgattgccct gcatcatctg gatattggtt
     5281 ggaaaccatc tgacttggaa cagagggaag gacgtattat ccgtcaggga aaccataata
     5341 aaaaggttca tatcttccgg tatgtaactg aatccacatt tgacagctac atgtggcagt
     5401 tgatcgaaaa caagcagaag tttatttccc agatcatgac cagcaaagca ccggttagaa
     5461 gctgcgaaga tgtggacgaa gcggcactgt cctatgcaga ggtcaaagca ctggcaaccg
     5521 gaaatccagc agtaaaagag aagatggcat tggatgtgga tgtggcgaaa ttaaagcttc
     5581 taaaagcgaa ccacatgaat aaccaatacc gactggaaga tgatattgca aggaattttc
     5641 cgcaacagat tgcaaaactg acggagatta ttgacagtta taaggcagat atcgctcatt
     5701 tttctgagca taaaattaca gatccagagc agttttctat ggaaatcagt ggcaaagtgt
     5761 tcacagaaaa gaaagaggca ggagaggctc ttctggcggt ctgcaaagac attaagtctg
     5821 tagatgcagc tatggacatt ggaagttatc agggatttaa catgagaatc caattcgaca
     5881 gctggagcaa agagtttatc ctgtctgtaa agcatgaatc ggtagctaaa gttcggttgg
     5941 gagccgatgc cctgggaaat atcattcgta tcaataatct tttagaaagt tatccggaaa
     6001 aactggcaga agcagaacaa cggctggaaa cggtacagga gcagatgaca aatgccaaag
     6061 aggaagtcgg gaagccattt ccaaaagaag aggagttgaa ccagaaactg gagcgactgt
     6121 cagagttaaa tgcacttctc aatatggatg agcgggaaga tacagaaaca gagcagtcgg
     6181 aatcaaaaga gaaagaagaa agaccggcac gaggttctat tcatgagaaa ctgcagattt
     6241 ataaagaaaa gagtcagcgg gaaagtgaaa ctggcaagga aaccagaaaa cgggacttcg
     6301 gtctggaata aataaacagg aaagatggca taatttggta gaagaaggtt ctgccggtta
     6361 tgcctttttt tagaacacag gaggaattta tatggcgaga aatatgataa caggaaaaga
     6421 aacgcagaaa atgcaggaat ttacgcagga acagatggac cgtgcagaac acatgggaat
     6481 agcgcttcca gtagatgtaa aagaacagat ttttgaatat gcaaatctga ttggcgagga
     6541 agagaaagta aggacgttgg taaaaaatct gacagatgca atcaatcagg cagatgaaga
     6601 taaagtagag gaacttctgg atgatgcaag aatggatatt caggacctac cagatccaac
     6661 cataggtaag ctggaattac gtgattatgg atatacagca gaagacatgg tgccgctcag
     6721 aaaagaagca gctttggatt atcacagaat gggttctaag atttattgcc tgggcagcga
     6781 tggcggtaag ggagagtatg caagtaaaga aatgatacag gcacacgaag ggctgtttgg
     6841 catggaatta cagatgtggg aacggataag ggatcaggat ctggattatg ccgatgaaga
     6901 ttttggagca tttcaggagc ctatgagtgt cattgaccag gaagaagcac tgaaattata
     6961 cgatgccgga gcagatatct atctgatcac taatttttca tcaccaatct atgtcacgga
     7021 acgcaaggaa atcgaacgag gaccggagca ttatcagatg tccatggcag aacgggagcg
     7081 tttccgtaac ctggaatggg aaatgcagaa atatccgcag attcagtctt tgaaagaggc
     7141 aaacctgttg ttaggaacaa ggcgaacctt tggtatttat cagatcaaag atgattcacc
     7201 gggcgaaaat tatgcattta tgaatatgag ttttattgaa agtcatggta tgcagataaa
     7261 aaaagaagat tatgagctgg tctatgttgg agaattactg ggaaatacgt cactggatga
     7321 tatttttgaa aggttcaaca ttgatagacc gaaagacttc cgtgggcatt ccctgtctgt
     7381 cagtgatatc gttgttttga atgatgggga aaaagtaaca gctcattttg tagacagtat
     7441 cagttttgaa cagctggatt ctttcctgaa tctggaagaa caggttctta gtgaactggc
     7501 atatgaggta ggagaacggt attttgctat tcagaggaca gaagaaggat acgattattc
     7561 cttttacgat gaagatttcc gtctgatgga tggtggcgtc tatgaaaatg atgaaatttc
     7621 tattgaagaa gcggcagagg aacttttgga agacgaaggc tggactggag aacgcatccg
     7681 tggggattat gatcagttga tggaaaaagt gaaagaaatg gatgaggttg taatggcaga
     7741 gattcagaaa agccagggcg aatataagcc actggctaag gtagaagaac tggaagaggc
     7801 gaattataac atgattgaca atgtacttaa caatatgcca ctgaagaaag aaccttatct
     7861 ggaatacttt gccacagaat gtgatgagtt ccatgatatg ggagcttatg aaaaaagtac
     7921 cgatgttaat cagatagctg cagtctatga aaaatataag gagaatccag aaactgcgta
     7981 tctgggatgc tcgatgggaa ttatctatcg tgatccggaa gacagttact acgatgatgc
     8041 tgaatttgca atcgtaaaag gaaatacagt acttggaaat ctgatggatg atgttcggtt
     8101 ttatggagag cttgcactgg tacgggaagg gattgagaaa attcatgaag cattgccgga
     8161 ctacaagtat gtaccgatga gggatgttcg agaagcaatg tatccagaga agatgaccac
     8221 agaacagctg gcagaggcac tggatgagat tgcagaagca tttgatccgt atgattaccg
     8281 ggatcatgta gaaccagggc aggatacctt acaggaagtc atgctggatc tgcaaagtgg
     8341 aaatgttggt tcttatattt cctttctgaa ggatgtgatt gaagaagact gtgagcagtc
     8401 cgtgtgggca ggtgttttgc tggaacggct gaaatcctac gaaccagata tatccaaaga
     8461 aacagagccg atggtgtatg tgaattactg tgaaaagcgg gagctgatgg aaccacgctg
     8521 tcagaagctc tcagatctgg attcccgtac tgcacagaag gataaggaat ggtacgcaga
     8581 ccgtaatccg aggacggatg agccgatggt aacagcccag atgtttttta ctatatatta
     8641 tgcggaaaaa ggcgataaaa tgctgcaata tttcaaaggg aaaatagata ttggaacagg
     8701 aaatggcggt attttaagtc agctgaaact ccaaaatgaa ctgaaactta cggatgaaag
     8761 ttggattggt tatctgaaaa acaagggaaa tgaagagttt cagaagtgta tggaagatct
     8821 gaccgatatg cagaaccatg tgctgccata tttgcagagc ttttgcagtc tggaagaaaa
     8881 aagtgtgcag gaaaagcagg aacggcaggt tgcagaaaag caggacagca gagtagaagc
     8941 gtcaagagca gcgaatgtga caaaagggga taatgacaaa gctgtgaaaa aggtagtttc
     9001 gagtatgcaa acagaaaaga ataaagcaac tacgaaggca aagaagccat ctattcatga
     9061 acgactggaa atcaataaga gaaaaatcca agaaaagcag ggaaaagatg acccggagag
     9121 aggagctgat cgggatgtaa gaacggtata aatttataga aaggcacttg gctccgtttt
     9181 ggacatagca aaagtgctga cagtgacgaa cgaagatgtg ctgcctgctt atttgcagag
     9241 agtatctgat tttgaagact gcctgcttgc aacttgtacg aaggcgaatc agtgtgatgc
     9301 gattgtgaca aggaataaaa aggattttct ttccttttgg attacactgc tttctccgga
     9361 agaactgctg aatatttatt cgtaaaatat gtttttacaa tcggtagcag aaggaaagtt
     9421 gaatgataac tccgatccag catactggaa aatataaaaa ttttataaca aagtggaaaa
     9481 ccctgcgtag gatcagaaat gttctcacag ggtttcatgt atctaataaa atatagaaat
     9541 ttagctgatt tctgctagaa taagaaaaac tgttaaaaaa atatcgtaca tagatcaaaa
     9601 agactatata tgggcgacct tttaaagccg atttatataa aatagaagaa aaaaaggtgg
     9661 tcgataccgt ggatataaaa acagcggtca tatatcgttt aaacgatctg attaatcaga
     9721 aagatatcac agtgaatgag gcggcggtat gttctggcgt tcctccttcg acattaaaga
     9781 atattttgta tggtcagagt aagaatgctg gcgtagttac aataaaaaag atatgcgatg
     9841 gattggatgt cacaatacag gaattttttt gctgatccca tctttgcaga tttggaaccc
     9901 gaactatagg aagagtaaat gctcatagga ttcagattct gcaggatgga ttttttatga
     9961 aaaagagtta ggaaaaacta acctatgagg acatagatgt ataaaatagc aatttgcgat
    10021 gatgataaaa attataggga gaaaatagag aaggttatag aaacggaagg gattcttcct
    10081 aagagcgaga tacggtttta tgaatacgag tcaggaaagg aactactgga aaatgctgat
    10141 attttgcat
//
LOCUS       DBMDXG010000025        10112 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1842_length_10112_cov_1.815703, whole genome shotgun sequence.
ACCESSION   DBMDXG010000025 DBMDXG010000000
VERSION     DBMDXG010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10112
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_10112_cov_1.815703"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(255..344)
                     /locus_tag="ACZPD3_01590"
     tRNA            complement(255..344)
                     /locus_tag="ACZPD3_01590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Ser,seq:gga)
     gene            complement(506..591)
                     /locus_tag="ACZPD3_01595"
     tRNA            complement(506..591)
                     /locus_tag="ACZPD3_01595"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(554..556),aa:Ser,seq:tga)
     gene            complement(766..1458)
                     /gene="ispD"
                     /locus_tag="ACZPD3_01600"
     CDS             complement(766..1458)
                     /gene="ispD"
                     /locus_tag="ACZPD3_01600"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR1414736.1"
                     /translation="MSERNTAIVLAAGQGKRMHSKVQKQFLEIQGYPVLYYSLRCFQE
                     SPLIQDIILVTGEESISYCKEEIVQKYGFTKVSAVIPGGKERYDSVYAGLCECKDCEY
                     VLIHDGARPFVTEEILKRGLQKVKETGACVIGMPSKDTVKLSDEEGYVKETPNRKCVW
                     TIQTPQIFSYSLIREAHDSIRQKDMSKITDDAMVVEQETGAKVALAEGSYQNIKITTP
                     EDLDIAEAFLKH"
     gene            complement(1460..2479)
                     /gene="tsaD"
                     /locus_tag="ACZPD3_01605"
     CDS             complement(1460..2479)
                     /gene="tsaD"
                     /locus_tag="ACZPD3_01605"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR1414737.1"
                     /translation="MKDTLILAIESSCDETAASVVKNGRTILSNVISSQIALHTLYGG
                     VVPEIASRKHIEKINQVIEQALADADVTLDDLDAIGVTYGPGLVGALLVGVAEAKAIA
                     YAKKLPLVGVHHIEGHVSANYIEHPDLEPPFLCLIVSGGHTHLVIVKDYGEFEILGRT
                     RDDAAGEAFDKVARAIGLGYPGGPKVDKLSKEGNPNAIEFPKAKIGDCPYDFSFSGVK
                     SAVLNYINHAQMTGEEINRADLAASFQKAVVDVLVEHTMLAAKDYGMTKIAIAGGVAS
                     NGTLRAAMEEACKKNNYSFYRPSPIFCTDNAAMIGVAAYYEYIKGTRHGWDLNAVPNL
                     KLGER"
     gene            complement(2553..3461)
                     /locus_tag="ACZPD3_01610"
     CDS             complement(2553..3461)
                     /locus_tag="ACZPD3_01610"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR1414738.1"
                     /translation="MLDVCLVGTGGMMPLPRRWLTALMTRYNGSSLLIDCGEGTQVAI
                     KEKGWSFKPIDVICFTHYHGDHISGLPGLLLTMGNADRTEPLTLVGPKGLERVVNALR
                     VIAPELPFEIKFIEITQPEQVIELNGYRITAFKVNHNVLCYGYTLEILRQGKFSAERA
                     KEQDIPLKYWNPLQKGQTIEADGITYTPEMVLGPARKGIRLTYTTDTRPTESILRNAK
                     ESDLFICEGMYGEDDKADKARGYKHMTFREAAVLARDARVKEMWLTHYSPSLVRPDEF
                     MDKVREIFPNAYPGKDGKSLELNFEE"
     gene            complement(3547..3984)
                     /gene="rimI"
                     /locus_tag="ACZPD3_01615"
     CDS             complement(3547..3984)
                     /gene="rimI"
                     /locus_tag="ACZPD3_01615"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR1414739.1"
                     /translation="MILREMLVDDLDQVMEIEQDLFHVPWTKEGFFTFLTRDDAMFLV
                     VEEKEKILGYCGLLMVLDEGDITNVAVRRDRQKEGIGAFLMQSLIRLAAEREVTTIHL
                     EVRVGNETAIRLYERMGFTGDGIRKAYYSDPVEDALLMTRHPE"
     gene            complement(3981..4709)
                     /gene="tsaB"
                     /locus_tag="ACZPD3_01620"
     CDS             complement(3981..4709)
                     /gene="tsaB"
                     /locus_tag="ACZPD3_01620"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR1414740.1"
                     /translation="MKILALDSSGIVASVAVVEDDTLLAEYTVNYKKTHSQTLLPMLD
                     EIVKMTELELESVDAIAVAAGPGSFTGLRIGSATAKGLGLALKKPLVAVPTVDALAYN
                     LYDAQGLICPIMDARRKQVYTGIYRFEEHQLMTLKEQWAAPIEELLEELNQRGEMVTF
                     LGDGVPVFRELIAEKLQVPYSFAPAHVNKQRAAAVAALGSIYYKEGRTETAMEHIPEY
                     LRVSQAERERAEREKEQKPAGTKI"
     gene            complement(4719..5153)
                     /gene="tsaE"
                     /locus_tag="ACZPD3_01625"
     CDS             complement(4719..5153)
                     /gene="tsaE"
                     /locus_tag="ACZPD3_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR1414741.1"
                     /translation="MIIETKTPQETFEVGKKIGENAKPGQIYTLTGDLGVGKTVFTQG
                     VAAGLGITEPICSPTFTIIQEYESGRLPLYHFDVYRIGDIEEMEEIGYDDYFFGQGIC
                     LIEWADLIEEILPEKLIKVTIEKDLEKGFDYRRITVIGPENS"
     gene            5247..5765
                     /locus_tag="ACZPD3_01630"
     CDS             5247..5765
                     /locus_tag="ACZPD3_01630"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1414742.1"
                     /translation="MANPIVTITMDNGDVMKAELYPEIAPNTVNNFISLVKKGFYDGL
                     IFHRVINGFMIQGGCPDGTGMGGPGYSIKGEFTQNRFKNDLKHTAGVLSMARAMHPNS
                     AGSQFFIMHKDAPHLDGAYAAFGKITEGMDVVNRIAEEDTDYSDRPLDEQKIKSMTVE
                     TFGVDYPEPEKC"
     gene            complement(5820..6476)
                     /locus_tag="ACZPD3_01635"
     CDS             complement(5820..6476)
                     /locus_tag="ACZPD3_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HIR1414743.1"
                     /translation="MLEKYKTVYEGGEGEIVEKKSRFIATVRPVQTEEEALAFIEEMK
                     KKYWDARHNCYVYSVGKNREYTRCSDDGEPSGTAGRPMLDVILGEDIYNVAAVVTRYF
                     GGILLGTGGLVRAYSRSLQEGLAASTVIEKTYGISMEVVTDYTGIGKIQYIAGEQKLP
                     ILDSEYTDRVVLHLLVPVDQIAFVEKAITEGTNGRAKMKKEKDLYYSVIDGEVKVFTD
                     "
     gene            6678..9269
                     /locus_tag="ACZPD3_01640"
     CDS             6678..9269
                     /locus_tag="ACZPD3_01640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR1414744.1"
                     /translation="MNYGKKSTAKKRTALISRSSMMGKRARVSFIRVLFVSLIALCIA
                     VTCLGVGSFRGVIDTAPDVDDIDIMPLGYATFLYDDAGNQIRKLAAPDSNRLPVTLDQ
                     IPVDLQHAVVAIEDERFYEHNGIDVKGILRAGMKALTTGDFSEGASTITQQLLKNNVF
                     TNWTSESTQLERFTRKIQEQYLAVQVEKKTDKDTILENYLNTINLGAGSYGVQAAARQ
                     YFDKDVWDLNLSECATLAGITQNPTKFNPIINPDSNRKRRKEVLQHMLDQNYITQDQY
                     DETLADDVYSRIQAAQEKNSSTENTVYTYFEDELTDQIINDLMNIKGYTKKQATNLLY
                     SGGLKVYTTQDSKIQNILDEEYADPSNYPDTVQYELDYALTVTDPDGNQVNYSKEMLQ
                     LYFQNEDPDFDLLFDSPEDGQTYVDKYKASILANGSKVLAERVNFAPQPQSSMSVIDQ
                     HTGYVKALIGGRGEKTASLTLNRATDTTRQPGSTFKIVSTYAPALNEKGMTLATTFED
                     EPYEYPDGSPVNNATRSYNGTTTIRTAIQNSINVVAVKCLEKVTPELGLKYLDNFGFT
                     TLAHGTEADKDANGNVWSDANLATALGGITRGVTNVELCASYAAIANNGNYIKPIYYT
                     KILDHNGNVLIENTAAERSVIKESTAFLLTSAMEDVVKQGTGTACQLDNMPVAGKTGT
                     TEAYNDLWFVGYTPYYTCAVWSGYDNNEKLPDYARNFHKALWKKVMTRIHEGLPSKEF
                     EKPASVEKLSVCEETGLLPRAGCPVITEYFDIGTMPTEYCDQHFYDSDDYDYNYDTDS
                     SDQTDNTTDTDNSENSDNGDTDNSGDSNNTDDNGNSGDDGTDNTGGSDDNGDGNEDDS
                     SYQVDYY"
     gene            complement(9389..>10112)
                     /locus_tag="ACZPD3_01645"
     CDS             complement(9389..>10112)
                     /locus_tag="ACZPD3_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1414745.1"
                     /translation="PVNERGEVPCAVNLFWGFKEVTKELTELTGLPSKAGNDANVAAL
                     GEAWKGAAAGAKNVILVTLGTGVGGGIIVDGKIVGGAHGAGGEIGHAAVDHEEKEACN
                     CGNCGCLEQYASATGIVRVAQRTLAATDEQTVLRKFTKLSAKNVLDAFKEGDKVACDV
                     MAQVGEMLGGTLAMFACVTDPEAIVIGGGVSKAGQPLIDCIQKYYEKYAFTACKKTPI
                     ILATLGNDAGIYGSARMVIKEV"
BASE COUNT         2713 a         2502 c         1902 g         2995 t
ORIGIN      
        1 tttttggttt ttcaaaccgt tcttaaaaat gtaaagaatt ttcgagaatc gtatgtgttt
       61 cactgtttag ttatcaaggt tgtctgtctt gcgacagctt tgctatttta acatgttgtc
      121 ttctgtttgt caagaacttt ttaataagtt tttttcaaac ttcttcaaca tttgttcttt
      181 tcaacagctt ttcgagttta tcataactct tttgctttgt caagaacttt ttactttctt
      241 ttttcaagaa agaacggaga aggagggatt tgaaccctcg cgccgctatt aacgacctac
      301 tccctttcca ggggagcccc ttcggccagc ttgggtactt ctccaaattg ccctagattc
      361 gtcgtttcgc agaaacgact ttcttcacac tctcgtgtct ttatttagtt ctcttcgttt
      421 tcatcgaaga accttgtgca ttatatcgca cttttcattt gtttgtcaag tacttttttc
      481 aaaacttttt aagtgacttg gcaagcggag agagtgggat tcgaacccac gcgcccttgc
      541 ggacaaacgg ttttcaagac cgcctcgtta tgaccacttc gatatctctc catatgttgt
      601 tgactgcgtt cgttgtgttc tcaccgccga cgcaagtgat attctatcat caatacccgc
      661 atctgtcaac aactttttta catttttttc aatttttatt tttatcttcc cagatttccc
      721 ataaagcccg ctaaaatacg gcatttttca atcaccaaac attctttaat gtttcaaaaa
      781 tgcttccgcg atatcaagat cctccggtgt tgtgatctta atattctgat aagatccctc
      841 tgccagagca acctttgccc ctgtctcctg ctctactacc atggcatcat ctgtaatttt
      901 gctcatatct ttctgacgaa tgctgtcatg agcctcacgt atcagtgaat acgaaaaaat
      961 ctgcggggtc tgaattgtcc acacacactt tctgtttgga gtttctttta catatccctc
     1021 ttcatcagaa agtttgactg tatctttaga aggcatacca atcacacatg cacctgtttc
     1081 cttcactttc tgcagaccac gtttaagaat ttcttctgtc acaaaaggtc ttgcaccgtc
     1141 atggatcaat acatattcac agtctttgca ttcacaaaga cctgcataga cagaatcata
     1201 gcgttctttt cctcccggga ttactgccga tacctttgta aatccatatt tttgcacgat
     1261 ttcttcttta cagtaggaga tagattcctc tcctgtcaca agaataatat cctgaatcag
     1321 cggactttcc tgaaaacagc gcagggaata atacagaacc gggtatccct gtatctccag
     1381 gaactgcttc tgtactttac tgtgcattct ttttccctgc cctgctgcca gaacaatggc
     1441 cgtatttctt tctgacatct caacgctctc ccagtttaag attcggcaca gcattcagat
     1501 cccagccatg acgtgtacct ttaatatatt cataataagc agctactccg atcattgctg
     1561 cattgtctgt acagaagatc ggtgacggcc gataaaaact gtaattattt ttcttacatg
     1621 cttcttccat tgccgctctt aacgttccat tcgaagccac tccgcctgca attgcaatct
     1681 tagtcatccc ataatctttg gctgcaagca ttgtatgctc caccagcaca tcaactaccg
     1741 ctttctggaa agatgctgcc agatctgctc tgtttatctc ttctccggtc atctgcgcat
     1801 gattaatata attcaacact gctgatttta caccactgaa gctgaaatcg tacggacaat
     1861 ctccaatttt agcttttgga aactcaattg cattgggatt tccctcttta gagagcttgt
     1921 ctacctttgg tcctcccgga tatcccagtc cgatagctct tgcaactttg tcaaaagctt
     1981 cgcctgcagc atcatcacgg gtacgtccca ggatttcaaa ttctccataa tccttaacga
     2041 tcacaaggtg tgtatgtcct cctgacacga tcagacataa aaatggaggt tcaagatccg
     2101 gatgttcaat ataatttgct gacacatggc cctcaatatg gtggacccct accagtggaa
     2161 gcttctttgc ataagcaatt gccttcgcct cagcaacgcc cacaagaagt gcacctacaa
     2221 gtcccggacc ataagtgaca ccaattgcat ccagatcatc aagagttaca tctgcatctg
     2281 ccagtgcctg ttcaattacc tggttaatct tttcaatatg tttacgggaa gcaatttccg
     2341 gaactacccc accgtataaa gtatgaagag cgatctgtga agagatcaca tttgacagaa
     2401 tagttcttcc gtttttgaca acagaagctg cagtctcatc acaggaactc tcaattgcaa
     2461 gaattaatgt atctttcatt ttctgttcct catatatccg acggataagt catccgtttt
     2521 actacttgtt cataatcagt ttcagtgaca tattattcct caaaattcaa ttccagactc
     2581 tttccgtctt ttcccggata tgcatttgga aaaatttcac gaactttatc cataaactca
     2641 tccggtctga ccagagacgg actgtaatgt gtcagccaca tctcctttac ccgagcatca
     2701 cgggccaaaa ccgccgcctc gcggaaagtc atatgcttgt atccgcgtgc tttgtctgct
     2761 ttgtcatctt ctccgtacat tccttcacag ataaacagat ccgattcctt tgcatttcgc
     2821 aggatagatt ctgttggtct ggtatctgtt gtataagtaa gcctgattcc ttttctcgca
     2881 ggtccaagta ccatctccgg tgtataggta ataccgtctg cttcgatggt ctgccctttc
     2941 tggagcggat tccagtattt cagtggaata tcctgctctt tcgctctttc agccgagaat
     3001 tttccctggc ggagaatctc aagggtatag ccataacaga gtacattatg gttcacttta
     3061 aaagccgtaa tacgatagcc atttaattca atgacctgct ccggctgtgt aatctcaata
     3121 aatttaattt caaacggaag ctccggtgca atgacgcgaa gtgcattgac cactctctcc
     3181 agacctttgg gacctaccag agtcaatggt tctgtgcggt ctgcatttcc catagtaaga
     3241 agaagtcccg gaagcccgct gatatgatct ccatgataat gagtaaaaca aatcacatca
     3301 ataggtttaa agctccatcc cttctcctta attgctacct gagtaccttc accacagtca
     3361 atgagcagac tgctcccgtt atatcttgtc ataagcgcag tcaaccatct gcgaggcaga
     3421 ggcatcattc caccggttcc aactaaacat acatctaaca tatattccct ctattttcca
     3481 gcttccggca attcccttaa aaggtgtatg gtccggctgc tgattttttc tgctttcatt
     3541 tctttttcat tcgggatgtc tggtcataag cagcgcatcc tccacaggat cactgtaata
     3601 tgccttacgt attccatctc cggtaaatcc catacgttca tagagacgga ttgcagtctc
     3661 atttcccaca cgtacttcca gatggattgt cgtaacctcc ctctctgctg caaggcggat
     3721 caggctctgc atcagaaaag cgccaattcc ttctttttgt ctgtccctgc gcacagccac
     3781 attggtaata tctccctcat ccagaaccat aagaagaccg cagtaaccca gaatcttttc
     3841 tttctcttcc accaccagaa acatggcatc atccctggtc agaaaagtaa aaaaaccttc
     3901 ttttgtccat ggcacatgaa aaagatcctg ctcaatctcc attacctgat ccaggtcatc
     3961 cacaagcatt tcccgcagga tcatattttt gtacctgccg gtttctgctc tttttctctc
     4021 tctgcacgct cacgctctgc ctgggaaacg cggagatatt ccgggatatg ttccatagca
     4081 gtctctgtgc gtccttcttt ataataaata ctgccaagag ctgccactgc cgctgctctc
     4141 tgtttattta catgagccgg tgcaaaagaa tacggaacct gcagtttctc tgcgatcagt
     4201 tcacgaaata caggtacacc gtctcccaaa aatgtaacca tctctcctct ctgattcagt
     4261 tcttccagca gttcctctat cggtgctgcc cactgttctt tcaaagtcat aagctgatgc
     4321 tcttcaaatc tgtaaattcc tgtatatacc tgctttcttc tggcatccat gatcggacag
     4381 atcaatccct gtgcatcata aagattatat gccagtgcat ctactgtcgg tactgctacc
     4441 aatggtttct tcagggcaag tcccaggcct ttggctgttg ccgagccgat acggagtcct
     4501 gtaaaggatc ccggtcccgc tgccactgca atagcatcta cggattccag ttccagttcc
     4561 gtcatcttca caatctcatc taacatcgga agcaaagtct gagaatgtgt tttcttataa
     4621 tttacagtat actcagccag taatgtatca tcttccacaa ctgctacaga tgcaacaatt
     4681 cctgagctgt ccaaagctaa aattttcatt tctacctcct aactgttctc cggaccaatc
     4741 actgtaatcc ttctataatc aaagcctttc tccagatctt tctctatggt tactttgatc
     4801 agcttttccg gaaggatttc ttcaatcaga tcagcccatt cgatcagaca gattccctgt
     4861 ccgaagaaat aatcgtcata tccgatttct tccatttcct caatgtcacc gatacggtaa
     4921 acatcaaaat gatataaagg aagacgtccg ctttcgtatt cctgaatgat cgtgaatgtc
     4981 gggctgcaga taggctctgt gattccaagc cctgccgcaa ctccctgggt aaatacggtt
     5041 tttccaactc caagatcacc tgtcagagta taaatctgac ccggtttcgc attctctccg
     5101 atcttctttc ccacctcaaa tgtttcctgt ggtgtttttg tctctataat catcttttcc
     5161 acctttttct tgcagaattg ttctttgcat attataatag agcgaacagc gaaatacaat
     5221 actttttgaa aaggagactt gaaactatgg caaatccgat agttacaatt accatggata
     5281 atggcgatgt gatgaaagca gaattatatc ctgagattgc accgaataca gttaataact
     5341 ttatcagtct tgtaaaaaaa ggcttctatg acggacttat tttccatcgt gtgatcaacg
     5401 gtttcatgat ccagggagga tgcccggacg gaacaggtat gggcggtccg ggatacagca
     5461 ttaagggtga attcactcag aacagattta aaaatgatct gaaacatact gcaggtgttc
     5521 tttccatggc acgcgccatg catccaaact cagcaggaag tcagttcttt atcatgcaca
     5581 aagatgctcc gcatcttgac ggtgcttatg ctgcattcgg caagatcact gaaggaatgg
     5641 atgttgtaaa ccgtattgca gaagaggata ccgattacag cgaccgtccg cttgacgagc
     5701 agaaaatcaa atccatgaca gtagaaactt tcggtgttga ttatccggaa ccggagaaat
     5761 gctgattcgc tgactgttta tgccccatgc agttttcagg ctgcatgggg catattttat
     5821 cagtccgtaa atactttcac ctcaccgtca atcactgaat agtaaagatc tttctctttc
     5881 ttcatcttcg ctcttccatt tgttccctcg gtgattgctt tttctacaaa tgcaatctga
     5941 tccaccggaa ccagcaaatg cagaactacc ctgtcagtgt attcagaatc aaggatcggt
     6001 aatttctgtt ctccggcaat atactggatc ttgcctatcc cggtataatc cgtaactact
     6061 tccatagaaa ttccgtaggt tttctcaatc actgtgctgg cggcaagacc ttcctgcaga
     6121 cttctggaat atgctctgac aagacctcct gttccaagaa gtatccctcc aaaatatctg
     6181 gtaactactg ctgccacatt ataaatatct tcccccagga ttacatccag cattggtctt
     6241 cccgcagttc ctgacggctc accatcgtca ctgcatctgg tatattccct gttctttccc
     6301 accgaatata catagcagtt atgtctggca tcccaatatt tcttcttcat ttcctctata
     6361 aaggccagtg cctcctcttc ggtctgtaca ggacgcactg tagcaataaa tctggatttc
     6421 ttttccacga tctcgccttc cccgccctcg taaactgtct tatatttctc aagcatgtct
     6481 catactcctc tttccgttct cttattgact cagtgtacca tgaattacaa aaatgtgcca
     6541 gcacagtgta tactaatcct gtaaaaaaac acaataacca ttaaaaagat ccaaaagcaa
     6601 ttgtgaattt ttatttttct gctttattta cttattctta tttgatataa tagaacggat
     6661 tataaaggag gtttaacatg aactacggta aaaagtcgac tgcaaagaaa cggacggcac
     6721 tgatttcccg ttcttccatg atgggaaagc gcgccagagt ttctttcatc agagtcctgt
     6781 tcgtatcact gatagcactc tgcatcgcag taacctgtct gggtgtgggg tcattcaggg
     6841 gtgtaattga tactgctcct gatgttgatg atattgatat tatgccactg ggctatgcaa
     6901 cctttctcta cgatgatgca gggaatcaga tccgtaaact ggctgctccg gattccaacc
     6961 gactgcctgt cactctcgat cagatacctg ttgatctgca gcatgcagtt gtcgcaattg
     7021 aggatgaacg tttctatgaa cataacggta ttgacgtaaa aggtattctt cgtgccggaa
     7081 tgaaagctct cactaccgga gatttctcag aaggtgccag tacgatcaca cagcagcttc
     7141 ttaagaataa cgtatttacc aactggacca gtgaatctac tcagctggaa agatttacgc
     7201 gaaaaatcca ggaacaatac cttgctgtcc aggttgagaa aaaaactgat aaagacacca
     7261 ttcttgagaa ttacctgaac acaattaacc tgggtgccgg ttcctacggt gtacaggctg
     7321 ccgcacgaca gtattttgac aaggatgtat gggatctgaa tctttctgaa tgtgcaactc
     7381 tggcaggtat cacacagaac ccgacgaaat ttaacccgat catcaatcct gacagcaaca
     7441 ggaagagacg caaggaagtt ctccagcata tgctggatca gaactatatt actcaggatc
     7501 agtatgatga aactcttgca gatgatgttt attcccgtat ccaggccgct caggaaaaga
     7561 actccagcac tgagaatact gtttacacat attttgaaga tgaactgaca gatcagatca
     7621 tcaacgacct gatgaatatc aaaggttaca ccaaaaaaca ggcaaccaat cttctctaca
     7681 gcggcggact taaagtatat acaacacagg attccaagat ccagaatatc ctggatgagg
     7741 aatatgcaga tccttccaat tatccggaca ctgtccagta tgaacttgac tatgccctga
     7801 cggttaccga tcctgatgga aatcaggtaa actacagtaa ggaaatgctg cagctttatt
     7861 ttcagaatga ggatcccgat ttcgatcttc tgttcgattc cccggaagat ggacagactt
     7921 atgtagacaa atataaggca agcatactcg ccaacggaag taaagttctt gcagagcgtg
     7981 tcaatttcgc acctcagcca cagtcttcca tgagtgtgat cgaccagcat accggctatg
     8041 taaaagctct gatcggtggt cgtggagaga aaacggcaag tcttacactg aaccgggcaa
     8101 cggacactac cagacagcct ggttctacct ttaagatcgt gtccacttat gcacctgctt
     8161 taaatgaaaa aggcatgact cttgcaacaa catttgaaga cgaaccttat gaatatccgg
     8221 atggttcccc tgtaaacaac gcaacccgtt cttacaacgg aacaaccacg atccgtactg
     8281 cgatccagaa ttccattaac gttgtagccg tcaagtgtct ggaaaaagta actcctgagc
     8341 ttggattgaa atatctggac aactttggtt ttacaaccct tgctcatgga acagaggctg
     8401 acaaagatgc caatggtaat gtatggagtg atgcaaacct tgccactgcg cttggcggca
     8461 tcacccgcgg tgtcacaaat gttgaactgt gtgcatccta tgcagccatc gcaaataacg
     8521 gaaattatat caagccaatt tattacacaa aaattctgga tcacaacgga aacgtcctga
     8581 tcgagaatac tgctgccgaa cgttccgtaa tcaaagaaag tactgctttt cttctcacca
     8641 gtgcaatgga ggatgttgta aaacagggta caggtacagc ctgccagctt gacaatatgc
     8701 ctgttgccgg aaaaacaggt acaaccgaag cctataacga cttgtggttt gtgggttata
     8761 caccatatta tacatgcgca gtatggtccg gttacgacaa caatgagaaa ttacctgatt
     8821 atgcgcgtaa cttccacaaa gctttatgga aaaaggttat gactcgtatc catgagggac
     8881 ttccttccaa agaattcgag aagcctgcaa gtgtagaaaa gttaagtgtc tgcgaggaaa
     8941 ccggacttct tccaagagcc ggctgtccgg tgatcactga atactttgac ataggtacca
     9001 tgcctactga atactgtgac cagcatttct atgacagtga tgattatgac tataattatg
     9061 atacagattc ctctgatcag actgacaaca ccactgacac tgataacagc gaaaactctg
     9121 ataatggaga taccgacaac agtggtgaca gtaacaatac tgatgacaac ggtaacagcg
     9181 gtgacgatgg tactgataat accggcggaa gcgatgacaa cggtgacggt aacgaggatg
     9241 attcctctta tcaggtcgac tattattaat gcaaaaatac cggtacagga gcacctccga
     9301 atgatgttcc tgtactgcaa aaaatccaca gtcctgtcaa ctcaggactg tggatttttt
     9361 atatattttc aaaattgatt ctcagatatt atacttcttt aataaccata cgcgcagatc
     9421 cgtaaatacc tgcgtcattt ccgagagttg caagaatgat cggtgttttc ttgcatgctg
     9481 taaaagcata tttctcatag tatttctgaa tacagtcgat caatggctgt ccggctttgg
     9541 aaacaccacc gccgataacg atggcttccg gatctgtcac acatgcaaac atggcaagtg
     9601 ttccgccaag catttctcct acctgtgcca taacatcgca ggctactttg tcgccctctt
     9661 taaaagcatc cagaacattc tttgctgaaa gttttgtaaa tttgcgaaga acagtctgct
     9721 catctgtagc agccagagtt ctctgtgcca cgcgaacgat acctgttgca gacgcatact
     9781 gctccagaca tccacagtta ccacagttgc aggcttcctt ttcttcatgg tcaactgctg
     9841 catgaccgat ttctccacct gcaccatgag caccgccaac aatctttcca tcaacgatga
     9901 ttccaccgcc gacacctgtt ccaagagtta caagaataac gttcttagct cctgctgctg
     9961 cgcctttcca tgcttcgcca agtgctgcta cattggcatc gtttcctgct ttggacggaa
    10021 gtcctgtcag ttctgtcagt tccttagtaa cttctttaaa gccccagaaa aggtttaccg
    10081 cacatggcac ttcccctctc tcgttaacag gt
//
LOCUS       DBMDXG010000026        10091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1850_length_10091_cov_1.913714, whole genome shotgun sequence.
ACCESSION   DBMDXG010000026 DBMDXG010000000
VERSION     DBMDXG010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1850_length_10091_cov_1.913714"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(41..997)
                     /locus_tag="ACZPD3_01650"
     CDS             complement(41..997)
                     /locus_tag="ACZPD3_01650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1414746.1"
                     /translation="MQEVSVIIPNFNGMAYLDGVLAGLECQTVSNFDVILVDNGSNDG
                     SCAFVAARYPWVHLIELPENFGFCKAVNEGIRASRSPYVLLLNNDIEVTENFIEEMLS
                     AIKRHPKAFSCAARMIQFHDRDRLDDAGNYYCALGWAYARGKGKNIHTYEKEEKIFAS
                     CAGAAIYRKKIFDELGYFDEEHFAYLEDMDVGYRARIYGYENWYAPDAMVYHVGSGTS
                     GSRYNQFKIRYSSRNNIYLIYKNMPVLQIIINLPFLVAGFGIKILFFTLKGFGREYIA
                     GIKNGFQISKKNHKIPFKFQNLSNYFGIQVELWVNIFRRLCG"
     gene            complement(979..2886)
                     /locus_tag="ACZPD3_01655"
     CDS             complement(979..2886)
                     /locus_tag="ACZPD3_01655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1414747.1"
                     /translation="MSKKPSAGGKIQWTKMFRKLSPYTIRKGFRYMKHYGPKEFWIRL
                     HERFEPEEVPYGPWYRAYIPTEETLETQRKQKFDYSPLISIAVPAYQTPVEFLRQMIE
                     SLIVQTYSNWELCIVNASPDNEEMQKVLAEYSAGDSRVRFCNLKENLGIAENTNRAFA
                     MAKGEFVGLLDHDDLLAPNALYEIVKILQDHPQADALYTDEDKVTTELDEHFQPHLKP
                     DFNLDLLRSNNYICHFFVVRKSIVEKAGGFRKEFDGAQDYDFIFRCTENAGEVLHVPE
                     ILYHWRTHKASTADNPASKMYAFEAGKRAIEAHLERTGTEGEVSHTQDLGFYRVKYPV
                     QGKPLVSVIIPNKDEKETLQTCLEMLEKNTGYQNFEIIIVENNSTTDEIFRYYKELSG
                     NRKIHLLRWGKEFNYSAINNFAAAHAKGEYLLFLNNDVKSINPDWLEEMLGVCQRPEV
                     GGVGAKLIYPDNTIQHAGCVIGMGGIAGHMFVDMPADHTGYLHKASLLQDMSAVTAAC
                     LLMKKEVFEQAGGFTEELAVAFNDVDLCLKVRKNGYLIVYDPYAKLYHMESKTRGAED
                     SKEKVRRFQTEIEYMRCHWIDILKNGDPCYNKNLSLTKWNYSLKPIPGMETEAGQKKE
                     KTGRKSCRKYQ"
     gene            3202..4482
                     /locus_tag="ACZPD3_01660"
     CDS             3202..4482
                     /locus_tag="ACZPD3_01660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1414748.1"
                     /translation="MMIKKKRITAALMALGLGAVTMFSQFPVSAAEETAQNTDAAAQT
                     ADPSVVVTNGIDGWPQASDISSAAAIVMETSTNTVLYSKNADQPLYPASAVKIMTCLV
                     ALENSSLDEQVTMTATGVSGVTDGGANISSQLDEVFTMEQCLYAIMVASANDIALQVA
                     EHVGGSVDAFVQIMNTRAQELGCTNTVFTNPTGLPDENQHITAHDMALIMEAAMANDT
                     FRTIAATTSYTLPATNVSGGERVLTNNFTMINSTSDGYYKPCIGGKEGYTEASGSTLV
                     CEASKNNMKLVCIVLNGASGVTDNEAIALLNYGFDNFAPLTIADDDFNRLSGGTVIAP
                     NGATEDNLTTEDTSSDGQITRQYYFGGTPVGTAILEDAEQQTNDAAVTGQKNMEAAQA
                     YSASHTTAPYYIIGAIGAAFLLFFLFLMVKVIKS"
     gene            complement(4589..6049)
                     /locus_tag="ACZPD3_01665"
     CDS             complement(4589..6049)
                     /locus_tag="ACZPD3_01665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR1414749.1"
                     /translation="MNVKEDMLKKKKEINEKTEIFIFVFLAFILLTTWAMTQPFNSGP
                     DEQMRYYVADYIYKHHGALPGGDDPAVRNKVWGISYAYYPVVSYMVSALFMRISRLFA
                     DPGYSMFKIARMADVLFVTGAVYFVVKASGKLFPKEKYSREVRWLFAALAGFMPQAIF
                     MGTYVNTDSLALLAAAMILYAWVSYLREDWTWKNCILLAVGMAVCALSYYNAYGWILC
                     SFFFFCFTVLLCREEAFSQRVRFLFSRGAVIAAVTLVLCGWWFIRNAVLYNGDFLGRK
                     SCAECAEKYAQKDYRPSLYPTPAKLGWNWKDIILYQDPGWYHNWILTVCVSFIGTFGQ
                     MEIYMPYTVSKLYMLFFAVGIISVFFVKETFDLRKKMYVAQRKAVGNDRWKIKTKVIS
                     REWNKEGIFHLMMVFLIMIPVFLFLYYVYYSDNQPQGRYLMPALYPLMYFVTLGWNNI
                     LTKTVKNEKLRSLIYRVLTVLLVISPFACWAFLILS"
     gene            complement(6068..8512)
                     /locus_tag="ACZPD3_01670"
     CDS             complement(6068..8512)
                     /locus_tag="ACZPD3_01670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1414750.1"
                     /translation="MPKEIFEVVRERFHLSDRTQYIVQGHWPKDAVMEAYLDQHKLKV
                     SVKENENVSALERFKDPEKMQGIQITATVQIPENLEGYHKLVIYEKFPDKKHVWFSIT
                     AGELDKKRDKPQVYFEEESAEQGTVRIRGWAIAPKPVTVRIFDADKKPVAAEIQRTDR
                     VDVNQLFEEAQDPGKTGFFSEITNVSGKCLYVVFYAGEKKTVHVVPLRKADILAKKLD
                     KYVEKGIRYWKSQGAAALAEKVVTKVKNVRQGPPSYQKWIRHHLPDRNELEKQKKTSF
                     GYRPKISFVVPLYKTPEKYLRRLTESFQEQTYSNWELCFSDGSGAQSPLTELLKELTA
                     KDNRIKYVSHEEPLQISENTNSAIEIATGDFIAFADHDDELTPNALFECVKAINEKPQ
                     TLVIYTDEDKMSMDGHKFFQPHFKPDYNPDLLCTVNYICHLFVVSRKVIEKVGGLRSE
                     FDGAQDYDFVLRCVEAVKDEEICHIPKILYHWRCHEDSTAENPESKLYAFEAGRRAVQ
                     AHYERTGIHAEVFKGEYLGLYRTKFIRDHDPLISIIIPNKDHIDDLKRCMESIEQKST
                     YKNYEYIIVENNSTDSATFEYYKKLEAENPKVRMVYWDGVFNYSAINNYGASFAKGEY
                     LLLLNNDTEIINPDCLEELLGYCMRKDVGAVGARLYYEDDTIQHAGVVIGFGGIAGHC
                     FVQQKRGTTGYCHRIICAQDYSAVTAACMMVKKSAFDAVGGLSEELAVAFNDIDFCMK
                     LRKAGYLIVYNPYAELYHYESKSRGLEDTPEKVARFNKEIATFEKKWPEILKNGDPYY
                     NPNLTLKSQDFSLKRI"
     gene            complement(8514..>10091)
                     /locus_tag="ACZPD3_01675"
     CDS             complement(8514..>10091)
                     /locus_tag="ACZPD3_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1414751.1"
                     /translation="WNGFTMTGREDDYTWTEPYMDNSQVFVADACEGEETGEFLRKKY
                     KKEIRLSYKKVTENNGISGNLNASLKLAMGEYVLFAGQEIIPEPDALFQMVKAITEKK
                     ADMIYTDEDEISADGKHYSEPEFKPDFNLFRLRENNYIGQFWAIRKEILEQAGKFDPE
                     YDGAQDYDMLLRCSEQAENIVHIPKILCHSMKAENLITEEQEKKNWEAGRKALEEHYR
                     RAEVSATAELADKKGWYRSHLTISGEPMISVIIPSKDHINDLELCISSIEEKTTWKNY
                     EIIIVENNSVEKETFVYYETLKNRYPNVRILTWKKEFNYSAINNFAVREAQGEYLLFL
                     NNDVEIITENWLEEMLQLCQQKDVGMVGAKLYYPDDTIQHAGVVVGLGGVAAHVLCKL
                     SRDAEGYMGRLRCVQEISAVTAACMMVKTSVFKAVGGFDEELKVAFNDIDLCMKVRKY
                     GVKIVFTPYAELYHYESKSRGMEDTPEKQLRFSREVNCFRRKWERELLKGDPYYNPNL
                     TLNNTDCSLRKQEKNGD"
BASE COUNT         2691 a         2473 c         1821 g         3106 t
ORIGIN      
        1 ttgttttata aaagcaataa aaatttatct ttctttctta tcatccgcac aaacgcctga
       61 agatattcac ccacaattca acttgaatcc caaaatagtt tgacagattt tgaaatttaa
      121 aaggtatttt atgatttttt ttgctgatct ggaatccatt cttaattccc gcaatatact
      181 ctcttccgaa tcctttcagc gtaaaaaaca gaattttgat tccaaatcct gcaacaagaa
      241 acggaagatt gataataatc tgtaacaccg gcatattttt gtatatcaga taaatattat
      301 ttctggaaga ataccggatc ttgaactgat tatatctgga tccgctggta ccgcttccca
      361 catggtatac catggcatca ggtgcatacc agttttcata tccgtagatc ctggcccggt
      421 atcccacgtc catgtcctcc agatatgcaa aatgttcttc gtcaaaatat cccagctcat
      481 caaagatctt ctttctgtaa atagcagctc ctgcacagga agcaaaaatc ttctcttcct
      541 tttcataggt atggatattt ttccccttac ctcttgcata agcccatcca agcgcgcagt
      601 aatagtttcc tgcatcatcc agcctgtccc tgtcatgaaa ctgaatcatt ctggcagcac
      661 aggagaaagc tttgggatgc cttttgatgg ctgacagcat ttcttctata aaattctccg
      721 ttacttcaat atcattgttt aaaagcagta cataaggtga acgtgatgct ctgatccctt
      781 cattgaccgc tttacagaat ccgaaattct ccggcagttc gatcagatgg acccatggat
      841 accttgcagc aacaaaagcg cagcttccgt cattggatcc attatccaca agaattacat
      901 caaaattgct tacagtctga cactccagac ctgcaagtac gccatccaga taagccatcc
      961 cattaaaatt cggaatgatc actgatactt cctgcatgat ttcctccctg tcttctcttt
     1021 tttctgtcct gcttctgtct ccattcccgg gattggcttc agagaataat tccatttggt
     1081 aagagacagg tttttgttat aacagggatc tccgtttttg agaatgtcta tccagtgaca
     1141 tcgcatatat tcaatctccg tctgaaaacg gcgcactttc tccttgctgt cttcagctcc
     1201 tctggtcttt gattccatat gataaagctt cgcataggga tcatatacga tcagatagcc
     1261 gttttttctt actttcaggc aaagatccac atcattaaat gccacagcca gttcttctgt
     1321 aaatcctcct gcctgctcaa aaacctcttt cttcatcaac agacaggcgg cagtcactgc
     1381 actcatatcc tgaagaaggg atgccttatg cagatagccg gtatggtccg caggcatatc
     1441 tacgaacata tgccccgcaa taccgcccat tccgatcaca catcctgcat gctggattgt
     1501 attatccgga taaatgagct ttgctccgac accgcccact tccggacgct gacacactcc
     1561 aagcatttct tccagccagt ccggattgat ggattttaca tcattattca ggaaaagcag
     1621 atattctccc tttgcatgtg ccgcagcaaa gttattgatg gcagaataat taaattcttt
     1681 tccccatctc aaaagatgga tcttccggtt tccggaaagc tctttataat atcggaaaat
     1741 ctcatcagtg gtactgttat tctccacgat tatgatttca aaattctgat aaccggtatt
     1801 cttctccagc atttccagac aggtctgaag agtctctttc tcgtctttgt ttggtatgat
     1861 aacagacacc agaggttttc cctgcaccgg atatttcacc cggtaaaagc ccagatcctg
     1921 tgtatggctt acctcgcctt ctgtacctgt tctctcaaga tgagcttcta ttgctctctt
     1981 gccggcttca aatgcgtaca ttttactggc aggattatct gcagtggagg ctttgtgtgt
     2041 tctccagtga tacaggattt ccggaacatg taaaacctct ccggcattct ccgtacagcg
     2101 gaagataaaa tcgtaatcct gagctccgtc gaattctttt cggaaacctc cggccttttc
     2161 tacaatactc tttcgcacta caaaaaagtg gcagatataa ttattggaac gcaggagatc
     2221 cagattaaaa tccggtttca gatgtggctg aaaatgctcg tccagttctg ttgtaacctt
     2281 atcctcgtct gtataaagcg catctgcctg cggatgatcc tgcagtattt tcacgatctc
     2341 atataatgca ttgggtgcca gcaggtcatc atggtcaaga agccccacaa attcaccttt
     2401 tgccattgca aacgcgcggt ttgtgttctc tgcaatgcca aggttctctt taagattgca
     2461 gaatcttacc cgggaatctc cggctgaata ttctgccagt actttctgca tctcctcatt
     2521 gtccggactt gcatttacga tacacagctc ccagtttgaa taagtctgaa cgatcaatga
     2581 ttctatcatc tgtctcaaaa attctacagg tgtctgataa gccggaactg caatgctgat
     2641 cagcggtgaa taatcaaatt tctgtttcct ctgtgtttcc agtgtttcct ccgtcggaat
     2701 gtaagcccgg taccatggac catatggtac ttcttccggt tcaaaacgtt catgcagcct
     2761 gatccagaat tcctttggtc cataatgctt catgtagcgg aaccctttcc ggatcgtata
     2821 gggggaaagc ttcctgaaca ttttcgtcca ctggattttt cctcctgcag atggtttttt
     2881 actcatatga taacctcgat ttttactatg attttttatt ttaacattgt atgatatttt
     2941 ttgcaagaaa ggagagaaat gtgttatact gttattgttt ttatggttac tgttcacaca
     3001 ccactatccc gcgaggtgtt ttggcatgaa tatgccaaaa tcccgagaca tacaagtcaa
     3061 aattccattg ccgcaggcaa tctggaatga attttggcta agttactgtg aacggagtga
     3121 acagttactt tttatggtta ctgttcactt acaatgaaca gttacttttc aatattttta
     3181 tacacaggag tattatatac catgatgata aagaaaaaaa gaatcactgc agccctgatg
     3241 gctctgggac ttggtgctgt caccatgttt tcacagtttc cggtatctgc tgctgaagaa
     3301 acagctcaga atacagacgc cgccgcacag acagcagatc cgtctgtcgt agtcaccaac
     3361 ggtatcgacg gatggcccca ggcttccgat atttcgtctg ccgcggcaat tgtaatggaa
     3421 acttccacaa acacagtcct gtattccaaa aatgcagatc agcctcttta ccctgcaagc
     3481 gcagtcaaga ttatgacctg cctggttgct cttgagaaca gcagtctgga tgaacaggta
     3541 accatgactg caaccggtgt ttccggtgtt actgacggag gagccaatat ttcctcacag
     3601 ttagatgagg ttttcaccat ggaacaatgt ttatatgcca tcatggttgc ctctgccaat
     3661 gatatcgcac tgcaggtagc tgaacatgtg ggaggttctg tggatgcttt tgtacagata
     3721 atgaataccc gggcacagga acttggctgc accaatactg tctttaccaa tcctaccggc
     3781 cttcctgatg agaaccagca tatcaccgcc catgatatgg ctctgatcat ggaagcagct
     3841 atggcaaatg acactttccg caccattgca gcaactactt cctatacact tccggcaaca
     3901 aatgtttccg gcggcgaacg tgtacttaca aacaatttta ccatgataaa cagcaccagc
     3961 gacggttatt ataaaccctg tatcggcggt aaggaagggt acacagaagc ttccggaagt
     4021 accctggtat gtgaagcttc caaaaataac atgaagctgg tatgcattgt attaaatggc
     4081 gcatccggtg ttaccgataa tgaagcgatc gcgcttctca attatggttt tgacaatttt
     4141 gcacccctta ccatcgcaga cgatgatttt aaccgtcttt ccggtggtac agtcattgcg
     4201 ccaaacggtg ccacagaaga taatctgacc acagaagata cttcttctga cggacagatc
     4261 acacgccagt attactttgg aggaactcct gtaggaactg cgattctgga agacgcagag
     4321 cagcagacaa atgatgccgc agtaaccggt cagaagaata tggaggcagc acaggcatat
     4381 tctgcttccc atacaactgc tccttattat atcatcgggg cgatcggggc agcattcctg
     4441 ctgtttttcc tgttcctgat ggtcaaagtg atcaaatcct gaagcctgct tctgaatacc
     4501 agacagtaac catttctttg atagataaaa tagataaatt taagggactt ccgcaaaagc
     4561 ttttcatgcg gcagtccctt tatttctttt aagacaagat taaaaatgcc cagcaggcaa
     4621 atggcgaaat caccagcagt acagtcagta ctctgtatat cagactccgc aacttttcat
     4681 ttttcactgt ttttgtcaga atattattcc agcccagcgt aacaaagtac atcagaggat
     4741 ataatgcagg cataagatat cttccctgtg gctgattatc actgtaataa acataataca
     4801 gaaacagaaa taccggaatc ataatcagaa ataccatcat caaatggaaa ataccttctt
     4861 tattccactc tcgggatatc acctttgttt taatcttcca tctgtcattt ccaactgctt
     4921 tccgctgtgc aacatacatt ttttttctca gatcaaatgt ctcttttaca aaaaatacac
     4981 tgatgatccc aacagcaaag aacagcatat acagtttgct gaccgtatat ggcatataaa
     5041 tttccatctg cccaaaagta ccgatgaaac ttacacacac tgtcagaatc cagttatgat
     5101 accagcccgg atcctgataa aggataatat ctttccagtt ccatcccagc tttgcaggcg
     5161 tcggatacag tgaaggtctg taatccttct gagcatattt ttccgcacat tccgcacagg
     5221 atttccttcc caggaaatct ccgttataca aaactgcgtt acggatgaac caccagccgc
     5281 agagaactaa agtaactgct gcgatcactg ctcctctgct gaaaaggaaa cgaactctct
     5341 gtgaaaaagc ttcttctctg cagagtaata ccgtaaaaca gaagaagaaa aaactgcaga
     5401 gtatccaacc gtatgcatta taataagaca atgcacatac ggccattccc actgcaagga
     5461 gtatacagtt tttccatgtc cagtcttctc tcagataaga gacccatgca tacaagatca
     5521 tggctgctgc cagcagtgcc agtgagtctg tattcacata agttcccata aagattgcct
     5581 gcggcataaa tcctgccagt gctgcaaaca gccatctaac ttctctggaa tatttttctt
     5641 tcggaaacag tttgcccgaa gcttttacaa caaaatacac tgcacctgtg acaaacagta
     5701 catctgccat acgtgcaatt ttgaacatgg agtatcccgg atctgcaaaa agccgggata
     5761 ttctcataaa taatgcagac accatataag atacaaccgg ataatatgca taggaaattc
     5821 cccacacttt attcctgaca gccggatcat ctccccccgg aagagctcca tggtgtttat
     5881 aaatgtaatc tgcaacataa tatctcatct gttcatccgg ccctgaatta aaaggctgtg
     5941 tcatcgccca ggttgtcagc aggataaacg ccagaaatac aaaaatgaaa atttctgttt
     6001 tttcattgat ctctttcttt ttctttaaca tatcttcttt cacattcatt ttatagcaat
     6061 ttcatcttca gattcttttc agagaaaagt cctggctctt cagagtgaga ttggggttat
     6121 aataaggatc tccgttcttc aggatctccg gccatttttt ctcaaaggtg gcaatttctt
     6181 tgttaaatcg tgccactttt tccggagtat cctcaagacc tctggatttg gattcataat
     6241 ggtataattc cgcatatgga ttatacacga tcagatatcc cgcctttctc agcttcatgc
     6301 agaaatcaat atcattaaat gcaacagcca gttcctctga aagtcctcca actgcatcaa
     6361 atgcagattt cttcaccatc atgcaggcag ctgtaaccgc actgtaatcc tgtgcacaga
     6421 taattctgtg acaatatccg gtggtacctc tcttctgctg gacaaaacag tgtcctgcaa
     6481 ttccgccaaa accgatcaca acacctgcat gctggatcgt atcatcctca taataaagtc
     6541 ttgcacccac tgcgcctaca tctttacgca tgcaataacc caggagttct tccagacagt
     6601 ccggattaat gatctctgta tcattgttaa gcagaagcag atactcaccc tttgcaaaag
     6661 atgcaccata attattaatg gcagaatagt taaagactcc gtcccagtat accatgcgga
     6721 ccttcggatt ctctgcctcc agttttttgt aatattcaaa agtagcagaa tctgtactgt
     6781 tattctctac aataatgtac tcataatttt tgtacgtaga tttctgttca atggattcca
     6841 tacatctctt cagatcatca atatggtctt tattcggaat aataatagaa atcagcggat
     6901 catgatctct gataaacttc gttctgtaca gccccagata ttcaccctta aaaacctctg
     6961 cgtggattcc tgttctctca tagtgagcct gtactgcacg tcttccggct tcaaaggcat
     7021 aaagcttgct ctcgggattc tctgctgtgg agtcctcatg gcaacgccag tgatacagga
     7081 tttttggaat atgacagatt tcttcatcct ttactgcttc cacacagcgt aacacaaaat
     7141 cataatcctg tgcaccgtca aactcacttc gcagcccgcc aactttttca attacttttc
     7201 gtgataccac aaacagatgg cagatataat tgaccgtgca cagcagatcc ggattatagt
     7261 ccggtttaaa atgcggctga aagaatttat gtccatccat agacatctta tcttcatctg
     7321 tgtagatcac cagtgtctgc ggtttctcat ttattgcttt cacacattca aataatgcat
     7381 tcggcgtcag ctcatcatca tggtctgcaa aggcaatgaa atcacctgtt gcaatttcaa
     7441 tggcactgtt ggtattctct gaaatctgaa gcggctcctc atgagaaacg tatttaattc
     7501 tgttatcctt cgctgtcagt tcttttaaca attccgtcag cggtgactgt gcgccgcttc
     7561 cgtcagagaa acaaagttcc cagtttgaat aagtctgttc ctgaaatgat tctgtcagtc
     7621 ttctgagata tttctccggt gtcttataca gcggtactac aaaagaaatc ttgggtctgt
     7681 atccaaagga tgtttttttc tgtttctcca gttcgttcct gtccggcaga tgatgtctga
     7741 tccatttctg ataagatggc ggtccctgac gtacattttt aacctttgtc actaccttct
     7801 cagcaagagc cgccgcaccc tgagatttcc agtagcggat tcctttttct acatatttgt
     7861 ctaatttctt tgccagaata tcagcttttc tcagaggaac cacatgtaca gttttcttct
     7921 cgcctgcata aaacactaca tacaggcatt ttcccgaaac gttggtgatc tcagagaaga
     7981 atcctgtctt tcccggatcc tgtgcttctt caaacagctg gttgacatcc accctgtctg
     8041 ttctctggat ttcagcggca accggctttt tatccgcatc aaagatacgg acagtcacag
     8101 gcttcggtgc aatggcccac cctctgatcc gtacagttcc ctgttctgca ctctcttctt
     8161 caaaatatac ctgcggttta tctctttttt tatccagttc accggcagtg atggaaaacc
     8221 agacatgttt tttatccgga aacttctcat agatcacaag cttatgatac ccctccagat
     8281 tttccggtat ctgaacagtt gccgtaatct gtattccctg cattttctcc ggatctttaa
     8341 aacgttccag tgcactcaca ttttcatttt ctttcacaga caccttcagt ttgtgctgat
     8401 ccagatatgc ctccatcacc gcatctttcg gccagtgtcc ctgcacaata tactgtgtcc
     8461 tgtctgataa atgaaatctt tctcttacta cttcaaatat ttccttaggc atgttaatct
     8521 ccgttcttct cctgttttct cagagaacag tctgtgttgt ttaatgtcag attgggatta
     8581 taatagggat ctcctttcag gagctctctt tcccattttc tccggaaaca gtttacttct
     8641 ctggaaaaac gcagctgttt ctccggtgta tcttccattc ctctggattt ggattcataa
     8701 tgatacagtt ctgcatacgg tgtgaataca attttcactc catattttct cactttcata
     8761 cagaggtcaa tatcattaaa tgcaaccttc agctcctcgt caaatcctcc aactgcttta
     8821 aaaacagaag tctttaccat catgcaggcg gcagtgactg cacttatctc ctgcacacag
     8881 cgcagacgtc ccatatatcc ttccgcatcc cgggaaagtt tacataatac atgggcagca
     8941 actccaccaa gaccaactac cactcctgca tgctgaatgg tatcatccgg ataatacagt
     9001 ttcgcaccga ccattcccac gtctttttgc tgacaaagct gaagcatttc ttcaagccag
     9061 ttctctgtaa tgatttccac atcattgtta agaaacagca gatattctcc ctgcgcctct
     9121 cttactgcaa agttattgat cgcagaataa ttgaattctt ttttccatgt aagaatcctt
     9181 acatttgggt atctgttttt cagtgtctca tagtatacaa aggtttcttt ttccacactg
     9241 ttgttctcaa caataatgat ctcgtaattc ttccatgttg tcttctcttc tatggatgaa
     9301 atacacagtt ccagatcatt aatgtgatct ttacttggaa tgatcacaga aatcatcggt
     9361 tcgccggaaa tagtcagatg gcttctgtac catcccttct tgtctgcaag ttccgctgtt
     9421 gcagaaacct ccgccctgcg gtaatgctcc tccagtgcct ttctgcctgc ttcccagttt
     9481 ttcttctcct gttcttctgt aatcaggttc tctgctttca tggaatgaca gaggatcttt
     9541 ggaatatgca caatattttc tgcctgctca ctgcatctta agagcatatc ataatcctgt
     9601 gcaccatcat attccggatc aaactttccg gcctgttcca gaatttcctt ccggattgcc
     9661 cagaactgtc cgatataatt attttccctt aatctgaaca gattgaaatc cggcttgaat
     9721 tccggctcag aataatgttt tccgtctgcg ctgatctcat cttcatctgt atagatcata
     9781 tccgcttttt tctctgtgat cgctttgacc atctgaaaaa gtgcatcagg ttcaggtatg
     9841 atctcctgac cggcaaacag aacatactct cccattgcaa gtttcagaga tgcattaaga
     9901 tttcccgaaa tgccgttgtt ttccgtaacc ttcttgtagg aaaggcggat ctctttttta
     9961 tattttttcc gcagaaactc tcctgtttcc tctccctcac atgcatctgc aacaaacacc
    10021 tggctgttat ccatataagg ctctgtccat gtgtaatcat cttcacgtcc ggtcattgtg
    10081 aacccgttcc a
//
LOCUS       DBMDXG010000027         9970 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1875_length_9970_cov_2.293951, whole genome shotgun sequence.
ACCESSION   DBMDXG010000027 DBMDXG010000000
VERSION     DBMDXG010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_9970_cov_2.293951"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="ACZPD3_01680"
     CDS             <1..568
                     /locus_tag="ACZPD3_01680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414752.1"
                     /translation="DTRDFQIKTVQGLQDFQSLLEQEKYEQADTAIRNLNQTFQKERF
                     HPYCQNNLLQAILEGKRLRAEQKHIQVSYEILLPEKISINTTDLSSIFFNLLDNAIEA
                     CSASGNPDPEIRLSANISNGFLTVYMHNTKNPMQTFTHKTTKSEPGAHGYGLSIIEDI
                     CQKYNGSYQWIDHNNTFDSIVLLQIKNL"
     gene            587..1474
                     /locus_tag="ACZPD3_01685"
     CDS             587..1474
                     /locus_tag="ACZPD3_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1414753.1"
                     /translation="MPVTPTIEVDDTLKERSSHGSFELPIETYTDNCEIFHSLYNHWH
                     DEMELIYIESGSGLVRLNKEILRVKAGDLIIVNRGVLHGIKTDLKNILYFRSIVFDLN
                     FLSGPAGDLCQERVISLLMDNQAEFTHIISRSDDNYGNICLLFDRIHSCHKEKKPYYF
                     VKLKALFFSFFYEMLMGNYITPADKEENKSLASIKKILDYINLNYSQSLTAAELTALS
                     NYSEYYFMKLFKQYTGKTLIAYINDLRIEKAKPLLLHSDSSVTEIALEVGFNNTSYFI
                     KKFQQATGMSPHKFRRNLS"
     gene            1644..3911
                     /locus_tag="ACZPD3_01690"
     CDS             1644..3911
                     /locus_tag="ACZPD3_01690"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1414754.1"
                     /translation="MENLEKLIYDLYKKNARQCTNEEIYNALLIYTKNQLFEKGYHNG
                     KKKIYYISAEFLIGKLLSNNLINLGIYDDVAAFLKENGKAIADIEEVEPEPSLGNGGL
                     GRLAACFLDSIATLGLPGEGIGLNYHLGLFKQLFENRLQKETPNPWIEEHSWLTPAGV
                     SYTVPFRGFSLKSSLYDIDVAGYNNKSIHLHLFDIDLADESMVHDGISFNKKDILHNL
                     TLFLYPDDSDDDGRKLRIFQQYFMVSNAAQFILDEATKKGCNLHDLADYAVIQINDTH
                     PSMVIPELIRLLTKRGIAFDEAAEIVSKVCAYTNHTILAEALEKWPMDYLLDVVPHLV
                     PIIEKLDEKIKAKYPQENVAIIDKNNLVHMAHMDIHYGFSINGVAALHTEILKTSELK
                     AFYDIYPEKFNNKTNGITFRRWLMHCNHPLTDYITSLIGDEFKTNATALENLLKYKDD
                     EAVLNKLLEIKTEAKKTCKEFILSNTGVEINENSIYDIQIKRLHEYKRQQLNALYIIS
                     KYLEIKGGKKPSQPVTFIFGAKAAPAYVIAKDIIHLLLTLQDLIKDDPEVAPYMKLVM
                     VENYNVSAAEKLFPACDISEQISLASKEASGTGNMKFMLNGAVTLGTMDGANVEISEL
                     VGEDNIYIFGESSEKVIEHYEKADYCSRDIYENDKRIKECVDFIVSEKMLALGHKENL
                     ERLHHELVSKDWFMTLLDFNDYVEKKDQALADYADRKTWAKKMLVNIAKAGFFSSDRT
                     IEQYNNDIWHLTPAK"
     gene            complement(4089..5300)
                     /locus_tag="ACZPD3_01695"
     CDS             complement(4089..5300)
                     /locus_tag="ACZPD3_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1414755.1"
                     /translation="MKVRMYNVGFGDCFCLRDRKKSLLVDFGTNNSRIEGRPRREIFD
                     VIISDLSTIKSKNLLLTHFHMDHLSGLLYMMKKKDISVDFGKIYLPDVFSKKEMSRTL
                     VLLLLADLLKESGLPSRQVSLFALVDALLENKQNVELLSRGKLFENKYQALWPDTDVI
                     RKETDEVYNRICEDGKFKEVMDVLLEFAEKLRLIVWSMTAEGNKPAETVEKETDLTAS
                     ELTEQPEVRQKIIRAYVYEREFRRIKALPDFKKLLAWLNENQINLRQFKHKISIVFQN
                     ARDGELNLLFTGDAQPEHLKMIAENYDGKLPLYEHYWCIKVPHHGTQGHYFDFSQYEP
                     ENMLISNGIHFANSKKESKELRTSPLYGGLFYIPDTHMYCSNCDCCDSYENGCSCKEA
                     DVISPAYYKDI"
     gene            5679..6326
                     /locus_tag="ACZPD3_01700"
     CDS             5679..6326
                     /locus_tag="ACZPD3_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR1414756.1"
                     /translation="MTKQELALEVIERLKKEYPDADCTLDYDNAWKLLVSVRLAAQCT
                     DARVNVVVQDLYAKFPTVEALANADVADIEAIVRPCGLGKSKARDISACMKILHEQYH
                     DNVPGDFDALLKLPGVGRKSANLIMGDVFGKPAIVTDTHCIRLSNRIGLVDNMKEPKK
                     VEMALWKIIPPEEGNDLCHRLVNHGRDVCTARTKPYCDRCCLNDICEKNGVDSQE"
     gene            complement(6407..6505)
                     /locus_tag="ACZPD3_01705"
                     /pseudo
     CDS             complement(6407..6505)
                     /locus_tag="ACZPD3_01705"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            6464..6868
                     /locus_tag="ACZPD3_01710"
                     /pseudo
     CDS             6464..6868
                     /locus_tag="ACZPD3_01710"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            complement(6964..7770)
                     /locus_tag="ACZPD3_01715"
     CDS             complement(6964..7770)
                     /locus_tag="ACZPD3_01715"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR1414757.1"
                     /translation="MLTIEMDNFDLGQICRSGQCFRMDQIGDDRYRVIAGDKYLELTQ
                     ERGIVNFFCPEEDFIFFWIRYFDLDCDYSEYINMINPRDKYLTAAGEMGSGIRILQQD
                     LWEMIISFLISQQNNITRIKKCIENISREFGVRKTSSTGAEYYAFPTAEALALATEEQ
                     LRECNLGYRAKYVLDTARKVCFGDISLNSLYDMTYKAARKELLGLYGVGEKVADCICL
                     FGLHQLDAFPVDTHIRQALDAHYKRGFPNRRYKGCRGVMQQYIFYYELMK"
     gene            7923..8201
                     /locus_tag="ACZPD3_01720"
     CDS             7923..8201
                     /locus_tag="ACZPD3_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR1414758.1"
                     /translation="MKFIYPAVFRKNEEGRYKAFFPDLACCEAEGDTLDDAIDNANEA
                     AYNWIYAEVMEDEMDLPAISDESDLDLLEGDIVRNIQVNIRMYDGWDE"
     gene            8368..9375
                     /locus_tag="ACZPD3_01725"
     CDS             8368..9375
                     /locus_tag="ACZPD3_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1414759.1"
                     /translation="MKARSFKKFFVSIALSAVLTLSSASSVFAATAQLAPAEQSVEQA
                     QSDDSDTPAIESSDAQNACASLTAQPGIRQTAASENSVTIQWNPVTNASKYAVNISPL
                     SSSSYRFLGYIGNTRNKAKINKLKAGTAYVIKITALNSSGIAISSRTVGCTTLYSKVK
                     IKSSYASTGRYTFNMQTVNPSNSITGYKVVYQSSAAHKLITKYFNTRYSFTIPISGNT
                     FYQVKIYPYLVLGNKRYVSSTSTDRYISNVITLQKAGNTNSSMSVKWNRTAGADNYSI
                     YIKYPGSSSFKKVKTTTSNFFTLTGMKKNTKYGIKVIANKKMKNKVWHSDSKAYNMSL
                     V"
     regulatory      9477..9585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         3024 a         2227 c         1899 g         2820 t
ORIGIN      
        1 ggatactcgg gattttcaga tcaaaactgt acagggactt caggacttcc agtcactcct
       61 ggaacaggaa aaatatgaac aggctgatac tgccatcaga aatctgaatc agacttttca
      121 gaaggaacgc tttcatcctt actgtcagaa taacctgttg caggcaatcc tggaagggaa
      181 acggcttcgt gcagaacaga aacatatcca ggtatcctac gagatccttt taccggaaaa
      241 gatttccatt aatacgactg acctgagcag tatatttttt aatcttctgg acaacgctat
      301 cgaagcttgt agcgcttccg gcaatcctga tcctgaaatc cggctgtctg caaacatttc
      361 caacggattt ctgactgtgt atatgcacaa taccaagaac ccgatgcaga cttttacaca
      421 taagactacg aaatcagaac cgggagctca tggatatggg ctttctatta ttgaagatat
      481 ctgccagaag tataatggct cttatcagtg gatcgaccat aataatactt ttgattccat
      541 tgtcttacta cagataaaaa atctctgaaa aaaggaggcg ctttccatgc cagttactcc
      601 cacgatcgaa gtagatgata ccctgaaaga acgaagttct cacggctcct ttgaacttcc
      661 catagaaact tatacagaca actgcgaaat cttccattcc ctctacaacc actggcatga
      721 cgagatggaa ctgatctata ttgaatctgg cagcggactg gtccgtctga ataaagagat
      781 cctccgcgtc aaggccggtg acctgatcat tgtgaaccgt ggggttctcc atggcataaa
      841 aacagatctg aagaatatcc tttatttccg tagtattgtt tttgacctga actttctgtc
      901 aggcccggcg ggagatttat gtcaggaacg tgtcatttcc ctgttgatgg ataatcaggc
      961 ggaatttacc catattattt ccagatctga tgacaattat gggaatatct gcctgctttt
     1021 tgacaggatc cattcctgcc ataaagaaaa aaagccgtat tatttcgtaa aattaaaggc
     1081 tctgttcttc agctttttct acgaaatgct catgggaaat tacatcactc ccgcagataa
     1141 ggaagagaac aaaagtcttg cttctattaa aaagatcctg gactacatca acctcaatta
     1201 ttcacagtca ctgaccgcgg cagagctcac tgcgctctcc aattacagcg aatactactt
     1261 tatgaaactg tttaagcaat ataccggcaa gactctgatc gcctacatca atgacctgcg
     1321 catcgagaaa gccaagcctc tgcttctgca ttccgactcc tctgtcacag agatcgcact
     1381 ggaagtaggt ttcaacaaca ccagctattt catcaagaag ttccagcagg caaccggaat
     1441 gtctcctcat aagtttcgca gaaacctgtc ataaaatcgc aattttgttg tatttttttg
     1501 aatagactgc tgatatagtt atagatgtca gcaagagaac ttctcttact gatataaccc
     1561 aagacaatac actattcaaa cctaaaaaaa taagcaatct aactcataaa aaacacatat
     1621 ttatttcaga aagggagatt acgatggaaa atttagaaaa gctcatctat gacctgtata
     1681 agaaaaatgc acgccagtgc acaaatgaag aaatttacaa cgcattatta atctatacaa
     1741 aaaatcagct ctttgaaaaa ggctatcata atggaaagaa gaaaatctat tatatttccg
     1801 cagaattcct gatcggtaaa ttattatcca acaacctgat caatctggga atctatgatg
     1861 atgttgctgc attcttaaaa gagaatggaa aagccatcgc tgacatcgaa gaagttgaac
     1921 cggaaccatc tcttggaaac ggtggtcttg gacgtctggc agcatgcttc ctggattcca
     1981 tcgcaactct cggtcttccg ggcgaaggta ttggtctgaa ttatcatctg ggtcttttca
     2041 aacagctctt tgagaacaga cttcagaagg aaactccgaa cccatggatt gaagaacaca
     2101 gctggctcac acctgcaggt gtttcctata ctgtaccgtt tcgcggattt tcactaaaat
     2161 cttccctgta cgatatcgac gttgcaggct acaataacaa atccattcat ctgcacttat
     2221 ttgacattga tctggcagat gaatctatgg ttcacgatgg aatttccttt aacaaaaagg
     2281 acatccttca caacctgaca ctgttcctgt atccggatga cagcgatgat gacggacgta
     2341 agctccgtat cttccagcag tacttcatgg tatccaatgc tgcacagttc atccttgacg
     2401 aggcaacaaa gaaaggctgc aacctgcatg accttgcaga ctacgcagtg atccagatca
     2461 acgatactca cccaagtatg gtcattccgg aactgatccg tctcctgaca aaacgtggaa
     2521 tcgcctttga tgaagctgct gaaatcgtat ccaaagtatg tgcttacaca aaccatacca
     2581 tccttgcaga agctctggaa aaatggccaa tggactacct tcttgatgta gttcctcacc
     2641 ttgtaccaat catcgaaaag ctggatgaaa agatcaaagc aaaatatcct caggaaaatg
     2701 tagccatcat cgacaagaac aacctggttc acatggcaca tatggatatc cactatggat
     2761 tctccatcaa cggtgttgct gccctgcata cagaaatcct gaaaacaagc gagctgaagg
     2821 ctttctatga tatttatccg gagaaattca acaacaaaac taacggtatc acattccgcc
     2881 gctggttaat gcactgcaat catccgctga cagactatat tacctctctg atcggtgatg
     2941 agtttaaaac aaatgcaact gctctggaaa atctcctgaa atataaggat gatgaggcag
     3001 ttctgaacaa gcttcttgag atcaaaacag aagctaagaa aacctgtaaa gaatttatcc
     3061 tctctaacac aggtgttgaa attaatgaga acagcatcta tgatatccag atcaaacgtc
     3121 tccatgagta caaacgccag cagttaaatg ctctgtacat catcagcaaa tatctggaga
     3181 ttaagggtgg caaaaaacca tctcagcctg taacctttat ctttggtgca aaagcagctc
     3241 ctgcttatgt gatcgcaaag gatatcattc atcttctgct cacactccag gatctcatca
     3301 aagatgatcc ggaagttgca ccatacatga agcttgttat ggttgaaaac tacaatgtat
     3361 ccgcagccga aaaattattc cctgcatgcg atatttctga gcagatctcc ctggcttcca
     3421 aagaagcaag tggtacaggt aacatgaagt tcatgctgaa cggtgccgtt actctgggaa
     3481 ctatggatgg cgctaatgtt gagatcagcg aactggttgg tgaagataac atctatatct
     3541 tcggtgaatc cagtgagaaa gttatcgaac attacgagaa agctgactac tgctccagag
     3601 atatttatga aaatgacaaa cgcatcaaag aatgtgttga cttcattgta agtgaaaaga
     3661 tgctggcact tggccacaaa gaaaatctcg aaagactgca ccacgaactg gtaagcaaag
     3721 actggtttat gacactgctt gacttcaacg attatgttga gaaaaaagat caggcactgg
     3781 ctgattacgc agacagaaaa acatgggcaa agaagatgct ggttaatatt gcaaaagccg
     3841 gattcttctc ttctgacaga actatcgaac agtacaacaa cgatatctgg cacctgacac
     3901 cggcaaaata atccaataaa atttgtgatc atataaattc tgtaaaaaac aaacagcagg
     3961 cattgtatat tcgatggaat atacagatgt ctgctgtttt atgtttatca gatcataaaa
     4021 atctcccact gtgttaattc aaatcagact ataactacgg caaccgaatc cgagcaaaat
     4081 ataccgcttt atatatcctt gtaataagca ggagagataa catccgcctc cttacaggaa
     4141 catccgttct cgtaactatc gcagcagtca cagtttgaac agtacatatg agtatccggg
     4201 atataaaaca agcctccata gagcggcgaa gtacggagtt cttttgactc tttcttgctg
     4261 ttggcaaaat ggattccatt agagatcagc atattttcag gttcatactg agagaaatca
     4321 aaataatgtc cctgtgttcc atgatggggc accttgatac accagtaatg ttcgtataat
     4381 ggcaattttc catcatagtt ctctgcgatc attttcagat gctcaggctg tgcatccccg
     4441 gtaaacaaaa gatttaattc tccgtctctg gcattctgga acacaatact gatcttatgc
     4501 ttgaactgcc gcagatttat ctgattctca ttcagccatg ccagcagttt cttaaaatca
     4561 ggaagtgctt tgattctgcg aaattcacgc tcatacacat atgcccggat aattttctga
     4621 cggacctccg gctgttctgt cagctcagat gctgttaaat ctgtttcttt ttccacagtt
     4681 tccgccggtt tattgccttc agcagtcata gaccatacga tcagtctcag tttctctgca
     4741 aattcaagta gaacatccat tacttcttta aatttcccgt cttcacagat tctgttatag
     4801 acttcgtccg tctccttccg gattacatct gtatccggcc agagtgcctg atatttgttt
     4861 tcaaacaatt ttcccctgct taacagttcc acgttctgtt tgttctccag aagtgcatcc
     4921 accagtgcaa acaggctgac ctgacggctt ggcagtccgg attcttttaa caaatctgcc
     4981 agaagcagca gcaccagtgt tctgctcatt tctttttttg aaaaaacatc gggaagataa
     5041 attttcccaa agtcaacaga aatatctttt ttcttcatca tataaagaag ccctgacaga
     5101 tgatccatat gaaaatgtgt cagaagcaga tttttactct tgatcgtaga caaatctgag
     5161 atgatcacat caaatatttc tcttctgggg cgcccctcta tcctgctgtt atttgttccg
     5221 aaatctacca gcagactctt ctttctgtcc cggagacaga agcagtctcc gaaacctaca
     5281 ttatacattc gtaccttcat tcttttccct cttgtttaca atggttagtt ttattttaat
     5341 atagggaaat tggaaatgca agagggtgtt gtttgtgtta ttatagtgtc gtatgattac
     5401 tggttactgt tcacatatca ctattccgcg aggtgtttta gcataaatat gccaaaatcc
     5461 cgagacatac aagccaaact cccattgccg caggcaatct ggaatgaatt ttggctgcgt
     5521 tactgagaac agagtaaaca gtaactcatc tgaaataaaa gagcgtgttt gaaaaatgtt
     5581 tttcgcaaga tgtgtgtcat gatttgtgag aatgattttt caaatacacc tgaggaaaaa
     5641 caaacataaa atatcagcag aatggagata gaacacttat gacaaaacag gaactggctc
     5701 tcgaggtcat tgaacggtta aaaaaggaat atcctgatgc agactgcact ctggattatg
     5761 ataatgcatg gaaattactg gtaagtgtgc gtctggctgc acagtgtaca gatgcaagag
     5821 taaatgtagt tgtacaggat ctgtatgcaa agtttcctac tgtagaagct cttgcaaatg
     5881 cagatgttgc agatatcgaa gcaatcgttc gtccatgtgg tctgggtaag agtaaggcaa
     5941 gagatatcag tgcctgtatg aaaatcctcc acgaacagta tcatgacaat gttcccgggg
     6001 attttgatgc tctgctaaag cttcccggag tcggaagaaa gagtgccaat ctgatcatgg
     6061 gagatgtatt cggcaaaccg gcgatcgtga cagataccca ttgcatccgt ctctccaacc
     6121 gcatcggtct ggtcgataat atgaaagaac ctaagaaagt agaaatggct ttatggaaga
     6181 tcattccgcc cgaagaaggc aatgatctct gtcaccgcct ggtcaatcat ggccgcgatg
     6241 tctgtacagc acgcactaaa ccttactgtg acaggtgctg tctgaatgat atctgtgaaa
     6301 aaaatggtgt agacagccag gaataattat tttatacaca tataaccaaa ataatccata
     6361 ccattataca cattatccca aagaaaatcc ccatctctgt gtttctttaa ccagagcgtg
     6421 tctgaaaaag gctttctgca agatgtgcgt cacaatttgt gggatatttt gcccggatga
     6481 gggcgccgta gtgggctacg gcaactgaat tcgggtgaaa tatcccgcaa agtggggcgt
     6541 gcagattgcg ggaatgattt ttcagacaca ctctagctca gcagatgagg actttcttat
     6601 tttcctaaat gtgctcctca aggcttgcga agaaaagttc atagaaatcg tcttcccctg
     6661 caagcttcgg agagttttca cctccaccgt tggtacttct tcgcatggta gcataaaatt
     6721 cttctccggc tgtgatcaga tccatctcat aaatctgata tcccagttcc cgacacttcg
     6781 cacagaaagc ggcaagcggc tgttctgcct ttctggtgtc catcagttcc tgccagatgt
     6841 tacggtcttt cagtgctttt tctcttaatt cgtctaacat tgctccgatt tccataataa
     6901 cattcctcct ctttgttttc tgaccttcat caaataacta ttccatcgtt atctgctgaa
     6961 ttgttacttc atcagttcat aataaaatat atactgctgc atcacgcccc ggcagccctt
     7021 ataacgtctg tttggaaatc cccgtttata atgggcatcc aacgcctggc ggatgtgagt
     7081 atctacagga aatgcatcca gctgatgcaa tccgaaaaga cagatacagt cagctacttt
     7141 ctcaccaact ccatatagtc ccagcagttc ttttcgtgca gctttataag tcatatcata
     7201 cagactgtta agtgaaatat ctccgaaaca gacttttctg gctgtgtcca gcacatattt
     7261 tgctctgtat cccagattac actcccgaag ctgttcctcc gttgccaaag ccagtgcctc
     7321 tgcagtcgga aacgcataat attccgcacc tgtgctgctt gtttttctta ctccaaattc
     7381 ccggctgata ttttcaatac acttctttat tcttgtaata ttattctgtt gagagatcag
     7441 gaatgaaata atcatctccc acagatcctg ctgcagaatg cggattccgc ttcccatctc
     7501 tcctgctgct gtcagatatt tatcccttgg attgatcatg tttatatatt cactgtaatc
     7561 acaatccaga tcaaaatacc ggatccagaa gaaaatgaaa tcttcttccg gacaaaagaa
     7621 attgacaatt cctctctcct gagtcagttc cagatattta tctcccgcaa tcacccggta
     7681 acggtcatca ccgatctgat ccatccgaaa gcactgcccg gatctgcaga tctgccccag
     7741 gtcaaaatta tccatttcta ttgtcagcat actatataac cccttatcct tctattacca
     7801 atgttactgt tcactccgtt ctcagtaacg ttactaataa ctcttttttt ttcttgatga
     7861 atatatgaat gtattatata ctattctaag tataaacgta aagcagaaag gggttcttac
     7921 caatgaaatt tatatatcct gcggttttcc gcaaaaatga agaaggcaga tataaagcat
     7981 tttttccgga tcttgcctgt tgtgaagctg aaggcgacac tctcgacgat gcgattgata
     8041 atgcaaacga ggctgcttat aactggattt atgcagaagt aatggaagat gaaatggatc
     8101 tccctgcaat ttccgatgaa tccgatctgg atctgctgga gggagatatt gtaagaaata
     8161 ttcaggtcaa tatccgtatg tatgacggat gggatgagta accactaccc gcaacgacaa
     8221 attacagaaa ttcagctccg ttatgttaca ataaatcaaa cattaacgga gcttttttat
     8281 tatttaaatc tgctcagaca cacttcggtg tactatctgc aaaacaattt ttttctctcg
     8341 ttttgcactt caaaaggagg ttttactatg aaagcaagaa gttttaaaaa attctttgtg
     8401 agtattgcac tcagtgctgt tctcacgtta tcgtccgctt cctctgtatt cgcagccaca
     8461 gcacagctgg cacccgctga acaatctgtg gaacaggctc aaagtgacga ttctgacacc
     8521 cctgccattg aatcatccga tgcacagaat gcctgcgctt ctcttaccgc ccagccaggg
     8581 atcagacaga cagcagcttc cgagaattct gtaactattc agtggaaccc tgttaccaat
     8641 gcttccaaat atgctgtcaa tatcagccct ttaagttcct cttcctatcg ttttctcggc
     8701 tatattggca atacccgcaa caaagctaag atcaataagc taaaagcagg aactgcttac
     8761 gttattaaaa tcacagcact gaattcatcc ggcattgcga tttcttcccg tactgtggga
     8821 tgtaccacgc tttattcaaa ggtaaagatt aaatcttcct atgcatcaac cggcagatat
     8881 accttcaata tgcagactgt aaacccttca aactccatta caggttataa ggttgtatac
     8941 cagagttctg cagcccacaa acttataact aaatatttta ataccagata ttcctttacc
     9001 attccaatat ccggcaatac attttatcag gtgaagattt acccttacct ggttctggga
     9061 aataaacggt atgtaagttc aacctctacg gacagatata tttccaatgt catcactctt
     9121 cagaaagcag gcaacacaaa cagctccatg agcgtaaaat ggaacagaac tgccggtgca
     9181 gacaattaca gtatttacat aaaatatccg ggaagcagtt ctttcaaaaa agtaaaaaca
     9241 acaacttcca atttctttac actcaccgga atgaaaaaga ataccaagta cggcattaag
     9301 gtaattgcca ataaaaaaat gaaaaacaaa gtatggcatt ctgattccaa agcctataat
     9361 atgtccttag tctgaccata gttatccata gcaattgcat aaatccggct acgttatcaa
     9421 tgacgtagcc aaaattttat attgactttc ccatatctaa gtgatacact gattataatt
     9481 gagacaaggg gtgcttgtgt aaacaggctg agagtaagct gtatcgcttt aacccattac
     9541 ctgatttgga taatgccaac gtagggaaag ttatagttct tccacactgc aatatgcttg
     9601 ttggcatatt gcagttttcc ttttactctg aacggacata tcatatatct cccccttgtt
     9661 ttaattgcgg acaaatagtc aggcaaatga tttcatacgc ctgaaaggca ataaaatatt
     9721 gccacgacag accgggggca ccactctctg tcacaccagt agtttactta aaaacttacg
     9781 aatatgcgca ggtgcaagtc cctgctgtat gcaggaggcg cagttcattt aatattgctg
     9841 tttatctatt actatgtgca gcaattaaga gcccacagtg cagggcaagg aggaaaaaaa
     9901 tgacacagta tacaacgcag atggacgcgg cacgtcaggg aatcatcaca ccacagatgg
     9961 aaatcgtagc
//
LOCUS       DBMDXG010000028         9678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1927_length_9678_cov_2.095228, whole genome shotgun sequence.
ACCESSION   DBMDXG010000028 DBMDXG010000000
VERSION     DBMDXG010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1927_length_9678_cov_2.095228"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            120..1043
                     /locus_tag="ACZPD3_01730"
     CDS             120..1043
                     /locus_tag="ACZPD3_01730"
                     /inference="COORDINATES: protein motif:HMM:NF051633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1414760.1"
                     /translation="MKSCSVSDGLAIFKALGSPVRVQILRLIQEREGLNIKQIAQNLK
                     IPVTTLSPHLNLLAEAGLIEFRDESLSHGTQKRCYFIKDWDQLLVNMALYGPDLQTYS
                     AEIGVGHFSDFSVTPTCGMATSTSFVGQLDQPRYFIHPGSRQARIVWFTTGYLEYILP
                     NFIPDHSVIEELTVSFEISSEAPRFCNVWPSDITFSLNGVILGTWTSPGDYGDRRGKY
                     NPSWWFSFLNQYGLLKKLTITPEGTFLDTEKLSDVSTRQLALTDQSIMKLRFSVLPGA
                     EHPGGCTLFGAGFGDYNQHIRITIGYHPENI"
     gene            complement(1097..2671)
                     /locus_tag="ACZPD3_01735"
     CDS             complement(1097..2671)
                     /locus_tag="ACZPD3_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR1414761.1"
                     /translation="MRKKWGMILGLTGMMLLGAQSIYAAEAADGTAETTDAANEKETA
                     GADDIESKIKKGGFTAGASVHDPSVIKDNDTYYIFGSHMESAKSTDLRNWTGFSSGVN
                     AENKLFDNLFDGEEDGDPAAFTYVGKNEEGGYSVWAPDVIYNKKMGKYVMYFCTTSSY
                     VKSNICFATADDIEGPYHYEDTFLYSGYSYHDVKESNIEELMGTDPRPYTAASYDNNN
                     WPNCIDPDVFYDKDGRMWMVYGSWSGGIFLIEIDEETGYPIYPEADEENHVDSYYGKK
                     LLGGYHNSIEGPHIMYDETNGYYYLFLSYGNLQAKGGYQMRLFRCDTVDGTYTDAAGK
                     DMYLFVEHKDHGLKMMGNYTFPSLTQTYMAPGGQTAFEDEDGKLYLVYHQRFAKTGEL
                     HEPRVHQLFRTKDGWLVAAPFATDGETLKEDGYSGDEIQGTFYLVNHGTDISDRVHKP
                     QRIQLNADGTVTGEELEGKWEAEEGTPYIDVTLGENTYTGVVLEMTDEAGNDTMCFSA
                     KGDNNETIWGVKYLLP"
     gene            complement(2716..3672)
                     /locus_tag="ACZPD3_01740"
     CDS             complement(2716..3672)
                     /locus_tag="ACZPD3_01740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR1414762.1"
                     /translation="MSEELKYNKPWILQRADPYVYRHTDGNYYFTASIPAYDRIVLRR
                     SETLAGLKDAEEVTVWEKHKEGIMSEHIWAPELHYLDGKWYIYFAGGDKDDIWAIRPY
                     VLECADTDPLTGAWTEKGKMGRADADEFSFEAFSLDATVFENKGKHYYVWAEKVGVGK
                     QISNLYIGEMETPYKLKTVQVLLTSPDYDWERVGFWVNEGPAVIHHDGRIYLTYSASE
                     TGAAYCMGMLAADEDSDLLDPKSWTKERYPVLRTDESRGIYGPGHNSFTEDEEGNPVM
                     VYHARTEAEIEGNPLYNPNRHAMLMKIRWDEKTGAPIFSYED"
     gene            3859..4782
                     /locus_tag="ACZPD3_01745"
     CDS             3859..4782
                     /locus_tag="ACZPD3_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR1414763.1"
                     /translation="MLYIKSLEQAVPVFKALGSDIRIRLIQTLLEHQEMNMNELASSL
                     GITNGALTSHVKKLEEAGILAILPEHSGHGNQKVCRINVDKILVDIASNNDSPAEDSY
                     SIDIPIGNYFNYSVYPTCGLSTTDNLIGEVDDPRYFAHPSHVDAKILWFGRGFIDYRI
                     PNMLPPGQKIDRLTLSFEISSEAPGVNNDWPSDISFFLNNTKVGSWTSPGDFGDVHGM
                     FTPDWWFPNWNQYGLLKMLVIDRHGTFIDGLKISDVNTQQLTFTSQDDMVFRFQVDEP
                     SQNIGGLTLFGKDFGNYNQDINVKVHYTPNV"
     gene            complement(4878..6806)
                     /locus_tag="ACZPD3_01750"
     CDS             complement(4878..6806)
                     /locus_tag="ACZPD3_01750"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HIR1414764.1"
                     /translation="MRDGLKERLLNKVKVTDKFWRGYQELVMDTVIPYQEKILNDEIP
                     GVEKSHALANFRIAAGLEEGEFYGMVFQDSDVAKWLESVAYALEVRPDAELEERADKV
                     IEIIEKAQQDDGYLNTFFTIKEPEHRWQNLQECHELYCAGHMMEAAAAYYEVTGKDRL
                     LHVMERMAEHIGKRFGTEEGKEPGIPGHQEIELGLLRLYEVTGKENYKDLARYFIEQR
                     GKDPDYFVKEREKRGWVHFDMDVHNREYNQAHATVYEQKEAVGHSVRAVYMYTAMAEL
                     ASLYKDEKLYQACCDLWENMTQKRMYITGGIGSTVDGEAFTIDYDLPNDTVYAETCAS
                     IGLVFFARKMLDNVMDGRYADVMERALYNGIISGMQLDGKKFFYVNPLETEPGVSGKL
                     YGYKHVLPERPGWYTCACCPPNVVRLLMSLGKYLWSETEDGVYSHIPAGTEAHFDKMD
                     VTVESNYPWDGRVTYHITGKTEEETILGIHIPSWVRPGSVQVRINGKVKDITADVEKG
                     YLILKRVWENDEVELVFPMKIRKIYANLKVREDAGCVAFMRGPMVYCFEGVDNPGLLQ
                     SYHIFEDAKMEEEVCKEGLLEGCVLLKIKARKLETVGDSLYSEVAPVRTLTTLTAVPY
                     YTWGNRGENQMRVWMRGE"
     gene            complement(6803..7075)
                     /locus_tag="ACZPD3_01755"
     CDS             complement(6803..7075)
                     /locus_tag="ACZPD3_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6171 family protein"
                     /protein_id="HIR1414765.1"
                     /translation="MNGKLRVCRKCLPGQNNEETFYEDLSRYIQRMDEDLKVDQQIYE
                     KRLNICSTCDRLMNGMCRLCGCFVELRAVQKVRKCPDLPAKWEAQK"
     gene            complement(7255..8757)
                     /locus_tag="ACZPD3_01760"
     CDS             complement(7255..8757)
                     /locus_tag="ACZPD3_01760"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIR1414766.1"
                     /translation="MTKLVINPSKKQSKINKEIYGHFSEHLGRCIYEGIYVGEDSQIP
                     NKNGMRTDVVEALKHIRIPVLRWPGGCFADEYHWKDGIGPKETRKKMINTHWGGVVED
                     NSFGTHEFFELCEQLDCEAYVNGNLGSGTVQEMSEWVEYITFEGVSPMADLRKKNGHE
                     KPWKLDFFGVGNENWGCGGNMNPDFYANEYRRYQTYVRNYHPDYPIHKVCCGANVDDY
                     EWTSEVLKTTHNHCLKELHGNMDGLSLHYYVHPEGWEIKGSATDFDDKVWYKSLNKAL
                     FMETLIERHGHIMDEYDPEKKIGMIVDEWGAWYTVEPGTNPGFLYQQNTMRDALIAGI
                     TLNIFNKHSDRVKMANLAQIVNVLQSVILTDGADMILTPTYHVFDMYKYHQDAMLVDS
                     SIETETIGVEEEWQVPNLTESVSVAEDGAVHITLTNLSLDKDYEIRTILTDYQVNEVK
                     GEIVHGEMHEMNTFETPDQVRVKEFNEVEKTAEGIRFTIPKCSVLHLEVR"
     gene            complement(9136..>9678)
                     /locus_tag="ACZPD3_01765"
     CDS             complement(9136..>9678)
                     /locus_tag="ACZPD3_01765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1414767.1"
                     /translation="GKALVKITRRMFPLSYKKEDTFIAGLSMGGYGAIRNGLKYHDTF
                     GYIAGLSSAMILEKMGIADDSSPMFFEKKSFLESVFGDLSRIKDCEINPEWIAENMKK
                     DGIPFPHMYLACGLDDPLLPPNRKFRDTMQKLGVDVTYEEGPGAHEWDFWNRYIKKVL
                     DWLPLDKDSKEGMNSGNVGL"
BASE COUNT         2441 a         2402 c         1766 g         3069 t
ORIGIN      
        1 ctatttccct atattttttt aaccaatttt atggtaaaat aactaatcaa aacgtatttt
       61 tctaaaaatt cttatttagt gttatatacc tgtaaataca gtcaaaagga ggtttttaca
      121 tgaaatcctg ttctgtttcc gatggtcttg ccatcttcaa agctctcggt tcccctgtcc
      181 gtgtacaaat tttgcgtctg atccaagaaa gagaaggtct gaatattaaa caaattgcac
      241 aaaacctgaa aattcccgtt acaaccctgt cccctcacct taatcttctg gccgaagccg
      301 ggctgataga attcagggat gaatctctct cacacggaac acagaaacgc tgctatttta
      361 taaaagactg ggatcagctt ctggtaaata tggcgcttta cggtcctgat ctgcagactt
      421 acagtgcaga aatcggcgtg ggacattttt ctgatttctc ggtaactccc acctgtggta
      481 tggcaacttc tacttctttt gtgggacagt tggatcagcc acggtatttc atacacccag
      541 gcagccgcca ggcaaggatc gtctggttca ccaccggata tctggagtat atactgccca
      601 atttcatccc tgatcacagc gtgattgaag aactgacagt ttcctttgag atcagttccg
      661 aagccccccg attctgtaat gtgtggcctt ctgatattac cttttccctg aacggtgtaa
      721 tccttggcac ctggaccagc cctggtgatt atggggaccg cagagggaaa tacaatcctt
      781 cctggtggtt ttcttttctg aatcagtatg gtttgctgaa aaaactgacg atcacccctg
      841 aaggcacttt tctggacaca gaaaagctgt ccgatgtttc caccagacag cttgcactca
      901 ctgatcagtc cattatgaaa ttgcgctttt ctgttcttcc gggcgccgaa catcccggcg
      961 ggtgtactct ttttggtgca ggtttcggag attataacca gcatatacgg attaccatcg
     1021 gttatcatcc cgaaaacatc tgatttctcc gcctgacttc agatttatat actgccccga
     1081 atactcccac agccatttat ggcaacagat atttcacacc ccatatagtt tcattattgt
     1141 cacccttagc gctgaagcac atcgtatcat tgcctgcctc atcggtcatt tccaggacaa
     1201 ctcctgtata agtattttca ccgagagtca catcaatata tggtgtgcct tcttctgctt
     1261 cccatttgcc ttccagttct tctccggtaa ctgttccgtc tgcattaagt tgaatcctct
     1321 gcggtttatg taccctgtcc gaaatatccg taccatgatt taccagataa aaagtaccct
     1381 ggatttcatc tccggaatat ccatcctctt tcaacgtctc gccgtctgtg gcaaatggcg
     1441 cagcaaccag ccatccgtct tttgtgcgga acaactgatg tacacgaggc tcatgaagtt
     1501 ctccggtttt agcaaaacgc tgatgataga ccaggtataa tttaccatct tcatcttcaa
     1561 atgcagtctg tccgcctggc gccatatagg tctgggtaag actcgggaat gtataatttc
     1621 ccatcatttt cagtccatga tctttatgct ccacaaataa atacatatcc ttccctgctg
     1681 catctgtata agttccgtct acagtgtcac agcggaagag acgcatttga taacctcctt
     1741 ttgcctgcag atttccatag gacaaaaaca gataataata tccattggtc tcatcataca
     1801 taatatgcgg gccttcgatg gagttgtgat aaccaccgag aagtttcttg ccataatagc
     1861 tgtccacatg attttcttcg tctgcttccg gataaatcgg atagccggtc tcttcatcaa
     1921 tctcaatcag aaaaataccg ccggaccagg agccataaac catccacata cgcccgtctt
     1981 tatcataaaa aacatccggg tcaatacagt ttggccagtt attattgtca taagacgctg
     2041 ctgtatatgg ccttgggtct gttcccatca gttcttcgat attactttcc ttaacatcat
     2101 gatagctata accagagtac aggaatgtgt cctcatagtg atacggacct tcaatatcat
     2161 ctgctgttgc aaagcagata ttggatttca cataagaaga tgtggtgcag aaatacatca
     2221 catattttcc catttttttg ttatagatga catccggtgc ccacacggaa taaccacctt
     2281 cctcgttctt tcctacataa gtaaacgctg ccggatcgcc gtcttcttct ccgtcaaaaa
     2341 gattgtcaaa cagcttattc tctgcattga ccccggagga aaaaccggtc cagtttctca
     2401 gatctgtgga cttcgcagat tccatatggg agccaaagat atagtaagtg tcattgtctt
     2461 tgatgacaga tggatcatgc acggaagctc cggcagtaaa gccacctttc ttaatcttgc
     2521 tctcaatatc atctgcgcct gctgtttctt tttcgtttgc cgcatctgtt gtctctgctg
     2581 tgccatcagc agcttccgca gcatatatac tctgtgctcc gagaagcatc attcctgtca
     2641 gccccaggat cattccccat ttttttctca taccagctct cctgttctcc aaatattatt
     2701 ctctctcaga ttacgttaat cttcgtaaga aaatatcggt gcgcctgttt tttcatccca
     2761 gcggattttc atcagcatgg catgacggtt tggattgtac agcggattcc cttcaatctc
     2821 tgcttctgtt ctcgcatgat aaaccatgac cggatttcct tcttcgtctt cagtgaagga
     2881 attatgcccc ggtccataga ttcctctgga ttcatctgta cgaagaaccg gataacgttc
     2941 ttttgtccag gatttcgggt caagaagatc agaatcttca tctgctgcaa gcattcccat
     3001 acagtatgca gcgcctgtct cagatgcaga ataagtgaga taaatcctgc cgtcatgatg
     3061 aataactgcc ggtccttcat tcacccagaa accaactctt tcccagtcat aatccggtga
     3121 agtgagaagt acctgaacgg ttttcagttt atacggagtt tccatttccc caatataaag
     3181 attggaaatc tgcttgccga ctcctacctt ctcagcccat acatagtaat gttttccttt
     3241 gttttcaaat actgttgcat ccagagagaa agcttcaaaa gaaaattcat ctgcatccgc
     3301 acgtcccatt ttgcctttct ctgtccaggc tcctgtaagc ggatctgtat ctgcacattc
     3361 cagcacatat gggcggattg cccagatatc atctttgtcc ccgcccgcaa aatagatgta
     3421 ccattttccg tccagataat gaagctcagg tgcccagata tgttcgctca tgattccttc
     3481 tttgtgtttc tcccagactg tcacttcttc cgcatctttc agtcctgcca gagtttcgct
     3541 tcttctcagc acaatccggt cataggccgg tatggatgct gtaaaataat agtttccgtc
     3601 tgtgtggcgg taaacatatg gatctgctct ctgcagaatc catggcttat tatacttaag
     3661 ttcttcactc attgtgtgtc cccctgttcg cttttatttt agatttatat aaattatttt
     3721 ttatatattt aaaagtgtac tgcatttttc agaaaatatc aactatattt ttttcttttc
     3781 gttgcacatt ttttgcagat tcgttataat ttaccgtaaa cgttacaaaa tcatttttaa
     3841 cagaaaggga aatccgttat gttatatatt aaaagtctgg agcaggcggt tcctgtcttc
     3901 aaagctcttg gttctgacat ccgcatacgg ctgatccaga ctctccttga gcatcaggaa
     3961 atgaatatga atgaactggc ttccagtctg gggattacca acggtgccct gaccagccat
     4021 gtaaagaaac tcgaagaagc cggaattctc gcaattcttc cagagcattc cggtcacgga
     4081 aaccagaagg tctgccgcat taatgttgac aagattttgg ttgatattgc ctctaacaat
     4141 gacagtccgg ctgaagacag ttactctatt gacattccga ttggcaacta ctttaattat
     4201 tctgtttatc cgacctgtgg gctgtctact accgataatc ttattggtga ggtagatgat
     4261 ccccgttatt ttgctcatcc gagccatgta gatgcaaaga ttttatggtt tggacgggga
     4321 tttattgatt accgcattcc aaatatgctg cctccgggac agaagattga ccggctgaca
     4381 ctctcctttg aaatcagttc agaagctccc ggagtcaata atgactggcc ttctgatatt
     4441 tcctttttcc tgaataatac aaaggtcgga agctggacaa gccccgggga tttcggtgat
     4501 gtccacggta tgttcacccc cgactggtgg ttcccgaact ggaatcagta tggtctgctg
     4561 aagatgcttg tcattgaccg tcacggtact tttatcgatg gattaaagat ctctgatgtg
     4621 aatacacagc agctcacttt tacctctcag gatgatatgg tattccgttt ccaggtggat
     4681 gaaccatctc agaatatcgg cggtctgaca ctcttcggaa aagatttcgg aaactataat
     4741 caggatatta atgtgaaagt gcattatact ccaaatgtat aattttacag agtatctgaa
     4801 aacagtaaaa gcaaggaacc gatgtgtttt gccacatagt tccttgcttt tattttatat
     4861 tccctctgat tttccggtta ttccccacgc atccagactc tcatctggtt ttctccgcgg
     4921 ttgccccatg tataatacgg cactgctgtc agtgttgtca gcgttctcac cggtgcaact
     4981 tcactgtaca gagaatctcc cactgtctcc agcttacgtg ctttgatctt aagcaggacg
     5041 cagccttcca gcagaccctc tttgcagact tcctcttcca tctttgcatc ttcaaagatg
     5101 tggtaagact gaagcagacc cggattatcc acaccctcga aacaataaac catcggcccg
     5161 cgcataaacg ctacacagcc ggcatcttct cttactttaa ggtttgcata aatcttgcgg
     5221 atcttcatcg ggaaaacaag ttctacttca tcattttccc acactctctt aaggatcaga
     5281 tatccctttt ccacatctgc cgtgatatct ttcacttttc catttatacg tacctgaaca
     5341 gaacccgggc gtacccagga tgggatatgg attcccagga tagtctcttc ctctgttttt
     5401 ccggttatat gataggtcac tctgccatcc cacggatagt tgctctctac agttacatcc
     5461 atcttatcaa agtgcgcttc tgtacctgcc gggatatgag aataaactcc atcttctgtc
     5521 tcactccaca gatatttacc aagagacatc agcagacgga ctacattggg agggcagcat
     5581 gcgcatgtat accagcccgg acgctccggc agaacatgct tatatccgta taatttcccg
     5641 ctgactccag gttctgtttc cagtggattt acatagaaaa atttctttcc gtcaagctgc
     5701 attccgctga tgatcccatt ataaagtgca cgttccatca catctgcata gcgtccatcc
     5761 attacattat caagcatctt tcttgcaaaa aataccagtc cgatagatgc acaggtttcc
     5821 gcataaacgg tgtcattcgg cagatcataa tcaatggtaa atgcttctcc gtctacggta
     5881 ctgccgattc ctccggtgat atacattctc ttctgagtca tgttctccca gagatcacag
     5941 catgcctgat agagtttttc atctttgtaa agagaagcca gttctgccat ggcagtatac
     6001 atataaacag cacggacact gtgtcctact gcttctttct gctcatagac agtagcatgt
     6061 gcctgattat attctctgtt atgcacatcc atatcaaagt gaacccagcc tcttttttcc
     6121 ctctctttga caaagtaatc cggatctttt ccacgctgtt caataaaata tctggcgagg
     6181 tctttgtaat tttcttttcc ggtcacttca tagagacgca aaagtcccag ctcgatttcc
     6241 tgatgtcccg gaatccccgg ttctttgcct tcttctgttc caaaacgttt cccgatatgt
     6301 tctgccatgc gctccatcac atgaagcaga cggtcttttc cagtcacctc ataataagcc
     6361 gccgctgctt ccatcatatg tccggcacag tataattcat ggcattcctg taaattctgc
     6421 cagcggtgtt ccggctcttt gattgtgaaa aaagtattca gataaccgtc atcctgctgc
     6481 gctttttcaa taatttcaat aaccttatct gcacgttctt ccagttcagc atccggacgg
     6541 acttccagtg catacgccac actttccagc catttggcaa catcactgtc ctggaaaacc
     6601 attccgtaaa actcaccctc ttccagtcct gctgcaatgc ggaagtttgc cagtgcatgg
     6661 cttttctcca ctcccggaat ctcatcattt aatattttct cctgataagg aattactgta
     6721 tccattacca gttcctgata tcctcgccag aatttatccg ttacttttac cttatttaat
     6781 aacctttctt ttaaaccatc tctcatttct gcgcctccca ttttgccggc agatccggac
     6841 attttcgcac cttctgtaca gcccgcagtt ccacaaagca tccgcaaagc ctgcacatcc
     6901 cattcattaa gcggtcacag gtgctgcata tgttaagcct tttttcgtaa atctgctggt
     6961 ctactttcag atcctcatcc atgcgctgaa tgtagcgtga cagatcctcg taaaaagtct
     7021 cttcattatt ctgtccgggc agacattttc tgcacacacg cagcttcccg ttcatacctt
     7081 agtctccttt ttgtttccat tataaatatt cacagaaaat acctgcctgt tttgtcagaa
     7141 gcggcatttt ctgtgaatct gtaaagttca ttcattttta tacaaaactc tttatctgtg
     7201 tcaagcgaat aaaatcatcc gcttcactac ctaattcgac taaaaatctt gtaattatct
     7261 tacttccaga tgaagaacac tgcatttagg aattgtgaat ctgattcctt ctgctgtttt
     7321 ttctacttcg ttaaattctt ttacacgtac ctggtccggt gtttcgaagg tattcatttc
     7381 gtgcatttcg ccgtgtacga tctcaccttt aacttcattt acctgatagt ctgtaaggat
     7441 agttctgatc tcataatctt tatccagtga caggtttgtc aatgtgatat gtacagctcc
     7501 atcttctgct acagatacag attctgtcag gttcggtacc tgccactctt cttccacacc
     7561 gatggtttct gtttcgatag aactgtctac aagcattgca tcctgatgat atttgtacat
     7621 atcaaataca tgataggttg gtgtcaggat catatcagca ccatctgtaa ggataacgga
     7681 ctgaagaaca tttacgatct gcgcaaggtt tgccatcttt actctgtcac tgtgtttgtt
     7741 aaagatattc agagtgatac ctgcgatcag ggcatctctc attgtgttct gctgatacag
     7801 gaagccaggg tttgtgcctg gttccactgt gtaccatgcg ccccactcgt caacgatcat
     7861 accgatcttt ttctcaggat catattcgtc catgatatgt ccgtgacgct cgatcagagt
     7921 ttccataaag agcgctttat tcaaactctt ataccatacc ttatcatcaa aatctgtagc
     7981 acttcccttg atttcccatc cttccggatg tacataataa tgaagagaaa gaccatccat
     8041 atttccgtga agttctttca ggcaatgatt atgtgtagtc tttaatactt cggaagtcca
     8101 ctcataatca tctacattgg caccacagca gactttgtgg attgggtaat ccggatgata
     8161 attgcggaca taagtctgat aacggcggta ttcatttgca tagaagtcag gattcatatt
     8221 tccgccacat ccccagttct catttccaac tccaaagaaa tccagtttcc atggtttctc
     8281 atgtccgttt ttcttacgga gatcagccat cggggaaaca ccctcgaatg tgatatattc
     8341 tacccattca gacatttcct gaactgttcc gcttcccagg ttaccattaa cataagcttc
     8401 gcagtcaagc tgctcacaga gttcaaaaaa ttcatgtgta ccaaagctgt tatcctctac
     8461 cacaccgccc cagtgtgtgt tgatcatttt tttacgtgtt tcctttggtc cgataccgtc
     8521 tttccagtgg tattcatctg cgaagcatcc acccggccag cgaagtaccg gaatcctgat
     8581 atgcttcagt gcttctacta cgtctgtacg cattccgttc ttattcggga tctgggaatc
     8641 ctcacctaca tagattcctt cgtagataca tcttcccaga tgctctgaga aatgcccata
     8701 gatttctttg ttgattttgc tctgtttctt tgatggatta attacaagct tagtcatttt
     8761 tacctccaca gtttatgatt tcaggttttt attttacctt tttctaaaat aatatacttt
     8821 attttgcatt ttcattatac acaacttctg tctgatattc aatatatttt ctgcaaaatt
     8881 gctatgataa ttttgtataa gagttactgt tcacatacca ctatcccgag acatacaagc
     8941 caaaattcca ttgccgcagg caatctggaa tgaattttgg ctaagttact gtgaacggag
     9001 tgaacagtaa cgtataagaa ggagtcctgc ccatagaact ttattctgtt acagaaacat
     9061 tacggagtaa tttcctgcaa cagaaataaa aagagccaca acagatttca tatccatctg
     9121 ttgcgactct ctcatttaca gtcctacatt cccgctgttc atcccttctt tactgtcctt
     9181 atcaagtggc agccagtcca gtactttctt aatataacgg ttccagaaat cccactcatg
     9241 tgctcccgga ccttcctcgt aagttacatc cacacccagt ttctgcatgg tatctctgaa
     9301 cttacggttc ggaggaagaa gcggatcatc cagtccacag gcaaggtaca tatgcggaaa
     9361 tgggattcca tcttttttca tattttcggc aatccattcc ggattgatct cacagtcttt
     9421 gatccgggac agatctccaa atacactctc cagaaaactt tttttctcaa agaacatagg
     9481 agaactgtca tctgctatcc ccattttttc aagtatcatg gctgaagaaa ggcctgcgat
     9541 atatccgaat gtatcatggt atttcagacc atttcggatt gcaccgtatc ctcccataga
     9601 taacccggcg ataaatgtat cttctttttt gtatgataac ggaaacattc ttcgtgtgat
     9661 ctttacaagt gcttttcc
//
LOCUS       DBMDXG010000029         9660 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1932_length_9660_cov_2.237473, whole genome shotgun sequence.
ACCESSION   DBMDXG010000029 DBMDXG010000000
VERSION     DBMDXG010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9660
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1932_length_9660_cov_2.237473"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="ACZPD3_01770"
     CDS             <1..611
                     /locus_tag="ACZPD3_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIR1414768.1"
                     /translation="VKYILAGEQACKEIEAYIAQRQQDMENTGDQSIQFELTSLNQAL
                     MMLEQRTQDLRTAENVAMQSIPMIKTMEFSNYNLVRKINSAFIVTLPVFKQALAQAIL
                     LKRQKIQAESIAELDKKTNEMLLKNAQNTVDVSKMTAKMASGSSIQIETLEKTWATIT
                     NGIEETRRIQEDARKKRQEDQVRLEAIKQDFNKQYNVPPVRK"
     gene            641..1222
                     /locus_tag="ACZPD3_01775"
     CDS             641..1222
                     /locus_tag="ACZPD3_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008750257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HIR1414769.1"
                     /translation="MPINLSKGQKVDLTKKNPGLKKIMVGLGWDVNAFDSGSDFDLDA
                     AAFMLGANGKCPTEKEFIFYGNLKHVSESVIHMGDNLTGEGEGDDEQIMIDLSKVPAN
                     IERIAFTVTIYDAEARRQNFGQVSNSYIRLVDESNEVELIHYDLGEDFSIETAVVVGE
                     LYRHNGEWKFNAIGSGFQGGLAALCGHYGIEVA"
     gene            1320..1901
                     /locus_tag="ACZPD3_01780"
     CDS             1320..1901
                     /locus_tag="ACZPD3_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HIR1414770.1"
                     /translation="MPVSLKKGQKVSLTKGNPGLKNVVVGIGWDINAFDTGGDFDLDA
                     AAFCLTDSGRVSDSNDFVFYGNLVHPSGAVQHMGDNLTGAGDGDDEQIKIDLSKIPAN
                     ITKIAFTVTIYDAEARRQNFGQVSNAFVRIFNEVTGEEILRYDLGEDFSIETAVVFGE
                     LYKNGDEWKFNAIGSGYQGGLAALCNSYGVDVE"
     gene            1929..2537
                     /locus_tag="ACZPD3_01785"
     CDS             1929..2537
                     /locus_tag="ACZPD3_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HIR1414771.1"
                     /translation="MSVSLQKGQKVNLSKEHAGLAKVIVGLGWDEAKPSGGGGLFGAL
                     FGSSSHQAIDCDASAIMLKNGKFTDKTDLVYFGNLKHKSGTVNHMGDNLTGAGEGDDE
                     QIVIDLSRVPAEYDKIVIVVNIYQAVKRKQHFGMIQNAFIRLVDARNNKEMCKYNLTE
                     NYSGMTAMIFGEIYRYNGEWKFNAVGNGTTDPGLGELCQRFV"
     gene            2642..5278
                     /locus_tag="ACZPD3_01790"
     CDS             2642..5278
                     /locus_tag="ACZPD3_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1414772.1"
                     /translation="MNEINKEERMQGLSGQQVSESKAKYGTNELAKKETESLWSMFIG
                     AFDDIWIKVLCAALALKVILAVLGIFVPALSGGNDVVEIISIVIAIALATGFSTLSEY
                     RNTSRSEALQEEYSKTYAKVVRDGKLVNILTSEIVKGDTILIQAGDKVPADGLLFEGK
                     IKVSQAALNGESRDENKAAVTNMDEAESTDYSSQGKVFMGSVVTSGEGYMIATVIGDS
                     SELGKINKALTDTSEEEERKDTSSLKLEGVAAGIGKLGVSAAAIAGILHVVLTLIRAD
                     QAITVVSVLLVIAEAVMLMASIVIMAVPEGLPMMNSLVQSMNTESMYKKNILVSHKAA
                     FSDSAYMNVLFSDKTGTITQGNLSLVEFITGNGEIVDSIQSREFIEAITLNNLAKISS
                     EGKAIGSNNMDRALLSYAIDHGYNDYDNDPEKVEEISGFDSEKKCATVKLKNGLVYWK
                     GATENIIDKVTHYMLPDGEEREFTKADKDKVEEQMHAQAKRTMKLLSVAKISDGKTVL
                     MAVLCLRDNVRTDAVETVQILNDAGIQVVMVTGDAEETAVAIAKEAGILADEKKDVVL
                     THEEMEKLSDEELKKVLPNLRVVSRAKPLDKKRLVSLSQQIDNVCGMTGDGVNDAPAL
                     KQADIGFAMGDGTAVAQEAGDVVILNNSLTSIKDCVLNSRTMAKSVGKFLIFQLTVNI
                     STLLMNIIAPILGWTEPFSIVQILWINLIMDTLAAMAFGGEPILDRYMKDQPAKRTDN
                     ILTPYIKSAIGVSAIFITLGSILILENIGGITSWVIPAGCADPELYEKTFMFAFFIYA
                     IIFNSLNTRSEKFNLFEHIGENKNFVLVMGAIFVLQTIIIEIGGKVFNTTLLEPKALL
                     VSMVLAVLIIPVDLIRKAIMSR"
     gene            5355..6140
                     /locus_tag="ACZPD3_01795"
     CDS             5355..6140
                     /locus_tag="ACZPD3_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR1414773.1"
                     /translation="MNGILCADMDNTIIYSYKRNIGENKLNVELYNGREISFISEKTH
                     DLLKKVSEKMTIIPTSTRTEEQYKRIDLDIGIVPYALVCNGGVLLVNGKRDREWYLES
                     LQMIQNSRPEMEKAQQILAGDSRRKFELRFLDELFIFTKCEKPEEVVEDLQAKLTTKL
                     VDVFHNGEKVYVVPVNLSKGMAVRRLRKRLQPAYIIAAGDSEFDVSMVEESDLGLVPA
                     GFKKIYGNGSDRFKETVMEMEAGRLFSETLLEKCLVLYFKEPD"
     gene            complement(6194..6319)
                     /locus_tag="ACZPD3_01800"
                     /pseudo
     CDS             complement(6194..6319)
                     /locus_tag="ACZPD3_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
     gene            complement(6329..>7187)
                     /locus_tag="ACZPD3_01805"
     CDS             complement(6329..>7187)
                     /locus_tag="ACZPD3_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR1414774.1"
                     /translation="KKKKRLSAQLLSDYREISSLLKQNSSLIDRLNQCSGELTLTPTQ
                     MLEKLPELMKKDFPELKDASYELRTVHPSMKKFLSPAFYLTPPVDTRTPNVIYINDSG
                     RTSSLELFGTLAHEGFPGHLYQTVSFAENNPSNIRYLVTSSGYVEGWATYVESYGYEY
                     AASLMKDPDSAKNAVRLAWLNRSMNLCIYSLIDIGIHYRGWDAARTAVFLKAFGINNA
                     STAAEIYQYIVETPGNYLKYYWGYLNFLDLKTVCQKRLGDDFDLKEFHRRILDIGPVQ
                     FPVLEKYMK"
     assembly_gap    7188..7197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7198..8084)
                     /locus_tag="ACZPD3_01810"
     CDS             complement(<7198..8084)
                     /locus_tag="ACZPD3_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR1414775.1"
                     /translation="MRIYKNIPKRILSLCLVFAIGISMGVLSDHFVSENSRFQTFTEK
                     LFRSEVCSNTLTLHYTLAHPEKKGIRKPEATLGTALSDPAKTTSLCQEYEKELKSFAY
                     SKLSEENRLTCDMLLLYFHTRASLGKNSALDEPLGPGLGVQAQLPILLAEYTFRTKED
                     ISDYLKLLSTVRPYFQSIIKLEKQKSQSGLFMSDTTLDRILKQCHSFVANPDSNYMDD
                     IFAQKLKAFSNPAFSSEDQKKLCTYHHKLILTEVIPAYQELADSLESLRGTGKSSRGL
                     AFFEGGREYYLYLLQSQTGT"
     gene            8365..9078
                     /locus_tag="ACZPD3_01815"
     CDS             8365..9078
                     /locus_tag="ACZPD3_01815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1414776.1"
                     /translation="MNNKIKTILMAAIMSSVVLTQGVCVSAAQFDDGASAFSDGTGSV
                     SALASTLAKQTEEQTQEFVAKEEEAAQIREERRVEKAEKASEKAEQEKTAALESIQQT
                     VEDTKKKIAEEAEAKRLAKRQEVVNFALQFEGNPYVYGGTSLTNGADCSGFVMSVFAN
                     FGYSLPRVAAAQCDASTKKDISQLEPGDLVFYGNGYIDHVALYIGDGKIIHASNAATG
                     IKISDYDYEKPAAVGTFME"
BASE COUNT         3021 a         1715 c         2396 g         2518 t
ORIGIN      
        1 tggtgaaata tatccttgca ggagaacagg catgtaaaga gattgaagcc tacattgccc
       61 agagacagca ggatatggag aatacgggag atcagtctat tcagtttgaa cttaccagtc
      121 tgaatcaggc actgatgatg ctggaacaga ggacacagga tctgagaaca gcagagaatg
      181 tggcaatgca gtccattccc atgataaaga ctatggagtt cagcaattac aatctggtga
      241 gaaagatcaa ttctgcgttt atcgttacgc ttcctgtatt taagcaggca cttgcgcagg
      301 caattcttct gaaaaggcag aagatacagg cagaatctat tgcagaactg gataagaaaa
      361 caaatgagat gcttcttaag aatgcccaga atacagtaga tgtatcgaag atgacagcta
      421 aaatggcatc cggaagttcc attcagattg agactcttga gaagacatgg gcaaccatta
      481 caaatggaat cgaagagaca agaaggatcc aggaagatgc cagaaagaaa cgccaggagg
      541 atcaggttcg tctggaggca atcaaacagg attttaacaa acaatacaat gtgccaccgg
      601 taagaaaata aaaacacaga aaaaagggag gatgaatgat atgcctatta atttatcaaa
      661 aggacagaag gtagatttaa caaagaagaa tccgggactt aagaagatca tggtaggact
      721 tggctgggat gtaaacgctt ttgattcagg ttcggatttt gacctggatg cagcagcatt
      781 tatgcttggt gccaacggaa aatgccctac agagaaagaa ttcattttct atggcaatct
      841 gaagcatgtc agtgaatcag tcatccacat gggagataac cttaccggtg agggtgaagg
      901 tgatgatgag cagatcatga tcgatctgtc aaaggttccg gcgaatattg agagaattgc
      961 gtttacagtt accatttatg atgcagaagc aagacgtcag aacttcggac aggtttcaaa
     1021 ctcttacatc cgtctggtag atgaatccaa tgaggtggaa ctgatccatt atgatcttgg
     1081 ggaggacttc tccattgaaa cagcagtagt tgtaggagaa ctttacagac ataacggaga
     1141 gtggaagttt aatgccatcg gaagcggatt ccaaggtgga cttgcagcgc tctgcggtca
     1201 ttatggtatt gaagtagcgt aatgtaagag acagtcggct gtttggtaaa aatactaaac
     1261 agttatcagg tatggagctg tatagcgaag cggataagac aatatgggag gaaaaatata
     1321 tgccagttag tttaaaaaaa ggacagaagg tcagtcttac aaaaggtaac ccgggactta
     1381 agaatgtagt tgtaggtatc ggatgggata tcaatgcttt tgacacaggc ggagatttcg
     1441 atctggatgc agcagcgttc tgtctgacag attccggaag ggtttctgat tcgaacgatt
     1501 ttgtattcta tggaaatctt gtacatccgt ccggagcagt ccagcatatg ggagacaacc
     1561 ttacaggtgc cggagacgga gatgatgagc agattaagat cgatctgtca aagattcctg
     1621 ccaatatcac aaagattgca tttacagtta ccatctatga tgcagaggca agacgccaga
     1681 atttcggaca ggtttctaat gcctttgtga gaatcttcaa tgaagtgaca ggagaagaaa
     1741 tcctcagata tgatcttgga gaagatttct ctattgaaac agcagttgta tttggtgaac
     1801 tgtacaagaa tggtgacgaa tggaaattta atgccattgg aagtggttat cagggtggtc
     1861 tggctgcttt gtgcaacagt tatggtgtag acgtagaata gtgccggagg gcaacaggag
     1921 ggacgaaaat gtcagtaagc ttacagaaag gacagaaggt aaatctgtca aaggagcatg
     1981 caggattagc aaaggtgatc gtgggacttg gctgggatga ggcaaagcca tccggcggtg
     2041 gtggattgtt tggtgcatta tttggttctt catcacatca ggcaatcgac tgcgacgctt
     2101 ctgcaatcat gttgaaaaat ggtaaattta cagataaaac agacctggta tatttcggca
     2161 acctgaaaca taaatccggg acagtcaatc atatgggaga caatcttaca ggagcaggcg
     2221 agggagatga tgagcagatc gttattgatc tgtcaagagt tccggctgaa tatgataaga
     2281 ttgtcattgt tgtcaatatc tatcaggcag taaagagaaa acagcatttt ggaatgatcc
     2341 agaatgcatt tatccgtctg gttgatgcca gaaacaataa agaaatgtgc aaatataatt
     2401 taactgagaa ttattcaggt atgactgcga tgatttttgg ggaaatatac aggtataacg
     2461 gtgaatggaa attcaacgcc gtaggaaatg gcacaacaga tcccggactt ggggaactct
     2521 gccagagatt tgtgtgaaaa taaaaagagg agctgttatg gttcctcttt ttatataaaa
     2581 tccggtaact gtctgtccat gattgaacag ttgcaaaacc cgaaagagga ggttgattat
     2641 tatgaatgaa ataaacaaag aggagcgaat gcagggcctg tccggacagc aggtcagcga
     2701 gagcaaggcg aagtacggca ccaatgagct ggcgaagaaa gagactgaat cactctggtc
     2761 catgttcatc ggcgcatttg atgacatctg gatcaaggta ttatgtgcgg ctctggctct
     2821 taaagttatc ctggctgtgc tgggaatctt tgtccctgca ttatccggcg gaaatgatgt
     2881 tgtggagatc atcagtattg tgatcgcaat cgcacttgct accggtttca gtaccctgtc
     2941 agagtacaga aatacttcaa gaagtgaggc gcttcaggaa gaatacagca agacctatgc
     3001 caaagttgta agagacggaa agctggtcaa tattcttacc agtgaaatcg ttaaaggtga
     3061 tacgatcctt atccaggcag gagataaggt tccggcagat ggtctgttat ttgaaggtaa
     3121 gatcaaagta tctcaggcgg cattaaacgg tgaatcaaga gatgagaaca aagcagcagt
     3181 aaccaatatg gacgaggcag aatctacgga ttactcttca cagggaaaag tctttatggg
     3241 ttcggttgtg acttcaggcg aaggttacat gatcgcaact gttatcggag acagttctga
     3301 attaggaaag ataaacaaag ctctgacaga tacctccgag gaagaagagc gaaaagatac
     3361 ttccagcctg aagctggagg gtgtggcagc aggtatcggt aaactgggtg tgtcagctgc
     3421 agcgatcgcg ggtatccttc atgttgtcct gactctgatc agggcagatc aggcaatcac
     3481 tgttgtttcc gttctccttg taatagcgga agcagttatg cttatggctt ctatcgtgat
     3541 catggcagtt ccggagggac tcccgatgat gaacagtctg gttcagtcaa tgaatacaga
     3601 gtccatgtat aaaaagaata tccttgtcag ccacaaggcg gcattttcag attctgctta
     3661 catgaacgtg ctgttcagtg ataaaaccgg aacgatcaca cagggtaacc tgtcactggt
     3721 agaatttatc acaggtaacg gtgagattgt tgattccatc cagagcaggg agtttataga
     3781 agctatcact ctgaataacc ttgcaaagat ttcctcagaa ggaaaggcaa tcggaagtaa
     3841 caacatggac agagcactgc tctcttatgc cattgatcat ggttataatg attatgacaa
     3901 tgatccggag aaggtagaag agatcagcgg atttgattcc gagaagaaat gtgccactgt
     3961 aaaactgaaa aacggacttg tttactggaa gggtgctaca gagaatatca ttgataaagt
     4021 aacacattac atgcttccgg acggggaaga aagagagttt acaaaggcag ataaagataa
     4081 agtagaagaa cagatgcatg cacaggcaaa gagaaccatg aagcttctct ccgtagcgaa
     4141 aatcagcgat ggaaagactg ttcttatggc agttctgtgt ctgcgtgata atgtcagaac
     4201 agatgcggtt gagactgttc agattctcaa tgatgcagga atccaggtag ttatggttac
     4261 cggtgatgca gaggaaactg cagtagccat tgcaaaagag gcaggtattc ttgcagatga
     4321 gaagaaggac gtggttctta cccatgaaga aatggagaaa ttatcagatg aagagctgaa
     4381 gaaagttctc ccgaacctga gagtggtaag ccgcgcgaaa ccgcttgata agaaaagact
     4441 ggtatctctt tcacagcaga ttgataatgt ctgtggtatg accggtgacg gtgtgaacga
     4501 tgctcccgca ttaaaacagg cagacattgg ttttgcaatg ggagatggta cagcagttgc
     4561 acaggaggca ggagatgttg ttatccttaa taacagtctg acttccatta aagactgtgt
     4621 gcttaacagc aggactatgg cgaaatctgt tggaaaattc ctcattttcc agcttactgt
     4681 aaatatcagt actctgttaa tgaatatcat tgcaccgatc ctgggatgga cagagccgtt
     4741 ttccattgtc cagatccttt ggatcaacct gatcatggat acattggctg ccatggcgtt
     4801 tggtggagaa cctattcttg acagatatat gaaagatcag ccggcaaaga gaacagataa
     4861 tattctgact ccgtacatca agtcagcgat cggagtaagt gcaatattta ttacccttgg
     4921 ttctatcctg atacttgaga atatcggcgg cattaccagt tgggtaatcc ctgcgggctg
     4981 tgcagatccg gagctttatg agaaaacatt tatgtttgcg ttcttcattt atgcgattat
     5041 ctttaacagt ctgaatacca gatctgagaa gtttaatctg tttgagcata tcggggagaa
     5101 taagaacttc gtactggtaa tgggagcaat ctttgttctt cagaccatta tcattgaaat
     5161 cggcggaaaa gtatttaaca caactctgct tgagccaaag gcattactgg tatccatggt
     5221 acttgcagta cttattatac cggtggatct tatcagaaaa gcaatcatga gcaggtaaca
     5281 atacgtatga aataataatt atggggctgt cgcaggtaat aacagtgtgg cagtcccatt
     5341 tttggaggaa gcgaatgaat ggcattttat gcgcggacat ggacaatacc ataatatatt
     5401 cgtataagcg caatatcggt gaaaataaat taaatgttga gttatataat ggaagagaaa
     5461 tctcatttat ttctgaaaaa acgcatgatc tgttgaaaaa agtaagtgag aagatgacta
     5521 tcataccaac atccacaagg acggaagaac agtataaacg tattgatctg gatataggaa
     5581 tcgtacccta tgcccttgtc tgtaatggag gagtactgct ggtaaatggt aagcgtgaca
     5641 gagagtggta tctggagtcg ctgcaaatga tccagaacag cagaccggaa atggaaaaag
     5701 cgcagcagat tcttgccggg gattccagac gaaagtttga gctgagattt ctagatgagc
     5761 tttttatttt tacaaaatgt gaaaagccag aggaagtggt tgaagatctg caggcaaaac
     5821 tgactacgaa gctggtggac gtatttcata atggagagaa ggtatatgtt gttcctgtaa
     5881 atctgagtaa gggaatggct gtgagaagac ttcgaaagag gttgcagcct gcatatatca
     5941 tagcagccgg agacagcgaa ttcgatgttt ccatggtgga agaaagcgac ctgggattag
     6001 ttccggccgg atttaaaaag atttacggta atggtagcga cagattcaaa gagacagtga
     6061 tggaaatgga ggcaggaaga ctgttttcgg aaacattact tgagaaatgt ttggttttat
     6121 attttaaaga gccggactga ctgcaattct ataaaatatt gcagtcagtt cggctctttg
     6181 ttatatcagg ctgttaatcc atactgacgc ggattccgct ctgacgttca gatggctgaa
     6241 tgataaccgt aacattatct cctccatgcc actcaaactg ataagactct ccggaagtct
     6301 ggccgatgaa agttttccag taattcgttt atttcatgta tttttccaga accggaaact
     6361 gtacaggtcc gatgtccaga atccggcggt gaaattcttt caggtcgaaa tcatctccaa
     6421 gtcttttctg acagactgtt ttcaagtcaa ggaaattcag atatccccaa tagtatttca
     6481 gatagttacc gggagtttcc acaatatact gatagatttc ggcagcagta gaagcattgt
     6541 tgatgccaaa tgctttcaga aagacagcag tgcgggctgc atcccagccc ctgtaatgaa
     6601 tgccgatatc gatcagggag taaatgcaca gattcatact cctgtttaac caggcaagcc
     6661 ggacagcatt tttggcagaa tccggatcct tcatgaggga ggcggcatat tcatagccgt
     6721 aggattccac gtaggttgcc cagccttcca cgtaaccgga ggaggtgacc agatatctga
     6781 tgttagaggg attgttttcg gcaaaagaaa ctgtctggta aagatggccg ggaaatcctt
     6841 catgtgccag tgtgccgaaa agttccagag aagaggtacg tccggaatcg ttgatataga
     6901 ttacgttggg ggttcgggta tccacaggcg gcgtgaggta aaaagcagga cttaagaatt
     6961 ttttcatgga aggatgaacg gtgcggagtt cgtaagaggc atctttcagt tccgggaaat
     7021 cttttttcat caattccgga agcttctcaa gcatctgagt cggtgtcagc gtaagttcac
     7081 cggaacattg attcagcctg tcgatcaggg aggaattctg tttcagcagg gaagatatct
     7141 cccgatagtc ggacaaaagc tgagctgaca gtcttttttt tttttttnnn nnnnnnngtt
     7201 cctgtctggc tttgcagcag gtagaggtaa tattcacgcc caccctcaaa aaatgccagg
     7261 ccccggctgc ttttgcctgt accgcgaaga ctttccagac tgtctgccag ttcctgataa
     7321 gcagggatca cttcggttaa gatcagttta tgatgatatg tacagagttt tttctgatct
     7381 tcgctgctaa atgcagggtt ggagaaagct ttcagtttct gcgcgaaaat atcatccatg
     7441 tagttggaat caggattcgc gacaaaagaa tgacattgtt tcaggatacg gtccagagtt
     7501 gtgtcgctca tgaaaagccc tgactgggat ttctgctttt ccagttttat aatactttga
     7561 aaatatggcc tgacggtact cagaagtttc agataatcag agatatcttc ttttgtccgg
     7621 aaagtatatt ccgccagaag aatgggaagt tgtgcctgca cacccaggcc tggtcccagc
     7681 ggctcatcca gggcgctgtt ttttccaagg gaggctctgg tgtgaaaata gagaagaagc
     7741 atatcacagg taagccggtt ttcttcggag agcttggaat aggcaaagga tttcagctct
     7801 ttctcatatt cctgacagag actggtggtc ttagccgggt cagaaagtgc agttccaaga
     7861 gttgcctcgg gctttctgat accttttttt tcagggtgtg caagagtgta gtgaagggtg
     7921 agggtattgc tgcatacttc ggaccggaat aatttttccg taaaggtctg aaaacgggag
     7981 ttttcagaaa caaagtggtc ggagagaaca cccatgctga tgccaatggc aaagaccaga
     8041 cacaggctga ggattctttt agggatattt ttataaatac gcatatccac ctcggaatct
     8101 gctttttact atcttattga aggaaggcat gaagtatgct ttcggtaaca tcattactgt
     8161 tcacacacca ttatcccgcg aggtgttttg gcataaatat gccaaaatcc tgagacatac
     8221 aagccaaaat tcattccaaa tgctcgtcac acttttttca tttgacaaaa ttgggatgtt
     8281 atgttaacat acacaaatgc gtaacaaaac gtaataaaaa tgtaataatt ttacaacgat
     8341 gtataacagg agttgattag taaaatgaat aataaaataa agacaatttt gatggcagca
     8401 attatgagct ctgtggttct gacacagggg gtatgtgtaa gcgcagcaca gtttgatgat
     8461 ggcgcatcag cgttttctga tggaacagga agtgtaagtg ctctggcaag tacactggcg
     8521 aagcagacag aggaacagac acaggagttt gttgcgaaag aagaggaagc agctcagatt
     8581 cgtgaagaac gaagagtaga gaaagcggaa aaggcttctg agaaggcaga gcaggaaaaa
     8641 acagccgcgc tggagagtat tcagcagaca gttgaggata caaagaaaaa aattgccgaa
     8701 gaagcagaag cgaaacgtct ggcaaagaga caggaagtgg taaactttgc gctgcagttt
     8761 gaaggaaatc cttatgttta tggcggaaca agtcttacaa acggtgcaga ctgctcagga
     8821 tttgtaatgt ctgtatttgc caatttcggt tacagtcttc cacgtgtggc tgcagcgcag
     8881 tgtgatgcct ccacaaagaa agatatcagc cagcttgagc cgggagatct ggtattctac
     8941 ggaaacggat atattgatca tgtggcactt tatatcggag atggaaagat tattcatgcc
     9001 agtaatgctg ctacaggaat caagatttcc gattatgatt atgagaagcc ggcagctgta
     9061 ggtacgttca tggaatagct gtaacgccta aaattatatg attcgacaaa ttgcacaaag
     9121 aatgtcgaga ggtgggaaaa gtggataaag tcatacacac tctatccaca tacccggtta
     9181 ttgaaaaatc ccgtaaatta aagttataca caaagttatc cacattatcc acattttatg
     9241 tatgtggaat actcgattta cataaggaaa aaagaaacag atgttttgtg taaaatgata
     9301 aatcgacatt tttggacgaa aaaatccaca aatctattga cttttttata ttctaaaaaa
     9361 catataatat tgtacaaaaa atacaaaata taacaatata tataaaaata atcagaatat
     9421 taaaaaagat attctactaa aaaatacaac aacaatgaaa aaaagaatac atttgacaga
     9481 aacaggaaat ttccacatag gagagacaga tgtggatgag aaatgcacaa aatgaattcg
     9541 ggtggggata acttttcaca gatattatcc acataacatg aagtcctgtg tcagatatct
     9601 ttgccgtgcg tcagcacgcc tgcagatatc ttccttgtct ttcgcactct gctgaactgt
//
LOCUS       DBMDXG010000030         9509 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1966_length_9509_cov_1.735969, whole genome shotgun sequence.
ACCESSION   DBMDXG010000030 DBMDXG010000000
VERSION     DBMDXG010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1966_length_9509_cov_1.735969"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="ACZPD3_01820"
     CDS             complement(<1..93)
                     /locus_tag="ACZPD3_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR1414777.1"
                     /translation="MENFVITIARQYGSGGRTVGKMLAEKLGVSF"
     gene            complement(108..818)
                     /locus_tag="ACZPD3_01825"
     CDS             complement(108..818)
                     /locus_tag="ACZPD3_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414778.1"
                     /translation="MALLEVQDIQVYYGMIQALKGVSFSVNEGEVIALIGANGAGKTT
                     TLHTVTGLLRAKSGHIIYDGQDITKVPPHKIVTMGMAHVPEGRRVFANMTVLQNLKMG
                     AFTRSDKNEIDATIEKVYKRFPRLKERQNQTAGTLSGGEQQMLAMGRALMSQPKIILM
                     DEPSMGLSPIFVNEIFDIIKEVSESGTTVLLVEQNAKKALSIADRAYVLETGRITLEG
                     KASDLLHDESVQKAYLGE"
     gene            complement(831..1589)
                     /locus_tag="ACZPD3_01830"
     CDS             complement(831..1589)
                     /locus_tag="ACZPD3_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414779.1"
                     /translation="MALLEVNHLNISFGGLHAVDDFHVSIEKGQLYGLIGPNGAGKTT
                     IFNLLTGVYKPDEGIINLDGQDITGKSTIDINKAGIARTFQNIRLFHQMSVLDNVKAG
                     LHNHDKYNLFTSIVHTPKYFKVEKTMNEEAMKLLKVFDLDKEADILASNLPYGKQRKL
                     EIARALATKPKLLLLDEPAAGMNPNETEELMDTIRFVRDNFDMTILLIEHDMKLVSGI
                     CEELTVLNFGHVLRQGKTSEVLHDPEVIKAYLGE"
     gene            complement(1593..2648)
                     /locus_tag="ACZPD3_01835"
     CDS             complement(1593..2648)
                     /locus_tag="ACZPD3_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1414780.1"
                     /translation="MEKKKLNKTTKSNLITYGMVLAAYLIVQGMIAGNLLSNLMQGLL
                     VPLCVYIILALSLNLTVGILGELSLGHAGFMCIGAFTSAAFTKATMDTFTNAGLRFFV
                     ALIIGAVCAGIFGFLIGIPVLRLKGDYLAIVTLAFGEIIKNIINVLYVGIDSNGIHFS
                     MKDQMSLGMEPGGKMIISGAMGITGTPRQSTFTIGVILILVTLFVVLNLINSRDGRAI
                     MAIRDNRIAAESIGIDITKYKLKAFAISAALAGIGGVLYAHNLATLTALPKNFGYNMS
                     IMFLVFVVLGGIGNMCGSIIAAVILNLLPELLRGLSDYRMLFYAIVLIVMMLINWAPK
                     AIEMRERLFSKFKKKEA"
     gene            complement(2659..3546)
                     /locus_tag="ACZPD3_01840"
     CDS             complement(2659..3546)
                     /locus_tag="ACZPD3_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR1414781.1"
                     /translation="MSSFISYLISGISLGSVYAIIALGYTMVYGIAKMLNFAHGDVIM
                     VGSYVVYVAVSGMGLNPILAILLSIIICTLMGVVIEGVAYRPLRNAASPLAVLITAIG
                     VSYLLQNVALLIWGADTKSFSNVISLPSLKLAGGSIVITGVTIVTIIGGILIMAGLML
                     FISKTKTGQAMLAVSEDKGAAQLMGINVNKTISITFAIGSGLAAIAGVLLCSAYPSLT
                     PYTGAMPGIKAFVAAVFGGIGSIPGAFIGGVVLGILEIFGKAYISSQMADAIVFGVLI
                     VVLVVKPTGILGKNIQEKV"
     gene            complement(3795..4949)
                     /locus_tag="ACZPD3_01845"
     CDS             complement(3795..4949)
                     /locus_tag="ACZPD3_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1414782.1"
                     /translation="MKNLKKIISVTAAAAMVMSTVAPVSVFADDATFKIGGIGPVTGA
                     AAIYGQAVKNATELAINEVNEDGGINGYQVEFKFEDDENDAEKTLNAYNALKDWGMNV
                     LVGTVTSTPCVAVANETAADNMFQLTPSGSATECVANPNVFRVCFSDPNQGTAAAQYI
                     GEHKLATKVAIIYDSSDVYSSGITQNFTAEAANQGIEIVSSEAFTADNNKDFSVQLQK
                     AKDGGAELVFLPIYYTEASLILAQADSMGFETKFFGCDGMDGILGVENFDTSLAEGLM
                     LLTPFAADSTDEKTQNFVKAYKDAYKDTPNQFAADAYDAVYAVKAAAEKEDVKADMDV
                     ADICAALEKGMTEITLEGVTGDEITWTEDGEPNKAPKAVEIKDGAYAAME"
     gene            complement(5379..5735)
                     /gene="rplT"
                     /locus_tag="ACZPD3_01850"
     CDS             complement(5379..5735)
                     /gene="rplT"
                     /locus_tag="ACZPD3_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR1414783.1"
                     /translation="MARIKGGLNAKKKHNRTLKLAKGYRGARSKQYRVAKQSVMRALA
                     SSYAGRKQKKRQFRQLWIARINAAARMNGLSYSKLMHGLKVANIDINRKMLAELAVND
                     AEGFTALAEIAKKAIA"
     gene            complement(5783..5980)
                     /gene="rpmI"
                     /locus_tag="ACZPD3_01855"
     CDS             complement(5783..5980)
                     /gene="rpmI"
                     /locus_tag="ACZPD3_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1414784.1"
                     /translation="MPKIKTCRAAAKRFKKTGTGKLVRNKAYKSHILTKKSQKRKRNL
                     RKSTVVDATNVKSMKKALPYL"
     gene            complement(6016..6510)
                     /gene="infC"
                     /locus_tag="ACZPD3_01860"
     CDS             complement(6016..6510)
                     /gene="infC"
                     /locus_tag="ACZPD3_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1414785.1"
                     /translation="MINEQIRDKEVRLIGSDGEQLGIMSAKEAMFKAQEAGLDLVKIA
                     PQAKPPVCKIIDYGKYRYELARKEKEAKKKQKTIDVKEVRLSPNIDTNDLNTKVNAAR
                     KFLSKGDRVKVTLRFRGREMAHMASSRHVLDDFAKQLADVATVEKAPKVEGRSMTMFL
                     TEKR"
     gene            7071..7400
                     /locus_tag="ACZPD3_01865"
     CDS             7071..7400
                     /locus_tag="ACZPD3_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR1414786.1"
                     /translation="MTILKCSAMTCVYNKEQLCSKGDIDVTGENATSANETSCGSFRE
                     RTGSSMKDSYTDDCGCDKIQIDCKAHNCTYNDNCKCTASSIQVDGSNAHASSDTRCDT
                     FQCHCGA"
     gene            complement(7547..8725)
                     /gene="ftsZ"
                     /locus_tag="ACZPD3_01870"
     CDS             complement(7547..8725)
                     /gene="ftsZ"
                     /locus_tag="ACZPD3_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1414787.1"
                     /translation="MSNEAESSAKIIVIGVGGAGNNAVNRMVEEAIGGVEFVGVNTDK
                     QALTLCKAPTVLQIGEKITKGLGAGAQPEVGQKAAEESIEEVKQLMEGADMVFVTCGM
                     GGGTGTGAAPVIAAAAKEMGILTVGVVTKPFRFEARTRMNNALAGIENLKKAVDTLIV
                     IPNDKLLEVVDRRTTMPEALKKADEVLQQAVQGITDLINLPALINLDFADVQTVMTDK
                     GIAHIGIGEARGDDKAMEAVQQAVSSPLLETTIKGATHVIINISGDISLMDANDAASY
                     VQELTGEEANIIFGAMYDDTVADYCRITVIATGLNDDNLQQTPFGKRSTASSFGARRP
                     SSTATTGTATAGTATGSGMNMPSFSLPTMNATPFGASKTPSSTVQKKDIQIPDFLRNR
                     "
     gene            complement(8943..>9509)
                     /locus_tag="ACZPD3_01875"
     CDS             complement(8943..>9509)
                     /locus_tag="ACZPD3_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR1414788.1"
                     /translation="QLGKDADLEEKMNRVIAILPKIQGKKGILHMESVATESNTFEEE
                     LEQEEVTAENWNGGYDEDGNYTGDGEYDEDGNYVGAKPKTALEEAIENWNGGYDEEGD
                     YTGAGEYDESGNYVGPKPTQESLDAASDESGDGSGDESSADDQSSETSEDGESDSSYD
                     DGNSDDSDGYGDYTEDNSDYQGDDSEGY"
BASE COUNT         2503 a         2279 c         1729 g         2998 t
ORIGIN      
        1 gaaagaaact cccagtttct ctgccagcat cttaccgacg gtacgtccgc cgcttccata
       61 ctgtctcgca atggtaatta caaaattctc cataaaatcc ctcctgttta ctctccaaga
      121 taagctttct gaacagattc atcatgaaga agatcacttg ccttaccttc cagtgtaatc
      181 cttccggtct caaggacata agctctgtca gcaatagaaa gagccttctt cgcattctgc
      241 tcaaccagaa gaaccgttgt tccactctca cttacctctt taatgatatc aaagatctca
      301 tttacaaaga tcggtgaaag gcccatggac ggctcgtcca tcagaatgat cttcggctgg
      361 ctcatcagcg cacgtcccat ggcgagcatc tgctgctcac caccacttaa ggtacctgca
      421 gtctgattct gacgttcctt cagacgcgga aaacgtttat atactttctc aatggtcgca
      481 tcaatctcat ttttatcaga tcttgtaaaa gcacccatct tcagattctg aagaaccgtc
      541 atatttgcaa acacacgtct tccttccgga acatgtgcca tacccattgt tacgatcttg
      601 tgaggcggaa cctttgtgat atcctgtccg tcataaataa tatgtcctga ttttgcacga
      661 agaagccctg tcactgtatg cagagtagta gttttaccgg caccattggc accgatcaga
      721 gcaataactt caccttcatt tacagagaaa gaaacaccct tcaatgcctg aatcattcca
      781 taataaacct gtatgtcctg tacttccagt aatgccatct gtctctcctc ctattctcca
      841 agatatgcct tgataacttc cggatcatgg agcacctctg aagtcttgcc ctgtctcaga
      901 acatgaccga agttcagtac agtcagctcc tcacagatac ctgaaaccag cttcatatca
      961 tgctcgatca gaagaatggt catatcaaag ttatcacgaa caaaacgaat ggtatccatc
     1021 agttcctcag tttcattggg gttcatacct gcagccggct catccagaag aagaagcttc
     1081 ggctttgttg ccagtgctct ggcaatctcc agctttctct gtttaccata aggaaggttg
     1141 gatgcaagga tatctgcctc tttgtccaga tcaaacactt taagaagctt cattgcttcc
     1201 tcattcatgg ttttctcaac cttaaagtat ttcggagtat gtacaatact tgtaaaaagg
     1261 ttgtatttat catgattgtg aagtcctgcc tttacattgt ccagcacact catctgatgg
     1321 aacagacgga tattctggaa tgttctggcg attcctgcct tgttgatatc aatggtactc
     1381 tttccggtga tatcctgtcc gtccagattg atgattccct cgtccggctt atacacacct
     1441 gtaagaaggt taaagattgt agtcttaccg gcaccattcg gaccgatcag accataaagc
     1501 tgtcctttct cgatggagac atggaaatca tccactgcat gaagaccgcc gaatgaaata
     1561 tttaaatgat ttacttccag caatgccata gcttatgcct cctttttctt aaatttactg
     1621 aaaagacgct ctctcatctc gattgcctta ggtgcccagt tgatcagcat cattacgatc
     1681 agcacgattg catagaacag catacggtaa tcactgagtc cacgcagaag ctcaggaaga
     1741 aggttcagaa tcactgccgc aataatagaa ccacacatat ttccaattcc accgagaact
     1801 acgaatacca ggaacatgat agacatgtta taaccgaagt tcttcggaag tgctgttaaa
     1861 gttgcaagat tatgagcata aagcacaccg ccgataccgg ctaatgctgc tgagattgca
     1921 aatgctttca gtttatactt tgtgatatca ataccaattg attccgctgc aatacggtta
     1981 tcacggattg ccatgatcgc acgtccgtct ctggaattga tcaggttcag aactacgaat
     2041 aaagtaacca ggatcagaat cacacctata gtaaaggttg actgtctcgg tgtacctgtg
     2101 atacccatgg caccactgat gatcattttt ccaccaggct ccattccaag agacatctgg
     2161 tctttcatgg aaaaatggat tccgttgctg tcaattccta cataaagcac attgatgata
     2221 ttcttaatga tctcaccaaa tgccagtgtt acgatagcaa ggtagtcacc tttcagacga
     2281 agtacaggaa taccgatcag gaatccaaag atacctgcac atacagctcc gataatcaga
     2341 gcaacgaaga aacgaagtcc tgcatttgta aatgtatcca tagttgcctt tgtaaatgct
     2401 gcactggtga aagcaccgat acacataaat ccggcatgtc caagacttaa ctcacccaga
     2461 ataccaaccg ttaggttcag ggaaagagcc aggataatat atacacaaag aggcaccaga
     2521 agaccctgca tcaggttgga aagcagattc cctgcgatca ttccctgtac gatcagatat
     2581 gctgcaagaa ccattccgta tgtaatgaga ttgctttttg ttgttttgtt taatttcttc
     2641 ttttccatgt ctgccacctc acactttctc ctgaatgttc ttaccaagaa taccggtagg
     2701 tttcacaacc agaactacga tcagaacacc gaatacaatg gcatcagcca tctgtgaaga
     2761 gatataagct ttaccaaaaa tctccaggat acccagaaca acacctccga taaaggctcc
     2821 cgggatagat ccgataccgc caaatacagc tgcaacgaat gctttgatac caggcatagc
     2881 acctgtatat ggtgtcaaag acgggtatgc agagcagaga agcacacctg cgattgctgc
     2941 aagtccggaa ccgattgcaa aagtaatgga gattgttttg tttacgttaa tacccattaa
     3001 ctgggcagcg cctttatcct cagatacggc aagcattgcc tgccctgttt ttgtcttgct
     3061 gataaagagc atcagacctg ccataatcag gataccaccg ataatggtta cgatggttac
     3121 accagtgatc acgatagaac cacctgcaag tttcagggat ggaagactga tcacgttact
     3181 gaatgatttg gtatctgctc cccagatcag aagggccacg ttctgaagca ggtagctgac
     3241 accaattgct gtgatcagaa cagccagagg tgaagctgca ttacgcaatg gtcgatacgc
     3301 aacgccttcg atcacaacgc ccatgagtgt acagatgata atggacagca ggatggctaa
     3361 aatcggattt aatcccatgc cgctgactgc gacataaact acataagagc cgaccatgat
     3421 aacatctcca tgagcgaagt tcagcatttt agcgattccg tataccatgg tataaccaag
     3481 agcgatgatc gcatatacac ttccgaggct gataccactg ataagatatg atataaaact
     3541 actcatagaa gtctttcctt tcttatattt aaataatttt attgtgtgta aaactggcag
     3601 atttgccttt agcgaaatct gtcttgttta ttttgccaaa agtaaatctg tcattaacgc
     3661 attaatgtaa taacgtatta ggcaacaggg ctgtctgcat aattcagaca gccccgttgg
     3721 catgcaacca ttgtccactc caggaacaat cgcattttat ttcttatgaa atcttttcag
     3781 ttatttcaat ttcattattc cattgctgcg taagcaccat ctttgatttc tacagctttc
     3841 ggagctttgt tcggctcacc gtcttctgtc catgtgatct cgtcacctgt aacaccttca
     3901 agtgtgatct cggtcatgcc tttttcaagt gctgcacaga tatctgcaac atccatgtct
     3961 gcttttacat cttctttttc tgctgctgct ttgactgcgt atactgcatc ataagcatct
     4021 gctgcgaact ggtttggtgt gtctttgtaa gcatctttgt atgcttttac aaagttctgg
     4081 gttttctcat ctgtagaatc tgctgcaaat ggtgtaagaa gcatcagccc ctctgcaaga
     4141 gatgtatcaa agttttctac accaaggata ccgtccatac cgtcgcatcc gaagaattta
     4201 gtttcaaatc ccatgctgtc tgcctgtgca aggatcaggg aagcttctgt ataataaatc
     4261 ggaaggaata ctaattcagc accaccgtct tttgctttct gaagctgtac ggagaagtct
     4321 ttattgttat cagcagtaaa tgcttcggaa gatacgattt caataccctg attagctgct
     4381 tctgctgtga agttctgtgt gatacctgat gaatatacat cagagctatc gtagataata
     4441 gctacttttg tagcaagttt atgctctcca atgtactgag ctgctgcagt accctggttc
     4501 gggtcagaga agcatacacg gaatacgttc ggattagcaa cacactctgt tgcagatccg
     4561 gatggagtaa gctggaacat attgtctgct gctgtttcat ttgctacagc tacacaaggt
     4621 gtagaagtaa ctgtaccaac caggacgttc attccccagt cttttaatgc gttgtaagcg
     4681 ttcagggttt tctcagcatc gttttcatca tcttcaaatt tgaactctac ctgatatccg
     4741 ttgattccgc cgtcttcgtt tacttcattg attgcaagct ctgttgcatt ctttacagcc
     4801 tgtccataga ttgctgctgc tccggttaca ggtccgattc caccgatctt aaatgtagca
     4861 tcgtctgcaa atacagatac aggtgcaact gttgacatta ccattgctgc tgccgcagta
     4921 acactgatga tttttttcag gtttttcata ataaatcctc cttatatttt gtcagtcctt
     4981 tcgtccctct tacggctcat aagattagct tccgttacca ttcattccac ccggtatatc
     5041 attttcacca gattccgcaa gttcatttat tatgtagagg aactgaaaag taacaaaaag
     5101 ccaactgggt tcccagccgg ctttggcttc cactgacact ttaatatgtt gctgttttga
     5161 attatatcac catatatttg accatgcaag aaaaacttta tggaaattta agatttttcc
     5221 gtctttcatg gacacttgac cgtaccgcgc acgcaatttt atcccattca tcgaaaaatg
     5281 cattaaaaaa ggtacacata gaaaccgtaa caatttctat gtgtacctca aaattctgat
     5341 ttaatttatc aaatcttatg tctctctaaa accgacaatt atgcaattgc ttttttagcg
     5401 atctcagcaa gtgctgtaaa tccttctgca tcgtttactg caagttcagc aagcatcttt
     5461 ctgttgatgt cgatgtttgc aactttcagt ccgtgcatca gtttgctgta ggataatccg
     5521 ttcattcttg ctgctgcgtt gatacgagcg atccagagct gtctgaactg acgttttttc
     5581 tgctttcttc ctgcatagga gctagcaagt gctctcatta cagactgttt agcaactcta
     5641 tactgtttgg aacgggctcc tctgtagcct tttgccagtt ttaatgtacg attatgtttt
     5701 ttctttgcgt ttaatccgcc tttaattctt gccatttctt aaatttcctc ctgtttgtct
     5761 atcttcaatg aataaatgct tcttataaat atggtaatgc tttcttcatg ctcttaacat
     5821 tagtagcatc aacaacagta gattttctca gatttctttt tcttttctga gatttctttg
     5881 ttaagatatg gctcttataa gctttatttc ttactaattt tcctgtacct gtttttttga
     5941 aacgctttgc tgctgctctg caagttttaa tttttggcat tgttaattcc tccttaaact
     6001 gtatctctat tatatttaac gtttttccgt taaaaacatg gtcatgcttc tgccttcaac
     6061 cttcggtgcc ttctccactg ttgccacatc tgcaagctgc tttgcaaaat catcaagcac
     6121 atgtctgctg cttgccatat gtgccatttc tcttccacgg aaacgtaatg ttacctttac
     6181 tctgtcaccc ttggagagga actttctggc ggcgttgact ttcgtattca ggtcattggt
     6241 atcaatgttt ggagaaagtc gcacttcttt gacatcgatg gtcttctgct tcttcttggc
     6301 ttctttttct tttcttgcaa gctcataacg gtatttacca taatcgatga tcttacatac
     6361 cggcggtttc gcctgcgggg caatttttac caggtcaagc cctgcctcct gagctttgaa
     6421 cattgcttct ttcgcggaca tgattccaag ctgttctccg tccgaaccga ttaagcgtac
     6481 ctctttgtct ctgatctgtt cgttaatcat taaattgcta attgtagtgc acctccatac
     6541 ctgttagata gattctaatg acttctcgtc gccctcattt acataaaaaa agcgtggaca
     6601 aaatccacac tccgctatca cactgctgat atcttttctt cttaatctgc ggaacaatct
     6661 ccttattcct gccaacctta aatatcagcc ttctctgtgc aatataaacc caagccgcta
     6721 aactaaatgc gctgaggtga gagtggaact ctgctttgtt ttcttacgta cttgtataag
     6781 ataccatgtt cagtctgcta tgtcaagtgc tttttgaaat tttctttatt ttatttcttt
     6841 attattttca gaccttttct ttgaaatttt atctgaattt ctatatggat ttttctctga
     6901 actttcccga agtaatccga tataaattat cgcagtcctc gtaaaatcat gtatattttc
     6961 tctcaaactg cacatcctat ctccgaaact tattcaatgt aactgttcag tactcttgca
     7021 ctacagcagt attgaatagt tatctcagat tttttcagga gggtaactct atgacaattc
     7081 ttaaatgttc agcaatgacc tgtgtttaca acaaagaaca gctctgttca aagggtgata
     7141 ttgacgtgac aggcgaaaat gctacttctg caaatgagac aagctgcggc agtttccgag
     7201 agagaaccgg cagttccatg aaagacagct atacagatga ctgtggctgc gacaaaatcc
     7261 agatcgactg caaagcccat aactgtacct ataatgataa ctgcaaatgt acagcatcct
     7321 ccattcaggt agacggctct aatgcacatg catcttccga tacaagatgt gacaccttcc
     7381 agtgccattg tggcgcataa tatcaacacc atggcaacag gattttcacc cgtatatcag
     7441 ggataatgaa cttccctgat ctggtcaaaa aaagagacat gtccatctat atggaatatg
     7501 tctcttttgt ctgtctgtaa attatttcac tgcaaaacag ataatcctat ctgtttctta
     7561 agaaatccgg aatctggata tccttcttct gaacagtgct tgacggagtc ttagatgctc
     7621 caaatggtgt ggcattcata gttggaaggc tgaagctagg catgttcata ccgcttcctg
     7681 ttgcagttcc ggctgttgct gtacctgtcg ttgctgtgga agaaggtctt cttgcaccaa
     7741 aggaagaagc tgtgcttctt tttccgaaag gggtctgctg aaggttatca tcattcagac
     7801 ctgttgcaat cactgtgatt ctgcagtagt cagcaactgt atcatcatac atggcaccaa
     7861 agatgatgtt tgcttcctca cctgtaagct cctgtacata gcttgcagca tcattggcat
     7921 ccataagaga aatatctccg gaaatattaa tgataacatg tgtagcacct ttgatggttg
     7981 tctcaagaag tggagaagat acggcctgct gtacagcttc catagctttg tcgtctcctc
     8041 ttgcttcacc gataccgata tgggcaattc ccttgtctgt cattacagtc tgaacgtctg
     8101 caaagtcaag gttaatcaga gccggcagat taatcaggtc tgtaatgccc tgtactgcct
     8161 gctgcagaac ttcatcagcc ttcttcagag cttccggcat ggttgttctg cgatctacaa
     8221 cttccagtaa tttatcattc gggataacga ttaatgtatc cactgctttc ttcagattct
     8281 caatgcctgc aagtgcatta ttcatacgag ttcttgcttc aaaacggaaa ggttttgtaa
     8341 caacgccaac tgtaaggata cccatttctt tcgctgctgc tgcgataaca ggtgcagctc
     8401 cggttccggt tccgcctccc ataccacagg taacgaatac catatccgca ccttccatta
     8461 actgttttac ttcttcaatg ctctcctctg ctgctttctg tccaacctca ggctgggctc
     8521 ctgcaccaag acctttggta atcttctcgc caatctgaag tacagttgga gctttgcaca
     8581 gagttaaagc ctgcttatca gtattaacac ctacaaactc tactccgccg atggcctctt
     8641 ccaccattct gtttactgca ttatttccgg ctccacctac accgatcact atgatcttgg
     8701 cagatgattc agcctcgttt gacataatct ctaacaacgt acttcctcct ttatttatac
     8761 ctgatatcct tttcagtgat ttatatcaaa gttacatata gatatatcat atattttttt
     8821 tgagaattaa tcaaccatta attttgtttt tttatacttt tttcactctt tttcggcaat
     8881 tccggtaaat atgttgtatc agtgtacgga gccttctctg atctcaaagt ttccatatac
     8941 tatcaatatc cctcagaatc atccccctga taatcactgt tatcttctgt ataatcacca
     9001 tatccgtctg aatcatctga gtttccgtcg tcatatgatg aatcactttc tccatcttct
     9061 gaggtttcag aactctggtc atctgcggaa ctttcatctc ctgaaccatc tcccgactca
     9121 tcagatgccg catccagaga ttcctgtgta ggcttcggtc ccacataatt accggattcg
     9181 tcatactcac ctgctccggt ataatcccct tcctcatcat aaccgccatt ccagttctca
     9241 attgcttcct caagagcagt cttcggtttt gcacctacat aattaccatc ctcgtcatac
     9301 tcgccgtctc cggtataatt gccatcttca tcataaccgc cattccagtt ctcagcagta
     9361 acttcttcct gttccagttc ttcttcaaag gtattggact ccgtagccac actctccata
     9421 tgaagaatac ctttttttcc ctgaattttg ggcagaatcg ctatcactcg gttcatcttc
     9481 tcttccagat cagcatcttt tccaagctg
//
LOCUS       DBMDXG010000031         9490 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1971_length_9490_cov_2.528917, whole genome shotgun sequence.
ACCESSION   DBMDXG010000031 DBMDXG010000000
VERSION     DBMDXG010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1971_length_9490_cov_2.528917"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(483..1730)
                     /locus_tag="ACZPD3_01880"
     CDS             complement(483..1730)
                     /locus_tag="ACZPD3_01880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1414789.1"
                     /translation="MGETVIEKIIRNNVGKTVKPGDIVTVNVDRVMIHDIFIPFVAEK
                     FEEMGFTKLWNPDKAVLIYDHLVPASQLDDTRHFHAGDAFARKYGMKNVHRSDGICHQ
                     LMTEAGYVKPGNIVFGTDSHTTTYGCVGAFSSGIGYTEMASILGTGTMWIKVPETIKV
                     VIEGELPENVMSKDIILRLIGDLGADGATYRALEFTGSTVKNMTVASRMTMANMAIEA
                     GAKCALFTPDEKTAEYCDIELNEFQKSLTGDEDATYMKTITYRAEDFVPVMACPSQVD
                     KIKNVSELEGTEIDQVFIGSCTNGRLEDLRAAAEVLKGKKVADYVKLIVTPASRKIYC
                     QAIAEGIMDTLAEAGAMITHPGCGLCCGRAGGILTDGERVVATNNRNFLGRMGTSKVE
                     IYLASPKTAAACAIAGKIVNPEK"
     gene            complement(1749..2240)
                     /locus_tag="ACZPD3_01885"
     CDS             complement(1749..2240)
                     /locus_tag="ACZPD3_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR1414790.1"
                     /translation="MEKFTGKVWVLDDDIDTDIIIPTEYLALKTIDDMKQYGFSPLRP
                     ELAGQIQKGDIIVAGKNFGCGSSREQAPEIIKALGIQCVIAKSFARIFFRNSINNGLL
                     LIEQPDLHDDIKEGDEVTVVMNEHVDYNGKQYPIASLPENLMSIIQAGGLVKAMRKLN
                     GLD"
     gene            complement(2509..3759)
                     /locus_tag="ACZPD3_01890"
     CDS             complement(2509..3759)
                     /locus_tag="ACZPD3_01890"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR1414791.1"
                     /translation="MGMTIAEKIIAAAAGVDSVKPGDIHTVKLDRLMSNDGTTHLTVD
                     MYNNKLKNPHIADTSKLVFIVDHNVPSDSPKTAASQKKMRDFAKEHNIDFWEGKGVCH
                     QVMIENYVRPGELIFGADSHTCSYGALGAFGTGVGCTDFLYGMVTGTSWVLVPESVKF
                     NLTGKLPEGVYARDLILTIIGEIGANGCNYQAMEFTGEGAKTLSISDRMALCNMAVEA
                     GAKTGIFEADEKAIEYLKEHGREPKAVYHSDPDAVYAREYTFDLSKVRPVVAKPDFVD
                     NVVPAEEACGIEINEAFLGSCNNGRIEDLRVGAEIIKGKKVAEGVRFLVVPASQTIYR
                     QALKEGLIDTFMEAGAIVMNPNCSVCWGSCQGVIGENEVLISTGTRNFKGRAGHPSSK
                     VYLGSAATVTASAITGKIALASEV"
     gene            complement(3761..4243)
                     /locus_tag="ACZPD3_01895"
     CDS             complement(3761..4243)
                     /locus_tag="ACZPD3_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR1414792.1"
                     /translation="METGTIFKFHNDLDTDQIIASQYLLLPNLDEMKGHAFESLDPDF
                     SKKVKPGDFVVGGENFGCGSSREQAPGVLKALGVQAVVAKSFARIFYRNSINIGLPVI
                     VCKDLPDEVNTGDKMVLHMSEGTVEANGKTYSCTKLPEYMQNILNQGGLIASLNKEEK
                     "
     gene            complement(4403..5593)
                     /locus_tag="ACZPD3_01900"
     CDS             complement(4403..5593)
                     /locus_tag="ACZPD3_01900"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR1414793.1"
                     /translation="MKNYDRIFVIVLDSLGIGAMPDSAKFGDIGVDTFGHILEKMGSL
                     EIPNLRKLGMLNLHPAGKMAGVENPKGRYTHLGEASNGKDTMTGHWEMMGIKTEKPFK
                     TFTETGFPPELIAELEKRCGKRVIGNKSASGTEIIEELGEEEIQTGAMIVYTSADSVL
                     QICGNEETFDLQNLYRCCEIAREITMKDEWRVGRVIARPYIGKKKGEFKRTSNRHDYA
                     LKPTGLTTLNVLKDHGFDVIGVGKIHDIFCGEGITETYHSDSSVHGMEQTIEICKRDF
                     RGLCFVNLVDFDALWGHRRNVEGYGKEIEKFDKNLGVLLEEMRENDLLILTADHGNDP
                     TYTGTDHTREYVPFIAYSKRMKNGGAIKDEDTFAVIGASVAENFGVPMPEGTIGHSVL
                     KELM"
     gene            complement(5715..6158)
                     /gene="cdd"
                     /locus_tag="ACZPD3_01905"
     CDS             complement(5715..6158)
                     /gene="cdd"
                     /locus_tag="ACZPD3_01905"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR1414794.1"
                     /translation="MQNLEKSMISKLIETAMEQLKFSYTPYSNFKVGASLLTKNGQIY
                     TGCNIENAAYTPTNCAERTAIFKAVSEGVREFDTICIVGGKDGILTEYTAPCGVCRQV
                     MMEFCDPETFRIILAIDKEHYDIYTLKDLLPLGFGLRNLINSREI"
     gene            complement(6177..6962)
                     /gene="udp"
                     /locus_tag="ACZPD3_01910"
     CDS             complement(6177..6962)
                     /gene="udp"
                     /locus_tag="ACZPD3_01910"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HIR1414795.1"
                     /translation="MKNYSEDVNRQYHIQVAKGEVGRYVILPGDPKRCVKIAQYFDNP
                     VLIADNREYITYTGTLDGVKVSVTSTGIGGPSASIAMEELYRCGADTFVRIGTCGGMQ
                     TEIKSGDIVIATAAVRMEGTSREYAPIEYPAVANLDVTNALVEAAKEKGFIYHTGVVQ
                     SKDSFYGQHEPEAMPAGYELINKWEAWKRMGCLASEMESAALFIVAGKLRARMGSCLL
                     VLANQEREKLGLENPVVHDTDMAIRVAVEAIRRMIKEDQGQVL"
     gene            complement(7387..7704)
                     /locus_tag="ACZPD3_01915"
     CDS             complement(7387..7704)
                     /locus_tag="ACZPD3_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414796.1"
                     /translation="MLEKIAAVNIGPGMEFDTSVLTGDVAENWKTMLTEIQLKLIKED
                     QKFSKKLGQWDYFGEPIGDFNTEYAYRALVALAGLGANTVEVALYPKIEQDADGNTLL
                     NFL"
     gene            complement(7995..9323)
                     /locus_tag="ACZPD3_01920"
     CDS             complement(7995..9323)
                     /locus_tag="ACZPD3_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease/transposase family protein"
                     /protein_id="HIR1414797.1"
                     /translation="MQIISSYGVELRKQNIPIRQTLEIYRSAVSYLIGIYVQVWEELA
                     EIPDAKRRFNAAEHLVHTTKKNHACFDFDIRFPKMPSYLRRSAIQHALGTVSSYKTRL
                     DLWEKTDGKSGKPKLVYENHAMPVFYRDVMYREGAEGKDEAYLKLYDGHDWKWSCVRL
                     DHTDMEYLRKCWSGKKASAPTLEKRHRKYFLRFSYKEEVTLTKTPVKEQIICSVDLGI
                     NTDAVCTIMRADGTVLGRRFIDHPSEKDRMYRTLGRIRRSQREHGSAQTQGRWAYTKR
                     LNTELGKKTAGAIVRYAEENHADVIVFEYLEMQGKISGKKKQKLHLWRKRDIQRRCEH
                     QAHRKGMRISRICAWNTSRLAYDGSGAVTRDRENHSLCTFQTGKRYNCDLSASYNIGA
                     RYFIRELLKPLPATERSLLEAKVPPVKRRTSCVYADLRKLYSEMERLKAA"
BASE COUNT         2425 a         2345 c         1557 g         3163 t
ORIGIN      
        1 acactggttc aattagttta atttatattt tgaactatac attgaaccag aatttaactt
       61 ttcttatttt agttcaatat atgttcaatt taagttttga tgtttttgaa attcctttta
      121 tttatgcgga tttctcgtca tttcgcagat tctgtttgtt caattttaag ttcaaaaagc
      181 acatgttttc gtcggagatc agactcacac tggacaattc tgtctgagaa actttttcta
      241 tgcaatcaaa tttacaggct tttccacgcc ttttctttcc ttattatccc attatcccgt
      301 cagataattt attatgtgat atgaaacata tcatagtgtc gttctctgac aaaaaataag
      361 tccactgctt actcctttta taaataatgc agtggactta tccgttaatt ttattctttt
      421 gttattctgt agtttcgttg ctcatctgta caatttatat cgcattgatt ttattgtacg
      481 ttttatttct ctggatttac aattttacca gcaattgcac acgctgctgc tgtttttggt
      541 gatgcaaggt aaatctctac cttagatgtt cccattcgac caaggaaatt tctgttattt
      601 gtagctacta cacgttcacc gtcggtaagg atacctcctg cacgtccaca gcataatcca
      661 catccgggat gtgtgatcat tgcacctgct tctgccagag tatccataat tccctctgca
      721 attgcctgac agtatatttt acgacttgca ggagttacaa tcaatttaac gtaatctgct
      781 acctttttgc cctttaacac ctctgctgca gctctgagat cctccagacg gccatttgta
      841 caggaaccga taaatacctg atctatttct gttccttcaa gctcacttac atttttaatc
      901 ttatctacct gagacggaca tgccataacc ggtacaaaat cctctgcgcg ataagtgatt
      961 gttttcatat aggttgcatc ttcatctcct gtaaggctct tctggaactc attcaactct
     1021 atatcacaat attctgcagt tttttcatcc ggtgtaaaca gcgcacattt tgcccctgcc
     1081 tcaatcgcca tatttgccat agtcatacgg cttgctactg tcatattctt tactgtactg
     1141 ccggtaaatt caagtgctct gtatgtagct ccatcagctc ccagatcacc gatcagacgc
     1201 aggatgatat ctttggacat aacattttcc ggaagttctc cctcgatcac aaccttaatt
     1261 gtctcaggaa ctttgatcca catagttcct gttcccagaa tacttgccat ctctgtatat
     1321 ccgataccgg aagagaaagc gcctacacat ccgtaggtag tggtatggct gtctgtacca
     1381 aatacaatat ttcccggttt tacataacct gcctctgtca ttaactggtg gcagattcca
     1441 tcacttctgt gaacattttt catgccatat ttcctggcaa aagcatctcc cgcatggaaa
     1501 tgtctggtat catctaactg acttgcagga accagatggt catatatgag tactgcttta
     1561 tctggattcc agagcttcgt aaagcccatt tcttcaaact tttctgctac aaatggaata
     1621 aatatatcat gaatcataac cctgtctaca ttgaccgtta caatgtctcc aggctttact
     1681 gtttttccaa cattgtttct tataattttc tcaataactg tctctcccat tttcctctcc
     1741 tttccaaatc aatccagccc attcagtttg cgcatggctt ttacaagtcc gccagcctga
     1801 ataatgctca tcagattttc cggaagagaa gcaatcgggt actgttttcc attgtaatct
     1861 acatgttcat tcattaccac agtgacttca tcaccttctt tgatatcatc atgaagatct
     1921 ggctgctcga tcagtaacag accattatta atagaatttc tgaaaaaaat tctcgcaaaa
     1981 gatttggcaa ttacacactg aattcccagt gctttgataa tttccggtgc ctgttctctg
     2041 gaagaaccac atccaaagtt ctttcctgca acaataatgt cgcctttctg aatctgtcct
     2101 gccagttccg gacgcagagg actgaatccg tactgtttca tatcgtcgat tgttttcagt
     2161 gcgagatatt ctgttggaat aataatatct gtatcaatgt catcatccag aacccacact
     2221 tttcctgtga atttttccat aatcttacct cttatcttct atcttcactt gtaactgttc
     2281 agtactttca cagtcacaag ttaaaattca ttcaaacgaa ttgtgaatat ccgctttgct
     2341 acgcggctct ataccatacc gaataactac tccgcagttt atcaggatga gcttgctctc
     2401 ctcctgatac aaacaaatct ccacctgctg tctattgctt ttcgcaacct tcgcaggaaa
     2461 tttacttgat tcggttaaat gtttcacgtg aaacatttat aattagcatc acacctcact
     2521 tgccagcgca atctttcctg taatggcaga tgctgtaaca gtagctgctg atcccagata
     2581 tactttggag cttgggtgtc ctgcacgacc tttgaaatta cgtgttcctg tactgatcag
     2641 aacttcgttc tcaccgatca caccctgaca gcttccccag catacactgc agttcggatt
     2701 cataacaatg gctccagcct ccataaacgt gtcaataagc ccctctttta atgcctggcg
     2761 gtaaatagtc tggcttgcag gcacaacgag aaatctgacg ccctcagcaa ccttcttgcc
     2821 cttgatgatt tctgcaccga cacgcagatc ttcaatacgt ccgttgttac aggatcccag
     2881 aaatgcttca ttgatttcaa ttccgcaggc ttcttctgca ggcacaacat tgtctacaaa
     2941 atcaggtttc gcaactacag gacggacttt tgataaatca aatgtatatt ctctcgcata
     3001 aactgcatcc ggatcactgt gatatacagc ctttggctca cgtccatgtt ctttcagata
     3061 ttcaatagct ttctcatctg cttcaaagat acctgtcttt gcaccggctt ctactgccat
     3121 gttgcaaagc gccatacgat cactgatact gagtgttttc gcaccctcgc ctgtaaattc
     3181 cattgcctga tagttacatc catttgctcc gatttcccca atgattgtaa gaatcaaatc
     3241 tcttgcgtaa actccttccg gaagtttacc tgtcagatta aattttacgc tctcaggaac
     3301 cagtacccat gaagtacctg ttaccatacc ataaaggaaa tctgtacatc ctacgcctgt
     3361 tccaaatgca cctaatgcac cgtatgaaca ggtatggcta tctgcaccaa agatcagttc
     3421 acccggacgg acataattct cgatcataac ctgatggcag acaccttttc cttcccagaa
     3481 atcaatgtta tgttcttttg caaaatctcg catcttcttc tgagatgctg ctgttttcgg
     3541 gctatcagat ggtacattat gatccacgat aaacaccagc ttgcttgtat ctgcaatgtg
     3601 cggatttttc aatttattgt tatacatatc aaccgtaaga tgtgtggttc catcattact
     3661 catgagacga tcgagtttaa ctgtatggat atctcccggt tttacgctgt caacacctgc
     3721 tgccgcagca ataatctttt ctgcaattgt cattcccatt ttacttctcc tccttgttca
     3781 gagatgcgat cagaccaccc tgattcaaaa tattctgcat atattccgga agctttgtac
     3841 aggaatatgt ttttccgtta gcttcaactg ttccctctga catatgaaga accatcttat
     3901 ctccggtatt tacttcgtcc ggaagatctt tacagacaat taccggcaga ccaatattaa
     3961 tagagtttct gtaaaagatt cgagcaaaag attttgctac aactgcctgt actcccaacg
     4021 ctttcagaac accaggcgcc tgctctctgg aagaaccaca tccaaagttt tctccaccta
     4081 ccacgaaatc tcccggtttt actttctttg aaaaatccgg atccagagat tcaaatgcat
     4141 gacctttcat ctcatccaga ttaggaagta acagatactg tgatgctatg atctggtctg
     4201 tatccaggtc attatgaaat ttaaaaattg ttcctgtttc cataaaaact gtttcctttc
     4261 tatatcattt tatcttttta caatttatca cttgtctctc tttaacgatt tagtcagata
     4321 aattcttttc ctattttagc acaaaagaca ggagattgaa aacgcctgtt ttcaatattc
     4381 ctgtcttttt ttacaatcgt tattacatta actcttttaa aacagaatgt ccaattgttc
     4441 cctcaggcat tggcactcca aaattctccg ccacagaagc accgatcaca gcaaaagtat
     4501 cttcgtcttt tatcgcaccg ccatttttca ttctctttga gtaagcaatg aatggtacat
     4561 attctcttgt atggtcagtt cctgtataag tcggatcatt tccatgatct gcagtgagaa
     4621 taagcagatc attctctctc atttcttcta acaacacacc cagatttttg tcaaatttct
     4681 caatctcttt tccgtaacct tccacatttc ttctgtgtcc ccataatgca tcaaaatcta
     4741 caagattaac aaaacataac cctctgaaat ctctcttgca gatttcaatt gtctgctcca
     4801 ttccatgtac agagctgtct gaatgataag tttctgtaat gccttctccg caaaaaatat
     4861 catgaatctt tccaacaccg atcacatcaa atccatgatc tttcaacacg tttaacgtgg
     4921 taagtccggt gggtttcaat gcataatcat gacggttact ggtacgttta aattcacctt
     4981 ttttcttacc aatgtacggt cttgcaataa ctctgccaac tctccattca tccttcatgg
     5041 taatttctct tgcaatttca cagcagcggt acagattctg aagatcaaaa gtttcttcat
     5101 ttccgcaaat ctgaagaaca gaatctgctg aagtataaac aatcattgca cccgtctgaa
     5161 tctcctcttc gccaagttcc tcaataatct ccgttccgct tgcgctcttg tttcctatca
     5221 ctctttttcc gcatcttttc tccagttctg cgatcaattc cggcgggaat cctgtttctg
     5281 taaaggtttt aaatggtttt tctgttttga ttcccatcat ctcccagtgt ccggtcatgg
     5341 tatcttttcc attgctagct tcacccagat gcgtatatcg ccccttggga ttctcaacac
     5401 ccgccatttt tcctgccgga tgaagattca gcattcccag ttttctaaga tttggaatct
     5461 ccagagaacc cattttctca agaatatgtc caaaagtatc caccccgatg tcaccaaatt
     5521 ttgctgaatc cggcattgcc ccgattccca gagaatcaag tacaattaca aaaattctgt
     5581 cgtaattttt catttatgtt tactccttct cttacatttt attaatcact gaccttttcc
     5641 tgttcccttt tattggtcat tcactttttt tacttttcgc aattaaaaat cacttgcaat
     5701 gatgggcgtt taccttatat ttctctggaa tttatcagat ttcgtagtcc aaatcccagc
     5761 ggtagcaaat ccttcagtgt gtagatatca taatgctctt tatctattgc cagaataatc
     5821 cgaaatgtct ccggatcaca gaactccatc attacctgac ggcagacacc acagggagct
     5881 gtatactctg tcaaaatccc atcttttcca cccacaatac aaattgtgtc aaactccctg
     5941 acgccttcgc tcactgcttt gaaaattgca gttcgttctg cacagttcgt aggtgtatat
     6001 gctgcatttt caatgttaca ccctgtataa atctgtccgt tttttgtaag taatgatgca
     6061 ccaaccttaa aatttgaata gggtgtataa gaaaatttga gctgttccat tgcagtctca
     6121 atcagtttgc tgatcattga tttttccaga ttctgcattc tctgtcacct ccgtccttac
     6181 agaacttgtc cctgatcttc tttaatcatt ctgcggattg cctctacagc tacccggatt
     6241 gccatgtctg tgtcatgtac aaccggattc tcaagaccca gcttctctct ctcctgattt
     6301 gccaatacca acagacagga acccatcctc gccctgagtt taccggctac gatgaaaagt
     6361 gccgcggatt ccatttcaga agcaagacat cccattcgtt tccaggcttc ccatttattg
     6421 atcaactcat aacctgccgg cattgcttcc ggctcatgct gtccatagaa agaatcttta
     6481 gactgtacca cacctgtatg atatataaat cctttctcct tcgcagcttc aacaagagca
     6541 ttggtcacat ccagatttgc cactgccgga tattcaattg gcgcatattc tctgcttgtt
     6601 ccttccatac gtactgctgc tgtcgcaatt acaatgtctc cactttttat ttccgtctgc
     6661 attccaccac aggttccgat tctgacaaaa gtatccgctc cgcagcgata cagctcttcc
     6721 attgcaatgg acgctgatgg gccaccaata ccggttgacg ttacactgac cttcactcca
     6781 tccagtgttc ctgtataagt gatatattcc ctgttgtctg cgatcagcac aggattatca
     6841 aaatactgtg caatctttac acatcttttc ggatctcccg gaaggatcac ataacggccc
     6901 acttcacctt ttgccacttg aatatgatac tgtctgttta catcttctga gtaatttttc
     6961 ataatcatcc catccttttc gttttcacgt gaaacatcgg ttgccactgc ttaacaatcc
     7021 tcttaattaa tgcatttaag acagttcaac aggatttcat gttctgatga gctgaatcaa
     7081 tatacattat ttacaagaat tactatagta tcttcctttt attatgatta taaatcatat
     7141 acctgtatga gaaaagttct tttttagacc tgaatccaat ttccgtccat ctgcaaatat
     7201 cttccttgtt aatttacggt tataaaaaaa ctgaggtcgt cgctccacga taaggaactc
     7261 tgacctcaat ggatttgtgg ctgttttata agttaactct cgagaggtaa ttaattctca
     7321 tattattaac tacatatctt gaataaaccg cataaataag ccattctttt gaatcgagag
     7381 ttaattttac agaaagttaa gaagagtatt tccatccgca tcctgctcta tctttggata
     7441 cagcgcaact tcaactgtat tggcaccaag ccctgcaaga gctaccagtg cacgataagc
     7501 atattctgta ttgaaatctc cgatcggttc accaaaatag tcccactgac caagcttttt
     7561 tgagaatttc tggtcctctt tgataagctt tagctgaatt tctgtcagca ttgtcttcca
     7621 gttttctgca acatcaccgg taagaacaga agtgtcaaat tccataccag gacctatatt
     7681 cacagctgct attttttcaa gcatttcctt atccagcagc tgccggagaa ttctttacca
     7741 tcaactcatt agccttatta aaaaatgtga tcggatccac attaggagtc accacagtcc
     7801 tgaattgtgc ttccgcccca aagctttatg ggtgaacctc acatgactga agtcacatgc
     7861 ttccagacgc tttaggcgtc cgactgaata tcggtggata cgcctgtaac ttaggtacgc
     7921 agttatgcgc atttcccatg ccagatatgg caggttccca cattacaggt agtccgctgt
     7981 atatctgcct gtatctatgc tgcttttaaa cgttccattt ctgaatagag tttcctcaga
     8041 tctgcataga cgcatgaggt tctacgcttt acaggaggga cttttgcctc cagtaaagac
     8101 ctttccgttg ccggaagggg ttttaaaagt tcccttataa aatatctcgc ccctatatta
     8161 taggatgctg acaggtcaca attatatcgt tttcctgtct ggaaagtaca gaggctgtga
     8221 ttttcccggt cacgtgttac cgccccggaa ccatcataag ccagtctgct ggtattccat
     8281 gcacagatcc gggatatccg catccctttc ctgtgtgcct gatgttcaca gcgtctttgg
     8341 atatcccgct ttctccacag gtgcagtttc tgttttttct ttcccgatat cttcccctgc
     8401 atctccagat actcgaacac gatcacatct gcatggtttt cttccgcata tcttacaatc
     8461 gcacctgcag tctttttacc cagttctgtg ttcagacgtt tcgtatatgc ccatcttccc
     8521 tgtgtctgcg cagagccatg ttccctctgg gatctgcgga tccgtcccag tgtgcggtac
     8581 atccggtctt tttcactggg atgatctatg aatcttcttc ccaggacagt tccgtctgcc
     8641 cgcatgatcg tacagactgc atcggtattg atccctaagt ccacgctgca gatgatctgt
     8701 tctttcacag gtgttttggt aagtgttact tcctctttat aggaaaaacg caaaaaatac
     8761 ttccggtgtc tcttttccag agtcggggca gatgcctttt tccctgacca gcattttctc
     8821 agatattcca tatccgtatg atccagacgt acacaggacc atttccagtc atgaccgtca
     8881 tagagtttca ggtatgcttc gtctttccct tccgcgcctt cacgatacat gacatcacgg
     8941 tagaagaccg gcatggcgtg attttcatat acaagctttg gtttcccgct ctttccgtcg
     9001 gtcttttccc atagatccag ccgtgtttta taagaggata ctgtccccag tgcatgctgg
     9061 atggcagatc tgcgcagata ggaaggcatc tttgggaacc ggatatcaaa atcaaaacag
     9121 gcatggtttt tcttcgtagt atgtaccaga tgttctgcag cattaaaacg cctctttgca
     9181 tccgggattt ctgctaattc ttcccatacc tgcacataaa tcccaatcag atagctgaca
     9241 gcagaacggt agatctccag tgtctggcgg atcgggatat tctgttttcg taattccaca
     9301 ccataactgg atattatctg catttttatt tttccctgat aactcttttc tcagattctg
     9361 ttgttttttc tactacccat ggctatatta cttaaatgaa aaactaattt ccagctgtgg
     9421 attatccctg taattctgca ggattaattt ccactttacg tcttaacaaa ttcatatatt
     9481 tgctttataa
//
LOCUS       DBMDXG010000032         9457 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_1981_length_9457_cov_1.926678, whole genome shotgun sequence.
ACCESSION   DBMDXG010000032 DBMDXG010000000
VERSION     DBMDXG010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_9457_cov_1.926678"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /gene="carB"
                     /locus_tag="ACZPD3_01925"
     CDS             <1..1662
                     /gene="carB"
                     /locus_tag="ACZPD3_01925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR1414798.1"
                     /translation="YKMVDTCAAEFAAATPYYYSVYGDEGTENEAVATPDKKKILVLG
                     SGPIRIGQGIEFDFCSVHCTWAFAKEGYETIIINNNPETVSTDFDIADKLYFEPLTPE
                     DVENVVNIEKPDGAVVQFGGQTAIKLTEALTKMGVKILGTSAENVDAAEDRELFDEIL
                     EKCEIPRPKGQTVFTAEEAKKAANELGYPVLVRPSYVLGGQGMRIAVSDEDVEEYIGV
                     INQIAQEHPILVDKYLMGKEIEVDAVCDGEDILIPGIMEHIERAGIHSGDSISVYPAK
                     TISEKAKETIAEYTRRLARALHVIGMINIQFIVLGDDVYVIEVNPRSSRTVPYISKVT
                     GIPIVPLATKVILGYKLKDMGYEPGLQPEAKHYAIKMPVFSFEKIRGADISLGPEMKS
                     TGECLGIAETFNEALYKAFIGAGIRLPKHKNMIITVKDEDKQDIIPIARRFEALGYRI
                     YATLGTAKVLKENGIKVIRTNKLEQPAPNLMDLILGHKIDVVIDTPPQGVEHQKDGFV
                     IRRNAIETGVNVLTSLDTAEALATSLENTDLNNLSLIDIATIARR"
     gene            1942..2850
                     /locus_tag="ACZPD3_01930"
     CDS             1942..2850
                     /locus_tag="ACZPD3_01930"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR1414799.1"
                     /translation="MDFIELLKVIFLGIVEGITEWLPISSTGHMILVDEFLKLNVTED
                     FKNLFFVVIQLGAILAVVVLYWNKLWPFYIRPISKKQQAVLNRHGAVSRGILTFVEKF
                     CDKEKWVLWFKIIVACIPTIVIALPFNDVIEEKFNNYVVVAIALIVYGILFIVIENYN
                     KRRRPTCTNLENLSFKTALIIGLFQVLSVVPGTSRSGSTIIGGILAGTSRTVAAEFTF
                     FLGIPVMFGASLLKILKFGFSFTGTEVMILIVGMVVAFVVSIIAIKFLMGYIKKHDFK
                     AFGWYRIALGILVLGYFIGKTLLGCK"
     gene            complement(2940..3878)
                     /locus_tag="ACZPD3_01935"
     CDS             complement(2940..3878)
                     /locus_tag="ACZPD3_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1414800.1"
                     /translation="MNKLYTAQEVADRLKIKKTTVYELIKRGELESSKIGKQLRVSED
                     QLTQYLNRSSSGNSESGQDIPYTSVNFEPESSLLKRDYLLHSSGIILGGQTSAALELL
                     LGKMSAHPDGLPVLQSHMNDYNGLYSLYFEKAHIAATSLDIDDIRHLVPGIPLILLSL
                     YKYSVGFYVQAGNPEKISSVENLTDPKVVFMNREKGSARRVYLDRLLKERGISSEKIS
                     GYRNEAVSDMSSASAVFEHRANVAFGEEMIARYFPGLDFVPVTDMQMYLAIPAESVRK
                     PGFSVLVEIVQSEDFKAEIHHLTGYDTSYTGEMICI"
     gene            complement(3871..4083)
                     /locus_tag="ACZPD3_01940"
     CDS             complement(3871..4083)
                     /locus_tag="ACZPD3_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR1414801.1"
                     /translation="MKLSARNQLKGKVVSINNGAVNSIVSIDIGGGNIITATISCAAV
                     EELNLKVGSDAYAVIKATNVMVGIDE"
     gene            4463..5362
                     /gene="modA"
                     /locus_tag="ACZPD3_01945"
     CDS             4463..5362
                     /gene="modA"
                     /locus_tag="ACZPD3_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1414802.1"
                     /translation="MRKKLIAAMMAGVLSAGMFSTGVFAAGTDLKGEVNTFIAASLSN
                     AMEEIQKDFNETYPDVEILYNADSSGTLQTQIEEGARCDLFFSAADKQMNALVDENLA
                     KKDTVEDILENKVVLIKPKDGETKVTGFENITDAANIALAGDSVPVGQYSREIFDNLG
                     ITDEVNKMEINEGKNVSEVLAAVSEGSNEIGIVYATDAASVADKVDVIAEAPADALKT
                     PVLYPVGLIEDKEASEDDTAATEAFLEYIKSDDAMKVFEKYGFTAYKADDASETDDKD
                     AEATEEADDTETNAETETTEEAK"
     gene            5365..6060
                     /gene="modB"
                     /locus_tag="ACZPD3_01950"
     CDS             5365..6060
                     /gene="modB"
                     /locus_tag="ACZPD3_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR1414803.1"
                     /translation="MSEFLAEINWSPLWISLKTGFTATVIAFFLGIFFARLVMKMKPF
                     SRGILDGILTMPLVLPPTVAGFILLLLFSLRRPFGAFLLDNFDIKIVQTWKGCVIAAS
                     VIAFPLMYRNARAAFEQVDVNLIAAGKTLGMSDRRIFWTVVMPTAGPGIASGTVLAFA
                     RAIGEYGATSMLAGNILGKTRTVSVAIASETAAGNYGMAGFWVVVILIISFVIVAAIN
                     IVSGKGMKMGRWM"
     gene            6060..7118
                     /locus_tag="ACZPD3_01955"
     CDS             6060..7118
                     /locus_tag="ACZPD3_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR1414804.1"
                     /translation="MAVSVDIEKKLHGFTLKVKLESDGSPMGILGASGSGKSMTLRCI
                     AGIQTPDSGRIVVNDKVLFDSEKKINLKPQERKVGYLFQNYALFPTMTVEKNIACGYR
                     GDKKHLKAKVADYIERYQLNGLEKRYPGQLSGGQQQRVALARMMIGEPEVILLDEPFS
                     ALDGYLKDIMQRDMQNFLNEYTGDMILVTHSRDEAYKFCGHLTILDSGQALTTGETKK
                     LFERPGILQAARLTGCKNFSTVQKMGKHSIYAVDWDLMLQTKDVVPDDVTHVGIRGHW
                     MKGASEGGENHMEVEVMEYIETTFEHQYLLKNKKGGDCQPVWWMCPKGNFEEDPHAKV
                     PKYIHFPEEHLMLLKEAK"
     gene            complement(7309..>7455)
                     /locus_tag="ACZPD3_01960"
     CDS             complement(7309..>7455)
                     /locus_tag="ACZPD3_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024945.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1414805.1"
                     /translation="VHRNTLVYRFEKLQRKFGLDIRAFEDALTFKLAMMVVDYIKYSK
                     NHPS"
     assembly_gap    7456..7465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7466..8235)
                     /locus_tag="ACZPD3_01965"
     CDS             complement(<7466..8235)
                     /locus_tag="ACZPD3_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HIR1414806.1"
                     /translation="MKIQQILQKCLTDWKNISQIDFCLLDSDNHIFLSTCDKKLPAES
                     KLEEFRQSSALCVSNTSCCLYKIMENHSISYILIVWGKAESTATIGELAVCQVQSLLA
                     AYAEKSDKNTFMQNLLLGSYSDVDAFNRAKKLHIATSVQRAVFLVETKQTKDENALAT
                     IRNIFSARTRDFITAIDDTGIIIIRELQSTETYEDLESIAYMLVDMLNTEAMTSAWVA
                     YSNLAEDISRLPDAYKEAHTDLEVGKIFYADKNVCGYN"
     gene            complement(8380..>9457)
                     /locus_tag="ACZPD3_01970"
     CDS             complement(8380..>9457)
                     /locus_tag="ACZPD3_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414807.1"
                     /translation="YPNGFQAVKDFNLEIEDKEFIIFVGPSGCGKSTTLRMIAGLEEI
                     SGGTLKIGDKVMNDVEPKDRDIAMVFQNYALYPHMTVYDNMAFGLKLRKVPKDQIDKA
                     VREAARILDLEKLLDRKPKALSGGQRQRVAMGRAIVRNPKVFLMDEPLSNLDAKLRVQ
                     MRIEISKIHQRLGATIIYVTHDQTEAMTLGTRIVVMKDGVVQQVDTPQNLYQKPGNLF
                     VAGFMGSPQMNFLDAVISEKGGNLIAKVGDHELAIPAAKAKALKDGGYVGKTVVLGIR
                     PEDIHDSQMFIEASPSAPMTSVVKVYELLGAEVFLYFDVNGTQVTARVDPRTTAKTGD
                     PIKFAFDMEKSHFFDKETELTICN"
BASE COUNT         2827 a         1892 c         2168 g         2560 t
ORIGIN      
        1 tacaagatgg ttgatacctg tgcagcagaa tttgctgcag caacaccata ttactattct
       61 gtatacggcg atgaaggcac agagaatgaa gcagtagcaa caccggataa aaagaaaatc
      121 ctggtacttg gttccggtcc gatccgtatc ggtcagggta ttgagttcga cttctgttcc
      181 gtacactgta catgggcatt tgcaaaagaa ggctatgaga ccattatcat caacaacaac
      241 ccggaaacag taagtacaga ctttgatatt gcagataaac tgtatttcga gcctctgaca
      301 ccggaagatg ttgagaatgt agtaaatatc gagaaaccgg acggagcagt tgtacagttc
      361 ggtggtcaga cagctatcaa attaacagaa gcactgacaa agatgggcgt taagattctc
      421 ggaacatctg cggagaacgt agatgcagca gaggaccgtg agctctttga tgagattctt
      481 gaaaaatgcg agatcccgag accaaaggga cagacagtat ttactgctga ggaagctaag
      541 aaagcagcaa atgagctggg ttatcctgtt ctggttcgtc cttcctatgt acttggtggt
      601 cagggcatga gaatcgctgt aagcgatgag gacgtagaag agtatattgg tgtgattaac
      661 cagattgcac aggagcaccc gatccttgtt gataaatacc tgatgggtaa ggaaatcgaa
      721 gtagatgctg tatgcgatgg cgaggatatc ctgattcctg gtatcatgga acatatcgag
      781 cgtgccggaa tccactccgg tgacagtatc tccgtatacc cggctaagac catcagcgag
      841 aaagcaaaag agacaattgc agaatacacc aggcgtcttg caagagcact gcatgttatc
      901 ggtatgatca acatccagtt cattgttctt ggagatgatg tatatgtgat tgaggttaat
      961 cctcgttcca gccgtactgt accgtacatc agcaaggtaa caggaattcc gatcgttcct
     1021 cttgcaacga aggtaatcct tggatacaag ctgaaagaca tgggctatga accgggactt
     1081 cagccggaag caaaacacta tgcgatcaag atgcctgtat tctcatttga gaagatccgc
     1141 ggcgctgata tcagtcttgg accggaaatg aagtcaacag gtgagtgcct tggtattgct
     1201 gagacattca atgaagcgct ttacaaagca tttatcggag caggtatccg tcttccgaaa
     1261 cataaaaaca tgattatcac tgtcaaagac gaagacaaac aggatatcat cccgatcgca
     1321 agaagattcg aagcacttgg atacagaatc tatgctacac tgggaactgc aaaagtcctc
     1381 aaagaaaacg gaatcaaagt tatccgtaca aataagctgg agcagcctgc accgaacctt
     1441 atggacctta tccttggtca taagatcgac gttgtcatcg atactccgcc acagggtgta
     1501 gagcatcaga aagatggttt cgtcatcaga cgtaacgcca tcgagacagg tgtcaatgtc
     1561 cttacttctc tggatacagc agaagcactg gcaaccagcc ttgagaatac agacctgaac
     1621 aatttatctc ttattgatat tgctacgatt gcaagaagat aaagaactga taaataatat
     1681 gaaatgatga aaagcagccg taattttctc tgtcggagaa aaggcggctg tttttatctt
     1741 atagaatcag cagaaaaata cagattacag ttgtatcagt ggcatggtgt gtaatataat
     1801 catatgagta tttacagtga gtcagataga tatatgtggg tcttttgcat tacttattat
     1861 ctggcagaaa cctgtctgat ccggttgaaa atcgttgtaa ataagaataa caacaaaaaa
     1921 gaaaacagga agggaaagat tatggatttt attgaactgt taaaggtcat tttccttggg
     1981 attgtagagg gaatcacaga atggctcccg atcagcagta ccggacatat gatcctggtg
     2041 gatgagttcc tgaaactgaa tgtgacagag gattttaaga atctgttttt cgtagtgatc
     2101 cagcttggcg cgatcctcgc ggttgtagtt ctctactgga acaaattgtg gccgttttat
     2161 atcagaccaa tttcaaagaa acagcaggca gtgcttaaca ggcatggtgc agtatccaga
     2221 ggaattctta cttttgtgga gaagttctgt gataaagaaa aatgggtact ctggtttaaa
     2281 attatagtag cctgtatacc gacgattgtt atcgcacttc cgtttaatga tgtcattgaa
     2341 gagaaattta ataactatgt agtagttgcc attgcactga tcgtatatgg tatcctcttc
     2401 attgtcattg agaattataa caagagaaga agaccaacct gtacaaatct ggaaaatctg
     2461 tcatttaaga ctgcactcat catcggtctg ttccaggtac tctccgtagt tccgggtaca
     2521 tcccgttcag gttccaccat tatcggtggt atccttgcag gaacctcccg tacagtagca
     2581 gcagaattta ccttcttcct gggaatcccg gtcatgttcg gagccagtct tctgaaaatc
     2641 ctgaaattcg gtttctcttt cacaggaaca gaggtgatga tcctgatcgt gggaatggta
     2701 gtggcatttg tagtttccat tatcgcgatc aaattcctga tgggatacat aaagaaacat
     2761 gactttaagg cattcggatg gtacagaatc gcacttggta ttctggtgct tggatacttt
     2821 atcggaaaaa cacttcttgg ttgtaaataa tgtataagaa aagccctgct gtataagcgg
     2881 catgtacgtc ataagcgtat atcccggaca gcagggcttt ttattatgtt acaggtttct
     2941 tatatacaga tcatttcgcc ggtataagag gtatcataac ctgtcagatg atgaatctca
     3001 gctttgaaat cttcagactg tacaatttcc acaagaacag agaaccccgg ctttctcaca
     3061 gattccgcag gaattgccag atacatctgc atatcagtca cagggacgaa atcaagtccc
     3121 ggaaagtaac gtgcgatcat ttcttctcca aaagcaacat tcgctctgtg ctcaaaaaca
     3181 gcagatgcag atgacatatc agagacagct tcatttctgt atccgctgat tttttcagaa
     3241 gagattcccc gttcttttaa cagacggtcc agataaaccc tgcgggcact tcccttttca
     3301 cgattcataa aaacaacttt cggatccgtc aggttctcca cagaagaaat cttttcagga
     3361 tttccggcct gtacatagaa gccgacagag tatttatata aagaaagaag tatcagcggg
     3421 attccgggaa ccaggtgacg gatatcatct atatccagac tggtagcagc gatatgtgcc
     3481 ttttcgaaat acagggaata aagtccgtta tagtcattca tatgagactg caggacagga
     3541 aggccatcag gatgagcgga cattttgccg agaagaagct ccagggcggc agaagtctgg
     3601 ccgcctaaaa tgataccact ggaatgaagc agataatctc tcttcagcag agagctctca
     3661 ggttcaaaat taacagaagt gtatggaata tcctgcccgc tttcggaatt gccggaactg
     3721 cttctgttaa gatactgtgt caactgatct tcgctgaccc tgagttgttt tccgattttg
     3781 gaagattcca gctcaccgcg cttgataagt tcatagaccg ttgtcttttt gatctttaga
     3841 cggtctgcga cctcctgagc tgtgtaaagt ttattcatca atacctacca tgacattagt
     3901 agccttgatc actgcataag catcagaacc tactttcagg tttaattctt caactgctgc
     3961 acaggagatg gttgctgtga taatgtttcc accgccgata tcaatagaaa cgatggagtt
     4021 tacagctccg ttattgatac ttactacttt cccttttaac tgattgcgtg cacttaattt
     4081 catgataatt acctcactgt ttattatttt ttttctatag atttatcgtt actgttcaca
     4141 ctccactatc ccgcgaggtg ttttggcatg gatatgccaa aatcccgaga catacaagcc
     4201 aaaattccat tgccacaggc aatctggaat gaattttggc tgcgttactg agaacggagt
     4261 gaacagtaac gatttatctg atacccgatc aaaggccgga aagttgttta tggcagtaaa
     4321 aataaggctt tcgacctttt atcatttaca tgataacata tacaaataaa attgacaaga
     4381 atacaaaagt tttgtacaat agatacaaaa ggaaacaaaa ctgaataaaa caaaacgaaa
     4441 ctaaatgaaa ggcggtaaaa atatgagaaa gaaattaatt gcagcaatga tggcaggagt
     4501 actgagtgca ggaatgttca gcacaggagt tttcgcagcg ggtacagact taaaaggtga
     4561 agtcaacaca ttcatcgcag caagcttaag caatgcaatg gaagaaatcc agaaagactt
     4621 caatgagaca tatcctgatg ttgaaatcct ctacaatgca gacagctcag gcacattaca
     4681 gacacagatc gaagaaggcg cacgctgcga tctcttcttc tcagcagctg ataaacagat
     4741 gaatgctctg gtagacgaaa atctggctaa gaaagatact gtagaagata tccttgagaa
     4801 caaagtcgtt ctgatcaaac ctaaggatgg agaaacaaaa gttacaggat tcgagaacat
     4861 cacagatgcg gccaacatcg cacttgcagg tgacagcgtt cctgttggac agtattccag
     4921 agaaattttc gacaatcttg gaattacaga tgaagtaaat aagatggaaa tcaatgaagg
     4981 taaaaatgta tctgaagttc ttgctgcagt aagtgaagga agtaatgaga tcggtatcgt
     5041 ttatgctaca gatgcagctt ccgttgcaga taaagtagac gttatcgcag aagctccggc
     5101 agatgcactg aagacacctg ttctctatcc ggtaggcctt atcgaagaca aagaggcatc
     5161 tgaagatgat acagcagcta cagaagcatt ccttgagtac atcaaatctg acgacgctat
     5221 gaaagtattc gagaaatacg gattcacagc atacaaagca gatgatgctt ccgaaacaga
     5281 tgataaagat gcagaagcaa cagaagaagc agatgacaca gaaacaaatg ctgagacaga
     5341 gacaacagag gaagctaaat aagaatgagt gaatttctgg cagaaataaa ctggagtccc
     5401 ttgtggattt cattgaaaac aggtttcaca gcgactgtga tcgcgttctt tctgggaata
     5461 ttctttgcga gactggtaat gaagatgaaa cctttttcca gaggcatact ggatggtatt
     5521 ctgaccatgc ctctggtact tcccccgaca gtggccggtt tcattctgct tttgctgttc
     5581 agtctgagac gtccgttcgg ggcatttctg ctggataatt ttgatattaa gatcgtacag
     5641 acctggaagg gctgcgtgat cgcagcatct gtaattgcgt ttccactgat gtaccgaaat
     5701 gcacgcgctg catttgaaca ggtggatgtg aatttaatcg cagcaggcaa gacacttggc
     5761 atgagtgaca gaagaatctt ctggacagtt gtcatgccga cagcaggacc cggaatcgca
     5821 tccggtacag tccttgcctt tgccagagca atcggagaat acggtgctac atccatgctc
     5881 gcaggaaaca ttcttggcaa gaccagaacc gtttccgttg ccattgcttc ggagactgcg
     5941 gcaggaaatt atggcatggc cggtttctgg gtagttgtga tcttgatcat ttcatttgtg
     6001 atcgttgcgg cgatcaatat cgtttccggt aagggaatga agatgggcag gtggatgtaa
     6061 tggcagtcag tgtggatatt gagaagaaac ttcatggctt cactctgaaa gtaaagctgg
     6121 agagtgatgg ttctccaatg gggatccttg gagcatccgg aagcggaaaa agtatgactc
     6181 tgcgctgtat tgcaggaatc cagacaccgg actcaggaag aattgttgta aatgataaag
     6241 ttctttttga ttcagaaaag aaaatcaatc ttaaaccgca ggagaggaag gtcggctatc
     6301 tttttcagaa ctatgcactt tttcctacca tgactgtaga gaagaatatc gcatgcggat
     6361 acagaggaga taagaaacat cttaaggcaa aagtggcaga ttacatagag cgctatcagc
     6421 ttaacgggct ggagaaacgt tatccgggac agctttccgg aggtcagcag cagcgtgtcg
     6481 cactggcacg aatgatgatc ggagaaccgg aagtcatcct tctggatgaa ccgttctcag
     6541 cactggatgg atatctgaaa gatatcatgc agcgtgatat gcagaacttt cttaatgagt
     6601 acacaggtga tatgattctg gtcactcaca gccgcgatga ggcttacaag ttctgcggac
     6661 atctgacaat tctggacagc ggtcaggcac ttactacagg agaaaccaag aaactctttg
     6721 aacgacccgg tattctgcag gcagccagac tgacagggtg taaaaatttc tcaactgtac
     6781 agaaaatggg aaaacacagt atttatgcag tggactggga tttgatgctg cagaccaaag
     6841 atgtggtgcc ggatgatgtt acgcatgtgg gaatccgcgg tcactggatg aaaggtgcat
     6901 ctgaaggcgg agagaaccac atggaagtgg aagttatgga atatattgaa acgacctttg
     6961 aacatcagta tttactgaag aacaagaaag gtggagactg tcagccggtc tggtggatgt
     7021 gccccaaagg aaattttgag gaagatccgc atgcaaaagt tccgaaatat attcattttc
     7081 cggaagagca tctgatgctg ctgaaagaag cgaaatagaa tatataagaa ataataaaaa
     7141 gcgttgtatt ttcaacagag atttctggtg aagatataac gcttttttaa ccgaatcaag
     7201 taagtccctg cggagccagt tattcggtgt agaatcgcgt agccacgtag ctgtaagagt
     7261 ctgccggttt gatcacgcag atggatgtta tcaaattgtc tgagaaatct atgacggatg
     7321 atttttagaa tattttatgt agtccacaac catcatcgcc agtttaaatg ttaatgcatc
     7381 ctcaaatgca cggatatcca gtccgaattt tctctgcagc ttctcaaagc ggtacaccag
     7441 agtatttctg tgtacnnnnn nnnnntggtt atatccgcat acatttttat ctgcgtagaa
     7501 aatcttgcct acttcaaggt ctgtatgtgc ttccttgtat gcgtctggaa gcctggaaat
     7561 atcctctgcc agattactgt atgcaaccca tgcggaagtc attgcttctg tatttaacat
     7621 atctacaagc atatatgcaa ttgattccag atcctcataa gtttctgtag actgtaattc
     7681 tctgatgatg ataatccctg tatcatcaat agctgtgatg aaatctctgg tacgggcaga
     7741 gaaaatattc cggatcgtag cgagagcgtt ctcatccttg gtctgtttgg tttccacaag
     7801 aaatacggca cgctgaacag aagttgcaat gtgcagtttc ttggcgcggt taaaggcatc
     7861 tacatcggaa taactaccaa ggagaagatt ctgcataaag gtattcttgt cgcttttctc
     7921 tgcataggca gccaggagac tctgtacctg gcatacggcc agctctccga tggtagcagt
     7981 actttctgct tttccccata cgatcagaat ataggagatg gaatggttct ccatgatctt
     8041 atacaggcag cagcttgtat tggaaacaca cagggcactg ctttggcgaa attcttccag
     8101 ttttgattct gcaggaagtt ttttatcgca ggtagagaga aagatatggt tatcagaatc
     8161 cagcagacag aaatcaatct ggctgatatt cttccagtct gtcaggcatt tctgtaaaat
     8221 ctgttgtatt ttcattaggt caatcctttc ttttggggta tgaatcagct tatagcaatc
     8281 cttgcaaatt gtctgatcga agtaatcagg cagccaattc tatattataa cataaaaacc
     8341 cggggaaaac tccccgggtt tattaaagat tcctgaaaat tagttgcaga ttgtaagttc
     8401 tgtttcttta tcaaagaaat gagatttttc catatcgaat gcgaatttga tcggatcacc
     8461 ggtcttagct gttgtacgag gatcaactcg tgctgtaacc tgagttccgt ttacgtcgaa
     8521 gtataagaat acttcagctc cgagaagctc gtaaacttta actacggatg tcatcggtgc
     8581 gcttggagaa gcttcgatga acatctggga atcatggata tcttccggac ggataccgag
     8641 aacaactgtt ttacctacat agccgccatc tttaagagct tttgctttag cagccgggat
     8701 agccagttca tgatcgccaa ctttagcgat aaggttgccg cctttttcgg agataacagc
     8761 gtcaaggaag ttcatctgcg gagatcccat gaatcctgca acgaataagt tgcctggttt
     8821 ctgatacagg ttctgaggtg tatctacctg ctgaacaaca ccgtctttca taacaacgat
     8881 tctggtacca agagtcatag cctctgtctg gtcatgtgtt acatagatga ttgtagcacc
     8941 cagtctctgg tggatcttgg aaatctcgat acgcatctgt acacgaagtt tagcatccag
     9001 gttggaaaga ggctcatcca taaggaatac cttaggatta cgtacgatag cacgtcccat
     9061 agcaacacgc tgtctctgac caccggaaag agccttcggt ttacgatcaa gaagtttctc
     9121 aaggtcaagg attcttgcag cttcacgaac tgctttatca atctggtctt tcggaacttt
     9181 acgaagttta agtccgaatg ccatgttatc atatactgtc atatgaggat acagagcgta
     9241 gttctggaat accattgcga tatcacggtc tttaggctct acatcgttca taactttgtc
     9301 accaatcttt aatgtaccgc cggaaatttc ttccagaccg gcaatcatac gaagtgtagt
     9361 agatttacca catcctgaag gtcctacgaa aatgatgaat tctttatctt cgatctccag
     9421 attgaaatct ttaactgcct ggaaaccatt tgggtaa
//
LOCUS       DBMDXG010000033         9064 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2085_length_9064_cov_1.978913, whole genome shotgun sequence.
ACCESSION   DBMDXG010000033 DBMDXG010000000
VERSION     DBMDXG010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_9064_cov_1.978913"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="ACZPD3_01975"
     CDS             complement(<1..136)
                     /locus_tag="ACZPD3_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HIR1414808.1"
                     /translation="MLDNYGIRTDLALEATERFTEENVEVRGVEIHEDYNEEKDIRTT
                     V"
     gene            354..617
                     /gene="rpsT"
                     /locus_tag="ACZPD3_01980"
     CDS             354..617
                     /gene="rpsT"
                     /locus_tag="ACZPD3_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR1414809.1"
                     /translation="MANIKSAKKRILVNRTKAARNKAIKSAVKTSMKKVEAAVANKDK
                     AAAEVALKDAVSTISKATSKGVYHKNNCARKVARLAKAVNSID"
     gene            complement(740..1726)
                     /gene="holA"
                     /locus_tag="ACZPD3_01985"
     CDS             complement(740..1726)
                     /gene="holA"
                     /locus_tag="ACZPD3_01985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR1414810.1"
                     /translation="MKNIQADIKSGNFKQVYLLYGEEAYLKQQYKQNLVKALNPDGDT
                     MNFNHYEGKGIDVKQLIDLCETMPFFAERRVVLLEDTGFFKNKCEELADYMKELPDYL
                     YLVFAETEVDKRNRMYKAVKACGSIAEFIRQDEKTLMRWAAGILGKAGKKITQRDMEL
                     LLTKTGTDMGNLRMEMEKLISYTEGRDVVTAEDIEEICTTQTTNRIFDMVRAVTEKNQ
                     KRALELYYDLLTLKEPPMRILFLLAKQYRQLLLAKQFAAAGLAQTEIASKLGVPGFVV
                     RNITTCARAYTISELEQAVKDFVDAEESVKTGRLEDKLSVELLIIKYSSKVK"
     gene            complement(1900..2817)
                     /gene="nadA"
                     /locus_tag="ACZPD3_01990"
     CDS             complement(1900..2817)
                     /gene="nadA"
                     /locus_tag="ACZPD3_01990"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR1414811.1"
                     /translation="MTIAEIQQEILRMKKEQDICILAHAYQGQEILEVADYTGDSYGL
                     SVQASKRDCSGVIMCGVRFMAETCKILSPKKKVWLANPVAGCPMAEQLDLEGLRELKA
                     QYPDYAVVAYINTTSELKTECDVCVTSSSAVEICKKLEQDKILFIPDPNLGHYVAEKL
                     PEKTFAFYKGGCPRHIVVSAKDVEKARAAHPDALFLVHPECRQEVAEQADYVGSTTGI
                     MEFAKKSEHKEFIIGTENSIVEHLQFECPDKKFYPVAVQLTCMNMKVTTLMDIYNCLK
                     GQGGEEIFLPENIMEGAGRCIHRMVELGG"
     gene            complement(2837..3385)
                     /locus_tag="ACZPD3_01995"
     CDS             complement(2837..3385)
                     /locus_tag="ACZPD3_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HIR1414812.1"
                     /translation="MNTAQRRTEILKLLQQEEKPVAARAMASQFGVSRQVIVQDMAVI
                     RASTPGILSTTRGYVLQHDKDIACTREFKVRHGQEHAAEELNLIVDCGGRVKNISISH
                     RVYGRVTAEMDIRSRQDVNEFVQAINNSHSSVLSSATSGYHYHLIEASSQERLDLIGE
                     QLKKAGFLAPLQPWEKEKHHNI"
     gene            complement(3460..4311)
                     /gene="nadC"
                     /locus_tag="ACZPD3_02000"
     CDS             complement(3460..4311)
                     /gene="nadC"
                     /locus_tag="ACZPD3_02000"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR1414813.1"
                     /translation="MNSITMKMQADKLIRMALQEDITSEDVSTNAVMRSAVKGTVDLI
                     AKEDGIIAGLDVYARVFQILDEKTEISFNFKDGDAVKKGDLLGTVTGDIRVLLSGERV
                     ALNYLQRMSGIATYTKQVSKLLEGSKVTLLDTRKTTPNCRVFEKYAVRIGGGCNHRYN
                     LSDGVLLKDNHIGAAGSVAKAVAMAKEYAPFVRKIEIEVETMEQVKEAVEAGADIIML
                     DNMTPEMMKEAVELIAGRAQTECSGNITKENIAKILETGVDFVSSGALTHSAPILDIS
                     MKNLHAI"
     gene            complement(4304..5593)
                     /locus_tag="ACZPD3_02005"
     CDS             complement(4304..5593)
                     /locus_tag="ACZPD3_02005"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR1414814.1"
                     /translation="MKEHYDVIIVGTGAAGLYCALNLPEKMKILLITKQEADQSDSFL
                     AQGGICMLRGEDDYENYFEDTMRAGHYENNKRAVDLMIRSSNDIIRDLLRYHVDFARD
                     GLGNLAFTREGAHSQPRILFHEDITGKEITQTLLTAAKIKENIEICEYMTMVDLISKD
                     NTVCGIIAMDRNDQAFPVYSQYVVLACGGIGGLFKNSTNFSHIAGDGVGIAMKHHVEM
                     ENLDYIQIHPTTLYSKKPGRRFLVSESVRGEGALLYDKNGQRFTNELQPRDLLSQKIF
                     AQMEKDGTEFVWEDMRPLGEKTILEHFPHIYEQCVEEGFDPRKEPIPVVPAQHYFMGG
                     IKVNLGSKTSMKGLYACGETSCNGVHGRNRLASNSLLESLVFARKAADDMIFGQTPEY
                     VRADAIDMNMYESREELLNACHETVLKEIERMKKSHE"
     gene            complement(5771..8068)
                     /locus_tag="ACZPD3_02010"
     CDS             complement(5771..8068)
                     /locus_tag="ACZPD3_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR1414815.1"
                     /translation="MRRRPVCILCMLLVVFLCVTDWLGFSLIRGNPLPQSVQTWIRKH
                     PESTICGEVVRCRENEDFQSVYLRNTYLIYNSEKVSIDNIKVYLKQKKNHSGNSDVDK
                     LLAGSLVLVSGKLEEVQSPTNPGEFDSKAYYGCQRIYYVMKKGKIKKQSQSHSVYGQF
                     LIDMQQKFAGILEKTCGMEAGAFEAIVLGDKTNLDPELKMRYQMAGIIHILAISGLHI
                     SLLGMGLYNLLKKIGLGIWPAGLLALVIMLQYGMMTGGTVSTMRAVCMFLLSVGAKIA
                     GRIYDMPTGMAAAAILILMENPAYLLDGGFLLSFGSVIGIGCVWPLVQEGMDVLNRKK
                     RSKVNEKGKIRNKLLMSFLASGVVQLTTLPIVLWLYGEVSMLGIFLNLLVLPTVGIVL
                     GSGTAGALLGLVTVRGAFLAVVPGRIILRGYEFLTVLLGRLSFCTWIGGKPEVWQIVG
                     YYLVLATAVWMYRAGVKKSENGKIFAWKIRAVYAGMVCFAILLISYRPYEDFRIACLD
                     VGQGDGIVVEIENRWNILIDGGSTNKNELGKYQLLPYLKSRGISRLDGIYVSHTDEDH
                     ISGVRELLEFVEKDLTSLRIENLILPKWSDIQENKNYRELTELAESAGVRVLTMKAGD
                     EIRYGTVRLKVLWPESTASGKEVNEDAMVLEMISKDFKGLFTGDIGMVTEEKLIQNGC
                     LEDVDFLKTAHHGSRYSTGAEFLEIVRPELAVVSCSATNTYGHPSPDTLERLKKSGSR
                     VLITRDCGAVTIVNGKSVSAFNRIK"
     gene            complement(8097..>9064)
                     /locus_tag="ACZPD3_02015"
     CDS             complement(8097..>9064)
                     /locus_tag="ACZPD3_02015"
                     /inference="COORDINATES: protein motif:HMM:NF022112.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR1414816.1"
                     /translation="YTVIGGAIVKKKLSIIITALILSILATGCSVEIHEDPGKQDAKY
                     YLYDISSDETQLQKKSYSPEETTADYMLKDMMQRMNSQQTDSQNRVSLLPEGVQMNYS
                     VEEDVLVVNFNSQYSSMSRARELLVRTGVVKTFLQVPGINSVRFTIENEDLTDSRGQA
                     VGNMTADTFAEFTGTEPDAYCSNTFTLYFTDKSGQKLVKEQRTVRYKRSIPKERIVLE
                     QLMKGPLEKGHYPTIPENTEVLNVTIADRICYVAFDGVFSSYALDVSEKIPVYSVVNS
                     LLDALDADKVQITVGGKDRLDTFGKKMELYHFYERNDKLVVKEKE"
BASE COUNT         2264 a         2349 c         1614 g         2837 t
ORIGIN      
        1 ccacagtggt acggatatct ttctcttcgt tataatcttc gtggatctcc actccccgga
       61 cttctacgtt ttcctctgta aaccgttcgg ttgcctccag tgccaggtct gttcttattc
      121 cgtaattatc cagcatctct tttccctccg tacttcattc aatatttgtt actgtttact
      181 ccgttcttat aacaatcttt tatatctatt tttagctttt tttatggaaa tatgtattga
      241 caaggaagat tatttgagat aatatatata agcatgttca taggagcgcc cgtgtctgtt
      301 tctatgacag gagtataata atattttatc gaattaaatt ggaggtgtac gaattggcta
      361 acattaaatc tgctaagaaa agaattttag taaacagaac taaagcagca agaaacaaag
      421 caatcaaatc tgcagttaaa acttctatga agaaagttga agcagcagta gcaaataaag
      481 ataaggcagc tgcagaagta gcattaaagg atgctgtttc cacaatcagc aaagctacat
      541 ccaaaggtgt ttatcataaa aacaactgtg caagaaaagt tgcccgttta gctaaagctg
      601 ttaacagcat tgactaatta tagattgaat ttttaattat aagcttatga ttaaaaagga
      661 cagccatcac cgtcatgtgg ctgtcttttt tgttattatc ttttgtaact gccagaatat
      721 ccggcagtta ccgtttttct tattttactt tactgctgta cttaatgatc agtaattcta
      781 cgctcagctt atcttccagt cgtcctgttt ttactgattc ttctgcatcc acaaaatctt
      841 ttactgcctg ttccagttct gatatagtat aggctctggc acatgttgta atgtttctca
      901 caacaaatcc gggaactccc agtttcgaag ctatttctgt ctgcgcaagt cctgctgccg
      961 caaactgttt tgccaacagc agctggcgat actgcttcgc aagaagaaac aggatcctca
     1021 tcggtggctc tttcaatgtc agcagatcat aataaagttc cagtgcacgc ttctgatttt
     1081 tctctgttac tgcacgcacc atatcaaaaa ttctgttggt tgtctgggtg gtgcagattt
     1141 cttcaatatc ctctgcagtc accacatccc tgccttctgt ataactgata agcttctcca
     1201 tctccatcct gagattcccc atatcagttc ctgttttggt cagcagaagt tccatatctc
     1261 tctgggtgat ctttttcccg gctttcccca ggatccctgc tgcccagcgc atcagagttt
     1321 tttcatcctg tctgataaat tccgcaatac ttccgcaggc ttttactgct ttgtacatgc
     1381 ggtttctctt gtctacttcc gtttctgcaa aaaccagata cagatagtcc ggcagctctt
     1441 tcatataatc tgccagttcc tcacacttat ttttgaaaaa acctgtatct tccagcaaaa
     1501 caactctgcg ctctgcgaaa aatggcatgg tttcacagag gtcgatcaac tgttttacat
     1561 caatcccctt gccttcatag tgattgaagt tcatagtatc accatccgga ttcagtgcct
     1621 ttaccagatt ctgtttatac tgttgtttca gatatgcttc ctctccatag agaagataca
     1681 cctgtttaaa atttcctgat ttaatgtctg cctgtatgtt tttcaatttt ctactcctga
     1741 ggctgttttg tctgtaactg ttcagcaatc tgttgttcta ttcacagata ccaagatatc
     1801 agactgcaga gcagttactt tgttactgtt ctcagtaacg ttacttttat tgtatcaatt
     1861 ccatgacata atagccagca gaaaatttca gattattatt cagccgccca gctccaccat
     1921 tctgtgaatg catcttccgg cgccttccat gatattttcc ggaaggaaga tttcttcccc
     1981 gccctgtcct ttcaggcagt tgtagatgtc cattaaggtg gtaaccttca tattcatgca
     2041 tgtaagctgt actgcaaccg ggtaaaattt cttatcagga cattcaaact gaagatgctc
     2101 tacaatgctg ttttctgttc cgatgataaa ttctttatgt tcagatttct ttgcaaattc
     2161 catgattcct gtagtggatc ctacataatc agcttgctct gctacttcct gacggcattc
     2221 cgggtgtacc aggaaaagtg catccggatg tgctgcgcgg gctttttcta catcttttgc
     2281 gctgaccacg atatgtctcg gacatccgcc cttataaaat gcaaaagttt tctccgggag
     2341 cttctctgcc acgtagtgtc ccaggttcgg atccggaata aataaaatct tatcctgctc
     2401 cagctttttg cagatttcaa ctgctgatga ggacgtaaca catacgtcac attctgtttt
     2461 cagttctgaa gttgtattaa tataggcgac aactgcataa tccggatact gtgcttttaa
     2521 ttcacgcaga ccttccagat ccaactgctc tgccatggga catccggcta caggattagc
     2581 cagccatact tttttcttcg ggcttaaaat cttgcaggtt tctgccataa aacgaacgcc
     2641 gcacatgatc acaccgctgc agtctctttt ggacgcctgt acactcaggc cataggaatc
     2701 tcctgtatag tctgctactt ccagaatctc ctgaccctga tatgcgtgtg caagaataca
     2761 gatatcctgt tcttttttca ttcgcaggat ttcctgctga atttctgcta ttgtcatctt
     2821 ctgaatcctt tcttacttat atgttatgat gtttttcctt ttcccagggc tgaagtggcg
     2881 caaggaaacc tgctttcttc agctgttctc cgatcagatc cagcctttcc tggctggatg
     2941 cttctatcag atgatagtga tacccggaag tagcactgct taatacactg gaatggctgt
     3001 tgttgatcgc ctggacaaat tcattcacat cctgtctgga acgaatatcc atttctgcag
     3061 tgacacggcc ataaaccctg tggctgatgc tgatattttt cacccgcccg ccacagtcca
     3121 cgatcagatt cagttcctcc gctgcatgct cctgcccatg gcgtacttta aattccctgg
     3181 tgcatgcaat atctttatca tgctgcaaca catatcctct ggtcgttgac aggattcccg
     3241 gtgtagatgc acgtatcaca gccatatcct gcacgatcac ctgtctgctt actccgaact
     3301 gtgatgccat cgccctggca gccacaggtt tttcttcctg ctgaaggagc tttaaaattt
     3361 cagttcttct ctgagcagta ttcatacttt cccatccttt ctccacgata taatcctata
     3421 gcaaattatc cgctgcaaaa cagcttcgct gccatgttat tatattgcat gaagattctt
     3481 catggaaatg tcaaggattg gagcagaatg tgtcagtgca ccgctggaaa caaaatccac
     3541 acctgtttcc aggattttgg cgatattttc ctttgtgata tttccggagc attctgtctg
     3601 tgcccggcct gcgataagct ctactgcttc tttcatcatt tccggtgtca tattgtcaag
     3661 cataataata tctgctcctg cttctactgc ttcttttacc tgttccatag tctctacttc
     3721 aatctcgatc ttacggacaa atggtgcata ttcttttgcc atggctactg ctttcgctac
     3781 gcttccggct gctccgatat gattatcctt aagcagtacg ccgtcagaaa gattatatct
     3841 gtggttgcat ccgccgccta tgcgtacagc atatttttca aaaacacggc agttcggagt
     3901 tgtctttctg gtgtccagaa gtgttacctt acttccttcc agaagctttg aaacctgttt
     3961 tgtataagta gcgataccac tcattctctg gagataattc agggcaactc tctctccgga
     4021 aagaagcacc cggatatctc ctgttactgt tccaagaaga tcgcccttct ttactgcatc
     4081 accgtcttta aagttgaaac tgatctctgt tttctcatcc aggatctgga atactctcgc
     4141 atatacatcc agtccggcaa tgattccgtc ttcctttgcg atcaaatcta cggttccttt
     4201 tactgcgctt cgcatcactg cattcgtgga aacatcctca ctggtaatgt cctcctgaag
     4261 cgccatacgg atcagcttgt ctgcctgcat tttcattgta atactattca tgactctttt
     4321 tcatcctttc aatttctttt aatacggttt cgtggcaggc attaagcagt tcttctctgc
     4381 tttcatacat attcatatca attgcatctg cccgcacata ttccggtgtc tgtccaaata
     4441 tcatatcatc tgcagccttt cttgcaaaga ccagagattc caaaagagaa ttacttgcca
     4501 gacggtttct tccatgaaca ccattacaac ttgtctctcc acacgcataa agtcccttca
     4561 tggaagtttt gcttcccaga tttaccttga tcccacccat aaaataatgc tgtgccggaa
     4621 caaccgggat tggctctttt ctcggatcaa aaccttcttc cacgcattgt tcataaatat
     4681 gaggaaaatg ttccagaatt gttttctctc ccagagggcg catatcttcc catacaaact
     4741 ctgttccatc cttttccatc tgggcaaaaa tcttctggct cagcagatct ctcggctgta
     4801 attcgtttgt aaaacgctgt ccgttcttat catacagaag agcaccttct cctctgactg
     4861 attctgaaac caggaaacgc cgtccgggtt tctttgaata gagagttgtc ggatgaatct
     4921 ggatatagtc cagattttcc atttccacat ggtgcttcat tgcaattccc acaccatctc
     4981 ccgcaatatg cgaaaagttg gtagagtttt taaaaagccc gccgattccg ccgcaggcaa
     5041 gaaccacata ctgagaataa acaggaaatg cctggtcatt ccggtccatt gcaatgattc
     5101 cgcagactgt attgtctttg gagatcaggt ctaccatagt catatattca caaatttcaa
     5161 tattttcttt tatttttgca gcagttaaga gagtctgtgt gatttctttt cctgtaatat
     5221 cctcatgaaa aagaattctt ggctgggaat gtgctccctc tctggtaaat gccagatttc
     5281 ccagaccatc ccttgcaaaa tccacatgat aacgcagaag atctctgata atatcattgg
     5341 agctgcggat catcagatct actgcccgtt tattattctc ataatgtcct gcccgcatgg
     5401 tatcttcaaa ataattttca tagtcatctt cgccgcgcag catacagatt ccgccctgtg
     5461 ccaggaatga atctgactgg tctgcctcct gcttggtgat aagcaggatt ttcattttct
     5521 ccggaaggtt cagcgcacag tacagtccgg ctgcccctgt tcccacaata atgacatcat
     5581 aatgttcttt catctttctt tcctcacatc atgcctgatt ttcgtttgtc tgtgcaatta
     5641 tactccccgg atatataggt gtcaagacac ctgtatataa ttatgacaac attgtttgct
     5701 ttgagttact gtgaacgtaa ccactttgaa aatcctactg cttattgctt ttcacatctc
     5761 cacagggggc ttacttgatt cggttaaagg cagaaacaga ttttccattg actattgtaa
     5821 ccgccccaca gtctctggtg atcagcactc tgcttccact cttcttcagc cgttccagcg
     5881 tatccggcga cggatgcccg tatgtgtttg ttgcagaaca ggaaacaaca gccagttccg
     5941 gtctgacgat ttccagaaac tctgctcctg tagaatatct ggaaccgtga tgagcagttt
     6001 tcaggaaatc cacatcttca agacacccat tctgtatcag tttttcttct gtcaccattc
     6061 cgatatctcc ggtaaaaagc cctttgaaat ctttgcttat catttccaga accatcgcat
     6121 cttcatttac ttccttccct gatgctgtgg actccggcca cagaaccttc agtctgacag
     6181 ttccatatct gatctcatct cctgctttca tagtaagcac ccggactccc gctgactctg
     6241 ccagctctgt cagctcccga taatttttat tctcctgaat atcgctccat ttgggcagga
     6301 tcagattctc tatgcgcaga gaagtcagat ctttctcaac aaattccaga agttcccgca
     6361 ccccgctgat atgatcttca tctgtgtgag aaacataaat tccgtccagt ctggaaatcc
     6421 ctctgctctt caagtatggg agcagctgat attttcccag ttcattctta ttcgtactgc
     6481 ccccgtcgat caggatattc catctgtttt ctatttccac cacaattccg tctccctgtc
     6541 ccacatccag acaggcgata cggaaatctt catacggtct gtaactgatc agcagaattg
     6601 caaagcatac catacctgca tatacagccc ggattttcca cgcaaagatc ttaccatttt
     6661 cacttttctt cacacccgct ctgtacatcc acaccgctgt tgccagaacc agataatatc
     6721 caacgatctg ccacacttcc ggctttccgc cgatccatgt acaaaacgaa agtcgcccca
     6781 acagcactgt caggaactca tatcctctca gaataatcct gcccggaaca acagccagaa
     6841 acgctcctct gacagtgact aatccaagta atgcaccggc agttccgctt cccagtacaa
     6901 tccccacagt aggaagcacc agcagattca gaaaaatccc cagcatcgac acttctccgt
     6961 aaagccacag cactatggga agtgtggtaa gctgcacaac tccggatgcc agaaatgaca
     7021 tcagaagttt atttctgatt tttcccttct cattaacctt acttcgtttt ttccggttta
     7081 atacatccat cccctcctgc actaatggcc agacacagcc aatcccaatc accgatccaa
     7141 aagacagcag aaaacctcca tcaagcagat acgccggatt ttccataagg atcaggatcg
     7201 ccgcagccgc cattcctgta ggcatatcat aaatccgtcc tgcgatcttt gcacctacag
     7261 aaagtaaaaa catacatact gcccgcatgg ttgaaacagt ccctccggtc atcatcccat
     7321 actgaagcat gatcaccagc gccagcaggc ctgccggcca gatacccaga ccgatttttt
     7381 tcagcagatt atacagtccc attccaagaa gactgatatg gagccccgaa atcgccagga
     7441 tatgtataat tcctgccatc tgataacgca ttttcaactc cggatccaga tttgttttat
     7501 ctcccagcac gattgcttca aacgctccag cttccattcc acaggtcttc tccagaatac
     7561 ctgcaaattt ctgctgcata tcgatcagga actgcccata tacagaatga ctctgcgact
     7621 gcttctttat ctttcctttc ttcatcacat aatagatcct ctggcagcca tagtatgctt
     7681 tactgtcaaa ctctcccgga ttcgtggggc tttgcacctc ttccagtttt ccggacacca
     7741 gaacaagact tcctgccaga agtttatcaa catcactgtt accgctatga ttcttttttt
     7801 gttttaaata aacctttata ttatcaatgg aaactttttc agaattgtaa ataagataag
     7861 tatttctaag atatacagat tgaaaatctt cattctcccg gcacctgacc acctccccgc
     7921 agatggtgga ttccggatgc tttctgatcc atgtctgcac agactgtggc agtggattcc
     7981 cccttatcag ggaaaatccc agccagtctg tcacacacag aaaaacaacc agaagcatgc
     8041 acaggataca cacagggcgt ctcctcactt ttgtgcctcc tcttcgttta tgaatatcac
     8101 tctttttcct tgactaccag cttatcattt ctttcataaa aatgatacag ctccatcttc
     8161 ttaccaaagg tatccagcct gtccttcccg ccgaccgtaa tctgcacttt gtctgcatcc
     8221 agcgcatcca gcagcgaatt taccacagaa tataccggta ttttctccga tacatccagc
     8281 gcataggaag aaaatactcc gtcaaatgcc acatagcaga tcctgtctgc tatggtcaca
     8341 ttcagaacct ccgtattctc cggaattgtg gggtaatgcc ctttttccag cggtcccttc
     8401 attaactgtt ccagaacgat tctctctttg gggatactgc gtttataccg cacagtacgc
     8461 tgttctttta ccagcttctg accactctta tcagtaaaat aaagcgtaaa ggtattgctg
     8521 caataggcat ccggttcagt tcctgtgaac tcggcaaagg tatctgcagt catatttccc
     8581 acagcctgtc ccctggaatc tgtcagatct tcattttcta ttgtaaaacg cacagaattg
     8641 atccccggaa cctgaaggaa agtcttcaca acacctgtcc gcacaagtaa ttccctggcg
     8701 cggctcatgc tgctgtactg actgttaaag ttgaccacca gcacgtcctc ttctacactg
     8761 taattcatct gtactccttc aggaagcaga ctgacccggt tctgcgaatc tgtctgctgg
     8821 ctgttcatcc gctgcatcat atccttaagc atatagtctg cagttgtctc ctccggagag
     8881 tagcttttct tctgaagctg agtttcatca gagctgatat cgtacagata atatttggca
     8941 tcctgtttac ccggatcttc atgtatctcc acagaacacc cggtcgcaag gatgcttagt
     9001 attaacgctg ttataatgat tgaaagtttc tttttcacaa tggcacctcc tatgacggta
     9061 tata
//
LOCUS       DBMDXG010000034         8792 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2150_length_8792_cov_1.720170, whole genome shotgun sequence.
ACCESSION   DBMDXG010000034 DBMDXG010000000
VERSION     DBMDXG010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2150_length_8792_cov_1.720170"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            300..1400
                     /locus_tag="ACZPD3_02020"
     CDS             300..1400
                     /locus_tag="ACZPD3_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414817.1"
                     /translation="MKKRYLILAGLLVMTVAAAGCGKKKTTETAPVEVTATPTPEVTK
                     AVDMVDMQQTADEDIKNVMGEKTSTASKIVFVNNTGDDIQSLYIRTHVDEDSEDYDAD
                     EDGGWGDDLINGMFTLTDKDKALYYMQTANTQTANTQTANTQTSDTQTTDTQTTSNKS
                     TASYDIRIAYADEDKNECFFRDIPLGTISQITLCMDGTDDDAIPYAKYLTGTSTKEVS
                     TLDAVKERLGITDDSESESDSTDDSDKNSADDSDSTDSNNSSDQNNNSGNGTGDSSDD
                     PGNGGNSDDPGTNDDPGSNDDPGNGGDMISTAEQYIGQSLDALEGACGSPQGSSYEDD
                     PETGKTGFHYYSNFTVSTTVDENGNEIVAGIW"
     gene            1481..2176
                     /gene="pyrH"
                     /locus_tag="ACZPD3_02025"
     CDS             1481..2176
                     /gene="pyrH"
                     /locus_tag="ACZPD3_02025"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR1414818.1"
                     /translation="MKRVLLKLSGEALAGEKKTGFDEATVIEVAKQIRTIAEEGLEIG
                     IVIGGGNFWRGRTSETIDRNKADQIGMLATVMNCIYVSDICRYLGLKTEIFTPFVCGA
                     FTSLYSKDAVEASFAQGKIVFFAGGTGHPYFSTDTATVLRAVEIEAEAILLAKAVDGI
                     YDSDPKTNPNAVKYDEISIEEVVAKKLAAMDLTASIMCMEQKMPMLVFALDEKDSIIN
                     AVHGKFVGTKVTV"
     gene            2246..2797
                     /gene="frr"
                     /locus_tag="ACZPD3_02030"
     CDS             2246..2797
                     /gene="frr"
                     /locus_tag="ACZPD3_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR1414819.1"
                     /translation="MDERIQKYEEKMKKTLASLESELVTIRAGRANPHILDKLAVDYY
                     GAPTPLQQVANITVPEARMIQIQPWESSLIKGIEKAILTSDLGLNPSNDGKVIRLVFP
                     ELTEERRKELVKDVKKKGEAAKVAVRNIRRDANDAFKKLAKQDVSEDEIKELEEKIQK
                     STDKYIKEVDAAVDAKSKEIMTV"
     gene            2912..3640
                     /locus_tag="ACZPD3_02035"
     CDS             2912..3640
                     /locus_tag="ACZPD3_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR1414820.1"
                     /translation="MRKGNKSMNVPNHIAIILDGNGRWAKAKGMPRSYGHVKGCANLE
                     TVCDYMKELGVKYVTVYAFSTENWKRSKDEVDGLMKLFRSYLKKCIKLADKNKMRVRV
                     IGEVSAFDQDIQESIARLEQYSQKYDEIYFQIALNYGSRDEIIRGIRKLAQDAADGKV
                     KPEEIDEHVFDNYLDTAGIPDPDLMIRTSGELRLSNFLLWQMAYTEFYFTDVAWPDFN
                     KAELIKAIEKYNQRDRRYGGVKEE"
     gene            3644..4438
                     /locus_tag="ACZPD3_02040"
     CDS             3644..4438
                     /locus_tag="ACZPD3_02040"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR1414821.1"
                     /translation="MFKTRLLSGILLVIIALATIISGDYVLFFTLLAVSLIGMRELYR
                     AMKVQDEKINLLAVAGYLGAVLYYLAVLLDFERYGVLAIIFGLVLLMFVYVFTYPTYE
                     AGQVMPALFGIVYVAVMLSFIYLTRELPGGKFHVWLIFLCSWGCDTCAYCVGMLIGKH
                     KMAPVLSPKKSVEGAVGGVAGAALLGVIYAAATQGPMLEYAVICAIGALISMVGDLAA
                     SAIKRNQGIKDYGKLIPGHGGILDRFDSVIFTAPVIYFLSLVMIEI"
     gene            complement(4401..4568)
                     /locus_tag="ACZPD3_02045"
                     /pseudo
     CDS             complement(4401..4568)
                     /locus_tag="ACZPD3_02045"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            4506..4679
                     /locus_tag="ACZPD3_02050"
                     /pseudo
     CDS             4506..4679
                     /locus_tag="ACZPD3_02050"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            4713..5855
                     /gene="dxr"
                     /locus_tag="ACZPD3_02055"
     CDS             4713..5855
                     /gene="dxr"
                     /locus_tag="ACZPD3_02055"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR1414822.1"
                     /translation="MKKVAVLGSTGSIGTQTLDVVRANDDLEVVGLAAGSNVEMLEKQ
                     IREFHPRLVAVWKEEAARDLAVRVQDLDVKIVSQMGGLIELARMEESDILVTAIVGMI
                     GIRPTMEAILAGKDIALANKETLVTAGHLIMPLAKQFGVQILPVDSEHSAIFQALHGE
                     KREQIHKLLITASGGPFRGKKTADLEKVTLEDTLKHPNWVMGQKITVDSATLVNKGLE
                     VMEARWLFDVDLDHIQVVVQPQSIIHSMVEFEDGAVMAQLGTPDMRLPIQYALCYPDR
                     RFLKGDRLDFHMLKQITFEEPDRKTFKGLPMAVEAARAGGSMPTVFNAANELAVRKFL
                     QKKISFLDIYEIIGQSMSRHTAVKDPDLDQILEIEKETYRWIESRW"
     gene            5857..7152
                     /gene="rseP"
                     /locus_tag="ACZPD3_02060"
     CDS             5857..7152
                     /gene="rseP"
                     /locus_tag="ACZPD3_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR1414823.1"
                     /translation="MKIIIAIVIFSAIILFHELGHFLFAKLNKIVVTEFSLGMGPRLY
                     SFEKGDTRYSLKLLPIGGSCAMLGEDTDIENEPGTFNSASVWGRISVVAAGPVFNFIM
                     AFVLSVIIVGAVGYEPSRVLSVKEGSAAEAAGLKEGDIITGYQGYHIDLGKDLYVYSY
                     LNQLKEGDTINLTVKRDGKKMDISYKSDTNVRYLLGCNFNGDDTSAMTVESVMDGMPL
                     QEAGIQQGDVITSINGVKITNAADYQKYIQENPLTEKSVKITYSRDGQEYDITVTPKE
                     YCTAESGFTYNMYSEKAKGLNIVKYGAVEVKYMVRTTILSLKELVSGKLGMKDLSGPV
                     GIVDAIGTTYEESKSEGTMILWMNMLNLAVLLSANLGVMNLLPFPALDGGRLVFLVIE
                     AIRRKPINRQVEGGIHFAGLMLLMALMVFVMYNDIVKLI"
     gene            7515..>8792
                     /locus_tag="ACZPD3_02065"
     CDS             7515..>8792
                     /locus_tag="ACZPD3_02065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR1414824.1"
                     /translation="MRKKNEITEYVAAALIFVCAVAVSLGIFLVCTQNSIERNSQKVI
                     KTNVSRQSEHIKAILDIHYGYLRGIAKEIGKSEELVSDENMEMLISLEEETALERTAL
                     IEADGTAHYDNGAEKNVSLRRYFKEGMEGKETLSDPLESSVDKETRVILGVPVWKNGK
                     VIGVLGGSYNVTALSRMLFNDFFEDVGYTLITTSDGEIIAYDGDFAYHEIEYGDNFFE
                     FYDDQTLIFDSSLTEVKKDFTVGADGLMKIRIGNDYNSDRYLAYTDMGLNDWMICYVI
                     PVSEAQKSYNFVRRYELIFTIGFCVMVSMLFLWGFVKNRSKDKQLIQAAETDALTSAY
                     NKRSTEERIHNVLQEHPQEPGTFVIMDVDHFKEVNDIYGHITGDKVLHKFGEVLHEHF
                     REGDIVGRIGGDEFVVYMRKTDSREVAVSRIESL"
BASE COUNT         2779 a         1491 c         2226 g         2296 t
ORIGIN      
        1 gtaatattcc gcgaggtgtt ttttgcgagt gaaagtcaca gtaacatcct ttctggtgag
       61 tatccgctga ttacagtgag cgtttccttt tgtgcaataa tatgaagtag gaaaaatgtg
      121 attttttcat aagaatgcgt ccgcaggcag aaactttaaa aacctgaaag tcttaaaatt
      181 gtatgaaatc ggcagaggct tagttgacaa gttttcgata ctctgcaata atagggaatt
      241 gaaaaaatga cattgttaaa aagaaaaaag atattccata tagcaggagg gaatatatta
      301 tgaagaaacg ttatttaata ctggcaggtc tgttagtaat gacagtggca gctgccggat
      361 gtggtaagaa aaagacaaca gagacagcac ctgtagaggt aacagctacc ccgactcctg
      421 aagtaaccaa ggcagtcgat atggtggata tgcagcagac agcagatgaa gacataaaga
      481 atgtaatggg ggagaaaact tccacagcat ctaagattgt gtttgtaaat aatacagggg
      541 atgacatcca gtcactctat atccggactc atgtggatga agacagtgag gattatgatg
      601 cagacgagga tggaggatgg ggagatgatc tcatcaatgg aatgtttacc ctgacagata
      661 aggacaaggc actgtattat atgcagactg caaatacaca gactgcaaat acacagactg
      721 caaatacaca gacatcagat acacagacaa cagatacaca gacaacatcc aacaagagta
      781 cagcttccta tgatatccgt attgcatatg ctgatgagga caagaatgaa tgctttttcc
      841 gcgatattcc gcttggaaca atttcgcaga ttacgttatg tatggatggc acagatgatg
      901 atgcgatccc atatgcaaaa tatctgacag gtacatcaac aaaagaagta tccacgcttg
      961 atgcggtaaa ggaacgtctt ggaattacag atgacagtga gtctgaatca gattccacag
     1021 acgacagtga taagaattct gcagatgatt cagattccac agatagtaac aacagttctg
     1081 atcagaataa taacagtggc aatggaaccg gggatagttc agatgatccg ggcaatggag
     1141 gaaactcaga cgatccgggt actaatgatg atccgggcag taacgatgat ccgggaaatg
     1201 gcggcgatat gatttccaca gcagaacagt atatcggtca gtctctggat gcactggaag
     1261 gcgcatgcgg ttctccacag ggcagcagct atgaggatga tcctgagaca ggtaaaacag
     1321 gattccatta ttacagcaat ttcacagttt ctacaacagt tgatgaaaat ggtaatgaaa
     1381 ttgttgccgg aatatggtag aatagaacta cgaatgtaac agaggaggta ttccgatcct
     1441 ctgttacctg cgataaaatt aatttcagga gacagttact atgaaacgtg tattattaaa
     1501 attaagcggt gaagcccttg ccggtgaaaa gaagaccgga tttgacgagg cgactgttat
     1561 tgaagttgca aagcagatca ggacaattgc tgaggaagga ctggaaatcg gcatcgtgat
     1621 cggcggaggt aatttctgga gaggacgcac cagtgagact atcgacagga ataaggctga
     1681 tcagattggt atgcttgcaa cagttatgaa ctgtatttat gtatctgata tctgcagata
     1741 ccttggatta aagacagaaa tctttacacc atttgtatgc ggtgcattta caagccttta
     1801 ctctaaggat gcagtggaag caagctttgc acagggcaag atcgtattct ttgcaggcgg
     1861 tacaggacat ccttacttct ccacagatac agccactgta cttcgcgcag tagagatcga
     1921 agcagaagca atccttcttg caaaagctgt tgacggaatt tatgacagtg atccgaagac
     1981 aaacccgaat gcagtaaaat atgatgaaat ttccattgaa gaagtagttg caaagaaact
     2041 ggcagctatg gatctgacag catcgatcat gtgtatggaa cagaagatgc cgatgctggt
     2101 atttgcactg gatgaaaaag acagcatcat caatgcagta catggcaaat ttgtcggaac
     2161 taaggttact gtataagtaa ggcttataag tcagtttaca gagaaagtgt aatataaaaa
     2221 ctataaaaag agggagacga acctaatgga tgagagaatt cagaaatatg aagagaaaat
     2281 gaaaaagaca ctggcaagcc ttgagagcga actggttaca attcgtgcag gacgtgcgaa
     2341 cccgcatatc cttgacaaac tcgccgtaga ttattacgga gctccgacac ctcttcagca
     2401 ggtggcaaat atcactgtcc cggaagctcg tatgatccag atccagccat gggaaagcag
     2461 cctgatcaaa ggaattgaga aagcaatcct tacctctgac cttggattaa atccaagcaa
     2521 cgatggtaaa gtaatccgcc ttgtattccc ggaacttaca gaggaacgcc gtaaggaact
     2581 ggttaaagat gtcaagaaaa aaggcgaagc agccaaagta gcagtccgca acattcgtcg
     2641 tgatgcaaat gatgcattta agaaacttgc aaaacaggat gtatctgagg atgagatcaa
     2701 agaacttgag gagaaaatcc agaagagcac agataaatat atcaaagaag ttgatgcggc
     2761 agtggatgca aagagcaaag aaatcatgac cgtataaggg aaacgaaaag gagagctcca
     2821 tgaatgcggg ctctcttttt atctgaatga gataatggac cggaaactgt ggttcattat
     2881 gatgagacag taaggttaaa ggaataacag gataagaaaa ggaaacaaga gtatgaatgt
     2941 accaaatcat attgccatta ttctggatgg taatggacgc tgggcgaagg caaagggaat
     3001 gcccagaagc tacggacatg tgaagggctg tgcaaatctg gaaacagtct gtgactatat
     3061 gaaggagctg ggagttaaat atgtgactgt atatgctttt tccacagaga actggaaacg
     3121 ttccaaagat gaggtggacg gtctgatgaa actgttccga agttatctga aaaaatgtat
     3181 taaactggca gataagaaca aaatgcgtgt ccgggtgatc ggagaggttt cggcatttga
     3241 tcaggatatt caggaaagta ttgcacgtct ggaacaatat tctcagaaat atgatgaaat
     3301 ctatttccaa atcgcattga actacggcag cagagatgag atcataagag gtatccgcaa
     3361 acttgctcag gatgctgcag atggaaaagt aaagccggaa gaaattgatg agcacgtttt
     3421 tgacaattac ctggatacgg caggaatccc tgatccggat ctgatgatcc gcacaagcgg
     3481 tgaattgaga ctttccaatt ttctgttatg gcagatggct tatacagaat tttatttcac
     3541 agatgtggca tggccggatt ttaataaagc agagctgatt aaggctattg agaaatataa
     3601 tcagagagac agaagatacg gcggagttaa ggaggagtaa agaatgttta agacaagact
     3661 cttaagcgga atcctgcttg tgattattgc gcttgccact attatcagcg gtgattatgt
     3721 gctgtttttc acacttcttg cggtttctct cattggaatg agagaattgt acagagcaat
     3781 gaaggtgcag gatgagaaga taaatctgct ggcagtagca ggctatttag gcgcagtatt
     3841 atattatctg gcagtactgc ttgattttga gaggtatgga gtgctggcaa tcattttcgg
     3901 acttgtgctg ctcatgtttg tatacgtgtt cacttatcca acctatgagg cagggcaggt
     3961 aatgcctgca ctgtttggta ttgtgtatgt ggcagttatg ttgtcgttta tttatcttac
     4021 cagagagctt cccggtggaa agttccacgt atggctgatc tttctgtgct catggggatg
     4081 tgatacctgc gcatattgtg tgggaatgct gatcggtaaa cataaaatgg cacctgtcct
     4141 cagccctaag aaatctgtgg agggagctgt gggtggtgtg gcaggagctg cacttcttgg
     4201 tgtgatctat gcagcagcca ctcagggacc gatgctggaa tatgctgtga tctgtgcaat
     4261 cggagcgctg atttccatgg taggcgatct ggctgcatct gcgatcaaga gaaatcaggg
     4321 gattaaagat tatggaaaat taattccggg acatggagga atccttgaca gatttgacag
     4381 tgtgatcttc acagcaccgg ttatttattt cctctctctt gtgatgattg agatctagag
     4441 cgtgtctgaa aaatcattcc cgcaatctgc atgccccact ttacgggata tttcacccga
     4501 attcagttgc cgtagcccgc tacggcgccc tcatccgggc aaaatatccc acaaattgtg
     4561 acgcacatct tgcagaaagc ctttttcaga cgcgctctaa cggagcatat attcttctga
     4621 tatacatgtt tacagtattt tctgatatga gaactgtcag gtcagaagta attttataaa
     4681 attgttgtat aagtattgaa atgaggtaca atatgaaaaa agtagcagtc ttaggttcta
     4741 caggttcgat cggaacacag acactggatg tggtacgtgc aaatgatgac ctggaagtag
     4801 ttggtcttgc agcaggcagc aatgtagaaa tgctggagaa gcagatacgt gaatttcatc
     4861 ccagacttgt tgcagtctgg aaagaagagg cagccagaga tctggcagta cgtgtgcagg
     4921 atctggatgt aaaaatcgta agccagatgg gtggtcttat cgaacttgcc agaatggagg
     4981 aatcagacat tctggtgacc gctatcgtag gaatgatagg aatcagacca actatggaag
     5041 caatccttgc aggtaaggat attgctctgg caaataagga aacactggta acagccggac
     5101 atttgatcat gccgctggca aagcagttcg gtgtgcagat cctgcctgta gacagcgagc
     5161 acagtgctat tttccaggca cttcatggag agaaaagaga gcagatacat aaactgctca
     5221 tcactgcatc cggcggtcct ttccgcggaa agaaaacagc agatctggag aaggtgactc
     5281 tggaggatac gctgaagcat ccgaactggg taatgggaca gaagattaca gtggattctg
     5341 caactctggt aaataaaggt ctggaagtta tggaagccag atggcttttt gatgtagatc
     5401 tggatcatat tcaggttgtg gttcagccgc agagtattat ccactctatg gtggaattcg
     5461 aggacggtgc agtgatggca cagcttggaa caccggatat gagacttccg atacagtatg
     5521 cattatgcta cccggacaga agatttttaa aaggcgacag actggatttt catatgttga
     5581 agcagatcac ctttgaggag ccggacagga aaacttttaa aggtcttcca atggcggtgg
     5641 aagcggcaag ggcaggcggc agtatgccaa ccgtttttaa tgctgcaaat gagctggcag
     5701 tccgcaaatt ccttcagaaa aaaatcagct ttctggatat ttacgagatt atcgggcagt
     5761 ctatgagcag acatacagca gtaaaggatc cggacctgga tcagattctg gagattgaga
     5821 aagaaactta tcgttggatt gaaagcaggt ggtagattga aaattattat tgcaattgtc
     5881 atattcagtg caatcattct ctttcatgag ctgggacatt ttctttttgc aaagctgaat
     5941 aagatcgtgg tcacagaatt ctctttggga atgggaccaa gactttacag ttttgagaaa
     6001 ggagatacca gatattcact gaaacttctt cctatcggtg gctcctgtgc catgctgggg
     6061 gaggatacgg acatagagaa tgaaccggga acttttaaca gtgcgtctgt gtggggcagg
     6121 atttctgtag ttgccgcagg accggtattt aactttatca tggcatttgt attgtctgtg
     6181 atcattgtag gtgctgtagg atatgaacca tccagagttc tttccgtaaa ggaaggttct
     6241 gcggctgagg cggcaggact gaaagaggga gatattatta ccggttatca gggatatcac
     6301 attgatctgg gaaaagatct ctatgtatat tcctatctga accagctgaa agagggagat
     6361 accattaacc tgactgttaa gcgtgatgga aagaagatgg acatttccta taagtcggat
     6421 acaaatgtga gataccttct gggctgcaat ttcaacgggg atgatacttc tgccatgaca
     6481 gtggaatctg taatggatgg aatgccgttg caggaagcag ggattcagca gggagatgtg
     6541 atcacatcca ttaatggagt gaagattaca aatgcagcag attaccagaa atatattcag
     6601 gagaatccgc tgacagaaaa gtctgtaaag attacctatt ccagggacgg acaggaatat
     6661 gatattacgg ttacaccaaa agaatactgt acggcagaat caggatttac ctacaatatg
     6721 tattctgaaa aagcaaaagg gctgaatata gtcaaatatg gtgccgtaga agtaaaatat
     6781 atggtacgta caacgattct cagcctgaaa gagctggtca gcggaaagct tggaatgaaa
     6841 gatctgagtg gtccggtcgg aattgtggat gcaatcggaa ctacgtatga ggaaagcaaa
     6901 agcgaaggaa ccatgatcct gtggatgaat atgcttaatc tggcagttct gctctctgca
     6961 aaccttggag ttatgaacct ccttccgttt ccggcactgg acggtggacg gctggtattc
     7021 cttgtcatag aggcaatccg cagaaaaccg attaacagac aggtggaggg cggcatccat
     7081 ttcgcagggc tgatgctgtt gatggcgctg atggtatttg ttatgtataa cgatattgtt
     7141 aagctgatct gatcatctgg cataccagtg tgggtttgaa aaaatctccc ataaaatgtg
     7201 acgcatatct tgcgtaaagt ttaaacacac tttagaacca atgtaatgta tatgattaaa
     7261 aagacagata tcttgaaaga aaatagagag tctgtctttt tttgacgaaa atttgacgat
     7321 ttatctggat tttcacagtt cgatttgata tattaaaatt gtcctgaagg tttaagcgac
     7381 agatatatgt attacacaaa tctgttacag aggattaaca gtgaaagtat tttgaccgca
     7441 gtttcgggaa agtgtgtgta taatgtaacc caacaaaaga caaacagaaa aatgtggggt
     7501 gaaaggagat gtttatgaga aagaaaaatg aaatcactga gtatgtagct gctgctctta
     7561 tatttgtgtg tgcggtagca gtaagtctgg gcatatttct ggtatgcacg cagaattcta
     7621 ttgagcgtaa ctcgcagaaa gtaattaaga cgaatgtttc cagacagagt gaacatatta
     7681 aagcaatttt ggatattcat tatggatatc tgaggggtat agcaaaagag attggaaaat
     7741 ccgaagaact tgtgtcggat gaaaatatgg aaatgttaat atcgcttgaa gaggaaactg
     7801 ctttagagcg tacagcactg attgaagcag atggtacggc tcattatgat aacggagcag
     7861 agaagaatgt atctttgagg agatatttta aggaaggaat ggagggaaaa gaaacactaa
     7921 gcgatccact ggagagcagc gtagataaag aaacccgtgt aattctgggc gtaccggtct
     7981 ggaaaaacgg aaaagtgata ggagttctgg gtggttctta taacgtaact gcattaagcc
     8041 gtatgctgtt taacgatttc tttgaagatg taggatatac actgattact accagtgatg
     8101 gtgagattat tgcgtatgat ggtgattttg cctatcatga gattgaatat ggcgataact
     8161 tctttgaatt ttatgatgat cagacactta tttttgattc gtccctgaca gaagtgaaaa
     8221 aagattttac agtgggtgcc gatggactga tgaaaataag aattggaaat gattataatt
     8281 cagaccgtta tctggcctat acagatatgg gattgaatga ctggatgatt tgttatgtta
     8341 tccctgtttc tgaagcgcag aaatcttata actttgtacg aagatatgaa ctgattttta
     8401 caatcgggtt ttgtgttatg gtaagcatgt tgttcctttg gggatttgtt aagaacagat
     8461 caaaggataa acagcttatt caggcagcag agacagatgc actgaccagc gcgtacaata
     8521 agagaagtac agaagaacgg atccataatg ttctgcagga acacccgcag gaaccgggaa
     8581 cttttgtgat catggatgtg gatcatttta aggaagtaaa tgatatatat ggacacatta
     8641 caggggacaa agtcctgcac aaatttggtg aagtgcttca tgaacatttt cgtgaaggag
     8701 atattgttgg caggattggt ggtgatgaat ttgtagtata tatgaggaaa acggacagca
     8761 gagaagttgc tgtttccaga atcgaaagtc tg
//
LOCUS       DBMDXG010000035         8735 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2163_length_8735_cov_1.894950, whole genome shotgun sequence.
ACCESSION   DBMDXG010000035 DBMDXG010000000
VERSION     DBMDXG010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8735)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2163_length_8735_cov_1.894950"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            605..1453
                     /gene="dapF"
                     /locus_tag="ACZPD3_02070"
     CDS             605..1453
                     /gene="dapF"
                     /locus_tag="ACZPD3_02070"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1414825.1"
                     /translation="MANTIIMSKYQGLGNDYLILDPKKNQVQLQGKKIALLCQRGFGL
                     GADGVLYGPVEIDGKLGVRIFNADGSESAISGNGVRIFAKYLMDNGYITEKKFDIETM
                     SGTIHVECLNSRATEFKVNIGKPSFISSDIPVSGEVREVIKENFVFHGKEYKATCLTV
                     GNPHCIIFTDNVSAEAVKNLGPYVENADEFPERMNLQICRQVDKGNLEIEIWERGSGY
                     TKASGTGSCAAAAAAYKLGLVESRINVNQPGGMIQIDMEEDETIYMTGTVGYIADISV
                     AESFFS"
     gene            1691..2801
                     /gene="prfB"
                     /locus_tag="ACZPD3_02075"
     CDS             join(1691..1765,1767..2801)
                     /gene="prfB"
                     /locus_tag="ACZPD3_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019388.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR1414826.1"
                     /translation="MVELDQFKSILATYTEPLVEVRDSLDLANKEQKVEELEREMEAP
                     DFWDDAEVSQKKMKELKDMKDDMETYQNLVTQMEDMETMIEMGYEENDPALIPEIQEM
                     LDEFQKDFDNIRIKTLLSGEYDSENAIIKLNAGAGGTEACDWCGMLYRMYSRWADKKG
                     FTLEVLDYLDGDEAGIKSVTFQVNGTNAYGYLKSEKGVHRLVRISPFNAAGKRQTSFV
                     SCDVMPDIKKDLDVEINDDEIRIDTYRSSGAGGQHINKTSSAIRITHYPTGIVVQCQN
                     ERSQHMNKDKAMQMLKAKLYLLKQQEAEEKLSGIRGEVTDIGWGNQIRSYVMQPYTMV
                     KDHRTNEETGNVDAVLDGGIDIFINAYLKWIALKK"
     gene            2958..3413
                     /locus_tag="ACZPD3_02080"
     CDS             2958..3413
                     /locus_tag="ACZPD3_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1414827.1"
                     /translation="MQKPVTEKKKYFVVRERAVPEVLLKVVEAKKLLDSGKVDTVQDA
                     AERTGISRSSFYKYKDDIFPFHEETRGKTITFIIQMDDEPGLLSVVLQTIARFHGNIL
                     TIHQSIPINGIATLTLSVDILPGEGDAEAMVETIEQNEGIHYLKILGRE"
     gene            3420..4634
                     /locus_tag="ACZPD3_02085"
     CDS             3420..4634
                     /locus_tag="ACZPD3_02085"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR1414828.1"
                     /translation="MINVAILGYGTVGSGVFEVLNENKESISRRAGQELHVKYVLDLR
                     DFPGQPVEKVLVHDYEQIVSDPEVDIVVEVMGGVEPAYTFVKKALLAGKNVCTSNKAL
                     VAKHGRELMDIAKEKSINFLFEASCGGGIPIIRVINSSLTGDEIDEVTGILNGTTNYM
                     LYKMSTEGCEFDTVLKEAQQKGYAEADPTADVEGYDACRKIAILSSLAYERYFDFEDI
                     YTEGITKITPEDMEYAAKLGRTIKLLGTSRRLADGTCYAMVAPFMLGQNSPLYSVNDV
                     FNAVFVHGNMLGDAMFYGSGAGKLPTASAVVGDIVDAAKHLHVNIVTNWNSTPAVLKP
                     LDEVTGKFFVRIKKEAAEEAKKVFGDVEIISLGQLPQECAFITDEMTQGAFEEKLAQI
                     GDQVLAKIRVKD"
     gene            4798..6006
                     /locus_tag="ACZPD3_02090"
     CDS             4798..6006
                     /locus_tag="ACZPD3_02090"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR1414829.1"
                     /translation="MLIVKKFGGTSVANKERIFNVASRCIEEYRKGNDVVVVLSAMGK
                     YTDELITMARDVNEKPPKREMDMLFTIGEQMSVALMAMAMDKLGVPAVSLNAFQVSMH
                     TTSSHGNARLKRIDTERIRRELDSKKIVIVTGFQGVDKYDDYTTLGRGGSDTTAVALA
                     AALHADACEIYTDVDGVYTADPRKVPKARKLKEITYDEMLDLATLGAGVLHNRSVEMA
                     KKYGVPLVVRSSLNNSEGTVVKEEVTVERMLISGVALDTDAVRIAVIGLKDEPGVAFK
                     LFDTLAKKNINVDVILQSIGRAGRKDISFTVDGNDLDDAVAVLDANQARLGFAELHSE
                     RNIAKLSIVGAGMMSNPGVAAKMFESLYNEGVNINMISTSEIRVTVLINEKDGVRAMN
                     AVHDAFGLAD"
     gene            6021..7103
                     /locus_tag="ACZPD3_02095"
     CDS             6021..7103
                     /locus_tag="ACZPD3_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR1414830.1"
                     /translation="MTSRRELKNYAKQAMSGKYGILILAFVAVQALALISSMISSALF
                     PGEETIDIVLSYAFTFILTLLINVVAAGMNYMYLNIARGKAYSLNDLFYFYRHHPDRV
                     IVAGFFMAVLNLLTMLPYTIYSNANLPGEDASVEVLITWLYTGVVLMIVGMIVYQILV
                     IPLEMTYYILADKPELKSTEAMKESLEMMHGNFGRYLMLKISFIPLMFLSVFTFYIAL
                     LWIFPYMAMTEVMFYRDLTGELKVQKEEEERVARDYVNPMFDSYSQSEQPQEDQTHPQ
                     QFWSVTGDSSVASDENKEVQNNAASENTQDSLYGSVENNRTTQDNPYDSSESKEVQDS
                     LQSQTIPEEEEDNEPKPWDEYFNSLK"
     gene            complement(7239..8135)
                     /locus_tag="ACZPD3_02100"
     CDS             complement(7239..8135)
                     /locus_tag="ACZPD3_02100"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1414831.1"
                     /translation="MSDFRLKVFRSVAKNLSFTKASQELFVSQPAITKHIQELEAAYQ
                     TRLFDRQGSRISLTESGNLLLEHCERILEDYKRLEYEMHLLHNEYTGGLKLGASTAIA
                     QYVLPPLLASFIGKFPQVSLSLLNGNSREIEAALQEHRIDLGFVEGVFRLPNIRYTTF
                     LEDELVAVVRTGSKLAVGEEITPDELFHLPLVLRERGSGTLDVFERALQQHNIKLSSL
                     QVLMYLGSTESIKLFLEHTDCLGIVSVRSITRELYSGQLRVVEIKGMPMLRDFSFAQP
                     QGQESGLAQVFMQFAMHNNHKL"
     gene            complement(8156..>8735)
                     /locus_tag="ACZPD3_02105"
     CDS             complement(8156..>8735)
                     /locus_tag="ACZPD3_02105"
                     /inference="COORDINATES: protein motif:HMM:NF042670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6621 family protein"
                     /protein_id="HIR1414832.1"
                     /translation="VIDVAYLNFVINDLRKYFEPLLGRSLQTVDLALFTMYLAMDAGL
                     KGSDNDMQVLLVYDKQSGKLEHCLPSDLKNELDGVAFKASLGEFSFMAVPSEGFVSRG
                     DLYLDLLQIVLNAAEVKKLIVVPFNEEYGKEVENVLRECALENAGHKEEAKDIVYFRM
                     EEPAAPAVCRWEMLGYPLMSVLGIRSEDLQNN"
BASE COUNT         2763 a         1600 c         2061 g         2311 t
ORIGIN      
        1 atctgcaatg attatttaat cgaatcaagc aagtccccgg tggagattgt gaaaagaaat
       61 aagcagtgga aagcaataag cggagggaaa agtttgattc atggtataat catatcattt
      121 aatgcgcaaa tgtacaatta ttactaagaa tgtaacaaat agtaacgaat atattttata
      181 atttctgaga aaatatgcta caaagatatt gacattatta cacgataata gtatcataca
      241 attacaaagt aatcgtaaaa actttgtaac tatttcgaaa tagggtaaag aaatagaaaa
      301 ctgaacagag aggagtgatg ttgctgtaag gctgttatat cagagaactg atataatggt
      361 cttttttgta atacctacag agcatttttt gcaatgtttt aagcatttta ctctatatac
      421 atggtaaaaa caataaaata taatgaatat aaagtcagga tgtgatatga gaaaattacc
      481 tgacacaaaa tttaatcaga caagtggcga agtgagttgg gaagattgcc cgacaattga
      541 ttatcgatgc agatacaaaa gcgggaagta tccgggatta taaatcagaa ggagagatat
      601 ggttatggca aatacaatta ttatgagtaa atatcaggga cttggaaacg attatctgat
      661 tcttgatccg aagaaaaatc aggtacagct tcagggaaag aagattgcac ttctctgcca
      721 gagaggattt gggcttggcg ctgacggtgt gctctatgga ccggtagaaa ttgatgggaa
      781 attgggtgtg cgtattttta acgcagacgg aagtgaatct gccatcagtg gaaacggcgt
      841 gcgtattttt gccaaatatc tgatggataa tggatatatt actgagaaaa aatttgacat
      901 tgaaactatg tcaggcacga ttcatgtgga atgcctgaac agccgggcaa cggaatttaa
      961 agtaaacata ggaaaaccat cttttatctc cagcgatatc cctgtttccg gtgaagttcg
     1021 tgaagtaatt aaggagaatt tcgtattcca tggaaaagaa tacaaggcta cctgcctgac
     1081 agttggtaat ccacactgca ttatttttac agacaatgta agtgcagaag ctgtaaagaa
     1141 tctgggacct tatgtggaaa atgcagatga atttccggag agaatgaacc tgcagatctg
     1201 ccgtcaggtc gacaagggaa atctggagat tgaaatctgg gaaagaggct ccggatatac
     1261 aaaggcttcc ggaacaggaa gctgtgcggc agcagcggca gcctataagc tgggactggt
     1321 ggaatcccgt attaatgtaa atcagccggg cggaatgata cagatcgata tggaagagga
     1381 cgaaactatc tatatgacag gaacagtggg atatattgca gatataagtg ttgcagagag
     1441 cttcttctca taaattacac agactttatg cgtaattcag tgtaaagtat gattacccac
     1501 agtgtgaaat gattgttacg ataaatgatc atttcacgct gtgggtaatt ctttgtttgt
     1561 aatttgctta aagatatgtt attattttaa acgaattatt tatcaggact gctataatct
     1621 atagcaattc agtcggttat ccacagggag tccgacagag aatatttata ataagaaagt
     1681 gggtgaagct gtggttgaat tagaccagtt caagagcatt cttgcgactt atacagaacc
     1741 attagtggaa gtgagggatt cactttgacc tggctaacaa agagcagaag gtcgaagaat
     1801 tagagcgcga aatggaagcg cctgatttct gggatgatgc agaagtttcc cagaagaaaa
     1861 tgaaagaatt aaaagacatg aaagatgaca tggagaccta tcagaatctg gttacacaga
     1921 tggaagatat ggaaaccatg atcgagatgg gatatgagga gaacgatcct gcactgattc
     1981 ctgagattca ggaaatgctg gatgagtttc agaaggattt tgacaatatc cgtatcaaaa
     2041 cacttctctc cggagaatat gacagtgaga atgctatcat taagctgaat gcaggtgcag
     2101 gcggaacaga ggcctgtgac tggtgcggta tgctttaccg tatgtacagc cgctgggcag
     2161 ataagaaagg atttaccctg gaagtgctgg attatctgga cggcgatgag gcaggtatca
     2221 aatctgttac tttccaggta aacggaacaa atgcttatgg ttatctgaaa tcagagaagg
     2281 gagttcaccg tctggtgcgt atttctcctt tcaatgcagc aggtaagcga cagacatctt
     2341 ttgtatcctg tgatgttatg ccggatatca agaaggatct ggatgtagag atcaatgacg
     2401 atgagatccg tattgatact tatcgaagca gtggtgccgg cggtcagcat atcaacaaga
     2461 catcatctgc catccgtatt acacattatc ctaccggaat cgtggtacag tgtcagaatg
     2521 aacgttctca gcacatgaat aaagataaag ccatgcagat gctgaaagcc aagctgtatc
     2581 ttctcaagca gcaggaggca gaggaaaaac tctccggtat ccgcggtgag gtgacagata
     2641 tcggatgggg aaatcagatt cgttcttatg ttatgcagcc atataccatg gtaaaggatc
     2701 atcgtacaaa cgaggagacc ggaaatgtgg atgcagtgct tgacggagga attgacattt
     2761 tcatcaatgc atatctgaaa tggatcgcac tgaagaaata aaagaataaa aaaggctgtc
     2821 tgaaatcagg acagcctttt tcgtgtgtgc gccataatga ttctgcagaa gatatttctt
     2881 gcgtattttt gtgtggtgtg ataatatatc cagtgtgtaa aaacaaatgt ctttaaagaa
     2941 cggacaggta aattgatatg cagaaaccag taacagagaa aaagaaatat tttgtagtga
     3001 gggaacgagc agttccggaa gtcctgctga aggtggtgga ggcaaagaag cttctggact
     3061 ccggcaaagt ggacactgta caggatgcag cagagcgcac aggaatcagc agaagctctt
     3121 tctacaaata caaagatgat atttttccat ttcatgaaga gacaagggga aagacgatta
     3181 cctttattat ccagatggac gatgaaccgg ggcttttgtc ggttgtcctg cagacgattg
     3241 cacggtttca tggaaacatt ctcaccattc atcagagtat tcctatcaat gggattgcca
     3301 cacttactct gagtgtggac attcttccgg gcgaggggga tgcagaagca atggtagaga
     3361 cgattgaaca gaatgaagga attcattatc tgaaaatatt aggcagggag taaaagaaaa
     3421 tgatcaatgt agcaatttta ggatatggca cagttggaag cggtgtattt gaagtattaa
     3481 atgaaaataa agagagcatt tccagacgtg ccggacagga acttcatgta aaatatgttc
     3541 tggatcttcg tgattttccg ggacagccgg tagagaaggt tctggtccat gattatgaac
     3601 agatcgtcag tgatccggaa gtagatattg tagtagaggt tatgggcggt gtggagcctg
     3661 cttatacttt tgtaaagaag gcacttctgg caggaaaaaa tgtctgcact tccaataagg
     3721 ctctggttgc aaagcatggc cgcgagctga tggatattgc aaaggaaaag agcattaact
     3781 tcttatttga agcaagctgt ggcggcggaa tcccgatcat tcgtgtcatc aattcttccc
     3841 ttacaggaga tgagattgac gaagtaacag gtatcctgaa cggaactacc aactacatgc
     3901 tttataagat gagcacagaa ggctgtgaat ttgacactgt attaaaggaa gcacagcaga
     3961 agggttatgc agaggcagat cctaccgctg acgtggaagg atatgatgca tgccgtaaga
     4021 tcgcaatcct ttcctctctt gcttatgaga gatattttga ttttgaagat atttatacag
     4081 agggaattac aaagatcaca cctgaagata tggagtatgc tgcgaagctt ggcagaacca
     4141 tcaaacttct tggaaccagc cgcagactgg cagacggaac ctgttatgca atggtagcac
     4201 cgtttatgct cggccagaac agccctcttt acagtgtaaa tgatgtgttt aatgcagtat
     4261 ttgtacatgg aaatatgctt ggagatgcca tgttctatgg aagcggtgca ggtaaactgc
     4321 ctacagcaag tgctgtagtt ggtgatattg tggatgccgc aaaacatctc catgttaata
     4381 ttgtgacaaa ctggaacagc actccggcag tactgaaacc actggatgaa gtgacaggaa
     4441 agtttttcgt gagaatcaaa aaagaagcag cagaagaagc gaagaaggta tttggtgatg
     4501 ttgagatcat cagcctggga cagcttccac aggaatgtgc atttatcact gatgaaatga
     4561 cacagggagc atttgaagag aaactggctc agatcggaga tcaggtactt gcaaagattc
     4621 gagttaaaga ttgataaact gacagatttc gtgtatcata tagtgtgcct gataaggtgg
     4681 acactgatac aatacttcag ataaatattt gtacagtgat tactgtaaac agaacaggaa
     4741 agggaagata tgagaatctt ctctttcctg taaacttatt atggaggaag taaaacaatg
     4801 ttgattgtca agaaatttgg cggtacatcg gttgcaaata aagaacgtat ctttaatgtt
     4861 gccagccgct gtatagaaga gtacagaaaa ggaaatgacg tggttgtcgt tctgtccgca
     4921 atggggaaat atacggatga actgatcact atggcaaggg atgtcaatga gaaaccgccg
     4981 aaaagagaga tggatatgct ctttaccatc ggagaacaga tgtctgttgc actgatggca
     5041 atggcgatgg ataaactggg agttcctgcg gtttccctga atgcttttca ggtttccatg
     5101 catacgacaa gcagtcatgg aaatgcacgt ttaaaaagaa ttgatacaga gcgtatcaga
     5161 agagagctgg acagcaaaaa gattgtaata gttaccggct tccagggagt tgacaagtat
     5221 gatgattata cgactcttgg aagaggcggt tctgacacaa cagcggttgc tcttgcggct
     5281 gctcttcatg cagatgcctg tgaaatctat acagatgtag atggtgttta cacagcagat
     5341 ccgcgtaagg ttccgaaagc aagaaagctc aaagagatca cttacgatga aatgcttgac
     5401 cttgcgactc tcggggcagg tgtgcttcac aaccgttctg tggagatggc gaagaaatat
     5461 ggcgtgccgc ttgtggtacg ttcaagttta aacaacagtg aaggaactgt ggtaaaggag
     5521 gaagttacag tggagagaat gttgatcagc ggtgttgcgc tggatacaga tgcagtgaga
     5581 attgcggtaa tcggattaaa ggatgagccg ggtgtagctt ttaaattatt tgataccctt
     5641 gcgaagaaga atattaatgt agatgtgatc ctgcagtcta tcggacgtgc gggcagaaag
     5701 gatatttctt tcacagtcga tggaaatgat ctggatgatg cagttgcagt tctggatgca
     5761 aatcaggcaa gacttggatt tgcagagctt cattctgaga gaaatattgc gaagctttcc
     5821 atcgtaggtg caggaatgat gagtaatccc ggagtagctg caaagatgtt tgagagcctc
     5881 tataatgagg gcgtaaacat caatatgatc tctacatccg agattcgtgt gacagttctg
     5941 atcaatgaaa aggacggcgt gcgcgcaatg aatgcggtac atgatgcctt tggtctggca
     6001 gactgattta ggagaattac atgacatcga gacgtgaatt aaaaaactat gcgaagcagg
     6061 ccatgtcagg aaaatatggg atactgatcc tggcatttgt tgcagttcag gcgctggccc
     6121 tgatcagcag catgatatct tcggctttat ttccgggaga ggaaacaatt gatattgtgt
     6181 tgtcgtatgc atttacattt attctgactc tgctgatcaa tgtggtagcg gcaggtatga
     6241 attatatgta tctgaatatt gccagaggga aagcatattc attaaatgat ctgttttatt
     6301 tttacagaca tcacccggac agagtgatcg ttgccggatt ttttatggca gtactgaatc
     6361 tgcttaccat gctgccctat acgatttaca gtaatgcgaa tcttccgggt gaagatgctt
     6421 ctgtggaggt gctgatcacc tggctgtata caggtgttgt tctgatgatc gttggaatga
     6481 ttgtttatca gattctggtc attcctctgg agatgactta ttatattctg gcagataagc
     6541 cggaattgaa aagtacagag gctatgaaag agagtctgga gatgatgcat ggaaacttcg
     6601 gaagatatct gatgctgaag ataagcttta tcccattgat gtttctttct gtttttacat
     6661 tctatattgc actcttgtgg atatttccat atatggccat gacagaagta atgttctatc
     6721 gtgatctgac aggggagctt aaagtgcaga aagaggaaga agaacgtgtg gcacgagact
     6781 atgtgaatcc tatgtttgac agttattcac agagtgaaca gccacaggaa gatcagacgc
     6841 atccgcagca gttttggagt gtgacaggtg attcatcagt ggcttcggat gagaacaaag
     6901 aagtgcagaa taatgcagca agtgagaata cccaggatag tttgtatggt tctgtggaaa
     6961 ataatagaac gacacaggat aacccatatg attcttcaga gagtaaagaa gtacaggaca
     7021 gcctgcagag tcagacaatt ccggaagagg aagaagataa cgaaccaaaa ccatgggatg
     7081 aatacttcaa tagcttgaaa taaaatcata atatgaaaga cctttaactg acacaatgta
     7141 aaaatgttga taaaggtctt ttttattttt attaatttgt tttcgaccgc aaaggtaggc
     7201 ttcttttctc aatcatgcca attgtttctg cttatgtgct ataacttatg gttattgtgc
     7261 atggcaaatt gcatgaatac ttgcgccaaa ccgctttctt gcccctgagg ctgtgcaaaa
     7321 ctaaaatcgc gtaacatagg cattccctta atctcaacga cacgcaactg tccagaatac
     7381 aactcgcgcg taatggaacg aacggaaaca atgcccagac aatcggtatg ttccaaaaac
     7441 aatttgatac tttccgtgct tcccaaatac atcaaaacct gtaaagagga taactttata
     7501 ttatgttgct gcaaggcacg ctcaaaaaca tccaatgtcc ccgaacctct ttcgcgaagc
     7561 accaaaggca aatggaacaa ttcatcagga gttatctcct ctcccacagc caatttactg
     7621 cctgtacgca ccactgccac cagctcatct tccaaaaaag tagtatatct aatattaggc
     7681 aacctgaata ctccttcgac aaagcccaaa tcaatgcgat gttcctgcaa agcggcctct
     7741 atctctctgg aattaccatt caacaatgac aggctgactt gtgggaactt accgataaag
     7801 ctggcaagca aaggaggtaa cacatattgg gcaatggcag tgctggcccc cagcttcaaa
     7861 ccgcctgtat attcattatg caacaagtgc atttcgtact ctaaccgttt atagtcttcc
     7921 aaaatccgct cgcaatgttc caagagcaaa ttgcccgact ccgtcaacga aattctgcta
     7981 ccctgccggt cgaacaaacg tgtctgataa gcagcttcca actcttgtat atgtttggta
     8041 atagcaggct ggctgacaaa caattcttgc gaagccttcg taaaactcag attcttcgca
     8101 acagaccgga atacctttaa acgaaaatcg ctcacagcac aatgaattaa attgattaat
     8161 tattctgcaa atcttcactc ctgataccta acactgacat cagcggatac cccagcatct
     8221 cccaacggca tacagccgga gcggcaggct cctccatacg gaaataaaca atgtcttttg
     8281 cctcttcttt atgtccggca ttttccaatg cgcattccct caaaacgttc tcaacctctt
     8341 taccatactc ctcattaaaa ggaacaacaa tcagcttctt cacttcggcg gcattcaata
     8401 ctatctgcaa caaatcaaga tacaagtctc cacgagacac aaaaccctct gaaggcacag
     8461 ccataaaact gaactcaccc aaagaagcct taaacgctac tccatccaat tcgttcttca
     8521 aatcggatgg caggcagtgc tccagctttc cggactgctt atcatatacg agaagtacct
     8581 gcatatcatt gtcactccct ttcaatccgg catccatagc cagatacatg gtaaaaagcg
     8641 ccagatccac tgtttgtaaa gaacgtccca gcaacggttc aaaatacttc ctcaaatcgt
     8701 ttataacaaa attcaggtag gcaacatcaa tcacc
//
LOCUS       DBMDXG010000036         8734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2164_length_8734_cov_2.000232, whole genome shotgun sequence.
ACCESSION   DBMDXG010000036 DBMDXG010000000
VERSION     DBMDXG010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_8734_cov_2.000232"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="ACZPD3_02110"
     CDS             <1..213
                     /locus_tag="ACZPD3_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HIR1414833.1"
                     /translation="VYRFGVRIYYGAPSKKEFQTIVKALAERNGITMPEDELLLEANK
                     WELSHGGLTGRTAQQFIDHLLGVEKE"
     gene            250..1788
                     /locus_tag="ACZPD3_02115"
     CDS             250..1788
                     /locus_tag="ACZPD3_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR1414834.1"
                     /translation="MAVMTFAAIDIGSYEVSMKIFEMSKRIGFRELNDVRYSLEIGKG
                     VYSDGKIDSEMLNVLCEVLNDFKRLMQDFGVEEYRACGTSAFRELVNPLLIIEQIYQR
                     TGMKIEILSSAEQHFLGYKSIAAIEKGFKKMIQKGTAILDVGGGSLQVSLFDKDALVT
                     TQGLKMGSLRIRQRLQELEKTTIHYDKLVEEFIRNDLMSFQRLYLKDKEIRNVILMGD
                     FITDMIFQEEMEDRIITREEFMKRYEDTVGKSVDLLAQEMEIDPEYASLVVPTMVVCR
                     NFIDIFNAESLWAPGVSLLDGIAYDFAEKKKFLKSVHNFENDILVTSKNIAKRYSSSK
                     SHIQGTMNLCLNIFDSMKKVHGMGARERLLLQIAALLHDCGKYISMEKVSECSYQIIM
                     STDIIGLSFMERQMIACAVRFNNSAFVYYDELYSMGIAIDRESYIKVAKMTAILRLAN
                     AMDRSHYQKVKGIKAVLKDRELQMIVDSAQDISLELGLLKDKEAFFEEVFGIRLVLRR
                     KGRA"
     gene            1803..3971
                     /locus_tag="ACZPD3_02120"
     CDS             1803..3971
                     /locus_tag="ACZPD3_02120"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR1414835.1"
                     /translation="MDFEKFEKKEYFVNRELSWLKFDERVLSEARDKNLPLFDRLKFL
                     SITASNLDEFFMVRVASLKDQVHAGYHKTDIAGMTAKEQLKEISVRTHELVHVQYNTL
                     NRSLIPALEKAGMHLVAAHENLTEAQSVFVDRYFEDNVYPVLTPMAMDSSRPFPLIRN
                     KTLNIGALISKKEKSDKLSKKDKTGELLFATVQVPSVLPRVVQIPSKKDGDTTVILLE
                     EIIERNIDKLFLSYDVICAHPYRIMRNADLTIDEDEAEDLLVEIQRQLKKRQWGEVIR
                     LEVEDKMDERLLKILKTEFDIKEADIFEINGPLDLTMLMKVYGADGFDAYKTPRYQPA
                     PVPEFQNEKDIFQVIREGDVFLHHPYMSFDPVVNFVRQAAKDPDVLAIKQTLYRVSGN
                     SPIIAALAQAAENGKQVSVLVELKARFDEENNIVWAKKLEKAGCHVIYGLVGLKTHSK
                     ITLVVRREETGIRRYVHLATGNYNDSTAKLYTDCGIFTCDERFGEDATAVFNMLSGYS
                     EPKSWNKLIVAPIWMKDRFLSLIEREAENAKKGLPALIRAKMNSLCDPKIIAGLYYAS
                     SCGVQVELLVRGICCLKVGVPGISENIHVRSIVGEFLEHSRIFYFENGGNPEIYMGSA
                     DWMPRNLDRRVEIVFPVEDEKIKKELEHVLDLEFKDNVKAHILQPDGTYVKPDKRGKA
                     QINSQMEFCIEATEKAALHKHEEKKSRVFIPAEPAEDIEE"
     gene            4227..4298
                     /locus_tag="ACZPD3_02125"
     tRNA            4227..4298
                     /locus_tag="ACZPD3_02125"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4260..4262,aa:Glu,seq:ctc)
     gene            4440..5006
                     /locus_tag="ACZPD3_02130"
     CDS             4440..5006
                     /locus_tag="ACZPD3_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1414836.1"
                     /translation="MERKNDMVTDEELYGQYLCGDETGLELLIKKYGDPLTLYIDGYL
                     HDVHEAEDLMMETFSWLFMKKPRIRNGCFKAYLYKAARHMALRHKSRRRIIFSLDNLT
                     REAEAQTLVEEVVRTKERNQILHLCMDELNPDYREALYLTYFEGMSYQQAAEVMGKSV
                     KQITNMVYRGKERLRGLLKREGITNAER"
     gene            4993..5361
                     /locus_tag="ACZPD3_02135"
     CDS             4993..5361
                     /locus_tag="ACZPD3_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HIR1414837.1"
                     /translation="MLKDEERIAEVKRRIAKKEQQQRLRRRRIISAVCIAACFAVIVG
                     VSFAMPGIVGQIEPGTSSGFETAATILGGSTALGYIVIGLLAFVLGACVTILCFRIHQ
                     LNKEEQTEKQKEDNGDGADQ"
     gene            5345..5971
                     /locus_tag="ACZPD3_02140"
     CDS             5345..5971
                     /locus_tag="ACZPD3_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR1414838.1"
                     /translation="MELISNLFQFAVTLLGFCMSGIRYLKGRKQTYFLLTCFYGCFAL
                     GSLYWTLYLLLFSETPQVFYVSEFGWIASVIFLYLLQYTLSSAEERDFSTRKSLIAPL
                     IGVPLCVFYCTFGDVLSNLLWCGMMIVVSYHSIRGLAYAQIQTGTACKMRYFHIGVLC
                     YVAVEYVLWISGCLWPGYSISAPYCWLDLLLTGCLFALLPATGKAVQV"
     gene            5968..6591
                     /locus_tag="ACZPD3_02145"
     CDS             5968..6591
                     /locus_tag="ACZPD3_02145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR1414839.1"
                     /translation="MTYIENVFLCMASPLLIAALCMGKRQRKFFLFCFAGMGVCLLSA
                     YINTFFAALYKADTFAATTEIAPVVEEVMKLLPLLFYLLIFEPKAEQIKNAAVITALS
                     FATFENVCYLIQNGAGHFSFIFFRGIGTGAMHVICGAIVGGGLAYVWQRTWLEIAGTC
                     GLLGAAITFHAIYNLLIAYGGAAQYIAYLLPVLILAAGKLIFRSSIQ"
     gene            6745..7065
                     /locus_tag="ACZPD3_02150"
     CDS             6745..7065
                     /locus_tag="ACZPD3_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1414840.1"
                     /translation="MYDTEKRIELVKKRMHEYHRRQERRTVCRLSVLCTLLFLSLVGA
                     MGIMQNKPINVTGMYGTILLHEDAGGYVLVAVISFTVAVVITALCIKFRKKGQKSQDA
                     ENHV"
     gene            7087..>8734
                     /locus_tag="ACZPD3_02155"
     CDS             7087..>8734
                     /locus_tag="ACZPD3_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414841.1"
                     /translation="MRKKLLAGMLALALCSTNMPLQTIFAEEFTSGNPDVVSEEETPE
                     IFTNEEQEAVGETDEELSVFSSEEVPEFNDAPDEAMAAAENEQAGEIDLADNDKVMNG
                     VYTISSAGDYKFTCSRETGNRIVVDGKKISAKDSINIYLNKVNINTSAGPALRINLNV
                     EATVTIHLTGTNSLITKNNYYAGLQKDNKARLIIKTNNSDATAGILNARSIDGDSAGI
                     GGGFYGSVSCSNIIIDSCSVIASSKYGAGIGGSKQRAGSVSDIIINSSSVTASSTDGA
                     GIGGGGYGASSVSGITINSSSVTASSTNGAGIGGGGYGADSVSDITINSSSVTASSTN
                     GAGIGSAGGTCSNIGISGGSVKAYSDRMPGINCTPHNGNSTNVYCCIIKNEYFLPVTI
                     DSESWKPSYHIFPDSTKDGNLYVWLTEKENNDAYDVTVGTEKRQYSFDQAKNQFVRIQ
                     ITPTADQFDYTQPNFTYTKDTHVDISKYIKWKDDVTGHGKITKVTYFKKGDKTPLADS
                     PTDAGTYTFKIDVNEGDYYNSVDSISAPEWEFVISKAQAPS"
BASE COUNT         2543 a         1620 c         2192 g         2379 t
ORIGIN      
        1 gtttatcgtt ttggtgtccg tatttactat ggcgcaccga gcaaaaaaga attccagaca
       61 attgtgaaag ctctcgcaga gagaaacgga attaccatgc cggaggacga gctgcttctg
      121 gaagcaaata agtgggagct ttcccatgga ggacttacag gacgaactgc acagcagttt
      181 atcgaccatc tgctgggtgt ggagaaggaa taaaaaagca aatagatgaa tggtaaaggg
      241 ggaacgcata tggcagtcat gacatttgca gccattgaca tcggatctta tgaagtcagc
      301 atgaagatct ttgagatgtc aaaaagaatt ggttttcgtg aactgaatga tgtcagatac
      361 agtctggaaa tcggaaaggg tgtctattct gacggaaaga tcgattcaga aatgctgaat
      421 gttttatgtg aggtgctgaa cgactttaag aggctgatgc aggactttgg ggtggaagag
      481 tatcgtgcct gcggcaccag tgcattcaga gaacttgtga atccgctgct gatcattgaa
      541 cagatctatc agcgtacagg aatgaaaatt gaaatattaa gcagtgcaga acagcatttc
      601 cttgggtaca aatcaattgc tgctatagaa aagggattca aaaagatgat ccagaaaggc
      661 accgcgatcc tggatgtggg cggcggaagc cttcaggttt ccctctttga caaggatgct
      721 ctggtcacta cccagggact taaaatggga agtttaagaa tccgccagcg tctgcaggaa
      781 ctggagaaaa caaccattca ttatgacaag ctggttgagg aatttatccg aaatgacctg
      841 atgagctttc agcgtctgta tctgaaggat aaggagatca ggaatgtgat cctgatggga
      901 gattttatta cagatatgat cttccaggag gaaatggaag acaggatcat tacaagggaa
      961 gaatttatga aacgctatga ggatacagtt ggaaaatctg ttgacctgct ggcacaggag
     1021 atggagattg atccggaata tgcatccctt gttgtgccga ccatggtagt gtgcagaaat
     1081 tttattgata ttttcaatgc agagtctctc tgggcaccag gtgtttccct gctggatgga
     1141 attgcctatg attttgcaga gaagaaaaaa tttctcaaaa gtgtccacaa ttttgagaat
     1201 gacattctgg ttacatcaaa gaacattgcg aaacgctatt ccagcagtaa gagccatatc
     1261 cagggaacta tgaatctttg cctgaatatc tttgacagta tgaagaaggt tcatggtatg
     1321 ggagcaagag aacgtctgtt attgcagatc gcggcactgc ttcatgactg tggcaaatat
     1381 atcagtatgg aaaaggtttc cgagtgttcc tatcagatca ttatgtctac agatatcatt
     1441 gggctgtcct ttatggaacg ccagatgatc gcatgtgcgg tcagattcaa taattctgcg
     1501 tttgtttatt atgatgaact ttacagtatg ggaatcgcta ttgacagaga gagctatatc
     1561 aaggtggcaa agatgactgc aatccttcgc ctggcaaatg ccatggacag aagtcattat
     1621 cagaaagtaa aaggaatcaa agcagtgctc aaagacagag aactgcagat gatcgtggat
     1681 tctgcacagg acatttccct ggaactggga ctgctgaaag ataaagaggc attttttgaa
     1741 gaagtatttg gcattcgtct ggttctcaga cgcaagggca gagcgtaaag gaggatgaac
     1801 ttatggactt tgaaaaattt gaaaagaaag aatatttcgt gaatcgcgag ctgagctggc
     1861 tcaaatttga tgagcgtgtt ctgagtgaag caagagataa aaatcttcct ttgtttgaca
     1921 gactgaagtt cttaagcatt accgcatcca atctggatga attctttatg gtgcgtgtgg
     1981 cgtctcttaa ggatcaggta catgcaggat atcataaaac agacattgca ggaatgacag
     2041 ccaaggagca gttaaaagaa atcagtgtcc gtacccatga actggttcat gtacagtata
     2101 atacactgaa ccgttctctg atccctgcgc ttgagaaagc cggaatgcac ctggtggcag
     2161 cccatgagaa tcttacagaa gcacagagtg tatttgtaga cagatacttt gaggataatg
     2221 tatatccggt acttacaccg atggcgatgg attcttccag accgtttccg ctgatcagaa
     2281 acaagacatt aaatatcggt gctctgatct ctaagaagga gaagagtgat aaactcagca
     2341 aaaaggataa aacaggagaa cttctgtttg ctacagtgca ggttccgtct gtgcttccaa
     2401 gggtggtgca gattccgtca aagaaggatg gagataccac agtgatcctg ctggaagaga
     2461 ttattgaaag aaatattgac aaacttttcc tgagttatga tgtgatatgt gcccatcctt
     2521 accgtattat gcgtaatgcg gatcttacca tcgatgaaga tgaggcagag gatctgctgg
     2581 tggagatcca gcgccagtta aagaaacgtc agtggggtga ggttatccgt ctggaagtag
     2641 aggataagat ggatgaacgc ctgctgaaga ttctgaaaac ggaatttgat atcaaagagg
     2701 cagatatttt tgagataaac ggacctctgg atcttaccat gcttatgaaa gtatatggtg
     2761 cggatggctt tgatgcttac aagacaccga gatatcagcc ggcacctgta ccggaatttc
     2821 agaatgagaa ggatattttc caggtgatac gtgagggcga tgtattcctg catcatcctt
     2881 atatgagctt cgatcctgtt gtaaatttcg tacgtcaggc agcaaaagat ccggatgttc
     2941 tggctattaa acagactctg tatcgtgtca gcggcaattc accgatcatt gctgcccttg
     3001 cgcaggcagc agaaaacggc aaacaggttt ccgtgctggt agagctgaaa gcacgttttg
     3061 atgaagaaaa taatattgta tgggcgaaga agctggagaa ggcaggatgt catgtaattt
     3121 atggtctggt aggccttaag acacacagca agatcacact ggttgtgcgc agggaagaga
     3181 caggaatccg ccgttatgtg catctggcaa ccggaaacta caatgattcc actgccaaac
     3241 tctatacaga ctgcggtatc tttacctgcg atgaacgttt tggtgaagat gcaacagcag
     3301 tatttaacat gctctctgga tattctgaac cgaaaagctg gaataagctg atcgtagcac
     3361 caatctggat gaaggacagg ttcttaagcc tgattgaacg tgaggcagag aatgcgaaga
     3421 aaggacttcc ggcgttaatc cgcgcaaaga tgaattctct ctgtgatccg aagattattg
     3481 ctggattgta ttatgcatct tcctgcggag ttcaggtgga gcttctggta aggggtatct
     3541 gctgtctgaa agtaggagtg ccgggaatca gtgagaatat tcatgtccgt tctattgtag
     3601 gggaatttct ggagcatagt cgtattttct attttgaaaa cggcggaaac ccggaaatct
     3661 atatgggaag tgcggattgg atgccgcgta accttgaccg cagggtagag attgttttcc
     3721 ctgtggaaga tgagaagatt aagaaagagc tggagcatgt acttgatctg gagtttaagg
     3781 ataatgtaaa agctcatatc ctgcagccgg atggaactta tgtgaagccg gataaacgag
     3841 gcaaagccca gattaactct cagatggaat tctgtattga ggcaactgag aaagcagcac
     3901 ttcataaaca cgaagaaaag aaatcccgcg tattcattcc agcagagcct gcggaggata
     3961 tagaggaata agaaacagtc agaaacagaa ataaataact gggaaatgcc atggaaagcg
     4021 gtggagctgc catggcattt cttctctggg acaaaaaaat aaaaattttt ttgtggcaaa
     4081 aaccgcataa atacagggct ttttgaacga aaaattcgag aaaaataaaa aaattgaaat
     4141 ttttttaaaa aagtacttgc attttagaaa aagatggtgt atattaaacg agtcgacagc
     4201 gaagagcaaa cgacagataa acaaatggcc agttggtcaa ggggttaaga cgtcgccctc
     4261 tcacggcgaa aacacgggtt cgattcccgt actggctgct acaaaatagt gagaatttaa
     4321 ccagagctta ggctctggtt atttttttat cagggatttc tcctgacatg ttatatgtct
     4381 tcgcagacaa aaatgattca cgaacctgga gctttcatct gacaatagag gagtaatact
     4441 tggaaaggaa gaacgatatg gtcactgatg aagaacttta tgggcaatat ctgtgcggtg
     4501 atgagacagg acttgaactg ttgataaaaa aatacggtga cccgctgacc ttatatattg
     4561 atggttatct gcatgatgtc cacgaagcgg aggatttaat gatggaaacc ttttcctggc
     4621 tgtttatgaa aaagccccgc atccggaatg gatgttttaa ggcttacctc tataaagcag
     4681 cgcgtcacat ggcattacgc cacaaaagca gacggcggat tatcttcagt cttgacaacc
     4741 tgaccagaga agcggaggca caaacgctgg tagaggaggt cgtccgcaca aaagagcgaa
     4801 accagatcct gcatctctgc atggacgagt taaatccaga ttatagggag gctctttatc
     4861 tgacctattt tgagggcatg agctatcaac aggccgctga ggttatgggg aaaagtgtaa
     4921 agcagattac aaatatggtg taccggggga aagagcgttt acgcggatta ttgaaacggg
     4981 agggtattac aaatgctgaa agatgaggaa cggattgcgg aggtaaaacg gcgtattgca
     5041 aaaaaagaac aacagcagag actgcggcgc agacggatta tctctgctgt ctgtatagcg
     5101 gcatgttttg cagtaatcgt tggggtttcc tttgccatgc ccggtattgt cggacagatc
     5161 gaacccggta cttcttcagg ctttgaaact gcggcaacaa ttttgggcgg cagcaccgca
     5221 ttgggatata tagtgatcgg actgttggct tttgttctcg gcgcgtgcgt gactattctg
     5281 tgttttcgca tccatcagct gaacaaagag gagcagacgg agaaacaaaa ggaggacaat
     5341 ggggatggag ctgatcagta acctttttca attcgctgtt accctgttgg gcttttgtat
     5401 gagtggtatt cggtatctaa aaggtcgaaa acagacatat ttcctgctga cctgctttta
     5461 cggctgcttt gcccttggct ctctttactg gacgctgtat ctgcttttgt tttctgaaac
     5521 gccgcaggtc ttttatgtgt cagagttcgg ctggatagct agcgtgattt tcctttatct
     5581 tctgcaatac accctttcct ctgcagagga aagagacttt tcgacacgga aatctctgat
     5641 agctcctctg atcggcgttc cgctttgtgt attttactgt acttttgggg atgttctctc
     5701 caatctgctc tggtgtggca tgatgatcgt tgtttcttat cattctatcc ggggtcttgc
     5761 ttatgcgcag atacagacgg gaacagcgtg taagatgcgg tatttccaca ttggggtact
     5821 gtgctatgtg gcggtggagt atgtcctttg gatctccggc tgcctgtggc cgggctattc
     5881 catttccgct ccctattgct ggcttgatct tctgcttaca ggctgcctgt ttgcattgct
     5941 gccagccaca ggaaaggcgg tgcaggtatg acatatattg aaaacgtatt tctttgcatg
     6001 gcctcgcctc tgctgatcgc cgctctgtgt atgggaaagc ggcagagaaa attctttctg
     6061 ttctgctttg ccgggatggg tgtctgtcta ctttccgcat acatcaacac ctttttcgct
     6121 gcactttata aagcggacac ctttgcagct accacagaga tcgccccggt ggtggaggaa
     6181 gtgatgaaac ttctgccatt gctcttttat ctgctgatat ttgagccgaa agcggagcag
     6241 atcaaaaatg ctgccgtgat cacagccctt tcctttgcca catttgagaa cgtctgctat
     6301 ctgatccaaa acggagccgg tcatttttct tttattttct tccggggtat tgggactgga
     6361 gccatgcatg tgatctgtgg agccattgtc ggcggtggcc ttgcatatgt gtggcagaga
     6421 acatggctgg agattgccgg aacctgtggt ctgctgggag cagcaatcac ctttcacgca
     6481 atttataatc ttctgatcgc ctatggcggt gcagcacagt atattgctta tctgctgcct
     6541 gtactgatcc tggcagcggg aaaactgatc ttccgcagtt cgatacaata atataacctg
     6601 atttttcgct tttgggagct gtttcggcag ctttcaaatt tttttgaaaa aatttgattt
     6661 tcaggtgaga gtttttcgct ttttttgtac ctatataagt gaaaggactt ttaagactgt
     6721 ttttgtggaa ggaggttgga aggcatgtac gatacagaaa agcgtatcga attggtaaaa
     6781 aagcgaatgc atgaatacca ccgccgccag gagcggcgca cagtctgtcg gctgtctgtt
     6841 ctgtgtaccc tgttattcct gtctcttgtg ggagcaatgg ggataatgca aaacaagcca
     6901 ataaacgtta cgggaatgta cggcacaatc ctgcttcatg aggacgcagg cggttatgtg
     6961 ttggtggctg tcatttcctt tacggtggca gtcgtgatta ccgcattatg tatcaaattc
     7021 agaaaaaaag gacaaaagag tcaggatgca gaaaatcatg tataaagaaa ggaaaaggtg
     7081 aaaagtatga ggaaaaaatt attagccggg atgctggcac ttgcactgtg ttctacaaat
     7141 atgccccttc agaccatatt tgcggaggaa tttacttccg gaaatccaga tgtagtgtct
     7201 gaggaagaaa ctccggagat ttttaccaat gaagagcagg aagctgtagg agagacagat
     7261 gaggaactgt ctgtattcag ttcagaagag gttcctgaat ttaatgatgc ccccgatgag
     7321 gcaatggctg ctgcggaaaa tgaacaagct ggagaaattg atttagctga taacgataag
     7381 gttatgaacg gagtttatac tataagttcg gcaggagatt acaaattcac atgtagccgg
     7441 gaaacaggaa atagaattgt ggttgatgga aagaaaattt cagcgaaaga cagcatcaac
     7501 atttatctta ataaagtaaa tatcaataca tctgctggtc cggcactgcg aataaatctt
     7561 aatgttgaag ctacagtcac tatccattta acaggcacaa atagtcttat aacaaagaat
     7621 aactattatg ccggtttaca gaaagataac aaagctcggc ttataataaa aaccaataat
     7681 tcagatgcta ctgcaggaat cttaaatgct cgtagtatcg atggggatag cgccggaatt
     7741 ggtggcggct tttacggctc cgtctcttgc agtaatatta ttattgatag ttgttctgta
     7801 attgctagtt ctaagtatgg tgctggaatt ggtggcagca agcaacgcgc tggcagtgtc
     7861 agtgatatta tcattaatag tagttctgta actgctagtt ctacggatgg tgccggaatt
     7921 ggtggcggcg gttacggcgc tagcagtgtc agtggtatta ccattaatag tagttctgta
     7981 actgctagtt ctacgaatgg tgccggaatt ggtggcggcg gttacggcgc tgacagtgtc
     8041 agtgatatta ccattaatag tagttctgta actgctagtt ctacgaatgg tgccggaatt
     8101 ggttctgccg gtggcacctg cagcaacatt ggcattagtg gtggatccgt aaaggcatat
     8161 tctgatcgta tgccaggcat taattgtacc ccccataatg gcaactcaac aaatgtttat
     8221 tgttgtatta ttaaaaatga atatttttta ccagtaacga tagatagtga atcctggaag
     8281 ccaagttatc atatttttcc tgattctaca aaagacggta atctgtacgt ctggttaact
     8341 gaaaaagaaa ataatgacgc ttatgatgta actgttggaa ccgaaaaaag gcagtattct
     8401 ttcgatcagg caaaaaacca atttgtacgt atccaaataa ctcctaccgc tgaccaattt
     8461 gattatacgc agcccaattt tacatatact aaagataccc atgttgatat tagtaaatat
     8521 attaaatgga aagatgatgt caccggtcat ggtaaaatta ctaaagtaac gtattttaaa
     8581 aaaggagata aaactccact cgcagacagc cccacagatg caggtactta tacttttaag
     8641 attgatgtga atgaaggtga ctactacaat tcagtcgata gtatatctgc accagaatgg
     8701 gaatttgtca tttcaaaagc acaagcacct tcaa
//
LOCUS       DBMDXG010000037         8548 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2215_length_8548_cov_1.856162, whole genome shotgun sequence.
ACCESSION   DBMDXG010000037 DBMDXG010000000
VERSION     DBMDXG010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2215_length_8548_cov_1.856162"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(54..2237)
                     /gene="pknB"
                     /locus_tag="ACZPD3_02160"
     CDS             complement(54..2237)
                     /gene="pknB"
                     /locus_tag="ACZPD3_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR1414842.1"
                     /translation="MLKTGMIIAERYEILGKIGTGGMADVYKAKDHKLNRFVAVKVLK
                     PEFREDTTFIRKFKSEAQAAAVLTHPNIVNVFDVGDDNGVYYIVMELIEGITLKEYIS
                     KKGKLSVKEATSIAIQVSMGLEAAHSHGIVHRDVKPQNIIISMDGKVKVTDFGIARAA
                     SSNTISSNVMGSVHYSSPEQVRGGYSDEKSDIYSLGITMYEMVTGKVPFDGDTTVAIA
                     IKHLQEEIVPPSIYTPELPHSLEQIILKCTQKSVDRRYQNMEDVIADLKHSLIDPQGD
                     FVTLTSVDNEAKTVVISDKELGEIKHMPKQIAKSEPEALEEEINETDYDDEPEVKKHG
                     KKSDKPEKKKKRGGHGLTIVMLLMGVVILIAIILVAGKASGLIGSNNDTDKKTEASDT
                     SESDDDGMITVPNLVGKTEDEAKNITKDMKLGIQPMGEEASNQAKGTISSQDIPKGSK
                     VEQYTTIKYYISKGAQKITIPDVDGQTGVDAQQTLEDMGLTVEIQKEYSELNDDGTPV
                     TDPGYAVSTTPAAGNSVSAGDSVTLIVSRGVDYGDSVEVPSVVGMTKNDAVTTFGKFL
                     NVEVKEEKSTEVAEGEVISQEPEAGNWEDPDNVNVVITVSTGDQEPSAQSDSADTAAS
                     SDAPAQTADNSAAAAAGEVWKCTQTLNTPSGYSGGPVRLELIQNVNGTPTASVVLEDQ
                     VIQFPYDLDITGAPGISEGTLYLSEQISGTYQELGNYSITFAKAE"
     gene            complement(2231..2977)
                     /locus_tag="ACZPD3_02165"
     CDS             complement(2231..2977)
                     /locus_tag="ACZPD3_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR1414843.1"
                     /translation="MRIYSATDVGQKRKMNQDYVFATADPVGNLPNLFVVADGMGGHN
                     AGDYASSHAVTSMVEEIRQDADFNPVKVIRHAIECVNTEILAQAQQDEKLRGMGTTIV
                     AATIVGHYAYVANVGDSRLYVIGEKIQQITRDHSLVQEMVRMGELDPEQARKHPKKNI
                     ITRALGAEKTVDIDFFDLKLEPGDVVLMCSDGLSNMVEDSQLREIISDTSTDLDEKGR
                     ILIREANRNGGKDNIAIVLVEPFTDEVKAC"
     gene            complement(2979..4019)
                     /gene="rlmN"
                     /locus_tag="ACZPD3_02170"
     CDS             complement(2979..4019)
                     /gene="rlmN"
                     /locus_tag="ACZPD3_02170"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR1414844.1"
                     /translation="MTDIKSMTIDELKKLMTTLGDKPFRAKQIYSWLHEHLVTSYDEM
                     TNLSKSLREKLKEYPVTALKMVDVQTSRIDGTQKYLFRLSDGNVIESVLMRYKHGNSV
                     CISSQVGCRMGCRFCASTIGGLTRCLLPSEMLDQIYRIQALTGERVSNVVVMGTGEPL
                     DNYENLLRFIHILTEEGGLHISQRNLTVSTCGLVPKIYDLAKEKLQMTLALSLHAPND
                     VKRRELMPIANKYSMDEVLEACRYYFKETGRRITFEYSLVAGVNDSDEDARELSGRIR
                     DMNCHVNLIPVNPIKERSFVRSTRQAVENFKIKLEKCGINVTIRREMGSDIDGACGQL
                     RKSYMEKTESEK"
     gene            complement(4023..5375)
                     /gene="rsmB"
                     /locus_tag="ACZPD3_02175"
     CDS             complement(4023..5375)
                     /gene="rsmB"
                     /locus_tag="ACZPD3_02175"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR1414845.1"
                     /translation="MTNGINTRELILQILLEIEEEGKHSHIAIRNALSKYQFLPRQER
                     AFITRVCEGTLEYRILIDYIIDSYSKVSVDKMKPVIREILRSAVYQIRFMDSVPDSAV
                     CNEAVKLAQRKGFYSLKPFVNGVLRTIAREWKNLKLPSREENPVRYLSVRYSMPETLV
                     NRWLEDYGEEKTEKILTDFLTEKPITVRCRTHKYPQKEIYESLVDQGVEVKPAPYLPY
                     AYEISNYNHILALDAFIQGKIQVQDVSSMLVAEIADPQKGDYVIDMCAAPGGKSLHVA
                     DKMGDYGTVDARDISQYKVNMIEENIHRTDCINVQAHVMDATVFDVDSELKADVVLAD
                     VPCSGYGVIGKKPEIKYRVTAQKQEEIVILQRTILDNAAEYVKPGGVLVFSTCTIAKE
                     ENEENMLWFMNNHPFKLESIDPYLPEELHSETTALGYLQLLPGVHKTDGFFIAKFRRK
                     "
     gene            complement(5368..6063)
                     /locus_tag="ACZPD3_02180"
     CDS             complement(5368..6063)
                     /locus_tag="ACZPD3_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR1414846.1"
                     /translation="MYGYYYFDPTYLLLIIGMVLSLLASAKVKSTFSVYSRVRSASGL
                     TGADAARRILRMSGITDVTVVPISGSLTDHYDPGSKKLALSQDVYDKTSVAAIGVAAH
                     ECGHAIQHATNYVPLNLRSAIVPVANIGSTLSWPLFLAGLIFSIRPLLTVGIILFTFA
                     VLFQLVTLPVEFNASSRALKMLGSSGMLGNDEVKGARKVLTAAALTYVAALASSILQL
                     LRLIILAGGRDRD"
     gene            complement(6068..7015)
                     /gene="fmt"
                     /locus_tag="ACZPD3_02185"
     CDS             complement(6068..7015)
                     /gene="fmt"
                     /locus_tag="ACZPD3_02185"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR1414847.1"
                     /translation="MKIVFMGTPDFAVPSLHALTEAGYAVAAVVTQPDKPKGRGKTLL
                     PTPVKEEAVMHDIPVYQPEKVRNNPEFLEILKEINPEIIVVAAYGQIIPKEILELPKF
                     GCINIHASLLPKYRGAAPIQQAVIDGEKVSGVAIQQMGEGLDTGDMISKIVIPISPTE
                     TGGSLFGKLAQAGADLLIKTLPSIEQGTAEFEKQPEESPTPYAAMITKQMGLMDFRKS
                     AEELERLVRGLNPWPSAYTFLNGKTLKVWKCKVSTEKTDAVPGTIFLADKEGIHTACG
                     EGVLILTEVQLEGKKRMETEAFLRGYHIENGIVFTDHKE"
     gene            complement(7023..7499)
                     /gene="def"
                     /locus_tag="ACZPD3_02190"
     CDS             complement(7023..7499)
                     /gene="def"
                     /locus_tag="ACZPD3_02190"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1414848.1"
                     /translation="MALRKIRLQGDEVLAKVSRPVEKMTPRIHDLIGDMLETMYDAMG
                     VGLAAPQVGMLKRIVVIDIGEGPIVLINPEILETSGEQTGDEGCLSVPGMAGQVTRPN
                     YVKVKALDEDMNEVEYEGEGLLARAFCHEIDHLDGHMYTELVEGELHQVSYDEDEE"
     gene            complement(7669..>8548)
                     /gene="priA"
                     /locus_tag="ACZPD3_02195"
     CDS             complement(7669..>8548)
                     /gene="priA"
                     /locus_tag="ACZPD3_02195"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1414849.1"
                     /translation="SCRSCGEVLKCPHCDVALAEHNNGKMICHYCGYQRPKAEVCPSC
                     GSPYIGGFKAGTQQIEAVLHKCFPKAGILRMDYDTTRTKGSYEQILSSFAAHEADILV
                     GTQMIVKGHDFPDVTLVGAIAADLSLNASDYQCAERTFQLLTQAVGRSGRGKKPGEAV
                     IQTYHPDHYSIQAAAVQDYQAFYEEEMSYRMLLDYPPAAHMLAVLGSGPEDEKLVQAM
                     YYLKLYIERIYRENDLHIIGPAYASVGKVKDIYRQVIYLKHKDHKTLVHIKDQLEKYI
                     EINSGFRKIYIQFDFS"
BASE COUNT         2117 a         2184 c         1556 g         2691 t
ORIGIN      
        1 gcatagatgt aataaaatcc tgcaattcct tttattatct ttccctgcat ggatcattca
       61 gccttagcaa atgtaataga ataatttccg agttcctgat aagttccact gatctgttca
      121 gacagataca aagtaccctc gctgatacca ggtgcacctg tgatatccag atcatatggg
      181 aactggatta cctgatcttc cagaacaact gaagctgtcg gtgttccgtt tacattctgg
      241 atcagttcca gacgaaccgg acctccggaa tatcctgacg gagtattcag agtctgtgta
      301 catttccata cttccccggc tgctgccgcc gcactgttat ctgctgtctg tgccggagca
      361 tcagatgacg cagcagtatc tgcgctgtca ctctgagccg aaggttcctg atctccggta
      421 cttactgtga tcacaacatt cacattatcc ggatcttccc agtttccggc ttccggttcc
      481 tgtgaaatta cttctccttc tgccacctct gtactctttt cctcttttac ttccacattg
      541 aggaacttac caaaagtagt aactgcatca ttctttgtca ttccgacaac acttggaacc
      601 tccacactgt ctccatagtc cacgcctcga ctgacaatca gtgttacgct gtcccctgct
      661 gaaacagagt ttccggctgc cggagttgtg gagactgcat atcccggatc tgtaaccgga
      721 gtaccatcat catttaattc actgtattct ttctgtatct caacagttaa tcccatatct
      781 tccagagtct gctgtgcgtc tactccggtc tgtccatcca catccggaat tgtgatcttc
      841 tgcgcaccct tgctgatata atacttaatg gtcgtatact gctctacttt acttcccttt
      901 ggaatatcct gtgaagaaat ggttcctttt gcctgatttg aagcctcctc gcccataggc
      961 tgaataccca gtttcatatc ttttgtgata ttctttgctt catcctctgt ttttcctacc
     1021 agattcggca cagttatcat tccgtcatca tctgattccg atgtatctga ggcctcagtt
     1081 tttttgtctg tatcattgtt acttccaata agtccggacg cctttcctgc gaccagaatg
     1141 attgctatca gaatcacaac acccatcaga agcattacaa tagtcagccc atgtccgcct
     1201 ctttttttct ttttctcagg cttatctgat ttcttcccgt gctttttcac ttccggctca
     1261 tcatcataat ctgtttcatt gatttcttct tccagagcct caggttctga ctttgcgatc
     1321 tgcttcggca tatgtttgat ctcgccaagc tccttatctg agatcacaac tgtttttgct
     1381 tcgttatcca cagacgtcag cgttacgaaa tctccctgtg gatcgatcag agaatgcttc
     1441 agatctgcga tcacatcctc catattctga taacggcgat ccacgctttt ctgtgtacat
     1501 ttcagaatga tctgctccag actgtgggga agctccggtg tataaatact tggaggaaca
     1561 atctcttcct gaagatgttt gatagcaata gctactgtgg tatctccgtc aaaaggaacc
     1621 ttccctgtaa ccatttcata cattgtgata cccagtgaat aaatatcact cttctcatca
     1681 ctgtatccgc ctctgacctg ttccggtgaa ctgtaatgaa cagatcccat tacatttgag
     1741 cttatggtat tggaagaagc ggctcttgca ataccaaaat ctgttacttt tacctttcca
     1801 tccatggaaa tgataatatt ctgcggtttt acatcacgat gtacaatccc atggctgtgt
     1861 gcagcttcca gtcccatgga gacctggatt gcaatactgg ttgcttcttt tacagaaagc
     1921 tttccctttt tggaaatgta ctccttgaga gtaattccct caataagctc cataacaatg
     1981 taataaactc cgttatcgtc accaacatca aatacattca ctatattcgg atgtgtcagg
     2041 actgctgcag cctgagcctc acttttaaac ttgcggataa acgtcgtatc ctctctgaat
     2101 tccggcttaa gaacttttac agcaacaaac cgattcagct tatggtcttt tgccttgtat
     2161 acgtccgcca ttccgccggt accaattttt cctaatatct catagcgttc tgctatgatc
     2221 attcccgttt ttaacatgct ttcacctcgt ctgtgaatgg ttctaccaat acaattgcaa
     2281 tattatcctt gccgccattg cggtttgctt ctcttataag tatcctgcct ttttcatcca
     2341 gatctgtaga tgtgtcagaa atgatttccc tcagctggct gtcctcaacc atgttgctga
     2401 gcccgtccga acacatcaaa actacatctc cgggttccag tttcagatca aagaaatcaa
     2461 tatccactgt tttctctgct ccaagtgctc ttgtaatgat atttttcttc ggatgcttcc
     2521 ttgcctgttc agggtcaagt tctcccattc ttaccatctc ctgcaccagt gagtgatccc
     2581 ttgttatctg ctgaatcttc tctccgatca cataaagtct gctgtcacct acatttgcca
     2641 cataagcgta atgtcctacg atagtagctg ccactatcgt tgttcccatt cctctgagtt
     2701 tctcatcctg ctgtgcctgg gcaagaatct cggtatttac acattcaatg gcatgacgga
     2761 ttacctttac cggattaaaa tccgcatcct gccggatttc ctccaccata gatgtaaccg
     2821 catgagagga tgcataatca cctgcattat gaccacccat accatctgca acaacaaaca
     2881 gattcggaag atttccaacc gggtcagcag ttgcgaacac ataatcctgg ttcatcttcc
     2941 gcttttgccc gacatcagta gctgaatata tcctcatgtc acttctcgct ttctgttttt
     3001 tccatataac tttttcttaa ctgtccacag gcaccatcaa tatcactgcc catttctctt
     3061 ctaatagtaa catttattcc acatttttca agttttattt tgaaattctc cacagcctgt
     3121 cttgttgaac gcacaaagga acgttcctta atagggttta ccgggatcag atttacatgg
     3181 cagttcatat ctctgattct gccggaaagt tcccttgcat cctcatcgct gtcattaact
     3241 ccggctacca gactgtattc aaatgtgatc ctgcgcccgg tttctttaaa ataataacgg
     3301 caagcttcca gaacctcatc catgctgtat ttgtttgcga tcggcatcag ttcccgtcgc
     3361 ttcacatcat taggtgcgtg aagggaaagt gcaagtgtca tctgaagctt ttcttttgca
     3421 aggtcataaa tcttcggaac caacccacag gtagatacag tcaggtttct ctgactgata
     3481 tgaagtcctc cttcttctgt caggatatgt ataaacctga gaagattttc ataattatca
     3541 agaggttccc ccgttcccat gaccacaaca ttggaaactc tttctcctgt cagagcctgt
     3601 atcctgtata tctgatccag catttctgac gggagaagac atctggtcag tcctcctatg
     3661 gtagatgcac agaatctgca tcccatgcgg catcccacct gagaagaaat acagacagaa
     3721 tttccatgtt tataccgcat gagaacactc tctatcacat ttccatcact gagacggaac
     3781 agatacttct gtgtaccgtc aatccgggat gtctgtacat ctaccatctt cagtgctgtg
     3841 acaggatatt ctttcagttt ttccctcagg cttttggaaa gatttgtcat ctcatcataa
     3901 gaagtaacaa ggtgttcatg cagccagctg taaatctgct tcgcacgaaa aggcttatct
     3961 cccagagtgg tcatcagttt ctttaattcg tcgatcgtca ttgacttaat gtctgtcatt
     4021 acttattttc tcctgaattt tgcaataaag aagccatctg ttttgtgtac tcccggcaga
     4081 agctgaagat aacccagagc tgttgtctca gaatgtagtt cttccggaag atacggatca
     4141 atactctcca gtttgaacgg atgattattc ataaaccaca acatgttctc ttcattctct
     4201 tcctttgcga tagtacaggt actaaatacc agcactccgc ccggtttcac atattctgcc
     4261 gcattgtcaa ggattgttct ctgaaggatt acaatctctt cctgcttctg tgctgtcacc
     4321 ctgtatttga tctccggctt ctttccaatt actccatatc cggaacatgg gacatctgcc
     4381 agaacgacat ctgccttgag ttctgaatct acatcaaata ccgtagcatc catcacatga
     4441 gcctgcacat taatgcagtc tgttctgtga atattctctt cgatcatatt cactttgtac
     4501 tggctgatat cgcgtgcatc tactgttccg taatctccca ttttatctgc aacatggaga
     4561 ctctttccac caggtgctgc gcacatatct atcacataat cccctttttg cggatctgca
     4621 atttctgcca ccagcattga gcttacatcc tgcacctgga ttttcccctg tataaatgca
     4681 tccagtgcaa gaatatggtt ataatttgaa atttcatatg catagggaag ataaggtgca
     4741 ggtttcactt ccactccctg atcgaccaag ctctcataaa tctctttctg tggatattta
     4801 tgagttctgc agcgtactgt aatcggtttc tcagtgagaa aatctgtaag gatcttttct
     4861 gttttttctt ccccgtaatc ctcaagccaa cggtttacca gtgtttccgg catagaatat
     4921 ctgacagaca gatatctgac cggattttct tcccttgaag gaagtttcag atttttccac
     4981 tctctggcaa tcgtacgaag aacaccgttt acaaaaggtt tcaggctgta aaagcctttt
     5041 ctctgtgcca gcttcaccgc ttcattacac actgcgctgt ccggcacact gtccataaac
     5101 ctgatctgat aaactgcact tctcaggatt tccctgatca caggcttcat tttatctaca
     5161 gatacttttg aatatgaatc aataatataa tctattaaaa tacgatattc cagtgttcct
     5221 tcgcagactc tggtaataaa agctctctcc tgtcttggaa ggaactgata tttggaaaga
     5281 gcattgcgga tagcgatatg gctgtgtttt ccctcttcct cgatttccag taatatctgt
     5341 aatatcagtt ctcttgtatt tattccatta gtcacggtct cttcctccgg caagtatgat
     5401 cagtcttaaa agctgcagga ttgatgaagc aagtgctgca acataggtga gtgctgccgc
     5461 tgtaagaacc ttccttgcac cttttacctc atcgtttcca agcattcctg aacttccaag
     5521 cattttcaac gctcttgaag aagcattgaa ttctaccgga agagttacaa gctgaaaaag
     5581 aaccgcaaat gtaaacaaaa tgattccaac agtcagcagt ggacggattg agaaaatcag
     5641 tcctgccaga aacaatggcc aggaaagtgt tgaaccgatg tttgcaacag gcacaattgc
     5701 agagcgaaga ttcaatggca catagttagt cgcatgctga atagcatgtc cacattcatg
     5761 cgcagccacc ccgattgcag ccacagatgt tttatcatat acatcctggg aaagtgccag
     5821 tttcttactt cccggatcat agtggtctgt caggctgccg gatatgggta ctaccgtaac
     5881 atccgtaatt cccgacatac gaagaatccg tctggcagca tcggcgcctg taagccccga
     5941 tgcactgcgc actctgctgt aaacagaaaa cgtactcttt acctttgcag atgccagtaa
     6001 agacagcacc attccgataa tgagcagtaa atacgtagga tcaaaataat aatatccgta
     6061 catctaatca ctccttatga tctgtaaaaa caattccatt ttcaatgtga tatccccgga
     6121 gaaaggcttc tgtttccata cgcttcttac cttccagctg tacctctgta agaatcagca
     6181 ctccttctcc acatgctgta tggatcccct ctttatctgc caggaagatt gttcccggaa
     6241 cagcatctgt tttctcagtg ctgactttac atttccacac tttcagagtt tttccattaa
     6301 gaaatgtata agcacttggc catggattca ggccgcgtac aaggcgttcc agttcttctg
     6361 cagattttct gaaatccatc agtcccatct gtttggtgat catagccgca taaggtgtag
     6421 gactctcctc cggctgtttt tcaaactccg cagttccctg ctcaatagat ggaagtgtct
     6481 tgatcagcag atccgcaccg gcctgcgcaa gttttccaaa caggcttcca cctgtttctg
     6541 tcggagagat cggaattact atctttgaaa tcatatcacc ggtatcaaga ccttctccca
     6601 tctgctggat cgctacacct gatacttttt ccccgtcgat cacagcctgc tggataggcg
     6661 ctgctcctct gtatttcgga agaagagacg catgaatatt gatgcatcca aatttcggaa
     6721 gctccaggat ttcttttgga ataatctgtc catatgcagc aacaactatg atctcaggat
     6781 tgatttcttt cagaatctcc agaaattcag ggttatttct cactttctcc ggctgatata
     6841 ccggaatatc gtgcataacg gcttcttctt ttacaggtgt tggcaataat gtcttacctc
     6901 tgccttttgg tttgtcaggc tgggtcacta ctgctgccac cgcatatccg gcttctgtca
     6961 gtgcatgtaa tgacggcact gcgaaatccg gtgttcccat aaatactatc ttcatttgat
     7021 tatcactcct catcctcatc ataggaaacc tgatgaagtt ctccttctac cagttctgta
     7081 tacatatgtc cgtccagatg atcgatctca tggcagaaag cacgcgcaag aagaccttcc
     7141 ccttcatatt ctacttcatt catatcctca tccagagctt ttacctttac atagttcgga
     7201 cgtgttacct gaccagccat tccaggtaca cttaagcatc cttcatcacc agtctgttcg
     7261 cctgatgttt caaggatttc aggattgatc agcacgatag gaccctctcc tatatcgatc
     7321 acaacaatac gcttcagcat acctacctgt ggtgcagcga ggccaactcc cattgcatca
     7381 tacatggttt caagcatatc cccgatcaga tcatgaatac gtggtgtcat tttttctacc
     7441 ggtctgctga cctttgcaag tacttcgtcg ccctgtaatc tgatttttct aagtgccatt
     7501 tctttattct cctcttcttt gattatgatg ttagaatcaa aagatatttt gcccctaacc
     7561 tgatatcttg attattgtta tatcacgatt tcagaaatta ctgccttccg gatttccctt
     7621 ctgcagcaga ttacttcagt aatgagattt tgaaaaaatc tgccataatt aactgaaatc
     7681 aaattgtatg tatattttcc ggaaaccgga atttatctct atgtatttct ccagctggtc
     7741 tttgatatgt accagcgttt tatgatcttt atgttttaaa tagataacct gtctgtaaat
     7801 atccttaact tttccaactg acgcatatgc cgggccaatg atatgcaggt cattttctct
     7861 gtatatcctt tctatataaa gtttcagata atacattgcc tgaaccagtt tctcatcttc
     7921 cggacctgaa cccagaactg ccagcatatg tgccgcaggc ggataatcca gaagcatacg
     7981 gtaactcatc tcttcctcat agaatgcctg ataatcctgc actgccgccg cctgaatact
     8041 gtaatgatcc ggatgatagg tctgtatcac agcctctccc ggcttttttc cccttccgga
     8101 tcttcccacc gcctgagtga gaagctgaaa ggttctttct gcgcactgat aatcagaagc
     8161 attcagagaa aggtctgccg ctatggcacc taccagtgtc acatccggaa aatcatgtcc
     8221 cttgacgatc atctgagttc ccaccagaat gtccgcctca tgggctgcaa aggaagaaag
     8281 aatctgttca tagcttcctt ttgttctggt ggtatcgtaa tccatgcgca gaataccggc
     8341 ttttggaaaa catttatgaa gaacagcctc tatctgctga gttcccgcct taaatcctcc
     8401 gatatacggc gaaccgcagg agggacatac ctctgctttt ggcctctgat agccgcagta
     8461 atgacagatc atttttccat tgttatgttc agccagagcg acgtcgcagt gaggacattt
     8521 cagcacctcc ccgcaggaac ggcaggat
//
LOCUS       DBMDXG010000038         8514 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2228_length_8514_cov_2.091050, whole genome shotgun sequence.
ACCESSION   DBMDXG010000038 DBMDXG010000000
VERSION     DBMDXG010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_8514_cov_2.091050"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="ACZPD3_02200"
     CDS             complement(<1..590)
                     /locus_tag="ACZPD3_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414850.1"
                     /translation="MKNRKLNQLLVAGMISAAMMTSGISVQAADFSDDTSTVEEQSVG
                     SAEEEAPEVEDGSEFQSDAAEASSAVAVSSANFPDAKFRQYVLDNIDTDKDKKLSAAE
                     IKAAKTIDVSGLGISNLKGIERFTYATDLFAANNKLTSVNITKNTKVAYLNLSNNSLA
                     GTLNLSKCTNLRVVKYGSNKLTKVVMPSKKYLKNLD"
     gene            complement(729..1316)
                     /locus_tag="ACZPD3_02205"
     CDS             complement(729..1316)
                     /locus_tag="ACZPD3_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7575 domain-containing protein"
                     /protein_id="HIR1414851.1"
                     /translation="MGTDFFDGLVKTFSKTTKELGKTTRELGARAEQTIEAQKIRSKI
                     TGEERIIEKIKVDIGDIVYRRHSQGDGIDSELSALCQEIDQHYLKIREYKDNAANLKG
                     EKICPSCEREVDINVSFCPYCGTSCPTPEPAKNVENDTVSSDEDEDGSNEPEETPAEE
                     LQPEEPETEKTAETEPAQEQPVEETPVEKETMEEK"
     gene            complement(1372..2790)
                     /gene="clpX"
                     /locus_tag="ACZPD3_02210"
     CDS             complement(1372..2790)
                     /gene="clpX"
                     /locus_tag="ACZPD3_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1414852.1"
                     /translation="MSENYNDDEIIDSSEDTEKKRNKSEKFCFLCRRPESKAGPMIEL
                     PNNIHVCTDCMQKSFNSMNQQFNEGKFNYSDLLNMPNVSMIDLGSFQNPVQQPKKEKK
                     KKKQEKPVLDLKNIPAPHKIKETLDQYVIGQEKAKKVMSVAVYNHYKRVATDTMDEIE
                     IEKSNMLMIGPTGCGKTYLVKTLAKLLDVPLAIADATSLTEAGYIGDDIESVVSKLLA
                     AADNDVERAEHGIIFIDEIDKIAKKKNTNQRDVSGEAVQQGMLKLLEGSEVEVPVGAN
                     SKNAMVPLVTVNTRNILFICGGAFPDLENIIKERLNKQASIGFYADLKDKYDNDPHLL
                     QKVTVEDIRSFGMIPEFIGRLPIIFTLDGLNEDMLVKILQEPKNAILKQYQKLLALDE
                     VKLEFEDGALHAIAAKALERDTGARALRAILEEYMLDIMYEIPKDDSIGEVVITREYI
                     EGNGGPKILLRGQEPILLEGSH"
     gene            complement(3004..4419)
                     /locus_tag="ACZPD3_02215"
     CDS             complement(3004..4419)
                     /locus_tag="ACZPD3_02215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1414853.1"
                     /translation="MNDERNLGEKTDDSGQQPRYEHYQFHEEQRTVLKPSGPSGHRRN
                     QNSFQKKAGATIALAVIFGLVAAVVFQAANFAADRFLNTGKSSVQIKTTDSVDLQETA
                     SDDSTADKVLSDSENGTVAAVAQASMPSVVAITTVSVQEIPSFFGYSSHQYKSASTGS
                     GIIVGDNDDELLIATNNHVVDGATTLSVCFIGDDVANAETETVNAGDNGDLNVEDAVS
                     AKIKGTDADNDLAVVAVKKSDIPEDTLNQIKIAQIGSSDDLAVGQQVVAIGNALGYGQ
                     SVTSGWISALNRTISTDDGTNSTGLIQTDAAINPGNSGGALLNMKGELIGINSAKYAD
                     SAVEGMGYAIPISKAKPILEELMNRETREKVDSSKKGYLGVSLANLTTEAIEMYNMPT
                     GAFVRSVEDDSPAQEAGICKGDIIVKFDGQKVSDGDDLLDKLQYYKSGEKIEAVIARA
                     TNGEYEENTIELTLGTRPDNE"
     gene            complement(4616..5560)
                     /locus_tag="ACZPD3_02220"
     CDS             complement(4616..5560)
                     /locus_tag="ACZPD3_02220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR1414854.1"
                     /translation="MTHKEKYNDTATVDVIIPAYRPGREFGELLHRLEEQEYRPRRIL
                     VMNTGEQYWNREWEKCPILEVHHLEQKDFDHGGTRRRAAELSNADIMIFMTQDALPAD
                     RKVIGNLVCAVSENPGAGAAYARQLPRADCRFLERYTRSFNYPEQSSVKSLADIDKYG
                     IKTYFCSNVCAAYDKGIYLKTGGFTERAIFNEDMICAGTMIQKGYSVVYAADARVYHS
                     HNYSGRQQFHRNFDLGVSQAEHPEIFEGVPSEGEGIRLVKRSLGYLIRTGHFWLIPQL
                     IWQSGMKYAGYFLGKRYRKLPRKVVLACTMSPYYWNRK"
     gene            complement(5649..7067)
                     /locus_tag="ACZPD3_02225"
     CDS             complement(5649..7067)
                     /locus_tag="ACZPD3_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1414855.1"
                     /translation="MAKPGKKKKVLFVLEIIVLLLFIGGLYVYGQISSRLDKIEQPEL
                     KKEKIVVNQEAPKMTGYKTYVLFGIDTRGEGSQYSAQNSDTMIIVSVNNDTKEVRMVS
                     VYRDTLLNVGDDTYTKANAAYALGGPEQAITMLNTNLDLDISDYATADFSALVEVIDD
                     LGGLDIPLSYAEIEHMNNYCVETSKLTGKDYTPLEKPEPKPEDQEAIVGTYHLNGVQA
                     TSYCRIRYTASLDMGRTERQRRVLGMLFDKAKIAGLSSIFKIMDDVFPMVTTSLSKQD
                     ILGLIPTLIGYKFTDSTGFPQKFKFSNIKGSIIVPTDLENNVVELHKFLYDDQDYTPS
                     SEVVARSNKILEIVGGESKLDDAAKTTTQDTDTTNANDTFVWSGDNTSGSTDNSGDYD
                     YDNDYDNGGYDGGYDNGGGDYGGGGDYDNSGDDGSYDNGGDDTGSDTGGGDYDGGDDT
                     GGDETGGDDGFSDDSGASSEEQ"
     gene            complement(7085..8488)
                     /locus_tag="ACZPD3_02230"
     CDS             complement(7085..8488)
                     /locus_tag="ACZPD3_02230"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR1414856.1"
                     /translation="MLKDNEKNFSRLHMIIDAIVLVLSYFLAWLIRFVGPMAATAVRT
                     RSFQQYMLMLVFIVPVYLLLYQAFTLYTPMRMQGRRLVLANIVKANSLGLLILMFTFY
                     MIDESDFSRSTYIMFYVINIVLQWCARMLIFALLRDMRERGLNQKQMICVGYSRAAEE
                     YIDRVLANPQWGYVIRGILDDNVPAGTEYKGIKVLGRIANLNIILPENRLDEIAITLG
                     LSEYYRLEEIVALCEKSGVHTKFIPDYNKIIPTKPYTEDILGLPVINIRYVPLNNTFN
                     ALVKRAMDIAGSIVGIIVTSPLMLLMCAIIKLTSPGPLIYKQERVGLHNQTFRMYKFR
                     SMEVQPESEEKKAWTVKNDPRVTPIGKFMRHTSIDELPQLFNILKGNMSLVGPRPERP
                     FFVEKFREEIPRYMVKHQVRPGLTGWAQVNGYRGDTSIRKRIEYDLYYIENWSIGLDI
                     KIIFLTFFKGFINKNAY"
BASE COUNT         2138 a         2171 c         1500 g         2705 t
ORIGIN      
        1 aaatccagat tcttcagata tttcttggaa ggcattacta cctttgtcag tttattgctt
       61 ccatacttca ctacacgaag atttgtgcat ttgctgagat tcagagttcc tgcaagactg
      121 ttattactca gattcagata tgcaactttt gtattctttg taatatttac agaagtaagt
      181 ttgttatttg ccgcaaacag atctgtcgca taggtaaaac gttcaatacc tttcaggttg
      241 gaaattccaa gtcctgaaac atcaatcgtc ttggctgctt tgatctctgc cgctgaaagt
      301 ttcttatcct tgtctgtatc aatattgtct aaaacatact ggcggaactt tgcatccggg
      361 aagttcgctg aactgactgc tactgctgat gatgcttcag cagcatcgga ctgaaattca
      421 gatccgtcct ctacctccgg agcttcctct tctgctgagc caacagactg ttcttctact
      481 gttgatgtat catcactaaa atcagcagcc tgaacgctga taccagaagt catcatagct
      541 gcactgatca ttcctgcgac taataactga tttaattttc tgtttttcat ctttccctct
      601 cctgttcgta attttcatac cttttgatac gatataaaat tatcatattc catatcaatt
      661 tgcaacatat ttttaattat actgtaatta tctggtaaat ctgtaaaaat aatttaccct
      721 gcagttactt acttctcttc cattgtttct ttttccactg gtgtttcttc caccggctgt
      781 tcctgtgctg gttctgtttc tgctgttttc tctgtctctg gttcttccgg ctgtaattct
      841 tctgccggtg tttcttccgg ctcattactc ccatcttcat cttcatctga agatacagtg
      901 tcattttcca cattcttcgc cggttccgga gtcgggcatg acgttccgca atacggacag
      961 aaacttacat taatatctac ctcccgctcg caggacgggc agattttctc tcctttcagg
     1021 ttcgcagcat tatccttata ctccctgatt ttcagataat gctgatcaat ctcctgacat
     1081 aaagcactta actcactatc aattccatca ccctgagaat gtcgtctgta aacaatatca
     1141 ccgatatcaa ctttgatttt ctcaataatg cgctcttctc ctgtgatttt acttcttatc
     1201 ttctgtgcct cgatcgtctg ctctgcgcgg gcacctaatt ctcttgttgt ctttcccagt
     1261 tccttcgttg tttttgaaaa tgtcttaact aatccatcaa aaaaatctgt tcccataata
     1321 tttactccct tccattattg tcctcataat gcaagaacaa aactatttta attaatgact
     1381 accctccagc aggatcggct cctgtccgcg aagaaggatc ttcggaccac cgtttccttc
     1441 tatgtattct cttgtgatca caacctctcc tatgctgtca tctttcggaa tctcatacat
     1501 gatatccagc atatactctt ccagtatcgc gcgaagtgcc ctggcacctg tatctctctc
     1561 cagtgctttc gcagcaattg catgaagcgc cccgtcttca aattccagtt tcacttcatc
     1621 cagtgcaaga agtttctgat actgtttaag aatcgcattc ttcggctcct gcaggatctt
     1681 cacaagcata tcctcattca atccgtccag agtaaagatg atcggaagtc gtccgataaa
     1741 ttccggaatc attccaaaac tgcggatatc ctccactgtt actttctgaa gcagatgagg
     1801 atcattatca tatttatcct tcaggtctgc ataaaaaccg atagatgcct gcttgttcag
     1861 cctttcttta atgatattct ccagatcagg aaaagcaccg ccacagataa acagaatatt
     1921 acgtgtatta acagttacaa gcggtaccat tgcgtttttg ctgtttgcac ctacaggaac
     1981 ttccacctca cttccctcaa gaagcttcag cattccctgc tgcactgcct caccactgac
     2041 atctctctgg ttggtgttct tcttcttggc aatcttatca atctcatcaa tgaaaataat
     2101 accatgctcg gcacgctcca catcattatc tgccgccgcc agaagtttgg aaacaacgct
     2161 ctctatatca tcgccaatat aaccggcttc tgtaagagaa gtggcatctg caattgcaag
     2221 cggcacatcc agaagtttcg ccagggtttt aaccagatag gttttaccgc atccggttgg
     2281 tccgatcatc agcatattag atttctcaat ttcaatctca tccattgtat ctgttgccac
     2341 acgtttatag tgattgtaaa ccgctacaga cattactttc ttggctttct cctgaccgat
     2401 cacatactgg tcaagggttt ctttgatctt atggggagca ggaatatttt tcagatccag
     2461 aaccggtttt tcctgtttct ttttcttttt ttctttcttt ggctgctgca ccggattctg
     2521 gaagctgccc agatcgatca tgctcacatt cggcatattc aacaggtcag aataattgaa
     2581 cttaccctca ttaaactgct gattcataga attaaagctc ttctgcatac aatccgtgca
     2641 gacatgtatg ttattgggaa gttcgatcat cggaccggcc ttgctctcag gtctgcggca
     2701 gagaaaacag aatttttcgg atttgtttct ctttttctct gtatcttcgc tgctgtctat
     2761 tatttcatca tcattatagt tctcgctcat aatgactcct ttctatatcc tcttattcgt
     2821 aacagtacag tgctcctgca ggatagcagg ctgctggatc attacgctta tttaactgaa
     2881 ttatatcata caattccctt ataacaaaat gaaagtttta taaacacaaa aagaatcctg
     2941 cctgggttac tgttcactga taatattata aaagacaccg ccgcatgggc gatgtctttt
     3001 gttttattca ttgtccggtc tggtgccgag agtcagctct atagtatttt cttcatattc
     3061 cccattagtg gctcttgcaa taactgcctc aatcttctct cccgatttat aatactgcag
     3121 tttatccagc agatcatcac catcactgac tttctgtccg tcgaatttta cgatgatgtc
     3181 gcctttacag atacctgcct cctgtgccgg agaatcatcc tccacacttc ttacaaaagc
     3241 acctgtgggc atattataca tctcaattgc ctctgtggtc agatttgcaa gacttactcc
     3301 cagatatcct tttttgctgc tgtctacttt ctccctggtc tcacgattca tcagttcttc
     3361 cagaataggc tttgccttgg aaataggaat ggcatatccc ataccttcca cagcactgtc
     3421 tgcgtatttt gcagaattga tgccaatcag ctcgcctttc atattcaaaa gtgcaccacc
     3481 actgttaccc ggattgattg ccgcatccgt ctggatcagt cctgtactgt tcgttccgtc
     3541 atctgtagaa atcgtccggt tcaatgcact tatccagcct gatgttacag actgaccata
     3601 tccaagcgca ttaccgatcg caactacctg ctgccctact gccagatcat ccgaacttcc
     3661 tatctgagca atcttgatct gatttaaagt gtcctcaggg atatccgatt tcttcaccgc
     3721 aactactgcc agatcattat cagcatctgt acccttaatc ttggcactga ccgcatcctc
     3781 tacattcaga tctccattat ctcccgcatt cacagtctct gtctctgcat ttgccacatc
     3841 atccccgata aagcagacac tcagagttgt agctccatcc accacgtgat tattcgttgc
     3901 gatcagaagc tcatcatcat tatctccaac gataatccct gaacctgttg acgcactctt
     3961 atattggtgg gaactatatc cgaaaaagct tggtatttcc tgaacactga cagtagtgat
     4021 cgcaactaca gaaggcatgg aagcctgagc cactgctgct actgttccat tctcagagtc
     4081 agacagcacc ttatcggcag tgctgtcatc tgatgccgtt tcctgaagat ccacactgtc
     4141 tgtagtttta atctgcaccg aagacttacc tgtattcagg aatctgtctg ctgcaaaatt
     4201 tgccgcctga aatacaactg ccgccaccag gccaaagatc acagccagtg cgattgtcgc
     4261 ccctgctttt ttctgaaaag aattctgatt tcttctgtgt ccagagggac cggatggttt
     4321 caaaacagtt ctctgttctt catggaactg ataatgttca tatctgggct gctgaccgga
     4381 atcatctgtt ttttcaccca ggtttctttc atcattcatg taatctgctc ccttctgttc
     4441 ctgtattact gtttatcgga acactccccg aaataaacaa tgatctgtaa ctgttttatt
     4501 tctgcacagt tacagcaatt cttctcctga actctggaat cagtatatca attaattgtg
     4561 ttcaaaatgt gacaattatc gtaaattggc gtgtcttaag gtcatcctct tacctttatt
     4621 ttctgttcca gtaatatgga ctcatggtac atgcaagaac aactttccgg ggaagttttc
     4681 tgtatctttt ccccagaaaa tatcctgcat atttcattcc gctctgccag atcagctgag
     4741 gaatcagcca gaagtgtccc gtccttatca gatagcccag gcttcttttt accagacgga
     4801 ttccctcccc ctcagaaggt actccttcaa aaatttccgg atgctctgcc tgggatactc
     4861 ccagatcgaa gtttctgtga aactgctgcc ttccggaata attatgggaa tgatacactc
     4921 tcgcatctgc cgcatagaca actgaatagc ctttctgaat cattgttccg gcacagatca
     4981 tatcttcatt gaaaatcgcc cgctctgtaa aaccgcctgt tttcagataa atccctttat
     5041 cataggccgc acacacatta gaacagaaat aggtcttgat cccgtactta tcaatatcag
     5101 ccagcgactt taccgaggac tgctccggat aattaaaaga acgggtataa cgttccagaa
     5161 agcgacagtc tgccctgggt aattgtcttg cataggctgc tcctgcaccc ggattttcac
     5221 tgacagcaca gacaagattt ccaattactt ttctgtcagc agggagagca tcctgtgtca
     5281 taaagatcat aatatctgca ttcgaaagtt ctgctgccct tcttctggtt ccgccatgat
     5341 caaaatcctt ctgttccagg tgatgaacct caaggatcgg acatttctcc cattctctgt
     5401 tccagtactg ctctcccgta ttcataacca gaatcctgcg aggacggtat tcctgttctt
     5461 ccagcctgtg cagcagttct ccaaattctc tgccgggacg ataggcagga atgattacat
     5521 caactgtcgc agtgtcatta tatttttcct tatgcgtcat cctcaacact ccttatctga
     5581 aaagaggacc atcctttcca acagatggtc ctcttttcta ttatttatat ttttatagca
     5641 gttccctctt actgttcttc agaacttgct ccagaatcat cgctgaatcc atcgtcgccg
     5701 ccggtctcat ctccaccggt gtcatcgccg ccgtcgtagt ctccaccacc agtgtcactg
     5761 ccggtgtcat cgccgccatt atcataactg ccgtcatcac cactgttatc ataatcgcca
     5821 ccgccgccat aatctccgcc gccattatca taacctccat cataaccacc gttatcataa
     5881 tcattgtcat aatcataatc gccgctgtta tccgtactgc cggaggtatt atcgccggac
     5941 catacaaaag tgtcatttgc atttgtggta tcagtgtcct gtgtcgtagt cttggctgca
     6001 tcatccagct tactttctcc gccgacgatc tcaagtatct tattactgcg ggccactacc
     6061 tcagaagacg gagtataatc ctgatcatca tacaggaact tgtgaagttc aactacattg
     6121 ttctcaagat cagtaggaac aataatactt cccttgatat tggagaattt aaacttctgc
     6181 gggaatcccg tactgtctgt aaatttatat ccgatcagag tcggaataag tcccagaata
     6241 tcctgctttg aaagagatgt tgtaaccatc gggaatacat catccataat cttaaagata
     6301 gaagaaagac ctgctatctt cgccttatca aagagcattc caagtactcg tctctgacgc
     6361 tctgtacgtc ccatatccag acttgcagta taacggatac ggcaatagga tgtcgcctgt
     6421 acgccgttca gatgataagt tcctacaatt gcttcctgat cttccggctt tggctccggt
     6481 ttctcgagcg gagtgtagtc ttttcctgtc agcttggatg tttccacgca atagttattc
     6541 atatgttcaa tttccgcata agataacgga atatcaagac cgccaagatc atctatcact
     6601 tctacaagtg cggaaaaatc agccgtcgca taatcggaaa tatccagatc caggtttgta
     6661 tttaacatgg tgatcgcctg ttccggacca cccagcgcat aggctgcatt tgccttcgta
     6721 taggtatcat ctccaacatt gagaagtgta tctctgtata cggaaaccat acgcacttct
     6781 tttgtatcgt tgtttacact cacaatgatc atggtatcac tgttctgtgc agaatactgg
     6841 gagccttccc ctctggtatc aataccaaac agcacatagg tcttgtatcc tgtcatcttt
     6901 ggtgcttcct ggtttacgac tattttttcc tttttcagtt ccggctgctc gatcttatcc
     6961 agcctggaag aaatctgtcc atacacatac agcccgccaa taaacagcag cagtacaatg
     7021 atctcaagca caaacagtac ttttttcttt tttcctggtt tagccatgac tttttcccct
     7081 tatcttaata tgcatttttg tttataaatc ccttgaaaaa tgtaaggaat ataatcttga
     7141 tatccagccc tatgctccag ttttcaatgt aataaaggtc atattcgatt ctttttctta
     7201 tggatgtgtc tccgcggtaa ccattcacct gagcccagcc ggtaagtccc gggcgaacct
     7261 gatgtttcac catatatctc gggatttctt ctctgaattt ctctacaaaa aacggccgct
     7321 ccggtcgtgg tcccacaagg ctcatgttac ccttcagtat gttgaaaagc tgtggcagtt
     7381 catcaatact ggtgtgacgc ataaactttc cgatgggtgt tactcttggg tcattcttta
     7441 ctgtccatgc cttcttctct tccgattccg gctgtacttc catggaacgg aacttataca
     7501 tccgaaaagt ctggttatga agtccaaccc tctcctgttt atagatcagt ggtcccggtg
     7561 atgtcagctt aatgatagca cacattaaca gcatcagcgg tgaggttact atgattccaa
     7621 caatagaccc tgcaatgtcc atcgcccgct tgaccagtgc attgaatgta ttgttcaacg
     7681 gtacataacg gatattgata accggaagtc ccagtatatc ttctgtgtat ggttttgtcg
     7741 gaatgatctt attataatcc gggataaact tggtgtgtac tccagatttt tcacacagtg
     7801 ctacaatctc ttccaggcgg taatattcac ttaagcccag agtgatcgct atttcatcca
     7861 gccggttctc cggaagtatt atattcagat tggcaatcct tccaagaact ttgattccct
     7921 tgtactctgt tcctgccgga acattgtcat ccagtattcc gcggatcaca tatccccact
     7981 gaggattggc aagaactctg tcaatgtatt cctctgctgc gcggctgtat cccacacaga
     8041 tcatctgttt ctgattcagt cccctctctc tcatatctct cagaagagcg aaaatcaaca
     8101 ttcgggcaca ccactgaagc acaatattga ttacataaaa cataatataa gtagaacggg
     8161 aaaaatcgct ctcatctatc atgtaaaatg taaacatcag aataaggagc ccaagcgagt
     8221 tggcctttac aatattagcc aatacaagcc gacgcccctg catccgcatg ggagtataca
     8281 atgtgaaagc ctgatacaac aacagataaa ccggtacaat aaaaaccagc atcagcatat
     8341 actgctgaaa gcttctggtt ctgactgctg ttgctgccat aggaccaaca aaacggatca
     8401 gccatgccag aaaatatgac agtaccagta caattgcatc aatgatcata tggagacgac
     8461 tgaaattctt ttcattgtcc tttagcaatc ttttgtcacc tcattttttt atcg
//
LOCUS       DBMDXG010000039         8453 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2247_length_8453_cov_1.787117, whole genome shotgun sequence.
ACCESSION   DBMDXG010000039 DBMDXG010000000
VERSION     DBMDXG010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2247_length_8453_cov_1.787117"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="ACZPD3_02235"
     CDS             <1..1998
                     /locus_tag="ACZPD3_02235"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1414857.1"
                     /translation="GNILATSNKVYNVILDCKTVNSDEDYVEPTIRALNEVLGIDEET
                     VRSLLADSRTSQSQYQVLTKQLSMDKKKEFEKYKAEDEDSGLTKAQAKERANIKGVWF
                     EEDYLRSYPFNETACDTIGFALDRDVADIGLEGYYNSTLTGVDGRQYGYINDDSDVEQ
                     TIIAAVNGKNIQTSIDIGAQQIVEKYVNGFKEAMGAKNIGVIVENPSTGEIIAMDGGD
                     RYDLNNPRDLSTVYTEDEIKAMNDVETVEALNGMWSNFCVTDAYEPGSVVKPIVMASA
                     LEKGAIQETDTFVCDGSQTFGDTMIKCAVYPSAHGTETLGEVIANSCNDAMMQIGAKM
                     GATQFIKAQSLFNFGTRTGIDLPNEGAGIIHTKDSMGETELACSAFGQGFTCTMIQEI
                     NAMSSVINGGYYYQPHLVTKVLDSNGGTVKTISPILLKQTISSRISSDIRSYMALSVQ
                     QGTSRHSKVQGYSSGGKTGTAEKYPRGNGKYLVSFIGFAPVDDPAVVIYVVVDEPNVE
                     DQANSTYPQYIAQGILSELLPYLNVVPDESEDGTVPETELWEGFKGHLKSTSISDSEL
                     DSDGNLVDADGNLIDWDGNRIDENGYLLDANGDHILDEEGNYKMSTNLVSASGSTDAD
                     SSGDAVSNPDAPAPLEDDEAETTEDNDEETDGITNEEAGLE"
     gene            2068..3903
                     /locus_tag="ACZPD3_02240"
     CDS             2068..3903
                     /locus_tag="ACZPD3_02240"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR1414858.1"
                     /translation="MKKNMTFHKRKIWVVLLCCILMMTGLIGRLVYLMCFRSDYYYKK
                     AKELHEREREIKAARGEILDARGKVLASNRTVCTISVIHSQIKEPEKVIALLSEKLKI
                     DEAEVRKRVEKISSIEIVKTNVEKSTGDEIRECSLAGVKVDEDYKRYYPCGSLASKVI
                     GFTGGDNQGIIGLEVKYEEILRGQPGKILTTTDARGVEIDKLGETREKPIEGKSLIIS
                     LDVNIQEFAQQSALKVMEEKQAERVSILLMNPQNGEIYACVNVPEFDLNDPFTLTDDL
                     NMQLNESGITIPSEDHNEQSELAVQTASGKTNTERKQELLNQMWRNPCLNDTYEPGST
                     FKIITMAAGLEAGVVSPGDRFYCPGYKVVEDRRIHCAKRTGHGSQNFVEGAQNSCNPV
                     FIEVGLRLGVERYYKYFRQFGLLDKTGTDLPGEAGTIMHQKKNMGEVELATVSFGQSF
                     QITPVQLAATVSSLINGGRRVTPHFGVAVLNPDRTEGEKLIFPVKEGIVSEETSKEIR
                     EILETVVSQGSGKNAKIEGYAIGGKTATSQTLPRSANRYISSFLGFAPAEDPQILGLC
                     IIHDPKGIYYGGTVAAPVIRGIFENILPYLGIEKSDVSDFSAEGN"
     gene            4012..4968
                     /gene="mraY"
                     /locus_tag="ACZPD3_02245"
     CDS             4012..4968
                     /gene="mraY"
                     /locus_tag="ACZPD3_02245"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR1414859.1"
                     /translation="MEFQVVIPVLISFAISVVLGPVIIPFLRRLKMGQTERTEGVQSH
                     LKKAGTPTMGGVIFLIATAITALFYVGDYPKIIPVLFLTLGFGIIGFLDDYLKVVLKR
                     SDGLLPWQKFLLQVVLTAIFVFYIVKYTDISLTMRIPFWSGHFLNLGWLAVPVLFFAV
                     IGTVNGVNFTDGLDGLASSVTLIVAVFFTVVSIGMKSGIEPITGAVMGGLMGFLLFNV
                     YPAKVFMGDTGSLALGGFVAGTAYVMQMPLFILIVGLIYLIEVLSVIIQVTYFKATHG
                     KRIFKMAPIHHHFELCGWSETRVVAVFSVITAVMCMVALLAL"
     gene            5023..6378
                     /gene="murD"
                     /locus_tag="ACZPD3_02250"
     CDS             5023..6378
                     /gene="murD"
                     /locus_tag="ACZPD3_02250"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR1414860.1"
                     /translation="MELQGKKVLVFGSGKSGIGASDLLAKVGAFPVIYDGNAETDKDA
                     VVHKTDGTYPVTVYAGELPKEVQDSLDLVVLSPGVPTDLPLVKSFYEQGLPVWGEVEL
                     AYRVGDGEVLAITGTNGKTTTTALLGKIMQDARESVFVVGNIGTPYTSKALEMKQNSV
                     TVAEISSFQLETIDEFAPKVSAILNITEDHLNRHHTMEEYIRVKELITENQGTEDVCV
                     LNYEDEVLREFGKHLTPRVVYFSSGRKLDEGIYLDGNKIILKDGEKEIEVVKTEDLKL
                     LGKHNFENVMAAVAMAYYDGVSLDSIRKSICEFTAVAHRIEYVTEKKGVVYYNDSKGT
                     NPDAAIKGIQAMNRPTLLIGGGYDKQSGYDEWIEAFDGKVRYLVLIGQTKEKIKEAAE
                     KHGFHDIILCEDLKEAVKVCEEKAQPGDAVLLSPACASWGQFDNYEQRGDMFKEYVRN
                     L"
     gene            6465..7562
                     /locus_tag="ACZPD3_02255"
     CDS             6465..7562
                     /locus_tag="ACZPD3_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1414861.1"
                     /translation="MARKRATDLSKTDMTMLVLIVLLVIFGLAVLFSSSEYNGRVRFG
                     DSACYFKKQLFATALGMGVMYMVSSIDYHFFLRLGPVAYLISMFLSGAVLFVGQEING
                     SKRWLNLGPLSFQPSEFAKVAVILFLAWQIERTKKATMGFGFMCRTILTLLPIIGLVG
                     SNNLSTAIIILGIGGILIFVSNPGYLEFIGLGSAGAGFIAVFLAAESYRLERLAIWRN
                     PEKYEKGFQTIQGLYAIGSGGIFGRGFGNSLQKLGFVPEAQNDMIFSIICEEMGAAGA
                     IFLIFLFAMLLWRLGVAAMHAKDLAGALICCGIMGHLALQVILNIAVVTNTIPNTGIT
                     LPFISYGGTSAVFLLGEMGLAMNVGKCDRIN"
     gene            7643..>8453
                     /locus_tag="ACZPD3_02260"
     CDS             7643..>8453
                     /locus_tag="ACZPD3_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR1414862.1"
                     /translation="MDEIHVTGKASLSGEIFIQGSKNAALPMIAASLMHRGLSILRGC
                     PRISDVFCMEEILKSLGAVTWWDDHDLYLDCSCADGTEIPAVYTGRMRSSIILAGAVL
                     ARNRKCRIGYPGGCTIGKRPIDFHLMALRALGADVRENASGLDGECVRFEGQDIVFPK
                     SSVGAAQQAILAAVLAKGVTHLYNCAKEPEVFWLCRYLKTMGALIEGEETGEITIRGV
                     DELKGGDYRIPPDRIVAGTYLCAAAAARSKIILHDVPVEELQSFMEVYRKIG"
BASE COUNT         2552 a         1433 c         2232 g         2236 t
ORIGIN      
        1 ggaaatatac tggctaccag caataaagtt tataatgtga tcctggactg taaaactgta
       61 aattcagatg aggactatgt agagccaaca atacgtgcct taaatgaggt gctggggatt
      121 gatgaagaaa cagtgcgttc tcttttggcc gattcaagaa ccagccagag tcagtatcag
      181 gtactgacaa aacagctgtc tatggacaaa aagaaagaat ttgaaaaata taaggcagag
      241 gatgaagatt ccggtcttac taaagcgcag gcaaaggaac gggcaaatat caaaggcgtc
      301 tggtttgaag aggattatct gagaagttac ccgtttaatg aaacagcatg cgatacaatt
      361 ggttttgcac tggacagaga tgtggcagat atcggactgg aagggtacta taacagcaca
      421 ctgacaggcg tagacggaag acagtatggg tatatcaatg atgattcaga tgtggagcag
      481 accattattg cagcggtgaa tggaaaaaac attcagacca gcattgacat cggtgcacag
      541 cagattgttg agaaatatgt taatggattt aaagaggcaa tgggggccaa gaatataggt
      601 gtcattgttg aaaatccgtc caccggtgag attattgcca tggatggtgg tgaccgttat
      661 gatctgaata atccgagaga tctttccact gtatatacag aagatgaaat caaagcgatg
      721 aatgatgtgg aaacagtgga ggctctgaat ggtatgtgga gtaatttctg cgtcacagat
      781 gcctatgagc cgggatctgt tgtaaagccg atcgtcatgg cttctgcatt ggaaaagggt
      841 gcgatacagg aaacagatac atttgtatgc gatggatcgc agacttttgg agatactatg
      901 atcaagtgtg cggtttatcc gagcgcacat ggcacagaga cactgggaga ggtgattgca
      961 aactcctgta atgatgcaat gatgcagatt ggtgccaaga tgggagctac tcagtttatc
     1021 aaagcacaga gtctgtttaa tttcggaaca aggactggga ttgacctgcc gaatgaaggc
     1081 gcaggtatta tccatactaa ggacagtatg ggggaaacag aacttgcatg ttctgctttt
     1141 ggacagggtt ttacctgtac catgatccag gaaatcaatg cgatgagttc cgttatcaat
     1201 ggcggatatt attatcagcc gcatcttgtg acaaaggttc ttgacagtaa tggcggaact
     1261 gtaaaaacaa tctcgccgat acttctgaag cagacgattt cctccaggat ttccagtgat
     1321 atcagaagct atatggcatt gagtgtacag cagggtacca gccgtcattc caaagtgcag
     1381 ggttacagtt caggcggtaa aacaggtaca gcggagaaat acccgcgagg aaacggaaaa
     1441 tatctggttt cttttattgg atttgctcct gtagatgacc cggctgtggt aatttatgta
     1501 gttgtagatg aaccgaatgt agaggatcag gcaaacagta cttatccgca gtatattgca
     1561 cagggaatcc tttcggagct tcttccgtat ctgaatgtag tgccggatga atctgaagat
     1621 ggaacagtgc cggagacaga attgtgggaa gggtttaagg gacatttaaa gagtacttcc
     1681 atatcggaca gcgaacttga cagtgacggt aatctggtgg atgcagatgg caatctgata
     1741 gactgggatg gaaaccggat tgatgaaaac ggatatctgc tggatgccaa tggagatcac
     1801 atattggatg aggaaggcaa ttataagatg tctacaaatc ttgtcagtgc ttcgggaagt
     1861 acggatgcag attcttcagg agatgcagta tccaatcctg atgcacctgc acctctggag
     1921 gatgatgaag ctgagacgac tgaggataat gacgaagaga cagacggtat taccaatgaa
     1981 gaagcaggtc tggaataaaa acgtatagtt atagctccgc agagaatggt catatattgt
     2041 tatgacaatc tctgcggagc tttttttatg aaaaaaaata tgacgtttca caagagaaaa
     2101 atatgggtgg tattactctg ctgtatttta atgatgacag gccttatcgg aagactggta
     2161 tatcttatgt gtttcagatc agactattat tataagaaag caaaagaact tcacgaaaga
     2221 gagagagaga tcaaagcggc cagaggggag attctggatg caaggggaaa agttctggca
     2281 tcaaacagga cggtatgtac aatctctgtg atccacagtc agataaaaga acctgaaaaa
     2341 gtaatcgccc tgctgtctga aaaactgaaa atagatgagg cagaagtgag aaaaagagtg
     2401 gagaaaatct cttccataga aatagtaaag acaaatgtgg aaaaaagtac aggtgatgag
     2461 ataagagagt gcagtctggc aggggtgaaa gttgatgagg attataagcg ttactatcca
     2521 tgtggaagtc tggcttcaaa agtcattggc tttacgggag gtgataatca gggaattatc
     2581 ggtctggaag tgaaatatga agaaatcctg aggggacagc cgggtaaaat cctgacaaca
     2641 acagatgcca ggggtgtgga aattgataaa ttgggtgaga caagggaaaa accgatagag
     2701 gggaaaagcc tgataatcag ccttgatgta aatattcagg aatttgcgca gcagtcagct
     2761 ttgaaagtaa tggaggaaaa gcaggcagaa cgtgtatcta tcctgctgat gaatccacag
     2821 aacggcgaaa tatatgcatg tgtcaatgtg ccggaatttg acctgaatga tccttttacc
     2881 ctgacagatg atctgaatat gcagttaaat gaatccggga taacaatacc gtcggaagat
     2941 cacaatgaac aaagtgaact ggcagtgcag acagcatcag gaaaaacaaa tacagaaaga
     3001 aagcaggaat tactgaatca gatgtggaga aatccctgcc tgaatgatac ttatgaaccg
     3061 ggctccacat ttaaaatcat caccatggca gccggactgg aggctggtgt tgtttcacct
     3121 ggggaccgtt tttattgtcc cggatataaa gtggtggagg atcgcagaat ccactgtgca
     3181 aaaagaacag gacatggatc ccagaatttt gtggaaggtg cacagaattc ctgtaaccct
     3241 gtgtttatag aggtggggct tcgccttggg gtagaacgtt attataagta tttcagacag
     3301 tttggacttc tggataaaac cgggacagat cttccgggag aggcaggaac tattatgcat
     3361 cagaagaaaa atatgggaga agtggaactg gcaacggttt cttttggaca gtcttttcag
     3421 attacgcctg ttcagctggc tgcaacagta agttcactta taaacggagg cagacgggtg
     3481 acgccgcatt ttggagtcgc agtgttaaat cctgacagaa cagagggtga gaaactgatt
     3541 tttccggtaa aggaaggaat tgtctctgaa gagacttcga aggaaataag agaaatcctg
     3601 gaaacagtag tatctcaggg ctccggaaaa aatgcaaaaa ttgaaggata tgccatcggt
     3661 gggaaaactg ccacttctca gacactgcca agaagtgcaa atcgttatat ttcctctttt
     3721 ttgggatttg cacctgcaga agatccgcag atactgggac tgtgtattat tcatgatccg
     3781 aaaggaatct attatggagg gacagtagct gcacctgtga ttcgtggtat ttttgagaat
     3841 atcctgccat atcttggaat tgaaaaaagt gatgtttctg atttttctgc agaaggcaat
     3901 tagggaaatt ccgcaggcag ggttgagaaa taagcaaaaa agccttatac taaaagaaat
     3961 ctgaaagtaa aaaactttca ggaaaaaaca aaagaatgaa cgaggtgtaa gatggaattt
     4021 caagttgtaa tccctgtgct gatttctttt gcaatcagcg ttgtattagg accggtcatc
     4081 attccttttc ttcggagact gaagatgggc cagacagaaa gaacagaggg cgtacagtcc
     4141 catttaaaga aagcaggaac acctacaatg ggtggagtga tctttctgat tgccacagcg
     4201 ataactgcat tattttatgt aggggattat ccgaagatca ttccggtact gttcctgact
     4261 cttggatttg gaattatcgg atttctggat gattatctga aggttgtgct gaaacgctca
     4321 gacggactgc tgccatggca gaaattttta cttcaggtag ttctgactgc aatttttgta
     4381 ttttatattg ttaagtatac agatatctca ctgactatga ggatcccatt ctggagcgga
     4441 catttcctga atcttggatg gctggcagtt ccggtccttt tctttgctgt gatcggtact
     4501 gtgaatggag ttaattttac ggatggtctg gatggtcttg cttccagtgt gactctgatc
     4561 gttgcagtat tttttacagt agtttctatt ggaatgaaat cggggatcga gccgatcaca
     4621 ggtgcagtta tgggaggact gatgggattt ctgctgttta atgtatatcc ggcaaaagtg
     4681 tttatgggag acacaggttc tctggcattg ggcggttttg ttgcaggtac ggcttatgtg
     4741 atgcagatgc cgttatttat cctgatcgtg ggtctgatct atctgatcga agtactttcc
     4801 gttatcattc aggtaactta tttcaaggca acacatggaa agcgtatttt taagatggca
     4861 ccgatccatc atcattttga gttatgcgga tggtctgaaa cacgtgtagt tgcggtattc
     4921 tcagttatta cagcggttat gtgtatggtc gcactgctgg cactgtaaga ttttggctaa
     4981 gcagatttca gcgttatgct gataaaaggg aggaaaatta ttatggaatt acagggaaaa
     5041 aaggtacttg tattcggttc cggaaaaagc ggaatcggag catctgacct tctggcaaag
     5101 gtaggagcat ttccggttat ctatgacgga aatgccgaaa cagataaaga cgcagttgta
     5161 cataaaacag atgggacata tccggtcact gtatatgcag gagaacttcc gaaagaagta
     5221 caggacagtc ttgatctggt agttttaagc cccggagtgc cgacagatct tccacttgta
     5281 aagagttttt atgagcaggg gcttccggta tggggagagg tagaactggc atacagagta
     5341 ggtgacggcg aagtacttgc catcacagga acaaacggaa aaactactac aacagcactt
     5401 cttggaaaaa ttatgcagga tgcacgtgag tctgtttttg tagtcggcaa tatcggaacc
     5461 ccatatactt ccaaagcact ggagatgaag caaaactctg taactgttgc agaaatcagc
     5521 agttttcagc tggaaacgat tgatgagttt gcgccaaagg tatctgcaat cctgaatatt
     5581 acagaggacc atctgaaccg tcatcataca atggaagagt atatacgtgt gaaagaactg
     5641 atcactgaga atcagggcac agaagatgta tgcgtgctga attatgaaga tgaggttctg
     5701 cgtgagtttg gaaaacatct gactccacga gtggtatatt tctccagcgg acgtaaactg
     5761 gatgagggaa tttatctgga tggaaacaaa atcatcctga aggacggaga aaaagaaatc
     5821 gaagtagtta agacagaaga tctgaagctt cttggaaaac acaattttga gaatgttatg
     5881 gccgcagttg caatggctta ctacgatgga gtatccttgg acagcatccg taagagcatc
     5941 tgtgaattta cagctgttgc acacagaatc gaatatgtga cagagaaaaa aggcgttgta
     6001 tattataatg attctaaagg aactaacccg gatgcggcaa ttaaaggaat ccaggcaatg
     6061 aaccgtccga cacttctgat cggcggcggt tatgataaac agtccggtta tgatgaatgg
     6121 attgaggcat ttgacggaaa ggtacgttat ctggtgctga tcggtcagac aaaggagaaa
     6181 atcaaagaag ctgctgagaa acatggtttc catgacatca tcctctgcga agatctgaaa
     6241 gaggcagtaa aggtatgtga ggagaaagca cagcctggag atgctgtttt gctttctccc
     6301 gcatgtgcaa gctggggaca gtttgataac tatgaacagc gtggagatat gttcaaggaa
     6361 tatgtaagaa atctgtagaa tatctataag taagaattat agctgtccgg cagagaaaga
     6421 cgaagctgca gtgaccggct atagataatt ttgcggagaa acagatggcg cgaaaaagag
     6481 cgacagattt atcaaaaaca gatatgacga tgctggtact gatagtactg cttgtgattt
     6541 ttggcctggc agtattattc agctccagtg aatataatgg cagagtacgt ttcggcgact
     6601 ctgcctgtta ttttaagaaa cagttatttg ccacagcatt gggtatggga gtaatgtata
     6661 tggtttcttc cattgattat catttttttc tgaggctggg accagtggca tatctgattt
     6721 caatgttttt gtccggcgca gtactttttg taggtcagga gatcaacggc tcaaaaagat
     6781 ggctgaatct tggaccttta tcttttcagc cttcagagtt tgcgaaagtt gctgtaatcc
     6841 tttttcttgc atggcagatc gagcgtacaa aaaaagcaac catgggcttt ggatttatgt
     6901 gcaggacaat attgaccctg ctgccgatca ttggtcttgt aggttcaaat aatctgagta
     6961 cagcgatcat tattctgggt atcggtggaa tcctgatttt tgtatccaat ccgggatatc
     7021 tggaatttat cgggcttggc agcgcaggtg caggtttcat agctgttttt cttgctgcag
     7081 agagctatcg tctggaacgt cttgcaatct ggagaaatcc ggagaaatat gaaaaaggat
     7141 ttcagacgat ccagggactg tatgccatcg gcagcggcgg aatattcggc agaggatttg
     7201 gaaacagtct gcagaaactt ggatttgttc cggaagcgca gaatgatatg attttttcga
     7261 ttatatgtga ggaaatggga gcagccggag ctatctttct gatttttctg tttgcaatgc
     7321 ttctgtggcg gctgggtgtg gctgccatgc atgcaaaaga tctggccgga gctcttatct
     7381 gttgcgggat tatggggcat ctggctcttc aggttatttt gaatattgca gtggtgacca
     7441 atacaatccc caatacaggc attacgctgc cttttatcag ctatggagga acttcggcag
     7501 tgtttcttct tggggaaatg ggacttgcga tgaatgttgg gaaatgtgac aggataaatt
     7561 gaattacagg tttcagaatg gcgtaacata taatagaaaa agccataaac tgtaaagtaa
     7621 gagactcttt gggagcaaaa ggttggatga aatccatgtg acaggtaaag catctctcag
     7681 cggggagatt tttatacagg gatcaaaaaa tgcagcactt ccaatgatag ccgcatctct
     7741 gatgcacaga ggactaagca ttctgagggg atgtcccaga atttcagatg ttttctgtat
     7801 ggaagagatt ttgaaaagtc ttggtgcagt gacctggtgg gacgatcatg atctttatct
     7861 ggattgttcc tgcgcagacg gaacagaaat tcctgcagtc tatacaggca gaatgcgttc
     7921 ttcgatcatt ctggcaggtg cggtgcttgc aagaaacaga aaatgcagga tagggtatcc
     7981 gggcggatgt acgatcggga aaagaccaat agatttccat cttatggcac tgagagcact
     8041 tggcgcggat gtccgggaaa atgcatcagg cctggatgga gaatgcgtca gatttgaagg
     8101 gcaggatatt gtgtttccga aatcgagtgt gggagctgct caacaggcaa tactggcagc
     8161 agttcttgca aaaggtgtaa cacatcttta taattgtgcg aaagaaccgg aggttttctg
     8221 gctctgcaga tatctgaaaa caatgggagc actgatagaa ggagaagaaa ccggtgaaat
     8281 tacgatcaga ggagtggatg aactgaaagg cggagactac cgtattcctc cggatcgtat
     8341 tgttgcaggt acatatctgt gtgcggctgc ggcagcaaga agtaagatta ttcttcatga
     8401 tgtacctgtg gaggagttgc agtcgtttat ggaagtatat cggaaaatag gtg
//
LOCUS       DBMDXG010000040         8453 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2248_length_8453_cov_1.553879, whole genome shotgun sequence.
ACCESSION   DBMDXG010000040 DBMDXG010000000
VERSION     DBMDXG010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2248_length_8453_cov_1.553879"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(34..1083)
                     /locus_tag="ACZPD3_02265"
     CDS             complement(34..1083)
                     /locus_tag="ACZPD3_02265"
                     /inference="COORDINATES: protein motif:HMM:NF016218.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HIR1414863.1"
                     /translation="MMLTGAICCSSCFCETAFAKTSKVRVYDVEKGTTSFTSFTYSDN
                     KKDESLAPSEGTGAISSASQKVVIFHQADGSTIIRKADSNGKVTLPAIRNQTGYTFLG
                     WSTKPDQTQNPQYQAGQVIQVRKKTHLYAVMYNWQQEPDIQVNNLAAQLSEYSGIIFV
                     GDSRTYFMQKTLLREYGKDAVAKVSFVCKTGEGLSWFETAGERVMRSEIARLQSDSDK
                     PVAVIFNLGVNDLSSHNSGNGVDYKGEANAYLARMNTLAEELESDCRLFYMSVNPVNT
                     AMKPTRKEAQLRYFNDRLQSRLNKRFQWIDTYKYLMKNGYSTYNEFKGNIDDGVHYST
                     RTYKRIYKYCMNAIR"
     gene            complement(1239..1427)
                     /locus_tag="ACZPD3_02270"
     CDS             complement(1239..1427)
                     /locus_tag="ACZPD3_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414864.1"
                     /translation="MAAVCVAIFCLGQKGTRKKIFFYAGYWLEWFWGNNYQVEKREKV
                     KFTDMDYDCIMIMYVQYI"
     gene            complement(1439..3676)
                     /locus_tag="ACZPD3_02275"
     CDS             complement(1439..3676)
                     /locus_tag="ACZPD3_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HIR1414865.1"
                     /translation="MKKWAPRVLLAAALAGLSAFLLKGDVWTFWTWWLLAFLMGMVAM
                     PVTGRLFSGFEDKGWMFSKVLAITVTGFLTWFLVTAKILPFTAATCIGVSVVCAVGCG
                     VLYHFQGKNGIDCFPAGKVDLIYGEEILFFIFFLIWTYFAGFRPQAYGTEKFMDYGFM
                     EAMMRSTTLPARDLWYSEGTINYYYGGQYFAVFLTKLTGSKVELTYNLMRTFVAAFAF
                     VLPFSLVRQMSVDRLKGSLTGKKRCVPAVAGIIAGLSVSIAGNMHYVVYSKVIPWLQK
                     LQGKEADSYWFPDATRYIGYNPDVPDKTIHEFPCYSFVLGDLHAHVVNVMFVLFLVGF
                     LYAWMRSVRMREAVIMKPGRKQFWKKQLLIPHILLAAVMIGMFRFTNFWDFIIYFVVT
                     GGVVLFTNIVQFDGKVKRILAVTAVQAVEIIVISYVVSLPFTLQFDSMFKGVGIAQNH
                     SMIHQLLILWGLPVVLTVLLIICIIWEKLRGGANRSLYKLMKAISVPDLFAIVMGLCA
                     IGLIVIPELVYVRDIYENGNARANTMFKLTYQAYMLFGMTMGYGIFRMLVSARKKVFK
                     VVSVIGLVLLCWTFGYFGNSVYAWFGKVWEPSEYKGLNATSFLSNDFTEDVAGIKWLK
                     KNVKGSPVVLEANGDSYSGYERVSAMTGLPTVLGWYVHEWLWRDDTADLNEKSADIES
                     IYTSLDEEYVKELLEEYDVSYIFVGSKEREKYGEALNEDVLKSLGEVVFQDEVYSTYI
                     LKVNK"
     gene            complement(3816..5828)
                     /locus_tag="ACZPD3_02280"
     CDS             complement(3816..5828)
                     /locus_tag="ACZPD3_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414866.1"
                     /translation="MLGIIYLLLAGMLGCEASKMLTGEGKSVSGINRIWLMLPASFGV
                     GTLLLTWMVYIISWFFSVVGKAENPLLYGNIIGMSGAAVIMILLSVQKYRKQGSYRIW
                     NIDKTQDKRRLKKEILFFGLLTVFITYMMFYVFYIKDGILYSGLTVYGDYAPHTAMMR
                     SFSAGNNFPTQYPHYGGADVKYHFMFQFLTGNLEYLGMRMDFAYNIVSTLSLVGFLML
                     LYQLALRITGKMCCGVLALFLFFFRSGMAFFRFVWEHIQAGNLVETLEENTSFIGYTV
                     NENWGLWNFNVYLNQRHLAFGLLMVTLALYLFMDWLEAGTAHEEKGILWIKNRIFSKE
                     GWKSRNLDQALLMGMFLGLCAFWNGAAVIGGLLILCGFAIFSDGKVDYAVMAGVSIFF
                     SWLQSKIFIVGSAMSPQIYLGFLAEDKTVPGVVKYLFWMSGVFFLGLVLMVWFMRRRE
                     RAILVSFLFPVIFAFVLLMTPDINVNHKYIMISYAFLTIFWAWAVCSLWKGRNGQSGI
                     QKTMGKILAIVLVISLSVTGIYDFVVIIKGNGPGRRVTVNMNSELTQWLEENLEKNDL
                     LLTPEYSMNEVTMSGAMLYCGWPYYAWSAGYDTNYRAAQAVTIYTTSDSETLKKTVQQ
                     EKITYILFEEGSEFEQQECREETIAAAYEKVYETQDGRIRIYKTTE"
     gene            complement(5952..6647)
                     /locus_tag="ACZPD3_02285"
     CDS             complement(5952..6647)
                     /locus_tag="ACZPD3_02285"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR1414867.1"
                     /translation="MKKYGLELMLGCLLLVSFLILSKQAAEVSETMSSTENSKTILVD
                     AGHGGADPGMIGVNGLEEKGINLQIAVKLKDSLEKQGFSVIMTREEDKGLYEEDSRNQ
                     KAQDMQCRIAMIKKYRPVLCISVHQNSYQDSSVCGPQVFYYEDSVRGKNLAEFIQEEL
                     NLGLKVKRPRVAKGNKTYYLLKRSESVLNIVECGFLTNPEEAGLLCKEEYQNKIVEAI
                     VKGIEQYLKQQKI"
     gene            complement(6812..>8453)
                     /locus_tag="ACZPD3_02290"
     CDS             complement(6812..>8453)
                     /locus_tag="ACZPD3_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR1414868.1"
                     /translation="KYKPVIAVAVLVILVAILGIVTHVVMKYIPSSEKMDLNEYYGEM
                     ADGEIALVIGTEKLEERGLVDGDRVYLPLDVVNTYLNQRYYWDSANQQILYATPSELT
                     SVSASSEAGDKVWVKDDKVYLNLTYVQEFTDLDAYITKDPYRIAIQYKFKNVKTVTVK
                     KNTSIRYRGGIKSAILTSVKKGTKLRLIEEMENWDQVATDDGYIGYIDKKKVGEAEKT
                     KFERSFKKEEYSYLTMDSKINMVWHQVTSTDANAYFADATANMTGVNVISPTWFYLTD
                     TSGNIASIASADYVSQAHEKGLQVWGLIDNFTQEVSTIETLSSTAARQNIISQLIQAA
                     KDVGMDGINVDFESLSEDVGIHFLEFLRELSIECHKNNLVLSVDNPVPEDFTSHYDRA
                     EQGRVVDYVIIMGYDEHYVGSEAGSVASLPWVEQGIQDTLNEVPAERVINAIPFYTRL
                     WRTTGGNVTSEAIGMDQAQQTIADNNVETYWDKTTSQNYGKYDIDNSTYQIWLEDAQS
                     VAEKVKLVSKYDLAGVSAWKLGFENNGIWQVISDNLNN"
BASE COUNT         2460 a         2210 c         1295 g         2488 t
ORIGIN      
        1 caagattata atttgtgtat aaatcaaaaa gcattatctg atcgcattca tacaatattt
       61 atagattctt ttataggtac gtgtagaata gtgaacgccg tcatctatat ttcccttaaa
      121 ctcattgtat gtactgtaac cattcttcat cagatattta taggtatcta tccattgaaa
      181 tctcttattt aacctgctct gcaatctgtc attaaagtaa cgtaactgcg cttcttttcg
      241 agttggtttc attgctgtat ttaccggatt cacagacata taaaacaatc tgcagtcact
      301 ttcaagttcc tctgcaagag tgttcatacg cgcaagatat gcatttgcct cgcctttata
      361 atcaacccca ttcccggaat tatgactcga aagatcattc acaccaagat taaaaataac
      421 tgctacgggc ttatctgaat ctgactgcag acgggcaatt tcacttctca ttactctctc
      481 tcctgctgtt tcaaaccagc tcagtccctc ccctgtctta cacacaaatg atacttttgc
      541 aactgcatcc tttccatatt cccgaagaag agttttctgc ataaaatacg ttctggaatc
      601 tcccacaaaa ataattccgg aatactccga cagctgagct gccagattat tgacctgaat
      661 atccggctcc tgttgccagt tatacataac tgcatataaa tgggttttct tccttacctg
      721 tatcacctgt cccgcctgat actgcggatt ctgagtctga tccggcttcg tgctccatcc
      781 aagaaaagta tatcctgtct gatttcttat tgctggaagt gtcacttttc cattactgtc
      841 tgcttttctt attatcgtag acccatctgc ctgatggaaa ataaccacct tctgagaagc
      901 agatgaaatc gctcctgttc cctccgacgg tgccagtgat tcatcctttt tattatccga
      961 atatgtaaaa gatgtgaagc ttgtggttcc tttttccaca tcgtaaaccc ttactttgct
     1021 ggttttcgca aaagcagttt cacaaaaaca tgaactacag caaattgctc ctgtcaacat
     1081 cataatcatc attttcctgc tataatgtct aacctgaatt attcgattcc tcattttaaa
     1141 actcctttcc tgtcatgtaa atattcacaa tcctcatttt ctcctactac ttatacagtg
     1201 tcatcttttc atttttccac tgtagcaaca tatttttctt atatatattg cacatacatt
     1261 atcataatac aatcgtaatc catatctgta aattttactt tttccctctt ttccacctga
     1321 taattatttc cccaaaacca ttccagccaa taaccagcgt aaaaaaatat ttttttacgc
     1381 gtcccttttt gacccaaaca aaaaatggcg acacaaactg ccgccatttt tcgccctctt
     1441 acttattaac tttaagaata taagtactat acacctcatc ctggaacacc acttctccaa
     1501 gtgacttcaa cacatcctca ttcaatgcct ccccatattt ctccctctcc ttcgatccga
     1561 caaaaatata agaaacatca tactcttcca gcagctcctt cacatactcc tcatccaaag
     1621 aagtataaat actctcaata tccgcgctct tctcattcaa gtccgcagta tcatctctcc
     1681 acagccactc atgcacatac catcccagca cagtcggaag ccctgtcata gcagaaacac
     1741 gctcataacc actatagctg tccccgttcg cctcaagcac aaccggtgac cctttcacat
     1801 tcttcttcag ccatttaata ccagccacat cctctgtgaa atcatttgac aggaaagatg
     1861 tcgcattcaa tcctttatat tcagaaggct cccatacctt tccaaaccac gcatacacac
     1921 tgtttccaaa ataaccaaag gtccagcaaa gcaataccag gccgatcact gacacaacct
     1981 taaacacttt cttacgtgca gacaccagca tacggaaaat accatatccc atggtcatcc
     2041 caaacagcat atacgcctga taagtcagct taaacatagt attcgccctg gcgtttccat
     2101 tttcgtaaat atctcttaca taaaccaact ccggaatcac gatcagcccg atcgcacaga
     2161 gtcccatgac aatcgcaaac agatccggca cagagattgc tttcatcagt ttatatagac
     2221 ttctgttcgc accaccacga agcttctccc agatgataca gatgatcagc agaactgtca
     2281 gaaccacagg cagtccccac aaaatcagta gctgatggat catagaatga ttctgcgcaa
     2341 ttcccacacc tttaaacatg ctgtcaaact gcaaagtaaa cggcagagaa acaacgtatg
     2401 agatcacaat gatctccact gcctgaaccg cagtcactgc cagaatcctc ttcactttac
     2461 catcaaactg aacgatattg gtaaacagca ccactccacc ggtcaccacg aaataaataa
     2521 taaaatccca gaaattcgta aaccggaaca taccgatcat cactgcagcc agcaggatat
     2581 gcggaatcaa cagctgtttt ttccagaact gtttccggcc cggtttcata attactgctt
     2641 ctctcatccg cacacttcgc atccacgcat aaagaaatcc caccagaaac agcacaaaca
     2701 ttacatttac cacatgggca tggagatctc ccagcacaaa ggaataacat ggaaattcat
     2761 ggatcgtctt gtccggcaca tccggattgt atccaatata acgggtcgca tccgggaacc
     2821 agtagctgtc cgcttctttt ccctgcagct tctggagcca tggaatgacc ttactgtaaa
     2881 ccacataatg catatttccc gcaattgata cagaaagtcc tgctatgatc ccggccactg
     2941 ccggcacaca acgtttcttt cctgtcaggc ttcctttaag cctgtccaca gacatctgac
     3001 gaaccagtga aaacggaagc acaaacgcaa atgcagccac aaaagtacgc atcagattgt
     3061 aagtgagttc tactttactg ccagtcagct tcgtcagaaa tactgcaaaa tactgtccgc
     3121 cataataata attgatcgta ccttctgaat accacagatc tcttgcagga agtgtcgtac
     3181 ttctcatcat ggcttccatg aaaccataat ccataaattt ctcagttccg tatgcctgcg
     3241 gacggaatcc tgcaaaataa gtccatatca ggaaaaatat aaagaacaga atttcctctc
     3301 cgtaaatcag atctaccttt ccggccggaa agcagtcaat tccatttttt ccctgaaaat
     3361 gatataaaac cccacagcca acagcacata caacagacac accgatacat gtcgctgcag
     3421 taaatggcag tatcttcgca gttaccagaa accacgtaag aaaacctgtc acagtaatcg
     3481 ccagcacttt tgaaaacatc cagcctttgt cttcaaatcc ggaaaacagt ctccctgtca
     3541 caggcatggc aaccattccc atcagaaatg ccaacagcca ccaagtccag aatgtccata
     3601 catctccctt taacagaaat gcagacagcc ctgcaagagc tgctgcaaga agcactctcg
     3661 gtgcccattt cttcatccgt acgtcctcct taatcattta taaaattatt gtggttacta
     3721 taatcttaca cattctctct tatctctgta aatctgtgat cacccggctc ataatcataa
     3781 atccggagta atccccgtaa taatgcattt gaggattatt ctgttgtttt atagatccgt
     3841 atccgtccat cctgagtctc ataaaccttt tcatatgctg cagcaattgt ctcctcacga
     3901 cattcctgct gttcaaattc cgaaccttct tcaaacagga tatatgttat tttttcctgt
     3961 tgtactgttt ttttcaatgt ttcactgtca gatgtggtgt atatcgttac agcctgtgca
     4021 gccctgtaat tcgtatcata gcctgcagac catgcgtaat atggccatcc acagtaaagc
     4081 atagctccgg acatcgtcac ttcattcatg ctgtactcag gagtaagtaa aagatcgttt
     4141 ttctccagat tctcctccag ccattgggtc agctcgctgt tcatatttac agtcacacgt
     4201 ctgcccggac catttccctt tatgatcacc acaaagtcat aaatcccggt cacactcaga
     4261 gatatcacca atactattgc cagtatcttt cccatagttt tctgtatccc ggactgccca
     4321 tttctccctt tccaaagact gcatactgcc catgcccaga aaatcgtcag aaacgcatat
     4381 gatatcatga tatatttatg gtttacatta atatccggtg tcatcagaag cacaaaagca
     4441 aaaatcaccg gaaacaggaa actcacaagg attgctctct cccgtcttct cataaaccat
     4501 accattagta ccagcccaag gaaaaaaact ccgctcatcc agaacagata tttcacaact
     4561 cccgggacag tcttatcctc tgccagaaat cccagataaa tctgaggact catagcactt
     4621 ccaacaataa aaatctttga ctgaagccag gaaaagaaga tggatacccc cgccatgaca
     4681 gcgtaatcca cttttccatc cgagaaaatc gcaaagccgc acaaaatcag cagaccgccg
     4741 atcactgctg ccccattcca gaacgcacac agacccagaa acattcccat aagcagtgcc
     4801 tgatccagat ttctgctttt ccatccttcc ttcgaaaaga ttctgttttt tatccagagt
     4861 attcccttct cttcatgtgc cgtcccggct tccagccagt ccataaacag atatagtgcc
     4921 aaagtcacca tcagaagtcc aaatgccaga tgcctctgat taagataaac attaaagttc
     4981 cacaatcccc agttctcatt gacagtatat ccgataaagg aagtattttc ttccagggtt
     5041 tccacaagat tccctgcctg gatatgctcc catacaaatc tgaaaaaagc cattccgctt
     5101 ctgaaaaaga acagaaacaa tgccagtaca ccgcagcaca ttttgcctgt gatcctaagt
     5161 gcaagctgat ataacagcat aagaaatccc acaagcgaca aggtacttac gatattatag
     5221 gcaaagtcca tacgcattcc cagatattcc agatttcctg tgagaaactg aaacataaaa
     5281 tgatacttta catctgcacc tccataatga ggatactgtg tgggaaaatt atttcctgca
     5341 gaaaaagaac gcatcatcgc cgtatgtggt gcataatctc catacacagt caggccggaa
     5401 tacaggattc cgtcttttat atagaacaca taaaacatca tatatgtaat aaatacagtc
     5461 agcagaccaa agaataatat ttcctttttt aatctgcgct tatcctgtgt tttatcaata
     5521 ttccatatcc tgtaacttcc ctgttttctg tatttctgta cgctgagcag tatcataata
     5581 actgctgcac ctgacatccc gataatattt ccatacaaaa gtggattttc agcttttccc
     5641 acaacactga aaaaccagga aataatatat accatccagg tcagtaaaag tgttccgact
     5701 ccaaaagaag ccggaagcat gagccagata cgattgatac ctgacacact ctttccctct
     5761 cctgtcagca ttttcgatgc ttcacagccc agcattcctg ccagaagcag ataaataatt
     5821 ccaagcataa tttctccctg tacatttccc cgccatgcag atactcctat ctccggggga
     5881 ttttatcaaa caagtatcag tatagcaaaa atccctactt attaaaagca agttcacata
     5941 aattaaacat tttaaatttt ttgttgcttc agatattgtt caatcccttt cactatagct
     6001 tccactattt tattctgata ttcttctttg cagagaagtc cggcctcttc cggatttgta
     6061 agaaaaccac actctacaat attcaaaaca ctttcacttc tttttaacag ataataggtt
     6121 ttattacctt ttgccacacg agggcgtttt actttcagtc ccagattcag ttcttcctgg
     6181 ataaactctg ccagattctt tccccggaca gaatcttcat aataaaacac ctggggaccg
     6241 catacagacg aatcctgata gctgttctga tgaacactta tgcacagcac aggtctgtat
     6301 ttctttatca tagcaatcct gcactgcata tcctgtgctt tctgatttct gctgtcctcc
     6361 tcataaaggc ccttatcttc ttcccttgtc atgattacag aaaatccttg tttttccaga
     6421 gaatccttta attttacggc aatctgaaga tttatcccct tttcttccag tccgttcact
     6481 ccgatcattc ccggatccgc tccaccatgt cctgcatcta ccagaatggt ttttgaattt
     6541 tctgttgaac tcatagtctc tgaaacttca gctgcctgtt ttgacagaat gagaaaactg
     6601 acaagaagca gacaccctaa cattaattcc agtccatatt ttttcaaact aaaaacacct
     6661 cctgagacat cttttacagt ctacgggaaa attcactgtt cctgtagact ggctatagca
     6721 attccataaa ataatgcaat cagtatttca taaaattcta tattaatata tgtttcagaa
     6781 gatgttttta tagcctgctg atgcagaact tttaattatt cagattgtcc gagattacct
     6841 gccagattcc gttgttttca aatccaagct tccatgcaga aactcctgca aggtcatatt
     6901 tggataccag cttcactttc tctgcaactg actgagcatc ctcaagccag atctgatagg
     6961 tagaattatc tatatcatat tttccataat tctgggaagt ggtcttatcc cagtaggttt
     7021 ccacattatt atctgcaatt gtctgctgcg cctgatccat accgattgct tcactggtta
     7081 catttccccc tgtggtcctc cacagtctgg tataaaacgg aatggcattg atcacacgtt
     7141 cagccggaac ctcattcagt gtatcctgga ttccctgttc cacccatgga agagatgcca
     7201 cagaaccggc ttcagatcct acataatgct cgtcatatcc catgatgatc acataatcta
     7261 caacccttcc ctgctcagcc cggtcataat ggcttgtaaa atcctcagga accgggttat
     7321 ccactgaaag aaccagatta ttcttatggc actctatgga aagttctctc agaaattcca
     7381 gaaaatggat tcccacatcc tcgctgagag attcaaaatc cacattaatg ccatccattc
     7441 ccacatcttt ggctgcctgg atcagctgac tgataatatt ctgtctcgcc gcagtgctgg
     7501 aaagtgtttc aattgtactt acttcctgtg taaagttatc gatcagaccc catacctgaa
     7561 gccccttttc atgagcctgt gatacataat ctgcacttgc gatgcttgca atgtttcctg
     7621 aagtatctgt cagatagaac caggtaggcg agatcacatt cacacctgtc atatttgctg
     7681 tcgcatccgc aaaataagca ttggcatctg tggaagtcac ctgatgccat accatattga
     7741 ttttgctgtc catagtaaga taggagtact cttccttctt aaagcttcgt tcaaattttg
     7801 tcttctctgc ttctcccact ttcttcttat ctatataacc gatatatccg tcatccgtag
     7861 ctacctgatc ccagttctcc atttcttcaa taagacgaag ctttgtacct ttctttacag
     7921 aagtaagaat agccgatttg attccaccgc ggtaacgtat gcttgtattc ttcttaactg
     7981 tgactgtttt tacatttttg aatttatact gtatggcaat cctgtatgga tcttttgtga
     8041 tgtatgcatc aagatctgta aattcctgca cataagtaag attcagatac actttgtcat
     8101 ctttcaccca taccttatct cctgcctcag atgatgcact cacagaagtc agctccgaag
     8161 gtgttgcata aagtatctgc tgattggcag aatcccagta atatctctgg ttcagataag
     8221 tattcaccac atccagtggt aaatagactc tgtctccgtc caccagacca cgttcttcca
     8281 gtttctcagt tccaatgacc agggcaatct ccccatctgc catttctcca taatattcat
     8341 tcagatccat tttctcagaa gatggtatgt acttcattac tacatgagtg acaattccaa
     8401 gaatcgcaac aagaatgacc aaaacggcaa ctgcgataac tggtttatac ttt
//
LOCUS       DBMDXG010000041         8423 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2259_length_8423_cov_1.860250, whole genome shotgun sequence.
ACCESSION   DBMDXG010000041 DBMDXG010000000
VERSION     DBMDXG010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2259_length_8423_cov_1.860250"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="ACZPD3_02295"
     CDS             complement(<1..902)
                     /locus_tag="ACZPD3_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1414869.1"
                     /translation="MKKPVLVVMAAGMGSRYGGLKQIDPVDKEGHIIMDFSIYDAVQA
                     GFRKVVFIIKKENEADFRSAIGDRLSNQLEVSYVFQDLHNIPEGYEVPEGRVKPWGTG
                     HAVLSCINEIDGPFVVINADDYYGSHAFKMAYDFLTENEDTADTYRYMMVGYKLENTL
                     TENGHVARGVCVTDEEGHLLKINERTHIEKHDGGTAYTEDDGKTWTMLPEGSTVSMNM
                     WGFSASILKELKDRFPRFLDENLKVDPLKCEYFLPFVVDELLGEKRATVKVLKSMDKW
                     YGVTYKEDKPVVVAAIQNLKDGGL"
     gene            complement(1063..1941)
                     /locus_tag="ACZPD3_02300"
     CDS             complement(1063..1941)
                     /locus_tag="ACZPD3_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1414870.1"
                     /translation="MAKEKEARRPFNLKREVKLLPGYIILLLWVIFTIALLGWVFGAS
                     FSTTAEIFQGKALKFESGLHFENYAKAWNGAGVAGFFGNSLIYSVISCTILILVCAPA
                     AYVLSRFDFIANKFIQTSFVSAMGVPAIMVVLPLFSIISGMGILNNVAAGRTVLIILY
                     VGINVPYTTIFLLTFFSNISRTYEEAAAIDGCPPMRTFWKIMFPMAQSGIVTVTIFNF
                     INIWNEYFLSLIFASSDKLKPVAPGLYGMINSMKYTGDWAGMFAAVIIVFLPTFILYI
                     FLSEKIIGGVTGGIKG"
     gene            complement(1955..2860)
                     /locus_tag="ACZPD3_02305"
     CDS             complement(1955..2860)
                     /locus_tag="ACZPD3_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1414871.1"
                     /translation="MKKNKTMIVVFLTPAVLLFLGIFLYPIIRTVIMSFFKIDGVTDS
                     MDLWKFVGFGNYTKLMSTSLFKTSFFNLFRIWLIGGLVVMSLSLLFAVILTSGIRGKK
                     FFRAIIYMPNIVSAVALATMWLQYVYSPRYGLLKDLFTALHLDNLAKIQFLDNNHKFW
                     ALLFAYCFGMVGYHMLIWLSGIERISPEYYEAATIDGATKPAQFRYMTLPLLKGVFKT
                     NITMWSVSSVGFFVWSQLFSTVTADTQTITPYVYMYMQIFGGGNTVTERNAGLGAAIG
                     VLLSVCVVIVFTICNRVIKDDDLEF"
     gene            complement(3021..4265)
                     /locus_tag="ACZPD3_02310"
     CDS             complement(3021..4265)
                     /locus_tag="ACZPD3_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1414872.1"
                     /translation="MKKKMLSATLCAVMVGSLAAPAFTVQAADDTLVYWSMWEATEPQ
                     GQVIQEAVDAYTEQTGVKVDLQFKGRTGNREALQPALDGGTQIDIFDEDIDRVNGMYG
                     KYLLDLEDLAKENDYEATANAGLMAACRDAGGGTLKTIPYQPNVFAFFYNKDLFEQAG
                     ITEEPKTWDEFKDVCQKLKDAGITPMTMDDAYATCVIGYHLARLVGEDKVKEIVTEGE
                     WDDPAVLQMAQDIEELASNGYYSEMVGSNVWPAGQNTELALGTAAMYLNGSWLPNEVK
                     EMAGPDFHWGCFAYPALTDGANGIETNNFGAQVFGINKDTKLAKEAFDLITFLTKGEY
                     DQKLADETVGIPADTTNTEWPAMVECAKPVIEQSTNRFTWACGVESNVDMTPVIKENF
                     IKLMAGSITADEFVSTMQDAAK"
     gene            4528..5427
                     /locus_tag="ACZPD3_02315"
     CDS             4528..5427
                     /locus_tag="ACZPD3_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1414873.1"
                     /translation="MAFESMYLEEDIHIDRIYTIHYFEYKSDFHFAGERHNFWEFQCV
                     DKGKAEIETDNGIYHLTSGQLIFHRPNEFHNLIAIGNTAPNIVVVSFECNSPCMKFFE
                     KKLLKLSDPERNLMGMLIAEARRCIKTPLDDPYTEKMEKKEDFLFGSQQLIRIYLEQM
                     LIYMIRRNSSPPMTVPVSQFVNLKNNSAVYKRVIAYMEDHIRENITLCDLCHDNMIGR
                     SQLQKIFQEQHQCGAMDFFSRLKIAYARQLIRENQMNFTQISEFLGYSSIHYFSRQFK
                     KISGMTPTEYITSIKALSERERQ"
     gene            complement(5468..6736)
                     /locus_tag="ACZPD3_02320"
     CDS             complement(5468..6736)
                     /locus_tag="ACZPD3_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1414874.1"
                     /translation="MEKWRSIAAGMSCLSMLFATGAVTVSASDEITEIPEQSIVYWSM
                     WEEDEPQADVIREAAEAYEEATGISVEIQWKGRGIRSLIEPALDAGEQIDLFDDDYQR
                     MAQEHRDYLAELKGMADTVDYEKHIMPVLLEQVKNWGNGELLAMPYQPYITGVWYNKD
                     LWEEAGLTEQDIPDTWEKLIRVCRKIKNSDSGLSAMTCDEEYVNLLYGYQLARYLGQE
                     KVQQLIHNCTWSQIPQAKEAADDIRILFFAGYMSQSAPAQHPEGQDEVGDGEAVMVLQ
                     GSWVPNEVTEATESDDSWGFFPWPAVKAGTDGTEGVMVGAQGFGVTKDSQMKQEAFDF
                     AYSICTGEIDMKMTDAVNSIPADTDNTQWPEVLADAVPYMKEMSKPYMWAAGLEADPD
                     YKEQIQSELLKLTRLEETSDEFIENLSNMK"
     gene            complement(6930..8273)
                     /locus_tag="ACZPD3_02325"
     CDS             complement(6930..8273)
                     /locus_tag="ACZPD3_02325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414875.1"
                     /translation="MKHKIFSHTSLLIILSVILTFLAAGTVMYNRYDIYMKQGVRDEA
                     AYIKTGLEEDGDVFLSDRVGDATSSRITLLGKDGQVLFDSIENPEEMENHSNRPEFIE
                     AEKQGSGEMVRYSDTLSKQTFYYAVKLKDDQVLRVARTTDSLLVTMLTSFLLLGGLVC
                     VILVIELFLVQKQTRKLIEPINRIDLEHPLEHVCYEELRPLLFRLDQQNRQIQKQLED
                     LKNAESARKEFTANVSHELKTPLMSISGYAELMMNGMVPPDKMQDFSGRIFHESERLS
                     NLVADIIQLSRLDEKNGETMFEQVDIGELGEDVINNLQNRAAKKKINLEYTGGPAQMQ
                     GVRHVLYEMFYNITDNAIRYTPDGGDVKVFVGKLNGKPYFRVEDNGIGIPESEQQRIF
                     ERFYRVDKSHSRETGGTGLGLSIVKHGAVLHHAKILLDSEPGKGTKMEILFDQTK"
     gene            complement(8306..>8423)
                     /locus_tag="ACZPD3_02330"
     CDS             complement(8306..>8423)
                     /locus_tag="ACZPD3_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR1414876.1"
                     /translation="FDGETRTVDVHVRTLRQKLGEAGNLIETVRGVGYRIGA"
BASE COUNT         2222 a         2065 c         1508 g         2628 t
ORIGIN      
        1 tacagccctc catcttttaa attctgaatg gcagctacta ccacaggttt atcttctttg
       61 tatgttacgc cataccattt atccatagat ttcagaacct ttacagttgc tctcttctca
      121 ccgagaagtt catctactac aaatggcagg aaatattcac atttcaacgg gtctactttc
      181 agattctcat ccaggaatct ggggaatctg tctttcagct ctttcaggat acttgcggaa
      241 aatccccaca tattcatgga tactgtactg ccttccggaa gcatggtcca tgtttttcca
      301 tcatcttctg tataagctgt gccgccgtca tgcttttcga tatgggtacg ctcgttgatc
      361 ttaagaagat gtccttcttc gtctgtcacg cacacacctc ttgccacatg gccattctct
      421 gtcaaagtat tttccagttt atagccgacc atcatatatc tgtaagtatc cgcggtatct
      481 tcattctctg tcaggaaatc atacgccatc ttaaatgcat ggcttccgta ataatcatct
      541 gcattgatca caacgaatgg tccgtcgatt tcattgatgc agcttaatac tgcatgtccg
      601 gttccccatg gtttcacacg tccttccggt acttcatagc cttccggaat attgtgaaga
      661 tcctggaaaa catatgatac ttctaactgg ttactgagac ggtcaccgat ggcgcttctg
      721 aagtctgctt cattttcttt tttgataata aacactactt tccgaaatcc ggcctgtact
      781 gcatcataaa tggagaaatc cataatgatg tgtccctctt tgtccaccgg atcgatctgt
      841 ttcagaccgc cgtaacggct tcccatacct gctgccataa ctactaatac tggtttcttc
      901 ataatttttt tcctcttttc tttaaatgtt ttcgttactc tcagattgtc tggtaacttt
      961 tttacattca tcttatcata ctatgatttt gaggtcaaaa cgtgttacat aatacctttc
     1021 acaatcgcaa gaattgtgtt actgccctgc tgtcctctat tattatccct taataccacc
     1081 ggtaacaccg ccgataatct tctctgacag gaaaatatac agaataaatg tcggcaggaa
     1141 tacaatgatc actgctgcaa acattccggc ccagtcccct gtatatttca tggagttgat
     1201 cattccataa agtccgggtg caacaggttt cagcttatcc gagcttgcga agatcaagga
     1261 caggaaatac tcgttccaga tattaataaa gttaaagatt gttactgtta cgattcctga
     1321 ctgcgccatg ggaaacatga ttttccagaa agtacgcatg ggagggcatc cgtcgattgc
     1381 tgccgcttcc tcataggtcc tggaaatatt gctgaaaaat gtaagcagga aaatggtcgt
     1441 ataaggtaca ttaattccta catacaatat gatcagtaca gttcttcctg cagccacatt
     1501 attcaggatt cccataccgg aaataatgct gaaaagggga agaacaacca tgattgccgg
     1561 aacacccatg gcagatacga agctggtctg gataaatttg tttgcaataa aatcaaatct
     1621 ggacagtaca taagctgcag gtgcacacac cagaatcagt attgtgcagg aaattactga
     1681 atagatcaga gagtttccga agaagcctgc aactccggct ccgttccatg cttttgcata
     1741 attttcaaaa tgtaatccgg actcaaactt cagggctttt ccctggaaga tttctgctgt
     1801 tgttgaaaaa cttgccccga atacccatcc cagaagcgca atggtaaaga ttacccagag
     1861 cagcaggatg atgtatcccg gcaggagttt gacttctctt ttcagattaa agggtctgcg
     1921 tgcctctttt tcttttgcca tttctcatcc ctccttagaa ttccagatca tcatctttga
     1981 tcactctgtt acagattgta aatacaatga ctacgcagac actgagcagt acaccgatgg
     2041 cagctcccag acctgcattt cgttctgtaa ctgtattacc gccgccaaaa atctgcatat
     2101 acatataaac ataaggggta attgtctgtg tgtctgcagt tactgtggag aaaagctgtg
     2161 accatacaaa aaagcctaca ctgcttacag accacatggt aatgtttgtc ttaaatactc
     2221 cctttaacaa tggaagtgtc atgtaacgga actgtgccgg tttggtcgct ccgtcaatgg
     2281 ttgccgcctc ataatattca gggctgatac gctcaatacc gctgagccag attagcatat
     2341 ggtatccaac cattccaaaa caatatgcaa acaacagtgc ccaaaatttg tggttattat
     2401 ccagaaactg gatttttgcc agattgtcca gatgcagtgc tgtaaaaaga tctttcagca
     2461 gaccatatct tggactgtat acatactgca gccacatagt agcaagcgct actgcactga
     2521 caatatttgg catataaatg atcgcgcgga aaaatttctt tcctctgatt ccactggtaa
     2581 gaataactgc aaataaaaga gacaggctca ttaccaccag accgccgatc agccagatac
     2641 gaaacaggtt aaaaaatgaa gtcttaaata agctggtact catcagcttt gtatagttgc
     2701 caaatcctac aaatttccat aaatccatgg aatctgtaac tccgtcgatc ttgaaaaagc
     2761 tcatgatcac tgttctgatg atcggataca gaaagattcc cagaaacagc agaactgccg
     2821 gagtcagaaa tacaacgatc atcgttttat tcttcttcaa ataaatacct cctctgtaag
     2881 caatatcata tctacggttt ccaagtctaa aagtcttatc gcgcaagcac gaacgacttt
     2941 ttgcctttga aacccggata ttcaaaaaga cagatggcgg cagcgtagat acgatgccac
     3001 catctctata aattctttat ttatttcgct gcatcctgca tagtggaaac aaattcatct
     3061 gctgtaatgg aacctgccat taatttaata aagttttctt taataaccgg agtcatatct
     3121 acgttagatt ctacaccgca tgcccatgtg aaacggtttg tgctctgctc aataacaggt
     3181 tttgcacact caaccattgc cggccattct gtgtttgttg tatcagccgg gatacctaca
     3241 gtttcatctg caagtttctg gtcgtattca ccttttgtaa ggaatgtgat cagatcaaat
     3301 gcttcttttg caagttttgt atctttgtta ataccaaata cctgtgcacc aaagttgttt
     3361 gtttcgattc cgtttgctcc gtctgtaagt gccggatatg cgaagcatcc ccagtggaag
     3421 tcaggacctg ccatctcttt tacttcgttt ggtaaccagg agccattcag atacattgca
     3481 gctgttccaa gtgcaagctc tgtgttctga cctgcaggcc atacgttgga accaaccatc
     3541 tctgaataat atccgttaga tgcaagttct tcgatatcct gtgccatctg aagaactgca
     3601 ggatcatccc attcaccttc agttacgatc tcttttactt tatcttcgcc aaccagtctt
     3661 gcaagatgat atccgatcac acatgttgca taggcatcat ccatagtcat tggtgtgatt
     3721 cctgcatctt ttaatttctg gcatacatct ttgaattcat cccatgtctt cggttcttct
     3781 gtgattcctg cctgctcaaa cagatctttg ttatagaaga aagcaaatac gtttggctga
     3841 tatggaattg tttttaatgt gccgccgccg gcatcacggc atgctgccat cagacctgcg
     3901 tttgcagttg cttcatagtc attctctttc gcaaggtctt ccaaatccag aagatattta
     3961 ccgtacattc cgtttacacg gtcgatatct tcgtcaaaaa tatcaatctg tgtaccgccg
     4021 tccagtgcag gctgaagtgc ttcacggttt ccggtacgtc ccttaaactg aaggtctacc
     4081 ttaactcctg tctgttctgt ataagcgtca actgcttcct gaatcacctg tccctgtggc
     4141 tctgtagctt cccacattga ccagtaaact aatgtgtcat ctgctgcctg aacagtgaaa
     4201 gccggtgctg caagtgaacc aaccataacc gcacataatg ttgcactaag catctttttc
     4261 ttcataccca taaccttttt cctcctttgt cggttttgac attttgtctt cttctttttt
     4321 ctctcttttc gttcttttgt tgaatttcaa caattatttc ttttggtagt tcttattcta
     4381 tcatttttgc acaaatagta tttgcacaaa cgtccatttc ttttgtacaa aaattcattt
     4441 tcaactggtt tttcatctgt ttttgttgta tacttataca caacaaccat attgcagttt
     4501 tcggacaggc taagggaggt ggcagtaatg gcttttgaga gtatgtatct ggaagaagat
     4561 attcatattg accggattta tacaatccac tattttgaat acaaaagcga ttttcacttt
     4621 gcaggagaac gccataattt ctgggaattt caatgtgtag ataagggaaa ggcagaaata
     4681 gagacagata acggtatcta ccatctgaca agcggacagc tcatctttca cagaccaaat
     4741 gaatttcata atcttattgc cataggaaat accgcaccta atattgtggt agtttctttt
     4801 gaatgcaatt ctccatgtat gaaatttttc gagaaaaaat tgctgaaact ttctgatcct
     4861 gagagaaatc tcatgggaat gctgattgcc gaggcacgcc gctgtatcaa aacccctctg
     4921 gatgaccctt atacagaaaa gatggaaaag aaagaagatt ttctgtttgg ttctcagcag
     4981 cttatccgta tttaccttga gcagatgctg atctacatga ttcggagaaa ttcttctcct
     5041 cctatgactg taccagtcag ccaatttgta aatctgaaaa ataattccgc tgtctataag
     5101 cgtgtcattg cttatatgga agaccacatc cgggaaaata ttacactctg cgatctgtgc
     5161 catgacaata tgatcggacg ctcccagctt cagaaaatat ttcaggaaca gcatcagtgc
     5221 ggcgctatgg atttcttttc ccggttaaag atcgcctatg ccagacagct gatccgcgag
     5281 aaccagatga acttcacaca gatttcggaa tttctgggat actcttccat ccattacttt
     5341 tccagacagt ttaaaaaaat ttccggaatg acacccacag aatatattac atcaataaag
     5401 gcgctctcag aacgagagcg ccagtaatac cagaccaata ttttcataat gttgaacagc
     5461 ctttttctta cttcatattg ctgagattct ctataaattc atccgatgtt tcttctaatc
     5521 ttgtaagctt cagaagttcg ctttgtatct gttctttata atccggatct gcctccagac
     5581 ctgcagccca catgtacggc tttgacatct ctttcatata cggtactgca tctgccagca
     5641 cttccggcca ttgtgtattg tccgtatctg ccgggatgga attaaccgca tctgtcattt
     5701 tcatatctat ttccccggta cagatggaat atgcaaaatc aaaagcttcc tgtttcatct
     5761 gcgaatcctt cgtgactcca aatccctgtg ctcccaccat aacaccttct gtgccgtctg
     5821 ttccggcctt tactgcaggc catggaaaaa atccccagga atcatcagat tctgtagctt
     5881 ctgtcacttc attcggaacc caggagcctt gcagcaccat gactgcctcg ccatctccca
     5941 cttcatcctg accttccgga tgctgtgccg gtgcactctg actcatatat cccgcaaaaa
     6001 acaaaattct gatatcatct gccgcctctt ttgcctgtgg aatctgtgac caggtacagt
     6061 tatggatcag ctgctgcact ttctcctgtc caaggtatct ggcaagctga tatccataca
     6121 gaagattcac atactcttca tcgcaggtca tggcactaag gccactgtca ctgtttttaa
     6181 tcttacggca tactctgatc agtttctccc aggtatccgg gatatcctgt tctgtaagtc
     6241 cagcctcttc ccataaatcc ttattatacc agacgcctgt gatataaggc tgatacggca
     6301 ttgcaagcag ttctccattt ccccagttct tcacctgctc caacagtacc ggcataatat
     6361 gcttctcata atccacagta tctgccattc cttttaattc tgccagataa tccctgtgtt
     6421 cctgagccat cctctgataa tcatcatcaa aaaggtcaat ctgttctccg gcatccaggg
     6481 caggttctat cagagaccgg attcctctgc ccttccactg aatctctaca gaaatacctg
     6541 ttgcttcttc ataagcttct gctgcttctc tgatcacatc tgcctgtggt tcatcttctt
     6601 cccacatgga ccagtataca atactctgtt ccggaatttc tgtgatttca tctgatgctg
     6661 atacagtaac cgctcctgtt gcaaacagca ttgacaaaca gctcataccc gctgcaatgc
     6721 ttctccattt ttccatactg aaaataccct ccattgtacc tgcttttaca tggataactg
     6781 tctgatttta ttttctccaa taatcacttg tctgtcaatc cccaaaattt atacatttct
     6841 aacaaccatg tcggatactg tccactctct tttcaacgcc agacaaaatc atccgcttca
     6901 ctacaccgaa taactgcttc acaggagctt tacttagtct gatcaaacag gatttccatc
     6961 ttcgttccct tacccggctc actgtccaga agaatcttcg catgatgcaa aactgcgcca
     7021 tgttttacaa tagaaagccc caggcctgta cctccggttt cacgtgaatg gctcttgtcc
     7081 actctgtaaa aacgctcgaa gattctctgc tgttcgcttt ccggaattcc tattccgtta
     7141 tcttccacac gaaagtacgg tttcccattg agttttccca caaaaacctt tacatctcca
     7201 ccatccgggg tatagcggat agcattatct gtaatattgt aaaacatttc atataataca
     7261 tggcgtactc cctgcatctg tgcaggtccg cctgtatatt ccagattaat ctttttcttt
     7321 gctgccctgt tctgcagatt gttaatcaca tcttccccaa gctctccgat gtcaacctgt
     7381 tcaaacatag tttctccgtt cttctcatcc agtctggaaa gctgaatgat atctgcaacc
     7441 agatttgata aacgctcgga ctcatgaaaa atcctgccgg aaaaatcctg catcttgtcc
     7501 ggcggtacca ttccattcat cataagctcc gcatatccgg agattgacat aagaggagtt
     7561 ttcaattcat gggaaacatt tgccgtaaac tcttttcttg cgctctctgc attcttaaga
     7621 tcttccagct gtttctgaat ctgtctgttc tgctgatcca gccgaaaaag caatggcctt
     7681 aattcttcgt agcatacatg ctccagagga tgttccagat caatccggtt gataggctct
     7741 atcagctttc tggtctgttt ctgcacaaga aaaagttcaa ttacaaggat cacgcatacc
     7801 agtccgccaa gcagaagaaa gctggttaac attgttacca gaagactgtc tgtagttctg
     7861 gctactcgca gaacctgatc atcctttaat tttacagcat agtaaaacgt ctgttttgat
     7921 aaggtgtctg aatagcgtac catctcaccg gatccctgct tctcagcttc aataaattcc
     7981 ggtctgttac tgtgattttc catttcttcc ggattctcaa tactgtcaaa caacacctga
     8041 ccatcttttc ccagaagagt gatcctgctg ctggtggcgt ctccgaccct gtctgagaga
     8101 aaaacgtctc cgtcttcttc cagacctgtt ttaatatatg cggcttcatc tctgacaccc
     8161 tgtttcatat aaatatcata tctgttatac atgacagttc ctgctgccag gaatgtaagt
     8221 attacggata aaataataag taaactggtg tgactgaata ttttatgctt catttttccc
     8281 ctcctgtttt cgtcggtttt atctgttatg cgccgatcct gtatcctaca ccccggaccg
     8341 tttcgatcag atttcctgcc tctccaagtt tctgacgcag ggttctcaca tggacatcca
     8401 cagttctggt ttcaccgtca aag
//
LOCUS       DBMDXG010000042         8328 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2290_length_8328_cov_1.649082, whole genome shotgun sequence.
ACCESSION   DBMDXG010000042 DBMDXG010000000
VERSION     DBMDXG010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_8328_cov_1.649082"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="ACZPD3_02335"
     CDS             <1..856
                     /locus_tag="ACZPD3_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR1414877.1"
                     /translation="ESVIIFYMMRTLGAKVKMGHCALYSFVGFFFSCITPSASGGQPM
                     QIYFMKKDKLPIPVTTLVLMIVTITYKAVLVVIGIVIWIFGRGFLEGYLGQYMWVFYL
                     GVGLNIFCVTFMMILVFAPGLAKWIMVKGLKLIEHFRFLKPKTTRLEKLEASMDQYHE
                     TAAFWASHKLVILKVLLITMVQRILLFTVTYWVYRSLGFHGYSIITLTILQSVISVSV
                     DMLPLPGGMGISESLYLVMFAPVFGEALLPAMLLSRGISYYAQMLISAVMTCVAYFII
                     GRKEQEEK"
     gene            868..1488
                     /locus_tag="ACZPD3_02340"
     CDS             868..1488
                     /locus_tag="ACZPD3_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR1414878.1"
                     /translation="MFLGIYDYTVILTYISLGISVFGITRALEGDFKVAIFCLALSGL
                     CDMFDGKIARTKKNRTDDEKNFGIQIDSLCDVVCFGIFPAMICYCLGVNTSAGIGALI
                     FYSVASVIRLAYFNVSEAKRQNETSENRQYYQGLPITSMAIILPFLYLMRRYCGLYFL
                     IVIHIAVIIVGLLFILDIKVKKPQNPVRILLVAVVALALAKMFRLI"
     gene            1572..2486
                     /locus_tag="ACZPD3_02345"
     CDS             1572..2486
                     /locus_tag="ACZPD3_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR1414879.1"
                     /translation="MKYIDRKGNITIEENEQDKFLRHLYNDRGGRLCLKLLIRPFVSK
                     AAGVLLNTRLSARFVPDFVKNNKIDLSIYEKQNFSSWNDFFIRRIRKEERPIDMRENI
                     LISPCDGKLSVHRISSDSRFSIKDTEYTAGQLLKNKAIAERYTGGYALIFRLTVDDYH
                     HYCYVADGRKSANVTLPGVFHTVNPAANDVYPIYKENAREYTLLKTKQFGTILMMEVG
                     AMMVGKITNLHKNPATVKKGQEKGNFEFGGSTIILLIQPGKVRIAYDLIENTEEGYET
                     IVKMGERIGECRKLKNTKNHYEGTIENT"
     gene            2556..3185
                     /locus_tag="ACZPD3_02350"
     CDS             2556..3185
                     /locus_tag="ACZPD3_02350"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR1414880.1"
                     /translation="MLNKRNYQKELEKLIDQAQKDNKIPSLFLHSCCAPCSSYVLEYL
                     SKYFNITVFYYNPNIYPEEEYRKRVHEITRLVNSMEFEHPVKLIEGHYDPQEFFQMAK
                     GLEDVPEGGERCFKCYRLRMEEAAKLAEEGGYDYFTTTLSISPLKNAAKINEIGQELA
                     EIYHVQHLPSDFKKKNGYKRSIELSHEYDLYRQNYCGCVYSRREAENRK"
     gene            3551..4930
                     /gene="argH"
                     /locus_tag="ACZPD3_02355"
     CDS             3551..4930
                     /gene="argH"
                     /locus_tag="ACZPD3_02355"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR1414881.1"
                     /translation="MAQLWGGRFTKETDKLVYNFNASISFDQKFYEQDIRGSKAHVAM
                     LARQGILTAEEKDQIEAGLDGILADVRSGKLEITSEYEDIHSFVEANLIDRIGDAGKK
                     LHTGRSRNDQVALDMKLYVRDEIDETDELVKKLLEALQKIMEENVHTYMPGFTHLQKA
                     QPVTLAHHVGAYFEMFVRDRSRLADIRKRMNTCPLGAGALAGTTYPLDREYTAQLLGF
                     DGPTRNSMDSVSDRDYVIELLSAFSTIMMHMSRFCEEIIMWNSNEYRFVEIDDAYSTG
                     SSIMPQKKNPDIAELVRGKTGRVYGALTSILTTMKGIPLAYNKDMQEDKELTFDAIDT
                     VKGCLALFTGMISTMQFNKQNMEASAKNGFTNATDAADYLVNHGVPFRDAHGIVGRLV
                     LTCIDKGISLDELPLEEYKAISPVFENDIYEAISMKTCVEKRMTIGAPGPDVMEKVIA
                     ENKKYLEEN"
     gene            5068..6153
                     /gene="asd"
                     /locus_tag="ACZPD3_02360"
     CDS             5068..6153
                     /gene="asd"
                     /locus_tag="ACZPD3_02360"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR1414882.1"
                     /translation="MSEKLRVGILGATGMVGQRFISLLENHPWFEVVTVAASPRSAGK
                     TYEEAVGGRWKMDTPMPEGVKNLVVMNVNEVEKVAATVDFVFSAVDMSKEEIKKIEEE
                     YAKTETPVVSNNSAHRWTPDVPMVVPEINPEHFDVIESQKKRLGTTRGFIAVKPNCSI
                     QSYAPVLTAWKEFEPYEVVATTYQAISGAGKTFKDWPEMVGNIIPYIGGEEEKSEKEP
                     LRIWGKVEDGVIKPATEPVITCQCIRVPVLNGHTAAVFVKFRKNPTKEQLIKALVEFK
                     GLPQELELPSAPKQFIQYLEEDNRPQVTEDVNFEHGMGVSVGRLREDTVYDWKFVGLS
                     HNTVRGAAGGAVLCAETLKAKGYIQAK"
     gene            6218..6454
                     /locus_tag="ACZPD3_02365"
     CDS             6218..6454
                     /locus_tag="ACZPD3_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HIR1414883.1"
                     /translation="MKEKVLTTVVTFETTTQAIAMERECKKNKFTGRLIPVPREISAS
                     CGLAWKCGEQNEEETASYMKEQDLEYEKIYRILA"
     gene            6701..7738
                     /gene="selD"
                     /locus_tag="ACZPD3_02370"
     CDS             6701..7738
                     /gene="selD"
                     /locus_tag="ACZPD3_02370"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR1414884.1"
                     /translation="MKKNEVRLTQLASAAGCGAKIGPKVLAQVVGKLPKFTDPMLLVG
                     PETSDDAAVYKINDELAMIHTVDFFPPVVDDPYMYGQIAAANALSDVYAMGGVPKLAL
                     NVVAFPNCLGPEVLEQILRGGADKVMEAGAVLAGGHTINDKEPKYGLCVTGYVHPDKM
                     WKNYGAEEGDILILTKPLGCGILNTAIKAEMASQEEIERVQKIMAKLNKYAAEIASKY
                     TIHSCTDVTGFSLAGHSLEMAKGSRKTLVIQSEKLPIIEGVEEYAQMGLIPEGAYRNR
                     DFAGDEVRSEIKELWMEDLVFDPQTSGGLLLAVPAEEADALAEELAGMDIFGGVIGYV
                     TELQDKAVVFE"
     gene            7988..>8328
                     /locus_tag="ACZPD3_02375"
     CDS             7988..>8328
                     /locus_tag="ACZPD3_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR1414885.1"
                     /translation="MNYEDVQKVSNAAKAKSNLVNTNFFKYFIRAVMAGFFIDVAMIY
                     SNVVGNVFSKTMPEWGKFVGALVFSIAVLLISFVGGELFTGNNMVMAFGAYDKQVSWK
                     EAGKVWGVSYLG"
BASE COUNT         2623 a         1630 c         1988 g         2087 t
ORIGIN      
        1 cgagtctgta attattttct atatgatgcg gactctcgga gcaaaagtaa agatggggca
       61 ttgtgctctg tattcttttg tgggattctt tttcagctgt atcacaccgt ctgcatccgg
      121 cggacagcca atgcagattt attttatgaa aaaggacaaa cttccgattc ctgtcactac
      181 gctggtcctg atgattgtca caattactta taaagcggtt ctggtagtga tcggaattgt
      241 aatctggatt tttggcagag gctttctgga aggatatctt ggccagtaca tgtgggtgtt
      301 ttacctgggt gtaggtctga atattttctg cgttactttc atgatgatcc ttgtatttgc
      361 acctggactg gcgaaatgga tcatggtaaa aggtctgaaa cttattgaac atttccgttt
      421 ccttaagcca aagacaaccc ggcttgagaa actggaagcg tctatggacc agtatcatga
      481 aactgcggct ttctgggcaa gtcacaagct ggtgatcctg aaagtgttac tgatcactat
      541 ggtgcagagg attttgttat ttacagttac atactgggta taccgttcgc tgggatttca
      601 tggatattcc attataaccc ttacgatttt gcagtctgtc atatctgtct ctgttgatat
      661 gctgccttta cccggaggaa tgggaatcag tgagtctctt tatctggtga tgtttgcacc
      721 ggttttcgga gaagcattac ttcctgcaat gctgctcagc agaggaattt cctattatgc
      781 acagatgctt atcagtgcag tgatgacctg tgtggcgtat ttcataatag gaagaaaaga
      841 acaagaagaa aagtaggggg agaaaatatg tttttaggaa tttatgatta tacggtaata
      901 ttgacttata taagtctggg aatttccgta tttggaatta ccagggctct ggagggggat
      961 tttaaggttg cgattttctg tctggcgctt tccggactgt gtgatatgtt tgacggaaag
     1021 atagccagaa caaagaagaa ccgtacggat gatgagaaga atttcgggat tcagatcgat
     1081 tccttatgtg atgtggtctg ctttggtatt tttccggcaa tgatctgtta ctgtctgggt
     1141 gtgaatacat cggcggggat tggggcgctt attttttaca gtgtggcatc tgtcatccgc
     1201 cttgcatatt ttaatgtttc agaagcaaag agacagaatg agacttctga gaacagacag
     1261 tattatcagg ggcttccgat cacatccatg gcgatcatcc tgccgttcct ttatctgatg
     1321 agaagatatt gcggactcta tttcctgatt gtgatacata ttgcagtgat tatcgtggga
     1381 ctgcttttta ttctggatat caaggtaaaa aaacctcaga atccggtacg tatcctgctg
     1441 gttgcagtcg tggcacttgc acttgcgaag atgttccgtt tgatataaaa taaatataac
     1501 tgttgactgg ccgcagcggt tcccgggaaa cggggaggct gcggttatac ttttatggag
     1561 ggaagatacg aatgaagtat attgacagaa aaggaaatat cacgattgaa gagaatgagc
     1621 aggataaatt cctgcggcat ttatataatg accggggtgg cagactctgc ctgaaactcc
     1681 tgatcagacc tttcgtgtca aaggcagcgg gagtcctgct taacaccaga ctttctgcca
     1741 gatttgtacc ggattttgta aaaaacaaca agattgacct gagcatttac gaaaaacaga
     1801 acttctcttc atggaatgac ttctttataa ggcgcatccg aaaagaagaa cgcccgatcg
     1861 atatgcgcga aaacatcctg atcagcccct gtgacggaaa actttccgta cacaggatca
     1921 gctcagactc acgtttcagc atcaaagata cagaatatac agcaggacag cttctgaaga
     1981 ataaggcaat tgctgagaga tacacaggcg gctatgcgct gattttcaga ctgacagtag
     2041 atgactatca tcattactgc tatgtggcag atggaagaaa atccgcgaat gtaactctgc
     2101 ccggcgtttt ccacacagtg aatcctgcag caaacgatgt atatcccatc tacaaagaaa
     2161 acgccagaga atacactctg ctgaaaacaa aacaattcgg aaccatcctc atgatggaag
     2221 taggcgccat gatggtagga aaaatcacaa atctccataa aaatcctgcc acagtaaaga
     2281 aaggccagga aaaaggcaac ttcgaattcg gaggttccac aataatcctc ctcatccagc
     2341 cgggaaaagt ccgtattgcc tatgatctga tcgagaatac agaagaggga tatgagacta
     2401 ttgtaaaaat gggcgaacga ataggagagt gcaggaagtt aaaaaataca aagaatcatt
     2461 acgaaggcac aatagagaat acctgaacag gtattcgcat tttattaaaa gataataaaa
     2521 agcatttgtg aagatgcaga aaggatataa taatactgtt gaataaacga aactatcaga
     2581 aagaactgga aaaacttata gatcaggcgc aaaaagataa caagatccca agcctgtttc
     2641 tccacagctg ctgtgcaccc tgcagcagtt acgtactgga atacctgtca aaatatttca
     2701 acatcacagt attctactac aacccaaaca tctacccgga agaagaatat cgcaaacgag
     2761 tccatgaaat tacccgtctt gtaaactcca tggaatttga acaccctgtc aaactgatag
     2821 agggtcacta cgacccacag gaattcttcc agatggcaaa aggcctcgaa gacgtacccg
     2881 aaggtggcga acgctgcttc aaatgctacc gcctccgcat ggaagaagca gcaaaactgg
     2941 cagaagaggg cggctatgac tattttacca ccaccctttc catcagccct ctgaaaaacg
     3001 cagccaagat taacgaaatc ggacaagaac tggcagaaat ctaccacgtc caacacctcc
     3061 catctgactt caagaaaaaa aacggctaca aacgttccat cgaactatcc cacgaatacg
     3121 acctctaccg tcagaactac tgcggttgcg tctattcccg aagagaggct gaaaacagaa
     3181 aataaagcag taaccacaat tatgtaacat atagccaata cccagtgaag aaaaagaagg
     3241 tgtgttgggg cgcatttgag ggttcttgta attcttggta ttcgggaatc tgcgttatga
     3301 gccgaccgtc tcacgtgtct gagcgaagtt tgcaacggag cgagtttgag agggcggctc
     3361 atggttttag cagattcccg aatgccttag aattacaggg ttccgaaact aagcctcaac
     3421 acaccttctt tttcttcacg taccttttga atgattccaa actaactact ttgcaatact
     3481 gaaaaaatat gttacactaa cacgtaaaag caataatacc aaaaataaag aagaaacgag
     3541 gaataacaca atggcacagc tttggggcgg acgtttcaca aaagaaacag acaaacttgt
     3601 atataacttc aatgcatcca tctcttttga ccagaaattc tatgagcagg acatcagagg
     3661 cagcaaagca cacgtagcaa tgctcgccag acagggaatc ctgacagcag aagaaaaaga
     3721 tcagatcgaa gcaggtcttg acggaatcct tgcagatgta agatccggca aactcgagat
     3781 cacatccgaa tatgaagata tccacagctt tgtagaagca aaccttatcg acagaatcgg
     3841 tgatgcaggc aagaaacttc acacaggaag aagccgtaac gaccaggtag cccttgacat
     3901 gaaactctat gtaagagatg agattgacga gacagacgaa ctggtaaaga aacttctcga
     3961 agcacttcag aagatcatgg aagagaatgt ccacacatac atgccaggct tcacacacct
     4021 ccagaaagcg cagccggtca cactggctca ccacgtaggt gcatactttg aaatgtttgt
     4081 acgtgacaga agcagacttg cagatatccg taagagaatg aacacctgcc ctctgggtgc
     4141 aggtgcactg gcaggaacca cctacccact tgacagagaa tatacagcac agcttctcgg
     4201 tttcgacggc ccgaccagaa acagcatgga ctctgtatca gacagagact atgtgattga
     4261 gcttctgtcc gcattctcca caattatgat gcacatgagc cgtttctgcg aagaaatcat
     4321 tatgtggaac tccaatgaat accgcttcgt agagatagac gatgcctaca gcacaggcag
     4381 cagtatcatg cctcagaaaa agaacccgga tatcgcagaa cttgtaagag gaaaaacagg
     4441 tcgtgtatac ggagcattaa catctatcct tacaaccatg aagggaatcc ctcttgcata
     4501 caacaaagat atgcaggaag acaaagaact tacatttgat gccattgaca ctgtaaaagg
     4561 atgtcttgcc ctctttacag gcatgatctc tactatgcag ttcaacaaac agaatatgga
     4621 agcaagtgca aagaatggct ttacaaatgc tacagatgca gccgattatc tggtaaatca
     4681 tggagtacct ttccgcgatg cacacggcat tgtaggccgt ctggtgctta cctgtattga
     4741 taaagggatt tctctggatg aacttcctct tgaagaatat aaagccatca gcccggtatt
     4801 cgagaacgat atttacgaag caatcagtat gaaaacctgt gtggaaaaac gtatgaccat
     4861 cggtgcaccg ggacctgacg tgatggagaa agtgatcgct gagaacaaaa aatatctgga
     4921 agaaaattaa aaaaaatgct tgcaatttat tttggtttgt tatagtatgt acaagaaaga
     4981 cagatgtacg caatataagg acacctggat aaaaatgtcc gcgaaagggt agagacgcgg
     5041 gcagaagata aagaggagaa agatattatg agtgaaaagc ttagagtcgg tattttaggt
     5101 gctacaggta tggtaggtca gagatttatc tctctgcttg agaatcatcc atggtttgaa
     5161 gttgtaactg tagctgcaag tccgagaagt gcaggaaaga catacgaaga ggcagttggc
     5221 ggaagatgga aaatggatac tccaatgccg gagggtgtca agaatcttgt tgttatgaac
     5281 gtaaacgaag tagagaaggt tgctgcaaca gttgatttcg tattctctgc tgtagatatg
     5341 tcaaaagaag agatcaagaa gatcgaagaa gaatatgcta agacagagac accggttgtt
     5401 tccaacaaca gtgctcacag atggacaccg gatgtaccga tggtagtgcc ggaaatcaat
     5461 ccggaacact tcgatgtaat cgaatcccag aagaaacgtc ttggaacaac acgcggattt
     5521 atcgctgtta aaccaaactg ttctatccag agttatgctc ctgtattaac tgcatggaaa
     5581 gagttcgagc catatgaagt agttgctact acatatcagg caatctccgg agcaggtaag
     5641 acatttaagg actggccgga aatggttgga aacatcatcc catacatcgg tggcgaggaa
     5701 gagaagagcg agaaagagcc attaagaatc tggggtaaag tagaagacgg tgtaatcaaa
     5761 cctgctacag aaccggttat cacatgccag tgtatccgtg taccggtact gaacggacat
     5821 acagcagctg tattcgttaa attccgtaag aaccctacta aggagcagct tatcaaagca
     5881 ttagtagagt tcaagggact tccacaggaa ttagaacttc caagtgctcc gaagcagttc
     5941 atccagtatc tggaagagga caaccgtccg caggttacag aggatgtaaa cttcgaacat
     6001 ggcatgggcg tatctgtagg acgtcttcgt gaagacaccg tatatgactg gaaattcgta
     6061 ggactttccc acaatacagt cagaggtgca gcaggcggag cagttctctg tgctgaaacc
     6121 ttaaaagcaa aaggatatat ccaggcgaaa taatttatag aaaataataa aagaggtgcc
     6181 ggtaacggtg cctcttttta tgaggacacg acggattatg aaagaaaaag ttctgacaac
     6241 agtggtgact tttgaaacta ccactcaggc aattgccatg gaaagggaat gtaagaaaaa
     6301 caaatttaca ggcagactga tccctgtgcc aagagaaata tcagcttcct gcggccttgc
     6361 gtggaaatgt ggggaacaga acgaagaaga aacagcttcc tatatgaagg aacaggatct
     6421 ggaatatgaa aagatttata ggattcttgc gtaaaatgga aagatttata ggaacacctg
     6481 atatatggta aacggttcac attttgacgg atatgtgaaa acatgataca atgatagcac
     6541 tgaatctctt gttatagaag atatgtaact attcggcagt ctgtcggctg tgaaagtacg
     6601 ttactgttca ctccgtttac agtaacgaaa gtattgaaca gttacgagga gattctgaat
     6661 atggagatca attggggtga actgacttat gggaaatgaa atgaaaaaaa atgaagtgcg
     6721 tcttacacag cttgccagtg cagccggatg cggtgctaag attggcccga aggttctggc
     6781 acaggtagta ggcaaacttc cgaaatttac agatccgatg cttctggtag gtccggagac
     6841 atcagatgat gcagcagtat acaagattaa tgatgaactg gcaatgatcc atacagtaga
     6901 cttttttccg ccagtagtgg atgatccgta tatgtacggg cagatcgcag cagccaatgc
     6961 actgagtgat gtctatgcaa tgggcggtgt tccgaaactg gcattaaatg tggtagcatt
     7021 tccaaactgc cttggtccgg aagtactgga gcagattctg cgcggcggtg cagataaggt
     7081 catggaagcc ggagcagtcc ttgcaggcgg gcatactatc aatgataaag aaccgaaata
     7141 cggactctgt gtaactggtt atgtgcatcc ggacaaaatg tggaaaaatt acggcgcaga
     7201 agaaggagac atccttattc ttaccaaacc gctgggctgt ggaatcctta acactgccat
     7261 taaggcagaa atggcgtctc aggaagaaat tgagagagtt cagaaaatca tggcgaagct
     7321 gaacaaatat gcagcagaga ttgcatctaa atacacgatc cacagctgca ccgacgtaac
     7381 aggattcagt cttgcaggtc acagtctgga aatggcaaaa ggcagccgga aaaccctggt
     7441 gatccagtct gagaaacttc cgatcattga gggtgttgaa gagtatgcac agatggggct
     7501 gattcctgaa ggtgcataca gaaatcgtga cttcgcaggc gatgaagtac gaagtgagat
     7561 taaggaactc tggatggagg atctggtatt tgatccgcag acatccggcg gacttctgct
     7621 tgcagtaccg gcagaagaag ccgatgccct tgcagaagag cttgcaggta tggatatctt
     7681 cggcggtgtt atcggatatg taacagaatt gcaggataaa gcagttgtat ttgaataaaa
     7741 aaatcagctt tactttcaga cattttctcc tgcgtcagca tgtctgcaga tatctttctt
     7801 gtcctttgta ttctgccgaa ctgtctcgat tgcattagta tatggaattg ctataaaaat
     7861 cttcgaatat aatggaacat aattacaaat ctgatgaata ttcttgaatt gtttgacgat
     7921 ttcggttgtg aaacagatat tcaggtgata taatgttctc aataaaatac attgcggagg
     7981 tgtagttatg aattacgaag atgtacagaa agtgtcaaac gcggcgaaag ccaagagtaa
     8041 cctggtgaat accaattttt tcaagtactt tatacgtgcg gtcatggcgg gattttttat
     8101 tgatgtggct atgatctaca gtaatgtagt tggaaacgta ttctccaaga ccatgcctga
     8161 atggggaaaa tttgtggggg cattagtatt ctccattgca gtgcttctga tcagcttcgt
     8221 aggcggcgag ctttttacag gaaataatat ggtaatggcg ttcggagcct atgataaaca
     8281 ggtaagctgg aaagaggccg gtaaggtatg gggcgtaagc taccttgg
//
LOCUS       DBMDXG010000043         8267 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2307_length_8267_cov_2.282513, whole genome shotgun sequence.
ACCESSION   DBMDXG010000043 DBMDXG010000000
VERSION     DBMDXG010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2307_length_8267_cov_2.282513"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="ACZPD3_02380"
     CDS             complement(<1..1068)
                     /locus_tag="ACZPD3_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414886.1"
                     /translation="MIEVNNLVKRYGNHTAVDHLSFKIEKGKIYGFLGPNGAGKSTTM
                     NMITGYIASTEGTVKIDGHDILEEPEAAKKCIGYLPEQPPVYFDMTVLEYMKFVADLK
                     KIPKDKKTNMIEEIMDMVKISDMRNRLIKNLSKGYRQRVGLAEAIMGYPEVIILDEPT
                     VGLDPKQIIEIRTLIKELKKKHTVILSSHILSEVSAVCDYVLIISHGKLVASDTPENL
                     GKLAEGSNTLEMLIKGEKTQIRQALEGIEGVNSVTMEKDEKQNLWSVKVSTEEQNDIR
                     EKVFYKMSEINSPIYEMKSRKVSLEEIFLELTASDKPISADEVPNTPEDKDTDAGKSN
                     ETNVKQTSAEIPEEQNNDKGGE"
     gene            complement(1302..2024)
                     /gene="rsmG"
                     /locus_tag="ACZPD3_02385"
     CDS             complement(1302..2024)
                     /gene="rsmG"
                     /locus_tag="ACZPD3_02385"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR1414887.1"
                     /translation="MPYNLTTLENGCRELGIELSQKQKNQFIQFYEFLVEKNKVMNLT
                     GITEFEEVLTKHFLDSLACVKAIDMTKVKTIMDIGTGAGFPGVPLKIAFPHLEACLLD
                     SLKKRVNFLEESFELLGLEGIKAIHGRAEEYAKNKEYREKYDLCVSRAVSNLATLSEY
                     CLPYVKIGGTFISYKSGTVQEEAEEAEKAINILGGQVKDITYFKLPDSEIDRSLVIIN
                     KKKSTPGKYPRKAGTPLKEPLS"
     gene            complement(2204..4078)
                     /gene="mnmG"
                     /locus_tag="ACZPD3_02390"
     CDS             complement(2204..4078)
                     /gene="mnmG"
                     /locus_tag="ACZPD3_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR1414888.1"
                     /translation="MRTLVEDYDIVVVGAGHAGCEAALAGARLGLNTVMFTVSVDSIA
                     LMPCNPNVGGSSKGHLVRELDALGGEMGKNIDKTFIQSKMLNCSKGPAVHSLRAQADK
                     QAYSNEMRKTLENTPNLTIKQGEVTKLLVEDGKITGVKTYSGATYNCKAVVLCTGTYL
                     KARCIYGDVSNYTGPNGLQAANYLTDSLKELGIEMFRFKTGTPARIAGNTIDYSKMEE
                     QFGDERVVPFSFSTNPEDVQIEQKSCWLTYTNEKTHEIIRANLDRSPLYSGMIEGTGP
                     RYCPSIEDKVVRFADKNRHQVFIEPEGLYTNEMYIGGMSSSLPEDVQEEMYHSVPGLE
                     HAKIVKNAYAIEYDCINPRQLYPTLEFKKIKGLFSGGQFNGSSGYEEAAAQGLIAGIN
                     AALEVKGQEQLILDRSEAYIGVLIDDLVTKENHEPYRMMTSRAEYRLLLRQDNADLRL
                     RKKGYQAGLISEEDYQKILTKEEQIKTEISRVEHTNIGANKEVQTLLESYNSTPLKSG
                     TTLAELIRRPELSYEAIKPLDKERPELPWDVQEQVDINIKYDGYIRRQLKQVEQFKKL
                     EAKKIPTDIDYEKVGSLRIEARQKLEAYRPISIGQASRISGVSPADISVLLVYLSSTK
                     "
     gene            complement(4149..5522)
                     /gene="mnmE"
                     /locus_tag="ACZPD3_02395"
     CDS             complement(4149..5522)
                     /gene="mnmE"
                     /locus_tag="ACZPD3_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR1414889.1"
                     /translation="METTIAAISTAMSASGIGIIRISGENAMDVISRIYRSKGGKKKI
                     KEVPTHTIHYGYIYDGEELIDEVLVMIMHAPRTFTGEDTVEIDCHGGVYAMQRVLDTV
                     LKNGAEIAEPGEFTKRAFLNGRMDLSQAEAVMDVIQAKNEYALRSSMDQLRGSVQKAI
                     RDIREKLIYHIAYIESALDDPEHISLDGYPQELLEVVDNEQKEVKRLLKTSSDGKMIQ
                     EGIQTVILGKPNAGKSSLLNLLIGENRAIVTDIAGTTRDILEEYITLHGISLKIIDTA
                     GIRETKDIVEKIGVDRAREMAQKADLILYVVDSSVPLDENDEEIMEMLTGKKAIILYN
                     KTDLQPEIQPEILEEKTGHPVIPISAKEEKGITELEEQIKDMFFGGEISFNDEVYITN
                     ARHKAALEEADRSLDLVRNSIEMGMPEDFFSIDLMNAYENLGKILGESVGEDLVNEIF
                     SKFCMGK"
     gene            complement(5800..6732)
                     /gene="jag"
                     /locus_tag="ACZPD3_02400"
     CDS             complement(5800..6732)
                     /gene="jag"
                     /locus_tag="ACZPD3_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR1414890.1"
                     /translation="MEDYIQFSAKTKSEAITKACIELGVSSDQLEIQVISEGSNGFFG
                     IGSKPAVIKVRKIESVSEEEEMKEIVETVKLDSFKEEAPVQEEKKTEAIKPVKKEIKE
                     PKAVSEKPRQPKPVKERAAKEKQPREFREPKEKQVREKTTKPVKPVEILTDPEEIKEV
                     ENRAKVFLRDVFASMNLGEVEITSEYNTTDGSLEVDFEGEDMGILIGKRGQTLDSLQY
                     LTSLVVNKGKSNYIRVKLDTEDYRKRRKETLENLARGIAYKVRKTRKPVILEPMNPYE
                     RRIIHSALQGNKFVETVSEGEEPYRHVVVKLKRN"
     gene            complement(6753..8054)
                     /locus_tag="ACZPD3_02405"
     CDS             complement(6753..8054)
                     /locus_tag="ACZPD3_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR1414891.1"
                     /translation="MNLEMILATKSSTPIIGQLATVMGWIMDGIYRLLNIFGIQNIGL
                     CIVIFSILIYALMTPLQIKQQKFSKLSAIMQPELQKIQKKYKGKNNDTAAMQKMQEET
                     QAVYQKYGVSPTGSCVQLAIQFPILMALYQVIYKIPAYVGSVRDILASAVTSITGVNG
                     YTDILQQFITDNKMTRVQLIMDGSKATSNSVTDFLYALSPSQWKTLAETSQFAGFTDT
                     LNSTAKEISHVQNFFGLNIADQPLTYIKAAFVGGSALLAIVAILIPILAWATQMINLK
                     LMPQAAQQSGDSQQDAMMNSMKTMNMVMPLMSAVFCFTFPVGLGIYWVASAAVRSVQQ
                     VVINKKMDKIQIEDLISENMKKMEKKREKAGLPPQKITNQAHQSAKNINKIEKGSSNT
                     NVETRAKKVEEAYKDAANAKPGSITAKANLVKAFDERNKKK"
     gene            complement(8130..>8267)
                     /gene="yidD"
                     /locus_tag="ACZPD3_02410"
     CDS             complement(8130..>8267)
                     /gene="yidD"
                     /locus_tag="ACZPD3_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR1414892.1"
                     /translation="PYIPTCSQYGLEAIEKYGALKGGLLAVWRILRCNPFSHGGYDPV
                     P"
BASE COUNT         2035 a         1899 c         1450 g         2883 t
ORIGIN      
        1 ctctccccct ttatcattat tctgttcttc cggtatttct gctgatgtct gttttacgtt
       61 tgtctcgttt gatttaccgg catcagtatc tttatcttca ggtgtatttg ggacttcgtc
      121 tgcagatatt ggtttatcgg atgcagtaag ttcaaggaag atttcctcca atgatacttt
      181 cctggacttc atctcataga ttgggctgtt aatctccgac attttgtaga atactttttc
      241 ccgaatgtca ttctgctctt ctgttgaaac tttcacactc cagagattct gtttctcatc
      301 tttttccatt gttacgctgt tgactccctc gattccctcg agagcctgcc tgatctgtgt
      361 tttttcacct ttaataagca tctccagagt gttggatccc tctgcaagtt ttccaagatt
      421 ctcaggagta tcgcttgcta ccagttttcc atgagaaatg atcagcacgt aatcgcatac
      481 cgcgcttact tcagaaagta tatgcgagct gagaataacg gtatgtttct tttttaattc
      541 tttaattaag gttcggattt caataatctg tttagggtca agacctactg tcggttcatc
      601 caggatgatc acctccggat accccataat cgcctcagca agaccaactc tctgtctgta
      661 accttttgac aggttcttga tcagacgatt tctcatgtct gaaatcttta ccatgtccat
      721 gatttcttcg atcatgtttg tttttttatc ttttggaatt ttcttgagat ctgccacaaa
      781 tttcatgtat tccaggacag tcatatcaaa atagaccggc ggctgttcag gcagataacc
      841 gatacatttc tttgccgctt ccggttcttc gaggatatcg tgtccgtcaa ttttgacggt
      901 tccttcggtg gatgcgatgt atccggttat catattcatt gtagtggatt ttcctgcacc
      961 gtttggtccc agaaatccat agattttgcc tttctctatt ttaaaggaaa ggtggtcaac
     1021 tgccgtatgg ttaccatatc gtttgaccag attgtttact tcaatcaatg tgttctcctc
     1081 ctcttaattt ttgccaaaat aaaatataga aaatgtcccc aggtgaaaca gagaatgggg
     1141 gaacccgagg acatctctat aaaaatagtt tatgatgaag ttgtgatgaa attaggataa
     1201 aagtgtgatt attctgtgat gtttgtttga aaaaaatagc tgtcccgaaa tctcggcatt
     1261 ttttgccaga agatttcatg agacaactat acatagattt attatgacaa tggttctttt
     1321 aatggtgttc ctgctttacg cggatacttt ccaggtgtag attttttctt atttataatg
     1381 acaagggatc ggtcaatctc ggagtccggc aatttaaagt atgtgatgtc tttcacctga
     1441 ccaccgagga tattgattgc tttctctgct tcttctgcct cttcctgtac agttcctgat
     1501 ttataggaaa tgaaagttcc tcctatcttt acataaggaa gacaatattc tgacagagtt
     1561 gcaaggtttg atactgccct ggatacacag agatcatatt tctcacgata ctctttattt
     1621 tttgcatact cttctgctct tccatgaatc gccttaatcc cttctaatcc aagaagctca
     1681 aaactttcct caaggaagtt taccctcttt ttcagagaat ccagcagaca tgcctccaga
     1741 tgtggaaaag caattttcaa aggcacacct ggaaatccgg cacctgtacc gatgtccata
     1801 attgttttta cttttgtcat atcaatggct tttacacagg caagactgtc aagaaagtgc
     1861 tttgtaagaa cttcctcaaa ctcagtaata cctgtcagat tcataacctt atttttttct
     1921 accagaaatt cataaaactg gataaactga tttttctgct tctggctcag ctctataccc
     1981 agttctctgc atccattttc caatgtcgtc agattatacg gcatgtatta ttctcctttt
     2041 tctaccttta tttattattt ttgtatattt tacagggttg ttttatgtgc ttttatgatt
     2101 ttatctgtta tttatagtcc gttattttac tcttttatgt actattttaa tattttttgc
     2161 caatttcttg tttttataag cacattaaag cgtattatat taattatttc gtactggaaa
     2221 gatacaccag taaaactgat atatcagcag gagaaactcc tgaaattcgg gaagcctgtc
     2281 cgattgaaat cggtctgtat gcttccagtt tctgacgtgc ttcgattctc agacttccaa
     2341 ctttctcata atcaatatct gtcgggattt tctttgcttc cagtttctta aactgttcta
     2401 cctgttttaa ctgtctgcgg atatatccat catacttgat gttaatgtca acctgttcct
     2461 gtacatccca tggcagctcc ggacgttctt tatcaagtgg tttgattgct tcataagaaa
     2521 gttccggacg gcggatcagt tctgccagag tggttccgga tttcagcggt gtgctgttat
     2581 agctttccag aagggtctga acctctttgt ttgctccgat atttgtatgt tctactctgc
     2641 tgatctctgt ttttatctgt tcttctttcg taaggatttt ctgataatcc tcttcactga
     2701 tgagccctgc ctgatatccc tttttacgaa gtctcagatc cgcattatcc tgtcggagaa
     2761 gtaatctgta ctccgctctg ctggtcatca ttctgtatgg ctcatggttt tcttttgtta
     2821 ccagatcatc aataagaaca ccaatataag cctcagaacg gtcaagaatc agctgttcct
     2881 gacctttaac ttccagagca gcattaattc ctgcgatcag tccctgcgcc gctgcttctt
     2941 cataccctga gcttccgtta aactgaccgc cgctgaacag tcctttgatt ttcttaaatt
     3001 ccagtgtagg atataactga cgtggattaa tacagtcata ttcgatggca tatgcatttt
     3061 ttacgatttt tgcatgttcc agtcccggta cggaatgata catttcttcc tgcacatctt
     3121 ccggcagaga actggacatt ccaccaatat acatctcatt tgtatagaga ccttccggct
     3181 caataaatac ctgatgacgg ttcttatctg caaaacggac tactttatct tcaattgacg
     3241 ggcaataacg cggaccggtt ccttcgatca taccggaata caacggagaa cggtcaaggt
     3301 ttgcacgaat gatctcatga gttttctcat ttgtatatgt cagccagcag gatttctgct
     3361 ctatctgaac atcttccgga ttagttgaga atgaaaatgg tacaactctc tcatctccaa
     3421 actgttcctc cattttgctg tagtcaattg tatttccggc aatacgggca ggtgttcctg
     3481 ttttaaaacg gaacatttca attcccagct ctttcagaga atcagtcaga tagttggctg
     3541 cctgcagtcc gtttggtcct gtataattac ttacatcacc gtaaatacat cttgctttta
     3601 aataagttcc tgtacagagg accactgctt tacagttata agttgcaccg gaatatgttt
     3661 ttacccctgt aatctttccg tcctcgacca gaagttttgt tacttctccc tgtttaatag
     3721 tcagattagg tgtattttca agcgtttttc tcatttcatt gctgtatgcc tgtttgtcag
     3781 cctgcgcgcg taaggagtgc actgcaggtc ctttggaaca gttaagcatt ttggactgga
     3841 taaaggtctt gtcaatattt tttcccattt caccgcccag ggcatcaagt tctcttacca
     3901 gatggccttt ggaactgccg cctacgttcg ggttacatgg cattaatgcg atactgtcta
     3961 cacttacagt aaacatcact gtattcaggc ccaggcgcgc gcccgcaagt gctgcttcac
     4021 atccggcatg accggcaccg actacaacaa tatcataatc ttctactaac gttctcattt
     4081 tgtctccttt tattttacaa ctgttcatcc tattctttca aaaatcgccg aaggcgattt
     4141 tttgattttt atttacccat gcaaaacttg ctaaatatct catttaccag atcttcacca
     4201 acagactctc cgaggatttt acccagattt tcatatgcat tcataagatc tatagaaaag
     4261 aaatcttccg gcattcccat ttcgatactg tttctgacca gatcaaggct tctgtcagcc
     4321 tcttccagtg ctgctttatg acgggcattt gtaatatata cctcatcatt aaaggaaatc
     4381 tctcctccaa aaaacatatc tttaatctgt tcttccagtt ctgtaatgcc tttttcttct
     4441 tttgcagaaa tcggaattac cggatgtcct gttttttctt ccaggatttc cggctggatt
     4501 tccggctgaa gatcagtttt attatacagg ataatcgcct tttttccagt aagcatttcc
     4561 atgatttctt catcattttc gtcaagcgga acagaagaat ccactacata aagaatcaga
     4621 tctgctttct gtgccatttc tctggctctg tctaccccta ttttttccac aatatccttt
     4681 gtttcccgga ttccggctgt atcaataatc ttcagagata ttccatgaag tgtgatatat
     4741 tcctccagaa tgtctctggt cgttcctgca atatctgtca caatagcccg gttttctccg
     4801 atcagaagat ttaacagtga ggattttccg gcatttggtt ttccaaggat aactgtctgg
     4861 attccctcct ggatcatctt tccgtcagaa gaagttttca acaggcgctt cacttctttc
     4921 tgttcattat ccacaacttc cagaagttcc tggggatatc catccagact gatatgttcc
     4981 ggatcatcca gtgccgattc tatatatgca atgtgataaa tcagcttttc tctgatatct
     5041 cggattgctt tctgcacaga acctcttaac tgatccatgg aactgcgaag cgcatattca
     5101 tttttcgcct gtataacatc cataacagcc tctgcctggg atagatccat ccgtccgttc
     5161 aggaaggcac gtttggtaaa ttctcctggt tctgcaattt cggcaccatt tttcagcact
     5221 gtatccaaaa ccctctgcat agcatataca ccaccatgac agtctatttc tactgtatct
     5281 tctcctgtaa aagtcctcgg tgcatgcatg atcattacca gaacctcatc aatcagttcc
     5341 tctccatcat aaatatatcc ataatgaatg gtatgagtcg gaacttcttt tatttttttc
     5401 ttcccgcctt ttgatctgta gatacgggaa atcacatcca tggcattctc gccgctgata
     5461 cggatgattc cgattccgga cgcactcatg gctgtggaaa ttgctgcgat tgttgtttcc
     5521 atttttttct cctcacttgc ctgttctata atagttccgc ccctgcggaa cagtagactg
     5581 ttacaataat tctgtcatct tatatacata acaggaaaat cacactgact actgttattt
     5641 ttttctgcaa gtgtgtaatc tctctggtat atatacaaga ctttccgtaa aataaaaatg
     5701 ttactccagg caaaagtaca cacgcaaagt tacgtgctat tcacgctttc acctagaata
     5761 acattctttt ttcatatctg caatgaagca gtcatattat taatttcttt ttaattttac
     5821 cactacatga cggtaaggct cttcaccctc actgactgtc tcaacgaatt tatttccctg
     5881 aagagcagaa tggataattc ttctctcata aggattcatt ggctcaagaa taaccggctt
     5941 gcgggttttt cttactttat aagcaatacc tcttgcaagg ttctcaagag tttctttacg
     6001 gcgcttgcgg taatcttctg tatcaagttt tacacgaatg taatttgatt tacctttatt
     6061 taccacaaga ctcgtcagat actgaagaga atccagagtc tggcctctct tgccgataag
     6121 gattcccata tcttcgcctt caaagtcaac ttccagactt ccgtccgttg tgttatattc
     6181 agatgtaatc tctacttctc caagattcat ggacgcaaat acatcacgaa ggaatacctt
     6241 tgctctgttt tctacttctt taatctcttc cggatctgta aggatctcaa caggctttac
     6301 cggtttagtc gtcttttctc tgacttgttt ctcttttggc tctctgaatt ctctcggctg
     6361 tttttctttt gcagcccttt cctttacagg ttttggctgt cttggtttct cagaaacagc
     6421 tttcggctct ttgatttctt tctttaccgg ttttatagct tccgttttct tttcttcctg
     6481 aaccggggct tcctctttga aggaatcgag tttcactgtc tctacgatct ccttcatttc
     6541 ttcttcctca gaaaccgact caatcttacg aaccttaatt actgcaggct tgcttccgat
     6601 tccgaaaaat ccgttgctgc cttcacttat tacctgaatc tcaagctggt cactggatac
     6661 gccaagctcg atacatgctt ttgtaattgc ttcactcttt gttttcgctg aaaactggat
     6721 ataatcctcc atgccaaccc ctccgttcct gactacttct tcttattacg ctcatcaaat
     6781 gcttttacca gattggcctt tgcagtaatg ctgccaggtt ttgcattcgc agcgtctttg
     6841 taagcttctt caaccttctt ggctcttgtc tcaacatttg tattgctgct gcctttttca
     6901 atcttattga tattcttggc gctctgatgg gcctgatttg taatcttctg aggtggaagt
     6961 cccgctttct cacgcttctt ttccatcttc ttcatattct cactgatcag gtcttcaatc
     7021 tggatcttat ccatcttttt attgataaca acctgctgca cacttcttac tgcagcactt
     7081 gctacccagt aaataccaag acctacaggg aatgtgaaac agaatactgc tgacatcaac
     7141 ggcattacca tattcattgt cttcatggag ttcatcattg catcctgctg tgaatcgccg
     7201 ctctgctgtg ctgcctgagg catcagtttt aagttgatca tctgtgtagc ccacgcaaga
     7261 atcgggataa gaatcgcaac gatagcaaga agagcagatc cacctacaaa agctgcttta
     7321 atataagtca gtggctgatc agcgatattc agaccaaaga aattctgcac atgtgaaatc
     7381 tcttttgcag tgctgttcag cgtatcagta aatcctgcaa actggcttgt ctcagcaagt
     7441 gttttccact gggaaggtga taatgcatac aaaaagtctg tgactgaatt acttgtagct
     7501 ttacttccat ccataataag ctgaactctt gtcatcttat tatctgtgat aaactgctga
     7561 aggatatctg tatatccgtt tacacctgta atactggtaa ctgcagatgc aaggatatcc
     7621 cttacacttc ctacatatgc cggaatttta taaattacct gatataaggc catcaaaata
     7681 gggaactgga ttgcaagctg tacacagctt cctgtaggag atacgccgta tttctgatat
     7741 acagcctgtg tttcctcctg cattttctgc attgcagcag tatcgttgtt ttttcccttg
     7801 tacttctttt gaattttctg aagttccggc tgcatgattg cactcagctt agagaatttc
     7861 tgctgtttga tctgcagtgg ggtcataaga gcataaataa ggatactgaa gatgacaata
     7921 caaagaccga tattctgaat tccaaagata ttcagcaatc tgtaaatacc atccatgatc
     7981 catcccataa cggtcgcaag ctgaccaatg ataggagtgc tgctctttgt cgctaaaatc
     8041 atttccaagt tcatttcctc ctagaatcca taaatgacag gaggttcctt cgatcgatca
     8101 atattcaatt ttcaagtaca ttcctgtctt tacggtacag ggtcatagcc tccgtgggaa
     8161 aaaggattac agcgaagtat cctccatacc gcaagaagac caccctttaa tgcaccatat
     8221 ttttcgattg cttctaaacc atattgagaa catgtgggaa tataagg
//
LOCUS       DBMDXG010000044         8259 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2310_length_8259_cov_2.597696, whole genome shotgun sequence.
ACCESSION   DBMDXG010000044 DBMDXG010000000
VERSION     DBMDXG010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_8259_cov_2.597696"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            66..464
                     /locus_tag="ACZPD3_02415"
     CDS             66..464
                     /locus_tag="ACZPD3_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414893.1"
                     /translation="MRERIESKHVTKEIKSQMPVIPVEIVKERGCTFCPYYLGNYEKQ
                     PRCMMKTCAWDDENERFHPILRKLIPFYKEKMEKAEEKFLAMKKIYTTLLGMFDDEMK
                     QEELEKDECYGCAYGKCGPCIGICYKSMKA"
     gene            478..876
                     /locus_tag="ACZPD3_02420"
     CDS             478..876
                     /locus_tag="ACZPD3_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1414894.1"
                     /translation="MNTTIISGRLVRNPGYNEVENEKGLHKIAKFVLAVRRNYSDEVS
                     FIPVKGFAKKAEFARDYLMQGTKVMVEGEIVTGNYEDKDTGKKIYTTEVYANRIEFAG
                     AKMVDRPPFPEDAEGFLEIPEGMEEEMPFR"
     gene            890..1282
                     /locus_tag="ACZPD3_02425"
     CDS             890..1282
                     /locus_tag="ACZPD3_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414895.1"
                     /translation="MVSVDQAYMVGHMKNADGSINNKNVVLSAACEAAELFQCMMSVN
                     QKLEDWQRFNHTLEMHLRSVECEKIHDICQAVYGMKHPSNELEGLYLAREDEQICLLY
                     YQVFVGMLEINITSAEVLYKQKEETINE"
     gene            1275..2153
                     /locus_tag="ACZPD3_02430"
     CDS             1275..2153
                     /locus_tag="ACZPD3_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIR1414896.1"
                     /translation="MSKMEYMPVDCLQVPKVLFQIEKYKNLSNTAKILYSLFLDRLKF
                     AVQNGWVDHNGDLFVIYPKSEMKKDLNTTRYGVDQAVQELVKAGKLMRIIPNNGKANH
                     FYISDIYENEKEEKSMMTLDTIMANMPLVEREIIMDKMVKASRDILETIADMGYIDEY
                     ETSLTAMEERGYQDELGEMDIDQIQADGYEFGMDLAFGILEENENNPARVLDIQKYVN
                     RQYESCSKKKFMKVLETLVHAMGNDEGFIGDMYACNKKARNYYLDEMIQSFNFVFDEM
                     CHGTGSAANCDFDVEE"
     gene            2163..2981
                     /locus_tag="ACZPD3_02435"
     CDS             2163..2981
                     /locus_tag="ACZPD3_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIR1414897.1"
                     /translation="MADFEYFRAEESDQFSFFRIPKALFTEKEFASLSTDAKVLYGIL
                     LDRISLSKKNGWIDSDGYVYIIYTIAELQELLRMSHTTVTKLLYELDSVHGIGLIERY
                     RQGNNRPSVIYVKNFVKRIRGKPVRYFASGTPETGNPDCKELEIRIARNWKSGTQKNR
                     SPDCKNLDGSNTKINKTEKNHTDKSKGNTPALEEKFSLNGRFKNVVLSEAELQELMTL
                     FPWDYQKRIDHLSVYMKSSGKEYQNHFATICLWAERDGTREGMDKYEFQEGESL"
     gene            2981..3775
                     /locus_tag="ACZPD3_02440"
     CDS             2981..3775
                     /locus_tag="ACZPD3_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1414898.1"
                     /translation="MENKVTADYVDEEGCLHCGICGKRKQMKVSLMGFEHVVSCLCEC
                     EVKARQELDEKMQREEAQRLLYQRKSVGLRERRFWEWKFENDNGSNQKILIARQYVEN
                     WTDMKRKNVGLLLMGPVGTGKSFFAGCIANALLEQGERVMMTNFSRILNEMTSYQADK
                     NQIIQNLVDYPLLIIDDLGIERNSEFALEQVYNVIDSRYCKMLPLIVTTNLGLNEMKS
                     TDLDTAHQRIYSRILEMCVPIYCGGEDKRKEEGTEKLVQVQNLITG"
     gene            3858..4349
                     /locus_tag="ACZPD3_02445"
     CDS             3858..4349
                     /locus_tag="ACZPD3_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HIR1414899.1"
                     /translation="MQEEVTQKTVTFCIRATKITADLLKKVLVAYLRHQKQKSVEKKA
                     QKNQPKQGKVTVKELAKQNAGMVNIEITDKNIKSFERYARKYGINYALKKDKSKEPPV
                     YLVFFKGRDQDALNAAFREFSQKQIQKANKPSIHKRLAAYRAMMPKKTKDKVKNRHQE
                     QSR"
     gene            4346..6232
                     /locus_tag="ACZPD3_02450"
     CDS             4346..6232
                     /locus_tag="ACZPD3_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HIR1414900.1"
                     /translation="MKKKSGKTLMQKWKNKIGGKLSALDKKKLVLTNIPYALAAFYAD
                     RAFFLYRNSPGEDMGNKLLYAMEHADRIFAGFVLSNNWKDLLAGIVVAVVLKVLVWQK
                     QADAKKLRKGIEYGSARWGTAEDIKPYMSEDPWMNIPLTATEALTMESRPKQPKYARN
                     KNIVVIGGSGSGKTRFFVKPSVMQMNCSMVITDPKGTLIEECGKMLTKGPPKRDKDEN
                     VIKDKFGKVVHEPYVIKVLNTINFSKSLHYNPFAYIRSEKDILKLVTTIIANTKGEGE
                     KSSEDFWVKAEKLLYTALIAFIWYEGDEEEKNLNTLLDLLNESETREEDETYQNPVDM
                     MFQELEERDPQHFAVRQYKKYKMAAGKTAKSILISCGARLAPFDIAELREIMSYDEME
                     LDKIGDRKTALFLIMSDTDTTFNFVIAMLQSQLFNLLCDKADDVYGGRLPVHVRVIAD
                     EFANIGQIPQFDKLIATIRSREISASIILQSQSQLKAMYKDSADTILGNCDTTLFLGG
                     KEKTTLKEMSELLGKETIDLYNTSETRSNQKSFGLNYQKTGKQLMTEDEIAVMDGGKC
                     ILQIRGARPFFSDKYDITKHKNYRLLADENEKNRYKVEKELNPQYTPKSEEEVEVIHV
                     ELSE"
     gene            6354..6569
                     /locus_tag="ACZPD3_02455"
     CDS             6354..6569
                     /locus_tag="ACZPD3_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HIR1414901.1"
                     /translation="MAFFSSAITTLKTLVVAIGAGLGVWGVVNLLEGYGNDNPGAKSQ
                     GIKQLMAGAGIMLLGTTLIPQLATLFS"
     gene            6582..7451
                     /locus_tag="ACZPD3_02460"
     CDS             6582..7451
                     /locus_tag="ACZPD3_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HIR1414902.1"
                     /translation="MNTIIERITEAIKDILIGLIKSCLDNMFTSVNEQVGTIAGQVGQ
                     TPQGWNAGIFNLIQNISQTVIVPIAGLIITFVLCYELITMVTQKNNFHEFETYNIFLW
                     IFKAYVAIYLVTNTFNITMAVFDVGQHVVNNAAGVISGNTAVDASEAIARIVDALEDM
                     ELGDLFLLSMETLLISITMRILSIIITVILYGRMIEIYLYTSIAPIPFATMTNKEWGN
                     IGNNYLKGLFALAFQGFFMMVCVGIYAVLVNAMTISSDLHAAMFSVAAYTVILAFSLF
                     KTGSLSKSIFNAH"
     gene            7475..7939
                     /locus_tag="ACZPD3_02465"
     CDS             7475..7939
                     /locus_tag="ACZPD3_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HIR1414903.1"
                     /translation="MAYVPVPKDLTKVKTKVALNLTKRQLIFFSLAAVVGIPFYLVMR
                     KPIGSSIAAILMVTIMLPFFFMAMYEKDGLPFEKVVANIIRQKFICPEVRPYKTENFY
                     QEISTLVKKEGVPDGKKDKAGGSAGASVPGGKKKPSGKKKQKKQKRKRTGKA"
     gene            8016..>8259
                     /locus_tag="ACZPD3_02470"
     CDS             8016..>8259
                     /locus_tag="ACZPD3_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIR1414904.1"
                     /translation="MLQDGICQVTKTFFSKTIQFYDINYQLALNEDKTTIFENYCDFL
                     NYFDSSISVQLSFINQQVDVAEFEKSIDIPDQNDDFN"
BASE COUNT         2739 a         1372 c         2055 g         2093 t
ORIGIN      
        1 accaaaaatc aggcaattca ggggaaatcc cttttgcaaa tatatatttt tggaggattt
       61 ttaagatgag agagcgaatt gaaagtaagc atgtaacaaa agaaatcaaa agtcagatgc
      121 cagtaatccc ggtggaaatt gttaaagaaa gaggctgtac gttctgcccg tattatctgg
      181 gcaattatga gaaacagcca cgctgtatga tgaaaacctg tgcctgggat gatgaaaatg
      241 aacgtttcca tccaattctt cgtaaactga tcccatttta caaagagaag atggaaaaag
      301 cagaagaaaa attcctagca atgaaaaaga tttacacaac gctgttagga atgtttgacg
      361 atgaaatgaa gcaggaagag ttggaaaaag atgaatgtta cggatgtgcc tatggaaaat
      421 gtggaccatg tatcggtatc tgttacaaat caatgaaagc ataggagggt taagccaatg
      481 aatacaacaa ttatttccgg gcgattggta agaaatcccg gatacaacga agtggagaat
      541 gaaaaaggac ttcataagat tgccaagttt gttctggcag tcagaagaaa ttattctgat
      601 gaagtcagtt tcattccggt aaagggtttt gcaaagaaag cagaatttgc cagagattat
      661 ctgatgcagg gaacgaaggt catggtggaa ggcgagattg tcacgggtaa ttatgaagat
      721 aaagataccg ggaaaaagat ctataccacc gaagtgtatg caaaccggat tgaatttgca
      781 ggtgcaaaaa tggttgacag accaccattt ccagaagatg cggaagggtt tttagagata
      841 ccggagggca tggaagagga aatgccgttt agatagagga ggataaaaga tggtttcagt
      901 agatcaggcg tatatggtag ggcatatgaa aaatgccgat ggaagtatca ataacaaaaa
      961 tgtagtgctg tcagcagcat gtgaagcagc ggaactgttc cagtgcatga tgtcagtaaa
     1021 tcagaaattg gaggactggc agagatttaa ccatactttg gagatgcatc tgagaagtgt
     1081 ggaatgtgag aaaatccatg atatctgtca ggcagtatat ggaatgaaac atccgtccaa
     1141 tgaactggaa ggattgtatc tggcaaggga ggatgaacag atatgcctgc tgtactatca
     1201 ggtatttgtg ggtatgctgg aaatcaatat tacttcagcc gaagtgctgt acaaacagaa
     1261 ggaggaaaca attaatgagt aagatggagt atatgccggt agattgtctg caggtgccaa
     1321 aggtgctgtt ccagatagaa aaatacaaaa atctgtcaaa tacagcaaag attttatata
     1381 gcttatttct ggatcgtttg aagtttgcag tgcagaatgg ctgggtagat cataatgggg
     1441 atttgtttgt aatctatccg aaaagtgaaa tgaaaaaaga cttgaatacg accagatatg
     1501 gagtagatca ggcagtacag gaactggtta aggctggaaa gctgatgcga attattccaa
     1561 acaatggaaa agcgaaccac ttttatatca gcgatattta tgaaaatgag aaggaggaaa
     1621 aatcaatgat gacattagat actattatgg caaatatgcc gttagttgaa agagaaatta
     1681 ttatggataa gatggtgaaa gcatccaggg atattttgga aacaattgca gatatgggat
     1741 atattgatga atacgaaacg tctttaactg caatggaaga gagaggatat caggatgagc
     1801 ttggcgagat ggatattgat caaattcagg ctgatggata tgagtttggc atggatcttg
     1861 cgtttggaat tctggaggaa aatgaaaata atcctgctcg tgtgttagat attcagaaat
     1921 atgtgaacag acagtatgaa agctgttcaa aaaagaagtt tatgaaggtc ctggaaaccc
     1981 tcgttcatgc gatggggaat gatgaaggct ttattggtga tatgtatgct tgcaacaaga
     2041 aggctagaaa ttattatttg gatgaaatga ttcagtcatt taattttgtg tttgatgaga
     2101 tgtgtcatgg tacaggcagt gcagcaaatt gtgatttcga tgtagaagag taggaaaata
     2161 ttatggcaga ttttgaatat ttcagagcag aagaatctga ccagttttct ttttttcgga
     2221 taccaaaagc tctgttcaca gaaaaagagt ttgcaagcct gtctacagac gcaaaggtcc
     2281 tttacggaat tctgcttgat cgaatcagcc tatccaagaa aaatggatgg attgatagtg
     2341 atggttatgt ctatatcatt tacacgatcg cagaattgca ggaactattg cgaatgtcgc
     2401 atacgacagt gacaaagctt ctttatgagc ttgacagtgt gcatgggatt ggattgattg
     2461 aacggtatcg gcaggggaat aatcgtccct ctgtcatata cgtgaaaaat tttgtgaaac
     2521 ggattagagg aaagcccgta agatactttg cttctggaac gccagaaact ggaaatccgg
     2581 actgcaagga attggaaatc cggattgcaa gaaactggaa gtccggaacg caaaaaaata
     2641 gaagtccgga ctgcaagaat ctggatggaa gtaatactaa gataaataag actgaaaaga
     2701 atcatactga taagagtaag ggcaatactc ccgctttgga agaaaaattc agtctaaatg
     2761 ggagatttaa aaatgttgtt ctgtcggaag cagaactgca ggagttgatg acgctatttc
     2821 catgggatta ccagaaacgg atagatcatc tttctgttta tatgaaatcc agtggaaaag
     2881 agtatcagaa tcattttgca acaatctgtc tttgggcaga aagggatgga accagggaag
     2941 gaatggacaa atatgaattt caggaaggag aaagcctgtg atggaaaata aagtaacagc
     3001 agattatgtg gacgaagagg gatgtcttca ttgtggcatc tgtgggaaaa gaaaacagat
     3061 gaaagtcagt ctgatgggat ttgagcatgt agtgtcctgt ttgtgcgaat gcgaggtgaa
     3121 agccagacaa gaacttgatg aaaagatgca gcgggaagaa gcacagcgat tgctttatca
     3181 gagaaaatcc gtcggacttc gggaaagaag attctgggaa tggaaatttg aaaatgacaa
     3241 tggaagcaac cagaagatac taattgccag gcagtatgta gaaaactgga cagatatgaa
     3301 gagaaaaaat gtaggattgc ttttgatggg accagttgga accgggaaaa gtttctttgc
     3361 aggctgtatt gcaaatgcac ttttggaaca gggagaacgt gtcatgatga cgaatttttc
     3421 caggatctta aacgaaatga ccagctatca ggcagataag aatcagatta tacaaaatct
     3481 tgtggattat ccgctgctga ttattgatga tttaggaata gaacgaaatt ccgagtttgc
     3541 tttggagcag gtctataatg tgatagatag ccgttattgt aaaatgcttc cgctgatcgt
     3601 aacaacgaat ctgggactga atgaaatgaa atccacagat ttggatactg cccatcagcg
     3661 tatttacagt agaatacttg aaatgtgtgt tcccatctat tgtggtggag aagataagag
     3721 aaaagaagaa gggacagaaa aactcgtaca ggtgcagaac ctgattaccg gttaatctgt
     3781 cttttttcat taaagggggt gattgccatg attacagatc tggacagcag ttgaatcgct
     3841 gaaaggaggg aaaagtcatg caggaagagg taactcagaa aacggtcacg ttctgtattc
     3901 gggcaactaa gattacagca gacctgttga aaaaggttct tgttgcgtat ctgcgtcatc
     3961 agaagcagaa atcagtagag aagaaagcac agaaaaatca gccgaaacag ggaaaggtca
     4021 cggtaaaaga gctggcaaaa cagaatgccg ggatggtcaa tatcgagatc acggacaaaa
     4081 atatcaagtc ctttgaacgg tatgctagaa aatacgggat caattatgct ttgaaaaaag
     4141 acaagagcaa agagcctccg gtttatctgg tatttttcaa gggacgtgat caggatgccc
     4201 tcaatgctgc tttccgtgag ttttcccaga aacagattca aaaagcgaat aagccttcca
     4261 ttcataagcg tcttgcagct taccgggcaa tgatgccaaa gaagacgaag gacaaagtaa
     4321 agaaccggca tcaggagcag agtcgatgaa aaagaaaagc ggcaaaactc tgatgcagaa
     4381 gtggaagaat aaaatcgggg gtaagctgtc cgccctggat aaaaagaaac tggttcttac
     4441 aaatatccct tatgctctgg ctgctttcta tgcagaccgg gcattttttc tttaccggaa
     4501 cagtccggga gaagatatgg ggaacaaact tttatatgca atggaacacg cagaccggat
     4561 ttttgctggt tttgtgttaa gcaataactg gaaagatctg ctagcaggta ttgttgttgc
     4621 agtcgttctg aaagtgttgg tctggcagaa acaggcagac gcaaagaaat tacggaaagg
     4681 catcgagtat ggttcagcca gatggggaac cgcagaggat atcaagcctt atatgtcgga
     4741 agatccatgg atgaacattc cattgacagc aacagaagca ctgaccatgg agagcagacc
     4801 gaagcaaccg aagtatgcaa gaaataaaaa tatagtggtc atcggcggca gtggttccgg
     4861 taagacacgt tttttcgtaa aaccatcggt catgcagatg aactgttcca tggtcattac
     4921 agatccgaag ggcactctga tcgaggagtg cggaaagatg ttgacaaaag gaccaccaaa
     4981 gagggataaa gatgaaaatg tcatcaaaga taaattcgga aaagtggtgc atgagcctta
     5041 tgtgattaag gttctgaata ccattaactt ttccaaatcc cttcattaca atccgtttgc
     5101 ttatatacgg tctgaaaaag atatcttaaa gctggtgacg acaattattg ccaataccaa
     5161 aggggaaggt gaaaaatctt ccgaagattt ttgggtgaaa gcagagaaat tgctctacac
     5221 agcactgatt gcttttatct ggtatgaagg ggatgaagag gaaaaaaatc tgaataccct
     5281 tctggatctt ttgaatgaaa gcgaaacccg tgaagaggat gaaacttacc agaatccagt
     5341 ggatatgatg ttccaggaac tggaagaaag agatccgcag cactttgcgg tcagacagta
     5401 taaaaaatac aaaatggccg ccggcaaaac cgcaaaatcg attttgattt cctgtggagc
     5461 cagacttgcc ccatttgaca tcgcagagct tcgtgagatc atgtcttatg atgaaatgga
     5521 actggataag atcggtgaca ggaaaacagc cttgtttctg attatgtccg atacagatac
     5581 aaccttcaac tttgtgattg cgatgctgca gtcgcagctt ttcaatctgc tttgcgataa
     5641 ggcagacgat gtatatggtg gaagacttcc ggtgcatgta cgtgtcatcg ctgatgagtt
     5701 tgccaacatc gggcaaattc ctcagtttga caagctgatt gcaactatcc gaagcaggga
     5761 aatctctgct tctattattt tgcagtcaca gagtcagcta aaggcaatgt acaaggatag
     5821 tgcagatacc attctgggta actgtgatac tacactattt cttggtggaa aagagaaaac
     5881 taccttaaag gaaatgagtg aacttctggg taaggaaacc attgatctgt ataacacttc
     5941 ggaaacgagg agcaatcaga agtcctttgg attgaattac cagaaaacag gcaaacagct
     6001 gatgacagaa gatgagattg cagtcatgga tggtggaaaa tgtatcctgc agatccgtgg
     6061 ggccagacca tttttctcgg acaaatatga tatcacgaaa cacaaaaact atcggcttct
     6121 tgcagatgaa aacgagaaga accggtacaa agttgagaaa gagttaaatc cgcagtatac
     6181 accgaaatct gaagaagagg tggaagtaat tcatgtagaa ctgtcggagt aaccttggga
     6241 atgaacagaa aataatgagg aaaggaataa ctgacactag gtgcaccggc tttttagtcg
     6301 gtgtattttc gttcccgtaa acacctgaaa gaagtcagtt ttggtgaatg tatatggcat
     6361 tttttagcag tgcaattaca actttaaaaa ctcttgtagt cgcaatcggc gcaggtcttg
     6421 gcgtgtgggg tgtcgtaaac cttctggaag gatatggaaa tgacaatccc ggcgctaaga
     6481 gccagggcat caaacagctg atggcaggtg ccggaatcat gctgctggga actaccctga
     6541 ttccacagct ggcaacattg ttctcttaag aggagtggta gatgaacacc atcattgaga
     6601 gaattacgga agcgataaag gatatcctga tcgggcttat caagtcctgt ttggataaca
     6661 tgttcacgtc tgtcaatgag caggtgggaa ccatagccgg gcaggtggga cagacaccgc
     6721 agggatggaa tgccggaatc ttcaatctga tccagaacat ttcccaaacg gtgattgtcc
     6781 ccattgcggg actgatcatc acatttgtct tgtgctatga actgattacg atggtaacgc
     6841 agaaaaataa tttccatgaa tttgagactt acaacatttt cctctggatc ttcaaagcgt
     6901 atgtagctat ttatctggtg acaaatacgt ttaatatcac gatggcggtc tttgacgttg
     6961 ggcagcatgt ggtcaacaat gccgccggag tgatttccgg gaacacagca gtggacgcat
     7021 cggaagcaat cgcaaggatt gtggatgcac tggaagacat ggaactgggg gatttgttct
     7081 tgttatccat ggaaacgctg ttgatcagta ttaccatgcg gattctgtca atcattataa
     7141 ccgttatttt gtacggaaga atgatagaga tttaccttta cacttccata gcaccaattc
     7201 cgtttgccac catgaccaat aaggaatggg gtaatatcgg aaacaactat ttaaaaggct
     7261 tgttcgcact ggcatttcag ggattcttca tgatggtctg tgtgggaatt tatgcagttt
     7321 tagtaaatgc aatgaccata tccagtgacc ttcatgcagc aatgttttca gtagcggctt
     7381 ataccgtgat tcttgcattt tcattattca agaccggaag ccttagcaaa tcaatattca
     7441 atgcccatta gcgggcagga aaggagcgca taccatggcg tatgtaccag taccaaaaga
     7501 ccttaccaag gtcaaaacaa aagtagcgtt aaatctgaca aagcggcagc tgattttctt
     7561 ttcacttgcc gcagttgtgg gaattccgtt ttatctggtc atgagaaaac caatcggttc
     7621 cagtatcgca gccatcctga tggtaacgat catgcttccg tttttcttca tggcaatgta
     7681 tgaaaaagac ggtcttccct ttgaaaaagt ggtggcaaat atcattcgac agaagtttat
     7741 ctgtccggag gtaagaccat ataagacaga gaatttttat caggagattt ccacacttgt
     7801 aaaaaaggag ggtgtgccgg atggcaaaaa agacaaagca ggaggctcag caggagcgtc
     7861 tgtccctggc ggaaaaaaga aaccgtcagg aaagaagaaa caaaaaaagc agaaaagaaa
     7921 gagaacagga aaagcgtagg aagaagcagg aacgggaccg caggaaacag aaaaacattc
     7981 ctacaacagc acagcagagt attccgtata ttcggatgct gcaggatggt atctgccagg
     8041 tgacaaagac ctttttttcc aagacgattc agttttatga catcaactat cagcttgctt
     8101 taaatgagga taagaccacg atatttgaga attattgtga tttcttaaat tactttgatt
     8161 cctccatcag tgtgcagctg tcctttatca atcagcaggt ggatgtggct gaatttgaga
     8221 aaagcattga cattccggat cagaacgatg acttcaatg
//
LOCUS       DBMDXG010000045         8179 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2331_length_8179_cov_2.426219, whole genome shotgun sequence.
ACCESSION   DBMDXG010000045 DBMDXG010000000
VERSION     DBMDXG010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_8179_cov_2.426219"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="ACZPD3_02475"
     CDS             complement(<1..853)
                     /locus_tag="ACZPD3_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR1414905.1"
                     /translation="MRNLYLVRHGKPQYPDEHSYCVGQTDFSLSMLGHLQAVLLNEEL
                     SDKISGVYCSPLLRTVETAGHMAPELPHIIVSDLSERNLGEWDGLSFDEIRQRWPDIY
                     KARGNNPDHPIPGAETPAASGFRFSQAVHKILCASEGDIAVVTHTDVISSYLHALHSD
                     MYSRQQFRLPCGSYYHLEVNEKNNISFSDPSYILPHPELNDGLCLRLRNAVSLPRHVQ
                     AHSDAVTELACCLCNMLESNGYIFDQKLVRSGALLHDIARLQRHHAKTGGELFLQLGY
                     PEISQIIS"
     gene            complement(846..1775)
                     /locus_tag="ACZPD3_02480"
     CDS             complement(846..1775)
                     /locus_tag="ACZPD3_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR1414906.1"
                     /translation="MQTGALIVAAGKSSRMVDFKPMLQLGSISIAQRVINNFRQAGIS
                     KVVVVTGYHADVLECHLASNNVVFLRNENYANTHMFDSVRIGLEYLKDKVDTVLFTPV
                     DVPLFTAQTVTQMLSLGRPLVTPVCNGNPGHPILIRSTLIDSILSDDGKSGLKGAVDN
                     CGEPMYYLNVKDPGIIHDADTPEDYAELLRIHNQSLIRSEIHIQLAREKVFFDEKLYS
                     LLTLIHETGSVRDACERMHISYSTSWNLIHTLESQLHEPLIIRSQGGTRGSHSELTPY
                     GEEFLKRYARFSEETRSCSKKIFEECFGGFLNA"
     gene            1986..2246
                     /locus_tag="ACZPD3_02485"
     CDS             1986..2246
                     /locus_tag="ACZPD3_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414907.1"
                     /translation="MDKIFEITAKEVTVQVKDERTGVVYSRTLPIDYYENANVLKLSG
                     ENLDGSSSSIVFYSARGIERLKDLTGRGADHDSCGTHKPEDQ"
     gene            2283..5045
                     /locus_tag="ACZPD3_02490"
     CDS             2283..5045
                     /locus_tag="ACZPD3_02490"
                     /EC_number="1.2.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent aldehyde oxidoreductase"
                     /protein_id="HIR1414908.1"
                     /translation="MIKKTLKINGVERILILDKDETLAQVLRDHLLLTGCKIGCGEGH
                     CGACNVIMNGKVTRSCIYKMSRVPDHAEITTIEGVGTLSDMHPIQVAWMAYGCAQCGF
                     CSPGFIISAKVLLDNNPSPTREEVRDWFNKQRNLCRCTGYKPLIDATMAAAAVMRGEM
                     TKEDLVFKQTGDSIVGTNYIRPSAAQKVTGTWDFGADDALKMPSGTLRLALTQAKVSH
                     ANILNIDTAEAESMPGVVRVITAKDIKAAGGTNKINGLVMLPKHNKTDGFERPVLCDE
                     KIFQFGDAIAIVAADTEEHARAAAEAVKVEIEELPAYMNAMDAIAPDAAEIHPGIPNA
                     FFETNCIKGPDFDWDSIPDSQQVEIESYCSRQPHLHLEPDCGYGYIDEDGMITVHSKS
                     IGIHLHMPMIADGIGVPMENLRIVQNHAGGTFGYKFSPTNEALIGAAVKILERPVSLV
                     FNQFQNITYTGKRSPAFMNIKLAADENGKLLALWGNNYVDHGPYSEFGDLLTMRLSQF
                     TGAGYGINSIRNKSTTVFTNHAWGSAFRAYGSPQSYMGSEIAIDVLAAKMGIDPFDIR
                     EMNCYKESEGSTIPTGYPPEVYCEEELFKTARPLYEAAKKRVAEKNAASDGKIKYGIG
                     VSLGVYGCGLDGVDTSNAFAELNPDGTVTMYAAWEDHGQGADMGVLVSSHETLRQAGI
                     RPEQIKLVMNDTKTCPNAGPAGGSRSQVMSGNACRLAAENLVAAMKKEDGTFRTYDEM
                     VAEGLATKYEGNWVTTFCADHPIDQDTAQGDPFATYMYTIFLPEVAVNTETGKVKVEK
                     FTCVADVGTIMNRLLVEGNFYGGLVQGIGLALTEDFEDLNHDTSLKNCGIPYPNDAPD
                     DIELHFNETYRPSGPYGAAGCGEAPLDAPHPAILNAIYNATGARITRVPARPEKVLAA
                     LKELEK"
     gene            5129..7423
                     /locus_tag="ACZPD3_02495"
     CDS             5129..7423
                     /locus_tag="ACZPD3_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide
                     oxidoreductase/dicluster-binding protein"
                     /protein_id="HIR1414909.1"
                     /translation="MTYIQERGSTHVYHVNRMSKEEMDHMISLCVHEQPAYCVAACPF
                     KADTKEMLFYAAKGNFKKALAIYEKITPFPMILCNGCTAPCEEKCRLCELGDGISIRE
                     VERAIVRYGEPGKRSSVFRIRKKKKAVIFGSGLFPLFLAGELEKKMYPATIYCQEKDY
                     EAYIAAAAPELLESDRKNEVKRLSSMDLSFEFGCSLDLPFIRAKMKEADVVCASEEVA
                     KKLAPEETADAEIMLREQAGIVSGPVRSVMDAAFAAKRAALTVDLLVQNLSPHSNRGS
                     EGAVTTRLYTNMDGMKGSKKIPCSTDGYSKEEAIEEAKRCIQCHCDECMKSCVYLREY
                     KKHPGLLAREIYNNTQIIMGDHQMNKPMNSCSLCGQCTVTCPNGFDMSQVCKSARENM
                     VSTDKMPLAPHEFALMDMLFSNSEAFLCRPQPGYETCRYVFFPGCQAGAIAPDVVTEA
                     YEDLCRRTEGGVALMLGCCGAISEWAGRYEMTEKVNEQLKQELAKLGDPMIIAGCPSC
                     MKQLKESLGAKVTGIWEILKEIGLPGQAKGLEIPVAIHDACGARGDTQTQDTIRELLA
                     DMGCTVVNTEYSRDRSPCCGYGGLTAYANKEMADKMTEKCLERSDCPYITYCMACRDR
                     FVREGRESRHILELLYGINAANMPDISEKRYNRLELKEKLLKNIWNEELMMEKKDYTV
                     AYTEDAISMMDERMILKSDVERVLSDYRENQEAIFDEETKELVTRSRLGNVTFWVRFV
                     ETEEGYLVRRAYSHRMNIMKRVGQ"
     gene            7423..7644
                     /locus_tag="ACZPD3_02500"
     CDS             7423..7644
                     /locus_tag="ACZPD3_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVU_1557 family redox protein"
                     /protein_id="HIR1414910.1"
                     /translation="MAVERTYYTIDPEGKLTCMKCKVPLVKGKAKFMYLENGFPVEMP
                     VCPKCGFVYVPEELALGKVLAVERALEDK"
     gene            7645..8178
                     /locus_tag="ACZPD3_02505"
     CDS             7645..8178
                     /locus_tag="ACZPD3_02505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1414911.1"
                     /translation="MNRHPGREEQTIHLLKSIELKKGMKALDLGAGEGETVRIMKAFG
                     LNVQGVDLVPRSSEVQQGDFLDLQYAADSMDLCISQCAFFVSRDQKKAVSECWRVLKK
                     GGFLLLSDLDPGNLLEIVKETGFTILCQEDQTALWREYYLEAIWNDSFCCEDHKLLQK
                     EYKGRKIGYTMVVGRKE"
BASE COUNT         2451 a         1579 c         2038 g         2111 t
ORIGIN      
        1 gtgaaatgat ctggctgatc tcaggataac ctaactgtaa gaaaagttca ccgcctgttt
       61 tcgcgtgatg cctctgaagt ctggcaatat catgcagaag tgcaccggag cgaacaagct
      121 tctggtcaaa gatataacca ttggattcca gcatattgca taaacaacag gcaagttcag
      181 taacagcatc actgtgagct tgtacatgac ggggcagaga aacagcattg cgaagcctga
      241 gacatagtcc gtcatttaat tcaggatgag gcagtatata actgggatca gagaaagaaa
      301 tattattttt ttcattcact tccagatgat agtaagagcc gcagggcaga cgaaactgct
      361 gcctgctgta catatcagaa tgtaatgcat gtaaataaga agagattaca tctgtatgag
      421 ttaccactgc aatatcacct tcagaagcgc aaagaatctt atgaactgcc tgcgaaaagc
      481 gaaaaccaga tgcagcagga gtttctgctc ccgggatcgg atgatcaggg ttattcccac
      541 gcgctttata aatgtccggc catctctggc gtatttcatc aaaagaaagg ccatcccatt
      601 ctcccagatt tcgttctgac agatcagaaa caatgatatg tggaagctca ggtgccatat
      661 ggcctgctgt ctcaacagtt cttaacaaag gactgcagta tacccctgat attttatcgg
      721 acaattcttc attaagaagg actgcctgca gatgtccaag catggataga gagaaatctg
      781 tctgtcctac acagtaagaa tgttcatccg gatactgtgg ttttccatgc cggaccagat
      841 aaaggttacg catttaaaaa ccctccgaaa cattcttcaa atattttttt tgaacaggaa
      901 cgtgtttctt ctgaaaaacg ggcatatcgt ttcagaaatt cttcgccgta tggtgtcagt
      961 tcgctatggc ttccccttgt accgccctgg cttctgatga tcagaggttc atgaagctgt
     1021 gattccaggg tatggatcag gttccagctg gtagaatagg aaatgtgcat gcgctcacag
     1081 gcatcgcgga cagatcctgt ttcatggatc agtgtaagca gcgaatagag tttttcatca
     1141 aaaaaaactt tttcccgggc tagctgaata tggatttcag aacggatcag actctggttg
     1201 tgtatgcgga gaagttcagc ataatcctct ggtgtatcag catcatgaat aataccagga
     1261 tcctttacat taagataata catgggttcc ccgcagttat ccacagcacc tttaagtcct
     1321 gattttccgt catcacttag gatggaatca atcagtgtgg aacggatcag aatgggatga
     1381 ccgggattgc cattacagac cggagttaca aggggacgcc ccagagagag catttgagtg
     1441 actgtctggg ctgtgaacag cggaacatca acaggtgtaa agagcaccgt atccactttg
     1501 tctttcagat actcaagtcc gattcggaca gaatcaaaca tatgagtgtt tgcatagttc
     1561 tcatttcgaa gaaagactac attattggaa gccaggtggc attccagaac atctgcatga
     1621 tatccggtta ccactactac tttggagatt ccggcctgcc ggaaattatt gatcacgcgc
     1681 tgtgcgatgg aaatggagcc aagctgaagc atgggtttaa agtctaccat tcgtgatgat
     1741 ttccccgcag ccacgatgag ggcgcctgtc tgcatttaga tacctccttc tttcaatgat
     1801 attatacaaa tttgatatta ttataacata taaaagaata aaaatagtta aaattttaaa
     1861 taaaacaaaa taaaattgtc tgcaatatat aaaatagcca actattaatt atttgtaatt
     1921 tgtataattg tgcaaaatta tggtagtatg gatatataaa ataacgtgga caggagaact
     1981 tttttatgga taagattttt gagattaccg caaaagaagt tactgtacag gtaaaagatg
     2041 agcgtacagg ggttgtatat agccggacgc ttcccatcga ttattatgag aatgcaaatg
     2101 ttttaaagct cagcggtgag aatctggacg ggagcagctc ttccattgtt ttctattccg
     2161 ccagaggaat agagagactg aaagatctga caggcagggg agctgaccat gattcgtgcg
     2221 gaacacataa gcctgaagat cagtaagatt gaaatgaatt tttttaaaag gaggaatggg
     2281 acatgattaa aaagacattg aagattaatg gtgttgagag aatacttatt cttgataagg
     2341 atgagacact ggcgcaggtg ctccgtgatc accttcttct tacaggatgt aagattggct
     2401 gcggtgaagg tcactgtgga gcctgtaatg ttattatgaa cggaaaagtc acaagatcct
     2461 gtatctacaa gatgagccgt gttcccgatc atgcagagat cacaaccatt gaaggtgtgg
     2521 gaacactttc tgatatgcat ccaatccagg ttgcatggat ggcatatgga tgcgcacagt
     2581 gcggtttctg ttcaccggga tttattattt cagcaaaagt gcttctggac aataatccaa
     2641 gtcctacccg tgaggaggta agagactggt ttaacaaaca gcgtaatctc tgccgttgta
     2701 caggctataa gccgctgatc gatgctacta tggctgcagc agcagttatg cgcggcgaga
     2761 tgacaaagga agaccttgtg ttcaaacaga caggtgattc tattgttgga acaaattata
     2821 tccgtccgtc agcagcacag aaggtaacag gtacctggga ttttggtgca gatgatgcat
     2881 taaagatgcc atcaggaaca cttcgccttg ctcttacaca ggcaaaggtt tctcatgcaa
     2941 atattctcaa tattgacaca gcagaagcag agagtatgcc tggtgttgtt cgtgttatca
     3001 cagcaaaaga tattaaggca gcaggcggaa ccaataagat caatggtctt gtaatgcttc
     3061 caaaacacaa caagacagac ggtttcgaac gtcctgtact ttgcgatgaa aaaatcttcc
     3121 agtttggtga tgcgattgcc atcgtagctg ctgatacaga agagcatgca agggcagcag
     3181 cagaagcagt taaagttgag atcgaagagc ttcctgcata tatgaatgca atggatgcca
     3241 tcgcaccgga tgcggcagag attcatcccg gtattccgaa cgcattcttt gagacaaact
     3301 gtatcaaggg tcctgatttt gactgggaca gcattccgga ttcacagcag gttgagatcg
     3361 aatcttactg ttcacgtcag cctcatcttc atcttgagcc ggactgcggt tatggttaca
     3421 ttgacgaaga cggtatgata actgttcatt ccaaatccat tggtatccat ctgcatatgc
     3481 ctatgatcgc tgatggtatc ggtgtgccaa tggagaactt acgaattgtt caaaaccatg
     3541 caggaggaac ctttggttac aagttctctc ctacaaatga ggctctgatc ggcgctgctg
     3601 ttaagatcct tgaacgtcct gtgtctctgg tatttaatca gttccagaat attacttata
     3661 caggaaagag atcccctgca tttatgaata tcaagcttgc agcagatgaa aatggtaagc
     3721 ttcttgcact ctggggcaat aactatgtgg atcatggtcc atactcagaa ttcggtgatc
     3781 tgcttacaat gagactttcc cagtttacag gtgcaggcta tggaatcaat tctatccgta
     3841 acaaatctac aacagtgttt acaaaccatg catggggttc agcattccgt gcttacggat
     3901 ctcctcagtc ttatatggga tctgagattg cgatagatgt tctggctgca aagatgggaa
     3961 tcgacccctt cgatatccgt gaaatgaact gttacaaaga atcagaaggc agtacgatcc
     4021 ctacaggtta tccgcctgaa gtatactgtg aagaagaact gttcaagaca gctcgtccac
     4081 tgtatgaagc agctaagaaa cgtgtggctg aaaagaatgc agcttctgat ggcaagatca
     4141 aatatggtat cggtgtatct ctcggtgtat atggctgtgg tcttgatggt gttgatacat
     4201 caaacgcatt tgcagagctg aatccggatg gaacagttac aatgtatgct gcatgggaag
     4261 atcacggaca gggcgctgat atgggggttc ttgtatcatc acatgaaaca cttcgtcagg
     4321 caggaatcag accggaacag atcaaacttg ttatgaatga taccaagaca tgtcctaatg
     4381 caggtcctgc aggtggatca cgttcacagg ttatgtcagg taatgcatgt cgtctggcag
     4441 cagaaaacct tgtggcagcc atgaagaaag aagacggaac cttccgtact tatgatgaga
     4501 tggtggctga gggtcttgca acaaaatatg aaggtaactg ggtgacaacc ttctgtgcag
     4561 atcatccgat cgatcaggat acagcacagg gtgatccgtt tgcaacctac atgtatacga
     4621 tcttccttcc ggaagttgct gttaatacag aaacaggtaa agttaaagtt gaaaaattca
     4681 cctgtgtggc tgacgtaggt accatcatga acagacttct tgtagaaggc aacttctacg
     4741 gcggccttgt acagggtatt ggtcttgctc ttacagaaga tttcgaggat ctgaaccatg
     4801 atacttctct gaagaactgt ggcattccgt atccaaatga tgcgccggat gatattgaac
     4861 ttcatttcaa tgaaacatat cgcccaagcg gtccttacgg tgctgccggc tgtggtgagg
     4921 ctccgctgga tgctcctcat cctgcaatcc tgaatgcgat ctacaatgct acaggcgcac
     4981 gtatcacacg tgttcctgcc cgtcctgaaa aggttcttgc agcattaaag gaacttgaga
     5041 aataactgtc agtataattt tatcatgtga tataataagg ggagacggga gactgtctcc
     5101 cctttggaaa tgaaaggagt atcgggttat gacatatata caggaaagag gttccactca
     5161 tgtttatcac gtaaatcgaa tgtcaaagga agagatggat catatgatca gcctctgtgt
     5221 tcatgaacag ccggcgtact gtgttgcagc ctgtcctttc aaagcagata caaaagaaat
     5281 gctgttttat gctgcaaaag gaaactttaa gaaagctctg gctatctatg aaaagatcac
     5341 accatttccc atgatcctct gtaacggatg tactgcacca tgtgaggaaa aatgcagact
     5401 ctgtgagctg ggtgatggca tatccatacg tgaagtagag cgtgccattg tacgatatgg
     5461 ggaacctgga aaacggagca gtgtatttcg tatcagaaag aaaaaaaagg ctgtgatctt
     5521 tggctcagga ttgttcccgc tgtttcttgc aggggaactg gaaaagaaga tgtatcctgc
     5581 aaccatttac tgtcaggaga aagattatga agcctatatt gcagcagcag ctccggagct
     5641 tttggaatca gatcgcaaaa atgaggtaaa acgtctcagt tccatggacc tttcctttga
     5701 attcggatgc agtcttgacc ttccgtttat cagagcgaaa atgaaggagg cagatgtggt
     5761 ctgtgcttca gaggaggtgg ccaaaaagct ggctccagag gaaacagcag acgcagagat
     5821 catgctgaga gaacaggcgg gaattgtcag cggtccggtc cggtctgtga tggatgcagc
     5881 ttttgctgca aagagagctg ccctgactgt agacctcctt gtacagaatc tttcgcccca
     5941 cagcaacaga ggcagtgaag gagcagttac caccaggctc tatacaaata tggacgggat
     6001 gaaaggctcc aaaaagattc cctgcagcac agatggatat tcaaaagaag aagctattga
     6061 ggaagcaaag cgatgcattc agtgtcactg tgatgagtgt atgaaaagct gtgtctattt
     6121 aagggaatat aaaaagcatc cgggtcttct tgcacgtgag atttataata atacccagat
     6181 cattatggga gatcaccaga tgaataaacc gatgaattcc tgttcactct gcggacagtg
     6241 tactgtaacc tgtccaaatg ggtttgatat gagccaggtc tgcaaatctg caagagaaaa
     6301 catggtatct acagacaaaa tgcctctggc acctcatgaa tttgcactga tggatatgct
     6361 gttctccaat tcggaggctt tcttatgcag acctcagccg ggatatgaaa cctgcagata
     6421 tgtatttttc ccaggatgtc aggcaggagc tatagcgccg gatgttgtca cagaggcata
     6481 tgaggatctg tgcaggagga cagagggcgg tgttgctctg atgctgggat gctgtggtgc
     6541 tatcagtgaa tgggcaggac gctatgaaat gacagaaaag gtaaatgaac agttgaagca
     6601 ggagcttgca aaactgggag atcctatgat tatagcaggc tgtccaagct gtatgaaaca
     6661 gttaaaagag agtcttggcg ccaaagtcac aggaatctgg gagatcttaa aggaaatcgg
     6721 acttcccgga caggcaaaag gactggagat acctgttgca atacatgatg cctgcggtgc
     6781 caggggagat acacagacac aggatacaat aagagagctt cttgcagata tggggtgcac
     6841 tgttgtaaat acagaatatt ccagagatcg gtctccctgc tgcggatacg gcggactgac
     6901 tgcctacgct aataaagaga tggcagataa aatgacagaa aagtgtctgg aacgatccga
     6961 ttgcccatat ataacgtact gcatggcatg cagggaccga tttgtcaggg aaggcaggga
     7021 atccagacat atcctggaac ttctgtatgg tataaatgcc gccaatatgc cggatatcag
     7081 tgagaaacgc tacaaccggc ttgagctaaa agagaagctt ttaaaaaata tatggaatga
     7141 ggaactgatg atggagaaaa aagattatac cgttgcatat acagaagatg ccatcagtat
     7201 gatggatgaa cgtatgatcc tgaaaagcga tgtggagcgt gtattatcag attacagaga
     7261 aaatcaggaa gcgatctttg atgaagagac aaaagaactg gtgacaagga gcagacttgg
     7321 aaatgttaca ttctgggtga gatttgtgga aacagaagaa gggtatctgg tacgcagagc
     7381 atacagtcat agaatgaata ttatgaaaag ggtgggacag taatggcagt agagagaaca
     7441 tattatacca ttgatcctga gggaaagctg acctgcatga aatgcaaggt tcctcttgta
     7501 aagggaaaag caaaatttat gtatctggag aacggttttc ctgttgagat gccggtctgt
     7561 cccaaatgtg gctttgtcta tgttccggaa gaactggctc ttggaaaggt tttggcagtg
     7621 gaacgtgcac tggaggataa atagatgaac agacatcccg gcagggaaga gcagacaatt
     7681 catcttctga aaagcataga actgaaaaaa ggaatgaaag ctctggatct gggagcagga
     7741 gagggagaga ccgtgcggat catgaaagca tttggtctga atgtgcaggg tgtggatctt
     7801 gttccgcgaa gcagtgaggt gcagcaggga gattttctgg accttcagta tgcagcagac
     7861 agtatggatc tctgtatcag ccagtgtgca ttttttgtca gcagagatca gaaaaaggca
     7921 gtctctgaat gctggcgtgt actgaaaaag ggaggatttc ttttgctgtc agatctggat
     7981 ccgggcaatc ttctggaaat tgtgaaagaa acagggttta cgattctctg tcaggaggat
     8041 cagactgctc tctggagaga atattatctg gaagcaatct ggaatgattc attctgctgc
     8101 gaggatcata agcttctgca gaaagaatac aaaggcagaa aaataggcta taccatggtg
     8161 gttggcagaa aggagtaat
//
LOCUS       DBMDXG010000046         8103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2353_length_8103_cov_1.564359, whole genome shotgun sequence.
ACCESSION   DBMDXG010000046 DBMDXG010000000
VERSION     DBMDXG010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2353_length_8103_cov_1.564359"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            252..1352
                     /locus_tag="ACZPD3_02510"
     CDS             252..1352
                     /locus_tag="ACZPD3_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016777.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1414912.1"
                     /translation="MGTANQIEQELMNYISDHKIRNTHSHHLPEQAMVDFDLDKLINN
                     TYLQWQQVTPGTTAESRNAYLEKTRYKSYFVWLQKAIGELYGISEPITAQNWDQISDQ
                     IRKAHQNPDFYMDVLKNKCKYQKVIVDTYWNPGSDNGRPELFTPAFRLDLFFLGYKKG
                     LRNHDGVSLEENFGELPDNLQDYVEWVRKWIIQKKSEGCVALKIAMAYERSLHFEKVT
                     REQAERVFRLKESDITQEDIRCFQDYLFWKICEIAAEVSLPLQCHTGMGQVIDTNILQ
                     LNNVIKNNPETKFVLLHCGFPWVDDLFSIVDGYPNLYPDLTWLPILSYTASKRVMHQL
                     IEMSQIDKICWGRYVDSRGKLWLPSCIPFFTV"
     misc_binding    1529..1762
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1883..4537
                     /locus_tag="ACZPD3_02515"
     CDS             1883..4537
                     /locus_tag="ACZPD3_02515"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR1414913.1"
                     /translation="MSKELAKTYDPKGIEERLYTKWMDNGYFHAKVNPDKKPFTIVMP
                     PPNVTGQLHMGHALDETMQDILIRFKRMQGYEALWQPGTDHAAIATEVKVIDKLKKEG
                     IDKHDIGREEFLKHAWAWKEEYGGKIINQLKKLGASADWERERFTMDEGCSKAVQEVF
                     IKLYEKGYIYKGSRIINWCPVCQTSISDAEVEHEDQDGFFWHINYPIVGEEGRFVEIA
                     TTRPETLLGDTAVAVNPEDERYKDLIGKMLKLPLTDREIPVIADEYVDKEFGTGCVKI
                     TPAHDPNDFEVGRRHNLEEINIMNDDATINELGGKYAGMDRYEARKAMVADLEKLGLL
                     VKVVPHNHSVGTHDRCGTTVEPMIKPQWFVKMDEMAKAAIKALDDGDLKFVPSRFDKT
                     YLHWLENIRDWCISRQLWWGHRIPAYYCDECGEVVVAREMPEKCPKCGCTHLHQDEDT
                     LDTWFSSALWPFSTLGWPEKTPELEYFYPTDVLVTGYDIIFFWVIRMVFSALEQTDQV
                     PFHHVLIHGLVRDSQGRKMSKSLGNGIDPLEVIDKYGADALRLTLMTGNAPGNDMRFY
                     WEKVEASRNFANKIWNASRFIMMNLEGKTVTEPENLNDLCAEDKWILSHLNTVIREAT
                     ENMEKYELGIAVQKVYDFLWDELCDWYIEMAKVRLWKAEEDPKAANDALWTLRTALTE
                     GLKLLHPYMPFITEEIYCTLLPEEESIMISEWPVYREERNFPDAEKAIEGFKEVVRGI
                     RNTRTEMNVPNNRKTSLHIVAKDAETAAMYENSKKSFVNLAFAKEILVQTDKNGISED
                     AVSVVVSNAVVYMPLEDLIDKDKEIERLTKETERLTKEIARCEGMLNNPNFVNKAPAA
                     KVDAEKDKLAKYKEMMDKVKGQLEQLKK"
     gene            4550..6586
                     /locus_tag="ACZPD3_02520"
     CDS             4550..6586
                     /locus_tag="ACZPD3_02520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR1414914.1"
                     /translation="MKLYKICNKISLLLHVLASAAGYFVMEAICRHSFIEAWNYMTQR
                     PLVFAYNAAFIFTSSLIVYLFHRRVFWRVLVTLFWLILAIINGVLLLNRVTPFTGPDL
                     HLITDAMKIANKYLPVAGVVAVCILFGILVILLLMLLIKGPKYQKKIKYRYNIPLILL
                     AVALFAGSTQLALEKRVLSNYFGNIAFAYEDYGYPYCLATTIFNTGISCPRDYSEKEI
                     KRIEKTEKNLPETQEEKRPNILFLQLESFFDPTLVNYLDISEDPIPTFRKLMKEYSSG
                     YYKVPSVGAGTANTEFESITGMSMHYFGPGEYPYKSILRETTCESAPYVLKNLGYTTH
                     AVHNNEANFYGRRSIFPNLGFDTFTSEEYMARENEKNPNGWVKDEVLTDEILKCLDST
                     EGPDYVYTISVQGHGAYPDEQILEDPEITVSGAPTEEENNKWEYYVNEIHEMDNFVKE
                     LTDKLADYPEDVVLVMYGDHLPTMGLTVEDLKNKYLFQTEYVMWDNFGLKKKNENLAA
                     YQMAAEVMDRVGIHEGTVFRYHQARRNTRNYQVDLETLQYDLLYGKRYSYGESGESPY
                     LRTRMRMGIYDVTLDSIQCISEADHTYYIKGTEFTPSSEIKLNGEWYDTVYINPTTLM
                     ITGTELNDFDRLAVIQRSNSSTRKPLSKSYDRSCYALYSNNKWKLTESAGTNEN"
     gene            6697..7995
                     /locus_tag="ACZPD3_02525"
     CDS             6697..7995
                     /locus_tag="ACZPD3_02525"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1414915.1"
                     /translation="MNYEEAVAYIDETPKFTKKNSLDHTKECLRRLGNPQDKFKVIHV
                     AGTNGKGSTCAFLTSVLREAGYSCGLFTSPHLVEINERFQINEVNIDNDTFLKAFEKV
                     KVLADELVAEGSYHPTYFEMLFLMGMVIFAEAGVDYVTLETGLGGRMDATTAVENPVA
                     CVITSISLDHMQYLGDTVAKIAGEKAGIIVPGVPVIYDGNDPDAAEVIRARAEELGSP
                     AYEVKRSDTEVLRNTSAGIDFAFRNEYYKDMVFSIPFIAKYQVMNSALALKTIEVLQN
                     VISVSMDQIHVGIAGTRWQGRMETVLPGVIVDGAHNEDGVEKFVETAEHFQKECPLTL
                     LFSAVDDKDYTDMIHTICSRITFRQVVVTSVGGYRQVPAERFAKLFTEAGASSVEVVE
                     DVEKAFGRALEVKGDDGMLFCVGSLYLVGEIKDVIRRNKK"
BASE COUNT         2485 a         1440 c         2050 g         2128 t
ORIGIN      
        1 ggttatgttc ggaagaaata ttaatcctgt cagtttcctg tactgcggac tgctgacaat
       61 tggattctcc atgatcgtaa atctgtttat gcatcagaaa ttaaagaaga ttgacatggt
      121 agaatccctg aaaagtgtgg aataaacgat ttgtcgcttt attgcggaaa agaagaaatt
      181 gtttttctgt ggtttttaaa taggtagtca aacggataga atggataaga ggaagaaagg
      241 ggaatattat catgggaaca gcaaatcaga tagagcagga attaatgaac tatatatccg
      301 atcataaaat caggaacacg cattcacatc atctgccgga acaggcgatg gtggattttg
      361 atctggataa gctgataaat aatacgtatt tacaatggca gcaggtaact ccggggacaa
      421 cagcagaaag caggaatgca tatctggaaa aaacacgata taaatcttat tttgtctggc
      481 ttcaaaaggc aatcggggag ttgtatggaa tatcagagcc gatcacagct cagaactggg
      541 atcagatttc agatcagatc agaaaagcgc atcaaaatcc cgacttttac atggatgtac
      601 tgaaaaataa gtgtaagtat caaaaagtaa tcgtagatac ctactggaat ccgggctctg
      661 acaatggaag accggaactg tttacgcctg cttttcgact ggatttgttc tttttgggct
      721 ataaaaaagg acttcgcaat catgacggtg tatcactgga agagaacttc ggtgagctgc
      781 cggacaattt acaggattat gtggagtggg tcagaaagtg gatcatacaa aaaaagagtg
      841 agggctgtgt ggccttaaaa atagcgatgg cctatgagcg tagtcttcat tttgaaaaag
      901 tgacgcggga gcaggctgaa agggtattcc gtttaaaaga gtcggatata acccaggaag
      961 atatcagatg ttttcaggac tatttgttct ggaaaatatg tgaaattgcg gcggaagtgt
     1021 cacttccatt acaatgtcac acaggaatgg ggcaagttat agatacgaat atactgcagt
     1081 tgaacaatgt gataaaaaat aatccggaaa cgaaatttgt gctactgcat tgtggctttc
     1141 catgggtgga tgatcttttt tccatagtag acggatatcc gaatctatat ccggatctga
     1201 catggcttcc aatcttatcc tacaccgcat caaaacgtgt aatgcatcag ttgatcgaga
     1261 tgtcccagat tgataaaatc tgctggggaa gatacgtgga cagtagagga aagttatggc
     1321 tcccttcttg cattccgttt ttcactgtgt agtgttctga gagaaaaaat agaggacgga
     1381 tatctttccg taaataatgc aaaggatatt attgataaaa ttttgttcga caatgcggga
     1441 aaaatatacg tatgacatca gataaatttg tgaaatccgg agaaacattc ttgacaagaa
     1501 agcctttttt cattatagta gaatccagaa aaaggctgtg aaaaagagga gtaggcggta
     1561 atgtcccaca gagagggaag cttataagct ggaaggtttc ccggaaacag aagcgctgaa
     1621 ggtagctttg gagccgggag gctgaagtga gtggtatgat aatactgaga ttcatatacg
     1681 cgcatggtag gctttcacgt atcccgcgtt aagggaatga gagacaaact aaggtggtac
     1741 cgcgatttat atcgcccttt gcacgtataa tgcgtgtaaa gggcgttttt taatcgaata
     1801 agcttataga gctgtgggct gctttacagc ggagtgatcc gcagatactt ttattacaga
     1861 gatagaatat caggaggaca ttatgagcaa agaacttgca aaaacttacg atcctaaggg
     1921 aatcgaagag cgtctttaca caaaatggat ggacaatgga tatttccatg caaaagtaaa
     1981 tccggacaag aaaccgttta ctattgttat gccgccgcca aacgtaactg gccagcttca
     2041 tatgggacat gcgctggatg aaactatgca ggatatcctg atccgtttca agagaatgca
     2101 gggctatgaa gcattatggc agccgggaac tgaccatgca gctatcgcta cagaggttaa
     2161 ggttatcgac aagctgaaaa aagaaggcat cgacaaacac gatatcggcc gtgaggaatt
     2221 cttaaaacat gcgtgggcat ggaaagagga gtacggcgga aagatcatca atcagttaaa
     2281 gaaactgggt gcttccgcag actgggagag agaacgtttc acaatggatg agggatgttc
     2341 caaagcagta caggaagtat ttatcaaatt atatgaaaaa ggctatatct acaaaggttc
     2401 cagaatcatc aactggtgtc ctgtatgcca gacttccatc tctgatgcag aggttgagca
     2461 tgaggatcag gacggatttt tctggcacat taattacccg atcgtagggg aagagggacg
     2521 tttcgtagag atcgcaacta cacgtccgga aaccctgctg ggtgatactg ctgttgccgt
     2581 aaatccggaa gatgagcgct acaaggatct catcggcaag atgctgaagc ttcctctcac
     2641 agacagagag attccggtga tcgccgatga atatgtagac aaagaattcg gaacaggatg
     2701 tgtaaagatc acacctgctc atgaccctaa cgactttgag gttggacgcc gtcacaatct
     2761 ggaagagatc aacattatga atgacgatgc gactatcaac gagcttggtg gaaaatacgc
     2821 tggtatggac cgttacgaag cacgtaaggc aatggtagca gatttggaaa aacttggact
     2881 tctggtaaaa gtagttcctc acaaccacag cgttggaact catgaccgct gcggcacaac
     2941 ggtagagcct atgatcaagc cacagtggtt tgtaaagatg gacgagatgg caaaggctgc
     3001 gatcaaagct ctggatgacg gagatctgaa attcgttccg tcccgttttg acaagacgta
     3061 tcttcactgg cttgagaata tccgcgactg gtgtatttcc agacagttat ggtggggaca
     3121 cagaattccg gcttattact gtgatgagtg cggtgaagta gttgttgcaa gagaaatgcc
     3181 tgagaaatgc ccgaaatgcg gatgtacaca tcttcatcag gatgaggata cactggatac
     3241 ctggttcagt tctgcattat ggccattctc cacacttggc tggccggaga aaacaccgga
     3301 actggaatat ttctatccga cagatgtact tgttacagga tatgacatta tcttcttctg
     3361 ggttatccgt atggtattct ctgctctgga gcagactgat caggttccgt tccatcatgt
     3421 actgatccat ggtctggtac gtgactccca gggacgtaag atgagtaagt ctcttggaaa
     3481 cggtatcgat cctctggagg ttattgataa atacggcgca gatgctcttc gtcttactct
     3541 gatgacaggt aatgcacctg gaaatgatat gcgtttctac tgggaaaagg tagaagcaag
     3601 ccgtaacttc gcaaacaaga tctggaatgc ttcccgtttc atcatgatga acctggaagg
     3661 caagacagtg acagagccgg agaacttaaa tgatctctgt gcagaggata aatggattct
     3721 gtctcactta aatacagtga tccgcgaagc tacagagaac atggaaaaat atgaacttgg
     3781 tattgcagta cagaaggttt atgacttcct gtgggatgaa ctctgcgact ggtatattga
     3841 gatggctaag gttcgtctgt ggaaggcaga ggaagatccg aaggcagcaa atgatgcact
     3901 ctggacatta agaacagcgc tgacagaagg cctgaagctt cttcatccgt atatgccgtt
     3961 tatcacagag gaaatctact gcacacttct tcctgaagaa gagtctatta tgatctccga
     4021 atggcctgta tacagagaag agagaaactt cccggatgct gagaaagcaa tcgagggctt
     4081 caaagaggtt gtaagaggta tccgtaatac cagaacagag atgaatgttc cgaataaccg
     4141 caagacaagc ctgcatattg tggcaaagga tgctgaaaca gcagcaatgt atgagaacag
     4201 caagaaatcc tttgtaaatc ttgcatttgc aaaagagatt ctggtacaga cagacaagaa
     4261 cggaatcagt gaagatgcag tttccgtagt tgtttccaat gcggttgtat atatgccgtt
     4321 ggaagacctg attgacaaag ataaagagat tgaacgtctt acaaaagaaa cagaacgtct
     4381 cacaaaagag atcgcaagat gtgaaggtat gctgaataat ccgaacttcg taaacaaggc
     4441 acctgctgcc aaggtggatg cggagaaaga caaacttgcg aaatataaag aaatgatgga
     4501 taaggttaaa ggacagttag agcagcttaa gaaataaggg agtgtttaga tgaaattgta
     4561 taagatttgt aacaaaatct cccttttact ccatgtactt gccagcgcag cgggatattt
     4621 tgttatggag gcaatctgcc gtcactcgtt tatagaagcc tggaattata tgacacagag
     4681 acctctggtt tttgcatata atgcggcatt catttttacc tcgtcactaa ttgtttatct
     4741 gtttcacagg agagtattct ggagagtact ggttacgttg ttctggctga tactggccat
     4801 tattaacggt gttctcctgc tgaaccgtgt tacaccgttt accggaccgg atctgcatct
     4861 gatcacagat gcaatgaaga ttgccaacaa atatctgcct gttgcaggcg ttgtggcagt
     4921 ctgcatcctg tttgggattc ttgttatttt actgctgatg ctgcttatta aaggaccgaa
     4981 gtatcagaaa aagattaaat atcgctacaa tattcctctg atcctgctgg ctgttgctct
     5041 ttttgcagga agtacacaac tggcactgga gaagagggta ctttccaatt actttggaaa
     5101 tattgctttt gcatatgagg attatggata tccttactgt ctggcaacta cgatttttaa
     5161 tacgggaatc agctgtccga gagattattc tgagaaagaa atcaaaagaa ttgagaagac
     5221 ggaaaagaat cttccggaaa cgcaggaaga aaagcggccg aatattctgt ttttgcagtt
     5281 agaatcattc tttgatccta cgctggttaa ttacctggat atttcagaag acccgattcc
     5341 gaccttccgt aagctgatga aggaatattc ttcaggatat tataaggttc cgtctgtcgg
     5401 tgcaggtact gccaatacgg aatttgaatc cattacagga atgagtatgc attattttgg
     5461 acccggggaa tatccttata agagtattct gagagagact acctgtgaga gtgcacctta
     5521 tgtactgaag aatctgggct ataccaccca tgcggttcat aataatgagg caaacttcta
     5581 tggaagaaga tctattttcc cgaatctcgg atttgatact tttacttctg aagagtacat
     5641 ggcaagagag aacgagaaga atccaaatgg ctgggtaaag gatgaggttc tgacagatga
     5701 aatcctgaaa tgtctggatt ctactgaggg gccggattat gtatatacta tttccgtaca
     5761 gggacatggt gcatatccgg atgagcagat tctggaagat cctgagatca cagtttccgg
     5821 tgcaccgact gaggaagaga acaataaatg ggaatattac gtaaatgaga tccatgagat
     5881 ggataatttt gtcaaagagc tgacagacaa gcttgcagat tatcctgagg atgtggtcct
     5941 tgttatgtac ggtgaccacc ttccgactat gggactgact gtggaggacc tgaagaacaa
     6001 gtatctgttc cagacagagt atgtgatgtg ggataacttc ggacttaaga aaaagaatga
     6061 aaatcttgca gcataccaga tggcggcaga ggttatggat cgtgtgggaa tccatgaggg
     6121 aactgttttc cgctatcatc aggcaagaag aaataccaga aattatcagg tggatctgga
     6181 aaccctgcag tatgacctcc tttatggaaa gagatattcc tacggggaaa gcggcgaaag
     6241 tccttatctg cgtaccagaa tgcgtatggg aatctatgat gtgactctgg attccattca
     6301 atgtatctct gaggcagatc atacatatta cattaaggga acggaattta cgccgtccag
     6361 tgaaatcaag ctgaacggag aatggtatga tacggtctat atcaatccga ctacgttgat
     6421 gatcacaggt acggagctga atgatttcga caggcttgcg gtgatccagc gaagcaacag
     6481 ttcgacgcga aaaccgctga gcaagagcta tgaccggtcc tgctatgcac tttattcaaa
     6541 taataaatgg aaattaacag aaagtgccgg tacgaatgaa aattaatcca taattttgtc
     6601 ggataaaata ttacgattgg tactggacat cttccaccac tattatataa tgttagagta
     6661 actaaataca ttatagataa gtggtggaat ttttttatga attacgaaga agcagttgct
     6721 tatattgatg aaactccgaa atttacaaag aagaacagtc tggatcacac aaaggaatgt
     6781 ctgagaagac ttggtaatcc tcaggataag tttaaggtga tccatgtggc aggaactaat
     6841 ggaaagggaa gtacatgtgc gtttctgacc tctgttctga gagaggcggg atattcctgc
     6901 ggattattta cttctcctca tctggtggag atcaatgaac gcttccagat caatgaggtg
     6961 aatattgata atgatacttt cctgaaggcc tttgagaaag taaaggtact tgcggatgaa
     7021 ctggttgcag aaggcagcta tcatccgact tattttgaga tgctttttct gatgggaatg
     7081 gtgatctttg cagaggcagg tgttgattat gtgactctgg aaacaggact gggcggaaga
     7141 atggatgcga ctactgctgt ggagaatcct gttgcctgtg tgatcacatc gatcagtctg
     7201 gatcatatgc agtatctggg agatacagtt gccaaaatcg caggagaaaa ggccgggatc
     7261 attgtgccgg gggtaccggt aatctatgac ggaaacgacc cggatgcagc agaagtgatc
     7321 cgtgccagag cagaagaact gggaagccct gcttatgaag tgaagagatc agatacagaa
     7381 gtgctcagaa atacttcggc gggaattgat tttgcatttc ggaatgagta ctataaggat
     7441 atggttttct ccattccgtt tattgcaaag tatcaggtga tgaacagtgc acttgcatta
     7501 aagacgattg aggttctgca gaatgtgatt tctgtatcta tggatcagat ccatgtggga
     7561 attgccggaa cacgctggca gggaagaatg gaaacagtgc ttccgggcgt gatcgtggac
     7621 ggtgctcaca atgaggacgg ggtagagaaa tttgtagaga cagcagaaca ttttcagaaa
     7681 gaatgtccgc tgactttgct tttttcagca gtagatgaca aagattatac agatatgata
     7741 catacgatct gcagcaggat cacgttccgt caagtggtag taacctctgt tggcggatat
     7801 cgccaggttc ctgctgagag atttgcaaag ctttttacag aagcaggtgc ttcttctgtg
     7861 gaagtagtgg aggatgttga gaaagccttt ggtagagcac tggaagtaaa gggggatgac
     7921 ggaatgctct tctgcgtggg ttccctgtat cttgtcggtg agattaaaga tgtgatcagg
     7981 agaaataaga aatgatagat tatgaagagg aactgaagaa atttgagccc tgtctggatg
     8041 tgacagacgc ggaagaggct atttacgata gagagctgac ggatattctg gacattcttc
     8101 agg
//
LOCUS       DBMDXG010000047         8007 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2382_length_8007_cov_1.723375, whole genome shotgun sequence.
ACCESSION   DBMDXG010000047 DBMDXG010000000
VERSION     DBMDXG010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2382_length_8007_cov_1.723375"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="tig"
                     /locus_tag="ACZPD3_02530"
     CDS             complement(<1..1028)
                     /gene="tig"
                     /locus_tag="ACZPD3_02530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1414916.1"
                     /translation="MSLQVEKMEKNMAKLTIEVAAEDLEKAMQNAYQKAKGRISIPGF
                     RKGKAPRKMIEQMYGKGVFLEDAVNALIPEHYSKALAECELEIVSQPTIDITQAEPGK
                     AFIFTAEVAVKPEVTLGDYKGVEVPKTEITVTDEDVEAELKKEQEKNSRTISVEDRAA
                     QLNDIVTIDFEGSVDGVPFDGGQATEYPLTLGSNTFIPGFEEQLVGAKVGDDVDVKVT
                     FPEEYQAKELAGKEAIFKCAVKKIEAKELPELDDDFAKDVSEFDTLAEYKEHVKTNLE
                     DKKADEAKRAKEDAAVDKAIENAQMDIPEAMLMTQCRQMLDDFSRRMQSQGLSMDQYF
                     QFTGMTAD"
     gene            complement(1303..1863)
                     /locus_tag="ACZPD3_02535"
     CDS             complement(1303..1863)
                     /locus_tag="ACZPD3_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HIR1414917.1"
                     /translation="MSKKVYIFLADGFEDIEGLTVVDLLRRAGIDIKTVSIKETTRIQ
                     TSHGITMLTDAIFAETDFADADMLVLPGGMPGTKYLAGYKPLIDLLTDFNNKGKKIAA
                     ICAAPSVFSGLGFLKGRKATSYPSFMEVLSKDGAVTSEDSVVVDGNITTSRGLGTAVD
                     FALSLISQLENEEKAKEIAESVVYTR"
     gene            complement(2227..3723)
                     /locus_tag="ACZPD3_02540"
     CDS             complement(2227..3723)
                     /locus_tag="ACZPD3_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1414918.1"
                     /translation="MDEYIKEISEKIEDKKEYYAKISDKIWEYAEPRFQEYKSSELLQ
                     QTLKKEGFSIKSNLAGEETAFIAEYGSGKPVIGFLGEFDALPGLSQKADTTERIPEEK
                     TSDSKYESVPEREKKQNPDSGHTDNCGHGCGHQLIGTGTLASVIALKDFMKEHNLKGT
                     IRYYGCPAEENAGGKAFLVRDGYFDDCDLALCWHPEQGRRACYGSTKANFRVFFTFHG
                     TPAHASMCPELGRSALDAVELMDVGVNYMREHMIDEARIHYAITDTGGDAPNVVQSRA
                     QVLYAIRAPKITQVKELYNRVCNIAKGAALMTETTVEIRQVAAYSNLISSKILADHMN
                     TYLEKLGPIPYTEQEYAYAQKFQQSLSDQDKNQLPTIARDYFGPKAPEAMKTPIFEPI
                     ACQNLYSDLKYSTDVGDVSWIVPTVHLNIPTFAAGTALHSWQAVAQGKSSIAHKGMLY
                     AAKIMALTALDFLQNPELANQARKELTETLNGETYPNPLPKDLKPEIW"
     gene            4037..4492
                     /locus_tag="ACZPD3_02545"
     CDS             4037..4492
                     /locus_tag="ACZPD3_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR1414919.1"
                     /translation="MEIERKFLISKENLPADLDAYPHHKLEQGYLSTAPVVRIRKEDD
                     NYYLTYKSKGLMTREEYNLPLTKESYEHMRPKADGILISKTRYLIPEKDRLTIELDVF
                     DAPYEGLYLAEVEFSSEEQALSYNPPVWFGKDVTNSGKYHNSRLSQGNL"
     gene            complement(4674..5468)
                     /gene="proC"
                     /locus_tag="ACZPD3_02550"
     CDS             complement(4674..5468)
                     /gene="proC"
                     /locus_tag="ACZPD3_02550"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR1414920.1"
                     /translation="MKIGFIGCGNMASAMISGMLKKGLYKKDEIIVSNLTEEGSKRSR
                     EKLGVVTTLDNHEVVKNTKLVFLAVKPQFYEEVLNEVKDELTPEHTVVGIAPGKTLAW
                     LEEKCGQPLKVVRMMPNTPAQVGEGMTGVCANEKVSAEELAQICEITDSFGRTEVVPE
                     RLMDAVSAVSGCSPAYVFMFIEAMADAAVAQGMPRKQAYQFAAQALLGSAKMVLETGM
                     HPGELKDMVCSPAGSTIEGVRILEQKGFRSAVFEALNGAAEKGKKM"
     gene            complement(5601..6047)
                     /gene="dtd"
                     /locus_tag="ACZPD3_02555"
     CDS             complement(5601..6047)
                     /gene="dtd"
                     /locus_tag="ACZPD3_02555"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR1414921.1"
                     /translation="MRFVIQRVMHSKVTINGEVRGQIGKGFMVLIGVGEEDTVEIADK
                     MIHKMVNMRIFEDENGKTNLGLKEVGGSLLLISQFTLYADCKRGNRPSFIRAGAPDMA
                     ENLYKYIIARCKEHIDIVEQGEFGADMKVELVNDGPFTVILDSNEL"
     gene            complement(6324..7682)
                     /locus_tag="ACZPD3_02560"
     CDS             complement(6324..7682)
                     /locus_tag="ACZPD3_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1414922.1"
                     /translation="MARSKEMDLTTGNPFRSLLKFAIPVILGNLFQLFYTLADSVIVG
                     KTLGADSLAAVGSTSIIIYFVFCFINGFTGGFGICLGQKCGAKDEKGMRKSVAVSTLL
                     SIAFTVVLTLTCCLLSHQILRWMQIPADISGEAYDYMFVVLLGTGATVFYNMISNMLR
                     ALGDSKTPLYFLVFSSVLNIFLDILFIVPFHMGVAGAAWATILSQFFSAVLSLVVGLK
                     NFPILHLHREDFTDLKDTAILHLKTGFPMGFQMSVMCIGQLAMQAVVNSLGTAAVAGY
                     TAASKADQLSVLVNNAMMTAISNYVAQNFGAGRWDRIRQGVRACLIQTEAFNLIMCIG
                     ILLLRHPIVRMFLSDPTKEIYHYSDMYLTIVAPFYFVLGLLAVYRTSIQSMQNGKAPF
                     AACMIELVMRIAATVGLSGIIGYTSVCIASPMAWIGACALLIPVYYKMMKGNCAALLR
                     ME"
BASE COUNT         2008 a         1785 c         1646 g         2568 t
ORIGIN      
        1 ttgtcagcag tcatgcctgt gaactggaag tactgatcca tggataatcc ctgagactgc
       61 attcttctgc tgaaatcatc aagcatctga cgacactgtg tcataagcat agcttccgga
      121 atatccatct gtgcattttc gattgcttta tctactgctg catcttcttt tgcacgtttt
      181 gcttcgtctg cttttttatc ttcaaggttt gtttttacat gctctttgta ctctgcaagt
      241 gtatcgaact cagaaacatc ttttgcaaaa tcatcatcca gttcaggaag ttcttttgct
      301 tcgatcttct taactgcgca tttgaagatt gcttctttac ctgcaagttc ttttgcctga
      361 tattcttcag ggaatgtaac ttttacgtct acatcatcgc caactttagc accaacaagc
      421 tgttcctcga atcccgggat aaatgtgttg gaaccaagtg tcagaggata ctctgtagcc
      481 tgtccgccat cgaaaggtac tccgtctaca gatccttcga agtcgattgt tacgatatca
      541 tttaactgag ctgcgcggtc ttctacgctg attgttctgg agtttttctc ctgctctttc
      601 ttgagttcag cttctacgtc ttcgtctgtt actgtgattt cagttttcgg tacttctaca
      661 cctttgtaat cacctaatgt aacttccggt ttaacagcaa cttctgctgt gaagatgaat
      721 gcttttccag gctcagcctg tgtaatgtcg attgtcggct gggaaacgat ctcaagttcg
      781 cattcagcta atgctttgct gtagtgttcc ggaatcagag cgttaacagc atcttcaagg
      841 aatactcctt tgccatacat ctgctcgatc atttttcttg gagcttttcc tttacggaat
      901 ccagggatgg aaattcttcc ttttgctttc tggtatgcat tctgcattgc tttttctaaa
      961 tcttcagcgg caacttcgat agtaagttta gccatgtttt tttccatttt ttccacctgt
     1021 aaactcatgt tacaatgttc ctccttaaat tcaggttata ttttgcgact ggtttttcag
     1081 ctgcgttata tcattcacag tttttaccta tttctatata ttcaagtctg agctccgctc
     1141 ccaccattgc agaagcggga gagttactgt tcactctgtg tcagtaacgc aagaagtctg
     1201 tttataactc aattatatat ttacagtcat tgtagcagta tagcacaaaa aatccaaaag
     1261 tgctagtgtt ttttcgcata aaaataaggg ttttgggatg ttttaacgtg tgtaaactac
     1321 agattctgcg atttcttttg ctttttcttc attttcgagc tgtgaaatca gggagagtgc
     1381 gaagtctaca gccgttccca ggccgcggct ggtggttatg tttccgtcta ctactacgct
     1441 gtcttcactt gttactgcgc catctttgga gagaacttcc atgaatgacg gataggaagt
     1501 tgctttgcgg cctttgagga atcccagtcc ggaaaatacg cttggagctg cgcagatggc
     1561 tgcgattttt ttgcctttat tgttgaagtc tgtcaggagg tcgatgagtg gtttgtaacc
     1621 ggcaaggtat ttggttcccg gcattcctcc aggaagaacg agcatgtcgg cgtcggcgaa
     1681 gtcggtttca gcgaatatgg cgtcggtgag catggtgatg ccgtgggatg tctggatccg
     1741 ggtggtttct ttgatagata cggttttgat gtcgattccg gcgcggcgaa gaaggtctac
     1801 tacggttaag ccttcaatgt cttcgaatcc atcggcgagg aagatgtata cttttttact
     1861 cattttttgt gcctcctggt tgttgtattt tttaagattt ttatcttgtt tttctgaaat
     1921 ggattatagc attttttatg gatagttaca atgtttgttt ggattgggta gggacactgg
     1981 gcagggacgc gcgaagccat ctgtctcctg gggttcagtt tcgtgagcct aagtaactct
     2041 aaggtttggt aaatctgcta aaaacatgaa aaagaatgca aaactcgctc cgttgcaaac
     2101 ttcgctcaga cacgttgcga ttcttttcca taacgcagat ttcccaaacc taagagttac
     2161 taagactcgt caaattcacc ccaggagaca gatggcttcg cgctgggaac tggcaaaatg
     2221 ttacttttac catatctctg gtttcaggtc tttcggtaat ggattgggat atgtttcgcc
     2281 gtttagagtt tctgtcagtt cttttctggc ttgatttgcc agttctggat tttgtaaaaa
     2341 atccagggca gtgagagcca tgattttggc ggcgtagagc attcctttgt gagctattga
     2401 ggatttgccc tgggctactg cctgccagga gtggagagcg gttcctgcgg cgaatgtggg
     2461 gatgttgagg tggacggttg gaacgatcca gcttacgtct cctacatcgg tggagtattt
     2521 gaggtcgctg tacagatttt gacaggcgat tggttcgaag attggggttt tcattgcttc
     2581 cggtgctttg ggaccaaagt agtcccgggc aattgtagga agttggtttt tgtcctgatc
     2641 cgagagggac tgctggaatt tttgtgcgta ggcatattcc tgctctgtat atggaatcgg
     2701 gccaagtttt tccaagtagg tgttcatatg gtctgcaagg attttactgg agattaaatt
     2761 ggaataggca gccacctgac ggatttctac ggtagtttcg gtcatgaggg cggcgccttt
     2821 ggctatatta catacacggt tgtagagttc ttttacctga gtgatcttcg gggcgcggat
     2881 ggcgtagagt acttgggctc tggactggac tacgtttggg gcatctccgc ctgtgtcggt
     2941 gatggcatag tggattctgg cttcgtctat catgtgttca cgcatgtagt ttacgcctac
     3001 gtccattaat tctactgcat ccagtgcgga acggcctaat tccgggcaca tggaggcgtg
     3061 tgccggggtt ccgtggaatg tgaagaagac tctgaagttt gctttggtgc ttccgtagca
     3121 tgcgcgtcgt ccctgttccg ggtgccagca gagggcgagg tcacagtcat cgaaatagcc
     3181 gtcgcgtacg agaaacgctt tgccgcctgc gttttcttcg gctgggcagc cgtagtagcg
     3241 gattgttcct ttcaggttgt gttctttcat gaagtctttt aatgcgatga cggaagcaag
     3301 ggtgcctgtt ccgataagct gatgaccgca gccatgacca caattatcag tatgtcctga
     3361 atctggattt tgtttctttt ctctttccgg cacgctttca tattttgaat ctgatgtttt
     3421 ttcttccggg attctctcgg tagtatctgc tttctgcgag agtcccggca gggcatcaaa
     3481 ttcgccaaga aatcctataa ccggttttcc ggaaccgtat tctgcaataa atgctgtttc
     3541 ctctcctgcc agatttgatt ttatggaaaa tccctctttc tttaatgtct gctgaagtaa
     3601 ttcagaagat ttatattctt gaaatctcgg ctccgcatac tcccagattt tatcgctgat
     3661 tttggcataa tattcttttt tatcttcaat tttttctgat atttctttta tgtattcatc
     3721 catatatttt tcctcttgtc ccatgctgtt tcctactttc ttcttcattc cccacaacat
     3781 ttttatctat tttacattaa gcagatatca tgttactgtt cacacaccac tatcccgaga
     3841 catacaagcc aaaattccat tgccgcaggc aatctggaat gaattttgtc tacgttactg
     3901 tgaacggagt gaacagtaac gatatcaccg tttgatttaa gtaaaatatg cgttttcatt
     3961 atttttcata tatgctatta tcattatagc acaaactaaa ggattgacac attttaactc
     4021 aatggaggaa atttaaatgg aaatcgaacg taaattttta atatccaaag aaaatctgcc
     4081 agcagatctt gatgcttatc cacatcataa actcgaacag ggctatttat ccactgcacc
     4141 ggtagtccgt atccgcaaag aggatgataa ctactatctt acctataaat ccaaaggtct
     4201 tatgacccgc gaggaatata atcttccact taccaaagaa tcctatgaac atatgcgtcc
     4261 aaaagctgac ggaatcctca tttccaaaac ccgctatctt atcccggaaa aagatagact
     4321 caccattgaa ctggatgtat ttgatgctcc ttatgaagga ctctatctcg cagaagtgga
     4381 attttcttct gaagaacagg cgctcagtta taatccaccc gtatggtttg gcaaagatgt
     4441 tacgaactcc ggtaaatatc ataacagtcg tctgagtcag ggaaatttat aataaaataa
     4501 aacagctccg aaatattgtt ctgttaatat gtatttgcag gaattgtagt aactcttagg
     4561 ttctggaaat tcgcgttata aaaaatccgc tcactgtctg agccgctttg cggcgagttt
     4621 gagcggattt tttatggttt tagcggattt tcagagcctt agagttactg agattacatc
     4681 tttttacctt tttcagcagc tccattcaga gcttcaaaga cagcacttct gaatcccttc
     4741 tgttccagga ttcttactcc ctcgatggta cttccggccg gtgaacatac catatccttc
     4801 agttctcccg gatgcatgcc tgtttccagt accatttttg cgcttccaag taaagcctgt
     4861 gcagcaaact gataagcctg ttttcttggc attccctggg ctaccgcagc atctgccatt
     4921 gcttctatga acataaaaac atatgcaggt gaacatccgc ttacagcact tactgcgtcc
     4981 atcagacgct ccggaactac ttctgtacgt ccaaagctgt ctgtgatctc acagatctga
     5041 gccagttctt ctgccgatac tttctcattg gcacatacac cagtcatacc ctccccaacc
     5101 tgagcaggag tattcggcat cattcttacc actttcagag gctgtccaca tttttcttct
     5161 aaccaggcaa gagttttccc cggcgcaatg cctacaactg tgtgttccgg tgtcagttca
     5221 tcttttactt cattcagcac ttcttcataa aactgcggtt tcacggccag gaaaaccagt
     5281 tttgtgtttt ttaccacttc atggttatcc agggttgtaa ccactcccag cttttctctg
     5341 cttcgtttgc ttccttcttc tgtaagattg gaaacaataa tctcatcttt tttataaagt
     5401 ccttttttca gcataccact gatcatggca gaagccatat ttccgcatcc gataaatccg
     5461 attttcataa atctgctcct ctcttgcaat tcctgttgtt ttctgataat ttataactgc
     5521 ttttttcata tctttttctt gttatagata atgataatca tccagcaatc tgccgtaaaa
     5581 tactgaacag ttacagataa ttataattca ttactgtcca atatgactgt aaagggaccg
     5641 tcattcacaa gctccacttt catatctgcc ccaaattctc cctgctcgac aatatcaatg
     5701 tgttctttgc atctggcaat tatgtatttg tacagatttt cagccatatc aggggcccct
     5761 gctcttataa aggaaggtct gtttcctctt ttacagtcag catataatgt gaactgggaa
     5821 ataagcagaa gacttccgcc cacctccttc aggccaaggt ttgtcttacc gttttcatct
     5881 tcgaaaatac gcatattaac cattttatga atcattttat ctgcgatttc aacggtatct
     5941 tcttctccta ctccgataag aaccataaag cccttgccta tctggcctcg tacttcacca
     6001 ttaattgtca cttttgaatg cattactctc tgaattacaa aacgcataat tcactctcct
     6061 taatcaacgg atttctgtct gttgatagat agcatagcac atttttatcc gtttttccat
     6121 tgtaaagtgt caccttgcag ctgttatctc aaaatcttgt gttactgttc actccgttca
     6181 cagtaactta gccaaaattc attccagatt gcctgcggca atggaatttt ggcttgtatg
     6241 tctcgagatt ttggcatatt catgccaaaa cacctcgcgg gatagtggta tgtgaacagt
     6301 aacaattttg ttacttgcac tcgtcactcc attctcagta atgctgcaca atttcctttc
     6361 atcatcttat agtaaaccgg gatcagaagt gcacaggctc cgatccacgc cattgggctg
     6421 gcaatacata cagatgtata tccgataatt ccggaaagac ctactgttgc agcaattcgc
     6481 ataaccagtt caatcataca ggcagcaaaa ggtgctttgc cattctgcat gctctggatg
     6541 gatgtacggt atactgccag cagtccaagc acaaagtaaa agggagctac gatcgtcaga
     6601 tacatatctg aatagtgata gatttctttt gtgggatctg agagaaacat tcttacgatc
     6661 ggatgtctca gcagcagaat tccaatacac atgatcaggt taaatgcttc tgtctgaatc
     6721 agacatgcgc gcacgccctg acggatacgg tcccatctgc ctgcaccaaa attctgagcc
     6781 acatagttgg aaattgcggt catcattgca ttgtttacca gtacagaaag ctggtctgct
     6841 ttcgatgctg cggtatatcc tgcaactgct gcagtaccca gtgagtttac cactgcctgc
     6901 atggcaagct gtccgataca catgacagac atctggaatc ccataggaaa tccggtcttt
     6961 aaatgcaaaa ttgcggtatc ctttaaatct gtaaaatctt ctctgtgcag atgcaggatt
     7021 gggaaatttt ttaatcccac taccagactc agcactgccg aaaaaaactg tgacagaata
     7081 gtcgcccatg cagcaccggc cacccccata tgaaacggta caataaacag aatatcaaga
     7141 aaaatattta atacagatga aaataccagg aaatacaatg gtgttttact gtctcccaat
     7201 gcgcggagca tatttgagat catgttgtag aaaacggtag cacctgtacc aagcaaaact
     7261 acaaacatat agtcgtatgc ttcaccggaa atatcagcag gaatctgcat ccagcgcaga
     7321 atctgatgag aaagcagaca gcaggtcagt gtcagtacta ctgtaaaggc aatactcaga
     7381 agtgtggaaa ccgcaacgct cttgcgcatt cctttttcat cctttgcccc gcatttctgt
     7441 cccagacaga taccaaaccc acctgtaaat ccattgataa aacagaatac aaaataaata
     7501 ataatactgg tggaacctac tgctgccagg gagtctgctc ccagcgtttt tcctacgatc
     7561 acagagtccg ccagtgtata aaaaagctga aacagatttc ccagaattac aggaatcgca
     7621 aatttcagca gtgaccggaa cggattacct gtagtcagat ccatttcttt tgatcttgcc
     7681 atgtttccta ctcctctcct tgcacgatgt gcataccaac gttataattg tggttataac
     7741 aagcattgta atgaatatat cctgtgataa attgtcagat cgtgttgatg atcatgcttc
     7801 tgtgagcaat tcatcacagc gtacatataa taaaatcgga gaactgtaat atggctatgc
     7861 gtaaaattct tgtagatgaa accaaccgcg aactttgtga acatggaact gtcggttttc
     7921 ccatgaccgt taatcatgat gacctgtggg catttgaggg aaaaagtgta cctatccact
     7981 ggcacaatga cctggagatc agtcttc
//
LOCUS       DBMDXG010000048         7838 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2447_length_7838_cov_2.743828, whole genome shotgun sequence.
ACCESSION   DBMDXG010000048 DBMDXG010000000
VERSION     DBMDXG010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2447_length_7838_cov_2.743828"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="ACZPD3_02565"
     CDS             <1..519
                     /locus_tag="ACZPD3_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1414923.1"
                     /translation="ISKVNMVFMAICIGISQGSQPIWGFNYGAKKYDRVRQAYRYSVT
                     ACTVIATIFFFCFQIFPHQIVGIFGTGSNLYFQFAERYLKIFMFMTFANGIQPVSSGF
                     FTSIGMAKLGIVMSLTRQVIFLLPLIIIFPLFMGIDGVMYAGPIADAAAFVLAIVFAR
                     RELGKMRSAEIV"
     gene            complement(605..1381)
                     /gene="thiM"
                     /locus_tag="ACZPD3_02570"
     CDS             complement(605..1381)
                     /gene="thiM"
                     /locus_tag="ACZPD3_02570"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR1414924.1"
                     /translation="MIVNQTNPRQLTRAAKPLIHCITNPISIHDCANIILAAGGRPIM
                     AEHPAEVAEITRSSHALALNLGNITDARMKSMPESLKTAASLHIPVMLDLVGTACSNL
                     RYEFAQKLMNIHMPELLKGNMSELLAMSGQTAHAIGIDAGVQDVLTDANRSHLKELFQ
                     EKASRWNTTLLITGKEDMIVSANKCEFITNGTPAMSQITGTGCMLGMICATYLAVTDP
                     FTAALSAAREFGTAGERAEKNSSGPGSFQTELFDQFYNLL"
     gene            complement(1311..1898)
                     /gene="thiW"
                     /locus_tag="ACZPD3_02575"
     CDS             complement(1311..1898)
                     /gene="thiW"
                     /locus_tag="ACZPD3_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HIR1414925.1"
                     /translation="MNTDRSKTLRMVMLAMMVAIGVVISPILRIEGMCPTAHLINIVC
                     SVLLGPWYSLLCATLIGIIRMMFMGIPPLALTGAVFGAFLSGVFYRASHGKIICAVIG
                     EIFGTGIIGSLVSYPVMAFLMGRSGLNAFFYTPMFLAATCMGGTIAYFFLKALSHAGM
                     LAKFQQSLGAKVYDRKSDKSQTIDPSSQTADSLHH"
     regulatory      complement(2013..2116)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2871..3746
                     /locus_tag="ACZPD3_02580"
     CDS             2871..3746
                     /locus_tag="ACZPD3_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1414926.1"
                     /translation="MKQENYIKGMIMIILSAFFFACMNVSVRLAGDLPSVEKSFFRNL
                     VAAVFAAIVLCKNRTVPKVDKKYWGPLILRCVCGTLGILCNFYAIDHLLVADASILNK
                     LSPFFAIIFSFLLLKEKIRPAQAACVALAFIGCLFVVKPGFQNAALVPALIGVCGGLG
                     AGIAYTMVRVLGTHGVKGPVIVFYFSFFSCLSVVPWMLFHFTPMSMKQLVTLLMAGLF
                     AAGGQFTITAAYTYAPAGKISIFDYSQIIFATMLGFVLFGEIPDKYSFTGYVLIILAS
                     LGTFLYNMRVAKAGK"
     gene            3916..4320
                     /locus_tag="ACZPD3_02585"
     CDS             3916..4320
                     /locus_tag="ACZPD3_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HIR1414927.1"
                     /translation="MSFIKNIPLRPHHGMCLAYFKGEGYSNGFSAHMQEMLDIFQKGA
                     KIQLHADTDEICSACPNNEKGCCSSFSLVEAYDNAVLELCGLENGQIMEFDDFTDVVQ
                     KKILTSGKRKEICGNCQWNSICESQKSRWEKC"
     gene            4425..5480
                     /locus_tag="ACZPD3_02590"
     CDS             4425..5480
                     /locus_tag="ACZPD3_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR1414928.1"
                     /translation="MENTESRLYFASDYMEGAHPAIMKKLMETNLEKTVGYGQDPYTE
                     DAKEKIRKACNAPEADVFLLVGGTQTNATVIDALLKSYQGVVAADTGHIATHESGAIE
                     FGGHKVLTVPQKDGKISAAQIEKLVKDFYDDTNYEHMVMPGMVYISQPTEYGTLYSRE
                     ELAALSKVCRENHLPLYVDGARLAYALASPENDVTLTDLAELSDIFYIGGTKCGALFG
                     EAVVIPQKGRIPHFFTIIKQHGALLAKGRIAGIQFGELFTDGLYLRIGKPAMEAAEQI
                     KDALKKYGYQLSLDTPTNQIFCIVSNEVMKKIAQDVEFGFWEKYDETHSVIRFATSWA
                     TTMEDTQKLIQILEKNK"
     gene            complement(5490..6653)
                     /locus_tag="ACZPD3_02595"
     CDS             complement(5490..6653)
                     /locus_tag="ACZPD3_02595"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1414929.1"
                     /translation="MKFYSEKLSPRRLSLQWRLTLLISGLVITACALMYFFISRSAVT
                     GLEGISDYVVLVTPDNSNPISINVDPKILIPDLENQIQNTKNNFLFQSIIATGIIILL
                     SSICTWFVTRRALAPLRRFSDRISQVQAQNLSEPLEVPLSEDEISRLTRSFNDMLARL
                     DNAFSAQKQFVASAAHELRTPLAVMQTNLEVFYKKPEHAPQEYDRLFTMLQEQTGRLS
                     HLAEILLDMTGLQTVERSDTISLAALTEEVFCDLDPVADKHQIRLIQTEGDCTVTGSY
                     ILLYRAVYNLVENAIKYNRPSGSVTVNIHSAESAVLEVTDTGIGISPENQEKIFDPFY
                     RVDKSRSRAMGGAGLGLALVSEIARQHNGQVKVTQSSEKGSTIALILPKSTDY"
     gene            complement(6735..7403)
                     /locus_tag="ACZPD3_02600"
     CDS             complement(6735..7403)
                     /locus_tag="ACZPD3_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1414930.1"
                     /translation="MHILIIEDEEQLCRSMAEGLRMDGYETDTCFDGEEGLELCMTEN
                     YDLILLDLNLPGIDGLEILRQFRTFNTNTPVLILSARVQIQDKVEGLDLGANDYLTKP
                     FHFEELEARIRSLTRRKFIQEDVCLRCSRITFDTRTREASVDGTPLALTRKESALLEY
                     FLLHRNRIISPEEMIEHLWDGSVNSFSNSIRVHISSLRKKLRTALGYDPIQNKIGQGY
                     ILEE"
BASE COUNT         2199 a         1524 c         1905 g         2210 t
ORIGIN      
        1 atctccaaag tcaatatggt gtttatggca atctgtattg gtatttctca gggcagtcag
       61 ccaatctggg gatttaacta cggagcaaag aaatatgaca gagtaagaca ggcataccgt
      121 tattctgtaa ctgcctgtac agtaattgcg acgatattct ttttctgttt ccagatattt
      181 ccacaccaga ttgtgggtat cttcggaaca ggaagtaatc tgtatttcca gtttgcagaa
      241 cgctatctga aaatatttat gtttatgact tttgcaaatg ggattcagcc ggtgtcttcc
      301 ggtttcttta cctctatcgg tatggcgaag ctgggtattg taatgtctct gacacgacag
      361 gtgatttttc ttctgccatt gattattatc ttcccattgt ttatgggaat tgatggagtg
      421 atgtatgcag gaccgattgc agatgcggcg gcatttgtgt tggcaattgt gtttgcgaga
      481 cgagaactgg gaaagatgcg cagtgcggaa atagtataaa aagttcccgc agatgatatg
      541 gaagaaattt atccatattg tctgacggga acttttttgt aaaaaattat tgataaactg
      601 tagattataa aagattgtaa aattgatcga acagctctgt ctggaagctt ccggggccgg
      661 agctgttttt ctctgctctt tctcctgctg tgccaaattc tctggctgct gagagggctg
      721 ctgtaaatgg gtctgtaact gccagatagg tggcacagat cattccaagc atacatccgg
      781 ttcctgtgat ctgtgacatg gcaggagtac cgtttgtaat aaattcacac ttattggcag
      841 agacgatcat atcttccttg cctgtgataa gaagtgtggt attccaccgg gaagcctttt
      901 cctggaaaag ttctttcaga tgggaacgat tggcatctgt aagtacatcc tgtacgcctg
      961 cgtcaattcc gattgcatgt gcggtctgtc cggacatggc aagaagttca gacatatttc
     1021 cttttaggag ttcaggcatg tggatgttca taagcttctg tgcgaattca tagcgcagat
     1081 tgctgcaggc agttcctacc agatcaagca taacagggat atggagactg gcagcagttt
     1141 tcagagattc cggcatggat ttcatacgtg catcagtgat atttccaaga ttcagtgcca
     1201 gtgcatgaga gcttctggtg atctcagcta cttctgccgg atgttctgcc atgatcgggc
     1261 ggcctccggc cgccagaata atattcgcac agtcatgaat agaaatcgga ttagtgatgc
     1321 agtgaatcag cggtttggct gctcgggtca attgtctggg atttgtctga tttacgatca
     1381 tatacttttg ctccaaggct ttgctgaaat ttggcaagca ttcctgcgtg acttaaggcc
     1441 ttcaggaaga agtaggcaat ggtgccgccc atgcaggttg ctgccagaaa cattggtgtg
     1501 tagaaaaatg cgttcagtcc gcttctgccc atgagaaatg ccatcacagg ataggaaacc
     1561 agagagccga taataccggt tccgaagatc tcaccgatca ctgcgcagat aatctttcca
     1621 tgagaagcgc ggtagaaaac gcctgataaa aatgcgccga atacggcacc tgtcagtgca
     1681 agtggcggga tacccataaa catcatgcgg atgattccga tgagagtggc acaaagcaat
     1741 gaataccacg gtcccagaag tacggaacag acgatgttta tcaggtgtgc agtagggcac
     1801 ataccctcaa ttctcagaat gggtgagatg acaacgccga tggcaaccat catggcgagc
     1861 atgaccattc tgagtgtttt tgatctgtct gtgttcataa taatgaacct ccttgaaatg
     1921 aagatttggt ctgccgaagc agacattatc agttgtgcag gaagcagata aaatcctgca
     1981 aaaaaatctg ccggaataac acactggcag attttaatat acaaatacag tccctacgct
     2041 ggcattatcc atatcaggtg cggtcgaaga tcaatcttcc tctcagcctg tctcacaggc
     2101 tcccgttgtt attatccatt atgcagattt tcttgtgtta gagcgtattt gaaaaatgct
     2161 ttacgcaaga tgtgcgttac aatttttcaa acacgcttta gagtaacaca agcttttgga
     2221 aaagtcaatg ggatgtaaaa gcagggtaaa ttttctacag taaatttgtt actgttcaca
     2281 cgccactgtc ccgcgaggtg ttttggcatg aatatgccaa aatcccgaga catacaagcc
     2341 aaaattccat tgccgcaggc aatctggaat gaattttggc tacgttactg tgaacggagt
     2401 gaacagtaac gtaaatttac actacctaat attattttta agttgacttt ttattttttt
     2461 cagcatatac taacagtagc taaggaatgt gacgagattc catgaagcga cctaaagaaa
     2521 caaaagccct cagagttgca gctctgaggg ctttcttgct cgttacggtg agctgttccg
     2581 tttaggctgt tgctgtctaa cgtctgccgt ccagccattt gcatatgtag tagctaactg
     2641 cacctgctaa aacagagaca agaaatgtga cgagaatttc ttccatgaaa ctcacctcct
     2701 ttctgctgga ggttcgacag ctcttatagt ataacatggt ttaggacgaa ttggaataaa
     2761 aaatctattt gtactaaaac agttgtccgt gctatgatta acaggtagat gacagacagc
     2821 atatgataaa aagaagctgt ctgcatggac agaaaggcag gttaaatctg atgaaacagg
     2881 aaaattacat aaagggtatg ataatgatca tcctgtcggc gtttttcttt gcttgtatga
     2941 atgtgagtgt gcgtctggca ggtgatctgc cgtctgtgga gaagagcttc ttccgaaatc
     3001 tggtggcggc ggtgtttgca gcgatagttc tctgtaagaa tcgtacagtg ccgaaggtgg
     3061 ataagaaata ctggggacca ctgatcctgc gttgtgtgtg cgggacactg gggattctgt
     3121 gtaactttta cgccattgac catctgttgg tggcagatgc atcgatcctg aataagttat
     3181 ctccgttttt tgcgattatt ttttcattcc ttttgttaaa ggaaaagatc agaccggcgc
     3241 aggctgcgtg tgttgcgctg gcgtttatcg gatgtttatt tgtagtgaaa ccgggatttc
     3301 agaatgcggc acttgttccg gcactgatag gagtctgcgg tggactgggg gcaggaattg
     3361 cctatacgat ggtgcgtgta ctgggaactc acggagtgaa gggaccggtt atcgtgtttt
     3421 atttttcatt tttctcctgc ttgtcggttg taccctggat gttgttccat ttcacaccca
     3481 tgagtatgaa acagcttgtg acattgctga tggcaggatt attcgcagcg ggcggacagt
     3541 ttacgattac ggctgcatac acctatgcac ctgcagggaa gatttccatc tttgattatt
     3601 cgcagattat ttttgccacg atgctgggat ttgtcctgtt tggagagatt ccggataagt
     3661 acagctttac aggatatgtg ctgattattc tggcatcatt gggtaccttt ctttataata
     3721 tgagggtagc taaggctggt aaatgaagaa aaagtaaaaa aggattttct gattttatcc
     3781 agtaagtctg atgaaatccg atcagtatat agaattattg gattgtaaac tataaaataa
     3841 aaaaagttgt caatagaaac gctttgtgtt aaagtatcca atatgttaaa aaattaagga
     3901 gaaaggtgca caaagatgtc ttttataaaa aatattccac tacgccctca ccatggaatg
     3961 tgccttgcat atttcaaggg tgagggatat agtaacgggt tttccgcaca tatgcaggaa
     4021 atgctggata ttttccagaa gggagcaaag atacagcttc atgcagatac agacgagatc
     4081 tgttctgcct gtccgaataa tgaaaaagga tgctgttctt ccttttcact tgtagaagca
     4141 tatgacaatg cagtgctgga actatgcggc ctggagaatg gacagatcat ggaatttgat
     4201 gattttacag atgttgttca aaagaaaatc ctgacatcag gaaagaggaa agaaatctgt
     4261 gggaattgtc agtggaactc catctgtgaa agtcagaaga gcagatggga gaaatgctaa
     4321 gaaaaataga gtaacagaat gaatagcgtt ccggtacaga gcagaagcta tgaataaaaa
     4381 agtcaaatgc gcaaatagtg cagtgaaagg acataacgat aaaaatggaa aatacagaat
     4441 cacgtttata tttcgccagt gactacatgg agggtgcaca tcctgcgatc atgaaaaagc
     4501 tgatggagac aaatctggag aaaacagtgg gctatggtca ggatccatac acagaagatg
     4561 cgaaagaaaa gatcagaaaa gcctgcaatg caccggaggc agatgtattt ctgctggttg
     4621 gtggaacaca gaccaatgcc acagtgatcg atgcactttt gaaatcctat cagggagttg
     4681 tggctgcgga tacagggcat attgcaactc atgaatcagg tgccattgaa tttggcggac
     4741 ataaggttct gacagtaccg cagaaagatg ggaaaatctc tgcagcgcag attgaaaagc
     4801 tggtgaagga tttctatgat gataccaatt atgagcatat ggtaatgccc gggatggtat
     4861 atatttccca gccaacagaa tatggtaccc tgtattccag agaagaatta gcagctctca
     4921 gcaaagtctg ccgggaaaat catcttcccc tttatgtgga tggcgcccgc cttgcctatg
     4981 cactggcaag cccggagaat gatgtgacgc tcactgatct ggcagaactt agcgatatat
     5041 tttacattgg aggaaccaaa tgcggagcat tgtttggaga agcagtggtt ataccgcaga
     5101 aaggacggat tcctcatttc tttacgatca tcaagcagca tggagcattg cttgccaagg
     5161 gacggatcgc aggaatccag tttggtgagc tgtttacgga cgggctgtac ctgcggatcg
     5221 gcaaacctgc aatggaagct gcagagcaga tcaaggatgc actgaagaag tatggatatc
     5281 agctttctct ggatactccc accaatcaga tattctgtat tgtgtccaat gaggtaatga
     5341 aaaaaatagc ccaggatgtg gagttcggat tctgggagaa gtatgatgag acacactctg
     5401 tgatccgctt tgccacaagc tgggctacta ctatggaaga tacacagaaa ctgattcaga
     5461 ttctggaaaa aaataaataa gagaaaaaat caatagtcag ttgatttagg cagtatcagg
     5521 gcaatggtac tgcctttttc gctgctctgg gttactttta cctggccgtt gtgctgtctg
     5581 gcaatctcgc ttacaagagc aagccccaga cctgcaccgc ccatggcgcg gctgcgggat
     5641 ttgtccacac ggtagaaggg atcaaagatt ttttcctgat tttcagggga gataccgatg
     5701 cctgtgtcag ttacctccag gactgcagat tctgcagaat gaatgtttac agtaacggaa
     5761 ccggaaggcc ggttatattt gatcgcattt tcaaccagat tatagactgc gcggtacaga
     5821 aggatatagc tgcctgtaac agtacagtca ccttctgtct gaataagccg gatctgatgc
     5881 ttgtctgcaa ccggatccag atcgcagaaa acttcttctg tgagtgctgc aagggaaatt
     5941 gtgtcagaac gctctacagt ctgaagacct gtcatgtcca gcaggatttc tgcaagatgg
     6001 gaaagccgtc cggtctgttc ctgaagcatg gtgaaaagcc tgtcgtattc ctggggtgcg
     6061 tgttccggtt ttttgtaaaa aacttccaga tttgtctgca ttacagctaa gggtgtgcga
     6121 agctcatgtg ctgcacttgc cacaaactgt ttctgtgcag agaaggcatt gtccagacgg
     6181 gcaagcatgt cattaaagga tctggtcaga cgtgagatct catcctctga gagtgggact
     6241 tccagcggct cagacagatt ctgtgcctgt acctggctga tcctgtcgct gaatctgcgc
     6301 agaggagcca gggctcttct ggtgacaaac caggtacaga tactgctgag aagaataatg
     6361 atacctgtgg cgattatgct ctggaacaga aagttgtttt tggtattctg gatctggttt
     6421 tccagatcag ggatgaggat tttaggatct acattgatgg agatcgggtt tgaattgtca
     6481 ggggtgacca gaactacata gtcggaaatc ccttccagac cggtcacagc agaacggctg
     6541 atgaagaaat acatcagtgc acaggctgtg ataaccagcc cggagatcag caatgtgagt
     6601 cgccactgga gggagagcct gcggggggaa agtttttctg aataaaattt catagataaa
     6661 tcctttcgtt tcattataat gacatagtag tatttgaaat ctgaaaatat ttattgtaca
     6721 gatatgaatg atttttattc ttccagaata tatccctgtc caatcttatt ttgtatagga
     6781 tcatatccaa gggcagttcg cagttttttt cttaaagaag aaatatgcac ccggatggaa
     6841 ttactgaagc tgttcacgct gccgtcccag agatgctcga tcatttcctc agggctgatg
     6901 atccggttgc ggtgaagcag aaaatattcc agaagtgcgc tttctttcct ggtgagagcc
     6961 agaggcgtgc catctacact ggcttctctt gtacgtgtgt caaaggtaat gcggctgcat
     7021 ctcaggcaga catcctcctg gataaatttg cgtcgggtaa ggctgcggat gcgggcttcc
     7081 agctcttcga aatgaaatgg cttggtgagg taatcgttgg ctcccagatc cagcccctct
     7141 accttatcct ggatctgtac tcttgcggag agtatgagaa caggagtatt ggtattgaag
     7201 gtacggaact gacgcaggat ttccagaccg tcaattcccg gcaggttcag atccagaagg
     7261 atcaggtcgt aattctctgt catacataat tccagtccct cttctccgtc aaagcaggta
     7321 tctgtttcgt atccgtccat gcggagcccc tctgccatgg aacggcagag ctgttcttca
     7381 tcttctataa ttaaaatgtg catgatagtc tcctttttga atttctagat ttatagtttt
     7441 atcatagcac acgaagtata aaattcgtgt aaaaaaagaa aatgtttcat attaaaatat
     7501 ccgattacaa agttctttta acaaccgtag caagaattcc ctcacctcta tagggacgcc
     7561 gctgaaaaac tgttttttca tactatatac agtgtctcaa agttgattga tgtactttat
     7621 tttgtgttaa tttatacgca aaaaggactt tttcagtggc cttctatagg tgatgggatg
     7681 aattgcacta tgaattgcac attcgagcat gtctgctcaa atttataaaa aatcacaaaa
     7741 cacttgttct gacacaataa atatattata atagaaaaaa agaacaatat gctcttgaaa
     7801 tttggacggt cgaaataagc aataagtgag tgtacata
//
LOCUS       DBMDXG010000049         7772 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2470_length_7772_cov_1.548820, whole genome shotgun sequence.
ACCESSION   DBMDXG010000049 DBMDXG010000000
VERSION     DBMDXG010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_7772_cov_1.548820"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="ACZPD3_02605"
     CDS             <1..749
                     /locus_tag="ACZPD3_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1414931.1"
                     /translation="WYEGKECDTKSREDILKSVRTLARLHILMKMEPVEEYRERSLRE
                     EYLRHNQELRKIRKFIRNKGASNVFEKNYLASVEQFLERAQYAVKLLDETDYDDLRER
                     AWREGQVCHGEYNQHNVLMLKGDHLGTAVTNFGHWSFDIQVADLYRFMRKILEKYNWN
                     LELAGEMLREYHKIRPISAQEWKNLRVRFTYPEKYWKLANYYYSHKKVWISEKNVEKL
                     QNLIRQREIWENFAEECFRDYPRYCSLRSQ"
     gene            892..1170
                     /locus_tag="ACZPD3_02610"
     CDS             892..1170
                     /locus_tag="ACZPD3_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR1414932.1"
                     /translation="MNKTELVAAMAKETNLSKKDVEDVLKSFVDVVSKELKNGGKIQL
                     VGFGTFEVSERAAREGRNPQTGETMKIEASKSPKFKAGKALKDMVNGK"
     gene            1269..1508
                     /locus_tag="ACZPD3_02615"
     CDS             1269..1508
                     /locus_tag="ACZPD3_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1414933.1"
                     /translation="MRLDKYLKVSRLIKRRTVANEACDAGRVLVNDKPAKASVQVKTG
                     DTIEIQFGSKNVKVEVLDVKETVKKEDVESMYKYL"
     gene            1665..1946
                     /gene="yabP"
                     /locus_tag="ACZPD3_02620"
     CDS             1665..1946
                     /gene="yabP"
                     /locus_tag="ACZPD3_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HIR1414934.1"
                     /translation="MEEKKILSTHSLMLENRQNGRITGVKDIKSFDEKEILLFTQAGK
                     LVIKGEQLHVKQLDLEKGEVDLEGKVDSLTYLSKNTDNRDESLFRRMFR"
     gene            1950..2408
                     /gene="yabQ"
                     /locus_tag="ACZPD3_02625"
     CDS             1950..2408
                     /gene="yabQ"
                     /locus_tag="ACZPD3_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HIR1414935.1"
                     /translation="MSAFIHHELLLLLRTIAVGAVLLLCYDLLAAFRNVFAHSDKAVG
                     TEDILYWLCCAFFVFSEIYRSNEGILRFFLVLGFLSGAYICHRILGDIFVKCCTKFME
                     IPVIIIKFLIKWLLFPVKRCKLLWYKAYKCAKKGRLANWVILRKGIKKRK"
     gene            2369..2683
                     /locus_tag="ACZPD3_02630"
     CDS             2369..2683
                     /locus_tag="ACZPD3_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR1414936.1"
                     /translation="MGNSKKRNKKKKVGYNSLGMIAIALVVLVLLGGLMLESNDLKER
                     LTGYDAKAATLQQQIEDEQTRTEEIDKLKKYMETDEYAEEVAREKLGLVKDNETVFKK
                     QQ"
     gene            2870..4363
                     /locus_tag="ACZPD3_02635"
     CDS             2870..4363
                     /locus_tag="ACZPD3_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR1414937.1"
                     /translation="MQEWMIAMLVISIMLIIRDMAKTILAENLPDTEELPSLQEGHPQ
                     KERVEKYAASFQKLADTFYGMPYRKDYLSSRQVEQIIEDTNAKVCSRCYQREICWGEH
                     SQELVKGVEALVRSMESGNEENVRGIRADWTGICPRSVQYYETVAENFQKERQNLMWD
                     NRMIENRLAVAQQLMEVSHIMENVASDLYDLERVTPQFEEELRKGLRKSHVILRRAWM
                     MNKVKGRKQIFLTMRARSGQCISMTEIAQLLSKYCEISMVPVNGSRCIVNGEYHTVHF
                     AEDVSYQVLYGTARLTREEEKVSGDNYICRQEDGGRFVMCLSDGMGSGMEACRESETV
                     VELLEQFMESGFSQETAAKMVNSALVLKGEEGMFSTVDICAVDLYTGICNFLKAGAAS
                     TFIKRDHWVESITSESLAAGLVQQIDFETATRKLYHGDYLIMMTDGVLDALPDQKEEE
                     TMKEIIMDVHEESPKDFGRGILERVLGYSDYHARDDMTVLVAGVWKK"
     gene            4404..5861
                     /gene="tilS"
                     /locus_tag="ACZPD3_02640"
     CDS             4404..5861
                     /gene="tilS"
                     /locus_tag="ACZPD3_02640"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR1414938.1"
                     /translation="MEREILAYIKQNRMIGKNDVVLAGVSGGSDSMAMLRILKELQGK
                     LDFTLRVVHIHHGIRGKEADRDQSFVENICRKWQIPCTVYCYDVPGLSREWKLGEEET
                     GRIVRKEAFQREAAVCGRKDSEIKIALAHNQEDLAETMLHNLCRGTGLRGLCTMRPVD
                     GEIIRPILCLSRDKIAEYLKEKKISHIQDSTNLSDEYTRNRIRHHILPILEQQVNGKA
                     AAHMAETAARISQAEEYLTQQSCLVLGEFQKGKEYYFTEKFFMEPQIIQVYALQQAME
                     QLAGRRKDLAAVHYEKVLELYEMQTGRRISLPYHMEARRDYEGVRLCRYGEEKSAGMI
                     GEKHKGSDIQNGKTVCGKNISDREWKIRIPGTVTSPLGIFSAEIFLYEGQKIEEKKYT
                     KWMDYDKIKKNPYIRTRRTGDYMVINAQGNTKKLNRCMIDEKIPSEYRDSIPLIACGK
                     EILWMVGSRMNERYKINPKTRKVLVLNYQGGNENE"
     gene            5854..6384
                     /gene="hpt"
                     /locus_tag="ACZPD3_02645"
     CDS             5854..6384
                     /gene="hpt"
                     /locus_tag="ACZPD3_02645"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR1414939.1"
                     /translation="MSEKIKVLLSEEEVDSRIKQIAAKVSKDYAGKEIHLICVLKGGV
                     FFTCELAKRITVPVSLDFMSVSSYGDDTKSSGVVKIVKDLDQPLEGKDVLIVEDIIDS
                     GRTLSYLIEILKQRNPNSIRLCTLLDKPERRVRDVRVDYCCFNIPDEFVVGYGLDYAQ
                     KYRNLPFIGVVELGED"
     gene            6401..>7772
                     /gene="ftsH"
                     /locus_tag="ACZPD3_02650"
     CDS             6401..>7772
                     /gene="ftsH"
                     /locus_tag="ACZPD3_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1414940.1"
                     /translation="MNKQPSRIGLYLVLIIALVAGYFYLNNQVVSQSNYTQKQLEQAL
                     EDNKVVDATIQQNREVPTGSVIVDTTDSGQKKVYVTDVNQAIELLNKYDIDITTQDVP
                     GDNVFLTTLLPVLLTGALVIFLIMMMNRQMAGGGGGGNAKMMNFGKSRARMSSPDDNK
                     KVTFDKVAGLQEEKEDLAEVVDFLKSPQKYTKVGARIPKGVLLVGPPGTGKTLLAKAV
                     AGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEEGKRHAPCIIFIDEIDAVARQRGTG
                     MGGGHDEREQTLNQLLVEMDGFGVNEGIIVMAATNRVDILDPAILRPGRFDRKVAVGR
                     PDVKGREEILRVHAKDKPLGEDVDLTQIARTTAGFTGADLENLLNEAAIEAARRGRGF
                     ILQSDIKGAFIKVGIGAEKKSKVISEKEKKITAYHESGHAILFHVLPDMDPVYTISII
                     PTGMGAA"
BASE COUNT         2476 a         1284 c         2082 g         1930 t
ORIGIN      
        1 actggtatga aggaaaagag tgtgacacaa aatcccggga agatatttta aaaagtgtcc
       61 ggacactggc aaggttacat attttaatga aaatggaacc tgtggaggaa tacagggaaa
      121 gatctctcag ggaggaatat ctccggcaca atcaggagct tcgtaagata cgcaaattta
      181 tacggaacaa aggagcatcc aatgtatttg agaaaaatta tctggcaagt gtggagcagt
      241 ttcttgaaag ggcacagtat gcagtaaagc ttctggacga gacggattat gatgatctca
      301 gggaaagggc atggcgggaa ggacaggtat gtcatggaga atataatcag cataatgttc
      361 tgatgctgaa aggtgatcat ttggggacgg cagtcacaaa ttttggtcac tggagttttg
      421 atattcaggt agcagatttg tatcgtttta tgagaaaaat actggagaaa tataactgga
      481 atctggaatt ggcaggggaa atgctcaggg aatatcataa aatccgtcca atctcggcac
      541 aagagtggaa aaatctgcgt gtacgtttca cttatcctga aaaatactgg aaactggcaa
      601 attattatta ttcacacaaa aaggtgtgga tttcagagaa aaatgtggaa aagcttcaga
      661 atcttatcag acagagagaa atctgggaga attttgcaga ggaatgtttc agggattatc
      721 ctcgttactg ttcactccgt tcacagtaat ttatcctcag taacacagag ctgaacagtt
      781 acgaaaaacc ttgacaatat ggggataaag gtatatatat agaggataga gctctgtcgc
      841 ttttggcaga tgccaccaaa caagaaagag atcattagag gaggaaacac catgaataaa
      901 acagaattag tagcagctat ggcgaaagag acaaacttat ccaagaagga cgtagaagac
      961 gttctgaaat ctttcgttga tgtcgtttct aaagaactga aaaatggcgg aaaaatccag
     1021 ttagtaggat ttggtacatt tgaagtaagt gaaagagctg ctcgcgaggg aagaaatcct
     1081 cagactggtg aaactatgaa aatcgaagct tctaaatcac cgaaattcaa agctggaaaa
     1141 gcattaaaag atatggttaa cggtaaataa taaaggcgat aaccggggga acgaaagttc
     1201 cccttttttg atactccggc ggagtaattc gtgagtatcc atttacatga catttacagg
     1261 agcaaaatat gagattagat aaatacttaa aggtgtcacg tctgatcaaa cgccgtacag
     1321 tggcaaacga agcatgtgat gcaggaagag ttcttgtcaa cgataagcct gccaaggcat
     1381 ctgtacaggt aaagacggga gatactattg aaatccagtt cggaagtaag aatgtcaaag
     1441 tagaagtact ggatgtaaaa gaaactgtca aaaaagaaga tgtagagagt atgtacaaat
     1501 atctttgatt ccggatggta atttgcagct tttgccattt gtgcagtggg aacatataaa
     1561 tttttaacat tttattgtct atttccattt tccggcacat aaactactga taagtaacag
     1621 attggcaggt catagtttcc ggaggctgtg accgggaggc gtctgtggaa gagaagaaga
     1681 tactttcaac tcacagcctg atgctggaaa accgtcagaa cgggagaatt accggggtaa
     1741 aggatattaa atcctttgat gaaaaagaaa tcctgctttt tacacaggca gggaaacttg
     1801 taataaaggg agaacagctt catgtgaagc agcttgatct ggaaaaggga gaagtagatc
     1861 tggaaggaaa agtggacagt cttacctatc tgtcaaagaa tacagataac agggatgaat
     1921 ccttatttag aaggatgttc cggtgagaga tgagtgcatt tattcatcat gaattacttc
     1981 tgctgctccg gacaatagct gtgggtgctg ttctcctttt gtgttatgat ctgctggctg
     2041 cattcaggaa tgtttttgca cattcggata aagctgtcgg gacggaagac attctatact
     2101 ggctttgttg tgcctttttt gttttttcgg agatttacag aagcaatgaa ggaatcctgc
     2161 gttttttttt ggttttaggc tttttatccg gagcgtatat ctgccacagg attctaggag
     2221 atatttttgt aaaatgctgt acgaaattta tggagattcc tgtaataatt attaaatttt
     2281 tgataaaatg gttgctattt ccggtcaaaa gatgtaaact tctgtggtat aaagcgtata
     2341 aatgtgctaa aaaaggaagg ctggcaaatt gggtaattct aagaaaagga ataaaaaaaa
     2401 gaaagtaggg tataactctc ttggaatgat tgcgattgcg ctggttgttt tagtccttct
     2461 gggaggactg atgctagaaa gtaatgacct taaggaacgc ctcacaggat atgatgctaa
     2521 ggccgccact cttcagcaac agattgaaga tgagcagaca cgtacagaag aaattgataa
     2581 attaaagaaa tatatggaga cagacgaata tgcagaagag gttgccagag agaaactggg
     2641 acttgtgaag gacaatgaga ctgtttttaa gaaacagcag taacaaatgt cgaaaacttt
     2701 tgcgaaggat tcgtctgcgt ttgacagata tttcagaaac ccttccatat aatatctgta
     2761 actgtttagt actttgtgaa agagaggact gttgaacagt tattgaaatt ctctgataaa
     2821 aatgcagtag tctgctatat acagggaatg aataagggga ggggagaata tgcaggaatg
     2881 gatgattgcc atgctcgtta tcagcattat gctgatcata cgggacatgg caaaaactat
     2941 tttagcagaa aatctgccgg atacagaaga actgccgtct ctgcaggagg gccatcctca
     3001 gaaagagagg gtggagaagt atgcggcatc ttttcagaaa ctggcagata ctttctacgg
     3061 gatgccttat cggaaagatt atctgagcag caggcaggtt gagcagatca ttgaggatac
     3121 aaatgcaaag gtatgcagca gatgctatca gagggaaatc tgctggggag aacacagtca
     3181 ggaactggtg aaaggagtgg aggcactggt acgttctatg gagagtggaa acgaggaaaa
     3241 tgtcagaggc atccgcgctg actggacagg catatgtccc cgttccgtac agtattatga
     3301 gacggttgca gagaattttc agaaagaaag gcagaacctg atgtgggata accgtatgat
     3361 cgagaatcgg ctggcagtag cacagcagct tatggaagtc tcacatatca tggagaacgt
     3421 agccagtgat ctgtatgacc tggagcgagt gacgccgcag tttgaagaag aacttcgcaa
     3481 gggactgaga aagagccatg tgatccttcg cagggcatgg atgatgaata aggtgaaagg
     3541 acgtaaacag atttttctca ccatgcgtgc cagaagcggt cagtgtattt ccatgacaga
     3601 gatcgcacag cttctttcaa aatactgtga aatttccatg gtacctgtaa atgggagcag
     3661 atgtattgta aacggagaat atcacactgt acattttgct gaggatgtaa gctatcaggt
     3721 tctctatgga acggccaggc ttaccagaga agaagagaag gtttccggag ataattatat
     3781 atgcagacag gaggatggcg gcaggtttgt tatgtgcctt tcggatggta tgggctcagg
     3841 gatggaagca tgcagggaga gtgagacagt tgtggaactt ctggaacagt ttatggagtc
     3901 aggattttcc caggaaacgg cagcaaagat ggtaaattct gctctggtac tcaaaggaga
     3961 ggagggtatg ttttctacag tggatatctg cgcagtggac ctgtatacag gaatctgcaa
     4021 ttttctgaag gcgggggcag cgtctacatt tatcaaaaga gatcactggg tggaatctat
     4081 tacttctgaa agtctggccg cagggcttgt acagcagatt gattttgaga cagctaccag
     4141 aaagctgtac catggggatt acctgatcat gatgacagat ggagtcctgg atgcattgcc
     4201 tgaccagaaa gaggaggaaa ccatgaaaga aatcattatg gatgttcacg aggaatcacc
     4261 gaaagatttc ggcaggggaa tactggaacg ggtgctggga tacagcgatt atcatgcacg
     4321 ggatgatatg acagtgctgg tggcaggcgt gtggaaaaaa taatgctgcg gatactaaga
     4381 tagaaaagga aatcaggata aaaatggaaa gagaaattct tgcttatata aagcaaaaca
     4441 ggatgatcgg aaaaaatgat gtggtcctgg caggagtgtc cggcggaagt gactctatgg
     4501 caatgctccg cattttaaag gagcttcagg gaaaactgga ttttacattg agagtggtcc
     4561 atatacatca tggaatacgt ggaaaggaag cggacaggga ccaaagcttt gtggaaaaca
     4621 tatgccggaa gtggcagatt ccatgtactg tttattgtta tgatgttccg ggattgagcc
     4681 gggagtggaa gctgggcgag gaagagacgg gaagaattgt aagaaaagaa gcttttcaga
     4741 gggaagctgc cgtctgtggg agaaaggaca gtgagataaa aattgcatta gcgcataatc
     4801 aggaggatct tgcggaaact atgcttcata atctgtgcag aggaacaggt ttgcggggac
     4861 tatgcaccat gcgccctgta gacggagaga ttatccgccc gattctgtgc ttaagccggg
     4921 acaaaattgc agagtatctg aaagaaaaga agatttctca tattcaggac agcaccaatc
     4981 tgtcagatga atataccaga aaccgaatca gacatcatat tcttcctata ttggaacagc
     5041 aggtcaatgg aaaggcagcg gcacatatgg cagagactgc ggcaaggatt tcccaggcag
     5101 aggaatatct tacgcagcag agctgcctgg ttttgggcga gtttcagaaa ggtaaggaat
     5161 attactttac tgaaaaattt tttatggagc ctcaaatcat tcaggtttat gcattacaac
     5221 aggcgatgga acagctggca ggcagacgga aggatctggc ggctgttcac tatgagaagg
     5281 ttctggaact ttatgaaatg cagactggaa gacggatcag ccttccttat cacatggaag
     5341 ccagaagaga ttacgaggga gtaagacttt gtcggtacgg agaggagaag tcagccggga
     5401 tgatcggtga aaagcataaa ggttcagata tccaaaacgg aaaaacagtt tgcggaaaaa
     5461 atatatcaga cagggaatgg aaaatcagaa ttccggggac tgtgaccagt ccgctgggaa
     5521 ttttttcggc agaaatattt ttgtatgaag gtcagaagat tgaagaaaaa aagtatacga
     5581 aatggatgga ttatgataaa ataaaaaaga atccgtatat tcgcaccaga cggacaggag
     5641 attatatggt tatcaatgcg caggggaaca caaagaagct gaaccgctgt atgatcgatg
     5701 agaaaatccc gtcggaatac agagacagca ttccgctgat cgcatgtgga aaagaaatcc
     5761 tctggatggt gggaagcagg atgaatgaga ggtataaaat taaccccaaa accaggaagg
     5821 tgttagtatt aaattaccaa ggaggaaacg aaaatgagtg agaaaatcaa agttttactc
     5881 agcgaagagg aagtagattc ccgtattaag cagatcgcag caaaggtcag caaggattat
     5941 gcaggcaagg aaatccattt aatctgtgta ttaaaaggtg gtgtattctt tacctgtgaa
     6001 cttgcaaaga gaatcacagt gccggtttct cttgacttta tgtctgtatc cagctatggt
     6061 gatgacacca agtccagcgg tgttgtaaag atcgtcaaag accttgacca gccactggaa
     6121 ggaaaggacg ttctgatcgt agaggatatc attgactcag gaagaacatt aagttatctc
     6181 atcgaaattc tgaagcagag aaatccaaac agcatccgtc tgtgtacatt gctggacaaa
     6241 ccggagcgaa gagttagaga tgtaagagtt gattattgct gctttaacat cccggatgag
     6301 tttgtagttg gctatggtct ggactatgca cagaaataca ggaatcttcc attcatagga
     6361 gtagtggaac tgggggaaga ctgaaaggag atttaagtaa gtgaataaac agccaagcag
     6421 aattggtctg tatctggtgc tgatcattgc gctggtagcc ggttattttt atctgaataa
     6481 ccaggtggtt tcccagagta attatacgca gaagcagctg gagcaggctc ttgaagacaa
     6541 caaggtggtt gatgcgacaa ttcagcagaa cagagaagtg cctacgggct ctgtgatcgt
     6601 agatactact gactccggtc agaagaaagt ctatgtgaca gatgtaaatc aggcaatcga
     6661 actgttgaat aaatacgata ttgatattac aactcaggat gtacccggag acaatgtatt
     6721 tctgacaacc ttgcttccgg ttcttctcac aggtgccctt gtgatctttc tgatcatgat
     6781 gatgaaccgc cagatggcag gtggcggtgg cggcggaaat gcaaagatga tgaatttcgg
     6841 caagagccgc gcaagaatgt catctccgga tgataataag aaagttacct ttgacaaggt
     6901 agcaggtctg caggaggaga aggaagatct ggcagaggtg gtagatttcc tgaaatcccc
     6961 acagaaatat acaaaagtag gcgcaaggat cccgaaaggt gtgctgcttg tgggacctcc
     7021 cggaacaggt aagacacttt tggcaaaggc agtggcagga gaagcgggag ttccgttttt
     7081 ctctatttcc ggttctgact ttgtggaaat gtttgtgggt gtaggtgcat cacgtgtgcg
     7141 tgatcttttt gaagaaggca aacgccatgc accatgtatt attttcattg atgagatcga
     7201 tgcggttgca aggcagcgtg gaaccggtat gggtggtgga catgatgaga gagagcagac
     7261 gctgaaccag cttctggtag agatggatgg ctttggtgtg aatgaaggga tcattgtgat
     7321 ggcagcgacg aaccgcgtgg atatcctgga tcctgcaatc cttcgtccgg gacgttttga
     7381 ccgtaaggtt gcagtgggac gtccggatgt gaagggaaga gaagagatcc tccgcgttca
     7441 tgccaaggat aaacctcttg gtgaggatgt agatctgact cagattgcca gaacaacagc
     7501 aggatttaca ggtgcagacc tggagaatct tctgaatgaa gctgccattg aagctgcaag
     7561 acggggaagg ggatttatcc ttcagtctga tatcaaaggt gcttttatca aagtaggcat
     7621 cggtgcagaa aagaagagta aggtgatctc tgagaaagag aaaaagatca ccgcgtatca
     7681 tgaatccgga catgcgatcc tgtttcatgt acttccggat atggatcctg tctatacgat
     7741 ttccattatc ccgacaggaa tgggagcagc ag
//
LOCUS       DBMDXG010000050         7722 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2491_length_7722_cov_1.716048, whole genome shotgun sequence.
ACCESSION   DBMDXG010000050 DBMDXG010000000
VERSION     DBMDXG010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2491_length_7722_cov_1.716048"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            206..760
                     /locus_tag="ACZPD3_02655"
     CDS             206..760
                     /locus_tag="ACZPD3_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR1414941.1"
                     /translation="MSSSIVIALPKIEDAKKIRTILNRHGLTVASVCSSVSNALASIS
                     ELDSGVLICGYKLTDAYYKDALDDLPQYFEMLLLASQRIIDEAPNSVSTVQMPMKASA
                     LIDTVNDMLYHLERRIKKEKKKPKPRSEKEQNYISNAKRLLMEKNQMTEEEAYRHIQK
                     CSMDSGTNMVETAQMLLMLMYDEI"
     gene            1135..2466
                     /gene="aspS"
                     /locus_tag="ACZPD3_02660"
     CDS             1135..2466
                     /gene="aspS"
                     /locus_tag="ACZPD3_02660"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIR1414942.1"
                     /translation="MEFMDGTWKKEINTWEELVGNNLLNQEAVIEGAVHSIRNMGDVA
                     FIILRRREGLFQTVYENEMANVSIHELKEAMTIRVKGILHEEERAPHGRELRIRHIDI
                     LSTPAEPLPMAIDKWKLNTSLEAKLNYRPISLRNIQERSKFKIQEALTKAFRDYLYGQ
                     GFTEIHTPKIGARGAEGGANLFKFSYFHKPAVLAQSPQFYKQMMVGVFDRVFETGPVF
                     RAEKHNTKRHLNEYTSLDFEMGYIDSFEEIMAMETGFLQYAMNLLKTEYAKEVQILKL
                     EIPDVSKIPAVRFDVAKELVSQKYNRKIRNPFDLEPEEEALIGQYFKEEYGSDFVFVT
                     HYPSKKRPFYAMDDPEDARFTLSFDLLFKGLEITTGGQRIHDYNMLVQKIEDRGMTQE
                     GMEQYLDTFKHGMPPHGGLGIGLERLTMQLIGEENVRETCLFPRDMNRLEP"
     gene            2526..2819
                     /gene="gatC"
                     /locus_tag="ACZPD3_02665"
     CDS             2526..2819
                     /gene="gatC"
                     /locus_tag="ACZPD3_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR1414943.1"
                     /translation="MAKIIDDETMENVCILSKLSLSEEEKEKAKAEMQKMLDYVEKLD
                     ELDTSEVEPMSHIFQDENVFREDVVTNGDNKEAMLANAPKAKEGQYQVPKTIG"
     gene            2831..4300
                     /gene="gatA"
                     /locus_tag="ACZPD3_02670"
     CDS             2831..4300
                     /gene="gatA"
                     /locus_tag="ACZPD3_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR1414944.1"
                     /translation="MELEKLTALQLGEKIKQRQVSVLDGVKTVFEQIEKQDSEVHAYL
                     DTYKEEAYKRAEEVQKGIEDGTYTSPLAGVPIAIKDNICINGKKTTCASKILENFVPQ
                     YNAEVIDRLEKAGLVIIGKTNMDEFAMGSTTETSAYGITRNPWNLEHVPGGSSGGSCA
                     AVAAGETYLALGSDTGGSIRQPSSYCGVTGIKPTYGTVSRYGLVAYASSLDQIGPVGK
                     DVSDCAALLEIIAGHDTKDSTSMKREDLQFSKELTGDIKGMKFGVPEEYLAEGLDPEV
                     KASFMGVLDTLKELGAEVEFFSIKTMEYMIPAYYIIASAEASSNLERFDGVKYGFRAA
                     EYEGLHDMYKKTRTAGFGEEVKRRIMLGSFVLSSGYYDAYYLKALRTKALIKKEFDQA
                     FGKYDVLLAPASPFTAPKIGESLKDPLAMYLGDIYTVAVNLCGLPGITVPCGKDSKGL
                     PIGIQMIGDCFMEKKILRAAHAYETSRGSFAVPGEGGTR"
     gene            4297..5739
                     /gene="gatB"
                     /locus_tag="ACZPD3_02675"
     CDS             4297..5739
                     /gene="gatB"
                     /locus_tag="ACZPD3_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR1414945.1"
                     /translation="MMKQYETVIGLEVHVELATKTKIFCGCSTEFGGAPNTHTCPVCT
                     GMPGSLPVLNKKVVEFALKAGLAANCQIHQYCKFDRKNYFYPDNPQNYQISQLYLPIC
                     HDGWVEIETSAGKKKIRIHEMHMEEDAGKLIHDEWEDCSLVDYNRSGVPLIEIVSEPD
                     MRSSEEVIAYLEKLRCMMQYLGVSDCKLQEGSMRADVNLSVREVGAEEFGTRTEMKNL
                     NSFKAIARAIEGERERQIELIEEGKAVTQETRRWDDNKEYSYAMRSKEDAKDYRYFPD
                     PDLPPIHISDAWIEKIKAEQPELREVKQARYQEEYGLPAYDAGILTESRHLAGLFEET
                     AAIYGNAKKTANWFMGEVLRLTKDKAMDPEQVSFSPKHLADLLVMVEKSEVSPQNAKK
                     VFEKVFEEDIDPVAYIEEHGLKIVEDTGLLEETINRILDANPGPLSELLGGKDKVMGF
                     FVGQIMKEMKGKANPASVRETLMKEVEKRK"
     gene            6161..7375
                     /locus_tag="ACZPD3_02680"
     CDS             6161..7375
                     /locus_tag="ACZPD3_02680"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR1414946.1"
                     /translation="MVTVNHNYLKLPGSYLFSTIGKKVKAYKEANPQANVISLGIGDV
                     TQPLAPAIIEALHKSVDEMGDAATFHGYAPDLGYEFLRSAIAKNDYKDRGCDIEADEI
                     FVSDGAKSDSGNIQEIFGLDNKIAVCDPVYPVYVDTNVMAGRTGEYNKERGNFDNVIY
                     MPCTEANGFLPEFPEEVPDLIYLCFPNNPTGGAITKPQLQEWVDYANKNGSVIIYDAA
                     YEAYISEENVPHSIYECEGARTCAIELRSFSKNAGFTGVRLGFTVVPKDLVRDGVDLH
                     SLWARRHGTKFNGAPYIVQRAGEAVYSPEGKAQLKEQVGYYMSNAKAIYEGLASAGYS
                     VSGGVNAPYIWLKTPDKMTSWEFFDYLLEKANIVGTPGSGFGAHGEGFFRLTAFGTHE
                     NTLEAIERIKNL"
BASE COUNT         2574 a         1553 c         1772 g         1823 t
ORIGIN      
        1 gcagtgagtg gggacttgtt tgtatcagga ggagcgaagc ttcttctgat aaactgcgaa
       61 gcagttattc ggttatgagc aagtagcgaa gcggattgcg aacatccgct tgatcaaaat
      121 caaacaagtc ccctgcggag gttgtaaaaa gcaataagca gtggacggaa actcaggctg
      181 taatagaaat aggagtggca gagttatgag cagcagcatt gtgattgcgt taccgaaaat
      241 tgaagacgca aagaaaatcc gcacaatact taacagacat gggctcacag tagcctctgt
      301 ctgcagctct gtctctaatg cgctggccag tatctcagaa cttgacagtg gtgtcctgat
      361 atgcgggtat aagctgacag atgcctatta taaagacgct ctcgatgacc tgccacagta
      421 ttttgaaatg ctgcttctgg cttctcagcg tattatagat gaagcaccga attccgtcag
      481 cactgtacag atgccgatga aagcaagcgc actgatagat acggtcaatg acatgctcta
      541 tcatctggaa cgccggataa aaaaagaaaa aaagaaacca aagcccagat cagaaaaaga
      601 acagaattat atctcgaatg ccaaacgcct tctgatggaa aaaaaccaga tgacagaaga
      661 ggaagcatat cgacacatcc agaaatgcag catggattca ggaaccaaca tggtagaaac
      721 tgcacagatg ttactcatgc tgatgtatga tgaaatctga tttttcaaac acgttctaaa
      781 taaaatatcc gaaaataata aggaaaattt tgtaaaaaat gacgatatta ttttgacaga
      841 atatgtggta atatacagaa tatatttttg ttcatatcaa gttagaggca aaatacatta
      901 tatatagaaa gaacacaaac aaacataaaa tagataataa caggaagaat gattacaaag
      961 gcgtaatcag agagtccagg ggataggtgt ctccggattt tctgattacg cctttttgat
     1021 attccagggt tttaagccaa aaagtcgttg tgcttgcacg aaaagccttt tgaacttgaa
     1081 accagtgaag atatcacaaa ggttttcaga aatgaagaaa tggaggatga ggaaatggaa
     1141 tttatggatg gaacatggaa aaaagaaatc aatacctggg aggaactcgt aggtaataat
     1201 ctcctgaacc aggaagccgt aatcgaaggc gctgttcaca gcatccgtaa tatgggtgat
     1261 gtggcattta tcattcttag aagaagggaa ggcttattcc agacagtcta tgaaaatgaa
     1321 atggcaaatg tttccattca tgaattgaag gaagcaatga cgatcagggt gaaaggaatc
     1381 ctgcatgaag aggagagagc accccacgga agagagcttc gtattcgtca tattgatatt
     1441 ttatcaacac cggcagagcc acttccaatg gcaatcgaca aatggaaact gaacacttcc
     1501 ctggaagcaa aattaaacta cagaccgatt tccctgagaa acattcagga gcgttccaaa
     1561 tttaaaattc aggaggcact gacaaaagca ttccgcgatt atctctacgg acagggattc
     1621 acagaaatcc acactccaaa gatcggagca agaggagcag agggtggagc aaaccttttc
     1681 aagttcagtt acttccacaa acctgcagta cttgcgcaga gcccacagtt ctacaaacag
     1741 atgatggttg gcgtatttga cagagtattt gagacaggcc ccgtattccg ggctgagaaa
     1801 cataatacca aacgtcactt aaatgaatac accagtctgg acttcgagat gggatatatt
     1861 gacagcttcg aagaaattat ggcaatggaa acaggattcc tccagtatgc catgaacctt
     1921 ctgaaaacag aatatgcaaa agaagtacag attttgaagc ttgagatccc ggatgtatcc
     1981 aaaatcccgg cagtccgttt cgatgtagcc aaggaactgg tatcacagaa gtataaccgt
     2041 aagatcagaa atccattcga tctggagccg gaagaagaag cactgatcgg acagtatttc
     2101 aaagaagaat atggctcaga tttcgtattt gtcacacatt atccgtcaaa gaaacgtcca
     2161 ttctatgcaa tggatgatcc ggaagacgca agatttacct taagctttga cctgttattc
     2221 aaaggccttg agatcacaac aggcggacag cgtatccatg attacaatat gctggtacag
     2281 aaaattgaag acagaggtat gacgcaggaa ggcatggaac agtatctgga taccttcaaa
     2341 catggtatgc cgcctcacgg tggactggga atcggtctgg aacgtttgac catgcaactt
     2401 attggtgaag agaatgttcg tgaaacctgt ctcttccctc gagatatgaa ccgtctggaa
     2461 ccgtaaataa gtcggaaata aaagcaatat agaatgttac aggcagtata tataaggaga
     2521 aaatcatggc aaagatcata gatgatgaaa caatggaaaa tgtctgcatc ctgtccaagc
     2581 tttccctctc agaagaagaa aaggaaaaag caaaagctga aatgcagaaa atgcttgatt
     2641 atgtagagaa actggatgaa ctggatacat cggaagtaga gcctatgtcc catattttcc
     2701 aggacgaaaa cgtatttcgt gaggacgttg tcacaaacgg agacaacaaa gaagcaatgc
     2761 tggcaaatgc accgaaggca aaagaaggac agtatcaggt tccgaaaacc ataggatagg
     2821 agacaggaca atggagttgg aaaaattaac agccctgcag cttggggaaa aaataaaaca
     2881 gcgtcaggtc agcgtcctgg atggagtaaa aactgttttt gaacagattg agaaacagga
     2941 ttcagaagta cacgcttatc ttgacacata taaagaagaa gcatacaaac gtgcagaaga
     3001 agtacaaaaa ggcatcgagg acggtactta taccagtcct cttgcaggcg taccgatcgc
     3061 gatcaaagat aatatctgta ttaatggaaa gaaaactacc tgtgcatcaa agattcttga
     3121 gaattttgtg cctcagtata atgcagaagt cattgaccgt ctggaaaaag ccggactggt
     3181 cattatcgga aaaacaaaca tggatgaatt tgccatggga agtacaacag aaacctccgc
     3241 ttacggcatc accagaaacc catggaatct ggaacatgta ccaggtggtt cttcaggtgg
     3301 ttcctgtgca gcagtggcag caggagaaac ttatctggca cttggttcag atacaggcgg
     3361 atctatccgt cagccaagtt cctactgtgg tgtaaccgga attaaaccaa cctatggaac
     3421 agtatcccgt tacggtcttg tagcttatgc atcttccctg gatcagatcg gtcctgtagg
     3481 aaaagacgta tctgactgtg cagctcttct ggagatcatc gcaggacatg atacaaaaga
     3541 cagcacatcc atgaaaagag aagatcttca gttctccaaa gaactgacag gagatatcaa
     3601 ggggatgaaa ttcggagttc ctgaagaata tctggcagag ggacttgatc cggaagtaaa
     3661 agcatctttt atgggtgttc ttgatacgct gaaagaactg ggagcagaag tagaattctt
     3721 ctctatcaaa accatggaat acatgattcc ggcttattat attattgcca gtgcagaagc
     3781 aagttccaac ctggaacgtt tcgatggggt gaaatatggt ttccgtgcag cggaatatga
     3841 aggtcttcac gacatgtaca aaaagaccag aacagcaggt tttggtgagg aagtaaaacg
     3901 ccgtatcatg cttggttcct tcgtgttaag ctccggttat tatgatgcat actatctgaa
     3961 ggcactccgc acaaaagccc tgatcaagaa agaatttgac caggcatttg gcaaatatga
     4021 tgtgctcctt gcacctgcat ctccatttac agcaccgaag atcggtgaaa gcttaaaaga
     4081 tccccttgcc atgtatcttg gagatatcta cacggtagca gttaatctct gcggtcttcc
     4141 gggaatcaca gttccatgtg gaaaagacag caaaggtctc ccaatcggta tccagatgat
     4201 cggggactgc ttcatggaaa agaaaatcct gcgtgcagca cacgcctatg aaaccagccg
     4261 cggctccttt gccgtgccag gagaaggagg caccagataa tgaaacagta cgaaacagtc
     4321 ataggacttg aagtccatgt agaacttgcc acaaaaacaa aaattttctg cggatgctcc
     4381 acagaattcg gaggagcacc caatacacat acatgtccgg tctgcacagg tatgccgggt
     4441 tccctgcctg tattaaataa gaaagtagta gagtttgccc tgaaagcagg tcttgcagca
     4501 aactgccaga tccatcagta ctgcaaattt gaccgtaaga actatttcta tccggacaac
     4561 ccacagaact atcagatctc tcagttatat ctgccaatct gtcatgacgg atgggtagag
     4621 attgaaacct ctgcaggcaa aaagaaaatc cgcatccatg aaatgcatat ggaagaggat
     4681 gcaggtaaac tgattcatga tgaatgggaa gactgctccc tggtagacta taaccgaagc
     4741 ggtgtcccgt tgatcgagat cgtatccgag ccggatatga gaagctctga ggaagtaatc
     4801 gcctatcttg aaaaactccg ttgtatgatg cagtatctgg gtgtatcaga ctgtaagctt
     4861 caggaaggct ccatgcgtgc agacgttaac ctttctgtcc gtgaagtggg agcagaagaa
     4921 ttcggtacca gaacagagat gaagaacctg aactctttca aagccattgc cagagcaatc
     4981 gaaggcgaaa gagaacgtca gatcgaactg atcgaagaag gcaaggcagt tacacaggag
     5041 acacgccgct gggatgacaa caaagaatat tcttacgcca tgcgttccaa ggaagatgcc
     5101 aaagactaca gatatttccc tgatccagat ctgcctccaa tccatatctc tgatgcatgg
     5161 attgaaaaga tcaaagcaga acagccggag cttcgcgaag tgaaacaggc ccgttatcag
     5221 gaagagtacg gacttccggc atacgatgcg ggaatcctta cagaatcccg ccatcttgca
     5281 ggcctttttg aagaaacagc agcaatctac ggaaatgcaa agaagacagc aaactggttt
     5341 atgggtgagg tactccgtct gacgaaagat aaagctatgg atccggaaca ggtatctttc
     5401 tcaccgaagc atcttgcaga tcttctggta atggtagaaa aatccgaagt cagccctcag
     5461 aatgccaaaa aagtctttga gaaagtcttt gaggaagaca ttgatcctgt agcctacatt
     5521 gaggaacatg gtctgaagat tgtagaagac acaggacttc tggaagaaac catcaacagg
     5581 atcctggatg ctaacccagg accactttca gaacttctgg gaggtaaaga taaagtcatg
     5641 ggcttctttg taggtcagat catgaaagag atgaaaggca aggcaaatcc tgcaagcgtc
     5701 cgcgagacac taatgaaaga agtagagaag cgcaaataaa ataaatcacc ccggacatta
     5761 tgcccggggt aaaaaatgtt actgttcact ctgttcacag caacacgctt cgcgatgcaa
     5821 aaaatatgaa cctccttgta caaagcacaa ggagtatcgt ttgcttcgca aacttaattt
     5881 atgctaatcg cataaattcg cacagtatgt ctcgggtttt ctgtgacctt cgctcacaaa
     5941 aaacacctcg cgggatatta ccagtgaaca gtaatgttaa tattaaaaat tatgctcaga
     6001 aaacactttg agactttaca ttattaaata attaagtata ataagattcg aataaaacat
     6061 atttgaagaa aagacacaaa tagaaatgga aaggcaaagc agcagcctgc tgtagacagg
     6121 tatataaaaa ctgcgaaaca tgccagggag gaaatcaaac atggtaacag taaaccacaa
     6181 ctatctgaaa ttaccgggaa gctatctgtt ttccacaatt ggaaaaaaag taaaagcata
     6241 taaagaagcc aacccacagg caaacgtgat ctcactggga atcggtgatg taacccagcc
     6301 tctggctccg gcgatcattg aggcacttca taaatctgta gatgaaatgg gtgatgcagc
     6361 tacatttcat ggttatgcac cagatcttgg ttatgaattc ttaagatctg ctattgcaaa
     6421 gaacgactac aaagacagag gctgtgacat tgaagcagat gaaatctttg tctctgacgg
     6481 tgcaaagagc gattccggaa atattcagga aatattcgga ctggataaca agattgcagt
     6541 gtgcgaccct gtatatcctg tatatgtaga tacaaacgta atggcaggtc gtacaggaga
     6601 atacaacaaa gaacgtggca actttgataa cgtaatctat atgccatgta cagaagccaa
     6661 cggcttcctt ccggaattcc cggaagaagt accggatctt atctaccttt gcttcccgaa
     6721 taacccaacc ggtggtgcca tcaccaaacc gcagcttcag gaatgggtag actatgcaaa
     6781 taaaaacggt tctgtaatta tctatgatgc agcatacgaa gcatacatct ccgaagaaaa
     6841 tgtaccgcac agcatctatg agtgcgaagg agcaagaacc tgcgccatcg aactgcgcag
     6901 cttctccaaa aatgcaggtt tcacaggtgt ccgtctcggc tttacagttg taccaaagga
     6961 tctggttcgt gacggtgtag atctccacag tctctgggca agacgtcatg gcacaaaatt
     7021 taatggtgcg ccatatatcg tacagcgtgc aggagaggct gtatactctc ctgaaggaaa
     7081 agcacagctg aaagaacagg taggttacta catgagcaac gccaaagcca tctacgaagg
     7141 tctggcatca gcaggctatt ccgtatccgg tggcgtaaac gctccatata tctggctgaa
     7201 aacacctgat aagatgactt cctgggagtt ctttgactat ctccttgaaa aagccaacat
     7261 cgtaggaact ccgggctcag gattcggtgc acacggtgag ggattcttcc gtctcactgc
     7321 gtttggaaca catgagaata cactggaagc aattgaacga attaagaatc tgtaagcagc
     7381 ttcctgatag gaacgagtgg gattataatt tcgcaaaatt taaccgaatc aagtaagttc
     7441 cctgcggagc agttattcgg tgtagagctg catagcaaag cgaatgattt tatccgcctg
     7501 actatgaaaa tgaaaaacgg caggacgaaa atccttgaaa atcaagggtt tcggacctgt
     7561 cgtttctatt ttataaaaat atatatggaa tttgattact atttaagtat taatgttgtt
     7621 ttctgagata atctgtcgaa tttgttcctt tgcaactgcc ataaaaaata ctccttttcg
     7681 ataagaattg tcatatctga attcttacca aaaaggagtt at
//
LOCUS       DBMDXG010000051         7720 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2492_length_7720_cov_1.902218, whole genome shotgun sequence.
ACCESSION   DBMDXG010000051 DBMDXG010000000
VERSION     DBMDXG010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7720)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7720)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7720
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_7720_cov_1.902218"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="ACZPD3_02685"
     CDS             <1..539
                     /locus_tag="ACZPD3_02685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR1414947.1"
                     /translation="LGMVGRQYFTKKHMDIAENFPYTVQKPTMHRARLISEEIVRAFL
                     DRELDEVRIFYTEMQSAVAMEPVNMQLLPLKKAEFLPNQAMLADMPQEEIVLSPSADV
                     LLNTMIPNYLTGLIYGCLVEAYASENNARMMAMQSSTDSAKKMLRELSIEYNRARQAA
                     ITQEITEIVSGAKAQKRK"
     gene            545..1942
                     /gene="atpD"
                     /locus_tag="ACZPD3_02690"
     CDS             545..1942
                     /gene="atpD"
                     /locus_tag="ACZPD3_02690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR1414948.1"
                     /translation="MNKGKIVQVMGPVVDVVFEDGNLPEIKDALEVENNGKRCVMEVS
                     HHLGNNMVRCIMLSASEGLQRDREVIATGSGIKVPVGDKTLGRLFNVLGDTVDDGPSL
                     EGEQKWVIHRDPPDFEHQKPAVEILETGIKVIDLLAPYAKGGKIGLFGGAGVGKTVLI
                     QELIQNIATEHGGYSIFTGVGERSREGNDLWSEMKESGVLEKTALVFGQMNEPPGSRM
                     RVAETGLTMAEYFRDEEHSDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLATE
                     MGELQERIASTKDGSVTSVQAVYVPADDLTDPAPATTFAHLDATTVLSRKIVEQGIYP
                     AVDPLESTSRILEPDVVGEEHYEVARKVQEILQKYKELQDIIAILGMEELSEEDKSTV
                     FRARKIQKFLSQPFHVAENFTGIKGKYVPLKETIRGFKAIVDGEMDQYPENAFFNVGT
                     IEDVIEKAKTEMGQE"
     gene            1955..2368
                     /gene="atpC"
                     /locus_tag="ACZPD3_02695"
     CDS             1955..2368
                     /gene="atpC"
                     /locus_tag="ACZPD3_02695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR1414949.1"
                     /translation="MADTFGLEIYASNKLAFAGRAKTLTIPAVDGEQAFLAQHENIVA
                     AIIPGEMRFEEADGTKHVLAVSSGFVEMINNRVKLFCLTAESPEEIDIRRAQEAKERA
                     EEQMRQKQSIQEYHMNQLALSRAMARLRVTSHKEI"
     gene            complement(2480..3991)
                     /locus_tag="ACZPD3_02700"
     CDS             complement(2480..3991)
                     /locus_tag="ACZPD3_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1414950.1"
                     /translation="MVHFFDITKFNLYKEDNRREVKKANGGLPSSLWETYSAFANCYG
                     GVIILGVAENKDGTWRTTGLKSTDRDKLLKHFWDTINNRKKVNVNLLSDQDVEIYEKD
                     EDTIIVIYVPMANREQKPVYINDDIFGGTFRRNHEGDYHCTKLQVKAMLRDQTDNTMD
                     MDVLDDVPISDLNYETIQGYRNRHRALKPAHPFGRLNDSEYLRSIGAAAISNIDKCLH
                     PTAAGMLMFGDEYNIVRHFPEYFLDYREILDPTIRWTDRLQSSSGEWSGNICDFYFRV
                     YNKLVKDIKVPFKTIDGNRIDDTPVHEALREALANCLINADFYGVRGIVVRKEADRIV
                     FENPGYSRTGKQQMKKGGISDPRNKVLMKMFNLINIGERAGSGVPNIFNTWEDQGWVE
                     PVIEEQFDPDRTLLILSFDKKQAIKTSDKKQAIKTSDKKQANKSCHKKQNQKTLENIE
                     KIREYLSKNNISKTSDIAEYIGLSLPRTRAILKEIPDVSPIGNNSNRKWTLQK"
     gene            complement(4114..5091)
                     /gene="bsh"
                     /locus_tag="ACZPD3_02705"
     CDS             complement(4114..5091)
                     /gene="bsh"
                     /locus_tag="ACZPD3_02705"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIR1414951.1"
                     /translation="MCTAATYKTKDFYFGRTLDYEFSYGDEITITPRNYPFHFRHSDK
                     APDSHYAIIGMAHVAGDYPLYYDAINEKGLGMAGLNFVGNAVYAEPVSDKENVAQFEF
                     IPWILSQCATLKEARQRLSTMNLTNTQFSENFPCAQLHWILADASGCLVIESMQDGLH
                     IYENPVGVLTNNPPFPQQMFQLNNYQSLSPRQPENTFAPGLELQSYSRGMGALGLPGD
                     LSSASRFAKVAFTKMNSRSGDSELESVSQFFHILGSVDQQRGCCEVAKGKYEITLYTS
                     CCNTTKGIYYYTTYENHQINAVDMHRENLDTTVLIRYPVLAKQNICFQN"
     gene            5361..>7371
                     /gene="yicI"
                     /locus_tag="ACZPD3_02710"
     CDS             5361..>7371
                     /gene="yicI"
                     /locus_tag="ACZPD3_02710"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: protein motif:HMM:NF007940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HIR1414952.1"
                     /translation="MKFTEGYWEKNERANASYAMQAFTAEAIPGGMRIVSPFRQITDR
                     GGALDVGTITTEFISVRKDIISVRSYHYEGYVKGEPRYELNEDPQETEVEITEDEAVM
                     KAGRMTVRVDLKDFKITYEADGKVLTNIGFRNLGYMQYDRATLTKFPEPNYMAAQYQP
                     YMVTELSLAPGECVYGLGERFTAFVKNGQVVDIWNEDGGTASQISYKNIPFYVTSKHY
                     GVFVDHSDHVSFEVASEKVENVGFSVKGEEIRYHIIYGDDIKGVIENYTDLTGKPALP
                     PAWSFGLWLSTSFTTNYDEETTNSFIQGMADRDIPLSVFHFDCFWMKEFHWCDFEWDS
                     RIFPDVPGMLKRYKDKGLKICVWINPYIAQGTNFFKEGLKNGYLVQRADGRGIKQIDN
                     WQPGMGLVDFTNPDAVKWYQNKLKTLLDMGVDCFKTDFGERIPVDVKYYDGSDPVSMH
                     NYYTYLYNKAVFDLLKEVKGEGEAILFARSATAGGQQFPVHWGGDCSATYASMAESLR
                     GGLSFTLSGFSFWSHDMGGFEMTAAPDVYKRWLQFGLLSTHSRLHGSKSYRVPWLFDE
                     EAVDVCRKFTKLKLRLLPYLYSMAVKSHKTGIPSMRAMIMEFNDDPATKYLDMQYMLG
                     DSILVAPIFNKEGHAEYYLPAGKWTHLLSGEVKEGGRWYEEDYDFF"
     assembly_gap    7372..7381
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7382..>7720
                     /locus_tag="ACZPD3_02715"
     CDS             <7382..>7720
                     /locus_tag="ACZPD3_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HIR1414953.1"
                     /translation="PVFVRENTLLPIGAVDTTVDYELEKDVQIQVYEVNETASCEVVT
                     RKGETAFTVKAVREGNKLTLEASAENGGMTYLLRNIHEIADVTGGSIVKDTENGIIIV
                     PEGCRQEIEL"
BASE COUNT         2195 a         1417 c         1861 g         2237 t
ORIGIN      
        1 tgctgggtat ggtaggccgt cagtatttca cgaagaaaca tatggatatc gcagagaact
       61 ttccatatac agtacagaaa ccgaccatgc acagagcgcg tctgatctca gaggagattg
      121 tcagagcgtt cttggacaga gaactggacg aagtccgtat tttctatacg gagatgcaga
      181 gtgcagttgc catggagccg gtgaatatgc agcttcttcc attgaagaaa gcagagttcc
      241 tgccaaatca ggcaatgctt gcggacatgc cacaggagga aatcgtgctg tcaccatcag
      301 ccgatgtact tctgaatacc atgatcccaa attacctgac tggtctgatc tatggatgtc
      361 ttgtagaagc ctatgccagt gaaaataatg ccagaatgat ggcaatgcag tcctctacgg
      421 acagtgcaaa gaagatgctc agggagttat ccattgagta taaccgtgcg cgtcaggctg
      481 ccatcacaca ggaaatcact gagattgtca gcggtgcaaa agcacaaaaa aggaagtgac
      541 gacgatgaac aaaggtaaaa ttgtacaggt tatgggacct gttgtagacg ttgtttttga
      601 agatggaaat cttcctgaga tcaaggatgc ccttgaggta gagaacaacg gtaaaagatg
      661 tgtaatggaa gtttcccatc atctgggaaa caatatggta cgctgcatta tgctgagcgc
      721 aagcgaaggc ttacagcgtg acagagaagt catcgccaca ggaagcggca ttaaggttcc
      781 tgtaggtgat aagacactgg gacgtttatt taacgtactt ggtgatacag tagatgacgg
      841 tccttccctt gagggagaac agaaatgggt catccacaga gatccgccgg atttcgaaca
      901 tcagaaaccg gctgtagaaa tcctggaaac aggtattaag gttatcgacc ttcttgcacc
      961 atatgcaaaa ggtggtaaga tcggtctgtt cggtggtgcc ggtgttggta agacagtcct
     1021 gattcaggag ctgatccaga acattgccac agagcatggc ggatattcca ttttcacagg
     1081 tgttggagag cgttcccgtg aaggtaacga cttatggtca gaaatgaaag agtccggtgt
     1141 tcttgaaaaa acagccttag tgttcggaca gatgaacgag ccaccgggat cccgtatgcg
     1201 tgttgctgaa accggactga caatggctga gtacttcaga gatgaggaac acagcgatgt
     1261 gcttcttttc attgataata ttttccgttt tgtacaggct ggttccgagg tttccgccct
     1321 tcttggacgt atgccttcag ccgtaggtta tcagccaaca ctggctactg agatgggtga
     1381 acttcaggaa cgtatcgctt ctactaagga tggttctgta acatctgtac aggctgtcta
     1441 tgtacctgcc gatgacctga ctgacccggc tcctgcaact acatttgctc atctggatgc
     1501 aacaaccgtt ctttccagaa agatcgtaga gcagggtatt tatccggccg ttgacccgtt
     1561 ggaatctact tcccgtatcc tggagccgga tgttgtaggt gaagaacatt atgaagtagc
     1621 acgtaaagta caggaaatcc ttcagaaata taaagaactt caggacatca ttgctattct
     1681 tggtatggaa gagctttctg aagaggataa gagcactgta ttccgtgcaa gaaagatcca
     1741 gaagttcctt tctcagccgt tccatgtagc tgagaacttc acaggtatca agggtaaata
     1801 tgttcctctg aaagaaacca tccgtggatt caaggcaatc gttgatggtg agatggatca
     1861 gtatccggaa aatgctttct tcaatgttgg tacaattgag gatgtcatcg agaaagcaaa
     1921 gactgagatg ggtcaggaat aaggagcgga ttttatggca gatacatttg gtcttgagat
     1981 ttatgccagt aataagctgg cgtttgcagg acgtgccaaa accctgacaa tcccggctgt
     2041 tgacggtgag caggcctttc tggcacagca tgagaatatt gttgctgcta tcattccggg
     2101 agaaatgcgt ttcgaggaag ctgatggcac aaagcatgtt ctggctgtga gcagtgggtt
     2161 tgtggagatg atcaataacc gtgtgaagct gttctgtctg acggcagaga gtcctgagga
     2221 gattgatatc cgcagggcac aggaagcgaa ggagagagca gaagagcaga tgagacagaa
     2281 gcagagtatt caggagtatc atatgaatca gttggctctg tccagagcta tggcacgact
     2341 tagggttacg agtcataaag aaatttaaag aaacaaaata tatcaataaa aagcatcttt
     2401 ggatggtctg atgaaggctt tccgtagatg cttttattgc tttgtaccat gttctgactt
     2461 atttcttggc tggtgaaaat tatttttgca gagtccattt tctgtttgaa ttatttccaa
     2521 ttggagatac atcaggtatt tcttttaaaa tagcccgggt tcgcggaaga cttaatccaa
     2581 tatattcagc aatatcactg gtttttgaga tattgttttt agataaatac tctctgattt
     2641 tttctatatt ttccaaagtt ttttggtttt gttttttatg acaggattta ttcgcttgtt
     2701 ttttatcgct tgtttttatc gcttgttttt tatcgcttgt ttttatcgct tgttttttat
     2761 cgaagctaag aatcagaaga gttctatctg gatcaaattg ttcttctata actggttcaa
     2821 cccagccttg atcctcccat gtgttaaaaa tgttgggtac tccgctgccg gcacgctcgc
     2881 cgatattgat cagattaaac attttcatga gtactttgtt gcgaggatca gaaattcccc
     2941 cttttttcat ttgttgtttt cctgttctgg aatatcctgg attttcaaat actattctgt
     3001 cagcttcttt tcggactaca atgcctcgaa caccataaaa gtctgcattt atcaaacagt
     3061 ttgcgagggc ttccctcaat gcttcatgta cgggagtatc atcaatacgg tttccatcga
     3121 ttgttttaaa aggtaccttg atatctttaa caagtttatt atatactcgg aagtaaaaat
     3181 cacatatatt accggaccat tcaccggaac ttgactgtaa gcgatcggtc cagcgtatag
     3241 ttggatccag aatttcacga taatctaaaa aatattcagg gaaatgtcgt acaatgttat
     3301 attcatctcc aaacattagc attcctgcag ctgttggatg aaggcattta tcaatattgg
     3361 aaatggcagc agctcctatg ctgcgtaaat attcagaatc atttaaacgg ccaaatggat
     3421 gtgcaggttt aagggctcga tgtctgttgc gatatccctg aatggtttca taatttaggt
     3481 cgcttattgg aacatcatcc agtacatcca tatccatagt gttgtctgtc tggtcgcgga
     3541 gcatggcttt cacctgaagt ttggtacaat gatagtctcc ttcgtgattt cttcgaaatg
     3601 ttccgccaaa tatatcatca ttaatataca caggtttttg ttctctgttt gccataggaa
     3661 cataaatcac gatgatagta tcttcatcct tctcatatat ctctacatcc tgatcagata
     3721 gaagatttac gttgaccttt tttcgattat tgattgtatc ccagaaatgt tttaacagct
     3781 tgtccctgtc tgtacttttt aaacctgtag ttctccaggt tccatctttg ttttcggcaa
     3841 ctcccagaat aattacgccg ccataacaat ttgcaaaagc agagtaagtc tcccataaag
     3901 aagaaggaag gccgccattt gcttttttta cttcgcgccg gttatcttct ttatataagt
     3961 tgaactttgt aatatcaaaa aaatgcacca ttgttattac ctctttttga aaccaataat
     4021 ttgtctttat tataccgtac agaactgaaa aatggcaatg gctataatta aagaatattt
     4081 gtcgaaattg ccacaggtat agagtgcttc acattaattc tggaaacaga tattctgttt
     4141 tgcgagtaca ggatatcgga ttaggactgt tgtatccaga ttttcacgat gcatatcgac
     4201 agcattgatc tgatgatttt cataggtggt ataataataa attccctttg ttgtgttgca
     4261 gcaggaagtg tagagagtga tctcatattt gccttttgct acttcgcagc agccacgctg
     4321 ttgatctacg gagccgagga tgtggaagaa ctggctgacg ctttcgagtt cggagtcgcc
     4381 ggatctggag ttcatttttg tgaaggcaac tttggcaaag cgggaggcgg aggaaaggtc
     4441 tcccgggagg ccgagggcgc ccataccgcg gctgtaggat tggagttcta atcctggagc
     4501 gaaggtgttt tctggttggc gaggggagag agactggtag ttgttaagct ggaacatctg
     4561 ctgtgggaag ggtgggttat tggttagtac ccctacagga ttttcgtaga tatggaggcc
     4621 gtcctgcatg gattcgataa cgaggcagcc ggaagcatct gcgaggatcc agtggagctg
     4681 agcacatggg aagttctcac tgaactgtgt gttggtgaga ttcatagttg agagtctctg
     4741 gcgggcttct tttaaggtgg cacactggct gagaatccat gggatgaatt cgaactgggc
     4801 tacgttttct ttgtccgata ctggttctgc atagactgca tttcctacga aattcagacc
     4861 tgccatgccg agaccttttt cattaatggc atcatagtag agtggatagt ctccggccac
     4921 atgagccatt ccgatgatgg catagtgaga gtcaggggct ttatcagaat gacggaaatg
     4981 gaatgggtag tttcgtggtg tgatggtgat ttcatcaccg taggaaaatt cataatcaag
     5041 ggttctgccg aaatagaaat cttttgtttt ataagttgct gctgtgcaca tattttctta
     5101 cctcctggtg ccaaaataga tatacttgta gaacgtgtat attttgaaga atattaccac
     5161 aatcttttgt ttttatgcaa tatacagatt ttgttttttg cacagataaa atttgggtta
     5221 tattgctggt aagaaaaaaa atcatgatgt atactggtat cagataacta ttcagcagtc
     5281 cgttatcccg cgaggtactg aaagagtttc aatttcggaa aataagcaaa tcataagatt
     5341 aaaagcaagg aggatcagga atgaagttca cagaaggtta ctgggagaaa aatgaacgcg
     5401 caaacgcatc ctatgcgatg caggctttta ctgcggaagc aattccggga ggaatgagaa
     5461 ttgtttctcc attccgccag atcacagaca gaggcggtgc tctggatgtt ggaactatca
     5521 ctacagaatt tatttctgta cgtaaagata tcataagtgt aagaagttat cattatgaag
     5581 gttatgtaaa aggtgagcca aggtatgagc tgaatgagga tccacaggaa acagaggttg
     5641 agattacaga agatgaagct gtgatgaaag ccggaagaat gacagtaaga gtggacttga
     5701 aagactttaa gattacatat gaagcagacg gaaaagttct cacaaacatc ggattccgca
     5761 atcttggcta tatgcaatat gacagagcta cgctgacaaa attcccggaa ccgaactata
     5821 tggcagcaca gtatcagcca tatatggtga cagagctttc tcttgcacct ggagaatgtg
     5881 tatatggtct gggtgagaga tttacagcgt ttgtaaagaa cggtcaggtt gttgatatct
     5941 ggaatgaaga tggcggtaca gcttctcaga tttcttataa aaatattcca ttctatgtaa
     6001 caagcaaaca ttatggcgta ttcgtagacc acagtgatca tgtttccttc gaagttgcaa
     6061 gtgaaaaggt tgagaatgtg ggattctctg tgaaaggtga agagatcaga tatcatatta
     6121 tttacggaga tgacattaag ggtgtaattg agaactacac agatcttaca ggaaaaccgg
     6181 cattgccgcc tgcatggtcc tttggattat ggctttccac atcctttaca acaaactatg
     6241 atgaagagac gaccaattcc tttatccagg gaatggcaga cagagatatt ccattgagtg
     6301 tattccattt tgactgcttc tggatgaaag agttccactg gtgtgatttt gaatgggatt
     6361 ccagaatctt cccggatgta ccgggaatgc tgaaacgtta taaggataaa ggacttaaga
     6421 tctgtgtatg gattaatcca tatattgcac agggaactaa cttctttaaa gagggtctga
     6481 agaacggata cctggtacag cgtgcagacg gaagaggaat caagcaaatc gacaactggc
     6541 agcctggcat gggcctggtt gactttacaa atccggatgc agtgaagtgg tatcagaata
     6601 agctgaaaac tttgcttgat atgggtgttg actgtttcaa gacagacttt ggcgagagaa
     6661 ttccggtcga tgtgaaatat tatgacggtt cagatccggt ttctatgcat aattattata
     6721 cgtaccttta caacaaggca gtatttgatc tgctgaagga agtaaaaggt gaaggtgaag
     6781 caatcctttt tgcaagaagt gcgacagcag gcggacagca gttcccggtt cactggggcg
     6841 gtgactgttc tgcaacctat gcctccatgg ctgagagctt aagaggtggt ctgtccttta
     6901 ctttatcagg attttccttc tggagtcatg atatgggcgg atttgaaatg acagcagcac
     6961 cggatgtata caaacgctgg ctgcagtttg gtcttctttc cacacacagc cgtctccatg
     7021 gttccaagag ttatcgtgta ccgtggctgt ttgatgaaga agctgtagat gtatgccgta
     7081 agtttacaaa gctgaaatta agattactcc catatctgta ttctatggcc gtaaaatcac
     7141 acaagacagg tattccgtcc atgcgtgcaa tgatcatgga atttaacgat gatcctgcaa
     7201 ccaaatatct ggatatgcag tatatgttag gtgacagtat tctggttgca ccaatcttca
     7261 acaaagaagg tcatgcagaa tattatctgc cggcaggaaa atggactcat cttttaagcg
     7321 gagaagtcaa agaaggcgga agatggtatg aagaagatta tgattttttt tnnnnnnnnn
     7381 ntgcctgtat ttgtaagaga gaatactctg cttccgattg gtgctgtaga tactaccgtt
     7441 gattatgaac tggagaaaga tgtacagatt caggtttatg aagtaaatga aactgcatcc
     7501 tgtgaggtag tgacgcgtaa aggagagaca gcttttacag taaaagctgt aagagaggga
     7561 aataaactca ctctggaagc ttcagcagaa aatggaggta tgacctatct gctgagaaat
     7621 attcatgaaa tagccgatgt tactggtgga agcatagtga aagatacaga aaatggtatt
     7681 atcattgtac cggaaggatg cagacaggaa atcgaactct
//
LOCUS       DBMDXG010000052         7698 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2498_length_7698_cov_2.236409, whole genome shotgun sequence.
ACCESSION   DBMDXG010000052 DBMDXG010000000
VERSION     DBMDXG010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2498_length_7698_cov_2.236409"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            17..712
                     /locus_tag="ACZPD3_02720"
     CDS             17..712
                     /locus_tag="ACZPD3_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1414954.1"
                     /translation="MRICGFNKTTLLDYPGKVASTIFLGGCNFRCPFCQNGILVVAPG
                     EQPDYSQEELLTFLKKRKGILDGVCISGGEPTLSDGLEEFLGKIKELGYAVKLDTNGS
                     RPKIVKHLAEAGLIDKVAMDIKACPDNYGNLTGIEKPDMDSIFETADFLLHGNLDYEF
                     RTTVVRELHTQKDFEEIAGWLAGAKEYYLQAYKDSDGVLRPGYGSYTFEELQNFQKIL
                     QKTILSVGIRGID"
     gene            complement(683..1726)
                     /locus_tag="ACZPD3_02725"
     CDS             complement(683..1726)
                     /locus_tag="ACZPD3_02725"
                     /inference="COORDINATES: protein motif:HMM:NF040001.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
                     /protein_id="HIR1414955.1"
                     /translation="MAGYRRFIAYVYDYESGKKGSNCGFIKVEVKDQQCSVEIHLHCP
                     GLPENVKCNIYGFTRKDGLINGILLDTCETEKETVECLIITDATDMNDSGVAMGKLGG
                     MIITSDTGGFFGTEWDDQPIRPENFHEIKAMPDADIPESAKITSQKPEVPADMVLQEM
                     PEELEDISEKPEDTSLTKVPDDSLSIEPSANIPAQGTSDNKPGTESSYISDNTVEDSN
                     SSENAGNPVPERNAENRKTSAEFSPFSDGELISAWKIHLDDLKHFPRHYCALRNNRFL
                     QYGHYNFGYLLLGQRNNGQYILGVPGVYNQQERFMANMFGFPYFKESSYIDIPKMRGG
                     YWYRLIDAPDSHR"
     gene            1859..1932
                     /locus_tag="ACZPD3_02730"
     tRNA            1859..1932
                     /locus_tag="ACZPD3_02730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1893..1895,aa:Pro,seq:cgg)
     gene            2022..2537
                     /gene="tadA"
                     /locus_tag="ACZPD3_02735"
     CDS             2022..2537
                     /gene="tadA"
                     /locus_tag="ACZPD3_02735"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR1414956.1"
                     /translation="MTDQERFMKEAIRQAKKAEALEEVPIGCVIVHEGKIIARGYNRR
                     NTDKNTLSHAELNAIRKASKKLGDWRLEGCTMYVTLEPCQMCSGALVQSRIDEVVIGC
                     MNAKAGCAGSVMNLLQVDGFNHQVKITQGVLEEECSSMLSEFFRKLREKKKQEKAALK
                     AAQENPEGEPE"
     gene            2646..4013
                     /locus_tag="ACZPD3_02740"
     CDS             2646..4013
                     /locus_tag="ACZPD3_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1414957.1"
                     /translation="MKKDTTNDMTVGSPVSLIIKFMIPMCLGNLFQQFYNIADSIVAG
                     KFIGVNALAAIGSTGSLMFFVTGWLNGLSSGFAIIVAQMFGAKKFDRMRHYVAMSIYL
                     MAAFSIVMTIGFSLANKPILHLMNSPEEVFGDVTAYMGIIYAGLIITGAYNALAAFLR
                     ALGDSKSPLYFLIISAVINVILDVVFIVVFGMGVEGCGYATVIAQGISAVCCLIYIVK
                     RFPILHLERKDFEICWDSFGRLLKLGIPMGLQFSITAIGTIIVQGAVNIYGPVHMAGF
                     SAAGKIQNIFATVFTAFGATIATYVGQNRGAGKMDRVKQGVRYTQMMVLGWSVFVMFL
                     MFFFGKYLTYLFVDPSEQDVVNVSVTYFRTVFWAYPFLGSIFLYRNTLQGMGYGLVPM
                     LGGVFELVARTGIVVLIAGHTSFAGVCMADPTAWIAALIPLIPYYFHVMKKYKNKSQV
                     QAVED"
     gene            complement(4175..4436)
                     /gene="ffs"
                     /locus_tag="ACZPD3_02745"
     ncRNA           complement(4175..4436)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZPD3_02745"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4723..5805
                     /locus_tag="ACZPD3_02750"
     CDS             4723..5805
                     /locus_tag="ACZPD3_02750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1414958.1"
                     /translation="MIKRIGMLTSGGDCQALNATMRGVVKGLSNNLDELEVYGFDDGY
                     KGLIYGKYRMLTSKDFSGILTQGGTILGTSRQPFKLMRVPDENGLDKVEAMKQTYYKL
                     CLDCLVILGGNGTQKTANLLREEGLNIIHLPKTIDNDIYGTDMTFGFQSAVNIATNAI
                     DCIHTTASSHGRVFIVEIMGHKVGSLTLHAGVAGGADIILIPEIPYDIKKVTAAIQKR
                     AKAGKRFTILAVAEGAISKEDAELPKKKYKEKLEARAKKYPSVSYEIADQIYKEIGSE
                     VRVTVPGHTQRGGEPCPYDRVLSTRIGAGAAQAIMDGEYGIMIGVVNGKIKRVPLEEC
                     AGKLKMVSPKDQLVVAAKQIGISFGD"
     gene            5975..7594
                     /gene="dnaX"
                     /locus_tag="ACZPD3_02755"
     CDS             5975..7594
                     /gene="dnaX"
                     /locus_tag="ACZPD3_02755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR1414959.1"
                     /translation="MSYTALYRKFRPDNFADVKGQDHIVTTLTNQIKHNRIGHAYLFC
                     GTRGTGKTTVAKILAKAVNCEHPVNGSPCNECAMCKAIQAGTAMNVIEIDAASNNGVD
                     NIREIREEVSYRPTEGKYKVYIIDEVHMLSTGAFNALLKTLEEPPSYVMFILATTEAH
                     KIPITILSRCQRYDFHRITIDTIAARLDELLKVEGVEAEEKAVRYVAKAGDGSMRDAL
                     SLLDQCIAFYLGQELTYDKVLEVLGAVDTEVFSKLLRKVIRGDVTGSIHILEELIVGG
                     RELSQFVGDFTWYMRNLLLVKTSENPEEAIDVSSDNMKLLKEESTMLDVETLMRYIRI
                     FSDLSNQIRYATQKRVLVEIALIKLCRPAMETNLDSVLDRLRVLEQRMDERPVQQVIV
                     QQGSGKMPAETGAVQEPAGNKAPAKAAPEDLQKIVAGWRVITGQTTGMFKQMLQKSVP
                     KYNSETGEPVLYVEFQDFLGQSYVDNPEAKKELQDIITAQTGKTVEIQMLVADKHQHT
                     NLANITVDQAIKNNIHMDVVIEEDPDEEKGE"
BASE COUNT         2289 a         1506 c         1875 g         2028 t
ORIGIN      
        1 ggaatggaga taaacaatga gaatctgtgg ctttaataaa acgacattgc tggactatcc
       61 gggaaaggta gccagtacta tatttctggg tggatgcaat ttccgctgtc cgttctgtca
      121 gaatggtata ttggttgtgg ctcccggaga acagccggat tacagtcagg aggaactttt
      181 gacttttttg aaaaaaagaa aggggattct ggacggggtc tgcatttcag gaggggaacc
      241 tacactgtca gatggtctgg aggaatttct gggaaaaatc aaagagctgg gctatgctgt
      301 taaactggac accaatggct cacgtccgaa gattgtaaaa catctggcag aagccggact
      361 gatcgataaa gtagctatgg atatcaaagc ctgtccggat aactatggaa atctgaccgg
      421 aatagaaaag cctgatatgg acagtatttt tgagacagca gattttctgc tgcatgggaa
      481 tctggactat gaattcagaa caacagtagt acgggaactg catacgcaga aggattttga
      541 agaaatcgcc gggtggctgg caggagcgaa agaatattat ctgcaggctt ataaggactc
      601 agatggagtc ctcaggcctg gatatggaag ctatactttt gaagaacttc agaattttca
      661 gaagattctt caaaagacga ttttatctgt gggaatccgg ggcatcgatt aaacgatacc
      721 agtaaccgcc gcgcattttt ggaatatcta tatagctgct ttctttgaag taaggaaagc
      781 caaacatatt tgccataaag cgttcctgct gattataaac gccgggaacg ccgaggatat
      841 actgtccgtt atttctttgt cctaaaagca aatatccgaa attataatgc ccatactgga
      901 gaaaacggtt attgcgcagt gcacagtaat gccgcggaaa atgcttcagg tcatcaaggt
      961 gtatcttcca tgcagaaatc agttcgccat cggagaaggg ggaaaattcg gcagaggttt
     1021 ttctgttttc tgcatttctt tccgggacag gattgccggc attttcagaa ctattggaat
     1081 cttcaacagt attatctgag atataggatg actctgttcc tggcttgtta tcagatgttc
     1141 cctgagccgg aatgttggca gacggttcaa ttgataaaga atcgtcagga acttttgtaa
     1201 gcgaggtatc ttcgggtttt tctgaaatat cttcaagttc ttcgggcatt tcctgtaata
     1261 ccatatctgc cggaacttcg ggtttctgtg aagtgatttt cgcagattca ggaatatctg
     1321 cgtcaggcat agcttttatt tcatggaaat tctccggacg tataggctga tcgtcccatt
     1381 cagtaccaaa gaatccgccg gtatctgaag taatgatcat tccgcccaac ttacccattg
     1441 caacgccgga gtcattcata tctgttgcat ctgttatgat caggcattct acagtttctt
     1501 tttctgtctc gcaggtatcc agaagaattc cgttgatcag gccatccttt cgtgtaaatc
     1561 cataaatatt gcattttaca ttttcaggca gccccggaca atgcagatga atctcaacag
     1621 aacattgctg atcttttact tcaactttaa taaatccgca attgctgcct ttctttccgc
     1681 tttcatagtc atatacatat gcgataaaac gccgatatcc agccaaaatc cttcctccgt
     1741 aaaaccagat tcaataatgt atatgaccat cagaagaaaa tattcgaaaa aattaaaaaa
     1801 atttgaaaaa atctattgac aaactgcgat tttattaata taatagcact tgcattgacg
     1861 aagcgtggct cagtttggta gagcgctgcg ttcgggacgc agaggtcgtg ggttcgaatc
     1921 ccgtcgcttc gactacgcat aggaagtctg aaaccaattg gttttcaggc tttctttttg
     1981 ctattctggt agtgatgcaa cagcagaaag gaaaggaaaa gttgacagat caggaacgtt
     2041 ttatgaaaga agctatccgt caggcgaaga aggcagaagc tctggaagaa gtgccaatcg
     2101 gctgtgtgat cgtgcatgag ggtaagatca tcgcacgtgg atataacaga agaaacacag
     2161 ataaaaatac actctcccat gcagaactga atgcaatccg caaagccagt aaaaaacttg
     2221 gagactggcg gctggaggga tgcactatgt atgtgacgct ggaaccatgc cagatgtgct
     2281 ccggggcact tgtccagtcc agaatagatg aagtagtgat cggctgtatg aatgccaaag
     2341 cgggctgcgc aggttctgtg atgaatcttt tgcaggtgga tggatttaat catcaggtaa
     2401 agattacaca gggagttctg gaagaggaat gttcttctat gttatctgag tttttcagaa
     2461 agcttcgtga gaaaaagaag caggaaaagg cagcccttaa agctgcacag gaaaatccgg
     2521 agggagaacc ggaataataa aagtaaaaga cagacaaaaa caggaagaat cttaaaggca
     2581 ataagagtaa taaaaataac agaagtaatg ttacaggcag aaataaaaag aggcgaataa
     2641 aaagaatgaa aaaagataca acaaacgaca tgaccgtcgg cagtccggtg agtctgataa
     2701 taaaatttat gatcccaatg tgcctgggaa atctttttca gcagttttat aatattgcag
     2761 attccattgt tgccggaaaa tttatcggag taaatgccct tgcagctatc ggaagcaccg
     2821 gttcactgat gttttttgtg accggctggc tgaatggact cagcagtgga tttgcgatta
     2881 tcgtagccca gatgttcgga gcgaaaaaat ttgacaggat gcgccattat gttgccatgt
     2941 ccatttacct tatggctgca ttttccattg tcatgaccat aggattcagt cttgcgaaca
     3001 aaccaatcct gcatctgatg aattcaccgg aagaggtatt tggtgatgta actgcatata
     3061 tgggaattat ttatgcaggt cttattatca caggtgcata taatgcactt gcggctttcc
     3121 tgcgtgcgct gggtgactcc aagtcgccat tgtatttcct gatcatttca gctgtgatca
     3181 atgttattct ggatgtagtt tttattgtag tctttggaat gggtgtagaa ggatgcggat
     3241 atgccacagt gatcgcacag ggaatttcgg cagtgtgctg cctgatttat attgtaaaaa
     3301 gatttccgat ccttcatctg gaacggaagg attttgagat atgctgggac agtttcggca
     3361 gacttctgaa gcttggaatt cctatgggac tgcagttttc gattacagca atcggaacca
     3421 ttattgtaca gggcgcggtc aatatttacg gacctgtaca tatggccgga ttctctgctg
     3481 ccggtaagat ccagaatatt tttgccacag tatttacagc cttcggtgca accatagcaa
     3541 cctatgtagg tcagaacagg ggcgcaggaa aaatggatcg tgtgaaacag ggagtccgtt
     3601 acacgcagat gatggtcctg ggatggagcg tttttgttat gttcctgatg tttttcttcg
     3661 gaaaatatct gacatatctt ttcgtagatc catcggaaca ggacgtagta aatgtgtctg
     3721 ttacttattt ccggacagta ttctgggcat atccgtttct cggaagtatt ttcctctatc
     3781 gaaatactct gcagggaatg ggatatggac ttgtaccaat gcttggcggg gtatttgaac
     3841 tggtggcgag aaccggaatt gtcgtattga ttgcgggaca tacttccttt gccggtgtct
     3901 gtatggctga cccgaccgca tggatcgccg cactgatccc actgattcct tattattttc
     3961 atgtgatgaa gaaatataag aacaaaagtc aggtacaggc agtagaggat taattattct
     4021 ttatctgatg gaaattgtat aaggctcttt cctgcttctg ttaatatatg gacttttgta
     4081 aaccacaaaa ataagaaaag cattcagaaa attatcaaca gataaaattc tgaatgcttt
     4141 tttatatgat gacctgcatc atgaatattc ttttttgcgc gccgcacttc ggaatggtcc
     4201 cacagacgtt acctctacag ttaactcagc ccaggcaccc ttacggcaca caagagcttc
     4261 tgcttagtgc tgctacattc ctgtcctgac acggttcaca gattcctgtt gcgtaagacc
     4321 caaacgtcaa cgccacttat agagggcagc tccacaggat acaagcctcg gattggcatc
     4381 acccctgctg tagcggattg caggtacagg gcaccgctat ctccccggct gcgcatttct
     4441 gattatatag gcaggaggct gttttgtcaa tagtgaatca ttatagtaaa acttattact
     4501 gggaacggag tgaacagtaa caaacggagt gaacagtaac gaacgtagtg cgtgaatagt
     4561 aactaaaact tttctaacac tattgtatga aaacgataaa tattatataa taaacagaaa
     4621 taaactatct ttattctgga aatggatatg ccagagtaca caacatgtct tgtatgtatg
     4681 gatatggata attcttaaaa cacagaaatg gaggatacta tcatgattaa aagaataggt
     4741 atgcttacca gtggcggtga ctgtcaggct cttaacgcta ccatgcgagg agtagtaaag
     4801 ggactgagta acaatttgga tgaactggaa gtttatggat ttgatgatgg atataaagga
     4861 ctgatctatg gtaaataccg tatgctcaca tccaaagatt tttccggaat ccttacacag
     4921 ggcggcacaa tcctcggaac atccagacag ccattcaaac tgatgcgtgt cccggatgag
     4981 aatggcctgg acaaggttga agcaatgaag cagacttatt ataaactctg cctggactgc
     5041 cttgtgatcc ttggcggaaa tggtacacag aagacagcaa accttctgag agaagaagga
     5101 ctgaacatta tccatcttcc aaagactatt gataatgaca tctatggcac agacatgacc
     5161 tttggtttcc agagtgcggt aaacattgcc acaaatgcca ttgactgtat ccatacgaca
     5221 gcatcttcac atggacgtgt atttatcgtg gagatcatgg gacacaaggt aggaagtctg
     5281 acacttcatg caggtgttgc aggcggtgcg gatatcatcc tgattcctga gatcccatat
     5341 gatatcaaaa aagttaccgc agcgatccag aaacgtgcca aagcaggcaa gcgtttcaca
     5401 atccttgcag ttgcagaagg tgcgatttcc aaagaagacg cagaacttcc taaaaagaaa
     5461 tacaaagaga aactggaagc ccgagcaaag aaatatccgt ccgtttccta tgagattgct
     5521 gaccagatct acaaagagat cggaagcgaa gtccgcgtta cagttccggg acatacacag
     5581 agaggcggag aaccatgtcc gtatgaccgc gttctttcca caagaatcgg tgcaggtgca
     5641 gcgcaggcga tcatggatgg tgaatatggc atcatgatcg gcgtggtaaa cggcaaaatt
     5701 aaacgtgtac ctcttgaaga gtgtgcaggt aagcttaaga tggtatcacc gaaagatcag
     5761 cttgtagttg ctgccaaaca gatcggtatc agctttggtg actgattaag tgaaacagta
     5821 cttgactgac agatactttt tcatataaga tatggatgta aaaaagaccg ccgcgggaat
     5881 tttttccgcg gcggctgtga taataagata gagataacta tttggcgcgc tgctgacagt
     5941 gggggtgctg aatagttacg ataggagttt actcatgagt tacactgctc tttacagaaa
     6001 gttcagaccg gataatttcg cggatgtcaa aggtcaggat cacatagtga ctactctgac
     6061 aaatcagatt aagcataacc gtatcggcca tgcgtatctg ttctgcggca ccagaggaac
     6121 cggtaaaaca acagtagcca aaattctggc gaaggcagtc aactgtgagc atcccgtaaa
     6181 tggcagcccc tgtaatgaat gtgccatgtg caaggcaatc caggcaggaa ctgcaatgaa
     6241 tgtgatcgag atcgatgctg catccaacaa tggtgtggat aatatacgtg agatcagaga
     6301 agaggtttct taccgcccta cagaaggtaa atataaagta tatattattg atgaggttca
     6361 tatgctttcc acaggtgcct tcaatgccct gctgaaaaca ctggaggaac caccgtctta
     6421 tgttatgttt atcctggcaa ctacagaggc gcacaagatt ccgattacga ttttatccag
     6481 atgccagaga tatgatttcc acagaatcac catagacact atcgcggcca gactggatga
     6541 actgctcaaa gttgaaggtg tagaagctga ggaaaaagca gtccgatatg tagcgaaagc
     6601 gggagatggt tccatgcgag atgcgctcag ccttctcgac cagtgtattg ctttttatct
     6661 tggacaggaa cttacctatg ataaagttct ggaggttctg ggagccgttg acaccgaagt
     6721 gtttagcaag cttttgcgaa aagtgatccg gggagatgtg acaggttcca ttcatatcct
     6781 ggaggaactg atcgtaggag gcagggaact gagccagttt gtgggagatt ttacctggta
     6841 tatgagaaat cttcttctcg ttaagacttc tgagaatccg gaggaagcaa tcgatgtctc
     6901 atccgacaac atgaagcttc tgaaagaaga gagcaccatg ctggatgtgg agacgctgat
     6961 gcggtatatc cgtatttttt cggatttgtc caaccagatc cgatatgcaa cccagaaaag
     7021 ggttctcgtg gagattgcac tgatcaagtt gtgccgtcct gcaatggaga ctaatctgga
     7081 ttctgttctg gacagactgc gtgtgctgga acagcggatg gatgaacgtc cggtacagca
     7141 ggtgatcgtg cagcagggtt cagggaaaat gccggcagaa accggagcag ttcaggaacc
     7201 ggcgggaaac aaagcaccgg cgaaagctgc accggaagat ttgcagaaga tcgtggcagg
     7261 ctggagagtg atcacaggac agacaacagg gatgtttaaa cagatgcttc agaaatctgt
     7321 tccgaagtat aacagtgaga caggagaacc tgttctttat gtggaatttc aggattttct
     7381 ggggcagtcc tatgtggaca atccggaagc gaagaaagag cttcaggaca tcatcactgc
     7441 ccagacagga aaaacagtag agatccagat gctggtggca gataagcatc agcatacaaa
     7501 tctggcaaat atcacagtgg accaggcgat aaagaataat atccacatgg atgttgtgat
     7561 agaggaagat cctgatgagg aaaaaggaga ataaatattg ttactgttca cacaccacta
     7621 tcccgcgagg tgttttggca tgaatatgcc aaaatcccga gacatacaag ccaaaattcc
     7681 attgccgcag gcaatctg
//
LOCUS       DBMDXG010000053         7623 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2525_length_7623_cov_1.927280, whole genome shotgun sequence.
ACCESSION   DBMDXG010000053 DBMDXG010000000
VERSION     DBMDXG010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_7623_cov_1.927280"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      355..459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            546..1106
                     /locus_tag="ACZPD3_02760"
     CDS             546..1106
                     /locus_tag="ACZPD3_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIR1414960.1"
                     /translation="MQKKQYWNVKTLVFMALLIAMHLVLTRVLVIDLGAYRISVGSVC
                     TILAGLWLGPVAGGVCGLCADIIGCFMKGYAVNPFITVAAILWGVLPALAKPLFANRK
                     KTGKTVGICVSIVVTAVLSSLVLTTAGLVIMLGYNFYAIMPGRLVQFAIMIPIYCVLT
                     CLLYFSPLTAMVVGNTSPAVLKNKTV"
     gene            complement(1441..2217)
                     /locus_tag="ACZPD3_02765"
     CDS             complement(1441..2217)
                     /locus_tag="ACZPD3_02765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1414961.1"
                     /translation="METTIKQIEDMSLNAWPSHKMELYDGWILRFSYFYTHRTNSVEQ
                     FGNSTLTWREKIPYCESVYKRLGTPAVFKISPLVSPDFDYVLENRGYAIQHTTNVMAM
                     SMNAAHLDTPYPDVTFYNNIPSEWIESLFNLKNTINPIHRKVVPSMYQAILKETICAC
                     IRIDGQIIATGLGILDRDYIGIYAIHVKEEYRKHGYARQICTGLLKEGMKKGAQNAYL
                     QVVEGNDNARALYRSLGFQQLYTYWFRVQPDENGNFPPEK"
     gene            complement(2318..3013)
                     /locus_tag="ACZPD3_02770"
     CDS             complement(2318..3013)
                     /locus_tag="ACZPD3_02770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1414962.1"
                     /translation="MQIICLGDSITDCNHLFEDFPLGNGYVQILSEMFRNQTPSFSIS
                     ADTVRRSSSAVQLTKSTGAIHFRNCGIDGFTVARVLENIRQHRIFLHRSPVITLLIGI
                     NDIGLIMNTDRTDSQKEQMMREFATHYNELLNLLTADARQVILMEPFIFPHPEEYETW
                     IPYVHTMSDIIRQLSVRFRLPFLPLHNYFNKEATQSGFDAITTDGIHLTLYGHKLLAE
                     KLFPLLQNIDNNP"
     gene            3445..4107
                     /locus_tag="ACZPD3_02775"
     CDS             3445..4107
                     /locus_tag="ACZPD3_02775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR1414963.1"
                     /translation="MEELQCKTVFTIPVFGGIPVAESVAVTWVIMAVLLILSLVLVRN
                     LSVENPGKKQLLLETGVSFLHNFFKDILGEEGKMYIPYLMTVVIYIGIANIFGVFGFV
                     PPTKDLNCTIGLAITSIFLIEYAGFHKKGLKGFLKSFAEPAPIMLPINILEVVIRPTS
                     LCMRLFGNVLGSYVVMKLLEFICPAVLPIPFSLYFDFFDGFIQAYVFVFLTSLFIKEA
                     IE"
     gene            4369..4587
                     /gene="atpE"
                     /locus_tag="ACZPD3_02780"
     CDS             4369..4587
                     /gene="atpE"
                     /locus_tag="ACZPD3_02780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR1414964.1"
                     /translation="MLVAIGAGIAVLTGIGAGIGIGMATSKAVEAIARQPEAESKISK
                     SLLLGCALAEATAIYGFVIGLLIVIMLG"
     gene            4628..5128
                     /gene="atpF"
                     /locus_tag="ACZPD3_02785"
     CDS             4628..5128
                     /gene="atpF"
                     /locus_tag="ACZPD3_02785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR1414965.1"
                     /translation="MIEININLVFTIINLIVLYLLMKKFLFGPILNVMEQRKNMIDQQ
                     FASAKDTEEQAYELKGKYEDALKSAKDESMRIVNQAKDEAKVQAERIVKDANTQAGAM
                     LDKAKADIRTEQENAMKAMESRVAEIALDAASKIMGEKNSSQQDLSLYDQFIKEAGDS
                     NDGNKH"
     gene            5109..5612
                     /gene="atpH"
                     /locus_tag="ACZPD3_02790"
     CDS             5109..5612
                     /gene="atpH"
                     /locus_tag="ACZPD3_02790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR1414966.1"
                     /translation="MTETSINYAKALYELSVPEEAVLETEKIFRSTPQLKGALENPLV
                     SLKEKEHVIDRVFPQEMKNFLKVTCKYQKISSIYDILETYGDYSRKQKGILKAVLTYV
                     TKPEEAQKEKMEDFLRREFGAKEVILTLREDKSLIGGFILSAGDKEFDWSLRGRYNNL
                     RQKLTRR"
     gene            5629..7128
                     /gene="atpA"
                     /locus_tag="ACZPD3_02795"
     CDS             5629..7128
                     /gene="atpA"
                     /locus_tag="ACZPD3_02795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR1414967.1"
                     /translation="MNPDEIISILKEEIKNYDEISKDQEVGTVISVGDGIATVHGIDH
                     AMYGEVVTFENGLKGMVQDIRANSIGCILFGKDTGIKEGTKVARTQKQAGVPVGDAFI
                     GRIVNALGAPIDGKGEIKADGYRPVENPAPGIIDRKSVTVPLETGILSIDSMFPIGRG
                     QRELIIGDRQTGKTSVALDTILNQKGKDVICIYVAIGQKASTVAKIVSTLEKHGAMDY
                     TTVFSSTASDCAPLQYIVPYAGTALAEYFMYKGKDVLMVYDDLSKHAVAYRALSLLLE
                     RSPGREAYPGDVFYLHSRLLERSSRLNEKAGGGSITALPIIETQAGDLSAYIPTNVIS
                     ITDGQIFLESDLFNSGMRPAVNVGLSVSRVGGAAQAKAMKKAAGSVRIDLAQYREMEI
                     FTQFSSDLDEATTQQLKYGSGLMELLKQPLSSPLSLHEQVITLCAATHKAMMHVETKK
                     MKKYQRDMLDYFDSAYPEIGREIEEKRQLPDELAEKIVKVAEEFADKSR"
     gene            7137..>7623
                     /locus_tag="ACZPD3_02800"
     CDS             7137..>7623
                     /locus_tag="ACZPD3_02800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR1414968.1"
                     /translation="MGSTKEIQTRMKSIQDTMKITNAMYMISSSKMQKAKRILSDTEP
                     YYYNMQAAISRILRHMPDTEHPFFSIRHKIAEEDRKIGCIVVTGDKGMAGAYNHNIQK
                     MTEQFMAEHEHCKLYVLGMVGRQYFTKKHMDIAENFPYTVQKPTMHRARLISEEIVRA
                     FL"
BASE COUNT         2343 a         1390 c         1759 g         2131 t
ORIGIN      
        1 aggcgttggt attcaggtaa ttatcataac atatattata aggattttgg cagttatttt
       61 tacatatttc tgacattttt tacctccatg attatagcag accgaggcgg gatatctgta
      121 ttcatgtaat ttctcatttc tgaagatgcc ggactgctgc ataatgttat agtattagta
      181 tatgcggctg ttttattcat agacttacga gtgtttgcag caatattctc aagtaaaaaa
      241 agcagaccat tcgtatagaa tgaacaaaaa acagcattca aatttgtgaa aataataacg
      301 gaaaatatat tgcaatttct atggattgtg ttataatttc gataaataaa taatagcgaa
      361 gtaggatttt atgcgtcaag tgttcacagg ctggggagtc gccgtgaaac gaaaagcaga
      421 aagcgtgctg cttacgatat gatttccgca cccgttgcta catatgagag catctcataa
      481 tgtggaggga ttattattta tgtaactact ctatttttta aacacattat ggaggtgttt
      541 ttattatgca gaaaaaacaa tattggaacg taaaaacatt ggtgttcatg gctcttctga
      601 tcgccatgca tctggtactc acacgagttc tggtcattga tcttggtgcg taccgtattt
      661 ctgtgggaag tgtatgtaca atccttgcag gtctgtggct tggcccggta gctggtggtg
      721 tctgtggatt atgtgcggat attatcgggt gctttatgaa gggctatgct gtaaatccgt
      781 ttatcacagt tgcagcaatc ctctggggtg tgcttcctgc ccttgcaaaa ccattatttg
      841 caaacagaaa gaaaacagga aagacagttg gtatttgcgt atctatcgtg gtaactgcag
      901 ttctcagctc tttggttctt acaacagcag gacttgtgat catgcttgga tataatttct
      961 atgcgatcat gcccggaaga ctggtacagt ttgcgattat gatcccgatt tactgtgtat
     1021 taacatgtct gctgtatttc agcccgctga cagcgatggt tgtaggtaat acatcacctg
     1081 cagtgctgaa aaacaaaaca gtataattga gttactgttc acacaccact attccgcgag
     1141 gtgttttggc atgaatatgc caaaatcccg agacatacaa gccaaaattc cattgccgca
     1201 ggcaatctgg aatgaatttt ggctacgtta ctgtgaacgg agtgaacagt aacataattg
     1261 aggcagagat aaaatacaga ataagacttg aatattaaaa ttaaaatgat atacaacata
     1321 gttggcgcga actatgttaa atgaatcaac tgattccccg aaaaagaaaa gttaaaatct
     1381 ataaaaatta agaactatct gtaaaagcaa aaaatgaaaa cgctttacag atagttcttt
     1441 ttatttctca ggtgggaaat ttccgttttc atccggctgt acgcggaacc agtaagtata
     1501 aagctgctga aatccaagtg atctgtataa tgcccgggca ttgtcatttc cctctacaac
     1561 ctgaagatat gcattctgtg cacctttttt cattccttct ttcagaagtc ctgtgcagat
     1621 ctgacgggca tagccatgtt tgcggtattc ttctttgaca tggatggcgt aaataccgat
     1681 gtagtctctg tccaggatac caagccctgt tgcaatgatc tgcccgtcta tgcggatgca
     1741 ggcgcaaatg gtttctttga gaattgcctg atacatggat ggtacaactt ttctgtgaat
     1801 cggatttata gtatttttta gattgaacag actttcaatc cattcagacg gaatgttatt
     1861 gtagaatgtt acatcaggat atggagtatc cagatgtgcg gcgttcatgc tcattgccat
     1921 gacattggtg gtatgctgga tggcatatcc ccgattctcc agaacataat caaagtcagg
     1981 agagacaaga ggactgatct taaatacagc gggggttccc agacgcttat atacgctttc
     2041 acagtatggg attttttccc gccaggtgag ggtggaattg ccgaactgtt ctacgctgtt
     2101 ggttctgtgt gtataaaaat aagaaaatct caggatccag ccgtcgtata attccatctt
     2161 gtgagatggc caggcattca gagacatatc ttcaatctgt ttaattgtag tttccataat
     2221 ataaggaaga ctcctttcca taataagtcc gcagatatga gcgaggacag tgatttatta
     2281 tacaaagttc cgggaaaatg cgcaatagtt tatctgttca gggattgtta tcaatgtttt
     2341 gaaggagagg gaacagtttc tcagccagaa gcttatgccc atacaaagta agatgaatcc
     2401 cgtctgttgt tatggcatca aatcccgatt gagttgcttc cttattaaaa tagttatgca
     2461 gaggcagaaa tggaagtctg aatctgacag agagctgtcg gataatatca gacatggtat
     2521 ggacataggg aatccatgtc tcatattctt ccggatgtgg aaagataaag ggttccataa
     2581 ggattacctg cctggcatct gcagtgagca gattcaataa ttcattgtaa tgagtggcaa
     2641 actctctcat catctgttct ttctgagaat ccgtccggtc tgtgttcatt atcagaccga
     2701 tatcattgat gccgatcagc agagttataa caggagagcg atgcaaaaaa atacggtgtt
     2761 gacggatatt ttcaaggact cttgccacag tgaagccatc tatgccacag ttcctgaaat
     2821 gtatggcacc tgttgatttt gttaactgta cagcgcttga tgagcgtctg acggtatctg
     2881 cggatatgga gaaagaagga gtctgattac ggaacatttc tgagagtatc tgtacatacc
     2941 catttcccag aggaaaatct tcaaataaat gattacagtc cgtaatgctg tctcccagac
     3001 aaatgatttg cataaaaaac ctcctgtttg gtattaccaa tataaggatt ggtaagtgca
     3061 gagatataaa atttatataa aaaccatata aaataatatc atctgcttac agttcggtca
     3121 atgaaaattg cctttttttc gtgaaaatgc attaaaaaaa ttatgaaaat ctatttttca
     3181 atcaaaatgt ctggatgaaa tgcacaaaaa gaaaatctat ttttgccaga aatagtcaaa
     3241 ataattgttg actttttaac ggattttaat ataataatct caaaatcttg aatcgatgga
     3301 aagcttatga aatattttac agacataagt ttaggaatcc tggctgaggc atctgaggca
     3361 gacagcataa aaggccagaa tatcttccgg agatttaaat agaaaaagga gggaaaagaa
     3421 acgggtaatt tagcagacac gttaatggaa gaacttcaat gtaagacagt ttttaccata
     3481 ccggtgtttg gaggaatacc agttgcagag tcagtagcag tgacatgggt gatcatggca
     3541 gtgcttttaa ttttgtcatt ggtgctcgta agaaacctga gtgtggaaaa ccccggtaag
     3601 aaacagcttt tgctggaaac cggtgtcagt tttcttcata acttctttaa ggatatcctt
     3661 ggagaagagg gaaaaatgta tattccctat ctgatgacag ttgtcattta cattggaatt
     3721 gcaaacattt tcggtgtgtt tggatttgta ccgcctacaa aggatttaaa ctgtacgatc
     3781 ggtcttgcga tcacaagtat ttttctgatc gagtatgcag gattccataa gaaaggtctg
     3841 aaaggctttc ttaaaagctt cgcagagccg gcaccgatca tgctgccgat caatattctg
     3901 gaggtcgtta ttcgaccgac atcactttgt atgcgacttt ttggtaatgt gttaggaagc
     3961 tatgtggtta tgaaacttct tgagtttata tgcccggcag ttcttcctat tccattcagc
     4021 ctgtattttg atttctttga cggattcatt caggcgtatg tatttgtatt cttaacttct
     4081 ttatttatca aagaagctat tgaataaaaa agattactgt tcatacagga atatgacacc
     4141 tatataagac ctgagacgcg ggacataagg gataagacag cagttatgaa cagcacttaa
     4201 atgctgtccg gatatccggt gggtcataac ggtcataaac aaccgttggt acagacatct
     4261 gttgcacagg catcaatgat ctcatcggaa tatcgacagg atagcatatt gtgaagaaag
     4321 atttgtaata tttagtttat ttcagttaaa caacaggagg aaaaagttat gttagtagct
     4381 attggagcag gaatcgcagt tttaacaggt attggcgctg gtatcggtat tggtatggca
     4441 acatccaaag cagtagaagc aatcgcaaga cagccggaag cagaaagcaa gatcagcaaa
     4501 tcccttcttt taggatgtgc ccttgccgag gcaacagcta tttacggttt cgttatcggt
     4561 cttctgatcg ttatcatgct tggctaattg atggataaag aataactaag aaaggcaggt
     4621 gggacaggtg atcgagatta acatcaacct ggtctttaca attattaacc tgattgttct
     4681 ttatctttta atgaaaaagt tcttatttgg tccgatcctc aatgtgatgg agcagagaaa
     4741 aaacatgatc gaccagcagt tcgcaagtgc caaagataca gaagaacagg catatgaact
     4801 gaaaggcaaa tatgaagatg cactgaaatc tgcgaaagac gaatccatgc gtattgtaaa
     4861 ccaggcaaag gatgaggcaa aagtacaggc tgagcgtatt gtaaaagatg caaacacaca
     4921 ggctggtgca atgcttgaca aagcaaaggc agatatcagg acagagcagg aaaatgctat
     4981 gaaggctatg gaaagcagag tggcagagat cgcgctggat gcagcctcaa agatcatggg
     5041 agagaaaaac agcagtcagc aggatttgtc cctttatgac cagtttataa aagaggcagg
     5101 tgattccaat gacggaaaca agcattaatt atgcaaaagc attatatgag ctttctgtcc
     5161 cggaagaagc tgttttggag acagaaaaga ttttcaggag taccccgcag ttaaagggtg
     5221 ctcttgagaa tccgctggtt tctttaaaag aaaaagaaca tgtaattgac agggtattcc
     5281 cacaggagat gaaaaatttc ctgaaggtga cctgtaaata tcagaaaatc agcagcattt
     5341 atgatatcct ggaaacatat ggcgattaca gccgcaagca gaaaggtatc ctgaaagctg
     5401 ttctcacata tgtaacaaaa ccggaagaag cccagaaaga gaagatggaa gatttccttc
     5461 gcagggaatt tggagcaaag gaagtaatcc ttactctcag agaagacaaa tctttgatcg
     5521 gaggcttcat tctttctgcc ggagacaaag agttcgactg gagtctccgc ggacgttata
     5581 acaatttaag acagaaattg acacggaggt gaataaaagt tggctgcaat gaatccagat
     5641 gaaatcattt cgattctgaa agaagaaatt aagaattatg acgaaatcag caaggaccag
     5701 gaagtaggaa cagtcatctc cgtcggagat ggtatcgcca cggtacatgg tattgatcat
     5761 gctatgtatg gcgaggttgt aacctttgag aatggtctga aaggtatggt tcaggatatc
     5821 cgtgcaaaca gcatcggatg tatcctgttc ggtaaagata caggaatcaa agaaggaaca
     5881 aaggtagcaa gaacccagaa gcaggcaggt gtgcctgtag gtgacgcttt tatcggaagg
     5941 atcgtaaacg ctctgggagc gccgatcgat ggtaaaggtg agatcaaggc agacgggtat
     6001 cgtccggtag agaatccggc acctggaatt attgacagaa aatctgtaac agttcctctg
     6061 gaaacaggta ttctttctat tgactccatg ttccccatcg gccgtggcca gcgtgagctg
     6121 attatcggtg acagacagac aggtaagact tccgttgcac tggataccat tctgaaccag
     6181 aaaggcaaag atgtaatctg tatctatgtt gcaatcggac agaaagcttc cacagtagct
     6241 aagatcgtat ctactctgga gaaacatggt gctatggact atacaacagt attctcctcc
     6301 acagccagtg actgtgcacc gcttcagtat attgttcctt atgcaggtac tgcacttgct
     6361 gagtacttta tgtataaggg aaaagatgta ttaatggttt atgatgattt atccaagcat
     6421 gcagttgcat atcgtgcact gtcattgctt ctggaacgtt caccgggacg tgaggcttat
     6481 cctggagatg tattctatct ccattccaga ttactggagc gttccagccg tctgaatgaa
     6541 aaggccggcg gtggttctat cactgcactt cctatcattg agacacaggc aggtgacctt
     6601 tccgcatata ttcctacaaa cgttatttcc atcacagatg gtcagatttt cctggagagt
     6661 gacctgttta actccggtat gcgtccggcg gttaacgtag gtctttccgt atcccgagtt
     6721 ggtggtgctg cacaggcaaa agccatgaag aaagcagcag gaagtgtccg tatcgatctg
     6781 gctcagtatc gtgagatgga aatctttaca cagttcagtt ctgatctgga cgaggctaca
     6841 actcagcagt tgaaatacgg aagtggactt atggaacttc tgaaacagcc gctttcaagc
     6901 ccgttaagtc ttcatgagca ggtcatcact ctttgtgcgg caacacacaa ggcaatgatg
     6961 cacgtggaaa ccaagaagat gaagaagtat cagagagata tgctggacta ctttgacagt
     7021 gcttatccgg aaatcggaag agagatcgag gagaagagac agcttccgga tgaactggca
     7081 gaaaagatcg tgaaagtggc agaagaattt gcagataaga gcaggtaggt gttgatatgg
     7141 gcagtacgaa agagattcag actcgaatga aaagcattca ggacaccatg aagatcacca
     7201 atgccatgta tatgatctct tcatccaaga tgcagaaggc caaaagaatc ctgtccgata
     7261 cagagccgta ttactataat atgcaggcag caatttcccg aatcctgcgt catatgccgg
     7321 atacagagca tccttttttc agtatccgtc ataagattgc ggaggaagac cgtaagatcg
     7381 gctgtatcgt agttaccggt gataagggta tggcaggtgc ctataaccat aatatccaga
     7441 aaatgacaga acagtttatg gcagagcatg agcactgcaa gctttatgtg ctgggtatgg
     7501 taggccgtca gtatttcacg aagaaacata tggatatcgc agagaacttt ccatatacag
     7561 tacagaaacc gaccatgcac agagcgcgcc tgatctcaga ggagattgtc agagcgttcc
     7621 tgg
//
LOCUS       DBMDXG010000054         7612 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2527_length_7612_cov_1.787645, whole genome shotgun sequence.
ACCESSION   DBMDXG010000054 DBMDXG010000000
VERSION     DBMDXG010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_7612_cov_1.787645"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            160..1881
                     /locus_tag="ACZPD3_02805"
     CDS             160..1881
                     /locus_tag="ACZPD3_02805"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1414969.1"
                     /translation="MNTIKKFIHYYGPYKTIFFIDLICAAVISLIDLAYPQILRTMTK
                     TLFTQDQSRILRALPVIAGGLFLMYVIQCLCKYYVTCQGHMMGANMERDMRQELFDHY
                     QQLSFSYYSQNNSGQMMSKLVSDLFDISEFAHHGPENLFISLVKIIGAFVFLFFINKK
                     LAFPLIILVIVMFWFSFKQNARMQATFMENRRKIGDVNASLQDTLSGIRVVQSFANED
                     IEHNKFKKSNEAFLLSKRDNYRCMGSFMSSNLFFQGMMYLVTLVYGGYLIANGEMQTA
                     DLAMYALYIGIFISPIQILVELVEMMQKGLSGFRRFLDVMETESEITDAPDARELTDV
                     KGHVSYEHVSFHYSDDNTPVLSDISIDIPAGKSVALVGPSGGGKTTICSLLPRFYDVT
                     EGRITVDGKDIRSLTLKSLRNNIGTVQQDVYLFDGTIRDNIAYGKPGASDEEIIAAAK
                     RASIHDFIMELPQQYDTYVGERGTRLSGGQKQRISIARVFLKNPPILILDEATSALDN
                     ESERWIQRSLEELSQNRTTITIAHRLSTIRDADEIIVITEDGIAERGTHEELLDMNGV
                     YAAYYNM"
     gene            complement(2178..3410)
                     /locus_tag="ACZPD3_02810"
     CDS             complement(2178..3410)
                     /locus_tag="ACZPD3_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1414970.1"
                     /translation="MSRKNLIVGQSGGPTAVINSSLYGVVSEGMRHPEVIEHVYGMIN
                     GIEGFLNGTVLDFAEALPGEKLGGLKVTPGAYLGSCRYKLPESLEDPVYPRLFKKFEE
                     MNIGWFFYIGGNDSMDTVSKLSRYAAQTGSDIRILGEPKTIDNDLIHTDHTPGFGSAA
                     RYVASTVREITIDANVYEKKSVTIIEIMGRHAGWLTAASALARKYTGDNPLLIYLPET
                     AFDQEEFLKAVEKSFEKNCNVIVCVSEGIHDDKGTFICEYDNSVGTDTFGHKMLAGCG
                     KYLENLVRNRLGVKARSVELNVSQRCSASMMSATDQQEAIKAGEFGVQAALNGETGKM
                     ISFIRKETSDGTYIMECGLEDVNAICNEEKTVPSEWITEDGSDVTEAFINYARPLIQG
                     TVQIPCGEDGLPSFVYRK"
     gene            complement(3556..4947)
                     /locus_tag="ACZPD3_02815"
     CDS             complement(3556..4947)
                     /locus_tag="ACZPD3_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1414971.1"
                     /translation="MKRGSIFETDGIPRMSEALPLAMQHVVAMIVGCVTPAIIVSGAV
                     PGGLSREDQVILIQSALVIAALSTLLQLFPIGGKAKFAIGSGLPIIMGVSFAYVPSMQ
                     AIAESYGIAAIMGAEIVGGVVAVVMGLLVKKIRVFFPPLITGTVVFTIGLSLYPTAIN
                     YMAGGTSSPNYGSWQNWAIAFFTLIVVTALNHFGKGIWKLASILIGIIVGYLVSIPFG
                     MVDFSSIGEAGVCQLPSLMHFGVQFEPSSCVALGILFAINSIQAIGDYSATTIGAMDR
                     TPKDDELQRGIVGYGLSNVVGALLGGLPTATYSQNVGIVTTTKVINRWVLGLAAAILG
                     IAGLVPKFSAILTTIPQCVLGGATVSVFASIAMTGMKLVASAEMDYRNSSIVGLAAAL
                     GMGVSQATAALASFPTWVTTIFGKSPVVLATIIAVMLNVILPKTRDEQKEEAKKKIKI
                     EQKLEEDHREFGE"
     gene            complement(5108..6829)
                     /locus_tag="ACZPD3_02820"
     CDS             complement(5108..6829)
                     /locus_tag="ACZPD3_02820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR1414972.1"
                     /translation="MFGPDDFEVQIQQENPPGIHAEPSIPPAQDEEYGDFIVKHGQNM
                     GGKFLPVNELFGILYIPLNEIGPLEINSYTYASLPKCYTFMDADALNSSGITRLHNHP
                     YLKLQGEGTVIAVIDSGIDYLNPIFRNGDVSRIAYIWDQTIPGNEDEQVPYGKVFTGE
                     EINQALLSENPQEIVPSLDENGHGTAMAGLAAGNFVPTENFSGAAPKATIIVVKLKKA
                     KSYLRKFYQYPPQAPVFQEDDIMLGISFAVKMAQEMGMPVSVCLGLGTNQSAHVGDSE
                     LSRYVDYINEDSQVSVSVAAGNEGAAQHHYTAELDYVKNQDTVELRIADKEEGFSMEF
                     WGDPPDDYGISLQSPAGEKLYVSSSLGAGTQELSFIFVETKVLVNYVKMERMTGKQLI
                     YFRFFHPAAGIWKVNVSKKGISGSRFHMWLPVQGLISPDTYFLESTPYITVTAPGDST
                     RGITATAYQYLDNSLYFQAGRGFTPNNQVTPDLAAPGVDLLIPLPGGAFGKASGSSLS
                     SAVVAGAAALVQEWAIVRGNIPYASGNTVKFYLQKGAVREEQMEYPNPGWGYGRLDLY
                     RTFEIIN"
     gene            complement(6890..7561)
                     /locus_tag="ACZPD3_02825"
     CDS             complement(6890..7561)
                     /locus_tag="ACZPD3_02825"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1414973.1"
                     /translation="MKLACEHIFYLVVWCAIGGFTSAKILFWITNWREFLQNPRQIIG
                     SDGFVVYGGIIGGILVGWLYCRIKKLKFLEYFDLMMPSIALAQGFGRIGCFLAGCCYG
                     KETSGPLAVTFTNSDFAPNNVALIPTQIYSGFLDFAHFLLLLYVAKHKKADGQVAACY
                     LIFYSIGRFVIEFFRGDIERGSVGVLSTSQFISIFTTVAGIILLLTVVKKQKQEANIS
                     LNSKG"
BASE COUNT         2080 a         1806 c         1510 g         2216 t
ORIGIN      
        1 atcagatcat cttcctctcc tgtgaaatta ccataaccac aatataacac aaaaaagctt
       61 cattttaaag tcctgttcct gcccctctat tgactttcct ttctgttatg ttactataaa
      121 atcacatata ttctatcaca gatgaaagga ttttatctta tgaatactat caaaaaattc
      181 atccactact atggtccgta taagacgatc ttttttattg atctgatatg tgctgccgtg
      241 atcagtctga tagatctggc atatcctcag atcctgcgga ctatgacaaa aacactgttc
      301 actcaggatc agtcaaggat tcttcgtgca ctgcctgtga tcgcaggagg actctttctt
      361 atgtatgtga tccaatgtct ctgcaaatat tatgtaacct gccagggaca catgatggga
      421 gcaaatatgg aacgggatat gcggcaggaa ttatttgatc attaccagca gctttcattt
      481 tcctattaca gtcagaataa ttccggtcag atgatgagca agctggtcag tgatcttttt
      541 gatatttccg agtttgcaca ccatggtcct gaaaaccttt ttatctctct ggtaaagatc
      601 atcggtgctt ttgtctttct tttctttatc aataagaaac tggcatttcc actgatcatc
      661 cttgttattg ttatgttttg gttttccttt aagcagaatg cccgaatgca ggcaactttt
      721 atggaaaaca gacgtaagat cggggatgtc aatgcaagtc tccaggacac actttccggt
      781 atccgtgtgg tacagtcctt tgcaaatgaa gatattgaac ataacaaatt caaaaaaagc
      841 aatgaagctt ttctgctttc aaagcgtgat aattatcgct gtatgggaag ctttatgagc
      901 tctaatctgt tctttcaggg aatgatgtat ctggtcactc tggtgtatgg cggttatctt
      961 attgccaacg gagaaatgca gactgcagat cttgccatgt atgcacttta tatcggaatc
     1021 tttatcagtc cgatccagat tctggtcgag ctggtagaaa tgatgcaaaa gggtctttca
     1081 ggctttcgcc gtttccttga tgttatggaa acagagtcag agatcacaga cgctccagat
     1141 gccagagaac ttactgatgt gaagggacat gtaagctatg agcatgtatc ttttcattac
     1201 agtgacgata atacccctgt tctcagtgat atctctattg atatccctgc cggaaagtct
     1261 gtggcacttg tgggtccgtc aggtggcggg aaaactacta tctgttctct tctgccacgc
     1321 ttttatgatg taacagaggg acggatcact gttgatggaa aagatatccg cagcctgact
     1381 ctgaagagtc tcagaaataa tatcggaact gtacagcagg atgtctacct gtttgacggt
     1441 acgatccggg ataatattgc atatggaaaa ccgggagctt ctgatgaaga gattattgca
     1501 gctgcaaagc gggcaagcat tcatgatttt attatggaac ttccacagca gtatgatacc
     1561 tatgtcggag aacgcggaac aagactttcg ggcggtcaga aacagaggat ttccattgca
     1621 cgtgtgttcc tgaagaatcc gccgatcctg attcttgatg aagctacgag cgctctggat
     1681 aatgagagtg aacgatggat tcagagaagt cttgaagagc tttcacagaa ccgtactact
     1741 attaccattg cgcacagact ttctacgatc agggatgcgg atgagattat tgtgatcact
     1801 gaggatggga ttgctgagag ggggacacat gaagagctgt tggatatgaa tggagtgtat
     1861 gcggcttatt ataatatgtg attttgttta tagtatccga taataaactg cgaaacagtt
     1921 atctttatat ttgcaatcta ctgatatata ttataacagc gcaggaaaat taattgtcta
     1981 gcgtgcattt gcaggatact tagatattct tagacgataa aaatatgcgt tatgagccca
     2041 cagggtcact gtctgagcga cagcgagttt gaccctgcgg gctcatgctt ttagcagatt
     2101 tttatcgcct tagaatatct tagtgtccga aaccgcacgc cagacaatta attttcctgc
     2161 gcgtccgtca atctatacta ttttcggtat acgaatgacg ggaggccgtc ttcgccgcag
     2221 gggatctgta ctgttccctg gatcaggggg cgggcgtagt ttatgaaagc ttcagtgacg
     2281 tcagatccgt cttctgtgat ccactctgat ggaactgttt tttcttcgtt gcagatggca
     2341 ttgacgtctt caagaccaca ttccatgata taggttccgt cagaggtttc tttgcggata
     2401 aatgagatca tttttcctgt ttcgccgttg agggctgcct gtacgccgaa ttctccggct
     2461 ttgatggctt cctgctggtc agtggcggac atcatggatg ctgagcaacg ctggctgacg
     2521 ttgagttcta cagaacgggc ttttacaccg aggcggtttc tgacaaggtt ttcgagatat
     2581 ttaccacagc ctgccagcat tttgtgtcca aaggtatcag tgcctacgga attgtcgtat
     2641 tcacagataa atgtgccctt gtcgtcatgg attccttctg atacgcagac aatgacattg
     2701 cagtttttct caaaggattt ctctactgct ttaagaaatt cttcctgatc aaaggctgtt
     2761 tccggaaggt agatgagaag agggttgtct cctgtatatt tgcgggcaag tgcagatgca
     2821 gcagtgagcc atcctgcgtg gcggcccata atttctataa tcgttacgga tttcttttca
     2881 taaacatttg catctatggt aatctcgcgg actgtagaag ctacatatct ggctgcactt
     2941 ccaaatcccg gagtatggtc tgtgtggatc agatcattgt cgattgtttt tggttctccg
     3001 aggatacgga tatcactgcc tgtctgcgca gcataacggg aaagtttgct caccgtatcc
     3061 atggaatcat ttccaccgat atagaaaaac cagccaatat tcatttcttc aaatttttta
     3121 aaaagtcttg gataaaccgg atcttccagt gattccggca atttatagcg gcaggaacca
     3181 agataagcac ctggtgtgac tttcagaccg ccaagtttct ctcccggaag tgcttcggca
     3241 aagtcaagaa ctgttccatt taaaaatcct tcaattccgt ttatcattcc gtaaacatgc
     3301 tcaataactt ccggatgacg cattccctcg gatacaactc cgtaaaggct gctgttaatt
     3361 actgcggtag gaccgccgga ctgaccgacg atcagattct ttctggacat aagaaacctc
     3421 ctgtatcgtt tgatttaatg tgattgcttt tcattatatc atacaactgc cttccatagc
     3481 aattcaataa aaaggtgtgt tcccacatcc ccctgtgaaa gcacacctta tatttcccat
     3541 attcagtttc tatttttatt ccccgaattc cctgtggtct tcttccagtt tctgttctat
     3601 tttaatcttt ttttttgcct cttctttctg ctcatcccgt gttttcggaa ggatcacatt
     3661 aagcattact gcaataatag ttgcaaggac aaccggtgat ttaccgaaaa ttgttgttac
     3721 ccatgtggga aagcttgcaa gtgcagctgt tgcctgagat acacccattc ccagtgcagc
     3781 tgcaagtcct acgatagatg aattacggta atccatctca gcagaagcaa ccagcttcat
     3841 acctgtcatt gcgatcgatg caaatacaga cacagttgca ccacccagta cacactgagg
     3901 aatcgttgtc aggatagctg agaatttcgg aaccagacct gctataccga gaattgcagc
     3961 tgcaagtccc agtacccaac ggttgataac ctttgtagtt gtaacaatac caacgttctg
     4021 gctgtatgtt gctgtgggaa gtccgccgag caatgcgcct acaacgttag aaagaccata
     4081 accaacgatt ccacgctgca gttcatcatc cttaggtgtt ctgtccatag ctccgatcgt
     4141 agtcgctgaa taatcaccga ttgcctgaat agagttgata gcaaataaaa ttcccagtgc
     4201 aacacaagaa gacggctcaa actgtactcc aaaatgcatc agagatggaa gctgacagac
     4261 acctgcttcc cctatgcttg agaaatctac cattccaaac ggaatagaaa ccagatatcc
     4321 cactataata ccgatcagaa tagatgccag tttccagatt ccctttccaa aatgattcag
     4381 agctgtaaca acgatcagcg tgaaaaatgc aattgcccag ttctgccagg atccgtaatt
     4441 cggactgctg gtaccgcctg ccatataatt aatggcagtc ggatacaggg aaagaccgat
     4501 ggtaaatacg actgttcctg tgattagcgg cgggaaaaat acccgaatct tcttgaccag
     4561 caaacccata acaactgcta cgacaccacc aacaatctcc gcacccatga ttgctgctat
     4621 tccatacgat tccgctatcg cctgcatact tggcacgtaa gcaaagctga cacccatgat
     4681 aataggcagg cctgagccaa ttgcaaattt cgcctttccg ccaatcggaa acagctgaag
     4741 caaagttgac aatgctgcaa tgaccagtgc cgactggatc aggataacct ggtcttctct
     4801 ggagagtccg cctggaactg ctccggaaac gatgatcgcc ggtgtgacac agccaacgat
     4861 catagctacg acatgctgca tggcaagagg taaagcctca ctcatgcggg ggattccgtc
     4921 agtttcaaaa atactgcctc tcttcataat tgcctcccaa tttcttttgt acgattgtaa
     4981 cattttttgt ccaaaaaaac aattagacag cctaattatt tttggtctaa aactatacaa
     5041 tatttactct taaaatttgg ttattatttt atcttttata tgtattttcc agtatttcat
     5101 aaacctttca gtttataatc tcaaatgtcc gatacagatc cagtcttcca tatccccatc
     5161 ccggattggg gtattccatc tgttcttctc tcactgcccc tttctgcaga taaaacttca
     5221 ctgtatttcc cgatgcataa ggaatatttc ctctcacgat cgcccattcc tgcaccaatg
     5281 ctgctgctcc tgcaacaaca gccgaagaaa gactgctccc cgaagccttt ccaaacgccc
     5341 ctcccggcaa tggaatgagc agatctactc ccggtgctgc cagatcaggt gtcacctgat
     5401 tatttggagt aaaccctctc ccagcctgaa aatacagact gttatccaga tactgataag
     5461 ctgttgccgt aatacctctg gttgaatccc caggtgcagt aacagttata taaggtgtag
     5521 actccagaaa ataagtatcc ggcgaaatca gcccttgcac cggaagccac atgtgaaacc
     5581 tgcttcccga tattcctttt ttactgacat ttaccttcca gatacctgct gccggatgaa
     5641 agaagcgaaa ataaatcagc tgtttcccgg tcatcctctc catctttacg taattgacca
     5701 gcactttcgt ctccacaaaa atgaaactta attcctgagt acctgcacca agagaactgc
     5761 tcacatacag cttttctcct gccggtgact gtaacgatat cccatagtca tccggcggat
     5821 ctccccaaaa ttccatggaa aatccttctt ctttatccgc aatacgaagt tcaacagtat
     5881 cctgattttt cacatagtcc aactctgctg tataatgatg ctgtgctgca ccctcatttc
     5941 ctgctgccac agatactgat acctgagaat cttcattaat ataatctaca tatcgactca
     6001 gctcactgtc accaacatgt gcgctctgat tggtgccaag tcccagacaa acggaaaccg
     6061 gcattcccat ctcttgtgcc atcttcactg caaaagaaat ccccagcata atgtcgtctt
     6121 cctgaaatac cggtgcctgt ggcggatact ggtaaaattt tctcagatag gattttgctt
     6181 tctttaattt caccacaatg atcgttgctt tcggagctgc tcctgagaaa ttttctgtgg
     6241 gtacaaaatt gcctgctgcc agccctgcca tagctgtacc atgtccgttc tcatccaatg
     6301 agggtacaat ctcctgtgga ttctccgaca gcaatgcctg attaatttcc tctccggtaa
     6361 aaacctttcc ataaggaacc tgttcatcct catttcccgg aattgtctga tcccagatat
     6421 atgcaatacg gcttacatct ccgtttcgaa agattggatt cagataatca atcccggaat
     6481 caatcactgc gatcactgtg ccctctccct gaagtttcag atagggatga ttatgtaacc
     6541 gcgtaatccc ggatgaattt aatgcgtccg catccataaa tgtataacac ttcggcaaag
     6601 atgcatacgt ataggaatta atttccagcg gaccaatctc attcagcgga atatatagaa
     6661 ttccaaacaa ttcattcaca ggaagaaatt ttccacccat attctgtcca tgttttacaa
     6721 taaaatcccc gtactcttca tcctgtgccg gcggtattga cggctcagca tgaatgcccg
     6781 gggggttttc ctgctgtatc tgcacttcaa aatcgtcagg tccgaacatt actgtcctcc
     6841 ctggattttt tatacatgat atgcacaata tatgctcttt atgacaattc taacctttac
     6901 tgttcagact gatattcgct tcctgctttt gtttcttcac aacagtcagt aataatataa
     6961 ttccagccac agttgtaaaa atactgataa actgcgaagt cgacaatact cctacgcttc
     7021 ctctttcaat atctcctctg aaaaattcga tcacaaatct tcctatactg tagaaaataa
     7081 gatagcaggc agccacctgt ccatctgctt tcttatgctt tgccacataa agcagcaaca
     7141 gaaaatgggc aaaatccaaa aaaccggaat aaatctgtgt cgggatcaga gcaacattgt
     7201 tgggtgcaaa atctgaattt gtaaacgtaa ccgccagagg ccctgaagtt tctttcccat
     7261 aacagcagcc tgccagaaag catccaatcc tgccaaatcc ctgtgccagt gcaatggatg
     7321 gcatcatcag atcaaaatac tccagaaatt tcaacttctt tatcctgcag tacagccagc
     7381 ccaccagaat tcctccgatg attcctccat ataccacaaa tccatctgat cctatgatct
     7441 gccttggatt ctgcagaaat tccctccagt tggtgatcca gaacaaaatc ttcgcagatg
     7501 taaatccacc aatggcgcac cacactacca gataaaaaat atgttcacat gcaagtttca
     7561 gtttacgcgt tctttgggat cagccaaaca ccagccatct ttacagcgcc gg
//
LOCUS       DBMDXG010000055         7607 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2529_length_7607_cov_2.048095, whole genome shotgun sequence.
ACCESSION   DBMDXG010000055 DBMDXG010000000
VERSION     DBMDXG010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_7607_cov_2.048095"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="ACZPD3_02830"
     CDS             complement(<1..560)
                     /locus_tag="ACZPD3_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1414974.1"
                     /translation="MEKKKKGVMQSIPGWAWVLISLAAAILLWYALSINPKTARSFPF
                     IPAILEAAKRMIDNGALGQDFCSSMISILAGFALGFITAVPIAFLMAWYLPVRGVLEP
                     WIQFIRNIPPLAYVPLVVITAGVGRKPQIIVIWIATFLIMTVTIYQGVRNIDNTLVKA
                     ARVLGAKDRDIFVKVIFPATTPFILTA"
     gene            complement(615..1781)
                     /locus_tag="ACZPD3_02835"
     CDS             complement(615..1781)
                     /locus_tag="ACZPD3_02835"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR1414975.1"
                     /translation="MSQNVLVVHGGGPTAVINASLYGVIEEAKRNPEVGKVYGAIGGT
                     GAIFSETFIDLTSFDDEKLKLLLHTPASAIGSSRYPLYEKDYEKMVDIFKKHDIKYVL
                     LNGGNGTMDACGHIYEVCKDQDIKVVGIPKTIDNDIAMTDHSPGYGSAARFIAASTQE
                     VGEDVKSLPIHVCIMEAMGRNAGWITAASALARRKQGDAPHLIYLPERNFNEDEFLED
                     VSRLQKKQGGVVVLVSEGLHDANGESIVPPIFKSERAVYYGDVSSHLANLVVKKLGVK
                     ARSEKPGLCGRASVALQSPVDREEARQAGTVALRSAVEGNSGVMVGFERVPGTVYQMK
                     TKLVPIKEVMLYERKMPDNFINERGNDVTQEFVDWCRPLIGDPLPEFLNFKDYL"
     gene            complement(2177..2746)
                     /locus_tag="ACZPD3_02840"
     CDS             complement(2177..2746)
                     /locus_tag="ACZPD3_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1414976.1"
                     /translation="MIYIQLFLSFLQVGALSFGGGYAAMPLIQEQVVNLHSWLSMSEF
                     TNLITIAEMTPGPIAVNSATFVGMQIAGILGAIIATLGCILPSCIIVTLLAYVYMKYR
                     NMSLLQGTLASLRPAVVSMIAKAGVTILISAFFIDGTVNLIRQNVCVEMIIFFVIALV
                     LLRKFKINPILAMVLCGVANVVLSAVKKA"
     gene            complement(2743..3318)
                     /locus_tag="ACZPD3_02845"
     CDS             complement(2743..3318)
                     /locus_tag="ACZPD3_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR1414977.1"
                     /translation="MEEKTKSKGYVLRKLFFACLYLSTFTFGGGYVIVTLLKEKFVDH
                     YHWIEEDEMLDLVAIAQSSPGAIAVNGAIIVGYKLAGIPGMLVSVIGAIIPPMVILSV
                     ISVFYDAFCSNYYIAALLKGMTAGVGAVIMSVVYDMGKNVVKSKDWVNVVIMIISFCL
                     SYFLNVNIIYIILLVAVFGMVRTIVKGGREK"
     gene            3574..4614
                     /locus_tag="ACZPD3_02850"
     CDS             3574..4614
                     /locus_tag="ACZPD3_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1414978.1"
                     /translation="MTLREIAAEAGVSISTVSRVINQSTNSPVSREVQDRIWEIAKRT
                     NYIPRHSKKNTVEPDSSSTEPFRSIACLFARVPDGASDYFFSTLAKNIEQEAFRHNYI
                     LKYTFSAFDIDDPFTLQLIASNDIDGIAVLGRCDKELLKFLKKHFQYVVYSGLNNLDA
                     KYDQIICDGTQISYDVVTRLIEQGHRKIAYIGETQNENRYVGYCSALTDADISVSPKY
                     VSNIVHSTEEGYKGVCHLLKSGCDATAFFCADDITAIGAMKAIKEYGLRIPEDVSVAS
                     IDDIDTAQYLIPSLTTAHIPLDEMGKMAAKILIDRIEGGHEKHLKILFPYDIIERKST
                     AAFRKIHGNPQK"
     gene            complement(4903..7527)
                     /locus_tag="ACZPD3_02855"
     CDS             complement(4903..7527)
                     /locus_tag="ACZPD3_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1414979.1"
                     /translation="MLLIGYEFIKAIQYAAMNRLLQRKVYLDDATGLPNKNKCEELLS
                     EEEPDADTGVCSFDLNNLRRINDSRGHEAGDAYIRRFAICLRASMPAEQFVGRAGGDE
                     FLAVTHGLDREQMTQCLEKVRRDMREESKVYPDTPLSYAAGFALAGDFPRSTMRELFN
                     CADKNMYINKNHVKREEAAAEKHQGYQLLKLLNQHGSNFSDCLYCDARMDTYRAIRSS
                     ENFFLASDGAYSGAVEQIIEEQVEKSSQADIREGLQISELQKKMHTKKDVLEYEYNIG
                     KQGAYNRLTLIPVDWDEDKKLHHFLLAFETIRKTSEGQTGAKEQLQLYYEQLKQSILE
                     NDSYVDALLELSDVIYTVNLTKDALERRIVLNGKEQKSRELFMDYPLPCSYRDYCWEY
                     EKKITQETIAGYCMTDNCEKLRKRFENGETNMSVEYCAREDDGSIRWVQKTVLMTRMV
                     VFDTEILAEVPMIYAIILLQDTTQRHERDEQEQARLQAAFNEMRAESRAKTNFLSRMS
                     HDIRTPLNGIIGLLKIDETHFEDKELIQENHKKMKIAADYLLSLINDVLQMSKIEEGH
                     IVLTHEYICLKDLVYEIESIITHKAADEDIQWIYEKNKENIPYPYVYGSSLHLRQIFL
                     NIYGNCIKYNRPGGKITTVMEAADVHDGICTYRWTISDTGIGMSLEFLSHIFDPFSQE
                     KTDARSVYQGTGLGMAITKGLIEQMNGSIEVTSQVGVGSVFVITIPFEIAQEQKKDEE
                     IAEKYDIRGLHLLAAEDNELNAEIIEMLLTDDGAKVTVAKNGRQAVEHFENNPPGTFD
                     AILMDVMMPVMDGIAATKAIRAMDRADAKTIPIIAMTANAFEEDAKRCLAAGMTAHLA
                     KPFQIEDVEKTIVECCGK"
BASE COUNT         2130 a         1784 c         1270 g         2423 t
ORIGIN      
        1 gctgttaaaa taaacggggt tgttgccggg aaaataactt ttacaaatat atctctgtct
       61 tttgcgccaa gaactctggc tgcttttact aaagtattat caatgtttct tacaccctga
      121 tagattgtaa ctgtcatgat caggaatgtt gcgatccaga taacgatgat ctgtggtttt
      181 ctaccaacac cagctgtaat aactaccaac ggtacatatg ccagaggtgg gatgttacga
      241 ataaactgaa tccatggttc cagaactcct ctgaccggca ggtaccatgc cataagaaat
      301 gcaatcggta ctgcggttat aaagccgagt gcaaatccgg ccaggattga aatcattgaa
      361 ctacaaaaat cctgtcccag tgccccatta tcaatcattc gtttggctgc ctcaagaata
      421 gccggaataa atgggaagct tcgtgctgtt tttggattaa tggataatgc ataccataac
      481 aggattgctg ctgcaaggga aatcagtacc catgcccatc ccgggataga ctgcattact
      541 ccttttttct ttttttccat agttactctc ctttcaatgg aacattttca tttttgtttt
      601 gtatattttt cgttttaaag ataatccttg aaattcagga attccggaag tggatctccg
      661 atcagcgggc gacaccagtc aacaaattcc tgtgttacat cgttacctct ttcatttata
      721 aaattgtcag gcatcttccg ttcataaagc ataacttctt tgataggaac aagctttgtt
      781 ttcatctgat acaccgttcc cgggacccgt tcaaaaccta ccatcactcc gctgtttcct
      841 tcaacagcac tcctcagtgc aactgtgcca gcctgtcttg cttcttctct gtccaccggt
      901 gactgaagtg ctacagaagc tcttccgcaa agtccgggtt tttcgcttct tgcttttacg
      961 ccaagtttct taactaccag attcgcaaga tgtgaactta catctccata ataaacagct
     1021 ctttctgatt taaagatcgg aggaacaata gattctccat ttgcatcatg aagtccctca
     1081 cttaccagaa ccactactcc accctgtttt ttctgaagcc ttgatacatc ttcaagaaac
     1141 tcatcttcat tgaagtttcg ttccggcaga taaatcaggt gaggtgcatc tccctgtttc
     1201 cttctggcca atgccgatgc agctgtgatc catccggcat ttcttcccat ggcttccata
     1261 atacatacat gaatgggaag agattttaca tcttcaccaa cttcctgggt agaagctgca
     1321 ataaatcttg ccgcacttcc atatcctggt gagtgatctg tcattgcgat atcattgtca
     1381 atcgtttttg gaattccaac aactttgata tcctgatcct tacagacctc ataaatatgt
     1441 ccgcatgcat ccatagttcc gtttccgcca ttcagaagaa catatttaat atcatgtttt
     1501 ttaaagatat ccaccatttt ttcataatct ttttcatata gaggatatct agaagatcca
     1561 attgccgaag caggtgtatg caaaagaagt ttcaattttt catcatcaaa cgaagtcaaa
     1621 tctatgaaag tttctgaaaa aattgctccg gttccaccaa tcgctccgta aacttttcca
     1681 acctccggat ttcgttttgc ttcttcgatc acaccgtaaa gggatgcatt gatgactgct
     1741 gtaggcccac ctccatgtac cactaataca ttttgtgaca tattccgctt tcctttctat
     1801 tgtttattgt gcatcttaca ataaactttt tatttttctt gaatatgttt tattcaattt
     1861 cactttgtac tcttatctta acatattttt acaaaaacaa aagcacaatt agagaaaacg
     1921 ttgtctggat attttgtata atttgccatg ttgttttttg tctatttttt attattattt
     1981 tatattattc tttatttttt catccgtttt cacttttttt caaatatccg tccaaatttt
     2041 acaggtttat agaaaacaaa gagatcaaaa agacgttcat ctttttgatc tctttgtttt
     2101 ttattgttat atgtctgatt aatttgacta tttgcagtcc gtcattacac aaacgtttgc
     2161 tttatcatat agtgatttac gcttttttta cagcgctcaa aaccacattt gccacaccac
     2221 acaaaaccat tgccaatata ggatttattt taaatttacg aagcagcacc aacgcgatca
     2281 caaaaaatat gatcatttcc acacatacat tctggcgtat aagatttaca gtaccatcaa
     2341 tgaaaaatgc agaaattaaa atcgttaccc ctgctttggc gatcatcgat acaactgccg
     2401 gtctgagaga tgccagtgtt ccctgaagta aagacatatt ccgatacttc atataaacat
     2461 atgccagcaa ggttacaata atacaggagg gaagaataca tcccaatgta gcaattattg
     2521 cacctaagat tcccgctatc tgcatcccta caaaagtagc agagtttact gcaatcggtc
     2581 ccggcgtcat ttcagcgatc gttatcagat tcgtaaattc gctcattgaa agccagctgt
     2641 gcagattcac cacctgttcc tgtatcagcg gcattgcggc atatcctcct ccaaaactca
     2701 atgccccgac ctggaggaaa cttaaaaaaa gctgaatata gatcattttt ccctgcctcc
     2761 ttttactatt gtccgtacca ttccaaatac agctaccaaa agtattatat agataatgtt
     2821 aacattcagg aaatagctga gacaaaaact gatgatcatt atcaccacat tcacccagtc
     2881 ctttgacttt acaacatttt tccccatatc atacactacg gacataataa ctgccccaac
     2941 tcccgcagtc attcctttta ataatgccgc aatataataa ttggaacaaa acgcatcata
     3001 aaatacagaa ataacagata agatcaccat cggaggaatg atcgctccaa tgacagatac
     3061 cagcattccc ggtatgccgg caagcttata ccccacaata atcgctccgt ttactgcaat
     3121 cgctccaggt gaagactggg cgatcgcaac cagatccagc atttcatctt cttcgatcca
     3181 gtgataatga tccacaaatt tttctttcag caaagtaaca attacataac cgccgccgaa
     3241 agtaaatgtg ctgagataca gacatgcaaa aaataatttt cgcagtacat atcctttact
     3301 ttttgttttt tcttccattt tatttccccc attttttaat gcattctata gattctatat
     3361 tttatcttat catgtactaa aaaaaattca cttcatttat cagcaaacgt ttgctttata
     3421 ttttcataca tttcttttat tgcatctgta tttttgtctt tgaatcaact atacttgtgt
     3481 taacaatata gctgtaaatt catacagcaa tctgacatcc tggttctgac ataagtatca
     3541 taaaacactg caccccaaga aaggaggtct cctatgacgc tgagagaaat tgcagcagaa
     3601 gcaggcgtat cgatttcaac agtctcacgt gtaatcaatc aaagtactaa tagccccgtg
     3661 agcagggaag ttcaagaccg tatatgggaa atagccaaac gtaccaatta tattccaaga
     3721 cattcaaaaa aaaatactgt agaacctgat tcatcttcca cagaaccatt tcgttctatt
     3781 gcctgtctct ttgcacgggt tccggatgga gcatctgatt atttcttctc tacattggcc
     3841 aaaaatattg aacaggaagc attccgtcat aactatattt taaagtacac attttctgct
     3901 tttgatatcg atgatccttt tactctacag ttaattgcca gcaatgatat tgacggcatt
     3961 gccgttttgg gtcgttgtga taaagagctt ttgaaatttc tgaaaaaaca tttccagtat
     4021 gttgtttatt ccggtctgaa taatctggat gcaaaatatg atcagattat ctgtgatgga
     4081 acacaaatca gttatgatgt cgtaacccgc cttatagaac agggtcatag aaaaatcgct
     4141 tacatcggcg aaacacagaa tgaaaaccgg tatgttggat actgttctgc attgacagat
     4201 gcagatattt ctgtcagtcc caaatacgtc tctaacattg ttcattccac agaagaggga
     4261 tacaaaggtg tctgccatct tcttaaaagc ggctgtgatg caactgcttt cttctgtgct
     4321 gatgatatta cggcaatcgg tgcaatgaaa gctatcaagg aatacggtct tcgcattccc
     4381 gaagatgtat ccgttgccag cattgatgat atcgatacag cacaatatct gattccctca
     4441 ttaacaacag cacacattcc tcttgatgaa atgggaaaaa tggcagccaa aatactgatt
     4501 gaccgtattg agggcggtca tgaaaagcat ttaaaaatat tatttcctta tgatatcatc
     4561 gaacgtaaga gtactgctgc cttccgtaaa atccatggca accctcaaaa ataatttgct
     4621 tatagcaatc ctcgtaaata atgtatctta atccaattaa ttctgcagtg gatgaacgca
     4681 aaaaagaagg tgcaaaccac atttctatga tttgcacctt ctcttattct atatcagtag
     4741 ttcaatctca gataaaactc tttcagcaat cagtcttatc agccttcctg tgatgactgt
     4801 cagttctgtg actcacgcct ttctctactc atattttcgt catccagact tttaactcta
     4861 tttatgataa cttactcata acaacagacc ttacagatgg catcactttc cacaacactc
     4921 cacaatggtt ttctccacat cttctatctg gaaaggtttt gccaggtgtg cagtcatgcc
     4981 ggctgcgaga catcttttgg catcttcctc aaatgcattt gcagtcattg caatgatcgg
     5041 aattgttttt gcatctgccc tgtccatggc acgtatggct tttgttgctg caataccatc
     5101 cataacaggc atcatgacat ccatcaatat ggcatcaaag gttcccggag gattattttc
     5161 gaagtgctcc actgcctgtc tgccgttttt tgcaactgta acctttgcac cgtcatctgt
     5221 cagcagcatt tctatgattt cagcattaag ctcattatcc tcggctgcca gcagatgcaa
     5281 tccacgaata tcatatttct cagcaatttc ctcatccttt ttctgttcct gggcaatctc
     5341 aaagggaatg gtaatgacaa acacagatcc aactcccacc tgacttgtta cttcaatact
     5401 tccattcatc tgctcaatca agcctttagt gatcgccatt cccagaccgg ttccctgata
     5461 aacactacgg gcatctgttt tttcctgtga gaacggatca aaaatatgac tcaggaactc
     5521 cagactcatc cctatacctg tatcagaaat cgtccatctg tatgtacaga ttccatcatg
     5581 tacatctgca gcctccatta ccgtagtgat ctttcctccc ggccggttat acttaataca
     5641 gtttccatag atgttcagaa aaatctggcg aagatgcagt gagcttccat agacataagg
     5701 ataagggata ttctccttat ttttctcata aatccactga atatcctcat ctgctgcctt
     5761 gtgagtgata atactctcaa tctcatagac cagatctttc aggcagatat attcatgagt
     5821 cagaacaata tgaccttcct ctattttact catctgcaga acatcattga taagggaaag
     5881 caaataatcc gctgctatct tcatcttttt atgattttcc tgtatcagtt ccttatcctc
     5941 aaaatgagtc tcgtcaatct ttaacagacc aataatacca ttcagaggtg tcctgatatc
     6001 atggctcata cggctgagga aattcgtttt tgccctgctt tcagcacgca tctcattgaa
     6061 ggcggcctga aggcgtgcct gttcctgctc atcccgttca tgtctctgtg tggtatcctg
     6121 cagcagaata atcgcataga tcataggcac ctcagcaaga atttctgtat caaacaccac
     6181 catcctggtc ataagaactg ttttctgtac ccagcggata cttccatcat cttcgcgggc
     6241 acaatattcc acagacatat ttgtttctcc gttctcaaac ctcttacgaa gtttttcaca
     6301 gttatctgtc atgcagtaac cggcaattgt ttcctgggtg atttttttct catattccca
     6361 gcaataatcc cgataagaac atggaagcgg gtagtccata aaaagttccc ggctcttctg
     6421 ttcttttcca ttcagtacga tccttctttc cagagcatcc tttgtcagat ttactgtata
     6481 gatcacatct gacaattcca gaagcgcatc cacatagctg tcattttcca ggatagactg
     6541 tttcagctgt tcataataga gctgcagctg ctctttcgca cctgtctgac cttcagaagt
     6601 ctttcggatg gtttcaaatg ccagaagaaa atgatgcagc tttttgtcct catcccagtc
     6661 caccggaatc agtgtcaagc ggttatatgc cccctgtttt ccgatattat actcatattc
     6721 cagaacatct ttcttcgtat gcatcttctt ttgaagttcc gaaatctgca gtccttcgcg
     6781 gatatctgcc tgactggatt tttcaacctg ttcctctatg atctgttcca ctgctccgga
     6841 atatgcgcca tcagaggcca ggaaaaaatt ttcactggaa cggattgccc gataagtatc
     6901 catcctggca tcacagtaca gacaatccga gaaattactg ccatgctgat taagcagctt
     6961 taacagctga tatccctgat gcttttctgc agcagcttct tcccttttta catgattctt
     7021 attgatatac atgtttttgt ctgcgcagtt aaacagttct ctcattgtac ttctgggaaa
     7081 atcgccggcc agtgcaaagc ctgctgcata gctcagggga gtatccggat acactttgga
     7141 ttcctctctc atatctctgc gcaccttttc aaggcactgt gtcatctgtt ccctgtccag
     7201 tccatgggta actgcaagaa actcatctcc acctgctctt cccacaaact gctctgccgg
     7261 catggaagcc cgcaggcaga tggcaaatct acggatatag gcatcaccag cttcatgacc
     7321 tctgctgtca ttaatacgcc ggagattatt aagatcaaaa ctgcagacac cggtatctgc
     7381 atccggttct tcctcactca gtaattcttc acatttattt ttgttcggaa gtcccgtcgc
     7441 atcatccaga tatacctttc gctgcagtaa acgattcatt gcagcatatt gtattgcttt
     7501 gatgaattca tatccgatca gcagcatcaa cacaacaata tctgctgtga tatatttttc
     7561 aagcaatgat aaggatgtgg cttttttctg cgattatttt tcggcca
//
LOCUS       DBMDXG010000056         7450 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2583_length_7450_cov_1.660498, whole genome shotgun sequence.
ACCESSION   DBMDXG010000056 DBMDXG010000000
VERSION     DBMDXG010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2583_length_7450_cov_1.660498"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="ACZPD3_02860"
     CDS             complement(<1..1083)
                     /locus_tag="ACZPD3_02860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR1414980.1"
                     /translation="MMNKDNRNDKIRKIAKKGLTFSLCAVLAGGLAAGSFEGVNKLAG
                     WSGATTVEADSNKDETTLTYAKSEKKDADASDSKSDTGKDTGSTAKGSLDVSEIVSEA
                     LPSIVSITTKSVQEVQNYFGMYGMYGYAPQQQEQEVEGSGSGIIVGKNDDELLIATNY
                     HVVEGADTLSVAFTDGNAVEASVKGFDEERDLAVVSVSLDDVEDDTMDAISIANIGSS
                     DDLKVGEQVVAIGNALGYGQSVTTGIVSAKNRRMDSDNNTVTDGSDDSSDGVNLIQTD
                     AAINPGNSGGALLNMEGEVVGINSAKLASTEVEGMGYAIAISDVTDILQNLMNETSRD
                     KLDDSEHGVLGIKGSSVSSEAVQMYGI"
     gene            complement(1272..3761)
                     /locus_tag="ACZPD3_02865"
     CDS             complement(1272..3761)
                     /locus_tag="ACZPD3_02865"
                     /inference="COORDINATES: protein motif:HMM:NF040163.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5722 domain-containing protein"
                     /protein_id="HIR1414981.1"
                     /translation="MKRWKKFIAMGLAVSMTVPSCIPQTLWASEFSAGSVESAADVFE
                     DGAGYETGSESGNKISDENNSGDEFGTGELKDQEPEQSETEDSVNAGQSAEGYNILLY
                     SDGKLEKTYVIPYADADTAKVPEALKGKTGYIFKEWNTKEDGTGETYKPGDSIKKLLE
                     TADMAKQAQETSERTEAGEEIKPEKDVMQEENDVAQTDSASWEEKTDKAEMQISDTEN
                     DGTSPDISTDKAIAAGDTTAITLYAIWEKASEYKITYKLNKGKNNTANPKTYTSEDEI
                     KFKKPTRSGYHFVGWYTDSKYKNQISVIEKGSEGSLTLYAKWTKEISPSAKAASLDYV
                     KGTKVNTITVSATVSNYVKSSDGYYYLVYVDSNSGKVKKTVGKVKKPEKAKGKITFKL
                     NISGHPEYAQGKFAIGIKKSKSAYSVISPKSYVSNPEKLSTNTAAYFVPGTKKGIQAT
                     DINELTDTKSKTVFFNLYISDLMRKDSGVETYKYNGKTYHFNGLYGYVYLVQQCNAKG
                     IQVTAQISIDRNASTQSFITGNSPYAETAYYGWNTDNSTTRQTMEAMFAYLGEKFGKN
                     NCYISNWILGNEVNSASGYYYVGNVSFSKFISMYSEAFRCLYNAVKSSRGSSKVFICL
                     DNCWNQKNAFTICYSARSTLESFAAKISDMQKDVNWNLAYHAYNQPLSDSQFWSGANA
                     SMFTSDANTTTFITMRNIQTLTDYVKNRFGSNTRIILSEQGFSSTYGGQANQAAAIAL
                     AYYKAACNPMIDAFIIRSYKDEAHEVAQGLAMGLKDANGKKKTAYNVFKNMDSSNSLK
                     YTEKVLKSQVGNWKSLVPGYSTGKISSMYRK"
     gene            complement(4127..4696)
                     /gene="lepB"
                     /locus_tag="ACZPD3_02870"
     CDS             complement(4127..4696)
                     /gene="lepB"
                     /locus_tag="ACZPD3_02870"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1414982.1"
                     /translation="MENEEKNQTEQSDKSRSVWRELWDYAKIIIAVFVIAFLLGHFVY
                     INARVPSGSMEETIMTGDRVFGNRLAYIKDDPERFDIVIFKYPDDPSQLFVKRVIGLP
                     GETVNIVDGKVYINDSEEPLDDSFCPETPEGSFGPYTVPEGCYFMLGDNRNHSMDSRY
                     WQNPFVEEDAIEAEVAVRYWPLNKIGTVK"
     gene            complement(5483..7042)
                     /locus_tag="ACZPD3_02875"
     CDS             complement(5483..7042)
                     /locus_tag="ACZPD3_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR1414983.1"
                     /translation="MNNIFAERLKKAMEQKNMKQIDLVKKAAEQGVKLGKSHVSQYLS
                     GKTTPRSEILNFLATTLGVETEWLKGTDVSVDTLKKETNEAGIQMENMKFDYNYNNMK
                     ENTRETVEEVQVREFKKSSKLNNVLYDVRGPVVEEAARMENAGTQVLKLNIGNPAPFG
                     FRTPDEVIYDMRQQLTECEGYSPAKGLFSARKAIMQYAQLKKLPNVSIEDIYTGNGVS
                     ELINLCMSALLDNGDEILIPSPDYPLWTACATLAGGKAVHYICDEQAEWYPDMDDIRK
                     KITSRTKAIVIINPNNPTGALYPREILQQIVDIAREHHLIIFSDEIYDRLVMDGAEHI
                     SIASMAPDLFCVTFSGLSKSHMIAGFRIGWMILSGNKAVAKDYIEGIKMLSNMRLCSN
                     VPAQSIVQTALGGHQSVESYIVPGGRIYEQREFIYNALTDIPGITAVKPKAAFYMFPK
                     IDTKKFNIVNDEKFALDLLKEKKILLVHGGGFNWHQPDHFRVVYLPRIEVLKEAAGKI
                     RDFLEYYQQEI"
BASE COUNT         1996 a         1692 c         1155 g         2607 t
ORIGIN      
        1 aatgccatac atctgaactg cctcagaact tacagaagaa cctttgattc caagtacacc
       61 atgttcagaa tcatccagtt tatctcttga tgtctcattc atcagattct gaagaatgtc
      121 agtcacgtct gagattgcaa ttgcataacc cataccttct acttcagtag aagcaagttt
      181 tgcagagttg ataccaacaa cctcgccttc catattcaga agagcaccgc cactgttacc
      241 agggttgata gccgcatctg tctggatcag gtttacaccg tcactgctgt catcacttcc
      301 atctgttact gtgttattgt cagaatccat tctgcggttc ttggcactta cgataccggt
      361 tgtaacagac tgtccatatc caagagcatt accgattgca actacctgct cgcccacctt
      421 cagatcatca gaactgccaa tattcgcaat tgaaatggcg tccattgtat catcttcaac
      481 atcatccaga gatacagata caactgcaag atctctctct tcatcaaatc cctttacgga
      541 tgcctccact gcatttccat ctgtaaatgc aactgacaat gtatccgcac cttctactac
      601 atgataattt gtagcaatca gaagttcatc atcattctta ccaacaataa taccggaacc
      661 actgccctct acttcctgtt cctgctgctg tggcgcatat ccatacattc catacattcc
      721 gaagtaattc tgtacctcct gaactgactt ggttgtaata gatacaatag atggaagtgc
      781 ttctgatacg atttcagaca catcaaggct tccttttgct gtgctacctg tatctttgcc
      841 ggtatctgat ttggaatcac tggcatctgc atccttcttc tcggattttg catatgtcag
      901 ggttgtctcg tccttatttg aatcagcttc taccgttgtg gctccgctcc atcctgcaag
      961 tttatttact ccctcaaagc ttcctgctgc cagtcctcct gcaagtactg cacataagct
     1021 gaatgtaagg cctttctttg caatctttct gattttatca tttctgttat ctttgttcat
     1081 cataataaag tccctccatg aatctatgtc atttatctat gtcatttatt tatctctgtt
     1141 tccttttgat gcttctaagt ataggaatga tttgtgttct ttgtgtggga aaaatgtgtt
     1201 taatttgtga caattttata catttccaat atgacagcaa tttttaaaaa cagtcctgtc
     1261 tgtcagttac atcactttcg atacatggag gaaatttttc cggtactata tcccggaacc
     1321 agtgatttcc agtttccaac ctgacttttt agtacctttt ctgtatattt cagggaattg
     1381 gaactatcca tatttttaaa gacattatac gctgttttct ttttcccatt ggcatccttc
     1441 aatcccattg ccaatccctg agccacctca tgggcttcat ctttatagct tcttataata
     1501 aatgcatcaa tcattggatt gcaggcagcc ttataatagg ccagtgcaat tgctgccgcc
     1561 tgatttgcct gtcctccata agtggaagaa aatccctgtt ccgaaagaat gatccttgta
     1621 ttggaaccaa atctattttt cacataatct gtaagtgtct ggatattacg catagtgata
     1681 aacgtggtgg tgttcgcatc actggtaaac atggatgcat ttgcacctga ccagaactgt
     1741 gaatctgaaa gcggctgatt atatgcatga tatgccagat tccagttcac atctttctgc
     1801 atatcagaaa tcttcgccgc aaagctttcc agagttgatc ttgcactata gcagatagta
     1861 aaggcatttt tctgattcca gcaattatcc agacagataa atactttgga acttccccgg
     1921 ctgcttttca ctgcattgta aaggcagcga aaagcttcac tgtacatgga gataaatttt
     1981 gaaaaactga cattccctac ataataatat ccgcttgcac tgtttacttc atttcccagg
     2041 atccagttac ttatataaca gttatttttt ccaaacttct ctcccagata tgcaaacatt
     2101 gcttccatag tctgtctagt tgtgctgtta tctgtgttcc atccataata agctgtttct
     2161 gcataaggac tgtttcccgt aataaagctc tgcgtactgg cattcctgtc aatactgatc
     2221 tgtgcagtca cctggattcc ctttgcatta cattgctgaa caagatatac atatccgtac
     2281 agaccattaa aatgatatgt tttcccatta tatttatatg tttccacacc actgtctttt
     2341 cgcatcaggt ctgaaatata taagttaaaa aatactgttt tggattttgt atctgtcagc
     2401 tcgttgatat ctgtagcctg aattcctttt tttgtccccg gaacaaaata tgctgctgta
     2461 tttgtactga gtttctccgg attgctgaca tagcttttcg gactgatcac actatacgca
     2521 gatttagatt tttttatccc tatggcaaat tttccctgtg catattccgg atgtccggaa
     2581 atgttcagct taaatgtgat cttccctttt gccttctccg gcttctttac tttacccacc
     2641 gtttttttca ctttaccgga attagaatct acatacacca gatagtaata accatccgaa
     2701 cttttcacat aatttgaaac agttgcggat acagtgatgg tattcacctt tgttcctttt
     2761 acataatcca gtgatgcagc tttcgccgaa ggactgattt cctttgtcca ttttgcatat
     2821 aaagtaaggg aaccttctga acccttttca atcacactta tctgattttt atacttactg
     2881 tctgtatacc atcccacaaa atgatatccg gaacgcgtcg gctttttaaa tttaatctca
     2941 tcttctgaag tataagtttt gggattagca gtattatttt ttcctttatt aagtttatat
     3001 gtgattttat attcggatgc tttctcccag attgcataca gggttattgc tgttgtatct
     3061 cctgctgcaa tagccttatc tgtagatata tccgggcttg ttccatcatt ttctgtgtct
     3121 gatatctgca tttctgcctt atcagttttt tcttcccatg atgcgctgtc cgtctgtgcc
     3181 acatcatttt cttcctgcat cacatctttt tctggtttta tttcttctcc ggcttctgtc
     3241 ctctcactgg tttcctgagc ctgctttgcc atatctgctg tttcaagaag tttcttaata
     3301 gaatctcccg gtttataagt ttcaccggtt ccatcttctt tcgtattcca ctctttaaaa
     3361 atgtaacctg tctttccctt cagtgcttcc ggtactttag cagtatccgc atctgcatag
     3421 ggtatcacat atgttttttc cagtttacca tcactgtaaa gcagaatgtt ataaccttcc
     3481 gcactctgtc cggcatttac tgaatcttct gtctcagact gttccggttc ctgatctttt
     3541 aactctcctg ttccaaactc atctccactg ttattctcat cagatatttt atttcctgac
     3601 tcacttcctg tctcatatcc ggcaccatct tcaaaaacat ctgctgctga ttcaacactt
     3661 cctgccgaaa actctgaagc ccacaatgtc tgcggtatac aggacggaac tgtcatagat
     3721 accgccagtc ccattgctat aaactttttc catcttttca tattcgtctt cctttctctt
     3781 tttacatttt acggaattgt tgtaactatc cagtattcca ttttcttcat tttcctcctg
     3841 tcttacagta taacatttga aaaatccaca gacaacaaaa gactgtcaaa atcaggacac
     3901 taatatgtga ttttcctgat tttaacagtc ttttataaaa tttccaaact acctgtaact
     3961 acccggcagt ttaaccgaat caagtaagtc ccctgcggag gttgcgaaaa gcaataagca
     4021 gtgggtgggg acttgcttgt atcaagatga gtgcaagctc cttctgataa actgcgaagc
     4081 ggatgatttt atccgcttaa cttttgaaaa tatggaaccg tcacgattat tttacagttc
     4141 ctatcttatt taacggccag taacgaactg ctacttctgc ctctattgca tcttcctcta
     4201 caaacggatt ttgccagtat ctggaatcca tactgtggtt tctgttatct cctaacatga
     4261 aataacatcc ctccggaact gtatacggtc caaagcttcc ttccggtgtt tccggacaaa
     4321 agctgtcatc cagcggttct tcggaatcat tgatatacac ttttccatct acaatgttta
     4381 ctgtttctcc cggaagacca attactcgtt ttacaaaaag ctgagatgga tcatccggat
     4441 atttaaatat tacaatatca aatctctccg gatcatcttt tatatatgcc agacgatttc
     4501 caaatactct gtcaccagtc ataattgttt cttccataga acctgacggc actcgtgcat
     4561 taatatatac aaagtgtccc aacagaaacg ctataacaaa gactgcaatg ataatcttcg
     4621 catagtccca caactctctc cacacagacc tgcttttgtc tgactgttct gtctggtttt
     4681 tttcctcatt ttccattttt cgttatacct ccagaaattt atatcacagc atcatttgat
     4741 gatatcaggg gcaaaatact tttaaaccta acctcattta ctgttatcag aataataact
     4801 atttcgcaaa taacgcatta aacatagttt atcacatctg atagttattc accacttaat
     4861 tttttataaa attttgtcac tatttgattc ttggttattg ttcactccgt tctcagcaac
     4921 acgcttcgcg atgcaggaaa tatgaacctc cttgtgcaat gcccaaggag tatcgtttgc
     4981 ttcgcaaatt taatttatgc taatcgcata aattcgcgcg gtatgtcttg ggttttctgt
     5041 gaccttcgct cacaaaaaac acctcacggg atattaccag tgaacagtaa cgattcttgg
     5101 acaatcttgt cagcacatta tgtttcacgt gaaacaaaca tattgtcttc ctgtagtaat
     5161 tccacaattt aaccgaatca attaagtccc ctgcggaggt tgcgaaaagc aataagcaat
     5221 gggtgaggac ttgcttgtat caggagaagt gtaaactcct tctgataaac tgcgaaacag
     5281 ttattcggtt atgaacaagc agcgaagcgg atgattttat cagcttgacc cgagtcaaac
     5341 aaatctactg cagcacaatt attcgatgta gagctgcgta gcaaaatgaa tgattttatc
     5401 cgtttgacca aaagttttta taatatcagg aaatgggtac tatgtttcac gtgaaacata
     5461 gtacccattt ctcactgaca gattatattt cctgctggta atattccaga aaatctctga
     5521 ttttgccggc tgcttctttt aatacttcta ttcttggtaa ataaaccact ctgaagtgat
     5581 ccggctgatg ccagttaaat ccaccgccat gtacaagcag aatcttcttc tcttttaata
     5641 aatctaatgc aaatttctca tcatttacga tattaaattt ctttgtatca atcttaggga
     5701 acatataaaa tgcagctttc ggtttcacag cagtaattcc cggaatatct gtcagcgcat
     5761 tataaataaa ttctctctgc tcataaatac gtccaccagg aacaatataa ctctccacac
     5821 tctgatgtcc gcccaatgca gtctgaacaa ttgactgcgc cggtacatta gaacaaagtc
     5881 tcatattaga aagcatcttg attccttcta tataatcttt tgcaactgcc ttattgccac
     5941 tgagaatcat ccatccgatt ctaaagcccg cgatcatatg tgatttggat aaaccgctga
     6001 atgttacaca gaataaatcc ggagccattg aagcaatgga aatatgttct gctccgtcca
     6061 ttacaagacg gtcatagatt tcatcagaga aaatgatcag atgatgttct ctggcaatat
     6121 ctactatctg ttgtaatatc tctctcggat acaacgcacc agtcgggtta ttcggattta
     6181 taataacgat tgcttttgtt ctgctggtaa ttttctttct gatatcatcc atatccggat
     6241 accattctgc ctgctcatca caaatataat gtactgcttt tccaccagct aaagttgcac
     6301 atgcagtcca caacggataa tccggagatg gaatcaaaat ctcatctcca ttatccaaca
     6361 gagctgacat acagagatta atcagttcac tgacaccgtt tcctgtataa atatcctcaa
     6421 ttgatacatt agggagcttt ttcaactgtg catactgcat gatcgctttt ctggcagaaa
     6481 acaaaccttt tgcaggagaa tatccctcac actctgtcag ctgctgtctc atatcataaa
     6541 tcacttcgtc cggtgttctg aatccaaatg gtgccggatt tccgatattc agtttcaaaa
     6601 cctgagttcc tgcgttttcc atacgtgcag cttcttctac aaccggtcct cttacatcat
     6661 ataatacgtt atttaattta gatgattttt taaattcacg aacctgtact tcctcaactg
     6721 tctccctggt attttctttc atattattat aattgtaatc aaatttcata ttttccattt
     6781 gaattcctgc ctcatttgtc tctttcttta aagtgtctac agatacatcg gttcctttca
     6841 gccattcagt ttctactcct aatgttgtcg ccagaaaatt aagaatttct gatctgggag
     6901 ttgtttttcc actcagatac tggctcacat gactctttcc aagtttcacg ccctgctctg
     6961 ccgccttttt tacaaggtcg atctgcttca tattcttctg ttccattgct ttttttaatc
     7021 gttcagcaaa tatattattc ataaatacta tctccgttct ctgtaaaaaa caaattacat
     7081 tgttataatc aatggttatt gttatatttc ttaattcttt cgcatatatg ttatagaaag
     7141 aacagatata ttatattgtt tatacggaat caaaatacat tcagacatac aaacattaat
     7201 taagttcaaa tgtatttctt tcagcatcat acgcgaatat atgttctgtt catttttaaa
     7261 tcccgcattt tatcagcttt ttcaagtttt tttctttgct attatactat aattgaacta
     7321 ataattcaac ggtaaatttt attattttca atttcatcag taaagttcaa tttttagttt
     7381 attttatagt tcattatcaa tcgaactata ttgaacatat taattgcttt cgttacactg
     7441 gttcaattag
//
LOCUS       DBMDXG010000057         7414 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2597_length_7414_cov_3.002735, whole genome shotgun sequence.
ACCESSION   DBMDXG010000057 DBMDXG010000000
VERSION     DBMDXG010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2597_length_7414_cov_3.002735"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            405..878
                     /locus_tag="ACZPD3_02880"
     CDS             405..878
                     /locus_tag="ACZPD3_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1414984.1"
                     /translation="MYIEHPYFYEGKYYANIDGEMIEITKEVAYAMNNFYRSSKAKKV
                     EIKNELGEVVDKMLREVPYSGQSIDGEGFMIEDFPDLNCDVEHSVLTKMEQQDIHKVI
                     NQLNSEERMIIYGIFFENKTQTQMAEIMGISRQMLSYKLKSILNKMREMYMNKFF"
     gene            1453..1833
                     /locus_tag="ACZPD3_02885"
     CDS             1453..1833
                     /locus_tag="ACZPD3_02885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR1414985.1"
                     /translation="MTIRRGDILWADLGMFPTTSVQGGVRPVIVVSNNKANTYSSVIT
                     VVPLTSRIYKKRYLPTHVFISKYDMTGIRKGSLALAEQVMSISTKCIIEKCGRVNKWS
                     LDRVLKAVRIQMGMEGEGHDSRGI"
     gene            1814..2062
                     /locus_tag="ACZPD3_02890"
     CDS             1814..2062
                     /locus_tag="ACZPD3_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6870 family protein"
                     /protein_id="HIR1414986.1"
                     /translation="MTAEEYRNYLDQDFSDVDINEMTDLRMIKTDRNKSLQERRDIFL
                     NKVGNPYLVRIGNMKVKVRFANNGISMEQAFENMLLSV"
     gene            2171..3862
                     /locus_tag="ACZPD3_02895"
     CDS             2171..3862
                     /locus_tag="ACZPD3_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1414987.1"
                     /translation="MYQNINKIYHAAIYVRLSKEDGDISSSAKLESNSISNQKALILD
                     FLKDKKDIEVVSVRVDDGYSGSNFERPAFQAMLEDIRHGIVDCVVVKDLSRFGREYID
                     SGKYIERLFPALGVRFIAINDNYDSLKGKNQADEIIIPFKNLINDAYCRDISIKIRSN
                     LEIKRKKGECVTPFVAFGYRKTKTDKHKLEIDPSAGGVVQDIFKMKLQGMSQDAIANR
                     LNELGILSPFEYKISSGSRYETGFRQKEQALWSSVTVRRILENEVYIGNLVQGKRTTP
                     NHKVKQTYVKPEDDWIRIEKNHEPLVSDRDFEIVQRLLGMDTRTSPDQKQVYLLSGIA
                     VCADCGAPMTRKVSTVAGKKYAYYLCSTNKETKRCSSHRIPEKDLEDAVLVMLKQHIQ
                     NILHLKRVLEFIGTVPFQEINMKKLQDRLEKKKQEKERCKELRMMLYSDMKEGIVSKE
                     DYVELHAAYGKRLRNAEESIRAIQKEMDNELEKADKANTWLDYFVKYQDIEELSRTVV
                     VELIRQIRVYDKKNIEITFDFDDCYQTLLNQLPAMGVDMVIDDDNNLQVNVKEVV"
     gene            3864..5555
                     /locus_tag="ACZPD3_02900"
     CDS             3864..5555
                     /locus_tag="ACZPD3_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1414988.1"
                     /translation="MARKSRKNIPEVVSSATVQTEVSRPFRAGLYARISMETEETLER
                     GTIETQVELMKNFVADTEDIAVAEIYKDSDYSGTNFDRPGFTQMMEDIKHGKINCVIV
                     KDLSRLGRNYVETSNYIERVFPFFHVRFLAVTDDFDSFREGVDLTVPLKNIINEFYSK
                     DLAKKSSSAKKALWKEGKFTGAWEPYGYRKSEQDRHQLVVDEEAAGHLREIFTMYMDG
                     CSYSDIAKRLNTDGILSPTLQREYYKTGEKPSPESKPWNNYEVKRVLQDVHCTGDSVY
                     GKYQQSVFQGNKQRNLPESEWLYAENTHEGIIDKELFRQVQEKIREFTEAYKKKHQLN
                     NGAIRNHNFYTGKIRCGGCGNRMVLSREKTGTFYYICGADANHKSGGNQCKGHRVKKE
                     YVDEDVLRLIRSHMKAVLDTEKLIREMNSASRNQTQYLLLDKEVGKLRRELSRISKRK
                     SDLYEDYSERLITEEEYMQFSRIYSNEIENIKNRLDVVLAAQVRYSKDYHIEEGWGKV
                     IHTYMSKRKLTKEMADAFVDSIIIHGKYDYEIKLVYDDQFADLQKLKKEKEAQSR"
     gene            5552..7129
                     /locus_tag="ACZPD3_02905"
     CDS             5552..7129
                     /locus_tag="ACZPD3_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1414989.1"
                     /translation="MTDHRKTAVYIRLSAEDDNVDGRAKKESDSVTSQRILLKSFVID
                     QLGVDEADILEYVDDGVSGTHFKRQGFQQLQDDMKSGEIGCVVVKDFSRFGRDYLEVG
                     FYIEYIFPLLQIRFISINDSYDSAASSGMTGGMNVALQNLVYNMYSLDLSKKISSAMQ
                     TRTKNGTRLPVNARYGYKKGKDGRLEVDPEAAKVVKMIFQMAAEGTSFADITRELNRQ
                     AIATCDEQKLSRGDQVQFQRFDTIKKKHWSPTTVSAIVRDEIYIGTRIWGKTRCSMHT
                     GHKAVLNDETEWVRLENHHTAIIDRELFEKANEMHPKKKRSVAESRTNFTLERRKKQP
                     ALLICANCGHSLLKETEHLLKCSDARTNGDPVCRSLVIRREPMEENILGLVRQYAASM
                     LKKGKKVSSKRQCEYKEINTTELQKQSRQLTSEKMKLYDDYKDGRIDRDSYKQRAEKI
                     SVQLDEIKRKIEDAKNSKKLLEQNELSDKIKLKDFLGIQKFDTEKLREVIKVIRVHSQ
                     DEIEIEWNFDDIFSEQR"
     gene            7251..7414
                     /locus_tag="ACZPD3_02910"
                     /pseudo
     CDS             7251..7414
                     /locus_tag="ACZPD3_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2542 a         1161 c         1824 g         1887 t
ORIGIN      
        1 acggaaagga ctatgatgta gtcaataatc ctgataaatt tcaactatat atagatggaa
       61 aagaagtaga agaataacac ggtgaacttt ttatactgaa atatgtacga tttcaataat
      121 gatgtgaact tataggaatg tattgcggat actttgtgaa ctttatcctg atacaatgat
      181 tatgctgatg aaagaaggca tagataactg aatataagaa agagacgaag gcctcagtag
      241 ttaagaaaaa aactactggg gctttttttc gtgcctgaat ttctcagcca ataactgcgg
      301 gtatgtcccg gcaggataaa ggcggagcag gcatgattct aaacagaggt ccgccttctg
      361 gatttgaaac aagcaacgag atttcaaatt caggaggaac ggacatgtat attgaacacc
      421 catattttta tgaaggaaag tattacgcaa atattgatgg agagatgatt gagattacaa
      481 aagaggttgc gtatgcgatg aacaattttt atagaagcag caaggcaaaa aaggtagaaa
      541 tcaaaaacga attgggagag gtcgtggaca agatgttgag ggaagtacct tacagtggtc
      601 agtccattga tggagaaggc tttatgatag aggattttcc ggatctgaat tgtgatgtgg
      661 agcatagtgt gctgacaaaa atggaacagc aggatatcca caaagtgatc aatcagctga
      721 actccgagga acgcatgatt atttacggta ttttctttga aaacaagaca cagacacaga
      781 tggcagagat tatgggaatt tccagacaga tgctgtcata caagctgaaa tccattctta
      841 ataagatgcg tgaaatgtat atgaataaat ttttttaaaa aatttttcaa aatcttttgc
      901 atcttccgaa gtcatttgac tttagtaatt agaggagctt taaggactcc ctaaaacgca
      961 ccgtgataac tgaatatcca ataataactg acgtgacatt tcatacagtg agcacgagga
     1021 agatgccgct ataggattat aagacgtaaa aacattttta gagctaataa tgctgcgaaa
     1081 aagcactgac caaagccagt cggcaggctg gtgatgcgat gacatgtgtg agatgcgata
     1141 cacttgcaga ccctgagaag tctgtaattg gtaaacctat gaactgccag agcctggaaa
     1201 cgagagtttt tgggttgtgg tccggagtat agcattcgga caggttatta ttcccttcct
     1261 gcagatgcag gagtaacgat tcggggtatc gaggataaat agaatcgagc gagcactgtt
     1321 taataatcaa attggttata gaggttatgc ggcagagctt tccatcttgg aaaggaactc
     1381 tgccgtattc ctgtggagcc aatatagcgc tagggatgct ttacaaataa aaaaagattg
     1441 gagagatgac aagtgacgat tcgaagagga gatatcttat gggcggatct cggtatgttt
     1501 ccgacaactt cggttcaggg tggagtaaga ccggtgatcg tagtaagtaa caacaaagct
     1561 aatacataca gttcagtcat tacagtagtt ccgctgacgt caagaatata taagaagcgg
     1621 tatttgccaa cacatgtatt tatcagtaag tacgatatga cagggatccg aaaaggaagc
     1681 ctggcattgg ctgaacaggt tatgagtatt tctacaaaat gtattattga gaaatgcgga
     1741 agagtaaata agtggagcct ggatcgggta ctgaaagcag tacggattca gatgggaatg
     1801 gaaggagaag ggcatgacag cagaggaata tagaaattat ctggatcagg atttcagtga
     1861 tgtggatatc aatgaaatga ctgatctacg gatgataaaa acagacagaa ataaatcgct
     1921 tcaggaacgg agagatattt ttctgaataa agtaggaaat ccttatctgg tccggatcgg
     1981 taatatgaaa gtaaaagtca gatttgcaaa taacggtata tctatggagc aggcatttga
     2041 gaatatgctt ctgagtgtct gaaaaagtac tggtaaaatt gaggggactg tgttagaatg
     2101 tcggtaagga caaattatgc agcccccttt tattacatgg attttctgac gtaataaaag
     2161 gaggtaagat atgtatcaga atatcaacaa aatctatcat gccgccatct atgtcagatt
     2221 atctaaagaa gatggcgata tttccagttc agcaaaactg gaaagtaaca gcatttctaa
     2281 ccagaaagct ttgattctgg atttcctgaa agacaaaaaa gatattgaag ttgtttcagt
     2341 ccgagttgat gatggctact caggctctaa ttttgagcgc cctgcattcc aggcaatgct
     2401 ggaagatatc cggcatggga ttgtagactg cgtagtggta aaagatttat cacgatttgg
     2461 aagggaatat attgattccg ggaagtatat cgagagatta ttcccggctc ttggtgtgcg
     2521 ttttattgcc atcaatgata attatgacag tcttaaggga aagaatcagg cagacgaaat
     2581 cattatccca tttaaaaatt taatcaacga tgcttattgt cgtgatattt ctattaagat
     2641 caggagtaat cttgagatta agaggaaaaa aggcgagtgc gtgactccgt ttgtagcatt
     2701 tggatacagg aagacaaaga cagataagca taaattagag attgatccat cggcgggcgg
     2761 tgtagtacag gatattttta agatgaagct gcagggaatg agtcaggatg cgatcgctaa
     2821 tcgtttaaat gaactgggta ttctttcccc atttgagtat aaaataagca gcggcagtcg
     2881 ttatgaaaca ggtttccggc agaaagaaca ggcactttgg agttccgtta cggtccgcag
     2941 gatactggaa aacgaggttt atatcggaaa ccttgtacag ggaaaaagga caactccaaa
     3001 ccataaagtg aagcagactt atgtgaaacc ggaagatgac tggatccgga ttgagaaaaa
     3061 tcatgaacct ctggtgagtg accgtgattt tgaaattgtt cagaggcttc ttggcatgga
     3121 tacacgtact tcacctgacc aaaagcaggt atatctgtta tccggaattg ctgtatgtgc
     3181 ggattgtgga gcacctatga caagaaaggt ttctactgtg gcaggaaaaa aatatgctta
     3241 ttatctatgt tccacaaata aagaaacaaa acgctgttcc agtcatagaa taccggaaaa
     3301 ggatctggaa gatgccgtat tagtgatgtt gaaacagcat atccagaata tcctgcacct
     3361 gaaaagagtc ctggaattta tcggtactgt gccatttcag gagatcaata tgaaaaagct
     3421 gcaggacagg ctggagaaga aaaagcagga gaaagaacgc tgtaaggagt tacggatgat
     3481 gctctattcg gatatgaaag agggcattgt atcaaaagaa gactatgtag agcttcatgc
     3541 agcatatgga aagcggctta gaaatgcgga agagagtatc cgtgcgatcc agaaagaaat
     3601 ggataacgag ctggaaaaag cagacaaagc caatacctgg ctggattatt ttgtgaaata
     3661 tcaggatatt gaagaactgt cccgcactgt ggtggtagaa ctgatcaggc aaatcagagt
     3721 atatgataaa aagaatatag agatcacttt tgattttgat gattgttatc agacattgct
     3781 gaatcaatta cctgctatgg gagttgatat ggtaatagat gatgataaca atctgcaggt
     3841 caatgtaaag gaggttgtat aaaatggcac gaaagagcag gaaaaacatt ccggaagtag
     3901 tcagcagtgc aaccgtccag acagaggtga gcagaccgtt ccgggcagga ttgtatgcca
     3961 gaatttcaat ggaaacagaa gaaaccttag aaagaggaac gatagaaacc caggtggaac
     4021 tgatgaaaaa ctttgtggca gatacggaag acatcgcagt tgcagaaata tataaggatt
     4081 ctgattattc tggtacaaat ttcgaccgtc ctggttttac gcagatgatg gaagatatca
     4141 agcatggaaa aattaactgt gtgattgtaa aggatctatc cagacttgga aggaattatg
     4201 tagagacaag taattatatt gagcgtgtat ttccgttttt tcatgtgcgg tttcttgctg
     4261 tgacggatga ctttgattct ttccgtgagg gagtggatct gacagttccc ttaaaaaata
     4321 tcatcaatga gttttattcc aaagaccttg caaaaaagag cagcagtgcc aaaaaagccc
     4381 tgtggaaaga aggaaaattt actggtgcat gggaacctta tggatacagg aaatcagaac
     4441 aggacagaca ccagctggta gttgacgagg aggctgccgg acatttacga gaaattttta
     4501 ctatgtatat ggatggatgc agctacagcg atattgcaaa aagactgaat acagatggta
     4561 tcctttcccc gactttgcag agggaatatt ataaaacagg cgaaaagcct tcaccggaat
     4621 ccaaaccttg gaataattat gaagtgaaac gtgtacttca ggatgtccat tgtaccggag
     4681 attctgtata tggaaaatat cagcagagtg tttttcaagg aaacaagcag agaaatctac
     4741 cggagagtga gtggctctat gctgagaata cccacgaagg aattattgat aaagaactgt
     4801 tccggcaggt acaggaaaaa atccgggaat ttacggaagc atacaaaaag aagcatcagc
     4861 tgaataatgg agccattcgg aatcataatt tctataccgg caagatcagg tgtgggggtt
     4921 gtgggaaccg catggtgttg tccagagaaa aaacagggac attttattat atctgtggtg
     4981 cagatgcgaa ccataaatcc ggaggaaatc agtgtaaggg gcacagagtc aaaaaagaat
     5041 acgtggatga agacgtcctt cgcctgatcc ggtcacatat gaaagccgta ttggatacag
     5101 agaaactgat ccgtgaaatg aattctgctt ccagaaacca gacacagtat ttgcttctgg
     5161 ataaggaagt ggggaaactt cggcgggagt tgagcaggat cagcaagcgt aaatctgatt
     5221 tatatgagga ttattcggaa cgtctgatta cagaagaaga atacatgcag ttttcccgta
     5281 tctattccaa tgaaatagaa aacatcaaga accgcctgga tgtagtattg gcggcacagg
     5341 tacggtattc caaggattat catatcgagg aaggatgggg aaaagtgata catacttata
     5401 tgtcaaagcg taagctgaca aaagaaatgg cagatgcctt tgtggattca attattatcc
     5461 atggtaagta tgattatgag atcaagctgg tatatgacga tcagtttgca gatttacaga
     5521 aattaaagaa agagaaggag gcgcagtcaa gatgacagat catagaaaaa cagctgttta
     5581 catccgttta tccgcagaag atgacaatgt agatggcaga gctaaaaaag agagcgacag
     5641 tgtaacttct cagagaattc tgttaaaatc atttgtgatt gatcagctgg gtgtagatga
     5701 ggcagatatc ctggaatatg tggatgatgg tgtcagcggt actcatttta aacggcaggg
     5761 atttcaacag ctgcaggatg acatgaaaag tggagaaatc ggctgtgtag ttgtaaaaga
     5821 cttttccaga ttcggaagag actatctgga agttggattt tatattgaat atatttttcc
     5881 acttctgcag attcgattta tttcgattaa tgacagctat gacagtgcag caagcagcgg
     5941 aatgactggt ggaatgaatg tggcattgca gaatctggta tacaatatgt atagcctgga
     6001 tttgtccaag aaaatttcat cagcaatgca gacgagaaca aaaaatggta cacgtcttcc
     6061 ggtaaatgcc agatatggct ataaaaaagg aaaagatgga aggctggaag ttgatccgga
     6121 ggctgcaaaa gtcgtgaaaa tgatttttca gatggcagcg gagggaacaa gttttgcaga
     6181 tattacaagg gaattgaaca gacaggcgat tgctacttgt gatgagcaga aattatcaag
     6241 aggggatcag gtgcagttcc agaggtttga cacgatcaaa aagaaacact ggagccctac
     6301 gacagtatcg gcaatcgtcc gggatgagat ttatattgga accagaatct ggggcaaaac
     6361 acgttgcagt atgcatacgg gccataaagc agttctgaat gatgaaacag aatgggttcg
     6421 cctggaaaat catcatacgg caattataga cagggaattg tttgaaaaag caaatgaaat
     6481 gcatccgaaa aagaagagaa gtgttgcaga atcacgcacc aattttactc tggaaagacg
     6541 taaaaaacag ccggccttgc tgatatgtgc caactgtggg cattctctgt taaaagaaac
     6601 agagcatctg ctgaaatgtt cagatgccag aaccaatggt gatcctgtgt gccgaagtct
     6661 ggtgattcgt agggaaccga tggaggagaa tatccttggg cttgtccgtc agtatgcagc
     6721 gtcaatgttg aaaaaaggaa agaaagtatc ttccaaaaga caatgtgaat acaaagagat
     6781 caatactaca gaattgcaaa aacagagccg acagttgacc tcggaaaaga tgaaactcta
     6841 tgatgattac aaagatggtc gtatagatcg tgattcgtat aagcagagag ctgaaaagat
     6901 aagtgtacag ctggatgaaa taaaacgaaa gatagaagat gcgaagaata gtaaaaagct
     6961 tcttgaacaa aatgaacttt ctgataaaat aaaactaaaa gatttcttgg gaattcagaa
     7021 gttcgataca gagaagctgc gtgaggttat caaggtgatc cgtgttcata gtcaggatga
     7081 aattgaaatc gaatggaatt ttgacgatat tttttcagaa cagagataac ttgagcagga
     7141 caagaatgac ttatcaaaag ataataaagg tgctgggact acctgatgga agcgtcagat
     7201 agtcttcggc agaaaaaaat aaaatttttt ttgtcctata cttgacacat ccacatgtat
     7261 gcaggcacac cttttgctct aatatggcaa aatccgggat gaatccgaaa accttgcagt
     7321 atatcatggg tcacgcagac atcagtgtaa ccttgaacac ttacacccat gtgaattttg
     7381 atgatgcaaa ggaagaagta tatcggatag caaa
//
LOCUS       DBMDXG010000058         7394 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2606_length_7394_cov_1.889620, whole genome shotgun sequence.
ACCESSION   DBMDXG010000058 DBMDXG010000000
VERSION     DBMDXG010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_7394_cov_1.889620"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="ACZPD3_02915"
     CDS             complement(<1..1480)
                     /locus_tag="ACZPD3_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR1414990.1"
                     /translation="MAKAIMVQGTMSNAGKSLLAAGLCRIFKQDGYRVAPFKSQNMAL
                     NSFITEEGLEMGRAQVMQAEAAGIKPSVLMNPILLKPTNDVGSQVIVNGEVLGTMSAR
                     DYFKYKKKLVPDIMKAYDKLASENDIIVIEGAGSPAEINLKTEDIVNMGMAKMAKAPV
                     LLVGDIDRGGVFAQLIGTVELLEADEREMVKGLIINKFRGDKTILDPGVQMLEERSHI
                     PVVGVAPYLDIQVEDEDSLTERFDRKQEVDLIDIAVIRVPRISNFTDFNPLESIPGVS
                     LRYVQHVSELKNPDMIILPGTKNTMEDLLWMRANGLEAAVLKEAAKGKIIFGICGGYQ
                     MLGETLSDPHHVEAGGTIKGMGLLPMDTVFAEKKTRTRVSGRFLELEGELQALSGAEL
                     EGYEIHMGETVLKGEAGHSVSIEDQVSGECKEDGAYRKNVCGTYVHGVFDREDVAEAV
                     IRVLGEKKGIDVSQMTGIDFAAFKETQYDILAAELRKHLDMKK"
     gene            complement(1513..2556)
                     /locus_tag="ACZPD3_02920"
     CDS             complement(1513..2556)
                     /locus_tag="ACZPD3_02920"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1414991.1"
                     /translation="MTKHIHGGDVYKYDHCLDFSANCNPLGTPQSVKQAIIDSVEDLS
                     DYPRVGCGPLKEAIADYEYTKKEYLICGNGAADLIFSLSRALNPKKALLPAPTFAEYE
                     QALISVGCEISRYYLKEENDFCIQKDYPDVLKREKPDIIFLCNPNNPTGITIPQDLLE
                     EILETCAMQGIFMVVDECFLDFVKDPEKYTLKGKLEKYPGLFILKAFTKRYAIPGVRL
                     GYGFCSDRKVLDRMEAVTQPWNVSTMAQQAGMAALKESEYVEAGRQIIFRESAWMKEK
                     MRQVGLTVYPSEANYIFFYGPEDLFERCVAKGILIRDCSNYLGLKKGYYRVAVKLHEQ
                     NEKLIEVLEEVLS"
     gene            complement(2624..3601)
                     /gene="cbiB"
                     /locus_tag="ACZPD3_02925"
     CDS             complement(2624..3601)
                     /gene="cbiB"
                     /locus_tag="ACZPD3_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR1414992.1"
                     /translation="MLYYSMPALAAGFILDLMIGDPRWLYHPVCLIGNLIAFLEKILR
                     KIFPKTDKGELAAGIVEVILVCLLSGGIPFLILHILYGISVWAGFALETFWCYQLLAT
                     KSLKTESMKVYDRLKNGTLDEARYAVSMIVGRDTQSLTEEGVTKAAVETVAENASDGV
                     IAPMFYMAIGGVWLMFLYKGINTMDSMLGYKNDKYLYFGRCAAKLDDVANYIPARLSG
                     WLMVAASAFVKMDVKNAAKIYRRDRRNHASPNSAQTEAAMAGALEVQLAGNAYYFGKL
                     YEKPTIGDGIRPVEVEDIRRSNRLLYATAILGAVIFILIGSAVRYCFFL"
     gene            complement(3606..5000)
                     /locus_tag="ACZPD3_02930"
     CDS             complement(3606..5000)
                     /locus_tag="ACZPD3_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HIR1414993.1"
                     /translation="MDIPRILLAAGASGSGKTLITCGLLQALVNRKMKVASFKCGPDY
                     IDPMFHSRVIGTKSRNLDTFFTDAETTRYLLGKNAADCDIAVMEGVMGYYDGVGGIST
                     KASAYDLADTTDTPVILVVNSRGMSISLAAYIKGFMEYKEKSHIKGVIFNQMSPMLYP
                     RMKKLVEEQLEVEVLGYVPKVEDCVIESRHLGLVLPEEISDLRERLQKLAGILEDTLE
                     IDRILGLAKNAEELQVPESLIQKDRTYGYCLPQKLRIGVAKDEAFCFFYEDNFRLLQE
                     MGAELVDFSPIHDEHLPADLDGILLYGGYPELNGEALERNASMKEEIAQAVKQGMPCM
                     AECGGFMYLHEQMEDMGGVFRKTCGVIPGKCFRTPRLTRFGYITLTARKPVFGRSAEE
                     IGEIPAHEFHYFDSENCGSDFHAAKPLSKRGWDCIHSSSNLLAGYPHIYYYGNPQIPR
                     AFLMKCLEYHNKEF"
     gene            complement(5123..7174)
                     /gene="cobK"
                     /locus_tag="ACZPD3_02935"
     CDS             complement(5123..7174)
                     /gene="cobK"
                     /locus_tag="ACZPD3_02935"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HIR1414994.1"
                     /translation="MYKVLVFAGTTEGYEICRFLADHKIETKGFVATEYGSKSLTENE
                     FLTVQTGRLDAADMEEVFLQEKPEMVLDATHPYAAEVTVNIRTACENTQTAYYRVLRE
                     AGEHEDRAVYVDSVQAAADYLDQTQGSVLLTTGSKELAGFTGMKDYQNRLYARVLSLP
                     NVMKACAELGFEGKHLIGMQGPFSRELNAAMLRQYDCRYLVTKDTGKAGGFQDKIDAA
                     LECDAVPVIIGRPLKEEGMSVRECKRFLTEHFGLAHRPHITLLGIGMGSQKLLTVQGK
                     NSLDQADLLIGARRMVDSVKRPGQDVFVEYRSQEIRDYIDAHPEYDNIVIVLSGDVGF
                     YSGARKLLEVLCQDSADLRVQRKNGSEKSEEERDSSAQNNTEIEIQCGISSVVYFMSQ
                     IGLSWDDAKIVSAHGRGCNLISHICYAEKVFSILGTSDGVAVLAEKLVKYGMGDVLLY
                     VGENLSYENEKIFVKPASELTEYKGDPLSVICAVNENAGTRLETHGIRDEEFIRGKAP
                     MTKEEVRTVSLSKLRLTADSVCYDVGAGTGSLSIEMALRAHQGQVWAIEKKEDAVELI
                     HRNKVKFAADNLEIVEGLAPEALKDLPAPTHAFIGGSSGNLKEIVKLLLEKNPQVRIV
                     INCITLETVSEALETAKEFGFEENEIVQLSAARSKAIGRYHMMMGENPIYIITLQNPG
                     K"
     gene            complement(7167..>7394)
                     /locus_tag="ACZPD3_02940"
     CDS             complement(7167..>7394)
                     /locus_tag="ACZPD3_02940"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161256.1"
                     /note="catalyzes the formation of precorrin-4 from
                     precorrin-3B and S-adenosyl-L-methionine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR1414995.1"
                     /translation="DYLMRACDIMLKHKSPDTICGTVSNIAREGEEAHVMTLKELRDT
                     QVDMFTTVFIGNSQTKEINNKMVTPRGYRNV"
BASE COUNT         1809 a         2010 c         1355 g         2220 t
ORIGIN      
        1 ttttcttcat atccagatgc ttcctcagtt ctgctgccag gatatcatac tgggtttctt
       61 taaatgctgc aaaatcaatt cctgtcatct gagatacatc gattcccttt ttctcaccca
      121 gaacacgtat aacagcctct gccacatctt ccctgtcaaa aactccatgg acataggttc
      181 cgcagacatt tttacggtat gcaccgtctt ccttacactc tcctgacacc tgatcttcaa
      241 tggatacact gtgtccggcc tcgcctttta acacagtttc tcccatatgg atctcgtaac
      301 cctccagttc tgcgccggaa agtgcctgaa gctctccctc gagttccaga aatctgccgg
      361 aaactctggt tctggttttc ttttccgcaa aaacagtatc cataggaagc agtcccattc
      421 ccttgatggt tcctccggct tccacatgat gagggtcact taaggtctca cccagcatct
      481 gataaccgcc acagattcca aagatgatct taccctttgc agcttccttc aaaactgcag
      541 cttccagacc attagcccgc atccacagca gatcttccat tgtatttttt gttcctggaa
      601 ggatgatcat atccggattt ttcagttcac tgacatgctg tacatatctc aaggaaactc
      661 ccgggatact ttccagtgga ttaaaatctg tgaaattgga gatccttggc acccggatca
      721 ctgcaatatc aataagatcc acttcctgtt ttctgtcaaa tctttctgtg aggctgtcct
      781 catcctccac ctgaatatcc agatacggag ccactcccac tacagggata tggcttctct
      841 cctccagcat ctgcactccc ggatcaagga tggtcttgtc gccgcggaat ttgttgataa
      901 tcagaccctt taccatctct ctttcatccg cttccagaag ctccactgta ccgatcaact
      961 gtgcaaaaac accacctctg tcaatatctc ccaccagaag caccggtgcc tttgccatct
     1021 ttgccattcc catatttacg atatcttctg ttttcagatt gatctccgcc gggcttccgg
     1081 ccccctcgat cacaatgata tcattttctg atgccagttt atcataggcc ttcataatgt
     1141 caggcaccag ctttttctta tatttaaaat aatctctggc actcatggtt cccaggactt
     1201 ctccgtttac gatcacctgg gagcctacat cattggtagg cttcagaaga atggggttca
     1261 tcagcacaga aggcttaatt ccggctgctt ccgcctgcat cacctgagct ctgcccatct
     1321 ccagcccctc ttctgtaata aaagagttca gtgccatatt ctgggattta aagggtgcca
     1381 ccctgtatcc atcctgttta aaaatcctgc aaagccctgc tgccagaagg ctttttcctg
     1441 cattggacat tgtcccctgc accataatcg cttttgccat tttttgtttc cccttttctc
     1501 ctgtataaat acttatgaaa gcacttcttc cagtacctct atcagctttt cattctgttc
     1561 atgcagcttc actgccacac ggtaatatcc ttttttcagc cccagatagt tactgcaatc
     1621 ccggatcagg atcccctttg caacacatct ttcaaagaga tcctcaggtc cgtaaaagaa
     1681 tatataattt gcttcagaag gatataccgt cagcccaacc tgtctcatct tttccttcat
     1741 ccatgcagat tccctgaaaa tgatctgccg tcctgcttcc acatattctg attccttcag
     1801 tgctgccatt cctgcctgct gtgccatggt agacacattc cagggctggg tgactgcctc
     1861 catccggtca agtactttcc tgtccgaaca gaatccatag cccagacgca caccggggat
     1921 cgcatatctt ttggtaaatg ccttaagaat aaaaagtccc ggatattttt ccagttttcc
     1981 cttcagcgta tatttctccg gatcttttac aaaatccagg aagcactcat ctactaccat
     2041 aaatattccc tgcatggcac aggtttccag gatttcttcc agaaggtcct gaggtatggt
     2101 aatccctgta ggattattgg gattacacag gaaaataata tccggctttt ctcttttcag
     2161 aacatccgga taatctttct gtatacagaa atcattttcc tctttcagat aatatctgct
     2221 gatttcgcag ccaacactga tcagtgcctg ttcatactcc gcaaaggtag gagcaggaag
     2281 aagggccttc tttggattca atgccctgct gagggaaaaa ataaggtctg cggctccatt
     2341 cccgcagatc agatattctt tttttgtata ttcataatct gcgattgcct ctttcagagg
     2401 tccgcatcct acacggggat aatcactcag gtcctctacg ctgtctatga tcgcctgctt
     2461 tacactctgc ggtgttccca acggattaca gtttgcagag aaatccagac agtgatcata
     2521 tttataaaca tcaccgccat gtatatgttt tgtcatcagt tgtctcctgt cttactatac
     2581 atgttttata ttaattacta ttcggcaatc cacgttaccg gaattataaa aagaaacaat
     2641 atcgcaccgc gcttccgatc agaataaata tcacagcccc caggatcgct gttgcataaa
     2701 gcagacgatt ggaacgtctg atatcctcca cctccacagg gcggattcca tctcctatgg
     2761 ttggtttctc ataaagcttt ccgaaatagt aagcattgcc tgcaagctgt acctccagcg
     2821 cacctgccat tgctgcctct gtctgtgcag agttggggct ggcgtgattt cttctgtctc
     2881 tgcggtaaat cttcgctgca tttttcacat ccatttttac aaatgcagag gctgccacca
     2941 tcagccatcc tgaaagtctg gctggaatat aattggccac atcatccagc tttgcagcgc
     3001 aacgaccaaa atacagatat ttatcattct tataacccag catggaatcc atggtattga
     3061 ttcctttgta aagaaacatc agccacactc cgccgattgc catatagaac atcggtgcta
     3121 tgacaccgtc tgaagcattc tctgccacag tctctactgc cgcctttgta acaccctcct
     3181 ctgtcaatga ctgggtatca cggcctacga tcatggatac tgcataacgg gcctcatcca
     3241 aagttccgtt tttcagtctg tcatagactt tcatgctctc tgttttcaga gatttcgttg
     3301 ccagaagctg ataacaccag aaggtttcca gtgcaaatcc cgcccatact gagattccat
     3361 agagaatgtg gaggatcaga aatgggattc caccgcttaa cagacagaca aggatcactt
     3421 ccactatacc tgcagccagt tcgcccttat ctgttttcgg aaaaatcttc cgaagtattt
     3481 tttccagaaa tgcgatcaga tttccgatca gacacaccgg atgatacagc catctgggat
     3541 ctcctatcat aagatcaagt atgaagcctg ctgcaagggc aggcatactg taatataaca
     3601 tatttttaaa actccttgtt atgatattcc agacatttca tgagaaaagc cctgggaatc
     3661 tggggatttc cataataata gatatgagga tatcctgcca gcagattact gctgctatgt
     3721 atacagtccc agcctctttt acttaaaggc ttcgccgcat ggaagtcact tccacagttc
     3781 tcagaatcaa aatagtggaa ttcatgggcc ggaatctcgc cgatctcctc tgcacttctg
     3841 ccaaaaacag gtttcctggc ggttaatgtg atatatccaa atctggtcag tctgggagta
     3901 cggaaacatt ttccggggat cactccgcag gttttccgga acactccgcc catatcctcc
     3961 atctgctcat gaagatacat gaagcctccg cattctgcca tacatggcat gccctgttta
     4021 actgcctgtg caatctcttc tttcatggat gcattgcgtt ccagtgcctc tccatttaat
     4081 tccggataac cgccataaag aagaatccca tccagatctg caggaagatg ttcatcatga
     4141 attggcgaga aatcaaccag ctctgccccc atttcctgaa ggagacggaa attgtcttca
     4201 tagaagaaac aaaaggcttc atcctttgcc actccgatac gcagcttctg tggcagacaa
     4261 tatccatatg tcctgtcttt ctgtatcaga gattccggca cttgcagctc ctctgcattt
     4321 ttggcaagcc caaggatcct gtcgatttcc agtgtatctt ccaatattcc ggccagcttc
     4381 tgcaagcgtt ccctcagatc agaaatctcc tcaggaagca caagacccag atgacggctc
     4441 tcgatcacac agtcctctac ctttggcaca tatccaagaa cctccacctc aagctgttct
     4501 tccaccagct tcttcattct gggataaagc atgggagaca tctggttaaa gatcacgcct
     4561 ttgatatggc ttttctcttt gtactccata aaacctttga tataagctgc cagggaaata
     4621 ctcattcctc tgctgtttac cacaaggatg accggagtat ctgtggtatc tgccaggtca
     4681 taggcgcttg ctttcgtgga tattccaccc acgccgtcat aatagcccat aacaccttcc
     4741 atcactgcaa tatcgcagtc tgctgcattt tttccaagca ggtatcgggt agtctcggca
     4801 tctgtaaaaa aggtgtccag attacgggat ttcgtcccga tgactctgga atgaaacatg
     4861 ggatctatat aatccggtcc gcatttaaaa gatgccactt tcatttttcg attgaccagt
     4921 gcctgcagaa gtccgcaggt gatcagtgtt ttgccactgc cgctggctcc tgctgcaaga
     4981 agtattcttg gaatatccat ccctgttctc ctctgtttct gtcctatatt ttttcaacaa
     5041 tttcagggca ttccaatcta ttaactgtta atctgtattt ccttaactgt taatttgtaa
     5101 tccctgatat ctgcatcatt tgttatttcc cgggattctg aagtgtaata atataaatgg
     5161 gattctcgcc catcatcata tgataacgtc cgattgcctt tgaacgtgct gcacttagct
     5221 gtacgatctc attctcttca aatccaaact ctttcgctgt ttccaatgct tcagacacag
     5281 tttcaagagt gatacagttg atcacgatcc ggacctgagg gttcttttca agcaacagct
     5341 tcacgatctc tttcagattt cctgacgagc ctccgataaa agcatgggtt ggtgccggaa
     5401 gatctttcag agcctccggt gcaagtcctt ccacaatctc cagattatcc gctgcaaatt
     5461 tcaccttatt ccggtggatc agttcaactg cgtcttcctt tttctcaatt gcccagacct
     5521 gtccctgatg agcacgaagt gccatctcta ttgacagtga gcctgttcct gcacccacat
     5581 catagcagac agaatcagca gtcagccgca gtttggaaag ggataccgta cgcacctctt
     5641 cctttgtcat gggcgctttg cctctgataa attcctcatc tctgatccca tgcgtctcca
     5701 gacgtgttcc tgcattttca tttactgcac agatgacgga caaaggatca cccttatatt
     5761 ctgtcagttc actggctggt tttacaaaaa tcttctcatt ttcataagac agattttctc
     5821 ccacataaag gagcacatca cccatcccgt attttaccag cttctctgcc agaactgcta
     5881 caccgtctga agttccaagt atggaaaaga ctttttctgc ataacagata tgtgagataa
     5941 gattacagcc tcttccatgt gcactgacga ttttggcatc atcccaggac aatcctattt
     6001 gggacataaa atatacaact gaggaaatcc cacactgaat ctctatctct gtattatttt
     6061 gtgctgaact atcccgttct tcctcactct tctctgatcc gttctttctc tgtactctca
     6121 gatccgcact gtcctgacac agtacttcca gaagctttct ggctccgctg tagaagccca
     6181 catctccgga aaggacgatc acaatattgt catactccgg gtgtgcatca atataatccc
     6241 tgatctcctg gcttctgtat tccacgaaca catcctgtcc cggacgtttc acagaatcca
     6301 ccatcctcct ggcaccgatc agaagatccg cctgatccag gctgttctta ccctggacag
     6361 tgagaagttt ttgacttccc attccaattc ccagcaatgt aatatgcggt ctgtgggcta
     6421 aaccgaaatg ctctgtaaga aatctcttac attctctcac agacatgcct tcctccttca
     6481 gaggacgccc gatgatcaca ggaactgcat cacattccag ggcagcatcg atcttatcct
     6541 gaaatccccc tgcttttcct gtatcctttg tgaccagata tctgcagtca tactgtctga
     6601 gcattgcagc attcagctct ctggaaaaag gcccctgcat tcctatcaga tgtttcccct
     6661 caaatcccag ctctgcacag gctttcatca cattgggcag agaaaggact cttgcataaa
     6721 gacggttctg ataatccttc atgcctgtga agccggccag ttccttgctt cctgttgtca
     6781 gcagtacact tccctgtgtc tgatccagat aatctgctgc agcctgaaca ctgtccacat
     6841 atactgccct gtcctcatgt tcccctgctt ctctcagcac cctgtaatat gctgtctgcg
     6901 tattttcaca ggctgtcctg atatttacag tcacctcagc agcataagga tgggtggcat
     6961 ccagcaccat ttccggtttc tcctgtaaaa agacctcttc catgtccgct gcatccagtc
     7021 ttccggtctg aacagtgaga aattcatttt ctgtaagaga tttgctgcca tattctgtgg
     7081 caacaaatcc ctttgtctct atcttatgat ctgccagaaa ccggcagatt tcgtatcctt
     7141 ctgttgttcc cgcaaaaaca agaactttat acattcctat atcccctcgg tgttaccatc
     7201 ttattgttga tttcctttgt ctgagaatta ccgataaata ctgtggtaaa catatccacc
     7261 tgtgtatctc tcagttcctt taatgtcatt acatgagctt cttcgccttc tcttgcaata
     7321 ttggaaactg ttccgcagat ggtatccggt gatttgtgtt tcagcattat gtcacacgcg
     7381 cgcattaaat aatc
//
LOCUS       DBMDXG010000059         7318 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2638_length_7318_cov_1.764999, whole genome shotgun sequence.
ACCESSION   DBMDXG010000059 DBMDXG010000000
VERSION     DBMDXG010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_7318_cov_1.764999"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..1066
                     /gene="pstC"
                     /locus_tag="ACZPD3_02945"
     CDS             209..1066
                     /gene="pstC"
                     /locus_tag="ACZPD3_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR1414996.1"
                     /translation="MNHFKEKAMKCVFLIAACTSVLAVFLICAFLFANGVPAIGKIGP
                     LKFLLGTKWKPSNDIFGILPMIVASIYVTAGAILLGVPIALFTSVFMARYCPKKIYRP
                     LKSGIELMAGVPSIVYGFFGLILIVPLIRQIFGGTGTSMLAACVLLGMMILPTIIGVT
                     ESAIRSVPESYYEGSLALGATKERSIFFVMLPAAKSGILAAVVLGIGRAIGETMAVVM
                     VAGNQPRMPQGILKGVRTMTANIVTEMGYATGLHREALIATAVVLFIFILIINLSLSL
                     LNRRAEHAN"
     gene            1056..1961
                     /gene="pstA"
                     /locus_tag="ACZPD3_02950"
     CDS             1056..1961
                     /gene="pstA"
                     /locus_tag="ACZPD3_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR1414997.1"
                     /translation="MQIDTAAAIDLSKEKKQNESSFSDQMKAYIKHPGSGVLALLTLL
                     GAVLTFALLFFLIGYILVKGVPYLSTDLFSLTYNSENLSLLPSLINTFILTVVSLVIA
                     APLGIFAAIYLVEYAKKGSKLVNVIRITAETLSGIPSIVYGLFGMLFFVTALHWGLSL
                     LSGAFTLVIMILPLIMRTAEEALKSVPDSYREASFGLGAGKLRTIFTIVLPSAVPGIL
                     AGVILAIGRVIGETAALIYTAGTVAEVPKNLMGSGRTLALHMYVLSGEGLHMNQASAT
                     AVVILAFVLVINFLSGAVAKRIAKG"
     gene            1967..2725
                     /gene="pstB"
                     /locus_tag="ACZPD3_02955"
     CDS             1967..2725
                     /gene="pstB"
                     /locus_tag="ACZPD3_02955"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR1414998.1"
                     /translation="MSGNEKITVENMNLHYGKFHALRNINMAIPEKEITAFIGPSGCG
                     KSTLLKSLNRMNDLVEGCVITGKVQLDGEDIYGDMDVNLLRKRVGMVFQKPNPFPMSI
                     YDNIAYGPRTHGIRSKSKLDDIVEKSLRDAAIWDEVKDRLKKSALGMSGGQQQRLCIA
                     RALAVQPEVLLMDEPTSALDPISTSKIEELAMELKKDYTIVMVTHNMQQATRISDKTA
                     FFLLGEVVEFDDTDKLFSMPSDKRTEDYITGRFG"
     gene            2728..3399
                     /gene="phoU"
                     /locus_tag="ACZPD3_02960"
     CDS             2728..3399
                     /gene="phoU"
                     /locus_tag="ACZPD3_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR1414999.1"
                     /translation="MSKYFERQLEELHVQLITLGSYCEKAISFSAKAIQKVQNEEIAR
                     QVFETDRDIDAKEREIENLCLSLLLHQHPVARDLREISAALKMVSDMERIGDQAADIA
                     DLSLYVAKNTTAIPETITQMAEDTVRMVTESVDAFVKSDLELCRNVIDRDDVVDDAFN
                     EIKEKLADMIYGGNLDAKTGLDLLMTAKYFERIGDHAVNIAEWVEYSITGQHRNNEHQ
                     DYLNN"
     gene            3560..5227
                     /locus_tag="ACZPD3_02965"
     CDS             3560..5227
                     /locus_tag="ACZPD3_02965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415000.1"
                     /translation="MTKKIFKSIMFVCALVLTVGLAAVMGILYSNFDGQMRKELSKEA
                     AYLAYGVEQQGTDYLKNVKDKSSRITYINKDGTVLFDNKADADEMQNHKNRTEFQKAE
                     KYGAGECSRYSDTLSEKTIYYALRLKDGTVLRVSGTQDSVLALVENLLLPLCGLLFLM
                     LILSGIMASVISKRIVKPVNELDLEHPEENKIYEELSPLLGKIHKQNRQIQKQLELAK
                     QQQEEFSLITENMQEGLIVIDRYTMILSANSSAWNLFRVDKVCQGESVYCLDRAEDFR
                     HAIEQVLSGEHAELILKLNGSDIQLIANPVVRGQKTEGAVILLVNVTEKLERENLRRE
                     FSANVSHELKTPLTSISGFAEIMQGGLVKCEDIPKFAGRIYKESQRLLQLVEDVIQIS
                     QLDEEKTSYTWELVDVYQVCKNAFESLKEKAQSMNVHLYICGDSMKMEAVRTLLEEAV
                     YNVCDNAIKYNRNDGSVSIFLTQTAHEIQIVVKDTGVGIPKEDQDRVFERFYRVDKSH
                     SKEIGGTGLGLSIVKHAVSTLNGSIVLRSEEGSGTEITMKFLKVHKE"
     gene            complement(5296..6879)
                     /locus_tag="ACZPD3_02970"
     CDS             complement(5296..6879)
                     /locus_tag="ACZPD3_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1415001.1"
                     /translation="MNALLVDDDRFVVAALEKKINWEQLTITEVLTAFNIRQAQKIIE
                     KNSIDICVCDIEMPGGSGLDLLSWVRESGKEIQFIFLTSYADFDYAKKAIELSSLDYQ
                     LKPIDFDTLFHILEKAVSKVRKNAALTQTKADSQKWKDNYRYIVDLFWKELFATTLFR
                     EPSLLETELRKKDLSYTADDLFIPVLFRLYPLSGQIMPMESSMVDFSFQNITAETFQK
                     SCILYESIVILNTFEYVVILSGLQLEQIRQPFTENLLTLFKNLQSFLHCEIACGIAPE
                     VSLTELPETLTRLREMRESNLNSVNKPLFLQDFVPSTTSYIPPSLEVINTFLEQKQAD
                     AALQNIENYLSKYIQASGITKNFLLHLRLDIEQIVFSYLHKNGIEAHTLFSSEERDEL
                     ITKSLDAVPYMFNYLRYLIQRAVEYNSFINEKDSVVDIVLNYIHQHYSEDISRTMLAD
                     MVYLNPDYLARLFKKQTQTSIINYITTYRLEKAKELLLNPDIPVGTVALKVGYGNYSY
                     FSKLFKDVVGCTPNEYRKK"
     gene            complement(6916..7167)
                     /locus_tag="ACZPD3_02975"
     CDS             complement(6916..7167)
                     /locus_tag="ACZPD3_02975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415002.1"
                     /translation="MTAGGNSHENIQIQVMDTGCGFSEDILQSLNSAIPLEPQNGHRI
                     GITNAIQRLNLLYGQGEAVITFSNLPSGGACVTILLPAI"
BASE COUNT         2131 a         1279 c         1803 g         2105 t
ORIGIN      
        1 gcgatcaggt aaaggctgta tacacaggcg agactactac atgggaagat cttgctaaat
       61 aatgtaacag atttagtttc agagcagttc gcctgtgaat gaaatagtac agcaggcggg
      121 ctgtttgcat gttgaaaatc atattttcag aatcactgta aaagtgacag gttatgattt
      181 ttgatacagg gaaagaaagg tgtaatgaat gaaccacttt aaagaaaaag caatgaaatg
      241 tgtatttctg atcgcagcct gtacttcagt gttggcagtg tttctgatct gtgcatttct
      301 gtttgcaaat ggtgtcccgg ctatcgggaa aatcggtccg ctgaagttcc tgctgggaac
      361 aaagtggaag ccgtcaaatg atatttttgg aatccttcca atgatcgtag caagtatcta
      421 tgtaactgca ggtgccattc tcctgggtgt gccgattgca ctctttactt ctgtttttat
      481 ggcacgttac tgcccgaaga aaatttacag accattgaaa tcaggaattg aattgatggc
      541 aggtgtacct tctattgtat atggtttttt tggcctgatt ctcattgttc ctctgatcag
      601 acagattttc ggcggaacag gaaccagtat gctggcagcc tgtgttctgc ttggaatgat
      661 gatcctgccg actatcatcg gagttacgga atctgcgatc cgcagcgtac ctgagagtta
      721 ttacgaaggt tcactggcac tgggagcaac caaggagaga agcatctttt ttgtcatgct
      781 tccggcagca aaatcaggta ttctggcagc agtagtactt ggaatcggtc gtgcgatcgg
      841 tgagacaatg gcagttgtaa tggttgcagg taaccagccg agaatgccac agggaatcct
      901 gaaaggtgtc cgtacaatga ctgcaaatat cgtcactgag atgggttatg caacaggtct
      961 tcacagagaa gctctgatcg caacagcagt tgtgttattt atctttattc ttatcattaa
     1021 cctgagtctt tctttactga acaggagggc agaacatgca aattgataca gcagcggcaa
     1081 ttgatttgtc caaagaaaaa aagcagaatg agtcttcttt ctcagatcag atgaaggcat
     1141 atattaagca tccgggatca ggtgttcttg cattgcttac ccttttaggt gcagtcctga
     1201 catttgcatt actgttcttt ctgatcggat atattctggt caagggcgta ccgtatctgt
     1261 caacagactt gttcagttta acttataatt cagagaatct ttccttactt ccttctctga
     1321 tcaacacatt catcctgacg gttgtttcac ttgtaatcgc agcaccgctg ggaatctttg
     1381 cagccatcta tctggtagaa tatgcgaaaa aaggaagtaa gcttgtaaat gtgatccgta
     1441 ttacagcaga aacactttca ggaattcctt ctattgtata tggccttttc ggtatgctgt
     1501 ttttcgtaac agcactgcat tggggacttt ctcttctgtc cggtgctttc acattggtaa
     1561 ttatgattct tcctctgatc atgcgtactg cggaagaagc cctgaaatct gttcctgatt
     1621 cttatcgtga agccagcttt ggacttggag caggtaaact tcgtaccatc ttcaccattg
     1681 ttctgccatc agcagtacct ggtattctgg caggtgtgat cctggccatc ggacgtgtga
     1741 tcggcgaaac agcagccctg atttatacag caggtacagt tgcagaggta ccgaagaact
     1801 tgatgggttc cggacgtact ctggcacttc atatgtatgt gctgtcaggt gagggccttc
     1861 atatgaatca ggcatctgca acagcagttg ttattctggc atttgttctt gtaatcaatt
     1921 tcctgtccgg agcagttgcc aaacgaattg cgaaaggata agaaaaatga gtggaaatga
     1981 aaaaataaca gttgaaaata tgaacttaca ttacggaaag tttcatgcgc tgaggaatat
     2041 aaacatggca attccggaga aagaaatcac agcttttatc gggccgagtg gctgtggtaa
     2101 atctacactt ctgaaatctt taaaccgtat gaatgacctt gttgagggat gtgtgatcac
     2161 cggtaaggta cagcttgacg gtgaagatat ttacggagat atggatgtga atcttctgag
     2221 aaaaagagtg ggaatggtat tccagaagcc taatcctttc cctatgagta tttatgataa
     2281 cattgcatat ggaccgagaa cccatggaat ccgttccaag tctaaactgg atgatattgt
     2341 agaaaaatct ctccgcgatg cggcaatctg ggatgaagta aaagacagat taaagaaaag
     2401 cgcactgggt atgtcaggcg gccagcagca gagactttgc attgccagag cactggcagt
     2461 tcagccggag gtacttctga tggatgagcc tacatccgca cttgacccta tctcaacttc
     2521 caagatcgag gagctggcga tggagttaaa gaaggattat accattgtta tggtaactca
     2581 taatatgcag caggctacac gtatttctga taaaacagct ttcttccttc tgggagaagt
     2641 agttgaattt gatgatactg ataaactgtt ctctatgcct tctgacaagc gtacagagga
     2701 ttacattaca ggaaggtttg gttgattatg tcaaagtatt tcgaaagaca gctggaggaa
     2761 ctgcacgttc agcttatcac actgggaagc tattgtgaga aagcaatttc attctctgca
     2821 aaagcaatcc agaaggtgca gaatgaagag attgcccgcc aggtttttga gacggacaga
     2881 gatattgatg ccaaagaaag agagattgag aatctgtgcc tgagtcttct gcttcatcag
     2941 cacccggttg caagagattt aagagagata tctgcagcat tgaagatggt ttctgatatg
     3001 gaacgtatcg gtgaccaggc agcagatatt gcagatctgt ctttgtatgt tgcaaagaat
     3061 actactgcga ttccggagac tatcacacag atggcggagg acactgtgcg tatggtaact
     3121 gagagtgtgg atgcttttgt aaagagtgat ctggagttgt gcagaaatgt cattgacaga
     3181 gacgatgtgg tggatgatgc atttaatgag atcaaagaga agcttgcaga tatgatttac
     3241 ggaggaaatc tggatgccaa gacagggctg gatcttctta tgactgcaaa gtactttgaa
     3301 cgtatcggtg atcacgcagt gaatattgct gaatgggtgg aatattctat taccggacag
     3361 cacagaaata atgagcatca ggattatctg aataattaat tgaatagaga aagtctctga
     3421 ttttgaatat aattatttgg aagcagggct ttttatatcc gcaggcggga accgtttgcg
     3481 gatatttgag ttattatata tctcaatcgg agaccgatat ctgcctgagg tattaaataa
     3541 aaaggggaag tgaagagaag tgacaaaaaa aatctttaaa tctataatgt ttgtatgtgc
     3601 attggttctg actgtgggac tggctgctgt aatgggaatc ctttacagca attttgacgg
     3661 acagatgcgc aaagagctgt ctaaggaagc tgcttatctg gcatatggtg tggagcaaca
     3721 gggaacagat tatctgaaga atgtaaagga taagagctca aggattactt atattaataa
     3781 ggatggaact gtgctttttg ataataaggc tgatgcagat gaaatgcaaa atcataagaa
     3841 cagaactgaa tttcagaaag cagagaaata tggtgcagga gaatgttcca ggtattcgga
     3901 tacactttct gagaaaacaa tttattatgc actgcgtctg aaggatggaa ctgtgctgag
     3961 agtttcaggc actcaggatt ctgtgctggc actggttgaa aatcttcttc ttcccttatg
     4021 tggactctta tttctgatgc tgattctatc aggaatcatg gcatctgtga tatcaaaaag
     4081 gatcgtaaaa cctgtgaatg agctggatct ggagcatccg gaggagaata agatttacga
     4141 ggagctttct ccgcttttag gtaaaatcca taaacagaac cggcaaatcc agaagcagct
     4201 ggaactggca aaacagcagc aggaagaatt ctctcttatt acagaaaata tgcaggaagg
     4261 tctgatcgtg attgataggt ataccatgat tctttctgcc aattccagtg catggaatct
     4321 gttccgggta gataaagtat gtcagggaga aagtgtttac tgtctggatc gtgcggaaga
     4381 tttcagacat gcgatcgagc aggttctcag cggagaacat gcggaactga tattgaaatt
     4441 aaatggaagc gatatccagt tgattgccaa tcctgtggta cgtggacaaa aaacagaagg
     4501 tgctgtgatc ctgcttgtga atgtgacaga gaagcttgag agggagaatc tgcggagaga
     4561 gttttcggca aatgtctctc atgagctgaa gactcctctg acttctattt caggatttgc
     4621 ggagatcatg cagggcggtc tggtgaaatg tgaggatatt cccaagtttg cagggcgtat
     4681 ttataaggaa tcccagagac tgcttcagct ggttgaggat gtgatccaga tttcccagct
     4741 tgacgaggaa aaaacatcct acacctggga actggtggat gtttatcagg tttgtaagaa
     4801 tgcctttgaa agtctgaagg agaaagcaca gagcatgaat gtgcatcttt atatctgtgg
     4861 ggacagtatg aagatggaag cagttcggac acttctggaa gaggcagttt ataatgtctg
     4921 cgataatgcc attaagtata accgaaatga tggaagtgta agcatttttc tgactcagac
     4981 tgcacatgag atacagattg tagtcaagga tacaggtgtg ggaattccaa aggaagatca
     5041 ggatagagtt tttgagagat tttacagagt ggataaaagt cattccaaag agattggagg
     5101 taccggactg ggactttcta ttgtgaagca tgcggtcagc actttgaatg gaagtattgt
     5161 gctgagaagt gaagaaggaa gtggaacgga aattactatg aaatttctga aagtgcataa
     5221 ggaataaaat agaatagata cagaggggat gtcagtttga cagcccctct aaattatacc
     5281 gtagaaagaa cctttttatt tttttcgata ttcattgggg gtgcagccga ccacatcttt
     5341 aaacagtttg gaaaagtagg aataatttcc atagccgact ttcaaagcaa ctgttccaac
     5401 cgggatgtca gggttgagca gaagctcttt tgctttttct aaccgatagg tggtgatgta
     5461 attgatgata gaggtctgcg tttgtttttt gaaaagtcgt gccagataat ctggattgag
     5521 ataaaccata tcagccagca tggtacgtga gatatcttcg gagtagtgct gatggatata
     5581 gttgagtaca atatcaacga cggagtcttt ttcgttgatg aagctattgt attctacggc
     5641 gcgttgtatc agatagcgca gataattaaa catatatggt acagcgtcca gagattttgt
     5701 gatcagttcg tctctttctt ctgaagaaaa tagagtgtgt gcttcgattc cgtttttatg
     5761 tagataggaa aaaacaattt gttcgatatc aagtctcaga tgcagaagaa aatttttggt
     5821 gataccactg gcctgaatgt atttggacag gtaattttcg atattctgta aagctgcgtc
     5881 tgcctgtttt tgttccagaa aagtattgat tacttccagt gatggaggga tgtaggaagt
     5941 tgtgctggga acaaaatctt gcagaaaaag tggtttattt acggagttaa ggttggattc
     6001 tctcatttca cgtagtctgg taagagtttc aggcagttct gtcagggaaa cctctggtgc
     6061 aatgccgcat gcaatttcac agtgcagaaa actctgaaga tttttgaaca gtgtcagtag
     6121 attttctgta aatggctgac ggatttgttc cagttgcaat ccagaaagta tgacaacgta
     6181 ttcaaaggtg ttcagtatca ctatagactc atagagaatg caggatttct gaaaggtttc
     6241 ggctgtaata ttctggaaag aaaaatctac catggaagac tccatgggca ttatctggcc
     6301 ggagagtgga taaagccgga acaaaacagg aatgaagagg tcatctgctg tataggaaag
     6361 atcttttttt cttaattctg tttcaagaag agaaggttca cgaaacagag tggtggcaaa
     6421 aagttctttc cagaataaat ctacaatata gcggtaatta tctttccatt tctgactgtc
     6481 tgcttttgtc tgggtgagcg cagcattctt tctgacttta gaaactgctt tttccagaat
     6541 atggaataaa gtatcaaagt ctataggttt tagctggtaa tcgagggagg acagttcgat
     6601 agcttttttt gcgtaatcaa aatccgcata gctggtcagg aaaataaatt gtatttcttt
     6661 tcctgattcc cggacccagg aaagaagatc aagaccagaa ccgccgggca tttcgatatc
     6721 gcaaacacag atatcgatgg agtttttttc tatgattttc tgagcttgac ggatattgaa
     6781 agcagtgagt acttctgtga ttgttagttg ctcccagttt atttttttct caagggccgc
     6841 tacaacaaag cggtcatcat caactaataa tgcgttcata aaactttcct ttcataaatt
     6901 aggcagagaa ttggatcaga ttgcagggag aaggatggtt acgcaggcac ccccagacgg
     6961 gaggtttgag aaagtaatga ccgcttctcc ctgaccgtac agcagattca gacgttggat
     7021 ggcattggta attccaatac ggtgaccatt ttgaggttcc agaggaatag cagaatttag
     7081 ggattgcagg atgtcttccg agaatccgca tccagtgtcc atgacctgaa tctgaatatt
     7141 ttcatgtgaa tttccgccgg cagtcatcca ggcgatgtct gtgcgtatga ttatgggttc
     7201 aacaaagaga aaacacccgc acacacaacc aagacaaacg acatcgcatt atgaaccccg
     7261 aaaaggccgg aaattcaggg ttcataatgc gatgtcgttt gtcttggttg tgtgtgcg
//
LOCUS       DBMDXG010000060         7204 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2685_length_7204_cov_2.253836, whole genome shotgun sequence.
ACCESSION   DBMDXG010000060 DBMDXG010000000
VERSION     DBMDXG010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2685_length_7204_cov_2.253836"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="ACZPD3_02980"
     CDS             <1..435
                     /locus_tag="ACZPD3_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415003.1"
                     /translation="IAYTRIGITVAMIFVGIPFVVRSVQPVLEKMDNSYSEAAGVLGA
                     KKTTIFWKVIFPELKPAIFAGTGLAFGRCLGEYGSVVFIAGNKPYYTEITPLVIMSEL
                     QEFDYSSATAIALVMLAVSFFILFLNSMIQQRNARILRGGNA"
     gene            435..1259
                     /gene="cysW"
                     /locus_tag="ACZPD3_02985"
     CDS             435..1259
                     /gene="cysW"
                     /locus_tag="ACZPD3_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HIR1415004.1"
                     /translation="MDNNEKTSEKITKWLLVGISVLFLAVMLLLPLITVITEALRSGW
                     KVYASAVTDEYTIKALLLTVKATLFAVGFNTIFGIFAAWALTKFQFRGKKFLTTLIDL
                     PVTISPVIAGLIFLLIFGRQSPVYFFLMKLGIKVVFSVPGIIIATVFVTFPFISRELI
                     PVLEAEGSDEEEAAALMGAGGWTIFWKITFPHIKWALLYGVVLCTARAMGEFGAVSVL
                     SGHLRGKTNTLPLHVEILFNEFQYVPAFAVSSLLVIMAVIILVVRSLIEYKGKKEA"
     gene            1261..2319
                     /locus_tag="ACZPD3_02990"
     CDS             1261..2319
                     /locus_tag="ACZPD3_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR1415005.1"
                     /translation="MYVELKNINKTYGSYQASRNVNLGIEKGKLIGLLGPSGSGKTTI
                     LRMIAGLETPDSGEVIIDGKVVNDVPASQRGIGFVFQNYALFRYMTVYDNVAFGLKVQ
                     KADKKKIDARVRELIKLVGLEGLEKRYPSQLSGGQRQRVAFARALAPNPQVLLLDEPF
                     AAIDAKIRQELRSWLKEMIGKLGITSIFVTHDQDEAIEVADEIIITNAGRIEQKGTPI
                     GVYRNPETAFTASFFGQPSVLKNADDFHTFAQAEGADKIIVRPEFVKISKLDEVEKFK
                     TSVSRGVVERVSFRGDNLELQVRVNNSVVTARRSLEKADIREGETVNVFLYRIFALRG
                     DSVQLIENEAVRDDFVII"
     gene            2431..4176
                     /locus_tag="ACZPD3_02995"
     CDS             2431..4176
                     /locus_tag="ACZPD3_02995"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HIR1415006.1"
                     /translation="MRTERIRTDVLIIGGGTAGCFAAYVIGQKSGRKVLIAEKANIKR
                     SGCLAAGVNALNAYITEGRVPQDYVDYAKKDAHGIVREDLLLTMSERLNHVTKVMEDL
                     GLVILKDENGKYVARGNRNIKINGENMKPILAKAAAEQENVTVLNHVNITDYKTENKK
                     VTGAVGFSVNEDIFYDIDAKAVLCATGGAAGLYRPNNPGFSRHKMWYPPFNTGAGYAM
                     GLLSGAEMTTFEMRFIALRCKDTIAPTGTIAQGVGARQVNAHGDIYETKYGLTTSQRV
                     YGTVMENREGNGPCYLRTEGISKEQEQDLYKAYLNMAPSQTLKWMEAGKGPSEENVEI
                     EGTEPYIVGGHTASGYWVDNNRATTIEGLYAAGDVAGGCPQKYVTGALAEGEIAAEAI
                     LEYLNGKDDNRIAAENKEGALESGGEQELSQTTIAKKAEYESHLNASRSLMNAEQLEE
                     AMQKIMDQYAGGIGTDYRYSGMSLAKADEKIAALLPVVDTLAATDTYELMQIYELRER
                     LIVCQAVIAHLAARKETRWHSFGENTDYPQQSEEWMKYVNSRMENGEIKILYRDLITK
                     ENTGLNTAEKGAGER"
     gene            4173..4487
                     /locus_tag="ACZPD3_03000"
     CDS             4173..4487
                     /locus_tag="ACZPD3_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1415007.1"
                     /translation="MSIRIQKSKCAGCGRCIEACPGNLIKKDKENKAFIRQVRDCWGC
                     TSCIKECRHDAIRFFLGADVGGRGASMVVSEKPDISTWTVEKPDGTKITIQVNKKDAN
                     KY"
     gene            4545..4631
                     /locus_tag="ACZPD3_03005"
                     /pseudo
     CDS             4545..4631
                     /locus_tag="ACZPD3_03005"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            complement(4599..4718)
                     /locus_tag="ACZPD3_03010"
                     /pseudo
     CDS             complement(4599..4718)
                     /locus_tag="ACZPD3_03010"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            4737..5636
                     /gene="cysD"
                     /locus_tag="ACZPD3_03015"
     CDS             4737..5636
                     /gene="cysD"
                     /locus_tag="ACZPD3_03015"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HIR1415008.1"
                     /translation="MSNLSHLDALEAEAIYIMREVAAECEKPVMLYSIGKDSSVMLHL
                     AMKAFYPEKPPFPFLHIDTTWKFHEMIEFRDRIAKKNGIDMLVYTNEEGVKAGINPFD
                     NGSAYTDIMKTQALKQALTKYGFTAAFGGGRRDEEKSRAKERIFSFRNSAQAWDPKNQ
                     RPEMWKLYNTKIQKGESMRVFPISNWTEKDIWQYIQRENIEIVPLYFAKERPVIYRDG
                     NIIMVDDDRLKLRPGEKIENKKVRFRTLGCYPLTGGIESEADTLDEIIDETLSAVSSE
                     RTSRVIDHEAAGSMERRKREGYF"
     gene            5638..>7204
                     /locus_tag="ACZPD3_03020"
     CDS             5638..>7204
                     /locus_tag="ACZPD3_03020"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HIR1415009.1"
                     /translation="MKGLLKFITCGSVDDGKSTLIGHILYDAKLLYTDQEKALELDSK
                     VGSRGGAIDYSLLLDGLMAEREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAV
                     GASFADLAVILVDASQGVLVQTRRHARICALMGIRYFVFAVNKMDLIEYDEKRFRDIE
                     NQIAALIEELKLANVTIIPVSATEGDNVTTKSDNMPWYKGEALLSHLETVDIKEDTEK
                     GFYMPVQRVCRPNHEFRGFQGQIENGAIRAGDLVTSLPSKEEAHVKSILVGDKEVQEA
                     VQGQPVTIQLDREVDVSRGCVLTIDSGAVLTDSVEADILWMDDNALTDGKNFFVKIGT
                     KMIPGLVTKINYSVDVNTGEKKSAYTLKKNEIASCTLEFSEKIVVDEFDRHRTLGELI
                     LIDRVTNMTSACGVVRKTFVSQDRSQIGKVDEQVRAGLKGQTPVVVEFPIGKEGITLD
                     FAEQVEKGLTVLGKHTYLYHPAASENYAETVRHLKAAGLIVLLVLDENTAKDETLKTL
                     DGFYANWQIDGITV"
BASE COUNT         2221 a         1308 c         1879 g         1796 t
ORIGIN      
        1 atcgcataca caagaatagg tattacagtg gctatgatat ttgtaggaat accgttcgtt
       61 gtgcgaagtg tacagccggt tctggaaaag atggataatt cttacagcga ggcagcagga
      121 gtgctgggag caaagaaaac aactatcttc tggaaggtca tttttccgga actgaagccc
      181 gcgatctttg cgggaaccgg acttgcgttc ggaagatgtc tgggtgaata tggaagtgtt
      241 gtatttatcg caggaaataa accctattat acagaaatca caccgctggt tattatgtca
      301 gagcttcagg aatttgatta ttcaagtgca acggcaatcg cacttgtgat gttggcagtt
      361 tcatttttca ttctgtttct taacagtatg atacagcaga gaaatgcaag aattttaaga
      421 ggaggaaatg cctgatggac aacaatgaaa agacttcgga gaaaattaca aaatggctgc
      481 ttgtcgggat cagtgttctg tttctggcag tcatgctcct tcttcctctg atcactgtga
      541 tcacagaagc attgcggagc ggatggaagg tctatgcatc tgcagtgaca gatgagtaca
      601 ccatcaaagc acttcttctt acagtgaagg caacactctt tgcagttggt ttcaacacca
      661 tttttggtat ttttgcagcc tgggcactga cgaagtttca gttcaggggt aaaaagtttc
      721 tcactaccct gatcgatctt ccggttacta tttcccctgt tattgcaggt ttgatctttc
      781 ttctgatttt cggacgacag agtcctgttt acttttttct catgaagtta gggattaaag
      841 ttgtattctc tgtaccggga atcattattg caactgtatt tgtaaccttt ccttttattt
      901 ccagagaact gattccggtt ctggaagcag aaggaagcga tgaggaggaa gcggcagccc
      961 tgatgggagc aggtggatgg accattttct ggaagatcac attccctcat atcaaatggg
     1021 cattactgta tggagttgtg ctctgtacgg caagagcaat gggagagttt ggagcggtat
     1081 ctgtactttc cggacattta agaggaaaga ccaatacact tcctcttcat gtagagattt
     1141 tgtttaatga gtttcagtat gttccggcat ttgccgtatc ttcactgctt gtgatcatgg
     1201 cagttatcat tctggtagtc agaagcctga ttgaatataa aggaaagaag gaagcctgat
     1261 atgtatgtag agttaaaaaa tatcaataag acatatggtt cttaccaggc atcaaggaat
     1321 gtgaatctcg gaattgagaa aggtaaactg attggtctgt tgggaccaag cggaagcgga
     1381 aaaacaacta ttcttcgaat gatcgcaggt ctggaaaccc cggacagcgg agaggtaatt
     1441 attgatggaa aagtggtaaa tgatgttccg gccagtcagc gtgggattgg atttgtattt
     1501 cagaattatg cgttgtttcg ctatatgaca gtttatgaca acgttgcatt tggtttgaag
     1561 gttcagaagg ctgataagaa gaaaattgat gccagagtca gagagttgat caaactggtg
     1621 gggctggaag gactggaaaa aagatatcca agccagttat ccggcggtca gaggcaaagg
     1681 gtggcatttg ccagagccct tgctccaaat ccccaggtgc tgcttctgga tgagcccttt
     1741 gcagccattg atgcaaaaat ccgtcaggaa ctgcgaagct ggttaaaaga gatgatcggg
     1801 aaactgggaa ttaccagtat tttcgttact catgatcagg atgaggccat tgaagtagca
     1861 gatgagatta ttatcactaa tgcaggacgg atcgaacaga aaggaactcc cataggggtt
     1921 tacagaaatc ctgagacagc ttttacagcg tccttcttcg gacagccatc tgtgttgaaa
     1981 aatgcggatg atttccatac ctttgctcag gcagaaggag cagacaagat cattgtccgc
     2041 ccggaatttg taaaaatctc caaactggat gaggtggaga agttcaaaac ttccgtatcc
     2101 cggggagtgg ttgaacgggt ttctttcagg ggagataatc tggaacttca ggtgagagtc
     2161 aacaattccg tagtaactgc cagaagaagc ctggagaaag cagatatccg ggaaggtgag
     2221 actgtgaatg tattccttta caggattttt gcactgagag gtgacagtgt acagctgatc
     2281 gaaaatgagg ctgtaaggga tgactttgta attatctgac aacggtggct gcggcgactg
     2341 acgacaatga agaaataaga aattcagagg caagactgat cggaaaatca acttgttggc
     2401 acactggtat ggaggaaagg gtaagagaga atgagaacag agagaatcag gacagatgtg
     2461 ctgatcatcg gcggcggtac ggcaggatgc tttgcagcct atgtgattgg ccaaaaaagc
     2521 ggcagaaaag ttctgatcgc agagaaagcg aatattaagc gaagcggatg tctggcggcg
     2581 ggcgtgaatg cattgaacgc atatattaca gaaggcagag tgccgcagga ttatgtggat
     2641 tatgccaaaa aggatgccca tggaatcgta agagaagacc ttcttcttac catgtcagag
     2701 agactgaatc atgtgacaaa ggtgatggaa gatctggggc tggtcatcct gaaggacgaa
     2761 aacggaaaat atgttgccag aggaaaccga aacatcaaga tcaatggcga aaatatgaag
     2821 ccaatccttg caaaggcggc agctgaacag gagaatgtta cggttctcaa tcatgtaaat
     2881 attacagatt ataaaacaga aaacaaaaaa gtcacaggcg cagttggatt tagcgttaat
     2941 gaggatattt tctatgacat tgatgcaaaa gcagttcttt gtgccacagg tggtgcagcg
     3001 ggactttaca gaccgaataa tccgggattt tccagacata agatgtggta tcctccgttt
     3061 aacacaggtg caggatatgc aatgggactt ctttccggtg cagagatgac aacctttgag
     3121 atgcgtttca ttgcattgag atgtaaggac acgattgctc ctacaggaac tattgctcag
     3181 ggcgttgggg ccagacaggt caatgcacat ggtgatattt atgaaacaaa atatggtctg
     3241 acaacttccc agcgagttta cggtacagtc atggagaaca gagaaggaaa cggtccatgt
     3301 tatctcagaa cggaaggtat ttccaaagaa caggagcagg acctttacaa agcttacctg
     3361 aatatggcac cgtcccagac tctgaaatgg atggaagccg gaaaaggacc atccgaagag
     3421 aatgtagaga ttgagggcac agagccatat attgtaggcg gacatacagc cagcggttac
     3481 tgggtagata ataacagagc tacaactatt gaaggtttat atgcagccgg agacgtagca
     3541 ggaggatgtc cgcagaaata tgtaaccggc gcactggcag aaggtgagat cgcagcagag
     3601 gcgattttgg aatatctgaa tggcaaagat gataaccgga tcgcagcgga aaataaggaa
     3661 ggggctttag agagtggcgg agaacaggag ctgtctcaaa ctacaatagc taagaaggca
     3721 gaatacgaat cacatttgaa tgcttcccgt tctttgatga atgcagagca gcttgaggaa
     3781 gccatgcaga agatcatgga tcagtacgca ggaggaatcg gtacagacta cagatacagc
     3841 gggatgagtc ttgcaaaggc agatgaaaag attgcagcat tgttgccagt agttgacact
     3901 ctggcagcaa cagatactta tgaactgatg cagatttatg aacttcgcga acgactgatc
     3961 gtgtgtcagg cagtgatcgc acatctggca gccagaaagg aaaccagatg gcatagtttc
     4021 ggagagaata cagactaccc gcagcagagc gaagagtgga tgaaatatgt aaactcccgt
     4081 atggaaaacg gcgagattaa gatcttatat cgtgacctga tcacaaagga aaatacaggt
     4141 ttgaatacag cagagaaagg agcaggtgag agatgagcat tcggattcaa aaaagtaaat
     4201 gtgcaggatg tggcagatgt atcgaagcct gtcccggaaa tctgatcaaa aaggataaag
     4261 agaacaaagc ctttatccgt caggtaagag actgctgggg atgtacatcc tgtatcaagg
     4321 aatgcaggca tgatgccatc cgctttttcc ttggagctga tgtggggggc agaggtgcaa
     4381 gtatggttgt atcagagaaa ccggatattt ccacatggac agtggagaag ccggatggca
     4441 ctaagatcac tattcaggtt aataagaaag acgctaataa atattaaaca taagtatgta
     4501 ttctcagagt ctttagagtg tgtttgaaaa atgctttacg caagatgtgc gtcacaattt
     4561 gtgggagatt ttgttcggat gagggcgtcg tagcgggcta cgacaaccga attcggacaa
     4621 aatataccgc aaagtggggc gtgcagattg cgggaatgat ttttcagaca cgctctagtg
     4681 tatttgctca agtacgaaaa gattccgagt ataaacaaac agaggaggat aatgcaatga
     4741 gtaacttatc tcatcttgat gcgcttgagg cagaggcgat ttatattatg cgagaagtag
     4801 cagccgagtg tgaaaagccg gttatgctct attctatagg aaaggacagc tccgttatgc
     4861 tccatctggc gatgaaggca ttttatccgg agaaacctcc attcccgttc cttcacattg
     4921 atactacatg gaaattccat gagatgatcg aattccgcga cagaatcgca aagaaaaacg
     4981 gaattgatat gctggtttac accaatgaag agggtgtaaa ggcaggaatc aacccatttg
     5041 acaatggttc tgcatataca gatatcatga aaacacaggc cttaaaacag gctctgacca
     5101 aatatggatt tacagcggca tttggaggcg gccgtcgtga tgaggagaaa tccagagcaa
     5161 aagaaagaat cttctctttc cgtaattcag ctcaggcatg ggatccgaaa aaccagagac
     5221 cggagatgtg gaagctttat aacactaaga tccagaaggg agagagtatg cgagtattcc
     5281 caatttccaa ctggacagaa aaggatatct ggcagtacat tcagcgtgaa aatatcgaaa
     5341 ttgtaccgtt atattttgcg aaagaaagac cggtcatcta cagagacgga aatatcatta
     5401 tggtggatga tgacagatta aaactccgtc cgggtgaaaa gattgaaaac aaaaaagtca
     5461 gattccgtac tcttgggtgc tatccgctta ctggaggtat tgaatctgaa gcagatactc
     5521 tggatgagat tattgatgag acgttaagtg ctgtttcttc tgaacgtacc agcagggtga
     5581 tcgaccacga ggcagcaggc agcatggaga gaagaaagag agaggggtat ttctagaatg
     5641 aaaggattat tgaaatttat tacatgcgga agtgtggatg atggaaaatc cactctgatc
     5701 ggacatattc tttatgatgc aaagcttctc tatacagatc aggagaaagc tctggagctg
     5761 gattccaaag taggaagcag aggcggtgcc attgattatt ctcttcttct ggacggtctg
     5821 atggcagagc gtgaacaggg aatcaccatt gatgttgcat accgttattt tacaacagat
     5881 aacagaagct tcattgttgc agatacaccc ggacatgaag aatatacaag aaacatggca
     5941 gtgggtgcgt cctttgcaga tctggcagtt atcctggtgg atgcttcaca gggagtactg
     6001 gtacagacaa gacgtcatgc aagaatctgc gcattgatgg gaatccgtta ctttgtattt
     6061 gcagtaaaca aaatggatct tattgaatat gatgaaaaac gtttccgcga cattgagaat
     6121 cagatcgcag cactgataga agaactgaaa cttgccaatg taactattat tccggtatct
     6181 gccacagaag gtgacaacgt tacaaccaaa tctgacaaca tgccatggta taaaggtgag
     6241 gcgcttcttt cccatctgga aaccgtagac attaaggaag atacagagaa gggcttctat
     6301 atgcctgtac agagagtctg cagaccaaac catgagtttc gcggattcca gggacagatt
     6361 gagaatggtg ccatcagggc aggcgacctt gtgacttctc ttcccagtaa agaagaagct
     6421 catgtaaaga gtattcttgt tggcgataaa gaagttcagg aagcggtaca gggacagcct
     6481 gttaccatcc agcttgacag agaagtagat gtttcccgcg gatgtgtact taccattgat
     6541 tccggcgcag ttcttacaga ctctgtggaa gcagatatcc tctggatgga tgataatgct
     6601 cttacggatg gcaagaactt ctttgtaaaa atcggtacaa agatgatccc cggcctggtt
     6661 acaaagatca attattctgt ggatgttaat acaggtgaaa agaaaagcgc ttatacctta
     6721 aagaaaaatg agattgcttc ctgtactctg gaattctcag agaaaatcgt tgtggacgaa
     6781 tttgacaggc acagaacact gggtgagctg atcctgatag atcgtgtcac aaatatgact
     6841 tctgcctgcg gtgttgtgag aaaaaccttt gtttctcagg atagatccca gatcggaaaa
     6901 gtggacgagc aggttcgtgc aggactgaaa ggtcagacac cggtagtagt ggaattcccg
     6961 atcggtaaag aaggaatcac tcttgatttt gcagagcagg tagaaaaggg actgactgta
     7021 cttggaaaac atacctatct gtatcatccg gcagcgagtg aaaactatgc agaaactgtc
     7081 cgccatctta aggcagcagg tctgatcgta cttcttgttc ttgatgaaaa tacagcaaaa
     7141 gacgagacat taaagactct ggatggtttc tatgcgaact ggcagattga cggaatcact
     7201 gtga
//
LOCUS       DBMDXG010000061         7158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2706_length_7158_cov_1.639223, whole genome shotgun sequence.
ACCESSION   DBMDXG010000061 DBMDXG010000000
VERSION     DBMDXG010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_7158_cov_1.639223"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            727..2067
                     /locus_tag="ACZPD3_03025"
     CDS             727..2067
                     /locus_tag="ACZPD3_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415010.1"
                     /translation="MKTKIQDMTSGSPGRLIILFAIPLMLGNICQQLYTMVDTMVVGQ
                     IAGVEALAALGAVDFLMWVVTGISTGLTQGFSIQLSQYYGAKDFENLRKSLAHSYRLT
                     AFIAAGVLILSQSFASLVLTGLHTPSNIIGMSLLYLRIIFCGIPATAAYNMFASALRA
                     MGNSKTPLTAMIIASVLNVSLDILFVAGFGWGVAGAAIATVIAQSFSAVYCFLILRRI
                     DIVHLTRADFMPASGMNARLMKLGIPVVFQNIIIGVGGLVVQYVINGYGFLFVAGFTA
                     TNKLYGLLEMAAISYGYAIVTYVGQNLGAGKIDRIRKGVRSSMLLSLLTSLIISAAMF
                     LFGKNILSLFISGEPQQTQQVLAIAFKYLSIMAAILWVLYFLYVYRSALQGLGDTLMP
                     MVSGMAEFVMRISAALILPHFIGQDGIFFAEIAAWSGATVILCISYYVRMHKYH"
     gene            complement(2126..>3025)
                     /locus_tag="ACZPD3_03030"
     CDS             complement(2126..>3025)
                     /locus_tag="ACZPD3_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415011.1"
                     /translation="QTFLAIMKNCGAVNMSTLINGVMVILNIALNAVFIFGLSGFPKM
                     GIKGAALATVLATVVQFLWSVGYVLCRIRAVKFSLRSCEKKLFGRFWQKAVPLLINNL
                     AWGIGFSMYAVIMGHLETDAVAANGIANISKNLVVCFCLGLGNAGSIIVGNRLGADRL
                     QEAKEVGGTLTRTAIIAGIVSGLVLMALSPFITKMVDLTPTARGYLQKMLLISSYYIA
                     GKSVNCMTIGGIFAAGGDSKFGMLCDSVTLWCIIVPLGCICAFILKLPVMVVYFVLNL
                     DEIIKLPVVYKHYKKYKWIKNLT"
     assembly_gap    3026..3035
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3036..3588)
                     /locus_tag="ACZPD3_03035"
     CDS             complement(<3036..3588)
                     /locus_tag="ACZPD3_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415012.1"
                     /translation="MDERKRSLRKEIVRLALPIALQQFMTALVGACDAIMLGKLSQDA
                     MSAVSLATQVTFVFNLFMFAFMAGENMFVAQYYGKGDYTGISQVFSLVTKICGCIAVV
                     FLAGTLFFPEQLMRILTNEETLIVLGSEYLRVIGISYVFSGIAQTFLAIMKSERSFSA
                     FLASGYEARMPDCLTARPSDQEAS"
     gene            complement(3628..6048)
                     /locus_tag="ACZPD3_03040"
     CDS             complement(3628..6048)
                     /locus_tag="ACZPD3_03040"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR1415013.1"
                     /translation="MAKWQRSFFQPVLPLGEDGRRVTGSKEHIALSRMAAGEGMVLLK
                     NEKNTLPIRRGTKVALFGKGTVDYVKGGGGSGDVTVEYIRNLYEGMKIKEDEGKVEVF
                     DKLAKYYAEDIQKQYEDGAVPGMTVEPELPDELLNEAREYTDTAIITICRFSGEGWDR
                     KCEAAQDGYVLDGEEKRNSELSAKIFENGDFCLTNAENAMVEKVKKAFPHVIVVMNVG
                     GIVDTKWFRDCDEIQSVLMAWQGGMEGGLATADILCGDVNPSGKLSDTYAKDLEDYPS
                     TANFHESAFYVDYTEDIYVGYRYFETIPGAAKRVNYPFGFGLSYTDFDWKVTGASEEN
                     GVITVLTEVTNTGKKAGKEVIQLYYGAPQGKLGKPAKVLGAFKKTSILQPGERQILTL
                     KIPVNQMASYDDLGKVCKSAYVLEAGEYAIYIGTNVRDAAKIDFTYVVKEDTVTEQLS
                     RKAAPYHLQKRMLADGSYEELPQREYVEEEGLPRQDKYAIGLPCPDTRGQKGIDFLDF
                     LDSKGVHFSDVVDGKMTLDEFMDILTLDDCINLLGGQPNTGCANTFGMGNLPEYGVPN
                     VMTADGPAGLRILPKCGVNTTAWPCATLLASTWDEGLVEKVGKAGAEEVKENNISIWL
                     TPACNIHRSPLCGRNFEYYSEDPYLAGKTGAAMVRGIQSQHIGASVKHFAANNKETNR
                     KDSDSRVSERALREIYLKQFEIIVKEAHPYTIMSSYNLLNGIHASENKELLTGILRDE
                     WGFDGVVTTDWWTFGEHYRETKAGNDIKMAAGYPERIKEAYEKGFVTEAEIRLSARRI
                     LNMILKID"
     gene            complement(6121..6735)
                     /locus_tag="ACZPD3_03045"
     CDS             complement(6121..6735)
                     /locus_tag="ACZPD3_03045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR1415014.1"
                     /translation="MGQIHNIEKLTDCKFLNLYHLNATSIHNTPVSYFVASRAKSINE
                     LKIKTGKNTPDGVIIYSLYGEKRDRVVLVRQYRYAIGGYIYEFPAGLVEPNEEFHEGA
                     VREMYEETGLKFTPLKVDPAFEKPYFTTVGMTDESCATVYGYAEGEISKDAQEDTEEI
                     EVVLADREEVKRILREENVAIMCAYQLMHFLRDEEPFAFLKEKL"
     gene            complement(6776..>7158)
                     /locus_tag="ACZPD3_03050"
     CDS             complement(6776..>7158)
                     /locus_tag="ACZPD3_03050"
                     /inference="COORDINATES: protein motif:HMM:NF021030.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HIR1415015.1"
                     /translation="DTKHLPGVVTWMNQLQKVELLDKHQMICFSIASVQYGDKDFFVT
                     DEFSDSLTFYAELLTEAGETWKQLALDEIQRCDQIANIVGRLARDLVKSAGGDEGDAA
                     SYRAKEEFYQMFCELKTEIEENWL"
BASE COUNT         1831 a         1716 c         1347 g         2254 t
ORIGIN      
        1 aactgttggc atactcagat tcagttcttt ggatatttcc acttttgatg tggattctct
       61 atttataata taatttacga tttttgattt cgtagcaaat ttcttttcca gtccgctcat
      121 tctgtttcta ccttctttta ttaatgtatt tataaaagaa tgatacctgt tattgtcata
      181 tttgtaaata gccattgtct ctgcagagta tgttgctttt tgcaattgag gcaggggact
      241 taacttattc agctgattca gttaatatat ctacaataac agagttactg ttcactccgt
      301 tctcagtaac acgcttcgcg atgcacagaa tttatgctaa tcgcataaat tcgcgctgta
      361 tgtctcggga ttttggcatg ttcatgccaa aacacctcgc gggatagcgg cgtgtgaaca
      421 gtaacataac agataaattt atatcattct gatgaaatat tatatttcat ttttcacgtt
      481 aatgatataa aattatcgta attgttcagt accttcacag tcacgagcca aaatacatta
      541 aaaatcacct gcggtgatgg cattttggct tgtatgtctc gggattttgg tacttacgtg
      601 ccaaaacacc tcgcgggata gcagactact gaatagttac aaattattaa tgtaaatatc
      661 agggcatatt cgaataatca tttctaaact ttttcaaata tgctccggaa aggaactttt
      721 atactaatga aaaccaagat tcaggatatg acctccggtt caccgggacg gttgatcatt
      781 ctttttgcaa ttcccctgat gctgggaaat atctgtcagc agctttatac aatggtggat
      841 accatggttg ttggccagat tgcaggtgtg gaggctcttg ccgcactggg tgctgttgac
      901 tttctgatgt gggttgtcac aggaatatcc acaggactca ctcagggatt ttctattcaa
      961 ctctcccagt attatggggc aaaggatttc gaaaatctgc gtaaatctct ggctcacagt
     1021 taccgactga ccgcattcat cgcagccgga gtattgattc tcagccagtc atttgcttcg
     1081 ctggtattaa cagggctaca tacaccttcc aatattatcg gaatgtctct gttatatctg
     1141 cgcatcattt tctgtggaat ccccgccaca gcagcctata atatgtttgc ctctgcccta
     1201 agggctatgg gaaacagtaa aacaccgcta accgcaatga tcatcgcatc tgtgctgaat
     1261 gtctcactgg acatcctttt tgtggcaggc ttcggatggg gtgtggcagg agcagcaatc
     1321 gcaactgtga tcgcacagag cttctccgct gtttactgtt tcctgatttt acgcagaatt
     1381 gatatcgttc atctgaccag agcagatttt atgcctgcct ccggcatgaa tgcccgcctg
     1441 atgaagcttg gtattcctgt tgtattccag aatatcatca tcggtgtcgg cggtctggta
     1501 gtccagtatg tgatcaatgg ctacggtttc ctttttgtgg caggatttac agctacaaac
     1561 aagctttatg gtctgctgga aatggcagct atttcctatg gatatgccat cgttacctat
     1621 gtgggtcaga atctgggagc cggaaagatt gacagaatca ggaaaggtgt ccgcagcagt
     1681 atgctgcttt ccctgcttac ctcactaatc atttctgctg ccatgttttt atttggaaaa
     1741 aatattctct cacttttcat ctcaggtgaa ccgcagcaga cacagcaggt acttgcaatt
     1801 gcttttaaat acctctctat tatggctgcc atactctggg ttttgtactt cctgtacgtg
     1861 tacagatctg cacttcaggg gctgggagat accttaatgc ctatggtaag tggtatggct
     1921 gaatttgtca tgcgtatcag tgctgcactg atccttcccc atttcatcgg acaggacggt
     1981 attttctttg cagagattgc agcatggagc ggagccactg tgatcttatg catcagctat
     2041 tatgtacgga tgcataaata tcattaaaac aaaacaggca catagtcccg gatatccggt
     2101 atatgtgcct atgaagcgtg taaaattatg taagattctt aatccattta tatttcttat
     2161 aatgtttata gaccaccggc agtttaatga tctcgtccag attgagtaca aagtagacaa
     2221 ccataaccgg cagtttcaga ataaatgcgc agatgcatcc cagaggaaca atgatacacc
     2281 acagagttac agaatcacac agcattccaa atttggagtc tccgcctgct gcgaagattc
     2341 cgcctattgt catacagttt actgattttc ctgcgatgta ataggaagag atcagcagca
     2401 ttttttgcag atatccgcgg gctgtcgggg tgagatctac catttttgtg ataaatgggg
     2461 ataaggccat caggacaagg cctgatacaa ttcctgcaat aatcgcagtt ctggtaagtg
     2521 tcccgcctac ctcttttgct tcctgcagac ggtctgcacc aagacggttt cccactatga
     2581 tactgcctgc atttccaagt ccgaggcaga agcatacaac aagatttttg gaaatattcg
     2641 ctattccatt ggcggcaaca gcatccgttt ccagatgtcc catgatcaca gcatacatgg
     2701 agaaacctat tccccatgca agattattga tcaataacgg aacggctttc tgccagaaac
     2761 ggccaaataa ctttttctca cagcttctga gactgaattt tacagctctg atcctgcaca
     2821 atacataacc aacactccac aggaactgca caactgttgc aagtacagtt gccagtgcag
     2881 cacctttaat ccccatcttc gggaagccgg acaggccaaa aatgaaaaca gcattcaatg
     2941 cgatattgag aattaccata accccattga tcagggtact catatttact gcaccacagt
     3001 tcttcatgat tgccaggaaa gtctgnnnnn nnnnntgctc gcctcttggt ctgatggcct
     3061 tgccgtcaag caatccggca ttctcgcttc gtacccggac gccaaaaatg ctgaaaaact
     3121 gcgctccgac ttcatgattg ccaggaaagt ctgtgcaatt cccgaaaaca cataggaaat
     3181 gccgatgact cttaaatact cacttcccag aacgatcaga gtttcctcat ttgtcaggat
     3241 tctcatgagc tgttccggaa agaaaagagt tcctgccaga aatactactg caatacatcc
     3301 acaaatcttt gtcacaaggc tgaacacctg ggaaatacct gtgtaatccc cttttccata
     3361 atattgtgcc acaaacatat tctcgcctgc cataaatgca aacataaaca gattaaatac
     3421 aaaagtaacc tgtgtagcca gtgatacggc tgacatagca tcctgactta actttcccag
     3481 catgatcgca tcacatgctc cgaccagggc tgtcatgaac tgctgaagtg cgatcggaag
     3541 tgccagcctg actatttctt ttcgtaaact tcgtttacgc tcatccatta tcttttttgc
     3601 tcctgtataa aatattaatg atactcatca gtcaattttc agaatcatat tcaggattct
     3661 tcttgcgctg agacggatct ctgcttcagt aacaaatcct ttttcatatg cttcttttat
     3721 gcgttccgga tatcctgctg ccatcttgat atcgtttcct gcctttgttt cgcgatagtg
     3781 ctctccgaag gtccaccagt ctgttgtaac cactccgtcg aatccccatt catcccggag
     3841 aatacctgtc agaagttctt tattctctga ggcatggatg ccattaagca gattatagga
     3901 ggacataatc gtatacggat gagcctcttt tacaataatc tcaaactgtt tcagataaat
     3961 ctcacgtagt gctctctcgg atactctgga atcagaatcc ttacggtttg tctccttatt
     4021 attggctgca aaatgcttca cacttgctcc gatatgctga gactgaatgc ctcttaccat
     4081 agctgcacct gtctttcctg caaggtacgg atcctcagaa taatactcaa agttacggcc
     4141 acagagcggg cttctgtgaa tgttgcaggc tggtgtaagc cagatactga tattattttc
     4201 ttttacttct tccgctcctg ctttaccaac tttctctacc agtccttcat cccatgtgct
     4261 ggcaagaagt gtggcacatg gccaggcagt tgtgttcact ccgcatttcg gaagaatgcg
     4321 aagtccggct ggtccgtctg cagtcatcac attcgggact ccgtattccg gaagatttcc
     4381 cattccaaat gtattggcac atcctgtgtt tggctgtccg ccaaggagat tgatacagtc
     4441 atccaatgtc aggatatcca taaactcatc caatgtcatt ttaccatcta ctacatcaga
     4501 gaagtgaact cccttggaat caagaaaatc aagaaagtct attccttttt gtcctcttgt
     4561 atcagggcat ggaagaccga tggcatattt atcctgtctc ggaagtcctt cttcttccac
     4621 atattcacgc tgtggaagtt cctcataaga accgtccgca agcatacgtt tctgaagatg
     4681 atatggtgct gccttccggc ttaactgctc agttactgta tcttctttta ctacatatgt
     4741 aaagtcaatc ttcgctgcat ctcttacgtt tgtaccaata tagattgcat attctcctgc
     4801 ttccagaaca taggctgatt tgcatacttt tcccagatca tcgtaggatg ccatctgatt
     4861 cacaggaatt ttcagggtaa ggatctggcg ttcacccggc tgaagaatgc tggtcttttt
     4921 gaatgctccc agcactttcg caggctttcc gagtttaccc tgtggtgctc cgtaatagag
     4981 ctggataacc tcttttcccg ctttttttcc ggtattggtt acttcagtaa gaactgtgat
     5041 cacaccgttt tcttcggaag caccagtcac cttccagtca aaatctgtgt aggaaagtcc
     5101 aaatccaaac ggataattta ctctctttgc tgcacccgga attgtctcaa agtaacggta
     5161 tcctacataa atatcttctg tataatctac atagaatgca gactcatgga agtttgctgt
     5221 ggatggataa tcttccagat cttttgcata agtatcggat aatttaccgg atggattaac
     5281 atcaccacaa agtatatctg ctgttgcaag accaccttcc ataccgccct gccatgccat
     5341 cagtactgac tggatctcat cacagtctct gaaccatttc gtgtccacga taccgccaac
     5401 attcatcact acgattacat gtgggaatgc tttctttact ttctctacca tggcattctc
     5461 tgcatttgtc aggcagaaat caccgttttc aaaaatcttc gctgacaact cggagtttct
     5521 tttttcctct ccgtcaagaa cgtatccatc ctgtgctgct tcacattttc tgtcccatcc
     5581 ttcgcctgag aaacgacaga tggtaatgat tgcagtatct gtatattctc gcgcttcatt
     5641 cagaagttca tccggaagct ccggctcaac tgtcataccc ggtacggcac catcttcgta
     5701 ctgtttctga atatcttccg cataatattt cgcaagttta tcgaaaactt ctactttccc
     5761 ctcatcctct ttaatcttca tgccctcata aagattacgg atgtattcta cagttacatc
     5821 gccgcttcct ccgccgcctt ttacgtagtc tacagtaccc ttaccaaaga gtgcgacttt
     5881 cgtccctctc cggatcggaa gtgtattttt ctcatttttc agaaggacca ttccctctcc
     5941 tgctgccatt ctggaaagtg caatgtgttc tttacttcct gtaactctcc gtccatcttc
     6001 tcccagcgga agcacaggct ggaagaagct tctctgccat tttgccatta ttttaatcct
     6061 cccttaattc tccttaatca agcagataaa atcatccgct tcgctacttg cctgatttgt
     6121 ttaaagtttt tccttcaaaa atgcaaaagg ttcttcatcc cgaaggaaat gcataagctg
     6181 atatgcacac atgatcgcta cattttcttc acgcagaatc ctctttactt cttctctgtc
     6241 agcaagtaca acttctattt cttcggtgtc ttcctgtgca tctttgctga tctcaccttc
     6301 cgcatatccg taaacagttg cgcaggattc atctgtcatg ccaaccgttg tgaaataagg
     6361 tttctcaaat gcaggatcta ctttcagagg tgtgaatttc agtcctgttt cctcgtacat
     6421 ttctcttact gcaccctcat ggaattcttc gttcggttct accagacctg ccgggaattc
     6481 gtagatatat ccgccaattg cgtatctgta ctgacgtact aatacaactc tgtctctctt
     6541 ttccccataa agactgtaaa tgatcacgcc atccggagtg tttttacctg tttttatctt
     6601 taattcgtta atactcttcg cacgggatgc cacgaaatat gacacaggag tattgtgaat
     6661 gcttgttgca tttaaatggt acaaatttaa aaatttacaa tctgtcagtt tttcaatatt
     6721 gtgtatctgt cccataatgg atacttcctt tcgctttcta taccttcgct gattattaca
     6781 gccaattttc ttctatctct gtttttagtt cgcaaaacat ctgataaaac tcttcttttg
     6841 cacggtagct tgcggcatct ccttcatcgc cacctgcgga ttttaccaaa tctctggcca
     6901 atctgccgac aatattcgct atttggtcgc atcgttgaat ttcatctaat gcaagctgtt
     6961 tccaagtttc tcctgcctct gttaacagct ccgcataaaa tgtaagagaa tcgctgaatt
     7021 catcagttac aaaaaagtct ttatccccgt attggacaga agcaatgctg aaacatatca
     7081 tttgatgttt atccaacaat tctaccttct gtaactgatt catccaagtc acaactcccg
     7141 gcagatgctt tgtatcat
//
LOCUS       DBMDXG010000062         7155 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2707_length_7155_cov_1.456762, whole genome shotgun sequence.
ACCESSION   DBMDXG010000062 DBMDXG010000000
VERSION     DBMDXG010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_7155_cov_1.456762"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="ACZPD3_03055"
     CDS             complement(<1..1347)
                     /locus_tag="ACZPD3_03055"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415016.1"
                     /translation="MSEQRRRGPMGGHGRMMSGEKAKDFKGSMAKLFRYMGRYKFRFI
                     LMFIFAVAGTVFSIVGPKILGKATTELFNGLVAKVNGTGEIDFGKIGMILLWTLGLYV
                     LSACFSFVQGFVMSGISNDVTYNLRKDISKKINRLPLNYYESRTNGEILSRITNDVDT
                     LQMSLNQSLTQLITSVTTLIGVFIMMLSINVWMTLAALLILPVSMFIIQTVMKHSQKY
                     FQDQQSYLGKVNGQIEENFGGHNVVRVFNKENDVVEEFEKDNQKLYESAWKSQFFSGM
                     MMPIMQFVGNLGYVMVALLGGVFVIKKSIEVGDIQSFFQYIRNFTQPIQQIAQVTNLL
                     QSSAAASERVFEFLEEPEESQNEKNPVDINTLTGDVQFEHVKFGYNPDKIIINDFSAD
                     VKDGQKIAIVGPTGAGKTTMVKLLMRFYDLNGGSIKVDGYDIKDFNRSSLREMFGMV"
     gene            complement(1344..3506)
                     /locus_tag="ACZPD3_03060"
     CDS             complement(1344..3506)
                     /locus_tag="ACZPD3_03060"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415017.1"
                     /translation="MIKLMKYLKKSAGYIVLIIALLFLQAYCDLSLPDYTSKIINVGI
                     QQKGIEDSVPDKLRDSTLQNLQIFMDEDQKKEVSQNYTQEDDTWLLNDDISKETRENL
                     NEDFSKAMMMVAAFSEDSEQGQAMAAQMGLPEGTDPLTALAQMPEEAVQQIMSQMDEK
                     LKDMPESIVTQAGVSFVASEYEALGKDVDAIQMHYILMSGIRMLAMALVIMLAAISVT
                     FISARVAGRLGHDLRNSIYRKVMSFSSREYHKFSTASLITRSTNDVQQVQQVMAMMFR
                     IVLYAPILGIGGVLKVLNTDSSMTWILGVAVGLILIVIFVLFQVAMPKFTILQTLIDR
                     LNLVSREILTGIPVIRAFSREKHEEDRFEKANLDLTKTNLFVNRCMTFMMPIMMLIMN
                     GVSVLIIYSGAHAVDNGTMQVGNVMAFIQYAMQIIMSFLMITAMSIMLPRANVAALRI
                     DEVLKTEVSIQDPETPVHPSESVKGEIEFEHVSFAYPEAGENVLTDISFKAKKGQTIA
                     VIGSTGSGKSTLINLIPRYYDVTKGSVKVDGVDVRDMTQKELRDKLGYVPQKGVLFSG
                     TIDSNIRYGKPEITDAQVKEAAEVAQATEFIDTKPEKYESPVSQGGTNVSGGQKQRLS
                     IARAIAKDPEIFIFDDSFSAIDFKTDSQLRKALKEYTKDATTVIVAQRISTILGADQI
                     IVLDDGHMAGIGTHKELMANCEVYQQIARSQLSEEELA"
     gene            complement(3648..4115)
                     /locus_tag="ACZPD3_03065"
     CDS             complement(3648..4115)
                     /locus_tag="ACZPD3_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1415018.1"
                     /translation="MWCPEEEKKEKREEKSMPALFMEINRQYGMRCMQRIREIGIQQG
                     QMPIIMIVYRHNGCSQKEIAEWMGVTPPTVNVSIQRLEKADIVCRKRDDKDQRIMRVY
                     LTENGRKIVEELQQESKAVEKVMFSNFSEAELCLLRRFFGQILDNISEIPVNR"
     gene            complement(4349..5233)
                     /locus_tag="ACZPD3_03070"
     CDS             complement(4349..5233)
                     /locus_tag="ACZPD3_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1415019.1"
                     /translation="MDTNVLKTFIAVCEYSGFSAAAKELGYTQSTVSSQIKQLEKELD
                     VRLFDRYYHKINLTEKGVLVLQQARNILKAQAKMLDSLNSAESIEGEIRLSMSSSVCS
                     RYFKNDFLRFHHQYPEIKVEITENGTEQMFDKLRKNETDLVFTLDRHIYDSDFIICAE
                     QEEQVHFIVAADNPVAGCSWKLSEISQNEFVLTEQAMSYRKILNETLASQSLEIRPVL
                     EIGNPLQICELVKNSSLLSFLPDFISEKYVKDGQIKRLDVAGCPVTVWTQLLLHKNKW
                     RSPAINVFIEFYKEVMQR"
     gene            5401..6735
                     /locus_tag="ACZPD3_03075"
     CDS             5401..6735
                     /locus_tag="ACZPD3_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1415020.1"
                     /translation="MKREQLGSRLGFIMLSAGCAIGCGNVWKFPWMCGQNGGGSFMLI
                     YLLCLVILGIPALVLEFSIGRAAQTSPLFMYRKLEKPGQKWGIFGWFCLLGNIALMAF
                     YTVVCGWIIYYFVQFLRGKNGSLGFSAMISSPSVNVFFLLVTVVIAFFILSFNLQGGL
                     ERVTKYMMSALLVLMLALAVHSLFLKGSGEGMTFYLKPDFSKIDGSVIVGAMNQAFFT
                     LSTGMGGMAIFGSYIGKEHSLMGEAIHVITLDTLVAFLAGVIIFPACFTFNLEVNAGP
                     SLLFDTMAAVFNNMSGGRIWGSLFFLFMVFAAMSTVLGVCENILAMIRELTGWSRPKG
                     SVVCGTGVFLLALTTALGFSVFHFQPFAEGTTWLDFWDFIVSNNILPLGSLILALFCC
                     NKFGWGWDNFIKESNTGKGLKVQSWMKPLFRFVLPIIIAFIYIYGMSTFNWR"
     gene            complement(6722..6847)
                     /locus_tag="ACZPD3_03080"
     CDS             complement(6722..6847)
                     /locus_tag="ACZPD3_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415021.1"
                     /translation="MDKRKIHGAYFFAKSRQIFVSSKAIWECPALAVNDFDFNAS"
     gene            complement(6938..>7155)
                     /locus_tag="ACZPD3_03085"
     CDS             complement(6938..>7155)
                     /locus_tag="ACZPD3_03085"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1415022.1"
                     /translation="VQHVMDIRNMNLKQAVRFLKQMDKQHKKYYEFYTGEKWGKPENY
                     DLCINSANYGLKDTIELIRRMLDQKVH"
BASE COUNT         1834 a         1692 c         1348 g         2281 t
ORIGIN      
        1 caccattcca aacatctctc tcagactgct tctgttaaaa tctttgatgt cataaccatc
       61 taccttaata cttccgccat tgagatcata aaatctcata agcagtttca ccatagttgt
      121 ctttcctgca cctgtgggac ctacaatggc aatcttctgg ccatccttca catctgctga
      181 gaagtcattg ataatgatct tatccggatt gtaaccgaat tttacgtgtt caaactgtac
      241 atctcctgta agagtattaa tatctacagg attcttttca ttctggctct cttccggctc
      301 ctccaggaat tcaaatacac gctcggaagc tgcagcagag gactgtaaca ggttggtgac
      361 ctgtgcgatc tgctggattg gctgtgtaaa gttacggatg tactggaaga aggactggat
      421 atcaccgact tcaatacttt ttttgattac aaacacgccg cctaagagag ctaccattac
      481 atatccaagg ttacctacaa actgcatgat cggcatcatc attcctgaga aaaactgaga
      541 tttccatgca gattcataaa gcttctgatt atctttctca aattcttcta ctacatcatt
      601 ctctttgttg aaaacccgga ccacattatg accaccgaag ttttcttcga tctgaccgtt
      661 tactttaccc agatagctct gctgatcctg aaagtatttc tgggaatgct tcatgacagt
      721 ctggatgata aacatggaaa cgggcaggat cagaagtgct gccagagtca tccacacatt
      781 gatggaaagc atcataataa atacaccgat cagggttgtc actgatgtga tcagctgtgt
      841 cagactctgg ttcagactca tctgcagagt gtccacatca ttggtgatac gggagaggat
      901 ctctccgttt gttctgctct cataatagtt cagaggcagt ctgttgattt tctttgaaat
      961 atcttttctc agattatagg taacatcatt ggagatcccg gacattacga atccctgtac
     1021 aaaggaaaag catgcactta acacatataa tccaagtgtc cataacagga tcatcccgat
     1081 ctttccgaag tcaatttcac cggttccgtt taccttggca accaggccgt taaacagctc
     1141 tgtggtcgcc ttacctaaaa tcttaggccc tacaatacta aacacagttc ccgctactgc
     1201 aaagataaac atcagtataa aacggaattt atatcgtccc atataacgga aaagcttagc
     1261 catggaaccc ttgaagtctt tcgccttttc gccggacatc atacgtccat gtcctcccat
     1321 aggacctctt cttctctgtt cgctcatgcc agttcctcct ctgataactg agatcttgca
     1381 atctgctgat atacctcaca atttgccatc agttccttat gagttccaat acctgccata
     1441 tgtccgtcgt ccagtacaat gatctggtct gcaccaagga tagtactgat cctctgggca
     1501 acgattacgg tagttgcgtc cttggtgtac tccttcaatg cctttcttaa ctgactgtca
     1561 gtcttaaagt caatggcaga gaagctgtca tcaaagataa agatttccgg atcctttgca
     1621 atggctctgg caatggacag acgctgtttc tgtccgcccg aaacattggt acctccctgt
     1681 gatacagggg attcatattt ctccggtttg gtatcaataa attctgtagc ctgtgcaacc
     1741 tctgctgctt cctttacctg tgcatcagtg atttccggct taccatagcg gatgtttgaa
     1801 tcaatggtac ctgagaaaag tacacccttc tgcggcacat atcccagctt atcacgaagc
     1861 tctttctgag tcatgtcacg gacatccaca ccatccactt taacagaacc ctttgtcaca
     1921 tcatagtatc ttgggatcag attgatcagg gtacttttac cacttcctgt acttccgatc
     1981 actgcaatcg tctgaccttt ctttgcttta aaggaaatgt ctgtcagtac attctcacct
     2041 gcttccgggt aagcaaagga tacatgttca aattcgatct cgcctttcac actttctgac
     2101 ggatgtaccg gagtttccgg atcctgaatg gaaacctctg ttttcagaac ctcatcaata
     2161 cgaagcgctg caacatttgc tctcgggagc ataatagaca ttgcagtgat catcaggaag
     2221 gacataatga tctgcattgc atactggata aatgccataa cattaccaac ctgcatggtt
     2281 ccgttgtcta ctgcatgggc accactgtaa ataataagta cagatactcc gttcatgatc
     2341 aacatcataa tgggcatcat aaaagtcata catctgttga caaacaggtt tgtttttgtc
     2401 agatccaggt tcgctttctc aaaacgatct tcctcatgct tctcacggct gaatgcacgg
     2461 ataacaggaa tacctgtcag aatctcacgg gataccagat tcagtctgtc gatcagagtc
     2521 tgtaagatag tgaattttgg cattgccacc tggaacagca caaagatcac aatcaggatc
     2581 agaccaactg ccacaccaag gatccatgtc atggaagaat ctgtgtttaa taccttgagc
     2641 actccaccaa taccaaggat cggcgcgtac agaacaatgc ggaacatcat tgccataacc
     2701 tgctgtacct gctgcacatc attggtgctt cgtgtgatca gggatgctgt ggagaattta
     2761 tgatattctc tgctggaaaa gctcataacc ttgcggtaaa tgctgtttct caggtcatga
     2821 ccaagtctgc ctgccactct ggccgaaata aatgtgacgc tgattgctgc cagcatgatc
     2881 accagtgcca tggcaagcat tctgatacct gacatcagga tgtaatgcat ctgaatggca
     2941 tccacatctt ttcccagtgc ttcatattct gaagcaacaa aggaaacacc agcctgtgtc
     3001 acaatagact ccggcatatc tttcagcttc tcatccatct gactcataat ctgctgtact
     3061 gcttcttccg gcatctgtgc cagtgctgtc aacgggtctg ttccctccgg aaggcccatc
     3121 tgtgcagcca ttgcctgccc ctgctcactg tcttcggaaa aggcagctac catcatcatt
     3181 gcctttgaga aatcctcatt taaattctct ctggtttctt tggaaatatc atcgtttaat
     3241 aaccaggtat catcttcctg tgtataattc tgtgaaactt cctttttctg atcctcatcc
     3301 ataaaaatct gaagattctg cagggttgaa tcccgaagct tatccggcac actgtcttca
     3361 atacctttct gctggatacc tacattaatg atcttggagg tataatccgg cagggaaaga
     3421 tcacagtacg cctgcagaaa cagaagtgct atgatcagga caatgtatcc cgctgatttc
     3481 ttcagatatt tcatcagttt aatcattgct tgattccctc ttttcctatt tttaatatgt
     3541 atgtctgttt aaactcacgt gtcagttctt tcacagacaa tattattttt tatctgaaca
     3601 tgctgataca gttttctcaa tctgctccat catgtatttt tcagtttcta cctgttgaca
     3661 ggtatctccg aaatattatc cagaatctgc ccaaaaaacc ttcgtaaaag acatagctct
     3721 gcttcgctga aattgctgaa catcactttc tccacagctt tgctttcctg ctggagctcc
     3781 tccacgattt ttcttccgtt ttctgtcaga tatacacgca tgatgcgttg atctttatca
     3841 tcacgtttcc tgcagacaat gtctgctttc tccagacgct ggatacttac atttacagtg
     3901 ggcggtgtta cacccatcca ctctgcaatc tccttctggc tgcagccgtt atgtctgtat
     3961 acgatcataa tgatcggcat ctgtccctgt tggataccga tttcccgtat acgctgcata
     4021 catctcatac catattggcg gttgatctcc ataaagagcg caggcatgct tttctcttct
     4081 cgtttttctt tcttctcctc ttccgggcac cacataactt cactcctttc cggcctctgt
     4141 cctctttcag gtctgatatt attctttaat tagcctgact aatcatatat aatatacttt
     4201 tagacaaaaa atgcaagtgt tttttgtgca gtttataatt attttagatt tagctttgat
     4261 atttccaatt tgtaaatatt caattttatt ttgcctggct aatttttttc atctcaggtc
     4321 gaataaaatt tccaatactc ttcaagctct atctctgcat aacttcttta taaaattcta
     4381 taaacacatt aattgccgga gaccgccatt tatttttgtg gagcagcaat tgtgtccata
     4441 cagttaccgg gcagccggca acatccagtc ttttaatctg tccgtctttc acatatttct
     4501 ccgaaataaa atccggaaga aaggaaagca ggctgctgtt ttttaccagt tcacatatct
     4561 gaagcggatt tccgatttca agaaccgggc ggatttccag tgattgggat gcaagagttt
     4621 cattcagtat ttttctgtag ctcatggctt gttctgttaa cacaaactca ttctgagaga
     4681 tttcacttaa tttccatgaa cagcctgcaa caggattatc agccgctaca ataaaatgta
     4741 cctgttcttc ctgttccgca caaataataa aatccgaatc ataaatatgc ctgtccagag
     4801 taaaaaccag atctgtttcg ttcttccgca atttatcaaa catctgctct gtaccgtttt
     4861 ctgttatctc cacttttatt tccggatact gatgatgaaa acgcagaaaa tcatttttaa
     4921 aatatctgct gcagacagaa ctggacatag acaaccggat ctcgccttca atgctttcag
     4981 ccgaattcag cgaatcaagc attttcgcct gtgccttcag aatgtttcga gcctgctgca
     5041 aaactaaaac tcccttttcc gtaagattga ttttatgata ataacggtca aacagcctga
     5101 catcaagttc cttttccagt tgtttgatct gtgaagaaac tgtagactgc gtatatccca
     5161 gttcctttgc agcagccgaa aatccggaat attcacatac tgcaataaaa gtttttaaca
     5221 catttgtatc catagttcat tccttcccat aactttccag tatttttcca ttgaaacatt
     5281 gaatcgttat tttttataaa caatattttc aataatatac aacaaaacaa tcaattttac
     5341 aaatattgtt ttttatctta taatatccct tatatgaact tctgaaggga gcgttatctt
     5401 atgaaacgtg aacagcttgg aagccggctt ggcttcatta tgctttccgc cggctgcgcc
     5461 attggctgcg gaaatgtatg gaaattcccg tggatgtgcg ggcaaaatgg cggcggcagc
     5521 tttatgctga tctatcttct gtgtctggtc atcctgggaa tcccggctct tgtgttggaa
     5581 ttttccatcg gaagagcggc tcagacaagt ccccttttca tgtaccgtaa gctggaaaag
     5641 ccaggacaga aatgggggat tttcggttgg ttctgtctgc ttggcaacat tgccctgatg
     5701 gcattttaca ctgttgtctg cggctggatc atttattatt ttgtacagtt tctcagaggt
     5761 aaaaatggtt cactgggatt ttcagccatg atctcctctc cctctgtgaa tgtattcttc
     5821 ttactggtca ctgtagtcat tgcatttttc atcctttcct tcaatcttca gggaggactg
     5881 gagcgtgtga caaaatatat gatgtctgca ctgctggtac tgatgcttgc tctcgctgta
     5941 cacagtcttt ttctgaaagg ctctggtgag ggaatgacct tttatctgaa accggatttc
     6001 tcaaagattg atggttcggt gatcgtaggg gcaatgaacc aggcattctt tactttgtct
     6061 acaggtatgg gcggtatggc tatctttggc agctatattg gcaaagagca ttctctaatg
     6121 ggggaagcta ttcatgtgat cacactggac acattagttg cttttctggc aggagttatt
     6181 attttccctg catgctttac ctttaatctg gaagtaaatg caggtccgag ccttctgttt
     6241 gatacaatgg cggctgtttt taacaatatg tccggaggac gtatctgggg atccttattc
     6301 ttcctgttca tggtatttgc agccatgtca accgtcctgg gtgtgtgcga gaatatcctg
     6361 gcaatgatac gtgagcttac cggctggtct cgtccaaaag gcagcgtggt ctgcggcaca
     6421 ggtgtctttc ttctggcact taccacagcc cttggtttca gcgttttcca cttccagcca
     6481 tttgccgaag gaactacctg gcttgatttc tgggatttta ttgtttccaa caatatttta
     6541 ccccttggct ctctcatact tgcactcttc tgctgtaata agttcggctg gggatgggat
     6601 aactttatca aagagagcaa taccggtaag ggcttaaagg tacagagctg gatgaaaccg
     6661 ctcttccgat ttgtacttcc gattataatt gcctttatct atatttatgg tatgagtaca
     6721 ttcaactggc gttaaaatca aagtcgttta cagcaagcgc cgggcattcc cagattgctt
     6781 tgctgcttac aaatatctgc ctggacttgg caaaaaaata agctccgtgg atttttcttt
     6841 tatccacaga gcttattttc tttgtcaggc ggatattcgc aatccgctcc gctatttact
     6901 tgattcggtt aaatcagaca gataaatctc cctctgatca gtgaaccttc tgatccagca
     6961 ttctccggat cagctcaatt gtatccttca gtccatagtt ggcactgttg atacacaaat
     7021 cataattctc cggttttccc catttctcac cggtataaaa ttcataatat ttcttatgct
     7081 gcttatccat ctgcttcaga aaacgtactg cctgtttcag attcatatta cggatatcca
     7141 taacgtgctg cacac
//
LOCUS       DBMDXG010000063         7080 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2740_length_7080_cov_1.869466, whole genome shotgun sequence.
ACCESSION   DBMDXG010000063 DBMDXG010000000
VERSION     DBMDXG010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2740_length_7080_cov_1.869466"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="ACZPD3_03090"
     CDS             <1..510
                     /locus_tag="ACZPD3_03090"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1415023.1"
                     /translation="SEDSGINEKLFGRADENLKTSLLKRLAGVKNIYNGKLIPPEDGD
                     FVSDDNLFNYQAKIATDLAEKESCVIVGRCVNYVFKDRPNTLRVFVHAPWEFRVEKSR
                     EKISGSDEEIEKFLRRDDKRKQDYYRKFAGGDWSDATNYDLCLNAGKLGFEKCVDAIL
                     AQMNVMGIK"
     gene            complement(552..1871)
                     /locus_tag="ACZPD3_03095"
     CDS             complement(552..1871)
                     /locus_tag="ACZPD3_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR1415024.1"
                     /translation="MDLFEYAKSKTLDQESPLASRLRPTTLDEVVGQQHIIGKNTLLY
                     RAIKADKLTSVIFYGPPGTGKTTLAKVIANTTSAEFTQINATVAGKKDMEAVVKEAQQ
                     NLGMYGKKTILFIDEIHRFNKGQQDYLLPFVEDGTIILIGATTENPYFEVNAALISRS
                     IIFELKSLEISEVKELILRAVNDKTKGMGNYKARIDEDALDFLADMAGGDARNALNAI
                     ELGILTTPRSEDGLIHITLDVASQCIQKRVVRYDKNGDNHYDIISAFIKSMRGSDPDA
                     AVYYLAKMLYAGEDVKFIARRIMILASEDIGNADPQALCVAVAAAQAVERVGMPESQI
                     ILSQAVTYMACAPKSNSAVNAIFAAMDSVKRTKTTVPPHLQDAHYGGHEKLGHGIGYE
                     YAHDYPNHYVHQQYLPTEIQGEHFYELSEMGYEKTLKEYQNKIKSQS"
     gene            complement(2073..2585)
                     /locus_tag="ACZPD3_03100"
     CDS             complement(2073..2585)
                     /locus_tag="ACZPD3_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR1415025.1"
                     /translation="MATPFTVYKLIVLYMLQNTENTLTNSQISEFILDREYTNYFHLQ
                     QAISELVEAELITMDTRSNVSHYRITEDGIKTLSFFQKDLSPEIKQEVREYLKSTGFK
                     AQERIVTPADYYITKQGTYSVRCQLIEKGNSLIDLNIAAPNLEAAQSICKKWSTHYQE
                     IYGKIMEELL"
     gene            complement(2622..3548)
                     /gene="mnmL"
                     /locus_tag="ACZPD3_03105"
                     /gene_synonym="ytqA"
     CDS             complement(2622..3548)
                     /gene="mnmL"
                     /locus_tag="ACZPD3_03105"
                     /gene_synonym="ytqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706535.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA modification radical SAM protein MnmL/YtqA"
                     /protein_id="HIR1415026.1"
                     /translation="MTKWGEKPYHSLDYMLRERFGEKVYKVTLNGGMSCPNRDGKIGT
                     RGCIFCSAGGSGDFAADAALSITDQIESQISILSQKRPIHKYIAYFQAYTNTYAPVEY
                     LEKIFTEAISHPKIVALSIGTRPDCLSPEIVTLLSRLNKQKPVWIELGLQTIHESTAR
                     YIRRGYPLCVFDNAVKHLRKENIEVIVHTILGLPGENTADILETMEYLNHMDIQGIKL
                     QLLHVLRGTDLAADYEKGLFQTYERDEYISLLINCLEHLRPDMVIHRITGDGPKDLLI
                     APLWASRKREVLNMLHHRMKEEQSYQGRLFSH"
     gene            complement(3545..4252)
                     /locus_tag="ACZPD3_03110"
     CDS             complement(3545..4252)
                     /locus_tag="ACZPD3_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR1415027.1"
                     /translation="MDRLFNMDNKFFTVMGRVADLIMLNVVFLICCLPIVTIGASLTA
                     LHYVTLKMARNEESYIIRSFFKSFKQNFKQATVINLIMLAVAAILYMDLRIVGNIDGT
                     MSQVLYIVFFAFGILYMMVFLYIYPVLAKFYNSIKNTFRNAFLMAIRHLPYTVLMAVI
                     TLLPAGVFFIKSFRIQSMAIMLLCMFGFALEAFINGHFLVKIFDNYIPADTDTQDTDL
                     QNGNSAETSSAENDHTV"
     gene            complement(4377..5129)
                     /locus_tag="ACZPD3_03115"
     CDS             complement(4377..5129)
                     /locus_tag="ACZPD3_03115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1415028.1"
                     /translation="MKRTLIFFEILCMIFFLLFSSADKRTQFLTSLAGPGMLSLVKQA
                     SAKATAFEDFSTDTIAGIPVEEPPRIALTFDDGPNARYTPILLDGLKKRNIRASFFLI
                     GENIEGNEDILLQMRKDGHLIGNHTWDHVQLDKIPAEKARLEIEKTNNRIYEASGIYP
                     SYVRPPFGAWIKDMELSVTMLPVFWDVDTLDWKSKNIDSILSIAQTQVHDGSIILMHD
                     GYQTSVDAALKIADLFTEKGYVFVTADQLLLT"
     gene            5373..6095
                     /locus_tag="ACZPD3_03120"
     CDS             5373..6095
                     /locus_tag="ACZPD3_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1415029.1"
                     /translation="MRYNSVLDLHTHTVASGHAYCSLREMAKAASEKGLEVLGITEHA
                     PSMPGTCHKFYFNNLKVVPREMYGIQLLLGSEVNILDAQGTVDLNEKTLSNMDVVIAS
                     LHTPCMKPASRQENTESYLNVMKNPYVNIIGHPDDGRYEIDYEALVQGAKEYGKVLEL
                     NNHSMDPECTRENAVENDTVMLNLCKKYQVPVVMDSDAHFDLLIGEFNLARDLLEKLD
                     FPEELVLNRSADAIREYVNRKF"
     assembly_gap    6306..6315
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6345..6650
                     /locus_tag="ACZPD3_03125"
     CDS             6345..6650
                     /locus_tag="ACZPD3_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR1415030.1"
                     /translation="MSKKIRTEAVDQLFEAILCLKDKEECYTFFEDVCTINELLSLSQ
                     RFEVAKMLMDKRTYLDISEKTGASTATISRVNRSLNYGNDGYEMVFSRMAEKETKGE"
BASE COUNT         1893 a         1222 c         1841 g         2114 t
ORIGIN      
        1 tcagaggaca gcggaattaa tgaaaaactt ttcgggcggg ccgatgaaaa tttgaaaacc
       61 agccttttaa aacgtttggc cggtgttaaa aatatttata acggaaaact gattccgcct
      121 gaggacggag attttgtcag tgatgataat ctttttaatt atcaggcgaa gattgcaacg
      181 gatctggcag agaaggaatc ctgtgtgatc gttgggcgtt gtgtgaatta tgtgtttaag
      241 gacagaccga atactttgag agtgtttgtt catgcaccct gggagtttcg tgtggagaag
      301 tccagagaga agatcagcgg cagtgatgag gagattgaga agtttcttcg tagggatgat
      361 aagagaaagc aggattatta tcgcaaattt gccggtgggg actggagtga tgcgacgaat
      421 tatgatctgt gtctgaatgc aggaaagctt ggatttgaga agtgtgtgga tgcgattttg
      481 gcgcagatga acgttatggg aattaaatga tgaaaagggc agtctctctt tggaggctgc
      541 cctttttggg attaagactg ggatttgatt ttgttttggt attcttttaa ggttttctcg
      601 tagcccattt ctgagagttc gtagaagtgt tctccctgga tttcggtggg gaggtattgt
      661 tggtgtacgt agtggtttgg gtagtcgtgg gcgtattcgt aaccgatgcc gtggcccagt
      721 ttttcgtggc cgccgtagtg ggcgtcctgg aggtgtggcg ggactgtggt ttttgtgcgt
      781 ttgacggagt ccatggcagc gaagatggcg ttgactgcgg agttgctttt gggggcgcag
      841 gccatgtagg tgactgcctg ggagaggatg atctgggact cgggcattcc tacgcgttct
      901 actgcctggg ctgcggcgac tgctacgcag agggcctgtg gatcggcgtt tccgatgtct
      961 tcggaggcga ggatcatgat tcttcgggct atgaatttga cgtcttctcc ggcgtagagc
     1021 atttttgcaa ggtagtagac tgctgcgtca gggtctgagc cacgcatgct tttgatgaag
     1081 gcggagatga tgtcgtagtg gttgtctccg tttttgtcgt agcggacgac gcgtttctgg
     1141 atgcactgag atgccacatc cagggtgatg tggatgagac cgtcttcgct acggggggta
     1201 gtgaggatgc ccagttcgat ggcattgagg gcatttctgg cgtcgccgcc tgccatgtct
     1261 gcaaggaagt cgagggcgtc ttcatctatc cgggctttat agtttcccat tccttttgtt
     1321 ttgtcgttga ctgcgcgaag gatgagttct ttgacttcgg agatctccag ggatttcagt
     1381 tcgaagatga tggaacggga gattaaagca gcgttgactt cgaagtatgg attctctgtt
     1441 gtggcaccga tgaggatgat ggttccgtct tctacgaatg ggaggagata gtcctgctgg
     1501 cctttgttga agcggtggat ctcgtctatg aagaggattg tttttttgcc gtacataccc
     1561 aggttttgct gggcttcttt tacaactgct tccatatctt ttttgcctgc tacagtggca
     1621 ttgatctggg tgaattctgc actggtagtg ttggcgatta cttttgccag ggtggtcttg
     1681 ccggttccgg gagggccgta gaagatgacg gaggtcagtt tgtcagcttt gatagccctg
     1741 tataataagg tgttttttcc gatgatgtgt tgctgtccta caacttcatc cagagttgtg
     1801 gggcgtagtc tggatgccag aggggattcc tggtccaggg tttttgattt ggcgtattca
     1861 aataaatcca tagttttttc tccatagaat ctgatgataa aacagtcaca tttcatttcg
     1921 tgtttatagt aacataaaac atttctgaca aattatagca agtctcttaa tcaatataca
     1981 ttatttacga ggagtgctat agaacgtata tttgttttag tataacgtat ccctgttttt
     2041 ttgtaaagat taggaaataa aaaggaagag cattataaga gctcttccat tattttaccg
     2101 tatatttcct ggtaatgtgt ggaccatttt ttacagatcg actgtgctgc ctcaaggtta
     2161 ggagctgcga tgttcaggtc gatgagggag ttgccctttt cgatgagctg gcatcttaca
     2221 gaataggttc cctgtttggt gatgtaataa tcggcaggag tgacgattcg ctcctgggct
     2281 ttgaagccgg tagatttcag gtattctctg acttcctgtt tgatctcagg agagagatct
     2341 ttctggaaaa aggagagagt ctttatgcca tcttcggtaa tccggtaatg ggagacattg
     2401 gaacgggtgt ccatggtgat cagttctgcc tctaccagtt ctgagattgc ctgctggaga
     2461 tggaaatagt ttgtatattc ccggtccagg ataaattccg aaatctgcga atttgtcagg
     2521 gtattttcgg tattctgaag catgtataac acgattaatt tgtaaactgt gaatggtgtt
     2581 gccattttat ccctcctttg catagagtag cagagaatct tttagtgtga aaacagtctt
     2641 ccctggtagc tttgttcttc tttcatcctg tgatgcagca tatttaatac ctcacgttta
     2701 cggcttgccc acagtggtgc gatcagcaga tctttggggc cgtcgcctgt gatgcggtgt
     2761 ataaccatat ccggtctgag atgttccaga cagtttataa ggagagagat gtattcatcc
     2821 cgttcgtaag tctgaaacag tcctttctcg taatctgctg ccagatcagt gccgcgaagc
     2881 acatgaagta actgcagttt gattccctga atatccatat ggttgaggta ttccatagtt
     2941 tccagaatat ctgctgtgtt ttcaccgggc aatccgagga ttgtatgcac gattacttcg
     3001 atattttctt ttcgcaggtg ttttacggca ttatcaaaaa cgcagagagg atatcccctt
     3061 cggatatacc tggcagtgga ctcatggatc gtctgaagtc caagttcaat ccagacaggt
     3121 ttctgtttat ttaaccggga caaaagtgtc acgatttcag gactgaggca gtctggtctg
     3181 gtgccgatgg aaagagcaac tatctttgga tgagagattg cttctgtaaa aattttctcc
     3241 agatattcca caggtgcgta ggtatttgtg tacgcctgga aataggcaat atatttatga
     3301 atcggacgtt tctgtgaaag gatactgatc tggctttcta tctggtcagt gatggaaagt
     3361 gcagcatctg cagcgaaatc tccgcttcct cctgcgctgc agaagatgca gccgcgggtt
     3421 ccaatcttac cgtctctgtt gggacaggac atgccgccat ttagcgtgac cttataaacc
     3481 ttttctccaa accgctctct cagcatatag tccagagagt ggtatggttt ctctccccat
     3541 ttggtcatac tgtgtgatca ttttctgctg atgaagtttc agcagagttt ccgttctgaa
     3601 ggtctgtatc ctgggtatct gtatcagccg gaatataatt atcaaaaatc tttaccagaa
     3661 aatgtccgtt gataaatgcc tccagtgcaa atccaaacat gcacagaagc atgatggcca
     3721 tggactgtat gcggaaactt ttaataaaaa acacgcctgc aggtaacaga gtgatgactg
     3781 ccatcaggac tgtatacgga agatgacgga tagccatcag gaatgcattg cggaaggtgt
     3841 ttttgatgga gttgtagaat ttggcaagca ccggataaat atacagaaat accatcatat
     3901 aaaggatacc aaatgcaaag aaaacgatat acagtacctg agacattgtt ccgtcgatat
     3961 tgcctacaat acgcagatcc atgtaaagga tagctgcaac tgcaagcatg ataagattga
     4021 tgacagttgc ctgtttgaag ttctgcttaa aggatttgaa gaagcttctg ataatataag
     4081 attcttcgtt acgtgccatt ttcagtgtga catagtggag tgcagtcagt gacgcaccaa
     4141 tagtcacgat tggcagacaa cagatcagaa agaccacatt aagcataata agatcagcca
     4201 cgcgtcccat gacggtgaag aatttgttgt ccatgttaaa tagtctgtcc attcgtttaa
     4261 ttactccttt tgtatataat gatgtacagc atactctgcg gataatcctg ctacagagaa
     4321 atctgtacaa gctttgtata acagtataac gaatcaggca ggaaaaagca aatcttttat
     4381 gtgagcagga gctggtctgc agtgacaaaa acatagcctt tttcagtaaa aagatcagcg
     4441 attttcaggg ctgcatccac agatgtctga tagccgtcat gcatcaggat gatagaaccg
     4501 tcatgaacct gtgtctgggc gatagacagg atagaatcga tgtttttgct tttccagtcc
     4561 agggtatcta catcccagaa tacgggaagc atggtgacag aaagttccat atccttgatc
     4621 catgcgccga aaggcggtcg cacataagaa gggtaaattc ctgaagcttc atagattctg
     4681 ttatttgttt tttcaatttc cagtctggct ttctccgccg ggatcttgtc aagctgtaca
     4741 tgatcccagg tgtgatttcc tataagatgt ccgtcttttc gcatttgcag gagtatatct
     4801 tcgtttcctt caatgttctc gcctatcaga aagaaagatg ccctgatgtt tctttttttt
     4861 aatccatcca gaagtatggg ggtataccgg gcattgggac cgtcatcaaa ggtcaatgcg
     4921 attctgggag gttcttcaac gggaatcccg gcaatggtgt cagttgaaaa atcctcaaaa
     4981 gctgtagctt ttgcagatgc ctgttttaca agggaaagca taccgggacc tgccagtgaa
     5041 gtaaggaact gtgtgcgttt gtcagcagaa gaaaagagca ggaaaaaaat catgcataat
     5101 atttcaaaaa atattaaagt ccgcttcatg caaagatact ccttataaat tatcaaaaat
     5161 atgcagatat ggatgttaat atgttttggg gagattgtgg gatagttata agctgatgta
     5221 atagtatatg aaataaagaa ggaaaagatg tagggagtct gtcaggggag tgatgatgtc
     5281 tcagtatttt gaagtgtaaa atacttgcgg gatgttagga aatggtcgta taattaggga
     5341 taaatatttg tcgaagtgag gagaggtttg ttatgagata taattctgtg ctggatctgc
     5401 atacgcatac tgtggccagt gggcatgctt attgttctct gagggagatg gcgaaggcag
     5461 cttctgagaa gggactggag gttctgggga ttacggaaca tgcgccgagt atgccgggga
     5521 cttgtcataa gttttatttt aacaatctta aggttgttcc aagagagatg tatgggattc
     5581 agctgctgct gggatccgag gtgaatattc tggatgcgca ggggactgtg gatctgaatg
     5641 agaaaacgct ttccaatatg gatgtggtga ttgccagtct gcatactccc tgtatgaagc
     5701 ctgcgtccag acaggagaat acagaaagct atctgaatgt aatgaagaat ccgtatgtaa
     5761 atattatcgg gcatccggat gacgggagat atgagatcga ttatgaggca ctggttcagg
     5821 gtgcgaagga atatgggaag gttctggaac tgaataatca ttctatggat ccggaatgta
     5881 ctcgtgagaa tgctgtggaa aatgatactg tgatgctgaa tctgtgtaag aaatatcagg
     5941 ttccggtggt gatggacagt gatgcccatt ttgatcttct gatcggggag tttaatctgg
     6001 cgagagatct gctggagaaa ctggattttc cggaggagct ggttcttaac cggtctgcgg
     6061 atgcaatcag ggagtatgtg aatcgtaaat tctgataaac tgagaagcag ttatttggtg
     6121 tagagctgcg tcacaaagag gatgatttta tctgccttac tcttcgaaag aggtttttgt
     6181 gagaaaataa aaaaatctgt aatttctttt aggttaaagg cttgcttcct ataatttaac
     6241 gtggtaaaat atcactgtta gaaatataac acggacattt taaagacaaa tgtccgccca
     6301 gcgaannnnn nnnnnaagta cagagttcta aaaggagcat gaaaatgagt aaaaaaatca
     6361 gaacagaggc tgtagatcag ctttttgaag cgattttatg tctgaaggat aaagaagagt
     6421 gttatacttt ctttgaagat gtatgcacga tcaatgaact tctttcctta tcacagcgtt
     6481 ttgaggttgc caagatgctg atggacaaga gaacctatct tgatatttcg gagaaaaccg
     6541 gagcttccac agcaacgatc agtcgtgtga atcgttcatt gaattatgga aatgacggat
     6601 atgagatggt tttttcaaga atggcagaga aggaaactaa aggggaataa gatagcggaa
     6661 ggctgaataa atgcagtgct attgtggggc aggtgaagat cacctgcctt ttttgtatag
     6721 ggagttttcg gataaaagag aagtccgatt attcctcttc tgggagagtg ttgtgcaggc
     6781 tgagaaaatg ctgcgaagga tcaatctata aaagtcgaaa atgaatatta tatagtcaaa
     6841 tatgcaaact atgcggattg gtataatgtt ataattttgt ttatgaaaga taactggctg
     6901 ttggcaatat cgtataagat atttttatga aaaaagtcat atataaagta ttttgctgga
     6961 agtactttac aaatgcatgg cgaaatgtaa tattgcaggc agagagaaca gttataaaaa
     7021 aacaaatcat gaggagaaca taagagtatg aaggcatttg attattcact ggtaaaagat
//
LOCUS       DBMDXG010000064         7039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2755_length_7039_cov_1.715912, whole genome shotgun sequence.
ACCESSION   DBMDXG010000064 DBMDXG010000000
VERSION     DBMDXG010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2755_length_7039_cov_1.715912"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="ACZPD3_03130"
     CDS             complement(<1..498)
                     /locus_tag="ACZPD3_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1415031.1"
                     /translation="MNLYHLRYFSTLAHIEHYTKAADILAITQPSLSYAISTLEEELG
                     VKLFEKNGRNVTLTKYGKVFLNDVEEVLNRLDSSVNSLKLAGKGEGCIDVAFLRTLGI
                     DFLPKIMRGFLAANPTKKIDFNLYCDKVLTDDILNGLKEKKYDLGFCSMIDNEPLIEF
                     IPVARQ"
     gene            796..1638
                     /locus_tag="ACZPD3_03135"
     CDS             796..1638
                     /locus_tag="ACZPD3_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR1415032.1"
                     /translation="MSKEFPITISSWTLGDQCKFEDRVIAAKNAGYEGIGLRAETYVD
                     ALNEGLFDKDILAILDKHGMKVTEVEYIVQWAEEHRSYEQKYKEQLCFHMCELFDVKQ
                     INCGLMENYSVEYTAQKLRELCQRAGKYIIGVEPMPYSGIPDMKKGWAVVKAADCENA
                     KLIMDTWHWVRANQPVDLSVIEDIPADKIVSIQINDVWERPYATTILRDESMHDRLAP
                     GTGIGCTAAFVKMVKEKGIKPNAIGVEVISDAILAKGLEYAANHTYENTKKVLEEAWP
                     EVLQ"
     gene            1774..3699
                     /locus_tag="ACZPD3_03140"
     CDS             1774..3699
                     /locus_tag="ACZPD3_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1415033.1"
                     /translation="MKFNAMFQPINIGPMTVKNRFVVPPMGNNFANTDGSMSEQSAAY
                     YRERAKGGFGLITIEATVVHKGAKGGPRKPCLYDDSTIESFRKVVDACHAEGAKVSVQ
                     LQNAGPEGNAKNAGAPIEAATSIPSDCGRDTPKEVTTEEVYELVKGYGLAAKRAMEAG
                     VDAVEIHMAHGYLVSTFLSPRTNKRLDEFGGSFENRMRFSRLIIEEVKKMTEGKIAVL
                     ARINSCDEVPGGLDVHDSAAIAAYLEGCGLDAIHVSRAVHIRDEFMWAPTVTHGGFSA
                     SQVEEIKRAVSIPVITVGRYTEPQFAELMVKEGRCDLVAFGRQSLADPYMPLKAQEER
                     LEDMIPCIACLQGCVANMYAGNPVCCLVNPFLGHEAEGIAPAEKAKKVMVIGGGVAGL
                     CAAFIAQEKGHQVTLYEASDKLGGNMRLAAYPPGKGDITNMIRSYIVRCQKAGVTIKM
                     NQEVTLDLIREEKPDSVIVASGSRTLILPIEGIDNPAIIHGSDLLDGKRAAGKKVLVV
                     GGGMVGCETAAFLGEQNHDVTVIEFRDTVGADVIHEHRVYLMKDFEDYKIKEITGAKV
                     CKFYEDGVEYETADGQRHESRGYDSVILSMGFRNYNPFGEEIKAIVPDTYVIGDATRA
                     RRALDATKEAYEVASTL"
     gene            3928..4812
                     /gene="aroE"
                     /locus_tag="ACZPD3_03145"
     CDS             3928..4812
                     /gene="aroE"
                     /locus_tag="ACZPD3_03145"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR1415034.1"
                     /translation="MEQRIKGTTGLMALIGSPVGHSGSPAMYNFSFRYHNLDYAYMAF
                     DIKEDQVPAALDAIRLFKMRGANVTMPCKNEVAKHMDELSPAARIIGAVNTIVNEDGK
                     LVGHITDGIGFVRNLKEHGVDVKGKKMVVLGAGGAATAIQVQCALDGAESISIFNPKD
                     PFFARAESTAEKLSKETPDCKVSVFDLEDEAKLKEEVANADILVNATLAGMKPHEELT
                     LIKDKSMFRPDLVVADVVYNPAETRMVKEAKEAGCKLAIGGKGMLLWQGAAAYKLYTG
                     LEMPTAEYQKFQEENENK"
     gene            4833..6032
                     /locus_tag="ACZPD3_03150"
     CDS             4833..6032
                     /locus_tag="ACZPD3_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1415035.1"
                     /translation="MKQKTNKMLIFTSLAAYFTYFIHGIGASILGQYKPEFAAAWGAK
                     PLADGTLDVSMVVSVIAALGLGRLISLPFSGPLSDKYGRKLSGIIGVLCYVAYFFGMA
                     NSTSMAMGYAFAVIGGIANSFLDTCVSPTCMEIYVNNPSVANLFTKFSICLSQFLLPF
                     LIGIVASANMSYKTIFIVAGIAILIDGILILILPFPAREKAVQAKADKKKSGHKISPA
                     AIAAILIGFTSSSTFMLWLNCNQELARSYGMADPSKIQSLYALGTATAILATAAFIKK
                     GLKEINVLILYPLISTIMLALCYFIQAPFICLVGGFVIGYAGAGGVLQLAVSTTAEFF
                     PENKGTATSLVMIASSIANYTILSLAGYITKVGGSSAPRMILLLNMAVTIIGILLALF
                     VKKNRNQ"
     gene            6311..>7039
                     /locus_tag="ACZPD3_03155"
     CDS             6311..>7039
                     /locus_tag="ACZPD3_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415036.1"
                     /translation="MAYTRTQRRENELPYIPFGPFQIRLPFIHYKIESVEFIQGLILG
                     VTALAAVPYLEQYLGLPYELAWSCVIIETFLYLLHSLLGDPVVPGWITPTLPLTIVFL
                     EGFPMGKERIQAMIALQMLVGLVFIFMGVTRLADKFVHAVPNSVKGGILLAAPVTVMA
                     GQLGENGNMHKYPLAIVAGVGLLILISFSDKYQEKRKTNKFLDLVARYGNLFPYLIAM
                     VVGLLVGELDAPGLEIGTFIKIPQF"
BASE COUNT         2193 a         1460 c         1609 g         1777 t
ORIGIN      
        1 ctgtctggca acaggtataa attcaatcag tggttcatta tcaatcatag agcagaaacc
       61 cagatcatat tttttctctt ttaaaccatt taaaatatca tctgttaaaa ctttgtcaca
      121 atacagatta aaatcaatct ttttcgttgg atttgccgcg agaaatcctc tcatgatctt
      181 cgggagaaag tcaattccca gagtgcgcag gaatgcaaca tcaatacagc cctctccctt
      241 acctgccagc ttcagactgt tcacagaaga gtccagacgg ttcagtacct cttccacgtc
      301 attcaggaaa acttttccat acttggtaag agtaacattc cttccatttt tttcaaacag
      361 tttcacgccc agttcttcct caagagtgga gatcgcataa ctcagcgaag gctgggtgat
      421 cgccagaata tcagcggctt ttgtgtaatg ttcaatatga gccagcgtag aaaagtaccg
      481 cagatgatac aggttcatag aaaaactcct ttctctgata aatattttat aagtgactat
      541 attttatgtg caggctttca tagtccttta atgcttcgga aaactataat tatagtcact
      601 cttgcacaaa taatccgcgc tgtatttgtc atatacgtag attatatcat gatgtattga
      661 aaaaatctat gaatttaaag gatagaacta atttgtttta tatttaacaa agatatataa
      721 tgcttatatc aaaaagatac aaaatattat caaaataaat aaaaaaatta tgcgatttta
      781 tgaggaggac ataagatgtc aaaagaattt ccaattacaa tcagttcctg gacactggga
      841 gatcagtgca aatttgaaga cagagtgatc gctgcaaaga acgcaggata cgaaggaatc
      901 ggccttcgtg cagaaactta tgtagatgcg ttgaatgagg gactttttga caaagacatt
      961 cttgctattc tggacaaaca tggtatgaaa gtaacagagg ttgagtacat cgtacagtgg
     1021 gcagaagaac acagatctta cgaacagaaa tacaaagaac agctctgctt ccatatgtgt
     1081 gaattatttg acgtaaaaca gatcaactgt ggtctgatgg aaaactactc cgtagaatat
     1141 actgctcaga aattaagaga attatgccag agagcaggaa aatacatcat cggtgttgaa
     1201 ccaatgccat acagcggaat accggatatg aaaaaaggct gggcagttgt aaaggcagct
     1261 gactgcgaga atgcaaaact gatcatggat acctggcact gggtaagagc aaatcagccg
     1321 gttgaccttt ctgtgatcga agacatcccg gcagacaaga tcgtatccat ccagatcaac
     1381 gatgtatggg aaagaccata tgcaacaaca atcttaagag acgaatccat gcatgaccgt
     1441 cttgctcccg gaacaggaat cggatgtaca gcagcgttcg ttaagatggt aaaagaaaaa
     1501 ggaatcaaac caaacgcaat cggtgtagaa gtcatcagcg atgcaatcct tgcaaaagga
     1561 cttgaatatg ctgcaaacca tacatatgag aacacaaaga aagtgcttga ggaagcatgg
     1621 ccggaggttc ttcagtaagt atctgacgga taaaccgaca gattaaagga tcaggcagaa
     1681 accttcacgc ctgcctgatc cgcattctga atgaacacaa aatagagaat gaataattaa
     1741 tagaaaagaa ttcgaaaaaa aggagaaggt cacatgaaat tcaatgcaat gtttcagccg
     1801 atcaacatcg gtccaatgac agtaaagaac cgcttcgtag ttccgccaat gggaaacaac
     1861 tttgcaaaca cagacggaag catgagtgaa cagtccgcag cttattatcg tgaaagagca
     1921 aaaggcggct tcggtctgat cacaatcgaa gcaacagtag tacataaagg cgcaaagggc
     1981 ggccctcgca agccatgtct ttatgatgac tccacaatcg aaagcttccg caaggtagta
     2041 gatgcctgtc atgcagaggg agcaaaggtt tccgtacagc ttcagaatgc aggtcctgaa
     2101 ggaaatgcaa agaacgcagg tgcaccgatc gaggcagcta caagcattcc gtcagactgt
     2161 ggaagagata caccaaagga agttacgact gaagaagttt atgaactggt aaaaggctat
     2221 ggcctcgcag caaagagagc catggaagca ggcgtagatg cagttgagat ccatatggca
     2281 cacggctatc tggtaagcac cttcctttct ccgagaacaa acaagagact ggacgaattt
     2341 ggtggttcct ttgagaacag aatgcgtttc tcccgtctga tcatcgaaga agttaagaag
     2401 atgacagaag gaaagatcgc agttcttgca agaatcaaca gctgtgatga agtaccgggc
     2461 ggtcttgatg tgcatgacag tgctgcgatc gctgcatatc tggaaggatg cggactggat
     2521 gccatccatg tatccagagc tgttcacatc cgcgatgaat ttatgtgggc accgacagtt
     2581 acacacggcg gtttctccgc ttctcaggta gaagagatca aacgtgccgt atctatcccg
     2641 gtgatcacag taggacgtta tacagaaccg cagtttgcgg aattaatggt aaaagaagga
     2701 cgctgtgacc tggttgcatt cggacgtcag agccttgcag atccttatat gccattaaaa
     2761 gcgcaggaag aaagactgga ggatatgatc ccatgtatcg catgtcttca gggctgcgtg
     2821 gcaaacatgt atgcaggaaa ccctgtatgc tgtcttgtaa acccattcct tggacatgaa
     2881 gctgagggta ttgctcctgc agaaaaagct aagaaagtaa tggtaatcgg cggcggtgtt
     2941 gcaggtcttt gcgctgcatt tatcgcacag gaaaaaggtc atcaggtaac actttatgaa
     3001 gcaagtgata aacttggcgg aaacatgcgt cttgctgctt atcctccggg aaaaggcgac
     3061 atcacaaaca tgatccgcag ctatatcgta cgctgccaga aagcaggcgt aacaattaaa
     3121 atgaatcagg aagtaactct ggatcttatc agggaagaga aaccagacag cgtaattgtt
     3181 gcttccggtt ccagaacact gatccttccg atcgaaggta tcgacaatcc tgcaattatc
     3241 catggctctg atctccttga cggaaagaga gcagcaggaa agaaagtact ggttgtaggt
     3301 ggcggaatgg tgggatgcga gacagcagca ttcctcggtg agcagaacca tgatgtaaca
     3361 gtcattgagt tcagagacac tgtaggtgcc gacgtgatcc acgagcatag agtttacctg
     3421 atgaaagatt tcgaagatta caagatcaaa gagatcacag gtgcaaaggt atgcaaattc
     3481 tatgaggacg gcgtagagta cgagacagca gacggacaga gacatgaatc cagaggatat
     3541 gattctgtca tcctttccat gggattcaga aactacaacc cattcggcga agagatcaaa
     3601 gctatcgtac cggataccta tgtgattggc gatgccaccc gcgcaagaag agcacttgat
     3661 gctacgaaag aagcgtatga ggtagcttcc acattatgat gatatcaggg tcaaaagttc
     3721 aaaaagtcgt tcgtgcttgc acgataagat ttttgaacct gggacccgcc aaacatgaga
     3781 aagcagcgat ttacgcagta aatcagcgaa tttcgaatct tataatttat tcgttttata
     3841 gttgcagtta agagacgctg tatgatatta tcagcggaca gcagcttgaa aatgatttta
     3901 caaaaaaatt caggaggcaa acaggaaatg gaacagagaa ttaaaggaac aacaggatta
     3961 atggcactta tcggaagccc ggtaggacac tcaggatcac cggcaatgta taacttcagc
     4021 ttccgttatc acaaccttga ttatgcttac atggcgtttg acatcaagga agaccaggtt
     4081 ccggctgctc tggatgcaat ccgtcttttc aaaatgagag gcgcaaacgt aactatgcca
     4141 tgcaagaacg aagttgcaaa acacatggat gagctttcac ctgcagcaag aatcatcggt
     4201 gcagttaaca caatcgttaa tgaagacggc aaattagtag gacatatcac agatggtatc
     4261 ggatttgtcc gcaacttaaa agaacatggc gttgacgtaa aaggcaaaaa aatggtagtt
     4321 ctcggtgcag gcggtgctgc aacagcaatc caggtacagt gtgcattaga cggagcagaa
     4381 tccatttcta tcttcaatcc gaaagaccca ttctttgcac gtgcagagtc cacagcagag
     4441 aaactgagca aagaaacacc ggattgtaaa gtttctgtat ttgatctgga agatgaggca
     4501 aaactgaaag aagaggttgc aaacgcagac atcctcgtaa acgcaacact tgcaggaatg
     4561 aaacctcacg aagagcttac tctgatcaaa gataaatcca tgttccgccc tgaccttgta
     4621 gtagcagacg ttgtttacaa tcctgctgaa acaagaatgg taaaagaagc caaagaagca
     4681 ggctgcaagc ttgcaatcgg cggaaaagga atgctgttat ggcagggagc tgcagcttac
     4741 aaactctaca caggactgga aatgccaaca gctgaatacc agaaattcca ggaagaaaac
     4801 gaaaacaagt aataataacc taggagacca aaatgaaaca gaaaacaaat aaaatgctca
     4861 ttttcacctc ccttgcagca tatttcacct atttcatcca tggaatcggt gcttctatcc
     4921 tggggcagta caaaccggag tttgcagctg cctggggtgc gaagcccctg gctgatggca
     4981 ccctggatgt aagtatggta gtatctgtaa ttgcagcact tggactggga cgtctgattt
     5041 cccttccttt ctccggtcct ctgtctgata aatatggaag aaaactgagc ggaattatcg
     5101 gtgtgctctg ttatgtagca tacttctttg gaatggccaa ttccacatcc atggcaatgg
     5161 gatatgcctt tgcagtgatc ggcggaatcg cgaactcttt cctcgatacc tgcgtaagcc
     5221 ctacctgtat ggaaatctat gtaaacaacc cgtcagtagc caacctgttt acaaaatttt
     5281 ccatctgcct gagccagttc ctgcttccat tcctgattgg aatcgtagca tctgcaaata
     5341 tgtcatataa gacaatcttt attgtagcag gtattgcaat cctcattgac ggaatcctaa
     5401 ttctgatcct cccctttccg gcgagagaga aggcagtaca ggctaaagct gataagaaga
     5461 aatccggaca caagatttcc cctgcagcga ttgctgcaat tctgatcgga ttcaccagct
     5521 cctcaacctt tatgttatgg ctgaactgta atcaggaact tgcccgttcc tatggtatgg
     5581 cagacccttc aaagatccag tccctgtatg ccctgggaac tgcaactgcc atccttgcaa
     5641 cagcagcttt tatcaaaaaa ggtctgaaag agattaatgt cctgattctc tatccgttaa
     5701 tctccacaat tatgctggct ctctgctact ttatccaggc tccgtttatc tgtctggtag
     5761 gtggctttgt aatcggatat gcaggtgcag gcggtgttct ccagctggca gtttccacaa
     5821 cagcagagtt cttccctgag aataagggaa cagcaacctc actggtaatg atcgcatcca
     5881 gcattgcgaa ctacacgatc ttaagccttg caggatacat taccaaagtt ggcggaagca
     5941 gtgctccgag aatgatcctt ctcctgaaca tggcagtgac tattatcggt atcctgctcg
     6001 ctctttttgt aaagaaaaac agaaatcagt aaggcagtat gccaacaagt tgatttcctg
     6061 aataatctcg catctaaatt tcttattttc gcgttgttgt cgggtgtcgt agctaccgct
     6121 atgccttgaa ataaaaaatt attaaaatcc aatccaactg aagatatgtt tggacgttac
     6181 aagtttcaga tttttccggt gggattggat tttattaaaa tctggattgt ctaaaaatta
     6241 acaaaaaaga tagaatatat aagataaaac atagaaataa tctaataatt tattgaaaag
     6301 gagaatttgt atggcgtata caagaacaca aagaagagaa aatgaactgc cctacatccc
     6361 ctttggacca tttcagataa gacttccttt tatacactac aagatagaat ctgttgagtt
     6421 cattcagggt ctgatcctgg gcgttactgc gctggccgct gttccgtatc tggagcagta
     6481 tctggggctt ccttatgagc tggcgtggag ctgtgtaatt attgaaacct tcctttatct
     6541 gcttcacagc ctgcttggcg atccggtagt accggggtgg atcacaccga ccctgccgct
     6601 gactattgta tttctggaag gcttccccat gggaaaagag cgaatacagg ctatgatcgc
     6661 actgcagatg ctggtaggtc tggtatttat ctttatgggt gtgaccagac tggcggataa
     6721 atttgttcat gcagttccca attctgtcaa aggcggaatc cttcttgcag caccggttac
     6781 agttatggca ggacagctgg gagagaacgg aaatatgcac aaatatcctc tggctattgt
     6841 tgcaggtgta ggtcttttga tcctgatcag cttctctgac aaatatcagg agaagagaaa
     6901 aacaaataag tttcttgatc ttgttgcgag atacggaaat cttttcccat atcttatcgc
     6961 aatggtagta ggacttctgg ttggcgaact ggatgccccg ggactggaga tcggtacctt
     7021 catcaagatt cctcagttt
//
LOCUS       DBMDXG010000065         6965 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2793_length_6965_cov_1.847028, whole genome shotgun sequence.
ACCESSION   DBMDXG010000065 DBMDXG010000000
VERSION     DBMDXG010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2793_length_6965_cov_1.847028"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..782)
                     /locus_tag="ACZPD3_03160"
     CDS             complement(156..782)
                     /locus_tag="ACZPD3_03160"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR1415037.1"
                     /translation="MTREQQQRTKIKICGLKRPEDITYVNEAKPDYCGFIIEFPKSSR
                     NVTGALVRELTAKLNPDIIPVGVFVNAAPERVEELLLDGTIHIAQLHGQEDEAYIRRI
                     QKNTGHQVIKAFSVKTAQDIENALKSPADYILLDQGGGGTGQTFDWSLIPEIDRPFFL
                     AGGLGADNLETAVRTIHPYAVDLSSSVETDGMKDRDKILKAVQLVHMK"
     gene            complement(813..1613)
                     /gene="trpC"
                     /locus_tag="ACZPD3_03165"
     CDS             complement(813..1613)
                     /gene="trpC"
                     /locus_tag="ACZPD3_03165"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR1415038.1"
                     /translation="MKNILEEIAARTRERIAKEKSCISVSELENRIQEVNKNAGQKIT
                     FLQALQKDGMSYICEVKKASPSKGLIAPDFPYLAIAKEYEQAGASAISCLTEPFYFQG
                     ADQYLREISAAVQIPVLRKDFTVDEYMIYQAKSLGASAVLLICAILDDGELRAYRQLA
                     KELGLDALVEAHDEYEVDRALNLGAEIVGVNNRDLRTFQVDMNNSIRLRKMAPDNVVF
                     VSESGIRTPEDIRLLYENKVDAVLIGETLMRSPDKKAALESLNSSPLR"
     gene            complement(1713..2723)
                     /gene="trpD"
                     /locus_tag="ACZPD3_03170"
     CDS             complement(1713..2723)
                     /gene="trpD"
                     /locus_tag="ACZPD3_03170"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR1415039.1"
                     /translation="MIKEAIIKLSKKQDLAYAEAEAVMDEIMSGQATPVQMSAYLTAL
                     ALKGETIDEITASAAGMRAHCIKLLHNLDVLEIVGTGGDGSNSFNISTTSSLVIAAGG
                     VPVAKHGNRAASSKSGAADVLEALGVKITLTPERSAEILKKINICFLFAQNYHIAMKY
                     VAPIRKELGIRTVFNILGPLSNPAGANMELMGVYDQSLVEPLAQVMANLGVNRGMVVY
                     GQDSLDEISMCAPTSVCEIRDGKFTSYEITPEQFGYERCEKGALTGGTPAENAEITKA
                     ILKGEEKGPKRQAVCLNAGAALYIAGKAASIEEGVKLAESLIDSGAALKKLEEFVEET
                     NK"
     gene            complement(2716..3294)
                     /locus_tag="ACZPD3_03175"
     CDS             complement(2716..3294)
                     /locus_tag="ACZPD3_03175"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR1415040.1"
                     /translation="MILLIDNYDSFSYNLYQLIGTVEPDIKVVRNDECTLEEIAEMKP
                     EAIILSPGPGRPEDAGICIPMIKEFAGKIPILGVCLGHQSICEAFGGIVSYAKELMHG
                     KKKLMYKTGESQLFKGLPDTFAAARYHSLAALKDTLPAELKVTAESEDGEVMAVEHTK
                     YPVFGVQFHPESVMTPDGRVMIENFMEVVRND"
     gene            complement(3291..4778)
                     /gene="trpE"
                     /locus_tag="ACZPD3_03180"
     CDS             complement(3291..4778)
                     /gene="trpE"
                     /locus_tag="ACZPD3_03180"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR1415041.1"
                     /translation="MYPTLETIRQLAQTKEYRRIPLCRELYADRYTPVEVMRILRKAS
                     RHCYLLESASQTEVWGRYSFLGYEPSMEITCTDRTLKIRTTDAEGKTEETVRQVTHPG
                     DTLREIIRKYKTPVMEKMPPFTGGLVGYFSYDYIKYSEPKLKLEDREQQDFRDMDLML
                     FDQVIVFDHFRQKVLLITGVMADDLEASYEKAVKKLEEMAQLIRNGEHMEFEPLRLQS
                     EIQPKFPKEKYAEMVEKAKHYIREGDIFQVVLSNPMRAKATGSLFDTYRVLRASNPSP
                     YMFYFSSDDIELAGASPETLAKLENGTLSTFPLAGTRPRGKTREEDKELEEGLLKDEK
                     ELAEHNMLVDLGRNDIGKISKIGIVKVEKYLCVERFSHVMHLGSTVTGIIRDDKDAVD
                     AVDAILPAGTLSGAPKFRACQIIEELEQSKRGIYGGAIGYLDFAGNLDTCIAIRLVYK
                     KNGEICIRSGAGIVADSVPEKEFQECCNKARAVVQAIEQAQEGLE"
     gene            complement(5406..6866)
                     /locus_tag="ACZPD3_03185"
     CDS             complement(5406..6866)
                     /locus_tag="ACZPD3_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR1415042.1"
                     /translation="MEKQEKNTVTPYDLDGRPPLKLAIPLGLQHVLAMFVGNLTPLLI
                     ITAACGIEAGGELQVALLQNAMLIAGIVTLVQVFTIGPVGGKLPIVMGTSSGFIGVCQ
                     SVAGVMGNGVVAYGAIMAASFIGGLFETVLGSFLKPLRKFFPSVVTGTVVLSIGLSLI
                     AVGISSFGGGSSAKDYGSLENLFVGFVVLVVIVVLKHGTKGFTSFASILIGIIVGYVL
                     VSIMAAVLPTTFTYVDDAGATVEATKSWVLNWDKVASASWFAVPKIMPVKWVFDARAI
                     VPICIMFIVTAVETVGDISGITEGGLGREATDKELSGGVMCDGLGSSLAAVFGVLPNT
                     SFSQNVGLVAMNKVVNRFSIATGGIFLIACGLFPKLAALISIMPQSVLGGAAVMMFSS
                     IVVSGIQLITKWPVTPRNVTIVSVALGLGYGLGANSAVLAHMPQAITLIFGGSGIVPA
                     ALFAIILNIVLPPDEKSEVEIAEEILDMKKKEAQKK"
BASE COUNT         1702 a         1794 c         1341 g         2128 t
ORIGIN      
        1 gatattcgca atccgcttcg ctacttgctc ataaccgaat aactgctccg cagtttatca
       61 gaaggagctt gcactcctcc tgatacaagc aagtccccac ccactgctta ttgcttttcg
      121 caattgaagc aggtgactta cttgattcgg ttatcttatt tcatatgcac cagttgaact
      181 gccttcaaaa tcttatctct gtctttcatt ccatccgtct ccacactact gctcagatct
      241 actgcataag gatgaatagt ccggacggct gtttccagat tatctgcccc cagtcctcct
      301 gccaggaaaa acggtctgtc tatctccggg atcagtgacc agtcaaaggt ctgtccggtt
      361 ccaccgccac cctgatcaag cagtatataa tctgccggac ttttcagggc attttcaata
      421 tcctgagctg tttttacgga aaaggccttt ataacctgat gacctgtatt cttttggatt
      481 ctccgtatat atgcctcatc ttcctgtcca tgaagctgtg caatatggat agttccgtca
      541 agaagcagtt cctccacacg ctcaggtgct gcatttacaa aaacacctac agggataatg
      601 tccggattaa gttttgcagt aagttctctc accaatgcac ctgtaacatt tctgctgctc
      661 ttcggaaatt ctatgataaa tccgcagtaa tcaggttttg cttcatttac ataggtaatg
      721 tcttccgggc gtttcagacc acagattttg attttggttc tttgctgttg ttctctggtc
      781 atattctatc ctctcagata acattcgatc tttcagcgta acgggctgct attcaggctt
      841 tccagcgctg ctttcttgtc cggacttcgc atcagtgttt ccccgatcag taccgcatcc
      901 actttattct cataaagaag ccggatatcc tccggggtgc ggattccgct ctcggaaaca
      961 aaaaccacat tgtccggtgc catttttctc aggcgaatgc tgttgttcat atctacctga
     1021 aaagttctca gatcccggtt attgactccc acgatctctg ccccgagatt taaggctctg
     1081 tccacttcgt actcatcgtg tgcttctacc agtgcatcca gtcccagctc ttttgcaagc
     1141 tgtctgtatg ctcttaattc tccgtcatcc aggatggcgc agataagcaa taccgcggaa
     1201 gcaccaaggg atttcgcctg atagatcata tactcatcta ctgtaaaatc cttgcggagt
     1261 acaggtatct gtacagcggc tgagatttct ctcagatact ggtctgctcc ctggaaataa
     1321 aaaggttctg tgagacagga gattgcactt gcacctgcct gttcatattc ctttgcaatt
     1381 gccagatatg gaaaatccgg tgcaatcagt ccctttgagg gggaggcttt ctttacctcg
     1441 cagatatagg acataccgtc tttttgtaat gcctggagaa atgtgatttt ctgccctgca
     1501 tttttattta cttcctgaat ccggttctcc agctccgaaa cagaaataca ggatttttct
     1561 tttgcaattc tttctcttgt tctggctgct atttcttcta atatattctt cataatctct
     1621 atgatttttc tgtaactgtt ctgtatatcc acagtcagca gattaccgga cagttacaga
     1681 tttcctttac attagtaatt gggggttgct ttttatttat ttgtctcttc tacaaattct
     1741 tccagttttt tcagtgctgc accgctgtcg atgagggatt ctgccagctt cactccttct
     1801 tctatggaag cggcttttcc tgcaatatag agtgctgcac ctgcatttaa acataccgcc
     1861 tgacgcttcg gacctttttc ctcacctttc aggattgctt ttgtgatctc tgcgttttct
     1921 gcaggtgtac cacctgtcag ggcacccttc tcacatctct catatccaaa ctgttccggt
     1981 gtgatctcat aggaagtaaa tttgccatcc ctgatctcac atacagaagt aggtgcgcac
     2041 atggaaatct catccagact gtcctgtcca tagacaacca ttcctctgtt aacgcccaga
     2101 tttgccataa cctgtgccag tggctccacc agtgactgat cgtaaactcc catcagttcc
     2161 atgtttgcac ctgccggatt acttaacggt ccaagaatgt taaatacagt acggattcct
     2221 agctctttac gaatcggtgc cacatatttc attgcaatgt gataattctg cgcaaacagg
     2281 aagcagatgt tgatcttttt caggatctct gcactgcgtt ccggcgtcag tgtaatcttc
     2341 actcccagtg cttccagaac atctgctgca ccggatttgg aggatgctgc cctgtttcca
     2401 tgttttgcaa ccggaacacc gcctgctgcg atcaccagag aagaagtagt agaaatattg
     2461 aaggaattag aaccgtctcc gccggtaccc acgatttcaa gcacatccag gttgtggaga
     2521 agtttgatac agtgagctct cattcctgct gcggaagctg tgatctcatc aattgtctct
     2581 ccttttaaag ctaatgcagt aagataagca gacatctgta caggtgttgc ctgaccgctc
     2641 ataatctcgt ccattacagc ttctgcttcc gcataagcta aatcctgttt ctttgataat
     2701 ttaataatgg cttccttaat catttctcac tacctccata aagttctcta tcatcactct
     2761 accgtccggt gtcattaccg attcaggatg aaactgtact ccgaacacag gatattttgt
     2821 atgttccact gccatgacct ctccgtcctc tgactctgct gtcaccttaa gttctgcagg
     2881 cagggtatcc tttaacgctg ccagggaatg atatctggct gctgcaaagg tatccggcag
     2941 acccttaaaa agctgacttt ctcctgtttt atacatcagt tttttctttc catgcatcag
     3001 ctcttttgca taggaaacta ttcctccaaa ggcttcgcag atggactgat gtcccaggca
     3061 cacacccagg atggggattt ttcctgcaaa ttctttaatc atcggaatac agatacctgc
     3121 atcctccggt cttcccggac caggagataa aatgatggct tccggtttca tctctgcgat
     3181 ttcttccagt gtacactcat cgtttctcac tactttgatg tccggttcca ctgtgccgat
     3241 gagctgatat aagttatagg aaaagctgtc ataattatcg attagtaata tcattctaat
     3301 ccctcctgtg cctgttcaat tgcctgtacc actgctcttg ccttgttgca gcattcctgg
     3361 aattcctttt ccggtacact gtctgccacg attccggcac ctgaacggat acagatttct
     3421 ccgtttttct tgtaaaccag gcggatggca atacaggtat ccaggtttcc tgcaaaatcc
     3481 agatatccga tggcgcctcc gtaaatgcct cgtttgctct gttccagttc ctcgatgatc
     3541 tggcaggctc tgaatttggg tgctccggaa agggttcctg ccggaaggat tgcgtccact
     3601 gcatccacag catctttatc atcccggata atccctgtta ctgtagagcc cagatgcatt
     3661 acatgggaaa aacgttctac gcagagatac ttttctactt ttacaattcc gatcttgctg
     3721 atctttccaa tatcgtttct tcccaggtct accagcatat tgtgttctgc cagctctttc
     3781 tcatctttca gaagtccctc ttcaagttct ttatcctctt ctcttgtctt tcctcttggt
     3841 ctggttcctg ccagagggaa ggtacttaag gtgccatttt ccagcttcgc aagggtttcc
     3901 ggggaagcac ctgccagttc aatgtcatca ctggagaaat aaaacatata aggagaagga
     3961 ttagatgccc gaagaacccg gtaagtatca aacaggcttc ctgttgcttt cgcacgcatg
     4021 ggattggaaa gtactacctg gaaaatatca ccttctctga tgtaatgttt ggctttctcc
     4081 accatctctg catatttttc tttcggaaat ttcggctgta tttctgactg aagtctcagc
     4141 ggttcaaatt ccatatgctc cccgtttcgg atcagctgcg ccatttcctc caatttcttt
     4201 actgctttct cgtaggaggc ttccagatca tctgccatca cacctgtgat cagaagtact
     4261 ttctgtctga aatggtcaaa tacgatcacc tgatcaaaaa gcattaaatc catgtccctg
     4321 aaatcctgct gttccctgtc ctccagtttc agctttggtt cactgtattt gatataatcg
     4381 taggagaaat atcctaccag accgcctgta aaaggaggca tcttttccat taccggagtt
     4441 ttatatttcc ggatgatttc tctgagagta tcaccgggat gtgtgacctg ccttacggtt
     4501 tcctctgttt ttccctcagc atcggttgtc cggatcttca atgttctgtc tgtacaggta
     4561 atctccatgg acggttcata ccccagaaaa gaatacctgc cccacacttc tgtctggctg
     4621 gcactttcca gcaggtaaca atggcggctg gcttttcgca aaatgcgcat tacctctacc
     4681 ggtgtatatc tgtctgcata aagttctctg cacagaggaa tacgacgata ttcttttgtc
     4741 tgtgcaagct gcctgatggt ttctaatgtt ggatacatgg aaacacctcc tgtgattaat
     4801 tcagtttctg ccaagaacat aaaaaagccc gcccttgaca gaattatcgt ttctgtcaag
     4861 gacgagcgta tatacttcac ccgcggtgcc accttgattt acggtttgaa ccgtactctt
     4921 tgcgaaatac cttcatattc ccggcaactg acgtatgcct cacgtcacag gatacttagt
     4981 ctgctctgta atcttcaacc tttcactgtg ccctcagcgg cccatttggc agactgcatt
     5041 tccacccggc tctcaccttc ccggattctc tgtgcgcgca tgactgcttt gacttccgct
     5101 tcatcggttt aatctgttga atttacactt aatttaccac cggaatctat atttgtcaac
     5161 cctgaatttc attttcaata acatattttt atcattacgt tgttgttcac tccgttctca
     5221 gtaacgtagc caaaattcat tccagacatc tgcagtctgt tttcgccaca tacatctgca
     5281 ccagataatc attcactata atctgaataa aagcgcaaaa ccagatattc ccagtttcgc
     5341 gctttttatt gttttactta ttcactttcg aacaatccca gtgtgccaac aagttgattc
     5401 tgctgctatt tcttctgtgc ctcttttttc ttcatgtcaa gaatctcttc tgcaatctca
     5461 acttcggatt tctcatccgg tggaagcacg atattcagaa tgatagcaaa cagtgctgca
     5521 ggtacgattc ctgaaccgcc aaagatcaat gtgattgcct gcggcatatg tgcaaggact
     5581 gctgagtttg ccccaagtcc atagccaaga ccaagtgcaa cggagacaat tgttacgttt
     5641 ctcggagtta ccggccattt tgtgataagc tggataccgc tgacaacaat agatgagaac
     5701 atcataactg ccgcaccgcc cagtacactc tgaggcatga tggagatcag tgctgcaagc
     5761 ttaggaaaca gaccacatgc gataaggaaa ataccgcctg ttgcaattga aaaacggttt
     5821 acaaccttgt tcattgcaac cagacctacg ttctggctaa aagaagtatt cggaagtacg
     5881 ccaaatactg ctgccagtga agaaccaaga ccatcgcaca taacgccgcc agaaagttct
     5941 ttgtctgtag cttctcttcc aagaccgccc tctgtgatac cggaaatatc acctacagtc
     6001 tctacagctg ttacaataaa catgatgcag atcggaacga ttgcccttgc atcaaatacc
     6061 catttgacag gcatgatctt cggcactgca aaccaggatg cgcttgctac tttatcccag
     6121 ttaagaaccc aggatttggt agcttctact gttgcacctg catcatccac ataagtaaat
     6181 gtggtaggaa gaactgctgc cataatactt accagcacat atcctacgat aataccgatc
     6241 agaattgatg caaagcttgt aaatcctttt gttccatgtt ttaatacgac aattacaacc
     6301 agaactacaa aacctacaaa cagattctcc agagaaccgt agtccttagc ggaacttccg
     6361 cctccaaaag aactgatacc aacagcgatc agtgaaagac cgattgataa tacaacggtt
     6421 cctgttacaa ctgacggaaa aaattttctc agtggtttca ggaaacttcc aagtacagtt
     6481 tcaaaaagac cgccgataaa ggatgccgcc atgatcgcac catacgcaac cactccatta
     6541 cccatgactc ctgcaacact ctggcatacg ccaatgaaac cggaactggt tcccataacg
     6601 atggggagtt taccacccac aggtcctatg gtaaatacct gaaccaaagt tacaatgcct
     6661 gcaatcagca tggcattctg cagaagtgct acctgcagtt ctccgccggc ttctatccca
     6721 caggctgctg tgatgatcag aagcggtgta agattaccta cgaacatagc cagcacatgc
     6781 tgcaaaccaa gaggaatcgc cagcttcagt ggcggccttc catcaaggtc gtagggtgtt
     6841 actgtgtttt tctcttgttt ctccattcct ttccctctct ttcgtgaatg ttgatgtgat
     6901 tcggcaaaag gttttacaat agcgattttt cctataatca aaaacaaggc agttatcctt
     6961 ttttg
//
LOCUS       DBMDXG010000066         6862 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2840_length_6862_cov_1.988315, whole genome shotgun sequence.
ACCESSION   DBMDXG010000066 DBMDXG010000000
VERSION     DBMDXG010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2840_length_6862_cov_1.988315"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /gene="pglX"
                     /locus_tag="ACZPD3_03190"
     CDS             complement(<1..1896)
                     /gene="pglX"
                     /locus_tag="ACZPD3_03190"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HIR1415043.1"
                     /translation="MDKNAIKKFAVWARTELIARVSLKGVEYGITEDNIEAANADSVG
                     GKVLTADEKKQRQALIAEINDKGYKQVMEEVAYTWFNRFSALRFMEVNGYLPSHVRVF
                     TDEENNFKPQIITEAIHLDLDGLDMEKVYELKDAEKTEELYKYLLIVQCNALNKILPG
                     MFQKIADYTELLLPDNLLREGSVIQQMIELIPEDDWKDAVQIIGWLYQYYNSEKKDDV
                     FAALKKNVKITKENIPAATQLFTPDWIVRYMVENSLGRLWLEGHPEASEQLLPTEEEQ
                     SAYAAGNRDPEDAKWHYYLEEAEQEPEVQAQLAEIRKEYAALTPDQLKVIDPCSGSGH
                     ILAYMFDVLMKIYESYGYTTREAVASIVENNLYGLDIDDRAAQLAYFAVMMKARQYDR
                     RFFSRGIQPHVYAIVESNHVDKFVLDYFCNGDMKLTAAMDTIISELHDAKEYGSILTV
                     TQQDWFTLYNRFDEIIEDINLSREMALRELLPLVQVAETLAQAYDTVVTNPPYMGSNN
                     MGEKLNGYVKNNYPDTKSDMSTVCMEKTLKMCNSNGMMAMINIPVWMFLTSYEKLRRK
                     MISENTFLNMLHLGRGVFGSDFGTTAFVICKRYLRNHIGNYRRLFNKSVEVVTVETKE
                     KWFFEMKG"
     gene            complement(1912..5460)
                     /gene="brxC"
                     /locus_tag="ACZPD3_03195"
     CDS             complement(1912..5460)
                     /gene="brxC"
                     /locus_tag="ACZPD3_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HIR1415044.1"
                     /translation="MMIRDMFADDINRKINGVIKVDQAADDVIEQELNEYVITRELKK
                     HFITFFNYYGDAFDQPTADMGVWISGFFGSGKSHFLKMLSYLLENKEVKGIRSVERFR
                     KKFEDDPATFMLIDRATKGQTETILFNIDIEGFSNKDKTAVLRVFAKMFYNHLGFYGE
                     NLKVAMMERYIDQQGKTEEFRRVFEEKKGKSWMEMRRAFAFNGKFIIPTLMEVLDMSE
                     DDAKAWFNDKTATEISIAQLVEDMKAYVDTKPANFRLLFMIDEVGQYVGTDTDMLLNL
                     QSLTEKIGSECEGKIWVICTGQEAIDEIIKVRADEFSRIQARFKTRLSLSSSSVDEVI
                     QKRILKKKPEATMDLETVYEQNDSVLRNLFSFNGSILDIKGYSGPREFTENFPFVPYQ
                     FIIMQKVFAEIRKHGNSGKHLSGGERSMLSGFQEAAQKIQEKDEYALVPFFRFYDTVH
                     TFLDGSIRRVIERCQKAADNGDGIEQQDVDVLKLLYLIRYIDDIPSNLDNIVILMADD
                     IRVDKIIMREAVRGCLDRLMSQNYIGRTGDTYNFLTDEEQDIQREIRDTNVDTASIVE
                     RIAQMIYGDIFTTKKFRYGKYDFAFDQMVDGITVGVATGGMRLRFLTVATDAIEKTDY
                     RLMAESKGNEAIVVLADTPYYESLESAMKIRKYVKQRNVSQLPKTVQKIISDQQDEAG
                     KYELSAMSELQNAIEGAQFYVDGEHLEIRAGNAKSKIDQSLEYLVAHVYSKLDLITDN
                     AGSDVDIIAILTGAVTALPGMEPNRDAASAMEEYLEMQDVKKLPTSMADVQSKYSAIP
                     YGWKEIDIAAVAAQLIYSQKVTIKYAGNTIQPDDPKLPDMLRKKSEIGKTSISKRKNI
                     SATMMRDVKAMLREYFDIMDVPDDEDGLIRFVTEKFTEQRDYYASLDACYDGHKYPDR
                     ALVQEAIHLMDDVLSQKKDNIALIERVLKKEDALFNNKEAMSNGIENFFKTQVTVFDQ
                     AVQFEKSLHDDLDRIAENEEAHKALNTIRLITMVQTGSKFNYNRIRELNPLMDTVRTA
                     HDKMLEEKRAEVLETVRQCMEATHTAANGDSKASHLIEKSDRYFSQCKEKIAELKSLA
                     LLDAMFLPMCQYKDDTISNIESVLAPPAPKPQVQPTQPGKETAPAKKKVVRAYNRQVV
                     FQAKTLQTDEDIDDYVEKIRSQLKQLLKNCDEIKLN"
     gene            complement(5474..6034)
                     /locus_tag="ACZPD3_03200"
     CDS             complement(5474..6034)
                     /locus_tag="ACZPD3_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HIR1415045.1"
                     /translation="MSDIKERLDKVRALIQEPEFLEGKGLSNEVNIRIFCYEPENEMV
                     VRHFVNQLETDQSLDCHLIVCNLYKTFLSICDDMDITDAIPDMEEADGSAYLLEQLNS
                     AIGNGEFIDKIQYEPHEPGDVLMLTGVGEVFPFMRIHTLLEALQPYFSDVPILVMYPG
                     EFDGRHVKLFNRLTPNDYYRAFNVID"
     gene            complement(6045..6650)
                     /locus_tag="ACZPD3_03205"
     CDS             complement(6045..6650)
                     /locus_tag="ACZPD3_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HIR1415046.1"
                     /translation="MDHSYPYIASLTREPFLFYEMRSTAKLMAEGNSDDAIVKEIVEQ
                     NLFQYPTEKSITRMAKACIKRLQALEDDSLVSAIASQPTDVAKQICLYALMKQSRLVW
                     EFMLTVIGEKYRLRDTSFGKIDLNTFFMRLQEQNDTVASWSDTTITKLKQIIARVLVE
                     TEYLDNRGADHLNPVWLHPVLENAIRSNGDMAILPAFNCFS"
     gene            complement(6665..>6862)
                     /locus_tag="ACZPD3_03210"
     CDS             complement(6665..>6862)
                     /locus_tag="ACZPD3_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415047.1"
                     /translation="LWKLLIDKEMMKKDLRAMTGVSTTTMSRLSKDENVSTEILSKIC
                     SALNCDVGDIMEFVPDKKEEQ"
BASE COUNT         1848 a         1711 c         1254 g         2049 t
ORIGIN      
        1 acctttcatt tcgaaaaacc atttttcttt tgtttcaaca gttacaactt caactgactt
       61 attaaaaagt cttctataat ttccaatatg atttcttaaa tatcttttac aaataacaaa
      121 tgcagtagtt ccaaaatcag atccaaatac tccccttcct aaatgtaaca tattcaaaaa
      181 agtattttcg gaaatcatct ttctacgcaa tttctcatag ctagtcaaaa acatccatac
      241 cggtatattt atcatcgcca tcatgccatt tgaattacac atttttaacg ttttttccat
      301 gcataccgtg ctcatatctg atttggtatc tggatagtta tttttcacat aaccatttaa
      361 tttttccccc atattattag atcccatata tggcggattc gtcacaaccg tatcatatgc
      421 ctgtgctagc gtttcagcca cttgtaccaa cggcaacaat tctctcaatg ccatctcacg
      481 ggataggttt atatcttcta taatctcatc aaagcggtta tacaaagtaa accaatcctg
      541 ctgtgttaca gtcaaaatcg aaccatattc ctttgcatca tgcagttcac tgataatggt
      601 atccatagcc gctgtgagct tcatatctcc attgcagaaa taatccaaaa cgaatttatc
      661 tacatgatta ctctccacaa tagcatatac atggggctga atgccacgac taaaaaaacg
      721 gcgatcatac tgacgggctt tcatcattac cgcaaaataa gccaactgag ccgcacgatc
      781 atcaatatcc agcccataaa gattattttc tacaatactt gccaccgctt cacgggtggt
      841 gtagccataa gattcataaa tcttcatcag cacatcaaac atatacgcaa gaatatgacc
      901 ggaaccggag catgggtcaa tgactttcag ctggtccggt gtcagtgcgg catattcctt
      961 acggatttca gccagctgtg cctgtacttc cggctcctgc tcggcttctt ccagatagta
     1021 gtgccatttg gcatcttctg ggtcacggtt tccggcagca taagcagact gttcttcctc
     1081 tgtcggaaga agctgctctg atgcctccgg gtgtccctcc agccacaaac gaccaaggct
     1141 gttttctacc atgtagcgaa caatccagtc cggtgtgaaa agctgggttg ctgcagggat
     1201 gttttctttt gtgattttca cattcttttt cagtgctgcg aaaacgtcat cctttttctc
     1261 actgttataa tactgataca accagccgat gatctggaca gcatctttcc agtcatcctc
     1321 cggtatcagc tcgatcatct gctgaatgac actgccttca cggagcagat tgtccggcag
     1381 aagaagctcg gtgtaatccg caatcttctg gaacattccc ggcaaaatct tattcagagc
     1441 gttgcactgc acgatcagca gatatttata cagttcttcg gttttttcgg catcttttag
     1501 ctcatagact ttttccatat ccaatccatc caaatccaga tgaattgctt cggttataat
     1561 ctgcggctta aagttgtttt cctcgtccgt gaatacacgc acatgggaag gaagatagcc
     1621 gttgacttcc ataaatcgca gagcagagaa gcgattgaac caagtgtaag caacctcctc
     1681 catgacctgc ttatagcctt tatcgttaat ctcggcaatc aaagcctgac gttgtttctt
     1741 ttcatcagca gtgaggactt tgccgccaac gctgtccgca ttggcggcct cgatattatc
     1801 ctctgtgata ccgtattcca cgccttttag ggaaacacgg gcgatcagct ctgttcttgc
     1861 ccagacagca aattttttaa tcgcattctt atccatactc gtctctcctc gttagttcag
     1921 cttgatctca tcgcaatttt tcaagagttg cttcagctga gaacgaatct tctctacata
     1981 atcatcaatg tcttcatccg tttgtaaggt ctttgcctgg aatacaacct gtcggttata
     2041 agcacggaca acctttttct ttgccggagc agtttctttt cctggctgtg tcggctgtac
     2101 ctgcggcttc ggtgcaggcg gagccagaac tgactcgata ttgctgatag tatcatcctt
     2161 gtactggcac atcggcaaga acatagcatc cagaagggca aggctcttta attctgcgat
     2221 tttctccttg cactgactga aataccgatc cgatttttca atcagatggg atgccttgga
     2281 atcgccgttt gcagcagtat gggtcgcttc catacactga cgaaccgttt ccagaacctc
     2341 agcacgtttt tcttccagca tcttgtcatg ggcggtgcga accgtatcca tcaacggatt
     2401 cagttcacga atccggttat agttgaactt gcttccggtc tgaaccatcg taatcaatcg
     2461 aatagtattc agtgccttat gtgcttcctc attttctgca atccggtcaa ggtcatcatg
     2521 cagagatttt tcaaactgca ctgcctggtc gaatactgtt acctgggttt tgaagaagtt
     2581 ttcaatgccg ttgctcatgg cttctttatt gttgaaaaga gcatcctctt ttttcagcac
     2641 acgctcaatc agggcaatat tgtctttctt ctgagaaaga acatcatcca tcagatgaat
     2701 agcttcctgc actaatgcac gatccgggta tttatgtccg tcataacaag catccaaaga
     2761 agcatagtaa tcacgctgct cggtaaactt ctccgtcaca aagcgaatca gaccatcttc
     2821 atcatccggc acatccataa tgtcgaaata ctctcggagc atcgctttta catcacgcat
     2881 catcgtagca gaaatgtttt tacgcttgct gatggaagtc ttgccgatct cactcttttt
     2941 gcggagcata tccggcaact tcgggtcatc cggctgaatg gtattgcctg catacttgat
     3001 ggttactttc tgggaataaa tcagttgtgc cgcaaccgca gcaatatcga tttctttcca
     3061 tccatacgga atcgcactgt atttgctttg cacatctgcc atagaagtcg gcaacttttt
     3121 cacatcctgc atttccagat attcttccat agcagaagca gcatcacggt tcggttccat
     3181 gcccggaagt gccgttaccg cacctgtcag aatcgcaata atatctacat cactgcctgc
     3241 attatctgta atcaggtcga gcttgctata tacatgggca accagatatt caagcgactg
     3301 gtcaatcttg ctcttagcat ttccagctct gatttccaga tgctcaccgt ctacatagaa
     3361 ctgcgcccct tcaatagcat tttgcaattc gctcatggca ctcagctcat attttccggc
     3421 ttcgtcttgc tggtcactga taattttctg cacagttttt ggaagctggc ttacatttct
     3481 ctgctttacg tacttacgaa tcttcatagc agattccaga gattcatagt aaggcgtatc
     3541 agccagaacc acgattgctt cattaccttt ggactccgcc atcagacggt aatctgtttt
     3601 ttcaatagca tcggtagcaa cggtcaggaa acgcagacgc ataccacctg ttgccactcc
     3661 aactgtaatg ccgtccacca tctgatcgaa agcaaagtca tattttccat aacggaactt
     3721 cttggttgtg aaaatatcac cgtaaatcat ctgagcgata cgctctacga tagaagcggt
     3781 atctacattg gtatcccgga tttcacgctg aatatcctgt tcttcatccg tcaggaagtt
     3841 ataagtgtct ccggttctgc cgatatagtt ctggctcatc agacggtcaa ggcatccacg
     3901 gacagcttca cgcatgataa tcttatccac acgaatatcg tccgccataa ggataacgat
     3961 attgtccaga ttagaaggaa tatcatcaat atatcgaatc aagtacagaa gtttcagcac
     4021 atccacatcc tgctgttcaa tgccatcacc gttgtcggca gctttttggc aacgttcaat
     4081 tacacgacga attgaaccat ccagaaaagt atgtacggta tcataaaaac ggaagaacgg
     4141 aacaagtgca tactcatcct tctcctgaat tttctgtgca gcttcctgga aaccggacag
     4201 catggaacgc tcaccaccgg aaaggtgctt gccggaattt ccgtgcttgc ggatttcagc
     4261 aaataccttc tgcataataa taaactgata cggcacgaac gggaagttct cagtaaattc
     4321 cctcggaccg gaatagcctt taatatccag aatagagcca ttgaagctga acaggttacg
     4381 caggacagaa tcattctgct cataaacggt ttccaaatcc attgttgctt ccggtttctt
     4441 tttcagaata cgcttctgga tgacttcatc cacggaggaa gaagataagc tcagtcttgt
     4501 tttgaaacga gcctggatac gggagaactc atcggcacga actttgatga tttcatcaat
     4561 ggcttcctgt ccggtgcaga tcacccaaat ctttccttca cactcacttc cgattttttc
     4621 tgtcaaagac tggaggttca aaagcatatc ggtgtctgtg ccgacatact gaccaacttc
     4681 gtcgatcata aacagcagac ggaagttcgc tggtttagta tccacatagg ctttcatatc
     4741 ttccacaagc tgagcaatag atatttctgt tgccgtttta tcattgaacc atgccttggc
     4801 atcgtcctca ctcatatcca gaacttccat taaagtcgga ataataaact ttccattgaa
     4861 tgcaaaggca cggcgcattt ccatccagga cttgcctttc ttttcttcaa aaactctgcg
     4921 aaactcctca gtttttccct gctggtcaat ataacgctcc atcatggcaa ctttcagatt
     4981 ctcaccgtaa aatcccagat ggttgtagaa cattttggca aatacacgaa gaaccgcagt
     5041 cttatcttta ttgctgaatc cctcaatatc aatattgaac agaattgtct cggtctgtcc
     5101 ctttgtggca cgatcaatca gcataaaggt tgccgggtcg tcctcaaact ttttacggaa
     5161 acgctccaca ctgcgaatgc cttttacctc tttattttcc aaaaggtagg aaagcatttt
     5221 caggaagtga gatttaccac ttccaaagaa accggaaatc caaacaccca tatcggctgt
     5281 cggctggtca aaagcatcac cgtaataatt gaagaacgta atgaaatgtt tcttcaattc
     5341 tctggtaatg acatattcat ttaactcctg ctcgattaca tcatcagcag cctgatctac
     5401 tttaattaca ccattgattt tgcggttaat gtcatccgca aacatatctc gaatcatcat
     5461 ggctttatcc cccttaatct atgacattga atgcccggta ataatcattt ggtgtcagac
     5521 gattaaacag ttttacatga cgaccatcaa actcacccgg atacataacc aaaatcggaa
     5581 catccgaaaa atacggttgc aaggcttcca ataacgtatg gattctcata aacgggaaca
     5641 cctcgcccac acctgtcagc atcagcacat cacccggttc gtgtggctca tactggattt
     5701 tatcaatgaa ctctccattg ccgattgcgg agttaagctg ctccagaaga taagcacttc
     5761 cgtcagcttc ttccatatcg ggaattgcat ctgtgatgtc catatcatcg cagatagaga
     5821 gaaatgtttt atataaatta caaacgatca aatggcaatc taaggactga tcagtttcca
     5881 gctgattcac aaaatgacgg acaaccattt cattctccgg ttcataacag aagattctga
     5941 tattcacctc attgctgagt cctttgcctt ccaggaactc cggttcctga atcaatgccc
     6001 gaactttgtc caggcgttct tttatgtcac tcattggcta cctcctaaga aaagcaatta
     6061 aacgccggca gtatcgccat atcaccattg cttctgatgg cattctccaa aacaggatgc
     6121 agccatactg gattcaaatg atctgccccc cggttgtcga gatattctgt ttcaacaagc
     6181 actctggcaa ttatctgttt taactttgtt attgtagtat cgctccagga agccacggta
     6241 tcattctgct cctgcaagcg cataaaaaat gtattcaaat ctatcttacc aaaagacgtg
     6301 tcccttaatc tgtacttctc gccaatgacg gtcagcataa attcccagac cagtcggctc
     6361 tgcttcatca acgcatacaa acaaatctgt tttgccacat ccgtcggctg tgaagcaatc
     6421 gcagaaacca gagaatcatc ttccagtgcc tgaagccgtt tgatgcaggc tttcgccatg
     6481 cgtgtaatag atttttccgt tggatactga aaaagattct gctctacaat ttctttaaca
     6541 attgcatcat cggaatttcc ttctgccatt agctttgcgg tagaacgcat ttcgtagaaa
     6601 aggaacggct ctctggtcaa agacgcaata taaggataag aatgatccat gttcccgtac
     6661 ctccttactg ctcctctttt ttatcaggaa caaattccat gatatctccc acatcacaat
     6721 tcaaagcaga acatatcttt gacagaatct ctgtacttac attttcatct tttgataatc
     6781 gagacatcgt tgttgtacta actcctgtca ttgcacgcaa atctttcttc atcatttctt
     6841 tatcaattaa caatttccac aa
//
LOCUS       DBMDXG010000067         6854 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2843_length_6854_cov_1.800089, whole genome shotgun sequence.
ACCESSION   DBMDXG010000067 DBMDXG010000000
VERSION     DBMDXG010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2843_length_6854_cov_1.800089"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="ACZPD3_03215"
     CDS             complement(<1..187)
                     /locus_tag="ACZPD3_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1415048.1"
                     /translation="MTDNGKPKYFSLMEQLRSDIMSGAIRPGEKLPSENELSQKYSLS
                     RHTVRKALGILEQDGYVE"
     gene            complement(379..1266)
                     /gene="srtB"
                     /locus_tag="ACZPD3_03220"
     CDS             complement(379..1266)
                     /gene="srtB"
                     /locus_tag="ACZPD3_03220"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1415049.1"
                     /translation="MKKLRTFAVSMIIAAIGLLIYCAGFYIKSYEQNLAARQEYAELS
                     QMAGVKNREGAGLLADGKKTDEDSSTKNSEKKRRKKQRRSSESKNKNCTDEIRESFGI
                     SWENLRKINSQTVAWITVPGADISYPVVQAADDEYYLKHNFRGEEDLFGCIFLEHDIK
                     KNFTDSHSILYGHNIEGNMMFANLNRYEQPEFLKKCPEIEITTPKRKFLYKIFSVEQA
                     SSKSPAFEYGYKLSSPAYRRQLSILKNNSMYDTGVEPDERERMVTLITCNSRLDKEIR
                     MAVHGICHECYGIEKAEPK"
     gene            complement(1263..5972)
                     /locus_tag="ACZPD3_03225"
     CDS             complement(1263..5972)
                     /locus_tag="ACZPD3_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013508.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HIR1415050.1"
                     /translation="MKHNFKLKERLGALLLAMLFILQAILGLVPVCVTQAAPLTVETW
                     DSDKVVDYGYRFNMKFQPGITTYESFGCDNLDREAFSDNGKSERDTECVRVGADYKAG
                     SAGMRYNNVGKDGNGNIVDVRLILVGVENAEPRYDLRTAESIVQNKGGATFAWKDNEA
                     YPMVGFSKNSIGVFIYSVGYAKVKFQFLKHGTEETLPISGHGTIRDIDAGQGVRIPSD
                     SSLDNAYVLKNNDYLTVDGNSVSSPLGSVEPDDPRGWLNLFYDTDNFTVEFCHQFRLD
                     KWDKSREDAIAKAGSQERWAEITRNKYLDPSGNSYCPNFKGQKYCKAYAYFDFTSYCF
                     GDVEMKKAPEKRVGEANCTWEQAAAASKEKPFGIRQGQEFQYMIRAEVTPNRLKSFVV
                     QDILEDCLTIEDASKVSIVNDAGQTVTDWFDVAVEGQKVTCRAKAESLQDEAFTDNQT
                     YTFTLKVRQRPESEINISKYLAEDGYSILVPNHASMSYERTNGSGDTMDTETVWVKGV
                     IPPELEVKKNTSQYEWKTGDIIDYEVLVSQTKQDVKAVNVVITDELPSCLQLLEGQYA
                     VETSQGGENCTLTGQGENGWKAECPSLKYGETITIRFKCQASADSNGQEWENIVTATA
                     DNLINPDTGEQESRKDMAEVWPNSPQLEIDKTADKYEWQAGEQVAYRIVVNNVTAGTI
                     AKDVTITDIGLPQGLVLAGGTQSMEVLGVQQQVNYPVPDKKTGQAYEARPVDSQLNAD
                     ENGFSFYCSYVPYSQPVTIIFHCIAQEEANGHESVNAATVKAANTDERSDDAEVYVNS
                     GEFWIEKSADHYEWQVGEQVQYNVVVENKKAGTVARNVTVWDTGMPAGLALSSAEDVS
                     VSGIPQNITQLTAGTKDVLNQLNPEFYNETSEKPVNYEFLQEGSGWRLNISDLPANTP
                     VMISFLCTVTEAANGMESINVANVQAQNAPVSQDDAEVYVNTAVLSIEKSFQNPYLAA
                     GDGRAENEFRVGEQVNYQVTVNNLQKGSIARNLVISDLSLPEGLALDGAEDAVTVSGV
                     PAVIQNPVAGIDDAGNQLNPENYKETVEKPVSCQVTRQETGWIVTISDLPYQTPVTVN
                     FRCTAQESVNGMEIVNTAQAYADNAQKVKDSSKIWVNSPVLKVEKTTDKPFYKYGDII
                     TYRIVLTQEQTGCVARNVTLQDVIDTQGVRLLKDSVILMDEKGNVADADVQINDDNTF
                     LMSTGRTLVRDSRYSICDNDKGGLFEQVMYNPLDCQEQKSMIVEYQAAVIDAALAGQK
                     VHNTAVADSSEKIPATGEAETEVHSPILEIVKESDKKEYASGEKGYYKLTVRQLREDV
                     TDQNIVIEDKLETQGASIVKDSIFVKKNGIELKDAKIEADDTGFVIQTGASLSDMDKI
                     EVCYEMVFKTESTEPEKIVNTAKARGDISPEITAQQEAYVKAKAEPTATPTPSATPKP
                     TETPKPTETPKPTEVPKPTEQPKATPTPSVPGTCPKMTPVPTKAPLASYNGGNNGGTT
                     SGSGGSSYANSGGYSGGYGSYQGGSMAGSSKTGDVRPFKMMAVLGLIGMGLLTGGVVI
                     YRRTVSGKRNIKGMPRK"
     gene            complement(6176..6637)
                     /locus_tag="ACZPD3_03230"
     CDS             complement(6176..6637)
                     /locus_tag="ACZPD3_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HIR1415051.1"
                     /translation="MNRKEFLEILRSQLAGQMQEGKAAAHIRYYEDYIQSQVRGGRSE
                     QEVLQELGDPHLIAKTLIDTDDGSTQEDYGEYSSYGSSYGNETELPHQQEKRWKKVID
                     LSTWHGRAVVIAAAAVIIVLLILIIGVAIPFFIILAIILYFLSWLKKRNDQ"
BASE COUNT         1694 a         1716 c         1179 g         2265 t
ORIGIN      
        1 cctctacata tccgtcctgc tccaggattc ccagtgcttt acgaactgta tgtctactca
       61 gggaatattt ctgtgaaagc tcgttttctg aaggaagctt ctctcccggt cggatagctc
      121 ctgacataat atcacttctg agctgttcca tcagtgagaa atatttcggc tttccattat
      181 ctgtcatgtc ttcttccctc ctgtatttag gatacaactt gtacgcacgt acgaacaagt
      241 tgttttttat gcataaatta tacaaaatat cgagagattt ttcattagtt ttttccaaaa
      301 cacctcgagg gatagtgata tgtgaacagt aacagttcat cctttacgaa agttaccaat
      361 atccgattga tttcaacatc acttcggctc agctttttct ataccataac attcatggca
      421 aatcccatgt accgccatac gtatttcttt atccagacgt gaattacagg tgatcagagt
      481 caccattctc tccctttcat caggctctac tcctgtgtca tacatggaat tatttttcaa
      541 tatagataat tgtctcctgt atgcaggact ggataacttg tatccatatt caaatgccgg
      601 gcttttggaa gatgcctgtt ccacagaaaa aattttgtac agaaactttc tttttggtgt
      661 tgtgatctca atttcaggac attttttcag aaattcaggc tgttcataac ggttcagatt
      721 tgcaaacatc atattacctt ctatattatg cccgtaaaga atactgtggg aatctgtaaa
      781 attctttttt atgtcatgtt ccagaaaaat acatccaaac agatcttcct ctcctctgaa
      841 attatgtttc aggtaatatt catcatccgc cgcctgaact acaggataag aaatatcggc
      901 tcccgggaca gtaatccagg caactgtctg agagttgatc tttcttaaat tttcccagga
      961 aatcccgaag gattccctga tttcatctgt acagtttttg ttttttgatt cagaagatct
     1021 tctctgtttt ttcctgcgtt ttttttcact gttttttgta ctgctgtctt catcagtttt
     1081 tttgccatct gccaataatc cggcaccttc tctgttcttc acccctgcca tttgggaaag
     1141 ctctgcatat tcctgccttg ctgcaagatt ctgttcataa ctttttatat agaaaccggc
     1201 acaatatata agaagcccaa ttgctgctat gatcatactt actgcaaatg ttctgagttt
     1261 tttcatttcc gcggcatacc ctttatattt cttttcccgg aaacagttct tctgtagatc
     1321 accacacctc ctgtcagcag tcccattccg ataaggccaa gaacagccat cattttaaac
     1381 ggtctgacat ctcctgtttt agaacttcct gccatggaac caccctgata gcttccgtat
     1441 ccaccggaat atccacccga atttgcgtaa ctgctgcctc cgcttccgga agtcgtgcct
     1501 ccattatttc caccattgta agatgctaac ggtgctttcg tcggtacagg agtcattttc
     1561 gggcaggttc ccggtacaga tggtgttggt gtggcttttg gctgctccgt cggtttcggt
     1621 acttctgtag gttttggtgt ctctgtcggc tttggtgttt ctgttggttt cggtgttgca
     1681 gacggtgttg gtgtggcagt cggttctgct tttgctttca catatgcctc ctgctgtgca
     1741 gtaatttccg gtgaaatatc acctcttgct ttcgctgtgt ttacaatttt ttcaggctct
     1801 gtgctttctg ttttaaatac catttcatag cagacttcaa ttttatccat atctgacaac
     1861 gatgctcctg tctgaattac aaatcctgta tcatctgcct ctattttggc atctttcagt
     1921 tctataccgt ttttcttcac aaaaatgctg tcttttacaa tggaagcccc ttgggtttcc
     1981 agtttatctt cgataacaat attctgatct gtgacatcct cacgcagttg tcttactgtc
     2041 agtttatagt atcctttttc accggacgca tactctttct tatctgattc cttcacaatt
     2101 tccagaatcg gactgtgtac ctctgtctct gcttccccgg ttgcaggtat cttttcactg
     2161 ctgtctgcaa ctgcagtatt gtgcactttc tgtcctgcca gggctgcatc aatcactgct
     2221 gcctgatatt ccacgatcat acttttctgt tcctgacagt ccagaggatt atacattacc
     2281 tgctcaaaaa gaccgccttt atcattatcg cagatactat atctgctatc cctgaccagt
     2341 gtcctgcctg ttgacatcag gaaagtatta tcatcattga tctgtacatc agcatctgcc
     2401 acatttcctt tctcatccat cagaatcacc gaatccttca gaagacgtac tccctgagta
     2461 tcgatcacat cctgcagagt aacattacgc gccacacatc cggtctgctc ctgtgtcagg
     2521 acaatgcggt atgtaataat atcaccatat ttgtaaaatg gtttgtccgt cgtcttctct
     2581 actttaagaa caggcgagtt cacccagatt tttgaggagt cctttacttt ctgtgcatta
     2641 tctgcatacg cctgtgctgt atttacaatc tccatcccat ttacgctttc ctgcgcagta
     2701 cagcggaaat taacagtcac aggtgtctga tacggaagat ctgaaatcgt cacgatccat
     2761 ccggtttcct gacgtgtaac ctgacagctg actggttttt ccactgtttc tttataattt
     2821 tccggattca gctgatttcc tgcatcatca attcctgcca ccggattctg aataacagcc
     2881 ggaacaccgc ttacagtaac agcatcttca gctccgtcca gagccagccc ttcaggaaga
     2941 gaaagatcac tgatcacaag atttcttgcg atagatccct tctgaagatt attaaccgta
     3001 acctgataat tcacctgttc tcctacccga aattcatttt ccgcacgtcc gtccccggct
     3061 gccagatatg gattctgaaa acttttctca atagaaagaa ctgccgtatt tacatacact
     3121 tctgcatcat cctgcgaaac aggggcattc tgcgcctgca cattggcaac attaatgctt
     3181 tccattccat ttgccgcttc cgtaactgtg caaaggaaag aaatcataac cggagtattt
     3241 gccggaagat ctgaaatgtt cagtctccat ccgcttccct cctgaagaaa ttcataattc
     3301 accggtttct ctgatgtctc attataaaat tccggattga gctggttcaa aacatctttg
     3361 gtccctgctg tcagctgcgt aatattctgt gggattccgc ttaccgatac atcctctgcc
     3421 gatgaaagcg caagtcctgc cggcataccg gtatcccaga cagttacgtt ccgggccact
     3481 gtacctgctt ttttattctc caccaccaca ttgtactgta cctgttctcc gacctgccat
     3541 tcataatgat ctgcagattt ctcaatccag aattctcctg aatttacata tacttctgca
     3601 tcatcagatc tctcatctgt attagcagct tttacagttg ctgcatttac gctttcgtgt
     3661 ccatttgcct cttcctgtgc gatacaatgg aaaataatcg ttacaggctg actgtatggt
     3721 acataagaac aataaaagga aaatccgttc tcatctgcat tcaactgaga atccacaggt
     3781 ctcgcttcat atgcctgtcc tgttttctta tccggtacgg gatagtttac ctgttgctga
     3841 actcccagta cttccatgct ctgtgtacca cctgccagaa ccagtccctg cggcagacca
     3901 atatcagtaa tagttacatc ttttgcaatt gttcctgccg ttacattatt cactacgatc
     3961 cggtaagcta cctgctctcc tgcctgccat tcatatttgt ctgctgtttt atcaatttca
     4021 agctgtggac tgttcggcca tacttctgcc atatcctttc tggattcctg ttctcctgtg
     4081 tcaggattga tcagattatc cgctgttgca gtcacaatat tttcccattc ctgtccattg
     4141 gaatccgcag atgcctgaca tttaaaacgg attgtgatcg tctccccata cttcagggac
     4201 ggacactctg ctttccatcc attttctccc tgtccggtaa gcgtacagtt ctcgcctccc
     4261 tgacttgtct ctacagcata ctgcccttcc agaagctgca gacatgatgg aagctcgtct
     4321 gtaataacaa catttactgc ttttacatcc tgttttgtct gagaaaccag aacctcataa
     4381 tctatgatat ctcccgtttt ccattcatac tgagaagtat ttttctttac ttccagttcc
     4441 ggtggaatca caccctttac ccacactgtt tccgtatcca ttgtatctcc gcttccgttt
     4501 gtacgttcat aactcatgga tgcatggttt ggtacaagaa tactgtatcc atcctctgca
     4561 aggtattttg aaatgttaat ttctgattcc ggtctctggc gtacttttaa agtaaatgtg
     4621 taagtctgat tatctgtaaa tgcttcatcc tgaaggctct ctgctttggc cctgcaggtc
     4681 actttctgtc cttcaacagc aacatcaaac cagtctgtca ctgtctgccc tgcatcattc
     4741 acaatactga cttttgatgc atcctctata gtaagacaat cctcaagtat atcctgtacc
     4801 acaaaggatt tcagtctatt gggtgtcacc tctgcccgga tcatatactg aaattcctgt
     4861 ccctgacgaa tgccaaaagg tttttctttt gatgcggcag ctgcctgttc ccatgtacag
     4921 tttgcctctc ccacacgttt ctcaggcgct tttttcatct caacatcacc gaaacaatag
     4981 gatgtaaaat caaaatatgc atatgctttg cagtatttct gtcctttaaa gttcggacaa
     5041 taactgttgc ctgatggatc cagatattta tttcttgtaa tctctgccca tctttcctga
     5101 gaaccggctt ttgcaatggc atcttcacga cttttatccc atttatccag tctgaactga
     5161 tgacagaatt ctaccgtaaa attatctgta tcataaaaaa gattcagcca tcctctcgga
     5221 tcatccggct caacggaacc aaggggtgag cttacagaat ttccatctac cgtcagataa
     5281 tcattgtttt tcagaacata tgcattatcc agactgctgt ctgacggaat acgaactccc
     5341 tgtcccgcat caatatcacg tatggttcca tggccactga tcggcagtgt ttcttctgtt
     5401 ccatgcttca gaaactgaaa cttaaccttt gcgtatccta cagaataaat aaatacaccg
     5461 atactgtttt tggaaaagcc aaccattgga taagcttcat tatctttcca tgcaaacgta
     5521 gctccgcctt tattctgaac aatcgattct gcagtgcgca gatcataacg gggttctgca
     5581 ttctctactc ccacaagaat gagtctgaca tcaacaatat ttccatttcc atcttttcct
     5641 acattgttat aacgcatccc tgcgcttccg gctttatagt ccgctcctac acgaacgcac
     5701 tctgtatcac gttcgctttt tccattgtct gaaaaagctt ccctgtccag attatcacac
     5761 ccaaaagatt catatgtggt aattccgggc tgaaacttca tgttaaagcg ataaccataa
     5821 tctaccacct tgtcactgtc ccatgtttct acagttaacg gtgccgcctg agttacgcag
     5881 acaggcacaa gccccagaat tgcctgcaga ataaaaagca ttgcaagcag caaagctcca
     5941 aggcgttctt tcagtttgaa attgtgtttc attttccgtc tcctttccga acaaaaaata
     6001 taaaaatgaa tgtgggattt ttccctgcac ctggcaggat taaagaatag aaataagttg
     6061 agattgtatg attataaata ttcaagggga attaatatcg tgaaacaaga acgtttacac
     6121 ttaagatttt taagattctg cgcagggttt cttttctgaa accctgcaca gattttcatt
     6181 gatcatttct tttcttcagc catgaaagaa aataaagaat aattgcaaga ataataaaaa
     6241 acggaatcgc aactccgatt atcagtatca gtaaaacaat tataacagcc gccgctgcaa
     6301 taacaacagc cctgccatgc caggtagaaa gatcgattac ttttttccat cttttctcct
     6361 gctgatgagg aagctctgtc tcattgccat aactgcttcc gtaagaagaa tactctccgt
     6421 aatcttcctg tgtacttcca tcatctgtat cgatcagagt ttttgctatc agatgcgggt
     6481 ctcccagttc ctgcagtact tcctgctctg aacgtccgcc acgtacctga gactgtatat
     6541 aatcttcata atacctgata tgtgctgctg ctttgccctc ctgcatctga ccggcaagct
     6601 ggcttctcag gatttccaaa aattcttttc tgttcatctt ctacactcca ttttgccaga
     6661 aatacatgtc aaaactctac tgctcgctcc gttatcagta acgttcgtac tcttatttta
     6721 ccatatatac cgcaggtagc aactaatata atcataaact ttttatgaaa ttcttataaa
     6781 ctcaattact gttcactccg ttctcagtaa ctgagccaaa attcattcca gattgcctgc
     6841 ggcaatggaa tttt
//
LOCUS       DBMDXG010000068         6793 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2878_length_6793_cov_1.819934, whole genome shotgun sequence.
ACCESSION   DBMDXG010000068 DBMDXG010000000
VERSION     DBMDXG010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_6793_cov_1.819934"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="ACZPD3_03235"
     CDS             complement(<1..1084)
                     /locus_tag="ACZPD3_03235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HIR1415052.1"
                     /translation="MVINVITRHAPPNYGSLLQAIATQRVIMNLGHECRIINYIPKCE
                     TGVRMAITQLEQKTKWRRNPIKKAIYLMVAEPKTLLMDRKFLAMRKKYLLMGPYCATT
                     GELKKLYAEKKDEVFLTGSDQVWGPISTGHYDPTYFLDFVPKSSRKLAFAASFGKAIF
                     DEQTLKEYAVLLKKYDSLAVRENVAVELLKKMDISAKQVLDPTLLMDADAWSEYVKPM
                     KKPEKYVLVYQIHANSDLDHYAVKFAEKAELPLLRVSPLLHQARRSGKFVYCPDISGF
                     LDLVKNAAYMVTDSFHGTAFAINFNTQFVEVLPNTGTSSRNQSILEFTGLTNRIVRDL
                     NDFSYIDQEIDFKEANEKIGISRIESI"
     gene            complement(1089..2300)
                     /locus_tag="ACZPD3_03240"
     CDS             complement(1089..2300)
                     /locus_tag="ACZPD3_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016328.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HIR1415053.1"
                     /translation="MGVVLFENKSDCCACGACLNVCSKSAISMKPDAEGFLYPEIDPN
                     KCVECGVCKRVCKYKTSDLNVVESTYAVTNRDEKQIMKAASGGLFSAAASAILSDGGC
                     VFGATLTFDSGHPDTHHIMVENKEDLWKLQGSKYVQSRIGRTYQQAKECLQHGRTVLF
                     SGTPCQIAGLKSYLGKVYENLYTMDLICHGVPSAEFFDGYIQELNKKYRGKVTEYKFR
                     DKTKGWGMNTAFEIVRGDKKKTIFKTAKVQSYLSLFYDCKIFRENCYSCPYAGSSRVG
                     DVTIGDYWGIENEHPELDHDPDFDKEKGISCLLINSPKGQELFELIENQIHFVPSQFA
                     KVAKTNGQLKAPSVMPMERNNILDISRRNGYEGVERYFSRKYCHQILLYTGYDLIPQK
                     VRKRLKKILKR"
     gene            complement(2335..>2985)
                     /locus_tag="ACZPD3_03245"
     CDS             complement(2335..>2985)
                     /locus_tag="ACZPD3_03245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1415054.1"
                     /translation="ACGSEAGRFLYRNDDFKVIPNAVDAEQFYFNADVRADKRKELGL
                     TDQFVIGHVGRLSYQKNHRFLLKIFAEIVKKEQNAILLLAGTGEKEQELRALVEQLAL
                     NNQVVFLGNRSDVAELYQAMDVFVLPSHFEGIPVVGVEAQFADLPCVFSDKVPSEVAF
                     SEKVCFLPLSDSPEAWANRIIACRGQERSSDRNEIVNSHYDIKTAYKILQDYYLAF"
     assembly_gap    2986..2995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2996..3373)
                     /locus_tag="ACZPD3_03250"
     CDS             complement(<2996..3373)
                     /locus_tag="ACZPD3_03250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1415055.1"
                     /translation="MNYYRNIDRERVQFDFLTHRPEKSDYDDEIVSLGGKVYYAPRLY
                     PQNYPAYFKYMKQFWAEHPEYKIVHSHIDSMSYLPLLTAKKAGVPVRIAHSHNTSIDK
                     DFKYILKQLFRYSINSVANYHLAC"
     gene            complement(3471..4721)
                     /locus_tag="ACZPD3_03255"
     CDS             complement(3471..4721)
                     /locus_tag="ACZPD3_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR1415056.1"
                     /translation="MREFKDLKIAVAGTGYVGLSIATLLSQHHKVTAVDIIPEKVELI
                     NNKKSPIQDEYIEKYLAEKELDLTATLDAREAYSDADFVVIAAPTNYDSKKNFFDTSA
                     VEAVIKLVIEYNPEAIMVIKSTIPVGYTASVREKFHCDNIIFSPEFLRESKALYDNLY
                     PSRIIVGTDVDNARLVKVAHTFAELLQEGAIKENIDTLFMGFTEAEAVKLFANTYLAL
                     RVSYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLAN
                     YADVPENLIEAIVESNRTRKDFIADRVLEIAGAYEANDCWDESKEKEVVVGVYRLTMK
                     SNSDNFRQSSIQGVMKRIKAKGATVIIYEPTLKDGETFFGSKVVNDLAKFKEMSQAII
                     ANRYDSCLDDVKDKVYTRDIFQRD"
     gene            complement(4743..5792)
                     /locus_tag="ACZPD3_03260"
     CDS             complement(4743..5792)
                     /locus_tag="ACZPD3_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1415057.1"
                     /translation="MSIVTLDKKTILVTGAAGFIGSNLVKRIYQEAPSATVIGIDNMN
                     AYYDVALKEFRLNELAKYPTFTFVKGNIADKALITELFEKYKPSVVVNLAAQAGVRYS
                     ITNPDAYVESNLVGFFNILEACRHCESLEHLVYASSSSVYGSNKKVPYSTDDKVDNPV
                     SLYAATKKSNELMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYFGFTNKLVKGETIK
                     IFNYGNCKRDFTYVDDIVEGVVRVMKKAPDKKNGEDGLPIPPYAVYNIGNQNPENLLD
                     FVQILSEELVRAKVLPEDYDFEAHKELVPMQPGDVPVTYADTSALERDFGYKPSTSLR
                     TGLRKFAEWYAEFYK"
     gene            complement(6095..>6793)
                     /locus_tag="ACZPD3_03265"
     CDS             complement(6095..>6793)
                     /locus_tag="ACZPD3_03265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR1415058.1"
                     /translation="EKNAVKYADEIIVLSKGVQDYFRDTYGRETHFIPNGVNRPETRE
                     AGLITEKFGLTKDSYILFLGRLVPEKGIRYLVEAFKDVKTDKKLVIAGGSSDTDSFMM
                     ELKELAKDDNRIIFTGFVQGQMLDELYSNAYIYTLPSDLEGMPLSLLEAMSYGNCCLV
                     SDIPECAEVVEDKALIFKKSDVDELREKLQDACDYPEKVKEMKAQAADFICKKYNWDA
                     VINETMKLYRRNNK"
BASE COUNT         1854 a         1675 c         1228 g         2026 t
ORIGIN      
        1 gaatggattc tattctactt ataccaatct tttcattcgc ttccttaaaa tcaatctcct
       61 gatcgatata tgaaaaatca ttcaaatctc gaacgattct atttgtcagt ccagtaaatt
      121 ctaaaatact ctgatttctg ctgctggttc ctgtattcgg aagcacttcg acaaactgtg
      181 tattgaaatt gatagcaaaa gctgtaccat gaaatgaatc tgttaccata taggcagcat
      241 tttttacgag atccaaaaat ccgctaatat cagggcagta cacaaacttt ccgctacgtc
      301 ttgcttggtg aagtaacggt gaaacgcgga gcaatggtaa ctctgctttt tcagcaaact
      361 tcactgcgta gtgatccaaa tccgaatttg catggatctg ataaacaagt acatattttt
      421 caggtttctt cattggtttg acatattctg accaagcatc agcgtccatt aataaagtag
      481 gatcaagtac ctgctttgcc gaaatgtcca ttttttttag gagttcaacc gcaacgttct
      541 ctctaacggc caaactgtca tattttttta aaagcactgc atattctttg agtgtttgtt
      601 catcaaatat tgcttttcca aaactggccg caaaagcaag cttgcgtgaa cttttcggga
      661 caaaatcaag gaaataagtt ggatcgtaat gtccggtaga gataggtccc cacacctgat
      721 cgcttccggt aagaaaaact tcgtcctttt tctcagcata caatttctta agctcccctg
      781 ttgtggcaca atagggtccc atcagcaggt atttttttct catcgccaag aattttctat
      841 ccatcaacaa agttttaggc tccgccacca ttagatatat cgctttttta atcggattgc
      901 gtctccactt tgttttttgt tcaagctgcg taatcgccat cctaacgcca gtttcgcatt
      961 tcggaatata atttattatt cgacactcat gccccagatt cataatcact ctttgtgttg
     1021 caattgcctg taacaaagaa ccataattcg gcggagcatg tcttgtaatc acatttataa
     1081 ccatcaaatt acctcttcaa aattttcttc aacctcttgc gcactttttg cggaatcaaa
     1141 tcatatccag tatagagtaa aatctgatga caatatttcc tgctgaaata gcgctctaca
     1201 ccttcatatc cgtttcttct actaatgtcc aagatattat tacgttccat tggcatgact
     1261 gaaggtgcct ttaactgccc attggtcttc gcaacctttg caaactggga cggaacaaaa
     1321 tgaatctgat tttctataag ttcgaataat tcctgtccct taggggaatt gattagaagg
     1381 caagaaatgc ctttttcttt atcaaagtca ggatcatgat ccaactcagg atgttcgttt
     1441 tcaatacccc aatagtcccc aattgtcacg tctccaactc tgcttgagcc ggcatacggg
     1501 cagctgtagc agttttctcg aaatatttta caatcataga aaagcgaaag atatgactga
     1561 acttttgcag ttttaaaaat cgtcttcttc ttatccccgc gaacaatctc aaaggcagta
     1621 ttcatccccc aaccttttgt tttatcgcgg aacttatact ctgttacttt gccacgatat
     1681 ttcttattca gctcttgaat ataaccatca aagaattcgg cagagggaac gccatggcaa
     1741 atgaggtcca ttgtataaag gttctcatac actttcccca aatagctttt taagccagct
     1801 atctggcacg gtgtaccaga aaagagaacc gttcgcccat gctgcaggca ttcctttgcc
     1861 tgctgatatg tcctgccaat ccgactctgc acatacttcg atccttgcag cttccaaaga
     1921 tcctctttat tctctaccat gatgtggtgt gtatcggggt gaccgctatc aaatgttaat
     1981 gtcgcaccaa acacacaccc accatccgac aagatagctg aagccgctgc tgagaacaag
     2041 ccaccggaag cagccttcat aatttgcttc tcatctctat ttgtcacagc gtatgtactt
     2101 tcaacaacat tcaagtctga tgttttatac ttgcaaactc tcttgcagac gccacattcc
     2161 acgcacttgt tgggatcgat ttcaggatac aaaaatccct ccgcgtctgg tttcattgag
     2221 atcgcacttt tggaacagac attcaggcaa gctccgcatg cgcagcaatc acttttattc
     2281 tcaaataata caactcccat ttattccctc cacgaaaaat attgcaaata ccaatcaaaa
     2341 ggcaagatag taatcctgca aaattttgta cgcagttttg atatcataat ggctatttac
     2401 aatttcattc cggtcagaac ttctttcctg ccctctacac gcaataattc gattagccca
     2461 cgcttccgga gaatcactca atggaagaaa acatactttc tcactgaatg cgacttcgct
     2521 ggggacctta tcagaaaaaa cacaaggcaa gtccgcgaac tgagcttcaa cacccaccac
     2581 tggaattcct tcaaagtggg atggtaacac aaaaacatcc atggcctgat aaagctctgc
     2641 gacatcgctc ctattgccaa gaaacaccac ctggttgttc aatgcaagct gctcgaccaa
     2701 cgctctaagt tcctgctcct tttcgccggt gcctgccagc agcagaatcg cgttctgttc
     2761 ttttttgaca atttcagcaa aaattttcag caggaaccta tggtttttct gataagacaa
     2821 acgcccaaca tgtccgataa caaactgatc tgtgaggccc aattcttttc tcttatcagc
     2881 cctgacatct gcattgaaat agaactgctc agcatcaacc gcgttcggaa taaccttgaa
     2941 atcatcattt ctgtacagaa accgtcccgc ttccgatcca cacgcnnnnn nnnnnacacg
     3001 ctaagtgata gttagcaaca gagttaatac tatatcggaa cagttgcttc agaatatact
     3061 taaaatcctt gtcaatagag gtgttatggc tgtgagcaat tctcacaggt acacctgcct
     3121 tcttcgctgt cagaagaggg agatagctca tggagtcaat atgtgaatga acgattttgt
     3181 actccggatg ctccgcccaa aattgcttca tatatttgaa atatgccggg taattctgtg
     3241 gatacaatct cggagcatag taaaccttac ctccaaggct aacaatttca tcatcgtaat
     3301 cgcttttctc cggtcggtgc gtcagaaagt cgaactgaac tctctcacga tcaatattcc
     3361 gataataatt catcagcatc gtttccaacc cagcgcgatg catgtcattt acaacttgta
     3421 aaattcttat cactgtccgt tctcctttct gcgccgttct atcgtctcca ttaatccctc
     3481 tggaaaatat ctcttgtata aaccttgtcc ttcacatcat ccaagcaact atcatatcta
     3541 tttgcaataa tcgcctggct catttctttg aacttcgcca gatcatttac aaccttactt
     3601 ccgaagaaag tctcgccatc tttcagagtc ggttcgtaaa taattacggt tgctcccttg
     3661 gccttaatac gcttcatgac accctgaatg gagctctggc ggaagttatc gctgttactc
     3721 ttcatggtca ggcggtatac acccacaaca acttcttttt ctttgctttc atcccagcag
     3781 tcatttgctt cataagcacc agcgatctcc agcacacgat cagcaatgaa atcttttctt
     3841 gtcctgttac tctcaacaat ggcctcaatc aggttttccg gcacatcagc ataattggcc
     3901 agcagctgct tggtgtcctt cggcagacag tatccaccgt aaccaaagct gggattgtta
     3961 taatgcgtgc cgatacgagg atcaaggcag acaccattga tgatctgctg agtgttcagc
     4021 cccttcatct cggcataggt atccagttca ttgaagtagc tgacacgcag tgccagatag
     4081 gtgttggcga acagcttaac tgcctctgcc tcggtaaagc ccatgaacag agtgtcgata
     4141 ttttccttga ttgcaccttc ctgcaggagt tctgcaaaag tgtgtgctac cttcaccaga
     4201 cgggcattat ccacatcggt accgacaatg atacgggaag gataaaggtt gtcgtacaga
     4261 gccttactct cacgcagaaa ttccgggcta aaaatgatgt tgtcacagtg gaacttctca
     4321 cgaacgcttg ctgtataacc aaccggaata gtgctcttga taaccatgat agcttccggg
     4381 ttgtactcga tgaccagttt aatgacggct tccaccgcac tcgtgtcaaa aaagttcttc
     4441 ttgctatcgt aatttgtagg agctgcgatc actacaaaat cagcatcact gtatgcttcc
     4501 ctagcatcca gagtcgcagt cagatccagc tctttctctg ccaaatactt ttcaatatat
     4561 tcatcctgaa tcggagattt cttattattg ataagttcaa ctttctcagg aatgatatcc
     4621 acagcagtca ccttgtggtg ctgagataac agcgtagcaa tagaaagacc aacatatccc
     4681 gttccggcaa cagcaatctt taaatcttta aactctctca tttctctatc cctctaattc
     4741 ttttatttat aaaactcagc gtaccactca gcgaactttc ttaatccagt ccgcagactc
     4801 gtgctcggtt tgtacccaaa gtcgcgctcc agtgcgctag tatctgcata ggtcaccggt
     4861 acatcaccag gctgcatcgg aaccagctcc ttgtgagcct cgaagtcgta atcttccggc
     4921 agaaccttcg ctcttactaa ctcctcactc agaatctgca cgaagtccag cagattctcc
     4981 gggttctgat taccgatgtt gtaaactgca tacggcggaa tcggcagacc atcttcaccg
     5041 ttcttcttgt ccggtgcctt cttcatcaca cgaacaacgc cctcaacgat gtcatccaca
     5101 taagtaaaat cacgcttgca gttaccgtag ttgaagatct tgatggtttc gcccttcacc
     5161 agcttgttgg tgaaaccgaa gtaagccatg tccgggcgac ctgcagggcc atacaccgta
     5221 aagaatctca ggccagtgga aggaatgttg tagagcttgg agtatgcgtg tgccatcaat
     5281 tcattagatt tcttggttgc tgcataaagg gaaaccggat tgtcaacctt gtcatccgtg
     5341 ctgtatggaa cctttttatt agaaccatag acggaggagg aagaagcata aaccaaatgc
     5401 tccagactct cacaatgacg gcaggcttcg aggatattaa agaagccgac caagttagat
     5461 tccacataag catctgggtt ggtgatggag tagcgcacac cagcctgtgc tgcaaggttg
     5521 acgaccacag acggcttgta cttctcgaac agctcggtga tcagtgcctt atcagcgatg
     5581 ttgcctttta caaaagtgaa tgtgggatac ttggccagct cgttcaggcg gaattctttc
     5641 agtgccacat catagtaggc attcatattg tcgatgccga tgaccgtagc agaaggagcc
     5701 tcctgataga ttcgtttcac aaggttggag cctatgaaac cggctgcacc tgtgactaaa
     5761 atcgtttttt tatctaaagt tactatactc attgcattcg tctccttatt tttgaagcgt
     5821 atatatcaag tgtcactacc tctgttcaga tgaagcactt ccaatgtatt gttcataaac
     5881 cagagtgctc catacaaacg caggtagtga tactgaaagt tcttatgata aaatcatctg
     5941 ctgttgctaa acctaggtag caagataaaa accgtactcg cagcttcacc catctctcga
     6001 aaagcgccgt aagtgcgttg ttttccaagc gttttcacta tgtacatctc actacctgcg
     6061 ttcgattaga ggagattatt tattatcaat actctcattt attatttctc ctgtacagtt
     6121 tcatcgtttc atttataaca gcatcccagt tgtatttttt gcagataaag tcagctgcct
     6181 gcgctttcat ctccttaact ttttctggat aatcacaggc atcttgtaat ttttctctaa
     6241 gttcatccac atccgatttt ttgaaaatca atgccttatc ttccacaacc tcagcgcact
     6301 ctggaatatc agaaaccaaa cagcaattgc cataactcat agcttccagc aggctcaacg
     6361 gcattccctc cagatcagaa ggtaatgtgt agatatacgc attgctatat aattcatcaa
     6421 gcatctgccc ctgaacaaat ccggtaaaaa tgattctatt atcatccttt gccagctctt
     6481 tcagttccat cataaaagaa tccgtatcgc tagagccacc agcaataacc aattttttat
     6541 ctgttttgac atccttgaac gcttcaacca aataccggat acctttttcc ggtaccagtc
     6601 gaccaagaaa taatatgtag gaatctttcg tcagtccaaa tttttctgtt attaaaccag
     6661 cttctcgggt ttctggtctg ttcacaccat tcggaatgaa atgtgtctcc ctgccatagg
     6721 tatctctgaa ataatcctga acacctttac tcagaacgat gatttcatct gcatacttta
     6781 cagcattctt ttc
//
LOCUS       DBMDXG010000069         6717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2907_length_6717_cov_1.930623, whole genome shotgun sequence.
ACCESSION   DBMDXG010000069 DBMDXG010000000
VERSION     DBMDXG010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2907_length_6717_cov_1.930623"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            252..1730
                     /locus_tag="ACZPD3_03270"
     CDS             252..1730
                     /locus_tag="ACZPD3_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1415059.1"
                     /translation="MNKKVIAAGMAMVLASMGLTACGDSGSKEAKDSSDETYTVTMAY
                     IGDKEEDTDRIEKKINEIMKKDINMELDIEPISWGAYAETMKLILSGGEKMDIVPILV
                     EQVNSMVNAKQVIDMSEYIDKYGNNIKELLGDTAKAANIGNYVYGVTTGREWFCQSSV
                     IMRKDILDECGIDVSSITDYKDLTDVYATVKEKYPDMVMMASNNSATPDTKYEMNDTL
                     TDGFGVLMDHGQDTTVVDYYETDEYKEFVETMYDWQQKGYLSKDAATTTESAENQVKA
                     GAAFSYLAPNKPGYDTRAALLCGTEMEIAPISEPWAGTAQISYLTYGISSSSADKDKT
                     MQCLDYLYGNADILNLLNWGEEGVDYEVVDAENNIINYPDGKDDSNTYHLAEGWQLFD
                     QFKMHIWEGDSPDIWDETKALNESAIKSKAFGFTYDSTSVANELAALSNVKAKYAAAL
                     GSGTVDPEETLPKFIEELKKAGIEKVISTKQEQLDKWLEENK"
     gene            1794..2699
                     /locus_tag="ACZPD3_03275"
     CDS             1794..2699
                     /locus_tag="ACZPD3_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415060.1"
                     /translation="MKKAKFKRWVPLYLMMAPGLIYLFINNYMPMAGLVVAFKNYNVV
                     DGIFGSPWAGLSNFTYLFNDAWTITRNTLLYNIVFIIINLILGIAFAIFICDIRSKAC
                     KTIYQSAILLPFLMSIVIVSYITFAFFSGDNGMLNKTILPFFGKEAINWYSESKYWPV
                     ILVIVNTWKGVGYGCLIYISSISGIDPSFYEAAELDGASKWKQIRYITLPSIMPSVIT
                     LTLLNIGRIFYSDFGLFYQVTQNSGQLYDTTNVIDTYVYRALLQSGNIGMASAAGFYQ
                     SIVGFACVLLANVVVRKLSPENAMF"
     gene            2710..3600
                     /locus_tag="ACZPD3_03280"
     CDS             2710..3600
                     /locus_tag="ACZPD3_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415061.1"
                     /translation="MVRSKSAKRFERLAHTVMILVTICIVLPFILLFMSSITSESALV
                     RDGYSFFPKEFSIHAYKFIWDNAANVFRAYGITILVTVIGTSINVAMSALLAYPLSLK
                     NLPGKRILNFYIFFTMLFNGGLVPTYLMYTGIFHINNTLLAYIVPGLLMSAMNVMLIR
                     TFFATSIPDALFEAAQIDGASQFQIFFKIVLPLGKPILIAMGLLSGLGYWNDWTNGLY
                     YIRDTKLYGIQNLLNKMISDLTALTQNASGASTVAIADIPSASVRMAIAFVAMLPILC
                     LYPFLQRYFTKGIALGAVKG"
     gene            3763..>4945
                     /locus_tag="ACZPD3_03285"
     CDS             3763..>4945
                     /locus_tag="ACZPD3_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1415062.1"
                     /translation="MNDYKLDLEKYATLARQAAAEGCVLLENEKQALPLREGESVAVF
                     GRMAFHYYKSGLGSGGLVNTRYVVGILDALKECKEIQLDEKLLGIYANWIKENPYDEG
                     QGWGRVPWSQKEMEVTEEMLDCARSNDVSLVIIGRTAGEDQDNNTNLGSYCLTETEED
                     LICRVCEVSKCTVVVLNVGNIIDMSWVEKYHPQAVLYAWQGGQEGGNGVADVLTGKVC
                     ACGKLTDTIAERIEYYPSTENFGDPYKNYYKEDIYVGYRYFETFAKDKVLYPFGYGLS
                     YTNFETKAEIFKNTEDELTVAATVTNIGDVRGKEVVQVYVKAPQGKLGNPARKLIGFA
                     KTRELAPGEKEELVIIIPKYDMASYDDSGVTGHKSCYVLEEGTYEIFAGSDVRSAKSA
                     GI"
     assembly_gap    4946..4955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4956..6521
                     /locus_tag="ACZPD3_03290"
     CDS             <4956..6521
                     /locus_tag="ACZPD3_03290"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR1415063.1"
                     /translation="VIEQLQEAYAPIEKFRRMKAVLRADGTYQAVTEEVPVRTADPHK
                     RREERMPKTLEYTGDKGYKLADVLDKKVSMDEFVAQISEADLIAMFRGEGMCSPKVTA
                     GTAAAFGGVTESLKALGIPVGCCADGPSGIRMDCGTKAFSLPNGTLLGCTFNTELVGE
                     LYEMTGRELRLNKIDSLLGPGMNIHRNPLNGRNFEYISEDPLLTGRICAAQVKAMAKS
                     EIGSTIKHFCGNNQEVGRSTSDSVMSERCLREIYLKGFEIAVKEGGARSVMTTYGSVN
                     GLWTAGSYDLCTTILRKEWGFQGIVMTDWWAKSNYEGHQAEVTAKAPMVAAQNDIYMV
                     VSDAKSNPENDDVEEMLHAGKITVGELQRNAANILGFLLKSPSVLLLTDRICKEELEA
                     MNTKEEDDVDAGSLVSIESDSVTQKIVIDGALLHPAKGKADVIAVTNEFMGDFTMKFT
                     LKSDLGELAQLPVSVFLDNIHKMTVSVQGTNGKWVEESRILNMEFGHNHYIKFYYGAD
                     NFEIKEIVLIPNR"
BASE COUNT         2075 a         1023 c         1688 g         1921 t
ORIGIN      
        1 atacctcctg aaatattata aaatcattat gatgaaaaca attataacaa aaatccgata
       61 aaaagataac aatattccga ccttttgata caacaaaaga gacaagctat gaaaaaagaa
      121 atcggatttc ggaaagtgtg aggtcgttat tttactatta aaatactgga aaatctgtat
      181 aatgagaaac atcagacaga gagatgtata catcaaaaaa aattaaaaag gaaaagaatt
      241 gaggtaaaag gatgaataag aaagtgattg cagcgggaat ggctatggta ctggcttcta
      301 tggggctgac tgcctgtggt gattccggtt caaaagaggc taaagattct tcagatgaga
      361 cttatacagt tacaatggct tatattggag ataaggagga agatacagat cgcatcgaga
      421 agaaaatcaa tgagattatg aagaaagata tcaatatgga actcgatatc gaaccgatca
      481 gctggggtgc ctatgcggaa acaatgaagc tgattctatc cggtggagaa aagatggaca
      541 ttgtaccaat cctggtggaa caggttaatt ccatggtgaa tgcgaagcag gttattgaca
      601 tgtctgaata tatcgacaaa tatggcaata atattaaaga actgcttggt gatactgcga
      661 aagcagcaaa tatcggaaat tatgtatacg gtgtgacaac aggacgcgaa tggttctgcc
      721 agtcatctgt tattatgcga aaagatattc tggatgagtg tggaattgat gtatcttcta
      781 tcacagatta taaagatttg actgacgttt atgcaactgt gaaagaaaaa tatccagata
      841 tggtcatgat ggcaagcaat aacagtgcaa caccggatac caaatatgaa atgaatgata
      901 cacttactga tggatttggt gtattgatgg atcatggtca ggatacgaca gttgtagatt
      961 attatgagac agatgaatat aaagagtttg tagaaacaat gtatgactgg cagcaaaaag
     1021 gttatttgtc caaagatgct gcaaccacaa cagagagtgc ggagaatcag gtaaaagcag
     1081 gtgccgcttt ttcttacctt gcaccaaaca aaccaggata tgatacccgt gccgctttac
     1141 tttgcggaac agagatggag attgctccga tttccgaacc atgggctggt actgcacaaa
     1201 tttcttacct gacttatggc atcagttcaa gttctgctga taaagataaa acaatgcagt
     1261 gtctggatta tttatatgga aatgcagata ttctgaacct gttgaactgg ggggaagaag
     1321 gtgttgatta cgaagttgta gatgctgaaa ataatatcat taactatccg gatggaaaag
     1381 atgacagcaa cacgtatcat ctggcagaag gatggcagtt atttgaccag tttaaaatgc
     1441 atatctggga gggtgattca ccagatatct gggatgaaac aaaggcactg aatgagagtg
     1501 caatcaaatc aaaagcattc ggatttacct atgattctac aagtgttgca aacgaactgg
     1561 ctgcactgag taatgtaaaa gcaaaatatg cagctgctct tggttctggt acagtagatc
     1621 ctgaagaaac acttccgaaa tttattgagg aactgaagaa agcaggaatt gagaaagtga
     1681 tcagcaccaa acaggaacag ctggacaagt ggctggaaga gaataaataa cacagacagg
     1741 ggatgccgct atgtatcccc tgttatattc cagataattg aaaggaaaaa tttatgaaaa
     1801 aagcaaaatt taaaagatgg gtaccgcttt atctgatgat ggcaccgggg ctgatttatc
     1861 tttttatcaa taattatatg ccgatggcag gacttgtggt tgctttcaaa aattacaacg
     1921 tggtagatgg aatctttggc agcccatggg ccggtctcag caattttact tatctgttta
     1981 atgatgcgtg gacgattaca agaaatacgt tgctttataa tattgtattt attatcatca
     2041 acctgattct gggaattgca tttgccatct ttatctgtga tattcggagc aaggcatgca
     2101 aaacaattta tcaaagtgca attttactgc cgtttcttat gtctattgta attgtaagtt
     2161 atatcacttt tgcctttttc agtggggata atggaatgct gaataagaca attcttccgt
     2221 ttttcgggaa agaagcaatt aattggtatt cagaatccaa atattggcct gtgatcctgg
     2281 ttatcgtaaa tacatggaaa ggtgtaggat atggatgcct gatttatatt tccagtattt
     2341 ccggaattga tccttctttt tatgaggctg cagaactgga tggagcttca aagtggaaac
     2401 agatccgata cattacactt ccatcgatca tgccgtctgt aatcactttg acactgttaa
     2461 acattggaag aattttctat tctgattttg gattattcta tcaggttaca caaaattccg
     2521 gtcagcttta tgataccacc aatgtaatcg atacttatgt atacagggca ctgcttcagt
     2581 ccggaaatat cggaatggct tcagcggcag gcttttatca gtccattgtc ggatttgcct
     2641 gtgtactgct ggcaaatgtg gttgtgcgca aattaagccc tgaaaatgca atgttctagg
     2701 aggtaaaaaa tggttagaag caaaagtgca aaaagatttg aacgattggc acatacggtc
     2761 atgatactgg ttacaatctg tattgtattg ccgtttatct tgttatttat gtcatctatt
     2821 acctctgaaa gtgctctggt tagagatgga tattcctttt ttccaaaaga gttcagtatt
     2881 catgcctata agtttatctg ggataatgct gcaaatgtat tcagggcata tggaattaca
     2941 atactggtca ctgtaatagg aaccagtatt aatgtggcaa tgtctgcatt gttggcgtat
     3001 ccgctttccc tgaaaaatct tccaggaaaa agaatcctga atttctacat attttttacc
     3061 atgttattta atggtggact ggttcctaca tatctcatgt atacaggaat tttccatatc
     3121 aataacacac tgcttgcata tattgtgccg ggacttctga tgagtgctat gaatgttatg
     3181 ctgattcgta cattctttgc gaccagtatt ccggatgcac tttttgaagc agctcagatt
     3241 gacggtgcaa gtcaattcca gatttttttc aaaattgtat tgccactggg taaacccatt
     3301 cttattgcca tgggtctgct ttccggatta ggatactgga atgactggac aaatggtctg
     3361 tactatatta gagatacgaa gttatatggc attcagaacc ttctgaataa gatgatttca
     3421 gatttgacag cactgacaca gaatgcttct ggtgcgtcaa ctgttgcaat tgcagatatt
     3481 ccatctgcat ctgtcagaat ggcaatagcg tttgttgcaa tgctaccgat tttatgcctg
     3541 tatccgtttt tacagagata ttttaccaaa ggtatcgcgc ttggtgccgt aaaaggctga
     3601 agatgttttg aaggaaaggt ggattatttt cctgtaaata tttagtgttt tcagcaaaga
     3661 ttttttctga taagatgggg aatagaaagt attggggaaa aacggtcgga acgatcattg
     3721 catcgcttga gagatgttgt tctcagaaag aaaggataaa acatgaacga ttataaattg
     3781 gatttagaga aatatgcaac actggcgaga caggcagccg cagagggatg tgtacttctg
     3841 gagaatgaga agcaggcact tcctttgaga gagggagaga gtgtggctgt ttttggccgc
     3901 atggcatttc attattataa aagcggactt ggttccggcg gtctggtgaa tacgagatat
     3961 gtagtgggaa ttctggatgc gctgaaggag tgtaaagaga ttcaactgga tgagaaactg
     4021 ctgggtattt atgcaaactg gattaaagag aatccttatg atgaaggtca gggatggggt
     4081 cgtgttccgt ggtctcagaa agagatggaa gttacagagg aaatgctgga ctgcgcacgt
     4141 agcaacgatg tttcgcttgt tattatagga agaactgcag gtgaagatca ggataataat
     4201 acgaatctgg gaagctattg tctgacggag acggaggagg atttgatctg ccgggtctgt
     4261 gaggtgagta agtgcacagt tgtggtgctg aatgttggaa atattattga tatgagttgg
     4321 gtggagaaat atcatcctca ggctgtgctg tatgcatggc agggtggtca ggaaggtggc
     4381 aatggtgtgg ctgatgtgct gaccggtaaa gtgtgtgcat gtgggaagct gacagatacc
     4441 attgctgaga ggattgagta ttatccgtct acggagaatt ttggtgatcc atataagaat
     4501 tattataagg aagatattta tgtgggttat cgttattttg agacatttgc gaaggataag
     4561 gtgctgtatc cgtttggata tggtttgtcc tatacgaatt ttgagacaaa ggcggagatt
     4621 tttaagaata ctgaggatga actcacagtg gctgcgacgg ttaccaatat cggtgatgtg
     4681 aggggcaaag aggttgtaca ggtttatgta aaagcaccgc agggtaaact tggaaatccg
     4741 gcaagaaagc tgatcggatt tgcaaaaacc cgggaactgg caccgggtga aaaagaagaa
     4801 cttgttatca tcattcccaa atatgatatg gcctcttatg atgacagtgg agtgacagga
     4861 cacaaatcct gttatgttct ggaagaggga acttatgaaa tttttgcagg cagtgatgta
     4921 agaagtgcga aatcagcagg aatttnnnnn nnnnngtaat agaacagtta caggaggcat
     4981 atgcaccgat tgagaaattt cggagaatga aagccgttct gagagcggat ggaacttatc
     5041 aggcagtaac agaagaggtt cctgtccgca cagcagatcc acataaacgc agagaggaaa
     5101 gaatgccgaa gactctggag tatacgggag ataaaggcta taagctggca gatgtactgg
     5161 ataaaaaagt ttctatggat gagtttgtgg cacagatcag tgaggcggat ctgattgcca
     5221 tgttccgcgg tgaaggaatg tgcagtccaa aggtcactgc tggaactgca gctgcatttg
     5281 gcggtgtgac agagtctttg aaggctcttg gaatcccggt gggatgctgc gcggatgggc
     5341 cgtcagggat tcgtatggat tgcggtacga aagcgttttc tctgccaaat ggaactttgc
     5401 tgggctgtac ttttaatacg gagcttgtgg gtgagctgta tgaaatgaca gggagggaac
     5461 ttcgactgaa taagattgat tccctgctgg gaccaggtat gaatattcac aggaatccac
     5521 taaatggtcg aaattttgaa tatatttctg aggatccgct tctcacagga agaatttgtg
     5581 cggctcaggt gaaggcaatg gctaaatccg aaattggaag tacgattaag catttctgtg
     5641 gaaataatca ggaagtggga cgaagtactt ctgattctgt tatgtctgag agatgtctga
     5701 gagagatcta tctgaaggga tttgagattg cagtaaagga gggtggtgca cgttctgtaa
     5761 tgactactta tggaagtgta aatgggctct ggactgcagg tagctatgat ctgtgtacaa
     5821 ctattctgag aaaagagtgg ggatttcagg gtattgtcat gactgactgg tgggcgaagt
     5881 ccaattatga gggtcatcag gcagaggtaa ctgcgaaagc tcctatggta gctgcccaga
     5941 atgatattta tatggtagtg agtgatgcga agtccaatcc ggagaatgac gatgtggagg
     6001 aaatgcttca tgcaggaaaa attactgtgg gagaattgca gaggaatgct gccaatatcc
     6061 tgggatttct gttaaaaagt ccttctgttt tattgttgac agacagaata tgtaaagaag
     6121 agctggaggc tatgaataca aaggaagagg acgatgtgga tgccggaagt ctggtaagta
     6181 ttgaaagtga ttcggtgaca cagaaaattg taattgacgg tgcattatta catcctgcaa
     6241 agggaaaggc agatgtgatt gctgtgacta atgagttcat gggtgatttc acaatgaagt
     6301 ttactttgaa atctgatctt ggtgagctgg ctcagcttcc ggtttctgta tttttggata
     6361 atattcataa gatgactgtt tcagtacagg ggaccaatgg aaaatgggta gaagaaagcc
     6421 gcatcctgaa tatggaattt ggtcataatc actatatcaa attttattat ggtgcggata
     6481 attttgaaat taaagaaatt gtgctgattc cgaataggta agaccaattt tgggaacgct
     6541 tgcaaaacta tatcctttat ggcataatta tgaaaatact cagaacctgc gaaagatgag
     6601 tgataattgc tttataaaag tggttatctg tggacttttt taatgcggag cgggctgttc
     6661 agaggaaaag tgttactgat gattagaaca gtgttaagag tgatgaggaa aggataa
//
LOCUS       DBMDXG010000070         6644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2951_length_6644_cov_2.184472, whole genome shotgun sequence.
ACCESSION   DBMDXG010000070 DBMDXG010000000
VERSION     DBMDXG010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2951_length_6644_cov_2.184472"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      216..409
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            607..1545
                     /gene="nikB"
                     /locus_tag="ACZPD3_03295"
     CDS             607..1545
                     /gene="nikB"
                     /locus_tag="ACZPD3_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HIR1415064.1"
                     /translation="MTKKQIGKRFLQILIVLFGISFLTFGLTYLSPGDPAEIMLTECG
                     NLPTPELLEQTRHELGLDQPFLVQYGTWLKGVLTGDMGQSYSFKMPVVQKLVSCFWPT
                     LKMSLLALLMMIVISIPLGILAAVYQNKFPDYFVRGITFIGVSVPSFWLGLILLSIFG
                     VQLRWVNVAGGSTDFKAMVLPALTLALAMSAKYTRQVRSAVLEELHQDYVTGARMRGI
                     KERVILWRHVLPNVMLPLVTLLGLSLGSLLGGTAVVEIIYNWPGMGNMAVKAISCRDY
                     PLVQGYVLWIAILYMCINLLVDLSYTYLDPRLKEDR"
     gene            1546..2370
                     /gene="nikC"
                     /locus_tag="ACZPD3_03300"
     CDS             1546..2370
                     /gene="nikC"
                     /locus_tag="ACZPD3_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HIR1415065.1"
                     /translation="MKLAKVFKENKQFTLFSILALVVVGVAVFAPQIAPYDPLDAVML
                     ESLQAPSSAHPFGTDKLGRDLLSRVIFGTRTSLIMTLCLVAVIFVIGTFLGVIAGYFG
                     GIVDGVIMRIADMMISFPGLVLAIAIAGMLGPNLVNAVISIAAVSWTKYARLARSMVL
                     RVKKNLYIEAAVVNGTRTWKILLVHVLPNMVTQMVVTAAADIGTMMLELASLSFLGFG
                     ATAPTPEWGLMLNEGRTYMAKAPWLMIYPGIAIVICVVIFNMLGDSIRDVLDPRQE"
     gene            2467..4023
                     /locus_tag="ACZPD3_03305"
     CDS             2467..4023
                     /locus_tag="ACZPD3_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1415066.1"
                     /translation="MKNTLFKKVLAVALACTMTFSMATVTHAADAKEITIGVTSFADT
                     LEPTEQYFSWVVSRYAIGECLTKFDEEGEIVPCLAESWENSEDGKTWTFKIREGVKFS
                     NGDDMTPEMVMASIQRTVDKSDRVPEFFDLDSMEVDGQNLIFHLNRANANMAGSLADP
                     LFLIVDTNVDDSTFAMEGPICTGPYKVESFSPTDSCVVTKNENYWDGEVPLDKVTLKC
                     VDDQTTRSMALQTGEIDIAYNLKTENLIEFEGNDNYEIQELQSLRSTYAFMNQSEGRA
                     LSDKALRQALLRGLDKQTYCDVLLEGGATAGKAPVPPTLDFGFDDLNDENSYDPEGAK
                     AILEEAGYKDTDGDGFVETPDGDPLTLDFVIYTSRAELGVYAQAAQASLKEIGINVNL
                     NTVSYETLLDMRDSGQYDLLIWNVLVANTGDPENYLRENWYSKSANNTAGYNNPEVDE
                     LLDELAGTFDEEERKDLIIKIQQDIMDDAATVFFGYETTYLFSNKRVTGVKMYPMDYY
                     WLTKDITLAE"
     gene            4150..4935
                     /locus_tag="ACZPD3_03310"
     CDS             4150..4935
                     /locus_tag="ACZPD3_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415067.1"
                     /translation="MLEIKDLSVRYGKQAPTIEHFNLNMKKGEIISVVGESGSGKTTV
                     IRAVLGALAGGGKVTQGDILFNGKSLLTNTKDEWRQLRGTRISMIFQDCGGTLNPIRK
                     IGSQFVEYICTHSKVSKDEAWKKGVSMLEKMRLPEAENIMNSYPHQLSGGMRQRVGIA
                     MAMTFNPELLLADEPTSALDVTTQAQIVRQMMELRDDFHTGIIIVTHNIGVAAYMADQ
                     LIVMQNGKVVDQGTRDEVMNHPTSDYTKKLLAAVPEMEGRRFV"
     gene            4928..5899
                     /locus_tag="ACZPD3_03315"
     CDS             4928..5899
                     /locus_tag="ACZPD3_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415068.1"
                     /translation="MFKESDIVLSAKHIVKQFPASHGRTLTACNDINLNVYKGKTLGI
                     VGESGCGKSTFVRMVISLDKPTSGEILYHGKNLADLSKKETWLNRQNMQMVFQDPLAS
                     FDPKMKIVDILTEPLINFGKLKKSEKEAKARELLEMVELPGDFVDRYPHNMSGGQRQR
                     ISIARALSLEPEILVCDEATSALDVSVQESVIELLVRLQKEKNISMLFICHDLALIRS
                     FAHQIAVMYLGNIVEVIPGEDVTEHAVHPYTQALLGAQFSIHMDPSQKIQSIESEAPS
                     PLDVPKGCPFQNRCEHCTDRCKAEMPKLKEIAPGHAVACFYVEEQKI"
     gene            complement(5962..6444)
                     /locus_tag="ACZPD3_03320"
     CDS             complement(5962..6444)
                     /locus_tag="ACZPD3_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HIR1415069.1"
                     /translation="MDLIKEALSMGFADAAIMDTKDLVFVPEYRQFCEDNLCGNYNLV
                     PACPPACGTVEEMQAKALKYEKALVLQTVLKDPIMDPALFKQAKHAQNILTEQLARQM
                     QEAGKEDILIMSAGPYKNCSCMSAYSVDAQKMADAVGMVCWADDSDVRFFTQILFHKD
                     "
BASE COUNT         1936 a         1341 c         1597 g         1770 t
ORIGIN      
        1 ttcaatgaag ggaacttata acgaagggaa aaattatctt tttagcaaat aatattgaag
       61 aataaaaatg attcgtttat ctgttacagt aaaaggaata aaaaaatcgg attgtatgtt
      121 tttgaataca atctgatttt ttttgctaat tactggacaa agtaaaaaaa actattctat
      181 aatattatag taaaaattaa ataacattta ttggttttga accagtcgtt gtaagttccg
      241 caaggattaa tagagaaacc ggtgtaagtc cggtacgatc ccgtcactgt aagagggagc
      301 ttcggaatga aaaatgtcac tggaccatgt gattttaaag ttggtccggg aaggcgattt
      361 cgaagtgatg aacttaagtc aggagaactg cttgcaattg ggataggaat gattcttacg
      421 gtagatagga agcataattt tttcatgtca ttttgtgtcc gtatgtatga tgttttcata
      481 ttgcgggcta tttttttgcc tgaaaattaa cccctatggg gggattcccc atgttactga
      541 gtaaaagtaa tcagaaacat ttccggtatt ggtttaattc acgaaagatg taggaaggag
      601 attattttga cgaaaaaaca aatcgggaaa agatttctgc agattctgat cgtcctgttt
      661 ggtatcagtt ttctgacctt tggactgact tatctgtctc ctggtgatcc ggctgagatc
      721 atgctgacag agtgcggtaa tcttcccaca ccagagcttc tggaacagac aaggcatgag
      781 ctgggacttg accagccctt tcttgtacag tatggaacct ggctgaaggg tgtactcacc
      841 ggagatatgg gacagtccta ttcctttaag atgccggtgg tacagaaact tgtatcctgt
      901 ttctggccaa ccctgaagat gtctctgctg gcactgctta tgatgatcgt gatttccatt
      961 ccgctgggaa tcctggcagc agtgtatcag aacaaattcc cggattactt tgtaagagga
     1021 attactttta tcggggtatc cgtaccaagc ttctggcttg gcctgatctt actgagtatt
     1081 ttcggtgtac agcttcgctg ggtgaatgta gcaggaggaa gcactgattt caaagcgatg
     1141 gtcctgccgg cacttacgtt ggctcttgcc atgtctgcaa aatataccag acaggtaaga
     1201 agtgcggttc tggaggaact tcatcaggat tatgtaacag gtgcacgtat gagaggtatt
     1261 aaggagcgtg tgattttatg gagacatgtt cttccaaatg taatgcttcc gttggtaact
     1321 ctgcttggac tttccctggg aagtcttctt ggcggtactg cagtcgttga gattatctac
     1381 aactggccgg gtatgggaaa tatggcagtc aaagcaatct cctgccgtga ttatccgttg
     1441 gttcagggat atgtactgtg gatcgctatc ctgtatatgt gtatcaatct gcttgtggat
     1501 ctgtcctata cttatctgga tccaagactt aaggaggata gataattgaa gttagcgaaa
     1561 gtatttaaag aaaataagca gtttaccctt ttttcgatac tggcgttggt cgtagtcggt
     1621 gtggctgtgt ttgccccaca gatcgcaccc tacgacccgc tggatgcagt gatgctggaa
     1681 tcacttcagg ctccaagttc tgcacatccc tttgggacag ataagctggg acgtgatctg
     1741 ttatcacgag ttatttttgg aaccagaact tctctgatta tgaccttatg tctggtggca
     1801 gttatctttg tgatcggtac atttcttgga gtgatcgccg gatacttcgg aggaatcgtg
     1861 gacggtgtga tcatgcgtat cgcagatatg atgatctctt tccccggtct ggtgcttgca
     1921 atcgcaatcg ccggtatgct gggaccaaac cttgtgaatg ccgttatctc cattgcagcg
     1981 gtaagttgga ccaaatatgc ccgtcttgca cgaagcatgg tactgagagt aaagaagaac
     2041 ctttatattg aagcagcagt cgtaaacgga accaggacat ggaagatcct gcttgtacat
     2101 gttcttccga acatggttac tcagatggta gtaactgcag cagcagatat cggaaccatg
     2161 atgctggaac tggcatcctt atccttcctt ggttttggtg ctacagcgcc gacaccggaa
     2221 tggggactga tgctgaacga gggaagaact tacatggcaa aagcaccatg gctgatgatc
     2281 tacccaggta tcgcgattgt aatctgcgta gttatcttca acatgcttgg tgacagcatc
     2341 cgtgacgttc ttgacccaag acaggagtaa gaaaatataa aataagaaaa taaaaacaaa
     2401 gagttctgcg gcttccgtac tgaaccgtgg acttcaccac ttacctaaat atttcaggag
     2461 gaatatatga agaatacatt atttaaaaaa gttttagcag tagcacttgc atgtacaatg
     2521 acattttcca tggcaaccgt aactcacgca gcagatgcaa aagagatcac aatcggtgta
     2581 acaagctttg ctgatactct ggagcctaca gagcagtact tcagctgggt tgtttcccgt
     2641 tatgctatcg gtgagtgcct taccaaattc gatgaagaag gtgagatcgt tccttgtctg
     2701 gcagaaagct gggagaacag cgaagacgga aaaacatgga cattcaagat tcgtgaaggc
     2761 gttaaattct ccaatggtga tgatatgaca ccggagatgg taatggcatc tattcagcgt
     2821 acagttgaca agagtgaccg agtaccggag ttctttgatc tggacagcat ggaagtagac
     2881 ggacagaacc tgatcttcca cttaaacaga gcaaacgcaa acatggcagg aagccttgca
     2941 gacccattat tcctgatcgt agatacaaac gtagatgatt ctacatttgc aatggaagga
     3001 ccgatctgta caggaccata taaggtagaa tctttcagcc ctacagattc ctgtgtagtt
     3061 acaaaaaatg agaattactg ggatggagag gtgccgctgg ataaggttac actgaaatgt
     3121 gtagatgatc agacaacacg ttccatggct cttcagacag gcgagatcga tattgcttat
     3181 aacttaaaga cagagaacct tattgagttt gaaggaaatg acaattatga gatccaggag
     3241 cttcagtccc tgcgttccac atatgcattc atgaaccaga gtgaaggccg tgcactcagc
     3301 gataaagcgc ttcgtcaggc acttctgcgc ggacttgaca aacagacata ctgtgacgtt
     3361 cttcttgagg gtggagctac agcaggtaaa gcgccggtac ctcccacact tgacttcgga
     3421 tttgatgatc tgaacgatga gaacagctat gatccggaag gtgcgaaagc aatccttgag
     3481 gaagcaggat acaaagatac agacggtgac ggattcgtgg aaacaccgga cggagatcct
     3541 ctgacacttg atttcgttat ctatacaagc cgtgcagagc ttggtgttta cgcacaggca
     3601 gcacaggcca gcctgaaaga gattggtatc aatgtaaacc tgaatacagt aagctatgaa
     3661 acacttctcg acatgagaga ttccggtcag tatgaccttc ttatctggaa cgtacttgta
     3721 gctaatacag gagatccgga gaactattta agagagaact ggtacagcaa atctgcaaac
     3781 aacacagcag gatacaacaa tccggaagta gatgaacttc ttgatgagct tgcaggaaca
     3841 tttgacgaag aagaaagaaa agacctgatc atcaagatcc agcaggatat catggatgat
     3901 gcagcaactg tattcttcgg atatgagaca acttatctgt tctccaacaa gagagtcaca
     3961 ggcgtgaaga tgtatccgat ggattactac tggctgacaa aagacatcac acttgctgag
     4021 taattatatt cagagatatt aatttctgaa tgagactgca cggattatgt ctgttcatcg
     4081 ggatatcaga aaagatggac acagatataa tccaaaaaac acagcaacaa gtaagaagga
     4141 gcgttattta tgcttgaaat aaaggatctc tcagtccggt atggaaagca ggcacctact
     4201 atcgagcatt tcaacctgaa catgaagaag ggcgagatca tcagtgttgt aggtgagagt
     4261 ggaagtggaa aaaccactgt tatcagagct gtcctcggcg cacttgccgg gggcggtaag
     4321 gtaacacagg gagatatcct ctttaacgga aaatccctgc tgaccaatac caaggatgaa
     4381 tggagacagt taagaggaac acgtatatcc atgatcttcc aggactgcgg cggaaccctg
     4441 aatccgatcc gtaagattgg aagccagttc gtggaataca tctgtactca ttcaaaagtg
     4501 tcaaaagatg aagcgtggaa aaaaggagta agcatgctgg agaaaatgcg tctcccggaa
     4561 gcagagaata ttatgaacag ttatcctcat cagctttccg gtggtatgcg tcagcgtgtg
     4621 ggtattgcca tggcaatgac ctttaatcct gaactgcttc tggcagacga accaaccagt
     4681 gcactggatg tgaccacaca ggcgcagatc gtccgtcaga tgatggaact gcgtgacgat
     4741 ttccacacag gcatcatcat cgtaacccat aacattggtg tagctgctta catggcagac
     4801 cagctgatcg ttatgcagaa cggcaaagtg gtggatcagg gaacaaggga tgaggtcatg
     4861 aatcatccga ccagtgatta tacaaagaag cttctggcag cagtaccgga aatggaggga
     4921 cgccgttttg tttaaggaat cagatatcgt actcagtgca aagcacattg taaaacagtt
     4981 tccggcctct catggacgga cactgacagc ctgcaatgac attaacctga atgtctataa
     5041 gggcaagaca ttaggaattg taggagagag cggatgtggt aaatccacat tcgtaaggat
     5101 ggttatttcc cttgacaagc ctaccagcgg tgagattctg tatcatggca aaaaccttgc
     5161 tgacctttcc aaaaaggaaa catggttaaa ccgccagaat atgcagatgg ttttccagga
     5221 tccgctggca tcctttgatc cgaagatgaa gattgtggat atccttacag agccgctgat
     5281 caacttcgga aaattaaaga aaagcgagaa agaagccaaa gcaagagaac tgctggaaat
     5341 ggtagagctg ccgggagatt tcgtagatcg ttatcctcac aacatgagtg gcggacagag
     5401 acagagaatc agtattgcaa gagcgctttc actggaaccg gagatcctgg tttgcgatga
     5461 ggcgacttct gcgctggatg tttctgtgca ggagtctgtg atcgagcttc tggtacgcct
     5521 tcagaaagaa aaaaatatca gcatgctttt catctgccat gacctggcgc tgatccgctc
     5581 ttttgcccat cagattgctg tcatgtatct gggaaatatc gtagaggtta ttcccggtga
     5641 agatgttaca gagcatgcag tgcatccgta tacacaggca ctcctgggtg cgcaattctc
     5701 tatccacatg gatcccagcc agaagatcca aagtatcgag agtgaagcac caagtcctct
     5761 ggatgtaccc aagggttgtc ctttccagaa ccgctgtgag cattgcacag acagatgtaa
     5821 ggcagagatg ccgaagctga aagagattgc tcccggacat gcagtggcat gcttttatgt
     5881 ggaagaacaa aagatataaa aatgaaaaag aggctgtcac atcaggaatt ttccggtgcg
     5941 acagtctctt ttgcgtaatg ctcagtcttt atgaaacagg atttgtgtaa agaatctcac
     6001 atctgaatca tctgcccagc agaccatacc tactgcatca gccattttct gtgcatctac
     6061 actgtaggcg gacatacagg aacaattctt atacggtcct gcgctcatga tcaggatatc
     6121 ttcttttcct gcttcctgca tctgtctggc aagctgttcc gtaagaatat tctgtgcatg
     6181 tttcgcctgc ttgaaaagag ccggatccat aattggatct ttcagaacag tctggagaac
     6241 cagtgccttt tcatatttca atgcttttgc ctgcatttct tctacagtac cgcaggcagg
     6301 cggacatgca ggaaccaggt tgtaatttcc acagagattg tcctcgcaga actgtctgta
     6361 ttccggcaca aataccagat cctttgtatc catgatcgcc gcatctgcga aacccatgga
     6421 aagtgcttct tttattaaat ccataagaat gtctcccttc ttaaagcgga ttatttactt
     6481 tttatctttt acgattataa cattttgaag taaataaaaa cagaaaaatc aaaataaaat
     6541 atattactgt tcactccgtt cacagtaacg tagccaaaat tcattccaga ttgcctgcgg
     6601 caatggaatt ttggcttgta tgtctcggga ttttggcata ttca
//
LOCUS       DBMDXG010000071         6609 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_2970_length_6609_cov_1.614321, whole genome shotgun sequence.
ACCESSION   DBMDXG010000071 DBMDXG010000000
VERSION     DBMDXG010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2970_length_6609_cov_1.614321"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="ACZPD3_03325"
     CDS             complement(<1..1496)
                     /locus_tag="ACZPD3_03325"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1415070.1"
                     /translation="MGEVILTMKDIDKSFPGVHALDHVNFEVKRGEVHALMGENGAGK
                     STLMKVLTGIYQKDSGSITYKGKETEFHNTREAQDAGVVIVHQELNMVGDLTVAQNIF
                     IGREPKKGFSIDDKKMIEDSKKLFQELNIEINPKEKMNNLTVGKQQMCEIAKAISHKA
                     EVIIFDEPSAALTEKEIADLFEIIRDLRKKGLGIVYISHRMDEIKTITDRVTVMRDGG
                     YVGTLITADSTKEDIINMMVGRVIYEDPKEHSMVAPDAPVVLKVENLNAGKMVQNVSF
                     ELRKGEILGFSGLMGAGRTETARALFGADPKQSGKISIRGKDGQLREVTINSPQDAVK
                     YGIGYLSEDRRRYGCVVQKSVTENTTLATMEEFTSGIFINKSKEKEVSEKYVKELATK
                     TPNCEQLVVNLSGGNQQKVVIAKWLTRDSEILIFDEPTRGIDVGAKNEIYKLMNRLAA
                     EGKSIIMISSEMTEVLRMSDRIIVMCEGKITGNIDISEATQEHIMNHAT"
     gene            complement(1517..2494)
                     /locus_tag="ACZPD3_03330"
     CDS             complement(1517..2494)
                     /locus_tag="ACZPD3_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415071.1"
                     /translation="MEKLKQNPIVKKLGLNRILLACILVLMFVVFKVVLGSKFPVGDS
                     IKSTLNYVYFLGFLSLGVTFVIATGGIDFSIGPVMFCCALISGYCMTSYHVPCAAAMV
                     ICILIGFAFGVFNGWMVSYMSVPPFIISMASMNIAKGIASVFTKTQSVSWPLGSDPVN
                     GWFRNLISYKGFPVGLVIFLAAAVICGIILYNTKPGRYILCLGSNSEAVRLSGVNTKK
                     WRMLAYVICGVLVGIGAIFFVGAYTTVQPGYGDQYNNEAIAGCVMGGTSMVGGLASIG
                     GTVIGVFIISLLQQGIMAFGLGKGQQMIITGLIVIVAVYVDVSARRRKN"
     gene            complement(2679..3656)
                     /locus_tag="ACZPD3_03335"
     CDS             complement(2679..3656)
                     /locus_tag="ACZPD3_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1415072.1"
                     /translation="MKSHKKEIITVAILMAAAVVIFAGILKPEATQTKKCSLIYIPKI
                     RDNTNDFWTSVISGCKMAAEEYESDLEILAPDKEENIEEQNKLLKKAIEQKPDAILFS
                     PSSMDASDELLKEAKEKGIRITYIDSYTKEKLQDLTVATDNVNAGRMLGEYARKLIDK
                     DSKIAIVSHVKGVSTAVEREQGFREGLGDYADNIVDIVYCNSLYEKSYELAQELMRKY
                     PDLELIAGMNEYSAVGVGRAVSDAGAKDKIAVVGVDCSQEAINLMEMGVYKGIIVQKA
                     FRMGYIGVEETIHMLNGDAVEKNIDSGCELVTPENMYNSDIERLIFPFS"
     gene            complement(3643..5448)
                     /locus_tag="ACZPD3_03340"
     CDS             complement(3643..5448)
                     /locus_tag="ACZPD3_03340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415073.1"
                     /translation="MKISSKFKSIQSSIFCAVSILVLSAVLVVTVVSLRYTNSSIYEN
                     SVMYTQTIIKQLNQNIDSYISYMDNIASVIAQSGDAYKYLYSEKGHGATKDENYSEYR
                     QRLVEQFKTILKGRADIRNIGIVREDKNSPSLFDNGLSVRNTYVDLNTQPWYADAVGK
                     YDRYNLTSSHVQNVIKGERPWVITLSRGIRNYTGTEAEDGVVFLDLNYSAISELCAQS
                     SMGDKGYVFILDQNGNIVYHPQQQQLYNELQTENISLVMNAKSDIVTVGKGDDEKIYA
                     LSHSDITGWTIVGCMNMSELLRNSRQTRSIYVLVAVGLIIVALLISSLIARNITLPIQ
                     KLRDSMKSVQKGNFDIEDIEVISDNEIGSLTRSFNVMTHRIRELMEQNVKEQEQKRKI
                     ELKALQSQINPHFLYNTLDSIIWMAEGKKNEEVVIMTASLARLLRQSISNEDELVTVG
                     QEIEYVRSYLTIQKMRYKDKLEFEIKADPSITQVPIIRLVLQPLVENAIYHGLKYKDS
                     KGLLTVHGYMKGENAVIDITDDGVGMDEETLKHIYDKHKVNYRSNGVGVYNVQQRLVL
                     YYGKDYGIIYHSEKGKGTTASVVIPGIQEESHEKS"
     gene            complement(5445..>6609)
                     /locus_tag="ACZPD3_03345"
     CDS             complement(5445..>6609)
                     /locus_tag="ACZPD3_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415074.1"
                     /translation="KALETLVNCTRDVQVSLLELKKLGISFDCSSYRVAVFDMDTYSE
                     MYQVDMHKQQESALMSFVLFNISNEIVTRENAGVAYQEGSNRVCIIFTGCRSREFGDK
                     IHSICQEIQQKVKEVIGIETSIGIGSWVRSPQELVYSYKLAEKAIGYRYLLGGSLLLD
                     MEEKKTDNSINLIKSLETLTEEIKVGNRQRVTEILEQIEHEIKGALVEKSYACIYLQQ
                     VIRAIGNTCQSLSDDPEKIIAQREKLLKEVSQAKTFDKAVTLVKEYAEGVFESLQDLN
                     SSSGQRQGMMAMDYIRKNYMDPDLSLNSICSYLNISTSYFSTIFKEMTGETFIESLTR
                     IRMEKARELLENTTLKNYEIAEKVGFSDPHYFGISFKKMTGKTPTEYAREKRR"
BASE COUNT         1739 a         1628 c         1097 g         2145 t
ORIGIN      
        1 gttgcatgat tcataatgtg ttcctgtgta gcttcggaaa tatcaatatt tcctgtaatc
       61 ttaccttcac acataacgat aatacggtca ctcatacgga gaacttctgt catttcagaa
      121 gaaatcatga taatagattt accttctgca gccagtctgt tcattaactt gtaaatttca
      181 ttcttggcac caacatcaat acctctggtc ggctcatcaa aaataagaat ctcactgtct
      241 cgtgtcagcc attttgcgat aacgaccttc tgctggttac caccggaaag attgactacc
      301 agctgctcgc agtttggagt tttggttgcc agctctttaa cgtatttctc tgaaacctct
      361 ttttcttttg atttattaat gaagatacca ctggtaaatt cttccatggt tgccagcgta
      421 gtattctctg ttacagattt ctgtacgaca caaccatatc ttcttctgtc ctctgacaga
      481 taaccaatac catatttaac tgcatcctga ggactgttga tagttacctc gcgaagctgt
      541 ccgtcttttc cccttataga gatctttcca ctctgtttcg ggtcagcacc aaacagtgct
      601 cttgctgtct cagtacgtcc ggctcccatc agtcctgaga aaccaagaat ctcacctttg
      661 cgaagttcaa agcttacatt ctgaaccatc ttacctgcat tcaggttctc taccttaaga
      721 acaaccggcg catctggtgc aaccatgctg tgttccttag gatcttcata aatgacacgt
      781 ccaaccatca tattgatgat gtcttctttt gtactgtctg ctgtgatcag agttcctaca
      841 taaccaccat ctcgcatgac tgttactctg tcagtgatcg ttttgatctc atccatacgg
      901 tgggaaatat aaacgattcc aagacctttc ttacgaagat cacggataat ctcgaataag
      961 tctgcaatct ctttctctgt cagtgctgct gacggctcat caaagataat aacctctgct
     1021 ttgtgggaaa tagcctttgc gatctcacac atctgctgtt ttccgacggt aagattgttc
     1081 atcttttctt ttggattgat ttcaatgttc agttcctgaa agagtttttt ggaatcttca
     1141 atcattttct tatcatcaat gctgaaccct ttctttggtt cacgtccgat aaaaatattc
     1201 tgagctacgg taagatcacc taccatgttc agctcctgat gcacgattac aacacctgca
     1261 tcctgcgctt ctcttgtgtt atggaattca gtttcctttc ctttataggt aatggatcct
     1321 gaatctttct gataaatacc ggtaagaact ttcatcaatg tagactttcc ggcaccgttt
     1381 tctcccataa gagcatgtac ttcgccgcgt ttcacttcaa aattaacgtg atccagagca
     1441 tgaactccag gaaaggactt atcaatgtcc ttcatagtca aaataacttc acccattgtt
     1501 acatcctccc ttccgatcag ttctttctgc gtcttgcaga aacgtcgaca taaactgcta
     1561 caattacgat aagtcctgtg atgatcatct gctgaccttt accaagacca aatgccatga
     1621 taccctgctg aagaagagaa atgataaata caccgatgac tgtaccgccg atagaagcaa
     1681 gtccgccaac catggatgta ccacccatta cacagcctgc aatcgcttca ttgttgtact
     1741 gatcgccgta tccaggctga acagttgtat aagcaccaac aaagaaaatt gctccgattc
     1801 ctacaagaac accgcagatc acataagcaa gcattctcca tttttttgta ttaacaccgc
     1861 tgagtcttac tgcttcgctg ttagaaccaa gacaaaggat ataacgtccc ggttttgtgt
     1921 tataaagaat aattccacag atcactgctg ctgccaggaa aatcacaagt cctacaggga
     1981 atcccttata ggagataaga tttctgaacc atccgtttac aggatcactt cccagtggcc
     2041 agctgacgga ctgtgtcttt gtaaatacag atgcaatacc ttttgcaatg ttcatagatg
     2101 ccatggaaat gataaatggc ggaactgaca tataagatac catccagcca ttaaacacgc
     2161 caaatgcaaa tccgatcagg atacagatca ccattgctgc tgcacaaggt acatgataag
     2221 atgtcatgca gtaacctgag atcagggcac agcagaacat tacaggtcca atggagaaat
     2281 cgattcctcc agtggcaatt acaaatgtta ctccaagtga cagaaagccc aggaagtata
     2341 catagttcaa agtagatttg atactgtcac ctacagggaa cttacttccc agaactactt
     2401 tgaagactac gaacataaga accagaatac atgctaacag tattctgttc agaccgagtt
     2461 tcttcacaat tggattttgt ttcaactttt ccaagttttt caccctcctt acgttatttc
     2521 gtatctctct ttgttttcta tatcttacct tacctgtcgg ataattgtaa tgaaatatct
     2581 tccaaaaaaa ggttaaatat ttcctttttt tcatccggaa atatttaacc tttttgcttc
     2641 ttttatcctt atattacgca gtcgtttcaa cgcgtcagtc agctaaacgg gaagatcagc
     2701 cgttcaatat cactgttata catattctcc ggcgtaacca gctcgcatcc tgaatcaata
     2761 ttcttctcta ctgcatcccc gttaagcata tgaatggttt cttccactcc gatatacccc
     2821 atccggaatg ctttctgcac aataattccc ttataaacgc ccatttccat aagattgatg
     2881 gcctcctggg aacagtccac accaactact gcgatcttat ctttcgctcc tgcatcactg
     2941 actgctcttc ccacaccaac tgcagaatat tcgttcattc ctgcaatcag ttccagatcc
     3001 ggatatttcc tcatcagttc ctgcgccagt tcataggatt tctcatagag tgagttacag
     3061 tagacaatat ctactatgtt atcagcatag tcaccaagac cttccctgaa tccctgttct
     3121 ctctccacag ccgtggaaac accttttaca tggctgacaa tggctatctt tgaatcttta
     3181 tcgattaatt ttctggcata ctctcctaac atcctgcccg cattcacatt atctgtggca
     3241 actgtgagat cctgcagttt ttcttttgta taggaatcaa tataggtaat acggattcct
     3301 ttttcttttg cttccttcag aagttcatct gatgcatcca tgctggaagg ggaaaacaga
     3361 atcgcatccg gtttctgctc aatggccttt ttcagaagtt tattctgttc ttctatattc
     3421 tcttctttat ccggagccag gatttcaagg tcagattcat attcctctgc cgccatctta
     3481 cagccggata ttaccgaagt ccagaaatca ttggtgttat ccctgatctt tggaatataa
     3541 atgagggagc attttttcgt ctgtgtcgcc tccggtttca ggattccggc aaaaatcacc
     3601 actgctgccg ccataaggat tgcaactgtt ataatctctt ttttatgact tttcatggga
     3661 ttcctcctgt attccgggaa ttacaacaga tgctgtggtt ccctttcctt tttcactgtg
     3721 atagataatg ccataatctt ttccgtaata taaaaccaga cgctgctgca cattgtacac
     3781 cccaactcca ttggaacggt aattcacctt atgcttatca taaatatgtt tcagggtttc
     3841 ctcatccatt cccacaccat cgtccgtaat atcgattact gcattctcac ctttcatata
     3901 tccatgtact gtcaacagtc ctttactgtc tttatatttt aagccatgat aaattgcatt
     3961 ctccacgagc ggctgcaata ccagacgaat gataggtacc tgtgtaatgg atggatctgc
     4021 ctttatctca aattccagct tatccttgta gcgcatcttc tggatcgtga ggtaacttct
     4081 gacatattca atctcctgtc cgactgttac aagctcatcc tcattgctga tactctgtct
     4141 gagcagtcgc gccagagaag ccgtcattat caccacttcc tcattcttct ttccttcagc
     4201 catccagata atagaatcca gcgtattgta cagaaagtgt ggattgatct gcgactgcag
     4261 ggctttcagt tctatttttc tcttctgctc ctgctccttt acattctgct ccattaattc
     4321 ccttatcctg tgtgtcatca cattaaaaga tctggtaaga cttcctattt cattgtcgga
     4381 aatcacctct atatcttcaa tatcaaaatt tcctttctgc acacttttca tggaatcacg
     4441 aagcttctgg atcggcagcg taatatttct ggcaatcagg ctggaaatca gaagagcaac
     4501 tatgatcagc cccactgcta ccagcacata aatacttctt gtctgtctgc tgtttcgtaa
     4561 aagttcagac atattcatgc atcccacaat agtccatcct gtgatatcag aatgggacag
     4621 tgcataaatt ttctcgtcat ctccctttcc gacagtaaca atatctgatt ttgcattcat
     4681 aacaagtgaa atgttctctg tctgcagttc attatataac tgctgctgtt gtggatgata
     4741 aacgatattt ccattctgat caagaataaa aacatatcct ttatctccca tgctgctctg
     4801 tgcacagagt tcactgatcg cactgtaatt cagatccaga aaaactactc catcctctgc
     4861 ttctgttcct gtataattgc ggattcctct gctgagtgtg atgacccatg gtctttctcc
     4921 ctttatcaca ttctgcacat gagaagatgt cagattatac cggtcatatt ttccgactgc
     4981 atctgcatac catggctgtg tattaagatc tacatatgta tttctcacag ataatccatt
     5041 gtcaaacaga gatggagagt tcttatcctc tctgacaatc ccgatatttc tgatatccgc
     5101 cctgcccttc aggatcgttt taaactgttc cacaaggcgc tgtctgtatt cactgtaatt
     5161 ttcatccttt gtggctccat gtcctttttc actgtacaga tatttataag catcaccgct
     5221 ttgcgcgatc actgacgcaa tattatccat ataactgata tacgaatcta tattctgatt
     5281 cagctgtttg atgatcgtct gtgtatacat gacagaattt tcataaatag atgaattcgt
     5341 atatctcaga gataccactg tcacaaccag cacagcactt aataccagga tcgacaccgc
     5401 acagaaaata gaactctgaa tactcttaaa ttttgaagaa atcttcatcg gcgtttctcc
     5461 cttgcatatt cggtaggtgt ttttcctgtc atcttcttaa atgaaattcc gaaatagtgt
     5521 ggatctgaaa atcctacttt ttccgcaatc tcataattct tcagcgttgt attctccaga
     5581 agttccctgg ccttctccat tcttatcctg gtaagtgatt cgataaacgt ttcccctgtc
     5641 atctccttaa aaatagtgct gaaatagctg gtactgatat tcagataaga acagatactg
     5701 ttcagactca gatccggatc catataattt ttccggatat aatccatagc catcattccc
     5761 tgcctctggc cgctggaact attcagatcc tgaagtgatt caaatactcc ttcagcatac
     5821 tccttcacca atgtcactgc tttatcaaag gtttttgcct gagaaacttc tttcagaagc
     5881 ttctctctct gagcaatgat cttttccgga tcatcactca gggactggca ggtatttccg
     5941 atggcacgta tcacctgctg aagataaatg caggcatagc ttttctccac caatgcccct
     6001 ttgatctcat gctcgatctg ctccaggatt tctgtcactc tctgcctgtt tccgaccttt
     6061 atctcttctg tcagtgtttc caaagatttt ataagattaa tactgttgtc cgttttcttt
     6121 tcttccatat ccaaaaggag gctgcctccc agcagatacc gatagccaat ggctttctca
     6181 gcaagcttat aagaatagac cagttcctga ggtgatctca cccagcttcc gattcctata
     6241 gaagtttcaa tcccgatcac ttcttttact ttttgctgga tttcctgaca gatactatgt
     6301 attttgtctc cgaattctct ggatctgcat cctgtaaata taatgcatac ccggttactg
     6361 ccttcctgat aagcaacacc tgcgttctct ctggtcacaa tttcattgct tatattaaaa
     6421 agtacaaagg acatcaatgc gctttcctgc tgtttatgca tatctacctg atacatttct
     6481 gagtaagtgt ccatatcaaa aacagctact ctgtagctcg aacagtcaaa gctgatcccc
     6541 agctttttca gttccagaag acttacctgt acatctctgg tacaatttac aagagtctcc
     6601 agtgctttc
//
LOCUS       DBMDXG010000072         6533 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3006_length_6533_cov_1.643501, whole genome shotgun sequence.
ACCESSION   DBMDXG010000072 DBMDXG010000000
VERSION     DBMDXG010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3006_length_6533_cov_1.643501"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1981)
                     /gene="topA"
                     /locus_tag="ACZPD3_03350"
     CDS             complement(<1..1981)
                     /gene="topA"
                     /locus_tag="ACZPD3_03350"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR1415075.1"
                     /translation="MAKYLVIVESPAKVKTIKKFLGANYDVEASNGHVRDFPKSQFGI
                     DVEHDFEPKYITIRGKGELLAKLRKAAKKADKIYLATDPDREGEAISWHLMQALKEDP
                     KKMHRITFNEITKTAVKSSIKQARDLDMDLVDAQQARRMLDRMVGYTISPLLWAKVKR
                     GLSAGRVQSVALRIICDREDEINAFIPEEYWSLEGDFQVKGEKKPLQAKFYGTDKKMD
                     IHSKEEMDKLLASLKDKEYEINEVKKGERIKNAPLPFTTSTLQQEAAKTLNFSTQKTM
                     RLAQQLYEGIDIKGNGTVGVISYLRTDSTRISEEADAAAREYITAQYGEDYVSKSEKA
                     VKKGQKIQDAHEAIRPTDISRTPAVLKESLSRDQFRLYQLIWRRFAASRMAPAKYETT
                     SVKIGADQYIFTVSASKIIFDGFMSVYTDEEDQKKGNVLNQSLERGMKLSLKELKPEQ
                     HFTQPPAHYTEASLVKTMEELGIGRPSTYAPTITTIISRRYVAKEQKNLYVTELGEVV
                     NNIMKQAFPSIVDVNFTATMEGLLDCVAAGTVKWKTVVSNFYPDLKKDVDAAEEELEK
                     VDIQDEVTDVICENCGRHMVIKYGPHGRFLACPGFPECKNTKPYFEKIGVACPKCGKE
                     IVLKKTKKGRKYYGCEDNPDCDFMSWQKPSAKKCPK"
     gene            complement(2113..3030)
                     /gene="dprA"
                     /locus_tag="ACZPD3_03355"
     CDS             complement(2113..3030)
                     /gene="dprA"
                     /locus_tag="ACZPD3_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1415076.1"
                     /translation="MERTDVKNNHTEEYSTGQIRCIEKSDPEYPQIMKQYASMPAKLY
                     VKGRLPDPKRRTVAVIGARMCSPYGRMQAFRYAKALSVAGVQIISGMALGIDSEGHKG
                     ALEGKMPTFAVLGSGVDVCYPKSNRKLYERILWENGGIISECPLGSGPVSWHFPARNR
                     IISALSDAVLVVEAKENSGSLITAGFALEQGKMVYAIPGAVTDELSRGCHKLIYDGAG
                     IAYCPEIMLEELGISMEKVTQNGEKNNLGLARDLNMVYSCLDLRPKNPDYIVRKTGFS
                     PAQVSNCLVELTLRGLIRESGRHYYVKDS"
     gene            complement(3051..4583)
                     /locus_tag="ACZPD3_03360"
     CDS             complement(3051..4583)
                     /locus_tag="ACZPD3_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1415077.1"
                     /translation="MFASVLSAAIFGVEVCPVQVEADVSNGLPSFIMVGFPSAQVKEA
                     QERVRTALKNNGYQFPPKRITVNFAPADMKKEGAGFDVPVAAAVLAAFEMISPQVVSR
                     VMMAGEIGLDGEIHGISGILPIVLCARSLGSRFCVVPYENLKEGRLIRDVPVAGVKNL
                     RELVECLKNPEPYLKREIQEEIPSIINTDMGMDFSDIEGQEGAKRAAEIAVSGFHNLL
                     LIGPPGTGKTMLARRLPTIMPGLGFEEKLELTRIYSIAGLLSREHPLIDERPFRSPHH
                     TSTPQAIAGGGRNPRPGEITLAHKGVLFLDEMPEFSRASLELLRQPMEDKVIQIARAS
                     GTYNFPADFMLCAAMNPCPCGYYPDLNRCTCTAGEIIHYMGKISRPLLDRIDISTEVP
                     PVSFSQLHCGRKGENSAAIRKRVEKVQKIQEERYKDEKINFNGQLKSSLIDKFCPLTD
                     SASRLLARAFEKIAFSARSYHRILKVARTIADMEGEEMIAGHHIGEALSYRAFDKDSV
                     IK"
     gene            4949..5164
                     /locus_tag="ACZPD3_03365"
     CDS             4949..5164
                     /locus_tag="ACZPD3_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415078.1"
                     /translation="MKFQRIQDLRTDADMSQKQLSEILHISQRSYSHYETGSRNIPVE
                     MLIRLANYYDISVDYLVGRTDKKEMNK"
     gene            complement(5243..5845)
                     /locus_tag="ACZPD3_03370"
     CDS             complement(5243..5845)
                     /locus_tag="ACZPD3_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415079.1"
                     /translation="MSVTNNGKQKRNKKKKRLIKLLIIVIILFILLMLAGTLLLQHRK
                     NVDREQRKAEAIAALERVPTVTPTPAATPTPTPTPIPTATPTPVLERAYVFNPEDYLG
                     TWRSKNGRVKIKIKKLSQKSVTFTYSQTNKKKTATCKAKVKKSVAGNATRFSFTDSLG
                     NVAKGYLTFDNGRLYVNIKTKTKAEGAKVHPSVDTVMIKE"
     gene            complement(5880..>6533)
                     /locus_tag="ACZPD3_03375"
     CDS             complement(5880..>6533)
                     /locus_tag="ACZPD3_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR1415080.1"
                     /translation="EMRYHCGDMLARRDRGEVNPDIVAGVPDSGIAHAIGYANQSGVP
                     FARPFIKYTPTWPRSFMPTQQSQRNLIARMKLIPVHRLIKDKSLLMIDDSIVRGTQLR
                     ETTEFLYRNGAKEVHIRPACPPLLYGCKYLNFSRSKSEMDLITRRVIAKREGENVSDK
                     VLADYADPNSTNYKEMLEEIRKELNFTSLKFHRLDDLKASIGISPCKLCTYCWDGKE"
BASE COUNT         1598 a         1609 c         1236 g         2090 t
ORIGIN      
        1 atttcgggca tttctttgct gatggtttct gccaggacat gaaatcacaa tccggattat
       61 cctcacatcc gtagtactta cgtccttttt tggttttctt aagcacaatc tcttttccac
      121 atttcggaca tgccaccccg atcttctcaa aatatggttt ggtatttttg cattccggaa
      181 atcccggaca tgcaaggaat cgtccatgag gaccatattt gatcaccata tgtctgccac
      241 agttttcgca gatcacatct gtcacttcat cctgaatatc aaccttttca agttcttcct
      301 ctgcagcatc tacatccttc ttcagatccg gatagaagtt gcttactact gtcttccact
      361 ttacagttcc tgctgccaca cagtccagaa gcccttccat agtcgcagtg aagtttacat
      421 ctacaatact cgggaaagcc tgtttcataa tattattgac aacttccccc aactctgtaa
      481 catacaggtt cttctgttct tttgcaacat aacggcggct gatgatcgtt gtgatcgtcg
      541 gcgcataggt acttggacgt ccgatcccca gttcttccat agtcttgacc agagatgcct
      601 ctgtataatg tgcaggcggc tgcgtgaaat gctgttctgg tttcagttcc ttaagactaa
      661 gcttcattcc tctttcaaga ctctgattca gaacatttcc ttttttctgg tcctcttcat
      721 ctgtgtatac agacataaat ccgtcaaata tgatcttgga ggcagagact gtaaaaatat
      781 actggtctgc accaatcttt acagatgttg tttcgtattt tgcaggagcc attctgctgg
      841 ccgcaaaacg tctccagata agctgataca gtctgaactg atctcttgaa agagattcct
      901 tcagtacagc cggtgtacgg ctgatgtctg taggacggat cgcttcgtga gcatcctgaa
      961 tcttctgtcc ctttttcaca gctttctcag actttgaaac ataatcctct ccgtactgag
     1021 cagtgatata ttctcttgca gcagcatctg cttcctctga gattcgggta gaatctgtac
     1081 gcagataaga gatcacacct accgttccat ttcctttgat atcaattcct tcatacagct
     1141 gctgtgccag acgcattgtc ttctgtgtgg aaaagtttaa ggtcttggct gcttcctgct
     1201 gtaaggtact tgtagtaaag ggaagcggtg cattcttgat cctctcgcct ttctttactt
     1261 cattgatctc atattcttta tctttcagag aggccagaag tttatccatt tcctctttgc
     1321 tgtggatatc catcttttta tctgttccat aaaacttcgc ctgcagcggt ttcttctcac
     1381 ccttcacctg aaaatctccc tccaggctcc agtattcttc cggaataaac gcattgatct
     1441 catcttctct gtcacagatg atacgaagcg caacagactg tacgcgcccc gcacttaagc
     1501 ctctcttgac ttttgcccag agaagcgggc tgatggtata acctaccata cggtcaagca
     1561 tacgtcttgc ctgctgtgca tctaccagat ccatatccag gtctctggcc tgtttgattg
     1621 atgattttac tgctgtcttg gtaatctcgt taaaggtaat cctgtgcatc ttcttcggat
     1681 cttcttttaa tgcctgcatc agatgccagg aaattgcctc tccctcgcgg tcagggtcag
     1741 ttgcaagata aatcttatct gctttctttg cagccttgcg aagctttgca agcagttctc
     1801 cctttcctct gatcgttata tatttgggtt caaaatcatg ctcaacatca attccgaact
     1861 gactcttcgg gaaatcacgg acatgtccgt tagaagcttc cacatcgtaa ttcgcaccga
     1921 gaaatttctt tattgttttc acttttgcag gtgattctac tatcaccaga tatttcgcca
     1981 tgctacctct cctagtcgta atctgttatt cttcacagtg tccctgaacg ggcacattgc
     2041 ctgacaacga tattgttaca tatgaaaatc gccctgcgcc gcagaaacgt gaagcacaat
     2101 aatcataaat cgtcaactgt cttttacata ataatgtctc ccgctctctc ttatgagccc
     2161 ccgcagtgtc agttccacaa gacagttgct tacctgtgcc ggggaaaagc ctgtttttct
     2221 cacaatgtag tcgggatttt ttggtcgtaa atcgagacaa ctatacacca tattcaaatc
     2281 tcttgcaagt cccaagttgt ttttttcacc gttttgtgtt actttttcca tcgaaattcc
     2341 aagttcttcc agcataattt ccggacagta tgcaatccct gcgccgtcgt agatcagctt
     2401 atggcagcct ctgctgagtt catctgtcac tgcccccgga atcgcataga ccatctttcc
     2461 ctgttccagt gcaaatcccg cagtgatcag cgaaccgctg ttttcttttg cttccactac
     2521 cagaaccgcg tctgacaaag cactgatgat ccggtttcgc gccgggaaat gccaggaaac
     2581 aggtcctgaa cccagtggac attcactgat gatcccacca ttttcccata agatcctctc
     2641 atagagtttt ctgttagatt tcggatagca tacatcaaca ccacttccaa gcaccgcaaa
     2701 tgtaggcatc tttccttcca gtgcgccttt atgtccttct gaatcaatgc caagtgccat
     2761 accgctgata atctggactc cagctacact taacgctttc gcataccgga atgcctgcat
     2821 ccttccataa ggactgcaca ttctggcacc gatcactgcc accgttcttc tcttaggatc
     2881 aggaagtctg ccttttacat aaagctttgc cggcatggag gcatactgct tcattatctg
     2941 aggatattcc ggatctgatt tctcaataca tcgtatctga cctgtactgt attcttctgt
     3001 atgattattt ttcacatcag ttctttccat ataaaatacc tcactgacct ttattttatg
     3061 acagaatctt tgtcaaatgc cctgtacgaa agtgcttctc cgatatgatg accggcaatc
     3121 atttcttccc cttccatatc cgcaattgtt ctggctacct tcaggatcct atgataggat
     3181 ctggcactga atgcgatttt ttcaaaggct cttgccagaa gtcgggatgc gctgtctgta
     3241 agagggcaga atttatcgat cagactgctc ttcagctgcc cgttaaaatt tattttctca
     3301 tctttatacc gttcttcctg gatcttctgt actttttcca ctctttttct gatagcagcg
     3361 gaattttctc cctttcttcc acagtgaagc tgtgaaaaag aaaccggcgg tacctcagtg
     3421 ctgatatcaa ttctgtccag aagggggcga ctgatttttc ccatataatg tatgatctca
     3481 cctgctgtac aggtgcatct gtttaaatcc ggataataac cgcagggaca gggattcatc
     3541 gctgcacata acataaaatc cgccggaaag ttgtaagttc cgcttgctct ggctatctgg
     3601 atcactttat cctccatggg ctgtcgcaga agttccagac ttgctctgga aaattccggc
     3661 atttcgtcca gaaagagtac acctttatgc gcaagagtga tttcgccggg tctgggatta
     3721 cgcccgcctc ctgcaattgc ctgcggagta cttgtatgat gagggctgcg aaatggtcgc
     3781 tcatcgatca gaggatgttc tcttgaaaga agtcctgcaa tactgtaaat tctggtaagc
     3841 tccagctttt cttcaaaacc aagcccaggc atgatcgtcg gcagtcttct ggcaagcatg
     3901 gttttgcccg ttcccggagg tccgataagg agaagattgt gaaatccgct tactgcaatt
     3961 tccgccgctc tttttgcccc ttcctgtcct tcaatgtctg aaaaatccat tcccatatct
     4021 gtatttataa tagaaggaat ttcttcctgt atttccctct tcagataggg ttccggattc
     4081 ttaagacact cgaccagttc ccgtaaattt tttactcctg ctaccggcac atccctgatt
     4141 agtcttcctt ctttcagatt ctcataggga actacgcaaa aacgactgcc caaacttctt
     4201 gcacacaaca caatcggcag gattccggat atcccatgga tttctccgtc cagaccgatc
     4261 tcccctgcca tcataactct gctgacaacc tgagggctga tcatctcaaa agcagccaac
     4321 actgctgctg ccaccggtac atcaaaaccg gctccctctt tcttcatgtc tgcaggagca
     4381 aaattgacgg taattctctt tggtggaaac tgataaccat tattcttgag tgctgtgcgt
     4441 acccgttcct gcgcttcctt tacctgcgca gaaggaaatc ctaccatgat aaaggaagga
     4501 agtccgttac ttacatcagc ttctacctgc acagggcata cttccacccc aaaaattgca
     4561 gctgataata cacttgcaaa caaatataaa ctcctttctt ccggcataaa tctcccccga
     4621 tatttatgct cacaaaaaaa atatataaca attcgttact gttcacagta acgtagccaa
     4681 aattcattcc ggattgcctg cggcaatgga attttggctt gtatgtctcg cccaacagcg
     4741 gacgactgaa tagttacatc acttaaattg tattggaaat tatttcagaa atcagatttg
     4801 aaaaaagatt atttttataa tagaccattt tgctctgttt tgcaaccgtt cattttgttc
     4861 tgttttatac ttttttcagt acataatctg cgaaggcttt tcacagatca tgtttgggag
     4921 tacactacaa aacagagagg tgaactatat gaaatttcag cgtattcagg atttacgtac
     4981 agatgctgac atgtcacaga aacagctcag cgaaattctt cacatcagcc agcgttccta
     5041 ctctcactat gagacaggtt cacgcaatat cccggttgaa atgcttatca gactggcaaa
     5101 ttattacgat ataagtgttg attatctggt aggacgcact gataaaaaag agatgaataa
     5161 ataagtaaaa tcactttttt taatataaaa ggattcctgt aactgtttat gttactgaat
     5221 ccttttatat tttttgtatc ttttattctt ttatcataac agtatccaca gaaggatgga
     5281 cttttgcacc ctccgctttt gtttttgtct taatattcac ataaagacga ccattatcaa
     5341 aggtaagata accctttgct acatttccca gcgagtctgt aaacgagaat ctggttgcat
     5401 ttcctgcgac ggatttcttt acttttgcct tgcaggtggc tgtctttttc ttatttgtct
     5461 gtgaataagt aaaggtaacc gacttctgac tgagtttctt aattttgatc ttaaccctgc
     5521 cgttcttact gcgccaggtt cccagataat cctccgggtt aaaaacataa gcccgttcca
     5581 gaaccggtgt tggcgttgcc gtaggaatcg gtgttggggt aggtgtgggc gtagctgcag
     5641 gtgtcggtgt tacagtggga actctttcca gtgccgcgat cgcttctgct tttctctgct
     5701 ctctgtccac attctttctg tgttgaagca gcagagtgcc tgccagcata agcaggataa
     5761 acaggataat tacaattatc agaagtttta taagtctttt tttcttttta ttccgtttct
     5821 gtttcccgtt gtttgttacc gacaaatctg catctcctct tctgtttctt gttcgttact
     5881 tattcttttc catcccagca ataggtacag agtttacatg gtgaaattcc aatggaagct
     5941 ttcagatcat caagtctgtg gaatttcagt gatgtgaaat tcagctcctt acggatttct
     6001 tccagcattt ctttataatt tgttgaattt ggatctgcat agtctgccag gactttatca
     6061 ctgacattct ctccctcgcg tttggcgatc acacgtcttg tgatcagatc catctctgat
     6121 tttgaacgtg agaaattcaa atatttgcat ccataaagta aaggcggaca cgcaggacgg
     6181 atatgtactt cttttgcacc atttctgtaa agaaattccg tcgtttctct taactgtgtt
     6241 ccacgtacaa tagaatcatc aatcatcaga agactcttat ccttgatcag gcgatgtaca
     6301 ggaatcagct tcattcttgc gataagattt ctctgactct gctgtgtagg cataaatgat
     6361 cttggccagg ttggtgtata tttgataaac ggacgagcaa aaggcacacc ggactggttc
     6421 gcatatccga ttgcatgggc aataccggaa tccggaacac cggctacaat atcgggattt
     6481 acttctcccc tgtctctcct ggccagcata tcaccgcaat gataacgcat ttc
//
LOCUS       DBMDXG010000073         6527 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3012_length_6527_cov_1.547463, whole genome shotgun sequence.
ACCESSION   DBMDXG010000073 DBMDXG010000000
VERSION     DBMDXG010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3012_length_6527_cov_1.547463"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            269..592
                     /gene="ylxM"
                     /locus_tag="ACZPD3_03380"
     CDS             269..592
                     /gene="ylxM"
                     /locus_tag="ACZPD3_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR1415081.1"
                     /translation="MEKFVEQGYLYDFYGELLTERQQQVYESVVLEDYSLSEVAEDLG
                     ISRQGVHDMIKRCNKTLEEYEQKLHLVEKFLNIRAQVKQIRVLAREYHSDEIVNISNE
                     ILEEL"
     gene            596..1951
                     /gene="ffh"
                     /locus_tag="ACZPD3_03385"
     CDS             596..1951
                     /gene="ffh"
                     /locus_tag="ACZPD3_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR1415082.1"
                     /translation="MAFESLTDKLQNVFKKLRSKGRLTEEDVKLALKEVKMALLEADV
                     NFKVVKQFTKSVQEQAIGQDVMSGLNPGQMVIKIVNDELVKLMGSETTDLPLRPGNEI
                     TVFMMAGLQGAGKTTTVAKLAGKLKSKGKKPLLVACDVYRPAAITQLQVNGEKQGVEV
                     FSMGDKQKPVDIAKAAIEHAKANQQNVVLIDTAGRLHVDEDMMQELADIKANIQVDAT
                     ILIVDAMTGQDAVNVASTFADKVGIDGVILTKMDGDTRGGAALSIKAVTGKPILYVGM
                     GEKLSDLEQFYPERMASRILGMGDVMSLIEKAEASIDKEQAQDMQKKLKKMDFDFNDY
                     LTSMEQMNKMGGIGSILNMLPGMGGKMKDIEGMIDEKAMDRTKSIILSMTPQERSNPS
                     ILNISRKNRIARGAGVDVSEVNRLVKQFEQSKKMMKQMPGLMGGKAGKRGGGFKLPFG
                     L"
     gene            2065..2310
                     /gene="rpsP"
                     /locus_tag="ACZPD3_03390"
     CDS             2065..2310
                     /gene="rpsP"
                     /locus_tag="ACZPD3_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR1415083.1"
                     /translation="MAVKIRLRRMGQKKAPFYRIVVADSRCKRDGRCIEEIGTYDPNL
                     EPSAVTVDEEAAKKWLAAGAQPTDVVAKILKDAGIEK"
     gene            2374..2604
                     /locus_tag="ACZPD3_03395"
     CDS             2374..2604
                     /locus_tag="ACZPD3_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR1415084.1"
                     /translation="MKELVEVIAKSLVENPDEVVVTETEKEDAIIVELKVGPADMGKV
                     IGRQGRIAKAIRTVVKAASSKSSKKVIVDILQ"
     gene            2749..3252
                     /gene="rimM"
                     /locus_tag="ACZPD3_03400"
                     /pseudo
     CDS             2749..3252
                     /gene="rimM"
                     /locus_tag="ACZPD3_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704681.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
     gene            3253..4011
                     /gene="trmD"
                     /locus_tag="ACZPD3_03405"
     CDS             3253..4011
                     /gene="trmD"
                     /locus_tag="ACZPD3_03405"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR1415085.1"
                     /translation="MNFHVLTLFPEMIENGMNTSITGRAITKGLLTLEAVNIRDYAFN
                     KHQKVDDYTYGGGAGMLMQAEPVYLAYEAIANRTAKKPRVVYLTPQGQVFNQAMAREM
                     AQEEDLVFLCGHYEGIDERVLEEIVTDYVSIGDYVLTGGELPAMVMMDSISRMVPGVL
                     NNQESGETESFAGNLLEYPQYSRPEEWHGKKVPEVLMSGHHANIEKWRREQSIYRTAK
                     RRPDLLKKADLTNKEWNYVRQLRKQWKEEEEQIK"
     gene            4008..5033
                     /gene="queA"
                     /locus_tag="ACZPD3_03410"
     CDS             4008..5033
                     /gene="queA"
                     /locus_tag="ACZPD3_03410"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR1415086.1"
                     /translation="MKTSDFYYDLPQELIAQDPLEDRSSSRLMHLSLKDGSIEHRHFT
                     DVLDYMEEGDCLVINDTKVIPARLYGHKEETGALIEILLLKRRENDIWECLVKPGKKA
                     RPGAKITFGNGILKGEIIDVVDEGNRLIQFHYEGIFEEILDQLGEMPLPPYITHKLKD
                     KNRYQTVYAKNEGSAAAPTAGLHFTKELLEKVKEKGVNIAHVTLHVGLGTFRPVKVDD
                     VESHHMHSEFYIVEEDQAKLINDTKKAGKRVIAVGTTSCRTLESATGEDGILKSGSGW
                     TEIFIYPGYHFKMIDALITNFHLPESTLVMLVSALAGKENIMHAYETAVQEKYRFFSF
                     GDAMIII"
     gene            complement(5211..>6527)
                     /locus_tag="ACZPD3_03415"
     CDS             complement(5211..>6527)
                     /locus_tag="ACZPD3_03415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR1415087.1"
                     /translation="YYQEKRQKLLIATGIGIFVLVFILILAGIRGCSNYMSPRQAAAK
                     KTVSMNASKDNSQKAYSDSQNTDSSNATVSSPVSLTLSVVGDCTLGTDETFDYDTSLN
                     AYYENYGADYFLQNVKDIFSADDLTIANFEGTLTDSDEREDKTFAFKAPASYASILTG
                     GSVEAVNTANNHSHDYGDQSFDDTLAALDDAGIVHFGYDETAVMDVKGIKVGLVGIYE
                     LYDHLEREQQLKDNIAKVKADGAQLIVAIFHWGNETETVPDSNQTTLGRIAIDEGADL
                     VCGHHPHVLQGIETYKGRNIVYSLGNFCFGGNSSPSDMDTMIYQQTFTIDADGVKKDN
                     VTNIIPCSISSAAYDGYNNYQPTPAEGDEATRILGKINERSSWISTAEGSTFTAKYNS
                     NNDSQSSSADTAASDSDIVDMNSSASDDTDAETYDESYDTDNSDAE"
BASE COUNT         2047 a         1194 c         1627 g         1659 t
ORIGIN      
        1 taaaaatatg atagaatgca ggtcctacga tgtcaattcc ttttgtagat gattccggaa
       61 acatcaggag gcaggtatca gacattgtat ttgcaaattc tgttggacgt gtctggaatg
      121 aacctggtaa gacaaaaaga tggcaaatca gagaagtgtt gattttacgc aggataacac
      181 aagacaaaac aagataaaat ttagttcgtc aagagaaaat gcttgacagc acattggaaa
      241 taggttaata tagtcaaggt gattacagat ggagaagttt gtagagcaag gttatttata
      301 tgatttttat ggagaacttc tgacagagag acagcagcag gtttatgaga gcgtggtgct
      361 ggaggattac tcgctgagtg aagtcgcaga ggatctgggc atcagcagac agggtgtgca
      421 tgatatgatc aagagatgca acaagaccct tgaagagtat gaacagaagc ttcatcttgt
      481 agagaaattc ctgaatatac gggcacaggt aaagcagatc cgtgtacttg cccgggaata
      541 tcattccgat gaaattgtaa atatttccaa tgagatttta gaggagttat aacatatggc
      601 atttgaaagc ttgacagata aactgcagaa tgtatttaag aagttgcgaa gcaagggccg
      661 cctgacagag gaggacgtta agcttgccct taaggaagta aagatggctc tgttagaggc
      721 agacgtaaac tttaaggttg tgaagcagtt tactaaatct gtacaggagc aggcaatcgg
      781 acaggacgtt atgagcggtc tgaatccggg tcagatggtg atcaagattg ttaatgatga
      841 acttgtgaaa ttaatgggaa gtgagacaac agatctgcct cttcgcccgg gaaatgagat
      901 cacagtgttc atgatggcag gtctgcaggg tgcaggtaag accaccacag ttgcgaagct
      961 tgcaggtaag ctgaaatcca aaggtaagaa acctctttta gtagcctgcg atgtttaccg
     1021 cccggcggct atcacacagc tgcaggtcaa cggcgagaaa cagggcgtgg aagtattttc
     1081 catgggagat aagcagaagc ctgtggatat tgcaaaagca gccatagagc atgcgaaggc
     1141 aaatcagcag aatgtcgtac ttatcgatac agccggtcgt cttcatgttg atgaagatat
     1201 gatgcaggaa cttgcagata tcaaggcgaa cattcaggta gatgcaacca tcctcattgt
     1261 agatgcgatg accggacagg atgcggtaaa tgtggcaagc acatttgctg ataaagtagg
     1321 aatcgacggt gtcattctta ccaagatgga tggtgatacc cgaggcggtg cagccctttc
     1381 tatcaaggca gtaaccggta agcctattct gtatgtaggt atgggcgaga aactttctga
     1441 tctggaacag ttctatccgg aacgtatggc ttccagaatc cttggtatgg gtgatgtcat
     1501 gagcctgatc gagaaagcag aagcttccat cgataaagaa caggcgcagg acatgcagaa
     1561 gaagttaaag aaaatggact ttgacttcaa cgattacctt accagtatgg aacagatgaa
     1621 caagatgggc ggtatcggca gtatcctgaa tatgcttccg ggtatgggcg gtaagatgaa
     1681 ggatattgag ggaatgatcg atgagaaagc catggacaga accaagtcaa ttatcctttc
     1741 catgacacct caggagagaa gtaatccgtc aatactgaac atttccagaa agaaccgtat
     1801 tgccagaggt gcaggagtag atgtctctga agtaaaccgt ctggtgaaac agtttgagca
     1861 gagcaagaag atgatgaaac agatgcctgg tcttatgggc ggaaaagccg gtaagcgcgg
     1921 cggtggattt aaacttccgt tcggactgta attgagaagt tagcgaagcg gataacgctt
     1981 tgacataaaa gttactgttt catagctgaa cagtaatata acataagaaa acaatatatt
     2041 ttatttaatg gaggaaaaga aacaatggca gttaaaatca gattaagaag aatgggacag
     2101 aagaaagcac ctttttatag aatcgtagtt gcagattccc gttgcaaacg tgatggaaga
     2161 tgtatcgaag aaatcggaac atacgatcca aaccttgagc caagcgctgt aacagttgat
     2221 gaagaagcag ctaaaaaatg gcttgcagca ggagctcagc ctacagacgt tgtagcaaaa
     2281 atcctcaaag atgccggaat cgaaaaataa ttcagattta ccatgtaagg tatttctgca
     2341 gcgtccacag gggacgggtc agggaggtat gtaatgaaag aattagtgga agtaattgca
     2401 aaatctcttg ttgaaaatcc ggatgaagta gttgttactg agacagaaaa ggaagacgcg
     2461 attatcgttg aactgaaagt cggacctgct gacatgggca aggttatcgg acggcaggga
     2521 agaattgcga aagcgatccg aactgtggta aaagcagctt cttcaaaaag cagcaaaaaa
     2581 gtgattgtag atattctgca atgaaaataa gggtcaggag ctgtggaaca tcccatggct
     2641 cctgatttgg gtttaagcgg atttggaata tggatcagca ttcgctgtga gcaggcataa
     2701 aagcagaatg caaatgacgt atgatatgaa aattatagga gtcagcagat ggaagattta
     2761 ttaaaagttg gtgtgatcac tacgacacac ggaatacgcg gggaagtaaa agtatatcct
     2821 accacagatg cagacagatt tctggatctg gaatatgttc tgcttgacac aggcagagag
     2881 aaaagaaagc ttgaaataga gaatgttaag tatttcaaga accttgtaat cctgaaattc
     2941 aggggaattg ataatattaa tgatatagag atgtataaaa aaagagaact ctggatcccg
     3001 agagaagaag cgcaggagct ggaagaagat gaatattaca ttgcagacct gatcggtatg
     3061 gatgtagttc tcgaagactg aagcaaattt ggaactctga aagatgtaat ggaaacaggc
     3121 gctaatgatg tctatgtagt agaggatgac aaaggcgaag aaattctgct ccctgctatc
     3181 agggaatgta tccttgatgt ggatgtggag aagaatgtaa tgaccattca tctgatgaaa
     3241 ggattaatat aaatgaattt tcatgtttta accctgtttc cggagatgat tgaaaatggc
     3301 atgaatacca gcattaccgg acgtgcgatc acaaaaggtc tcctcacact ggaggcagtg
     3361 aacattcgtg attatgcatt caataagcac cagaaagtgg atgattatac ctatggcggc
     3421 ggtgccggaa tgctgatgca ggcagaaccg gtttaccttg cttatgaggc gattgcaaac
     3481 cgtacggcaa aaaagccaag agttgtatat ctgactcctc aggggcaggt atttaaccag
     3541 gctatggcca gagaaatggc gcaggaagag gatctggtat tcctttgtgg acactatgag
     3601 ggaattgatg agcgtgttct ggaggaaatc gtcacagatt acgtatctat cggagattac
     3661 gtgcttacag gcggcgaact tcctgcaatg gtcatgatgg attccatttc aagaatggtt
     3721 ccgggtgtac tgaacaatca ggagtccggt gagacagaat cctttgcagg aaaccttctg
     3781 gagtatccgc agtacagccg tccggaagag tggcatggca aaaaagtacc ggaggttctt
     3841 atgtccggtc atcacgcaaa catagaaaag tggcgcagag agcagtcaat ttaccgcacg
     3901 gcaaaaagac gcccggatct tcttaagaaa gctgatctga caaacaaaga atggaactat
     3961 gtgcgccagt taaggaaaca gtggaaggaa gaagaggagc agataaaatg aaaacatcag
     4021 atttttatta tgaccttcct caggaactga tcgctcagga tcctctggag gacagaagtt
     4081 catccagact gatgcatctg tctttaaaag atggcagcat tgagcatcgc catttcacag
     4141 atgttctgga ttatatggaa gagggagact gcctggtcat caacgataca aaggtaatcc
     4201 ctgcccggtt atacggacat aaagaagaga caggagcact aatcgaaatc ctgcttctga
     4261 agcgaagaga gaatgatatc tgggagtgtc tggtaaaacc cggaaagaag gcacgcccgg
     4321 gtgctaagat cacatttgga aatggtattt taaaaggtga gattattgat gtggtggacg
     4381 agggaaaccg tctgatccag ttccattatg aaggaatctt tgaggaaatc ctggatcagc
     4441 ttggcgaaat gcctctgccg ccttatatta cacataaact gaaagataag aaccgttatc
     4501 agacagttta tgcaaagaac gaaggctccg cagcagcacc gacagcagga ttgcacttta
     4561 ctaaagagct tcttgaaaaa gtaaaggaaa aaggcgtcaa tatcgctcat gtgactcttc
     4621 atgtaggtct cggaactttc cgccctgtca aagtagatga cgtggaaagc caccatatgc
     4681 attccgaatt ctacatagta gaggaggatc aggcaaagct gatcaatgac acaaagaagg
     4741 caggtaaacg tgtaatagca gtaggaacca caagctgcag aacactggaa tctgcaacag
     4801 gagaagatgg aattctgaaa tccggaagcg gatggacaga aatctttatc tatccgggct
     4861 atcatttcaa aatgatcgat gccctgatca caaacttcca cttgccggaa tccacacttg
     4921 tgatgttggt gtctgcgctg gcaggcaagg agaatatcat gcatgcctat gagactgcag
     4981 tgcaggaaaa gtacagattt ttctcttttg gagatgctat gattatcata taatgtcacc
     5041 caaataatta tatcaatcaa ttatctggtt atgagcaagt agcgaagagg atgcttttat
     5101 ctgcttgata aaagcatagt aaaaatataa aaaagagctg agaagtcctt aactggattt
     5161 ttcagctctt ttgaagtatg gatgtgattt tggatgaatg aaggctctat ttattcggcg
     5221 tccgaattat cagtatcata ggattcgtcg tatgtctctg cgtctgtgtc atctgaagct
     5281 gaactgttca tatcaacgat atcgctatct gaagcagctg tatctgcaga actgctctgg
     5341 gagtcattat tactgttata ttttgcagta aaagtactgc cttctgcagt agaaatccag
     5401 gaactccgct cattgatttt tccaaggatt cttgttgcct catcaccttc tgccggagtt
     5461 ggctgataat tattatatcc atcataggca gcagaagaaa tggagcaggg aatgatattg
     5521 gtcacgttgt ctttttttac cccatcggca tcaatagtaa aggtctgctg gtagatcatg
     5581 gtgtccatat cacttggaga actgttgccg ccgaagcaga agtttccgag gctgtataca
     5641 atattcctgc ctttataagt ttcaatgccc tgaagtacat gcggatgatg tccgcataca
     5701 agatcagcgc cttcatcgat cgcgatgcgt ccaagtgttg tctggttgct gtccggaacg
     5761 gtttccgttt cattacccca gtggaagatc gctacgatca gctgtgcccc gtctgccttt
     5821 acttttgcaa tattgtcttt taactgctgc tcgcgttcaa gatggtcgta cagttcatag
     5881 ataccgacta atcctacttt gattcccttt acatccataa cagcagtttc atcatatccg
     5941 aaatgaacga ttcctgcatc atccagtgct gccagagtat catcgaaact ctgatcaccg
     6001 taatcatgac tgtgattatt agcggtgttt acagcttcta cagacccgcc tgtcagaata
     6061 gaagcatagg atgcaggtgc tttaaacgca aatgttttgt cctcccgttc atcagaatcg
     6121 gtgagtgttc cctcaaaatt cgcaatagtc agatcatctg cggaaaaaat atcttttaca
     6181 ttctgcagga aataatctgc accgtaattt tcataataag catttaagct ggtatcataa
     6241 tcaaaagttt catctgtgcc aagagtgcag tcaccgacaa cgctgagtgt cagagaaaca
     6301 ggagaagata ctgtagcatt ggaggaatcc gtgttctgag aatctgagta tgctttctgg
     6361 ctgttgtcct tagaagcgtt catggaaaca gtttttttgg ctgctgcctg tctggggctc
     6421 atatagttgc tgcatccgcg gatacctgcc agaataagta taaaaaccag aacaaagata
     6481 ccgatacctg ttgcaatcag caatttttgc cttttttcct gatagta
//
LOCUS       DBMDXG010000074         6480 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3043_length_6480_cov_1.806710, whole genome shotgun sequence.
ACCESSION   DBMDXG010000074 DBMDXG010000000
VERSION     DBMDXG010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3043_length_6480_cov_1.806710"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="ACZPD3_03420"
     CDS             <1..396
                     /locus_tag="ACZPD3_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR1415088.1"
                     /translation="ILALLFVGAGASGTADYFAGFIGNKLSIPLGQMFFRAVLCNFFV
                     CLGVLCGMKLKSDAGRFLMIVMCISGFVVSGFEHCIANMGIFVTAYCLVPGLSLGAMV
                     KSMVVVTLGNMVGGALLLAWPLRKMSADK"
     gene            398..2578
                     /locus_tag="ACZPD3_03425"
     CDS             398..2578
                     /locus_tag="ACZPD3_03425"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1415089.1"
                     /translation="MSKALYIAEKPSVAQEFAKALKINGQRRDGYLESQDSVVTWCVG
                     HLVTMSYPEKYDIKYKRWSLDTLPFLPREFKYEVIPGVQKQFEIVKGLLNREDVDTIY
                     VCTDSGREGEYIYRLVAQMAGVHGKKEKRVWIDSQTEEEIMRGIREAKDLSAYDNLSA
                     SAYLRAKEDYLMGINFSRVLTLRYGNSVSNYLNTKYQAISVGRVMTCVLGMVVRRERE
                     IRAFVKTPFYRVLSCIALEGENFEGEWRAVEGSRYFQTPYLYKENGFKEKAYAEKLIQ
                     ELSVSQPLQCTVEKIERKKENRNPPLLFNLAELQNVCSKLFKISPDETLKIVQELYEK
                     KLVTYPRTDARVLSTAAAKEIYKNISGLRNYEHTAEIAQHIIEQGNYKNLAKTRYVND
                     KQITDHYAIVPTGQGLNTLRSVSLTAQRVYETIVRRFVCIFYPPAVYQKISLVTKIQN
                     ESFFSSFKVLLDEGYLKIATNSFAKRKAADAMSSVNRAGAADSEGSEEEDPDTGKNGG
                     NKADDSAEDMACDTRLLAALQNLKKGDILSVDSLSIKEGETSPPKRYNSGSMILAMEN
                     AGQLIEDEELRAQIKSCGIGTSATRAEILKKLCNIKYLALNKKTQVITPTLLGEMIFD
                     VVNCSIRQLLNPELTASWEKGLNYVAEGSITEQEYMDKLEHFVRLRTRQVEDSNIQPY
                     LRQFFDAAAVNYKDSSKKNSAKTTGRSTSAAGRSRTCRKPSASK"
     gene            2641..3312
                     /locus_tag="ACZPD3_03430"
     CDS             2641..3312
                     /locus_tag="ACZPD3_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR1415090.1"
                     /translation="MLKDFTIANLLDLNETIAKDLFEGKTYPWEVLPEIGDFILKLGQ
                     TLSEEEYDHPSEDVWIAKSAKVAPTACINGPVIIGKEAEVRHCAFIRGKAIIGEGAVV
                     GNSTELKNAVLFNKVQVPHYNYVGDAVLGYKSHMGAGSICSNVKSDKKLVVVKDGDEK
                     IETGLKKFGAMLGDNVEVGCGSVLNPGTVIGRCCNIYPLSSVRGCVPANHIYKSKTEI
                     AEKRS"
     gene            complement(3420..4160)
                     /locus_tag="ACZPD3_03435"
     CDS             complement(3420..4160)
                     /locus_tag="ACZPD3_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR1415091.1"
                     /translation="MTTKLPASFLPAKFQVLSGSALKLIAITLMLIDHTGLMILYNYP
                     ATTATLFSFGGVDYSWYRIFRDIGRAAFPIFCFLLVEGFLHTHNRKKYGLNLFLFACI
                     SEIPWNFMFTNTWRYEKQNVFFTLFLGYLAFCALEYFWDNQKMQLVCVLALLTVSVFL
                     KADYGWRGFVFLLIMYWFRNDKTAQAIIGSCWLYYEWKACFAFLSINMYNGERGFVKG
                     RFLKYVFYWFYPVHITILVILRKLLFHM"
     gene            4413..5105
                     /locus_tag="ACZPD3_03440"
     CDS             4413..5105
                     /locus_tag="ACZPD3_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR1415092.1"
                     /translation="MIYSREVEEMCPVAQGVHHGAAPIPEEAKWVQAKEVKDISGLTH
                     GVGWCAPQQGACKLTLNVKEGIIQEALVETIGCSGMTHSAAMASEILPGLTVMEALNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSEEGLPVGAGLEDLGKGLRSQVGTMYGTLKK
                     GPRYLEMAEGYVTGIALDADDEIIGYQFVSLGKMTDFIKKGDDPNTAWEKAKGQYGRV
                     ADAVKIIDPRKE"
     gene            5124..6134
                     /locus_tag="ACZPD3_03445"
     CDS             5124..6134
                     /locus_tag="ACZPD3_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HIR1415093.1"
                     /translation="MALFESYERRIDKINSVLNSYGIASIEEAEKITKDAGLNVYDQI
                     KGIQPICFENACWAYTVGAAIAIKKGCKKAADAAAAIGEGLQAFCIPGSVADTRKVGL
                     GHGNLGKMLLEEETECFCFLAGHESFAAAEGAIGIAEKANKVRQKPLRVILNGLGKDA
                     AQIISRINGFTYVETEYDPYKNEVKEVFRKAYSEGLRAKVNCYGANSVPEGVAIMWKE
                     DVDVSITGNSTNPTRFQHPVAGTYKKERLEAGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTLGRMHSDAQFAGSSSVPAHVEMMGLIGAGNNPMVGMTVAVAVSIEEAAKAG
                     KF"
BASE COUNT         2060 a         1245 c         1566 g         1609 t
ORIGIN      
        1 attttagcat tgttgtttgt aggtgcagga gcatccggaa cagcagacta ttttgcggga
       61 ttcattggaa ataaactgag tattccgctc ggacagatgt tcttcagagc agtgctctgt
      121 aacttcttcg tctgtcttgg cgtgctttgt ggtatgaaat taaagagtga tgcaggaaga
      181 ttcctgatga tcgttatgtg tatctcagga ttcgttgtaa gcggatttga gcactgcatc
      241 gcaaatatgg gaatctttgt taccgcatac tgcctggtac ctggattatc ccttggagcc
      301 atggtaaaga gcatggtagt agtaacttta ggaaatatgg tgggcggtgc cctgttgctg
      361 gcatggccgc tcagaaagat gagtgcagat aaataatatg tcaaaagctt tatacatagc
      421 cgaaaagccc agtgtggcgc aggaattcgc aaaagctttg aaaatcaacg gacaaagacg
      481 ggatggctac ctggaatctc aggacagcgt ggtgacctgg tgtgtgggac acctggtcac
      541 catgagctac ccggaaaaat atgatatcaa atataaaagg tggagtttag ataccctgcc
      601 ttttttgccg cgcgaattta aatatgaagt gatccccgga gtgcagaagc agtttgagat
      661 tgtcaaagga ctgcttaacc gtgaggatgt ggacaccatt tacgtgtgta ccgactccgg
      721 acgcgaggga gaatacatct accgtctggt ggcacagatg gcaggtgttc atggaaaaaa
      781 agaaaagaga gtctggattg actctcagac agaagaagag atcatgagag gaatcagaga
      841 ggcaaaggat ctgtcagcat atgacaatct ttctgcttca gcctatcttc gagcaaaaga
      901 agactatctc atgggaatta atttttcccg tgtactgaca ctgcgttatg gaaacagtgt
      961 gtcaaattat ttaaatacga aatatcaggc catttctgta ggacgggtta tgacctgtgt
     1021 gcttggaatg gtagtgcgaa gagaacgcga gatccgtgcg tttgtgaaaa ctccgtttta
     1081 ccgcgtactc agctgcattg cccttgaggg agaaaacttt gagggagaat ggagagctgt
     1141 ggaaggcagc agatatttcc agactcctta tctgtataaa gagaacggat ttaaagagaa
     1201 agcctatgct gaaaaactga tacaggagct gtctgtgagt cagccactgc aatgtacagt
     1261 agagaaaatc gagcggaaaa aagagaacag gaatccgccg cttttattta acctggcgga
     1321 gcttcagaat gtgtgctcca agctgttcaa gatcagcccg gatgagacat taaaaatcgt
     1381 acaggaactg tatgaaaaga aactggtcac ttatcccaga acagatgccc gtgtattgtc
     1441 tactgcggca gcaaaggaga tttataagaa tatttccgga ctgcgtaatt atgaacatac
     1501 agcggaaatc gcacagcaca ttattgaaca gggaaattac aaaaatctgg caaagaccag
     1561 atatgttaat gataagcaga tcacagacca ttatgcaatc gtacccacag gccagggctt
     1621 aaacacattg agaagtgttt ctcttactgc gcagcgtgta tatgagacca ttgtcagaag
     1681 gtttgtatgt attttttatc ctcctgcagt atatcagaag atcagtcttg tgacaaaaat
     1741 tcagaatgag agcttctttt cttcatttaa agtactgctg gatgaagggt atttaaaaat
     1801 tgccacaaat tcttttgcga agcgaaaagc agcagatgcc atgagcagtg tgaacagagc
     1861 cggtgcagcg gacagtgagg gaagtgaaga ggaagatccg gatacaggaa aaaacggcgg
     1921 gaacaaagct gatgattcgg ctgaggatat ggcatgtgat accagacttc ttgccgcatt
     1981 gcagaacctg aaaaaaggtg atatactctc tgtggacagc ctgagtatca aagagggcga
     2041 gacatcgccg ccaaagcgtt ataattccgg ttctatgatt cttgcaatgg agaatgcagg
     2101 tcagctgatc gaggacgaag agcttcgtgc ccagattaaa agctgcggaa tcggaaccag
     2161 tgccaccaga gcggaaattc ttaagaaact gtgcaatatc aaatatctgg cactcaataa
     2221 gaaaacccag gtgatcactc caacccttct tggagagatg atctttgatg tggtaaactg
     2281 ctccatcaga cagcttctga acccggaact gactgcaagc tgggaaaaag gactgaatta
     2341 tgtggcagag ggcagtatta cagaacagga atatatggat aagctggaac attttgtgcg
     2401 ccttcgcacc agacaggtgg aggacagcaa tatccagcca tatctgagac agttctttga
     2461 tgctgcagca gtgaattata aggattcatc taaaaaaaac agtgcgaaaa caacaggcag
     2521 atcaacgtcc gcagcgggac ggtcacgtac ctgcagaaag ccgtctgctt caaagtagtg
     2581 tgtatttgtg acagataaaa gaaaacatgt catcatagcg agagaaaaga ggaaaatatt
     2641 atgttaaaag attttacaat tgcaaacctt ctggatctga acgaaacaat cgcaaaggat
     2701 ttatttgaag gaaaaacata tccatgggaa gtattaccgg agatcggaga tttcattctg
     2761 aaactgggac agaccttaag tgaagaagaa tacgaccacc cgtcagaaga tgtatggatt
     2821 gctaagagtg ccaaagtggc acctacagcc tgtatcaacg gtccggtcat cataggtaaa
     2881 gaagcagaag tacgccactg tgcatttata cgcggaaaag caattatagg tgagggtgca
     2941 gtagtaggta attctacgga attaaagaat gcggtacttt tcaacaaggt acaggttccg
     3001 cactacaatt atgtaggcga tgcagttctc ggctacaaat cccatatggg tgcaggttcc
     3061 atctgttcca atgtaaaatc tgacaagaaa ctggtagttg taaaagacgg tgacgagaag
     3121 attgaaacag gtctgaagaa attcggcgca atgcttggag ataacgtaga agtgggatgc
     3181 ggttccgtat taaatcccgg aactgtgatc ggacgctgct gcaacattta tccactttcc
     3241 tcagtaagag gctgcgtacc tgcaaatcac atttataagt caaaaacaga aattgcagaa
     3301 aagagaagtt gaaaatatag aagaggcagg ttgtatgaag gatattattt caatcataca
     3361 gcctgccctt ttatattttt atgtctgtct gagatagaag atatttgttg tacctgatat
     3421 tacatatgaa ataaaagttt tctcaggatt accagaatgg taatgtgcac cggataaaac
     3481 cagtaaaaaa catatttcag aaatcttcct tttacaaatc cacgttcacc attgtacata
     3541 ttaatggaaa gaaaagcaaa gcaggctttc cattcatagt aaagccagca gcttccgatg
     3601 atcgcctggg cggttttatc gttgcggaac cagtacatga tcagcaggaa tacaaatccc
     3661 cgccatccgt aatctgcctt cagaaacaca gagacagtca gcagtgccag cacgcagaca
     3721 agctgcattt tctgattatc ccagaagtat tccagggcac agaatgccag gtatcccaga
     3781 aacagggtga aaaatacatt ctgtttttca tatctccagg tgttggtaaa catgaagttc
     3841 catggaatct cagagataca tgcaaataga aacagattca ggccatattt cttacggtta
     3901 tgtgtatgca gaaatccttc cactaacaga aaacagaaaa ttggaaaagc ggccctgccg
     3961 atatcccgga aaatccgata ccagctgtaa tccacgccac cgaaactgaa aagggttgca
     4021 gttgtggcag gataattata caggatcatt aagccggtat ggtcaatcag catcagggtg
     4081 atagcaatta attttaaagc gcttccgctc agaacctgaa attttgcagg gagaaaggaa
     4141 gcaggtaact ttgttgtcat aatagaaatc ccttcattaa tagaaattag aactatttta
     4201 ccacaaaaaa atcaaagatg gttaaaaatt ttacaattta tgactggacg aatcagcact
     4261 tttgtgagaa aataacacta ggttgttagg catactcctc tgaagtcatt cagcagacat
     4321 cggagtttta tcctgtgcag ttcattcagc agttctgtac agtggagagt ataacaacat
     4381 gaagttaatc tatacttgaa aggagtttta caatgattta ttcacgcgaa gtagaagaaa
     4441 tgtgcccagt tgcacagggc gttcatcatg gcgccgctcc aatcccagaa gaagctaaat
     4501 gggtacaggc aaaagaagtc aaagacattt ccggtcttac acacggtgtt ggctggtgtg
     4561 caccacagca gggtgcctgc aagcttacat taaatgttaa agaaggtatc atccaggaag
     4621 cactggttga gacaatcgga tgttcaggta tgactcactc tgcagctatg gcttccgaga
     4681 ttcttccggg acttacagtt atggaggcac tgaacactga cttagtttgt gatgcgatta
     4741 atacagctat gagagaactc ttccttcaga tcgtttatgg acgtacacag tctgcattct
     4801 cagaagaagg acttccagta ggtgctggac ttgaagacct tggtaaagga cttcgttctc
     4861 aggttggtac aatgtatgga actctgaaaa aaggacctcg ttaccttgaa atggcagaag
     4921 gttatgtaac aggtattgct cttgatgcag atgatgagat catcggatat cagttcgtaa
     4981 gccttggaaa aatgactgat ttcatcaaaa aaggcgatga cccgaatact gcatgggaaa
     5041 aagcaaaagg tcagtatggc cgtgttgcag acgctgttaa aattatcgac ccaagaaaag
     5101 aataatttag gaggtaacag gaaatggctt tatttgaatc atatgagaga cgaattgaca
     5161 agatcaattc tgttttaaac agctatggca ttgcttctat cgaagaagct gagaaaatca
     5221 caaaagacgc aggtcttaat gtatacgatc agatcaaagg cattcagcca atctgtttcg
     5281 agaacgcttg ctgggcatat acagtaggtg ctgcaatcgc aatcaaaaaa ggatgcaaga
     5341 aagcagcaga tgcagcagca gctatcggtg aaggacttca ggcattctgt attcctggat
     5401 ccgttgctga tacacgtaaa gtaggtcttg gacatggtaa ccttggcaag atgcttcttg
     5461 aagaagaaac agagtgcttc tgcttcctgg ctggacatga gtccttcgca gcagctgaag
     5521 gtgctatcgg tatcgctgag aaagctaaca aagttcgtca gaaaccatta cgtgttatcc
     5581 tgaacggtct tggaaaagat gctgctcaga tcatctcccg tatcaacggc tttacatatg
     5641 tagagacaga atacgatcca tacaagaacg aagttaaaga agtattcaga aaagcatact
     5701 ccgaaggact tcgtgcaaaa gttaactgct acggcgctaa cagcgttccg gaaggtgttg
     5761 ctatcatgtg gaaagaagac gttgacgttt ctatcacagg taactccaca aacccgacac
     5821 gtttccagca tccggttgca ggtacataca agaaagaacg tcttgaagca ggtaagaaat
     5881 acttctcagt tgcttccggt ggtggtacag gacgtacact tcacccggac aacatggcag
     5941 caggtccagc ttcctacggt atgacagata cattaggacg tatgcactct gatgcacagt
     6001 tcgctggttc ttcttccgta ccggctcacg tagagatgat gggtctcatc ggcgctggta
     6061 acaacccgat ggttggtatg acagttgcag tagctgtttc cattgaggaa gctgctaagg
     6121 ctggaaagtt ctaatcaaac ctttttaatc aaagagtcaa caaacctaga aagtaccgta
     6181 gttccaaggg ctgcggtact tttcttagtt tagatcaaaa aatgagaaag taatttgaga
     6241 cacggtgaga agccttgaaa atggcaagat aggcaaacag gtaggtaaca agtaggcaac
     6301 agagcgaagg aaacaaaaag aagagccgat cacaggaaaa gaaatgccgc aaccacaagg
     6361 gttacggcat tttttctagt gtgccgagca tggcactaat cttactggtg aaagtccagt
     6421 cacgggtatt taccgccaag tgtagtgaac cacaagtcgg taccagtaat ggtatggatg
//
LOCUS       DBMDXG010000075         6469 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3051_length_6469_cov_1.398869, whole genome shotgun sequence.
ACCESSION   DBMDXG010000075 DBMDXG010000000
VERSION     DBMDXG010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_6469_cov_1.398869"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="ACZPD3_03450"
     CDS             <1..843
                     /locus_tag="ACZPD3_03450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1415094.1"
                     /translation="KKAKEYNVLGIKISKDNELAAEWYSEPECRRNIYSATKSFTSCA
                     VGFAVQEGLIDLNEKLTEAFSGDLPECIDENLKEATVRDLLTMCLGQEKGSLMGEQRP
                     LYEEDNWVKMSLAIPFKYKPGTHFVYNNVGPYLAGILVQRRSGCDLVSYLTPRLFSKL
                     GIKRPTWETDPLGNSFGAGGLFLTLSELHKFGLFYLNKGKWNGKQILSEKWIEESTKA
                     ADVGYYGYLFWRGEYNSFRADGKYSQISMILPKKNAVVSFVSECRRGDELLKTVYELV
                     CAKL"
     gene            1073..1966
                     /locus_tag="ACZPD3_03455"
     CDS             1073..1966
                     /locus_tag="ACZPD3_03455"
                     /inference="COORDINATES: protein motif:HMM:NF023568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1415095.1"
                     /translation="MKRICIAISSNDKDTTMHLEIYAPEYKGDLKGLIQICHGMTEYM
                     GRYVEFAKYFTSRGYIVFGNDIISHGHSTTPRSSCLYINDWNDTVKDMVSAREYVVRK
                     YPNLPIYLLGFSLGSFIVRTNADLTPYKKEILIGTGAQSAFLMRIMRTWIGKKYTGKM
                     SCASDKIYDLMFGTYGKKFKGRPANYWLLTDNEKRREYADDSLVRQDVSPAFFCEFSK
                     GMECASRNLKNPNNTIPTLFLYGKKDPVSGFGKGVRKVYKAYKENNPDTEIRSFPGTH
                     DILHDSGYESVFKAIADYLRK"
     gene            complement(2225..2518)
                     /locus_tag="ACZPD3_03460"
     CDS             complement(2225..2518)
                     /locus_tag="ACZPD3_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415096.1"
                     /translation="MYATKYYYEIDKWTVVDTAKSSGNDQNPSWPEPKLKDGQRTGAE
                     EEHYFVTATYEKKKGKTETGRYEMDFSQWKELKKGEKIELKIDAAGFAEINQK"
     gene            complement(2545..3006)
                     /locus_tag="ACZPD3_03465"
     CDS             complement(2545..3006)
                     /locus_tag="ACZPD3_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR1415097.1"
                     /translation="MGKIIEGLWDCPFCGNKRIRAGQKTCPDCGHPQDENTKFYMPDE
                     IKYVSEEEAEKISRNPDWQCSFCGSLNSDDLNVCKNCGATKEDSERNYFEMRQQEEEK
                     KRKKEEKKESCQKNIPQNTPKKKPLLRRVLLILGIFAAIIFGMMSCLAPKM"
     gene            complement(3110..4117)
                     /locus_tag="ACZPD3_03470"
     CDS             complement(3110..4117)
                     /locus_tag="ACZPD3_03470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1415098.1"
                     /translation="MTEFNFDTVFDETMQTHNLDTVVILGHLNPDGDAAGSVMSLAHY
                     IHVNYPQYRVFPYLAKTLERGPKKMVVEDKIFVPFEMPDISGKQYCVIVCDNATLERM
                     IGLEYYQNAAASIVVDHHASNEGYGDVNWTKVSEACAENVYHMLSSELLLNAAQKEAY
                     PNAADYIYLGILHDTGGLARANQGIFQAVADLMAMGVDHGRIMKTLHSDTLDILYKRA
                     DILHGAVRAMDGRVAYVIMGQKEIAEKDITYEDIHCISTILRDCDDIELGFTMYEEEE
                     NGWRCSFRSDGKWINVNELLKPFGGGGHISAAGLKYQTDNVEELKKQILDRVAEMKKK
                     Q"
     gene            complement(4662..>6469)
                     /gene="feoB"
                     /locus_tag="ACZPD3_03475"
     CDS             complement(4662..>6469)
                     /gene="feoB"
                     /locus_tag="ACZPD3_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR1415099.1"
                     /translation="TSEEIVSRKFFLEDKPRGIINIVDATNIERNLYLTLQLIELDIP
                     MVLALNMMDEVRENGGTIHINELEEALGIPVIPISAARNEGIDELIDHAVHVAKYQER
                     PGRLDFCDAEGEDHGAVHRCLHSIMHLIEDHAAQADIPTRFAATKAIEGDPLILEKLK
                     LDTNETEMLEHIVQQMETERQVDRSAAIADMRFDFIERLYEQTVVKPKESKERIRSEK
                     IDKILTGKYTAIPCFIGIMVLVFYLTFNVIGAWLQGLLEIGIDKVSALVEAALTAANV
                     NSAMHSLVIDGIFTGVGSVLSFLPIIVTLFFFLSLMEDSGYVARVAFVMDKLLRKIGL
                     SGRSIVPMLIGFGCTVPAVMATRTLTSERDRKMTILLTPFMSCTAKLPIYSFFVSAFF
                     PKKGGFIMAGLYLLGILVGILAAFLYNRTLFKGDPVPFVMELPNYRLPGARNVGQLLW
                     EKAKDFLQRAFSIILLASMIIWFLQSFDLHLNLVKDSADSILAMVAGVLAPIFKPIGL
                     GDWRICTALISGFMAKESVVSTLEVLFSGNIASVLTPLAAASLLVFSLLYTPCVAAIA
                     SIKREMGSKWAVGVVVWQCVIAWVAALIVHLIGMI"
BASE COUNT         1845 a         1494 c         1264 g         1866 t
ORIGIN      
        1 aagaaagcaa aagaatataa tgttcttgga atcaaaattt caaaagataa tgaacttgca
       61 gcagagtggt acagcgaacc ggaatgcaga aggaatattt attctgcaac aaagagtttc
      121 acatcctgtg cagttggctt tgctgttcag gaagggctga tcgatcttaa tgaaaaacta
      181 acagaagcat tttcaggtga cttgccggag tgtatagatg agaacctgaa agaagcaact
      241 gttcgtgatc ttcttaccat gtgccttgga caggagaagg gcagtctgat gggagaacaa
      301 cgacctttat atgaagaaga taactgggta aaaatgtctc tggctattcc ctttaagtat
      361 aagccgggta cgcattttgt gtataataac gttggtccct atctggcagg cattcttgta
      421 cagagacgct ccggctgcga tcttgtttct taccttacac ccagactttt ttccaaactt
      481 ggaataaaac gtcctacatg ggagactgat ccacttggaa acagttttgg agccggcgga
      541 ttatttctga ccctttctga acttcacaaa tttggactgt tttatcttaa taaaggcaag
      601 tggaatggaa aacagatact gtctgagaaa tggatagaag aaagtacaaa ggcagctgat
      661 gttggatact atggttatct tttctggcgt ggtgaatata attctttccg ggcagatgga
      721 aaatactccc agatttctat gatcctgccg aaaaaaaacg cagtcgtttc gtttgtttca
      781 gaatgccgca ggggagacga acttctgaag acagtttatg agcttgtctg cgcaaaactg
      841 taatatcttt tctccgcgga aaaccagact cctcaacatc ctaaacaact tttttccagg
      901 tatgcaaaaa aacactttcc ttttctgaaa aaaagcaata taatgcttca cgcaagaaga
      961 tggttctgcc ggcgaaataa atccgaccag aagaaaaggt tatcgctgac accatctatt
     1021 ctgttttgca aaatctggca tattacagag aaataagaaa tgaggaaata tagtgaaaag
     1081 aatatgtata gcaatttcat caaacgataa agacaccacc atgcacctgg agatctatgc
     1141 accggaatat aagggcgatc tcaaaggcct gatccagatc tgccatggaa tgacagaata
     1201 tatgggaaga tatgtggaat ttgcaaagta ttttaccagc cgcggataca ttgtctttgg
     1261 caatgacatc atcagccacg gacacagcac cacaccaaga tcatcctgtt tgtacattaa
     1321 tgactggaac gacacagtta aagatatggt atcagcaagg gaatatgttg tccggaaata
     1381 cccaaacctg cctatctatc ttttgggatt ttctctgggt tccttcattg taaggacaaa
     1441 cgctgatctg actccctata aaaaagaaat cctgatcggt accggtgcgc agtccgcttt
     1501 cctgatgagg ataatgagaa cctggatcgg gaaaaaatat accggaaaga tgtcctgtgc
     1561 ttctgataag atctatgatc tgatgttcgg tacatatggc aaaaagttca aaggcagacc
     1621 ggccaattac tggctcctga cggataatga aaagagaagg gagtacgctg atgacagtct
     1681 tgttcgtcag gatgtgtctc ctgctttttt ctgcgagttt tccaaaggca tggaatgtgc
     1741 cagccgaaac ctaaaaaatc cgaataatac cattccaact ttattcctgt atggaaaaaa
     1801 agatccggta tccggttttg gaaaaggcgt ccgaaaggta tataaagcat acaaagaaaa
     1861 caacccggat acggagatca gaagctttcc cggaactcac gatatcctgc atgactccgg
     1921 atatgaatcg gtatttaagg caatcgccga ttatttgaga aaataatact acaatcagaa
     1981 cccgatgctc taccctgaaa agctgctcca gtcgtgccgg gctgtccagt cgcccttcgt
     2041 actcctcttt attctccgca tgtataataa acggagcaat atctgcctgc aggcattgca
     2101 caaatgcaat gatgcgggct accttataac atgagaaaag cattaaacaa ggagactcac
     2161 gccaaaacta tgaatctcct tgtttaataa aggttcgaag tatccctccg ggtatatcta
     2221 tatcttattt ttgattaatc tctgcgaatc cagcagcatc aatttttaat tctatttttt
     2281 cgcctttttt aagttctttc cattgagaaa aatccatttc atatcttcct gtttcggtct
     2341 tgcccttctt tttctcatat gtagcggtca caaaataatg ctcttcttct gctcctgttc
     2401 tctgtccgtc tttaagtttt ggttccggcc aggaagggtt ctgatcattg ccggaacttt
     2461 ttgcagtatc gacaaccgtc catttgtcta tttcatagta atattttgta gcatacacag
     2521 gctccttccg gtagactggt ttttctacat cttcggtgct aggcatgaca tcatgccaaa
     2581 aataattgct gcgaagattc ccagaatgag cagcacccgc ctcagcagtg gctttttctt
     2641 tggtgtattt tgggggatat ttttctggca ggattctttt ttctcttctt tctttcgttt
     2701 cttttcctct tcctgctgcc tcatctcgaa gtagtttctc tccgaatctt cttttgtggc
     2761 accacagttt ttgcagacat tcagatcgtc gctgttaaga ctgccgcaaa agctgcactg
     2821 ccagtccgga ttacggctga tcttttctgc ttcctcctca gaaacatatt taatttcatc
     2881 cggcatatag aattttgtat tttcgtcctg cggatgccca caatcgggac aggttttttg
     2941 accggctcta atccttttat tcccacaaaa cgggcaatcc cagagacctt cgattatctt
     3001 tcccatacag actctcctct tccttccctc aataacaatt tcaacaagta tttagatttc
     3061 tcttcaattt tataattttc tgcagttttt ttatttgctc atttatagat tactgtttct
     3121 ttttcatctc tgctactctg tccagaatct gtttctttaa ttcttctaca ttatcggtct
     3181 gatattttaa tcctgctgcg gagatatgtc ctccaccgcc aaaaggtttc aacagttcat
     3241 taacattgat ccatttcccg tctgaacgga aagaacatct ccacccgttt tcttcttcct
     3301 catacatggt aaaaccaagt tcaatatcgt cgcaatcgcg caggatggtg ctgatacagt
     3361 ggatgtcttc gtatgtgata tctttttcgg caatttcctt ctgtcccata ataacatagg
     3421 caacccggcc atccatcgca cgtactgctc catgaagaat atcggctctt ttgtacagaa
     3481 tgtccagggt atcactgtgt aaggttttca taatcctgcc atggtcaact cccatagcca
     3541 tcagatcagc tactgcctga aaaataccct ggtttgccct tgccagtcct ccggtatcat
     3601 gcaggatgcc aaggtaaata taatctgcgg cattggggta cgcttctttt tgggctgcat
     3661 ttagaagcag ttcggaagaa agcatatggt atacattttc cgcacacgcc tcagaaacct
     3721 ttgtccagtt cacatctccg tatccttcat tggaagcatg atgatcaaca acaatgctgg
     3781 cagcggcatt ttggtaatat tccaatccga tcatacgctc cagagttgca ttatcacaga
     3841 ctatgacaca atattgtttt ccggatatgt ccggcatctc aaaaggaaca aatatcttat
     3901 cttccacgac catctttttc ggaccgcgct ccagcgtttt tgcaagatat ggaaacaccc
     3961 tgtattgtgg ataattgaca tggatatagt gcgcaaggct catcaccgat ccggcagcat
     4021 cgccatccgg atttaagtgt cccagaatca ctacagtgtc cagattgtgc gtctgcatgg
     4081 tttcatcaaa aacagtatca aaattaaact ctgtcattcc tatctccttc gtatatcact
     4141 tccctgttat attttaattt tatcatctaa cagatcagat agcaagttgt gggcacatac
     4201 aagccaaaat tcgttactgt tcactccgtt cacagtaacg tggccaaaat tcattacaga
     4261 ttgcattatt ttgcggaatt gctatagaac caaaagagac aaaagagacc acttgtctct
     4321 ttttaaaaaa ctatagcaat ttcgtaagat ttctatagtt ttttcagggt ttctgtacta
     4381 taatgaaacc cataaagaga ttgtcatatc aaaaatagta atctaatttt ctgctagaaa
     4441 atcagatcac ctcccatcaa actggaaatt cagggaaagt tgttctgata ttattatgaa
     4501 aaagatgatc gaagcccagg aaagacagat gtccagtggt tcccagggcg gacagtacta
     4561 atcagataaa agcctctcag ttgaggaact gaaaaaaacc gatggtgatg actgaaaaca
     4621 gtcaaatcca tcggtttttc tataaaagcg aattaataaa atcagatcat ccctatcaga
     4681 tgtacgatca gtgcagccac ccaggcaatc acacactgcc ataccacaac accaacagcc
     4741 catttactgc ccatttcacg tttgatcgaa gcgatagccg ccacacatgg tgtatagagt
     4801 aaactgaata ccagcaggga tgctgctgcc agcggagtca gtacagaggc aatatttcca
     4861 ctgaaaagta cttccagagt agataccacg ctctcttttg ccataaatcc gctgataaga
     4921 gctgtacaga ttctccagtc tcccagtccg atcggtttaa atataggtgc caggactccc
     4981 gccaccattg ccagaatact gtctgcagaa tcttttacaa gatttaaatg cagatcaaag
     5041 ctctgcagga accagattat cattgatgca agcaggataa tagaaaacgc tctctgcagg
     5101 aaatcttttg ctttttccca caaaagctga cctacattcc ttgctcccgg cagccggtaa
     5161 ttgggaagtt ccatgacaaa cggtacggga tctcccttga aaagagttct gttatacaaa
     5221 aatgccgcca ggattcccac caaaattcca agcaggtaaa gacctgccat aataaatccg
     5281 cccttcttcg ggaaaaaagc acttacaaag aaagaataaa tcggcagctt cgcagtacag
     5341 ctcataaatg gagtcagcaa aatcgtcatc tttctgtcac gctcacttgt aagtgtacgt
     5401 gtggccataa cagccggaac cgtacatccg aatccaatca gcattggcac aatgcttctt
     5461 cctgaaaggc cgatcttacg cagaagtttg tccatcacaa aagcaacacg tgcaacgtat
     5521 ccgctgtctt ccataagaga caggaagaaa aacagggtta cgataatcgg cagaaaactg
     5581 agtacacttc ccacacctgt aaaaataccg tcaatgacca ggctatgcat ggcactgttt
     5641 acatttgctg ctgtcagtgc tgcttctacc agcgcagaaa ccttatcaat tccaatctcc
     5701 agaagcccct gaagccacgc tccgatcaca ttaaaggtaa gataaaatac caataccatg
     5761 attccgatga aacagggaat cgctgtatat tttcctgtaa gaatcttatc aattttctca
     5821 cttctgattc tttctttgct ttctttaggc tttacaactg tctgttcata caaacgctca
     5881 ataaagtcaa aacgcatatc tgcgatcgca gcgcttctgt caacctgacg ctctgtttcc
     5941 atctgctgta cgatatgctc cagcatctcc gtctcatttg tgtccagctt cagcttctca
     6001 agaatcagcg gatctccctc gattgccttg gttgctgcaa aacgggtagg aatatcagcc
     6061 tgcgccgcat gatcctcaat caggtgcatg atagagtgga ggcaccggtg aacagcgccg
     6121 tgatcctctc cttcggcatc gcagaaatcc agccttccgg gacgttcctg atacttcgcc
     6181 acgtgaacag cgtgatcgat cagctcatcg ataccctcgt ttctcgcggc ggagatcgga
     6241 ataaccggta tacctagggc ttcctccagt tcgttgatgt gaatggttcc gccgttttct
     6301 ctgacttcgt ccatcatgtt cagagccagg accataggaa tatccagttc aatcagctgc
     6361 agtgtcagat agagatttcg ctcaatgttg gtagcgtcga caatattgat tatacctctt
     6421 ggtttatcct caagaaagaa ttttctcgat acgatctctt cgctggtat
//
LOCUS       DBMDXG010000076         6420 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3075_length_6420_cov_1.996360, whole genome shotgun sequence.
ACCESSION   DBMDXG010000076 DBMDXG010000000
VERSION     DBMDXG010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3075_length_6420_cov_1.996360"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="ACZPD3_03480"
     CDS             complement(<1..1127)
                     /locus_tag="ACZPD3_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1415100.1"
                     /translation="MARLNECILYRNFEHGEILDKMAELMNAWEQKAPDLKEKEGLFF
                     ECANGLVETAGAYGFSGNLWHCYLTFLLVNNENAFSTACEIRGAVNGSINELALNDFG
                     VFKELYDFDLTVLDEAFGISCCKVLGDYTNTGSNSKMFNSRIRDRICDLSKTLAAAES
                     TEEFMKDMVQFYKDFGVGKLGLHKAFRVGHDENDNVEIQPITRIAHVKLEDLVGYEIP
                     KQKLIENTEAFVRGRKANNCLLFGDAGTGKSSSIKGILNRYYDEGLRIIEVYKHQFQD
                     LNDVIAQIKNRNYKFIIYMDDLSFEEFEIEYKYLKAVIEGGLEKKPDNILIYATSNRR
                     HLVREKSSDKLEIMDDDDLHSSDTVQEKLSLVYRFGVRIYYG"
     gene            1225..1293
                     /locus_tag="ACZPD3_03485"
                     /pseudo
     CDS             1225..1293
                     /locus_tag="ACZPD3_03485"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            complement(1313..2443)
                     /gene="pheA"
                     /locus_tag="ACZPD3_03490"
     CDS             complement(1313..2443)
                     /gene="pheA"
                     /locus_tag="ACZPD3_03490"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR1415101.1"
                     /translation="MIDLQECRNEIDKIDSDIIRLFEQRMKVCEDVAEYKIRTGKKVL
                     DPERERQKLEVLRGKAHGEFNQLGAQELFQQIMAISRKRQYQLLTEHGIEDDEKLEMV
                     DALPLKDVRVVFQGVEGAYSYAAMREYFQDDIESFHVKTWRDAMEAVVEGRADYAVLP
                     IENTTAGIVADIYDLLTEYELSIVGEQIIRPEHVLLGLPDAELEDIRQVCSHPQALSQ
                     CGKYLESHPDWKKKEMENTAGSAKKIKEDNDKTQAAIASRQAGELYGLKILAENICYN
                     GQNATRFVIVSKKPIYVKDAHKISIFFELHHESGTLYNMLSHIIYNGLNMTKIESRPI
                     TGKNWQYRFFVDFEGNLKDSAVKNALRGIEAEADRMRILGNY"
     gene            complement(2465..3748)
                     /locus_tag="ACZPD3_03495"
     CDS             complement(2465..3748)
                     /locus_tag="ACZPD3_03495"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR1415102.1"
                     /translation="MNYQESREYIDKITREIPSVLGLEHMRELMKRLGNPQDDLKYVH
                     VAGTNGKGSVIAFLYSALSGARYRIGRYVSPTLYSYRGRMEVSGSRISREDFAAYITQ
                     VSDVIAAMTKDGYPHPTAFEIETAVAFLFFKKENCDLVLLEVGMGGNLDATNIIKNTL
                     LAVLVSISMDHMSFLGNTLAQIAEKKAGIIKDGCRVVTARQKPEAMQVIERISREHGT
                     KCTIADASEAEVLKESCLGQTIRYRGEEYEIPLAGVYQKENAVVALNALKVLDELGFP
                     TAAEQKKEGLRTVNWNGRFTVICKKPLFVVDGAHNPAAADMMAASIEHYFKGKRIIYI
                     MGVFADKDYRSVIQKTAHFADRILTIQTPDNIRALPAGELAKTVSEYNPNVQAMDTIK
                     DAVEEAFSLAGEQDVIIAFGSLSFIGEMTDIVENL"
     gene            3914..4300
                     /locus_tag="ACZPD3_03500"
     CDS             3914..4300
                     /locus_tag="ACZPD3_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1415103.1"
                     /translation="MKKAFILCGLTGWCMEILWTGLHSILSGELTMTGKTSLLMFPIY
                     GCGAIIRPLSGKLSAVPLFVRGCIYTVGFFFVEFISGALLRCFHMCPWDYSNTPLNYR
                     GLIRPDYAPLWFGAGLFFEKILGKLS"
     gene            4379..5302
                     /gene="pyrB"
                     /locus_tag="ACZPD3_03505"
     CDS             4379..5302
                     /gene="pyrB"
                     /locus_tag="ACZPD3_03505"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR1415104.1"
                     /translation="MRHLMSPLDLSVDELNTLLDLASDIEKHPDKYAHVCDDKRLATL
                     FYEPSTRTRLSFEAAMMNLGGKVLGFSSADSSSASKGESVADTIRVVSCYADICAMRH
                     PKEGAPLVASMASSIPVINAGDGGHQHPTQTLTDLLTIRSLKGRLDNLTIGLCGDLKF
                     GRTVHSLIEALVRYTNVKFILISPEELRIPSYIREDVLKKNNIEFQEVERLEDALPDL
                     DILYMTRVQKERFFNEEDYVRMKDFYILDKQKMELAKKDMYILHPLPRVNEISTEVDA
                     DPRAAYFKQAQYGVYVRMALILTLLEVKLPC"
     gene            5296..5733
                     /locus_tag="ACZPD3_03510"
     CDS             5296..5733
                     /locus_tag="ACZPD3_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HIR1415105.1"
                     /translation="MLNVGALREGYVLDHIKAGKAMTIYHDLKLDKLDCTVAIIKNAR
                     SNKMGKKDIIKVECPVENLDLDILGFIDHNITVNVIKDEKIVAKKELKLPNQVVNVIK
                     CKNPRCITSIEQGLDQIFVLADKEKEVYRCKYCEEKYTGKRNK"
     gene            complement(5785..6135)
                     /gene="rnpB"
                     /locus_tag="ACZPD3_03515"
     ncRNA           complement(5785..6135)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPD3_03515"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1700 a         1613 c         1199 g         1908 t
ORIGIN      
        1 ccatagtaaa tacggacacc aaaacgataa accagtgaaa gcttctcctg cactgtatcg
       61 gaggaatgca gatcgtcatc atccatgatc tccagcttat cactggattt ctctcttacc
      121 agatgtcttc tgttacttgt tgcatagatc aggatattgt ccggcttctt ctccagaccc
      181 ccctcgatca ctgccttcag atatttatat tcgatttcaa attcctcgaa tgacagatca
      241 tccatataga taatgaattt atagtttctg ttcttgatct gggcgatcac atcattcaga
      301 tcctgaaact gatgcttata cacctcgata atacgaagtc cctcatcata ataacggttc
      361 aggattccct tgatacttga ggatttacct gtacccgcat caccaaaaag cagacagttg
      421 tttgcctttc tgcctcttac gaaagcttct gtattctcga tcagtttctg cttcgggatt
      481 tcatatccga ccaggtcttc cagtttcaca tgagcgatcc ttgtgatcgg ctggatctcc
      541 acattatcat tctcatcatg gcctacacga aaagccttat gtaatcccag cttacccaca
      601 ccaaaatcct tataaaactg taccatatcc ttcatgaatt cctctgtgga ctccgctgct
      661 gccagcgttt tgctcagatc acagatacgg tctctgatac gggaattaaa catcttgctg
      721 ttacttccgg tgttggtata atcaccgaga accttacagc aggaaattcc aaatgcctca
      781 tccagtacag tcaggtcaaa atcatacagt tctttaaaca caccgaaatc attcagtgcc
      841 agttcattaa tacttccgtt gacagcccct ctgatctcac aggctgtact gaatgcattt
      901 tcattattta ccagaagaaa tgtcagataa cagtgccata aatttccgga aaatccataa
      961 gcgccggcag tttccaccag tccgttggca cactcaaaga acagcccttc cttttctttc
     1021 agatcaggtg ctttctgttc ccaggcattc atcagttctg ccattttatc aaggatttct
     1081 ccatgttcaa agttacggta taagatgcat tcatttaatc tagccatttt tattacttcc
     1141 ttcttacctt gttgttttat cttattgttc tgctgtgagt ggtataatta tttcgaaaac
     1201 gatgtaccag agtgtgtttg aaaaatgctt tacgcaaaat atgccgcaaa gtgggacgtg
     1261 cagattgtgg aaatgatttt tcaaacacgc tctggcatta tggaaatgat ttttaataat
     1321 ttcccaaaat tctcattctg tcagcttctg cctcaattcc tctcagggca ttcttcactg
     1381 cactgtcctt taaatttccc tcgaaatcaa caaaaaaacg gtactgccag ttcttccctg
     1441 tgatcgggcg ggattcaatc ttcgtcatat tcagtccatt ataaataatg tgagacaaca
     1501 tattgtaaag agttccgctc tcatgatgca gttcaaagaa aatactgatc ttatgtgcat
     1561 ccttcacata gatcggtttc ttactcacga tcacaaaacg tgtggcattc tgtccgttat
     1621 agcagatatt ctcagccagg atcttcagtc cgtaaagctc tcctgcctgt ctgctggcaa
     1681 tagctgcctg cgtcttgtca ttgtcttctt ttatcttctt ggcagaaccg gcagtatttt
     1741 ccatctcttt cttcttccag tccggatgag attccagata ttttccacac tgggaaagtg
     1801 cctgaggatg agaacatacc tgtctgatat cctccagttc agcatccggc agtcccagaa
     1861 gtacatgctc cggacggatg atctgttctc ctacaatgct aagctcatac tctgtaagaa
     1921 gatcataaat atcagcaaca ataccggcag ttgtattctc aatgggaagt accgcatagt
     1981 ctgctcttcc ctccacaact gcttccattg catcgcgcca ggtttttaca tgaaaactct
     2041 caatatcgtc ctggaaatat tccctcattg cagcatagct gtatgcacct tccacaccct
     2101 gaaagacaac tcttacatct ttcagcggaa gtgcatctac catttccagt ttctcatcgt
     2161 cctcgattcc atgctctgtg agaagctgat actgtctctt acggctgatc gccatgatct
     2221 gctgaaaaag ctcctgcgca ccaagctgat taaattctcc atgcgcctta cctctcagga
     2281 cctccagctt ctgtctctct ctttccggat ccagaacctt ctttcctgta cgtatcttat
     2341 attctgcaac atcctcacac accttcattc tctgttcaaa aagacgtatg atatcgctgt
     2401 cgatcttatc tatttcattt ctgcattcct gtaaatctat catttatgtt ataccttttt
     2461 actttcacaa attctctaca atatcagtca tctctccgat aaaggaaaga gaaccaaagg
     2521 caatgatcac atcctgttcc ccggcaagtg aaaaagcctc ctccactgca tctttaatcg
     2581 tatccattgc ctgtacattg ggattgtatt cactgacagt cttcgccagc tctccggcag
     2641 gcaacgccct tatattatcc ggagtctgga tcgtaagtat ccggtctgca aagtgcgcag
     2701 ttttctgaat cacacttctg taatccttat ctgcaaacac tcccataata tagatgatcc
     2761 gttttccctt aaaataatgc tctattgaag ctgccatcat atccgcagca gcaggattat
     2821 gagccccgtc taccacaaac agcggttttt tacagataac tgtaaaccgt ccgttccagt
     2881 ttactgtgcg aagcccttct tttttctgct ccgcagcagt gggaaagccc agctcatcca
     2941 atacttttaa agcattcagc gcaactactg cattttcctt ctgataaaca ccagccagcg
     3001 gaatctcata ctcttcaccc ctgtaccgga ttgtctgtcc aaggcagctt tcctttagca
     3061 cctctgcctc agaagcatct gctatggtac attttgtacc atgttctctg ctgatcctct
     3121 cgatcacctg cattgcttcc ggcttctgtc tggcagtcac cactctgcag ccgtctttga
     3181 tgatcccggc tttcttctct gcgatctgtg ccagagtatt tccaagaaaa gacatatgat
     3241 ccatactgat cgataccagc actgccagca gcgtattctt aataatattt gtggcgtcca
     3301 gatttccgcc cattcccact tccagcaaaa cgagatcaca attctctttt ttaaagaaca
     3361 gaaatgccac cgcagtctct atttcaaatg cagtgggatg gggataacca tcctttgtca
     3421 tagcagcaat aacatcagac acctgtgtaa tataagcagc aaagtcctct ctgctgatcc
     3481 tgcttcccga aacttccatc ctgcctctgt aagaatacag ggtaggcgaa acatatcttc
     3541 caatgcgata tctcgcacct gataaagcag aatacaaaaa agcaatgaca gaacccttgc
     3601 cattggtacc ggctacatgc acatatttca gatcatcctg tggatttcca agacgtttca
     3661 tcagctcacg catatgctcc agtcccagga cactgggaat ttcccgggtg attttgtcta
     3721 tgtactcccg gctttcctga taattcataa taaactcctc ttatttccat gagatcccct
     3781 ccacacgttc cttttgcgca taaggcacct cattgctata gtgtaataac gtgttctcat
     3841 tatatcctac gtgtatgttt ttttcaaggc aggcaatact agaatcatag aaatttatac
     3901 aggagcgtta cctatgaaaa aagctttcat tctctgcggt ctgacgggct ggtgcatgga
     3961 aatcctctgg accggactgc attccatcct ttcaggagaa cttaccatga caggaaaaac
     4021 ttccctttta atgtttccca tttacggatg cggtgccatc atccgcccgc tctccggtaa
     4081 actttctgct gtcccgcttt ttgtccgcgg ctgcatttat actgtcggat ttttcttcgt
     4141 ggaatttatc agcggtgctc ttttgcgctg ttttcatatg tgtccctggg actacagcaa
     4201 cactcctctg aattaccggg gactgataag accggactac gctcccctct ggttcggagc
     4261 cggacttttt tttgagaaaa tacttggaaa actctcttga aaaaagcacc tgattattat
     4321 atgatgttat gaaggattaa ataaaaattc ctgaaccagt aaaatatgga ggtatgaaat
     4381 gagacactta atgagtccgc tggatctgtc tgttgatgag ttaaatactc ttcttgacct
     4441 ggccagcgat attgagaaac accctgataa atacgcacat gtctgcgatg ataaaagact
     4501 ggcaacttta ttctatgagc caagcacccg tacccgtctc agctttgaag ctgctatgat
     4561 gaaccttggc ggtaaagttc ttggtttttc atcagctgat tccagttccg cttcaaaggg
     4621 tgaaagtgtt gccgatacaa tccgcgttgt atcctgttac gctgacatct gtgctatgcg
     4681 ccacccgaaa gaaggtgctc cgttagtagc ttctatggct tcttctattc ctgttatcaa
     4741 cgccggcgac ggtggtcatc aacatcctac ccagacatta accgacttac ttaccatccg
     4801 ttccctgaaa ggacgtctgg acaatctgac cattggtctg tgcggcgacc tgaaattcgg
     4861 acgtacagtt cactccctga tcgaagcact tgtacgttac acaaatgtaa aattcattct
     4921 gatctcaccg gaagagcttc gcatcccaag ctatatccgc gaggatgttc tgaaaaagaa
     4981 caatatcgaa tttcaggaag tagagagact ggaagatgcc ttaccggatc ttgatatcct
     5041 ctacatgaca cgtgtacaga aagaacgttt cttcaacgaa gaagactatg tccgcatgaa
     5101 ggatttctac attctggaca aacagaaaat ggaacttgca aaaaaagaca tgtacattct
     5161 tcatccgctt ccacgtgtaa atgaaatctc aacagaggtg gatgcagacc cgagagcagc
     5221 ttatttcaaa caggcacagt acggtgtata tgtacgtatg gccctgattc tcacattact
     5281 ggaggtaaaa ttaccatgtt aaatgttgga gcacttcgcg aaggttatgt attagaccat
     5341 atcaaagcag gcaaggccat gactatctat catgatctga agcttgataa acttgactgc
     5401 accgttgcca tcatcaagaa cgcgcgcagc aacaaaatgg gtaagaaaga tatcatcaag
     5461 gttgaatgcc cggttgagaa tcttgatctt gacattctcg gctttatcga tcataacatt
     5521 actgtaaacg taatcaagga cgagaagatc gttgccaaaa aagaactgaa acttccgaat
     5581 caggttgtca acgtgatcaa atgcaagaat ccacgctgca tcacttccat tgaacaggga
     5641 ctcgaccaga tctttgttct tgcagataaa gagaaagaag tttacagatg taaatactgc
     5701 gaagagaaat atacaggtaa acgcaataaa taataaaaca aatatcaaaa aaacagaacg
     5761 gaagaaatcc gttctgtttt tttgtacgca atcagaccga taagccgggt tatgtcattg
     5821 ggtaatcatc tgtctaggcc ttacgttgcc ataaggctca agcgacccac ctgaaagcac
     5881 gacgggcagc cgtatcgctt tctattcggt cttgcttcga atggagttta catgtgcccg
     5941 gcctgttacc aggacggcgg tagtctctta cactgccttt ccacccttac ctcagcaagt
     6001 gaggcggttt atttctgttg cactggtctg ggagtcacct ccaccggacg ttatccggca
     6061 tcctgccctg cgaagcccgg actttcctcg tctgaaccct ttcgtctgtt cagccgcgat
     6121 tacctggcct ggttgcgcaa gtgctattct agcacgtttt tttgcacttg taaagaaatt
     6181 tcattcatta ctgttcactc cgttctcagt aacgttgcca aaattcattt caaattgcct
     6241 gcggcaatgg aattttggct tgtatgtctc gggattttgg catattcatg ccaaaacacc
     6301 tcgcgggata gtggcgtgtg aacagtaact ttcattctag tacatcgttt tcgaaataac
     6361 tataccactc acttgtctgc gaatccgatt gcacaggtct aaaagcctca gcgtagcccg
//
LOCUS       DBMDXG010000077         6399 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3088_length_6399_cov_1.886154, whole genome shotgun sequence.
ACCESSION   DBMDXG010000077 DBMDXG010000000
VERSION     DBMDXG010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3088_length_6399_cov_1.886154"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="ACZPD3_03520"
     CDS             <1..818
                     /locus_tag="ACZPD3_03520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1415106.1"
                     /translation="HKVPGKIVPLIAIPTTAGTGSEVTAFSVITDHSRDYKLTVFSYE
                     LLPAYAILDPELLTSAPASVAAACGIDAFIHAEEAYVSTAASPFSDAMAEKAMELIGG
                     NIRRFVARRTDLEAAEAMLTGSLFAGIAFSFARLGNVHAMSHPVSAFFNVAHGVANAV
                     LLPVIAEYNALADHGRYLKIYNYISPIPAYEDEFEPLMLVDAIRELNEDIGIPEDLTT
                     AIRQAKKVEEISDEEIESRIDAMADDAMKSGNIAVNPRSSRKQDIVKLYYKAL"
     gene            1088..1744
                     /locus_tag="ACZPD3_03525"
     CDS             1088..1744
                     /locus_tag="ACZPD3_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H1 domain-containing protein"
                     /protein_id="HIR1415107.1"
                     /translation="MAKKKIYAVRKGHKTGLFATWAECQKAVSGYSGAEFRGFTEKEE
                     ALAFLNMETTGNVSRDKAKEAAGIVEVPENMVIAYVDGSFEKSIGRYAFGCVMLTPDG
                     QEIRKSGSGSDSAGVAIRNVAGEMLGSMTAVNWAIENKYPAVEIRYDYEGVEKWVTGV
                     WRAKTPLTSKYAAHMQEAGKKVKISFCKIAAHTGNHYNEEADQLAKSALLKMDEEVRI
                     "
     gene            complement(1934..3025)
                     /locus_tag="ACZPD3_03530"
     CDS             complement(1934..3025)
                     /locus_tag="ACZPD3_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HIR1415108.1"
                     /translation="MEKFKAFLRRKDIEISIKRYGIDALGAMAQGLFCSLLIGTIINT
                     LGTQFHISFLTTAVATVNDTQYTVGSLASAMSGPAMAVAIGYALHCPPLVLFSLITVG
                     FASNALGGAGGPLAVLFVAIFASEIGKAVSKETKIDILVTPLVTIGVGVALSAWWAPA
                     LGSAAMKVGNIIMWATNLQPFLMGILVSVFVGIALTLPISSAAICAALGLTGLAGGAA
                     VAGCCAQMVGFAVMSFRENKWGGLVSQGIGTSMLQMGNIVKNPRIWIAPILTSAITGP
                     LATCLFKLQMNGTPVSSGMGTCGFVGQIGVYTGWMNDIAAGTKTAVTGWDWAGLLLIS
                     FILPAILCPLINMFIRKLGWVKDGDMTLS"
     gene            3371..4006
                     /locus_tag="ACZPD3_03535"
     CDS             3371..4006
                     /locus_tag="ACZPD3_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczE/YyaS/YitT family protein"
                     /protein_id="HIR1415109.1"
                     /translation="MSEWIRKINWKYVVVMLIGNIILGLGIAIFKLSGLGNDPFSGMV
                     MALAECVGIEYARFLILLNLGFFVIEIIWGRKLIGLGTIINALFLGYFVTFFYNLITS
                     VIDAPDQMAMQVVTVFIGVIITSLGISMYQLPKQGVAPYDSISLIMTEKWPKIPYFWC
                     RVSNDAISALVCWLAGGIVGLGTLVSAFGFGPFVQFFDTHFTSKVLAKLEK"
     gene            complement(4086..4955)
                     /gene="sdaAA"
                     /locus_tag="ACZPD3_03540"
     CDS             complement(4086..4955)
                     /gene="sdaAA"
                     /locus_tag="ACZPD3_03540"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR1415110.1"
                     /translation="MDFKNANELLALCEEKNLPISEIMRIREIELGETASEIVNKKMT
                     RVLGIMKDAAFSPIRQPVKSMGGLIGGEARKLSLHAQEKKGLCGNLLQKAITYAMATL
                     ETNASMGLIVASPTAGSAGIVPGLMLAMQEHYQFSDEEIIRALFNASAIGYLAMRNAT
                     VAGAVGGCQAEVGVASAMAASAAVELMGGSPKECTFAASTVLMNMLGLVCDPVGGLVE
                     YPCQNRNAAGVANAIIAAEMALAGIPQLIPLDEMIQTMFTVGKKLPAELRETAMGGCA
                     TTPSACEACHICS"
     gene            complement(4975..5643)
                     /gene="sdaAB"
                     /locus_tag="ACZPD3_03545"
     CDS             complement(4975..5643)
                     /gene="sdaAB"
                     /locus_tag="ACZPD3_03545"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIR1415111.1"
                     /translation="MSFISVFDVMGPNMIGPSSSHTAGAARISYLAQKMIEGPLKRAD
                     FILYGSFAKTYHGHGTDRALLGGIMGFSTDDMRIRNSFDIAHEKGLKFSFTPNEQETD
                     IHPNTVDICMENEKGQKMTVRGESLGGGKVRIVDINHVQVDFTGEYSAVIVIHQDTPG
                     VVAYITKCLSDRNINIAFMRLFRESKGEIAYTIVESDGKLPENIVPAIRENSNIHEVM
                     IVQM"
BASE COUNT         1908 a         1262 c         1459 g         1770 t
ORIGIN      
        1 cacataaggt tcccggaaaa attgttccgc tgatcgcaat cccgactact gcgggaaccg
       61 gttcagaggt tacggcattt tctgttatca ctgaccacag cagagattat aagctgacgg
      121 tattcagtta tgaacttctt cctgcatatg cgattctgga tcctgaactt ctcacatctg
      181 ctccggcatc tgtggcagca gcctgcggaa tcgatgcatt tattcatgcg gaggaggctt
      241 atgtttccac agcagcatct cctttttctg acgcaatggc agagaaagca atggagctga
      301 tcggtggaaa tatccgccgt tttgtggcaa gacgtacaga tctggaggct gcagaggcaa
      361 tgcttacggg atctcttttt gcaggaatcg ccttttcctt tgcaagactt ggaaatgtcc
      421 atgctatgag tcatccggta agtgctttct ttaatgtggc acatggagtg gcaaatgcag
      481 ttcttctccc tgtgattgca gaatataatg cgctggcaga tcatggcaga tatctgaaga
      541 tttataatta tatcagtccg atccctgcat atgaggatga attcgaacca ctgatgctgg
      601 tggatgccat ccgggaatta aacgaagata tcggaatccc ggaagacctg accacagcca
      661 tccgccaggc aaagaaggta gaagagattt ctgatgagga gattgaaagc aggattgatg
      721 ccatggcaga tgacgccatg aagagcggaa atattgcagt aaatccaaga tcctccagga
      781 aacaggatat tgtgaaactg tattataaag ctttataaga taaagaataa tgcagtattt
      841 tatgtgattg ctacaggtag gatattgatt aaaaatatct gttcttataa aaaacgtgca
      901 gaaacatcga agtcgacaga taacttcagg cttgaaggtt tatctcgatt tcaaaatgta
      961 tctgcacgtt atttttttga gaaatttcca ttgtataact tgcctgatag aaagaaagat
     1021 tatatagtat taaccaaaat atagtaaagt aatgtgagtc aaaatattga ttgttcagga
     1081 ggattttatg gcaaagaaga aaatatatgc agtacgcaag ggacataaaa caggactttt
     1141 tgctacatgg gcggagtgcc agaaggcagt aagcggatat tcaggagctg agtttcgggg
     1201 atttacagag aaagaggaag cattggcttt tctgaacatg gagacaacag gaaatgtatc
     1261 ccgtgataaa gcaaaagagg cggcaggaat cgtggaagta cctgaaaata tggtcattgc
     1321 ttatgtagat ggaagtttcg agaaaagtat tggcagatat gcctttggtt gtgtgatgct
     1381 tacaccggac ggacaggaaa tccgtaaatc cggcagcggt agtgattctg caggagtcgc
     1441 gatccgaaat gtggcaggag agatgcttgg ttccatgact gcagtaaact gggccataga
     1501 aaacaaatat ccagctgtgg aaatccgcta tgattacgaa ggggtagaga aatgggtaac
     1561 aggtgtatgg agagccaaaa cacctctgac cagcaaatat gctgctcata tgcaggaagc
     1621 aggaaagaaa gtaaagattt ctttttgtaa aatagcagct catacgggca atcactacaa
     1681 tgaagaggca gatcagctgg ctaagagtgc attgcttaaa atggatgaag aagtacgaat
     1741 ataaaaatct tatcactgaa aggcgatttc caaagggatg aaaaatttat gtggatgaca
     1801 atttgagtga tcctcaggga aatcaatata cggaagtgga aaaataaaaa gcagctgtgt
     1861 taccattgaa actgttagct gaaattttta tcaagtaaca gtttcaattg acaggtaacg
     1921 cagctgtttt tgcttatgaa agtgtcatat ccccgtcttt cacccatcca agcttacgga
     1981 taaacatgtt gatgagcgga cacaggattg caggcaggat aaaggaaatc agcaaaagtc
     2041 cggcccagtc ccagcctgtg acagctgttt ttgttcctgc ggcaatatca ttcatccatc
     2101 cggtgtagac accaatctgt cctacgaaac cgcaggtacc cataccggaa gaaaccggag
     2161 taccgttcat ctgaagtttg aacagacagg ttgcaagagg tccggtgatg gcagaagtaa
     2221 ggatcggtgc aatccagatg cgtggatttt ttacgatatt tcccatctgg agcatggatg
     2281 ttccgattcc ctgagaaaca agtccgcccc atttgttctc acggaaggac attactgcga
     2341 aacctaccat ctgtgcgcag catcctgcaa cagcagctcc tccggcaagt ccggtaaggc
     2401 caagcgcagc acagatggcg gcagatgaga taggaagtgt cagtgcaata cccacgaata
     2461 cagaaacaag gattcccatc aggaacggtt gcaggttggt tgcccacata ataatatttc
     2521 ccactttcat tgctgcactt ccaagtgccg gagcccacca tgcggacagg gcaacaccta
     2581 caccgatcgt gacaagtgga gtgaccagaa tatcaatttt ggtttcctta gatactgctt
     2641 ttccgatttc ggaagcgaag atggcaacaa ataatacagc cagtggtccg cctgcaccgc
     2701 ctaatgcgtt agatgcaaag ccaacagtga tcagtgagaa caacaccaac ggcggacaat
     2761 gcagcgcata gccgatggcg acagccatgg caggaccgct catggcagat gccagagagc
     2821 ctacggtata ctgtgtgtcg tttacagttg ccacagcagt ggtcagaaat gaaatatgaa
     2881 actgtgttcc aagtgtgttg atgatcgtgc caatcagcag ggagcagaaa agaccctgcg
     2941 ccatggcacc aagggcatcg attccataac gttttatgga gatttcgatg tctttacgtc
     3001 tgagaaatgc tttaaacttt tccatagatt actttctcct gtttttatta tgtatagaag
     3061 ggtgtatata cacctatata cgtttttttc attatagtca cattcacgga gtaaatcaag
     3121 tagttttgta actgttcaca caccactatc atgcgaggtg ttttggcatg aatatgccaa
     3181 aatcacaaga catacaagcc caaattccat taccgcaggc aatctggaat gaattttggg
     3241 tacgttactg tgaacggagt gaacagtaac gtagttttaa aatagtggaa aacaatttac
     3301 agaaagaata ttgtaaagta ttttactgtc tgatatgata gaaagagtta attttacgga
     3361 ggaaaaaaag atgagtgaat ggatcagaaa aataaactgg aagtatgtgg tcgtaatgtt
     3421 gattggaaat ataatccttg gtttgggaat tgcgatattt aaactttccg gacttggaaa
     3481 tgaccctttc agcggaatgg taatggcact ggcagaatgt gtgggaattg aatatgcccg
     3541 ttttttaatt cttctgaatc tgggattttt tgtaatagaa attatctggg gaagaaagct
     3601 tatcggattg ggaacgatca taaatgcgtt attccttgga tattttgtta catttttcta
     3661 taacctgatc acatctgtga tcgatgcacc ggatcagatg gcaatgcagg tggttaccgt
     3721 atttattgga gttatcataa caagccttgg aatttccatg taccagcttc cgaaacaggg
     3781 agttgcacca tatgacagta tttctctgat catgacagag aaatggccga aaattccgta
     3841 tttctggtgc agagtatcta atgatgcgat cagtgcactg gtgtgttggc ttgcaggggg
     3901 aatcgtcgga ctgggaacac tggtcagtgc atttggattc ggaccttttg ttcagttttt
     3961 cgatacacat tttacatcaa aagtgctggc aaagctggaa aaataataaa aaactgtcct
     4021 gtatatgcac attatacgta gaaaataggt gcattacaga gacagttttt ttatttgtca
     4081 gcctgttaac tgcatatatg gcatgcttca catgcagatg gcgtagtagc gcatcctccc
     4141 atagcagttt cacgcagttc tgcaggaagc tttttaccca cggtaaacat cgtctggatc
     4201 atttcgtcca gaggaatcaa ctgcgggatt cctgcgagag ccatttctgc ggcaattata
     4261 gcgtttgcca cacctgcagc atttctgttc tgacatggat attctaccag accgccgacc
     4321 ggatcgcata ccagaccaag catgttcata agaacagtag atgcagcaaa agtacattct
     4381 ttcggtgaac ctcccatcag ttctacagca gcagatgcag ccattgcaga tgcaacccct
     4441 acctcggcct ggcatccgcc taccgcacct gcaacagttg catttcgcat tgccagataa
     4501 ccaatggcac ttgcgttaaa taatgcccgt ataatctctt catcagagaa ttgataatgt
     4561 tcctgcattg ccagcatcag ccccggtacg attccggcag aaccggcagt aggagaggcc
     4621 acgatcagcc ccatggaggc attcgtttcc agtgtagcca tagcataagt aattgccttc
     4681 tgaagcagat ttccgcagag accttttttc tcctgagcgt ggagactcag ctttctggct
     4741 tcgccgccga tcagaccacc catagatttt accggctgtc tgattggtga gaaagcagca
     4801 tctttcataa ttcccagtac tcgtgtcatt tttttattta caatctcaga ggctgtttcg
     4861 ccaagctcaa tctcacggat acgcataatc tctgagatag gaagattttt ttcttcacag
     4921 agtgctaaga gttcatttgc atttttaaaa tccatgataa aaatcctcct ttctttacat
     4981 ctgtacgatc ataacttcat gaatattcga gttctcgcga atcgcaggaa cgatattctc
     5041 aggaagtttg ccgtcagatt ctacgattgt ataagcaatc tctcccttgc tttcacggaa
     5101 cagacgcata aatgcaatat tgatattgcg gtcactcagg catttggtaa tataggccac
     5161 aacgccggga gtgtcctgat gaatcacgat cactgcactg tactcgcctg taaagtctac
     5221 ctgtacgtga ttgatatcta caatacggac cttgcctcct ccaagggatt cgccgcgtac
     5281 ggtcattttc tggccttttt cattttccat gcagatatct acggtgttcg gatgaatatc
     5341 tgtttcctgc tcatttggag tgaaagagaa tttcagacct ttttcatgag caatatcaaa
     5401 agaatttcgg atgcgcatat catcagtaga gaatcccata ataccaccca gcagagctcg
     5461 gtcagtaccg tgaccatggt aagtttttgc aaaagagccg tacaggatga agtcggctct
     5521 ctttaacggg ccttcgatca ttttctgggc aagataggaa atacgcgccg cacctgcggt
     5581 atgtgaactg gaagggccga tcatgttagg ccccataaca tcaaatacac tgataaatga
     5641 catatttttt ctcctgtgaa tatcaattat agtcttgcat gtttatggca ataatcttat
     5701 tatttgcaaa tttctgatta tattctatca tttaggaata aaagaagcaa acttatcgtt
     5761 aacattaaca aaaattaatg taaaaaatga gaaaacaggg ttgctttttc tctactgggc
     5821 ggttacaata actgtgcaaa ttgaaaataa ttttaaaaat gctgtttcaa aatagggaac
     5881 agcataaaat ttgctgttag aaatcagatt cccgaaaaca gggtctgata tccgaaatcg
     5941 taaattttaa agaaaaggca ggtggaacag aatggataat tgcgattctc atgggcgaag
     6001 tagtagcaca ggatctgaaa taaaaagagt atatgagtat agcagccgtt tttgttctgc
     6061 tgtagtcata tattttcagg attttccatg tatgcatata aaatagaagt tttttactgt
     6121 ctgtgatctg acagtttctg tatgtggaag atcggatgtc tttttcattt cttatcctgt
     6181 atttatgaaa ttcccccgaa tcatgcaatt acatattgag cagtaatgat ccggcgaaga
     6241 tgccggacag ttacggacag cggatgaata gcaggttatg gtttgatacc ctgcagttta
     6301 ttgtgctgga aaaagcatgg aagaaatggc agtaaaatgt aataaataac agactgcttt
     6361 cttttggtgt gcttttatgc aaaaaaacgg aggatgaaa
//
LOCUS       DBMDXG010000078         6309 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3142_length_6309_cov_2.255960, whole genome shotgun sequence.
ACCESSION   DBMDXG010000078 DBMDXG010000000
VERSION     DBMDXG010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3142_length_6309_cov_2.255960"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="ACZPD3_03550"
     CDS             <1..331
                     /locus_tag="ACZPD3_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1415112.1"
                     /translation="FTAIAGQNDMMAIGALDALHEARIHVPKDVSVIGCDNIFYSGIR
                     KISLTTIDHFVALKGRDACDIILRKIDEQDRFLTDSAPVSLYNIEYTPKLIARRTTGY
                     VRTKKNN"
     gene            760..1413
                     /gene="fsa"
                     /locus_tag="ACZPD3_03555"
     CDS             760..1413
                     /gene="fsa"
                     /locus_tag="ACZPD3_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR1415113.1"
                     /translation="MKFFIDTAKVEDIKAANDMGVICGVTTNPSLIAKEGRDFKEVIK
                     EITSIVDGPISGEVKATTTDAEGMIKEGREIAAIHPNMIVKIPMTVEGLKAVKVLHAE
                     GIKTNVTLIFTANQALLAARAGATYVSPFLGRLDDISTRGVDLIREIAEIFEVAGIET
                     EIIAASVRNPIHVTDCALAGADIATVPYNVIVQMTKHPLTDAGIEKFQKDYKAVFGE"
     gene            1429..2268
                     /locus_tag="ACZPD3_03560"
     CDS             1429..2268
                     /locus_tag="ACZPD3_03560"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR1415114.1"
                     /translation="MEKLELQKIANEVRKDIVTALHSAKAGHPGGSLSAADVFTYLYF
                     EEMNIDPKDPKKADRDRFVLSKGHTAPGYYSALAERGFFPKEDLKTLRHLGSYLQGHP
                     DMKHIPGVDMSSGSLGQGISAAVGMALSAKLSNESYRVYTLLGDGEIQEGQVWEAAMF
                     AGFRKLDNLVVIVDNNGLQIDGKVDEVCSPYPIDKKFEAFNFHVINVADGNDMDQLRA
                     AFDEAKATKGMPTAIIMKTVKGKGVSFMENQVGWHGKAPNDEQYAQAMEELEKAGEAL
                     CQK"
     gene            2256..3194
                     /locus_tag="ACZPD3_03565"
     CDS             2256..3194
                     /locus_tag="ACZPD3_03565"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR1415115.1"
                     /translation="MSEVKKIATRASYGAALVELGKKHENLVVLDADLAAATQTGVFK
                     KEFPERHIDCGIAECNMMGIAAGLATTGKVPFASTFAMFAAGRAFEQVRNSIAYPKIN
                     VKIGATHGGISVGEDGATHQCCEDFALMRVIPGMVVACPSDDIEAKAMVEAAYEHVGP
                     VYMRFGRLAVPVINDRPDYKFELGKGIVLREGKDLTIIANGLCVAPALEAAEKLAADG
                     VDAKVINIHTIKPLDEELVVAAAKETGKVVTVEEHSVIGGLGGAVCECLSEKAPVPVK
                     RIGVNDVFGESGPATALLEKYGLDAEGIYKQIKEFV"
     gene            complement(3167..3721)
                     /gene="yyaC"
                     /locus_tag="ACZPD3_03570"
     CDS             complement(3167..3721)
                     /gene="yyaC"
                     /locus_tag="ACZPD3_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HIR1415116.1"
                     /translation="MVFYVDSQKSSSAEEIAFLLNKCILQYPGRWSELVFLCIGSDRV
                     TGDCLGPYIGHQLLEHLNTDTHGVYVYGTLKSPVHALNLSRISRQIKILHPEGLVIAV
                     DASLGQKKHLGYVTIADGALYPGAAVQKELPPVGDIHITGIVNIAGVLEQLTLQTTRL
                     STVISLADTITQGIVNYTNSLICL"
     gene            4000..5367
                     /locus_tag="ACZPD3_03575"
     CDS             4000..5367
                     /locus_tag="ACZPD3_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR1415117.1"
                     /translation="MIEVIYKDESQEGQNGEEPFGLPRNIRQIGLAAEDYRIYMEDYV
                     YTFLVRLARTEDSLGEAKTRVAVLTGNLKWRSQTAYLFIKGAIIAEEMEAAPDHIDFS
                     ENQWKQIQEAQKEYFEDQEIVGWFFSQPQLLLKVSEVMLKVHMKHFGGEKVLMLMEPQ
                     EREDAFFRYENNEMVRLGGYYLYYEKNPGMQTYMIDKNEELQPEPQEKYEDQAVKDFR
                     KIIADKKETRKEPAAPSVFSYGLTACLAIAVLTVGVNFYRSYQNVKQNEKESATVSSV
                     IVEEITPSPVVGTSNNEAVRQHKQYRTDTIQNDAGKNKKNNEENDEENNQSTSEKENS
                     EKKNNTSEKKISAEQTDTEQKSDKTEQLPDVKTEKADKTEQIYQQEADERKAQKRVRE
                     AVQKENSEAAGKAHESYVIQPGDTLFQISMDRYGSIEAISQICKLNGMSADEIIYPGQ
                     VIVLP"
     gene            5448..>6309
                     /locus_tag="ACZPD3_03580"
     CDS             5448..>6309
                     /locus_tag="ACZPD3_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR1415118.1"
                     /translation="MANTFKKIIKKHKKKKLQAERNRKAKARERAEEEYADDYERRLS
                     RHKRSVVKKTVITVVAIAAAVTAVGFYIEKRSYHTYKVVQTSEQEDIVSTNYVEMDGN
                     ILRYSPDGVSLVSDKMSTLWSETYQMQNPVADVNGTRAVIADKDGTTLEIYDKSGKTG
                     SVTTSYSIVKAKVSKSGLVAAILDGGDDTWIDFYGTDGSLIAENQTKIDDPGYPLDIA
                     VSEDGVIMMVTYQFVDGSDTTSYVAFYNFGDVGQNEDDRIVSGYKYEGVVVPQIQYLD
                     NNRSVALKDNG"
BASE COUNT         2087 a         1107 c         1504 g         1611 t
ORIGIN      
        1 atttacagct attgcaggcc agaatgatat gatggctatt ggtgcgctgg atgctctcca
       61 tgaagcaagg attcatgtac ccaaggatgt atcggtgatt gggtgcgata atatctttta
      121 ttcgggaata cggaagatct ctcttactac gatcgatcat ttcgtagccc ttaaaggcag
      181 agatgcctgt gatattatac tgcgcaaaat cgatgagcag gaccgttttc tcacagattc
      241 tgctcctgtc agtctctata atattgaata tacccccaag ctgatagcga gacgcactac
      301 ggggtatgtc agaaccaaaa aaaataatta aatacgtcga atgaaaatta ttaataataa
      361 tttatcgaaa ctgcagaaca gttatttggt atagagctgt gtggcgaggt agattgcgaa
      421 tatctacccg gcaggcattt tctggaaacg taccaaaatt tcaggatgtg cggctgaaat
      481 ccgatatcag aaatgatttc tgacggaggt ttcctgcaac agcaaagaca gcgaacagtt
      541 acgaaaagaa atggttataa aacatattgt atattgcatg ttgataacaa cgaatatatg
      601 ctttgactga aaaataaaga attaataaga catgcaatta ttaataaaga aattattaat
      661 aatcttttgc cacgcttgac ctgaatgtaa agtctgttat aatgaaacca tcagatcaat
      721 gaacgattat gacaagcaaa aatcaggagg gttaagaaca tgaaattttt tattgacaca
      781 gcaaaagtag aagatattaa ggcagctaat gatatgggtg taatctgtgg tgttaccaca
      841 aatccgtctc ttatcgcaaa agaaggaaga gatttcaagg aagtaatcaa agagatcaca
      901 tctatcgtag acggaccgat cagcggtgaa gtaaaagcta ctacaacaga tgcagaagga
      961 atgatcaaag agggacgtga gattgcagcc attcatccga atatgattgt taaaattcct
     1021 atgacagtag aaggtctgaa agcagttaag gttcttcatg cagaaggaat caagacaaac
     1081 gttactctga tcttcactgc taaccaggca cttctggctg caagagcagg tgctacttat
     1141 gtatctccgt ttcttggaag actggatgac atttctacaa gaggtgttga tctgatccgc
     1201 gagatcgctg agatttttga agtggcagga atcgagacag aaatcatcgc agcaagcgta
     1261 agaaatccga tccatgtaac agactgtgca cttgcaggtg ctgacattgc aactgttccg
     1321 tacaatgtaa tcgtacagat gacaaaacat cctctgacag atgcaggtat tgagaaattc
     1381 cagaaagatt ataaagcagt attcggcgaa taaggagaat ttaccacaat ggaaaaatta
     1441 gaacttcaga aaattgcaaa cgaagtccgt aaagacatcg ttactgcatt acattctgca
     1501 aaagcaggac atccgggcgg atctctgtct gcagcagatg tattcactta tttatatttt
     1561 gaagaaatga atattgatcc aaaggatcct aagaaggctg atcgtgaccg ttttgtactt
     1621 tccaaaggcc acacagcacc gggatactac tctgcacttg cagaaagagg ctttttccca
     1681 aaagaagatc tgaagactct tcgtcatctg ggttcctatt tacagggaca tccggatatg
     1741 aaacatatcc cgggtgttga tatgtccagc ggttctctgg gacagggaat ctctgcagca
     1801 gttggtatgg cgctttctgc caaattaagc aatgaaagct atcgtgtata caccttatta
     1861 ggtgacggcg aaattcagga aggccaggtt tgggaagctg ctatgtttgc aggcttcaga
     1921 aaactggaca atcttgtagt gatcgtggat aataatggac ttcagatcga cggtaaggtt
     1981 gatgaagtat gttctccata cccgattgat aagaaatttg aggcatttaa cttccatgtg
     2041 attaacgttg cagacggaaa tgatatggat cagttaagag ctgcttttga tgaagcaaaa
     2101 gctactaagg gaatgcctac agctatcatt atgaaaactg taaaaggaaa aggtgtttcc
     2161 tttatggaaa atcaggttgg atggcatgga aaagctccga atgacgagca gtatgcacag
     2221 gcaatggaag agttagaaaa ggcaggtgaa gcattatgtc agaagtaaag aagatcgcaa
     2281 caagagcaag ctacggtgct gcgctggttg aattaggtaa gaaacatgag aacctggtcg
     2341 ttctggacgc tgaccttgct gcagctactc agactggcgt attcaaaaaa gaatttccgg
     2401 agcgtcatat tgactgtggt attgccgagt gtaatatgat gggcattgcc gcaggtctgg
     2461 ctactacagg aaaagttcca tttgccagca catttgcaat gtttgcagca ggacgtgctt
     2521 ttgagcaggt tcgtaactct attgcatatc cgaagatcaa tgtaaaaatt ggcgctactc
     2581 atggcggaat ttctgtaggt gaagatggtg ctacccatca gtgttgtgag gattttgctc
     2641 ttatgagagt aattccggga atggttgttg cctgcccttc tgatgatatt gaagcaaaag
     2701 ctatggtaga agcagcttat gagcatgttg gtcctgtata tatgagattt ggacgtctgg
     2761 cagttccggt tatcaatgac agaccggact ataaatttga attaggcaaa ggtattgtcc
     2821 ttcgtgaagg aaaagacctt acgattattg caaacggtct ctgtgtagct cctgctcttg
     2881 aagctgcaga gaagcttgcg gcagacggcg tagatgcaaa agtaatcaat atccatacga
     2941 ttaagcctct ggatgaagaa cttgtagttg cagcagccaa agaaacaggc aaggttgtaa
     3001 ctgtagaaga gcattcagta atcggcggac ttggcggagc agtatgcgaa tgcctgtctg
     3061 agaaagcacc tgtacctgta aaacgtatcg gtgtaaatga tgtattcgga gaatccggtc
     3121 ctgccacagc tcttcttgag aaatatggtc ttgatgcaga aggcatctac aaacagatca
     3181 aagaatttgt ataatttacg attccctgtg tgatggtatc tgccagagag attaccgtag
     3241 acaggcgggt agtctgaagg gttagctgtt ccagtacacc tgcaatgttt acaatccctg
     3301 tgatatggat atcaccaaca ggaggaagtt ctttctgaac tgcagcacct gggtaaagtg
     3361 caccatctgc gatggtcaca taccccaggt gctttttttg tccaagagag gcatccactg
     3421 caataaccag accttcggga tgcagaattt tgatctggcg ggaaattctg gaaagattta
     3481 aagcatgtac ggggcttttc agggtaccgt aaacataaac gccatgagtg tccgtattca
     3541 gatgttcaag taactggtgt ccgatatagg gaccgagaca gtctcctgtc acgcggtcac
     3601 ttccgataca cagaaaaacc agttccgacc atctgccggg atattgaaga atacatttat
     3661 ttaaaagaaa agcgatttcc tcagcagaac tgcttttctg tgagtccaca taaaatacca
     3721 taaaacctcc ctgatcgtat ttatgtactc gcagagtctc tattgtacct gcacaaaaag
     3781 atttcgagag cacattattg attatatctt ttaattgcta tagtcccttt gcttcataac
     3841 aacaagctgc gaagaggctt ctgataacat catatcctta ccgggataaa aatatgcact
     3901 gtgcaaaatc tgtcgccaaa ttctggtaaa ttcctcttac aatgtgataa aatctgcaat
     3961 ttatctgtgt tatcctgata cccgaagggg tgatgagaga tgatagaagt aatctataag
     4021 gacgaatctc aggaaggaca aaatggtgag gaaccgtttg gattacccag aaatatacgc
     4081 cagatcggcc tggcagccga agattacagg atttatatgg aggactatgt gtacacattt
     4141 ctggtaagac ttgccagaac ggaagactct ctgggagaag caaaaaccag agtggcagtt
     4201 cttacaggaa atcttaaatg gcgttctcag acagcttatc tgtttatcaa aggtgctatt
     4261 atagcggagg agatggaggc agcacctgat catatcgatt tttcggaaaa tcaatggaag
     4321 cagatacagg aagcccagaa ggagtacttc gaagatcagg agattgtggg atggttcttt
     4381 tcacagcctc agcttctcct gaaggtatcg gaagtgatgt taaaagtaca catgaaacat
     4441 tttggaggag aaaaagtcct gatgcttatg gaacctcagg aaagggaaga tgctttcttc
     4501 cgttatgaaa acaatgaaat ggtgcgtctg ggagggtatt atctttatta tgaaaaaaat
     4561 cccggtatgc agacgtatat gatcgacaaa aatgaagaac ttcagccgga acctcaggaa
     4621 aaatatgaag atcaggctgt gaaagatttt cgcaaaataa ttgcagataa aaaggaaacc
     4681 aggaaggagc cggcagctcc ttctgttttt tcatatggat taactgcatg tctggcaatt
     4741 gcagtactga cagtgggtgt taatttttac agaagttacc agaatgtaaa acagaacgaa
     4801 aaggagagcg cgacggtttc ttcagtgatc gttgaggaga taacgccgtc tcctgttgtc
     4861 gggacttcga acaatgaagc tgtcaggcag cataaacaat accggacaga tactatacaa
     4921 aatgatgcag ggaagaataa aaagaataat gaagagaatg atgaagagaa taatcaaagc
     4981 accagtgaaa aagaaaattc tgaaaagaaa aacaatactt cagagaaaaa aatatcagca
     5041 gagcagacag atacagaaca aaaatcggac aaaacagaac agcttccgga tgtaaagaca
     5101 gaaaaagcag ataaaaccga acaaatctac cagcaggaag cagatgaacg taaagcacag
     5161 aaacgtgtca gggaagctgt gcagaaggaa aacagcgaag cagcgggaaa agcccatgaa
     5221 tcatatgtga ttcagcccgg cgatactctt tttcagataa gtatggacag atacggaagt
     5281 atcgaagcca taagtcagat ctgcaaattg aatgggatgt cagcggacga aatcatttat
     5341 ccgggacaag taattgtatt accttagaaa actgtgctat aataaatggc aaaaactgaa
     5401 acctgataat caacaaaatg aaagtgagaa ataaggtaaa ggctaaaatg gcaaatacat
     5461 ttaagaaaat aataaaaaaa cataaaaaga aaaagcttca ggcagagaga aacagaaaag
     5521 cgaaagcccg ggagagagct gaagaggaat atgcagatga ttatgagcgc agattgtccc
     5581 gccacaaaag aagtgtagtg aaaaagacag ttattacagt ggtggcgata gctgctgctg
     5641 taacggctgt gggattttac atagagaaga gaagctatca tacttataaa gtagtccaga
     5701 caagtgagca ggaggatatt gtttctacaa attatgttga aatggacgga aatatccttc
     5761 gatacagtcc ggatggagtt tcgcttgttt ctgataagat gagtacatta tggagtgaaa
     5821 cctatcagat gcagaatcct gtggcagatg tcaatggaac tcgtgcggtg attgcggata
     5881 aagatggaac aactttggag atttatgata aatccggaaa aacaggcagt gtaactactt
     5941 cttacagtat tgtcaaagca aaagtatcca aaagcggtct tgttgcggca atcctggatg
     6001 gcggagatga tacatggatt gatttttatg gtacagatgg aagtctgatt gctgaaaatc
     6061 agacgaagat agatgatccg ggatatcccc ttgatattgc tgtatctgaa gacggagtga
     6121 tcatgatggt aacctatcag ttcgtagatg gaagtgatac gaccagttat gtggcatttt
     6181 acaattttgg agatgtagga cagaacgagg atgacagaat tgtcagtgga tataagtatg
     6241 aaggcgtggt agttccgcag atccagtatc ttgataataa ccggtctgta gcacttaaag
     6301 ataatggat
//
LOCUS       DBMDXG010000079         6292 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3154_length_6292_cov_1.605718, whole genome shotgun sequence.
ACCESSION   DBMDXG010000079 DBMDXG010000000
VERSION     DBMDXG010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3154_length_6292_cov_1.605718"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /gene="ribE"
                     /locus_tag="ACZPD3_03585"
     CDS             complement(<1..481)
                     /gene="ribE"
                     /locus_tag="ACZPD3_03585"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR1415119.1"
                     /translation="MFTGIVEELGTVKKIKKGANSAVFTIRAEKILDDLKTGDSVAVN
                     GICLTVTACLEDGFTADVMHETLNRSALIQLSLGQHVNLERAMPANGRFGGHIVAGHV
                     DGTGKITEIRRDDNAVWYTIQASPQIMKYIVTKGSVTVDGISLTVAKVSETDFSISAI
                     "
     gene            complement(462..1571)
                     /gene="ribD"
                     /locus_tag="ACZPD3_03590"
     CDS             complement(462..1571)
                     /gene="ribD"
                     /locus_tag="ACZPD3_03590"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR1415120.1"
                     /translation="MEKDRQYMKMALELAQKGMGFTAPNPMVGAVIVKSGKVIGQGYH
                     RKYGELHAEREALASCTEQPEGASIYVTLEPCCHYGKQPPCVNAILESGIRRVIIGSS
                     DPNPLVSGKGIRILKEHGIEVTENVLKEECDKLNEAFFYYIQNQKPYVVMKYAMTMDG
                     KIATYTGASRWVTGEAARMHVQKQRLKYTGIMAGVGTVLADDPMLTCRLENGRNPVRI
                     ICDSHLRTPLNSRIVKTASTIPTIFATSSKDQQKIKNYEDMGCKVLDVPEKNGHIDLN
                     RLMELLGAAKIDSILLEGGGTLNWSALESGIVQKVQTYIAPKLFGGESAKTPIEGKGF
                     FDPASAILLKNSEIIRIGDDFLIESEVKSNVHGNC"
     regulatory      complement(1816..1930)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(2037..3527)
                     /locus_tag="ACZPD3_03595"
     CDS             complement(2037..3527)
                     /locus_tag="ACZPD3_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1415121.1"
                     /translation="MPVFDFSGASDKKTGVQSECTYTTFQSNEKETSPEKPIMILDNS
                     SRQWKHHSCGIFTNPIKRTSFEFKEDDGVFSADIISIDSRFVSLLKWLGENHINVRLS
                     GENKENGYAVYKIRETAFGGGTKLSAEDGFLQFMIERLLASSAPAEIVEDEDEEETGD
                     EMKLTSIQSITDFMTCAGRTLPDNIRLWARRNLAVARSHEVSPEERRHAQRALSIMMN
                     VQWKSNYFEAIDPQEARRILDEELYGMESVKQRIIETIIQINRTHTLPAYGLLLVGPA
                     GTGKSQIAYAVARILKLPWTTLDMSSINDPEQLTGSSRIYANAKPGIIMEAFSAAGES
                     NLVFIINELDKAASGKGNGNPADVLLTLLDNLGFTDNYMECMVPTVGVYPIATANDKS
                     QISAPLMSRFAVIDIPDYTSEEKKIIFSKYVLPKVLKRMSLKAEECVVTEDGLDAIVE
                     LHKNTSGIRDLEQAAEHIAANALYQIEVDHLTGVTFNAEMVRELLS"
     gene            3799..4860
                     /gene="trpS"
                     /locus_tag="ACZPD3_03600"
     CDS             3799..4860
                     /gene="trpS"
                     /locus_tag="ACZPD3_03600"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR1415122.1"
                     /translation="MSKIILTGDRPTGRLHVGHYVGSLSERVRLQNSGLYDEIYIMIA
                     DAQALTDNAEHPEKVRQNILQVALDYLACGIDPEKSTIFIQSMVPELTELTFYYMNLV
                     TVARVQRNPTVKSEIQMRNFEASIPVGFFCYPISQAADITAFRATTVPVGEDQLPMLE
                     QCKEIVHKFNTVYGETLTEPEIILPSNKACLRLPGIDGKAKMSKSLGNCIYLSDEEAD
                     VKKKVMSMFTDPNHLRVEDPGRVEGNPVFIYLDAFCKPEYFAEFLPEYQNLDELKAHY
                     TRGGLGDVKVKKFLNKVLQAELSPIRERRKYWEQHLDDVYDILKEGSKVAEAKAAQTL
                     HDVRSAMQINYFDDNSLIK"
     gene            complement(5059..>6292)
                     /locus_tag="ACZPD3_03605"
     CDS             complement(5059..>6292)
                     /locus_tag="ACZPD3_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lacto-N-biose phosphorylase central
                     domain-containing protein"
                     /protein_id="HIR1415123.1"
                     /translation="FMGDHWIGMEPFMDEFASIGLDAVVGSVGNGATLRLFSDIKNVK
                     YTEGRFLPYFFPDTFHEGGDPVKEAKVNWVTARRAILRSPIQRIGYGGYLKLALEFPD
                     FVQYIKEVCEEFRTLYDNIQGTTPYCVKRVAVLNCWGKMRSWGNHMVHHAIYYKQNYS
                     YFGIIEALSGAPFDVSFISFDDIKADKDLLKKFDVVINVGDADTAQSGGENWIDETII
                     TAVREFVYNGGGFIGVGEPAAHQWQGRFIQLDDVLGVEEEHGFNLNTDKYNWEEHRDH
                     FILKDAEGEVDFGEGKKNIYALPETEILIQKDQEVQMAVKTFGKGRGVYISGLPYSFK
                     NSRVLYRAVLWSASAEEELHCWYSTNYNVEVHAYVKNGKYCVVNNTYEPQDTVVYRGD
                     GSSFRLHMEANEIKWYQI"
BASE COUNT         1632 a         1469 c         1179 g         2012 t
ORIGIN      
        1 gaattgcaga aatggaaaag tctgtttctg agacttttgc cactgtaaga ctgattccat
       61 caactgttac agaacctttt gtcacaatat atttcataat ttgtggtgaa gcctgaatgg
      121 tgtaccagac agcattatca tctctgcgaa tttccgtgat ttttcctgtt ccatccacat
      181 gcccggcaac aatgtgtcct ccaaatcttc catttgcagg cattgccctt tccagattta
      241 catgctgtcc caaagaaagc tgaatcagcg cagaacgatt caatgtctca tgcatcacat
      301 ctgctgtaaa accatcttcc agacatgcag ttacagtgag gcagattcca tttacagcta
      361 cactgtcccc tgttttcaga tcatccagaa ttttctctgc ccgtatcgta aatactgctg
      421 aatttgctcc tttttttatt tttttcacag ttcccagttc ttcaacaatt cccgtgaaca
      481 ttactcttca cctcactctc tataagaaaa tcatctccta ttctgattat ttcgctgttt
      541 tttaataata ttgcgctggc aggatcaaaa aatccttttc cttctatcgg tgtctttgca
      601 ctttctcccc caaaaagttt gggtgctata taggtctgga ctttctgaac aataccgctt
      661 tcgagtgctg accagtttaa agtgcctcct ccttccagta agatactgtc tatctttgca
      721 gctcccagaa gttccattag tctgtttaaa tctatgtgtc cgtttttttc cgggacatcc
      781 agaactttac atcccatatc ttcataattc tttatcttct gctggtcttt actgctggtt
      841 gcaaagattg ttggaattgt tgatgccgtt ttcacaattc tggaattcag tggtgtccgc
      901 aagtgggagt cacagataat acgcacagga tttctgccgt tttccagtct gcatgtaagc
      961 atcggatcat ctgccagcac agttcccact ccagccataa tccctgtata tttcaatctc
     1021 tgtttctgca catgcattct ggcagcttcg cctgtcaccc atctggatgc gcctgtataa
     1081 gtggcaattt ttccatccat ggtcatcgca tatttcatga ctacatatgg tttttgattc
     1141 tgtatgtaat agaaaaatgc ttcattgagt ttatcgcact cttcttttaa aacgttttcc
     1201 gttacttcga tcccatgctc tttcagaatc cggattcctt ttcctgaaac caggggatta
     1261 gggtccgagg aaccaataat cacacgtctg atccccgatt ccaggattgc attgacacac
     1321 ggaggctgtt tcccgtaatg gcagcaaggt tccagagtaa catatatgga ggcaccttct
     1381 ggctgttctg tacatgatgc cagagcttcc ctttctgcat gaagttcacc gtattttctg
     1441 tgatatccct ggccaatgac ttttccgctt ttgacgatca cggcacctac cattgggtta
     1501 ggtgctgtaa atcccattcc tttttgcgcc agttctaacg ccattttcat atattgccta
     1561 tctttttcca tatccgttat atctttcaat ccctcatctc ctgttcttct gatggtactg
     1621 atgataccaa aaaagccccg gtgtctttca acatccaggg cctgtaaatt aatatctgct
     1681 tgaattatct tcgtgtgaat ctgacttttt gcttcatcat taaaactgct ttgatgatat
     1741 taaaaagctc tggaataaat ttattattcc agagcagtcg ctttcacaga tcagttaatt
     1801 cttctgtatc tctactgtta tcttctttca tccagactgt actgtcggct tcggaattac
     1861 accgaatcat gccttacggc tcgtgggctt taccaccggt agggaattgc accctgccct
     1921 gaagatctta ttttattttt atcagcaatt tctaaatttt atcctgttga tgtctgaaag
     1981 tatactctta atttttattt atgtcaagat tttttcagac atataatttc agtttttcag
     2041 gaaagtaatt ctcttaccat ctctgcattg aatgtcacac ctgtcaggtg atctacttca
     2101 atctgataca gggcattggc tgcgatatgc tctgctgcct gttccagatc acgaataccg
     2161 cttgtatttt tatgcaattc aacgatggca tccagaccgt cttctgttac tacacattct
     2221 tctgctttca ggctcatgcg tttcagaact ttcggaagaa cgtatttgga gaagatgatc
     2281 ttcttctctt ctgatgtata atccgggata tcgatcaccg cgaaacggga catcagaggt
     2341 gcgctgatct gggatttatc gtttgccgta gcaataggat aaacgcccac tgtgggcacc
     2401 atacattcca tatagttatc tgtaaatcca aggttgtcaa gcagagtaag aagtacgtct
     2461 gcaggatttc cgttgccctt acctgacgct gctttatcaa gctcgttgat aataaataca
     2521 aggttggatt ctcctgctgc cgagaaagct tccatgatga ttccgggctt tgcgtttgca
     2581 tagattctgg aacttcctgt aagctgttcc ggatcattga tggaactcat gtccagagtt
     2641 gtccaaggca gtttcagaat acgggcaact gcataggcaa tctgggattt gcctgtacct
     2701 gcaggaccta caaggagaag tccgtatgca ggaagtgtat gggtacggtt gatctgtatg
     2761 attgtttcaa tgatacgctg ctttacagat tccattccgt agagttcctc atcaaggatc
     2821 ctgcgggctt cctgaggatc gattgcttca aaataattgc tcttccactg tacattcatc
     2881 atgatggaaa gtgctctctg agcatgacgt ctctcctccg gagatacttc gtgtgaacgg
     2941 gcaactgcaa ggtttcttct cgcccagaga cggatattat ccggcagggt tctgcctgcg
     3001 caggtcataa aatcagtgat gctctgaatg cttgtcagct tcatctcgtc ccctgtttct
     3061 tcctcatctt catcctcaac aatctctgca ggtgcactgc ttgcaagaag tctctcgatc
     3121 ataaactgaa gaaatccgtc ctctgcggaa agtttcgtac cgccgccaaa agcagtttca
     3181 cgaatcttat aaactgcata accattctct ttattctctc cagaaagacg cacattgata
     3241 tgattctccc caagccattt cagaagactt acaaatcttg aatcaatact gataatatct
     3301 gcactgaaaa cgccgtcatc ttctttaaat tcaaaggatg tacgtttaat cggattagta
     3361 aaaataccgc aggaatgatg tttccactga cggctgctgt tatccaggat catgatcggt
     3421 ttctcagggg aagtctcctt ttcattggac tggaacgtag tgtaggtaca ttcagactgt
     3481 acgcctgttt tcttgtctga agcaccgctg aaatcaaata ctggcatggt aaatactcct
     3541 tttatcttat catgcaatgc atgacatatt tctattacac atctctgcaa tcctgtcgga
     3601 agctatttat tattctagca gaatcttttc ttttaatcaa ttctgtaaat gtatttattg
     3661 cataaatatt caataaatta tatcctgatt tattccccca aaatcattga cggtaacatt
     3721 cacaggcctt ataataatag taactgagaa tttccagaaa ctctctggaa aatcggaatt
     3781 tacttttagg agagcaaaat gagcaaaatt attttaaccg gtgaccgccc aacaggccgc
     3841 cttcatgttg gacattatgt tggttcatta tccgagcgtg ttcgtttaca gaactccggc
     3901 ttatatgatg aaatttatat tatgatcgca gatgcacagg cattaacaga taacgctgaa
     3961 caccctgaga aagttcgcca gaacatcctt caggtcgccc tggattatct tgcatgcggc
     4021 attgatcctg agaaatccac tatctttatc cagtccatgg ttccggagct tacagagctt
     4081 actttctatt atatgaacct tgttactgtg gcacgtgtac agcgtaatcc tacagttaaa
     4141 tctgaaatcc agatgcgtaa ttttgaggca agcatccctg ttggattctt ctgctatccg
     4201 atcagccagg ctgcagacat cactgcattc cgcgctacta ctgttccggt tggcgaagat
     4261 cagcttccaa tgctcgagca gtgcaaggag atcgtccaca aatttaatac tgtttatggt
     4321 gaaactctga cagagcctga gattattctg ccatccaata aagcatgctt acgtctgcca
     4381 ggtattgacg gaaaagcaaa aatgagcaaa tctctcggaa actgcatcta cctttcagat
     4441 gaagaagctg atgttaagaa gaaggttatg tccatgttca cagacccgaa tcatcttcgt
     4501 gtggaagatc cgggacgtgt ggaaggaaat cctgtattta tttatctgga tgcattctgt
     4561 aaaccggaat atttcgcaga gttcctgcct gaatatcaga atctggatga actgaaagct
     4621 cattatacac gcggcggtct gggtgatgtc aaggttaaga aattcctgaa caaagtactc
     4681 caggcagagc tttctccaat ccgcgaaaga cgtaaatact gggaacagca tcttgatgat
     4741 gtatatgata tcctcaaaga gggaagcaag gttgctgaag caaaagctgc acagacactt
     4801 catgatgttc gttcagcaat gcagatcaac tactttgatg ataacagctt aattaaataa
     4861 ttatatgccg gtggctacag cgaccgacaa cgatgcgtaa ataagaaatt caaacgcaag
     4921 attgttcaaa aatcaactta ttgacacatc aaaacaggtt caggcaaaag cattctgctc
     4981 acgtctgaac ctgttctgtt atttcttcac tgacttgctt ttcaatctat tttacagata
     5041 aaatctatcc gatatctttc atatctgata ccatttgatc tcatttgcct ccatatgtaa
     5101 ccggaaactg cttccatcac ctctgtaaac tactgtatcc tgtggttcgt aagtgttgtt
     5161 gactacgcag tattttccat tttttacgta agcatgtact tctacattgt agttggttga
     5221 ataccagcag tgaagttctt cctctgcgga tgctgaccaa aggactgcac gataaagcac
     5281 gcggctgttc ttgaagctgt atggaagtcc gctgatataa acgcctcttc ctttaccgaa
     5341 agtctttaca gccatctgca cttcctggtc tttctggatt aagatttctg tttccggtaa
     5401 ggcataaatg ttcttcttac cttcaccgaa atcaacctcg ccttctgcat ctttaaggat
     5461 gaaatgatct ctgtgttctt cccagttata tttatctgta ttcaggttaa atccatgttc
     5521 ttcttctact ccaagtacat catctaactg aatgaatctg ccctgccact gatgtgctgc
     5581 cggttctcct acaccgataa atccaccgcc attatataca aattctctga ctgctgtgat
     5641 gattgtttcg tcgatccagt tctcgccgcc tgactgtgca gtgtccgcat ctcctacatt
     5701 gatcactacg tcaaacttct tcagaagatc tttgtctgct ttgatatcat caaagctgat
     5761 aaatgataca tcaaacggtg cgccgcttaa tgcttcgata attccgaaat aagaataatt
     5821 ctgcttgtaa tagatggcgt gatgtaccat atggttaccc catgagcgca tctttcccca
     5881 acaattcagg actgctaccc gttttacgca ataaggagtc gttccctgaa tgttatcata
     5941 taaagtgcgg aactcttcgc agacttcttt gatatactgt acaaaatccg ggaattccag
     6001 ggcaagtttc agatatccgc cgtagcctat tctctggatc gggcttcgaa ggattgctct
     6061 tcttgctgtt acccagttta cttttgcctc ctttacagga tcgccgcctt catggaacgt
     6121 atccgggaaa aagtatggca aaaatcttcc ttctgtatat tttacattct taatatcact
     6181 gaacagacgc agggtcgcac catttccaac acttccaact acagcatcca gtccgataga
     6241 tgcaaattcg tccataaacg gttccatacc aatccagtga tctcccatga ac
//
LOCUS       DBMDXG010000080         6248 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3186_length_6248_cov_1.612982, whole genome shotgun sequence.
ACCESSION   DBMDXG010000080 DBMDXG010000000
VERSION     DBMDXG010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_6248_cov_1.612982"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(253..1023)
                     /locus_tag="ACZPD3_03610"
     CDS             complement(253..1023)
                     /locus_tag="ACZPD3_03610"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HIR1415124.1"
                     /translation="MYTVDLNSDLGESFGNYKIGNDDKVIPLISSANVACGYHASDPV
                     VMGNTLAMAREAGIRIGAHPGFPDLMGFGRRNLSVSPAEAKAYVLYQLGALDAFCRVT
                     GVKMQHVKPHGALYNMAAEDYALSTAICEAIKEFDSSLIVLALSGGQLAKAAQDMGLR
                     TAMEVFADRAYEEDGTLVNRRKEGAVITDENEAIVRVIRMVKEKKITAITGKDIDIQA
                     DSVCVHGDGAKALAFVEKIREAFAKEGIQIRSLDEFVK"
     gene            complement(1110..2459)
                     /gene="accC"
                     /locus_tag="ACZPD3_03615"
     CDS             complement(1110..2459)
                     /gene="accC"
                     /locus_tag="ACZPD3_03615"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR1415125.1"
                     /translation="MFNKILIANRGEIAVRIIRACRNLGIRSVAVYSKEDAKSLHVQL
                     ADQRICIGEGPARNSYLNMDRIIAAAENMGADAIHPGFGFLSENSEFVRKCKENGITF
                     IGPDADVIDKMGNKSHARKTMMDAGVPVVPGTKEPVYDAETGKKLAEEIGYPVMIKAS
                     SGGGGKGMRVAANEDEFEFQFNMAQRESANAFGDDTMYIERFVENPRHVEIQIMADSH
                     GNVVALGERDCSVQRNHQKLIEESPSPAIDEETRRKMNEYAVLAAKTVGYTNAGTIEF
                     IVDPKGNFYFMEMNTRIQVEHGVTEMVTGTDLIIEQIRVAMGEELSFKQEDIRIKGHA
                     IECRINAEIPEKNFMPSPGVVQHMHLPAGNGVRVDTALYTGYKIPSEYDSMIAKVIVH
                     APDREAALQKMRSALDEMVVMGVETNLDFQYQIMKNQVFCDGKADTGFIENVLKIKG"
     gene            complement(2478..2915)
                     /gene="accB"
                     /locus_tag="ACZPD3_03620"
     CDS             complement(2478..2915)
                     /gene="accB"
                     /locus_tag="ACZPD3_03620"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR1415126.1"
                     /translation="MDLEKIEGLVKIIEDSSLNEFTYKDKDVKITMSKLDHPPVVAAG
                     VPVAASAPVNTIVEKAEEEEEESLFITSPIVGTFYSASAPDVPAFVKVGDQVKAGQTV
                     CILEAMKLMNEIQSDYDCEIEAVLVSNEQKVEYGQPLFRVKRL"
     gene            complement(2958..3965)
                     /locus_tag="ACZPD3_03625"
     CDS             complement(2958..3965)
                     /locus_tag="ACZPD3_03625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit C family protein"
                     /protein_id="HIR1415127.1"
                     /translation="MGIRILKGGMMTTVQDLGRYGYQSQGFSVAGVMDVRSFKIANLL
                     LDNPENEAVLEITLIGPTLEFTSATIIAITGGDFQPTINGEPAPMYTALYMNKGDVLK
                     FGSARTGSRGYIAFSSYLDIPVVMGSRCTNLKSGLGGFKGRKLEADDYIGFRIKRRYL
                     PFFLSRKLDMDEFDQTEATLRVVMGPQDGMFSKQGIKTFLGSEYTVTNEFDRMGCRLE
                     GPFIAPKKTSDIISDGIAFGAIQVPSHGKPIILLADRQTTGGYGKIATVASVDIPKLV
                     QRKTDDKIHFKAITVQEAQALYVEEMKELDGLRKIIHQPCKEVLDCRLVAKRLRKLFE
                     E"
     gene            complement(3967..4692)
                     /gene="pxpB"
                     /locus_tag="ACZPD3_03630"
     CDS             complement(3967..4692)
                     /gene="pxpB"
                     /locus_tag="ACZPD3_03630"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HIR1415128.1"
                     /translation="MPDIRILTEGDSSVLVEFGKEISPEINRKITATVQLMKEQHIEG
                     VVDMIPAFCSLLVNYDPRVISYDDLKKRLEILLKMEVTAGEGCRKVYEIPVCYGGEYG
                     PDIENIAEHAGLSVEEVIKIHSSRDYLIYMLGFLPGFCYLGGLDERIHTPRLANPRIK
                     INAGSVGIGGSQTGIYPLDSPGGWQLMGMTPVKTYDPDREIPILVEAGDYIRFVPIDE
                     DEYKRIRELVERGEYQCTVHEEA"
     gene            complement(4846..6048)
                     /locus_tag="ACZPD3_03635"
     CDS             complement(4846..6048)
                     /locus_tag="ACZPD3_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HIR1415129.1"
                     /translation="MSEKKKSGGALLGAAFLMATSAIGPGFLTQTATFTGQYQESFGF
                     VILVSVILAAIAQLNIWRVLCVTGLRGQDVSNKVLPGLGYLVSILIVFGGLVFNIGNV
                     GGGALGFNTLLGIPTKVGYILAGLLAILVFVLKNAKSAMDTITKVLGAIMIIVIFVVI
                     IVVKPPVGSALKNTFVPEAGATNLIPAILTLLGGTVGGYITFSGAHRLIDAGITGEKN
                     LKEINKSSVMGMIIATIVRIFLFLAVLGVVVKGVTLDAANPAADAFKQGAGEIGYRFA
                     GLVLLCAAITSIIGAAYTSVSFLKTFSKSIEENENKVIIGFIIISTAIMFILGNPAVL
                     LVLAGAVNGLILPITLAICLIAAHKKSIMGENYHHPVVLTILGVIVVILTAYLGVTTF
                     VSKIGTLL"
BASE COUNT         1677 a         1612 c         1124 g         1835 t
ORIGIN      
        1 atttcaaaac ctatacaaaa catgagcttg gcacttatca cgattatgac ctgtgtctgg
       61 ataccggaac ccttggatac gataactgta ttgagattat cacttcactt gcaaaacagc
      121 aggggttgaa tctgaagcat cagtagaagg accgctgcca gctgtaaaac agcaacaagg
      181 ctgtggcaat cagaaaatct gtgccacagc ctctttactt cagtcctttt aatagttgtt
      241 tctaaatttc ttttatttta caaactcatc cagagaacgg atctggattc cttcttttgc
      301 aaaagcctct ctgatttttt ccacaaacgc cagagccttg gctccgtctc catgaacgca
      361 gacagaatct gcctgaatat caatatcttt tcctgtaatt gcagtaatct ttttctcttt
      421 gaccatacgg atcaccctga caatagcctc attttcatca gtgatcacag caccttcctt
      481 acgtctgttt accagagtac catcttcttc ataggcgcgg tctgcaaaaa cctccatagc
      541 tgtacgcagg cccatatcct gagcagcctt tgccagctgt ccgccggaaa gagcaagtac
      601 gatcagactg ctgtcaaatt ctttgattgc ctcacagatt gcagtagaaa gggcatagtc
      661 ctctgcagcc atattatata aggctccatg aggctttaca tgctgcattt ttacaccggt
      721 tactctgcag aatgcatcca gtgcacccag ctgataaagc acatatgctt ttgcctctgc
      781 aggggaaaca ctcagatttc ttcttccgaa gcccatcaga tcaggaaaac ccggatgtgc
      841 tcctatacga attcccgctt cccttgccat tgccagcgta tttcccataa ctaccggatc
      901 tgaagcatga taaccgcagg ctacatttgc agaagagatc agcggaatga ccttatcatc
      961 attaccgatt ttatagtttc caaaactttc tcccagatca ctgtttaaat ctactgtata
     1021 catttacctt acctcttttc tatttatttc ccagagcgtg tttgaaaaat cactccctca
     1081 atccgtatac aggatcctta cctacaatct tatcccttaa ttttcagtac attctcaata
     1141 aatcccgtat ctgcctttcc gtcgcagaat acctgattct tcataatctg atactggaaa
     1201 tcaaggtttg tctccacacc catgaccacc atctcatcca gcgcagagcg cattttctgc
     1261 agggcagcct ctctgtcagg tgcatgaacg attaccttcg caatcatgga atcatactct
     1321 gacggaatct tatagcctgt ataaagggca gtatccaccc ggactccgtt gcctgccgga
     1381 agatgcatat gctgtactac tccgggactt ggcatgaaat ttttctcagg aatctctgcg
     1441 ttgatacggc attcaatagc atgtccttta attcggatat cttcctgttt aaagcttaac
     1501 tcctctccca tagccacacg aatctgctca atgatcagat ctgtgcctgt aaccatctct
     1561 gttactccat gctccacctg gattctggta ttcatttcca tgaaatagaa gttgccttta
     1621 gggtctacga taaattcaat cgttcctgca ttggtataac ctactgtttt cgcagcaaga
     1681 actgcatatt cattcatttt cctgcgagtt tcttcatcaa tggcagggga cggggattcc
     1741 tcgatcagct tctgatgatt tctctgtacc gaacagtcac gttctcccag tgccaccaca
     1801 tttccatgac tgtctgccat aatctggatc tccacatggc ggggattttc tacaaaacgt
     1861 tcaatgtaca tggtatcatc cccaaaagca ttggctgatt ctctctgtgc catattaaac
     1921 tgaaattcaa attcatcctc attggcagcc acacgcattc ccttgccacc accgcctgag
     1981 gaagccttga tcataaccgg atatccaatc tcttcagcca gttttttacc tgtttctgca
     2041 tcgtatacag gttcctttgt tcccggtaca accggtaccc cggcatccat catggtttta
     2101 cgggcatggg atttatttcc catcttatcg atcacatccg catccggtcc gataaaggta
     2161 attccgtttt ctttgcattt gcggacaaac tcactgtttt ctgaaagaaa gccaaaaccg
     2221 ggatggatag catctgctcc catattttct gctgcagcaa tgatccggtc catgttcagg
     2281 tagctgtttc tggcaggacc ttctccgata cagatacgct ggtctgccag ctgtacatga
     2341 aggctcttag catcttcttt tgaataaaca gctacacttc ggatccccag attcctgcag
     2401 gcacgtatga tccgaactgc aatctcccca cggttggcga tcaaaatttt attaaacaat
     2461 atgaaagcct ccttatccta cagtctctta acacggaaca gaggctgtcc atattctact
     2521 ttctgttcat tacttaccag aactgcttca atctcacagt catagtcact ctggatctca
     2581 ttcataagct tcatagcttc cagaatacac actgtctgtc ctgcttttac ctgatctccc
     2641 acctttacaa aagcaggtac atccggagca gatgcagaat agaaggttcc tacgatagga
     2701 gaagtgataa acagactttc ctcctcttct tcctctgctt tttccacaat tgtattcaca
     2761 ggtgcagatg cagccacagg aactcccgct gcaactacag gaggatgatc cagcttgctc
     2821 attgtaatct ttacatcctt gtccttatat gtgaattcat taagggatga atcctcaata
     2881 atctttacca gtccttcaat cttctctaaa tccatcaaac attatccttt ctgtctattt
     2941 tatattattt tttacggtta ttcttcaaac agcttgcgca gtctctttgc caccagacgg
     3001 caatccagaa cctctttaca tggctgatga atgatctttc tcagtccgtc aagttctttc
     3061 atttcctcaa cataaagagc ctgagcctcc tgtacggtaa ttgccttgaa atgtatctta
     3121 tcatctgttt ttctctgtac cagcttggga atatccacag atgccactgt ggcaattttc
     3181 ccataaccgc ccgttgtctg acggtcagcc agcagaatga taggtttacc atgggaaggt
     3241 acctgtatag ccccaaatgc gattccgtcg gaaataatat ccgaagtctt cttaggagca
     3301 ataaacggtc cctccagcct gcagcccata cggtcaaatt catttgtcac tgtatactca
     3361 ctgcccagga aagtcttgat gccctgtttg ctgaacatac catcctgcgg tcccattact
     3421 acccgcagtg ttgcttctgt ctgatcaaac tcatccatat caagctttcg ggaaaggaaa
     3481 aacggcagat aacgtctctt aatacgaaat ccgatataat cgtccgcttc cagttttctt
     3541 cccttaaatc cgcccagacc actctttaaa ttggtgcatc ggcttcccat cactaccgga
     3601 atatccagat aactggaaaa agcaatataa cctctgcttc ccgttctggc gcttccaaat
     3661 ttcagtacat ctcccttatt catatacagg gctgtataca tgggagccgg ctctccatta
     3721 atggttggct ggaaatctcc gcctgtgatt gcaatgatag tagctgatgt aaattcaagg
     3781 gttgggccta tcagcgttat ctccaatact gcctcattct ccggattatc cagaagaaga
     3841 ttggctattt tgaatgaacg gacatccatg actcctgcca cactgaatcc ctgactctga
     3901 tatccataac gccccagatc ctgaaccgtt gtcatcattc cgccttttaa tatacgaatt
     3961 cccattttat gcctcctcat gaacggtgca ctggtattcg ccacgttcta ctaattctct
     4021 gatccgtttg tactcatctt catcgatcgg tacaaaacgg atataatctc ctgcctctac
     4081 cagaatcggg atttcacggt ccggatcata ggtttttaca ggtgtcattc ccatcagctg
     4141 ccatcctccc ggagaatcca gaggataaat ccctgtctgg gaaccaccga ttcccacact
     4201 tcctgcattg atcttgattc tgggatttgc aagacggggt gtatggatcc tttcatccag
     4261 cccgcccaga taacaaaagc ccggaagaaa accaagcata tagatcaggt aatctctgga
     4321 agaatgaatc tttattactt cctctacgga aagtccggca tgttctgcaa tattctcaat
     4381 atccggacca tattctcctc catagcatac aggaatctca taaacttttc tgcatccttc
     4441 acctgctgta acttccatct ttaacagaat ttccagtctt tttttcaggt catcgtagga
     4501 aatcacacgc gggtcataat taaccagcaa agaacagaac gcagggatca tatctacaac
     4561 tccctctata tgctgttctt tcataagctg tacagtggca gtgatcttcc ggttgatctc
     4621 cggactgatc tctttgccaa attcaaccag aacagaagaa tcaccttctg ttaaaattct
     4681 aatgtccggc ataatctgct cctcttttct taatcttttc gccatgtctt tttatctgct
     4741 tctttctgca tcacatggct gttaatgaac caaaaaggtg taacctggac gatcacacct
     4801 ttttgacata ctctccagtc tgaaaaccgg atacattttc ctcaattata acaatgtacc
     4861 gatcttagat acaaatgtag tcacaccgag gtatgctgta agaattacca cgatcactcc
     4921 aagaatggta agtactaccg gatgatgata attctctccc ataatagatt tcttatgtgc
     4981 agcgatcaga cagattgcca gggtgatcgg aaggatcaga ccattcacag cacctgcaag
     5041 tacaagaagt actgccggat ttccaaggat aaacataatg gctgtagaaa taatgataaa
     5101 tccgatgatc actttatttt cattttcctc gatagacttg ctgaaagtct tcaggaagga
     5161 aactgaagta taagctgcac cgataatgga tgtgattgct gcgcacagaa gaaccagacc
     5221 tgcaaaacgg tatccgattt caccagcccc ctgcttgaag gcgtctgctg caggatttgc
     5281 agcatccagt gttacgccct tgacaacaac gcccagaaca gccagaaaca ggaaaatacg
     5341 aacaatcgta gcaatgatca tacccataac agaactcttg ttgatctctt tcaggttctt
     5401 ctcgcctgta attcctgcat caatcagacg atgtgcgccg gagaaagtga tatatcctcc
     5461 aacagtacct cccaaaagtg taaggattgc aggaatcaga tttgtggcac cggcttccgg
     5521 tacaaatgta ttcttcaatg cacttcctac cggtggtttt accacaatga tcacaacaaa
     5581 gattacgata atcataatgg cacccagtac cttggtaata gtatccatgg cacttttggc
     5641 atttttcagc acaaatacca ggattgccaa aagacctgcc agtatgtaac caactttggt
     5701 aggtattcca agcaatgtgt taaaaccaag tgctccgcca cctacgtttc cgatattaaa
     5761 taccagacca ccgaatacga tcagtatcga taccagatat cccagtcccg gaagtacctt
     5821 attggaaaca tcctgaccac gaagtccggt cacacaaagc acacgccaga tattcagctg
     5881 tgcaatggct gcaaggatta cggaaaccag gataacaaat ccaaaacttt cctgatactg
     5941 tccggtaaac gttgctgtct gtgtcaggaa tcctggtccg attgcagatg tggccatcag
     6001 aaacgctgcg ccaagtaagg ctccgccact ctttttcttt tcgctcattt ttattgcctc
     6061 cttttcatgt tattatcgca cattatacta aaaaatgaat atttacacaa gcaaaaatta
     6121 ttttgataac ttttgatgtc gaatttttgt tactgttcac tggtaatatc ccgcgaggtg
     6181 ttttttgtga gcgaaggtca cagaaaaccc gagacatact gcgcgaattt atgcgattag
     6241 cataaatt
//
LOCUS       DBMDXG010000081         6235 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3196_length_6235_cov_1.639061, whole genome shotgun sequence.
ACCESSION   DBMDXG010000081 DBMDXG010000000
VERSION     DBMDXG010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3196_length_6235_cov_1.639061"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="ACZPD3_03640"
     CDS             complement(<1..577)
                     /locus_tag="ACZPD3_03640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1415130.1"
                     /translation="MASGIDTVIFDMDGVIFDSEILVLQAWKEVAERHGIAGVEAACH
                     ECLGTNSVVSKGVFLKHYGEDFPYEEYKAEMAEVFFSHASGGKLAKKPGVEELLKYLK
                     MRGFKIGLASSTREVLVRSEISDGGLLGYFDQIVGGDMVERSKPEPDIFLEACRRLGT
                     RPENCYVIEDSHNGIRAAYAAGMHPIMVPDLM"
     gene            complement(692..>1960)
                     /gene="radA"
                     /locus_tag="ACZPD3_03645"
     CDS             complement(692..>1960)
                     /gene="radA"
                     /locus_tag="ACZPD3_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR1415131.1"
                     /translation="ETVSTKKPSSSGVMKSSEKRQEPVILKDISLSEDERQTTQIGEL
                     DRVLGGGIVPGSLVLVGGDPGIGKSTLLLQVCRNLAEKQVSVLYISGEESLRQIKLRA
                     NRIGQFTDKMQLLCETNLEVIREVIERRKPDVVVIDSIQTMFHEDVSSAPGSVSQVRE
                     STNILMQIAKGMGVSIFIVGHVTKEGNVAGPRVLEHMVDTVLYFEGDRHASYRILRAV
                     KNRFGSTNEIGVFEMCNTGLEEVKNPSEYMLNGRPEDASGSVVACSMEGTRPILVEIQ
                     ALVCQSNFGIPRRTAVGTDFNRVNLLMAVLEKKVGLRLAASDAYVNIAGGMKMTEPAI
                     DLGICLAIVSSAKDIVIPDNVMVFGEVGLSGEIRAVNMAGQRVQEAKKLGFGTVVLPE
                     VCRSSVGKVEGINLVYVSQIRDAIGYIMKK"
     assembly_gap    1961..1970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1971..2068)
                     /locus_tag="ACZPD3_03650"
     CDS             complement(<1971..2068)
                     /locus_tag="ACZPD3_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415132.1"
                     /translation="MLKNKKTVYFCQECGYESAKWMGQCPGCKSWNT"
     gene            complement(2062..2475)
                     /locus_tag="ACZPD3_03655"
     CDS             complement(2062..2475)
                     /locus_tag="ACZPD3_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HIR1415133.1"
                     /translation="MAVVKELIRTEENGAISFGDYELAQKSKLSDYQHQGDMYKVKTF
                     KEITKLERNGMFVYESVPGTAVFNLTQSEAQMDFHVEGPEDAQITVEMEPDTEYEVFI
                     EQASTGKMKTNLGGKLSFSVELGNAARVEVKIVKC"
     gene            complement(2705..5167)
                     /locus_tag="ACZPD3_03660"
     CDS             complement(2705..5167)
                     /locus_tag="ACZPD3_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR1415134.1"
                     /translation="MERQFTRQAENALKLATTIAKSCGHGYIGTEHLLAGLLKEPEGT
                     AGKVLQEFNVEEEKLRELISSLVTPVQGNTAAAETKDPQYSPRARRIIEQAEEDAESM
                     ECLCGTEHLLLSLLKETDCVATRLLFTMGVNIQKLFVAILTAMGFDNDTIAEEFQYAR
                     NAGNKKVTSTPTLDQYSRDLTQLAAEGKLDPVIGRDKEITRLIQILSRRTKNNPCLTG
                     EPGVGKTAIVEGLAQRIVMGMVPDTVKDKRLVVLDLSGMVAGSKYRGEFEERIKNVIQ
                     EVKEHQGILLFIDEIHTIIGAGGAEGALDASNILKPSLSRGEIQLIGATTLEEYRKYI
                     EKDAALERRFQPVTVEEPSSEETVEILKGLRPYYEKHHGVKIEDEALEAAVKMSQRYI
                     NDRFLPDKAIDIIDEAASKVQLAGYQSAPEMEQYELELNELREEKEQAVKTADIERAK
                     KAQVRQNEVEAEMEKIRIKTERRNKRKKLVVDEAAVAETISDWTKIPLQKLTEGETKR
                     LARLEKELHKRVIGQNEAVKAVAQAVKRGRVGLKDPNRPIGSFLFLGPTGVGKTELSK
                     ALAEAVFGSEQAMIRVDMSEYMEKHSVSKLIGSPPGYVGYEEGGQLSEKVRRNPYSVL
                     LFDEIEKAHPDVFNILLQVLDDGHITDAQGRKVDFKQTIIIMTSNAGAQAIMEPKRLG
                     FMSDNDEKKDYERMKGGVMEEVRRIFKPEFLNRIDDIMVFHVLNKEDIRKIVTLLLKT
                     LEKRCAEQMEIHLTVTNAVKDFIAEKGSDNKYGARPLRRAIQSKIEDALANEILEGRV
                     KRGDSVQVKLHKNEIAFEVKKQ"
     gene            complement(5176..5553)
                     /locus_tag="ACZPD3_03665"
     CDS             complement(5176..5553)
                     /locus_tag="ACZPD3_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR1415135.1"
                     /translation="MMIEIDFSSDEAIYIQLTNQIIMGIATSRLQEGDTLPSVRQLAD
                     TVGINMHTVNKAYSLLRQEGFVTIDRRRGAVISIDVNKRKALEELKQNLMVALAKGCC
                     KSVSREEVHQLIDEIFDEYDENR"
     gene            complement(5733..>6235)
                     /locus_tag="ACZPD3_03670"
     CDS             complement(5733..>6235)
                     /locus_tag="ACZPD3_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1415136.1"
                     /translation="VWVDGEDKIEIDLPDIAKQADMSKIEPAKRPLLNPTLELSGIYF
                     MQELRRPVEKDQHGNLRELEEELIVNLKKSEYLVAMNVDPEDPKKINIPYLKNKKEEI
                     LQPVFTDVMELEKFTKGQKLRIAKVPFAKLPELLIDKAMAYAVNPLGFNLVLNREQLN
                     KIIGLK"
BASE COUNT         1527 a         1714 c         1029 g         1955 t
ORIGIN      
        1 ccatcagatc cggcaccatg atcggatgca tccctgctgc ataagccgcc ctgattccat
       61 tatgagaatc ctcaatcaca taacaattct ccggtctggt ccccagtcgt ctgcaagcct
      121 ccagaaaaat atccggttcc ggcttactcc gttccaccat atctcccccg acaatctgat
      181 caaaatatcc cagcagcccg ccatctgaaa tctccgaacg caccagtacc tcccgggtag
      241 aactggcaag cccaatctta aatcccctca tcttcagata tttcaaaagc tcctcaaccc
      301 ccggcttctt tgccagtttg cctccggaag catgactgaa aaacacctct gccatctccg
      361 ctttatattc ctcatacgga aaatcttcac cataatgctt cagaaaaacc cctttactta
      421 caacactgtt tgttcccaga cactcatgac atgccgcctc aactcctgcg attccatgac
      481 gttctgccac ttctttccac gcttgaagca caagaatctc tgaatcaaaa atcactccat
      541 ccatatcaaa tatcactgta tcaatcccac tcgccataac ttttgatcct ttcctctttc
      601 tctctgtcag cagactttct caggcaaaaa tatctttgac gacagtctga tcgacttatt
      661 caaaccatat ataagccagt agtctgacaa tctatttttt cataatataa ccaatcgcat
      721 cacgaatctg cgacacataa accaagttga tcccttccac ttttccaaca gaagacctac
      781 acacttccgg aagcacaact gttccaaatc ccagtttttt cgcctcctgc acacgctggc
      841 ctgccatatt cactgcgcgg atttcaccac tcagaccaac ctcgccaaat accatcacat
      901 tatcaggaat cacaatatcc ttcgcactgg aaacaatcgc cagacagatc ccaaggtcaa
      961 ttgccggctc tgtcatcttc attccccctg caatattgac ataagcatcc gatgcagcca
     1021 gacggagtcc tactttcttc tcaagcacag ccataaggag attcactctg ttaaaatcag
     1081 ttcctaccgc tgtccgcctc ggaataccga aattactctg gcacacaagt gcctggattt
     1141 caaccagaat cggccgggtt ccttccatag aacacgccac aacagaaccc gacgcatcct
     1201 ccggtctgcc attcagcata tattccgacg gattcttcac ctcctcaaga cctgtattac
     1261 acatttcaaa aacaccaatc tcattcgtag atccaaaacg attcttcacc gcacgcagga
     1321 tacgataaga agcatgccgg tcgccttcaa aataaagcac cgtatccacc atatgttcca
     1381 gaaccctcgg acctgccaca tttccttctt tcgtcacatg acctacaata aaaatggaca
     1441 cccccattcc ctttgcgatc tgcataagga tattggtaga ttctctcacc tgagagacac
     1501 ttcccggtgc cgaacttaca tcttcgtgaa acatagtctg aatggagtcg atcacaacca
     1561 catccggttt tctgcgctcg atcacctcac gtatcacttc caggttcgtc tcacataaaa
     1621 gctgcatctt atccgtaaac tgcccgatac gattagctct cagcttaatt tgtctcagag
     1681 attcctcgcc tgaaatataa agaaccgaaa cctgcttctc tgccagattc ctgcacacct
     1741 gtaaaagcag cgttgatttt ccgattcccg gatctcctcc cactaaaaca agggagccgg
     1801 ggacgatacc gccgcccagc accctgtcaa gttcaccaat ctgagttgtc tgccgttcat
     1861 cttctgacag gctaatgtct ttaaggatca ccggttcctg ccttttttct gatgatttca
     1921 ttactccaga agaggatggc ttctttgtgg aaactgtctc nnnnnnnnnn gtgttccagg
     1981 acttgcatcc cgggcactgc cccatccatt ttgcggattc atatccgcac tcctggcaaa
     2041 aatataccgt ctttttattt ttcagcattt tacaatcttt acttctactc ttgcagcatt
     2101 gccaagttcc acgctaaagg aaagtttacc gcccaggttt gttttcatct tacctgtgct
     2161 tgcctgttca ataaatactt catattctgt atccggctcc atctccactg tgatctgtgc
     2221 atcttccggt ccctctacat ggaaatccat ctgagcttca ctctgtgtaa gattaaatac
     2281 tgctgttccc ggaactgatt cgtaaacgaa cattccgttt ctctcgagct tggtaatctc
     2341 cttaaaagtt tttactttat acatatcgcc ctgatgctga tagtctgata atttagactt
     2401 ctgtgcaagc tcatagtcac caaagctgat tgctccattc tcttctgtac ggattaattc
     2461 ttttactact gccatttcct atgtcctcct aagactcttt tgtttgccgc tccaccggtt
     2521 catctgccga tgccgtacag tttttcacta tctctgtact tttccgacat tttatgtgct
     2581 tattatataa tacattcact tttttgacca gtatctcact gattttttta tactaatttc
     2641 acaatttctg tgaatcatgt gagtcctctg ctttaattgt aaatatctgt ctgctcagag
     2701 atttctactg ttttttcact tcaaatgcaa tctcgttttt atgaagtttc acctgtacac
     2761 tatctccacg ttttactctg ccttccagaa tctcgtttgc aagcgcatct tctatcttac
     2821 tctgaattgc acgacgcagc ggtctggcac catatttatt atcagaacct ttttctgcaa
     2881 taaaatcttt cacagcattt gtcactgtaa gatgaatttc catctgttct gcgcatcgtt
     2941 tctccagtgt tttaagaagc aatgtcacaa ttttccggat atcttcttta ttcagcacat
     3001 ggaacaccat gatatcgtca attcggttca ggaactccgg tttgaaaatc cggcgcactt
     3061 cttccatcac accgcctttc attcgttcat agtctttctt ctcatcatta tcagacatga
     3121 atcccagacg tttcggttcc ataatcgcct gtgcccctgc gttggaagtc ataataataa
     3181 tcgtctgttt gaagtcgact tttcttccct gtgcatctgt aatatgaccg tcatccagca
     3241 cctgtaaaag tatattaaac acatccggat gtgccttctc aatctcatca aaaagcaata
     3301 cactgtacgg atttctgcgg accttctcac tgagctggcc gccttcttca tatcctacat
     3361 atcccggagg agaaccgatc agcttggaaa cactgtgttt ctccatatat tcggacatat
     3421 ctacacgaat catcgcctgt tcacttccaa agacagcttc tgccagagcc ttggaaagtt
     3481 ctgtcttacc aacacctgta ggtccaagga aaaggaaaga accaatggga cggttcggat
     3541 ctttaagtcc tactcttccc cgtttcactg cctgcgcaac agcttttact gcctcattct
     3601 gtccgatcac acgtttatga agttctttct ccagacgagc aagacgtttg gtttctccct
     3661 ctgtcagttt ctgaagagga atctttgtcc agtcagaaat cgtctctgca acagcagctt
     3721 catctacaac aagtttcttc ctcttatttc tacgttcagt cttaatgcgg attttctcca
     3781 tttcggcttc cacttcattt tggcgtacct gtgctttttt tgcgcgttca atatctgcag
     3841 tcttaactgc ctgttctttc tcctcacgaa gttcattcag ttccagttca tattgttcca
     3901 tttcaggtgc tgactgataa cccgcaagct gaacctttga tgctgcttca tcaataatat
     3961 caattgcctt atccggaagg aaacggtcat tgatatatct ttgtgacatt tttactgctg
     4021 cctcaagtgc ctcatcttca atctttaccc catgatgttt ctcataatat ggacgcagac
     4081 ctttaaggat ctcaacagtc tcctctgaag atggttcttc cactgtcaca ggctggaatc
     4141 ttcgttccag tgctgcgtct ttttcaatat atttacggta ttcctccaga gtagtcgcgc
     4201 cgataagctg aatctcacct ctggaaagag aaggtttcag aatattagaa gcatccagtg
     4261 ctccctccgc tccacctgct ccgatgatgg tatggatttc atcaatgaac agcagaattc
     4321 cctgatgttc ttttacttcc tggatcacat tcttaatgcg ttcctcaaat tcacctctgt
     4381 atttgcttcc cgcaaccatg ccggaaagat ctaacacaac aagacgctta tccttcactg
     4441 tatccggaac cattcccata acgatgcgct gtgcaagtcc ctctacaatg gcagtcttac
     4501 ctactccggg ttcccctgtc agacacggat tattcttcgt acgtctgctc agaatctgga
     4561 tcagtctggt aatttcttta tcacgcccta tcacaggatc cagttttccc tctgctgcaa
     4621 gctgtgtcag gtcacgactg tactgatcca gcgtaggggt actggtcact tttttgtttc
     4681 ccgcattcct tgcgtactga aattcctctg caatggtatc attgtcaaat cccatagctg
     4741 tcaggatagc cacaaacaac ttctggatat ttactcccat tgtaaaaagc agtctggtcg
     4801 ctacgcagtc tgtctctttc agaagcgaaa gcaacaggtg ctctgttcca caaagacatt
     4861 ccatactctc agcatcttcc tccgcctgtt caatgatcct tcttgctctc gggctgtact
     4921 ggggatcttt tgtttctgct gccgcagtat tgccctgaac cggtgtaacc aggctgctga
     4981 taagttccct gagtttttct tcttctacgt taaattcctg cagcactttt cccgcagtgc
     5041 cttcaggttc tttcaaaaga cctgcaagaa gatgctctgt tccgatatat ccatgtccac
     5101 aggatttcgc aattgtcgtt gccaatttga gggcattctc tgcctgtctg gtaaattgtc
     5161 tctccatgtc ctctcctatc tgttttcatc atattcgtca aaaatctcat caataagctg
     5221 atgtacttcc tctctgctta cgctcttgca acatcctttc gcaagcgcca ccatgagatt
     5281 ctgtttcagt tcttccaatg ctttccgttt atttacatca atagaaatca ctgcaccacg
     5341 tctgcggtcg atagtgacaa atccctcctg tcgcagcagt gaatatgctt tatttactgt
     5401 atgcatattt atcccaactg tatctgcaag ctgtctcact gatggcagtg tatctccctc
     5461 ctgcagtctt gacgtagcaa tccccatgat gatctgattg gtcagctgga tgtaaatggc
     5521 ttcatcactg ctgaaatcta tttctatcat catacgtttt cttctccttt ttgtgttaca
     5581 catatcctag cacaaaccaa cattttttgc aacagaaaga cgtattgctg tattattttt
     5641 caaaacaaaa aggggtgtac tgccatgttc tcacacagca tgcacaccct ttatcttctt
     5701 tctgtcaact gaatttcatc tacatgactt gattatttta agccaatgat cttattgagc
     5761 tgttctctgt tcagaacaag gttaaagcct aatggattaa ctgcataagc cattgcctta
     5821 tcgattaaca gctccggaag ttttgcaaac ggtactttcg caatacgcag cttctgacct
     5881 tttgtaaact tctcaagttc cataacgtct gtaaatacag gctggagaat ttcttctttt
     5941 ttatttttga gatacggaat attgatcttc ttaggatctt caggatctac attcatagca
     6001 accagatatt cggatttctt caggtttacg atcagttctt cctcaagctc acgaagattt
     6061 ccatgctgat ccttctctac cggacggcgg agttcctgca taaagtaaat gccggaaagc
     6121 tccagtgtag gattcagaag cggtctctta gccggctcaa tcttgctcat atctgcctgt
     6181 tttgcaatat caggaagatc aatctcaatc ttatcttcac catctaccca tacaa
//
LOCUS       DBMDXG010000082         6187 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3228_length_6187_cov_1.866579, whole genome shotgun sequence.
ACCESSION   DBMDXG010000082 DBMDXG010000000
VERSION     DBMDXG010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_6187_cov_1.866579"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            114..830
                     /locus_tag="ACZPD3_03675"
     CDS             114..830
                     /locus_tag="ACZPD3_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1415137.1"
                     /translation="MRIAIVDDISEERTLLRNRLESQFSRRNVHTDILEYENGEAFLT
                     AAKECPFTVVFLDIYMNGSNGIDTAKELRRSDTDCLLVFTTTSTDHALEGFQVRALHY
                     LVKPYSENDISALADEILSRIPDSGKYIDVKVNGSNIQIPFRKIIYAEHFSHMIYIHT
                     AGERELVTRQSFDSFITSLKMDPRFYQCNRGVVINLEHAVDFDGTGFCLDNGSNIPVS
                     RKLLKNARQTFMEFLFQRRF"
     gene            832..2049
                     /locus_tag="ACZPD3_03680"
     CDS             832..2049
                     /locus_tag="ACZPD3_03680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415138.1"
                     /translation="MADILRPVLELSVIIPGILLAYLPVKSYLKQTPFKLTAWLLPLL
                     LGICIFGGAVCCAFHFPTRWVLFPLLPVIMLIYHKTLKISVWKSVSIFLAVFAVFACI
                     NSLSRAVNTLITADLHITENELWFHTGAGIFYNVICLLFVLAAWYPAHHRVQTMIADE
                     NFAQTWYVFWILPVIFIGVNLFMIPKYRSTLYTGRVLQCYIVLSLVLLIILLLFYAMF
                     LMLAVSLNRNARLQHENHLLSLQQERYKNLCMAIEEARQARHDIRHHFVQLSALAEQG
                     DMEKIKKYLLAATGKISDYNLHFCENQAVDSVFGYYSALAKRENIPFHALVSLPADLS
                     IDEIDLCLVFSNLLENAIQASVKTEPARRKINVEVYPHHNHLLLIHVENTFNGKIQQK
                     TIFSNPPNTPETA"
     gene            2052..2150
                     /locus_tag="ACZPD3_03685"
     CDS             2052..2150
                     /locus_tag="ACZPD3_03685"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415139.1"
                     /translation="MESVRHITDKNGGACDFTYEDGIFSAKIMLRI"
     gene            complement(2314..2526)
                     /locus_tag="ACZPD3_03690"
     CDS             complement(2314..2526)
                     /locus_tag="ACZPD3_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415140.1"
                     /translation="MADFIKKAIELEAIDSAGDLYGDDDDLSRDEYIERDKGVLLYMK
                     KHPEIRKIVEEQIGEPITSIWKDAEV"
     gene            complement(2729..3058)
                     /locus_tag="ACZPD3_03695"
     CDS             complement(2729..3058)
                     /locus_tag="ACZPD3_03695"
                     /inference="COORDINATES: protein motif:HMM:NF046294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase domain-containing protein"
                     /protein_id="HIR1415141.1"
                     /translation="MNGFEKYDKPRMNQLPGESYTLCDYKYFLKVPDNYHLEYDAHYY
                     SVLYTYKGKLAILKATMTEIRICDEYNRLICRHPRYPHKPQTALRYAPRCSCGLCGLF
                     CYMINKL"
     gene            complement(3075..3281)
                     /locus_tag="ACZPD3_03700"
     CDS             complement(3075..3281)
                     /locus_tag="ACZPD3_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415142.1"
                     /translation="MASRIQGTTVEIGGDTTKLSKALESVNKSIKGTQSGLKERHLYL
                     SGSPECCGQKDRSGHQPASFPEKI"
     gene            complement(3397..4794)
                     /locus_tag="ACZPD3_03705"
     CDS             complement(3397..4794)
                     /locus_tag="ACZPD3_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1415143.1"
                     /translation="MSQFYCREDELRKLNKRYAGDKFECIVIYGRRRVGKTALINEFC
                     KDKPTIFFSALNTTGKENLEALSKSIMSFERPDMESAPEFRSYDAALDELTALSKEKR
                     IVFVIDEYPYLAKAKPAISAMLQHIIDHKWTESKMYLILCGSSMSFMESQVLGKESPL
                     YGRRTGQFKIEPLDYKETAVFHPNLSAEDNSLIYGITGGVPHYINKLDVRDSVDEALL
                     DNFFDRSSYLYEEPGNLLKQELREPAIYNAIIKAIAEGASRMNDIKMKVGEENSVVSK
                     YLKTLIDLGIAKKETPITEKPGKKTIYLLADNFFRFWYRFVPINMSAIDSGRIAKTYP
                     HAVKQYLPDYMGLIYEKMCQDYLLYYSDSLPIELSEIGQWWGTDPKKKKQIQIDIVGT
                     PVEGKDYIIGSCKYRNEKIGVDELDLIRDYASVFGKGNNYHYYIFSKGGFTDGLLQAQ
                     ERGEVRLITLEDLYK"
     gene            complement(5166..5708)
                     /locus_tag="ACZPD3_03710"
     CDS             complement(5166..5708)
                     /locus_tag="ACZPD3_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415144.1"
                     /translation="MYIFIPFFFFIWGGLTVAYFHFGFQPEWLATILNTSSAGDSAAN
                     AFMLSTRGGLFIIGVGVFCLIWIVVRLWLGIDKFEEPQDSGSLREDIVEIFDALLTPI
                     PSYEEEKETDPLSFGALFDELSSTSSSEPEYLSDSDGKSVRITGRGSDVFYLENGDVV
                     YTTDGRDFYTTDDDVYHHYE"
BASE COUNT         1684 a         1474 c         1234 g         1795 t
ORIGIN      
        1 tctgcgaatt cgattgcaca gatgaaaaaa tattaccgaa aacagcgtga acagtaacga
       61 ggaattctca gcaggcagaa ttttataaat atatattata aggaagattt actatgagaa
      121 ttgcaattgt tgatgatatt tcagaagaac ggacactgtt acgaaacaga ctggaatctc
      181 agttttccag gcgcaatgtc catacagata tacttgaata tgaaaatgga gaagccttcc
      241 tcactgcggc aaaggaatgt cctttcactg tagtgtttct ggatatttat atgaatggtt
      301 ccaacgggat cgatacagca aaagaacttc gcagatcaga cactgactgt ctgctggtct
      361 tcacaacaac ttccactgac catgcattag aaggatttca ggtccgggct ctgcactatc
      421 ttgtaaagcc ctacagtgaa aatgatattt ccgcactggc agatgaaatc ctttcccgca
      481 tcccggattc cgggaaatac atagatgtaa aggtcaacgg cagcaatatt cagatccctt
      541 tccggaaaat aatctacgca gaacattttt cccatatgat ctacatccac actgccggtg
      601 aaagggaact ggtcacccgc cagtcctttg attccttcat aacctccctg aaaatggatc
      661 cacgcttcta tcagtgtaac cgaggcgtcg tgatcaatct ggaacatgca gttgattttg
      721 acggaacagg cttttgcctt gataacggaa gcaatatccc ggtaagcaga aagcttctca
      781 aaaacgccag acagacattt atggaatttc tgttccaaag gaggttctga aatggctgac
      841 atacttcgcc ctgtacttga gctttccgta atcattcccg gtatactgct ggcatatctg
      901 cctgtaaaaa gctatctgaa gcagacaccg tttaaactga cagcgtggct tcttcctctt
      961 cttctgggaa tctgtatttt cggaggagcg gtttgctgtg ccttccactt tcccaccaga
     1021 tgggtcctgt ttcctctgct gcccgttatt atgcttattt atcataaaac gctaaaaatc
     1081 tctgtctgga aatccgttag tatttttctg gcagtctttg cagtttttgc atgtatcaac
     1141 agcctgtccc gggcagtcaa tacacttata actgctgacc tgcatattac agaaaatgaa
     1201 ctgtggtttc ataccggtgc cggaattttc tataatgtca tctgcctgct gtttgttctg
     1261 gcagcctggt atcctgccca ccaccgtgta cagacaatga tcgcagacga aaactttgcc
     1321 cagacctggt atgtattctg gattcttccg gtaatcttta tcggagtgaa tctttttatg
     1381 atccctaaat accgcagtac tttatatacc ggacgggtcc tgcagtgtta tattgtttta
     1441 agtcttgtac tgctcataat cctgctcctg ttttatgcca tgtttctcat gctggcggtc
     1501 agcctgaaca gaaatgccag acttcagcat gagaaccatc ttctttccct tcagcaggaa
     1561 aggtacaaga atctctgcat ggccattgaa gaagcaagac aggcccgtca cgatatccgc
     1621 catcattttg tccagctgtc cgctctggca gaacagggtg acatggaaaa gatcaaaaaa
     1681 tatctcttgg ctgcaaccgg caaaatctct gactataacc tgcatttctg tgaaaatcag
     1741 gctgttgaca gtgttttcgg atattattcc gcccttgcca aaagagaaaa catccctttt
     1801 catgcactgg tttccctgcc tgctgatctt tccatagacg agattgatct gtgtcttgtg
     1861 ttttccaatc tgctggaaaa tgccatccag gcaagtgtaa aaacagaacc cgcccgcaga
     1921 aagatcaatg tggaggttta tccccaccat aatcatctgc ttctgattca tgtggagaat
     1981 accttcaatg gcaaaataca gcaaaaaaca atattttcca atcctccaaa cactccggaa
     2041 acggcatagg gatcgaatct gtccgccaca ttaccgacaa aaacggcggt gcctgtgact
     2101 ttacttatga agatggtatt ttctctgcca aaataatgct ccggatatga tcagttatcc
     2161 gggcagcacc tgcaattgcc cgttcttcac ataggcaaca gtcttgcggc catttacaac
     2221 tgcacgggtt gcaaggagca cccgctttcc ttgcgacgta taaattccac attttgaaat
     2281 caatgttaaa tgtgcctctt tacttttggc aaattacacc tcagcatctt tccaaatcga
     2341 tgtaatcggt tctccaattt gttcctcaac gattttccgt atttctgggt gctttttcat
     2401 gtaaagaagc actcccttat ctctctcaat atattcatcc cggctaagat catcgtcatc
     2461 accatacaaa tctccggcac tatcaatcgc ttcgagttct attgcctttt taataaagtc
     2521 agccaagtca ctgcaacaaa tagctgctta ccaacgcact aaatgtctca gcagatgatc
     2581 ttcttgtaga cagtttgacg cactccgcgt ccactgccga ttccgagatc caccgtctgc
     2641 ttttagactg taatgcaatt gagcaggaga ttctcacccg gatggtaaag gagatgaaag
     2701 caattttata cggcttagga gtttgatttt ataacttgtt gatcatataa caaaaaagcc
     2761 cgcataagcc acaactgcac cttggagcat accgaagtgc tgtctgtggt ttatgcgggt
     2821 atctcggatg tcggcagatc agccggttat actcgtcgca gatccgtatc tccgtcatgg
     2881 tagccttaag aattgcaagc ttccccttat aggtatacaa tacagaatag tagtgggcat
     2941 catactcaag gtggtaatta tctggaactt taagaaagta tttataatca caaagtgtat
     3001 aactttcgcc tggaagctgg ttcatacgcg gtttgtcgta cttttcaaat ccattcatgc
     3061 gggatttccg aatgtcagat tttttctgga aaggacgccg gttgatgtcc gctacgatct
     3121 ttttgaccgc agcattcagg gcttccagag aggtataagt gtctttcttt cagtccggac
     3181 tgcgtcccct tgattgactt gtttacactt tccagtgctt tggagagctt tgtggtatcg
     3241 ccgccgatct caacggtggt gccctggatt ctggatgcca tttgcgtaac cacctccttg
     3301 ccggcatgaa aaaagcccat ctgcacggag cagacaggct aaaaatgggt ataaaaatac
     3361 cctgccagct ttcaccagca gggttgatta gaatttttac ttgtaaaggt cttccagcgt
     3421 tatcagccga acttcacctc gctcttgtgc ctgaagaagt ccatctgtga atccgccttt
     3481 cgagaagata tagtagtgat agttgttgcc ctttccaaaa actgaggcat aatcccgaat
     3541 caaatcgagt tcatccacac cgattttctc attccggtat ttgcatgaac cgatgatata
     3601 gtctttgccc tcaacaggag ttccgacgat atcaatctgt atctgctttt tcttcttcgg
     3661 gtctgtcccc caccactggc cgatttcact cagctcaata ggaaggctat ccgaataata
     3721 gagcaggtaa tcctgacaca tcttctcata aattagaccc atataatcag gaagatactg
     3781 ctttacagcg tgtggatagg tctttgcaat tctgccagag tcaatggcac tcatattgat
     3841 tggcacaaac cgataccaga aacggaagaa gttatcagcc agcagataga tggttttctt
     3901 acccggtttt tctgtaatcg gtgtttcttt cttcgcaatg ccaaggtcga tcagcgtttt
     3961 caggtacttc gatacgaccg agttttcctc gccgactttc atcttgatat cgttcattcg
     4021 ggaagcacct tctgcaatcg ctttaatgat tgcattataa atggccggct cccggagttc
     4081 ctgcttcagt aagttccccg gttcctcata cagatagctg gagcggtcaa agaaattatc
     4141 caacagagct tcatccacac tatctcgcac atccaactta ttgatatagt ggggaactcc
     4201 tcccgtgatc ccataaatca gggaattgtc ttctgcagac agatttgggt ggaacacagc
     4261 ggtttcttta tagtctagcg gctcaatctt aaactggcca gtacgtctgc catacaacgg
     4321 actttccttt ccaagcacct gactctccat gaagctcata gaggagccgc aaagaatcag
     4381 gtacatcttg gactctgtcc atttgtggtc gatgatgtgc tgcagcatcg ctgaaatagc
     4441 tggttttgct tttgcaagat aaggatactc atcaatgaca aagacaatcc tcttttcctt
     4501 tgaaagtgct gtcagctcat ccaaagctgc atcataggac ctgaactccg gtgcagactc
     4561 catatccggc cgctcaaaac tcataatcga ctttgagaga gcttccagat tttcctttcc
     4621 tgtcgtattc aatgcagaaa agaaaatggt aggcttatct ttgcagaact cgttgatcag
     4681 tgccgtttta ccaacacgcc gtctgccgta gatgacaatg cactcaaact tatcacctgc
     4741 ataccgttta ttcagtttcc gcagctcatc ctcacggcaa tagaattgac tcatctgctc
     4801 acctccaata attatactcg tgagttagta actcgtaagt tacttcttta cgagtataat
     4861 aaatcagata gtctgcaaag tcaagtaaaa taacgataag tacatatgtt gccttccatc
     4921 ctgaccacga tgtcggccct ctggttcttt tgggatttcg gcgctcacta gctatccctg
     4981 ttaccttgct gtctacgctt agcctgcagc gttgccgcgg cagactcaag actcgcttca
     5041 aatgctgtgg ttagcagctt atttgatggg gcttccaccc accggactat ataccctttg
     5101 ctggacgcac gggcagaccc caatgtggca aacatactgc tccactgctt tcaaatacgc
     5161 ccgcttcatt catagtgatg atacacatca tcatctgtcg tatagaaatc acgaccgtct
     5221 gtagtataga caacatcacc gttttccaga taaaaaacat cagaaccgcg gccggtgata
     5281 cgaacgcttt tgccatcact gtcactcaga tattccggct ccgaggaaga tgtactggaa
     5341 agttcatcga agagtgcacc aaagctaagt ggatctgttt ctttctcctc ttcatacgag
     5401 ggaatgggtg tgagcaaagc gtcaaaaatc tccacaatat cttcgcgcaa agaaccagag
     5461 tcttgaggtt cttcgaattt atcaatccca agccaaagac gaactacaat ccagatcagg
     5521 caaaaaacac cgacaccgat gataaaaagt ccaccgcgtg tgctgagcat aaaagcattg
     5581 gctgcgctgt cgccggcgga actcgtattc agaatcgtag caagccattc tggctgaaat
     5641 ccgaaatgaa aataagccac cgtcagaccg ccccagatga aaaagaaaaa tggtatgaat
     5701 atgtacatta taatcgcctc ctgtctcaat acagaaaaaa cagctgcaac acagcaatgg
     5761 caggatcagg aagcgctccg ccgctttcaa ctgatttcgc cgctctgaca tacctgaaaa
     5821 ttaaagcaaa cttgttactg ttcctttata tgaactccgc actaattttt gtacttcgtt
     5881 taattgcatt actttcatcc gctctaattc gcattcgtca atagtatcag gggtaaaaat
     5941 cctgattttt acatttccag tgtccgtatt gttcttaatt gtgatatcat ataattttat
     6001 ttttttacaa acgatttttc tttcataatt tctccgaaaa taattttata caaagatgat
     6061 ttatttgctg acttgtgata tacttaaagt aatgtgtgga ctggggaatt tagtataatg
     6121 tacgtttgta ttctaataaa gtatgaaata taaagagaag atctgtatgc gttaataacg
     6181 cacgcag
//
LOCUS       DBMDXG010000083         6154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3252_length_6154_cov_1.363621, whole genome shotgun sequence.
ACCESSION   DBMDXG010000083 DBMDXG010000000
VERSION     DBMDXG010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3252_length_6154_cov_1.363621"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            59..868
                     /locus_tag="ACZPD3_03715"
     CDS             59..868
                     /locus_tag="ACZPD3_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012471.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR1415145.1"
                     /translation="MIGRSHDDRMIPMLEIGKGDTCIICLSGVESGDRNLPEYLLSIA
                     KDYCRSYESNWTIGESYEVRKLLDKVRICMIPMLNPDSYEICEYGYGAIHNPIHRQML
                     KMQDRPVEEYECNARGIDLRRNFPTNYYQRKRVNQEPASENETRALISIFQEYSSLGL
                     LTFSYSRGKIVYCRQEKGFAYNQKNYRLARHLQKCSGYRLEKGIAGGARVKKAGAKPE
                     MGSPEQFYAEVIRQPSLAIEIPEYRKDDMEELRLIPLEYLYSLNSGILANA"
     gene            1022..2422
                     /gene="miaB"
                     /locus_tag="ACZPD3_03720"
     CDS             1022..2422
                     /gene="miaB"
                     /locus_tag="ACZPD3_03720"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR1415146.1"
                     /translation="MNNNTQESKIQLEYINKCTELVKEKYKKAPAFFIQNAGCQMNSL
                     QTDTVAGIVKRMGYTEVSREEDADVVIYNTCTVRENANLKIYGHLGHLKSIKKKNPEL
                     KIVLFGCMMQEPEVIEKIHKEYSFVDLVFGTHNFHKFPELFYRSLNTEGQIIDVWKES
                     DEIVEGMPSDRKYSFKTGVNIMFGCNNFCSYCIVPYVRGREKSREPEAIIEEIKGLVA
                     DGVTEVMLLGQNVNSYGKTLEHPVTFAQLLKQVEAIEGLKRIRFMTSHPKDLSDELIR
                     TMAESKKVCHHLHLPMQSGSSRILKIMNRRYDKEKYLELVAKIRNAVPDISLTTDIIV
                     GFPGETEEDFQDTLDVVEKCDFDSAFTFIYSKRSGTPAAKMENQVPEDVVKDRFDRLL
                     ALVQEKGRKASSRFEGTVQEILVEEESREKGIFTGRTEYNLLVHFPGCQDLIGKYVKV
                     KLDTCKGFYYFGSLAE"
     gene            2534..2981
                     /locus_tag="ACZPD3_03725"
                     /pseudo
     CDS             2534..2981
                     /locus_tag="ACZPD3_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     assembly_gap    2982..2991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2992..3890
                     /locus_tag="ACZPD3_03730"
     CDS             <2992..3890
                     /locus_tag="ACZPD3_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415147.1"
                     /translation="HAYKPPRFNLRAVGDTKRPLIFLVISGVVNAVLNLILVIMFHMD
                     VAGVAIATVISQLISCILVLRCLRTSKTSYQLHFGKLRINTVYLKQIFQVGIPAGIQS
                     TVINLSNALLQSSVNSFGSTAMAGYTAANNIFGFLYVAVNSVTQACMSFTSQNYGVHK
                     FKRMDKVLVDCLIISVVTSFSLGCGAYFFGSEILKIYTADPEVIRCGLEILSYTTVPY
                     FLCGIMDLFPGALRGMGHSGVPMILSVIGTVGTRIVWIFGIFPHHRSLAVLFISYPAS
                     WMLTIIMQVTCFYFVRRKVHRV"
     gene            4332..4649
                     /locus_tag="ACZPD3_03735"
     CDS             4332..4649
                     /locus_tag="ACZPD3_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1415148.1"
                     /translation="MADTQEKKVCPCCTKHTMRSEEERKKLINRLKRVEGQIRGIIGM
                     LENDAYCNDILIQSAAVNAAVNAFNKELLASHIRNCVARDIREGKDETIDELVATLQK
                     LMK"
     gene            4768..>6154
                     /locus_tag="ACZPD3_03740"
     CDS             4768..>6154
                     /locus_tag="ACZPD3_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1415149.1"
                     /translation="MEQYNVTGMSCAACSSRVEKAVSRVPGVTSCSVSLLTNSMGVEG
                     TAGSGEIIKAVQDAGYGASLKGASGEQISASAAEEALEDHETPALKRRLIASVGFLLV
                     LMYFSMGHMMWAWPLPAWFNDNHIAMGLVQLLLAGIIMVINQKFFVSGFKSLWHRALN
                     MDTLVALGSMASFLWSVYVLFAMTRAQVDGDSAAVMNYMMEFYFESAAMILTLITVGK
                     MLEARSKGKTTDALKGLMKLAPKTAVVVRDGQEATVPIEQVRKGDVFVVRPGENIPVD
                     GVVLEGNSAVNEAALTGESIPVDKNPGDAVSAATVNQSGFIRCEATRVGEDTTLSQII
                     KMVSDAAATKAPIAKIADRVSGVFVPAVISIAVITTIVWLLTGKEFGYALARGISVLV
                     ISCPCALGLATPVAIMVGNGMGAKNGILFKTAVSLEEAGNRPSPHAIGKSPSVCCMHR
                     FFHSSMQPRQYP"
BASE COUNT         1772 a         1209 c         1529 g         1634 t
ORIGIN      
        1 cgtatgggca ggtggaaaaa ccctttaagg acagagtgca ttttttgtcc agttcagagt
       61 gatcggaaga agtcatgatg acagaatgat ccccatgctt gagattggaa agggtgatac
      121 ctgcattatc tgtctgagcg gagtggaaag cggagacaga aatctcccgg aatatctttt
      181 gtccattgca aaagattact gcagatccta tgaaagcaac tggaccatcg gagaaagtta
      241 tgaagttcgt aaacttctgg ataaggttcg tatctgtatg atccccatgc tgaatcctga
      301 cagctatgag atatgtgaat acggatatgg tgccatacat aatcccatcc acagacagat
      361 gctgaaaatg caggacaggc ctgtggaaga gtatgaatgt aatgccaggg gaattgatct
      421 gcgcagaaat tttccgacta attattatca gcggaaaaga gtgaatcagg agcctgccag
      481 tgagaatgag actcgtgccc tgatcagcat ttttcaggaa tattcaagcc ttggcctgct
      541 tacattcagt tattccagag ggaagatcgt ttactgcaga caggagaaag gctttgccta
      601 caatcagaaa aattacagac tggcaaggca tttacagaaa tgttccggct atcgtctgga
      661 aaaagggatt gcaggtggtg cgagagtcaa aaaagccgga gcaaaacctg agatgggatc
      721 tccggaacag ttttatgcgg aagtaatccg tcagccctct ctggcaattg agattccgga
      781 ataccgcaag gacgatatgg aagaactgcg tctgattcca cttgaatatc tttattcttt
      841 aaacagcggc atattggcaa acgcataaat ccgtcacaca gtcaaagaaa aacagtctct
      901 gaaaataaaa agtaataaaa acatgtaaaa acagagatta gcgtttgaca taaagccata
      961 gattctgtat gataaaaaga gtgccccgaa atgttgtaaa cagaaagatg aggaatcaat
     1021 agtgaataat aatacacagg aaagtaaaat acagttagaa tatatcaata aatgtacaga
     1081 gcttgtgaag gaaaaatata aaaaggcgcc agctttcttt atccagaatg caggctgcca
     1141 gatgaattcc ctgcagacgg atacagtggc cggtatcgtg aaacgtatgg gctatacaga
     1201 agtatccaga gaagaagatg cagatgttgt catctataac acctgcacag tccgggagaa
     1261 tgcaaacctg aagatttacg gtcatcttgg ccatctgaaa agtattaaga aaaagaatcc
     1321 tgaactgaag attgtattgt ttggctgcat gatgcaggag ccggaagtaa ttgagaagat
     1381 ccacaaagaa tattcttttg ttgatctggt tttcggtacc cataacttcc ataagtttcc
     1441 ggagcttttc tacagaagct taaatacgga gggacagatc attgatgtct ggaaggaatc
     1501 tgatgaaatc gtggagggta tgccttctga ccgtaaatat tcctttaaaa cgggtgtcaa
     1561 tattatgttt ggctgcaata atttctgcag ttactgtatt gtaccatatg taagaggaag
     1621 agagaagagt cgcgaaccgg aggcgatcat agaagagatc aagggtctgg tggctgacgg
     1681 tgtaacggaa gttatgcttc tgggacagaa cgttaattcc tatggaaaga cactggaaca
     1741 tccggttact tttgcacagc ttctgaaaca ggtggaagca attgaaggat taaagagaat
     1801 ccgtttcatg acttcccatc cgaaggatct gtctgatgaa ctgatccgga caatggcaga
     1861 gagcaaaaaa gtgtgccatc acctccacct tcccatgcag tccggaagca gccgaatctt
     1921 aaagatcatg aaccgccgtt atgacaaaga aaagtatctg gagcttgtag ccaagatccg
     1981 taatgccgta ccggatattt cccttactac agatatcatt gtaggttttc cgggagaaac
     2041 ggaagaggat ttccaggata ctctggacgt tgtggaaaaa tgcgattttg attctgcgtt
     2101 tacctttatt tattccaaaa gaagcggcac acctgctgcc aaaatggaga atcaggtacc
     2161 tgaggatgtg gtaaaagacc gttttgaccg tcttcttgct ctggttcagg aaaaaggccg
     2221 aaaggcttct tcccgtttcg agggaactgt tcaggaaatc cttgtagagg aagaaagcag
     2281 agaaaaaggt atctttacag gaagaacaga atataacctt ctggtccatt tcccgggatg
     2341 tcaggatctg atcggtaaat atgtaaaagt aaaactggat acctgcaagg gattctatta
     2401 tttcggaagc cttgcagaat aaaaatgatg gctgtttctg aacagcaata taaaggactt
     2461 ctacagaagc ccatatcaat ttggatatag agggtggaag ataaaagtga aacaaaataa
     2521 atacgaaata gatatgtgta acggaaccat catggacaag ctgatttcct tttcactgcc
     2581 gctgatgctg tccggaatcc tgcagctgat gtttaatgct gtggatatca tagtggtggg
     2641 aagatttagc ggaagtcagg cacttgcagc cgtaggctca accacagcac tgatcaatgt
     2701 gtttaccaat ctgtttattg gtatctcact tggtgcgaat gtacttgctg cccggttcta
     2761 tgcggcagga aaagaccggg aaatgtcaga taccgttcat acggctgtaa ctcttgcact
     2821 ggtaagcggt atcgtcatgg catttgtggg actgattttt tccagatggg ctctggaact
     2881 gatgggtacc ccggacgatg tgatcggaca gtctgcactg tatatgaaaa tctattttct
     2941 gggtatgtat ggatgctgcc cggcatgggc gggatgccgg tnnnnnnnnn ngccatgctt
     3001 acaaacctcc taggtttaat ctgcgggctg taggagatac gaaacgtcca ctgatctttc
     3061 tggttatttc cggtgttgta aatgcagtac tgaatctgat cctcgtgatc atgtttcata
     3121 tggatgtggc gggcgtagcc attgctactg tgatctccca gttaatttcc tgcattctgg
     3181 tactcagatg tctgcgtaca tcgaaaacca gctaccagct tcattttgga aaattaagga
     3241 tcaatactgt ttacttaaaa cagattttcc aggttgggat tccggccggg atccagagca
     3301 cagtcattaa tctgtcaaat gctctgcttc agtcctcggt taactccttt ggatcgaccg
     3361 ccatggcagg gtatacggcg gccaataata ttttcggttt tctgtatgtg gcggtgaact
     3421 ctgtcacaca ggcatgtatg agctttacca gtcagaacta tggtgtgcat aaatttaaac
     3481 gtatggataa ggttctggtc gactgtctta ttatttccgt agttacctcc ttttcccttg
     3541 gatgcggtgc atatttcttc ggttctgaga ttcttaagat ttatactgcc gatccggaag
     3601 tgatccgctg cggactggaa atcctgtctt acactaccgt accttatttt ctctgcggga
     3661 ttatggatct gtttccgggt gccctgcgtg gaatgggaca ttccggcgtt ccaatgatcc
     3721 tgtcagtgat cggaacagta ggaaccagga tcgtatggat atttggaata ttcccgcatc
     3781 acagatcact ggctgttctg tttatttcct atcctgcatc ctggatgctg acgatcatta
     3841 tgcaggtcac ctgtttttat tttgtcagaa gaaaagtgca tagagtatag gtctttgagc
     3901 ttttgcgaaa aataattacc ggcagaatat taccggcggt tattttctgt gagagctctt
     3961 ttttgtgtta ctgctgctta ctctgtccac agtaactaat ctttttgttt ccgatatgaa
     4021 aaaacagtat tgacaaatac cccataaggg tatatagtga ctactgtaaa gataccccgt
     4081 aagggtattt tgaaatcgtt atatgtttca gaaatttata taaaacagag cttatttata
     4141 aaaatgtact ctcggagtct ttattgcact tgcttttatg gctgattttt cgccagatgc
     4201 acccgcattt tatcaacgta cgcaccgcgt acgcctcaaa aattcgggca catctgacaa
     4261 aaaatcttct cataaaatca agtacaaaaa gattccgaga gtacataaaa atcagaagga
     4321 ggatttccca tatggcagat acacaggaga aaaaagtctg tccctgctgc acaaaacaca
     4381 caatgcgttc tgaggaggag agaaagaagc tgatcaatcg tcttaaacgt gtggaaggac
     4441 agatccgagg gattatcggt atgctggaga atgatgcgta ctgtaacgat atcctgatcc
     4501 agtccgcagc agtcaacgca gcagtgaacg catttaacaa agaactgctt gccagccata
     4561 tccgcaactg tgtggcaaga gatatccgcg agggcaagga tgagacgatt gatgagctgg
     4621 tggctacttt gcagaaactg atgaagtgaa ggctgtgtgt tcagactatt atagcatcag
     4681 agtacagcag aataaagatc tgggaactgc aaagcaacag agcagtagat atcaggtaag
     4741 gaacaacaag tcgtaggagg aatagaaatg gaacaataca atgtaaccgg aatgagctgt
     4801 gcggcctgca gcagcagagt ggagaaggcc gtatccaggg ttccgggtgt gacatcctgt
     4861 tcggtcagtc tgctgaccaa ctccatggga gtagagggaa ctgccggttc aggtgagatc
     4921 atcaaggctg tacaggatgc aggatacgga gcatccttaa agggggcatc cggggagcag
     4981 atatctgctt ctgcggcaga ggaggcactg gaagaccatg aaacaccggc gctgaaacga
     5041 aggctgatcg catctgtagg ctttctgctt gtgctgatgt atttttccat gggacatatg
     5101 atgtgggcct ggcctcttcc ggcatggttt aatgataatc atattgccat gggactggtg
     5161 cagcttcttc tcgccggaat cataatggtc attaaccaga aattttttgt cagcggattt
     5221 aagagcctgt ggcatcgtgc actaaatatg gatacccttg tggcacttgg ctccatggca
     5281 tcctttttgt ggagtgttta tgtacttttt gcaatgacaa gggcacaggt agacggagat
     5341 tcggcggcag ttatgaatta catgatggag ttctattttg aatctgcagc catgatcctt
     5401 accctgatca cagttggaaa aatgctggaa gcccgatcta agggaaagac aacggatgcg
     5461 ctgaaaggtc tgatgaagct ggcacctaag acagcagttg ttgtccggga cggacaggaa
     5521 gctacagtgc ccatcgaaca ggtccgaaaa ggtgatgttt ttgtggtacg tcccggtgag
     5581 aacattccgg tggatggagt ggtccttgag ggaaacagtg ctgtcaatga ggcggcactt
     5641 acaggggaaa gtattcctgt tgacaagaat ccgggagatg cagtctctgc tgcaaccgta
     5701 aaccagtctg gatttatcag atgcgaagct actagggtag gcgaggacac tactctttcc
     5761 cagattatta agatggtaag tgatgcggct gcaacaaaag ctcctattgc aaaaatcgca
     5821 gacagggttt ccggagtttt tgttccggca gttatcagta ttgcggttat caccaccatt
     5881 gtctggctgc tgacaggaaa agagttcgga tatgcacttg ccagaggtat ttctgttctg
     5941 gtcatcagct gtccctgtgc ccttgggctt gccacaccgg ttgccattat ggtaggaaac
     6001 ggaatggggg ccaaaaacgg aatcctgttt aaaacagcag tatccctgga ggaagcaggt
     6061 aatcggccaa gtccacacgc aattggaaag agtccgtcag tttgttgtat gcatagattt
     6121 ttccattctt caatgcaacc cagacaatac cctc
//
LOCUS       DBMDXG010000084         5972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3361_length_5972_cov_2.060807, whole genome shotgun sequence.
ACCESSION   DBMDXG010000084 DBMDXG010000000
VERSION     DBMDXG010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3361_length_5972_cov_2.060807"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            195..851
                     /locus_tag="ACZPD3_03745"
     CDS             195..851
                     /locus_tag="ACZPD3_03745"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1415150.1"
                     /translation="MNYDSVPLRDFKELNDWETVIFVYQSALKQVETKIDILNGEFQH
                     RHKYNPIEHVKSRIKTPESIVKKLKRHGYESSIENMVRYVNDIAGIRISCSFTSDIYL
                     IADMISKQNDLTILARRDYMKNPKKSGYRSYHMLVTTPVFLSDSIIDTKVEIQIRTVA
                     QDFWATLEHKMHYKFEGDGPDYITKELRECARYVAELDTRMEELNNEIQKYGKLSDKK
                     "
     gene            complement(1185..1637)
                     /locus_tag="ACZPD3_03750"
     CDS             complement(1185..1637)
                     /locus_tag="ACZPD3_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HIR1415151.1"
                     /translation="MRIKKAGILIIAAAITCTTPAVPVMAADTSESVQEIISDNEIDS
                     LVSDPDKVVDIIMYVKNEAAKQDISDDQIRSLIQTAESTAGVSLSEEEENRIIKIVKQ
                     IKDSDIDEEQLRSAVTKAYNKLEEMGIGKEEVKGILHKLADFAKSLFE"
     gene            complement(2030..2902)
                     /gene="rsmA"
                     /locus_tag="ACZPD3_03755"
     CDS             complement(2030..2902)
                     /gene="rsmA"
                     /locus_tag="ACZPD3_03755"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR1415152.1"
                     /translation="MPRPYLGDPKYTIEVLQKYNFAFQKRFGQNFLIDTHVLDKIIDS
                     AQITKDDFVLEIGPGIGTMTQYLAEAAREVAAVEIDKTLLPILDDTLKDWDNVTVINN
                     DILKVDIRQLALEKNQGRPIKVVANLPYYITTPIIMGLFENQVPVDSITIMVQKEVAD
                     RMQVGPGTKDYGALSLAVQYYAKPKIVANVPPNCFMPRPKVGSAVIRLERYEKPPVEV
                     KNEKLMFRIIRASFNQRRKTLVNGLKNSQEIPFSKEQIEQALGMCGLSLSVRGEALTL
                     AQFAQLANAFTEIS"
     gene            complement(2950..5304)
                     /locus_tag="ACZPD3_03760"
     CDS             complement(2950..5304)
                     /locus_tag="ACZPD3_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1415153.1"
                     /translation="MDKEEFRIKLEEINKLVQDKDYKGAMNIVDSIDWRRVKNVRTLC
                     VVGEIYAANGRYEDSKEIFLLAYHKASIGKNILYRLIEISLRMDDINEAEEFFEEYKQ
                     VASNDSTQYILQYKIAKAKNSSLNEQIRILEEYKEQEFTEKWSYELAALYYKAGEKQK
                     CLDLCNEIILWFSEGKYVMKAYDLKMRMGELTGAEKAKFEKQFVPKLLTPEQAKELEK
                     KKTETEVKAQEEPEAEEVEETTENNEPEVQVSMEGIQEKISKGIRDVFGGKTQEEKEE
                     FPEESMDMVNEAGITREDEIPEEIIKSDASQKEPENVPELEAEPEKPGAEPMAAIKMP
                     ELNIPASMKNMELSKPPKVEEVAETKLNMDSFDLEGKELNLEDTILAAASAQGIEIPK
                     EEESKTEEVQEESSETDVDDKEIKAHDISEELEEPDFLSGDIQDIKVADPKPDEEPKK
                     EIPVQPEIEEAEEEDEVTEEDLRRAEEEFLHGPMGNTDLNPEDDTEGTEEAQPLTEEE
                     ELERFIESIQPENGADPRDIVPREKELTDDEKQLFTYFVKVPGMKEQLVDTLYDVQMA
                     AADKTSKTGNIIVMGGKECGKTRLISGLIPAICKELNLEASKVAYVFADQINGKNIYK
                     IFSKLAGGFLVIENANQLTPETVEMLDKAMEVNTDGLTVIVEDEKIGMRKLIARYPKF
                     AKKFTSMINIPVFTNDELVNFARVYTKENGYAIDQMGMLALYNLIGINQKEDSPMNVG
                     AVKELIDAAIAKSQGGIRKFKRNVSKKRIDRDGYIVLYEKDFTK"
     gene            complement(5378..>5972)
                     /gene="recF"
                     /locus_tag="ACZPD3_03765"
     CDS             complement(5378..>5972)
                     /gene="recF"
                     /locus_tag="ACZPD3_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708000.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1415154.1"
                     /translation="LKDLSVQPSLKDTLDIWDIQMAEYGRKIIDKRSEFIKELNETVR
                     KIHGNLTGGLEELNVIYEPDCTAEKLESTICANRERDMRMRLTSAGPHRDDLCVMANG
                     IDIRKYGSQGQQRTAALSLKLSEIYIVKRKIKDTPVLLLDDVLSELDSSRQNYLLDSI
                     SDIQTLITCTGLDDFISHQFQINKVFQVVQGTVSQPV"
BASE COUNT         1588 a         1393 c          928 g         2063 t
ORIGIN      
        1 ctattttcaa acattataac acaatcactt taataatgga aatctgaatt ttcatacaga
       61 tagttcacac aaacggatat ttatatgtta caataatatc atcaaccaac tatagcaaat
      121 ctataaacaa gtcccctgtg agattacaaa aatcaacggg gaactgcctg tatcaggaaa
      181 ggatattatt atacatgaat tatgattctg tccccttgag ggattttaaa gaactgaatg
      241 actgggagac agttattttt gtctatcagt ctgcccttaa acaggtagaa acaaagattg
      301 atattctaaa cggtgaattt cagcacagac ataaatacaa tccaatcgaa catgtaaaat
      361 ccagaatcaa aactccggaa agcattgtaa aaaaactgaa acgacatgga tatgaatcca
      421 gtatcgagaa catggtacgc tatgtaaatg acatcgcagg aatccgtatc agctgttcct
      481 ttacatctga tatctatctg atcgcagata tgatttccaa acaaaatgac ctgacgatcc
      541 ttgcaagacg tgattatatg aaaaatccta aaaagagcgg ttaccgcagt tatcatatgc
      601 tggttaccac accggtcttt ctctccgaca gtatcattga taccaaagta gagattcaga
      661 tccgcacagt tgcacaggat ttctgggcga ctctggagca taagatgcat tacaaatttg
      721 agggagacgg acctgactat atcacaaaag aattaagaga atgtgcgcga tatgtagcag
      781 aactggatac ccgcatggaa gaactcaata atgaaatcca gaaatatgga aaattatcag
      841 ataaaaaata aaaaatcccc gtaaaacgag gagtgccccg gcagtggttt accggggcta
      901 ttattatgaa aaaaatttat gtgtaatgat aacacattac aacgtctttc gtaggttctt
      961 tcgaactatc tatatagtag caatcattta tgaacaaatt atgaactcgc agttaacttt
     1021 tcgtttattt gcacaattat tttatatttt ttaatatttt tttatagaat taccacatat
     1081 ttatagttct tgttttattt tatgcttgtc tatcttgtat atctcccata atcttccata
     1141 actatttcgt cttttcacag ttacgatttg agaatgagca gattttactc aaaaagactc
     1201 tttgcaaaat cagccagttt atgcaggatt ccttttacct cttctttacc aatccccatt
     1261 tcttccagtt tattatatgc cttcgtcaca gcactcctga gctgttcctc atcaatatca
     1321 gaatccttaa tctgtttaac gatctttatg attctgttct cttcttcctc cgaaaggcta
     1381 actcctgcag tactctccgc agtctggatc agcgatctga tctgatcatc tgaaatatcc
     1441 tgttttgctg cctcattttt cacatacata atgatatcaa ctactttgtc cggatctgat
     1501 acaagagaat caatctcatt atcagaaata atctcctgta cagattccga agtatctgct
     1561 gccatcaccg gtactgccgg tgtcgtacat gtaattgctg ccgcaataat aagtattcct
     1621 gcttttttta ttctcatgat ttaattacct ccatactttt gccagtcaat aataagtctc
     1681 tgtcttttca ctgtcagcag attacagaca atgaacaggc attttcattt accggctcat
     1741 tatatcagaa atcttatgcc ttctgtaaaa taaatatccc aatttctgtc ttcataattc
     1801 cttcgacatt tgttcaaatt tttttcacat cttaaacacg attttctcat aattgcagga
     1861 tatactaaag ctataaaaat aataaagcca acgttatttt tataaaattt ctttttcata
     1921 tgttgattgc cggaagaggc aattaactct ccttcctttt aaaaaataaa tttgaacgta
     1981 atataaaaag catccgctta gccaccggat gctttttata tttatctttt tatgaaattt
     2041 ctgtaaatgc atttgccagc tgtgcaaact gtgccagtgt cagtgcttct cctctgacac
     2101 tcagggaaag tccacacatc cccaacgcct gctcaatctg ttctttagaa aatggtatct
     2161 cctgtgaatt cttcagacca ttcaccagtg tcttccttct ctggttaaaa gatgctctga
     2221 taatacgaaa catcagcttc tcattcttta cctcaaccgg cggtttttca tatcgttcca
     2281 gtctgatcac cgcacttccc actttaggtc ttggcataaa acagttgggc ggtacatttg
     2341 ccacgatctt tggtttcgca taatattgca cagccagtga aagcgccccg taatcttttg
     2401 ttccgggtcc tacctgcatt ctgtccgcaa cttctttctg taccataata gtaatagaat
     2461 caacaggaac ctgattctca aacaatccca tgatgatcgg ggtagtaatg taatatggca
     2521 gattggcaac aaccttgatc ggacgcccct gatttttttc cagtgcaagc tggcggatgt
     2581 ctactttgag gatgtcgttg ttgatgactg tcacattatc ccaatctttc agagtgtcgt
     2641 ccagaatagg aagcagtgtt ttatcaatct caactgctgc cacttccctt gctgcttcag
     2701 ccagatactg tgtcatggtt ccgattcccg gtccaatctc caatacaaaa tcatcctttg
     2761 tgatctgtgc agaatctata atcttatcca gaacatgagt atcgatcaga aagttctgac
     2821 caaacctttt ctggaatgca aaattatact tctgaagcac ttctatggta tactttggat
     2881 ctcctagata cggtctgggc ataatcttta ctcctttttc tttattacct gtaactgtac
     2941 aaatacgcat tatttagtaa aatctttctc gtacagaaca atatatccat ctctgtctat
     3001 acgtttctta gatacatttc gtttaaattt acggattcct ccctgtgatt ttgcaatcgc
     3061 agcatctatc agttctttta ctgcgccaac attcatagga gaatcctctt tctggttaat
     3121 tccaatcaga ttatacagcg caagcattcc catctgatca atagcataac cattctcttt
     3181 tgtataaacc cttgcaaaat tgacaagttc atcattggta aacacaggaa tattgatcat
     3241 tgaagtaaac ttctttgcga atttcggata tctggcaatc agttttcgca taccaatctt
     3301 ctcatcttcc acgattactg taagtccgtc tgtattaact tccatggctt tgtcaagcat
     3361 ctctactgtt tccggtgtca gctgatttgc attttcgatc acaagaaagc cacctgccag
     3421 ctttgaaaaa atcttataaa tattttttcc gttgatctga tctgcaaaaa catatgctac
     3481 ttttgacgct tccagattca gttctttaca gattgccgga atcaggcctg agatcagtct
     3541 ggtctttccg cattccttac cgcccatcac aataatattt ccagttttag aagtcttgtc
     3601 tgctgcagcc atctgtacat catataaagt atccacaagc tgttctttca tacccggaac
     3661 ctttacaaaa tatgtaaata actgcttctc atcatctgta agttcttttt ctcttggtac
     3721 gatatccctt ggatctgctc cattttccgg ctggatagat tctataaaac gttccagctc
     3781 ctcttcttct gtcaggggtt gggcttcttc cgttccctct gtatcatctt ccggattcag
     3841 atctgtattc cccatcggtc catggagaaa ttcctcttca gctcttctga gatcttcttc
     3901 tgtaacctca tcttcttctt cagcttcttc tatttccggc tgcaccggta tttccttttt
     3961 cggttcttca tccggctttg gatcagcaac ctttatatct tgtatatctc cgctgaggaa
     4021 atccggttct tccagttcct ctgaaatatc atgggctttt atttctttat catcaacatc
     4081 agtttctgaa ctttcttcct gtacttcttc tgtttttgac tcttcttcct tcggaatctc
     4141 aattccctgc gcactggcag cagcaaggat cgtatcctcc agattcaact ctttcccttc
     4201 cagatcaaaa gaatccatat tcagtttcgt ctcagcaact tcctcaactt tcggcggttt
     4261 gctcagttcc atatttttca tggatgccgg aatatttaat tcaggcatct taattgcagc
     4321 catgggttct gcccccggtt tttcaggttc tgcctccaat tccggaacat tttcaggttc
     4381 tttctgcgac gcatcactct ttatgatttc ttccggaatc tcatcttccc tggtgatacc
     4441 ggcctcgtta accatatcca tggattcttc cggaaactct tctttctctt cctgagtctt
     4501 acctccaaag acatccctga tcccctttga aatcttttcc tgaatgcctt ccatgctgac
     4561 ctgaacttcc ggctcattgt tttctgtcgt ttcttccact tcttcagctt caggctcttc
     4621 ctgcgctttt acttctgttt ccgttttttt cttctccagt tctttcgcct gctctggtgt
     4681 aagaagtttc ggaacaaact gtttctcaaa ttttgctttc tcggcacctg tcagctcacc
     4741 cattctcatt ttaagatcat aggctttcat aacatatttg ccttcactga accagagaat
     4801 gatttcattg cataaatcca gacatttctg tttttctcca gctttgtaat acagggctgc
     4861 cagttcatat gaccatttct ctgtaaattc ctgttcttta tactcttcca ggattctgat
     4921 ctgctcattt aatgaagaat tttttgcctt tgcaatcttg tactgcagaa tatattgtgt
     4981 actgtcattc gaagccacct gcttatactc ttcaaaaaat tcttctgctt cattgatatc
     5041 atccattcgc agagagatct caatcagacg gtaaagaata ttttttccaa tagatgcctt
     5101 atgatatgca agaaggaaaa tctccttact gtcctcatat cttccgtttg ctgcatatat
     5161 ttcaccgaca acacataagg tgcggacatt tttgacgcgt ctccagtcta tgctgtctac
     5221 aatgttcatg gcacctttat aatctttgtc ctgaaccagc ttattaatct cttcaagttt
     5281 tatccgaaat tcttctttat ccaatgcatt caccaacttt ccataattac gctcagtgta
     5341 tcataagccc gtacccttgt caaaagattg tttcttctta cacaggctgt gatactgttc
     5401 cctgaactac ctgaaatacc ttatttatct gaaactgatg actgataaaa tcatccagcc
     5461 ctgtacatgt tatcaatgtc tggatatcac tgatactgtc cagaagataa ttctgcctgc
     5521 tgctgtccag ctcggacaga acatcatcca aaagcagaac cggtgtgtct tttatttttc
     5581 tctttactat atatatttct gataatttaa gtgaaagtgc cgctgtcctc tgctgtccct
     5641 gagaaccgta tttcctgata tcaatcccat ttgccattac acacaggtca tctctgtgtg
     5701 ggcctgcgga tgtcagtctc atcctcatat ctctttctct gttggcacaa atggtacttt
     5761 ccagtttctc tgctgtacag tccggttcat aaataacgtt cagttcttcc aatccccctg
     5821 taagattacc atgtattttt cgaaccgttt catttagttc tttaataaat tcactgcgct
     5881 tgtcaataat cttccgtcca tattcagcca tctgtatatc ccagatatcc agtgtatctt
     5941 taagtgaagg ctgaacactg agatccttca gc
//
LOCUS       DBMDXG010000085         5965 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3367_length_5965_cov_1.471351, whole genome shotgun sequence.
ACCESSION   DBMDXG010000085 DBMDXG010000000
VERSION     DBMDXG010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3367_length_5965_cov_1.471351"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="ACZPD3_03770"
     CDS             complement(<1..302)
                     /locus_tag="ACZPD3_03770"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR1415155.1"
                     /translation="MAKKRNIIVGQSGGPTAVINSSLAGVYKNAIERGFDKVYGMLHG
                     IQGLLDEQYIDLSTQIHSELDIELLKRTPSAFLGSCRYKLPEIHEDKTIYEKIFEIL"
     gene            788..1663
                     /locus_tag="ACZPD3_03775"
     CDS             788..1663
                     /locus_tag="ACZPD3_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR1415156.1"
                     /translation="MIHTIENDYLRVSVDDHGAELCSIFDKVHNREVIWQADPAYWKR
                     HAPVLFPNVGRHFEDHYRINGVEYPSSQHGFARDSEFTCVDMTADSITHRLKSSDATR
                     ENYPYDFELKIKHVLEKNQVSVCWEVISLNDETMYFTIGGHPAFNVPAGGIGSQEQYH
                     LTFDGRDSLSYLLIDMSSGTAVADKAYTLELENGSCLIDAHMFDKDALIFDDQIEKAG
                     IAFPDGTPYVELICHGFPSFGIWSVPGSPFVCLEPWMGRCDDFGFKGELPEKKYINTL
                     DKDEIFTASYEIKIY"
     gene            1812..2684
                     /locus_tag="ACZPD3_03780"
     CDS             1812..2684
                     /locus_tag="ACZPD3_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR1415157.1"
                     /translation="MTREEFQKLTQDVVLLDGATGSNLMAAGMPRGICTEAWIMEHKE
                     VLQNLQKAYVEAGSQIVYAPTFGGNRYSLGLHGLQDKLAEMNHALVNISREAVGHKVY
                     VAGDITTTGKMMEPAGDLTYEMAYETYCEQIKVLEDAGVDLIAAETMINIEETLAALD
                     AAASVSSLPVMCTMTVEADGSIFSGGNAVEAAIALEGAGAVAVGINCSVGPDQLVSVV
                     RNIKENVSIPVIAKPNAGMPTIDDQGNAIYSMDAKSFAEHMKVLIENGASVVGGCCGT
                     TPEFIREISRSLGR"
     gene            complement(2941..4074)
                     /locus_tag="ACZPD3_03785"
     CDS             complement(2941..4074)
                     /locus_tag="ACZPD3_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1415158.1"
                     /translation="MKSKVVLLPCREYDEEKIYMLLKQGLDFLGGVETLIPKDAKILL
                     KPNLLKKAEVEKAVITHPVVVGVFAGILRESGYENIVLADSCGHGTTQVVIRGTGMDT
                     YLEKYHIPAVDYSEGVKTAYPQGVQAKEFILPKELLEQDCVISLSKMKTHALERITGA
                     VKNSYGFVYGFHKAKGHTQYPSADSFARMLIDLNKCVAPKLYVMDGIVAMEGNGPGSG
                     DPVPMNVLLMSTDPVALDSVFSRLVYLKPEMVPTNYHGEKMGLGTWKEEEITLLTPDG
                     EISMAEAVKKYGNPAFNVDRTEVRKNIWTRMAGALNIFQKKPYIEADKCVRCGICVQS
                     CPVPGKAVDFRKGKGKPPVYDYRKCIRCFCCQEMCPKKAIKVK"
     gene            complement(4080..5714)
                     /gene="ptsP"
                     /locus_tag="ACZPD3_03790"
     CDS             complement(4080..5714)
                     /gene="ptsP"
                     /locus_tag="ACZPD3_03790"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR1415159.1"
                     /translation="MDVYKGTGAFSGIAIGKILYYHKSEYQMRQYEITDVKTELNIFR
                     QARTRVMEQLEDLYEKNCIIQGTQAEIFLRQKNLLEGKSFQRAIESVIQSEKVNSAYA
                     VMTTRDEMLKTFRTLEEPAIKERLDNMREISDRLIGELGGISPRIDLGDEPVIVVTES
                     ITPTELMEMDKDKLMAIVTHHGSDMSHAAIMAKTMNIPALLEIDTDSEWDGRQAIVDG
                     YTGTLYIDPDEEVKKEYEIRRQADKEEREELLKLRREPDITKDGREIEIYANIGNMDD
                     LNSVLYYGAAGIGLLRSEFQYLGRENYPRENELFLAYKKIAETMGEKLTVIRTADLGA
                     DKQAEYLNIPDETNPIMGNRGIRLCLDRKRMFKAQLRAIFRASAYGNLALMYPMISSE
                     EEMDEIEEIIREVKIGLDEKGIPYKHIKTGIMIETPAAVMISRELARRVDFLSLGTND
                     LSQYTLAMDRQNPLLRKKYNDHHPAVLRMIQMVIEAGHAENRRVCICGELAADTALTE
                     EFLRMGVDCLSVVPACILPVRKALRQVDLSGDDKGA"
     gene            complement(5740..>5965)
                     /locus_tag="ACZPD3_03795"
     CDS             complement(5740..>5965)
                     /locus_tag="ACZPD3_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1415160.1"
                     /translation="AAYSFMDYIMQPEVAAKCTDYIGYYSTNKAADELVNPDLVVPDD
                     VTKGEIVQNVSQDADAQYQKNWTEFKAACD"
BASE COUNT         1506 a         1464 c         1204 g         1791 t
ORIGIN      
        1 aggatttcaa aaatcttctc ataaatagtc ttgtcttcat ggatctccgg aagcttatag
       61 cggcaggaac ccaaaaatgc ggacggtgtc cttttcagaa gttcaatatc aagctcagaa
      121 tgaatctgtg tggaaaggtc aatatactgc tcatcaagaa gtccctggat tccgtgaagc
      181 attccgtata ctttgtcaaa tcctcgttca atggcattct tataaactcc ggcaagactg
      241 gaattgatta ctgcagtagg tcctcctgat tgtccaacaa tgatatttct ttttttcgcc
      301 atagcaatga cctccttgtt ttcacgtttt gttcattatg tcttagagtg tgtctgaaaa
      361 atgctttacg caagatgtgt gtcacaattt ttcagacacg ctctggtccc gcaggactcg
      421 ttgaggtaaa ttgtaacaaa tattttttta ttcgtcaatc atttattgtg gttcttacac
      481 atatgatttc tgtatttcgt cactatatca gtgtattttt caatttacca cgacatttca
      541 gatctatttt tgtatattta taactataat taatccggcc catgtgttat acttatagac
      601 agattgattc cggcatcaac ttatagcaat caaaaccttt ttaaaaattg ccgggcatca
      661 atgtccgtga ctattcggta ccttcgcagt tacgaaccaa aatgcatttc aaatcacctt
      721 cggtgatttg cagaatactg ccagctgaac agttacacaa tatttatcga tcaaggagga
      781 ttcatttatg atccatacaa ttgaaaacga ctatcttaga gtatctgtag acgatcatgg
      841 tgctgagctt tgcagcattt ttgataaagt acataacaga gaagtgatct ggcaggcaga
      901 tccggcttac tggaaacgcc acgcaccggt tcttttccct aatgtgggac gtcattttga
      961 ggatcactat cgtattaatg gagtcgaata tccctccagt cagcatgggt ttgccaggga
     1021 cagcgaattt acctgcgtgg atatgactgc tgattccatc acccataggc tgaaatcttc
     1081 tgatgccaca agagaaaatt atccatatga ttttgagctg aagatcaagc atgttctgga
     1141 gaagaaccag gtcagtgtat gctgggaagt gatcagcctt aatgatgaaa ccatgtattt
     1201 tacaattggc ggccatcctg cattcaatgt acctgccgga ggaattggct cccaggagca
     1261 atatcatctt actttcgacg gccgggattc cctctcctat ctgctgatcg acatgtcatc
     1321 aggcaccgcg gtcgcagata aagcttatac actggagctt gagaatggtt cctgccttat
     1381 tgatgctcac atgtttgaca aagatgccct tatttttgat gaccagattg aaaaggccgg
     1441 catcgctttc cctgacggaa ctccttatgt ggaactgatc tgtcatggat ttccaagctt
     1501 tggcatctgg tccgtcccgg gttctccttt tgtatgtctg gaaccatgga tgggacgctg
     1561 tgatgatttt ggatttaagg gcgagcttcc ggaaaagaaa tatatcaaca ctctggacaa
     1621 ggatgaaatt tttactgcat catatgaaat aaaaatttat taattttctc catttatcct
     1681 gaaagctgtg ctataatgtc acagtatatg aaagtcatcc tgtccggtat ccacgaattt
     1741 acccatgcag acatgactga ttcatggcag ccactcagga ctttcataga aaaacacagg
     1801 aggaaatttc catgactaga gaagaatttc agaagctgac acaggatgtt gtactgctcg
     1861 acggtgctac cggttccaat cttatggccg ccggaatgcc aagaggaatc tgtacagaag
     1921 catggatcat ggagcacaaa gaagttctgc agaatctgca gaaggcttat gttgaagcag
     1981 gaagccagat cgtatacgca cctactttcg gcggaaaccg ttacagtctc ggacttcacg
     2041 gactgcagga taagcttgcg gaaatgaacc atgcacttgt aaatatttcc agagaggcag
     2101 taggacataa ggtctatgtt gccggtgata ttaccacaac aggcaagatg atggagcctg
     2161 ccggtgatct cacctatgag atggcatatg aaacatactg tgaacagatt aaggttcttg
     2221 aggatgcagg tgttgatctg atcgctgcgg aaaccatgat caatatcgag gaaacacttg
     2281 ctgcactgga tgctgctgca tccgtaagtt ctcttcctgt aatgtgtaca atgaccgtag
     2341 aagctgacgg aagcattttc agcggaggaa atgctgtgga ggctgcgatt gcccttgagg
     2401 gtgcaggtgc tgtggcagtt gggatcaact gttctgtagg acctgaccag ctggtatccg
     2461 ttgtccgcaa tatcaaagag aacgtatcca ttcctgtcat tgccaaacct aatgccggta
     2521 tgccgacgat tgatgatcag ggaaatgcca tttacagtat ggacgccaaa agctttgctg
     2581 aacatatgaa ggttctgatc gaaaatggtg cgtctgttgt tggaggatgc tgtggtacta
     2641 ctccggaatt catccgcgag ataagcagaa gcctcggaag atagattttg tatcttcgac
     2701 agccatagtc agacgaataa cgaatattcg cagccaaagc agggaacttt cctgattcgg
     2761 ttaaagaggg atatgactca aaaaagcaca taacttagga tagaatctgt ctcaatccga
     2821 gatattctca tcctctgtta tgtgcttttt ggtaattgtc cggctttcct ctgtcctgat
     2881 tctgcgaaaa taccatggca attctataaa caattgcgct ttttattatc tgcttttcat
     2941 ctacttcact ttgattgcct tcttcggaca catctcctgg cagcagaaac agcggataca
     3001 ctttctgtaa tcatataccg gcggctttcc tttgcctttt ctgaaatcca cagctttccc
     3061 cggaaccgga cagctctgca cacagatccc gcagcggaca catttatcag cttctatata
     3121 tggttttttc tgaaaaatgt ttaacgctcc cgccattctt gtccagatat tctttcgcac
     3181 ttctgtgcgg tctacattaa aagccggatt tccgtatttt ttcactgcct ctgccatgga
     3241 aatctccccg tctggtgtca gaagggtaat ctcctcttct ttccatgtac caagtcccat
     3301 tttctcgcca tgatagttcg tcggaaccat ctccggtttc agataaacca gacggctgaa
     3361 tacactgtcc agtgcaaccg gatctgtgga catcagcagt acattcatag gaaccggatc
     3421 tcccgaaccc ggtccgttgc cctccatggc cacgatccca tccatcacat acagcttcgg
     3481 tgccacacat ttattcagat caataagcat tctggcaaag ctgtctgcac tcgggtactg
     3541 cgtgtgcccc ttcgctttat ggaatccata tacaaaccca tagctgttct taaccgcacc
     3601 ggtgatacgt tctaatgcat gtgttttcat cttgctcagg gaaatcacac agtcctgctc
     3661 caacagttct tttggcagga taaattcctt tgcctggact ccctgcggat aagcagtttt
     3721 cacgccctca gaataatcaa cagccggaat atgatatttc tccagataag tatccattcc
     3781 tgttccgcgg atcactacct gagtggttcc atgaccgcag gaatctgcaa gcacaatatt
     3841 ctcataccca ctttcacgca ggattcccgc aaaaactcct actacaaccg gatgtgtgat
     3901 cactgctttc tctacctctg cttttttcag cagattcggc ttcagcagga tcttcgcatc
     3961 ctttggaatc agagtctcca ctccacccag aaaatccagc ccctgtttca gcagcatata
     4021 gattttttct tcatcatatt cccggcaggg aagtaatacc actttacttt tcaccaaatt
     4081 cacgcacctt tatcatctcc ggacagatcc acctgtctca gtgcttttct taccggcaaa
     4141 atacatgccg gcactacaga gagacagtcc acacccatcc tcagaaattc ttctgtcagg
     4201 gcagtgtctg ctgcaagttc cccgcagata cagacacgtc tattttctgc atgtcccgct
     4261 tcgatcacca tttgaatcat acgcagcact gccgggtgat ggtcattata tttcttgcgc
     4321 agaagcggat tctgtctgtc catcgccagt gtatactgac tcaggtcatt ggttccaaga
     4381 ctcaggaaat ccactcttct ggcaagctcc cggctgatca tgactgctgc cggagtctcg
     4441 atcatgattc ctgtcttaat atgtttatac ggaattcctt tttcatccag cccgatcttt
     4501 acttcccgga taatttcttc aatctcatcc atttcctctt ctgagctgat catcggatac
     4561 atcagcgcca ggtttccata tgcactcgcc ctgaaaattg cccgaagctg tgccttgaac
     4621 attctttttc tgtcaaggca gagacggatt cctctgttcc ccatgatcgg atttgtctcg
     4681 tccgggatat tcagatattc tgcctgcttg tctgctccga gatctgccgt acggatcaca
     4741 gtgagctttt ctcccatggt ctccgcaatc tttttgtatg caagaaacag ctcattttca
     4801 cgaggataat tttctcttcc aagatactgg aattcactgc gcagaagacc aatcccggca
     4861 gctccgtaat acagcacact gttcaggtcg tccatatttc cgatattggc atagatttca
     4921 atttctctgc catcttttgt gatatccggt tctctcctta atttcagaag ttcttctctt
     4981 tcttctttat cagcctgcct gcggatctca tattcttttt ttacctcttc atccggatca
     5041 atatacaaag ttccggtata tccgtccaca atagcctgcc gtccgtccca ctccgaatct
     5101 gtgtcaattt ccagcagagc cggtatattc attgtttttg ccataatggc agcatgagac
     5161 atgtcggaac catgatgggt aacaatagcc atcagtttat ccttatccat ctccatcagt
     5221 tctgttggcg tgatactctc cgtcacaacg atcacaggct catccccaag atctatcctg
     5281 ggagagatac ctcccagttc accgatcagg cggtcggaaa tctcccgcat attatccagc
     5341 cgttctttaa tcgcaggctc ttccagagtt ctgaaagtct taagcatctc atcccttgta
     5401 gtcattaccg cataggcaga atttactttt tcactctgta tgacactctc aatggcacgt
     5461 tgaaagcttt ttccctccag cagattcttc tgtctgagaa aaatctctgc ctgggttccc
     5521 tgaattatgc agtttttttc atataaatcc tccagctgtt ccatgactct ggtgcgcgcc
     5581 tgccggaaaa tattcagctc tgttttcaca tctgtgatct cgtactggcg catctgatat
     5641 tcgcttttat gataatacag tatcttaccg atggcaattc cggaaaaggc accggttccc
     5701 ttgtaaacat ccatattaac tccttagttg attaacccct tagtcgcagg cagccttaaa
     5761 ttcagtccag ttcttctgat actgtgcgtc tgcgtcctgg cttacattct ggacgatctc
     5821 gccctttgta acatcatccg gaactaccag atccggattt accagctcat ctgctgcttt
     5881 atttgtgctg taatagccga tatagtctgt gcatttcgct gcaacctccg gctgcatgat
     5941 gtaatccatg aaagaatatg ccgca
//
LOCUS       DBMDXG010000086         5791 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3477_length_5791_cov_2.179613, whole genome shotgun sequence.
ACCESSION   DBMDXG010000086 DBMDXG010000000
VERSION     DBMDXG010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3477_length_5791_cov_2.179613"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="ACZPD3_03800"
     CDS             complement(<1..606)
                     /locus_tag="ACZPD3_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415161.1"
                     /translation="MTDILCPVLELSVIIPGILLAYLPVKNYLRQTPLKLTAWLLPLL
                     LGICILGGAVCCALQIPTRWFLFPLLPVIMLIYHKTLKISVWKSVSIFLAVFAVFTCV
                     KSLSRAVNALMTADLHITENELWLHTGAGIFYNVICLLFVLAAWYPACHCVQTMVTDE
                     NFAQTWYVFWILPVIFIGVNLFMIPKCRGTLYTGRVLQCYIV"
     gene            complement(608..1324)
                     /locus_tag="ACZPD3_03805"
     CDS             complement(608..1324)
                     /locus_tag="ACZPD3_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1415162.1"
                     /translation="MRIAIVDDISEERTLLHNRLESQFSRRNVHTDIFEYENGETFLT
                     SAKECPFTVAFLDIYMNGSNGIDTAKELRRSDTDCLLIFTTTSTDHALEGFQVRALHY
                     LVKPYSENDISVLADEILSRIPDSGKYIDVKVNGSNIQIPFRKIIYAEHFSHMIHIHT
                     AGERELVTRQSFDSFITSLKMDSRFYRCNRGVVINLEHAVDFDGSGFRLDNGSNVPVS
                     RKLLKNARQTFMEFLFQRRS"
     gene            1710..2084
                     /locus_tag="ACZPD3_03810"
     CDS             1710..2084
                     /locus_tag="ACZPD3_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1415163.1"
                     /translation="MYCIAILTDQEQEGQNCAEYIRNYCTEKKVFPLIEIYQNQEQFF
                     GRIRKTVPAVVFLALPGVSGLNAAEHLRSLYPKCGIIWCSDLDFSLHAFRMRIEYFFM
                     KPVEEQKIKEGLSIWFERRNVI"
     gene            2111..4177
                     /locus_tag="ACZPD3_03815"
     CDS             2111..4177
                     /locus_tag="ACZPD3_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415164.1"
                     /translation="MKKRALILAMIAFIAATTSEPSINMVRAEETQVEETQTEENAGQ
                     TSELPIKTLTPKVMDVNPYMAASDSNIHHDCYNTDSTDEVLPVDIYSEINVSYEKVNP
                     NASPAVFFDSYGHAVVPLLGGLAIRDINAEETQTLGYFSPKQHDNGDYLIQSSYSFVD
                     ESNRLVCPTNNNHVLMLKATDEDGNVLPEFEKVLDIDIKAAAEAALGKTLDQNLLSVV
                     FDYEGNLWFATGGFRIYPDRKQQGTFGYVSRAAIDKILNGEDVDLSDAVFVYELEPGE
                     GAENGIAASKEGAVILTNLKCYLLQADNGVKKVWETSYKSVGAKESKEGDETTGGGLA
                     WGGGCSPSLTNDLVMFTDNQDPVNLIAVDMKTGEQVASMPVIDELPEGTHVSVENSAI
                     VYDDGEGTVSTIVCNWFGAGSAKLGEADNDSSIQSYENIYDVGWLRQGNKMIAPGIER
                     VDTVKTEDGYEMKSIWCRSDLSDTSMMKLSTATGYIYGYVQDMETGMWQYIMLDFETG
                     ETAFTMDISDKPGYNNMAIGMYAGNSGNALYCPTGYLELLRLQDRFVYLPEMPYRKVD
                     LDQAMRNVLSQEKFAADGGQGDVEGWLNTITVENVHPNTTVAIRMKGISGETGSLKLY
                     AYGTDGTLKEVPAEKWHIQTEDGETPDTLSEDVLYEVHMTVEDGGDFDLSETEKEIKI
                     SAVLGI"
     gene            4349..4669
                     /locus_tag="ACZPD3_03820"
     CDS             4349..4669
                     /locus_tag="ACZPD3_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR1415165.1"
                     /translation="MYDTKTRIALVKKRMHEYHRRQERRAVCRLSVLCTLLFLSLVGA
                     MGIMQSQPINVTGMYGTILLHEDAGGYVLVAVISFNVAVVITALCIKFRKRGQKSQDA
                     EDHV"
     gene            4690..>5791
                     /locus_tag="ACZPD3_03825"
     CDS             4690..>5791
                     /locus_tag="ACZPD3_03825"
                     /inference="COORDINATES: protein motif:HMM:NF039179.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415166.1"
                     /translation="MRKKLLAGILALALCSTNMPPQTIFAEEFTSGNQDVVSEEETPE
                     IFTNEELEAAGETDEELSVFSSEEVPEFNDAPDEAMAAAENEQAGEIDLTTSNKVVNG
                     VYTISSAGDHKFICSQETGNRIVVDGTNISEQDNINIYLDNVNINTSVGPALRINLNV
                     EATVTIHLTGTNSLITKDNWYAGLQKDNEARLIIKTNNSDATAGILNARSIDGDGAGI
                     GGGYHGFGSCSNIFIDSCSVIASSKHGAGIGGSKGHAGSDITINNSSVTASSTDGAGI
                     GGGGYGTGSGSNITIKSSSVTASSTDGAGIGGGGYGTGSVSGITIKSSSVTASSTNGA
                     GIGGGGYGAGSVSDITIKSSSVTATSTHGAGIG"
BASE COUNT         1742 a         1039 c         1443 g         1567 t
ORIGIN      
        1 aacaatataa cactgcagga cgcgcccggt atataaagta ccgcggcatt taggaatcat
       61 aaaaagattc actccgatga agattaccgg aagaatccag aatacatacc aggtctgggc
      121 aaagttctcg tctgtgacca ttgtctgtac acagtggcag gcaggatacc aggctgccag
      181 aacaaacagc aggcagatga cattatagaa aattccggca ccagtgtgca accacagttc
      241 gttttctgta atatgcaggt cagcagtcat aagtgcattg actgcccggg acagactttt
      301 gacacatgta aaaactgcga agactgccag aaaaatactg acggatttcc agacagagat
      361 ttttaatgtt ttatggtaaa tgagcataat gacaggcagc agaggaaaca ggaaccatct
      421 ggtcggaatt tgcagggcac agcaaactgc ccctcccaaa atacagattc ccagaagaag
      481 gggaagaagc catgctgtca gcttaagcgg tgtctgcctc agatagttct ttacaggcag
      541 atatgccagc agtataccgg gaatgattac ggaaagttca agtacagggc aaagtatgtc
      601 agtcatttca ggacctcctt tggaacagaa attccataaa tgtctgtctg gcgtttttga
      661 gcagcttccg gctgacaggg acattgcttc cgttatcaag gcgaaagcct gatccgtcaa
      721 aatcaactgc atgctccaga ttgatcacaa cgccccggtt acaccgatag aaacgtgagt
      781 ccattttcag ggaggttatg aaggaatcaa aggactggcg ggtgaccagt tccctttcac
      841 cggcagtgtg gatgtggatc atatgtgaaa aatgctctgc gtagattatt ttccggaagg
      901 gaatctgaat attactgccg tttaccttta catctatgta tttcccggaa tccgggatgc
      961 gggaaaggat ttcatctgcc agtacggaaa tatcattttc actgtagggc tttacaagat
     1021 agtggagagc cctgacctga aatccctcta aagcatggtc ggtagaagtt gttgtaaaga
     1081 tcagcagaca gtcagtatct gatctgcgaa gttcttttgc tgtatcaatt ccgttagaac
     1141 cattcatata aatatccaga aacgctacag tgaaaggaca ttccttcgct gaagtgagaa
     1201 aagtttctcc attttcatat tcaaatatat ctgtatggac attgcgcctg gaaaactgag
     1261 attccagtct gttgtgaagc agtgtccgtt cttctgaaat atcatcaaca attgcaattc
     1321 tcatagtaaa tcttccttta tttataaaat gatgttaggg tcaaaaggca ttttgcccct
     1381 aacttgatat ctacgaattg cttcgctgca aagcagctcc cgcaattctc aaacattcga
     1441 aatccgctga tttactacgt aaatcgctgc tttctcatgt ttggcgggtc ccaagttcaa
     1501 aagtcttatc gtgcaagcac gaacgacttt ttgacctttt gaccctgata ttataaaatt
     1561 ctgctataag tatatcacaa aacaatacca tagatttaca attcgtgtca ttttctgtcg
     1621 tttcgtgaca gaattattga aaaaatttct gactttttta taatgaggat aacaaatggg
     1681 agtacatcaa atggaagagg agggatgtcg tgtattgtat tgccatatta actgatcagg
     1741 aacaggaagg acagaactgt gcggaatata tcaggaatta ctgtacagaa aagaaagttt
     1801 ttccattgat tgaaatctat cagaatcagg aacagttttt tgggcggata cgaaaaacag
     1861 taccggcagt ggtgtttctt gcattgccag gtgtgtctgg gctgaatgcg gcagaacatc
     1921 tccgttccct gtatccgaaa tgtgggatta tctggtgcag tgatcttgat ttttcgcttc
     1981 atgcgttcag gatgcggata gaatattttt ttatgaaacc tgtggaagag cagaaaataa
     2041 aagaaggact ctccatctgg tttgaacgca gaaatgtaat atgacaaaaa cactcaggag
     2101 gaaaacaatt atgaagaaaa gagctttaat tcttgcaatg atcgctttta tagcagctac
     2161 aacatctgaa ccgtcaataa atatggtacg tgcagaggaa acacaggtag aagaaacaca
     2221 gacagaggaa aatgcaggtc agacatcaga actgcctata aagacactta caccgaaggt
     2281 tatggatgtg aatccttata tggcagcaag tgattccaat attcatcatg actgctataa
     2341 tacagattca acagatgagg tattgcctgt tgatatttat tctgagatca atgtttctta
     2401 tgaaaaagtt aatcctaatg cttccccggc agtctttttt gattcttacg gacatgcagt
     2461 agttccgttg cttggtggac tggcaatccg tgatattaat gcagaggaaa cacagacact
     2521 gggatatttt tctccaaaac agcatgataa tggagattat ctgatccaga gttcctattc
     2581 ctttgtagat gagagtaacc gtctggtgtg tccgaccaat aataaccatg tactgatgct
     2641 gaaggcaaca gatgaagacg gaaatgtact tccggaattt gagaaagtgc tggatattga
     2701 tattaaagca gcagcagagg ctgcacttgg aaaaacactg gatcagaatc tgttatctgt
     2761 agtttttgac tatgagggaa atctctggtt tgcaacagga ggcttccgca tttatcctga
     2821 cagaaaacag cagggaacct ttggctatgt ttcacgtgca gctatcgata agatcttgaa
     2881 tggagaagat gtggatctgt cagatgcagt gtttgtatat gaactggaac cgggagaggg
     2941 tgcagagaat ggtattgcgg catctaagga gggtgcggtc attctgacaa atctgaagtg
     3001 ttatctgctc caggcggata atggtgtgaa aaaagtctgg gaaacttcct ataaaagtgt
     3061 gggtgccaag gaaagtaaag aaggcgatga gacgacaggg ggcggtcttg catggggagg
     3121 tggatgttcc ccgtctctga ccaacgatct ggttatgttt acagataatc aggatccggt
     3181 aaatcttatt gcggtagata tgaaaacagg agaacaggtt gcgagtatgc ctgttataga
     3241 tgagcttccg gaaggtacac atgtgtctgt ggaaaattct gcaattgtat atgatgatgg
     3301 agagggaaca gtaagcacaa ttgtatgtaa ctggtttggt gcaggaagtg cgaagcttgg
     3361 agaagcagat aatgattcct caatacaaag ctatgagaat atttatgatg tgggttggct
     3421 gagacagggc aataaaatga ttgcgccggg tatagaacgt gtggatacag ttaagacgga
     3481 ggacggatat gagatgaaga gtatctggtg cagaagtgat ctgtctgaca cttctatgat
     3541 gaagctgtcc actgcaacag gatatattta cggatatgtt caggatatgg aaactggtat
     3601 gtggcagtat atcatgcttg attttgagac aggcgagact gcatttacca tggatatttc
     3661 tgacaaaccg ggatacaaca acatggctat cggtatgtat gcgggaaaca gcggaaatgc
     3721 gctctattgt ccgaccggct atctggaact tctgagactg caggacaggt ttgtatatct
     3781 gccggaaatg ccttaccgca aggtggatct ggatcaggca atgcgcaatg tgttaagtca
     3841 ggagaaattt gcggcagacg gcggccaggg agatgtggaa ggatggctga atacgatcac
     3901 agttgagaat gtacatccga atacgaccgt tgcaatacgt atgaaaggaa tctcagggga
     3961 aacaggttct ttgaagctgt atgcatatgg tacagatggt accttgaaag aagttccggc
     4021 agagaaatgg catatccaga cagaagatgg tgaaacaccg gatacattat ccgaagacgt
     4081 actgtatgag gtacacatga ctgtggaaga tggtggtgac tttgatctca gcgaaacgga
     4141 aaaagagatt aagatatccg ctgttctggg aatctgaagg agattcgata caataatata
     4201 acctgatttt ccgtttttgg gagctgtttc ggcagctctc aaatttttta aaaaaatttg
     4261 attttcaggt gagagttttt cggttttttt gtacctatat aagtgaaaga ctttttttga
     4321 ctgttttttg ggaaggaggt tggaaggtat gtatgatacc aaaacccgta tcgcactggt
     4381 aaaaaaacga atgcatgaat atcaccgccg ccaggagcgg cgcgcagtct gccggctgtc
     4441 tgttctgtgt accctgttat tcctgtctct tgtgggagca atggggataa tgcaaagcca
     4501 gccaataaac gttacgggaa tgtacggcac aattctgctg catgaggacg caggcggcta
     4561 tgtgttggtg gctgtcattt cctttaatgt ggcagtcgtg attaccgcat tatgtatcaa
     4621 attcagaaaa agaggacaaa agagtcaaga cgcagaagat catgtataaa gaaaggaaag
     4681 gtggaaagca tgaggaaaaa gttattagcc gggattctgg cacttgcatt gtgttctaca
     4741 aatatgcccc cccagaccat atttgcggaa gaatttactt ccggaaatca ggatgtagtg
     4801 tctgaggaag aaactccgga gatttttacc aatgaggagc tggaagcggc aggagagaca
     4861 gatgaggaac tgtctgtatt cagttcagaa gaggttccag aatttaacga tgcccccgat
     4921 gaggcaatgg ctgctgcgga aaatgaacaa gctggagaaa ttgatttaac tacgagcaat
     4981 aaagttgtga atggagttta tactataagt tcggcaggag atcacaaatt catatgtagc
     5041 caggaaacag gaaatagaat tgtggttgat ggaacaaata tttcagagca agacaacatc
     5101 aacatttatc ttgataatgt aaatatcaat acatctgttg gtccggcgct gcgaataaat
     5161 cttaatgttg aagctacagt cactatccat ttaacaggca caaatagtct tataacaaag
     5221 gataactggt atgccggttt acagaaagat aacgaagctc ggcttataat aaaaaccaat
     5281 aattcagacg ctactgcagg aatcttaaat gctcgtagta tcgatgggga tggcgccgga
     5341 attggtggcg gctatcacgg cttcggctct tgcagtaata tttttattga tagttgttct
     5401 gtaattgcta gttctaagca tggtgctgga attggtggca gcaagggaca cgctggcagt
     5461 gatattacca ttaataatag ttctgtaact gctagttcta cggatggtgc cggaattggt
     5521 ggcggcggtt acggcactgg cagtggcagt aatattacca ttaaaagtag ttctgtaact
     5581 gctagttcta cggatggtgc cggaattggt ggcggcggtt acggcactgg cagtgtcagt
     5641 ggtattacca ttaaaagtag ttctgtaact gctagttcta cgaatggtgc cggaattggt
     5701 ggcggcggtt acggcgctgg cagtgtcagt gatattacca ttaaaagtag ttctgtaact
     5761 gctacttcta ctcatggtgc cggaattggt t
//
LOCUS       DBMDXG010000087         5777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3491_length_5777_cov_1.897463, whole genome shotgun sequence.
ACCESSION   DBMDXG010000087 DBMDXG010000000
VERSION     DBMDXG010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3491_length_5777_cov_1.897463"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            154..4377
                     /locus_tag="ACZPD3_03830"
     CDS             154..4377
                     /locus_tag="ACZPD3_03830"
                     /inference="COORDINATES: protein motif:HMM:NF037272.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HIR1415167.1"
                     /translation="MKKKKFPVFILCSLLTLTNIQTPWAFSDEAPDDPVFSDESVDDP
                     VFPVELPDDPAFSDEITPDEATYAKDTADISASGNSIPITADSGFADPVFQKWISENI
                     DTDRNGLLSDEEISMCTEISIPSMSVDSLEGIEYFYNLKTLDCSDNELLFLDVSANTV
                     LKSLNCSHNNLLSLDLSSCKKLKDLDISFNNCGSNSSISLPKSFSVEKLNISSVVLDD
                     FDFSRFSRLKELDCSNCNLRALKPASMPSLEKLICSGNLLDTLNLSRLSGLKYLDCSS
                     NSIQTLKLPDTRSLNTLYCQNNLLTKLNLSGFLSLKKLNCSDNKLLEPDLRGCFPEEL
                     ICNDNVIRTPMEFYDLSQFASPEDITIMENGMIDTSYRLTAINKLNPVKIRRRFTSAS
                     TEQYGTQIIYIGYLLENDFRSPALFQQMQQNYPLTDDHMLSREYLKEVSSLSFDEDFP
                     LFSSDLAYFTGLKSLRCTDLPQITELDLSRNPELTELICIGTGIRSLNLTSNKSLESV
                     QLCNNSLSSLNITGLTSLSSLDCRFNSLVSVDTTGCTALKDNLFFPQHTCTVNADQAG
                     KISFDQLGNFHQQLDTPAAILAGDELLYTPGDDFFTLLSPAVTMTEASFSIMDTNTWQ
                     ILGTCRMTINIESGTPVTPEKPSLKKIKSTHEVTITWKKAKNVSGYRILRREKNKSEA
                     SWKAIATVSSSRTSYTDSTGLIKKSYLYTVQSYTFRNGKKIYSKYNTKGLTGTAKLKR
                     PAVKKPQKDVVINLMWDEVPGADGYRIYRRRKGISKWTKLGDIEFSGLYYTDETAVPK
                     TVYDYAVRPYRKLGSKISLGDYSSTGYVCQTAIPKVSLTSVTEQDGGVTVKWKHQTYV
                     TGYFIYRADDQNSKYCKISDTPNGPDSEEYYAYYEPVPFGSVNPLYKVRAYVLIGKKY
                     YYGPYSRALALNPYSDNLNAHDFSEFHMGYQLSNSSSSTGFDRNYNDGGSFSMAAAYL
                     TRGSGPVYEWQAPYENISSASYDSFTPALRVNEIMFIPQRKNALDNQAIKQAIMNYGA
                     VSASYLSVDEYYSNDQISFCLPDDYDAGRAPAGSAPVISTQHAIAIVGWDDDYAKENF
                     PVRPAGNGAFLCKNSWGKDFGDNGYFYISYYDGFLGMQEFSMAFGKISSDNTCNRIYQ
                     YDPLGATTAFGYNDELYCANVFPENGQKLARKEQLGSVSFYTYDNDYNYEVYIVSSFK
                     NQNSFQKLPSPAARGNCRYAGYHTVDFSRPVTLKAGTRFAVVIKLWSSTGAKTYFEAP
                     LNGTSSRATASDGESYISHHGDIWTDFNTYLPNTNVCIKAFTNGKITENVPAAGSDGK
                     DISCESYSAEELKERGFLLNPAYEDGSSDMVSSVSTGNASSASAPVLPAAYDLRQSGR
                     VSPVKNQGEQGLCWTFSTYASLESFLLFS"
     gene            complement(4448..5632)
                     /gene="fabV"
                     /locus_tag="ACZPD3_03835"
     CDS             complement(4448..5632)
                     /gene="fabV"
                     /locus_tag="ACZPD3_03835"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HIR1415168.1"
                     /translation="MIIEPKVREYICTTAHPQGCAESVRNQADYACKQGMVNGTKKAL
                     IIGCSTGYGLASRICALENCGADTLGIMFERQANGRRTATPGWYNTAEFHRLASGKGV
                     YAKTINGDAFSKEIKEKAIELIKQDLGKVDLVVYSLAAPRRTDAEGKTWSSCLKTTDE
                     PFTEKSLDLRNNEITEKTVEPATEEEVLSTVKVMGGEDWADWIDALKAADVLTENAVT
                     VAYSYIGPELTYPIYYHGTIGTAKQHLQKTMSEINQAHPDVRAVISVNKGLVTQASAA
                     IPVVPLYFAILYKVMKRAGNHENCIQQIARLFTQKLYTPEGFRTDENGFIRMDDYELL
                     PEIQEEVKKCWKAVTTDTVNQYCDIDGYWEDFYHMFGFHYSDIDYTQDVDANVEINGI
                     VM"
BASE COUNT         1583 a         1401 c         1106 g         1687 t
ORIGIN      
        1 taacttctaa ccaatagtta atagacatct ccttgttcca actgtatact ggatataaag
       61 attatatttg tggccagaat ctattctgtc ccggatcact gtatcagaaa atccatatac
      121 acccaaatat tcaaaacaag gagggcttac ataatgaaaa agaaaaaatt tccggttttt
      181 attctatgct cacttctaac ccttaccaat attcagacac catgggcatt ttcggatgaa
      241 gcacccgatg atccggtatt ttcggatgaa tcggtcgatg atccggtatt tccggttgaa
      301 ctgcccgacg atccggcatt ttctgacgaa atcactcctg atgaagcaac atacgccaaa
      361 gacactgcag atatatctgc ttccggaaat tccattccga tcactgctga cagtggattt
      421 gcagaccctg ttttccaaaa atggatttcc gaaaacattg atacagatcg caacgggctt
      481 ctctctgacg aggaaatctc catgtgcaca gaaatcagta ttccttccat gtcagtcgat
      541 tccctggaag gcatcgaata tttttataat ctgaaaaccc tggactgttc tgacaatgaa
      601 ctgctgtttc tcgatgtatc agccaacaca gttctgaaat ctctgaactg ctcgcacaac
      661 aatctgctca gtctggatct ctcctcatgc aaaaaactga aagatcttga catctccttt
      721 aacaactgtg ggagcaacag ttccatcagt cttcccaaaa gtttttctgt ggagaaatta
      781 aacatctctt ctgttgttct tgatgatttt gatttctccc gtttttccag actgaaagaa
      841 ctggattgca gcaactgtaa tctgcgcgct cttaaacctg catctatgcc atcactggaa
      901 aagcttatct gttccggcaa tctcctggat accctgaact taagccgtct ttccggtttg
      961 aaatatctgg actgttcttc caacagtata cagactctga aactgccgga caccagatca
     1021 ctgaataccc tttactgcca gaataatctt ctcactaaac tgaacctctc cggtttttta
     1081 tcactgaaga aactgaactg ttccgacaat aagctgttgg agcctgatct tcgcggctgc
     1141 tttcccgagg aactgatctg taatgataac gtgatccgca ctcctatgga attttatgat
     1201 ctgtctcagt tcgcttcacc tgaagatatc accataatgg aaaatggcat gatcgacacc
     1261 tcttaccggc tgactgctat taataaatta aaccctgtaa aaatccgccg cagatttacc
     1321 agtgccagca cagaacaata tggcacacag atcatttaca ttggatatct gcttgaaaat
     1381 gatttccgat cacctgcatt gtttcagcaa atgcagcaga actatccact gacagacgat
     1441 catatgcttt cccgcgaata tctgaaggaa gtttcttccc tttcctttga cgaagatttt
     1501 ccgttatttt cttcagatct ggcctatttt acaggactga aaagtctgag atgtacggat
     1561 cttcctcaaa tcaccgaact ggatctcagc agaaatccgg aacttaccga actgatctgt
     1621 attggaacag gtatccgttc tctgaacctt acctccaaca aaagcctgga atctgttcag
     1681 ctgtgtaaca acagcctttc ctcattaaat ataaccggac ttacgtcact ttccagcctt
     1741 gactgccgct ttaattctct ggtcagcgtt gataccacag gctgcactgc tttgaaggat
     1801 aacctgttct ttccgcagca tacatgcacc gtcaatgctg atcaggcagg aaaaatttcg
     1861 ttcgaccaac tgggaaattt ccatcaacag ctggatacac cggcagcaat cctcgcaggc
     1921 gatgaacttc tgtacacacc cggagatgac ttctttactc ttttaagtcc tgccgtaacc
     1981 atgacggaag catctttttc cattatggat accaatacat ggcagattct cggaacctgc
     2041 cggatgacca taaacattga atcaggaact cctgtcactc ctgaaaaacc gtcattaaaa
     2101 aaaatcaaat ccactcatga ggttaccatc acctggaaaa aagcaaagaa tgtcagtgga
     2161 taccggattc tccgcagaga aaagaataaa tcagaggctt catggaaagc aatcgcaaca
     2221 gtttcctctt cccgtacctc ctatacagac agtaccggac tgatcaagaa atcctacctg
     2281 tatacagtgc agtcctacac tttccggaac ggtaagaaaa tctacagtaa atataacacc
     2341 aaaggcctga ccggtacagc aaaactcaaa cgccctgcgg ttaaaaagcc ccaaaaagat
     2401 gttgtcataa atcttatgtg ggacgaagtt ccgggtgccg atggctatcg catctaccgc
     2461 cgcaggaaag gtatctccaa atggacaaaa ctgggagata ttgaattttc cgggttgtat
     2521 tacacagacg aaactgctgt tcccaaaaca gtctatgatt atgcagtccg cccataccgt
     2581 aagcttggca gcaaaatttc tctgggtgat tacagctcca ccggatacgt ctgccagact
     2641 gcaattccaa aggtcagcct gacttctgtc actgagcagg atggcggcgt cacagtaaaa
     2701 tggaaacacc agacttatgt caccggctac tttatttatc gtgcagacga tcagaacagt
     2761 aaatactgta aaatttcaga tacaccaaac ggtcctgatt cagaggaata ctatgcatat
     2821 tatgaaccag tgccattcgg ctccgtaaat ccattatata aagttcgggc atacgtcctt
     2881 atcggtaaga aatattacta tggaccatat tccagagcac tggctctaaa tccgtactcc
     2941 gataatctca atgctcacga tttttcggaa ttccacatgg gttaccagtt aagcaacagt
     3001 tcttcctcca caggttttga ccgtaactat aatgacggtg gcagtttttc catggctgcc
     3061 gcctatctta cacgcggatc cggtcctgta tacgaatggc aggctcctta cgaaaatatc
     3121 tcatcagcat cctatgactc attcacacct gcgcttagag taaatgagat tatgtttatt
     3181 ccccagcgaa agaatgcatt agataatcag gcaatcaaac aggcaattat gaattacggt
     3241 gcagtatctg catcctatct ctccgttgac gaatactact ccaatgatca gatttccttc
     3301 tgccttcccg acgattacga tgcaggaagg gctcctgccg gttctgcacc tgtcatatcc
     3361 acacaacatg ctattgctat tgtaggatgg gatgatgatt acgcaaaaga aaactttccg
     3421 gtccggccgg ccggcaacgg tgcctttctt tgtaaaaaca gctggggaaa agatttcggg
     3481 gacaatggat atttttatat ttcctattac gacggctttc tgggtatgca ggagttctcc
     3541 atggcattcg gaaagatttc ctctgataac acctgtaacc gcatttatca gtatgatcct
     3601 ttaggtgcca ccactgcttt cggttataat gatgaactgt actgtgccaa tgtattccct
     3661 gaaaacggac agaaattagc caggaaagag cagcttggtt ctgtctcctt ctatacctat
     3721 gataatgatt ataactatga agtttatatt gtttcttcct ttaagaatca aaactccttt
     3781 caaaagcttc cttctcctgc tgcccgggga aactgccggt atgccggata tcatacggta
     3841 gatttttcca gacctgtgac tttaaaagca ggcacacgtt ttgctgtagt cataaagtta
     3901 tggagcagca caggtgcaaa aacgtatttc gaggctcctc tgaacggcac cagttccaga
     3961 gccactgctt ctgacggaga aagttatatc agccatcatg gggatatatg gacggatttt
     4021 aacacctatc tgccaaacac aaatgtgtgc atcaaagcat ttaccaatgg taaaatcaca
     4081 gaaaatgtcc ctgctgccgg ttcagacggt aaagatattt cctgtgaaag ctactccgct
     4141 gaggaactga aagaacgagg atttctcctc aatccggctt atgaagacgg aagttcagat
     4201 atggtctctt ctgtaagcac cggaaacgca tcttcagcct ctgcccctgt acttcctgcc
     4261 gcctatgacc tgcgtcagag tggtcgtgta tcccctgtga aaaatcaggg ggaacaggga
     4321 ttatgctgga cattctccac ttacgcttcg cttgagagct ttcttctttt ttcctgaaaa
     4381 aaatcctctc ccggaatatc tatgaacatt cataaatatt tccgggagag gattttttat
     4441 agtcatttta cattacaatc ccgttgatct ccacattcgc atctacgtcc tgtgtatagt
     4501 caatatcact gtaatggaat ccgaacatat gatagaaatc ttcccagtag ccgtcaatgt
     4561 cgcaatactg atttactgta tctgtagtga ctgctttcca gcatttcttt acttcttcct
     4621 gaatctcagg gagcagttcg taatcatcca tacggataaa tccgttttca tctgtccgga
     4681 aaccttccgg ggtatagagt ttctgtgtaa aaagccttgc aatctgctga atacagtttt
     4741 catgatttcc ggcccttttc ataactttat aaagaatagc aaaatacagc ggtacaaccg
     4801 gaatagctgc acttgcctga gttaccagac ctttgtttac agaaattact gcgcggacat
     4861 ccggatgtgc ctgattaatc tctgacattg ttttctgtaa gtgctgtttg gcagttccaa
     4921 tggttccatg atagtagatt ggataagtca gttccggtcc gatgtaggaa tatgctacgg
     4981 ttactgcatt ctcagtcagt acatctgctg ctttcagcgc atcaatccag tctgcccagt
     5041 cttcaccgcc cataaccttt acagtactta aaacttcttc ctctgtagca ggttctactg
     5101 ttttctctgt gatctcatta tttctcagat ccagactctt ctctgtaaat ggctcatctg
     5161 tagttttcag acaggaagac caggtctttc cttctgcatc tgtacgtctc ggtgctgcca
     5221 ggctgtagac taccaggtcc acttttccaa gatcctgttt gatcagttca atcgccttct
     5281 ccttgatttc ctttgaaaaa gcatctccgt taatggtctt tgcataaaca cctttcccgg
     5341 aagccagtct gtgaaactct gctgtattat accatccggg tgtagctgtc cttctgccat
     5401 ttgcctgacg ttcaaacatg attccaagag tatcagcacc acagttttcc agtgcacaga
     5461 tacgggatgc gaggccgtag cctgtagaac atccgatgat cagtgctttt tttgttccgt
     5521 ttaccattcc ctgtttacag gcgtaatctg cctggttgcg cacactctct gcacatccct
     5581 gcggatgtgc ggttgtacag atatattctc ttacttttgg ctcgatgatc ataacttttc
     5641 tccctaaatt tatttctttg cctgatattt tttagtcaag cggataaaat catccgcttc
     5701 gttacttgtt cataatcgaa taactgctcc gcagtatata cgcttgaatt gtatgtattg
     5761 caaatatttt gaatctc
//
LOCUS       DBMDXG010000088         5772 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3496_length_5772_cov_2.150238, whole genome shotgun sequence.
ACCESSION   DBMDXG010000088 DBMDXG010000000
VERSION     DBMDXG010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3496_length_5772_cov_2.150238"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            154..672
                     /locus_tag="ACZPD3_03840"
     CDS             154..672
                     /locus_tag="ACZPD3_03840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1415169.1"
                     /translation="MSTNNMDRFEFRCIRPEETQQAIEIEQICFPPNEACSPKSLTER
                     IKATAETFLVAEDKETGKLAAFLNGVPTDEEAFRDEFFTDISLSNPEGKNIMLLGLDV
                     RPEYRMQGLGRELVSRYCQREAQKGRKKLFLTCLDEKVKMYEKFGFTDLGQANSTWGG
                     EAWHAMSIEIER"
     gene            704..1402
                     /locus_tag="ACZPD3_03845"
     CDS             704..1402
                     /locus_tag="ACZPD3_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1415170.1"
                     /translation="MSLYAIGDFHLSFTVNKPMDVFDKRWKNHVVKIEKYWKRKVTEN
                     DTVVITGDHSWGRDLAECQADLDFIMALPGRKILLRGNHDMFWDAKKTENLNEMFRGK
                     LEFLQNNFYTYEDYALVGTKGYCYEGKDSYEHFLKIRERELGRLRCSFEGAKAAGYEK
                     FIMFLHYPPTSIGEMESPFTLMAQEFGAEKVIYSHCHGEKRYDDSFKGYVDGIEYKLV
                     SGDYLNFKPELVLR"
     gene            1499..2584
                     /locus_tag="ACZPD3_03850"
     CDS             1499..2584
                     /locus_tag="ACZPD3_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1415171.1"
                     /translation="MKIYYREKSGGIEILRCFGIEGRVEIPGMIDDKLVISAAPYAFS
                     SHMDEKEDLKNASLWEVSDGLEFGREEHVLAGNDVEEIVFPYTLKEIGRYIFYGCGNL
                     KKLEFSDSLMQIGCGAFTGCHALEKLTVHMEQGKKSGVKEMLGEMWQRIDVNFLYEYE
                     EARLVFPEHYDEAVENTPARILYTEYHGSGSNYRQCFYDKELNYQEYDRLFEMAVAMD
                     KLEVLVDMSFGRLEFPYELTGKARENYREYIRKNLGDIAEYLVKQEDMHRLEVISSQK
                     LWTLGGIDSALDCASKRKETEVSAFLMNERANLVDNTAGSERIDVDKLQNSQEADRTE
                     QGKNEQSQTTEKSLNTRTILRKKRFEL"
     gene            2606..3973
                     /locus_tag="ACZPD3_03855"
     CDS             2606..3973
                     /locus_tag="ACZPD3_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR1415172.1"
                     /translation="MQPEDKIQEIAERAERLQEIGSSILRAARDELYLGMRFLDVALS
                     SFSYQMDGQVHGFGTDGRVMYFQPQMLGGLYRENRILVNRGYLHMVFHCIFRHFAWSG
                     TEGKKRADDGITIQERMRDLSCDIAVEHMIDGMNYRSIRFSRSLLRRETYRLLEKEGK
                     TLNAQRVYKILSEWNLNEKDLTNLEQEFRTDDHRYWESKKPDQKPNPMLSRKWGEIND
                     GIETDLETFSQEAGERDGDFLEQIKTENRSRYDYREFLRKFAVFHEELAVDDDSFDYN
                     FYTYGLRLYGNMPLIEPLESKEVKKIEEFVIVIDTSMSCSGELVRKFLEETYGVLSES
                     ESFFTKINVHIIQCDEKVHTDKRITSRKEMKDYMEHLELYGDGGTDFRPAFEWVDKLL
                     EQHEFHNLKGMIYFTDGFGIYPKKMPPYKTAFVFMQDNYRDVDVPVWAMKLILDEDDF
                     EKPDR"
     gene            4029..5582
                     /locus_tag="ACZPD3_03860"
     CDS             4029..5582
                     /locus_tag="ACZPD3_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1415173.1"
                     /translation="MNIKRAKQEIKDSIEAYLAKDEFGEYLIPAIRQRPILLMGAPGI
                     GKTQIMEQIARECKVGLVSYTITHHTRQSAVGLPFIKEKTFGQETFSVTEYTMSEIIA
                     SVYEKMEKTGLKEGILFIDEINCVSETLAPMMLQFLQGKTFGNQKVPEGWVIVTAGNP
                     PEYNKSVREFDVVTLDRIKRIDVQPDFEVWKEYAYEQGIHPAVISYLELRRKNFYRME
                     NTVDGRIFATARGWEDLSRLIQVYETLDKEVDREVVYQYIQHPMIAKDFAAYLALCNK
                     YKTDYAVEDLLQGKWTPITLGKIRNASLDEHLSIVGLLNGKLSQLFADCYFMDAYVTK
                     LYGYMTEYRDNLPEMTLESIYKKAENDFQTTKKSELLTKNEEKVFIRTVDFLEKLWIE
                     LRGETGSEDKTENNKAVEISEKDTYERAKTAFATEADSLETQTEYISQTLQNVFDFME
                     AAFGDSQEMVAFITELNANFYSIWFIRENGSDQYYRHNKGLLFDDRQKLILGQMEELE
                     NTMKRGLKN"
BASE COUNT         1897 a          905 c         1527 g         1443 t
ORIGIN      
        1 acattaacat ggagtttaat gcacactacg tacgggaaga tttggatgag aaatcaaaga
       61 gaagctgccc gatgtagata aaacaactat atgggaaact tacagatctc gaatatacag
      121 gaaataacaa gatagcagga gaaggataat aaaatgagta caaacaatat ggatagattt
      181 gaatttagat gtatcagacc ggaggagaca cagcaggcaa tagaaatcga gcagatctgt
      241 ttcccgccaa atgaggcatg ttccccgaaa agtctgacag agagaataaa agcaacagca
      301 gagacatttc tggtagcgga ggataaggaa accggaaagc tggctgcatt tcttaatgga
      361 gttcctacag atgaggaggc tttcagagat gaattcttca cagatatttc tctgagtaat
      421 cctgaaggca aaaatatcat gcttctgggt ctggacgtcc gtccggaata tcgtatgcag
      481 ggcctgggcc gtgaactggt atccagatac tgccagaggg aagctcaaaa aggacgaaag
      541 aaacttttcc tcacctgtct ggatgaaaaa gtgaagatgt atgagaaatt cggctttaca
      601 gatctgggcc aggcaaattc cacctggggc ggagaagctt ggcatgccat gagcatcgag
      661 attgaaagat aattaacaat cagaacagac aggagagtgt acaatgagcc tatacgcaat
      721 cggtgatttt catctttcgt ttacagtcaa taaacctatg gatgtatttg ataaaagatg
      781 gaagaatcat gtagttaaga ttgagaaata ctggaagaga aaagtaacag agaatgatac
      841 agtagtgatt accggagatc attcctgggg gagagatctt gcagagtgtc aggcggatct
      901 ggattttatt atggctcttc cgggcaggaa aatcctgctt cggggaaatc acgatatgtt
      961 ctgggatgca aagaaaacag aaaatctgaa tgagatgttc agaggaaagc tggaattttt
     1021 gcagaataat ttttatacat atgaggatta tgcactggtg ggaaccaagg gctactgtta
     1081 tgaaggaaaa gacagttatg agcatttcct gaagatccga gagcgggaac tggggcggct
     1141 gcgctgctct tttgaagggg cgaaggcagc aggatatgag aagtttatca tgtttcttca
     1201 ttatccgcct acaagtattg gagagatgga gagtccgttt accctgatgg cacaggaatt
     1261 tggcgccgag aaggtgatct attctcactg tcatggagag aagcgatatg atgacagctt
     1321 taaaggctat gtggatggaa tagagtacaa gctggtgtca ggagactatc tgaattttaa
     1381 accggagctg gttttacgct aattatattg tctggaaaaa atccctggga agtattatac
     1441 ttttcaggga tgattttatg tgctttgaga gaaaatatct tcggggagaa acgagaaaat
     1501 gaagatttac tacagagaga aatctggcgg gattgaaatt ctgcggtgtt ttggaataga
     1561 gggcagagtt gagattccgg gaatgataga tgacaaattg gtaattagtg cagcaccgta
     1621 tgcattttct agccatatgg atgagaagga agacctgaaa aatgccagtc tgtgggaagt
     1681 gtcagatggt cttgagtttg gcagagaaga gcatgttctg gcaggaaatg acgtggagga
     1741 gattgttttt ccttacactt taaaagagat tggacgatat attttttatg gctgcgggaa
     1801 tctgaaaaag ctggagtttt ctgacagcct gatgcagata ggctgtggtg catttacagg
     1861 ctgccatgca ttggagaagc tgactgtgca tatggagcag ggaaagaagt ctggtgttaa
     1921 ggaaatgctg ggcgagatgt ggcagaggat agatgtgaac tttctttatg agtatgaaga
     1981 agccagactt gtatttccgg agcattatga tgaggcggtt gagaacactc cggcgcgtat
     2041 tttgtatacg gaatatcatg gatcaggcag taattacagg cagtgttttt atgataagga
     2101 gctgaattat caggaatatg acaggctttt tgaaatggcg gtagccatgg ataagctgga
     2161 agttctggtt gatatgtcat ttggcagatt ggagtttcct tatgaactga ccgggaaagc
     2221 cagggagaac tacagggaat atatccgtaa gaatctggga gatattgcag aatatctggt
     2281 aaagcaggag gatatgcaca gattggaagt gatatcctcg caaaaactct ggacactggg
     2341 aggaattgat tctgcgttgg attgtgcatc taagagaaaa gaaacggaag tatccgcatt
     2401 tttgatgaat gaaagagcaa atctggtgga taatacggca ggaagtgaac gtatagatgt
     2461 ggataagctg caaaatagtc aggaagctga caggacagag caggggaaaa atgaacaatc
     2521 acaaactaca gaaaaatctt tgaatacaag aactatacta agaaaaaaga ggtttgaact
     2581 gtgatagata tagaggaaaa taatgatgca gccagaggat aaaatacagg aaatagcaga
     2641 aagagcagag cgtttgcagg aaatcggcag cagtattctt agggctgcgc gggatgaatt
     2701 atatctggga atgcggtttt tggatgtggc gctgagcagt ttttcttatc agatggatgg
     2761 tcaggtccat ggatttggaa cggatggaag agtgatgtat ttccagccgc agatgctggg
     2821 aggcttatat agagagaata ggattctggt gaacagaggg tatctgcata tggtattcca
     2881 ctgtattttc cggcattttg catggagcgg tacagaaggt aagaagaggg cagacgatgg
     2941 aatcacaatc caggaacgta tgcgtgatct gagttgtgac attgcagtgg agcatatgat
     3001 agatgggatg aactatcgga gtatccgttt ctcacgcagt cttctgcgaa gggaaactta
     3061 ccgtctcctt gaaaaagagg gaaaaaccct caacgcacag agagtatata aaatattgtc
     3121 agaatggaat ctgaacgaaa aggatcttac aaatctggaa caggaatttc gcacagatga
     3181 ccacagatac tgggaaagta agaaaccgga tcagaaaccg aatcccatgc tcagccgcaa
     3241 atggggggag atcaatgatg gaatcgagac ggatctggaa accttttccc aggaagcagg
     3301 tgagcgggac ggagatttcc tggagcagat aaaaacagag aacagaagca ggtatgatta
     3361 cagagaattc ctgcggaaat ttgcagtttt tcatgaagaa ctggcagtgg atgatgacag
     3421 ttttgattac aatttctata cctatggtct gcgtctctac ggaaacatgc cgctgatcga
     3481 accactggaa tctaaagaag ttaagaagat agaggaattt gtgatcgtca tcgatacttc
     3541 catgtcctgt tcaggagaac tggtgagaaa gtttctggag gaaacctacg gtgtacttag
     3601 tgagagtgag agctttttta caaaaataaa tgtgcacatc attcagtgtg atgagaaagt
     3661 ccacacagat aaaaggatca cctcccggaa agaaatgaaa gactatatgg aacatctgga
     3721 actgtatgga gatggcggaa cagacttcag gcctgcattt gaatgggtgg acaaactgct
     3781 ggaacagcat gaatttcata acctgaaagg gatgatctat tttacagacg gattcggaat
     3841 ttacccgaag aaaatgccgc cgtataagac tgcatttgtg tttatgcagg ataattacag
     3901 agatgtggat gtgccggtat gggccatgaa actgattttg gatgaggatg attttgagaa
     3961 accagacaga taatgaaata aaaaactgag taaaagtaag aagtgttcag caggaaagga
     4021 tttatattat gaatataaag cgagcaaaac aggagataaa ggactcaata gaagcatacc
     4081 ttgcgaagga tgaatttgga gaatatctga tcccggccat acgccagaga ccaattctcc
     4141 ttatgggggc accgggtatt ggaaagacgc agatcatgga acagatcgcc agggaatgta
     4201 aggtaggtct ggtatcttat actatcaccc atcacacccg tcagagtgca gtaggactgc
     4261 cgtttatcaa agagaaaacc tttggtcagg agactttttc tgtcacagaa tatactatga
     4321 gtgagattat tgcctctgtc tatgaaaaga tggagaagac aggcctgaaa gaagggattc
     4381 tttttattga tgagatcaac tgtgtatcag agaccctggc gcctatgatg ctgcagtttc
     4441 ttcagggaaa gacctttggc aatcagaaag taccggaggg atgggtgatc gtgacagcag
     4501 gaaacccgcc ggaatacaat aaatctgtcc gtgaatttga tgtggtaact cttgacagaa
     4561 tcaagaggat tgatgtgcag ccagactttg aagtatggaa agaatatgcc tatgagcagg
     4621 ggatccaccc tgctgtgatc tcttatctgg aacttcgcag aaagaatttc tacagaatgg
     4681 agaacacagt agacggacgg atttttgcca ctgccagagg ctgggaagat ctttctaggc
     4741 tgatacaggt atatgaaaca ctggataaag aagtggacag agaggtggta tatcagtata
     4801 tccagcatcc aatgatcgca aaagattttg ccgcatatct ggcattatgc aataagtata
     4861 agacagacta tgcagtggag gatctgcttc agggtaaatg gacaccgatc actctgggta
     4921 agatccgcaa tgcttcactg gatgagcatc tgagtattgt tggtctgctg aacggaaagc
     4981 tcagccagtt atttgcagac tgttatttta tggatgccta tgtgacaaag ctctatggtt
     5041 acatgacaga atacagagat aaccttccgg aaatgacgct ggaatccatt tataaaaagg
     5101 cagaaaatga ttttcagact acaaaaaaat ctgagcttct cactaaaaat gaagaaaaag
     5161 tatttatccg tacagtggat tttcttgaga aattatggat agaactgagg ggagaaactg
     5221 gttctgaaga taaaacagag aataataaag ctgttgaaat ttcagaaaaa gatacctatg
     5281 agcgggcaaa aacagctttt gcaacggagg ctgacagtct ggaaactcag acagaatata
     5341 tctctcagac gcttcagaat gtttttgatt ttatggaagc tgcctttggt gatagccagg
     5401 aaatggtagc atttattacg gaactgaatg caaactttta cagtatctgg tttatccggg
     5461 aaaatggttc tgaccagtat taccgtcaca acaagggact actgtttgat gacagacaga
     5521 aactgatcct gggacaaatg gaggaactgg agaacacgat gaagcgggga ctaaaaaatt
     5581 aatttttatg ccaattagca ttggattttc agataaatcg tggtatgctg atgaatagaa
     5641 ttatgatttg agtgaaaaat atcaggaatg caggcgcatt tgactgtgca gcagtagaac
     5701 gatatggaat cactagaaaa aacgaggagg aatataaaat ggaattagaa gtattaagaa
     5761 aagatatggt tg
//
LOCUS       DBMDXG010000089         5770 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3500_length_5770_cov_1.653731, whole genome shotgun sequence.
ACCESSION   DBMDXG010000089 DBMDXG010000000
VERSION     DBMDXG010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3500_length_5770_cov_1.653731"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            328..507
                     /locus_tag="ACZPD3_03865"
     CDS             328..507
                     /locus_tag="ACZPD3_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIR1415174.1"
                     /translation="MTNKNFMTAQDVADALGVSKSYAYKLVRQLNDEMEALGYITVRG
                     RVDANYFRKKVCYSE"
     gene            526..1611
                     /locus_tag="ACZPD3_03870"
     CDS             526..1611
                     /locus_tag="ACZPD3_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR1415175.1"
                     /translation="MAVYKDNATGTWRVVFRYTDFTGERKQTQKRGFQTKRDALAWER
                     EQLLKQDSKLDMTFASFYEIYAEDKKARLKESTWESKSHVIEKKILPYFGKRKIADIE
                     AKDVIAWQNELMKYRDEKGKPYSGDYLKTIHAQLTAIFNHAVNFYNLPYNPAKRAGCM
                     KVQTSKEVEFWTKEEYLKFAEVMMDKPRSYYAFEMLYWTGIRSGELLALCPSDFDFEK
                     QTVKISKTYHRSKGRDIITTPKTAKSNRTIKMPKFLCEEIQEYMKMLYEVKPDDRIFT
                     VTKSYLDHEMRRGVKEAGLKKIKVHALRHSHVSLLIHMGFTVLAIAERMGHEAEKITL
                     KYAHLFPTVQTEMAEKLDMERMKKEDE"
     gene            1612..1935
                     /locus_tag="ACZPD3_03875"
     CDS             1612..1935
                     /locus_tag="ACZPD3_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIR1415176.1"
                     /translation="MDRKLDNRGRWRNKTVAFRVSEEEAKQIDVCVRLSGLTKQDYIV
                     RRLMCRDIVVQGNPRVYKALKNQMAELYEELKRIEQFSEENEELLYTLQLVAETLNGM
                     KEDED"
     gene            1932..3095
                     /locus_tag="ACZPD3_03880"
     CDS             1932..3095
                     /locus_tag="ACZPD3_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1415177.1"
                     /translation="MMTKEKMTAPVASVGADGEQPLNNKITNQSIADLPVKGNLQATN
                     NAENVAKSANKKNPDELETVSMMELYDTAYPPKLPIVDGLLYNGTYLFVGSPKIGKSF
                     FMARIGYHISKGLPLWGFPVRQGTVLYLALEDDYARLQKRLSQMFGMEGSENFYFATK
                     SKSLNAGLEQQLVQFVTEHKDARLIIIDTLQKVREVGGDKFSYASDYEIVTKLKAFSD
                     HYGICFLVVHHTRKMESSDSFDMISGTNGLLGAADGAFVMQKEKRTDNKAVLEVAGRD
                     QQDQKLWLNFNRERCVWELTKTETELWKEPEDPLLEKIKELITAEQPEWTGTATELTE
                     FLQVEMQPNALSRKLNVSLERLLIEYGIQYRSERGHEGRRIYLTYMGSQRDDM"
     gene            3181..3414
                     /locus_tag="ACZPD3_03885"
     CDS             3181..3414
                     /locus_tag="ACZPD3_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complexin-2"
                     /protein_id="HIR1415178.1"
                     /translation="MKNVQISQELFMQLLRFHLMEDVGCESEIKQELEKKLDKMVMRD
                     LYGKFKTAPTEEERERARKEYLNRRGVPESFRW"
     gene            3499..3726
                     /locus_tag="ACZPD3_03890"
     CDS             3499..3726
                     /locus_tag="ACZPD3_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008373529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HIR1415179.1"
                     /translation="MREGRLGYNCENGRYGLLSMDLWVDTGFHCGECMEVLVDDKWVK
                     TRMEMNPAMEWYLVGTPYCGDLEYVRARIPE"
     gene            4042..5589
                     /locus_tag="ACZPD3_03895"
     CDS             4042..5589
                     /locus_tag="ACZPD3_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HIR1415180.1"
                     /translation="MTKLSNVKGRITYISSHAKQENLYAVYETTERKFWRELAKCNQE
                     EFVKSGTEGKCIEARELIIALPESFVEYQPDMLLKLFTEHFKQNYGTECISALHHNKR
                     KTNYHIHLIFSERKLLDEPIIKIASRNRFYDKNGKHVRTKKEILGEDGQIREGCYIVK
                     KGEVYEKRIFTVKDEYFKSNAFLEEVKHSYTDLMNLYVKDDKQKLQVFERGGIYLATK
                     KIGKNNPKAQEIESDNQKRQEWNRTVDVALISGVSEPHILEVKRKQISEPIKGAISQI
                     GRKPRLFAGFVTVAIAALELMIESVLMKKYKEVSVQKEEINQTDEDIMLEKTATQVSD
                     TENTQADAHESMPKAPEQEKPETKQSEMTRMASKYPRFFKVYNELEQQNTVIYKKEQQ
                     RTAKKKELSEVKGWFKGRKKKELQKEITELTSQIRDMKDYLPKIVQRVGYRNVQEFLK
                     DFQIAKSEYSQYQKVIAQWKQENGKEPEQQSHGVRAKLAANRKKIEQEQKNTQRTRSQ
                     SKDRGAR"
     gene            5617..>5770
                     /locus_tag="ACZPD3_03900"
     CDS             5617..>5770
                     /locus_tag="ACZPD3_03900"
                     /inference="COORDINATES: protein motif:HMM:NF025561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIR1415181.1"
                     /translation="MGENGISYSLGEDGLYYPDLYLPEETRYPIGKYGMMRRTYLKEH
                     RKSLYLE"
BASE COUNT         1999 a          988 c         1524 g         1259 t
ORIGIN      
        1 acttttctgt ttaagctgag tgtaccacag ataaaagaga atagcaagaa aacttaaact
       61 aaaaaattta agaatttttc tgcaaaccta ttgacttaac tcaaaaaagt tagtatagta
      121 cagatacaga aacttaaaca aaataagtta atacaattaa atgaccatcc gaactgatac
      181 cactggaaga acaggcgatg accaaaagcg agcgtgccga aggtggaaat acgacaaagt
      241 accgagcagg gcaacttgtc aggaacgggg gagggagaat ggcgaagaac aaagaaaatg
      301 aaaaacaaac gagaggagat gaagtgtatg acgaacaaga attttatgac agcccaggat
      361 gtagcagacg ctttaggcgt atcaaaatct tatgcctata agctggtcag acagttaaat
      421 gatgagatgg aagcacttgg ctatatcact gtcaggggca gagtggatgc gaattacttt
      481 cgtaaaaaag tatgttacag cgaataaggg aaaggaagtg gttgaatggc tgtatacaaa
      541 gataatgcca caggaacgtg gcgggtagtg ttccgttata ccgattttac cggggaacgg
      601 aaacagacac agaaaagagg gtttcagacg aagcgagatg cactggcgtg ggagcgagaa
      661 caattattaa agcaggattc caagctggat atgacatttg cgagcttcta tgaaatctat
      721 gcagaggata aaaaggcaag gctgaaggaa agcacatggg aatccaagag ccatgtgata
      781 gagaagaaaa ttcttcccta cttcggcaaa cgcaagattg cagacattga agcaaaggat
      841 gtaatcgcat ggcagaatga gttgatgaaa taccgggatg agaagggaaa accatattcc
      901 ggtgattatc tgaaaacgat tcatgcacag cttacagcaa tcttcaacca tgcggtcaat
      961 ttttacaatc tgccctataa tccggcgaag cgtgcaggtt gcatgaaagt acagacgagc
     1021 aaggaagtgg agttctggac gaaggaagaa tatctgaaat ttgcggaagt gatgatggat
     1081 aagccacgct cctactatgc atttgaaatg ctgtactgga caggtatccg ttccggcgag
     1141 ctgcttgccc tctgcccttc tgattttgat ttcgagaagc agaccgtaaa gatcagcaaa
     1201 acgtatcaca ggtcaaaggg cagggacatc attactacac caaagactgc gaaaagcaac
     1261 cggacaatca agatgccgaa gtttctctgc gaggagattc aggagtatat gaaaatgctg
     1321 tatgaagtga agccggatga ccgcattttt acggtgacaa aatcttatct ggatcatgag
     1381 atgcgtcgtg gcgtaaaaga agccggatta aagaagatta aggttcatgc actccgtcat
     1441 agtcatgttt ccctgttgat tcacatggga ttcactgtgc ttgccatagc agagcgtatg
     1501 gggcatgaag cagagaaaat cacattgaaa tatgcacatc tgtttccaac tgttcagacg
     1561 gaaatggctg aaaagctgga tatggaacgc atgaaaaagg aggatgagta gatggacaga
     1621 aaacttgaca acaggggcag atggagaaac aaaacggtag ctttccgggt atctgaggaa
     1681 gaagcaaagc agattgatgt gtgtgtccgg ctttcgggac tgaccaagca ggactatatt
     1741 gtgagaaggc tgatgtgccg ggacattgta gtgcagggca atccgagagt gtataaggca
     1801 ctgaaaaatc agatggcaga gctttatgag gagttgaaaa ggattgagca gttttcagag
     1861 gaaaatgagg aattgcttta taccttgcag ttagttgcag agacactgaa tggaatgaag
     1921 gaggatgagg attgatgaca aaagaaaaaa tgactgctcc ggtagcatct gttggcgcag
     1981 atggggaaca gccattaaat aataaaataa ccaaccaaag tatagcagat ttgcccgtta
     2041 agggcaatct gcaagccaca aataatgcag aaaatgtggc aaaatcagca aataaaaaga
     2101 atccggatga actggaaacc gtttccatga tggaattata tgatacagct tatccaccga
     2161 agctgccgat tgtggacgga cttttgtata acggaacgta tctctttgta ggttctccga
     2221 aaattgggaa atcattcttt atggcacgga tcgggtatca catcagcaaa ggacttccct
     2281 tatggggatt tccggtaaga cagggaactg tcctgtatct ggcactggaa gatgattatg
     2341 caaggttaca gaaacggtta tcgcagatgt ttggaatgga aggcagtgag aatttttatt
     2401 ttgctacaaa gtcaaaatca ttaaacgccg gactggagca gcaacttgta cagtttgtaa
     2461 cagagcataa ggatgcaagg ctcattatca ttgatacgct ccagaaagtc cgagaagttg
     2521 gcggagataa attcagttat gccagtgact atgagattgt gacgaagttg aaagcgttca
     2581 gtgaccatta tggcatctgc tttctggtgg tgcatcacac aaggaaaatg gaatcctcgg
     2641 acagctttga catgatttcc ggcacgaatg gactgctggg agctgcggat ggggcatttg
     2701 tcatgcagaa agaaaagcgg acagataaca aggcagtttt ggaagtagca ggacgtgacc
     2761 agcaggatca gaagctgtgg ctgaatttca accgggagag atgtgtctgg gagctgacca
     2821 agacagaaac agaactctgg aaagagccag aagatccact acttgaaaaa atcaaagaac
     2881 tgataacagc agaacagccg gaatggacgg gaacagcaac cgaactgaca gagtttctgc
     2941 aagtggagat gcagccgaat gctttgtcac gaaaactgaa tgtcagtctg gaacgtctgc
     3001 tgatagaata tggaatccag tatagatcag agcgtggaca tgagggcaga cgaatttatc
     3061 tgacctatat gggaagccag cgtgacgata tgtgacgatg gtgacggtaa tttatatagc
     3121 gtgccggtag tgaaaacatc gtcactatcg tcacgaccgt cacggaaagg atgggataag
     3181 atgaaaaatg tgcaaatatc acaggaatta ttcatgcagt tacttcggtt tcatttaatg
     3241 gaggatgtag gttgcgagag cgaaatcaag caggaactgg agaaaaaact ggacaagatg
     3301 gtaatgcgtg acctgtatgg aaagtttaaa actgctccga cagaggaaga acgggaacga
     3361 gcgagaaaag aatatctgaa caggcgagga gtgccggaga gttttcgttg gtaatttctt
     3421 ccttgatggt aagaacagga gcgtggcacg cccctgtata gagcggacaa ggagaaaact
     3481 ggaaaggaga agcacacaat gagagaagga agattaggtt acaactgtga aaatggcaga
     3541 tacggcttgc tgtccatgga tttgtgggta gatacaggat ttcactgtgg agagtgtatg
     3601 gaagttctcg tggatgataa gtgggtaaaa accagaatgg agatgaatcc tgcaatggaa
     3661 tggtatcttg tgggaacacc gtattgcggc gatttggagt atgtccgggc aagaatacca
     3721 gagtgattca gaatgaaaaa cagcccggat acgggcatat aaaaatgggc ggatacgccc
     3781 aagatgaaaa gcataagccc ggcagctacg gctgacaaaa gcacttacca aagccctcgg
     3841 cgtttgagag cgaaatacac ccatcgcaca aatctgacga tttatgctca gcgtatttcg
     3901 ccctgccggg agcttaaatg ccggatacgg caatgagttc gtaaacggat gcctaagcat
     3961 ccactcacaa aaaatatggc agaatttact ttgccgaaga aagaggaggg agtgcctatc
     4021 gtaagaaatt catttattca aatgacaaaa ttaagtaacg taaaaggtcg aatcacttat
     4081 atatccagtc atgcaaagca ggaaaatctc tatgctgttt atgagactac ggagcgaaaa
     4141 ttctggcggg aacttgccaa atgcaatcag gaagaatttg taaaaagcgg aacagaaggg
     4201 aaatgtatcg aagccagaga actgattatt gccttgccgg aaagtttcgt ggagtaccag
     4261 ccggatatgc tgctgaagct gtttacagag catttcaagc agaattatgg aacagagtgt
     4321 atatcggcat tgcaccacaa taaaaggaaa acgaattatc atattcattt gatcttttcg
     4381 gaacggaaat tattggatga accgatcatc aagattgcca gccggaatag gttctatgat
     4441 aaaaatggaa agcatgtccg tactaagaaa gagatacttg gggaagatgg tcagattcgg
     4501 gaaggttgct atattgtgaa gaagggcgag gtttacgaga agcgaatatt taccgttaag
     4561 gatgagtact ttaaaagcaa tgcgtttttg gaagaagtga agcattccta tacagatttg
     4621 atgaatctgt atgtgaagga cgataaacaa aagctgcaag tctttgagcg tggcggtatt
     4681 tatcttgcaa ctaagaaaat cggaaagaat aatcctaaag cacaagagat tgaatcagat
     4741 aatcagaaac gacaggagtg gaaccggaca gttgatgtgg cactaatcag tggtgtgtcg
     4801 gagccacata ttttggaagt gaaacgtaaa cagatttcgg agccgattaa gggtgctatt
     4861 tcacagattg ggagaaaacc aagattattt gcaggattcg ttacagtggc gattgctgca
     4921 ctggagctga tgattgagtc agttcttatg aaaaagtaca aggaagtgtc tgtccagaaa
     4981 gaagaaataa atcagacaga tgaagatata atgcttgaga aaacagcaac acaagtgtca
     5041 gatacagaaa atacccaagc agacgcacat gagtcgatgc caaaagcacc ggaacaggaa
     5101 aaaccagaga caaagcaatc ggaaatgaca aggatggcat cgaaataccc aagattcttc
     5161 aaggtttata atgaactgga acagcagaat acggtaatct acaagaaaga acagcagcgt
     5221 accgctaaga aaaaagaact gtcggaagtt aaaggctggt tcaagggcag aaagaaaaag
     5281 gaattacaga aagaaatcac agaactgaca agccagatta gagacatgaa agattatctt
     5341 ccgaagatcg tacagcgagt tggataccgg aatgtgcagg agtttttgaa agacttccag
     5401 attgcaaagt ctgagtacag ccagtatcag aaagtgatcg cacagtggaa acaggaaaac
     5461 ggaaaagagc cggaacagca gtcacatggt gtcagagcaa aactggcggc aaacagaaag
     5521 aaaatagagc aggaacagaa aaatacacag cgtacccgaa gccagagtaa agaccgaggg
     5581 gcaagatagg aggaagaatt catggagaaa cacatcatgg gagaaaatgg aatcagctac
     5641 tcgcttggag aggacggtct gtattatcca gacctgtacc tcccggagga gacgaggtat
     5701 ccaattggaa agtatggaat gatgcggaga acgtatctga aagagcatcg gaagagcctg
     5761 tatctggaac
//
LOCUS       DBMDXG010000090         5699 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3537_length_5699_cov_1.853162, whole genome shotgun sequence.
ACCESSION   DBMDXG010000090 DBMDXG010000000
VERSION     DBMDXG010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3537_length_5699_cov_1.853162"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="ACZPD3_03905"
     CDS             <1..1602
                     /locus_tag="ACZPD3_03905"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1415182.1"
                     /translation="AINTQALRLLRLFGNTTSKKVTPSVGAEQEYFLIDKEKWLQRKD
                     LIYTGRTLFGAMPPKGQEMDDHYFGSIRQRISAYMKEVNEECWKLGIPAKTQHNEVAP
                     AQHELAPIYAPVNIAADQNQMMMRILKKVASRHGMRCVLHEKPFAGVNGSGKHNNWSL
                     TTDDGINLLDPGKTPHENIQFLLVLTCILKAVDEHADLLRESAADVGNDQRLGGHEAP
                     PAVISVFLGEQLEDVLEQLVSTGTATHSKKGSKLETGVKTLPDFMKDATDRNRTSPFA
                     FTGNKFEFRMVGSRDSISACNVVLNTITAEVFKEVCDRLEKAPDFELAVHDLIKEYAT
                     DHQKIVFNGNGYAPEWEKEAKRRGLPILPSMVDAIPALTTEKAVKLFESFDVFSRAEL
                     ESRAEIKYEIYSKAINIEAKTMIDMATKQIIPAVIRYTTVLGESINTVKTAGEGLIDV
                     SVQLDLLKKASKLLKAANSAMSKLSKLVEKTQEYAEGRDRAVFCREKVVPAMEALREP
                     VDELEMIVDKEMWPMPSYGDLVFEV"
     gene            1719..2639
                     /locus_tag="ACZPD3_03910"
     CDS             1719..2639
                     /locus_tag="ACZPD3_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1415183.1"
                     /translation="MEELKFLEEQQVAPELIKRVEEFRSRYEVVPETADRIVKPSIPF
                     YGKDILEMAIAGLLKGENLLLTGPKATGKNILAENLAYIFNRPAYNVSFHVNTNSGDL
                     IGTDTFVDNEVKLRKGTIYQCAEYGGFGILDEINMAKNDAVSVLHATLDYRRSIDVPG
                     YDKIDLHPAARFIGTMNYGYAGTKELNEALVSRFLVIDMPAQTEESLEFIFRRMFPNI
                     KENARTQFIGVFLDLQLKATNSEISTKPLDLRGLLAAMKIVETGLSPRKAVTMGVVNK
                     TFDIFEKEIVEDIVKTRIPADWTRDDVYER"
     gene            2629..>4409
                     /locus_tag="ACZPD3_03915"
     CDS             2629..>4409
                     /locus_tag="ACZPD3_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase CobT-related protein"
                     /protein_id="HIR1415184.1"
                     /translation="MKDEKIVEIDDYRLELENRIRNLLWTISGDYTLNMKVDVSLYLR
                     SKAIALYDGIKQGALAKYYDRNLLGLYLVKKVFCQAGEGELTAVAQLCVEEAIGDKIC
                     QERPGVREMQKEAFEDILDQEFERMPAYGDILGRLKIAILRDRLQNGNHYVEERLREI
                     RDMVYKARTAADTIELIRIIDSLYNTIIDPNFEKDHGTLEQVMAVTLEELTEYDWEDF
                     LTEELYEEALESYIEQMTNKITDMEDTAVTEEMEKQRQTKHKITILTEEELEKAYTYV
                     ELNFGKTYLTPAEEKRMNYLMCRELHRDCSLYFTEGILKNPVKRNYQYEYAVRLKNKN
                     IWLYHDKHRIVKQNIASLTDLLRKTLVLKSETQEVLSDRGTIIPSRLWRVGRSSEANL
                     FKRELKSDASDFVVDVLIDASGSQMSRQGDVALQAYIISEALSNVNLPHRVMSFCTFW
                     DYTILHRFREYDDPQSANENIFNYVTSSNNRDGLAIKTAGYGLLQRSEEKKILIVLSD
                     GKPYDVIVNRPHAKNPEPYMGKYAINDTATEVRHLRNLGVSVLGVFAGEEKDLSTEKK
                     IFGKDFAYIRDISNFSRIVGRYLIKQL"
     assembly_gap    4410..4419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4554..>5699
                     /locus_tag="ACZPD3_03920"
     CDS             4554..>5699
                     /locus_tag="ACZPD3_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR1415185.1"
                     /translation="MNENKEFKPYIPADKITPEFTVTSIIMGVILAVIFGAANAYLGL
                     RVGMTVSASIPAAVISMGVIRKIMKKNSILESNMVQTIGSAGESLAAGAIFTMPALFL
                     WAEEGLCEMPGIVEITLIALCGGVLGVLFMVPLRNALIVKEHATLLYPEGTACADVLL
                     AGEEGGANAATVFSGMGIAAIFKFVVDGLKVIPADVAVAFKGFKGEIGMECYPALLGV
                     GYIVGPRIASFMFVGSLIGWMVLIPVICLFGADTVMYPGTETISALYAAGGASKIWST
                     YVKYIGAGAIATGGIISLIKSLPLIIATFRDSMKSMKGGKNTSTERTAQDLPMNVILI
                     GIVAMVAIIWLVPAIPVNPIGALIIVIFGFFFATVSSRMVGLVGSSNN"
BASE COUNT         1750 a         1140 c         1358 g         1441 t
ORIGIN      
        1 gcaatcaaca cccaggcact ccgtctgctg agacttttcg gaaatacaac ttctaagaaa
       61 gtaactccgt cagtaggagc agagcaggaa tatttcctga tcgataaaga gaaatggctt
      121 cagagaaaag acctgatcta tacaggcaga actctttttg gagctatgcc tccgaaggga
      181 caggaaatgg atgaccatta ctttggaagt atccgtcaga gaatttccgc atacatgaaa
      241 gaggtaaatg aagaatgctg gaagcttgga attcctgcaa agacacagca taatgaggtt
      301 gctcctgcac agcatgagct tgcgccgatt tatgcaccgg tcaatatcgc agcagaccag
      361 aaccagatga tgatgcgtat ccttaaaaag gttgccagcc gtcatggaat gcgctgtgtc
      421 cttcatgaga aaccatttgc aggtgtgaac ggttccggta agcataacaa ctggtctctt
      481 accacagatg acggaatcaa tcttctggat ccgggcaaaa cacctcatga aaatattcag
      541 ttccttctgg tcctcacctg tatcctgaaa gcagtagatg aacatgcaga tcttctgcgt
      601 gaatctgcgg cagatgtggg aaatgaccag agacttggtg gccatgaagc accgccggca
      661 gtgatttccg tattccttgg agagcagctt gaggatgttc tggaacagct ggtcagcaca
      721 ggaacagcaa ctcacagtaa aaagggaagt aagctggaaa caggtgttaa gacgctgcct
      781 gatttcatga aggatgccac agaccgtaac cgtacgtcac cttttgcttt cacaggaaat
      841 aaatttgaat tccgtatggt gggctcaaga gattctattt ccgcatgtaa cgtagtcctg
      901 aatacgatca cagcagaagt atttaaggaa gtatgtgacc gcctggaaaa agctcctgat
      961 ttcgaactgg cggtacatga cctgatcaaa gaatatgcca cagatcatca gaagattgta
     1021 tttaatggaa acggctatgc gccggaatgg gagaaagaag ctaaacgccg cggacttccg
     1081 attcttccga gcatggtaga tgcaatccct gcacttacta cagagaaggc agtgaaactc
     1141 tttgaatctt ttgatgtatt ctcaagagcc gagcttgaat cacgtgcaga gattaaatat
     1201 gagatttatt ctaaagcaat caatattgaa gcaaagacca tgatcgacat ggcaaccaag
     1261 cagattattc cggcagttat cagatatacc actgttcttg gcgaatccat caatacagtg
     1321 aagacagcag gcgaagggct gatcgatgta agtgtacagc ttgacctgct taagaaagcg
     1381 tcaaaactct taaaggctgc aaacagtgcc atgagtaagc tgagcaaact ggttgagaag
     1441 acccaggaat atgcagaggg acgggacaga gccgtattct gcagagaaaa ggtggttcct
     1501 gcaatggaag ctttacgtga gccggtggat gaactggaaa tgatcgtaga taaggaaatg
     1561 tggccgatgc cttcttatgg agatcttgta tttgaagtat aatgttgtaa aaatataaag
     1621 aagaatgcta taatgaaagg gtcgtgaatc tgtaaaagat aagcggcctt tttctataac
     1681 acaaactgga ctataatagt tatataaagg agaactaaat ggaagaactg aaatttctgg
     1741 aggagcagca ggtagcccct gaactgataa agagagtaga agaattccgc agccggtatg
     1801 aggtggtacc ggagaccgca gacaggattg tgaaaccatc cattccattt tacggaaaag
     1861 acattctgga aatggccatt gccggactgc tcaaagggga gaatctatta ctcaccggtc
     1921 cgaaggcaac aggaaaaaat atccttgcag agaacctggc atatattttc aaccgtcccg
     1981 cgtacaatgt ttcttttcat gtaaatacta acagcggaga tctgattggc acagacactt
     2041 tcgtggacaa cgaagttaag ctccgcaaag gaactatcta tcagtgtgca gaatacggtg
     2101 gtttcggaat cctcgacgag attaatatgg caaagaacga tgcagtttcc gttcttcatg
     2161 caactttgga ttatcgccgt tccatcgatg ttccgggcta tgacaaaatt gatcttcatc
     2221 cggctgcccg ttttatcgga accatgaatt acggttatgc aggaaccaaa gaactcaatg
     2281 aagcgctggt atccagattt ctggtcatag acatgccggc tcagactgaa gaatccctgg
     2341 aatttatttt ccgccggatg ttcccgaata taaaagaaaa cgccagaaca cagttcatcg
     2401 gagtattcct ggatcttcag ctcaaagcca caaacagtga aatttccacc aaacctctgg
     2461 atctgcgagg tctccttgca gccatgaaga ttgtagaaac cggactttct ccgagaaaag
     2521 cagttactat gggagtggtg aacaagacct ttgacatttt cgagaaagaa atcgtagagg
     2581 atattgtgaa aaccagaatt ccggcagact ggacaaggga tgatgtatat gaaagatgag
     2641 aaaatcgtag aaatcgacga ttatcgtctg gaactggaaa acaggatccg caatctcctc
     2701 tggactatca gcggggatta cacgttaaat atgaaagtag acgtttccct gtatctgcgc
     2761 tcaaaagcaa tcgctctgta tgacggcatc aagcagggcg ctctggcaaa atattatgac
     2821 agaaatctgc tgggtttata tctggtaaaa aaagttttct gccaggcagg agagggagaa
     2881 ctgactgcag tagcacagct atgtgtagaa gaagcaatcg gggacaaaat ctgtcaggaa
     2941 cgtccgggag tccgggagat gcagaaggaa gcattcgagg atatcctgga tcaggagttt
     3001 gaacggatgc ccgcatacgg agatattctt ggccgattga agattgccat cctgcgtgac
     3061 cgtttacaga atggaaacca ttatgtagaa gagcgtcttc gggaaatccg cgacatggtt
     3121 tacaaagcca gaacagctgc agacactata gaattgatcc gtattattga cagtctttac
     3181 aacactatca ttgatccgaa ttttgagaaa gatcatggaa cactggaaca ggttatggca
     3241 gtcaccctgg aagaactgac agaatacgac tgggaagatt ttcttacaga agaactttat
     3301 gaggaagctc tggagagtta catcgaacag atgacaaata agattacgga catggaagac
     3361 actgctgtca ctgaagaaat ggaaaaacag cgccagacca aacacaaaat tacaattctc
     3421 acagaggaag aacttgagaa agcatacacc tatgttgagt tgaatttcgg aaaaacttat
     3481 ctgactcctg cagaagagaa gcgaatgaat tatctgatgt gtagggaact tcacagggac
     3541 tgcagccttt attttacaga aggaattctt aagaatccgg tcaaacgaaa ctatcagtac
     3601 gaatatgcag tgcgattaaa gaataagaat atctggctct accatgacaa acacagaatc
     3661 gtaaagcaga acatcgcatc cctcacagac cttttacgaa agacactggt gctgaaaagc
     3721 gagacgcagg aagtactgtc agacagagga acgatcattc cgtccagact ctggcgtgtg
     3781 ggcagaagca gtgaagcaaa tctgtttaaa agagaattga aatctgatgc atctgatttc
     3841 gtagtagatg tgctgatcga tgccagcggt tcccagatgt ccaggcaggg agatgtggca
     3901 ttacaggctt atatcatcag tgaagcatta agtaatgtaa acctgcctca ccgtgtcatg
     3961 agcttctgca cattctggga ctatacgatc cttcacagat tccgtgagta cgatgatcca
     4021 cagtcagcta atgaaaatat ttttaattat gtaacttctt ccaataacag ggatggactt
     4081 gccatcaaaa ccgcaggata cggtctgctt cagagatctg aggagaaaaa aatcctcatt
     4141 gtattaagtg atggaaaacc atatgatgtc atcgtaaaca gacctcacgc aaagaacccg
     4201 gaaccttata tgggaaaata tgcgatcaat gatactgcta cagaagtgcg gcacctgcga
     4261 aaccttggag taagtgttct gggagtcttt gcaggggaag aaaaggacct atccacagaa
     4321 aaaaagatat ttggaaaaga tttcgcatat atccgggata tttcgaattt ttccagaatt
     4381 gttgggagat acctgattaa acagttggan nnnnnnnnnt gttaagagat tatcaagtta
     4441 gcacgttaat atggtaaagt gcataaaaaa ccagtttttt caccttgcct ttttgtataa
     4501 atttcgctat aatgttctat gtttataaat caatacaaaa gggagaggaa atcatgaacg
     4561 aaaacaaaga gttcaaaccg tatattccgg ctgacaagat cacacctgaa tttacggtga
     4621 catccatcat catgggtgtt atccttgcag ttatcttcgg tgcagccaat gcttatcttg
     4681 gtctgcgtgt aggtatgact gtatctgctt ccattccggc agcagttatt tccatgggcg
     4741 taattcgcaa gatcatgaaa aagaactcca ttctggagag caacatggtc cagacaatcg
     4801 gttcagccgg tgaatcactg gcagccggtg caattttcac aatgcctgct ctgttccttt
     4861 gggcagaaga aggactttgt gaaatgccgg gtatcgtgga aatcacatta atcgcacttt
     4921 gcggtggtgt tcttggtgta ctttttatgg taccacttcg taacgcactg atcgtaaaag
     4981 agcatgcaac acttctttat cctgaaggaa ctgcctgcgc agatgttctt cttgcaggtg
     5041 aagagggtgg tgcaaacgct gcgacagtat tctcaggtat gggaatcgca gcgatcttta
     5101 aatttgtcgt tgacggttta aaggtaatcc ctgcagacgt tgcagtagca tttaaaggat
     5161 tcaaaggtga aattggtatg gaatgctatc ctgcacttct cggtgttgga tatatcgtag
     5221 gaccaagaat cgcatccttt atgtttgttg gttctcttat tggctggatg gtgctgattc
     5281 cggtaatctg tctgttcgga gctgatacag ttatgtatcc cggaacagaa acaatttctg
     5341 ctctttatgc agcaggcgga gcatctaaaa tctggagtac ttatgtaaaa tatatcggag
     5401 caggagctat cgcaacaggt ggtattattt ctctgatcaa atccctgcct ctgatcattg
     5461 caaccttccg tgattccatg aagagcatga agggtggcaa gaatacaagt acagaaagaa
     5521 ctgcacagga tcttccaatg aacgttatcc tgatcggtat tgtggcaatg gttgcgatca
     5581 tttggcttgt tccggctatc ccggttaacc cgatcggtgc gctgatcatc gtaatcttcg
     5641 gcttcttctt tgcaactgta tcttcccgta tggttggtct tgtaggaagc tccaacaac
//
LOCUS       DBMDXG010000091         5690 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3544_length_5690_cov_1.794597, whole genome shotgun sequence.
ACCESSION   DBMDXG010000091 DBMDXG010000000
VERSION     DBMDXG010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3544_length_5690_cov_1.794597"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="ACZPD3_03925"
     CDS             <1..629
                     /locus_tag="ACZPD3_03925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1415186.1"
                     /translation="SGFAQVNHIRGEVSSACEVLYGLFDPEKVVRTIAVPIYTGMVHD
                     TGVFQYSSTSPETMRIAGELMKTGFNFSKIIDESFYQKTYVQNQVMGRVLAESILLLD
                     GKCIIGYLKKRDMEFYGVDGKDLDGIVSQLRLTAGVEVAMFIYEVETQSFKVSLRSNG
                     NVDVSKIAVYFGGGGHMRAAGCDLQGSVYDVINNVTEQICKQFQEQEV"
     gene            626..1513
                     /gene="truB"
                     /locus_tag="ACZPD3_03930"
     CDS             626..1513
                     /gene="truB"
                     /locus_tag="ACZPD3_03930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR1415187.1"
                     /translation="MMDGVIVIRKEKGFTSHDVVAKLRGILHMKKIGHTGTLDPDAEG
                     VLPVALGKATRLVDMITDKEKTYEAVMRLGVVTDTQDMSGTVLSQTTELSVTEEELCT
                     VVSSFVGDYMQVPPMYSALKVNGKKLYELAREGKTVERKPRPVHFYEIEILDISFPLV
                     RFRVTCSKGTYIRTLCHDIGEKLGCGAAMESLLRTKVGRFTLDDAITLAQTEEAVQEG
                     TIESKILGIEEILAEYPRVCCTKEGDRLLANGNPLVQALVDAQEKNGWIRMCSSEGSF
                     AGVYQWDEKRNRYFPVKMF"
     gene            1522..2418
                     /locus_tag="ACZPD3_03935"
     CDS             1522..2418
                     /locus_tag="ACZPD3_03935"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR1415188.1"
                     /translation="MEYLRIENDFPRISHSAVTLGKFDGIHRGHQKLVEKIIEQKQEG
                     AQAVVLALGTASRTILTKEERCRILEEMGVDILLECPLTEKIRHMKAENFIKEILIGD
                     LQVSYVAVGEDFRFGYERKGTPAMLKEFGKKYGFHTEVLPKKMDGRRKISSTFVREEL
                     NRGNMEKFRFLMGTDFSVEGIVEHGRGMGHKYLLPTTNLIPPVEKLMPPNGVYITVSH
                     FRDRSYQGITNVGHKPTVGGEKFIGVETYLFDCNEDLYGEYCKVDFKKFLRPEQKFSS
                     LEALKAQLERDAVKGQEYFETL"
     gene            2560..2826
                     /gene="rpsO"
                     /locus_tag="ACZPD3_03940"
     CDS             2560..2826
                     /gene="rpsO"
                     /locus_tag="ACZPD3_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR1415189.1"
                     /translation="MISKEKKAAIMKEYARTEGDTGSPEVQVAVLTARIQELTEHLQS
                     NHKDHHSRRGLLKMVGQRRGLLAYLKKTDIERYRALIEKLGLRK"
     gene            3239..5332
                     /locus_tag="ACZPD3_03945"
     CDS             3239..5332
                     /locus_tag="ACZPD3_03945"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR1415190.1"
                     /translation="MYKSYSMELAGRTLTVDINRVAKQANGAALMHYGDTTVLSTATA
                     SKEPREGIDFFPLSVEYEEKMYAVGKIPGGFNKREGKASEHAILTSRVIDRPMRPLFP
                     KDYRNDVTLVNMVMSVDPECNPEIPAMLGSSIATCISDIPFDGPCATTQVGLINGEYI
                     INPTMAQKDVSDLQLTVASTREKVIMIEAGAKEVPEDKMIEAIYKAHEVNQEIIKFID
                     KIVEECGKPKHSYESCAVPEELFAAIKEVVPPAEMEVAVFSDDKQTREENIRQVTEKL
                     KEAFADKEEWLAVLGEAVYQYQKKTVRKMILKDHKRPDGRAITQIRPLAAETDIIPRV
                     HGSAMFTRGQTQICTITTLAPLAEAQKLDGLDEFETSKRYMHHYNFPSYSVGETKPSR
                     GPGRREIGHGALAERALVPVLPSEEEFPYAIRTVSETFESNGSTSQASICASTMSLMA
                     AGVPIKKPVAGISCGLVTGDTDDDYIVLTDIQGLEDFFGDMDFKVAGTHDGITAIQMD
                     IKIHGLTRPIVEEAIRRTKEAREYILTEVMEKCIAAPRTTVGEYAPKIIQIQIDPQKI
                     GDVVGQRGKTINTIIERTGVKIDITDEGAVSICGVDQKSMDEAANMVKIIATDFEAGQ
                     IFTGKVVSIKEFGAFVEFAPGKEGMVHISKICKERINRVEDVLTLGDKVKVICLGKDK
                     MGRISFSMKDVPEEA"
     gene            5339..>5690
                     /gene="nrdG"
                     /locus_tag="ACZPD3_03950"
     CDS             5339..>5690
                     /gene="nrdG"
                     /locus_tag="ACZPD3_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR1415191.1"
                     /translation="MRYHNITKDDMLNGDGLRVVLWVAGCNHCCPECQNPVTWDPNGG
                     LPFGEAERKEIFAELDKDYVSGITFSGGDPLHPANITEVTAFAKEIRKRYPGKTIWLY
                     TGFLWEEISKEEIVR"
BASE COUNT         1782 a         1054 c         1451 g         1403 t
ORIGIN      
        1 actccggatt tgcacaggtg aatcatatca ggggagaggt aagctctgcc tgtgaggttc
       61 tctatgggct ttttgatcct gagaaagtgg tccgtaccat tgcagtgcca atctatacgg
      121 gaatggttca tgacacaggg gtatttcagt actcttcaac ttcaccggag accatgcgta
      181 ttgccgggga actgatgaag acaggattca atttcagtaa gatcattgac gaatctttct
      241 atcagaaaac ttatgtgcag aatcaggtaa tggggcgtgt tcttgcagag agtatcctgc
      301 ttctggatgg aaaatgtatt atcggatatc ttaaaaaaag agacatggag ttctatggtg
      361 tggatggcaa ggatttggat ggtatcgtca gtcagctccg tcttacagca ggagtggaag
      421 ttgccatgtt tatctatgaa gtggaaactc agtctttcaa agtttccctg cgttccaacg
      481 gaaatgtgga tgtaagcaag attgcagtat attttggcgg cggcggtcat atgagagcag
      541 ccggatgtga tcttcagggt tctgtgtatg atgtgatcaa caatgtgaca gaacagatct
      601 gcaaacagtt tcaggagcag gaagtctgat ggatggagta attgtaatcc gtaaggaaaa
      661 aggattcact tcccatgatg tggtggcaaa gcttcgggga atccttcata tgaagaaaat
      721 cggacatacc ggaactcttg atccggatgc agaaggagtg ctcccggtgg ctctgggaaa
      781 agcaacccgt ctggtggata tgatcacaga taaagagaaa acttatgagg cagtgatgcg
      841 tctgggtgtt gttacggata cccaggatat gagcggaact gttctctcac agactacaga
      901 gctttccgtt acagaagaag agctttgcac agtggtcagt tcttttgtgg gagactatat
      961 gcaggtgccg ccaatgtatt cagcactgaa ggtcaatgga aagaaactct atgaacttgc
     1021 cagagagggc aagactgtgg agcgtaaacc aagacctgta catttttatg agatagaaat
     1081 tctggatatc agttttccgc tggtgcgttt tcgtgtgacc tgcagcaagg gaacctatat
     1141 ccgtactctg tgccatgata taggggaaaa actgggctgt ggagctgcca tggaaagtct
     1201 tttgcggaca aaggtgggca gatttacact ggatgatgcc attaccctgg cgcagacaga
     1261 agaggcagtg caggaaggaa ctatagagag taaaatcctc ggtattgagg agattcttgc
     1321 agagtatcca agggtatgct gtacaaaaga aggggacaga cttctggcta atggaaatcc
     1381 tcttgtacag gcactggtcg atgcacagga gaaaaacgga tggatccgta tgtgcagcag
     1441 tgaaggcagt tttgccggag tttaccagtg ggacgagaaa agaaacaggt atttccctgt
     1501 aaaaatgttt taggagacat gatggaatat ttaaggattg agaatgattt tcccagaatt
     1561 tcacacagcg cggtaacact tgggaagttt gacggcatac accgcggaca tcagaaactg
     1621 gttgagaaaa ttattgagca gaaacaggaa ggtgcacagg cggttgtact tgcgcttgga
     1681 actgcctcaa gaacaatcct cacaaaggaa gagagatgtc gtatattgga agaaatggga
     1741 gtggatatac tgttggaatg tccgctcaca gagaaaatac ggcacatgaa agcagagaat
     1801 tttataaaag aaatactgat aggagatctg caggtttcct atgtggcagt tggagaggat
     1861 ttccgttttg gctatgagcg gaaaggaact cctgccatgc tcaaagagtt cggaaagaaa
     1921 tatggatttc atacggaagt cctgccaaaa aaaatggacg gacgccgtaa gattagtagt
     1981 acctttgtca gggaagaact gaacagagga aatatggaaa aattccgttt ccttatggga
     2041 acagatttct cagtagaagg aatcgttgaa catggacgtg gaatgggaca taaatatctg
     2101 cttccgacca ccaacctgat ccctccggta gagaaactga tgcctccaaa cggggtttat
     2161 atcacagtat cccatttcag agacagaagt tatcagggga ttaccaatgt aggtcataag
     2221 cctactgttg gcggcgagaa gtttattggt gtggaaacat atctgtttga ctgcaatgaa
     2281 gatctgtatg gggaatactg caaggtagat ttcaaaaaat tcctcagacc tgaacagaag
     2341 ttttcttcgc tggaggcact gaaggcacag ctggagaggg atgctgtgaa gggacaggaa
     2401 tattttgaaa ctttgtaaaa aagaggttta cacgccagaa tcaatgtgct atactaacca
     2461 aggtgaattt agcccatgtt cagggaccgg acgctccgac catctcttaa tgtgtggcgg
     2521 tcaatcaata ataattatag aatacaggag gaaatcacaa tgatttcaaa agaaaagaaa
     2581 gcagcgatca tgaaagaata tgcaagaact gaaggcgata caggatcacc ggaagtacag
     2641 gtagcagttc ttacagcaag aatccaggaa cttacagagc atttacagag caaccataaa
     2701 gatcatcact ccagacgtgg tcttctgaaa atggtaggtc agagacgtgg actgttagct
     2761 tacctgaaga aaacagacat cgaaagatac cgtgcactga ttgaaaaatt aggcttaaga
     2821 aaataatagc cttttagtat gcggaagggg gcggatttcc atccgcctcc ctttttatgt
     2881 atatggcagt tctgtaaaat atggtgtttt tcaggattgc ggtggcttgt tgtttcgact
     2941 gcatctgcat attcagaagc tgcgaaacat gacaggagtt atttccgaga ctactgaaaa
     3001 gattattttg tatatgaaag taacaaaaca ggcagatata tttgcctgaa aagtagtttt
     3061 ttcagttgtt tcggactcct gttttaatgt gacaatttca taaaaagtct gaacgttgat
     3121 ggacaagatt gtcagcagac ggaacagaca aatttgaaaa gaataaagta gttaccggac
     3181 atgcagtgaa cggtaacaga aaatgacaac agaattatga aaaaaggaga atgaacatat
     3241 gtacaaaagt tattccatgg aattagccgg aagaaccttg acagtagata tcaaccgcgt
     3301 tgcaaaacag gcaaacggtg cagcattaat gcactatggt gatactacag ttttatctac
     3361 agcaacagca tccaaagagc caagagaagg aattgatttc ttcccgttaa gcgttgaata
     3421 tgaagagaaa atgtatgcag taggcaaaat cccaggagga tttaacaaga gagagggaaa
     3481 agcaagcgag catgcaatcc ttacttcccg cgtgatcgac agaccaatgc gtcctctgtt
     3541 tccgaaggac tacagaaacg acgtaacact ggtaaacatg gttatgtctg ttgatcctga
     3601 gtgtaatccg gagattccgg ctatgcttgg ttcctctatt gcaacctgca tttccgatat
     3661 tccgtttgat ggtccgtgtg ctaccacaca ggttggtctg atcaatggtg aatatatcat
     3721 caaccctacc atggcacaga aagatgtatc tgaccttcag cttacagtag cttccacaag
     3781 agagaaagta atcatgatcg aagccggcgc aaaagaagtt ccggaagaca agatgatcga
     3841 agctatctat aaagctcatg aagtaaacca ggagatcatt aaatttatcg ataagatcgt
     3901 agaagaatgc ggaaaaccga aacacagcta cgaatcatgt gcagttccgg aagaactttt
     3961 cgcagcgatc aaagaggtcg tacctccggc agaaatggaa gttgccgtat tctctgacga
     4021 taaacagaca agagaagaaa atatccgtca ggtaacagag aaactgaaag aagcttttgc
     4081 cgacaaggaa gagtggcttg cagttctggg tgaggctgta tatcagtatc agaagaagac
     4141 tgtccgcaag atgatcttaa aagaccacaa acgtcctgat ggtcgtgcca ttactcagat
     4201 tcgtcctctg gcagcagaga cagatattat cccgagagta catggttctg ctatgttcac
     4261 acgtggacag acacagatct gtaccatcac aactctggct cctcttgcag aagcacagaa
     4321 gcttgacgga cttgacgaat ttgaaacttc aaagagatat atgcatcact acaatttccc
     4381 gtcctattct gtaggtgaga caaaaccatc cagaggaccg ggacgtcgtg agatcggaca
     4441 tggtgcactt gctgagagag cattagttcc tgtacttcca agcgaagagg aattcccata
     4501 tgcgatccgt actgtatctg agacatttga atccaatggt tctacttctc aggcaagtat
     4561 ctgtgcatcc acaatgtctc tgatggcagc cggtgtaccg atcaaaaaac cggtagcagg
     4621 tatttcctgt ggtctggtaa caggcgatac agatgatgat tacatcgttc tgacagatat
     4681 ccagggactt gaagacttct tcggagatat ggacttcaag gtagccggaa ctcatgacgg
     4741 tatcacagca atccagatgg atatcaagat ccatggactg acaagaccga tcgtagaaga
     4801 agcaatcaga cgtacaaaag aagctagaga atatatcctg acagaagtta tggagaaatg
     4861 catcgcagct ccccgtacaa ctgtaggcga atatgctccg aagatcatcc agatccagat
     4921 cgatcctcag aagatcggtg atgttgtagg tcagagagga aagactatca atacgatcat
     4981 cgagcgtaca ggtgttaaga tcgatatcac agacgaaggt gcggtatcta tttgtggagt
     5041 tgatcagaag agcatggatg aggcagctaa catggttaag atcattgcaa ctgattttga
     5101 agcaggacag atctttacag gtaaagttgt cagcatcaaa gagtttggtg cattcgttga
     5161 atttgcaccg ggtaaagagg gaatggtaca catttccaag atctgtaaag aaagaatcaa
     5221 tcgtgttgag gatgttctca cacttggtga taaagttaag gttatctgcc ttggcaaaga
     5281 caagatggga agaatcagtt tcagcatgaa ggatgtaccg gaagaagcgt aaaaaattat
     5341 gagatatcac aatattacca aggacgatat gcttaatggc gacggacttc gtgtagtgct
     5401 ctgggtggca ggatgcaatc actgctgtcc ggagtgccag aatcctgtca cctgggatcc
     5461 aaatggcgga ctgcctttcg gtgaggcaga acgcaaggag atttttgcgg aactggataa
     5521 ggattatgtg agcggaatta ccttttcagg tggagatcca ctgcacccgg caaatatcac
     5581 tgaggtaaca gcctttgcga aggagatcag aaagagatat cctggtaaaa caatctggct
     5641 ttatacaggt ttcctctggg aggagatcag caaagaagag attgtgcgtt
//
LOCUS       DBMDXG010000092         5638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3576_length_5638_cov_2.061766, whole genome shotgun sequence.
ACCESSION   DBMDXG010000092 DBMDXG010000000
VERSION     DBMDXG010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3576_length_5638_cov_2.061766"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            218..2029
                     /locus_tag="ACZPD3_03955"
     CDS             218..2029
                     /locus_tag="ACZPD3_03955"
                     /inference="COORDINATES: protein motif:HMM:NF048350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7507 domain-containing protein"
                     /protein_id="HIR1415192.1"
                     /translation="MKKRIWNQLKKAVMITAAVGILGAGAVSAAEFEAAELYVPAVGL
                     KQSAQWIDEEKFQAELTLEVSGLKELYKSQQENTASENGQLQMENSTWDGEVREAEAA
                     ENTTDEVDSENTECELESAVIDIEDIESEEYRKAEENAEGSDTETGQTQQSEQENQNE
                     IREDKTRYFLTVYISEYFQVNAEALKNDLQAESVQIQNKKGETTQITKLTCETALSDA
                     ETDIFTMKVPVSLREEYRISSVKTDYPLCQDAPLCKDWEGTGAYFWMKSGDETRTVAE
                     TPSALLSVQEAKTGITARLQQETKEVRAGQNLTYILSVENTGELPLENIEISSVFSQD
                     NIKAEWKQEEGFTANGMQGIISGLKAGEIRNLQMTVQLTEEQAGELIHTVTVKAKYPG
                     KEESIGCQKSVETEVIALKAAFEVEKTADRTQAYPGDTITYQICIRNTGERTLHSVLS
                     TERFQNAGIQANFVRKEGVTLNSTGTQALIPQIAPGEAFALYATVTVPQYMASQELIN
                     EVIVTSDETGTQAMTSKANVELKETDNIVTVTPQPASLASQSYGYDSKSGSAYTTASK
                     PRTGDETETALYIVLGIFAIMSGVSAFCYRKTKKQQK"
     gene            2326..3783
                     /locus_tag="ACZPD3_03960"
     CDS             2326..3783
                     /locus_tag="ACZPD3_03960"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR1415193.1"
                     /translation="MKLISLLEKLEYTCLQGSTDQEVKNVIYDSRKVEEGSLFICIRG
                     AVVDGHKFVPDVVAKGAKVLIVEEAVEAPEDVTVILVKDTRYAMAFISAAYFGYPAEK
                     LKTIGITGTKGKTTTTYMVKSILENAGYKVGLIGTIEAIIGDKVIPAKNTTPESYVIQ
                     EYFHEMAEAGCDCVVMEVSSQGLMLHRTQGFVFDFGIFTNIEPDHIGPNEHKDFDDYL
                     RCKSLLLKQCKVGIVNRDDEHFEKIIEGHTCSLETYGFSPEADLRAEDAKLVGGKGYL
                     GISYHLKGLLDFHVEIDIPGKFSIYNSLTAIAICRHFKVSEENILKALKVAKVKGRIE
                     MVKVSDDFTLMIDYAHNAMALESLLTTLKEYHPHRLVCLFGCGGNRSKLRRYEMGEVS
                     GKLADLTIITSDNPRDEEPQAIIDDIKIGMAKTDGKYVEIPDRKEAIAYAIHHGEPGD
                     IIVLAGKGHEDYQEIKGKKYPMDERVLIADILAGK"
     gene            3810..>5638
                     /gene="priA"
                     /locus_tag="ACZPD3_03965"
     CDS             3810..>5638
                     /gene="priA"
                     /locus_tag="ACZPD3_03965"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR1415194.1"
                     /translation="MIYADIIIDISSDKLDRSFQYRVPERLEKKIKAGMVAAIPFGNA
                     GRVRKGYVIGLSDKPKIAPEKIKEISEICSGEETTESRLIALAAWMRENYGSTMIQAL
                     RTVLPIQEKIKAKEKKYICLDISEEAGAQLLKELEQTRFKARTRLLRELLEKKRLDYT
                     HAAKELGTTSAVVKKFQEQGIIRIEYDEIMRTSLNTGDISGERRMPLTDEQEAAVKQI
                     QREWKKPSPKPVLIEGVTGSGKTQVYIKLIEQTLSQGKEAIVLIPEIALTYQTVRRFY
                     ARFGDKVSVINSRQSQGERYDQFKRAKKGEVQVMIGPRSALFTPFASLGLIIIDEEHE
                     SSYKSESTPRYHARETAIRRAVLEHANVVMGSATPSVEAYSKAMNGEYSLVRLTTRYG
                     SRPLPRVSIVDLREELKAGNRSVLSRELRQKMKGRFEKKEQVMLFLNRRGYAGFVSCR
                     SCGHVMKCPHCDVSLSEHNNERLLCHYCGYETGKPRICPVCGSPYIGGFKAGTQQIEK
                     VVREDFPEVRTLRMDFDTTRTKGSYEKILAAFAAHEADILIGTQMIVKGHDFPDVTLM
                     GIVAADLSLNAEDYRCAERTFQLLCQAVGRGGRGEKPGEAVIQT"
BASE COUNT         1858 a          994 c         1460 g         1326 t
ORIGIN      
        1 tatccggtga ggagttattt gtttataggt aacaaaaaat gccgtattta tgcggcttgt
       61 ggcgtttcgc aaacgcctat tattgtgcaa aacagagagg agagatgcgt atgtctgtgg
      121 atatgaactt ataatctcga aagatactgt accgatatca ttctgacagt ggaagtacct
      181 cagatgacat agaataaaaa ttcaggagaa tgagagaatg aaaaaaagaa tatggaatca
      241 gttaaaaaaa gcagtcatga taacggctgc tgtcgggatt ttgggagcag gagcagtatc
      301 agcagcagaa tttgaagctg cagagttata tgttcctgct gtgggtttaa agcagagtgc
      361 acagtggatc gatgaagaga aatttcaggc agaactgaca ctggaagtga gcggtctgaa
      421 agaactgtac aaaagtcaac aggaaaatac tgcttcggag aacggacagt tgcagatgga
      481 aaatagtaca tgggatggag aagtgagaga agcagaagca gcagaaaata cgacagatga
      541 agtggattct gaaaatacag agtgtgaatt agagagtgca gtaatagata tagaagatat
      601 agaaagcgaa gaataccgga aggcagaaga aaacgcagaa ggatctgaca cagaaaccgg
      661 acagacacaa cagagtgagc aggagaacca aaatgagatc agggaagata aaaccagata
      721 ttttctgact gtatatattt cagaatattt tcaggtaaat gcagaagcgt taaagaatga
      781 tctgcaggct gaatccgtac agatacaaaa taagaaaggt gagacaaccc agattacaaa
      841 gctgacctgt gagactgcac tttcagatgc ggaaacagat atctttacca tgaaagtgcc
      901 ggtgtctctg cgtgaagaat atcgtatttc ttctgtaaaa acagattatc ctctgtgcca
      961 ggatgcaccg ctttgcaaag attgggaggg gacaggtgcg tatttctgga tgaaatcagg
     1021 ggatgaaacc cggactgttg cagaaactcc ttcagctctg ctttccgtac aggaggcaaa
     1081 aacagggatc acagcccggt tacagcagga gacgaaagag gtacgtgcag ggcagaacct
     1141 tacttatata ttgtctgtag agaatacagg agaattacct ctggaaaata tagagatatc
     1201 cagtgttttt tctcaggata atataaaagc cgaatggaaa caggaagagg gatttactgc
     1261 aaatggtatg cagggaatta tttcaggtct taaggcagga gaaatcagaa atcttcagat
     1321 gacagtacag cttacagaag agcaggcagg agagcttatt catacagtaa ctgtaaaagc
     1381 gaaatatccg ggaaaggaag aaagtattgg atgtcagaaa tcagtcgaaa cagaagtcat
     1441 tgcattaaag gcggcttttg aggtggagaa aactgcggac agaacacagg catatccggg
     1501 agatacgatc acatatcaga tctgcatcag gaatacgggt gagcggacac ttcattcggt
     1561 tctcagtaca gaaagattcc aaaatgccgg tattcaggca aactttgtca ggaaagaggg
     1621 cgtaacatta aacagcacag gcacacaggc tctgattccg cagatcgcac cgggagaggc
     1681 ttttgcactt tacgccacag tgactgttcc acaatatatg gcaagtcagg aattgatcaa
     1741 tgaggtaatc gttacatctg atgagactgg tacacaggca atgacatcta aggcaaacgt
     1801 agaattaaag gaaactgata atattgttac agttacgccg caaccggcat ctctggcttc
     1861 ccagagttat ggatatgact caaagtccgg aagtgcttat actactgctt ccaaaccacg
     1921 tacaggagat gagacagaaa cggctttgta tattgtactg gggatttttg caatcatgtc
     1981 cggagtcagt gcattctgtt acaggaagac aaaaaaacag cagaagtaga gaaacaacgt
     2041 tgaaacgaca ggggaggtgt gctattctta tatagaatat acgctttagt aaaacacctc
     2101 tgcctgactt taaagaaatt gcgttactgt tcacacatca ctatcccgcg aggtgttttg
     2161 gcatgaatat gccaaaatcc cgagacatac aagccaaaat tctattgccg caggcaatct
     2221 ggaatgaatt ttggctaagt tactgtgaac ggagtgaaca gtaacgaaat ggccgtagag
     2281 gctgtataaa attcaggcag gcaggaaatg atatggagga ttaaaatgaa attaatatcc
     2341 ttactggaaa agctggaata tacatgtctt cagggcagta cagaccagga agtaaaaaat
     2401 gtgatttatg attcccggaa agtagaagag ggctctcttt ttatctgtat ccgcggtgca
     2461 gtggtggatg gccataaatt tgtgccggat gtagttgcaa aaggagcaaa ggttctgatc
     2521 gtggaagaag ctgtggaagc accggaagat gtgacagtca ttcttgtgaa agatacacga
     2581 tatgcaatgg cgtttatttc tgccgcatat ttcggatatc ccgcagagaa actgaaaaca
     2641 attggtatca caggaacaaa aggaaaaaca accactacgt atatggtaaa atctattctg
     2701 gaaaatgccg gatataaagt gggactgatc ggaaccattg aagcgatcat cggtgacaaa
     2761 gtgattcctg caaagaacac aacaccggag tcttatgtga ttcaggaata tttccatgag
     2821 atggcagaag ccggctgtga ctgtgtggtt atggaagttt cctcccaggg actgatgctt
     2881 cacagaactc agggatttgt tttcgatttt ggtattttta caaatattga gccggatcat
     2941 atcggaccaa atgaacataa ggattttgat gattatctca gatgcaagtc cttacttctg
     3001 aaacagtgca aggtaggaat tgtaaacaga gatgatgaac atttcgagaa aattattgaa
     3061 ggtcatacat gcagcctgga aacttatgga ttttcaccgg aagcagacct tcgtgcggaa
     3121 gatgcaaagc tggtcggagg taaaggatat ctgggtattt cctaccatct gaaaggactt
     3181 ctggattttc atgtggagat agatattccc ggtaaattca gcatctacaa ttcattgaca
     3241 gcaatcgcga tctgccgtca ttttaaggtt tcggaagaaa acattctcaa agccttaaaa
     3301 gtagctaagg taaaaggccg tatcgaaatg gtaaaagtat ccgatgattt tactctgatg
     3361 atcgattacg cgcataatgc catggctctg gagagtcttc tgaccacttt gaaagaatat
     3421 catccccaca gactggtatg tctgtttgga tgcggaggca acagatcaaa gcttcgccgc
     3481 tatgagatgg gagaggtttc cggaaagctt gcagacctta ccatcattac ctctgataat
     3541 ccacgtgatg aagaaccaca ggcgatcatc gatgatatca agatcggtat ggcgaaaacc
     3601 gatggaaaat atgtagagat tccggacaga aaagaagcca tcgcctatgc gatccatcat
     3661 ggagagccgg gggatattat agttctggca ggtaagggac atgaggatta tcaggaaatt
     3721 aaaggaaaga aatatcctat ggatgaacgt gtcctgatcg cagatattct tgctggtaaa
     3781 taggtagcgg gtcaaacagg aataagttca tgatatatgc tgatataata attgatattt
     3841 ccagtgataa actggaccgg agttttcaat atcgtgtacc ggaaaggctg gagaagaaaa
     3901 taaaggcagg gatggtggca gccatccctt ttggaaatgc cggccgggtg cgtaaaggat
     3961 atgtgatcgg attgtcagat aaaccgaaaa ttgctcccga aaagattaaa gaaatcagtg
     4021 aaatctgcag tggagaagag actacggaat ccagactgat cgccctggca gcctggatga
     4081 gagaaaatta tggttccacc atgatccagg ctctcaggac agtacttcct atccaggaga
     4141 aaatcaaggc aaaagagaaa aaatatatat gcctggatat ttccgaagag gcgggagcgc
     4201 agttgctgaa agaacttgaa caaacaaggt ttaaagccag aacaagactt ctgagggaac
     4261 ttctggagaa aaaaagactg gattatacac atgcggcgaa agaactgggg acaacttctg
     4321 ctgttgtaaa gaaatttcag gagcagggga ttatccgcat agaatatgat gagataatga
     4381 gaacttcact gaatacaggt gatatttccg gagaaaggcg aatgccgctt acagatgaac
     4441 aggaagcggc agtaaagcag atacagagag agtggaaaaa accatcgccg aagcctgtac
     4501 tgatcgaggg agtgacaggg agcgggaaaa cccaggtgta tataaagctg atcgagcaga
     4561 cgctttctca gggaaaagaa gcaatcgtgc tgatcccgga gattgctctg acatatcaga
     4621 cggtacgcag attttatgcc agatttgggg ataaagtatc agttatcaat tccagacagt
     4681 ctcagggaga acgctatgat cagtttaaac gtgcgaaaaa gggagaagtc caggtgatga
     4741 tcggtcccag atctgcgctg tttacgccat ttgccagtct gggactgatc attattgatg
     4801 aagaacatga atcaagctat aaaagtgaaa gcacaccccg ttatcatgca agggaaactg
     4861 caatcaggag agctgtttta gaacatgcaa atgtggtaat gggttcagct actccatcag
     4921 tggaagccta cagtaaggca atgaatgggg aatatagtct ggtcaggctg acaacaagat
     4981 atggaagcag acctctgccc cgggtttcta tcgtagacct gcgtgaggaa ttaaaagcag
     5041 gaaacaggtc tgtactgagc agagaactcc ggcaaaagat gaaaggccgg tttgagaaaa
     5101 aagaacaggt aatgcttttt ctgaacagaa gaggatacgc aggttttgta tcctgccggt
     5161 cctgtggaca tgtgatgaag tgtccgcatt gtgatgtttc tctttcggag cataataatg
     5221 aaaggctttt atgtcattac tgtggatatg agacaggaaa accccggata tgcccggtgt
     5281 gcggatcgcc ttatattggc ggatttaagg caggaacaca gcagatcgag aaggtagtgc
     5341 gggaagattt tccagaagtc aggactctca gaatggattt tgatacaaca agaaccaagg
     5401 gcagctatga aaagatcctt gcagcttttg ctgctcatga ggcagatata ctgatcggaa
     5461 cgcagatgat cgttaagggg catgattttc cggatgtcac actcatggga atagtagcag
     5521 cggatctttc gctgaatgca gaggattatc gatgtgcgga acgtaccttt cagcttctct
     5581 gtcaggcagt cggccgggga ggacgggggg aaaaaccggg agaggcagtg atccagac
//
LOCUS       DBMDXG010000093         5607 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3601_length_5607_cov_1.686342, whole genome shotgun sequence.
ACCESSION   DBMDXG010000093 DBMDXG010000000
VERSION     DBMDXG010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3601_length_5607_cov_1.686342"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="ACZPD3_03970"
     CDS             <1..144
                     /locus_tag="ACZPD3_03970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HIR1415195.1"
                     /translation="KADNERGTIFNQVKIRVDDSFTLEMHIDTDEANAAKIATGTTVT
                     IIK"
     gene            277..543
                     /locus_tag="ACZPD3_03975"
     CDS             277..543
                     /locus_tag="ACZPD3_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR1415196.1"
                     /translation="MIVGKVVGSVVSTRKSEKLIGSKFMIIEPVHHMKGDLSQLVAID
                     MIGAGVGEYVLVAQGSAARIGCGVETAPVDAAIVGIIDDGAGLE"
     gene            548..1876
                     /locus_tag="ACZPD3_03980"
     CDS             548..1876
                     /locus_tag="ACZPD3_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1415197.1"
                     /translation="MEIKELQDIIQQNGVVGAGGAGFPTYMKLTDKANTILMNCAECE
                     PLLKLHRQLLEKHAYEIMKTFHMIQETVGASEAIIGIKKSYVQTIHALNQHIEEFPGM
                     RLHLLDEVYPMGDEVVLIYEATGRVVRPGGLPIEQGVAVFNVETVYNVYRAVEEKQPV
                     TDKYVSVVAEVSDPVTVRVPLGCTLEEVVAQAGSTTVKDPVYFVGGPMMGRIGNGSDP
                     VTKTTNAILVLPKDHLIVAKKQRTSSIDLKRAASICCQCNTCTDLCPRHNLGHPIDPA
                     KFMRAASNNDFRDLNPYIDASFCSSCGVCEMYSCPQSLAPRSLLADMKGGLRKAGIRP
                     PQGVQPKPVQESREYRKVPEERLMARLGLTRYDKDAPLKEELVQVKKVRILLSQHIGA
                     PAQAVVKAGDEVTRGQMIAQPAQGLSVGIHASVSGKVTEVTDRYIIIAVK"
     gene            1893..2441
                     /locus_tag="ACZPD3_03985"
     CDS             1893..2441
                     /locus_tag="ACZPD3_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1415198.1"
                     /translation="MSKAIGMIEFKTTSTGVTAADAMVKTSEVEIVEAQTVCPGKYIA
                     IITGDLSAVKAAVDTAVTTYEDKCIDSFVLGNPHESIFPAIYGTTQVEDISALGILET
                     YDAASIIEAADQAAKTAIVDLIELRIAKGMCGKSYMMITGEVSAVEASIDRAKELVAA
                     KGMYLDSSVIAHPDRRMIDSIL"
     gene            2509..3273
                     /locus_tag="ACZPD3_03990"
     CDS             2509..3273
                     /locus_tag="ACZPD3_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR1415199.1"
                     /translation="MLALERRNLILEKLQAEKRVVVSELSQLYDVSEETIRRDLDKLE
                     KEGLAIKSYGGAVINEDVSIDLPFNVRKNQNVTGKQKMAELAASLVKDGDHIFLDAST
                     TAVFVAKALKEKERLTVITNSMEILLELADVSGWNIISTGGVMKEGYLAFLGSKTDES
                     IRSYYVDKVIFSCKALDLEWGIMESQEAFGSTKRAMIGSGRKRILVVDSSKFDQTAFS
                     VAGSMKEVDIIVTDKEPTERWKKHFEKFNVKCLYPV"
     assembly_gap    3464..3473
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3474..4435
                     /locus_tag="ACZPD3_03995"
     CDS             <3474..4435
                     /locus_tag="ACZPD3_03995"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - 1,2-propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HIR1415200.1"
                     /translation="DAPVDKIAEGVKKFLEYQPEAVVAVGGGSAIDSSKAIREFALKI
                     NNYGKVGLIAIPTTSGTGSEVTSFAVVNDTAAHVKYPLISESLTADEAILDAELVRSV
                     PPAITADTGMDVFTHALESYVSTAHNEFSSALAEKAIEICGVFLLRAYLDGSDMHARK
                     KMHVASCLAGLSFNTAGLGITHSMAHQLGAMFHIPHGRANAMLLPHIVEFNSDINKHS
                     KSQKEYLPAVKRYANVAHILGLSNYNKVMTVRSLVNWIQFMQKEMNIPLTIQELGTIA
                     PEEYFAAIDKMADAALADACTVNNPRVPTKEDIIKIYTKLWSF"
     gene            4504..5586
                     /locus_tag="ACZPD3_04000"
     CDS             4504..5586
                     /locus_tag="ACZPD3_04000"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1415201.1"
                     /translation="MSIYTKRGDRGITDMAHVGNISKSDDRIRLMGEADELNSHIELV
                     KSMLRQSEILQFLERIQKNLDLIAAGVSNPYDRDCKVSEKETAVLEAETDKLEALCEK
                     PVPEKFTGKSRLAAEIDIARTVARRTERCLAQVSVKFGADTESKRFLNRLSDYLYILA
                     RYIEYYSDLSVELSDDHAGALAKTSDNNTDALPEPSDSNTDVLKKTADINEAVIQEVL
                     KRMGIQSRITLDTAKKLIERLEQEAVRRGQRAVIAVCNPEGNPVAVHVMDGAFLVSFD
                     VAMKKAYTAVAVKMSTMELSKIAQPGGTFYGVDKLDGGKIVIFGGGIPLKSGNTIIGG
                     LGISGGTGEEDHSLAEYGQSVLNEIL"
BASE COUNT         1737 a         1013 c         1447 g         1400 t
ORIGIN      
        1 aaggcagata atgagagagg aaccatcttc aatcaggtca agatccgtgt agacgacagc
       61 tttacactgg aaatgcatat tgatacagat gaagcaaatg cagcaaagat tgctacaggc
      121 actaccgtaa caataattaa ataatatcgg gggccaaagg tcaaaaagcc gttcgtgcct
      181 gcacgataag gtttttgaac agaaaccctt aagcgttaat tgggggcaga agtatttgcc
      241 cccaatatta ttatcagaaa caatttggag gctcatatga tcgtaggcaa agttgtagga
      301 agtgtggtat ccacacgaaa gagtgagaag ctgattggaa gtaaatttat gattatcgag
      361 cctgtgcatc atatgaaagg cgacctgagc cagctggttg cgatcgatat gatcggagcc
      421 ggagttggtg aatatgtatt ggtggcacag ggaagcgcag caagaatagg ctgcggcgta
      481 gaaacagcac cggtagatgc cgcaattgtc ggtattattg atgacggtgc aggattggag
      541 taacgccatg gaaatcaaag aattacagga tattatacag cagaatggtg tggtaggtgc
      601 cggcggagcc ggattcccta cttatatgaa gcttacagac aaggccaata ccattcttat
      661 gaactgtgca gagtgtgaac cattactgaa attacacaga cagttattgg aaaaacacgc
      721 atatgaaatt atgaaaacat tccatatgat ccaggagacg gttggtgctt ctgaagcaat
      781 tattggaatt aagaagtctt atgtacagac aattcatgca ttaaatcagc atattgaaga
      841 atttcctggc atgaggctgc atcttctgga cgaagtttat ccgatgggag atgaggttgt
      901 acttatttat gaggcaaccg gaagagtagt acggccggga ggactgccga tcgagcaggg
      961 agtagccgta tttaacgttg aaactgtata caatgtatat cgtgctgtgg aagagaagca
     1021 gccggttaca gacaaatatg tatctgttgt tgcagaagta agtgatcctg ttacagtgag
     1081 agttcccctg ggatgtactc tggaggaagt ggtagcacag gcaggcagca caacagtgaa
     1141 ggatcctgtc tatttcgtag gcggcccgat gatggggcgt atcggaaatg gttcagatcc
     1201 ggtgacaaag acaacaaatg cgattttggt ccttccaaaa gaccatctta ttgtagcgaa
     1261 gaaacagcgg acttcttcta tagatttaaa acgtgcagca tctatctgct gccagtgcaa
     1321 tacctgtaca gatttatgtc cgagacataa tctgggacat ccgatcgatc cggcgaaatt
     1381 tatgagagct gcatcaaata atgatttcag agacctgaat ccatatatag atgcttcctt
     1441 ctgcagttcc tgcggtgtat gtgagatgta ctcatgtccg cagagtcttg caccgagaag
     1501 tcttctcgca gatatgaagg gcggtctgag aaaagcaggt atccgtcctc cgcagggtgt
     1561 acagccgaaa ccggtacagg aatccagaga ataccgtaag gttccggaag aacgtctgat
     1621 ggcgcgactt ggtcttacga gatatgacaa ggatgctcct ctgaaagaag aacttgtaca
     1681 ggttaagaaa gtaaggatat tattgagcca gcacatcgga gcacctgcac aggcagtggt
     1741 caaagccggt gatgaggtaa caagagggca gatgatcgca cagccggcac agggcttaag
     1801 cgttggtatt catgcttctg tcagcggtaa agtaacagaa gtaacagacc gttacattat
     1861 tattgcagta aagtagaaag gacgtgcaca aaatgagtaa agcaatcgga atgattgaat
     1921 ttaaaaccac atctacaggc gttactgcag cagatgctat ggtaaaaaca tctgaggtag
     1981 agatcgtcga ggcacagacc gtatgtcctg gtaaatatat tgcaattatt accggtgact
     2041 taagtgcggt aaaggccgcc gttgatacgg cagtaactac atatgaggat aaatgcattg
     2101 acagctttgt gctgggcaat ccgcatgaat ccattttccc ggcaatttac ggaactactc
     2161 aggtagaaga tatcagtgcg ctgggaattc ttgaaactta tgatgcagct tccattatcg
     2221 aagcagcaga ccaggcagct aagactgcga tcgtagattt gatagaactt cgtattgcca
     2281 aaggtatgtg cggaaaatca tatatgatga tcaccggtga agtttctgcg gtagaggctt
     2341 ccattgacag ggcaaaagaa cttgtagctg ccaagggtat gtacctggat tcttccgtta
     2401 tcgcacatcc tgacagaaga atgatcgaca gtattttata atatccgggg taagaagttt
     2461 ctgaccttga cactcataaa aaatttctca aaaaaagggg gcaaagatat gcttgcgctg
     2521 gagagaagaa atctgattct cgaaaaatta caggcagaaa agcgggtggt ggtcagcgag
     2581 ctgagccagc tttatgacgt ctctgaagag accatacgcc gtgatctgga taaacttgaa
     2641 aaggaaggac ttgcgataaa aagttatggc ggtgctgtga tcaatgagga tgtaagcatt
     2701 gatttgcctt tcaatgtacg taagaaccag aatgttacag gaaagcagaa aatggctgaa
     2761 cttgcagctt cccttgtgaa agacggagat catattttcc tggatgcgag tactacggca
     2821 gtttttgtgg cgaaagcatt aaaggaaaaa gaacgtctta ccgtaattac caattccatg
     2881 gaaatacttc tggagttagc tgatgtatcc gggtggaaca ttatctccac cggaggcgta
     2941 atgaaagaag gatatctggc ttttcttgga tcaaagacag acgaatccat acgttcttat
     3001 tatgtagata aggttatctt ttcgtgtaag gcgctagatc tggaatgggg gattatggaa
     3061 tctcaggaag cttttggctc taccaagaga gcgatgatcg gttccggacg aaagagaatt
     3121 ctggttgttg acagttctaa atttgatcag acagcgtttt ccgtagcagg aagcatgaaa
     3181 gaagtagata ttatagtgac ggacaaagaa ccgacagaac gttggaagaa gcactttgag
     3241 aaatttaatg taaaatgcct atatcctgtc tgataaagaa aataggagta tgatacaatg
     3301 aatacttttg agatgaaaac cgcgatccat tttggggata acgcgttaga cagattaaaa
     3361 gagatcccgt ataaaagggt tctcattata acagatccat ttgtggtaca aagtaaaatg
     3421 atcaatctga tcactgctcc tttaaacagt gcaggtattg aatnnnnnnn nnncggatgc
     3481 acctgtggac aagattgcag aaggggtaaa gaagttcctg gaatatcagc cggaagcagt
     3541 tgttgcagtc ggtggtggtt ctgcgattga ttcttccaag gcaatcagag aatttgcact
     3601 gaagatcaac aattacggaa aagtaggtct gatcgcaatt ccgacgacaa gtggtaccgg
     3661 atcagaagtt acttcttttg cagtagtaaa cgatactgca gcacatgtaa aatatccgct
     3721 gatctcagag agccttacag cagatgaagc aattctggat gcagaacttg taaggagtgt
     3781 tccgccggcc atcacagcgg atactggtat ggacgtgttt acacatgcgc tggaatcata
     3841 cgtaagtact gcacataatg aattctcttc tgcacttgcg gagaaagcga ttgaaatttg
     3901 cggagtattc ctgctgagag catatctgga tggcagtgat atgcatgcgc gtaagaaaat
     3961 gcatgtagct tcctgtctgg ctggtttatc ctttaataca gcaggtcttg gaatcactca
     4021 cagcatggct catcagctgg gagcaatgtt ccatattccg catggaaggg caaatgctat
     4081 gctgcttccg catatcgtag aatttaattc tgatattaat aagcacagta agagccagaa
     4141 agagtatctt ccggctgtaa agagatacgc caatgttgcg cacattctcg gactgagcaa
     4201 ttataataaa gtaatgacgg tccgttctct ggtaaactgg attcagttta tgcagaaaga
     4261 gatgaatatt ccgctgacga ttcaggaact gggaacaatt gcaccggaag aatattttgc
     4321 agcaattgac aagatggcag acgcagcact tgcagatgcc tgcacagtga ataatccgag
     4381 agttccgact aaggaagata ttataaagat ttatacgaaa ttatggtcat tctgaaaatg
     4441 aagacaataa tttccggcag atgctgactg ccgctgaaat cagatacagg agatggagaa
     4501 gagatgagta tttatacaaa aagaggagac agaggaatta cagatatggc tcatgtcggt
     4561 aatatttcca agtctgatga ccgtatccgc cttatgggtg aggctgatga actgaatagt
     4621 catattgaac ttgtaaaaag tatgctccgt cagtcagaaa tacttcagtt tcttgaacgt
     4681 attcaaaaga atcttgatct gatcgcagcg ggagtcagta acccatatga tcgtgactgt
     4741 aaagtcagtg aaaaggaaac agcagtgttg gaggcggaaa cagataaact ggaagcactg
     4801 tgcgaaaagc ctgttccgga gaagtttaca ggaaagagcc gactggctgc agagatagat
     4861 attgcacgta ctgttgccag aagaacagag cgctgccttg cacaggtcag cgtgaagttt
     4921 ggcgcagata cagagagtaa gagatttctt aaccgccttt cggattatct gtatattttg
     4981 gcgagatata tagagtatta ctctgatctt tcagtggaac tgtctgatga tcatgcgggt
     5041 gcattggcga aaacgtctga taataataca gatgcattgc cggaaccgtc cgacagtaat
     5101 acagatgtat taaaaaagac ggcagatatc aatgaagccg tcatccagga ggttcttaaa
     5161 cgaatgggaa ttcagagcag gataacattg gatacagcga agaaactgat cgagagactg
     5221 gagcaggaag ctgtgcgcag agggcagcgt gcggtaatcg cggtctgcaa tccggaagga
     5281 aatccggttg cggtccatgt gatggatgga gcattccttg taagcttcga tgtggcaatg
     5341 aagaaagcct atactgcagt ggcagtgaag atgtctacaa tggaactttc gaagatcgca
     5401 cagcccggag gaacctttta tggcgttgac aagctggatg gtggaaaaat cgttattttc
     5461 ggcggcggta ttccgctgaa gtccggaaat acaattatcg gtggacttgg aatcagcggc
     5521 ggtacaggag aagaagacca tagtctggca gagtatggtc agtctgtgtt aaatgaaata
     5581 ttgtaagatg gatgacaact atttggc
//
LOCUS       DBMDXG010000094         5569 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3629_length_5569_cov_1.296818, whole genome shotgun sequence.
ACCESSION   DBMDXG010000094 DBMDXG010000000
VERSION     DBMDXG010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_5569_cov_1.296818"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            129..2720
                     /gene="clpB"
                     /locus_tag="ACZPD3_04005"
     CDS             129..2720
                     /gene="clpB"
                     /locus_tag="ACZPD3_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR1415202.1"
                     /translation="MNISKFTQKSVQAVQDLEKVAYQFGNQEIEEEHLLYALLEQEDS
                     LILKLIEKMEIDKDYFRNSLNQALDAKVKVSGGELRFGQYLNKALVSAEDEAKAMGDE
                     YVSVEHLFLALLRYPSPSMKKLFQEFGITKERFLQALSTVRGNQRVVSDNPEATYDTL
                     NKYGEDLVEKARNQKLDPVIGRDEEIRNIIRILSRKTKNNPVLIGEPGVGKTAAIEGL
                     AQRIVAEDVPEGLKDKKIFALDMGALVAGAKYRGEFEERLKAVLEEVKKSEGNIILFI
                     DELHLIVGAGKTDGAMDASNMLKPMLARGELHCIGATTLDEYRQYIEKDAALERRFQP
                     VMVDEPTVEDTISILRGLKERYEVFHGVKITDSALVAAATLSHRYITDRFLPDKAIDL
                     VDEACALIKTELDSMPSELDEQRRKIMQLEIEESALKKETDNLSKERLEALQKELAEL
                     RDTFNTQKAQWDNEKHSVEKLQKLREQIEDVNKQIQKAKQNYDLEKAAQLQYGELPKL
                     QQQLEIEEKSVKESDRSLVHEAVTDDEIARIISRWTGIPVTRLTEGERAKLLTLEDQL
                     HKRVVGQDEGVKRVTDAILRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKTLAENLFD
                     DEQNMVRIDMSEYMEKYSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVVLFDEIEKA
                     HPDVFNVLLQVLDDGRITDSQGRTVDFKNTILIMTSNIGSPYLLDGIDENGEIKPEAQ
                     SQVMDDLRGHFRPEFLNRLDEIIMFKPLTKSNIGKIVDLMVGELDKRLADQELSLELT
                     DTAKDQVIENGYDPVYGARPLKRYLQKYVETLAARKILSGDVHAGDTLVLDVQNGEFI
                     VTVKDGN"
     gene            2721..2894
                     /locus_tag="ACZPD3_04010"
     CDS             2721..2894
                     /locus_tag="ACZPD3_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HIR1415203.1"
                     /translation="MIPKDPMILLSYVNTQLRDYYDSLEALCTCRGLKKDELVAKMHS
                     IDYEYDEATNQFI"
     gene            3320..4759
                     /locus_tag="ACZPD3_04015"
     CDS             3320..4759
                     /locus_tag="ACZPD3_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR1415204.1"
                     /translation="MKKLVTVLGAFFAVLLACPLSVFAAEGSKAPSTVPVWLCIPFAG
                     LLLCIAVMPLVKAEWWESHQPLVVALWIILMVVPFAFVYGAGKTAETVLDCIVNDYLT
                     FIILLFGLFCVSGNITMEGDFAGSPRVNVGLLALGTLLASCIGTTGASMLMVRPVIKM
                     NSWRKRKGHIMIFFIFMISNMGGCLTPIGDPPLLMGFMRGVPFFWSLHLFPVLIFNMV
                     ILLFVFYQIDKRSYRKDIANGRKPDIRKPGTQFRLDGLHNIIFLVMIVGAVILSGVLP
                     GMSAFQDAAGNVRGIHLFGEVTLTFPALIEIVIILLAAFLSFKTTDKQIRIRNHFTWG
                     AIQEVAVLFIGIFITMQPALMLLKAVGPNLGVTEPAEMFWATGALSSFLDNTPTYLVF
                     LTTAGTLAFTNGITTTLGTVPTKILSAISCGAVFMGANTYIGNAPNFMVKAISDENGV
                     KMPSFFGYMLWSVAFLIPVFVIDMFVFFL"
     gene            4775..>5569
                     /locus_tag="ACZPD3_04020"
     CDS             4775..>5569
                     /locus_tag="ACZPD3_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1415205.1"
                     /translation="MQDNQEKCKELAIRIYNLDEEVYKCVGSSLGRTFTGQKETTLRH
                     LAALMYTKPDGHLLRSELALISNMARTIRDKSERRRLMIEYDSILKEIESLPDTFGQT
                     DIVDKERISLNNLIARRQNEISENDHLIICISRTQGSGGNDIGFELADQLQINYYDAE
                     IFDQVMKRLQADKNAVSDTGNFEGFDKYRKKKHTDLKTWFREFNRYHGLPKQDAVFFN
                     MSDLICELAKSEDCVIMGRCADAILKNNHIPHISLFISAPFQVRVQH"
BASE COUNT         1631 a         1041 c         1387 g         1510 t
ORIGIN      
        1 gttgaaaaaa aattagcact cacctattga cagtgctaac aacgtgtgtt atattacatg
       61 cagaacaaag aaaacaacat atttctcctt cgggagagat atttctcata caagaggagg
      121 gttagcctat gaatatcagt aaatttacac agaaatccgt acaggctgtc caggatctgg
      181 agaaggttgc ttatcagttt ggcaaccagg agattgaaga ggagcatctt ttatatgctc
      241 tgctggaaca ggaagatagc ctgatcctta aactgatcga gaagatggag atagataagg
      301 attatttcag aaattctctg aatcaggcac tggatgctaa ggttaaggta tccggcggtg
      361 agctccgttt cggacagtac ctgaacaagg ctctggtcag tgcagaggat gaagccaagg
      421 caatgggaga tgaatatgtc tctgtagagc atctcttcct tgctttgttg agatatccaa
      481 gtccaagcat gaagaaactg tttcaggaat ttggcattac taaggagcgt ttcctgcagg
      541 cactttctac agtacgaggc aatcagcgtg tagtcagtga caatccggaa gctacttatg
      601 atactttgaa taaatatggt gaggatttag ttgaaaaggc acgtaaccag aagctggatc
      661 ctgtgatcgg acgtgatgaa gaaatccgta atatcatcag aatcctttcc cgtaagacca
      721 agaacaatcc ggtgcttatc ggtgagcctg gtgtaggtaa aacagcagca atcgagggtc
      781 ttgcacagcg aatcgtggcg gaggatgtgc cggaaggtct gaaggataag aagatttttg
      841 ccttggacat gggtgctctg gtagcaggtg ccaaatacag aggtgagttt gaggaacgtc
      901 tgaaagcagt ccttgaagaa gtaaagaaga gtgaaggaaa catcattctt ttcattgatg
      961 agttacatct gatcgttggt gcaggtaaga ctgacggagc catggatgca agcaatatgc
     1021 tgaagcctat gcttgccaga ggtgagcttc actgtatcgg tgccacaact ctggatgagt
     1081 atcgtcagta tattgagaaa gatgccgctc tggaacgtcg tttccagcct gttatggtag
     1141 atgaacctac agtggaggat accatttcca tccttcgtgg tctgaaggag agatatgagg
     1201 tcttccatgg tgttaagatc acagacagtg cgctggttgc agcagctaca ctttcacata
     1261 gatatatcac agaccgtttt cttcctgata aggccattga cctggtggat gaggcctgtg
     1321 ccctgatcaa gacagagctg gattccatgc cttcagagct ggatgaacag cgccgtaaga
     1381 tcatgcagct ggagattgag gaatctgcac tgaagaaaga gacagataac ctgagtaagg
     1441 aacgtctgga agcacttcag aaggaactgg cagagcttcg agataccttt aatacacaga
     1501 aagcacagtg ggacaatgag aagcattctg tagagaaatt acagaagctt cgtgagcaga
     1561 ttgaggatgt gaataagcag atccagaaag caaaacagaa ctatgatctg gagaaggctg
     1621 cacagttaca gtacggcgaa cttccgaaat tacagcagca gctggaaatc gaagagaaga
     1681 gtgtcaaaga aagtgacaga agtctggttc atgaggcagt tactgatgat gagatcgcaa
     1741 ggatcatttc cagatggacc ggtattcctg taacaagact gacagaaggc gagagagcca
     1801 aactcctcac tctggaagat cagcttcaca agagagttgt aggccaggat gaaggtgtga
     1861 agagggttac agatgcaatc cttcgttcca aagccggcat caaggatccg accaaaccta
     1921 tcggttcctt cctgttcctt ggtcctacag gtgtaggtaa gacagagctt gccaaaactc
     1981 tggcagagaa tctgtttgat gatgaacaga atatggtcag aattgatatg agtgaataca
     2041 tggagaagta ttctgtatcc agactgatcg gagcgcctcc gggatatgta ggatatgagg
     2101 agggcggcca gcttactgag gcagtccgcc gtaaaccata cagtgttgta cttttcgatg
     2161 agattgagaa agcacatccg gatgtgttta acgtactgct gcaggtactg gatgatggcc
     2221 gtatcacaga ttctcaagga cgtacagttg actttaagaa tacaattctt atcatgactt
     2281 ccaacatcgg ttctccgtat ctgttggatg gtattgatga gaatggtgag atcaaacctg
     2341 aggctcagag tcaggttatg gatgacctga gaggccattt ccgtccggaa ttcctgaacc
     2401 gtctggatga aataattatg tttaagccct tgacaaaatc caatattggt aagatagtag
     2461 acttgatggt cggcgagctt gacaaacgtc ttgcagacca ggagctttcc ctggaactga
     2521 cagatacagc caaggatcag gttatcgaga atggctatga cccggtatat ggtgcacgtc
     2581 cgttgaagcg ctatctgcag aaatatgtag agactcttgc ggcgagaaag attttgtccg
     2641 gagatgttca cgcaggtgat actctggtgc tggatgttca gaatggggaa ttcattgtta
     2701 cggtaaagga cggaaactaa atgataccca aagatccaat gattctgctg agttatgtga
     2761 atactcagct cagggattac tatgattccc tggaagcact ctgcacatgc agaggattga
     2821 agaaagacga actggtggcg aagatgcatt ccatcgatta tgagtatgat gaggcaacca
     2881 atcagttcat ataagaaata tgcgaaatgc tctctgacag cgggaattgc tgttggggag
     2941 catttttcgt attgcatata agaaagctcc ctgctttaat tgcccaaaag tgataaactg
     3001 cggagcagtt attgggtcat gtgtttcaaa atcagtacaa ttttaaaaaa tagataaggc
     3061 agcacaaaaa atgtcagtaa taaagattaa atcgttaaaa tataaacgaa aataatcagg
     3121 aaatatgtaa aaaatgtata aaaaaacagg aaataaagta taaatattca ctatccacac
     3181 aattgatttt tgtatgctgt atgatataat tgcatctgta aaaaacaagc gcatattaaa
     3241 taggaagaaa cacgtatatg aaaaaaatct cctgaatgat aatcgcgctg tacgaaaata
     3301 caatatggag gtggcagttt tgaaaaagtt ggtgaccgta ctgggtgctt tttttgcagt
     3361 tcttctggct tgtccattga gtgtatttgc ggcagaaggc agtaaagcac catctacagt
     3421 acctgtatgg ctttgcattc cctttgcagg gcttttgtta tgtattgcag tgatgcctct
     3481 ggtgaaagcg gagtggtggg agtctcatca gccactggtt gttgccttat ggatcattct
     3541 gatggtggtt ccatttgctt tcgtttatgg agccggaaag actgcagaga cagttctgga
     3601 ctgtattgtg aatgattatc ttacattcat cattctgtta ttcggcctgt tctgcgtatc
     3661 aggtaatatt accatggagg gtgatttcgc aggttccccc cgtgttaatg taggtctgct
     3721 tgcactggga actcttctgg caagctgtat cggtaccacc ggtgccagta tgctgatggt
     3781 acgtccggta attaaaatga attcatggag aaagagaaaa ggacatatta tgattttctt
     3841 tatcttcatg atttccaata tgggtggatg tcttaccccg attggagatc cgccgctgtt
     3901 aatgggattt atgcgaggtg ttccattctt ctggagcctg catctgttcc ctgtactgat
     3961 ctttaatatg gtcattctgc tgtttgtatt ttatcagatt gataaacgtt cctacagaaa
     4021 agatattgca aatggccgta aaccggatat ccgtaaaccg ggtactcagt ttcgtctgga
     4081 tggactgcac aatatcattt tccttgtgat gatcgttggc gcggtaattt taagcggtgt
     4141 acttccggga atgtccgcat tccaggatgc tgcaggcaat gtaagaggca ttcatctctt
     4201 tggagaagta accctgacat tcccggctct gatcgaaatc gtgatcattc tgcttgctgc
     4261 ttttctttcc tttaagacaa cagataagca gatccgtatc aggaaccatt ttacctgggg
     4321 tgcgatccag gaggttgcgg ttttattcat cggtattttt atcaccatgc agcctgcatt
     4381 gatgcttctg aaagctgtag gaccgaatct gggagttact gaaccggcag agatgttctg
     4441 ggcaacaggt gcactttcca gtttcctgga taacacacct acttatctgg tattccttac
     4501 aacagccgga acgctggcat ttacaaatgg aatcacaacc acactgggaa cagtacctac
     4561 caagattctg tctgccattt cctgtggtgc ggtattcatg ggtgccaaca cttatatcgg
     4621 aaatgccccg aactttatgg taaaggcaat ctctgatgag aacggtgtga agatgccgtc
     4681 attcttcgga tatatgctct ggtctgtggc atttctgatt ccggtgtttg ttattgatat
     4741 gtttgtattc tttttataag gagggaggat cattatgcag gataatcagg aaaaatgtaa
     4801 agaacttgca atacgcattt ataacctgga cgaagaagtt tataaatgtg tgggttcctc
     4861 tctgggacgt acatttacag gacaaaaaga aacaactctc cgtcatctgg cagcactgat
     4921 gtataccaag ccggacggac atctgctcag aagtgaactg gcgctgatca gtaacatggc
     4981 ccgtaccatc agggataaga gcgagagaag acgtcttatg atagagtatg acagtattct
     5041 gaaagagatt gaaagtcttc cggatacatt tggacagaca gatattgtag ataaagaacg
     5101 tatcagtctg aataatctga tcgcaagacg ccagaatgag atttccgaaa atgatcatct
     5161 gatcatctgt atcagccgta cacagggaag tggcggtaat gatatcggat ttgaactggc
     5221 ggatcagctt cagatcaact attatgatgc agaaattttt gatcaggtta tgaagcgtct
     5281 gcaggcagac aaaaatgcag tcagtgatac tggtaatttt gaagggtttg ataaatacag
     5341 aaagaaaaaa cacacagatc tgaaaacctg gttcagggaa ttcaatcgtt atcatggact
     5401 tccgaagcag gacgcagtat ttttcaatat gagtgatctg atctgtgaac tggcaaagag
     5461 tgaagactgc gtgatcatgg gacgttgcgc agatgcgatt cttaagaata atcatattcc
     5521 tcatatcagt ctgtttatca gtgctccgtt ccaggttcgt gtgcagcac
//
LOCUS       DBMDXG010000095         5491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3682_length_5491_cov_1.604453, whole genome shotgun sequence.
ACCESSION   DBMDXG010000095 DBMDXG010000000
VERSION     DBMDXG010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_5491_cov_1.604453"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="larC"
                     /locus_tag="ACZPD3_04025"
     CDS             <1..1263
                     /gene="larC"
                     /locus_tag="ACZPD3_04025"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR1415206.1"
                     /translation="VLDKVLRSLPISGFETKISRVVKSGIDACDFDVVLDKDHENHDH
                     DMEYLHGHHHEGHERNHAHGTGTVQDHHHHEHRGIKEITCIIEHSAMTENAKKIALRI
                     FEILAEAESKAHNVPVDQVHFHEVGAVDSIVDIVSVAVCLDDLDVTEVIVPVLCEGRG
                     TVRCQHGILPIPVPAVANIVSANHLHLKMTEVEGELVTPTGAAIVAAVKTKDKLPETF
                     EIQKIGIGAGKRQYECPGILRAMIISQSAETDEEKAQTEEFKNPEIRNNPKAENQETK
                     DTIIKMETNIDDCSGEVLGFVMERLMKAGARDVHYVPVFMKKNRPAWVLNVICKEEDM
                     ETLQNIIFEETTTIGIRYSRMERTILPRETRTLPTPWGEVLAKVCTLNGKEQLYPEYE
                     SVAQLSREKEIPFAEIYRYIVLANKDKE"
     gene            complement(1431..3383)
                     /locus_tag="ACZPD3_04030"
     CDS             complement(1431..3383)
                     /locus_tag="ACZPD3_04030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR1415207.1"
                     /translation="MIQKYTYGTPFETDAIVTSIPTSEGTPAYGTISTENGFSFTYDM
                     DDNDVVYGLGESNRGINKRGYVYESNCSDQPNHTEDKISLYGAHNFLVISGKLTFGLF
                     VDYPGKLKFDIGYTLSSQLKITCEDADLYLYVIQGETPYDIVKQFRKIIGRSYIPPKF
                     AFGFGQSRWGYTTADDFRKAADGYRENNIPIDMVYMDIDYMEDYKDFTVNQENFPDFE
                     AYVNEMKEKGIHLVPIIDAGVKVEAGYDVYEEGVEKNYFCKREDGSDFVSAVWPGWTH
                     FPDVLNADARAWFGQKYERLISKGIDGFWNDMNEPAMFCTPEGVAELKEYIKDNFMDN
                     EETSGFVLGAKVKGLANNPEDYKRFYHNVNGQKIRHDKVHNLFGYNMTRAAGEAFEKI
                     APGKRFLMFSRSSYVGMHRYGGIWMGDNKSWWSHILLNLKMLPSLNMCGFLYTGADLG
                     GFGADTTRDLVLRWLALGVFTPLMRNHSALGTREQEAYQFENIEDFRHVIGVRYRLVP
                     YLYSEYMKAALNDDMMFRPLAFDYTDDAFATQVEDQLLLGNEIMIAPVYTQNAKGRYV
                     YLPEEMMFVKFLPDGTISQEILEKGHHYVEVALNEVPLFIRKGKAIPVADVAQTVKDI
                     DPATIRMIGYEGAEYDRYDDDGVSTL"
     gene            3764..5149
                     /locus_tag="ACZPD3_04035"
     CDS             3764..5149
                     /locus_tag="ACZPD3_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR1415208.1"
                     /translation="MLQTIESINNVVNNFIWGVPAMICIIGVGLYLSLRTGFVQIRKF
                     PYALKTTLGRIFKKKEASDGSMTPFQAVCTALAGTVGTGNIAGVAGAIAIGGPGAVFW
                     MWVSALLGMCTKFTEVTLAVHFRERNRHGDYVGGPMYYIKNGLGKNWRFLAVLYSAFG
                     VLTVFGTGNATQVNTITTAIDTALINFNVISESSTGRLNLILGIVITLLVGMVLLGGI
                     KRIGSVSEKLVPFMALFYIVLALGVVILNIGRVPAVFHDIVYGAFNPSAVTGGVIGSF
                     FLSMKKGVSRGIFSNEAGLGTGSIAHACADTSKPVKQGMFGIFEVFADTIVICTLTAL
                     VILVSGVPVNYGAAAGAELTISGFTATYGGWVSIFTAVAMCCFAFSTIIGWGLYGARC
                     IEFLFSAKVIRPFMIAYSLVAILGATVNLGLLWSIAETFNGLMAIPNLIGVFLLSGTA
                     ITLTKEYFAQK"
     gene            complement(5305..>5491)
                     /locus_tag="ACZPD3_04040"
     CDS             complement(5305..>5491)
                     /locus_tag="ACZPD3_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415209.1"
                     /translation="LDHHIFMVKIFYACFLLMKYKREVNRISSMSTHFPPEIVEIYIH
                     IIREKPQKSESGWLWIK"
BASE COUNT         1501 a         1075 c         1280 g         1635 t
ORIGIN      
        1 gtgttggaca aggtgctgag aagccttccg atatcaggat ttgagacaaa gatcagcaga
       61 gttgtgaagt ccggaataga tgcatgtgat tttgatgttg tgctggataa agatcatgaa
      121 aatcatgacc atgatatgga atatctgcat ggacatcatc atgaaggtca tgagcgcaat
      181 cacgcccatg gcacaggaac agtgcaagac catcaccatc acgaacaccg tggaataaaa
      241 gaaattacct gtattataga gcatagtgca atgactgaga atgcaaagaa gattgcattg
      301 cgtatatttg aaattcttgc agaggcagag agtaaagcac acaatgttcc tgtggatcag
      361 gtgcattttc atgaggtggg agcagtggac tctattgtgg atattgtgtc tgtggcagtc
      421 tgtctggatg atctggatgt tacagaggtg atcgttcctg ttctctgcga gggacgggga
      481 actgtccgtt gtcagcatgg catcctgcca atcccggttc cggcagttgc aaatatagta
      541 agtgccaacc atttacatct gaaaatgaca gaggtagagg gtgagcttgt aacgcctaca
      601 ggtgcagcga tagtggcagc agtgaaaaca aaagataaac ttccggaaac atttgaaatc
      661 cagaagattg gaatcggcgc aggtaaacgc cagtatgagt gtccgggaat cctaagggca
      721 atgattatct cacagagcgc agaaacagat gaagaaaagg ctcagacaga agaattcaaa
      781 aacccggaaa tcagaaataa tccgaaagct gaaaatcagg aaaccaaaga tacgattatc
      841 aagatggaga caaacattga tgactgcagt ggagaagttc tgggatttgt catggagcgt
      901 ctaatgaaag ccggtgccag agatgtgcat tatgtgcctg tttttatgaa aaaaaacaga
      961 cctgcatggg ttctgaatgt gatctgtaag gaagaagata tggaaacgct tcagaatatt
     1021 atttttgaag aaactactac gattggaatt cgttatagca gaatggaacg tacgattctt
     1081 cccagagaaa cgagaaccct tccgactcca tggggagaag ttctggcaaa ggtatgcact
     1141 ttgaatggaa aagagcagct ttatccggaa tatgagagtg ttgcacagct gagcagagaa
     1201 aaagagatcc catttgcaga aatttaccgt tatattgttt tggcaaataa agataaagaa
     1261 taacaatgta aaagaaaaca gtcacatact tgagtgaata tctcaacatt catcagatgt
     1321 atgtgactgt ttctttttat tatttttatg aggtgacttc tggttcagtt ctatctgcta
     1381 cctggtactt tttgcaattc aggcagggaa agttgtctga ttcggttaaa ttacagagta
     1441 cttacaccgt catcatcata acggtcatat tcagctccct catatccgat catgcggatc
     1501 gtagccggat cgatatcctt tactgtctgt gcaacatcag caaccgggat tgcttttcct
     1561 ttgcgaataa acagtggaac ttcattcagg gcaacttcta catagtgatg tcctttttca
     1621 agaatctcct gagaaattgt tccgtcaggc aggaatttta cgaacatcat ttcttccggc
     1681 agatatacat agcggccttt tgcattctgt gtgtaaactg gtgcgatcat gatttcgttt
     1741 ccaaggagaa gctggtcctc tacctgagtt gcgaatgcat cgtctgtgta gtcaaatgcc
     1801 agcggacgga acatcatgtc gtcgtttaat gctgctttca tgtattcact gtacagatat
     1861 ggaacaaggc gatatctgac accgatcaca tgacggaagt cttcgatatt ttcaaactgg
     1921 taagcttcct gttctctggt tcccagagcg gagtggtttc tcataagagg tgtgaataca
     1981 cccagtgcca gccagcgcag taccaggtca cgtgtggtat ctgcaccgaa accgcccaga
     2041 tctgcacctg tgtaaaggaa accgcacata ttcagggatg gaagcatctt taagttcaga
     2101 aggatgtgtg accaccagga tttattgtct cccatccaga ttccgccgta tctgtgcatt
     2161 cccacatagg aggaacggga gaacatcagg aaacgtttgc caggtgcgat tttttcaaat
     2221 gcttcacctg ctgcacgggt catgttatag ccaaacaggt tatgtacctt gtcgtggcgg
     2281 atcttctgac cgtttacatt gtgatagaag cgtttgtaat cttccggatt atttgcaagt
     2341 ccttttacct ttgcgccaag aacgaatcca gatgtttctt cattgtccat gaagttgtct
     2401 ttgatgtatt cttttaattc agcaacgcct tccggtgtgc agaacattgc aggctcgttc
     2461 atgtcattcc agaaaccgtc aattcctttg gaaatgagac gttcatattt ctgtccgaac
     2521 catgctctgg catctgcgtt caggacatcc gggaaatgag tccagcccgg ccatacagcg
     2581 gaaacgaaat cactgccgtc ttcacgtttg cagaagtagt tcttctcaac gccttcttca
     2641 taaacatcat agccagcctc taccttgaca cctgcatcga tgatcgggac aaggtggatg
     2701 cctttttctt tcatttcatt gacataggcc tcaaagtcag ggaagttttc ctgattgact
     2761 gtgaaatctt tgtagtcttc catgtaatcg atatccatgt ataccatatc gattggaata
     2821 ttgttttcac gatatccgtc agcagctttt ctgaagtcat cagcagttgt atatccccag
     2881 cggctctgac cgaaaccaaa tgcgaattta ggcgggatat agcttcttcc gatgatttta
     2941 cggaactgtt ttacgatatc gtaaggggtt tcaccctgga tcacatacag ataaagatct
     3001 gcatcctcac aggtgatctt taactgggaa gagagtgtgt aaccgatatc aaacttcagt
     3061 tttccgggat aatcaacgaa gagaccgaaa gtcagttttc cggagattac gaggaagttg
     3121 tgtgctccgt agagggagat tttatcttct gtatggtttg gctggtcgga acagttgctc
     3181 tcataaacgt atccacgttt gttgattccg cggtttgatt cgcccagacc atagacaaca
     3241 tcgttgtcat ccatgtcata agtaaaagag aagccgttct ctgtggagat agttccgtat
     3301 gcaggagttc cctcagaagt cgggatagaa gttacgatgg catcggtttc aaatggggtc
     3361 ccgtaagtat atttctgaat cattttgtcg tgtcctcctt aggttgaaaa tggtcaagcg
     3421 gataaaatca tccgctttgc tacgcagcgc tacaccgaat aactgaaacg cttaagtact
     3481 gttagaaaat acataggcgt tttccttgat ttcatattta caataataga ggtttgggtg
     3541 aggaaatgct actgaaatta tgacaaattt atacgttatt ttgacttttg ctcaaactgt
     3601 gaagcagtga tatgggtatg gcaagcagtg gagcggctga tcgtatctgt ttgcgctgtt
     3661 aacttattgt gctttaatgc tttatcttaa tagaattttt ctgtacatac ggtgaaaaaa
     3721 gtgctaaact gttattatta cacgaaagaa aggtttattt aacatgcttc agacaattga
     3781 atccatcaat aacgtcgtaa ataattttat ctggggtgtc ccggctatga tctgtattat
     3841 cggcgtaggg ctttacctga gcctgcggac cggttttgtc cagatccgca aattcccgta
     3901 tgcgcttaaa accacactgg gaagaatttt taagaaaaaa gaagcctctg acggttccat
     3961 gacacctttc caggcagtct gtactgcact ggcaggtaca gtaggtactg gaaatatcgc
     4021 cggtgttgcc ggagctatcg ccatcggcgg accgggagct gtattctgga tgtgggtttc
     4081 cgcgctcctt ggaatgtgca cgaaatttac agaggttacc ctggcagtac atttccgtga
     4141 gagaaacaga catggggatt atgtaggcgg acctatgtat tatattaaga acggattggg
     4201 aaagaattgg agatttcttg cagttcttta ttctgcattc ggtgtactga ctgtatttgg
     4261 aacaggtaat gctactcagg taaatactat tacaacagca attgatacag ctcttattaa
     4321 ttttaatgtg atctcagagt cttctacagg cagactgaat ctgattcttg gaattgtgat
     4381 cacgctgctg gttggtatgg tactgctggg tggtatcaag cgaattggaa gtgtttccga
     4441 gaagctggtg ccgtttatgg cattgtttta tattgtactt gcattgggcg ttgtgatctt
     4501 aaatatcgga agagttccgg cagttttcca tgatattgtt tatggagcct ttaatccgtc
     4561 agcagtgaca ggcggtgtga tcggaagctt tttcctcagt atgaaaaagg gtgtttccag
     4621 aggtattttc tccaatgaag ccggacttgg tacagggtct atcgctcatg cctgtgcaga
     4681 tacaagcaag cctgtaaaac agggtatgtt tggtatcttt gaggtatttg cagatacgat
     4741 cgtgatctgt actctgacag cacttgtgat ccttgtaagc ggcgtgccgg taaattacgg
     4801 tgcagcggca ggtgcagaac ttacaatttc cggatttact gctacatacg gaggctgggt
     4861 ttctatcttt acagcagttg ccatgtgttg ctttgcgttc tcaacgatta tcggatgggg
     4921 actttacgga gcacgctgca ttgaattctt attctctgca aaagttatcc gcccatttat
     4981 gattgcctat tcattagtgg caatccttgg tgcaactgta aatctgggac ttctctggag
     5041 cattgctgag acattcaacg gtctcatggc aatccccaac ttaattggtg tattcctgct
     5101 ttcaggaacc gccattacac ttacaaaaga gtatttcgca cagaaatgat aaaaacagtc
     5161 tggagagttt tcttcaggct gtttattagt ttacctgatg cttgatgtta ggttattgtt
     5221 cagacccttg catagggcat tagtaaaaat gacgaatttt aaattttttt attgatgtta
     5281 tgtactgttt tttatccgtc atttttactt tatccacaac cacccggatt cgcttttttg
     5341 cggcttttcc cttataatgt gtatatagat ttcaacgatt tcaggaggga agtgagtgga
     5401 cattgaactt attctattca cttccctttt gtatttcatc aggagaaagc aggcatagaa
     5461 tatttttacc ataaaaatgt gatgatccaa c
//
LOCUS       DBMDXG010000096         5481 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3695_length_5481_cov_1.322119, whole genome shotgun sequence.
ACCESSION   DBMDXG010000096 DBMDXG010000000
VERSION     DBMDXG010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_5481_cov_1.322119"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="ACZPD3_04045"
     CDS             complement(<1..190)
                     /locus_tag="ACZPD3_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415210.1"
                     /translation="MKRKRFSRLLFLMLTIMITVTFYQPAQAASGKYTGTYTKTWSVS
                     SNMTVTIKPSYSVIVNKVT"
     gene            complement(322..735)
                     /locus_tag="ACZPD3_04050"
     CDS             complement(322..735)
                     /locus_tag="ACZPD3_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014626.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HIR1415211.1"
                     /translation="MCSRYYIDPGMTEDISRVVQNIDGRIWLAQGDTRPTDVAPVIGQ
                     SEHGLELGICRWGYPLSKSKGLVINARVETVLDKPSFQNGILYHRLLIPAGGFYEWNS
                     LKEKSIFTRLDSSVLYMAGFCDWFDNSKMPVLLQA"
     gene            complement(892..1647)
                     /locus_tag="ACZPD3_04055"
     CDS             complement(892..1647)
                     /locus_tag="ACZPD3_04055"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1415212.1"
                     /translation="MKFENKVAIITGSTRGIGRATAELFAKEGAKVIVVGTKEELGES
                     CVNEIKAAGGEAIFCKTDVTSDESLDNLVKTALDAYGKIDILVNNAGVGGTTANMDQI
                     TMDEWNTVLATNLTAPFVLCKKLIPIMEKQGAGTIVNVASMAATAAGRGGLAYTSAKH
                     GLLGLTRQMSLDHGRTGVRINAVLPGPIATDMIARVLAIPQHPVTMKIGMSPAKRPGE
                     PIEVAQAIAFLASDEASFIHGAALAVDGGYTIF"
     gene            complement(1661..4471)
                     /locus_tag="ACZPD3_04060"
     CDS             complement(1661..4471)
                     /locus_tag="ACZPD3_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1415213.1"
                     /translation="MYQEIFKQGKIGNITLKNRLVMSPMGTSLAEMDGSPSEDMIAFY
                     EARAIGGAALIIPEIARVNDLHGAGMMRQLSVSKDRHIEGLAKLAETVHKYGTKIFIQ
                     LHHPGRETVTALTGGPVVSASAIPCKYLQQETRALSTEEVKALIQQFISGAVRVKKAG
                     CDGVELHAAHGYLLQQFLSPYTNKREDEYGGSFENRLRMITEIINGIRVQCGPDFPIG
                     VRLSVEEFLDKTGVTEDYIHIQDGVKIAMALEKCGIDFIDVSVGLYETGSVCVEPISF
                     PQGWRKDLIKAVKDHVSIPVIGVSCIREPQVAESFLEGGIVDFVSMGRSWLADEQWGL
                     KVLEGRENEICKCINCLRCFESLNAWMGAGIPAECAVNPRACRERLYGNAEYDTQEHK
                     VVVVGGGPCGMIAAKTLAERGMKVTLVDRQSELGGTINLAKKPPFKERMGWIADYYNV
                     EFEKLGVELKLDMEATADKIAEMNPDAVLVATGSKSVIPGSIPGITGENVYTIEDILS
                     GKAGLKNKKVMIIGAGVTGLETAEYLCHEGNTVVLADMLDKVAPNANHTNVADVCGRL
                     KEYNAQFMMAYALKEIKKDGVVLERLNDKINVEVAADAVVLSLGFRPDNHLVEELKEK
                     GIHAQAIGNAVKDGTIAPASRSGFEAARKLFKTSVKTPSFITAPEEMPNFGKISLMKN
                     QEGIYLAYLTDPAAIAKVLPAPLKPFSVPVVTVSVCHVKEPTFADDYYEAILGVYCTY
                     GTQLGLYPIGLVLGGTGAEMAVQCGRDNGSIPKKLGSEFVIRRNNDHVTAQVCRRGTE
                     LVNIDLKIGEYNNAMTGMLYQFPEAGKKTYGGGFYFHLDREPDKEGKSHFQNGALLQN
                     LCEYNYHSWEPGFAAIKLQSSIDDPWGELPIRTVIGGAYSSNDLMVHKLNLCEEIDAD
                     VLAPYLLTARYDRTAFMETGRR"
     gene            4566..>5481
                     /locus_tag="ACZPD3_04065"
     CDS             4566..>5481
                     /locus_tag="ACZPD3_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415214.1"
                     /translation="MCIYAQCTIINNNLQNTVHFSQIIVKRSMKMLFSDLIDYLKNEK
                     TFNIILNCSTDLHEIRDVELLDGTQTGYATDIVYFGYPDVLSPDMQPSQCILVRGENY
                     LFPYSCTDRATVQAKELFAVFNAAKQFLQTHEQVSLYEELVRKGHSSHSLDEVINSAS
                     IKLGYSLVFCDKKFRILSYSTSIPVTDKLWKKNIERGYCTYDFIKNVMSLEAVQGASA
                     SIEAVEVSCPESPYRKCSSKVFLNGVQVGFVLMIENNIPFTAEHLAAMSDVSRAISSV
                     VGHYHPELFQYTSADQQLLEALLIGTPAD"
BASE COUNT         1488 a         1263 c         1038 g         1692 t
ORIGIN      
        1 tggtaacctt atttacaatg acggaatagc tcggcttgat ggtgaccgtc atattgcttg
       61 aaacagacca tgtttttgtg taggtcccgg tgtatttacc ggaagctgct tgggctggct
      121 ggtaaaacgt aacagtaatc atgatagtca gcattaagaa tagtaatctg gaaaaccttt
      181 ttctcttcat ggtaatcaaa tcctccttta tgtttataaa catattgtac tactggattt
      241 atatggaaac aaggacgaaa tacggatttg gaagttttaa aaaccttgac cagggcgaat
      301 atgaaatcac agaaattaag actaagcctg cagaagtaca ggcattttgc tattatcaaa
      361 ccagtcacag aaccctgcca tgtacagcac agaggaatcc agtctggtaa agatactctt
      421 ttctttcagg gaattccatt cgtagaaacc gcctgccgga atcaacagcc ggtgatataa
      481 gatcccgttt tgaaaagaag gtttatccaa aacagtttct acccttgcat tgatgaccag
      541 acccttgctt tttgacaggg gatatcccca tctgcatata cccagttcca gtccgtgttc
      601 tgactgtcca atgactggtg cgacatcggt cggacgggta tctccttgtg ccagccagat
      661 cctcccatct atgttctgta ccactctgga aatatcttcc gtcataccgg gatcaatgta
      721 atatctgctg cacatagttc tctcctattt aagaattcat gtactgacag tacgttagac
      781 ttggatgatt ttccttaggg tactatattt tgatttatct ctgcagtcag atcctacgta
      841 ctgaaaaaag gagtccaaat gactcctttt tctaatgcat atcaattatt tttaaaatat
      901 cgtatatccg ccatctacag ccaaagctgc tccatggatg aaactagctt cgtctgatgc
      961 aaggaatgca atggcctgtg ctacttcgat aggttctcca ggacgttttg ccggactcat
     1021 tccaatcttc attgttaccg gatgctgtgg aattgcaagc acacgtgcaa tcatatctgt
     1081 tgcaatcggt cctggaagaa cagcatttat acgcactcct gtacgtccat gatccaatga
     1141 catctgacgg gtgagtccaa gaaggccatg tttagcagaa gtgtaagcaa gccctccgcg
     1201 gccagctgcg gttgctgcca tggaagctac attgacgatg gttccagctc cttgtttttc
     1261 cataataggg attagtttct tgcaaagaac aaatggtgct gtgagatttg ttgcaagaac
     1321 agtgttccat tcatccattg tgatctgatc catattggca gttgttccgc ctactcctgc
     1381 attattaacg agaatgtcta tttttccgta ggcgtccaga gctgttttaa ccaggttatc
     1441 aagactttca tcagatgtca cgtctgtttt acagaaaatt gcttctcctc cggctgcctt
     1501 gatttcattc acacagcttt ctcccagttc ttcctttgtt ccaacaacta tgacttttgc
     1561 tccttctttt gcaaagagtt ctgcggttgc tcttccgatt cctcttgttg aaccggtaat
     1621 gattgctact ttgttttcaa acttcatggc aatgccctcc ttatcttctt cctgtttcca
     1681 tgaatgctgt tctgtcatac cgtgcggtaa gcagatacgg tgccagaaca tctgcgtcaa
     1741 tctcttcaca taaattcagt ttatgaacca tcagatcatt tgaggaatac gcacctccaa
     1801 tgactgttct gattggaagt tctccccatg gatcatcaat ggacgattgt aatttaatcg
     1861 ctgcaaaacc aggctcccat gaatggtaat tgtattcaca gagattctga agcagagcac
     1921 cattctggaa atgtgatttt ccttctttat ccggttcgcg gtcaaggtgg aagtagaaac
     1981 caccgccata tgtcttttta ccggcttctg ggaactgata cagcattccg gtcattgcgt
     2041 tattatattc accaatcttg agatcaatat ttacaagttc agtaccacga cggcagactt
     2101 gagcggtcac atgatcattg tttcgacgga ttacaaattc agatcccagt ttctttggaa
     2161 ttgatccatt gtcacgtcca cactgaactg ccatttctgc accggttccc ccaagtacaa
     2221 gtcctattgg atataatcca agctgtgttc catatgtaca gtaaacacca aggattgctt
     2281 cataataatc atcagcaaag gtaggctcct taacatggca cacagatacc gtaactacag
     2341 gcacggaaaa tggttttaac ggtgcaggca gaacctttgc aatcgccgca ggatcggtga
     2401 ggtatgcaag atatattccc tcctgatttt tcataagaga tatcttaccg aaattaggca
     2461 tttcctccgg agcagtgata aagcttggag ttttgacaga tgtcttaaac aatttccttg
     2521 ctgcttcaaa accacttctg gatgcaggag caatcgtacc gtctttcaca gcatttccga
     2581 tagcctgagc atggattcct ttttctttta attcttcaac aagatggttg tccggacgga
     2641 aaccaaggga aagaacaaca gcatctgcag caacttccac atttatctta tcgttcagtc
     2701 tttcaagtac aactccatct tttttgatct ctttaagagc atatgccatc atgaactggg
     2761 cattgtattc tttcaatctt ccacatacat ctgcaacatt tgtatgattt gcattaggag
     2821 caactttgtc aagcatatcc gcaagtacaa cggtatttcc ttcgtgacag agatattctg
     2881 ctgtttcaag tcccgtaaca cctgctccga tgatcattac ttttttattc ttcagtccgg
     2941 cttttccgga aagaatatct tcgattgtat atacattttc tccggttatt ccgggaatag
     3001 atcctggaat gacagattta gaacctgttg ctacaagtac cgcgtccgga ttcatttctg
     3061 caattttatc cgcagtcgct tccatatcaa gttttaattc aactccaagt ttctcaaatt
     3121 caacattata ataatcagca atccagccca tacgttcttt gaaaggaggc ttctttgcaa
     3181 ggtttatcgt acctccaagc tcactctggc ggtcaaccaa tgttaccttc atgccgcgtt
     3241 cagcaagtgt tttagcagcg atcataccac atggtccgcc acccacaact actaccttat
     3301 gttcctgagt gtcatattct gcatttccat aaagacgttc gcggcatgca cgtggattaa
     3361 ctgcacattc cgcaggaata cccgcgccca tccatgcatt caggctctca aagcaacgca
     3421 gacagttgat acatttacaa atttcattct ctcttccttc aagtaccttg agtccccact
     3481 gttcgtctgc gagccagctt cttcccatgg agacaaaatc aacaatacct ccttccagaa
     3541 aactctcagc tacctgtggt tcacgtatgc aggaaactcc tatcactgga atagaaacgt
     3601 gatcttttac ggccttgatc agatcttttc tccaaccctg ggggaaggag atcggctcta
     3661 cacatacact tcctgtctcg tataaaccaa cagacacatc aataaaatca atcccgcatt
     3721 tctcaagcgc cattgcaatc ttaactccat cctggatatg gatataatcc tctgtcactc
     3781 ctgttttatc cagaaattct tcaacgctta aacggacacc gatcggaaag tccggtccac
     3841 actgtacacg gattccattt atgatctcgg tgatcatacg aagtctgttt tcaaaggaac
     3901 caccatactc atcttcgcgt ttatttgtgt aaggggaaag aaactgttgt aatagatacc
     3961 catgagccgc atgaagctct actccatcgc aacccgcttt ttttacccgt acagcacctg
     4021 agataaactg ttggatcaaa gcttttactt cttctgttga aagagccctt gtttcttgct
     4081 gaagatattt gcacggtatt gctgaagcag atactaccgg accgcctgta agagcagtta
     4141 ctgtttcacg tccagggtga tgcagctgga tgaatatctt tgtcccatat ttatgtactg
     4201 tttctgcaag ctttgcaaga ccttcgatat gtctgtcttt tgaaacagac agctgacgca
     4261 tcatccctgc accatgcaga tcgtttaccc ttgcaatctc cggaataata agcgctgcgc
     4321 caccaattgc acgtgcctca tagaatgcaa tcatgtcttc cgatggagac ccatccatct
     4381 ccgccaggct tgtacccatc ggtgacatta caagccggtt tttaagtgta atatttccaa
     4441 tttttccctg tttaaatatt tcctgataca tgtacaccct cctttacttg tgtctcttga
     4501 ttattatggc ttaaaaataa cattttcatt gttaaaaaaa caacggcata aatataaaaa
     4561 agacattgtg tatttatgca caatgcacta taattaataa taatctgcaa aacactgtgc
     4621 atttttcaca aataattgta aagaggagta tgaagatgct gttttccgat cttattgatt
     4681 atttaaaaaa tgaaaaaact tttaacataa tactaaattg ttctactgat cttcatgaaa
     4741 tacgagatgt agagctgctg gatggcactc aaacaggata cgccacagat attgtctatt
     4801 ttggttatcc agatgtcttg tctcctgata tgcagccttc tcaatgtatc cttgttcgtg
     4861 gagaaaacta tctttttcct tattcctgca cggatcgtgc aacagtacag gcaaaagagc
     4921 tttttgcggt ttttaatgca gcaaaacagt ttcttcagac acatgaacag gttagtttat
     4981 atgaagaact ggttcgaaaa ggccactctt cccatagcct ggatgaagtg attaactctg
     5041 catccatcaa attaggatat tctttggtat tttgcgacaa gaaattccgt atcctgtcat
     5101 attctacatc aattccggtt acggataaat tatggaaaaa gaatattgaa cgtggatatt
     5161 gtacatacga tttcattaaa aatgtaatgt cattggaagc tgtccagggg gcttcagcca
     5221 gcattgaggc tgttgaagta tcttgcccgg aatcaccata tcgtaaatgt agttcgaaag
     5281 tgtttttaaa cggtgttcag gttggttttg ttctgatgat agaaaacaat atccctttta
     5341 ccgcagaaca cttagcagct atgagtgatg taagccgggc aatcagctcc gtagttggac
     5401 attatcatcc tgaactgttt cagtacacca gtgcggacca gcagcttctt gaagccttgc
     5461 tgattggaac tccggctgat a
//
LOCUS       DBMDXG010000097         5467 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3702_length_5467_cov_2.202013, whole genome shotgun sequence.
ACCESSION   DBMDXG010000097 DBMDXG010000000
VERSION     DBMDXG010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3702_length_5467_cov_2.202013"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="ACZPD3_04070"
     CDS             <1..366
                     /locus_tag="ACZPD3_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415215.1"
                     /translation="FHDSCIKEMHYLSGAYVNENLDMYPVNDRRILRVIIQRQYEEDS
                     MIEMEFQGLKYLKLFPADERYSCEILDSNIILKEDCIIWSDCEDKTELEDGDTGTLVC
                     ASKLRWRSIFGYMGEKNYW"
     gene            695..1162
                     /locus_tag="ACZPD3_04075"
     CDS             695..1162
                     /locus_tag="ACZPD3_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415216.1"
                     /translation="MKNKKERDWKFIIGMIVLLAIFAVMGGAFWNMVGKQAQIVREEE
                     QKEEANAISAIYIETGEFLKTGVFVDLNNGTIFSADIPAEGIYNKKGKLISDDVLENG
                     DKVKIYGDGIMLESFPGQYPGVTKIQRTGRASLEETQEYEDQVTGMMKPVAVQ"
     gene            1182..1550
                     /locus_tag="ACZPD3_04080"
     CDS             1182..1550
                     /locus_tag="ACZPD3_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1415217.1"
                     /translation="MKYVRQFWIILLISAMGEALHVLIPLPVPASVYGLVIMLIALGT
                     HIIRLEQVKEAAEFLIEIMPVMFIPAGVGLLTAWGILKPVCVPIILITVITTVVVMIV
                     TGRVTQAVIRMDRKKGQKRS"
     gene            1547..2239
                     /locus_tag="ACZPD3_04085"
     CDS             1547..2239
                     /locus_tag="ACZPD3_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1415218.1"
                     /translation="MTQFMSNSLFFGAAISLIAYEAGLLLKRKFKMAIFNPLLIAIIA
                     VIAVLCLLHIDYDTYNQSGQYISYLLTPATVCLAVPLYQQMELLKKNLKAVIIGIVSG
                     VLASLVSVLILAKLFSLSHEQYVTLLPKSITTAIGMGVSEELGGIVTITVAVIIITGV
                     LGNMLAETVIKLARIEEPIAKGLALGTSAHAIGTAKAMELGEIEGAMSSLAIAVAGLL
                     TVVGASVFAQFM"
     gene            2576..2797
                     /locus_tag="ACZPD3_04090"
     CDS             2576..2797
                     /locus_tag="ACZPD3_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415219.1"
                     /translation="MMNAVNTTQRMRRKERHKFSRTMAVILGLIVTLEVMVAGGMIFN
                     IDFHSADMVAVVMGFMVLYAGIVAILTDN"
     gene            2975..3943
                     /locus_tag="ACZPD3_04095"
     CDS             2975..3943
                     /locus_tag="ACZPD3_04095"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR1415220.1"
                     /translation="MTVKSSLLEMLEKNKGEVLSGESIAGELGCTRAAVWKAVKSLRE
                     EGYHIEAGPNKGYMLAKDTNRLSQEGIRLLLDDPKVKIDIYDELESTNQTAKKEAMMG
                     EAGHGAFVIARSQTAGRGRRGREFYSPADTGLYMSVILKPQGTIHDSLLITTAAAVAV
                     YRAVAQLCGIQLDIKWVNDLFYKGKKVCGILTEAVTDFESGNIEFVVVGMGLNLYLDQ
                     ENLPGELRNIAGALYETKEDAEQTDRNKLTATIVNELRKETADLKLSPDYVTHNMIPG
                     HQITITDGNSSRQAFALEICRDGRLKVREEDGQETILSFGEVSVSM"
     gene            complement(4007..4771)
                     /locus_tag="ACZPD3_04100"
     CDS             complement(4007..4771)
                     /locus_tag="ACZPD3_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415221.1"
                     /translation="MQKQKRGFWLFIFSLIPGAGEMYMGFKKQGISIMFLFWGVFAIG
                     ACTGMDWLVFLIPIIWFYSFFNVHNLKSLSEEEFYSIEDSYVLHMDELAGDISSLLKH
                     HGKITAILLIFLGASILWNTLVDFLYMILPGYLADVIGRFTYHLPQLVIAAAIIFAGI
                     YILTRKKDALDEEQPDSFTEEEHYWTPYRPYQQNISDITTDAAKNTTDTTNPDCCTDT
                     DVTDNTTTGNPVSLKKDSAAEVSQKDASSQTTNSES"
     gene            complement(4776..5270)
                     /locus_tag="ACZPD3_04105"
     CDS             complement(4776..5270)
                     /locus_tag="ACZPD3_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415222.1"
                     /translation="MHINKKDLIEFDNGNMNTQEVIEFLSHLDHCDFCLDQMMEQEDS
                     STDTAPSYLMEQILDKADSGEVQLARAANKASRKIRLLHYSLQTAAGVMVALLLLFSI
                     PNLDFTSLHQDTFSQTDRILPEHENGRLYQFTRELGQNIGDGTGSFIKYISDFSGKLM
                     NGGK"
     gene            complement(5271..>5467)
                     /locus_tag="ACZPD3_04110"
     CDS             complement(5271..>5467)
                     /locus_tag="ACZPD3_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1415223.1"
                     /translation="KEKLINAAEKAGHKIHVETQGVIGTEDPLTEEEIAQQTGKNPKT
                     IQTQLYRARAMLKKMLERGD"
BASE COUNT         1658 a          970 c         1317 g         1522 t
ORIGIN      
        1 ttccatgata gctgtatcaa agagatgcat tatctgagcg gagcatatgt taatgaaaac
       61 ctggatatgt atcctgtaaa cgatcgcagg attttgagag taataataca acggcagtat
      121 gaagaagatt caatgattga aatggaattt cagggattaa aatacttaaa gttatttccg
      181 gcagacgaac gctattcatg cgaaatactc gattccaata taattctgaa agaggattgt
      241 atcatctgga gcgactgcga agataaaaca gagctggaag atggagacac aggaactttg
      301 gtatgtgcat ctaaacttcg atggcgttcg attttcggat atatgggaga gaagaattat
      361 tggtaggtgt gccctttccc acattttttt tgacccaagg ggtgtgtaac agcacagtct
      421 ctacttcaat aatcctttgg ataaagcctt tataatttat gtaaagtata tcgtatttat
      481 tctttttcgt aaagaagtct ggatggaagc actgcttacc acagtataat atcagtataa
      541 tatatagaag aaaaagtttt tgcagaaaag aatcaggatt ctgtaaaaac ttttttttta
      601 cgcagaaaca accttgcaat ttgtgatgaa tacgctataa taaaaaaacg taatgaacca
      661 agattggtta gaccaatata aaggagttga atatttgaaa aataaaaaag aacgagactg
      721 gaagtttatc atcggtatga ttgttctgct cgccattttt gctgtaatgg gaggtgcttt
      781 ctggaatatg gtaggaaagc aggcacagat agtcagggag gaagagcaga aagaggaagc
      841 aaatgctatt tctgcaatct atatcgagac aggagaattt ctgaagaccg gagtttttgt
      901 ggatctgaac aatggaacta ttttcagcgc agacataccg gcagagggca tttacaataa
      961 aaaaggaaaa ttaatttccg atgatgtatt ggaaaatggg gataaagtaa agatatacgg
     1021 cgatgggatc atgctggaat ctttcccggg acagtatccg ggcgtgacaa agatccagag
     1081 aaccggtcga gcatctctcg aggaaactca ggagtacgaa gatcaggtaa ctggaatgat
     1141 gaagcctgta gccgttcagt aaggattaga ggagactgaa aatgaaatac gtaagacaat
     1201 tctggattat tttactgata tccgcaatgg gagaggcatt acatgttctg atcccgcttc
     1261 ctgtacctgc aagtgtgtac ggactggtga tcatgctgat cgcactggga acacacatta
     1321 tcaggctgga acaggtcaaa gaagcagctg aatttctcat agagatcatg ccggttatgt
     1381 ttattccggc aggtgtcgga ttactgacag cctggggcat actgaaaccg gtatgtgtgc
     1441 cgatcattct gatcacagtg atcaccacag ttgtggtaat gattgtgaca ggtcgtgtca
     1501 cacaggcagt tattcgtatg gacagaaaga aaggacagaa aagatcatga cacaatttat
     1561 gagtaattcc ctgttttttg gcgcagcaat cagcctgatc gcatatgagg caggacttct
     1621 tttgaaaaga aaatttaaga tggcaatttt taatccgctt ctgatcgcga tcattgcagt
     1681 aatagctgtg ctttgtttac tgcatattga ttatgacacc tataatcaga gcggtcagta
     1741 tatcagctac ctgctcacac ctgcaacggt ctgcctggca gttccgctgt atcagcagat
     1801 ggaacttctg aaaaagaatc tgaaagctgt aatcattgga attgtttccg gtgtgcttgc
     1861 cagtctggtc agtgtgctga tccttgcaaa gctgttcagc ctgagtcatg agcagtatgt
     1921 aacacttctt ccgaagtcta ttaccacagc aatcggaatg ggtgtttccg aagaactggg
     1981 tggtatagta acgattacag ttgcagttat tatcattacc ggtgttcttg gaaatatgct
     2041 cgcagagact gtgatcaaac ttgcgcgtat tgaagaacca atcgctaagg gcctggcact
     2101 tggaacctct gcccatgcta tcggtactgc aaaggcaatg gagctgggtg aaatagaagg
     2161 tgccatgagc agtcttgcga ttgcggttgc aggtctgctg acagttgtag gtgcatctgt
     2221 gtttgctcag tttatgtaaa aaatataaca ttaaaatcag ataagaggat tgtcgtggca
     2281 ggtgatgagt ggcacggcag tcttttttat gttataggaa attactccgt aatgttccta
     2341 taacataaaa aagccctccg ggcaggattc tttcttaatt ttaagtttca gtatccggaa
     2401 aaatttataa tgaaaaaaaa cggcaaacaa atgttctaaa aactcttcca tttttgaaat
     2461 gggtatgtta ttatatatca aaagaaagcg aatacatgtt cggaaaatat gttcgaacat
     2521 aatcgatcag aggataaggg cgatttttat ctgtctgaca tgaaggagga gtaaaatgat
     2581 gaatgcagta aataccacac agagaatgag aagaaaggaa agacataaat tttcaaggac
     2641 aatggcagtg atcctggggc tgatcgtcac cttggaagtg atggttgcag gtggtatgat
     2701 atttaatatt gattttcatt ctgctgacat ggttgcagta gttatgggat ttatggtgtt
     2761 atatgcgggg attgtggcaa tcctgacaga caattgatta ctgtacggaa aatgttactg
     2821 agaacggagt gaacagtaac accggaaaat tatgtgataa gatatttccg gcagaacatt
     2881 agacaaaaag cataccagat gatatgatta gcagtagatg atcagagaag agataacgtg
     2941 aaaataatgg gacagaaagt aagaaaggga aattatgact gtaaaatcaa gtcttttgga
     3001 aatgctggag aagaataaag gagaagtgct ttccggtgag agcattgccg gtgagttggg
     3061 atgtaccagg gcagcagtgt ggaaggcagt aaagtcgctc cgggaagaag ggtatcatat
     3121 agaagcagga ccgaataaag gatatatgct tgcgaaggat acgaaccgtc tgtcgcagga
     3181 aggaatccgc ctgcttctgg atgatccgaa ggttaagata gatatttatg atgaactgga
     3241 atctacaaac cagactgcca agaaagaagc tatgatggga gaggccggac atggggcatt
     3301 tgtcattgcc aggagccaga ctgcaggcag ggggcgcaga ggccgggaat tctattctcc
     3361 tgcagataca ggtctttaca tgagtgtgat cctgaagcca caaggaacca tacatgacag
     3421 tctgctgatc acaactgcgg cagcagtggc agtctacaga gcagttgctc agctctgtgg
     3481 aatccagctt gatattaaat gggttaatga tctgttttat aaaggaaaga aagtctgtgg
     3541 aatactcaca gaggctgtca cagattttga gagtggaaat attgagtttg tggtagttgg
     3601 catgggattg aacctctatc tggaccagga gaacctgcct ggggaattaa gaaatatagc
     3661 gggagctctt tatgagacaa aagaagatgc agaacagact gaccgtaata aactgactgc
     3721 aacgatcgtg aacgagctgc gaaaagaaac agcagatctg aaattatctc cggattatgt
     3781 gacacataac atgatcccgg gacatcagat cacaattaca gacggaaact cttccagaca
     3841 ggcatttgca ttggagatat gcagggacgg acggctgaaa gtacgggaag aggatggaca
     3901 ggagacaata ttatcctttg gagaagtttc ggtatccatg tgaggataat gagaatggga
     3961 ttgttacatg aaggtagatt ccttgatgtg acaatcccat tttgtgtcat gattcagaat
     4021 ttgttgtttg tgatgaagca tccttctgag acacttcggc ggcagaatct tttttcagag
     4081 ataccgggtt gccggtagtg gtgttatctg tgacatctgt atctgtgcag caatcgggat
     4141 tcgttgtatc tgtggtgttt ttagctgcgt cagttgttat atccgaaata ttctgctgat
     4201 acggacgata aggtgtccag taatgttctt cctcagtaaa gctgtcgggc tgttcttcat
     4261 ccagtgcatc cttcttgcgg gtgagaatat aaatccctgc aaaaatgata gcagctgcaa
     4321 tcacaagctg cggcagatga tatgtgaatc tgccgataac atcagccaga tatccgggta
     4381 agatcatata gaggaaatct accagggtgt tccacagaat agaagcacca aggaaaatca
     4441 gaagaatcgc agttatcttt ccgtgatgtt taagcagaga actgatatct cctgccagtt
     4501 catccatatg aaggacatag gaatcttcta tggagtaaaa ttcttcctca gaaagagact
     4561 tcagattgtg cacattaaag aaactgtaga accagatgat cgggatcagg aacacaagcc
     4621 agtccattcc ggtacatgct ccgatggcaa agacacccca gaataagaac ataatactga
     4681 ttccctgttt tttgaatccc atgtacattt ctcctgcacc tgggatcagg gagaagataa
     4741 aaagccagaa tccacgtttt tgtttttgca tacttttatt tgcctccatt cattaatttg
     4801 ccggaaaaat cactgatata ttttataaaa gaaccggtgc catcaccaat gttctgtccg
     4861 agttctctgg tgaactggta gagcctgccg ttttcatgct caggtaatat tctgtctgtc
     4921 tgagagaaag tatcctgatg taaggaagtg aaatccaggt tcgggatact gaataaaagc
     4981 agcagggcaa ccatcacacc ggcagcagtc tgaagactgt aatggagcag ccggattttg
     5041 cgggatgctt tatttgctgc ccgggcaagc tgcacttcac cggagtctgc tttgtccaga
     5101 atctgttcca tcagatagga gggagcagta tccgtactgc tgtcttcctg ttccatcatc
     5161 tgatccagac agaaatcgca gtgatcaaga tgtgacagaa actcaatgac ttcctgtgtg
     5221 ttcatatttc cgttgtcaaa ttctattaaa tccttcttat taatatgcat ctaatcaccc
     5281 ctttcaagca tttttttcag cattgccctt gcgcgataga gctgtgtctg aattgttttc
     5341 ggatttttcc ctgtctgctg tgcaatttct tcttctgtaa gtggatcttc tgtcccaatt
     5401 actccctgtg tttcaacatg aattttatgt cctgcttttt ctgccgcatt aattaatttt
     5461 tcttttg
//
LOCUS       DBMDXG010000098         5451 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3716_length_5451_cov_2.151776, whole genome shotgun sequence.
ACCESSION   DBMDXG010000098 DBMDXG010000000
VERSION     DBMDXG010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3716_length_5451_cov_2.151776"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="ACZPD3_04115"
     CDS             complement(<1..438)
                     /locus_tag="ACZPD3_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1415224.1"
                     /translation="MATKIDIISGFLGAGKTTLIKKLLKEAYADEQVVLIENEFGEIG
                     IDGGFLKEAGIQIREMNSGCICCSLVGDFGTSLKEVVDKYHPDRILIEPSGVGKLSDV
                     IKAVQGVQGDVDIVLNSYTTVVDAKKCKMYMRNFGEFFDNQVEY"
     gene            complement(775..1008)
                     /locus_tag="ACZPD3_04120"
     CDS             complement(775..1008)
                     /locus_tag="ACZPD3_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polya polymerase"
                     /protein_id="HIR1415225.1"
                     /translation="MKVSNIKDIDKFFSVVDSCEGRVELVTGEGDRLNLKSKLSQYVS
                     LANIFSGGEIPELEIVAYEKDDIDKLMSFMING"
     gene            complement(1222..3108)
                     /gene="glgB"
                     /locus_tag="ACZPD3_04125"
     CDS             complement(1222..3108)
                     /gene="glgB"
                     /locus_tag="ACZPD3_04125"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR1415226.1"
                     /translation="MGVSMKFTDLDQYLFGQGTNYEVYKKLGAHPTTYRRKKGVYFAV
                     WAPNAQSVSVIGEFNDWEEEANPMEKVGPIGVYEVFVPGAKIGQLYKFFIVGAHGEKL
                     YKADPYANEAELRPGTASRITDITDYKWKDATWIKNREKFDEQVEPMAIYEVHPGSWK
                     KHPQSEENEKGFYNYREFAHALAAYVKEMGYTHVELMGIAEHPFDGSWGYQVTGYYAP
                     TSRYGTPQDFKYMVDYLHQNKIGVILDWVPAHFPKDAHGLANFDGTAVYEHADPRQGE
                     HPDWGTKIYNYGRPEVKNFLIANALYWVEECHVDGLRVDAVASMLYLDYGKKDGEWIA
                     NKYGGNQNLEAIEFFKHLNTVVLGRNHGTVMIAEESTAWPMVTGDAEKDGLGFSLKWN
                     MGWMNDFLEYMKLDPYFRKFNHNKMTFSMSYAYSERYVLVLSHDEVVHLKCSMLEKMP
                     GELPDKFRNLKAAYSFMNCHPGKKLLFMGQEFGQLREWSEERELDWFLLNEEPHKELQ
                     NYVHDLLTIYKKYPALYAGDNNPEGFEWINANDGDRSIFSFVRKSPTKRNNILYIVNF
                     TPVDRPDYRVGVPKKKQYKLIMDENGLLEQPKTFKAVKQECDNREFSFAYPLPAYGVA
                     IFTY"
     gene            complement(3522..4874)
                     /locus_tag="ACZPD3_04130"
     CDS             complement(3522..4874)
                     /locus_tag="ACZPD3_04130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR1415227.1"
                     /translation="MDKRVLRERMRRKKRQMMIRHYTKIGLCVIGMILAVVFVVRGII
                     IPIAHHVAGGGSSDQTVQAQADTEEVQANSDAAVRQPLKGKSDTDKIAAMTAGWHEDA
                     NGKWYQNTDGTYFSNGFQDIDGVTYSFDENGYIQTGWVEKGVKDYYFNEDGSYDPSKV
                     RPMLALTFDDGPGEYTDELLDCLEQNNAHATFFMLGQNVSSYPDAPKRMLELGCEIGS
                     HSWDHTQLTTIDLDAVAKQFSDTDDALIQACGQAASVARAPYGDGNSDIYNTVNKPFF
                     MWSLDTEDWKLLDADADYSAVMNGDLTDGTIILMHDIHEPSVKAALRLIPDLIAQGYK
                     LVTVSEMAEAKNVTLQNACYVDFWPSTLSNGDVPGYQGGSDAAASADGTDGTSDASAD
                     SSDGSTDSSDGSEDYSDGSSDDGSYDDGSSDDGSYDDGSSDDSEDYSDDSVDYGDGTE
                     "
     gene            complement(4991..>5451)
                     /locus_tag="ACZPD3_04135"
     CDS             complement(4991..>5451)
                     /locus_tag="ACZPD3_04135"
                     /inference="COORDINATES: protein motif:HMM:NF017509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR1415228.1"
                     /translation="DSQGISQDDFDEQCQQYAEGKVKQNLIVQGIMDAEGLSLDDKES
                     LQLQDKLVEQMGVSSIAELVGTYGQDYVDESVGLLRVEEFIIKNASVSEKVANGDVLA
                     DDADAVAENAEQDSDQNVSDEDTDDSGQDNSDVDENLEEELGTEDVDQSE"
BASE COUNT         1479 a         1426 c          840 g         1706 t
ORIGIN      
        1 atattcaacc tgattatcaa agaactcacc aaagtttctc atatacattt tgcacttctt
       61 tgcatcaact acagttgtat agctgttcag aacgatatcc acatctccct gaacaccctg
      121 tactgccttg ataacatctg aaagtttacc aacacctgat ggctcgatca ggattctgtc
      181 cggatgatat ttgtccacaa cctctttcag agaagtgccg aaatctccca ctaatgaaca
      241 gcagatacat ccggaattca tttcccggat ctggattccc gcttccttca ggaaaccacc
      301 gtcaatgcca atctcaccaa attcgttctc gatcagtaca acctgctcat ctgcataagc
      361 ctctttcaga agtttcttaa tcaatgtagt tttaccggct ccaaggaaac cggaaataat
      421 atcaatctta gttgccatta tcttatttct cctcttcgtt aaaatttata tttctgaata
      481 actgccatat agccgtcatt ttttatattc tgccattttt atgaacaaac agaatagctt
      541 ttagaactgc catcattatc tgtctgttta tatatttgcc tgtaacgtac tcattataca
      601 taagatgtcc aatgtattca agtctgccat caacgcattt tgaccaggaa acacttgtgc
      661 cccctggttt acagatatcc tgcagttgtt caatactttc agaacaacct gcctgttcac
      721 atcaaagaaa ggggcatgat tcaccatcat gcccccgcct gtatacacta atgatcaacc
      781 gttgatcata aagctcatta atttatcaat atcgtctttc tcataagcaa cgatctcaag
      841 ttccggaatc tctccgcctg agaaaatgtt tgccagagat acatactggg ataatttaga
      901 tttaaggttc agtctgtcac cttcgcctgt tactaattct actctgcctt cgcagctgtc
      961 aactacagaa aaaaatttat cgatgtcttt aatgttagat actttcatgt tcattctcct
     1021 ttcaacatgg aaaccattat tctctctatt ccttatgttc gcgataatta taacgcagat
     1081 aaatttcaaa agcaattatt tatatttcac gaatttttca tatattatcg ggcttaccct
     1141 gcaatattgt caaataaata acatgcccct gacaataatt ctctcagata ttcacatata
     1201 ccctgaaatc atccctcaaa attaatatgt aaaaatcgca accccataag ccggcaatgg
     1261 atatgcgaag gaaaactctc tgttatcaca ttcctgtttc actgccttaa atgtctttgg
     1321 ctgctccaga agtccattct cgtccatgat cagcttgtac tgttttttct tcggaacacc
     1381 aactctgtaa tccggacggt caacaggtgt aaagttaact atgtaaagaa tattgtttct
     1441 ctttgtagga gactttctca caaagctgaa aatacttctg tccccgtcat tcgcattgat
     1501 ccactcaaat ccttccggat tattatcacc ggcataaagt gccggatatt tcttataaat
     1561 agtcaggaga tcatgcacat aattctgaag ttccttgtgt ggctcttcat tcagaaggaa
     1621 ccagtcaagt tctctctcct cactccattc acgaagctgt ccgaattcct gtcccatgaa
     1681 cagaagcttc ttacccggat ggcagttcat aaatgaatag gcagccttta aattcctgaa
     1741 tttgtccggt aactctcccg gcatcttctc aagcatggag catttcagat gaaccacctc
     1801 atcatgagaa agaactaata catatctctc actgtatgca tatgacatgg agaaagtcat
     1861 cttattatga ttaaatttac ggaaatacgg atccagcttc atatattcca gaaagtcatt
     1921 catccatccc atattccact tcagactaaa accaagtccg tccttctctg catcccctgt
     1981 aaccatcggc catgcagtag attcctcagc gatcatcaca gttccatgat tccttccaag
     2041 aactactgta ttcagatgct taaagaattc aatagcctcc agattctgat ttccgccata
     2101 tttatttgcg atccattctc catccttctt accataatca agataaagca tggaagcaac
     2161 agcatccaca cgaagtccgt ctacgtggca ctcctccacc cagtaaagag catttgcgat
     2221 caggaagttc ttaacctccg ggcgtccgta attataaatc ttggtacccc agtccggatg
     2281 ttctccctgt ctcggatctg catgctcata cactgcggtt ccgtcaaagt tcgcaagacc
     2341 atgggcatct ttcggaaaat gtgcaggtac ccagtcaagg ataactccaa tcttattctg
     2401 atgtaaataa tccaccatat acttgaaatc ctgcggcgtt ccatatctgg aagtcggtgc
     2461 ataatatccg gtcacctgat atccccagga accgtcaaaa ggatgctctg cgattcccat
     2521 aagctctaca tgggtatatc ccatttcctt cacataggct gccagggcat gtgcgaattc
     2581 tctatagtta taaaatcctt tttcgttttc ctctgactgc ggatgcttct tccatgaacc
     2641 cggatgaacc tcatagattg ccataggctc tacctgctca tcaaacttct ctctgttctt
     2701 gatccatgtg gcatccttcc atttatagtc tgtgatatct gtgatcctgg aggctgtacc
     2761 cggacggagt tcagcttcat ttgcataggg atctgcctta taaagcttct ctccatgcgc
     2821 acccacgata aagaatttat acagctgtcc gatctttgcc cccggaacaa atacctcata
     2881 aacaccgatc ggaccaacct tctccatcgg gtttgcttct tcctcccagt cattaaattc
     2941 accaattaca gaaacagact gtgcattcgg tgcccagacc gcaaaatata cacccttttt
     3001 tcgtctgtat gtagtcggat gggcacccag tttcttatat acttcataat tggttccctg
     3061 tccaaataaa tattgatcca aatccgtaaa tttcatactc acacccatct ggagtcctcc
     3121 cttaacttcg gtttctatct tttttgcaga tactgattcc ataaaataat actgtaacag
     3181 gatatcttac gttactgttc actccattct cagtaacgta gccaaaattc attccagatt
     3241 gcctgcggca atggaatttt ggcatatgaa cagtaactat cttacagcaa gttcaatcag
     3301 gaatacgaac tgacttttag tcattctata agccttatta taccaacgga cattccatac
     3361 gtcaattaaa aatatgtgta tatttaacaa tttttttata tattttatgt gttttttgta
     3421 aaaaacataa agagcaaaga agtacctttg ctctcttctt tgctctttcg tatgttcgta
     3481 attactctgt atgatccaca ctgcaggatt gcatatgttt tttattccgt tccatctccg
     3541 taatctacag aatcatcact gtaatcttcc gaatcatctg aacttccatc atcataactt
     3601 ccgtcatccg aacttccatc atcatagctt ccatcgtctg aacttccatc cgaataatcc
     3661 tcacttccgt ctgaggaatc tgtgcttccg tcagaagaat ctgcgcttgc atcagatgta
     3721 ccatctgttc cgtctgcaga agctgctgca tcggaaccgc cctgatatcc cggcacatct
     3781 ccattactga gtgtactcgg ccagaaatcc acataacatg cattctgtaa ggtcacattc
     3841 ttggcttctg ccatctcact taccgttaca agtttatatc cctgtgcaat aagatccggt
     3901 atcaatctca aagcagcctt tacagatggc tcatgaatat catgcatcag gatgatagtt
     3961 ccatctgtca gatctccatt cattactgca ctgtaatccg catctgcatc caaaagtttc
     4021 cagtcttctg tgtccagtga ccacataaag aacggcttat ttacagtatt ataaatatct
     4081 gaatttccgt ccccgtaagg cgcacgcgcc acactcgcag cctggccaca tgcctgaatc
     4141 aatgcatcat ctgtatcaga gaactgcttt gcaactgcgt caagatcaat tgtcgtcaac
     4201 tgtgtatgat cccatgaatg acttccaatc tcacaaccaa gttccagcat acgttttggc
     4261 gcatccggat atgaactgac attctgacca agcataaaga atgttgcatg agcattattc
     4321 tgttccagac aatccagcag ttcatctgta tactctcccg gaccatcatc aaaagtcagt
     4381 gccagcatcg gacgtacctt actcggatca taagatccgt cttcattaaa gtaataatcc
     4441 ttcactccct tttctaccca tcctgtctgt atatatccgt tttcgtcaaa agaataggta
     4501 acaccatcta tatcctgaaa tccatttgaa aaatatgtac catctgtatt ctgataccac
     4561 tttccgttag catcctcatg ccatccggct gtcatcgcgg caatcttatc tgtatctgat
     4621 ttacccttca atggctgtct cacagcagca tcggaatttg cctgaacttc ctctgtatct
     4681 gcctgagcct gtacagtctg atcactgctt ccgcctccgg ctacatgatg tgcaatcgga
     4741 ataataattc ctctcactac aaacaccacc gccagaatca tgccaataac acaaagtccg
     4801 atctttgtat aatgcctgat catcatctgg cgtttttttc tgcgcattct ttcgcgcaac
     4861 accctcttat ccatggtttc ctttccccgc ttctttttgt taattagtcc ctgacagcgt
     4921 acatcacagt acggagtaat ccacttttat caataatcat aactcattta tcaggtaaat
     4981 acaatgccaa ttattcactc tgatcaacat cttccgtacc taattcctct tccagattct
     5041 catctacatc tgaattgtcc tgtccggaat catcagtatc ttcatctgat acattctgat
     5101 cactgtcctg ttctgcattt tctgccacag catctgcatc atctgccagc acatcaccgt
     5161 ttgcgacttt ctcactcaca ctcgcatttt ttataatgaa ctcctccact ctgagaagtc
     5221 cgactgattc atctacataa tcctgtccgt aagttccaac cagctctgca atactggaaa
     5281 cacccatctg ttctaccagt ttatcctgca gctgaaggct ctctttgtcg tcaagtgaca
     5341 gtccctctgc atccatgatc ccctgcacga tcaggttctg cttaacctta ccctctgcat
     5401 actgctgaca ttgttcatca aaatcatcct gcgaaatacc ttgactgtct g
//
LOCUS       DBMDXG010000099         5434 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3732_length_5434_cov_2.161635, whole genome shotgun sequence.
ACCESSION   DBMDXG010000099 DBMDXG010000000
VERSION     DBMDXG010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_5434_cov_2.161635"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="ACZPD3_04140"
     CDS             complement(<1..879)
                     /locus_tag="ACZPD3_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1415229.1"
                     /translation="MVKIDLITGFLGSGKTTFIRKYAQYLMDAGNNIGILENDYGAVN
                     VDMMLLQDLMGENCELEMISGGCDKDCHRRRFKTKLIAMGMCGYDRVIVEPSGIFDVD
                     EFFDILHEEPLNRWYQIGNVIAIVDSKLERDLSEEADFILASEVADAGCIVMSKSQDA
                     SPEEIQGTIEHVNQALEKVHCSRRFHCEMNGVDTANVIHKNWDEMSKEDFDRIASCGY
                     VMASYRKPEFEAEGAFTSLYFMNVKMTEKELREAAEKILSDPECGRVFRMKGFMRVDS
                     DSEDGSGLSAWAECDRR"
     gene            complement(1059..2942)
                     /locus_tag="ACZPD3_04145"
     CDS             complement(1059..2942)
                     /locus_tag="ACZPD3_04145"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415230.1"
                     /translation="MNKKKKLDQNTIQTAKRLLKYMTGTHKIQFIIVFICIFISSAAS
                     IAVSLSLKFLLDDFIIPLIGQTDPNFAELYKALAVLGCIFALGVIATFIYTRMMVYIG
                     QGVLKSVRDDMFEHMQTLPIRYFDQNTNGSVMSLYTNDTDTLRQMISQAIPQALMALF
                     TIVVTFISMLLLSPLLTILAVVIIFIMLKVTSKIGSNSGKYFIRQQVSLADVTGFVEE
                     RMNGQRVVKVFNHEDKSKEEFDKLNEALFESSANANKYGNMMGPVIGNIGNLQFVLTA
                     VLGGLLSVTGVGGITLGVMASYLQFTKSFTQPFMQVAQQFNAIVMALAGAERIFRLID
                     EKPEEDEGYVELVNARKDANGNITECKERTGMWAWKHPHSADGSVTYTELTGDVRFED
                     VTFGYNPDKVILKDISLFAKPGQKLAFVGSTGAGKTTITNLINRFYDIQEGKIRYDGI
                     NITKIKKDDLRRSLGIVLQDTHLFTGTIKENIRYGKLDATDEEVYEAARLAHADQFIK
                     MLPKGYDTMLSGDGEELSQGQRQLLSIARAAVANPPVLILDEATSSIDTRTESIVQKG
                     MDNLMKGRTVFVIAHRLSTIRNSDAIIVLDHGKIIERGDHEDLIKLKGTYYQLYTGKL
                     ELS"
     gene            complement(2935..4668)
                     /locus_tag="ACZPD3_04150"
     CDS             complement(2935..4668)
                     /locus_tag="ACZPD3_04150"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415231.1"
                     /translation="MNKKLLRSVREYKKQSVLAPILVILEVLMEVLIPLEMAKIIDVG
                     IANGDMSYIIQRGVILVAMAMLSLFFGVQAGNMAAVAAAGYAKNLRHDIFYKVQDFSF
                     KNIDHFSTSGLVTRLTTDITNVQQAYMMSIRLLARAPFMIILSWIMTLTINKPIAVLF
                     LIVVPVLGGTLIFIAKKAHPHFIKVFDEYDNLNNSVQENVNASRVVKAFVREDYEIEK
                     FHGISRYVYNLFTKAEKIVAWNSPVMMFVMYTVIIIIVAIGGRGIVLGGMETGELTSI
                     IVYALQILMSLNMVTFVFVMIMIAEASTDRIVEVLDEVPEMQDKADAVKNVADGSIDF
                     NHVDFSYAGEGGNLSLKDVNLHIKSGQTVGIIGGTGSAKSTLVQLIPRLYDVTKGNVQ
                     VGGVDVRNYNLEVLRDQVSMVLQKNVLFSGSIYDNIRWGDEHASEEEVRRVCKLAQAD
                     GFVSEFPDGYDTMIVQGGNNVSGGQKQRLCIARALLKKPKILILDDSTSAVDTRTDAL
                     IRKAFREEIPNTTKIIIAQRVSSIEDADQIIVLDDGKIAGVGTSEELLKTNDIYREVY
                     ESQVKGGGDDE"
     gene            complement(4705..5172)
                     /locus_tag="ACZPD3_04155"
     CDS             complement(4705..5172)
                     /locus_tag="ACZPD3_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR1415232.1"
                     /translation="MKQKLNEMYVGRLIHILSHTMKRHNPAEVMENDDLTTMQKHVLK
                     FILLETMHRDLYQKDIEEEFQIRKPTVTGILKLMEKNGYIYRESAKQDARLKQIIPTE
                     KAEKIRPAILKSIEEGEAKMLRGIPKEDVELCKQVLWQMYENRKKSCDSRNDF"
BASE COUNT         1413 a         1382 c         1013 g         1626 t
ORIGIN      
        1 ccgtctatcg cactctgccc atgcagatag tccagaaccg tcttcgctgt cactgtctac
       61 tctcataaat cccttcattc ggaacacacg cccacattca ggatcagaca gaatcttctc
      121 agctgcttcc cgcagctcct tctcagtcat cttcacattc ataaagtaca gagaagtaaa
      181 ggcaccctct gcctcaaact ccggtttcct gtagcttgcc atcacatatc cacaggatgc
      241 aatccggtca aagtcctctt tcgacatctc atcccaattc ttgtggataa cattagcagt
      301 atccacacca ttcatttcac aatgaaatct cctgctgcaa tgaaccttct ccaatgcctg
      361 attcacatgc tcaatcgtac cctggatttc ctcgggtgat gcgtcctggc tcttgctcat
      421 cacaatgcat ccggcatctg ccacctcaga agccagaatg aaatccgcct cctctgacag
      481 atcacgctcc agcttagaat ccacaattgc aatcacattc ccaatctgat accaacgatt
      541 taacggctcc tcatgaagaa tatcaaagaa ctcatccaca tcaaaaatcc cagacggctc
      601 cacgatcaca cggtcataac cgcacatacc catagcaatc agtttcgtct taaatctcct
      661 cctgtggcag tccttatcac accctcctga gatcatctcc agctcacagt tctctcccat
      721 cagatcctga agcaacatca tatccacatt cacagccccg taatcattct ccagaattcc
      781 aatattatta ccagcatcca tcagatactg cgcatacttc ctgataaacg tcgtcttccc
      841 tgaaccaaga aaaccagtga tcagatctat ctttaccatc gtatatctct cccttgctgc
      901 tgcataattt ttctttttta tccgtaaacc ggaacttcat catctgtgat ccacatacct
      961 gtacgatcat agcattataa cgcagaaaac gcaacgcccg caaagaaaga ccacaattat
     1021 tcatgttctg aaataattgc agtctttctg atagttcctt atgacaattc cagcttacca
     1081 gtataaagct gataataagt tccttttaac ttaatgaggt cttcatggtc gcctctttca
     1141 atgatctttc catgatccag tacgatgatg gcatcgctgt ttctgatggt tgaaagtcga
     1201 tgggcgatca caaatacagt acggcctttc atcaggttat ccataccttt ttgtacaata
     1261 ctctctgtac gggtatcaat acttgaggtt gcttcatcca ggatcagtac aggcggattg
     1321 gctactgctg ctcttgcaat ggagagaagc tggcgctgtc cctgggacag ttcctcaccg
     1381 tctccgctta acatggtatc atatcctttt ggaagcattt tgataaactg gtctgcatga
     1441 gccagtcttg ctgcctcgta tacttcctca tctgtagcat ccagtttacc gtagcggatg
     1501 ttttctttaa tagttcctgt aaacaggtga gtatcctgaa gtacgattcc cagagaacgt
     1561 ctcaggtcat ccttcttaat cttggtaatg ttgattccat cgtaacggat tttaccttcc
     1621 tgaatatcat agaaacggtt gatcagattt gtgatcgttg tctttcctgc acctgtggaa
     1681 cctacgaaag caagtttctg tcccggcttg gcgaagaggc tgatgtcttt cagaataact
     1741 ttgtccgggt tatatccgaa tgtaacatct tcgaatcgga catctcctgt cagttctgta
     1801 taagtaacgg aaccatctgc agaatgtgga tgtttccatg cccacatgcc tgttctctct
     1861 ttgcattctg tgatattgcc gttggcatcc tttcttgcat taacaagttc tacgtaacct
     1921 tcatcctctt ccggcttctc gtcaatgagc ctgaagatac gttctgcacc tgcaagtgcc
     1981 ataacaatgg cattaaactg ctgtgccacc tgcataaatg gctgggtaaa gctcttggtg
     2041 aactgtaaat aggaagccat aacaccgagg gtgatgccgc ccacacctgt tacagagaga
     2101 agtcccccaa gtactgctgt cagtacgaac tgcaggttac cgatgttacc gatcacaggt
     2161 cccatcatgt ttccgtattt attggcgttt gcagaactct cgaagagtgc ttcgttgagt
     2221 ttgtcgaatt cttcttttga tttatcttcg tggttgaaaa cttttaccac acgctgacca
     2281 ttcattcgtt cttccacgaa accggtcaca tctgccagag atacctgctg acggatgaaa
     2341 tatttgccac tgttggaacc aatcttactg gtaaccttca gcataataaa aataatcaca
     2401 actgccagaa ttgtaagcag cggacttaac aataacatgg aaataaaggt aaccacgatc
     2461 gtaaacagcg ccatcagcgc ctgcgggatc gcctggctga tcatctgtcg cagggtatct
     2521 gtatcgttgg tatagaggct catgacagat ccgtttgtat tctggtcgaa atagcggatc
     2581 ggaagagtct gcatatgctc gaacatatca tcacggacac ttttaagcac accctgaccg
     2641 atataaacca tcattcgggt ataaataaat gtggcaatca caccaagtgc aaaaatacag
     2701 ccaaggactg caagtgcctt atagagttct gcaaagttcg gatctgtctg tccgatcagc
     2761 gggatgataa agtcatccag cagaaatttc agggacaggg atactgcaat acttgctgcg
     2821 gaactgataa agatgcagat aaatacgatg ataaactgta tcttatgggt tcctgtcata
     2881 tatttcagaa gtcttttggc agtttggatg gtgttctgat ctaatttttt cttcttattc
     2941 atcgtctccg cctcctttga cctgtgattc atatacttct ctgtaaatat catttgtctt
     3001 cagcagttct tctgaagtac ccacacctgc gatcttaccg tcatccagta cgatgatctg
     3061 atctgcatct tcaatagaag aaactcgctg tgcgataata atctttgttg tgttcgggat
     3121 ctcctcgcgg aaagccttac gtatcagggc atctgttctt gtatctactg cactggtaga
     3181 gtcatccagg atcaggatct tcggtttctt gagaagagcc cttgcaatac acagacgctg
     3241 cttctgaccg ccggatacgt tgttaccgcc ctgaacgatc attgtatcgt agccatccgg
     3301 gaactcactg acgaatccat ctgcctgggc cagcttacag accctccgaa cttcttcctc
     3361 actggcatgt tcatcacccc agcgaatatt gtcatagatg cttcctgaga acagcacatt
     3421 tttttgaagt accatggaaa cctggtcacg aagtacttcc agattgtagt tgcgtacatc
     3481 cacgcctccc acctggacat taccctttgt gacatcatag agtctgggga tcagctgcac
     3541 cagggtagac tttgcacttc cggttccgcc gatgatacct actgtctgac cggatttaat
     3601 atgaagattt acatccttca gggaaagatt tcctccctct cctgcatagc tgaaatctac
     3661 atgattaaag tcaatggaac catctgccac attctttact gcatcagctt tatcctgcat
     3721 ctcaggaact tcatccagaa cctctacaat acggtctgtg gatgcttctg ctatcatgat
     3781 cataacaaac acgaaagtta ccatgttcag ggacatgaga atctgaagtg cgtagacaat
     3841 gatacttgtc agctcaccgg tttccatgcc accgagaaca atacctcttc cgccaattgc
     3901 aacaatgata atgattacag tatacatgac aaacatcata accggtgagt tccatgcaac
     3961 gattttctct gctttagtaa acagattata aacatatctg gaaattccat ggaatttctc
     4021 aatctcatag tcctctctta cgaaagcctt aactacacgt gaggcattta cattctcctg
     4081 aacggaattg ttcaggttgt catattcatc aaagaccttg atgaaatgcg gatgtgcctt
     4141 tttcgcaata aagattagtg taccgccaag gacaggaaca actatcagga aaagcactgc
     4201 gatcggtttg ttgatcgtca gtgtcataat ccatgacagg atgatcataa acggtgccct
     4261 ggcaagaagg cgaatactca tcatgtatgc ctgctgtaca tttgtgatat cagtggtcag
     4321 tcgtgtgacc agacctgatg ttgaaaagtg gtcgatattt ttaaaggaaa aatcctgcac
     4381 cttataaaaa atatcgtgtc gtaaattctt cgcatatcct gctgctgcaa ctgccgccat
     4441 atttccggcc tgtacgccga agaacaggga aagcattgcc attgcaacaa ggatgactcc
     4501 acgctggatg atatagctca tatctccgtt tgcaatacct acgtcgataa tcttcgccat
     4561 ctccagagga atcagaactt ccatcagtac ttccaggatt acaaggatcg gagccagtac
     4621 agactgcttc ttgtattccc ggactgaacg taaaagtttc ttattcatct tcgtcaatgt
     4681 cctccttatg tctcgttatt tctgttaaaa atcattcctg ctatcgcagg attttttccg
     4741 gttttcatac atctgccaga gaacctgttt gcacagttcc acatcctcct tcggaattcc
     4801 ccgtagcatt ttggcttcgc cttcttcaat actctttaag attgcaggac ggatcttctc
     4861 tgctttctct gtgggaatga tctgtttcag gcgtgcatcc tgtttcgcac tctctctgta
     4921 aatgtaaccg ttcttctcca tcagcttcag gatccctgtc actgtaggtt tcctgatctg
     4981 gaattcctct tcaatatctt tctgatagag atctctgtgc atggtttcca gaaggataaa
     5041 tttcagcaca tgcttctgca tggtcgtcag atcgtcattc tccatcacct ctgccggatt
     5101 atgccgtttc attgtatggg ataaaatatg tatcagcctg cctacataca tctcattcag
     5161 cttctgtttc atttcctcgt ctatctcgcc gcacctccta aaatcagtac ccatactatt
     5221 gcaaagccat ataatagtta ggttgctatc cattagttgc ctaactatta tatggcttta
     5281 gtttttttat gtcaatactc gtttttattt ctccttttga actactcaaa aattgttact
     5341 gttcactccg ttctcagtaa cacgcttcgc gatgcaggaa atatgaacct ccttgtgcaa
     5401 tgcccaagga gtatcgtttg cttcgcaaac ttaa
//
LOCUS       DBMDXG010000100         5430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3735_length_5430_cov_1.612685, whole genome shotgun sequence.
ACCESSION   DBMDXG010000100 DBMDXG010000000
VERSION     DBMDXG010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_5430_cov_1.612685"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(84..692)
                     /locus_tag="ACZPD3_04160"
                     /pseudo
     CDS             complement(84..692)
                     /locus_tag="ACZPD3_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
     assembly_gap    758..767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<768..1856)
                     /locus_tag="ACZPD3_04165"
     CDS             complement(<768..1856)
                     /locus_tag="ACZPD3_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1415233.1"
                     /translation="MLAHLHVKNLALIEEIEVEFGPGLNILTGETGAGKSILLGSMQL
                     ILGAKTSKNMIRENASYALVELLFQVENEKALETLKALDICPEDGQVLLSRKIMDGRS
                     INKINGETSTVGQMKAAAACLLDIHGQHEHQSLLYQDKQLAILDAYGKEKILPAKEKV
                     SLAYKEYSKCKTELGSMNMNEEQRNRELAFLEFEIKEIEKANLQPGEDEELEARYRKM
                     SNAKLIVDSLQLVHNLTGYESKEGAGETVGEALKEFSHVTQYDPELTPLAETLTSVDG
                     LLNDFNRELSAYLDELTFDEGEFYETERRLDLINGLKAKYGRTIEEIFTYRKLQEEKL
                     EKLHKYEENLQELKEHLRELENILEKKSD"
     gene            complement(1940..2398)
                     /gene="argR"
                     /locus_tag="ACZPD3_04170"
     CDS             complement(1940..2398)
                     /gene="argR"
                     /locus_tag="ACZPD3_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR1415234.1"
                     /translation="MKVARHEKIIELIHQYDIDTQEELASRLNEAGFKVTQATVSRDI
                     RALKLTKVAGKDGKSRYAIINNESGSLGEKYTRVLEDTLLSIDVGQNIIVIKTVSGMA
                     MGVAAALDALKWPEILGCIAGDDTIMCVAKTADMALGAAEKLRSFVRLNK"
     gene            complement(2417..3277)
                     /locus_tag="ACZPD3_04175"
     CDS             complement(2417..3277)
                     /locus_tag="ACZPD3_04175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR1415235.1"
                     /translation="MDRFYIITNSDKDKKLEITEKIADYLKTHHKNCEVQQAERKHEG
                     SFHYTDPDKVPDDTQCIIVLGGDGTLLQAARDVVHKEIPLLGINLGNLGFLAEVNQTS
                     LYSALDQLMADDYEVEERMMLEGRVYRGRKLIGQDIALNDIVIGRDGHLRVVRFKNYV
                     NDVYLNSYNADGIIISTPTGSTGYSLSAGGPIVSPNAAMTIMTPIAPHTLNTRSIIFP
                     AQDVITVEIGKGRHCDCEKGIASFDGDTFIPMVTGDCIQIRRADVKTKILKLNHLSFV
                     EVLRRKMRDS"
     gene            complement(3301..4110)
                     /locus_tag="ACZPD3_04180"
     CDS             complement(3301..4110)
                     /locus_tag="ACZPD3_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR1415236.1"
                     /translation="MKKRLDMLMMERALAPSREKAKAFIMAGDVYVDGQKEDKAGTMF
                     PETVKIEVRGNTLPYVSRGGLKLEKAMKNFDVTLDSKVCMDVGASTGGFTDCMLQNGA
                     VKVYSIDVGYGQLDWKLRNDPRVVCMEKTNIRYVVPEDLGEPADFSSIDVSFISLTKV
                     LLPVRNLLTDEGEIVCLIKPQFEAGREKVGKKGVVRDPAVHQEVIEKVRDYAMSIFME
                     PCHLSFSPIKGPEGNIEYLLHLKKHPEGTRVTDSLKVSVEAVVAEAHGQLD"
     gene            complement(4107..>5430)
                     /gene="dxs"
                     /locus_tag="ACZPD3_04185"
     CDS             complement(4107..>5430)
                     /gene="dxs"
                     /locus_tag="ACZPD3_04185"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR1415237.1"
                     /translation="ACPLICALRTAEAYTGMKMGVTKALKKVPKVGTALVDTMRKTKS
                     SVKQLFIPGMLFENMGLTYLGPVDGHNMRQMMKLFNEAKRVEGPVVVHVLTKKGRGYE
                     PASAHPDRFHGTGPFDIKTGRVLQKKTVPGYTDVFSKALVSLGEKNKKLTAITAAMPD
                     GTGLVEFSRRFPDRFFDVGIAEEHAVSFAAGLALGGLVPVVAIYSSFLQRAVDQILHD
                     VCMQKLHVIFAVDRAGLVGADGETHQGCFDLSYLSMMPNMTVLAPKNDRELEEMLAFA
                     VSFDGPIAIRYPRGSAHQGLQEYQAPVEYGRSEIIRKGKKIAVLGVGSMIPSCMEICK
                     GLKDDGYDPTFVNVRFVKPLDVDLLDELAKDHSLFVTVEENVKSGGYGEHVSAYMEAC
                     HPEIRVLSAAVWDRFVPQGNVESLRSRIGLGVEDIRQAIEDSEELREQ"
BASE COUNT         1364 a         1407 c          954 g         1695 t
ORIGIN      
        1 tcacagcaaa tccgaaaggt acaaaaataa gagagaacaa tccgtcacaa agattatcct
       61 ctcttagtat gtggtcaatt attttattta aatcttgtat ttttttgttt ctgtgccaat
      121 tctttcatct ctttcgcatt ctcaagaact gcaggtgtaa tttctgcacc acctaacagt
      181 cttgccagtt cgcggatgga atcatgttct ttaagcacat gaatctgggt aatagtctca
      241 gttccctgca gatgtttttc aatttcaaaa tgactgtctg ccatagctgc aatctgcggc
      301 agatgcgtga tgcagagtac ctgatgatgc tgtccgatca ctgccatctt ttcagagact
      361 ttctgtgcag ttcttccact gataccagta tcgatctcat cgaagatcaa tgtctcaatc
      421 tcatctctgt ctgcaaggat tgcctttaat gcaagcatga ttcgagacaa ctcaccgccg
      481 gatacgatct gtcccagtgg cttcagagtt tctccaggat ttgtagaaat gagatactgg
      541 atatcatcgg ttccattatc tgtgtagttt ttctttttcc ggaaatcaat ggcaaaattc
      601 acatccagga agtttaaatc cttcaatccc tgaataatct tctgctcaag ctgtttactg
      661 tattctttac ggatttctgc taattcatcc gactgtccac gttattttat atatccatac
      721 taccataatt ttgcacaatt atacaaatta caaataannn nnnnnnnatc cgactttttc
      781 tcaagaatat tttccagttc tctgagatgc tcctttaatt cctgtaaatt ttcttcatat
      841 ttatgtaatt tttccagttt ttcttcctga agtttgcgat atgtgaaaat ttcctcaatg
      901 gtacgtccat attttgcctt cagtccattg atcagatcca gtcttctctc tgtctcataa
      961 aactctcctt catcaaaggt cagctcgtcc agataagcgg ataattctct gttaaaatca
     1021 ttcagaagac cgtctacaga agtaagtgtc tctgcaagag gtgtaagctc cggatcatac
     1081 tgtgttacat gagagaattc ctttaatgcc tctccgacag tctctccggc gccttccttg
     1141 ctttcatagc ctgtgagatt atgtacaagc tgcagggagt caacgatgag ttttgcattg
     1201 ctcatttttc tgtaccgggc ttccagttcc tcatcctctc ccggctgcag atttgctttc
     1261 tcgatttctt tgatctcaaa ctccagaaaa gccagctcac gatttctctg ctcctcattc
     1321 atattcatgg aaccaagttc tgttttgcat ttgctgtatt ctttatatgc cagagatact
     1381 ttctcctttg caggaaggat tttttctttt ccatatgcat ccaggattgc aagctgctta
     1441 tcctgataca gaagtgactg atgctcgtgc tgtccatgga tatccaaaag gcatgcagca
     1501 gcggccttca tctgacctac agtacttgtc tctccattaa tcttgttgat gcttcttcca
     1561 tccataattt ttctggacag aagtacctgt ccatcttccg gacagatatc cagtgctttc
     1621 agtgtctcca gcgctttctc gttttctacc tgaaaaagca gctccacaag tgcataggat
     1681 gcattttccc ttatcatatt tttagaagtt tttgcaccca ggatcagctg catagacccc
     1741 agaaggatag atttacctgc tccggtctca ccggtcagga tattcagtcc gggaccaaat
     1801 tctacttcta tttcttctat cagtgcaaga tttttcacat gcagatgagc taacatttat
     1861 tgcctccata atcaagtcat ttgtaatgtt aaaagttcag gatcgtaact gcaaaggtat
     1921 tgagcagtta ctcgtgggat cacttattca atctgacaaa acttctcagt ttctctgctg
     1981 ctcccagtgc catgtctgca gtctttgcaa cacacataat agtgtcatca cctgcaatac
     2041 atcccagtat ttccggccat ttcaacgcat ccagggctgc tgccacgccc atggccattc
     2101 cggaaacggt tttgatcaca atgatattct gtcccacatc aatagaaagc agggtatctt
     2161 ccagtactct tgtatatttt tctccaagac ttcctgattc attattaata attgcataac
     2221 gggactttcc gtcctttcct gccacttttg tcagcttgag agcacgaata tctctggata
     2281 cagtcgcctg tgtcacctta aatcctgcct cattaagtct ggaggccagc tcttcctgtg
     2341 tatcaatatc atactgatga atcagttcaa taattttctc atgtctcgca actttcacct
     2401 tatttcctcc tgctgatcag ctgtctctca tcttccggcg caatacctca acaaaactta
     2461 aatggttcag cttcagaatc tttgtcttta catctgcccg ccggatctga atgcagtcac
     2521 cggtgaccat ggggataaat gtatctccgt caaaagatgc gattcccttt tcacagtcac
     2581 agtgtcttcc ttttcctatc tctacagtga tcacatcctg cgcaggaaaa ataatgcttc
     2641 ttgtattcag cgtgtgtggt gctatgggag tcatgatcgt catggcggca ttgggcgata
     2701 caataggtcc tcccgctgac agactgtatc cggtagatcc ggtaggtgtt gaaataataa
     2761 ttccatcagc attataagag ttaagataaa catcatttac ataattctta aaccggacca
     2821 cgcggagatg accgtctctg ccgatcacaa tgtcattcaa tgcaatatcc tgtccgatca
     2881 gttttctgcc gcggtacacc cttccttcca gcatcatgcg ttcttctacc tcgtaatcat
     2941 ccgccattag ctgatccaga gcactgtaca gagaggtctg attcacttct gcaagaaaac
     3001 ccagatttcc cagattgatt ccaagaagag gaatttcctt atgcaccaca tcccttgctg
     3061 cctgaagaag tgtaccatct cctcccagaa caataataca ctgtgtatcg tctggaacct
     3121 tatcaggatc tgtataatga aaggaaccct catgtttcct ttctgcctgc tgtacttcac
     3181 agtttttgtg atgtgttttc agataatctg cgattttttc tgtgatttca agttttttat
     3241 ccttgtcact gtttgtaata atataaaacc tgtccatagt ttcttcctga ctcagccttt
     3301 ttaatccaac tgtccatgag cctctgccac aacagcttct acagatacct tcaggctgtc
     3361 ggtaaccctg gtaccttccg gatgtttttt cagatgaaga agatattcga tatttccttc
     3421 cggtccttta attggcgaaa aagaaagatg acatggctcc ataaaaatac tcatggcata
     3481 atcgcgcact ttttcgatca cttcctgatg aacagcagga tctcttacaa cccccttctt
     3541 tccgactttc tctctccccg cctcaaactg cggcttgatc agacatacga tctcaccctc
     3601 atctgtaagg agatttctta caggaagcag tacttttgta agagaaataa aggacacatc
     3661 aattgatgaa aaatctgccg gttctcccaa atcctccggt accacatagc ggatattggt
     3721 tttctccata cagactacac gcggatcgtt acgaagcttc cagtcaagct gtccatatcc
     3781 tacatcaatg gaataaactt ttactgcccc gttctgtaac atacaatctg taaaccctcc
     3841 ggtggacgca cctacatcca tacagacctt actatccaat gtgacatcaa agtttttcat
     3901 agctttttca agtttcagcc caccgcggct tacatacgga agagtgtttc ctctgacctc
     3961 aatctttaca gtctccggga acatggttcc cgccttatct tctttctgac catcaacata
     4021 tacatcgcct gccatgatga atgcctttgc tttctcgcgg gatggcgcaa gggcgcgttc
     4081 catcataagc atatccaaac gtttcttcat tgctctctta attcctcact atcttctatt
     4141 gcctgtctta tgtcttccac tccaagtcca atccggctac gcaggctttc tacatttccc
     4201 tggggtacga aacgatccca gacagctgcc gacaacactc ttatttccgg atgacaggct
     4261 tccatataag cagacacatg ctctccgtat ccgccgcttt tcacattctc ttctactgtc
     4321 acaaacagac tgtgatcctt tgccagttca tcaaggagat ccacatccag tggtttcaca
     4381 aatctgacat ttacaaaagt aggatcatag ccgtcatctt tcagtccttt acagatttcc
     4441 atgcaggacg ggatcatact tcccactccc agcacagcga ttttttttcc tttgcggata
     4501 atctcacttc tgccatattc cacaggtgcc tgatattcct gaagcccctg atgcgcactg
     4561 cctctcggat agcggattgc aatcggtccg tcaaagctta ccgcaaatgc aagcatctct
     4621 tccagttctc tgtcattctt cggtgccaga acagtcatat tcggcatcat ggacaaataa
     4681 gacagatcaa agcagccctg atgcgtctcc ccgtctgcgc ctacaagccc tgccctgtca
     4741 acagcaaaaa tcacatgaag cttctgcata catacatcat gcaggatctg atctacagca
     4801 cgctgcagaa aagatgaata gatggcaaca acaggaacca ggccgccaag agccagaccc
     4861 gccgcaaaac tcaccgcatg ttcctctgcg attcccacat caaaaaatct gtccggaaat
     4921 ctcctggaga attccacaag tccggttcca tccggcatgg ctgcggtaat agctgtcagt
     4981 tttttatttt tctcaccaag agagaccagt gctttggaaa aaacatctgt ataacccggc
     5041 actgttttct tctgaagtac ccggcctgtt ttaatatcaa aaggacctgt tccatgaaac
     5101 ctgtccggat gggcacttgc aggttcgtat cctctgcctt tctttgtcag gacatgaaca
     5161 actaccggtc cctctacccg ttttgcttca ttaaagagtt tcatcatctg acgcatatta
     5221 tgaccgtcaa caggtcccag atatgtcagc cccatatttt caaacagcat tcccggaata
     5281 aaaagctgct tgacactgct tttggttttt ctcatagtat ctaccagagc agtccctacc
     5341 ttcggaacct tcttcagtgc ctttgtcaca cccatcttca ttcctgtata agcttctgca
     5401 gttctcagtg cacagataag tggacatgcc
//
LOCUS       DBMDXG010000101         5403 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3758_length_5403_cov_1.303848, whole genome shotgun sequence.
ACCESSION   DBMDXG010000101 DBMDXG010000000
VERSION     DBMDXG010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3758_length_5403_cov_1.303848"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(454..711)
                     /locus_tag="ACZPD3_04190"
     CDS             complement(454..711)
                     /locus_tag="ACZPD3_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HIR1415238.1"
                     /translation="MFTEECINTFLENQEQLFPQAVAESYEAAEAFLEDCMAQVVDSI
                     EEVREYLEESGADVEGMSDEELEDASEVFALPDGKYLIVEG"
     gene            complement(713..1516)
                     /locus_tag="ACZPD3_04195"
     CDS             complement(713..1516)
                     /locus_tag="ACZPD3_04195"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1415239.1"
                     /translation="MIKLVASDLDGTLLLHKAQSLPEEIFSLIRQLEELGIMFVAASG
                     RQYPNMTKLFAPVASEISYISENGALAVDHGEVLYQDSFDRKLAGEIISAILEKKDAE
                     FTCSAKDYHYMMPKTKRFHDHMLYEVKNECRFVNSMEEMTAPIMKLAVFEPAGLTEES
                     VKYWMDRFGKECVVVTSGNEWIDFIPFGTNKAKGIREYQKRYHISPEECIAFGDEYND
                     IEMLKAVKYGFAMEHSKEGVRAATSYMTKQVEPVLEKLIRAKGKIEEVI"
     gene            complement(1509..2174)
                     /locus_tag="ACZPD3_04200"
     CDS             complement(1509..2174)
                     /locus_tag="ACZPD3_04200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1415240.1"
                     /translation="MIKACIFDLDGTLADTLDSMAYVTNIIMEKFGLKTLPVDNFRYY
                     SGEGANMLIRRALKDAGDPELAHYDEGQKLYREMFEADPMYKVVPYKGMPETLKKLKE
                     HGMKLAVCSNKPHPAAVKVIAQLFDGEFDMVVGQSEAIRRKPAPDGPLMVAEKFGVKP
                     EECMYVGDTSTDMKTGKAAGMYTVGALWGFRDRKELNENGADLVAEKPTDLVKICEEH
                     GND"
     gene            complement(2181..2774)
                     /locus_tag="ACZPD3_04205"
     CDS             complement(2181..2774)
                     /locus_tag="ACZPD3_04205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR1415241.1"
                     /translation="MRKIILASASPRRKELLERAGVDFEVLPASGDENRISDNPGEAV
                     KQLASDKAASVIRTMKDSADGTIVIGSDTVVVFENVILGKPHDTEDAVNTLKKLQANT
                     HQVYTGVSVWEKKEKVWTEHTFYESTDVTFYPVSDEEIREYVATGEPMDKAGSYGIQG
                     LFGIYVKGINGDYNNVVGLPVARLFYEMKKSGINLRG"
     gene            2994..3389
                     /locus_tag="ACZPD3_04210"
     CDS             2994..3389
                     /locus_tag="ACZPD3_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415242.1"
                     /translation="MAKINKYWGYVAIGALTAAAAGAVAALIAKKGSCKEDFDFEDDF
                     DDFDLTDEKETAKEDQEPAEDFQSWEHTGEQSATDDTENEEAADTDDSEKSSDKAAEV
                     EIKDAEEEEDADEPENFEETMDAASESEE"
     gene            complement(3499..5166)
                     /locus_tag="ACZPD3_04215"
     CDS             complement(3499..5166)
                     /locus_tag="ACZPD3_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR1415243.1"
                     /translation="MENETVSKNFIENIIDKDLAEGVYDTVHTRFPPEPNGYLHIGHA
                     KSILLNYGLAQEYNGKFNMRFDDTNPTKEKSEFVESIKADIKWLGADWEDRLFFASDY
                     FGQMYEAAVKLIQKGKAYVCDLTADQIREYRGTLTEPGKESPYRNRSVEENLQLFEEM
                     KEGKYADGEKVLRAKIDMASPNMNMRDPVIYRVAHMTHHRTGDTWCIYPMYDFAHPIE
                     DAIEGITHSICTLEFEDHRPLYDWVVKELEYPQPPKQIEFAKLYLTNVVTGKRYIKKL
                     VEEGIVDGWDDPRLVSIAALRRRGFTPESIKMFVELCGVSKANSSVDYAMLEYCIRED
                     LKLKRPRLMAVLDPIKLVIDNYPEGEVEYLEAPNNMENEKLGTRKIPFGRELYIERED
                     FMVDPPKKYKRMFPGTEVRLMNAYFVTCTGYEADEDGTVRVVHCTYDPATKGGNAPDG
                     RKVKGTIHWVEASQAGKAEVRLYENIVDEEKGVYNKEDGSLNVNPNSLTKVTAYVEPA
                     LMEAKGYDSFQFVRTGFFCADIHDSKEGAPVFNRIVSLKSSFKLPKA"
BASE COUNT         1449 a         1324 c          906 g         1724 t
ORIGIN      
        1 ctgcaagtcc cttaatctta agcttaccgc ccaattctcc tgacggagct gccgcaaaaa
       61 ccatggatgg aatcattgaa ataaccatca tcacagtcat tacaactgca agaaatctct
      121 ttctctccat ctgtagtcct ccttttcatt tataataaga tttgtaatta ttcagcagcc
      181 actctgaagg tactgaataa ttacagttct gtgatgcttc tgtgtctgtc tgcccttttc
      241 attcccatca cagaccacaa catcgttttt tctctgtata aaagcattat agcatcacat
      301 tttccggaaa tgtaatctgt attttctccc ttaaaacaac ttcagcccct gagtatcaga
      361 gttcaatttt ctccaatatt cagggaccga actgtgtttt acagttattt ctccgatata
      421 tcacgtgata tccataattt gaccgaacag tttttatcct tctactatca gatattttcc
      481 atccggcaga gcaaatactt cacttgcatc ttccagttct tcatctgaca ttccttccac
      541 atcagcgcct gactcttcca gatattctct cacttcttca atagaatcca caacctgcgc
      601 catgcaatcc tcaaggaatg cttccgctgc ttcatatgat tctgctaccg cctgtggaaa
      661 aagctgctcc tgattttcaa gaaacgtatt aatacattct tctgtaaaca atttaaataa
      721 cctcctcaat ctttcccttt gcccggatca gcttctcaag gaccggttca acctgttttg
      781 tcatatagga cgtagctgcc cgtaccccct ccttggaatg ctccatggca aagccatatt
      841 ttaccgcttt aagcatctct atatcattat attcgtctcc gaacgcaata cattcttccg
      901 gcgaaatatg atatcttttc tgatattctc tgattccttt tgccttgttt gttccaaaag
      961 gaataaaatc aatccactca tttcccgaag tgacaacaac acattctttt ccaaatctgt
     1021 ccatccagta tttcacagat tcttctgtaa ggccggccgg ttcaaacaca gccagcttca
     1081 taataggagc cgtcatctct tccatgctgt ttacaaatct gcattcattt tttacttcat
     1141 ataacatatg gtcatgaaat ctctttgtct tcggcatcat gtagtggtaa tcctttgcag
     1201 aacaggtaaa ctcggcatcc ttcttttcca ggattgcaga aataatctcc cctgccagct
     1261 ttctgtcaaa agaatcctga tacagtacct ctccatgatc cactgccagc gcaccattct
     1321 ccgaaatata ggaaatctct gaagcaacag gtgcaaacag ctttgtcata ttcggatatt
     1381 gccttccact ggctgctaca aacataatcc ccagttcctc taactgcctg atcagcgaaa
     1441 aaatttcttc aggcagagac tgtgccttat gtaaaagtaa cgtcccatcc agatcacttg
     1501 caaccaactt aatcatttcc atgttcctcg caaattttta ccagatctgt cggtttctca
     1561 gcgacaagat ctgctccatt ctcattcagt tcttttcggt cacgaaagcc ccatagcgca
     1621 cctactgtat acattcctgc tgctttaccg gtcttcatat ctgtacttgt atctcccaca
     1681 tacatacatt cttccggttt tacaccaaat ttttccgcaa ccataagcgg accatcaggt
     1741 gccggtttcc tgcggattgc ttcactctga ccaactacca tatcaaattc cccatcaaaa
     1801 agctgcgcaa ttactttcac agctgcagga tgaggtttat ttgaacaaac tgccagcttc
     1861 attccatgtt ccttcaattt ctttaaggtt tccggcattc ccttataggg aactacttta
     1921 tacatcggat ctgcttcaaa catttcccgg taaagcttct gtccctcatc ataatgcgca
     1981 agttccggat cccccgcatc cttaagtgcg cggcggatca gcatatttgc cccttctccg
     2041 ctgtaatatc tgaaattatc caccggaagt gttttcaacc cgaatttttc cataataata
     2101 ttagtcacat aagccatgga atccagcgta tcagccagtg ttccatccaa atcaaatata
     2161 catgctttaa tcatcttttg tcatcctctt aagttaattc ctgatttctt catttcataa
     2221 aataatctgg caaccgggag tcctaccaca ttattgtaat ctccgttgat cccctttaca
     2281 taaattccga aaagtccctg gatgccatag cttccagcct tatccatagg ctctcctgta
     2341 gcaacatact ctctgatctc ctcatctgaa accggataaa aagtcacatc tgtactttca
     2401 taaaaagtat gttctgtcca gactttttct ttcttttccc atacagaaac gccggtatat
     2461 acctgatgag tatttgcctg aagtttcttt aatgtattca ctgcatcctc tgtatcatga
     2521 ggtttgccaa ggatcacatt ctcaaacact accactgtgt cagatccgat cacaattgtt
     2581 ccgtcggcac tatctttcat agtgcggata acagaagctg ctttatccga agccagctgt
     2641 tttactgcct ctcccggatt gtctgagata cgattctcat cacctgatgc aggcaacact
     2701 tcaaaatcta cgccagcccg ttccaacagt tcttttcgtc tgggtgaagc agatgccaaa
     2761 ataatttttc tcataataac ctcttctttc ctgtcatagt ataccttgta tctttttggt
     2821 atgcaactgc tttcattaac tctttccata aaatttcgct tttcttttta tctgaaaagt
     2881 attaaaataa caataaccta tagctgaata aaatggtgtc aggagtaaaa gatattttta
     2941 tcctgactcg ctgtcaaaaa taggaaaata tcagaaagga gtttatgatt atcatggcta
     3001 aaattaataa atactgggga tatgttgcaa taggtgccct tacagcagct gctgccggtg
     3061 ctgtagctgc ccttattgcc aaaaaaggat cctgtaaaga ggattttgat tttgaagatg
     3121 actttgatga tttcgatctg actgatgaaa aggaaactgc taaggaagat caggaacctg
     3181 ccgaagattt ccagtcctgg gaacatacag gcgaacagtc tgcaacagat gacaccgaaa
     3241 acgaagaagc tgctgataca gacgattctg agaagtcttc cgataaagct gctgaagttg
     3301 agattaaaga tgcagaagag gaagaagatg ccgatgaacc cgagaatttt gaagagacta
     3361 tggacgctgc atctgaatct gaagaataaa cgttaccgtt cacagtaacg taaccaaaaa
     3421 ggaatgttac ttcacgatta tacaatcgta gaagcaacat tcccttttta ttctgattat
     3481 attactattt cagtctcatt atgctttcgg aagtttaaat gagctcttca gagacacgat
     3541 acggttgaat accggtgcac cctctttaga atcatggata tctgcacaga aaaatcctgt
     3601 tcttacgaac tggaagctgt catagccttt tgcttccata agtgctggtt ctacatatgc
     3661 agtaactttt gtcaaagagt tcggatttac attcagagaa ccatcttctt tgttgtatac
     3721 acccttctcc tcgtctacga tattctcata gagacgtact tctgcttttc ctgcctgact
     3781 tgcttctacc cagtgaatgg ttccttttac ttttctgcca tccggcgcat tgccaccctt
     3841 tgttgcagga tcataagtac agtgtacaac acgtacagtt ccatcttcat cagcttcata
     3901 tcctgtacag gttacaaaat atgcgttcat aagacgaacc tctgtgcccg ggaacattct
     3961 cttatatttc ttaggcggat ctaccatgaa gtcttctctc tcaatataga gttctctgcc
     4021 aaacggtatt ttacgggttc ccagtttttc attctccata ttgttcggag cttccagata
     4081 ttctacttcg ccttccggat aattatcgat cacaagcttg atcggatcaa gaacagccat
     4141 cagacgtgga cgtttcagtt tcagatcctc acggatgcag tattcaagca ttgcatagtc
     4201 aacagagctg tttgctttgg atacaccgca gagttctaca aacattttga tggattccgg
     4261 tgtaaatcca cggcggcgca gtgctgcgat ggaaaccaga cgcggatcat cccatccgtc
     4321 tacgatgcct tcctctacca atttcttgat ataacgttta cctgtaacta catttgtcag
     4381 atacagtttt gcaaactcaa tctgcttcgg cggctgtggg tattccaatt cttttacaac
     4441 ccagtcatac agtggtctgt gatcctcaaa ctccagagta cagatggaat gtgtgattcc
     4501 ctcaatcgca tcctcgatgg gatgggcgaa atcatacatt ggatagatgc accatgtatc
     4561 acctgttctg tggtgagtca tatgagcaac acgatagata accgggtcgc gcatattcat
     4621 gttcggagat gccatatcga tcttggcacg aagcactttc tctccgtctg catatttgcc
     4681 ttctttcatc tcttcaaaaa gctgcaggtt ctcttctacg cttctgtttc tgtatggact
     4741 ttctttaccc ggctctgtaa gagttccacg atattcacgg atctgatctg ctgtcaggtc
     4801 acatacatag gctttgcctt tctgaatcag ctttacagca gcttcataca tctgtccgaa
     4861 atagtcagat gcaaaaaaca gtctgtcttc ccagtctgca ccaagccatt tgatatctgc
     4921 cttgatggat tctacaaact cactcttttc tttggtcggg tttgtatcat caaaacgcat
     4981 gttgaacttg ccgttatatt cctgtgcaag accatagttt aaaagaatag atttcgcatg
     5041 accgatatga agatatccat tcggttccgg cgggaaacgc gtatgcacgg tgtcataaac
     5101 accctctgct aaatctttat caatgatatt ctcaataaaa tttttggaaa ctgtttcgtt
     5161 ctccatcttt ttccctcctg ttgtcgtatt ttatactgtt ttatcacaca tctatatata
     5221 aaaaatacaa ctgaatattt tttatgtcga atgaatttat attatcattg gaaattccat
     5281 ctgtcaaccg tttacacaac agtgacaggc cttatatttc tcagacgtta ctgttcactc
     5341 cgttcacagt aacgtagcca aaattcattc cagattgcct gcggcaatgg aattttggct
     5401 tgt
//
LOCUS       DBMDXG010000102         5315 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3833_length_5315_cov_1.788262, whole genome shotgun sequence.
ACCESSION   DBMDXG010000102 DBMDXG010000000
VERSION     DBMDXG010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3833_length_5315_cov_1.788262"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="ACZPD3_04220"
     CDS             <1..232
                     /locus_tag="ACZPD3_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415244.1"
                     /translation="ISIILGRRYVTKEAKNLYLTEIGEVVNSMKQSSFYDKTTVRHQF
                     DRKCKLALDGEVVDYDRHMDYRRKNEVLFSII"
     gene            603..1034
                     /locus_tag="ACZPD3_04225"
     CDS             603..1034
                     /locus_tag="ACZPD3_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1415245.1"
                     /translation="MTFNKAKAEKKWRKWKETEEEKLRQLGMDEESIRKLRQMDWEDF
                     KEERRYREHLVESRGELEHKAVEEASQIVGDIRQLFDSVEDERVLHILLEADKTTLRI
                     LLLKMWGFSIKEIAYQMEIPEQTVYTKINRLRKKIKKIKKK"
     gene            1235..1513
                     /locus_tag="ACZPD3_04230"
     CDS             1235..1513
                     /locus_tag="ACZPD3_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415246.1"
                     /translation="MILDSVCSVCFRSERSLIAVNKIIADLSPRASKGDNDTNFCQKS
                     RGWTPSCNQQDFQEICERCKAKHPPVMFPSTAWQSGIRGVEDKYGDRE"
     gene            1626..3596
                     /locus_tag="ACZPD3_04235"
     CDS             1626..3596
                     /locus_tag="ACZPD3_04235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR1415247.1"
                     /translation="MFYKVEYQKRAHQEVEKIFRVLLPEQGLAVREEQIRLCHEMLDT
                     LLEGKIALCDAGVGIGKTYAYLVACVLMRKYFVLAGRYVPLDQRPVVITTSSVALQKA
                     ILSEYIPFLSRVLVEHGIIQSPLRAVIRKGKEHFVCDNRLEQRLEAVQHKQKNAVQRE
                     ALLSLRKHYDMDEVQGISGFDRRLVCVPKFCPRECPGRAACRYQRYLEEAKNKEIFIQ
                     ICNHNYLLADAMHRSKDYRPLLADYRALIVDEAHQLPEAAKQMYGKNLCYDDIREIGY
                     YLEREYQNTEARLLKGVMKNVFRIIAENHVSQNGMRSEFRATEECTLYLYEGIQIVQE
                     LTERLRGNIPKWVISRLEEAGKVMECFFRQDGRYVLYLKVDQDHLPILCAASREIPKL
                     LQDMLWSREHSAILTSGTLKAGKGFERTKQVTGLSGLEKVQEYVADSPFSYERNCMLF
                     LPKTLKRCKRGSREEAKMVANHIHSLICSTYGHTLVLFTSYSLMGNVYQLLRDELPFP
                     MIEVWRHTQEEILRFKTMENAVLFAAGSCWEGVDFPGDMVSSLIIVKLPFAVPDPISE
                     AEKEDYETLEEYIGEIIVPDMQKRLRQGFGRAIRTETDTCVVSILDYRAVRGGKYYED
                     VVCALPACLMAEDMKEVQAFIRSRKGADYYLS"
     gene            3764..4882
                     /locus_tag="ACZPD3_04240"
     CDS             3764..4882
                     /locus_tag="ACZPD3_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1415248.1"
                     /translation="MAQYVVGMQMEKKEKFYFIREKESMDIVLLPSKYLMHKKRSKIS
                     PNTIRRSAFALSYYLNFMDENQLHLENVYQMKYAEQHEHFTDFLIWLKAGEHSREDYS
                     KLPNNETCNAYLKEVFRFYNFMERENGQKESLKVLSDAQLIVRNSIGVRRVLNRKSFH
                     GYLKEKGHQGKTIEQDKIVSLLQECANCRDQVLLLLLAETGFRIGELLGIRYAEDIDY
                     KNHSLYVHFREDNENDARAKNAEFRRAKISEATFEILMFYIEDYKELILKQEYLFINV
                     SGDYVGKPFKTSGVYAMLRRLERKTGIKASPHMLRHYFANARRKDGWKLELISQALGH
                     RNIETTMKYLNITDEELIKVSDEFYSRHQAMYGVQDLL"
     gene            4899..>5315
                     /locus_tag="ACZPD3_04245"
     CDS             4899..>5315
                     /locus_tag="ACZPD3_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR1415249.1"
                     /translation="MSALVLLRTEQQENIRYLHEQLMTGGILQKELWEEAERYLIQQE
                     IYDITQIEEEDLRSYKKMLKDSGRFTQKQVKERSSALRKIHGYWLEKEYGELITEIQE
                     CVVTDEVYKGNVRDFLIQQGIHHIKDIDYTVRERYET"
BASE COUNT         1772 a          844 c         1357 g         1342 t
ORIGIN      
        1 gatttcaatc atacttgggc gcagatatgt gacaaaagag gcgaaaaatt tatatttgac
       61 ggaaattggg gaagtggtga actctatgaa gcaatcttct ttctatgaca aaacaacggt
      121 tagacatcag tttgatagga aatgcaaact ggcactggat ggcgaagtgg tagattacga
      181 caggcatatg gattatcgga gaaagaatga agtactgttt tcaattatct gaatgccagg
      241 caggagaatt ttcggtattg gatgaatata atctggaaat atattgactt caagtaatga
      301 gttaggacat tgaagtaaaa gatgggttga ttgttgagac tctgcgaatt cttataaaaa
      361 gaagtgaaat gtaattcttc tgtcgtattt tctaggcaca caaaggggca gtacatattt
      421 taaagaaaac tacaaaagaa aagagtctaa cttcagaaag agcgtttggt ggtgtcgcaa
      481 ttctagacag gattgtggca cttttttttt cgacaaaatt cgaagcgaat ttacagagta
      541 aagctagagc accgccacac tccgatttga tttcaaaaat tcagatcgga ggttaagaaa
      601 ggatgacttt taataaagca aaggcagaga aaaagtggcg gaaatggaaa gaaacagaag
      661 aggaaaagct tcggcaactc ggaatggacg aagaatcaat aagaaaactt cggcagatgg
      721 attgggaaga tttcaaggag gaacgcagat accgggaaca tctcgtagaa agcagaggag
      781 aactggaaca taaagcagta gaagaagcgt ctcagatagt tggggatatc cgacagctat
      841 ttgacagtgt agaagatgaa agggttttac atattctgtt ggaggcagat aaaacaacac
      901 tgcggattct attactaaag atgtggggat tctcgattaa agaaattgca tatcaaatgg
      961 agatcccgga acagactgtt tataccaaaa taaatcgttt acggaaaaaa ataaaaaaaa
     1021 ttaaaaaaaa gtgaataaaa acagggaatc tcatcggcta tgtatatgta agggggaaaa
     1081 atctcccttg catgtacatt gaaaatcaca tatcagcatt acaggtacgt tcccaaggat
     1141 agtagcgtga cagcataagc gccaagacgg acgggagcag aactggatac aggagaaaga
     1201 aacatcttca gtttcaaaag aaacgaagaa atggatgatt ctggattctg tatgcagtgt
     1261 ctgttttcgt tctgagcgat cccttatagc tgtgaataag atcatagcgg atctttcgcc
     1321 aagagcatct aagggggata atgatacgaa tttctgccag aagagcagag gatggactcc
     1381 aagttgtaat cagcaagact ttcaggaaat ctgcgagcgg tgcaaagcga agcatccgcc
     1441 tgtaatgttc ccaagcactg cgtggcagtc aggtatccgg ggagtcgagg acaaatacgg
     1501 agacagagaa taaaagaaga tgaaatcaaa gaagagtgtg tcgttgatat cagatacatg
     1561 cggctgtgag taacatcata gccgtattga tgtggtatta acgtcatgcg gaaagaagaa
     1621 atcgtatgtt ttataaagtt gaatatcaga aaagagcgca tcaggaggtt gaaaagattt
     1681 tccgggtatt attgccggaa caagggttgg cagtaaggga agaacagatc cgcttatgcc
     1741 atgaaatgct ggataccctg ctggaaggaa aaattgcttt atgtgatgca ggagttggta
     1801 ttggaaaaac ctatgcttat ctggtagcct gcgtactgat gcggaaatat ttcgtactgg
     1861 caggaagata tgtaccattg gatcagcgtc cggtggtgat taccacttcc agcgttgcac
     1921 tgcagaaagc aattctttct gaatacatcc catttctttc cagagtgttg gtagagcatg
     1981 gaattattca gtcaccgctt cgtgcggtaa tacgcaaggg gaaagaacac tttgtctgtg
     2041 acaacaggct ggaacaacgg ttagaagccg tccagcacaa acagaagaat gcggttcaga
     2101 gagaggcgct gttatctttg cgaaagcatt atgatatgga tgaagtgcag ggaataagcg
     2161 gttttgatcg caggctggtc tgtgtaccga aattctgtcc aagggaatgt cccggaagag
     2221 ctgcctgcag ataccagagg tatctggaag aagcaaagaa caaagaaatt tttattcaga
     2281 tttgtaacca taattatctg ctggcagatg ccatgcaccg cagcaaagat taccgtccat
     2341 tgttagccga ttaccgggca ttgattgtgg atgaggccca ccagcttccg gaagcagcaa
     2401 agcagatgta tgggaagaat ttatgctacg atgatattcg tgaaattgga tattacttgg
     2461 aacgggaata tcagaataca gaggcaaggc tgctaaaagg tgttatgaag aatgtattta
     2521 gaattattgc ggagaaccat gtgtctcaga acgggatgcg gtcagaattc agagcaacgg
     2581 aagaatgtac attgtatcta tacgaaggaa ttcagatagt acaggaattg acggagaggc
     2641 tcaggggaaa tattcccaaa tgggtaatca gcaggctgga agaggcagga aaggtgatgg
     2701 aatgcttttt ccgtcaggac gggaggtatg ttctttacct gaaagtagat caggaccatc
     2761 tccccatttt gtgtgccgcc agccgggaaa tcccaaaact tctgcaggat atgttgtgga
     2821 gccgggagca ttcagccatc ctgaccagcg gaaccttaaa ggcaggaaag ggatttgaaa
     2881 gaacaaaaca ggttaccggg ctatccggtt tagagaaggt acaggaatat gtggcagatt
     2941 ctccgttttc ttacgaaaga aattgtatgc tgtttctgcc aaaaacattg aagagatgta
     3001 aacgggggag cagggaagag gcaaagatgg tggcaaatca tatccattcc ctcatctgtt
     3061 ccacttacgg gcatacgctg gtgctgttta catcctatag cttgatggga aatgtgtacc
     3121 agcttcttcg ggatgagctt ccttttccga tgatagaggt atggcgtcat acgcaggaag
     3181 aaatcctgcg gtttaagaca atggaaaacg cagtgttgtt cgcagccggc tcttgttggg
     3241 aaggggtaga ttttccggga gacatggtgt cttccctgat tattgtaaaa cttccctttg
     3301 ctgttccaga tcctatcagt gaggcagaaa aagaggatta cgaaacactg gaagaataca
     3361 tcggggaaat tattgtgccg gatatgcaga aaagactccg gcagggattt ggacgtgcca
     3421 tccgtacaga aacagacact tgtgtggtat caattttaga ttaccgggca gtgagaggcg
     3481 gaaaatatta tgaagatgtg gtatgtgctt tgccagcctg cctgatggca gaagacatga
     3541 aagaagtaca ggcttttata cggagcagga agggagcgga ctattatctg tcctaatcat
     3601 gttccggtat tgttggttat gcttcgtttg tgccggggat tgcagagaat ctttagtaca
     3661 gtttcttaga agaaatccca cagtttgttg gtattgatga atagctattt gggcatgaag
     3721 tgggtatact gtgtatagcg ttagaggaaa ggaagtgaag aaaatggcac aatatgtagt
     3781 agggatgcag atggagaaaa aggagaaatt ctattttatc cgggaaaaag aaagcatgga
     3841 tattgtacta ttaccttcca aatatctaat gcacaaaaag aggtcgaaga tatcaccgaa
     3901 caccattcga aggagcgcat ttgccctatc ctattatctg aactttatgg atgaaaacca
     3961 actgcattta gaaaatgtgt atcagatgaa gtatgcagaa cagcatgaac attttacaga
     4021 ttttttaatc tggctgaaag caggtgagca tagcagagaa gattattcaa aacttccgaa
     4081 taatgaaaca tgtaatgctt acttaaaaga agtgttccga ttttacaatt ttatggaacg
     4141 ggaaaacggg cagaaagaaa gtctgaaggt gttatctgat gcacagctaa ttgtgcggaa
     4201 ttccatcggt gtcagaaggg tacttaaccg gaaaagtttt catggttatt tgaaagagaa
     4261 aggtcatcaa gggaaaacta ttgagcagga caagatcgta agtttgctgc aggaatgtgc
     4321 gaactgccgc gatcaggttc tgcttttatt attagctgaa acaggatttc gcatcgggga
     4381 acttctggga atccgttatg cagaggatat cgattacaaa aatcatagcc tttatgtaca
     4441 cttcagagaa gataacgaaa atgatgcaag agcaaagaat gcggaattcc gcagagcaaa
     4501 aatcagtgaa gcgacatttg aaattttaat gttttatatt gaagattata aagaactgat
     4561 tttaaaacag gaatacttat ttattaatgt ttccggagac tatgttggga aaccgttcaa
     4621 aaccagtgga gtgtatgcta tgctacgacg gctggaaagg aagacaggaa ttaaggcttc
     4681 tccacatatg ctaagacatt attttgcaaa tgcaaggcgt aaagacggtt ggaaattgga
     4741 attaatcagt caggcacttg gacatcggaa tatcgagacg accatgaaat atttaaatat
     4801 aacagatgaa gaactaataa aggttagcga tgaattttat agcagacatc aggccatgta
     4861 tggggttcag gatttgttat aacaaaggag gcggttaagt gtctgcacta gtattgctta
     4921 gaaccgaaca gcaagaaaat ataagatacc ttcatgagca gctaatgaca ggcgggatat
     4981 tgcagaagga attgtgggaa gaggcagaac gatatctgat tcagcaagag atttacgata
     5041 ttacacagat agaagaagag gatcttcgat catataaaaa aatgctgaaa gatagtggta
     5101 ggtttacaca aaagcaggtc aaggaacgat catccgcatt gcggaagatt catggttact
     5161 ggttagaaaa ggaatatgga gaactgataa cagaaataca ggaatgtgtg gtgacagatg
     5221 aggtatataa aggtaatgta agagattttt tgattcaaca gggaatccac catattaaag
     5281 acattgatta tacagttcgg gaacgctatg aaacg
//
LOCUS       DBMDXG010000103         5315 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3834_length_5315_cov_1.495397, whole genome shotgun sequence.
ACCESSION   DBMDXG010000103 DBMDXG010000000
VERSION     DBMDXG010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_5315_cov_1.495397"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2693)
                     /locus_tag="ACZPD3_04250"
     CDS             complement(<1..2693)
                     /locus_tag="ACZPD3_04250"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043038 - amino acid activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR1415250.1"
                     /translation="MKNINERNNFVFEFRPVTELFEEQVKLHPEKCAVVSGKESFTYA
                     QLNERANRIANALVEKGVRRETIVGVVLERCCDFYAVRQGILKAGGAFAVATPDYPDD
                     RIRYIFEDSGAPFIITTKEIAEERRELFAKLPCTVLLIEELLENENTENPQVKIGEHD
                     LCYCIYTSGSTGKPKGVLIEHINLANFVNPDPKNAETYGYVSRGSVSLSMAAMTFDVS
                     VLEEFLPLTNGMTAVIASDEEILNPLMLGELIVRNKVDIMTTTPTYLSNMIDLPQLEK
                     AVSQIKVFDVGAEAFPPALYDKIRRVNPDAYIMNGYGPTETTISCTMKVITDSRNITI
                     GTPNGNVKVYIVDKENKILPDGETGELVIAGLGVGRGYMNLPDKTEAVFIDLNGERAY
                     KTGDLARISPEGEIEFFGRIDNQIKLRGLRIELGEIEEVINSYEGIITSITLPVDNKF
                     LCCYFMADRQINTEELSAYASESLAHYMVPEVFVQLEKMPVTQNGKIDKKALPKPAAQ
                     PKNLKEPQTPMQKKIFEIVADVVENDFFGTDTSFYRAGLSSISAMKLCILISEEFGVT
                     VKTSDIHENNTVEKLEKYVMLAPKIRTYEKREVYPLTGSQKGIFAECMKNPESTVYNI
                     PFLFELESSVDVQKLSDAISQMIAAHPYLLTKVYLSDSGEMVQKPCEEAFVPEVVQTT
                     NEQFEKMKDELVRPFKLEKGRLFRAGIYVTEDRKYLFTDFHHILADGNSYDIIFEDID
                     RAYLGEKLEKESYTGFDAALDEEQQMKEGKYKKAEKYYDSIFEGIETESLPLPDCSGK
                     TPERGYLSMPVDTGESLVLSACEKLGVTPNILFTGVFGILMSRYSNSEESLFATIYNG
                     RNDSRLENTVCMLVKTLPVYCSCDPKVTIQAYMTELSEQILS"
     gene            complement(2707..4452)
                     /locus_tag="ACZPD3_04255"
     CDS             complement(2707..4452)
                     /locus_tag="ACZPD3_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415251.1"
                     /translation="MEERNNRLLNAKLNKYIVPGIMMSLALQLGNIVDTIFVSNLIGV
                     EAMSAVTLSLPVETLVQLTGYCLGIGGSIAVGNMLGKRDKEGASKLFSATFIVTLVVG
                     IIFSICALPAAEPVARLLVSGDGVLTGYTRDYIRVSMLGAPVIGIGLMMVNYLGVENH
                     PELASAYLIAANVINLVLDYIFLRYTPLGITGASLSTVLGFLFAMGIFVLYIRSDKRN
                     LHLILLKPGELGITKEAIITGVPMLIFMAANFVKALGLNTIIMNQLGEEGMAVFTVCD
                     NVLLIVEMLTGGIIGVIPNVAGILFGEKDYVGIRVLCKKMLKYSYILLAVIFVLIMLF
                     TEEITVMFGSGGGELGSHMVQALRIFALCVAPYLWNKFIISYYESIEETVIASFATFL
                     ENAVVVLPATLVGILVWKQIDGIGIDGIAAGFVATEIITAVAACIFRKIRHKNTSFYI
                     VPDKNPGINLDFSIKSTMEEAQTVHKRIIEFCQEQGASKSKANLAAVCAEEMTVNIIR
                     FGGKTSNWIDINLCLEDDLCRLRIRDNGVNFNPLEYQYDSEDFDIHGIELVKKVSKSM
                     DYIRAIDMNNTIISF"
     gene            complement(4571..>5315)
                     /locus_tag="ACZPD3_04260"
     CDS             complement(4571..>5315)
                     /locus_tag="ACZPD3_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR1415252.1"
                     /translation="PGRTVKWRYYGTRSSTESFWLGMEDSAAIYKGYIFMTDNGGNLM
                     CLDLNTLQLVWVQDTLDDSNSTPVLEIEKGHLYLYVSTSFRLGWRSYDTATVPVWKID
                     AETGEIVWHTDYECTTDDGVSGGVQSTIACGKNSLADYIYVTVAKTSDNASGVLACLR
                     KSDGSKAWEDSSSYAWSSPVCVYNKDGSGKVLYCNSTGDIRLLDGKTGKQEDVLSVSE
                     GVIEASPAVYDNYAVVGTRDCKIWGIRLQ"
BASE COUNT         1458 a         1353 c         1011 g         1493 t
ORIGIN      
        1 gacagaatct gctcagagag ctctgtcata taggcctgta tggtgacttt cggatcacag
       61 gaacagtaaa ccggcagagt ctttacaagc atgcagactg tattctccag tctggaatca
      121 tttcttccat tataaatagt tgcaaaaaga ctctcctcgg aattggaata tctgctcatg
      181 agaatgccga atacacctgt aaacagaatg tttggagtaa cccccagctt ctcgcaggca
      241 gaaagtacca gagactctcc ggtgtccaca ggcatcgaca gatatcctct ctcaggagtc
      301 tttcctgagc agtcaggaag aggcagggat tctgtctcaa ttccctcgaa aatactgtca
      361 taatatttct cggccttctt atattttccc tctttcatct gctgttcttc atcaagggca
      421 gcatcaaagc ctgtgtagga ttctttctcc aatttctcac ccagataggc ccgatcaata
      481 tcctcaaaaa taatatcata ggaattacca tctgccagga tatggtggaa atctgtaaaa
      541 agatatttcc tgtcttctgt cacataaatc cccgcccgga acagccgtcc cttttccagc
      601 ttaaagggac gcaccagctc atctttcatc ttttcaaact gctcatttgt ggtctgtacc
      661 acctctggta caaaggcttc ctcacaaggc ttctgcacca tctctccgct gtcacttaaa
      721 taaacctttg tcagcagata cggatgtgca gcgatcatct gagaaatggc atctgacagc
      781 ttctgaacgt caacagagct ttccagttca aacaggaacg gaatattgta taccgtactc
      841 tcaggattct tcatgcactc tgcaaaaata cccttctggg agccggtaag agggtaaact
      901 tctctctttt cataggttct gatcttcggt gcaagcatca catatttttc cagtttctcc
      961 accgtattgt tttcgtggat atcactggtt ttcacagtca ctccgaattc ctctgaaatc
     1021 agaatacaaa gcttcatggc actgatagaa gacagccccg ccctgtaaaa acttgtgtca
     1081 gttccaaaaa aatcattctc cacaacgtct gcaacgattt caaaaatctt cttctgcata
     1141 ggagtctggg gttctttcag attctttggc tgtgcagccg gctttggcag tgctttctta
     1201 tcaattttac cattctgtgt cacaggcatc ttttccagtt ggacaaaaac ctcaggaacc
     1261 atataatgag ccagggactc tgatgcatag gcagaaagct cctctgtatt gatctggcgg
     1321 tcagccataa aatagcagca gaggaatttg ttatctacag gcagagtaat gctggtaatg
     1381 attccctcat aggaattgat cacttcctcg atctcaccaa gctcaatcct cagaccgcga
     1441 agcttgatct gattgtctat tcttccgaaa aattcaatct caccctcagg gctgatcctt
     1501 gcaaggtcac cggtcttata agctctctcg ccgttaagat caatgaatac agcctctgtt
     1561 ttatccggca gattcatata acctctgcca acgcccagtc ctgcgatcac aagctctccg
     1621 gtctctccgt ccggaaggat cttattctct ttatctacaa tatatacctt cacattaccg
     1681 ttaggcgtac caatggtaat gtttctgctg tccgtaatta ccttcatggt acagctgatg
     1741 gtagtttccg taggtccata accattcata atgtaagcat ccgggtttac tctgcgaatc
     1801 ttgtcataca gagccggagg aaaagcctct gcacctacat caaacacctt aatctgagaa
     1861 actgcttttt caagctgggg cagatcgatc atattggaca ggtaagtagg agttgtggtc
     1921 ataatatcca ctttatttct gacgatcagc tcacccagca taagaggatt cagaatctcc
     1981 tcatcagagg cgatcaccgc agtcatcccg ttggtaagtg gcagaaattc ctccagaaca
     2041 gacacatcaa aggtcatggc tgccatggaa agagaaaccg atcccctgct gacataacca
     2101 taagtttccg catttttcgg atccggattt acaaaattgg caaggttgat atgttcgatc
     2161 agcacaccct tgggcttgcc tgtggaacca gaagtataga tacaataaca aagatcatgc
     2221 tccccgatct ttacctgagg attctctgta ttctcattct ccagaagttc ctcgatcaga
     2281 agtactgtac agggaagctt agcaaacagt tcccttcgtt cctctgcgat ctcctttgtt
     2341 gtgataataa aaggagctcc tgaatcctca aaaatatacc ggatcctgtc atcaggataa
     2401 tccggtgtgg caactgcaaa tgcgcctcct gccttcagga taccctgtcg cactgcatag
     2461 aaatcacagc atctctcaag aaccacacct acgatggtct cccgtctgac acctttttcc
     2521 acaagtgcat tggcaatcct gttagcccgt tcgttgagct gtgcataggt gaagctctct
     2581 ttgccggaga caactgcaca tttctctgga tgaagcttca cctgttcctc gaacaattct
     2641 gttaccggcc gaaactcaaa aacaaaattg ttcctttcat taatattttt cattttcatg
     2701 tacctcctaa aaagatatta tggtattatt catatcaatc gcacgaatat aatccatgga
     2761 ttttgatacc ttcttgacaa gttcaatccc atgtatatca aagtcttcac tgtcatactg
     2821 atattccaga gggttgaaat ttacaccgtt atctctgatc ctcagtctgc agagatcatc
     2881 ctccagacag aggttaatgt cgatccagtt ggaggttttt cccccgaatc gtataatatt
     2941 tacagtcatt tcctccgcac acactgcggc aagatttgcc ttgcttttag aagctccctg
     3001 ttcctggcag aactctatga tcctcttatg aaccgtttgt gcttcctcca tggtactctt
     3061 aatggaaaaa tccagattaa tccccggatt tttgtcagga acaatataaa agcttgtatt
     3121 cttatgcctg atttttctga aaatacaggc tgcaactgca gttatgatct cagtggcaac
     3181 aaatcctgct gcaattccgt cgataccgat cccgtcaatc tgtttccata caaggattcc
     3241 caccagggtt gcaggcagaa ctaccaccgc attctccaga aatgtggcaa aacttgcaat
     3301 gactgtctcc tcaatggact cgtaatagct gataataaac ttattccaca gataaggtgc
     3361 cacacacaaa gcaaagatgc gcagcgcctg gaccatatgg ctgcccagct cgccaccgcc
     3421 gcttccgaac atgacagtga tttcctcggt aaacagcata ataaggacaa agatcacagc
     3481 cagcaggatg taactgtatt tcagcatctt tttacagaga acccggattc ccacgtagtc
     3541 cttctctcca aaaaggattc ctgccacatt ggggatcaca ccgatgattc cgcctgtaag
     3601 catctcgacg atcagcagca cattgtcaca gacagtaaat actgccatac cttcctcgcc
     3661 cagctggttc atgataatgg tattcagtcc aagcgccttt acaaaattag ctgccataaa
     3721 gatcagcatt ggcacgcctg tgatgattgc ttcctttgtg attccaagtt cgcccggctt
     3781 tagcaggatc agatgaaggt ttcgcttatc agagcggata taaagcacaa aaattcccat
     3841 tgcaaacagg aaaccaagaa cagttgataa ggatgcacct gtaattccca gcggagtata
     3901 tctgaggaat atatagtcca gcacaagatt gatcacattg gctgcgataa ggtaggctga
     3961 tgccagctcc ggatgattct ccactccaag gtaattgacc atcatcagtc caattccgat
     4021 caccggtgcc cccagcatac ttacacgtat gtaatctctg gtataaccgg tcaggacacc
     4081 gtcacctgat accagaagtc tggcaacagg ttctgctgca ggaagggcgc agattgaaaa
     4141 aatgattccc accacaagtg ttactataaa tgtggcagaa aacaatttcg atgccccttc
     4201 tttgtctcgt ttgccaagca tgtttcccac tgctatggaa ccgccgatac caaggcagta
     4261 acccgtaagc tgaacaagcg tctctacagg caggctcagg gtaactgcag acattgcctc
     4321 cacacctatc agattactta caaatatcgt atccacaata ttccccagct gaagtgccag
     4381 agacatcata atccccggaa caatatactt gttcagcttc gcattcagca gtctgttatt
     4441 tctctcttcc atcgcttcct ctcctttgtt ttcagttttt tcaaaatatt gtgtcgtaaa
     4501 actgtaagat atttaaattt taccatttag tgcgataaat gaatgggaga aagcaaataa
     4561 atattgctat ttactgtaac ctgattcccc atattttaca gtctctggtg cctacaaccg
     4621 catagttatc gtagacagcc ggggaagcct ctatcacgcc ctcgctgaca gataatacat
     4681 cttcctgttt acctgtttta ccatcaagaa ggcggatatc acctgtactg ttgcaataaa
     4741 gaacctttcc tgatccgtct ttgttataaa cgcacaccgg agaagaccag gcatatgagg
     4801 aagaatcctc ccatgcttta gatccgtcag attttctcag acaggcaaga actccggatg
     4861 cattgtcaga ggtctttgcc actgtcacat atatataatc ggcaaggctg ttctttccac
     4921 aggcaatcgt agactgaact ccaccggaaa caccgtcatc agtagtacat tcataatctg
     4981 tatgccacac gatctcaccg gtttctgcat caatcttcca gacagggaca gtggctgtgt
     5041 cataacttct ccatccaagg cggaaggaag tacttacata cagatacaga tgtccttttt
     5101 cgatttccag aacaggcgtt gaattagaat catccaatgt atcctgaacc cataccagct
     5161 gaagagtgtt cagatccaga cacataagat ttcccccatt atcggtcata aatatatagc
     5221 ctttatagat tgccgcactg tcttccattc caagccagaa agactctgta ctgcttcttg
     5281 ttccatagta gcgccatttt acagttctgc ccgga
//
LOCUS       DBMDXG010000104         5298 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3849_length_5298_cov_1.369636, whole genome shotgun sequence.
ACCESSION   DBMDXG010000104 DBMDXG010000000
VERSION     DBMDXG010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_5298_cov_1.369636"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(23..1735)
                     /locus_tag="ACZPD3_04265"
     CDS             complement(23..1735)
                     /locus_tag="ACZPD3_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR1415253.1"
                     /translation="MEFSAVNTIWVLVGAALVFFMQAGFAMVETGFTRAKNAGNIIMK
                     NLMDFCIGTPVFWLVGFGLMFGAGNGFIGKIGGIATEAHYGSGMLPDGVPFWAFLIFQ
                     TVFCATSATIVSGAMAERTKFLSYCIYSLMISLVVYPVSGHWIWGGGFISQMGFHDFA
                     GSCAVHMVGGVAALIGAIILGPRIGKYTKDGKSKAIPGHNLTVGALGVFILWFCWFGF
                     NGASTVSMEGDAIVSAGKIFVTTNLSAAVATVTVMIITWVRYKKPDVSMSLNGSLAGL
                     VAITAGCDTVSPTSAAIIGIASGFIVVFGIEFIDKVLKIDDPVGAVGVHGLNGAFGTL
                     AVGLFSDGSGTDWKGLLTGGGFHGFGVQFTGMIITIAWVVVTMTIIFQVIKHTIGLRV
                     SAEEEIAGLDSKEHGLASAYDGFAMAGVPTPMGTSIKAPVVTARPSAPAESVPELPKE
                     GVHKLTKVVIITRQNKLDEFMQAMNEIGVTGITITNVLGCGVQKGAPSYYRGVEMEMN
                     LLPKVKIEIVVSLVPVQKVIETAKAVLHTGQIGDGKVFVYDIENVVKIRTGEEGYLAL
                     QDTK"
     gene            complement(2034..4133)
                     /locus_tag="ACZPD3_04270"
     CDS             complement(2034..4133)
                     /locus_tag="ACZPD3_04270"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR1415254.1"
                     /translation="MAQNIPELYGSLVFNDKVMRSKLPKDMYKALKKTIESGTHLELD
                     VANSVAVAMKEWATENGATHYTHWFQPMTNVTAEKHDSFISPTGDGQVIMDFSGKELV
                     KGEPDASSFPSGGLRATFEARGYTAWDPTSPAFIKDGTLYIPTAFCSYSGEALDKKTP
                     LLRSMQTLDKEATNLLHIIGNKDIKHVNTTVGPEQEYFLVDKELYKQRKDLVFCGRTL
                     IGAPAPKGQEMEDHYFGALKPRVAAYMHDLDVELWKLGIPAKTKHNEVAPAQHELAPV
                     FDTTNVAVDHNQLTMEVMKKVADKHGLVCLLHEKPFEGINGSGKHNNWSMITDTGVNI
                     LDPGKTPAENTQFLIFLTAVIKAVDEYADVLRISVASAGNDHRLGANEAPPAVVSVFL
                     GDELTEVLKSIENDEYFAGSRAVQMDIGAKVLPHFVKDNTDRNRTSPFAFTGNKFEFR
                     MLGSEASVANPNIILNTAVAECVHQFAEQLKDVPEDKMEDAIHELIKKTIIDHKCVIF
                     NGNGYTDEWIEEAEKRGLFNLKSTPDALPQWIADKNIELFTKYHIFTKEEIESRYEIW
                     LESYSKILNIESNTMVEMVQKDFLPSVFAYIDKVAATAVAKKSVVSDVSTASEGKLIK
                     ELSQLADEISTGLETLKADTAKALATEDPLANAKAYQTVVLSDMDELRKSVDAAETLI
                     PDALLPYPTYDKLLFSV"
     gene            4550..>5298
                     /locus_tag="ACZPD3_04275"
     CDS             4550..>5298
                     /locus_tag="ACZPD3_04275"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1415255.1"
                     /translation="MAGIKEECGVFGIYDLDGGNVVPSIYYGLTSLQHRGQESCGLAV
                     SDTKGERGNVKFHKDLGLVSEVLRQDVVRKYEGDIGIGHVRYSTTGASVAENAQPLVL
                     SYVKGTLALAHNGNLVNTPELKWELIQNGAIFHTTTDSEVIAFHIARERVHSKTVEEA
                     VLKTAKKIKGAYGLVVMSPRKLIAVRDPYGLKPLCLGKRGNAYVIASENCALTSVSAE
                     FIRDIEPGEILTITKDGLKSNKELASAAAKRA"
BASE COUNT         1518 a         1102 c         1153 g         1525 t
ORIGIN      
        1 tacctgattg ttgataaata atttactttg tatcctgcag tgcgagataa ccttcttcgc
       61 ctgtacggat cttaacgaca ttttcaatgt catatacaaa tactttacca tcaccaatct
      121 gaccggtatg gaggactgcc ttggcagttt cgataacttt ctgtacaggt acgagactta
      181 caacaatctc aattttgacc ttaggaagaa gattcatttc catctcaaca ccacgatagt
      241 aggatggagc acctttctgt acaccgcatc cgagaacgtt cgtaatagtg atacctgtaa
      301 caccgatctc attcattgcc tgcataaatt catcaagctt attctgacgg gtgatgataa
      361 caacttttgt aagcttatga acgccttctt tcggaagttc aggaactgat tcagcaggag
      421 cagaaggtct ggctgtgaca acaggagctt ttatggaagt tcccatagga gtcggaacac
      481 cagccattgc gaaaccgtca taagcacttg caagaccatg ttctttggag tcaagacctg
      541 cgatctcttc ttcagctgaa acacgaagtc cgattgtatg tttgattacc tggaagataa
      601 ttgtcatagt aacaacaacc catgcgattg tgatgatcat accagtgaac tgaacaccaa
      661 atccgtggaa gccgccgcct gtaaggagtc ctttccagtc tgtgcctgat ccgtctgaga
      721 aaagaccaac tgcaagggta ccgaaagcac cgtttaaacc atgaacaccg acagcaccaa
      781 cagggtcatc aattttcaga accttgtcaa taaattcaat accaaatact acgataaaac
      841 cggaagcaat accgatgatt gctgcggatg taggagaaac tgtgtcgcat cctgctgtaa
      901 ttgctacaag tcctgcaaga gaaccgttca gagacatgga aacatccggt tttttgtaac
      961 gtacccatgt gatgatcata acagttacag ttgcaacagc tgctgaaagg ttagtagtaa
     1021 cgaagatttt accggcactt acgattgcat cgccttccat acttactgtg gaagcaccgt
     1081 taaatccgaa ccagcagaac cagaggataa atacacctaa agcgccaact gtaaggttgt
     1141 gtcccgggat agcctttgat ttgccgtcct ttgtatattt accgatacga ggtccaagga
     1201 tgatggcacc gatcagagct gccacaccac ctaccatatg tactgcacag gaacctgcga
     1261 aatcgtggaa tcccatctgg ctgataaatc caccgcccca gatccagtgt ccggataccg
     1321 gatatacaac aagactgatc ataaggctgt agatgcaata ggaaaggaac ttggtacgtt
     1381 ctgccattgc accggaaaca attgttgcgg atgttgcaca gaatactgtc tggaagatta
     1441 agaatgccca gaaaggaact ccgtcaggaa gcattcctga accatagtgt gcttctgtgg
     1501 caataccacc gatctttccg ataaatccat tacctgcacc aaacatcaga ccgaagccga
     1561 ccagccagaa gaccggggtt ccgatacaga agtccatcag gtttttcata ataatgttac
     1621 ctgcgttttt ggctcgtgtg aagcctgttt ctaccattgc gaatccggcc tgcataaaga
     1681 agaccagcgc ggcgccgacc agcacccata ttgtgtttac tgcggaaaat tccataataa
     1741 tattcctccc tcataaaaat ttcctcatat tttataaaat gcaaaaagcg cgatgcctgg
     1801 aaatgtagcc agttcatcgc gcttttgcga atataaaatc catatttata gtgtattgca
     1861 taactgatgt aatagaaaag acatttcgta tgactgtatg caatgcagtt taaaatcaaa
     1921 attatatgta aaacaggaga acatcaaaat atcgtgtgat taatggatga ggaaatcgat
     1981 aatgaattat gaagtagagt tcataaagat attcccctgt tattacctgt taattaaacg
     2041 gaaaacagaa gcttgtcata tgtaggatat ggaagcagtg catccggaat cagagtttct
     2101 gctgcatcta cagactttct gagttcatcc atatcagaaa gaacaactgt ctggtatgct
     2161 tttgcattag ccagaggatc ttctgtagca agcgctttgg cagtgtctgc ttttaaggtc
     2221 tcaagacctg tggaaatctc atcagcaagc tgtgacagct ctttgatcag ttttccttca
     2281 gaagctgtgc tgacatctga aacaactgat tttttagcaa cagcagtagc tgcaactttg
     2341 tcgatatatg cgaatacaga tggaaggaaa tccttctgta ccatttcaac catagtattg
     2401 gactcaatgt taagaatctt ggaataggat tccagccaga tctcataacg ggattcaatt
     2461 tcttcttttg tgaagatatg atatttggta aagagttcaa tatttttatc tgcgatccac
     2521 tgaggaagag catctggtgt agatttcagg ttaaagagac ctcgtttctc agcttcttcg
     2581 atccattcgt ctgtataacc gtttccgtta aagataacac atttatgatc aataattgtc
     2641 ttcttgatca gttcgtggat tgcatcctcc atcttgtctt ccggtacatc ctttaactgt
     2701 tctgcaaact ggtgaacaca ctcagcaaca gcagtgttta agataatgtt cgggtttgca
     2761 acagaagctt cggaaccaag catacggaat tcgaatttgt tacctgtaaa tgcgaatggt
     2821 gaagtacggt ttctgtctgt attgtctttt acgaaatgtg gaagtacttt tgcaccgata
     2881 tccatctgga ctgcgcggct tcctgcaaaa tattcgtcat tttcgataga ttttaatact
     2941 tctgtcagtt catcaccgag gaatacggat acaacagcag gcggagcctc gtttgcacca
     3001 agacggtgat catttcctgc acttgctaca gaaatacgaa gtacatctgc gtattcatca
     3061 actgctttga taacagctgt caggaaaatc aggaactgtg tattttcagc aggtgttttt
     3121 cccggatcaa ggatatttac accggtatct gtaatcatag accagttgtt gtgtttacca
     3181 cttccgttga ttccctcaaa tggtttctcg tgaagaaggc atacaagtcc gtgcttgtcg
     3241 gcaactttct tcattacttc cattgttaac tggttgtggt caaccgcaac gtttgttgta
     3301 tcgaatacag gagccagctc atgctgtgca ggagcaactt cgttatgttt tgttttggca
     3361 ggaataccaa gtttccagag ctctacatcc aggtcatgca tataagctgc gacacgaggt
     3421 ttcagagcac cgaagtaatg atcttccatt tcctggccct taggagcagg agcaccgatc
     3481 agtgtacgtc cgcagaatac aaggtctttt ctctgtttgt ataattcctt gtctaccagg
     3541 aagtattcct gttcaggacc tacagttgta tttacgtgtt tgatatcttt atttcctatt
     3601 atatggagaa ggtttgtagc ttctttatca agtgtctgca tggaacgaag gagaggagtc
     3661 tttttatcaa gagcttctcc gctgtaggag cagaatgctg taggaatgta aagggttccg
     3721 tctttgatga atgccggtga tgtcggatcc catgctgtat atcctcttgc ctcgaatgtg
     3781 gcacgaagac cgcctgacgg gaaactggaa gcatccggct caccttttac aagttcttta
     3841 cctgagaaat ccatgatcac ctgaccatct cctgtaggag aaatgaagct gtcatgtttc
     3901 tctgcagtta cgtttgtcat tggctggaac cagtgagtat aatgagtggc gccgttctcg
     3961 gtagcccatt ctttcattgc aacagcaacg gagttggcaa catcaagttc aaggtgtgta
     4021 ccgctctcga ttgttttctt taatgctttg tacatgtctt ttggcagttt acttctcatt
     4081 actttgtcgt tgaagaccag gctgccgtac agttcaggga tattctgtgc catataggta
     4141 ctctcctttc ggtttgcaat attttctgaa tatgtgtagt gccgctgcag atcaccattg
     4201 tgactgcctg cggttttcgt ctcatagaaa attcctcatc gaacattttc tatgaaacga
     4261 aagttttata aaacgaaaaa agcgccctgg atgagatctc tgtagatctt tccaaagcgc
     4321 ctttgcttta tgtgctgtac tataaacgaa atgaaaacag atgtcaagca ctttttttga
     4381 aaaaattcag aaaaaatttt tgagatatgg caaaaaaagg ataggttgac aaatttgttt
     4441 ctattattat aatttgattt tggaacccgt caaacataag aaaacaatgt cagcgggttt
     4501 tgaaagtaaa gttaaagtca gacaagagaa tacgatcgga ggaaatcaga tggctggaat
     4561 aaaagaagag tgcggcgtct ttggaattta tgatctggat ggcggcaatg tagttccgtc
     4621 catatattat ggactgactt ccctgcagca cagaggacag gaatcctgcg gacttgcagt
     4681 ttctgatact aagggagagc gtggaaatgt gaagtttcat aaggatctgg gacttgtaag
     4741 tgaggttctc agacaggatg ttgtccgtaa atatgaggga gatattggta ttggtcatgt
     4801 aagatattcc acaacaggag cttctgttgc agagaatgca cagccccttg ttttgtctta
     4861 tgtaaaggga actcttgcac ttgcacataa cggaaatctg gttaatacac cggaactgaa
     4921 atgggaactg atccagaatg gagcgatttt ccatacaact acagattcag aggttattgc
     4981 gttccatatc gcaagagaac gtgtgcattc taaaacagta gaagaggcag tgctaaagac
     5041 tgcaaagaag atcaaaggtg catatggtct ggtagttatg agcccgagaa aactgatcgc
     5101 agtccgcgac ccatatggac tgaaaccatt gtgtctcggt aaaagaggaa atgcttatgt
     5161 tattgcttct gagaactgtg ctctgacatc tgtaagtgcg gaatttatcc gtgacattga
     5221 gccgggtgag attcttacga tcacaaaaga cggactgaag tcaaataaag agcttgcgtc
     5281 tgctgctgca aaacgcgc
//
LOCUS       DBMDXG010000105         5269 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3871_length_5269_cov_1.878096, whole genome shotgun sequence.
ACCESSION   DBMDXG010000105 DBMDXG010000000
VERSION     DBMDXG010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_5269_cov_1.878096"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>346)
                     /locus_tag="ACZPD3_04280"
     CDS             complement(<1..>346)
                     /locus_tag="ACZPD3_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HIR1415256.1"
                     /translation="RKGVKMTEPNRQSEENEERIIEIEIERLRPFKEHPFQVKDDKEM
                     FLLQESIEKYGILNPLIVRPVPDGYYEIISGHRRKHAAEKLGYRKVPVIIRVLSEDDS
                     ILSMVDSNLHRER"
     assembly_gap    347..356
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(764..837)
                     /locus_tag="ACZPD3_04285"
     tRNA            complement(764..837)
                     /locus_tag="ACZPD3_04285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Arg,seq:cct)
     gene            complement(1190..2467)
                     /locus_tag="ACZPD3_04290"
     CDS             complement(1190..2467)
                     /locus_tag="ACZPD3_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor C-terminal domain-containing
                     protein"
                     /protein_id="HIR1415257.1"
                     /translation="MTYRELLNLYKQGKLDDVRKKEVEASIEKQDAISEFLYEEGEIP
                     EFSDLESENAENPENEEWLSGQKNNQKDEINRQFIKEIRWSIRKTFIKMGIIVGVAVL
                     VIVLCAVFILPKAVSDFYYNPNEVVGKYEEMTTTRMDLDLSVYSELFLAGNHRSQVSA
                     VSRGYGEYDIIIPQTFSWTGKFTSVSGRLVRGNLTFYDNNILSRPLMQFYLPGDTTAW
                     EAWEVDENGKETKVDTEMRKEESVQDSKDAIAEYNDNDWYLAYVSINDIVDYRDFIEW
                     YQKLSDDKELEWGELWCAVHTEDADGYCVEPNIGFCPYPSGMSMNWDREKYPYLSLLD
                     NTDICHDVDVTDEEIMQTHFISMLSYIQDYDDIISMMGQTTDSPNRYQDMINYVKKNG
                     LKIHGFAVYAKKKNLLELYKQDMVSYVQTILQN"
     gene            complement(2464..3021)
                     /locus_tag="ACZPD3_04295"
     CDS             complement(2464..3021)
                     /locus_tag="ACZPD3_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1415258.1"
                     /translation="MKGVSGAKIKEGGMIEVDNQILLKLYSLYQKELYLYLYSLCGDR
                     HLAEDLLHETFLKALLALPDGHGNMRAWLYMVARNLFYNQQKKKSREILMDEQIYPLE
                     EKTEKGLPEKIFEEEDRRMLYQAIRRLDIKKREIIQMQYFGGMTQKEIAAVLHITLEN
                     VRVLAYRARKELKKYLEECRKEESQ"
     gene            complement(3322..4197)
                     /locus_tag="ACZPD3_04300"
     CDS             complement(3322..4197)
                     /locus_tag="ACZPD3_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3881 family protein"
                     /protein_id="HIR1415259.1"
                     /translation="MHSYLRAVGFSRITKRSSIRQIITDVVETYDEKTVIENYPDGMF
                     AEFSKNYGCDCGITVCGQYDENNVFYPDYYFPFFRGTGITTHESVVIERHADKESFAG
                     ACDDLRIGVTLIFYLQNAAEYLQQREKGNILPGGQSLTLSGLAREGKILFPVEKDKEA
                     VKAERELTKNRNHLIAAARNGDEDAMESLTMEDMDTYSMISQRIVTDDIFSIVDSYFM
                     PYGIECDQYSVMGEILDFMTFKNIITGEEICQLTLDCNDMQFDVCINKNDLLGEPKIG
                     RRFKGIIWLQGQLHY"
     gene            complement(4230..>5269)
                     /gene="serS"
                     /locus_tag="ACZPD3_04305"
     CDS             complement(4230..>5269)
                     /gene="serS"
                     /locus_tag="ACZPD3_04305"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR1415260.1"
                     /translation="RIEELSTREKEVEEELLKDMMVIPNIIDPSVPIGKDDSENVEIE
                     KFGEPVVPDFEVPYHTEIMENFDGIDLDSARRVAGNGFYYLMGDIARLHSAVISYARD
                     FMINRGFTYCVPPFMIRSNVVTGVMSFAEMDAMMYKIEGEDLYLIGTSEHSMIGKFID
                     QIIPEEELPKTLTSYSPCFRKEKGAHGLEERGVYRIHQFEKQEMIVVCRPEESPMWFD
                     KLWQNTVDLFRSLDIPVRTLECCSGDLADLKVKSLDVEAWSPRQKKYFEVGSCSNLGD
                     AQARRLKIRVNGKDGKKYLAHTLNNTVVAPPRMLIAFLENNLNADGSVNIPKALQPYM
                     GGMTKIEKKHA"
BASE COUNT         1437 a         1228 c          854 g         1740 t
ORIGIN      
        1 tccgttctct gtgaagattg gaatctacca tgctcaagat ggaatcatct tcacttaata
       61 cccggatgat taccggtact ttacggtatc ccagtttctc tgcagcatgt tttcttctgt
      121 gaccggatat gatctcatag tatccatccg gtaccggtct gacgatcagc ggatttaata
      181 ttccatactt ctcaatgctt tcctgcagaa gaaacatttc cttatcatct ttcacctgaa
      241 acggatgctc tttgaacggt cgaagacgct caatctcaat ttctatgatt ctttcttcat
      301 tttcttctga ttgtctgttt ggttcagtca tcttgactcc tttcctnnnn nnnnnncagg
      361 attcaggata ctaaaaaatg cctgcctgga acttttatag atagtaaagt tccaaacaga
      421 cattgctgtt tgaccattag ctgtagctaa tagaattgaa tactgtttca gctaattcat
      481 gtttgcaagt ttgggagaga ttgtttcttt tcccgccttt tctctgattt gcatccccaa
      541 ataaggggtg acaacagagc ggaacccaaa tgttagctgg acttcctaaa acccatgttt
      601 gcaaaccggt gattttgatt agctgtcagc taacaaaaat cggattgcgt tagctggaaa
      661 ttgatcattt tgaatggaat tttgtgtcac gaacgtgatt tgaaaataac aaaagcgttc
      721 gttgtcagct aatccagcta acattttcga acgctttgaa gcccgtcgcc aagaggattt
      781 gaacctccga cccctcgctt aggaggcgag tgctctatcc agctgagcta tgacgacaca
      841 tgccgcaaac ccttgatttt actgggtttc tgggattttt cccttcggcc atatattaga
      901 ttatcagctc ttttacaagc tgtcaatcac agatttttct gggttgtttt tttactcaga
      961 gaaatacccc tgctctcttt atagagacgg atactatatc taactgaact atgagaattc
     1021 ctaacccata gctatgattt tactatatct aaagaaaaat tacaagcata atctggcttc
     1081 tcattttaaa ttttacattc agacaaatat tcacaatccg cttcgctgct tacttgattt
     1141 gattaaaaaa gtgcagaaat atctcttctg atatatctgc acttctttct taattttgta
     1201 gtattgtctg gacataggac accatatctt gtttatataa ttcgagaaga ttctttttct
     1261 ttgcataaac tgcaaagcca tggatcttca gcccattttt ctttacatag ttaatcatat
     1321 cctggtatct gttcggagaa tccgtcgtct gtcccatcat tgaaataatg tcgtcataat
     1381 cctgaatgta ggaaagcatg ctgataaaat gggtctgcat aatttcttcg tctgtcacat
     1441 ccacatcatg acagatatct gtattatcca gaagcgacag atacggatat ttctctctgt
     1501 cccaattcat gctcatcccc gacggatacg ggcaaaagcc aatgtttggt tccacacagt
     1561 aaccatccgc gtcttccgta tgaactgcac accagagttc tccccattcc agttctttat
     1621 catcagacag cttctgatac cactcaataa aatctctgta atccacaata tcatttatgg
     1681 aaacataggc aagataccag tcgttatcat tgtattctgc gattgcgtct ttggaatcct
     1741 gaacgctttc ctccttacgc atttctgtgt ctactttagt ttcttttcca ttttcatcca
     1801 cttcccacgc ctcccacgcg gtcgtatctc ccgggagata aaactgcata agtggtctgc
     1861 tcaatatgtt attgtcataa aaagtaaggt ttcctcgcac caaacgtccg ctgacagaag
     1921 tgaattttcc agtccaacta aaagtctgcg gaatgataat atcgtattca ccataccctc
     1981 tcgataccgc actgacctga ctacgatgat tacccgccaa aaacagctca gaataaacag
     2041 aaagatccaa atccattctg gtcgtagtca tttcttcata tttgccaact acttcattgg
     2101 gattataata aaaatctgac acggcctttg ggaggataaa tacagcacat aaaacaatta
     2161 caaggacagc tactcctaca attattccca ttttaataaa agtcttgcgg attgaccacc
     2221 tgatttcttt tataaattgc ctgttgatct catctttttg attgtttttc tgtcctgata
     2281 accattcttc attttccgga ttctctgcat tctcactttc cagatcagaa aattctggta
     2341 tctcaccttc ttcatagaga aattcactga ttgcgtcttg tttttcaata gatgcttcaa
     2401 cttctttttt tctaacatcg tccaattttc cctgtttata taagttcagt aactcacgat
     2461 acgtcattga gattcctcct ttctacactc ttccagatat ttttttaatt ctttccttgc
     2521 ccggtatgca agcacccgta cattttccaa cgtgatatgt aaaactgccg cgatttcttt
     2581 ctgtgtcatt ccgccaaaat actgcatctg aatgatttcc cgcttcttta tatccagtct
     2641 gcggattgcc tgataaagca ttcgcctgtc ttcttcctcg aaaatttttt caggcagacc
     2701 tttctctgtt ttttcttcta aaggatatat ctgttcatcc atcagaatct cccgtgattt
     2761 ctttttctgt tgattataga acaggtttct cgctaccata tagagccatg cccgcatatt
     2821 gccatgtcca tccggcaagg ccagaagtgc tttcaggaag gtttcatgga gcaaatcttc
     2881 tgccagatgt ctgtccccac aaagtgaata caaatataaa tataattctt tctgataaag
     2941 gctgtaaagc tttaataaga tttgattatc cacttctatc attccccctt ccttgatttt
     3001 tgctccggat acacctttca tatctgtttt gtttccagtt ttttatctct ttcactaata
     3061 taacatctat aaacgaaaaa tgttacaaat aaatcaacac atagaaagta catctccctt
     3121 tacttccaga aatgtggtat cttctggatt tccggtgttt tcctgtatat cttcattcca
     3181 tttctgttct gtacgtctta tagcaatcct cgtaaataat gcatttaaga cagttcagca
     3241 gagttcgaag tactatgaca caaaaaaagc atgggcgaag tactctcgtc catgctatca
     3301 ttctaaacat atcttttaat tttaatagtg caactgtccc tggagccata tgattccttt
     3361 gaatctacgt ccgatctttg gctcccccag caaatcattc ttattgatac aaacatcaaa
     3421 ctgcatatca ttacagtcta atgtaagctg gcagatttct tctcctgtta taatgttctt
     3481 gaatgtcata aaatccagaa tctcacccat gacactatat tgatcacatt caatcccata
     3541 tggcatgaaa taggaatcca ctattgaaaa aatatcgtct gtaactatac gctgagaaat
     3601 catggaatat gtatccatat cctccattgt aaggctttcc attgcatctt catcaccgtt
     3661 tctggcggct gcaatgagat gatttctgtt ctttgtcagt tcacgttctg cttttactgc
     3721 ttctttatct ttctctacag gaaaaaggat ttttccttcc ctggccagcc cggaaagggt
     3781 taaagactgg ccacccggaa gtatgttgcc tttttcacgc tgttgcagat actcagcagc
     3841 attctgaaga taaaaaatta aagtaacccc aattctcaga tcgtcacagg cacctgcaaa
     3901 agattctttg tctgcgtgtc tttctatcac aacactctca tgtgttgtaa tgcctgttcc
     3961 gcgaaaaaaa ggaaaataat aatccggata aaatacgttg ttttcatcat actgcccgca
     4021 gacagtaatt ccacaatcgc atccataatt cttggaaaac tctgcaaaca taccatccgg
     4081 atagttctct atgactgttt tctcgtcata ggtttcaacc acatccgtaa tgatctgtct
     4141 gatactgctt cttttggtga tcctggaaaa accaactgcc ctcagataac tgtgcataag
     4201 aacacctccc tgaaaaagct caccctccat taagcgtgct ttttctcaat ctttgtcata
     4261 ccacccatgt atggctgcag agctttcgga atatttacag aaccgtctgc attcaggtta
     4321 ttctccagga atgcgatcaa cattctgggc ggtgctacaa cagtattatt taatgtatgt
     4381 gcaagatatt tcttaccatc ttttccgttt acacggatct taagacgacg tgcctgtgca
     4441 tcaccaaggt ttgaacagct tcctacctcg aaatatttct tctgacgtgg agaccatgct
     4501 tctacgtcaa gagattttac tttcaggtct gccaggtcac cggagcagca ctcaagtgta
     4561 cgaaccggaa tatccagaga acggaacaga tctactgtat tctgccataa tttatcaaac
     4621 cacatcggac tctcttccgg tctgcagaca acgatcattt cctgtttttc aaactgatgg
     4681 atacgataaa cacctctctc ctcaagtcca tgagcacctt tttccttacg gaaacatgga
     4741 gaatagcttg taagagtttt cggaagttct tcctcaggaa tgatctgatc aataaattta
     4801 ccgatcatgg aatgttcact tgttccgatc aggtataaat cttcaccctc aatcttatac
     4861 atcatggcat ccatctcagc aaaactcatt acacctgtta caacattgct tctgatcatg
     4921 aaaggaggta cacagtaagt aaaacctctg tttatcatga aatcacgtgc ataagagatt
     4981 actgcagagt gaagtcttgc gatatctccc attaaatagt agaaaccatt tcccgcaaca
     5041 cgtcttgcac tgtcaagatc gattccatca aagttttcca taatttctgt atggtatggc
     5101 acttcaaaat ccggaacaac cggttcacca aacttctcga tttctacgtt ctcgctgtca
     5161 tctttaccaa tcggtacaga tggatcaatg atattcggaa ttaccatcat atctttgaga
     5221 agttcttcct ctacctcttt ctctcttgtg gaaagttctt caatacggg
//
LOCUS       DBMDXG010000106         5258 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3878_length_5258_cov_1.954625, whole genome shotgun sequence.
ACCESSION   DBMDXG010000106 DBMDXG010000000
VERSION     DBMDXG010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3878_length_5258_cov_1.954625"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            22..5258
                     /locus_tag="ACZPD3_04310"
                     /pseudo
     CDS             22..5258
                     /locus_tag="ACZPD3_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         1846 a          916 c         1299 g         1197 t
ORIGIN      
        1 tatgaaagag aggaccttag catgaagaaa aaaggtaatt ttagaaaagt agtatctggc
       61 ttgctggcag gcatgacaat gcttagtaca gtgctgtctc cgatgacggc atatgcagcg
      121 gagatccggc cagaggaaaa acctccactt tatgaggaag tgaaagacct tctggatgaa
      181 gatgaggtgg tgactgccaa agattatgaa atcgaaacag gcagtgtatt tgatgtgaaa
      241 tcagactaca caggactgga aatcaaggat gataacaagg tcaaagtcac atttgaggaa
      301 gcaaagaaca aaaagaatga ggattttacg acagatcatg cggacactta caaagcggtc
      361 tattatgtgg aaccggtaaa tcaggagcat ccgaagtacc agatcagccg aaagctgatt
      421 gtgcgtgaga aagaaacaga agtgcagaca gaaactgcag ggagtgaagc agtaaccgaa
      481 tccgaaacag ctggcagtga acagcagaca gaagaagcgg aggattcaga atcggactcg
      541 gagattaccg atattgatgc tgatgaattt gatgatttag tcgagcaggc acagaatcag
      601 gatacctatg atgaagaatc agggttagaa cttcatgatg ttttggaaca ggcaggagac
      661 gaaggtgtgg atcttgaatc tatggaagag ggagagattg caacctttga agcagtttcg
      721 gcttattcag caagaagtac acagcaggta acgattgaaa aaggacctct gtatagatat
      781 gcagattaca atcttggaac ctatctgaca gagccgtact atatcagcta cggaagcgtc
      841 agagcaactg cgtattgtgt acagccagca aaacctggac caggatctgg aaattataca
      901 atcacaaaga tcggggacaa ccaggcgtta gctaaagtat gttattacgg cacagatgcc
      961 gcagggagcg aaagcttttt tgcgaataaa cacacggatt tttcagaagg aaaaagattc
     1021 atcattattc acatggctgc atcatatgcc aacggaagca gtgatgcctt ttacggaacc
     1081 aatgctacag gagagacttt ggcaaaagaa ctctataatt attgtgtgaa taagccggaa
     1141 ataccagacg tagcgatgtc attttcaaag ccgaatgtaa aagcttgtgt agatggaaat
     1201 gttcagagaa cggagaacat taagttcaac gcatcttccc agcagaaaat cacaatggac
     1261 ttaccaaagg gagtgaagct ccataatgta tcaactggaa atgtaagtgc ggcaggtgca
     1321 agcgtaacaa ttggaggagg aaccacgttt tatctttcag cacctctgac acagacaaaa
     1381 gatgtaagtg ctactttttc tgcaaaaatg aagggaagta ttacaaaaga ttattcagca
     1441 tataaattga cgacgaatgc aagtgttcag gatctggcat ttgtgtttgg agaaggtgtc
     1501 gctgatgaaa aatatgtaag cttaaaggtg tcctggattg aacaggctac gatcgaaatc
     1561 gtaaagaaag acgatacagc ggatgtcaat cttgcaggtg cagtattcgg tgtatacagc
     1621 gatgaggctt gtacaaagct gatcactcag atgccggcaa cagataagaa tggaaaatct
     1681 tctgttacga tcatcaagac acaggatact gtttatctga aagagatcac agcaccacag
     1741 ggatatgtag taaatgcaac ggcaacaaat gtaaaactgg tagcaagtaa gacctcagct
     1801 gtaaccgtgg aaaataagga acagctggca gagcttacca tttacaaaga aggacaggtt
     1861 ctgaccggag cagaggtcag tgaaaatgga acggtatttc agtatgaaaa ccgcagacag
     1921 aaaaacgcag tatataacgt gtatgccggg gcagatatcg taactgctta tggtacaaaa
     1981 gtgtacagca aaggtgacct tgtaaaagaa aacctgacaa caggtgagaa tggttctgtc
     2041 acactgaaaa acctgcatct tggaacctac gtggtaaaag aaacaaaagc accggactct
     2101 ttctataacg gcagcgaaga aaagtcagtg actctaactt atgctggtca gaacaaagaa
     2161 gttgtatttg cagatgtgac ttttaacaac gagagacaga aagcagatgt ttctgtagta
     2221 aaacaggata aagacaccaa gaaaccactg aaaggcggta tttttgctct gtatgcatcc
     2281 gatgatatta gaaatgcaga cggaaccgtt gttgtgaaaa aaggaacact gattgagaag
     2341 atggaactgc aaaattcaca gcagatcttc cgattggata ttcctattca gtaaaagaag
     2401 atcaggcgcc ggaaggctat gtaagaaata cagaagatgt gtatacgttc aagttttctt
     2461 acaccaatga taaagaagca accgtatcat ttgcccatac cttcagcaat gaccgtgtga
     2521 cagcaaagat caatttattc aaagttgata aagaaacagg aaaagcagta ccgcagggag
     2581 atgccacatt gaaaagtgca gtttatggac tgtatgcccg tgaagatatc gtgcatccgg
     2641 acggagcaac cggaactatt tataaagcag gagaacaggt agcttctctt acaacagatg
     2701 acaaaggaca gtcttctgta aatggcttgt atcttggcaa atattatatc aaagagatta
     2761 caccgccgac cggatacctg gcagatacag aagagcatga tcttacgtgt agttatgaag
     2821 gagatatgac agcagaagta aaaagagagt gcgcttccag tgagcaggtc gtcaaacagc
     2881 cattccagat catcaaagca gccaacaatg gaaagacaga tgcagatctg ttatccggag
     2941 ctggatttac agtttatctg aaatcttccc ttacaaagaa agcagatgga agctatgatt
     3001 ttgattctgc caaaccagta gtaagtggag aaaatggagc aacggagatt ttcacagatg
     3061 aaaaaggata tgcctgcagt attccattac catttggaag ctatattgtg cgtgaaacta
     3121 caacaccgca caattacaag ccagttgatg attttgaagt gaatatcaca gagcatcatc
     3181 caaatgagcc gcagatctgg cgagtgcttc tggataaaga gtttaaggca aaactgaaaa
     3241 tcgtgaaaaa agacgatgaa acaaagaaat ccgtcctgat tgcaggaaca gaatttaagg
     3301 tatatgacct ggataataaa aagtatgttg agcaggtaac aacttaccca gtgacaacaa
     3361 cccataagtc ctattttacg gacaatcagg ggtatctgat catgccgaag aatctgaaga
     3421 tcggacatta ccggattgag gaagtcaacg caccggaagg gtacacaatc aacaagaatt
     3481 atgtagagat tgcagtggat gcaaacacag cataccagat ggattctgaa agcggagatg
     3541 ccattattac ggtagattac gagaaccatc cggtaaaagg aaagctgacg atttataaaa
     3601 aaggtgagat gctgaccgga tttaagaaag attttgttta tgaagaaaga tacctgaagg
     3661 gtgcagaatt taatgtctat gcagctgaaa atatctatac accggattac cagaaagatg
     3721 aaaacggaaa cagacagctg atctatgcaa aagatgcact ggtaacaacg gtaacgactg
     3781 gggaagacgg aaaagcagtt gctgataacc tgccacttgg ttcttactat gtagtagaaa
     3841 agactgcacc ggaaggattt gtcctgaacc atgacagatc agaagtcgca tttgtctatg
     3901 ccaatcagga tacaccggta attgaacagg aagtgactgt tggcgatgac cgacagaaag
     3961 ttgcgattca ggtagaaaaa caggatgcag aaaatggtgc aaccgtagca ggagctgtct
     4021 ttggtatcta taacaaagcg gatattaagg tggatggaaa agtaatcgtg aaagcaaaca
     4081 cactcttaca ggaaatgact tctgataatg atggtcttgc agtatgtacg ctggatctgc
     4141 cacttggaca gtattatgtg aaagagttaa aagcaccggc tggatttgta tcctctgatg
     4201 aagtgctgaa tctggatgct tattatcagg gacaggatgt gaagacagta aaactgaaaa
     4261 ctgtgaaaaa gaaccagcca acaacagttg aaatcacaaa atctgatgta acaacaggtg
     4321 tggaactgga tggggcaaaa ctcaccgttc ttgataaaga aggaaatgtt gtagatcagt
     4381 ggacttctgt aaaaaatcag ccacatgtga tcaaacgact gacagttgga gaggaataca
     4441 ctcttcgtga ggaaatggca ccgtatggat atctgaaagc aacggatgtg aagttcacgc
     4501 tggaagatag agccgagatt cagaaagtag agatgaaaga tgaagttcca acaggacttc
     4561 tgatcatcaa caaaaatgga gaatttctgg ataagataac acttctggat catgtaaaag
     4621 gtaccgtgga gcatctattt gagtatgtaa ccggaagcct ttcagatgtg acgttcgatg
     4681 tatttgctgc ggaagatatc aaagcggcag atggtgtcag tgaagattat ttcaaagcag
     4741 atgaaaaagt gggaaccatt accacagatt ccaatggtat tgcacagatg ggagatctac
     4801 cggctggcaa gtactatgtg aaggaagtaa aaactgccca tggttatgtg ctggacggag
     4861 agccgagata tgttgatctt tcctaccgtg atcaggacac accagtcatt acttatgatg
     4921 agaaatggca gaatgcccgt cagaaagtaa aggttacggt tgtgaagaaa gaaaaagata
     4981 cagaccgtgt ccttgcaggt ggtgtcttcg gtctttatac cagtgaagat atcaaaaatg
     5041 caaagggcga ggtactcctt gagaaagaca gtctgatcga gcagcgagta acggatgaaa
     5101 aaggacagat tacttttacg gcagatcttc cggtagacgg aaaatactat gtgaaagaga
     5161 tctttgcgcc ggacggattt gttacaacag aagaagtaca ggaatttacc tttgaatatg
     5221 ccggagaaga ccaggcagaa gtaagctacg actttact
//
LOCUS       DBMDXG010000107         5173 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3959_length_5173_cov_1.891562, whole genome shotgun sequence.
ACCESSION   DBMDXG010000107 DBMDXG010000000
VERSION     DBMDXG010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3959_length_5173_cov_1.891562"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="ACZPD3_04315"
     CDS             complement(<1..739)
                     /locus_tag="ACZPD3_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415261.1"
                     /translation="MSTTKKSTEQVPFLERPIVRTILPIAGFVIICVLFAILTDGRLF
                     QPKNISLLLSQSYMLLISSIGVFMVMTMGGLDFSQGSMLGVASIVVCYLSHYNMVLAA
                     LGGVVTGGLIGLINGYFNVKRKITSFIVTICTMYLFRGVCAYATTNSPVYAVSDISKY
                     NTLPFMLTFTVIIFVVAYLVFTYTGLGSRLKAIGAGETAARFAGIRVETTKILVFVAA
                     GCITGLAAFVNAIKVGSVTSTAGNQLET"
     gene            complement(752..2248)
                     /locus_tag="ACZPD3_04320"
     CDS             complement(752..2248)
                     /locus_tag="ACZPD3_04320"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR1415262.1"
                     /translation="MGEILLRAENIDKHFGITHALDNVSFTFNKGEIHALIGENGSGK
                     STLTSCLTGIYQKDSGKFILEGKEITATNQVEANHQGVAIIVQEIGTLSGLTVAENIF
                     LGNEDQFTKCGIKNTAAMNKKAQEYLDSYGFNYIDATKVIDDYNFEDRKLVEIVKSTY
                     FNPKVLVVDETTTALGQKGREELFKVMHKVRDTGNCVIFISHDLEEVIEQSDNISVLR
                     DGVKIGSITKEEATPDRLKALMVGREIGDSYYRTDYGEKVSDEVVLSAKNVTVKGQIE
                     NLNLDLHKGEILGIGGLSECGMHEVGKALFGASYFRQGSVTLGDGTPINSIPDAIKHS
                     IAYASKDRDNESLVINDTIGDNICLPSLEDLKTHGFLRAKTMNEFANKFAKQMSTKMT
                     GVDQFVSALSGGNKQKVVLARWVGKDSDLIILDSPTRGIDVKVKADIYAMMDDMRKRG
                     KSIIMISEEIMELLGMADRILIMKDGKINGEFLRSPDLKDTDLIDNMI"
     gene            complement(2260..3198)
                     /locus_tag="ACZPD3_04325"
     CDS             complement(2260..3198)
                     /locus_tag="ACZPD3_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415263.1"
                     /translation="MKKLKQSQYYGIGLLVLMLVVFWAVFKVLAPTTFGSPEKLATYM
                     KSALIYAVGGCGLYFICVMGPFDMSVGANIVLSSIIACNASEKFGYAGLIIAPLICGT
                     IIGLINGIVYIKLHISSLIVTCALSLIYEAFSVYATNGKNVILSADYRAFGDYPVNLI
                     LALIAYLLCAFILKYTKIGTYTYAIGSNEVVAKNMGVNVPKYKIVAFTLAGFFFGLQA
                     ILTISFGTSMTSASNLSSMSRNFTPLMGTFFGLAFKKYGHPVIAIVIGEMIIQLMFNG
                     FVALGAPTTIQNVVTGVALLVIVALTTKPVKGLVVK"
     gene            complement(3357..4499)
                     /locus_tag="ACZPD3_04330"
     CDS             complement(3357..4499)
                     /locus_tag="ACZPD3_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR1415264.1"
                     /translation="MKKRMRKAVAIAATAAMVGSLPAGAVTAQAADDDITIGVSIWSS
                     TDVLGSQSKKIIDKAADALGVNVMYVDQGHVSEQVTASVEQLCAAGCNGIVICNSADS
                     EMTSAIQTCDANQVYLTQFYRIISEENSPEVYQTACNSQYYLGAVHEDEVTNGYTLVN
                     LLLENGDRNIGLEAWTVGDATFQQRWTGYKQAVDEWNEANPDDQATITEPVYANTSSE
                     EGAAAALSLYNSNPDMDALIVAGGGGDPLVGSIGALANEGLTGKIDVVSTDFLDDLGE
                     QLESGGMFAESGGHFCDPLYAFLITYNAIKGNYVKDEGSFGYEIQFPYLYVSSSEDYE
                     NYKKYFVDDDPYTDEELVELAGYSFDDLNKAATSLSIDDVISRHSN"
     gene            complement(4930..>5173)
                     /locus_tag="ACZPD3_04335"
     CDS             complement(4930..>5173)
                     /locus_tag="ACZPD3_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415265.1"
                     /translation="YHMDASYLSRTFSQKYGETIIAFLTRIRMEKAAELMKDQDKKLE
                     TISFLVGYDDYNYFSRVFRKKVGCSPREYRNKFAQL"
BASE COUNT         1400 a         1167 c         1077 g         1529 t
ORIGIN      
        1 gtgtttccag ctggttacct gctgtagatg taacggaacc tactttgata gcatttacaa
       61 atgctgcaag tcctgtgata catcctgctg ccacgaatac gagaatcttt gtagtctcta
      121 cacggatacc tgcgaatctt gctgctgttt cacctgcacc gattgctttc agtctggagc
      181 ccagacctgt gtaagtaaat acaagatatg ccactacgaa gatgatcact gtgaaagtaa
      241 gcataaatgg cagtgtgttg tatttggaaa tatcacttac tgcgtaaaca ggtgagttag
      301 ttgttgcata tgcacaaact ccacggaaca gatacatggt acagattgta acaataaagc
      361 ttgtgatttt tctcttaaca ttgaagtaac cattgatcag tccgatcagt ccgcctgtta
      421 ccacaccacc cagcgctgcc agaaccatgt tgtaatggga aaggtagcag actacgatgg
      481 aagcaacacc aagcatggag ccctgggaga aatccagacc acccattgtc attaccatga
      541 acacgccgat ggatgagatc agcagcatgt atgactggct cagaagcagt gatatatttt
      601 tcggctgaaa cagtctgccg tctgtgagga ttgcaaataa tacacagatg atcacgaatc
      661 ctgcgatagg aaggatcgta cgcacaatgg gacgttccag aaatggtact tgctcagttg
      721 atttttttgt tgtactcatt tttttacccc cttaaatcat attgtcaatc agatctgtgt
      781 ctttcagatc cggagagcga aggaattcac cattgatctt accgtccttc atgatcagga
      841 tacggtctgc catacccaga agctccatga tttcctcaga gatcataata atggattttc
      901 ctctttttct catatcgtcc atcatggcgt agatatcagc tttaactttg acatcaatac
      961 cacgtgtagg ggagtcaagg atgatcaggt cggagtcctt accaacccag cgtgcaagaa
     1021 cgactttctg tttgttaccg ccggaaagag cggatacaaa ctggtcaacg cctgtcattt
     1081 ttgtactcat ctgttttgca aatttattgg caaattcatt cattgtcttt gcacgaagga
     1141 atccatgagt cttcagatcc tccagagatg gaagacagat gttatcgcca attgtatcat
     1201 tgatgaccag agattcattg tctctgtctt tggaagcata ggcaatactg tgtttgatgg
     1261 catccggaat ggaattgatc ggtgtgccgt ctcccagagt tacacttccc tggcggaaat
     1321 aggaagctcc gaagagtgcc tttccaacct catgcatacc gcattcggaa agtcctccga
     1381 taccaaggat ctcacctttg tgaagatcaa ggttcaggtt ctcgatctgc ccttttactg
     1441 tcacgttctt tgcagaaaga acaacttcgt cagatacttt ttcaccgtag tctgtacgat
     1501 agtagctgtc accgatctca cggcctacca ttaaggcttt cagtctgtca ggtgtagctt
     1561 cttcctttgt gatggaaccg atcttcacac catctcgaag tacactgata ttatcagact
     1621 gctcgataac ctcctcaagg tcatgggaaa taaagattac gcagtttcct gtgtcacgaa
     1681 ccttgtgcat taccttgaaa agctcttcac ggcctttctg tccaagtgct gttgttgtct
     1741 cgtctacaac cagaaccttc ggattaaagt aggtagattt tacgatctct accagcttac
     1801 gatcctcgaa gttgtaatca tcgatcacct tagttgcatc aatatagttg aatccgtaac
     1861 tgtccagata ttcctgggct ttcttgttca ttgctgcggt atttttgata ccacatttag
     1921 taaactgatc ctcgtttcca aggaaaatat tctctgctac agtcagtccg gaaagtgttc
     1981 cgatctcctg tacgataatg gctacaccct gatggttggc ttcaacctgg ttggttgcgg
     2041 tgatctcttt tccttcaagg ataaacttgc cggaatcttt ctggtaaata cctgtcaggc
     2101 aggatgttaa tgtagattta ccggaaccgt tttcaccgat cagtgcgtgg atctcacctt
     2161 tattaaaggt aaaggaaacg ttgtccaggg cgtgtgtgat gccaaaatgt ttatcaatat
     2221 tttctgctct taataatatt tcacccataa tcgttctcct tattttacta caagaccttt
     2281 aaccggttta gttgtcagtg caacgataac cagcagagca acacctgtta ctacgttctg
     2341 gatcgtagtc ggagctccca gtgcaacgaa accgttgaac ataagctgaa tgatcatttc
     2401 accgattaca atagcgataa ccggatggcc atatttctta aatgccagtc caaagaatgt
     2461 tcccatcaga ggagtaaagt tacggctcat agaggaaagg ttggaagctg atgtcatgga
     2521 agtaccaaaa ctgattgtca ggattgcctg aagtccgaag aaaaatcctg caagagtaaa
     2581 tgctacgatc ttatatttcg gaacatttac acccatgttc ttggctacaa cttcgttact
     2641 tccgatggcg taagtataag taccgatttt tgtatatttc aaaatgaatg cgcaaagcag
     2701 atatgcgatc agtgccagga tcaggttgac cggataatca ccaaatgctc tgtagtctgc
     2761 tgaaagtatt acgttctttc cgtttgtggc gtatacactg aaggcctcat agatcaggga
     2821 cagcgcacat gtaacgatca gggaagagat atgtaattta atataaacaa taccattgat
     2881 gagaccaatg atcgtaccac agatcagcgg tgcaatgata agaccggcat aaccgaattt
     2941 ttcacttgca ttgcaggcga taatggatga aagtacgata ttggcaccaa cgctcatatc
     3001 gaaaggtccc attacgcaga taaagtagag gccacatccg ccaactgcgt aaattaatgc
     3061 tgatttcatg taagtggcca gtttctccgg ggaaccgaaa gtagtcggtg caagtacttt
     3121 aaatactgcc cagaaaacta caagcataag aaccagaagg ccgattccat aatactggct
     3181 ttgtttgagt tttttcataa ccattctcct tatccaattc atcgcccgcc aaaagcagga
     3241 ggtcacacgc ttcatcaggg tttggccttt tgcttcgagc gggcactgac aattacctct
     3301 taaaagtttc tccactgccc tttggagaaa aggcagtgga gcacaagttc atcatattag
     3361 ttgctgtgac gggaaataac atcatcaata gaaagtgatg tagctgcttt gttcagatca
     3421 tcaaagctgt atcctgctaa ttccacaagc tcttcatctg tgtatggatc gtcatctaca
     3481 aagtatttct tgtagttttc atagtcttcg gaagaagata catacaggta tgggaactgg
     3541 atttcgtatc cgaaggatcc ttcatctttt acatagttgc ctttgattgc gttatatgtg
     3601 ataaggaatg catataacgg gtcgcagaag tgtccgcctg attcagcaaa cattccgcct
     3661 gattcaagct gctcgccaag gtcatcaagg aagtctgtgg aaacaacgtc gatctttcct
     3721 gtaagtcctt cgtttgccag agcaccgatg gaaccaacaa gcgggtcacc gccaccacct
     3781 gcaacgatca gagcgtccat atcagggttg gaattgtaaa gggaaagagc tgctgctgca
     3841 ccttcttcag aagatgtgtt agcatataca ggctctgtga ttgttgcctg atcatcagga
     3901 ttagcttcgt tccattcatc aactgcctgt ttgtaacctg tccatctctg ctggaatgta
     3961 gcatcaccaa ctgtccatgc ctcaaggccg atgtttctgt caccgttctc aagaagcagg
     4021 ttaaccagtg tatatccgtt tgttacttcg tcttcatgaa ctgcaccaag ataatactgt
     4081 gagttgcatg ctgtctgata tacttcaggg ctgttctctt cggagatgat acgatagaac
     4141 tgtgtaaggt aaacctgatt tgcatcacat gtctggatgg cagatgtcat ttcggagtct
     4201 gcagagttac agattacgat accgttacat cctgctgcgc aaagctgttc aacagaagct
     4261 gtaacctgct cagatacgtg gccctggtca acatacatta cattaacacc aagagcatct
     4321 gctgctttgt caatgatttt tttactctgg cttccaagta cgtctgtgga gctccagatt
     4381 gatacgccga ttgtgatgtc gtcatcggct gcctgtgctg taactgcacc agccggtaaa
     4441 gaaccaacca ttgctgctgt tgctgcgata gcaactgctt ttctcattct ttttttcatg
     4501 ttgtaaaacc ctccttgaaa aattttggtt tcttattttt ttgaacggaa gtttttccgg
     4561 gctttccgtt cctctgtgat tatattaaac catactttgc ctttggtcag ttaggacata
     4621 tttggaaaat tttgttgcac atttttgacc agtttatata taaaacttgg gcaaaatatg
     4681 aacaagttgg gcaaaatgcc agtaacttgt atgttttctc tattgattat agcaattcca
     4741 taaaataatg caatcaagac agttcagcag agtgcgaagg acaaggaaga tacctgcagg
     4801 cgtgctgacg cacggcaaag atatcttccc aaaaagtcct ctgtatccat tcgattcgat
     4861 aagaaccgcc atacagagga cttccgcttt cgttactgtc cactcaattc tacctgagtt
     4921 tcttccatat tataattgtg caaacttatt ccgatactcc ctcgggctgc atcccacctt
     4981 ctttcggaac actctgctga agtagttata atcatcatat cccaccagaa acgaaatcgt
     5041 ctccagcttc ttgtcctgat ctttcataag ctctgccgct ttctccatcc ggattctggt
     5101 aagaaatgca atgatcgtct caccgtattt ctgactgaaa gtcctggaga gatagcttgc
     5161 atccatatga tac
//
LOCUS       DBMDXG010000108         5171 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3962_length_5171_cov_1.566864, whole genome shotgun sequence.
ACCESSION   DBMDXG010000108 DBMDXG010000000
VERSION     DBMDXG010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3962_length_5171_cov_1.566864"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="ACZPD3_04340"
     CDS             complement(<1..553)
                     /locus_tag="ACZPD3_04340"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1415266.1"
                     /translation="MTKIRTRFAPSPTGRMHVGNLRTALYAYLITKHEGGDFILRIED
                     TDQERYVEGAVDIIYRTLAKTGLLHDEGPDKDKGFGPYVQSERQAQGIYLKYAQQLVE
                     QGDAYYCFCKKEELEGMKRVVAGKEISIYDKRCLKLSKEEVQRRLDAGEPHVIRFNMP
                     TEGTTTFHDVIYGDITVNNEELED"
     gene            complement(1310..3025)
                     /locus_tag="ACZPD3_04345"
     CDS             complement(1310..3025)
                     /locus_tag="ACZPD3_04345"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR1415267.1"
                     /translation="MADLKRFFKKTVSEDRHKQAASVSVPEGLWIKCPKCGEMVYRED
                     VISNAYVCPKCGGYFRISAKRRIKMIADKGSFKEWFTGIDNSNPLDYPDYPQKIEDLR
                     EKTHLDEAILIGEATIGGIRTVIGAMDTRFLMASMGYVVGEKITRSFEEATKQGLPVI
                     LFCCSGGARMQEGIVSLMQMAKTSAAIKRHSQAGLLYTSVLTDPTTGGVTASFAMLGD
                     IILAEPGSLIGFAGPRVIEQTIGQKLPEGFQRPEFLLEHGFLDGIVERGSMRDVLSLI
                     LKLHDTRKCYGEFPQETSARSFDTFGKKKKQKKQKNLTAWDRVQIARSSDRPSVAEYI
                     DAIFDDFMEFHGDRGVRDDNAIIGGIATLDGQPVTVIGIRKGHTTKENIRCNFGMPSP
                     EGYKKALRLMKQAEKFGRAVIAFVDTPGAFCGLEAEERGQGEAIARNLLEMSDLKVPV
                     LSVVIGEGGSGGALALAVGNEVWMMENATYTILSPEGFASILWKDSRKAPEAAEVMKV
                     TAAHLKELGIIERIIPEEYPASEENLPEIAEYMKIRMKQFLEKQSGKSGEQIAQERYE
                     RFRKM"
     gene            complement(3208..4554)
                     /locus_tag="ACZPD3_04350"
     CDS             complement(3208..4554)
                     /locus_tag="ACZPD3_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR1415268.1"
                     /translation="MFNKILIANRGEIAVRIIRACREMGIRTVAVYSEADRDALHTQL
                     ADEAVCIGKAQSSDSYLNMERILSATIATKAEAIHPGFGFLSENSRFVEMCEKCNVAF
                     IGPSAEVISRMGNKSEAKNTMRKAKVPVVPGTKEPVYTVEQALEAVKEIGFPVMIKAS
                     AGGGGKGMRVAGNEQEFAKLFETAQQESVHSFSDNTMYLERFVENPRHVEVQILADKY
                     GNVVHLGERDCSVQRRHQKMIEESPCIAISDDLRKKMGETAVRAAKAAGYESAGTIEF
                     LLDQSGEFYFMEMNTRIQVEHPVTEFVSGVDLIKEQICIAAGEPLSVQQKDIEIRGHA
                     MECRINAEDPERHFMPCPGKITDLHLPGGNGIRVDTAVYNDYAIPPYYDSMIAKIIVY
                     DKDRRSAIRKMISALGEVAIEGVKTNVDFLYELLNQPDFQEGNITTDFIPQHYPDL"
     gene            complement(4558..4989)
                     /gene="fabZ"
                     /locus_tag="ACZPD3_04355"
     CDS             complement(4558..4989)
                     /gene="fabZ"
                     /locus_tag="ACZPD3_04355"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR1415269.1"
                     /translation="MKLTTKEIMEIIPHRHPFLLIDTIEELVPGVKATGKKCVTYNEP
                     HFAGHFPQEPVMPGVLIVEALAQTGAVAILSKPENKGKIAYFASINNAKFKNKVVPGD
                     TLTLEVEIIKEKGPMGVGKAKATNQDGKVAVIAELTFAVGA"
     gene            complement(5009..>5171)
                     /locus_tag="ACZPD3_04360"
     CDS             complement(5009..>5171)
                     /locus_tag="ACZPD3_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR1415270.1"
                     /translation="KVGDKVSKGQVLGIVEAMKLMNEIESEFDGTVKEILVENEQMVE
                     FGQPMFVIE"
BASE COUNT         1250 a         1289 c          996 g         1636 t
ORIGIN      
        1 gatcttccag ttcctcattg tttactgtga tatctccgta gattacatca tggaatgttg
       61 tagttccctc tgttggcata ttgaaacgga ttacgtgtgg ttctcctgca tcaagacgac
      121 gctgtacttc ttctttggaa agtttaaggc agcgtttgtc ataaatggaa atctcttttc
      181 ctgccacaac acgtttcata ccttccagtt cttccttctt acagaaacag tagtatgcat
      241 caccctgttc tactaactgc tgtgcatatt tcagatagat tccctgtgcc tgacgctcac
      301 tctgaacata cggtccaaag cctttgtcct tgtccggacc ttcatcatgg agaagtcctg
      361 tttttgcaag agtacggtag ataatatcta cagcgccttc tacgtaacgt tcctggtctg
      421 tatcctcaat acggaggata aaatctccgc cttcatgctt tgtaataaga tatgcgtata
      481 acgcagtacg taaatttcca acatgcattc tgcctgtagg gcttggtgca aatcttgttc
      541 tgatcttggt cataactttt ttcactcctg aattttttat atattcaaaa tctgtaaaaa
      601 aagaccctgc ttaaaagcag cgtctttcta ctttcgggtt tattatacgt cagaggcttt
      661 cttatttcaa gtcgggatga gggaaaattt taatgttttt tgggaaggga cgcatgggaa
      721 gcaattatgt ttgggggctc agtttcggaa ccattgtatt tctaggcatt cgggaagccg
      781 ctaaaatcat gagccgctct ctcaaactcg ctccgttgca aactccgctc agacagtgaa
      841 acgatcggct cataacgcgg cttcccaaat accaagaact acaatgaacc ctcaaattcg
      901 cccccaaaca taattgcttc ccatgctgac tatcggctat ataacatatt tagtgtacaa
      961 caataaagaa tataccactc ctgactttac agaacaatta gccaaataac atatctgcta
     1021 cacaacataa ctatagtaac cctaagaaag tgcatgacaa cttgcaacaa aacagcgcag
     1081 aaaaatattt tatttggagt gcatttgcag gggtcttaga tattcttagt tctcagaaat
     1141 ccgcgttatg agccggctgc atcactgtct gagcgaagtt tgcaacggag cgagtttgac
     1201 aagacggctc atgcctttag cagatttctg agagcttaga atatcttaga ccccgaaacc
     1261 gcactccaaa taaaatattt ttctgcgcgt ccctcttgcc attcacacgc tacatcttac
     1321 gaaatctctc atacctttcc tgcgcgatct gttctccact tttaccactc tgtttctcca
     1381 gaaactgctt cattctaatc ttcatatact ctgcaatttc cggcaaattc tcctcacttg
     1441 caggatattc ctccggtatg atccgttcaa tgattcccag ttctttcaga tgagccgccg
     1501 ttactttcat gacctctgca gcttccggtg cctttctgct gtctttccag agaatggaag
     1561 cgaaaccttc aggtgaaagg atggtgtagg ttgcattttc catcatccag acttcattgc
     1621 cgactgcaag tgcaagtgca ccgccactgc cgccttcacc aattacaacc gaaagaaccg
     1681 gcaccttcaa atcggacatt tccagaagat tccttgcaat ggcttcgccc tgtccccgtt
     1741 cttctgcttc cagtccacag aatgcccccg gagtgtctac aaatgcaatg actgcacgtc
     1801 cgaatttctc tgcctgtttc atcagtcgaa gagccttttt gtacccttcc ggtgatggca
     1861 ttccgaagtt acatctgatg ttttcttttg tagtatgccc tttacggatt ccgattactg
     1921 taacgggctg tccgtccagt gtagcaatgc cgccgatgat ggcattgtcg tctctgacac
     1981 ctctgtcacc atgaaattcc atgaaatcat caaaaatggc atcaatatat tccgctactg
     2041 acggacggtc actgcttctg gcaatctgga ctctgtccca ggctgtgaga tttttttgtt
     2101 tcttctgttt tttctttttg ccaaaagtat cgaaagatct ggcagatgtt tcctgtggaa
     2161 attctccgta acattttctg gtatcatgca gttttagaat cagcgaaaga acatcccgca
     2221 tagagcctcg ttcaacaatt ccgtcgagaa atccatgttc cagaagaaat tccggtctct
     2281 ggaagccctc cggcagtttc tgacctatag tctgttcaat gactctcggg ccggcaaatc
     2341 cgatcagtga gcctggttct gcaaggataa tatctccaag catggcaaag cttgctgtaa
     2401 cgccaccggt agtcgggtct gtcaacacag atgtgtagag aagccctgcc tgggagtgac
     2461 gtttaatggc tgcggaggtt tttgccatct gcatcagtga tacgattcct tcctgcattc
     2521 tggcaccgcc ggagcaacag aacagaatga ccggaagtcc ctgttttgtt gcttcttcaa
     2581 atgagcgggt gatcttttct cccaccacat atcccatgga agccatgaga aatctggtat
     2641 ccatggctcc gatcactgta cggattcctc cgatggtggc ttctccgatg agaattgcct
     2701 catcaaggtg tgttttttct ctcagatcct caattttctg cggataatcc ggatagtcca
     2761 gtggattgct gttgtcaatt cctgtgaacc actctttaaa acttccttta tctgcgatca
     2821 ttttgatccg gcgtttcgca ctgattctga aatatcctcc gcatttcggg cacacatagg
     2881 cgttggatat tacgtcttct ctgtaaacca tttctccgca ctttggacat ttgatccata
     2941 agccttcagg tacggaaaca gatgcggcct gtttatgccg gtcttctgaa actgttttct
     3001 taaaaaatct cttcaagtct gccattaaaa tcctgctcct ttatgtaacc caatttgatg
     3061 ttctttcaga caacaaaata acgttaataa ctattaatca aactaattct gtcaaaaatt
     3121 attttatcga tttgcttatc aattaccttt caataataag tatgtaattt ctgctgaact
     3181 ataaaatata tttgcaccag ttttctttta cagatccgga taatgctgcg gaatgaaatc
     3241 tgtggtgata tttccttcct gaaaatccgg ctgattgaga agttcataca gaaaatccac
     3301 gttagttttt actccttcga tggcaacttc tccaagtgca ctgatcatct ttcgaatggc
     3361 actgcggcgg tctttgtcat aaacaatgat cttggcaatc atagaatcat agtagggcgg
     3421 gattgcgtag tcattataaa ctgctgtatc cacgcggatt ccgtttccac cgggaagatg
     3481 taagtcagtg atcttaccag ggcatggcat gaaatgtctc tcaggatcct cggcattgat
     3541 tctgcattcc atggcgtgac cgcggatttc tatgtctttc tgctgaacgc ttaacggttc
     3601 acctgccgca atgcagatct gctctttgat caggtctaca ccagacacaa attctgttac
     3661 cggatgttct acctggattc tggtgttcat ttccatgaaa taaaattctc cagactgatc
     3721 cagaagaaat tctatggttc ctgcactttc atagcctgct gcctttgctg cccgtactgc
     3781 tgtctcaccc attttctttc tcagatcatc agagattgcg atacatggag actcttcgat
     3841 cattttctgg tgacgtctct gaacagagca gtctcgttct cccagatgaa ctacatttcc
     3901 gtatttatcc gcaagaatct gcacttcaac atgtctggga ttctccacaa accgttccag
     3961 atacatggta ttatcggaga aggaatggac cgactcctgc tgtgctgtct caaacaattt
     4021 ggcaaattcc tgttcatttc cggctacacg cattcctttt ccaccgccgc ctgctgatgc
     4081 tttgatcatg accggaaaac ctatttcctt tacagcttcc aaagcctgct ctactgtgta
     4141 gaccggttcc tttgtgccgg gcactaccgg gactttagct ttccgcattg tgtttttcgc
     4201 ctcagattta tttcccattc tggaaattac ttctgcggaa ggaccaataa atgccacatt
     4261 acatttctca cacatttcca caaaacggct gttctctgag agaaatccga atcccgggtg
     4321 gattgcttct gcttttgtgg caatggtagc acttaagatt cgctccatgt tcagatagct
     4381 gtctgatgac tgtgcctttc cgatgcatac tgcttcatct gcaagctggg tatgcagggc
     4441 atctctgtct gcttctgagt atacggctac tgtgcggatt cccatctcac gacatgcccg
     4501 gatgatcctc actgcaatct caccacgatt ggcgatcaaa attttattaa acatctgtta
     4561 tgctcctact gcaaaagtca gctctgcgat cactgctacc ttaccgtcct ggtttgttgc
     4621 ctttgctttt cccactccca tgggaccttt ttccttaatg atctctactt cgagagtaag
     4681 ggtatctccg ggaactacct tgttcttgaa ttttgcattg ttgattgatg caaaatatgc
     4741 gatctttcct ttattttccg gtttactcag aattgcaact gcacctgtct gcgcaagcgc
     4801 ttcaacgatc agaactcccg gcataaccgg ttcctgtggg aagtgtcctg cgaaatgcgg
     4861 ctcattatag gtaacacatt tctttcctgt tgccttcaca cccggcacca gttcttcaat
     4921 agtatcaatc aggaggaacg ggtgtctgtg gggaatgatc tccatgattt cttttgttgt
     4981 aagtttcata gttttttacc tccttcttct attctatcac aaacatcggc tgtccgaact
     5041 ctaccatctg ctcgttttct acaaggattt cttttactgt accatcaaat tctgattcaa
     5101 tctcgttcat cagcttcatt gcttccacaa tgccaaggac ctgacctttg cttactttat
     5161 ctcctacttt t
//
LOCUS       DBMDXG010000109         5137 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_3990_length_5137_cov_2.823272, whole genome shotgun sequence.
ACCESSION   DBMDXG010000109 DBMDXG010000000
VERSION     DBMDXG010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3990_length_5137_cov_2.823272"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="ACZPD3_04365"
     CDS             <1..1542
                     /locus_tag="ACZPD3_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR1415271.1"
                     /translation="KKSVGDLARSMSLNIKKVWLKREEQEILVPASDILPGDRVIVHM
                     GNVIPFDGEVSCGEGMVNQASLTGESLPVRRTEGQSVYAGTVLEEGELEIIVRAVSGA
                     TRFEKIVTMIEDSEKLKSNLESKAEHLADRLVPYTLLGTGLVWAVTRNMTKALSVLMV
                     DFSCALKLAMPISVLSAIREAGQNGITVKGGKYLEAVAEADTIVFDKTGTLTKAKPTV
                     KEVIVFGDYPEPEALRIAACLEEHFPHSMAKAVVDAAKRRSLYHEEMHSKVEYIVAHG
                     ISSYIEGKKTIIGSSHFVFEDEKCRIRPEYQERFDTLPEEYSHLFLAIDGELVAVICI
                     EDPLREEAGEMVRFLKEEGVSKIVMMTGDSERTAASIAKRVGVDEYYSEVLPEDKAGF
                     IEREKAAGRKVVMIGDGINDSPALSAADAGIAISDGAELAREIADITIAAEDLREIVV
                     LKRLSNAMMHRIQGNYRGIVGINAMLIALGVAGIIQPTTSALLHNTSTLAISLKSMGN
                     LLPTN"
     gene            complement(1602..3923)
                     /locus_tag="ACZPD3_04370"
     CDS             complement(1602..3923)
                     /locus_tag="ACZPD3_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1415272.1"
                     /translation="MERCDFGSIMTIIRRYISEDKGMNQIDFTYLLFDTFMCSDEAID
                     FDFDNGQVCRWMTGQAKVSPRIVTYFLDKEHQLELAGNIQRHIIPLMYDSAMAAKELY
                     ELVLQDTSISEPKKQELICSYSPADIDTIHIFITRVLCFGMERNFVKRDTRTKKLLAA
                     GNLSPVLTDFVMGNDVPRPCRHFCGRSEEIEVLHSLLEKERKVFLSGIAGIGKSELAK
                     AYALQYKKEYTNILYLTYSGDLKQDITDMDFADDLPQDSDEERFRKHNRFLRSLKEDT
                     LMIVDNFNATSIQDSFLSVILKYRCQILFTTRSRLDGHSCMLLEEISDKTTLLQLAGK
                     FFSDTEEKSDVIEQIIEAVHAHTLAVELASRLLETGILEPEMLLKKLLEENVALDATD
                     KINIIKDGQSSKETYYGHIHTLFSLYQLSETQQDVMRCLCLIPLTGIPARRFAAWLNL
                     PDLNAVNDLIEMGFIQPKTGRTIVLHPMIQEIAVADMQPSVKTCFPLLESLQNICLLH
                     GNDISYYRLVFQTVENIITKTTKDDISGYLLFLEDVFPYMEKYHEENGMQRILRELSS
                     LLEDTSIGTVSDRALLLDYKATLERNIGKAVKLEKEAMSLLSPVTPENAHLVANLYGN
                     LGGLYHQQGNTELAKQAMEQGISLLEQYQLLYMNDSIVQICNYAALLTDTGEASRGLS
                     SLRKCARLVKEYNSDQYLDYAIIQEAMGTAYLVQADIEQATSHLKKAMAIYEIVWESE
                     PEAIDNKYQQIQELYINAGIQIGQQLLSSTKNV"
     gene            4124..4288
                     /locus_tag="ACZPD3_04375"
     CDS             4124..4288
                     /locus_tag="ACZPD3_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415273.1"
                     /translation="MAHTVEFERCVDFLARMIEKYGSELLQELNGLPKEDKAEDNCQQ
                     EQQQNTLGND"
     gene            4427..>5137
                     /locus_tag="ACZPD3_04380"
     CDS             4427..>5137
                     /locus_tag="ACZPD3_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1415274.1"
                     /translation="MKKRRCYIYTRVSTSMQVEGYSLEAQKERLRKYADFQEMVIVGE
                     YSDEGKSGKSIEGRQEFMQMLRDIEEGKDRVEFVLVYKLSRFGRNAADVLNSLQRMQD
                     FGVNLICVEDGIDSSKDSGKLMISVLSAVAEIERENILVQTMEGRRQKARDGKWNGGF
                     APYGYKLLNGELEIAEDEAEIIRIIFDKYIHTNMGVNGIAAYLNEHGYKKKKRQNNTL
                     DAFATSFIKGVLDNPIYCG"
BASE COUNT         1535 a          896 c         1197 g         1509 t
ORIGIN      
        1 aaaaagtctg taggagatct ggcacgcagc atgtctttaa acataaagaa agtatggctg
       61 aaaagagagg agcaggaaat tcttgttccg gcatccgata ttttgccggg tgacagagtg
      121 atcgtacaca tgggcaatgt aatcccattt gatggagagg tgtcttgcgg agaaggaatg
      181 gtgaaccagg catctttgac cggagaatct cttccggtac gaagaactga gggtcagtcc
      241 gtatatgcag gaaccgtttt ggaggaagga gagttggaaa tcatagtcag ggccgtatca
      301 ggtgctaccc ggtttgaaaa gattgtgact atgattgaag actcagaaaa gttgaaatcc
      361 aatctggaaa gcaaggcaga gcatctggcg gaccgtctgg taccatatac gcttctgggg
      421 acagggcttg tctgggctgt cacacgcaat atgacaaaag ctctttctgt tcttatggtg
      481 gatttttcct gcgcattgaa gctggctatg ccgatttctg tcctttctgc aatacgggaa
      541 gccggacaga atggaattac cgtaaagggt gggaagtatc tggaggctgt ggcagaggca
      601 gataccatcg tatttgataa gaccggtacg ctgacaaagg cgaaaccaac tgtaaaagaa
      661 gtcattgtgt ttggcgatta tcctgaaccg gaagctctgc ggattgctgc ctgtctggaa
      721 gaacactttc cacattccat ggcaaaagct gtcgtggatg ctgcaaagag aagaagtctg
      781 tatcatgaag aaatgcattc aaaagtggag tatattgtag cccatggtat ttcctcctat
      841 atagaaggaa agaagaccat aatagggagc agtcattttg tgtttgagga tgaaaagtgc
      901 cggatacgtc cggaatatca ggaacgattt gatacgcttc ctgaagaata ctctcatttg
      961 ttccttgcca ttgacggaga attagttgct gtaatctgta ttgaagatcc tctcagggaa
     1021 gaagccgggg agatggtaag atttttgaaa gaagaaggtg tttccaagat cgtcatgatg
     1081 acgggagaca gtgaacgtac tgcagcatct attgcgaaac gagtgggagt ggatgaatat
     1141 tattccgaag tacttccgga agataaggca ggattcattg aaagagagaa agcggcagga
     1201 agaaaagtag tgatgatcgg tgacggaatc aatgattcgc cagcgctttc agcagcagat
     1261 gcgggaattg caatcagtga tggagcagaa cttgcgcgag agatcgcaga cattacgatt
     1321 gctgctgagg atctgcggga aatcgtagta ctgaaacgtt tgtctaatgc gatgatgcac
     1381 cgtattcagg ggaattacag ggggattgtc ggtattaatg caatgctgat tgcactagga
     1441 gtggcaggaa ttattcaacc aacgacctct gcgttactac ataatacttc tactttagct
     1501 atcagtttga agagtatggg aaatttattg ccgactaatt aatataaaat attcatattg
     1561 ttataagttt taatgtctga atagtttgat ttgtagacta ttcagacatt tttagtagag
     1621 ctcaataatt gttgtccaat ttgtattcca gcattgatat acaattcttg tatttgttgg
     1681 tatttattat cgatagcttc cggttcagat tcccatacta tttcatagat tgccatagct
     1741 tttttgagat gggaggtagc ctgttctata tcagcctgta ccagataggc agttcccata
     1801 gcttcctgaa taatggcata gtccagatac tgatcggaat tgtattcttt taccagccgg
     1861 gcacatttgc gtaaggagga taatccacga gacgcttcac cggtatctgt aaggagagct
     1921 gcatagttgc agatttgaac aatactgtca ttcatataga gaagctggta ttgttctaat
     1981 aagctgattc cctgctccat agcctgtttt gccagttcgg tatttccctg ctggtgatat
     2041 agtccaccca gatttccata tagatttgcg acgaggtggg cgttttcagg tgtaacagga
     2101 gaaagcaaag acatcgcttc tttttccagc tttacggctt ttccaatatt tctttcaagg
     2161 gtagctttat agtccagtaa taatgcccgg tcagacacgg ttccgataga agtgtcttcc
     2221 agcaaagaag atagttcacg caggattctt tgcataccat tttcttcgtg gtatttttcc
     2281 atatatggga aaacatcttc taagaataat aaataaccag aaatatcatc tttagttgtt
     2341 tttgtaataa tgttttcaac ggtttggaaa acaagtctgt agtaggaaat atcattgccg
     2401 tgtaaaaggc agatgttttg cagactttcc agtaaaggga aacaggtttt tacagacggc
     2461 tgcatatctg caacagcgat ttcctgtatc atgggatgca gtacaatggt tcttccggtt
     2521 tttggctgaa taaatcccat ttcaattaaa tcatttacgg cattcaaatc cggtaaattc
     2581 agccatgcag caaatcttcg tgccggaata ccggttaaag gaatcaggca cagacatctc
     2641 attacatctt gttgagtttc agaaagctga tatagagaga ataaagtatg tatgtgtcca
     2701 tagtaggttt ctttgctgct ttgcccgtct tttataatat tgattttatc agttgcatca
     2761 agagcgacat tttcttccaa caattttttt aagagcattt ctggttccaa tattccggtt
     2821 tccagtaagc gtgatgccag ttctacagcg agcgtatggg catggacagc ttctatgatc
     2881 tgttctatga catcagattt ttcttctgtg tcggagaaaa attttcctgc aagttgcaga
     2941 agcgttgttt tatcggaaat ttcttccaac agcatacagg aatgtccgtc cagacggctt
     3001 ctggtagtaa ataatatctg acagcggtat ttgagtatga ccgaaagaaa actgtcctgt
     3061 atggaagtgg cgttgaaatt atctacaatc atcaaagtat cttctttgag agaacgcaga
     3121 aaacggttgt gttttcggaa gcgttcttca tcagaatcct gcggaagatc atccgcaaaa
     3181 tccatgtctg taatatcctg ttttaaatca ccgctgtagg tgagatacag gatattggta
     3241 tattctttct tatactgcag ggcataagcc tttgccagtt cacttttccc gattccggca
     3301 attccagata aaaatacttt acgctctttt tccaacagag aatgcagcac ttcgatttct
     3361 tcagaacgtc cacaaaaatg cctgcatgga cggggaacgt catttcccat tacaaaatct
     3421 gttaagacag gagaaagatt tccggcggct aataatttct tggttcgtgt gtcacgtttc
     3481 acaaaattcc gttccattcc gaaacacaga acccttgtta tgaaaatatg tatggtgtct
     3541 atatcagcgg gagaataaga acagattagt tcctgttttt ttggctctga aatagatgta
     3601 tcctgtaata ctaactcata taattctttt gctgccatag cagaatcata catcagagga
     3661 ataatatgtc gctgaatgtt tcctgccaat tctaattgat gttctttatc taaaaagtag
     3721 gttacgatcc ggggacttac ttttgcctgt cctgtcatcc aacggcagac ctgtccattg
     3781 tcaaaatcaa agtctatggc ttcatcgctg cacataaacg tgtcaaataa cagataggta
     3841 aaatctattt gattcattcc tttatcttcg ctgatataac ggcgaatgat cgtcataatc
     3901 gaaccaaaat cacatctttc caaatagaat cccctcctgt tctgtaaaat actcaatttc
     3961 cgctcaatct atgctcaatg agtttcttat gaaattccat taaactgaca tcaggctgaa
     4021 cggagttggt ctaatagaaa ttatagcaca aattccagat cagctaaaga gtaccgccga
     4081 aaaatgcgtg gtgacaagaa atatcggttt ggaaggagga gctatggcac acacagttga
     4141 atttgaacga tgtgttgatt ttcttgccag aatgatagaa aaatatggca gtgagctcct
     4201 tcaggaactg aatggattac cgaaggagga caaagcagaa gataactgcc agcaagagca
     4261 gcaacagaat acattgggaa atgattaggg agagcataga tgttggctct cttttttctt
     4321 gcgtgatatt tgtgcatttt acctatattg acgagtatgt gcagctacgg taaaatatag
     4381 ttgggacaaa taaaaagtaa actgtaattt ggtatggaga atataaatga aaaagcgaag
     4441 atgttatata tacacaaggg tatctacgtc tatgcaggta gaaggataca gcttggaagc
     4501 acaaaaagaa agattgagaa aatatgcaga ttttcaggaa atggtgattg tcggagagta
     4561 ttcggatgag ggaaagtcag gaaaaagtat tgaaggacgg caggaattta tgcaaatgct
     4621 tcgggatatt gaagaaggta aagatcgtgt agagtttgtc ttggtatata agttatcccg
     4681 ttttggaaga aatgctgcag atgtacttaa ttctttgcag agaatgcagg attttggagt
     4741 aaatttgatc tgtgttgaag atggtataga cagttctaaa gacagcggta agctgatgat
     4801 ttctgttctg tctgcggtag cagaaattga acgtgaaaat attctggtac agacaatgga
     4861 ggggcgcaga caaaaggcga gagatggcaa atggaacggc ggatttgctc cgtacggata
     4921 taagctgtta aatggagaac tggaaattgc agaagatgag gcagaaatca ttcgtattat
     4981 ttttgataaa tatattcata ccaatatggg agtaaatggc attgcagcct atctgaatga
     5041 gcatggctac aagaagaaaa agagacagaa taatacgttg gatgcgtttg ctacttcttt
     5101 tataaagggt gtccttgata atcctattta ttgtggt
//
LOCUS       DBMDXG010000110         5090 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4039_length_5090_cov_1.294849, whole genome shotgun sequence.
ACCESSION   DBMDXG010000110 DBMDXG010000000
VERSION     DBMDXG010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4039_length_5090_cov_1.294849"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="ACZPD3_04385"
     CDS             complement(<1..469)
                     /locus_tag="ACZPD3_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1415275.1"
                     /translation="MTEITKSTEAKKDEMQVEKEKLESVKRADAAVKTMHDFTSPEVL
                     YKELINSVLKYHPSTDISMIEKAYKVASEAHEGQKRKSGEPYIIHPLCVAIILADLEL
                     DKETIVAGLLHDAVEDTWMTYEEVEKEFGSEVALLVDGAPVAVFAGDAKNHAFQ"
     gene            complement(507..1031)
                     /locus_tag="ACZPD3_04390"
     CDS             complement(507..1031)
                     /locus_tag="ACZPD3_04390"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR1415276.1"
                     /translation="MKKIEDYVLSIPDFPEPGIIFRDITSVLQDADGLQLAIDSMQDC
                     LKDIDVDVIAGTESRGFIFGVPIAYNLHKPFVPIRKKGKLPRETVSVSYDLEYGSAEI
                     EMHKDSIKPGQKVVIVDDLIATGGTIEAAIKLVEQLGGEVVKVVFLMELAGLKGRERL
                     NGYDVASVICYDGK"
     gene            complement(1179..2891)
                     /gene="recJ"
                     /locus_tag="ACZPD3_04395"
     CDS             complement(1179..2891)
                     /gene="recJ"
                     /locus_tag="ACZPD3_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR1415277.1"
                     /translation="MEKWVIAAKRADFNQIGQQFHIDPVIARLIRNRDVIGEDKIREY
                     LSGTVQELPSPWLMKDMEKAVDILEKKISQKAKVRIIGDYDIDGVTSTYILMKGLARI
                     GADVDTYIPDRVADGYGIHAHLIERAETDRIDTIVTCDNGIAAAAEIQMAKDKGMTVI
                     ITDHHEVPYREEKGERQMVLPPADAILNPKQYDCPYPNKNLCGAVVAFKYIAALYERF
                     GVPAEELEDYYELAAIATVGDVMDLQGENRILVKEGLCRLKNTKNQGLQELIRANSLE
                     DAKITAYHIGFVLGPCINASGRLDTAARSLALLNAKTKEEAARLAGDLTALNQSRKAL
                     TEKGKEEAIRIIETTELKNDRVLVVYLPDCHESLAGIIAGRIREKYHRPAFVLTGGET
                     SAKGSGRSIESYSMYEELVKCADLMIQFGGHPMAAGLSIEEKNIEEFRRRLNVNCTLT
                     DEELRPKIVIDVPMPVSYITKELVEQISLLEPFGKGNTKPVFAQKNLRVLDHSIIGKN
                     KNVVKLKLLDPQGISVEGIYFGEAEDFVNFIREKDSISVTYYPEINRFRGRESLQIII
                     QNYC"
     gene            complement(2973..>5090)
                     /gene="secD"
                     /locus_tag="ACZPD3_04400"
     CDS             complement(2973..>5090)
                     /gene="secD"
                     /locus_tag="ACZPD3_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR1415278.1"
                     /translation="PGVTDADTILNDLGKPGSLYFITKEDADGNQNFTTGQDGYVLSR
                     TMDEIKESGSVVLEGSDVADAQGGAIQNQNTSAKEYVVSLTLTSDGENSGKTKFAEAT
                     KNNVGKQIAIVYDNNIISAPNVNEEISGGKAQISGMSDLEEAQNLASYIRIGSLGLEL
                     EELRSSVVAAQLGEEAISTSVLAGAIGLIIVIIFMIIAYRVPGVVAGIALILYTSLML
                     ITLNAFDITLTLPGIAGIILGIGMAVDANVIIYARIREEIGAGVSVRNSIKSGFSKAF
                     SAIFDGNITTLIAAFVLMWLGSGTVKGFAYTLALGIVISMFTALVVSRLIVNALYAVG
                     VRDPKFYGSAKERKAVDFLGKKKVFFAISIILILCGPAAMFANSHAGNKALNYSLEFS
                     GGTSTTVTFNEDMDIKTIDSEVTPVVEEVTGDKNVQPTKVVGTNQVVIKTRSLNQSER
                     EALQSALVEKFGVDDSTISTESISSTVSSEMRRDAIVAVIVATICMLLYIWFRFKDIR
                     FASSAVLALLHDVLVVLAFYAIARVSVGNTFIACMLTIVGYSINATIVIFDRIRENLH
                     SGSREKLAEIVNTSITQTLTRSIYTSFTTFVMVAVLYIMGVSSIREFAAPLMVGVLVG
                     AYSSVCITGALWYVMKKKFAGKGQPAIAAASASAKSSSKPKKARDVSQKDPTQPKKKN
                     RKRVAERLAAEEAAKKQQNDDAE"
BASE COUNT         1372 a         1260 c          881 g         1577 t
ORIGIN      
        1 gctggaaggc gtgattcttc gcatcgccag cgaatacagc gacgggcgca ccatccacaa
       61 gaagtgcaac ctctgaacca aattccttct caacttcctc ataggtcatc caggtatctt
      121 caactgcatc atgaagaaga cctgcaacta tcgtttcttt gtcaagttcc agatctgcca
      181 gaatgatggc aacacacaaa ggatgaatga tatatggctc tccagacttt ctcttctgtc
      241 cttcatgagc ttcacttgca accttatatg ccttctctat catggaaata tctgttgatg
      301 gatgatactt cagaacactg tttatcagtt ctttataaag cacctccgga cttgtaaaat
      361 catgcatggt ctttactgcc gcatctgctc tctttacaga ttccagcttc tctttttcaa
      421 cctgcatttc atcttttttt gcttctgtac tttttgttat ctctgtcata tgctcccacc
      481 tgcctgtcct gcacaatcag ctgtgcttat ttaccatcat aacaaattac agacgctaca
      541 tcatacccat tcagtctttc gcgtcccttt aaacctgcaa gttccatgag aaatacaacc
      601 ttgacaacct caccgccaag ctgttccacc agcttgatcg ctgcttcgat cgtaccgcct
      661 gttgcgatca gatcatcaac gataaccact ttctgacctg gttttatgga atctttatgc
      721 atttcaatct ctgcactgcc atattccaga tcatagctta cagatactgt ttcgcgggga
      781 agtttacctt ttttacgaat cggtacaaat ggtttatgaa gattatatgc gatcggtaca
      841 ccaaagataa agcctctgga ctctgttcct gcaatcacgt ccacatctat atccttaaga
      901 cagtcctgca tggagtcgat tgccaactgc aggccatccg cgtcctgcag aacacttgta
      961 atatctctga aaataatccc cggttcagga aaatccggta tacttaatac ataatcttca
     1021 attttcttca ttttatttct cctccagtgt gtgttatatt gctgttcgct cataaatatt
     1081 ttatagtata tcaccttttt atttcagaat caagttactg ttaagccctg acatttgtat
     1141 ttccaggctt accggcggtt acaaaccaag cggtaatatt aacagtaatt ctgaataata
     1201 atctgaagac tctctcgtcc cctgaatctg ttgatctccg gataataagt aacagaaata
     1261 ctgtcttttt ccctgataaa gttcacaaaa tcctctgctt ctccgaaata aatgccttcc
     1321 actgaaatcc cctgcggatc gagaagtttt agttttacta catttttatt tttcccgata
     1381 atactgtgat caagcactct gagatttttc tgcgcaaaga ccggttttgt attccctttt
     1441 ccaaagggtt ccagaagcga aatctgttct accagttctt ttgtaatata agacacaggc
     1501 atcggcacat caatcacgat cttaggacgc aattcttcgt ccgtcaacgt acagttgaca
     1561 ttgaggcgtc tccggaattc ttctatattt ttttcttcta tagacagacc tgctgccatc
     1621 ggatgacctc caaactgtat catcaggtct gcacatttta ccagttcttc atacatagag
     1681 taactttcta ttgatcttcc gcttcctttt gcacttgtct caccgcccgt caatacaaag
     1741 gccggcctgt gatatttttc cctgatcctg cctgctataa taccggcaag actttcatga
     1801 caatccggaa gataaactac cagtactctg tcatttttca gctctgtagt ctctataatc
     1861 cggattgcct cttcttttcc tttctcagtg agagcttttc ggctctgatt aagagctgta
     1921 agatctccgg caagccttgc tgcttcttcc tttgtttttg cattgagaag tgcaagagaa
     1981 cgggcagctg tatccagtct gccactggca ttgatacatg gtcccagcac aaatccgata
     2041 tgataagctg tgattttagc atcttccaga gaatttgctc ggatcagttc ctgcaatcct
     2101 tgatttttgg tatttttcag acggcacagc ccttctttta ccagaatcct gttctctccc
     2161 tgaagatcca taacatctcc tactgttgca atagccgcca gttcataata atcttccagc
     2221 tcctctgccg gaacaccaaa tctctcgtaa agtgctgcaa tatatttaaa ggccaccact
     2281 gccccacaca ggtttttatt cgggtatgga caatcatatt gtttcggatt caaaatcgca
     2341 tctgccggcg gaagcaccat ctggcgttct cccttttcct ccctgtaagg aacctcatga
     2401 tgatctgtaa taatcactgt cattccctta tcctttgcca tctggatttc agcagccgca
     2461 gcaattccat tatcacaggt cacgatcgta tcgatccggt ccgtttccgc tctttctata
     2521 agatgtgcat gaatcccata tccgtctgca actctgtccg gaatataagt atccacatct
     2581 gctccgatcc tcgcaaggcc tttcattaaa atgtatgtag aggtcacacc atcaatatca
     2641 taatcaccaa taatacggac ttttgctttc tgcgatattt ttttctccag gatatctaca
     2701 gctttttcca tatccttcat cagccaggga gaaggaagct cttgtactgt tcctgacaga
     2761 tactctctta tcttatcttc tccgatcaca tctctgttcc tgatcagtct cgcaatcacc
     2821 ggatcaatat gaaactgttg accgatctgg ttaaaatctg ctctttttgc tgctattacc
     2881 catttctcca tatattttct ctcctcataa aaatatcccg gagttttacc ccgggatatt
     2941 tctgtctgcc tgtttttcag acaattattg ttttattcag catcatcatt ctgctgtttt
     3001 ttagctgctt cttcggcagc cagacgttca gctactcttt ttctgttttt cttctttggc
     3061 tgagtcggat ctttctgtga tacatcacgt gctttcttcg gctttgaaga ggactttgca
     3121 gatgcggaag ctgcagcaat tgccggctgt ccttttcctg caaatttctt tttcatcaca
     3181 taccacaacg cacctgtaat acatacagaa gaatatgcac cgacaagaac accaaccatc
     3241 agaggtgctg caaattcacg gatagaagaa acacccatga tataaagaac agctaccata
     3301 acaaaagttg taaaagaggt ataaatactt cttgtaagag tctgagtgat acttgtgttt
     3361 acaatttctg ccagtttttc tctggaacca ctgtgaaggt tctcacgtat acggtcaaaa
     3421 ataacgattg tagcattgat agaataacct acgattgtaa gcatgcaggc aataaatgta
     3481 ttaccgacag atactctggc aatagcatag aatgcaagta caaccagaac atcatgcaga
     3541 agcgccaata ccgcactgct tgcaaaacgg atgtctttaa atctgaacca gatataaaga
     3601 agcatacaga ttgtagctac tataacggct acgatcgcat cacgacgcat ttcactactt
     3661 actgtagaag aaatactttc tgtggaaatc gtgctgtcat ctacaccaaa tttctcaacc
     3721 agtgcactct gcagagcttc acgttcgctc tgattcagag aacgtgtctt gatcacaacc
     3781 tgatttgttc cgactacctt tgtaggctga acatttttat ctcctgtcac ctcttctaca
     3841 acaggagtta cttcagaatc aatggtctta atatccatat cttcattaaa tgtaacagtc
     3901 gtagaagtac cgcctgagaa ctcaaggctg taatttaacg ctttgtttcc tgcatgcgaa
     3961 ttagcaaaca tcgctgccgg accgcagagg atcaggataa tggaaatagc aaaaaatact
     4021 ttcttctttc caaggaaatc aactgcctta cgttcttttg cagatccgta gaatttcgga
     4081 tcacgtacac caactgcata aagtgcattt acaatcagtc tggatacaac cagtgctgta
     4141 aacatggaga tcacgatacc aagggcaagt gtataagcaa atcccttaac agtacctgaa
     4201 ccaagccaca tcagcacaaa ggctgcgatc agtgttgtaa tatttccatc aaagattgca
     4261 gagaaagctt tgctgaaacc ggacttaata gaattcctta ctgaaactcc tgcaccaatt
     4321 tcctctctga ttcgggcata aataataaca ttggcatcca ccgccatacc gataccaaga
     4381 ataatacccg caatacccgg aagagtaaga gtaatatcaa aggcattcag cgtgatcagc
     4441 attaatgatg tataaagaat cagtgcgata cctgcaacta caccaggtac gcggtatgca
     4501 atgatcataa agataataac gatgatcaga ccgatcgcac ctgcaagaac acttgtagag
     4561 attgcttctt caccaagctg tgcagctaca acactggaac gaagttcttc cagttccagt
     4621 cccagagaac cgatacggat ataagaagca aggttctgtg cttcttccag gtcagacatt
     4681 ccgctgatct gtgcttttcc gccggaaatc tcttcattta cattcggagc actgataatg
     4741 ttattgtcat agacgatagc gatctgcttg cctacattat tctttgtagc ttctgcaaat
     4801 tttgttttac cactgttttc tccatcagaa gtaagagtaa gacttaccac atattccttt
     4861 gcacttgtgt tctgattctg gatcgcacca ccctgggcat ctgctacatc agaaccttcc
     4921 agaacaacac ttccggattc cttgatctca tccatagttc tggagagaac atatccatcc
     4981 tgtcctgtag taaagttctg gtttccgtca gcatcttctt ttgtgatgaa atacagggaa
     5041 cctggtttac ccagatcatt caaaatagta tctgcatctg taacaccagg
//
LOCUS       DBMDXG010000111         5072 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4053_length_5072_cov_2.164353, whole genome shotgun sequence.
ACCESSION   DBMDXG010000111 DBMDXG010000000
VERSION     DBMDXG010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4053_length_5072_cov_2.164353"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..3656)
                     /gene="rpoB"
                     /locus_tag="ACZPD3_04405"
     CDS             complement(<1..3656)
                     /gene="rpoB"
                     /locus_tag="ACZPD3_04405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR1415279.1"
                     /translation="MEKNRIRSVKTGKSMRMSYQRQEEVLEMPNLIEVQRDSYKWFLD
                     EGLREVFDDISPIADYSGKLSLEFIDFTFDEKDAKYTIEQCKERDATYAAPLKVRVRL
                     INKETEEINEHEIFMGDLPIMTVTGTFVINGAERVIVSQLVRSPGIYYAIAHDKLGKR
                     LFSSTVIPNRGAWLEYETDSNDVFYVRVDRTRKVPITVLIRALGVGSNAEIIDLFGEE
                     PKILASFTKDTSTNYQEGLLELYKKIRPGEPLAVESAESLINAMFFDPRRYDLAKVGR
                     YKFNKKLHLNRRIVGHRLAEDVVDASTGEILAEEGTVVTKEMANTIQNSAVPYVWILG
                     EEDRRIKVLSNLMVDIRHYLPEIEDPKSIGVTEAVYYPVLENILEENDTLEDRIAAIH
                     RDIHDLIPKHITKEDIFASINYNMHLEYGLGNADDIDHLGNRRIRAVGELLQNQYRIG
                     LSRLERVVRERMTTQDTENISPQSLINIKPVTAAVKEFFGSSQLSQFMDQNNPLGELT
                     HKRRLSALGPGGLSRDRAGFEVRDVHYSHYGRMCPVETPEGPNIGLINSLASYARINQ
                     YGFIEAPYRKIDKSDPKNPRVTDEVVYMTADEEDNYHVAQANEPLDEEGYFIHKNVSG
                     RFREETQEYERHMFDYMDVSPRMVFSVATALIPFLQNDDANRALMGSNMQRQAVPLLT
                     TEAPVVGTGMEVKTAVDSGVCVVAKKSGTVLRSTSTDISIKNDDGTKDDYHLTKYLRS
                     NQSNCYNQKPIVFQGEHVEAGQVIADGPSTANGELALGKNPLIGFMTWEGYNYEDAVL
                     LSERLVMDDVYTSVHIEEYECEARDTKLGPEEITRDVPGVGDDALKDLDERGIIRIGA
                     EVRAGDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGEYGIIVDAKV
                     FTRENGDEMSPGVNQSVRIYIAQKRKISVGDKMAGRHGNKGVVSRVLPVEDMPFLPNG
                     RPLDIVLNPLGVPSRMNIGQVLEIHLSLAAKALGFNVATPIFQGANEHDIQDTLELAN
                     DYVNTEDFEEFREKYKDILAPDVMQYLDENKAHRALWKGVPISRDGKVRLRDGRTGEY
                     FDSPVTIGHMHYLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWAL
                     EAYGASYTLQEILTVKSDDVVGRVKTYEAIIKGDNIPEPGIPESFKVLLKELQSLGLD
                     VKVLKDDNTEVHLL"
     gene            complement(3866..>5072)
                     /locus_tag="ACZPD3_04410"
     CDS             complement(3866..>5072)
                     /locus_tag="ACZPD3_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016906.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR1415280.1"
                     /translation="NTFEDVFAGIGSHSMMFDKPYKNITISNNRFNNLKKRAIWCLNY
                     QDTVVTGNTMTNVGGGVYVRSVYTRNAHTVSGQEVSPEENQYAENILIADNQITVLEP
                     TVIDGKQWNGYGIWITGEVSLGSAGETIPSEDDDPENTANPTTNGIPAGRYIIRGVTA
                     RNNTISGNCDGIKFSLAEDCSCRGNTVRLSDSHTYNNMGISVAKGSNIRVSYNNLSGG
                     NGYGIYTVGTEASQKICRIYGNTVSGFMKDGILASGIAAGSRIEHNRVSTSAENGILV
                     KCIDKSVVDGNYSFKNKARGILLQRCESAMVADNFVSENVINGIELNIRSNHSSVQGN
                     VCGSNKKSGLRIAGSKGISADGNSFRSNRGYAAEFISSQISSYKGNRFEKNGYSNRIH
                     VRNSKIPKK"
BASE COUNT         1285 a         1236 c          996 g         1555 t
ORIGIN      
        1 tcaagaagat gtacttctgt attgtcatcc ttgagtacct tcacatccag accaagagac
       61 tgaagttctt ttaagagaac cttgaaggat tccggaatac ccggctcagg aatattgtca
      121 cccttgatga ttgcttcgta tgtcttaaca cgtccaacaa catcatcgga ttttactgtc
      181 aggatttcct gcagtgtgta ggatgcaccg tatgcttcca gtgcccaaac ctccatctca
      241 ccaaaacgct gtccaccgaa ctgtgcttta ccacccagtg gctgctgtgt taccagagag
      301 taaggacctg tagaacgtgc atggatctta tcatcaacca gatggtgcag tttcagatag
      361 tgcatgtgac caattgttac aggactgtcg aaatactctc ctgtacggcc gtcacgcaga
      421 cgcactttac cgtctctgga aatcggaaca cccttccaca gtgctctgtg ggctttgttc
      481 tcatccagat actgcattac atccggtgct aaaatgtctt tgtatttctc gcggaactct
      541 tcaaagtctt ctgtgtttac atagtcgttt gcaagttcca gtgtatcctg gatatcatgc
      601 tcgtttgcgc cctggaaaat aggagttgca acgttgaatc caagtgcttt cgcagcaagg
      661 cttaagtgaa tctccaggac ctgtccgata ttcattcggg aaggcacacc cagagggttc
      721 agcacgatat caagaggacg tccgttcgga aggaatggca tatcttcaac aggaagtaca
      781 cgggaaacaa cacccttgtt accatgacgt ccggccatct tatcaccaac ggaaatcttt
      841 ctcttctgtg cgatgtagat acgtacggac tggttaacac caggtgacat ctcatcgccg
      901 ttctctcttg taaatacctt ggcatctacg ataataccgt attcaccgtg aggtactttc
      961 agagaagtat cacgtacttc acgtgctttc tcaccaaaga tcgcacgaag aagtctctct
     1021 tctgccgtaa gctctgtctc tcccttagga gtaactttac caacaaggat atcgccagca
     1081 cgtacttcag caccgatgcg gataatacca cgctcatcca gatctttcag tgcatcgtcg
     1141 cccacgccag gaacatcacg tgtgatctct tccggtccca gtttggtatc acgggcttcg
     1201 cattcatatt cttcaatatg aacagatgta taaacatcgt ccataacaag tctttcactg
     1261 aggagtaccg catcctcgta gttataacct tcccatgtca tgaatccgat cagcgggttc
     1321 ttaccaagag caagttctcc gtttgctgtg gaaggaccat ctgcgataac ctgtcctgcc
     1381 tctacgtgct caccctggaa tacgattggt ttctggttgt aacagttact ctggttactt
     1441 ctcagatatt tagtcagatg gtaatcatct tttgtaccat catcattctt aatgctgata
     1501 tctgtagatg tggaacgaag tacagtacca gatttctttg caactacgca gacaccggag
     1561 tcaactgctg tcttcacttc cataccagta ccaactaccg gagcctctgt tgtcagaagc
     1621 ggcacagcct gacgctgcat gtttgatccc atcagcgcac ggttggcatc gtcgttctgc
     1681 aggaacggga tcagggcagt agcgacggag aataccatac gaggagatac atccatgtaa
     1741 tcaaacatat gacgctcata ctcctgagtc tcttcacgga aacgaccgga tacattctta
     1801 tgaatgaaat atccttcttc gtccagaggc tcattcgcct gtgctacatg gtaattatcc
     1861 tcttcatcag cagtcatata tacaacctca tctgttacac gaggattctt aggatctgat
     1921 ttgtcgatct tgcggtatgg tgcctcaata aatccgtact gattgattct tgcataagat
     1981 gcaagagagt tgatcagacc gatgttagga ccttcaggtg tctcgacagg gcacattctt
     2041 ccgtaatggg agtaatggac atctcgaacc tcgaatccgg ctctgtctcg tgacaaaccg
     2101 ccaggtccca gggcggaaag acgtctcttg tgtgtcagct caccaagagg gttgttctga
     2161 tccataaact gtgacagctg ggaagaaccg aagaactctt tcactgctgc agttacaggt
     2221 ttaatattga tcagggactg tggagaaata ttctctgtat cctgagttgt cattcgctca
     2281 cgaaccacac gttccagtct ggaaagaccg attctgtact ggttctgaag cagctcacct
     2341 accgcacgta tacgacggtt gcccaggtgg tcgatatcat cggcattgcc aaggccatat
     2401 tccagatgca tattgtagtt aatagaagca aagatgtctt cttttgtgat atgcttcggg
     2461 atcagatcat ggatatctct gtggattgct gcaatcctgt cttctaatgt atcattttct
     2521 tccagaatgt tttcgagaac agggtagtat actgcttctg taacaccgat gctctttgga
     2581 tcttcaattt ccggaagata gtggcggata tcaaccatta agttagaaag aaccttgatt
     2641 ctacggtctt cctcgccaag gatccataca tatggaacag cgctgttctg gatggtattt
     2701 gccatttctt ttgtcacaac agttccttct tctgccagga tttcgcctgt agaagcatca
     2761 acaacatcct cagccagtct gtgaccaacg attcttctgt tgaggtggag cttcttgtta
     2821 aatttataac gtccaacttt cgcaaggtca tatcttctcg gatcaaagaa cattgcattg
     2881 ataaggctct ccgcactctc aactgcaagt ggctcacccg gacggatttt cttgtataat
     2941 tcaagaagac cttcctgata gttggtggat gtatcctttg taaaacttgc aaggatcttt
     3001 ggctcttcac cgaagagatc aataatctct gcattgcttc ctacaccaag tgctcggatc
     3061 agaacagtga tcggaacctt tcttgttctg tcaacacgta catagaaaac atcattagag
     3121 tctgtctcat attccagcca tgcacctctg ttcgggataa cagtactgga gaacagtctc
     3181 ttaccaagtt tatcatgcgc tattgcgtaa tagattccag gggaacgtac taactggctg
     3241 acaattactc gctctgcacc attgatgaca aaggtacctg taacagtcat gatcggtaaa
     3301 tctcccatga aaatctcgtg ttcgttgatt tcctctgttt ctttgttaat cagtctcact
     3361 cgaaccttca aaggtgcggc gtaagttgcg tcacgttctt tacactgttc gatagtatat
     3421 ttcgcatcct tctcgtcaaa tgtaaagtcg atgaattcca ggctgagctt tccgctgtag
     3481 tccgcgatcg gagaaatatc atcaaagact tccctaagtc cttcatcaag aaaccattta
     3541 taagaatccc tctggacttc gatcaggttt ggcatttcca ggacttcttc ttgtctctgg
     3601 tagctcatac gcatgctttt tccagttttt acagaacgaa ttctgttttt ttccattgac
     3661 gtttcacccc tgttttttct ttaaatttat tataacttga ccctttttgc caaaagacat
     3721 aaaggcacat cttgccataa tatagcattc ttcactatag cacagcaagt tgcgcctgtc
     3781 aatcactttt ttgatatttt tttaattttt tcaaataatt gcagcagtcg ctatttcgtt
     3841 actgtgaaca gcgtgaacag taacgctatt tcttcgggat tttactgttt cttacatgga
     3901 tacgatttga atatccattt ttctcaaaac gattgccctt ataactgctg atttgggaac
     3961 tgataaattc cgccgcatat cctctgttgc tgcggaagga atttccgtct gccgaaatcc
     4021 cctttgatcc ggcaattcgc agacctgatt ttttattgga accacagaca tttccctgca
     4081 cagaagaatg attgcttctt atattgagtt caattccatt gatcacattt tcagaaacaa
     4141 aattatctgc caccattgct gactcacatc tctgcagaag aattcctctt gccttatttt
     4201 taaaactgta attgccatca accacactct tatcaataca ttttacaagt atgccatttt
     4261 ccgcactggt actgactctg ttgtgttcaa tacggcttcc tgctgcaatc cctgaagcca
     4321 gaattccatc tttcataaat ccggagactg tatttccgta aatccggcaa attttctgtg
     4381 aagcctctgt tccgactgta taaatcccat atccgtttcc gccggaaaga ttgttatagg
     4441 aaactctgat attgcttccc ttggcaacac tgatccccat attgttgtag gtatgactat
     4501 cagaaagtct gactgtgttc cccctgcatg aacagtcttc tgcaagagaa aatttaatcc
     4561 cgtcgcaatt tccgctgatc gtattatttc tggctgtgac tccgcgaata atatatcttc
     4621 ccgccggaat tccattggtc gtcggatttg ctgtattctc aggatcatca tcttcagaag
     4681 gaatagtttc tcctgctgag cccagagata cctcgcctgt gatccagatt ccatatccat
     4741 tccattgttt gccatctatt acggttggtt ccagaactgt gatctgatta tcagcgatta
     4801 gaatattctc tgcatactga ttttcctccg gagatacttc ctgaccgctg acagtatgtg
     4861 catttctggt ataaacagaa cgcacataaa caccaccgcc cacatttgtc atggtatttc
     4921 ccgtcacaac cgtatcctga taattcaggc accatattgc tctctttttc agattattaa
     4981 accggttgtt actgatcgtg atatttttat atggtttatc aaacatcatg ctgtggcttc
     5041 caatgccggc aaatacatct tcaaaggtat ta
//
LOCUS       DBMDXG010000112         5046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4079_length_5046_cov_2.262083, whole genome shotgun sequence.
ACCESSION   DBMDXG010000112 DBMDXG010000000
VERSION     DBMDXG010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_5046_cov_2.262083"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(111..842)
                     /locus_tag="ACZPD3_04415"
     CDS             complement(111..842)
                     /locus_tag="ACZPD3_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415281.1"
                     /translation="MGIITILWEKWVEFRRDFYKITLAAMIAPLMYLIVFGMGIQTTS
                     HGQPYLNFLIPGVVSLTTMNGSFNAIAQNLNVQRLYEKAFDQVMISPTPLWQFIAGQI
                     IGGSLRGLYAGGIILLLTAPIETGLIFNGWSFLVMFLNGAVFSAIGVVVSFLAKNHAD
                     VPRFSNYIIMPMAYLCNTFFSTEKLPGFVGKFVSALPLSQTSHLMRSISSGEAVNYTG
                     IGILLLYLLVFTVAASWFIYKKKNL"
     gene            complement(916..1758)
                     /locus_tag="ACZPD3_04420"
     CDS             complement(916..1758)
                     /locus_tag="ACZPD3_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415282.1"
                     /translation="MIETKDLTKTFDNFTAVDSLNLKIEKGEFFGLLGPNGAGKTTTI
                     SLLSTLLLPTKGEILIDGQQLTRQRPDLKRKISVITQEYSMRQDMNMDEIMEYQGRLY
                     FMPRKQIKERTEELLEFCDLLKFRKRTVRKLSGGMKRKLMVCRALLTDPEILLLDEPT
                     AGMDALSRRQMWNLLRKLNEKNLTILLTTHYMEEAQNLCNRVALMDHGKLEEVSTPQA
                     LIESLGAYAVDEMTADGTQNHYFHTRQEAIRYLEELTGQASLRETTLEDVFVERAGKH
                     LISK"
     gene            complement(1755..2450)
                     /gene="cobI"
                     /locus_tag="ACZPD3_04425"
     CDS             complement(1755..2450)
                     /gene="cobI"
                     /locus_tag="ACZPD3_04425"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIR1415283.1"
                     /translation="MRGILYGVGVGPGDPELMTLKAVRLIKENDIIAVPGAEVRETVA
                     YKIAVQAVPELADKELLPIYMPMTHDKAELELNHEKGAKALEAALDTGKNVVFLTLGD
                     PTIYSTFSYVQKRVEADGYKTRLVSGITSFCATAARLNIPLTEWNQPLHVQPAVHRLD
                     SELDQPGTYVLMKSGRKMNQVKEILERSGREVRMVENCGMPDEHIYNGVEEIPDDAGY
                     YSLIIAKEKAEEK"
     gene            2696..3214
                     /locus_tag="ACZPD3_04430"
     CDS             2696..3214
                     /locus_tag="ACZPD3_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415284.1"
                     /translation="MRKIFAVLAALICCLSVWTSVPAAQAFTLDKEDGEYSIQVELEG
                     GSGKASVTSPTLITVKNGEVTADIQWSSSNYDYMIVDGKKYLPVNEEGTNSEFQIPVT
                     IMDESMPVIADTTAMGTPHEINYTLTFYSDSIGSKSQMPQEAAKRVVAVALVIIVGGG
                     ILNYFVNKRNRC"
     gene            complement(3296..4606)
                     /locus_tag="ACZPD3_04435"
     CDS             complement(3296..4606)
                     /locus_tag="ACZPD3_04435"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR1415285.1"
                     /translation="MYQFNRKEYEERMKWYQDARFGMFIHWGLYSIPARGEWVRSTEE
                     MPEEEYLPFMKEFDARDYNPKQWAKEAKAAGMKYVVLTAKHHDGFCLFDSKYTDYKVT
                     NTPAGRDLIKEYVEALREEGLKVGLYFTLLDWHHPDYPHYGDRIHPMRNHPECSNENR
                     DFSRYIEYMYNQVEEVCTNYGKIDIMWFDFSYDDMRGEKWGATRLIDMVRRLQPGIII
                     DNRLEVSGEGRGSLYECDPTPYHGDFISPEQIIPPEGICDKEGNPMVWEACFTMNNNW
                     GYCANDHYYKPASMLIKKLVECVSKGGNMILNVGPDAKGKFPKESSAILAEIGRWMDK
                     NHDSIYGCAPAADIPKPDYGRITRNGNKYYVHIYENTLGPLPLIGFDKNKIVKVRALD
                     DGHEVPISTLWVHSDYPDIAFVDLGPDPVLPDPVDYVLEVEMAE"
     gene            complement(4734..>5046)
                     /locus_tag="ACZPD3_04440"
     CDS             complement(4734..>5046)
                     /locus_tag="ACZPD3_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1415286.1"
                     /translation="TEHILNEMMEQEEAFAGYAEQIIFVVRSKEALETPTLSKALAKL
                     KNIQIYYDDFSEIINTLGRRMYVDPDKLPLIIVTNGTLNGIYATSGYNVGTGDMLLRL
                     M"
BASE COUNT         1300 a         1213 c          994 g         1539 t
ORIGIN      
        1 agaaattgat aatcttcatg ttctcacaaa taccgctgat acttctgagt agcagtcagt
       61 cggatacaaa ataagtcagg gaggagcaca ggctcctccc tgtcagtcag ttacaggttt
      121 ttcttcttat aaataaacca tgatgcagct acagtaaaca ccagcagata aagaagcaga
      181 atcccaatac cggtgtaatt caccgcctca cctgatgaaa tgcttctcat cagatggcta
      241 gtctgggata atggcaaggc tgatacaaac ttgcctacaa atcccggaag tttctctgtt
      301 gagaaaaagg tgttacacaa atatgccata ggcataatga tataattgga aaacctcggc
      361 acgtctgcgt ggttttttgc cagaaaggag accaccacgc cgatggctga gaataccgct
      421 ccgttcagaa acatcaccag aaaggaccat ccgttgaaga tcagtcctgt ctcaataggt
      481 gctgtaagaa gcagtatgat tcctcctgca tacagaccgc gcagacttcc cccgatgatc
      541 tgtccggcaa taaactgcca taatggtgtg ggggagatca tcacctgatc aaaagccttc
      601 tcatacagtc tctgtacgtt taggttctgc gcaatggcgt tgaagcttcc gttcatagtt
      661 gtcagtgata ccacacccgg aatcagaaag ttcaggtatg gctgtccatg agaagtggtc
      721 tggattccca ttccgaatac gatcagatac attagcggtg caatcattgc cgccagtgtg
      781 attttataga aatcccggcg gaattctacc cacttctccc ataatattgt tataattccc
      841 atacattttt ctccagatta ttctaacttt tttcgcaacc tccgcagggg acttccctga
      901 ttcagttaat atctgctatt ttgaaatcaa atgcttcccg gctctttcta caaagacatc
      961 ttccagtgtt gtctcgcgaa gagacgcctg ccctgtaagc tcttccagat accggattgc
     1021 ttcctgtcgt gtatggaaat agtgattctg tgttccatct gctgtcatct cgtctactgc
     1081 ataagcacca agactttcga tcaatgcttg cggagtactt acctcctcca gctttccatg
     1141 gtccatcagt gccacccggt tacagaggtt ctgggcttct tccatatagt gagtggtcag
     1201 aaggatcgtc agatttttct cattaagctt acgcagaaga ttccacatct gtcttctgga
     1261 aagagcatcc atacctgctg tgggttcatc caaaagcaat atctccggat ctgtcagtaa
     1321 tgcccgacag accatcagct tacgcttcat tccgccggaa agcttacgga ctgtacgttt
     1381 gcgaaacttc agcaggtcac agaactccag aagttcttct gttctttctt ttatctgctt
     1441 tcgcggcata aaatagagcc gtccctggta ttccataatc tcatccatat tcatatcctg
     1501 acgcatagaa tattcctggg tgatcacact gatttttctt ttcagatcag gacgttgtct
     1561 cgtaagctgc tgaccgtcta tgaggatttc tccctttgtc ggaagaagaa gggttgagag
     1621 caggctgatg gtcgttgtct tgcctgctcc gtttggtccg agaagaccaa aaaactcacc
     1681 tttttcaatt ttcagattca gggaatccac tgctgtaaaa ttgtcaaaag ttttcgtcag
     1741 atcttttgtc tctatcattt ctcttcggct ttctctttag caatgatcag ggaataatat
     1801 cctgcatcat ccggaatttc ctctacaccg ttgtaaatat gttcatccgg cataccacaa
     1861 ttctctacca tacggacttc gcgaccgctt ctttcaagga tttcctttac ctgattcatt
     1921 tttctgcctg atttcatcag tacataggtt cccggctgat caagttcact gtcaaggcgg
     1981 tgaactgcag gctgtacatg aagaggctgg ttccactctg taagcgggat attcaggcgt
     2041 gctgctgttg cgcagaagga agtgatgccg cttaccagtc ttgtcttata tccatctgct
     2101 tctactctct tctgcacata tgaaaatgtt gaatagatag tcggatctcc aagtgtcaga
     2161 aatactacat tcttacctgt atcaagtgct gcttccagcg ccttggctcc tttttcatgg
     2221 ttcagttcca gttctgcctt atcgtgtgtc attggcatat agattggcag aagttctttg
     2281 tctgcaagct caggtactgc ctgtactgcg atcttatatg caactgtttc acgcacctct
     2341 gcgcccggta ctgcaataat atcattctct ttgatcaggc gcactgcctt taatgtcata
     2401 agttccggat ctccggggcc aactcctact ccgtataaaa ttcctctcat taccatatcc
     2461 tcctgtgatt tgcatataca tttttatgat atcagaattg cagaagctgc ttcgcagcaa
     2521 agcaattcac agatattcat ttattgtgct cactgttcac tacgttctca atgatgttgc
     2581 gtgcggacag tactttttat tatactaaaa caggataact ttttcaacaa agtattgaca
     2641 attaatttca attgtgggat gatgaaaatt atcgaattta agtgaggaaa gaaatatgag
     2701 aaagattttt gcagttctgg cagctttgat ctgctgtctg tcagtatgga cttctgttcc
     2761 tgctgcacag gctttcactc ttgataaaga agatggggag tattccatcc aggtagagct
     2821 ggaaggcgga agcggcaaag ccagtgtcac ttcaccgact ttgatcactg tgaaaaacgg
     2881 cgaagtaact gccgatatcc agtggagcag ttctaattat gactacatga tcgttgacgg
     2941 taaaaaatat ctccctgtca atgaggaggg aacgaattct gaattccaga tcccggttac
     3001 cattatggat gagagcatgc cggtaatcgc agacactacc gccatgggta cccctcatga
     3061 gatcaattat accctgactt tctattccga ttccatcggt tccaaaagcc agatgccgca
     3121 ggaagcagcg aaacgtgttg ttgctgttgc acttgttatt atcgtaggcg gtgggattct
     3181 gaattatttc gtaaacaaga gaaaccgatg ctgaaccagg cagacacctg catctcaatt
     3241 cggttaagaa atacaaaaaa gctgccatac ggcagctttt ttgtattcta tagctttatt
     3301 cagccatctc cacttccagc acatagtcta caggatccgg cagtacagga tccggaccaa
     3361 ggtcaacaaa tgcgatatcc ggataatcgc tgtgcaccca taaagtagag attggcactt
     3421 catgtccgtc atccagggca cgtactttca caatcttatt cttatcaaat ccaatcagcg
     3481 gcagtggtcc taatgtattc tcataaatat ggacataata cttgtttcca tttctggtga
     3541 tacgtccgta atccggtttc gggatatctg ctgccggagc gcatccatag atactgtcat
     3601 ggttcttatc catccagcgt ccgatttccg ccaggatcgc ggaggattct ttcgggaatt
     3661 tacctttcgc atcaggtcct acattcagga tcatatttcc acctttggaa acacactcta
     3721 ccagcttctt gatcagcatg gaagcaggtt tgtaatagtg gtcatttgca cagtagcccc
     3781 agttattgtt catggtgaag catgcttccc ataccatcgg attcccttct ttgtcgcaga
     3841 ttccttccgg cgggatgatc tgttccgggc ttataaaatc gccatgatac ggtgtcggat
     3901 cgcattcata cagagatcct cttccctcac cactgacttc cagacggtta tcaatgatga
     3961 ttcccggctg taatctgcgg accatatcta tcagtctggt tgctccccat ttctcgcctc
     4021 gcatatcgtc ataggaaaag tcaaaccaca taatatcgat ttttccataa tttgtgcaga
     4081 cctcttccac ctgattgtac atatactcta tataacgaga aaaatctctg ttttcattac
     4141 tgcattccgg atggtttctc ataggatgga tgcggtctcc gtaatgagga tagtccggat
     4201 gatgccagtc aagtaaggtg aaatacagac cgactttcag accttcttcg cgaagagctt
     4261 ccacatattc tttgatcaga tctcttcctg caggtgtatt ggtaacttta taatctgtgt
     4321 atttactgtc aaataaacag aaaccatcat ggtgttttgc agtcagaacc acatatttca
     4381 tacctgctgc ctttgcctct tttgcccact gtttcggatt atagtctctg gcatcaaatt
     4441 ctttcataaa cggaaggtat tcttcctcag gcatctcctc tgtactgcgt acccattctc
     4501 ctcttgcagg aatagaatat aatccccagt ggataaacat tccgaaacgg gcatcctgat
     4561 accatttcat acgttcttca tattcttttc tgttaaactg atacatgatt tttatctcct
     4621 ttatgtaaat gcaaattcaa tatccaatcc tttacatcta tacaataatg taatccggca
     4681 ataccagaag atgctcacag gtgtcatctc cgggcatctt atttatcgct ctgttacatt
     4741 aatctaagca gcatatcccc ggttcctaca ttgtaaccgc tggtcgcata aatcccattc
     4801 agggttccat tcgtcacaat aatcagcgga agtttgtccg gatctacgta catacgcctt
     4861 cccagagtat tgataatctc tgagaaatca tcataataaa tctgaatatt ttttaacttt
     4921 gcaagtgctt tggacagtgt cggtgtctcc agtgcttctt tgcttctcac tacaaaaata
     4981 atctgctctg catatcctgc gaaagcctct tcctgctcca tcatttcatt taagatatgc
     5041 tcagta
//
LOCUS       DBMDXG010000113         5046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4080_length_5046_cov_2.055612, whole genome shotgun sequence.
ACCESSION   DBMDXG010000113 DBMDXG010000000
VERSION     DBMDXG010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4080_length_5046_cov_2.055612"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /gene="rpsI"
                     /locus_tag="ACZPD3_04445"
     CDS             complement(<1..125)
                     /gene="rpsI"
                     /locus_tag="ACZPD3_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR1415287.1"
                     /translation="MANTKYYGTGRRKSSVARVYLVPGTGKITINKRDIDEYLGL"
     gene            complement(150..578)
                     /gene="rplM"
                     /locus_tag="ACZPD3_04450"
     CDS             complement(150..578)
                     /gene="rplM"
                     /locus_tag="ACZPD3_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR1415288.1"
                     /translation="MQTYMANPDKIERKWYVVDADGCTLGRLASGVASVLRGKNKPQF
                     TPHVDTGDYVIIVNADKIKVTGKKLEQKIYYNHSDYVGGMKETTLKEMLAKHPERVIE
                     FAVKGMLPKGPLGRQMYKKLFVYAGPDHKHEAQKPETLTF"
     gene            complement(904..1644)
                     /gene="truA"
                     /locus_tag="ACZPD3_04455"
     CDS             complement(904..1644)
                     /gene="truA"
                     /locus_tag="ACZPD3_04455"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR1415289.1"
                     /translation="MKRIGLVVAYDGTNYCGWQTQPNGITVQGVLNDTLSELLGEKIE
                     TIGASRTDAGVHAMGNVAVFDTNTRIPGEKISYALNQRLPEDIRIQLSEEVEPDFHPR
                     YCDSEKTYEYRILNRKFPVPTERLYTYFYHYKLDVDKMKAATSYLIGQHDFASFCGAK
                     AQVKTTIRTVTGIDVWRDGDIVTIRVTGTGFLYNMVRIISGTLIEVGNGQYPPERVKT
                     ILEACNREMAGPTAPAQGLTLMGIEFFD"
     gene            complement(1763..2566)
                     /locus_tag="ACZPD3_04460"
     CDS             complement(1763..2566)
                     /locus_tag="ACZPD3_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR1415290.1"
                     /translation="MIRDITIGQYYPADSVLHKMDPRAKLVGTLVFIISVFVFHTFPG
                     YAVATIFLAAMIILSKVPVKFMFKGLKAIVMLLMITVVFNIFLTPGKVLWQWGILHVT
                     EEGLKLAGRMAIRLTYLVIGSSLMTLTTTPNQLTDGLERLLRPLNKLHVPIHEIAMMM
                     SIALRFIPILMEETDKIMKAQIARGADFETGNIIQKAKNMIPLLVPLFISAFRRANDL
                     AMAMEARCYHGGDNRTQMKPLRYESRDYISYVVMWAYLGIAIVFRIVGI"
     gene            complement(2794..3639)
                     /locus_tag="ACZPD3_04465"
                     /pseudo
     CDS             complement(2794..3639)
                     /locus_tag="ACZPD3_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
     gene            complement(3630..4475)
                     /locus_tag="ACZPD3_04470"
     CDS             complement(3630..4475)
                     /locus_tag="ACZPD3_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR1415291.1"
                     /translation="MGIIKAFKLGFDYLKYDEDGNVQDTQRAVNDVNLDIEAGQFVAV
                     LGHNGSGKSTLAKHLNALLLPTEGTLWVDGIDTSKEPELWKVRQKAGMVFQNPDNQII
                     GTVVEEDVGFGPENMGVPTEKIWERVDESLKKTGMTSYRYHSPNKLSGGQKQRVAIAG
                     VMAMRPKCIILDEPTAMLDPNGRKEVLEAVSDLNRREGVTVILITHYMEEVVHADKVY
                     VMDNGEVVMQGTPREIFSQVETLKEYRLDVPQVTLLAHELHKAGVDVSEGILTTEELV
                     NALCQ"
     gene            complement(4563..>5046)
                     /gene="yihA"
                     /locus_tag="ACZPD3_04475"
     CDS             complement(4563..>5046)
                     /gene="yihA"
                     /locus_tag="ACZPD3_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR1415292.1"
                     /translation="GLMNRKSLARTSAQPGKTQTINFYNINEAMYLVDLPGYGYAKVS
                     QSEKEKWGKMIERYLHTSKNLKAVFLLIDIRHDPSANDKMMYDWILNNGYEPIIIATK
                     LDKLKRSQVQKNIKAIKEGLKLSKDGIIIPFSAETKQGRDEIWALIDELTGLAATEEA
                     "
BASE COUNT         1239 a         1232 c          951 g         1624 t
ORIGIN      
        1 tctaatccta aatattcatc aatgtctctt ttattgattg tgattttacc tgtacccggt
       61 actaaatata ctctggcaac agatgatttt cttcttcctg ttccgtaata ctttgtatta
      121 gccaatgtaa tttacctcct tatcaccgat taaaatgtta atgtctcagg tttctgagcc
      181 tcatgtttgt ggtccggacc tgcatatacg aataatttct tgtacatctg tcttcctaaa
      241 ggtccttttg gaagcattcc ttttactgcg aattcgataa ctctttcagg atgctttgct
      301 aacatctctt ttaatgtagt ctctttcata ccacctacat aatcggagtg attgtagtag
      361 attttctgct ctaatttctt acctgtaact ttgatcttgt cagcgtttac gatgattaca
      421 taatcacctg tgtctacgtg tggtgtaaac tgtggtttat tttttcctct taaaacgctt
      481 gctactccgg atgccagacg gccaagagta catccatcag cgtcaactac ataccatttt
      541 ctttcgattt tatctggatt agccatataa gtctgcatgg ttttacctcc tgttatttat
      601 caacgatagt ttaattaacc gcggaatttc ccgcgaatat aagttattgg aaacgccggt
      661 gctaaaaatg cggaaatgtc cgaaaccgct gataaatact ttcgcctggg ctatttggct
      721 taagcatctc gcactacgtc acatttggtt attatattat aagggttctg ttctgtcaat
      781 aggttttgtg tcgttttttc aatttttttc gttactcccc cgttttttgt tttcagcaaa
      841 aaaactgttg atcagttatc ccaccggcaa ttatcgaaac ggatgatttt ctcctcctga
      901 ctatcaatca aaaaattcaa tccccatcag tgtcagtccc tgtgccggag cagttgggcc
      961 tgccatttcg cggttgcagg cttccagaat tgtctttact ctctcaggcg gatactgtcc
     1021 gtttcctact tcgatcagag ttccggagat gattcgtacc atgttgtaga ggaatcctgt
     1081 tcctgttaca cgtatggtta cgatatctcc gtcacgccat acatcaattc ctgtcactgt
     1141 acggatagtt gtttttacct gcgcttttgc accgcagaaa gaagcaaaat catgctgtcc
     1201 gatcaggtat gaggttgctg ctttcatctt atctacatcc agtttgtagt ggtagaaata
     1261 ggtgtataag cgctctgtcg gaaccgggaa tttgcgattc aggatgcggt attcgtatgt
     1321 tttctcgctg tcgcaataac ggggatggaa atccggttct acttcttcag agagctgaat
     1381 gcggatatct tccggaagac gctggttcag tgcataggaa atcttttccc cgggaatgcg
     1441 tgtgttggtg tcaaatactg ccacgtttcc catggcatga acccctgcgt ctgtacggct
     1501 tgcaccgatc gtttctatct tctcgccaag aagctcggac aatgtatcat ttaatacccc
     1561 ctgtacagta atcccgtttg gctgtgtctg ccagccacag tagttggtgc cgtcatatgc
     1621 aactacaaga ccaattcttt tcatctgttt tcctccataa aaattgaaaa cttttgttat
     1681 aacaaaataa acaatatgct gtaatccttt cctaccggat tacagcatat aaaatatctt
     1741 tataaattat atcagtactt tctcagattc ccacaattct gaaaacaatg gcgattccca
     1801 gataagccca catcaccaca taggaaatgt agtctctgga ctcataacgc aatggcttca
     1861 tctgggttct gttatcccct ccatgatagc accttgcttc catggccatg gccagatcat
     1921 ttgctcttct gaacgcagaa ataaacagcg gaaccagaag cggaatcata ttttttgcct
     1981 tctgaattat atttccggtt tcaaaatctg cgccacgtgc aatctgtgct ttcatgatct
     2041 tatcggtttc ctccataagg attgggataa aacgcagcgc aatagacatc atcatggcaa
     2101 tctcgtggat tggcacatga agtttattta acggcctgag aagtcgttcc agaccatctg
     2161 taagctgatt gggtgtggta gtcagcgtca taagagaaga accgatcacc agatacgtca
     2221 ggcggatggc cattcttccg gccagcttca ggccctcctc tgtcacatgc aggattcccc
     2281 actgccagag aacctttccg ggtgtcagaa atatattaaa taccactgtg atcataagaa
     2341 gcatcacgat tgccttcagt cccttgaaca taaatttcac aggaactttg gaaagaatga
     2401 tcatcgctgc cagaaatata gttgccactg catatcccgg aaatgtatgg aatacaaaga
     2461 cagagatgat gaacaccagc gtacccacca gctttgctct cggatccatt ttgtgcagaa
     2521 cggaatctgc aggataatac tgtcctattg taatgtctcg gatcatactt cctgccctcc
     2581 ctcattctct tcttgttctg cagacttttt atccattgtt tccttctgaa tgcttaaatc
     2641 ttcatcaggc acatctgaat gagactcttt cgtcccatcc ttagtcagat cagcttccat
     2701 ctcactaatt aaggtttctg ctttggcatt ttcttttttc gactgactgt ctgaaacaga
     2761 ttgtctgtta ccggacaatt tcccgagatt acattttctt aaaactgcaa ggatattctc
     2821 ttttgcctca tccaccgttg tagcagttac atctacatcc aggccttttt ccttcaacgc
     2881 atgcatgata taagtaatct gcggtgctgc aagtcccatg gcttccagct ctctgtaatg
     2941 agcaaatact gctttgggag catcgtcata tgcgatttct ccgtcattga ctacgatcag
     3001 tctctccgca tattttgcaa tatcctccat gctgtgggat acaaggataa tggtaatccc
     3061 tcttacttta tgaagctctg cgatctggtc aaggatatca tctcgtcctt tcggatccag
     3121 acctgcagtt ggctcatcca aaataagaat tttcggattc attgccagta caccggcaat
     3181 agcaactctt ttcttctgtc cgccggaaag ctcaaaaggg gatttactga aatttttctc
     3241 cttgaaacct acgtgctgca gtgctttctt tgcttctact tctgcctctt ccctggataa
     3301 tccctgattc atgggaccaa agcatacatc agaaagcaca tcattctcaa agagctgatg
     3361 ctccggatac tgaaatacaa gaccaacctc gcttcgcagg gcacgacgat catatttctc
     3421 tccccagata tcctctccat tataagtgat cgtaccggaa gttggacgga tcagcgcatt
     3481 gaaatgctgg atcagtgtag atttcccact tcctgtatgt ccgataattc cgataaactg
     3541 tccgtccgga atagaaagat tgatatcttt cagcgcatgg atctcatagg cagttcccgg
     3601 actgtaggta taagttacat tttttaattc tattgacata aggcattcac cagctcctct
     3661 gtagtcagta ttccctccga aacatccact ccggctttat gaagttcgtg tgccagaagt
     3721 gttacctgcg gcacatccag tctgtattct ttcagagttt ccacctgtga gaaaatctct
     3781 ctgggtgttc cctgcatcac cacttctccg ttgtccataa catatacctt gtccgcatga
     3841 acaacttctt ccatatagtg agtgatcaga atgactgtca ctccttcccg tctgttaagg
     3901 tcactgactg cctcaagaac ttcctttcgt ccattcggat ccagcattgc agttggctca
     3961 tccaaaatga tgcatttcgg acgcattgcc atgacaccgg caattgcaac tctctgcttc
     4021 tgtccgccgg aaagtttatt cggagaatga taacgatagg atgtcattcc tgtttttttc
     4081 aggctctcat ctacacgctc ccagattttc tctgtgggca cacccatatt ctccggtccg
     4141 aaccctacgt cttcttctac tacagttcct atgatctgat tgtcaggatt ctgaaaaacc
     4201 atgcccgctt tctgtcggac tttccataat tccggctctt tagacgtgtc aatcccatct
     4261 acccacaaag ttccttccgt cggaagcaac agcgcattca ggtgctttgc aagagtagat
     4321 tttccggaac cattatgtcc cagaacagcc acgaattgtc cggcttcaat gtccaggttt
     4381 acatcattga cagcacgctg cgtatcctgc acgttaccat cttcatcata cttgagatag
     4441 tcaaatccca gcttgaatgc cttgataatt cccataatat tttttgtcct cgtattttgt
     4501 ctgtctgctg cttattttca caggaaaaga atcccgtcag taaacagcga atatttattt
     4561 tttcaggctt cttcagtagc tgccagtccg gtcaactcgt caatcagagc ccagatctca
     4621 tctcttccct gcttcgtctc agcagagaaa ggaataataa tgccatcttt gctcagcttc
     4681 agcccttctt tgattgcttt gatatttttc tgaacctggc ttcgtttgag cttgtcaagc
     4741 ttcgttgcaa taatgatcgg ctcatagcca ttgttcagaa tccagtcata catcatcttg
     4801 tcatttgcag atggatcatg acggatatca ataagaagga aaaccgcctt caggtttttg
     4861 gatgtatgaa gatatctttc gatcatcttg ccccattttt ccttctcaga ctgtgaaacc
     4921 tttgcgtatc cgtaaccagg gaggtctacc agatacatgg cttcattgat attataaaag
     4981 ttaatggtct gtgtttttcc cggctgtgca cttgttctgg caagagattt ccggttcatc
     5041 agcccg
//
LOCUS       DBMDXG010000114         5041 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4085_length_5041_cov_1.834008, whole genome shotgun sequence.
ACCESSION   DBMDXG010000114 DBMDXG010000000
VERSION     DBMDXG010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4085_length_5041_cov_1.834008"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(173..1039)
                     /locus_tag="ACZPD3_04480"
     CDS             complement(173..1039)
                     /locus_tag="ACZPD3_04480"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR1415293.1"
                     /translation="MEDNKKKGLGMVLEGGGMRGLYTAGVLDELMEQGIYADSTVGVS
                     AGAIFGCNYKSRQIGRTLRYNTRFCKDKRYMGLKSWITTGDLYSKDFAYGEVPWKLDV
                     FDTETFARSPMKFTVVCTDIETGKPCYQECRMGDRLDVEWMRASASLPLAARPVKLNG
                     RMYLDGGISDPIPVNWMLSQGYEKNVVVCTRHPGYRKEHNKLMPLLRLKFREYPELVK
                     LLDERHIQYNRMLDKIAYLEKEGRIHVIRPDRDISAKPVERDPAHLKEIYEVGRRVMK
                     ADMEKLKAYLAE"
     gene            complement(1048..1857)
                     /gene="thiD"
                     /locus_tag="ACZPD3_04485"
     CDS             complement(1048..1857)
                     /gene="thiD"
                     /locus_tag="ACZPD3_04485"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR1415294.1"
                     /translation="MRTALTIAGSDSSGGAGIQADIKTMITNGVYAMSAITALTAQNT
                     TGVQAILNVTPEFLGQELDSVFQDIYPDAVKIGMVSDKDLIHVIAEKLKQYKAENIVV
                     DPVMVATSGAKLISDDAVEILKQELFPLADVLTPNIPEAEVLVEMSVTNAEEMIEAAG
                     KISETYHCAVLCKGGHSINDANDLLYYDGKYCWFEGKRIDNPNTHGTGCTLSSAIASN
                     LAKGYDLQTAVKRSKQYISGALAAMLDLGKGSGPMDHGFAIRNEYVKESEK"
     gene            complement(1880..2518)
                     /gene="thiE"
                     /locus_tag="ACZPD3_04490"
     CDS             complement(1880..2518)
                     /gene="thiE"
                     /locus_tag="ACZPD3_04490"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR1415295.1"
                     /translation="MKFDKEKMRLYAITDRRWLNGRSLKEVVKESLDGGVTFLQLRDK
                     NSDDETFLQEAAELQELCRDYKVPLIINDNVEIALKMNADGVHVGQSDMEAGAVREKL
                     GPDKILGVSARTVEQALLAQERGADYLGVGAVFATGSKADAAELPHETLKAICEAVSI
                     PVVAIGGITAENISQLKGTGICGVAVISAIYAQNNIKEAAEELKEAVDKIVL"
     gene            complement(2555..2740)
                     /locus_tag="ACZPD3_04495"
     CDS             complement(2555..2740)
                     /locus_tag="ACZPD3_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415296.1"
                     /translation="MDQLQDKRRSNCTVHQEEWLAGKHGRKSANECVKCGKWEEVCPQ
                     HIKIRDELEKVAAELLG"
     gene            complement(2774..4159)
                     /locus_tag="ACZPD3_04500"
     CDS             complement(2774..4159)
                     /locus_tag="ACZPD3_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1415297.1"
                     /translation="MKKENNSAHNHNRGSFSGRIGYVLAVAGSAVGLGNIWRFPYLAA
                     KYGGGIFLLIYILLTASFGYVLIMSETALGRMTRKSPVGAFEHFGKTKSFKIGGWLNA
                     VIPMLIVPYYSTIGGWVIKYLVEYFKGNVQAVAEDGYFGNFISDSWQVELWFLVFAAL
                     VFIIILGGVQNGVERMSKIMMPVLVVLAVVVTIYSVTRPGAVEGVKYFLIPNVKNFSW
                     MTVVAAMGQMFYSLSIAMGILYTYGSYVRKDMDIERSTTQVEIFDTGIAILAGLMIIP
                     AVFAFSGGNPETLQAGPSLMFITLPKVFASMGFGTATGIVFFILVLLAALTSAVSLME
                     TSVSTFMDELHWSRKKCCGLMVVIMIVLGTASSMGYGLLDFIRIFGMNFLDFFDFMTN
                     SVMMPLAALATCILILRVVGIDGMVKEIEQSSPFRRKKLYRVFIKYFAPICLIIILLS
                     SIANVLGIISM"
     gene            complement(4973..>5041)
                     /locus_tag="ACZPD3_04505"
     CDS             complement(4973..>5041)
                     /locus_tag="ACZPD3_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1415298.1"
                     /translation="GVDPSRVRDSIRRGLKQLVKYF"
BASE COUNT         1348 a         1276 c          875 g         1542 t
ORIGIN      
        1 ttggtttatg taccagtttt atacaatttt tattcctgtc tgtttttact aacttcgttg
       61 tatctgacag ctttactgat aacttgctgc tgttcataat tcctcaccca aagtcaattg
      121 caatcagatg gaaatctatc accggattcg ctacctgctg atagactaaa cattactctg
      181 ccaaatacgc cttcagtttc tccatatcag ctttcatcac tctccgtccc acctcataaa
      241 tctcctttaa atgggcggga tcacgctcaa ccggtttggc actgatgtct ctgtccgggc
      301 ggataacatg gatcctgccc tctttttcca gatatgcgat tttatccagc atacgattat
      361 actgaatatg acgttcatcc agaagcttta caagctccgg atactccctg aacttcagtc
      421 tgagcagtgg catcagctta ttatgttctt ttctgtatcc cggatgacgg gtacagacta
      481 ccacattttt ctcatacccc tgtgaaagca tccagtttac aggtatcgga tctgagatgc
      541 ctccgtccag atacattctt ccattcagct ttaccggtct ggcggccagt ggcagggaag
      601 ctgatgcgcg catccattcc acatccagcc tgtctcccat tctgcattcc tgatagcagg
      661 gttttcctgt ttcaatatct gtacatacaa ccgtaaactt catgggtgat ctggcaaatg
      721 tctctgtgtc aaaaacatcc agcttccagg gcacctcccc atatgcaaaa tctttgctgt
      781 aaagatcgcc ggtagtgatc cagcttttca atcccatata tcgtttatcc ttgcagaatc
      841 tggtattata acgaagggtt cgtccaatct ggcgggactt ataattacat ccaaaaattg
      901 caccggcgga aacgccaact gtagaatctg cataaatccc ctgctccatc agctcatcca
      961 gaactccggc tgtatacagt ccccgcatac ctccaccttc cagaaccatt ccgaggcctt
     1021 tctttttgtt gtcttccata gctattctca cttttcactt tctttcacat attcatttcg
     1081 gattgcaaat ccatgatcca tagggccact tccctttccc aggtcaagca ttgccgccag
     1141 tgctcctgaa atatactgtt tggaacgttt tacagcagtc tgcaggtcat atccctttgc
     1201 aagattggaa gcaatggcac tgctcaacgt acagcctgtt ccatgtgtgt ttggattatc
     1261 gatacgcttt ccttcaaacc agcaatattt tccatcatag taaagcagat catttgcatc
     1321 attaatgctg tgtccgcctt tgcaaagtac tgcacaatga tatgtctcag aaatctttcc
     1381 ggcagcctct atcatttcct ctgcattggt cactgacatt tcaaccagaa cctctgcctc
     1441 cggaatattc ggagtaagca catcagccag tggaaacagt tcctgcttca atatctccac
     1501 tgcatcatca ctgatcagct tggcaccgct tgttgcaacc ataacaggat ccacaacaat
     1561 gttttccgct ttatattgtt tcagtttctc tgctattaca tgaatcagat ctttatctga
     1621 taccattccg atttttaccg catccggata aatatcctga aaaacagaat ccagttcctg
     1681 ccccagaaat tccggtgtta catttaatat tgcctgtaca ccggttgtat tctgtgcagt
     1741 taatgcggta atggcactca ttgcatatac accatttgtg atcattgtct taatatctgc
     1801 ctggattccg gcgcctccgc tggaatcact tcctgcgatc gtcagtgcag ttctcataat
     1861 tgtcttcctc tcatttcttc tataatacaa tcttatctac tgcctctttc agttcttctg
     1921 ctgcttcttt aatgttattt tgagcataaa tggcactgat cactgccaca ccgcaaatcc
     1981 ctgttccctt tagctgagat atattttctg ctgtgatccc tccgattgcc accacaggga
     2041 tagataccgc ttcacagatt gccttcagtg tttcatgggg aagttctgcc gcatctgctt
     2101 ttgaaccggt tgcaaatact gcacccaccc ccagataatc tgcaccacgc tcctgtgcaa
     2161 gcaatgcctg ctctaccgtt cgtgccgaaa ctcccaggat tttatccggt ccaagtttct
     2221 cacgcacagc acctgcttcc atatcactct gccctacatg aacaccatct gcattcatct
     2281 tcaaagcaat ttccacattg tcatttatga tcagaggtac cttataatct ctgcataatt
     2341 cctgaagttc agctgcttcc tgcaggaaag tttcatcatc tgaattttta tctctgagct
     2401 gcagaaaggt tacgcctcca tccagactct cctttaccac ttccttaagg ctccgcccat
     2461 taagccagcg acggtccgtt attgcgtata agcgcatttt ctctttatcg aacttcatgt
     2521 ttcactcctc ttttctactc ttatctatag caatttatcc caaaagttct gctgcaactt
     2581 tctccagctc atctctgatc ttaatatgct gcggacatac ttcttcccac ttaccacatt
     2641 tcacacattc attggcagat ttcctgccat gtttgcctgc cagccattct tcctggtgaa
     2701 ctgtgcagtt gcttcttctc ttgtcttgca gttgatccat gctgcattct ttgtaaaaag
     2761 agtcatatat tttttacata ctgataattc ccaaaacgtt tgcaatagaa ctcagcaaaa
     2821 taatgatcag gcaaatcggt gcaaaatatt tgataaatac tctgtacagt ttctttctgc
     2881 ggaacggaga agactgttca atttctttta ccattccatc aataccaaca acacgaagaa
     2941 tcagaataca ggttgcaagt gctgccaacg gcatcattac tgagtttgtc ataaaatcga
     3001 agaaatcaag gaagttcatt ccaaagatac gaataaaatc cagaagtcca tatcccatgg
     3061 aagatgcggt tccaagtaca atcatgatca caaccatcag accgcagcat ttcttacggc
     3121 tccagtgaag ttcgtccatg aatgtggaaa cactggtttc catcagggaa actgcacttg
     3181 tcagagctgc aagtaataca agtatgaaga atacgattcc tgttgcagta ccaaatccca
     3241 tacttgcaaa tacttttggc aatgtgataa acatcagaga tggtcctgcc tgaagtgttt
     3301 caggatttcc accggagaat gcaaataccg ccggaataat catcagtccc gcaaggattg
     3361 cgatacctgt atcgaaaatc tcaacctgtg tggtagatct ctcaatatcc atatctttac
     3421 ggacatagga accgtaagta tagaggattc ccattgcaat ggacaaagaa taaaacatct
     3481 gtcccatcgc tgctactact gtcatccagg agaaattttt cacattcggg atcaggaaat
     3541 atttcacacc ttcaacagca cccggacgtg tgacagaata gattgtaact acgactgcca
     3601 gaactaccag aaccggcatc atgatctttg acattctctc tacaccgttc tgtacaccac
     3661 cgagaatgat gatgaataca agcgccgcaa ataccagaaa ccagagttcc acctgccagg
     3721 aatcggaaat aaagtttcca aaatatccgt cttccgctac tgcctggaca tttcctttga
     3781 aatattccac aagatactta attacccatc cgccaatcgt actgtaataa ggaacgatca
     3841 gcattggaat cactgcattt aaccatccgc cgattttgaa tgatttcgtt tttccaaaat
     3901 gctcaaatgc acctaccgga ctctttctgg tcattcgtcc aagggctgtt tctgacatga
     3961 tcagtacata accaaatgat gcagtcagaa gaatatagat cagcaggaaa attccgcctc
     4021 catatttcgc tgcaagatac ggaaatctcc agatatttcc cagtcctact gcagaacctg
     4081 caactgccag cacataacca atccttccgg aaaagcttcc tctgttatgg ttatgtgcag
     4141 aattgttttc ttttttcatt atacacctca cctcgttaca aaaacctccc ataggatttc
     4201 tcccatggga ggtctgctag ttttttactg attctatagt atcaaaaaga tgcgggaatg
     4261 tcaaagacta ataataatta cagacttaat tgtaacatag ttactgttca cacaccacta
     4321 tcccagtata tatttcaagt atagtctttc tgtacacatt gttctatgat ataatagttg
     4381 agctaccacc cctagacagg tgcagaaagg gggtgtgttg taaatggata ttatcttatc
     4441 atttatgctt tctatcttgg caagtgtaat cgcctactac atttgcaaat ggttggatag
     4501 aaaatagcag ctggtagcca gcctggggga ataagccacc ctgcccaaac ggaataaaaa
     4561 agcctcagag gtcgcaactc tggggctttt gctgtgttat aaatgaattt aaccttatca
     4621 ttttgcctac tggcattata tcatatgcat tttttgtttg caatatactc aatatacttt
     4681 ttcgaatcaa gtcaaacttt tggttactgt tcacacatca ctattccgcg aggtgttttg
     4741 gcatgaatct gccacaatcc gttttatgaa atcgcagatc gctccgaagc agttctttca
     4801 tcacctccct gactgctcca tcttcgcggc gtttcacatt cgctcgtaca tgagttatgt
     4861 acctgttgtg ctgtctattc gattgtcaat gtactgatga aggaggaaaa cttgtccttc
     4921 ttattaacac tgacaaaaaa ggcaaaaaac aggcgcatca aatgaaattt tatcaaaaat
     4981 attttacaag ctgctttaat ccacggcgga tactgtctcg gacacggctt gggtctacac
     5041 c
//
LOCUS       DBMDXG010000115         5024 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4097_length_5024_cov_1.705667, whole genome shotgun sequence.
ACCESSION   DBMDXG010000115 DBMDXG010000000
VERSION     DBMDXG010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4097_length_5024_cov_1.705667"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..4563
                     /locus_tag="ACZPD3_04510"
     CDS             <1..4563
                     /locus_tag="ACZPD3_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012873.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HIR1415299.1"
                     /translation="FIKETKPAYMVPAVTMQIDKIPLNQNQKVNKKALLLPEKKAAEI
                     IKPENEVQQILFDCIAEVLGYTDFGITTDIYEAGLTSITAIKLNILISKAFDIVIKTS
                     DIKNHPTIRMMEEFVKTAGKESKREVQESYPLTNTQEGIFIECTANMGSTIYNIPYLL
                     KLDNKVDLDRLAEAIDSTVEAHPYLKTRLFMDDNGNVLQKRNDGLSYKTPILNGMNRD
                     TLVRPYMLFNEQLFRFEIYRTCDGNYLFLDLHHIVADGTSLAIIINDINRAYSGEKLE
                     PEGYTSYDLALDNRDALAGDIYKNAENYYKSVFEHAGGSISFYPDKNGAAPTAELYHR
                     ETRSISVQDVKEFCKKHGITENVFFISAFGITLGKYNFKKDAVFTTIYHGRNDSRLSE
                     TVGMLVKTLPVFCDFSGTAKDCLSGVQKQLIDSMNNDIYPFSQISHEFDIKADAMVIY
                     QGDNFAFDTIGGEYAQEEPVSLNMAKAPVSVSISIEKNRFVFEIEYRGDMYHEETIRY
                     LADNLETTAEGILRECDPADIRLMFEEETQMEDIPEHAGKTFIDLFKEMAARYPDRPA
                     VRDDSGDFTYRELDRMSDYIAQKLTENGFGPEQAAGILCGRTKEYTVAYVGVMKAGGA
                     YVPLDPEYPQSRIEYMLKDSGARNLLVIDQYQNLVEFYDGNVISLDSVSDEAEDFELS
                     AELISPKPENLAYMIYTSGSTGKPKGVMIEHRNLLNLIEYITLSRNTSPDDIVAEFAS
                     FCFDASVIDLFAPLTAGAVLYILPESIRKDAIAISRYIKEKEITTVTFPTQMGELVTE
                     LLEDAPALKFVTLGGEKFKHYRNRTYQMINGYGPTENTVSSTEFLVDRQYDNIPIGKS
                     QRNVRSYIVDENLNRLPVGASGELCHAGRQIARGYHNLPEKTASVFVENPFAVCEQES
                     RLYRTGDMVRMKGDGNIEYIGRIDSQVKIRGYRVELGEIEGALLKHELVKNAAVTVIE
                     KGGNKYITAYYTGEAIPEDELKTFLEPLIPDYMMPSFFVSIEEMPVTPGGKIDKKALP
                     MPEVTTNTASYVEPVTAAQRALCEIFEKALGIERVGIEDNFFELGGSSLTASKVAVMC
                     LSKNISLVYADIFKYPTVRELAALVDDDGVTEAAQSKNEFSDYNYNRIQNVISANTEE
                     NADRITKEKLGDIMVTGATGFLGIHVLKAFLDNYDGKVYCLVRKGKYESPEKRMMNML
                     MYYFDDPYKELFESRIICVDGDITSKEQVTGFSEYKFSTIINCAACVKHFAADDVLER
                     INVQGVENLIDFCKNNGRRLIQISTVSVAGEGSDGVPPMSRVFCENDLYIGQNITNEY
                     IRTKFLAERAVLEAVSEGLDGKVVRVGNLISRNSDGEFQINFITNGFLRSLRGYAAVG
                     KFPMGGMHEVAEFSPIDSTALAVLRLAQTDRRFTVFHACNSHHIYMADLIYAMRSYGF
                     RIDIVRDEEFEAAVKEFAKTGQDSDAVSGLIAYTSHNENEIYTIDYSNRFTAQVLYRL
                     DYKWPVTDDRYLENAIAALDRLTFFD"
     gene            4583..4885
                     /locus_tag="ACZPD3_04515"
     CDS             4583..4885
                     /locus_tag="ACZPD3_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR1415300.1"
                     /translation="MEIITKTEGNKATMEISGWLDTQTAPQLGEALSGLEDSITSLVF
                     DFTNLEYISSAGLRQVIVAYKKMAQKDGFKIINVSDEVYDVFSLTGFDSKIDIQKK"
BASE COUNT         1515 a          961 c         1239 g         1309 t
ORIGIN      
        1 tttattaaag agacaaagcc tgcctatatg gtacctgcag ttaccatgca gattgacaaa
       61 attcctctga accagaacca gaaggtaaat aaaaaggctc ttcttctgcc tgagaaaaaa
      121 gcagcggaga tcattaagcc tgagaatgag gttcagcaga tattatttga ctgtatcgca
      181 gaggttctgg ggtatacgga ttttgggatt accacagata tttatgaggc aggtctgaca
      241 tccatcactg ccattaagct gaatatcctg atctccaaag cctttgacat tgtgatcaaa
      301 acttcagata ttaagaatca tcccactatc cgaatgatgg aggaatttgt aaaaactgcg
      361 ggaaaagaaa gcaaaagaga agtacaggaa agttatcctc ttaccaacac tcaggagggg
      421 attttcattg agtgtactgc aaatatgggt tctaccattt acaatatccc gtatctgtta
      481 aagcttgata acaaggtgga tctggacaga ctggcagaag ccattgacag tactgttgag
      541 gcgcatcctt atctgaaaac ccgtctgttt atggatgaca acggaaatgt acttcagaaa
      601 cgaaacgatg ggctcagtta taaaacaccg attttaaacg gaatgaacag agacactctg
      661 gtacgtccat atatgttgtt taatgaacag cttttccggt ttgaaatcta cagaacctgt
      721 gatggcaatt atctgttcct ggaccttcac cacattgttg ccgatggtac atctctggct
      781 attataatta atgatattaa cagagcttac agcggtgaga agctggaacc ggaaggttat
      841 acttcctatg atctggcact ggataacaga gatgccctgg caggtgatat ctataaaaat
      901 gcagagaatt attataagtc agtatttgaa catgcaggtg gaagcatcag cttctatccg
      961 gacaaaaacg gtgcggctcc aacagcagaa ctttaccaca gagagaccag atccatttcc
     1021 gtgcaggatg tgaaggaatt ctgtaaaaag catggaatta cggagaatgt attcttcatt
     1081 tccgcttttg gtatcacact tggaaaatat aatttcaaaa aggatgctgt atttaccact
     1141 atttaccatg gaagaaacga ttccagactt tcggaaacag tgggtatgct ggtaaaaaca
     1201 ctccctgttt tctgtgattt ctcaggaaca gccaaagatt gcctgagcgg tgtacagaaa
     1261 cagctgatcg attccatgaa caacgatatt tatcctttct cacagatcag ccatgagttt
     1321 gatattaagg cagatgccat ggttatttac cagggcgaca attttgcttt tgacaccatt
     1381 ggtggggaat atgcacagga ggagcctgtc agcctgaaca tggcaaaggc accggtttct
     1441 gtcagcattt ccattgaaaa gaacagattt gtctttgaga ttgagtacag aggggatatg
     1501 taccatgagg aaactatccg gtatctggct gacaatctgg aaaccactgc agagggaatc
     1561 ttaagggaat gcgatccggc tgatatccgg ctgatgtttg aggaagaaac tcagatggag
     1621 gatattccgg aacatgcagg aaagaccttc atagatctgt ttaaggagat ggctgccaga
     1681 tatccggaca gaccggcagt gcgggatgac tccggagatt ttacttacag agagttggac
     1741 agaatgtctg attatattgc tcagaagctt acggaaaacg gatttggccc ggaacaggca
     1801 gcaggtatcc tgtgcggcag gacaaaggaa tatacagtag cctatgtggg tgttatgaag
     1861 gccggcggag cctatgttcc tctggatccg gagtatccac agagcagaat tgaatatatg
     1921 ctgaaggatt caggagcaag aaatctcctt gtgatcgatc agtatcagaa tcttgtggaa
     1981 ttttatgacg gaaatgtgat cagtctggac agtgtgtcag atgaggctga ggattttgaa
     2041 ctttctgcag agcttatttc tccgaaaccg gagaaccttg cctatatgat ctatacctca
     2101 ggctccacag gaaagccaaa gggtgttatg attgagcaca gaaatctgtt gaacctgatc
     2161 gaatatatca cactgagcag aaatacttca ccggatgaca ttgtggcaga gtttgcgagc
     2221 ttctgtttcg atgcgtcagt gatcgatctg tttgcacctc ttaccgcagg agcagtcctg
     2281 tatattttac cggagagtat ccgtaaggac gccattgcca taagcagata tataaaggaa
     2341 aaagagatca ccaccgtaac cttccctacc cagatgggag aactggttac agaacttctg
     2401 gaggatgcac ctgctcttaa atttgttaca cttggcggcg agaaattcaa gcattaccgt
     2461 aaccgtacct atcagatgat caatggctac ggtcctacag agaatacggt ttcttccaca
     2521 gagtttttgg tagacagaca gtatgacaac atcccgatcg gaaaatcaca gcggaatgtg
     2581 cgcagctaca ttgtggatga gaatctgaac cggcttccgg tgggtgcgtc cggcgaactt
     2641 tgtcacgcag gaaggcagat tgccagagga tatcataatc ttccggaaaa aacagcatct
     2701 gtgtttgtag aaaatccatt tgctgtatgt gagcaggaaa gccgtcttta caggaccggt
     2761 gatatggtcc gcatgaaagg cgacggaaat attgagtaca taggccggat cgattcacag
     2821 gtgaagatca gaggatatcg tgtggagctt ggtgagatcg aaggtgccct tctgaaacat
     2881 gagctggtga aaaatgcagc cgttacagta attgaaaagg gtggaaataa atatatcaca
     2941 gcttattata caggagaagc catcccggag gatgaactga agaccttcct ggagccgctg
     3001 atcccggatt atatgatgcc gtctttcttt gtcagcattg aggaaatgcc tgtgacaccg
     3061 ggcggtaaga ttgataaaaa agcactgcca atgccggagg taaccacgaa cactgccagc
     3121 tatgtagagc cagttacagc agctcagaga gcactctgtg agatttttga gaaagcactg
     3181 ggaattgaaa gagtagggat tgaggacaac ttctttgaac tgggcggatc ttcccttact
     3241 gcatccaagg ttgctgttat gtgtctgtca aagaatattt cactggtata tgcggatatt
     3301 ttcaaatatc ctactgtacg ggaacttgca gcgctggttg atgatgacgg agttacagag
     3361 gctgcacaga gcaagaatga attttcggat tataactata acaggatcca gaatgtgatc
     3421 agtgcaaata cagaagaaaa tgcagaccgg atcacaaagg aaaagctggg agatattatg
     3481 gtcacaggtg ccacaggttt cctggggatc catgtcctta aagcattcct tgacaattat
     3541 gacggtaagg tttactgtct tgtacgtaag ggcaaatatg aatcgcctga aaagcgaatg
     3601 atgaacatgc tgatgtacta ttttgatgat ccatacaaag agctgtttga aagccgtatt
     3661 atctgtgtgg atggggatat tacctcaaag gaacaggtaa caggattttc agaatacaaa
     3721 ttcagcacca tcattaactg tgctgcctgc gtaaagcatt ttgcggctga tgatgtactg
     3781 gaaagaatta atgtgcaggg tgtggaaaat ctgatcgatt tctgtaagaa taacggccgc
     3841 agactgatcc agatttctac ggtttccgtt gcgggagagg gttctgacgg tgttccgcca
     3901 atgtccagag ttttctgtga aaatgatctg tacatcggac agaatattac caacgaatat
     3961 atccgtacca aattcctggc agagagggcg gttctggaag cagtttccga gggactggac
     4021 ggcaaggtag tccgggtagg taatctgata agccgaaact ctgacggcga gttccagatt
     4081 aatttcatta ccaatggatt ccttcgcagc ctgagaggtt atgcggctgt aggtaaattc
     4141 cctatgggcg gtatgcatga ggtggcagaa ttctcaccga ttgattctac tgcgctggca
     4201 gttctgagac tggctcagac cgaccggaga tttactgtat tccatgcatg caacagccat
     4261 catatttata tggcagacct gatctacgca atgcgcagct atggattcag gattgatatt
     4321 gtaagagatg aggagtttga ggcagcagtg aaggaatttg caaagaccgg tcaggattct
     4381 gatgcggttt ccggactgat cgcctatacc tcacataatg aaaatgaaat ctataccatt
     4441 gattacagca atcgttttac agcacaggtt ctgtacaggc tggattataa atggccggtt
     4501 acagatgaca gatatctgga aaatgcaatt gcagcacttg acagacttac attttttgat
     4561 tgattcagga gggaaaatta caatggaaat tatcacaaaa acagaaggta acaaagcaac
     4621 tatggaaatc agcggatggc ttgatacaca gacagctccc cagttagggg aggctctgtc
     4681 cggacttgaa gacagcatca caagcctggt atttgatttt acaaatctgg agtatatttc
     4741 atctgcaggc ttacgtcagg tgatcgtagc ctacaaaaaa atggctcaaa aggatggatt
     4801 taagatcatc aatgtttctg atgaggttta cgatgtattc agccttacag ggtttgacag
     4861 caagatcgat attcagaaga agtaactgtt gatcagatgc agaaattata caggatcaat
     4921 attaatcatt ttgaggatcc gcttaagaat gaaaggcttc tggatctggt tgggatcaga
     4981 agaagagaaa aggtcatcag gtatcgtatg cctgatgacc gcaa
//
LOCUS       DBMDXG010000116         5004 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4113_length_5004_cov_1.630226, whole genome shotgun sequence.
ACCESSION   DBMDXG010000116 DBMDXG010000000
VERSION     DBMDXG010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4113_length_5004_cov_1.630226"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            285..1550
                     /locus_tag="ACZPD3_04520"
     CDS             285..1550
                     /locus_tag="ACZPD3_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1415301.1"
                     /translation="MSQQTITLLFLVFTIISFILEKIPLGLTASICAIGLTLTGVVDV
                     STTFSQYVNSNVILCVGMFVVGQALFETGMANKIGGLVTRFARTERTLIIAIMVIVGV
                     MSGFLSNTGTAAVLIPVVCGIADESGYSRSRLLMPLVFAAALGGNLSIIGAPGNLMGV
                     NALQEMGLSTSFFMYAPVGVPMLLVGIIYFVFIGYRFLPDGKGAVGATVEKQKDFSDV
                     PKWKQTVSLIVLILVILAMIFEKQIGVSIEISACIGAIILVLVGVLSEKEALASIDLK
                     VVFLFGGSLTLAKALDTTGAGELIADKIVGLLGADPNPIVLLLVIFIVTCALTNFMSN
                     TATTALMIPIAVSLANNLGADPRAVVIATVIAGSCAYATPIGMPANTMVVGLGGYKFK
                     DYVKSGLPLILVSFVICMILLPVLFPFYP"
     gene            1696..2601
                     /gene="ttdA"
                     /locus_tag="ACZPD3_04525"
     CDS             1696..2601
                     /gene="ttdA"
                     /locus_tag="ACZPD3_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HIR1415302.1"
                     /translation="MTKENGVKVLTDMVADFVAHIGKKLPDDVVTKLEELGSQESAAL
                     PKVLYETMTKNQGLAVELNRPSCQDTGVLQFWLKCGTNFPYINELEALLKEAVVQATF
                     AAPLRHNSVETFDEYNTKKNVGKGTPTVWWDIVPNSDKCEIYAYMAGGGCTLPGKAMV
                     LMPGAGYEGVTDFVLDQMTTYGLNACPPLLVGVGVGTSIETAALNSKKALMRPIGSHN
                     DNANAAKMEKLLEDGINAIGLGPQGMGGKYSVMGVNIENTARHPSTIGVAVNVGCWSH
                     RRGHLTVNSDLTVTCDTHSTWKFNA"
     gene            2617..3249
                     /gene="ttdB"
                     /locus_tag="ACZPD3_04530"
     CDS             2617..3249
                     /gene="ttdB"
                     /locus_tag="ACZPD3_04530"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HIR1415303.1"
                     /translation="MIEIRGDKKVLITPISEEDLADIKIGDIVWLDGELMTCRDVAHR
                     RLVEYGRELPYDIKDKAIFHAGPIVRKIEGTEDDYEMVSVGPTTSMRMEKFEYEFTKL
                     TGVRVIVGKGGMGPNTERACKEFGAIHCVFPAGCAVVAATEVEKIVEHHWDELGMPET
                     LWCNKVKEFGPLIVSIDAQGRNLFEENKVIFNERKEAAKQAIYPEVKFIK"
     gene            complement(3570..3659)
                     /locus_tag="ACZPD3_04535"
                     /pseudo
     CDS             complement(3570..3659)
                     /locus_tag="ACZPD3_04535"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            3630..3698
                     /locus_tag="ACZPD3_04540"
                     /pseudo
     CDS             3630..3698
                     /locus_tag="ACZPD3_04540"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3877..>5004
                     /locus_tag="ACZPD3_04545"
     CDS             3877..>5004
                     /locus_tag="ACZPD3_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR1415304.1"
                     /translation="MKKFRLNVQRHICILLCLILYITVLPLPVSAEEAKSKTVRVGWY
                     EGTYNTTGPDGQRRGYSYEYQQAVAAHTGWKYEYVEGSWAELMSMLKNGQIDLLGGIS
                     YTEERSTSMLFSELPMGEDKYYLYVDTSNTDISTSDLTTLNGKRIGMLPNALPAEMFH
                     EWEKSHGVNTQQVDITSVDDVRQKLKDHEIDGFVLNESPQWERDNISPAILIGGSYNY
                     FAVSKKRPDLKEELDQVMQKIERENPFYTDDLYKRYLSANSLETLTDEEQNWLEQHGA
                     VRIGYLKNDVGISLVDTESEKPVGIINDYISLVSGYLGEQAIEFQLTGFESQEKELQA
                     LKDNRIDMIFHMNQNPYEAEQNDIVLSNTVFEINVAVLTGVE"
BASE COUNT         1595 a          921 c         1149 g         1339 t
ORIGIN      
        1 cctatcataa tttccatatt tatttctaaa gtttttttta atgtagtaat attgcataaa
       61 aaaaacctgc taaaattgag attataaaaa ttttttatgg ggatatgcaa cccattaatt
      121 tgttttttta tacgtgcatg atttataata aactcatcaa aggaaacgtg cacggccggt
      181 acctttcaag agagattaat aaaaaaaaga cggataagta agacaaaagc gaagagcaaa
      241 ataaaaaatg aaatcacaga aaaaagtgaa aggagatcaa taaaatgtct caacaaacaa
      301 tcacactgtt atttcttgtg tttacgatca ttagtttcat cctcgaaaag ataccacttg
      361 ggcttacagc ttcgatctgt gcaatcggat tgacactcac cggagtagtg gatgtatcta
      421 caacgttttc acagtatgta aacagtaacg taatcctttg tgtcggcatg tttgtagtag
      481 gtcaggcact atttgaaaca ggaatggcaa ataaaatcgg aggtctggtc accagatttg
      541 ccagaacaga aagaacattg attattgcaa tcatggtaat cgtaggtgtg atgtccggat
      601 tcttatcaaa cacaggtaca gcagcagtgc tgatccctgt agtctgtgga atcgctgatg
      661 aatccggcta ttcaagaagc cgccttttga tgccactggt atttgcagca gcgcttggcg
      721 ggaatctttc catcatcggt gcacccggaa acctgatggg tgtaaacgca cttcaggaaa
      781 tggggctttc cacaagcttt ttcatgtatg caccggtggg agtcccgatg cttctggtag
      841 gaattatcta cttcgttttc attggataca gatttcttcc ggatggaaaa ggggctgtag
      901 gggcaactgt tgaaaaacag aaagatttca gtgatgtacc gaaatggaaa cagactgttt
      961 cactgatagt tctgatactt gtgattcttg caatgatttt tgagaaacag attggagtca
     1021 gcattgagat ttctgcatgc atcggagcaa tcattctggt gctggttgga gttctttcag
     1081 aaaaagaagc actggcttcc attgatctga aagtagtatt cctttttgga ggatctctta
     1141 cacttgccaa agccctggat acaacaggtg ccggtgaact gatagcagat aagatcgtag
     1201 gacttctggg tgcagacccg aacccgatcg tgcttcttct ggtaatattc attgtaacct
     1261 gtgcactgac aaacttcatg tcaaacacgg ccacaaccgc actgatgatc ccaattgcag
     1321 tttctctggc aaacaacctt ggtgcggatc cgagagctgt tgttatcgca actgttatcg
     1381 caggctcctg tgcatatgca acaccgattg gaatgcctgc aaacactatg gtagtaggac
     1441 ttggaggata taaattcaag gattacgtga aatcaggttt accgttgatt ctggtatcct
     1501 tcgtgatctg tatgatcctt ttgcctgttc tgttcccgtt ctatccgtga gaggataaac
     1561 catatgagac ggaagatttt ccgcttaatg taaatgtatc aatagaaata aagcagaaaa
     1621 catgctgctt tagataaata acccaaaatt aaaagccaaa caaccctaaa ccattgaatg
     1681 atcaggaggt aaatcatgac taaggaaaat ggagttaaag tattaacaga catggtagca
     1741 gattttgttg cccatattgg aaaaaaatta ccggacgacg tagtaacaaa actggaagag
     1801 cttggttctc aggaaagcgc agcacttcca aaagtacttt atgagacaat gactaagaac
     1861 cagggactgg ctgtagagct gaaccgccca agctgccagg acacaggagt tcttcagttc
     1921 tggctgaaat gcggaactaa tttcccgtac atcaacgagc tggaagccct tttgaaagaa
     1981 gctgttgtac aggctacttt tgcagcacca ttaagacata actctgtaga aactttcgac
     2041 gagtacaaca ccaagaaaaa cgttggaaaa ggaactccga ctgtatggtg ggacatcgtt
     2101 ccaaacagtg ataaatgtga gatttacgca tacatggcag gaggcggatg tactcttccg
     2161 ggaaaagcaa tggtacttat gccaggtgca ggatatgaag gcgttaccga ttttgttctt
     2221 gatcagatga caacttacgg actgaatgct tgtcctccgc ttcttgtggg tgtaggtgta
     2281 ggaacatcta tagagacagc agccctcaac tccaagaaag cacttatgcg tccgatcggt
     2341 tctcacaatg acaatgcaaa tgcagcaaaa atggagaagc ttctggaaga cggaatcaat
     2401 gcaatcggcc ttggaccgca gggaatgggc ggaaaatatt ctgtaatggg tgttaatatc
     2461 gagaatacag cccgtcatcc gtctacaatc ggtgtagcag taaatgtagg ctgctggtca
     2521 caccgccgtg gtcacctgac cgtaaattct gaccttacag taacctgtga tacacattcc
     2581 acatggaaat tcaacgcata atcggaggga aaaaacatga tcgaaatcag aggagataaa
     2641 aaagttctta ttacaccaat cagtgaagaa gacctggctg acatcaaaat cggtgatatt
     2701 gtatggctgg acggagagct gatgacctgc cgtgatgtgg cacatcgccg tctggttgag
     2761 tatggaagag aacttccata tgatatcaag gacaaagcaa ttttccacgc tggcccaatc
     2821 gttcgtaaaa ttgagggaac agaagatgac tatgaaatgg tttccgttgg accgacaacc
     2881 tccatgcgta tggagaaatt tgagtatgaa tttactaagc ttacaggtgt aagagtaatc
     2941 gttggaaaag gcggaatggg accgaataca gagcgtgcat gcaaagaatt tggtgcgatc
     3001 cactgtgtat tcccggctgg ctgtgcagta gtagctgcaa cagaggttga gaagatcgta
     3061 gaacatcact gggatgagct tggaatgcct gagaccttat ggtgcaacaa ggtaaaagaa
     3121 tttggcccac tgatcgtatc tatcgatgca cagggccgca acctgtttga agaaaacaag
     3181 gttatcttca atgagagaaa agaagctgct aagcaggcta tttatccgga agttaagttt
     3241 attaaataat gcatttcagt tttacaaaag taaaaaccgt acgatttctt ctttattgtt
     3301 tcaaagataa aaaaagataa acatcatcta agatgaaaag aaaaggtggc tcacagattt
     3361 ccttcataca tggtctgtgg gctgcttttt tctgtatacg aaatttaaat aaatagtaac
     3421 tttaaccgaa tcaagtaagt cccctgcgga ggttgcgaaa agcaataagc agtgggtggg
     3481 gactcgcata caaatgatta tgccactgct acatcttttt cctacgcctt gaaataaaaa
     3541 aaaattatcc tgcaagattg tttcaggaat taacatgtcg acacattaga gcgtgtctga
     3601 aaaatcattc ccgcaatctg cacgccccac tttgcgatat attttgtccg aattcggttg
     3661 tcgtagccca tgaattttgg caagtaaaga ttatctgaaa actgaaaaac aggtataaaa
     3721 aataaatata gggctgttgc aatgacggaa aataaggtat gatatctgca gaagcatgat
     3781 acatgtttcc ataacccggg tgaagtaaat gaaaaaggca ataatctgtt gagatatctt
     3841 caccggacag acacaaaatc atttggagaa agttatatga aaaagtttag gttaaatgta
     3901 cagagacata tctgcatttt attatgcctt attttatata ttactgtgct tccccttcct
     3961 gtatcagcag aggaagcaaa atccaaaaca gtcagagtag gctggtatga gggaacctat
     4021 aataccacag gaccagacgg tcagaggaga ggttacagct acgaatacca gcaggcagta
     4081 gctgcgcata ccggatggaa atatgaatat gtggagggaa gctgggcaga actgatgagc
     4141 atgctgaaaa atggacagat cgatctgctg ggaggcattt cctatacaga ggaacgttcc
     4201 acttccatgc ttttttcaga attgcctatg ggagaggata aatattacct gtatgtggat
     4261 acttctaata cagatatctc cacttcagat ctgactacat taaatggaaa aagaatcggt
     4321 atgctgccga atgcacttcc tgccgaaatg ttccatgagt gggagaaaag tcatggagtg
     4381 aacacgcagc aggttgatat taccagtgta gacgatgtcc gtcagaaatt aaaagatcat
     4441 gaaattgacg gatttgtttt aaatgaatct ccacagtggg aaagagacaa tatttctcct
     4501 gctattctga ttggtggctc ctataattat tttgcagtca gcaaaaaacg cccggatctg
     4561 aaagaagagc tggatcaggt aatgcagaag atagagaggg agaatccttt ttatacagac
     4621 gatctttata aaaggtatct ttctgcaaat tcccttgaaa ctcttacgga tgaggagcag
     4681 aactggctgg aacagcatgg ggcggtcagg attggatatc tgaaaaatga tgtgggtatc
     4741 agccttgtgg atacggagtc agagaaaccg gtaggaatta ttaacgatta tattagtctg
     4801 gtatccggtt atctgggtga acaagccata gaatttcaac tgacaggttt tgaatcccag
     4861 gagaaagagc tgcaggcatt aaaggataac aggattgata tgatttttca tatgaaccag
     4921 aatccatatg aagctgagca gaatgatatt gtattgtcaa atactgtttt tgaaattaat
     4981 gttgcagtac tcaccggtgt tgag
//
LOCUS       DBMDXG010000117         4970 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4154_length_4970_cov_2.054837, whole genome shotgun sequence.
ACCESSION   DBMDXG010000117 DBMDXG010000000
VERSION     DBMDXG010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4154_length_4970_cov_2.054837"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="ACZPD3_04550"
     CDS             complement(<1..1189)
                     /locus_tag="ACZPD3_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012544.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1415305.1"
                     /translation="MSSTVKEIKEKTGYLKRSDLYTIGVGQAIGSGVLTLIGPAILLT
                     GQSAWLAYVAACIWGFMMIAPIPWITSTLKLGGGFYSLVGDMAGKRVAGMYAVGFLPQ
                     TINLATYGVAIGMYANSLWPQINAKWFGIAALSVFFAINLCGVDVMAKVQKILVILLF
                     VALGLFIVLGCLQLKNPVFEFTAPDFFSNGPSGFFSAIMLYVYSTNGYSCIMNYGKQA
                     KEAHKDIPKALLYCIPTLMVIYGGVAIVASGVLPLDQVAGQPLTLVAKNILNPALFTV
                     FMIGGPVLALSSSINSTISNNCIPVAQSCKDGWLPKSWAAQNRRGAYWKLMTFTYLMG
                     ILPVLLDFSISDVVNNIMLLASAVAFLQIYAYFQLPKKHAEAWEKSPMHISNGKYYFL
                     CCLS"
     gene            1449..2285
                     /locus_tag="ACZPD3_04555"
     CDS             1449..2285
                     /locus_tag="ACZPD3_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR1415306.1"
                     /translation="MKIEQLIREKVSFLSAGQKKVAEYILQHLDSFSYSTLAKLSKEI
                     SVSETTIIRLAYSLGFESFSEMQRTVQNDILSVPTVIKDDSLLDSGNFYQNIIANELH
                     SMESWASHLNQEDMDQIVTHLSNAKKRLIVGARSSYSAALWMGTLLNQLLGNTKVVHE
                     FYDSNFEYLTEASEDTVVLLISFARYTKWSLKYAQIAKNHGAKILAITDSISSPAWAL
                     ADHAIIIEINLNEMGFNSFSCLYCLFDSIVAKIRKTRCKSISTRLHDLEELYSDFDLF
                     YE"
     gene            2306..3253
                     /locus_tag="ACZPD3_04560"
     CDS             2306..3253
                     /locus_tag="ACZPD3_04560"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR1415307.1"
                     /translation="MQEILDIFLEKSKQHHSNIIYAQIYKNDLLKEEFRLFPVKTRLN
                     IWSISKPFVAMAAGIAEREGLITLDEYIYPWFEKYFPSDPSENLYKIKIRDLLTMSSG
                     QKDPLFFCDGPERYIEKDWVRYFFQNDSFPYTPGTHFVYSNFCSYILSVLLEEKSGTG
                     LLNYLRYRFFEPVGIPNPDWTLCPQGHCMAANGLYLTIDELARFGHLLLHEGSLNGKQ
                     IVPKKFVIDACQKHIDSYNPLTEHPDYQSYGYGYFIYMGPMEDTLILSGNYGQYCVVD
                     KKHHMVSCVMSLDGNDHKKIRDDLVDSLAEYYGVKISHI"
     gene            complement(3345..4403)
                     /locus_tag="ACZPD3_04565"
     CDS             complement(3345..4403)
                     /locus_tag="ACZPD3_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR1415308.1"
                     /translation="MYVELKNINKTYGDYKASDNVSFGIEKGKLIGLLGPSGSGKTTI
                     LRMIAGLEQPDSGEIIIDGRVVNDVPASERGIGFVFQNYALFRYMTVYDNIAFGLKVQ
                     TADKKYIKQRVMELVELIGLKGLEKRYPSQLSGGQRQRVAFARALAPNPQLLLLDEPF
                     AAIDAKIRTELRSWLKDMIEKLGITSIFVTHDQDEAIEVADEIIITNRGRIEQKGTPL
                     EVYQNPDTAFTAGFFGQTSVIDDYQVFNHFEEVPGGEKAIVRPEFVKVTKKNEEQKYK
                     SSATEGIVERVAFRGSSLELKVRVGNTTLSARRSLDEEPVREGEVVDVFVQRIFVTKG
                     NEAVLLHNTAMVEDWLVI"
     gene            4602..4916
                     /gene="cas2"
                     /locus_tag="ACZPD3_04570"
     CDS             4602..4916
                     /gene="cas2"
                     /locus_tag="ACZPD3_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HIR1415309.1"
                     /translation="MEEIEFTTPDEKKSNEVFALIIYDIVDNKRRSQLVKYLQGYGFR
                     VQKSCFEVILNKSVFKKLKMEIAQYATDEDSIRIYQLHGIGQVTCYGNNFLVVPRDII
                     IV"
BASE COUNT         1467 a         1123 c          927 g         1453 t
ORIGIN      
        1 ggctcaaaca gcagagaaaa taatattttc cgttagaaat atgcattggt gatttttccc
       61 atgcttctgc atgttttttc ggaagctgga aataagcata aatctgcaga aatgccactg
      121 ctgatgccag aagcataata ttatttacaa catcactgat agaaaagtcc agtaacacag
      181 gtaatattcc cataagataa gtaaatgtca tcagtttcca gtaagcccct cttctgttct
      241 gagctgccca tgatttcgga agccatccat ctttacagga ctgtgcaacc ggaatacagt
      301 tattggaaat tgttgaatta atggatgaag acagtgcaag taccggacca ccaatcataa
      361 atactgtgaa aagtgccgga tttaaaatat tttttgcaac cagggtaagc ggctgtccgg
      421 ctacctgatc cagaggaagg acaccacttg ctacaattgc aacaccgcca tatataacca
      481 tcagtgtcgg aatacagtac agaagtgcct ttggaatatc tttatgcgct tcttttgcct
      541 gtttaccata attcataatg caggaatatc cgtttgttga atatacatac agcatgattg
      601 cggaaaagaa tccggatgga ccattggaga aaaagtctgg tgctgtaaac tcaaacaccg
      661 gatttttcag ctggaggcat cccagaacaa taaacagacc aagtgcaaca aacagcagga
      721 taaccagaat tttctgtacc ttcgccatta catccacacc acacagattg atggcaaaga
      781 acacactcag ggctgcaatt ccaaaccatt tcgcattaat ctgtggccac agggaatttg
      841 catacatacc aattgccaca ccataagtcg ccagattgat cgtctgtggc aaaaatccta
      901 ctgcatacat accggcaact cgtttgccgg ccatatctcc taccagagaa taaaatcctc
      961 ctccaagttt cagtgtagat gtgatccatg gaattggtgc aatcatcata aatccccaga
     1021 tacatgcagc tacatatgcc agccatgctg actgaccagt caaaagaatt gccggtccga
     1081 tcagtgtcag aacacccgat ccaatcgcct gtcctacacc gattgtataa agatcacttc
     1141 gtttcaggta acctgttttt tcttttattt cctttactgt tgacgacata aaagcccccc
     1201 ttttttattg actttaatat taatagtgta attaattctt tttatgacgt catatgaatt
     1261 atttaatgct atattaacat ctaggtttca agatttctat tgacaatatt atcaaaaata
     1321 cacactttag tttgtgcatt atttacattt tttttatatt aagaagaatt ttttactcca
     1381 ttatattgta atcaaacttt acacctgtta taatttatta tactaatcgt acagtacagg
     1441 aggttttcat gaaaatagaa cagttgatcc gtgaaaaagt atctttcctt tctgccggtc
     1501 agaagaaagt tgccgaatat atattacaac accttgattc attcagttat tccacccttg
     1561 cgaaactcag caaggaaatt tccgtaagcg aaaccactat catacgcctt gcctattccc
     1621 tgggatttga aagtttttct gaaatgcagc gtactgtaca gaatgatatt ctttccgttc
     1681 ctacggttat aaaagacgat tcactgctgg atagcggaaa tttttatcaa aacatcattg
     1741 ccaatgaact ccattccatg gaaagctggg catctcatct gaatcaggaa gatatggatc
     1801 agattgttac ccatctgagc aatgccaaaa aacgcctcat tgttggtgca cgctcttcct
     1861 acagtgccgc tctctggatg ggaactctgt tgaatcagct tcttggaaat accaaggtgg
     1921 ttcacgaatt ttacgattcc aatttcgaat atctcacaga agcatccgag gatacagttg
     1981 ttcttctgat ctcttttgcc cgctatacca aatggtctct gaaatatgca cagattgcca
     2041 aaaatcatgg tgcaaagata ctcgccatca cagacagcat ttcatcaccg gcatgggcgc
     2101 tggcagacca cgctatcatt atagagatca atctcaatga aatgggattt aactccttct
     2161 catgcctcta ttgtctgttt gacagtattg ttgcaaaaat ccgcaaaacc aggtgcaaat
     2221 ccatcagtac acgacttcat gacctagaag aactttattc agactttgat cttttttacg
     2281 aataataatt tcagggggga ttatcatgca ggaaatttta gatatttttc ttgagaaatc
     2341 aaagcaacat cattccaaca tcatctacgc acagatatat aaaaatgatc ttctaaaaga
     2401 agaattcaga ctttttccgg tcaaaacccg tttaaatata tggtctatca gcaagccttt
     2461 tgttgcaatg gccgcaggta tcgcagaacg tgaaggactg attactcttg atgaatacat
     2521 ttatccctgg tttgaaaaat atttcccttc cgacccttct gaaaatctgt acaagataaa
     2581 gatccgtgat cttcttacca tgtcatccgg tcagaaagat ccgctctttt tctgcgatgg
     2641 accagaacgg tacatagaaa aggactgggt gagatatttc tttcagaatg attcttttcc
     2701 ctatacaccc ggtactcatt ttgtgtacag caatttttgt tcctatattt tatctgttct
     2761 tctggaagag aaatccggaa cgggtctgct gaattatctg cgatatcgtt tctttgaacc
     2821 ggttggcatt ccgaatcctg actggaccct ctgccctcag ggacattgta tggcagctaa
     2881 cggactgtat ctgacgattg atgaattggc tcgttttgga catctgcttc tgcatgaggg
     2941 ttccctgaac ggaaaacaga tcgtcccaaa aaaattcgtg attgatgcct gtcagaaaca
     3001 tatcgattct tacaatccac tgacagagca tccggattac cagagctatg gttatggata
     3061 tttcatatat atgggaccta tggaagatac actgatcctg tccggcaatt acggccaata
     3121 ctgcgtcgtt gataaaaaac accatatggt atcctgcgtt atgtctttgg atggaaatga
     3181 ccacaaaaaa atccgtgatg atctggtaga ttcccttgca gaatattacg gtgtgaaaat
     3241 ttcacatata taaaaaaatg ccgttcccgc ggccacatca tatgatattc gcaatccgct
     3301 aacgctactt gctcataaaa aaatgccgct cccgcggcat ttttttatat cactaaccaa
     3361 tcctcaacca tagctgtatt atgcagcagt actgcctcat ttcccttcgt tacaaaaatc
     3421 ctctgcacaa acacatccac aacttcgcct tcccggacgg gttcttcgtc aaggcttctt
     3481 ctggcgctga gtgtggtgtt tcctacacgg actttcagtt ccaggctgct gccgcggaat
     3541 gcgactcgtt ctacgatgcc ctctgttgcg gagcttttat atttctgctc ctcgttcttc
     3601 tttgtcactt ttacaaactc cggacggaca atggcttttt cgcctcctgg aacttcctca
     3661 aagtgattga acacctggta atcatcgatc acagaagtct gcccgaaaaa tcccgccgta
     3721 aacgcagtat ccggattctg ataaacctcc agtggagttc ccttctgctc gatccgtcca
     3781 cggttggtaa taataatttc atccgccacc tcaatagcct catcctggtc atgggtaaca
     3841 aaaatactgg tgatccccag cttctcgatc atatccttca gccagcttcg aagctccgta
     3901 cggatttttg catcgatcgc cgcaaaaggc tcatccagaa ggagaagctg cggattcgga
     3961 gccagagccc gggcaaaagc aactcgctgt ctctgaccac cggacagctg actcggatat
     4021 cttttctcca gacccttcag cccgatcagc tctaccagtt ccataaccct ctgcttaata
     4081 tacttcttgt ccgccgtctg taccttcagg ccaaatgcaa tattatcata caccgtcata
     4141 taccggaaaa gagcataatt ctggaacaca aatccaatgc ctcgctcgct ggcaggcaca
     4201 tcattgacca ctcttccgtc gatgatgatc tcaccgctgt ccggctgctc cagaccggcg
     4261 atcatacgga gaatcgtagt ttttccgctt ccgctgggcc ccagaagacc gatcagcttc
     4321 cccttttcga taccaaagct tacgttatca gatgccttgt aatccccata tgttttattg
     4381 atatttttta actcaacgta catcgccttt ttccgagtta ggcgagaaaa tgatcgtcaa
     4441 tgccggtggt gacatctaat gagaacatcc caagataacg gaaagtggtg ttgaatcacc
     4501 actcctacca catctaatga gaacattcca agataaaaac cagaaaatgt caccctgttt
     4561 taaggagggt aatcctgttt taataaggtg agttggatga tatggaagaa atagaattta
     4621 caacacccga tgaaaaaaaa tcaaatgaag tatttgcgtt aataatatac gatattgtgg
     4681 ataataaacg gagaagtcaa ctggtcaaat atcttcaggg atatggtttt agagtacaga
     4741 aatcatgttt tgaagtaata ttgaataaat cagtgttcaa aaaattgaag atggagattg
     4801 cccaatatgc tacagatgag gacagtatcc gaatatatca gcttcatggt attggacaag
     4861 tgacatgtta tggtaataat tttctggttg tgccacgaga tatcattata gtataatgct
     4921 ttgtataaga tattatatcg taagaagtga ttattgaaaa aacaagctat
//
LOCUS       DBMDXG010000118         4967 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4156_length_4967_cov_1.855939, whole genome shotgun sequence.
ACCESSION   DBMDXG010000118 DBMDXG010000000
VERSION     DBMDXG010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4156_length_4967_cov_1.855939"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            213..443
                     /locus_tag="ACZPD3_04575"
     CDS             213..443
                     /locus_tag="ACZPD3_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415310.1"
                     /translation="MNKKKSKNKKQDKQSEKPVPRKKEEEQTEGTGRNSAQNPCYGLL
                     VCTGISYIYCLDFKNKNPGSYFNTSVSLADHY"
     gene            complement(409..1593)
                     /locus_tag="ACZPD3_04580"
     CDS             complement(409..1593)
                     /locus_tag="ACZPD3_04580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1415311.1"
                     /translation="MFETLGEKAEEKAMVQFLERFTDYPFLVKFKNSEYPIGEGEPTF
                     TVNFKETIPLAELLKSTSLALGEAYMRGDLDIEGNLYEALDHFLGQMSKFSTNESALK
                     KIMFSSTSKKNQEKEVTSHYDIGNDFYKLWLDETMSYSCGYFIHDDDSLYQAQVNKVD
                     YILKKLHLEEGMSLLDIGCGWGFLLIEAAKKYKVHGTGITLSHEQYTEFQKRIKDQGL
                     EDYLTVELMDYRDLPKHNYQFDRVVSVGMLEHVGRDNYQLFLDCVEKVLKPGGLFLLH
                     FISALKEHPGDPWIKKYIFPGGTVPSLREILNHMAEDNFHTLDIENLRLHYNKTLLHW
                     EKNFRENIEKEKTMFDESFLRMWELYLSACAATFHNGIIDLHQILMTKGINNDLPMTR
                     WY"
     gene            1982..2284
                     /gene="ureA"
                     /locus_tag="ACZPD3_04585"
     CDS             1982..2284
                     /gene="ureA"
                     /locus_tag="ACZPD3_04585"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HIR1415312.1"
                     /translation="MRLNPKEQEKLMLHMAGNLAKERKERGLKLNYVEALAYISSELL
                     ELARDGKTVVELMQLGTKILTKDDVMDGVADMIGEVQVEATFPDGTKLVTVHNPIQ"
     gene            2299..2658
                     /gene="ureB"
                     /locus_tag="ACZPD3_04590"
     CDS             2299..2658
                     /gene="ureB"
                     /locus_tag="ACZPD3_04590"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004055337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HIR1415313.1"
                     /translation="MKIGEIMAADREITLNEGKKTVTVTVANKGDRPVQVGSHFHFFE
                     VNKCLSFDREKAYGYHLDIPSGTSVRFEPGEEKEVQLTEMGGRQRVFGLNDLTRAQAT
                     DDTKAASMETAKLKGFL"
     gene            2671..4392
                     /gene="ureB"
                     /locus_tag="ACZPD3_04595"
     CDS             2671..4392
                     /gene="ureB"
                     /locus_tag="ACZPD3_04595"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HIR1415314.1"
                     /translation="MSTKISGSKYAAMYGPTTGDKVRLADTSLVIEVEKDYTTYGDEV
                     KFGGGKTIRDGMGQSVKTCSKDGDLDLVITNALIVDSTGIVKADIGIKDGKIAGIGKA
                     GNPDIMDGVTPGMTVGASTEALAGEGMIVTAGGIDTHIHFISPQQIDCALYSGVTTMI
                     GGGTGPADGTNATTCTPGPWNLKMMLKAAEEYPMNLGFLGKGNCSDEAPLIEQVKAGA
                     MGLKIHEDWGATPAVINHCLNVADEYDVQVAIHTDTLNEGGCVEDTLAAIGGRTIHTY
                     HTEGAGGGHAPDIIRAAAAPNVLPSSTNPTMPYTVNTLDEHLDMLMVCHHLDKRIPED
                     VAFADSRIRPETIAAEDVLHDMGIFSMMSSDSQAMGRVGEVITRTWQTASKMKDERGA
                     LPEDAGHDNDNFRVKRYISKYTINPAITHGISQYVGSVEEGKFADLVLWNPVFFGAKP
                     DIIIKGGMIIASKMGDANASIPTTQPVLYQPMFAAHGKTKNEACLTFVSQAAMDENVK
                     EKYGLEKTVVPVKGCRNISKKDMVFNDRTPELTVDPETYKVTVDGEEITSKPAEKLPL
                     TQLYSLF"
     gene            4565..>4967
                     /locus_tag="ACZPD3_04600"
     CDS             4565..>4967
                     /locus_tag="ACZPD3_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004055335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HIR1415315.1"
                     /translation="MLGVCLLFVGIVLINNGMCSLYNVDGKSTAIMNIFTGGLSLFIN
                     FVNLVQGNYYAAGTGLLFCFTYLFVALSKFLKASPIPFAWFSTFVAINAVIFGTIEGF
                     TGSTALGITPDLRWAAIWYLWAILWGTAFVED"
BASE COUNT         1567 a          980 c         1109 g         1311 t
ORIGIN      
        1 aacgtcggat tcgaaaataa gagaacgaaa gaatttctgg caaaatcctg taaatagaat
       61 aaatcttgat acttaaaact taaaatagtg agtaaatacc gggctttatt taccagtaac
      121 ttttacctgc atattacata gattttgatg taaatactct gcagatgata tattataaaa
      181 aatacaaata ttttcagaaa gtgcaggtta tcatgaataa aaagaaatca aagaataaaa
      241 aacaggataa gcagagtgag aagccggttc cgaggaaaaa ggaagaggag cagacagaag
      301 gaactggtag aaattcagct cagaaccctt gctatggatt actggtctgt actggaatat
      361 catatatata ctgcctggat tttaaaaata aaaatccagg cagttatttt aataccagcg
      421 tgtcattggc agatcattat tgatcccttt agtcatcaga atctggtgca gatcaataat
      481 tccgttatgg aaggttgcag cacatgctga caggtaaagt tcccacatac gcaggaagga
      541 ttcatcaaac atggtctttt ccttttctat attctcacgg aaattctttt cccagtggag
      601 cagagtttta ttgtaatgca gacgcagatt ttcaatgtcc agagtgtgga aattgtcctc
      661 tgccatatga ttcaggattt ctctcagact gggaacagtt ccgcctggaa agatatattt
      721 cttgatccat ggatcacccg gatgttcttt cagtgcactg ataaaatgaa gcaggaataa
      781 tccgcccggt ttcagaactt tctctacaca gtcaaggaag agctgataat tgtcacggcc
      841 aacatgttcc agcattccca cactgactac ccggtcaaac tgatagttat gtttcggcag
      901 atcacggtaa tccatcagtt ccactgtaag ataatcttcc aggccctgat ctttgatacg
      961 cttctggaat tctgtatatt gttcgtggct taaagtgatt cctgtaccat gtactttgta
     1021 ttttttggca gcctcaatca gcagaaaacc ccatccgcag ccgatatcaa gaagactcat
     1081 accttcttcc aggtgtaatt ttttcagaat ataatctact ttattaacct gtgcctgata
     1141 taaagagtca tcgtcatgaa tgaagtagcc acaggaataa ctcatggttt cgtccagcca
     1201 taatttataa aaatcatttc cgatatcata gtggctggtg acttcctttt cctgattttt
     1261 tttggatgta gaactgaaca tgatcttttt cagagcagat tcattggtag aaaatttgct
     1321 catctgtccc agaaaatgat ccagtgcctc gtaaagattt ccttcaatat ccagatctcc
     1381 ccgcatataa gcctcgccta atgccaggga agtgcttttt agcagctcgg ccagaggaat
     1441 tgtttcctta aaatttactg taaatgtggg ttctccctct ccgattggat attcggaatt
     1501 cttgaacttt accagaaatg gatagtccgt aaagcgttca aggaattgca ccattgcttt
     1561 ttcttcagcc ttttctccta atgtttcaaa cataatatac ctcctttaac acaaaactat
     1621 gatacacctt tgggggcgct tatcgtcaag gggagaatta aacaaagata tctgggaaat
     1681 tggtcagact tgtagcagaa taatggaaat agaagaagaa acaaaaagtt cacaaaaaac
     1741 atcttgacca tcaaagaaac acaagttata atcccctata taaaaatttt cgtgcaaaga
     1801 gcagacaatg gggtctgaga aagaatccca ttgtctgctc ttttttgcgt actacgaaga
     1861 tttttgttac tgttcacaca ccactatccc gcgaggtgtt ttggctacgt tacggagaac
     1921 ggagtgaaca gtaacagatt tttgcatatt ataatccgca caattcgaga ggaggaatat
     1981 catgaggctg aatcccaaag aacaggaaaa attaatgctt catatggcag gaaatcttgc
     2041 aaaagagaga aaggaaagag gtctgaaact gaattacgtg gaggcacttg cgtatatcag
     2101 ttctgaactc ctggagcttg caagagatgg taaaacagta gtagagctga tgcagcttgg
     2161 aaccaaaatc cttacaaagg atgacgtaat ggacggtgtt gctgatatga tcggtgaagt
     2221 tcaggtagag gcaacattcc cggatggaac caagcttgtg acagtacata atccaattca
     2281 gtaaggagga gaatgagcat gaagatcggt gaaattatgg ccgcagatcg tgagatcaca
     2341 cttaatgaag gcaagaaaac agtgacagta acagtggcaa acaaaggcga cagaccggtt
     2401 caggtcggtt ctcattttca cttctttgaa gtaaataaat gcctttcttt tgacagggaa
     2461 aaagcctatg gatatcatct ggacattcct tcaggaacat ctgtaagatt tgagccgggt
     2521 gaagaaaaag aggttcagct tacagaaatg ggaggcagac agagagtatt cggactgaat
     2581 gatctcaccc gcgcccaggc aactgacgat acaaaagcag catccatgga aactgcgaaa
     2641 ttaaaaggat ttctgtaagg agggacaaca atgagtacaa aaatttccgg aagcaaatat
     2701 gcggcaatgt atggcccgac tacaggagat aaggtacgtc tggcagatac aagccttgtg
     2761 atcgaggtag aaaaagatta tacaacctat ggagacgaag taaagttcgg cggcggcaag
     2821 acaatccgtg acggtatggg acagtctgta aaaacctgca gcaaagacgg agatctggat
     2881 ctggtaatta ccaatgcatt gattgtagac tctacaggaa tcgtaaaagc agatatcggt
     2941 atcaaagacg gcaaaatcgc aggaatcggt aaagccggaa atccggacat catggatggt
     3001 gtgacacccg gaatgacagt gggcgcatct acagaagcac ttgcaggaga gggaatgatc
     3061 gtaactgcag gtggaattga tacccatatc catttcatca gtccgcagca gatcgactgt
     3121 gcattataca gcggtgtaac tactatgatc ggtggtggta caggccctgc agacggtaca
     3181 aatgcgacta cctgtacacc tggaccatgg aacttaaaaa tgatgttaaa ggcagccgag
     3241 gaatacccga tgaacttagg tttccttgga aaaggtaact gctcagacga agctcctctc
     3301 attgagcagg tcaaggctgg agctatggga ctgaagatcc atgaggactg gggtgcaaca
     3361 cctgcagtta tcaatcactg tctgaatgtg gcagatgaat atgatgtaca ggttgccatc
     3421 catacagaca cattaaatga aggtggatgt gtagaagata cacttgcagc catcggcggc
     3481 agaaccatcc acacttatca tacagagggt gcaggcggtg gacatgcacc ggatatcatc
     3541 cgcgcagcag cagctccgaa cgtactgcca tcctccacaa acccaacaat gccatataca
     3601 gtaaacactc tggatgagca tctggatatg ctgatggtct gccatcatct tgacaagaga
     3661 atcccggaag atgttgcatt tgcagattcc cgaatccgtc ctgagacaat cgcagcagag
     3721 gatgttcttc atgatatggg tatcttcagt atgatgagct ctgactctca ggctatggga
     3781 cgtgtaggtg aggttatcac acgtacctgg cagacagcaa gcaagatgaa agatgagaga
     3841 ggtgcacttc cggaagatgc aggacatgac aatgacaact tccgtgtaaa acgttatatc
     3901 tccaaatata ccattaatcc ggcaatcaca cacggtattt cccagtatgt gggctctgtg
     3961 gaagaaggta aatttgcaga tctcgttctc tggaatcctg tattctttgg tgcaaaacca
     4021 gatatcatca tcaaaggcgg tatgatcatt gcatctaaga tgggcgatgc aaatgcatcc
     4081 attccgacta cacagccggt tctctatcag ccaatgtttg cagctcatgg aaaaactaag
     4141 aacgaagcct gcctgacctt tgtatcccag gctgccatgg atgaaaatgt aaaagagaaa
     4201 tacggtcttg agaaaactgt agtacctgta aagggatgca gaaatatcag caaaaaagat
     4261 atggtattta acgacagaac accggaactg actgtagatc cggagacata taaggttact
     4321 gttgacggcg aagagatcac ttccaaaccg gcagagaagc ttccgctgac acagctttac
     4381 agtctgttct aaacaacttt aaaccattac attctggtta tctgatcata aaaatgatat
     4441 tcgcaggact gttatttatg gagttttgtg agaactttta taagaactta ccaaacgata
     4501 taatgacagt cctgtcagat ttaacagaaa aatatactga agaagcagga gaggagagaa
     4561 tattatgtta ggagtttgtc ttttattcgt aggaatcgta ctgatcaaca acggtatgtg
     4621 ttctttatac aatgtagacg gaaaatcaac cgccattatg aacatcttta caggtggtct
     4681 ttctttattt attaactttg tcaatctggt gcagggcaat tattatgcag caggaacagg
     4741 actgctgttc tgttttacct atcttttcgt agccctgagt aaattcctga aagcaagccc
     4801 tatcccattt gcatggtttt ctacttttgt agccatcaat gcggtgatct tcggtacaat
     4861 cgagggattt acaggaagca ctgcactggg aatcacaccg gatctgcgtt gggcagcaat
     4921 ctggtatctg tgggcaattc tctggggaac tgctttcgta gaagata
//
LOCUS       DBMDXG010000119         4954 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4173_length_4954_cov_1.979394, whole genome shotgun sequence.
ACCESSION   DBMDXG010000119 DBMDXG010000000
VERSION     DBMDXG010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4173_length_4954_cov_1.979394"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="ACZPD3_04605"
     CDS             complement(<1..194)
                     /locus_tag="ACZPD3_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415316.1"
                     /translation="MKEKLLILFGIVLLLLVILVLRITYINATSGSKYKKQVLSQAQQ
                     KYESQVLPAKRGDIYDKNGN"
     gene            complement(260..838)
                     /locus_tag="ACZPD3_04610"
     CDS             complement(260..838)
                     /locus_tag="ACZPD3_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032153 - cell division site [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB/FtsL family cell division protein"
                     /protein_id="HIR1415317.1"
                     /translation="MARTTRSETARRSRTSARTNKGMYVDGNTVRRLAEVPERKRQPG
                     RQNPGRQQTNRNRRVQASPAQVSRQKHQLSKEAQKNRQKATAMNWGFVAFLAVICVAI
                     LFCSVKYLRYKSEITAKMSTVASLEEELADLKEDNDAYYSQVTSNVDLNKIKQIALGR
                     LGMKYPSDDQTVTYSTSGNSYVRQYQDIPDSK"
     gene            complement(854..1789)
                     /gene="rsmH"
                     /locus_tag="ACZPD3_04615"
     CDS             complement(854..1789)
                     /gene="rsmH"
                     /locus_tag="ACZPD3_04615"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR1415318.1"
                     /translation="MEFKHKSVLLEETIEGLDIKPDGIYVDGTLGGAGHAGEVCRKLS
                     AKGRFIGIDQDQDAIIAASERLAPYKQATIIRSNYCYMVQELAARGIYKVDGILLDLG
                     VSSYQLDNEERGFTYRVDAPLDMRMDQRQTQTAGDIVNGYEEKELYRIIRDYGEDKFA
                     KNIAKHIVAARQVKPITTTGELTEIIRESIPMKMQVKSGHPAKRTFQAIRIELNRELD
                     VLRDSLDGMIDILDDGGRLCIITFHSLEDRIVKTIFRKNENPCTCPSDFPVCVCGKKS
                     KGKVITRKPILPGETEMEENPRSKSAKLRIFERVL"
     gene            complement(1840..2271)
                     /gene="mraZ"
                     /locus_tag="ACZPD3_04620"
     CDS             complement(1840..2271)
                     /gene="mraZ"
                     /locus_tag="ACZPD3_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR1415319.1"
                     /translation="MFMGEYNHTIDAKGRLIIPSKFRELLGEEFVLTRGLDGCLYIYP
                     MDEWESFEMKLRSLPLTNKNARTFSRFFVAGATTCELDRQGRILVPQTLREFAGLEKD
                     VVLTGNLNRIEVWSKEKWNEICDYDDMDSIAESMQDMGITI"
     gene            complement(2565..3587)
                     /gene="lgt"
                     /locus_tag="ACZPD3_04625"
     CDS             complement(2565..3587)
                     /gene="lgt"
                     /locus_tag="ACZPD3_04625"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR1415320.1"
                     /translation="MSIRFPGLGLVFDYVPRSFQIFGMEFTIYGVLIAVGALLGMGLV
                     TLEAKRNGEDQNRYLDMMLISLLVSVAGSRLFYVAFSWGFYKENLNAILDFRNGGYAL
                     YGGLLFGFLAAAVFCRITKTSFWQSADIACPGILLGQAIGRWGNFFNRESFGEYTDLP
                     WAMQIPVSAVRSGEVSGAMRDNLLTINGISYIQVQPIFLYESLWCLLLFLLLMAMRRK
                     KKYEGELFMIYLAGYGLGRFFFEWFRTDKLYIPGTKVGISLVISAILFAVFMPVVIIR
                     RVMAQKRDTIRRQRRKRFLDESVKEKKMFPETSEMKPEPEIRPEMDEQVKPDVGNSPT
                     NSKEEQ"
     gene            complement(3608..4705)
                     /gene="ychF"
                     /locus_tag="ACZPD3_04630"
     CDS             complement(3608..4705)
                     /gene="ychF"
                     /locus_tag="ACZPD3_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR1415321.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKAGAESANYPFCTIDPNVGVVTV
                     PDERLKLLGDFYHSKKVTPAVIEFVDIAGLVKGASKGEGLGNQFLANIREVDAIVHVV
                     RCFEDSNVIHVDGSIDPIRDIETINLELIFSDLEILERRIAKVTKTARMDKEAAKELD
                     FLQKVKTHLEDGKMAITMEMENEDEEAWMSTYNLLTWKPVIYAANVAEGDLADDGESN
                     SHVQAVRKYAAEQNSEIFVICAEIEEEISELDDDEKKMFLEDLGLTESGLEKLVKASY
                     HLLGLMSFLTSGEDETRAWTIKIGTKAPQAAGKIHTDFERGFIKAEVVNYQDLLDCGS
                     YAGAREKGLVRMEGKEYVVQDGDVILFRFNV"
BASE COUNT         1289 a         1268 c          818 g         1579 t
ORIGIN      
        1 atatttccgt tcttgtcata aatatcgcct ctctttgccg gcagtacctg gctctcatat
       61 ttctgctgtg cctgacttaa cacctgtttt ttgtatttgc ttccactggt agcattaata
      121 taagtaattc tcagcactaa tattaccaga agcagtaaca caattccaaa caatatcagc
      181 agcttttctt tcatgttttg atttattttt ctctgtggtc ttcttcgact ctttcttccc
      241 gtattgctca attaatcacc tactttgaat ctggtatatc ctgatactgt cttacatagc
      301 tgttacccga tgtagaatag gttactgtct gatcatctga cggatatttc attcccagtc
      361 tccccaatgc aatctgcttg atcttattca gatccacatt tgatgtcacc tggctgtaat
      421 atgcatcatt gtcttctttt aaatctgcca gttcttcctc aagagatgca actgtactca
      481 tctttgcggt aatctctgat ttgtaacgaa gatatttcac agaacagaat aatatcgcca
      541 cacatataac tgccaaaaac gctacaaatc cccagttcat tgcagtcgcc ttctgcctgt
      601 tcttctgtgc ttctttactc aactgatgtt tctgccttga tacctgcgca ggactcgcct
      661 gcactcttcg gtttcgattg gtctgctgcc ttcccggatt ctgtcttccc ggctgcctct
      721 ttctctccgg aacttctgca agacgtctga ccgtatttcc gtcaacatac attcctttgt
      781 ttgttctggc actggttctg cttcttcttg ctgtttcact cctggtcgtt ctcgccattt
      841 catgcctctc taattataat actctctcaa aaatccgaag ctttgcactt ttggaacgcg
      901 gattctcttc catctctgtc tcccccggaa gaatcggctt tcttgtgatc acttttcctt
      961 tggatttctt cccgcatacg catacaggaa aatctgaagg acaggtacac ggattctcat
     1021 tttttctgaa aatcgtcttt acaattctgt cctccagaga atgaaaagtg ataatgcaaa
     1081 gtcttccgcc atcatcaagg atatcgatca ttccatccag agaatcccgc agcacatcga
     1141 gctctctgtt aagctcaata cggattgcct gaaaagttct cttggccgga tgtcctgact
     1201 tcacctgcat cttcatggga atggattctc tgatgatctc tgtcagttct ccggtggttg
     1261 tgatcggttt cacctgtctg gcggcaacaa tatgttttgc aatatttttt gcaaatttat
     1321 cttcaccgta atcacgaata atacggtata attctttttc ttcatagcca ttgacaatat
     1381 cgccggctgt ctgcgtctgg cgctggtcca tgcgcatatc gagcggtgca tctaccctgt
     1441 aggtaaaacc gcgttcttca ttgtcaagct gataggaaga tactccaaga tcaaggagaa
     1501 tcccgtcgac tttatagatg cctcttgctg ccagttcctg taccatgtaa caataattgc
     1561 tgcgaatgat cgtcgcctgt ttataggggg ctaacctctc actcgcagca attatcgcat
     1621 cctggtcttg atctatgcca ataaatctcc ccttggcaga aagtttcctg catacctcac
     1681 cggcatgccc tgcacctcct aaagtaccat ccacgtagat tccgtccggt ttgatatcca
     1741 aaccttcaat ggtctcttcc agtaatacag atttgtgttt aaattccatc tccgtctcct
     1801 gtctggattt actttgcaag atacttcaaa cgagacctgt cagattgtga ttcccatgtc
     1861 ctgcatgctc tctgcaatgc tgtccatgtc atcataatca cagatctcat tccatttttc
     1921 tttgctccaa acttcaattc tgttcagatt acctgtcaga accacatctt tctccagacc
     1981 ggcaaattcg cgcagtgtct gcggtacaag gattcgtccc tgcctgtcca gttcacatgt
     2041 agttgctcca gctacaaaga atcgtgaaaa agttctggca tttttatttg taagtggtaa
     2101 agatctgagc ttcatttcaa aggattccca ttcatccatc ggataaatgt aaagacagcc
     2161 atcaagccct cttgtcagaa caaactcttc ccctaagagc tccctgaatt tagaaggaat
     2221 gatcagacgc cccttcgcgt cgatcgtgtg gttatactct cccatgaaca tgttactcac
     2281 ctactctcgc aaaatctgaa tctccccttc agatttctca ggtggtaatg gactctgccg
     2341 ttattcccca ctcaccacca ctattcacca ctttctacca cttatgtgct tattctaaac
     2401 tatttaggct gaaaaaacaa gggggagaaa aagattttta aaatttagaa ctctgttaac
     2461 atttatgaaa caaccttatt tttacgagaa aaatgggctt ttatacacaa aaagtgggtt
     2521 gaatttccac tttcaaccca cttatctttc agatatatgt ttttttactg ttcttctttt
     2581 gaatttgtgg gagaatttcc cacatctggc tttacctgtt catccatctc cggccggatt
     2641 tccggttccg gtttcatttc agaagtctca ggaaacatct ttttttcttt cactgactca
     2701 tccagaaatc tttttctcct ctgccggcgt atagtatctc ttttctgtgc catcactctg
     2761 cgtatgatca ctacaggcat aaaaacagca aataatatag cggaaattac cagcgaaatc
     2821 cccacttttg tccccggaat ataaagctta tctgttctga accattcaaa gaaaaacctt
     2881 cccagaccat aacctgccag atagatcata aacaattcgc cttcatattt cttttttcgg
     2941 cgcatggcca tcaacagcag aaataacagc agacaccaca gactttcata aagaaaaatc
     3001 ggctgaacct gaatatatga aatcccattt atagtcagca gattatctct cattgctccc
     3061 gaaacttctc ctgaccgcac agctgaaacc ggtatctgca ttgcccatgg cagatctgta
     3121 tattctccga aagactctct gttaaaaaag ttaccccagc gccctatagc ctgtcccaga
     3181 agtattccgg gacacgcaat atcggcactc tgccagaatg acgttttggt aatcctgcaa
     3241 aataccgctg ccgcaagaaa tccaaagagc agaccaccat ataaagcgta accaccattt
     3301 cgaaagtcca gtatggcatt cagattttct ttataaaatc cccatgaaaa agccacataa
     3361 aataatctgg aacctgccac tgaaaccagc agggagataa gcatcatatc gagatacctg
     3421 ttctgatctt ccccatttcg tttcgcttca agtgtcacaa gtcccattcc aagaagagca
     3481 cccacggcaa tcagtactcc atatatggtg aattccattc caaatatctg aaatgagcgt
     3541 ggcacataat caaagaccag gccaagtccc ggaaaacgta tcgacatttc cataaatcac
     3601 ctgttcatca tacattgaat cggaataaga tcacatctcc atcctgtaca acgtattctt
     3661 taccttccat tcttacaaga cctttctctc tggcacctgc ataggaacca cagtccagaa
     3721 gatcctgata atttacaacc tcagctttaa taaatcctcg ctcaaaatct gtatggatct
     3781 tacctgctgc ctgcggagcc tttgttccga ttttaatcgt ccatgctctt gtctcatcct
     3841 cacctgaagt aaggaaactc atcagtccaa ggagatgata gcttgctttt acaagtttct
     3901 caagtcctga ttctgtcagt cccagatcct ccaggaacat tttcttctca tcatcatcca
     3961 gttcagagat ctcttcttcg atttctgcac agataacgaa aatctcactg ttctgctctg
     4021 ccgcatattt gcgcactgcc tgtacatggc tgttgctttc accgtcatca gccagatctc
     4081 cttctgctac gtttgctgca tagatcacag gcttccaggt aagcagatta taagtagaca
     4141 tccaggcttc ttcatcttca ttttccattt ccatagtaat tgccatcttg ccatcttcca
     4201 gatgtgtttt taccttctga aggaaatcaa gctccttggc agcctctttg tccatgcgtg
     4261 ctgtttttgt aacttttgca attcttctct caaggatctc aaggtctgag aagatcagct
     4321 ccagattaat agtttcaata tcacggatag gatcaatact tccatctaca tggataacat
     4381 tggaatcttc aaagcagcgg actacatgaa cgattgcatc tacctcacgg atatttgcaa
     4441 ggaactggtt tccaaggcct tctcctttgg atgctccctt tacaagtcct gcaatatcta
     4501 cgaattcaat tacagcaggt gtcactttct tagaatgata aaagtctccg agaagcttca
     4561 gtctctcatc cggcactgta acaacaccta cattagggtc aatggtacag aacggataat
     4621 ttgcagattc tgcgcctgcc tttgtcaatg aattaaataa tgtacttttt ccaacattgg
     4681 gtaatccgac gattcctaat ttcatttttt atatttcctc ctataaatcc tttgatttta
     4741 tcccagtcca atagctaaat ctttgattga ccaacatatc aaatgacaat caagtggata
     4801 ttcacaatcc aagctctttg ccttttaaat cttttcccat atttcagctt ctctcatcag
     4861 aagtcctaat acagtacttt gtgacaatta tataacgtcc tctatacaga aatatgcggt
     4921 tttaagccat tttggtggca aaagagccgc atat
//
LOCUS       DBMDXG010000120         4924 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4192_length_4924_cov_2.006428, whole genome shotgun sequence.
ACCESSION   DBMDXG010000120 DBMDXG010000000
VERSION     DBMDXG010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4192_length_4924_cov_2.006428"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="ACZPD3_04635"
     CDS             complement(<1..1310)
                     /locus_tag="ACZPD3_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015728.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HIR1415322.1"
                     /translation="MADKFINPYNFINFPAQKAKAYTDTDRHTGVIKYSITTESPLFI
                     PNSSSESAFSESTKVENHKSYDFFSYTELEAGKTYENEYHIPVIPGSEMRGVVRNVYE
                     TLTDSCMGVLNSEEYPVKRVPVRFKPALLCRNKEGMFELRDAFSTPVGDKAFNGKSPM
                     EYNNWRNGDLIVGKGYLLKWGMGGTGSKAKKRYHAFSEKSARAGEGRYKKNIVLSRDD
                     VERKLFPVISSYLSQPALQKNNKDAYIEYRKDLENFFKDKKKQYFPVNYSTVGKNLVY
                     LSPATVTKEAFHNSLGMLAGEFASCTENFCPACELFGHIGKNGDSSGSKIRFTDLYVT
                     DKRRPEEYYEFNKITLQALGGPKLGNTEFYLKQPDGATFWTYDYQVVDGKVIAKPGEL
                     RGRKFYWHSRNSRILKAEPSNFNKTVRPVTGKVTFSGDLYFEEIS"
     gene            complement(1312..1683)
                     /locus_tag="ACZPD3_04640"
     CDS             complement(1312..1683)
                     /locus_tag="ACZPD3_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415323.1"
                     /translation="MDLTSIPERGTLYALYRDKVKYEKYSRKELLEDKQLTEKLLELH
                     LFNDTREYRYIKTRSGEIETLISDETVEHEDIYTEKIVTLGNKKEKPDKDSGLVEVVN
                     YITYDENDLMRIENYRLKEVK"
     gene            complement(1689..3056)
                     /locus_tag="ACZPD3_04645"
     CDS             complement(1689..3056)
                     /locus_tag="ACZPD3_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015728.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HIR1415324.1"
                     /translation="MKTVIKRLVFYIQIQLESPLNVASGEDEWTDADILLDFDGNPFV
                     PGSSLAGAMRSYIGEKDNEWELFGFSKDDIEGKMSPLLISDLVFDQEPVIGVRDGVGL
                     NEKKVVKDGCKFDMEILETGLKGHFYLELVVREGDLEEEMKKQLAIVFQGIQQGEIRL
                     GRKKTRGFGAFKITRLSVRSYGKDNYLKYADAYKASTWEKASNELENWLHTDSDSRRM
                     IHIEVPLVMKGGISIRQYAAKKNEPDFVHITSNGVPVISGSSFAGAIRHRVEQILGEL
                     EAGGTKLPVKKKEILQIMFGYVEETAGNQATDAGDGARQSVNAGSGHASNVVISEARI
                     LDARPLTMVRTGISRFESAVHDGALYKEKTYVDGHFTLKIAVQKGKQPVDAKWMTGIL
                     LLAVNDLRNGYLAVGGQTAVGRGVFAPDTSEEYPKGVIFIDGKPRLENQFIAEAFNCL
                     CAHKR"
     gene            complement(3053..>4924)
                     /locus_tag="ACZPD3_04650"
     CDS             complement(3053..>4924)
                     /locus_tag="ACZPD3_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415325.1"
                     /translation="YEKVTAALKKCHHAELKSPQNVLNQYTYMRTQTAVNSETGTVQE
                     NSLRRIRVVKRGLVFTAECNWNRKVSFPELLGQAVSLVKHMGMSRTRGLGLVEMELIG
                     LDKAQKETLESDRWQHVLLDKNQLYDHNQIKYTVRLRSAMICKSTQGNQAATEDYIAG
                     SKILGLIAGALKPEGYSRLLESGEVIVTNGYITNGEERCVPGQISLQKVKDQRYDSNG
                     EMRIKDMLLTDPLEIRDKQMTPANIRYMDHTGTIEDVETEISYHHQRPSDKSVGRATG
                     LDGSSFYQLAAISPGQNFCGYIYAGREQTKQIIEAVEAMGEVRMGYGRSSEFGAVDFT
                     LDSVEKREEEHKIVRRAILTLGADVLLYNDQGMFTTDVRVLEKELCKMTGCRDLHLEK
                     PYIQFNAIGGYNVTWQRRKPAVYALAKGSTFLLHSENGFDMGLLNGKFAGERVTEGFG
                     ELLAKEPAESSDVVVKKVRAEKPAERMVEIMSQDTTGILDGLLQKEFEKRLEQAVREN
                     LKNENLFDQKQMDSMNAAIAKLRVLFGTEHSYENMKNEVREIEKESKNSLCTFLIERI
                     DPEEIGKQVEAEMTEAYGSSFKNKLTEERLFKKVYRAYLAELKYLVKTNQKKGDNKE"
BASE COUNT         1150 a         1278 c          862 g         1634 t
ORIGIN      
        1 gaaatctcct caaaataaag gtctccacta aaagtaactt ttcctgtcac cggtcttact
       61 gttttattaa aattggatgg ttctgcttta agaatcctgc tatttctgga atgccagtaa
      121 aattttcttc cacgcaattc acctggtttc gctataacct ttccatctac cacctgataa
      181 tcataggtcc agaaagttgc tccgtcaggt tgcttcaagt aaaattctgt atttccaagt
      241 ttaggtccgc ccagtgcctg aagagtgatt ttattaaatt cataatattc ctcaggcctt
      301 ctcttgtcag tcacgtataa atctgtaaat cgtatcttag aaccactgga atctccattt
      361 ttcccaatgt gaccaaacaa ttcgcatgcc gggcagaaat tttctgtgca tgaagcaaac
      421 tctccggcaa gcataccgag gctgttatga aatgcctcct ttgttactgt tgcaggagat
      481 aaataaacca gattttttcc gaccgtggaa taattaacag gaaaatactg tttcttttta
      541 tccttaaaga agttctccag atccttccgg tactctatat aagcatcttt gttgtttttt
      601 tgcagtgccg gctgagacaa ataggaacta atcaccggaa acagcttgcg ttctacgtca
      661 tctctggaaa gaacgatatt cttcttgtac ctgccttcac cagcacgagc acttttctcc
      721 gaaaacgcat ggtatctttt tttcgcctta cttccggtgc ctcccattcc ccatttcaga
      781 agatatccct ttccgactat cagatcacca ttcctccagt tgttatattc catcggactt
      841 ttgccgttaa atgccttgtc tcccactggc gttgaaaaag catctcttag ttcaaacatc
      901 ccctccttat ttctacaaag aagcgcaggc ttaaaccgga caggtacccg tttcaccgga
      961 tattcctcac tgttcagcac tcccatgcag gaatccgtta acgtctcata aacatttctc
     1021 accacacctc gcatctcact gcctggaatt accggaatat gatattcatt ctcataagtt
     1081 tttcctgcct ccagttccgt ataggaaaaa aagtcataag atttatgatt ctccaccttt
     1141 gtggattcac taaatgctga ttccgaactg gaatttggta taaaaagagg tgattccgtt
     1201 gttatgctgt attttatcac accggtgtgg cggtcggtat ctgtataagc tttcgccttt
     1261 tgtgccggaa aattgataaa attatatggg ttgataaatt tatctgccat cttatttcac
     1321 ctctttcaat ctgtaattct cgatccgcat aaggtcgttt tcatcgtaag taatataatt
     1381 tactacttct accagccctg aatctttgtc tggtttttct tttttatttc ccagagtgac
     1441 tattttttcc gtgtaaatgt cctcatgttc cacagtctca tcgctgatca gagtttctat
     1501 ttccccactt ctggtcttga tataacgata ctctctggta tcattaaata agtgaagctc
     1561 cagaagtttt tccgttagtt gcttgtcttc cagaagctcc ttccgactat atttctcata
     1621 cttcaccttg tcacggtata acgcatatag tgttccccgt tccgggatac ttgtcagatc
     1681 catgtttttc acctcttatg tgcgcagaga caattgaatg cttccgcgat gaattggttt
     1741 tccagtcttg gttttccgtc aatgaaaatc acacctttcg gatactcttc agaagtgtcc
     1801 ggggcaaaaa cccctcttcc tactgccgtt tgtcccccca cagccagata gccgttccgc
     1861 aggtcattca ctgccagaag aagaattcct gtcatccatt tggcatccac aggctgtttt
     1921 cctttttgca ctgcaatttt cagggtaaaa tgtccgtcca cataagtttt ttccttatac
     1981 aaagctccat cgtgtacagc agattcgaag cggctgattc cagtacggac catagtaagc
     2041 ggtctggcat ccagaattct tgcctcactg atcacaacat tggacgcgtg gccactgcct
     2101 gcatttacac tctgtctcgc tccatctcct gcgtctgtcg cctggtttcc agcggtttcc
     2161 tcaacgtatc caaacatgat ctgcagaatt tcttttttct tcacaggtaa ttttgtaccg
     2221 cctgcttcca gttctcccaa aatctgttct acccggtgac ggattgcacc tgcaaagctg
     2281 ctgcctgaga tcaccggcac tccgtttgaa gtgatatgga caaaatccgg ttcattcttt
     2341 tttgccgcat actgccggat actgatgccg cctttcatca caagagggac ttcaatatgg
     2401 atcattcttc tggaatccga atctgtgtgt agccagtttt ccagctcatt ggaagctttc
     2461 tcccaggtgg aagctttata ggcatctgca tatttcagat aattgtcttt gccataactt
     2521 ctgactgaca gtctggtgat cttaaatgct ccgaaacctc tggttttctt acgtcccaga
     2581 cgaatctctc cctgctggat tccctgaaac acaatagcaa gctgcttttt catctcttct
     2641 tccagatccc cctccctgac aacaagttcc agataaaaat gtcccttcag accggtttcc
     2701 aggatctcca tatcgaattt gcagccgtct tttacaactt tcttttcatt taaccctact
     2761 ccatctcgca caccgatcac aggctcctgg tcaaatacaa gatctgagat aagaagcggg
     2821 ctcatttttc cctctatatc gtctttggaa aagccaaaca gctcccattc attgtccttt
     2881 tccccgatat aagaacgcat ggcaccggca agggagcttc ctggcacaaa tggattgcca
     2941 tcgaaatcca ggagtatatc ggcatctgtc cattcatctt ctccagatgc tacattcagc
     3001 ggagactcca gctggatttg aatataaaaa accaaccgtt ttataactgt tttcattctt
     3061 tgttgtctcc tttcttctga tttgtcttta ctaagtattt cagttctgcc agatatgcac
     3121 ggtaaacctt cttaaaaagt ctttcttctg tcagtttatt tttaaagctg ctaccatagg
     3181 cttctgtcat ctctgcttcc acctgttttc cgatttcttc cggatctatc cgctcgatca
     3241 ggaaagtaca caaactgttt ttactttcct tttcaatctc tctgacctca tttttcatgt
     3301 tttcatagga atgttctgtt ccgaaaagga ctctcaactt cgctattgct gcattcatgc
     3361 tatccatttg tttctggtca aagaggtttt cgtttttcaa attttcccga acagcctgct
     3421 cgagcctctt ttcaaattct ttttgaagaa gtccgtccag aattccagtg gtatcctgtg
     3481 acattatttc taccattctt tctgccggtt tttctgcccg cacctttttt accacaacat
     3541 cagagctttc tgccggttct tttgccagaa gttctccaaa gccttccgtt acacgttctc
     3601 ctgcaaactt tccattcaaa agtcccatat caaatccgtt ttctgagtga agaaggaagg
     3661 tgcttccttt tgccagtgcg tatactgctg gtttccttcg ctgccaggtt acattgtatc
     3721 cgccgatggc attaaactgg atatatggtt tttccagatg aagatcccgg catcctgtca
     3781 ttttgcataa ttctttttct aaaacccgaa cgtcggtggt aaacattccc tggtcgttat
     3841 acagaagtac atctgccccc agcgtaagaa ttgcccgcct tactattttg tgttcttctt
     3901 ctcttttttc tacagaatcc agtgtaaaat ccaccgcccc aaactcactg gaacgtccat
     3961 accccatacg tacctcaccc atagcttcca cagcttctat gatctgcttt gtctgttctc
     4021 tgccggcata tatgtatccg cagaagttct gccccggcga aattgctgca agctggtaaa
     4081 aactggaacc gtccagtccg gtcgcacgtc ctacggattt gtctgacggt ctctggtgat
     4141 ggtaactgat ctctgtttcc acatcctcta tagtaccggt atgatccata taccggatat
     4201 ttgcaggtgt catctgctta tctctgattt ccagcggatc tgtcagaagc atatccttta
     4261 ttctcatctc tccgttactg tcgtaacgct gatcctttac cttttgcaaa gaaatctgtc
     4321 ctggcacaca tcgttcttct ccgtttgtga tataaccatt cgttacgatt acttcgccgc
     4381 tttccagaag gcgactatag ccttccggct tcaaggcacc ggcgatcagg ccaagaatct
     4441 tgctgcctgc aatataatct tctgtcgctg cctgattccc ctgtgtggat ttacagatca
     4501 tcgcacttct caggcgaaca gtatatttta tctggttatg atcataaagc tggtttttat
     4561 caaggagtac gtgctgccat ctgtcagatt ccaaagtctc tttctgcgct ttatccaacc
     4621 cgattagttc catttccacc agccccagcc ctcttgtcct agacattccc atatgtttta
     4681 caagggatac tgcctgtcca agaagttccg ggaaagatac ctttctgttc cagttacatt
     4741 ctgccgtaaa caccagacct cttttcacaa cccgaattct tctcagggaa ttttcctgga
     4801 cagtacctgt ttctgaattc actgctgtct gcgtccgcat ataagtatat tggttcagta
     4861 cattctgagg acttttcagc tctgcatggt ggcatttttt cagagcagct gtcactttct
     4921 cata
//
LOCUS       DBMDXG010000121         4907 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4203_length_4907_cov_2.000000, whole genome shotgun sequence.
ACCESSION   DBMDXG010000121 DBMDXG010000000
VERSION     DBMDXG010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4203_length_4907_cov_2.000000"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="ACZPD3_04655"
     CDS             complement(<1..1368)
                     /locus_tag="ACZPD3_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1415326.1"
                     /translation="MSIIFHENSKTFHLYNNEISYIMCVLPNGHLGNLYFGKRIHDRE
                     DFSYLLEMKQRPMTACVYEGNRKFSLEHLKLEYPVYGSSDYRYPAVEILQENGSRISD
                     FTYVSYTIAAGKPKLQGLPATYTEKDEEAQTLCVKLKDEVTGIVLELLYTIFVQGGII
                     TRSARFTNEGTSSVHLLNAMSLSLDLPDKDYVWMQFSGAWSRERHVKEHRLEQGIQSV
                     GSIRGNSSHEHNPFIVLRRLSATENAGEVMGFSLIYSGNHRMQAEVDTHDTTRITVGI
                     NPQNFDWKLDCGESFQTPEAVVVFSDKGLNGMSQTFHKLYQKRLARGYWRDRPRPILN
                     NNWEATYFDFTEDRLVEIAAKAKECGVELFVLDDGWFGARSNDYAGLGDWVANRERLP
                     QGIKGIADRIEEMGMKFGLWFEPEMVNKDSDLYRTHPDWILQTPQRHSCHGRNQYVLD
                     FSRKEV"
     gene            complement(1484..2479)
                     /gene="mgrA"
                     /locus_tag="ACZPD3_04660"
     CDS             complement(1484..2479)
                     /gene="mgrA"
                     /locus_tag="ACZPD3_04660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HIR1415327.1"
                     /translation="MYQANEKRYETMKYHRCGASGLMLPELSLGLWHNFGDTGVYENM
                     KQMCFTAFDNGITHFDLANNYGPQPGSAEENFGKILREEFSAYRDELIVSTKAGYDMW
                     PGPYGNWGSRKYLIASLDQSLKRLGLDYVDIFYHHRMDPETPLEETMEALAQIVRSGK
                     ALYVGISNYDGETMKRAADILEELHCPFIINQNSYNIFNRTIEKNGLKQAAAERRKGI
                     ISFSPLAQGMLTDRYLNGIPKDSRVNTDGRFLHADQFSEERLNSIRSLNAIATERGES
                     LAQMALKWILRDGIVTSVLIGASRPQQILENLKVLDSASFSEEELKKIDECSLAL"
     gene            complement(2604..>4907)
                     /gene="lacZ"
                     /locus_tag="ACZPD3_04665"
                     /gene_synonym="bga2A"
     CDS             complement(2604..>4907)
                     /gene="lacZ"
                     /locus_tag="ACZPD3_04665"
                     /gene_synonym="bga2A"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005990 - lactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase, LacZ type"
                     /protein_id="HIR1415328.1"
                     /translation="GSSALHSFVWKINNPKLWSAEDPQLYDLTIELYDEAGNIQEVIP
                     QKVGFRRFEMKNGIMTLNGKRIVFKGVNRHEFSSVSGRHVSEEELRKDLRIMKQNNIN
                     AIRTCHYPDTSLIYQLCDEYGIYMIDETNLESHGSWDVAEFTKDYTHVVPHNKPEWLD
                     MMLDRANSMYQRDKNHPAILIWSCGNESFGGKDIYEMSQLFRKNDSTRLVHYEGLFHD
                     RSYNDTSDMESQMYPSVEAIKEFLAKDDSKPFICCEYTHAMGNSCGAMHKYTDLTDTE
                     PKYQGGFIWDYIDQSIYKKDRYGKEFQAYGGDFGERPTDYNFSGNGIAYGGDREPSPK
                     MQEVKFNYQNITAEVTADTVKVINKNLFVNTNTFDCKVTLAKNGKVIRTEALETAVEP
                     LSTKGYKLPFGKAEAVGEYTVTVSFHLKEEKVWASAGHEIAFGQYVYQVKEDVPDGKS
                     DSAETAITAEKNVCVSDAFVKKPQIIRSTHNIGVRGEHFEVMFSVLNGGLVSYKYAGR
                     EMIEAIPKPNFWRAPTDNDCGNLMQMRYAQWKIASMYLSHKEYRKGAYGPSNLPQAEE
                     TDHSVKVTFTYLMPTTPASECQLTYEVFGDGKVKTTLTYDPVKELGDMPEFGVIFKFN
                     ADYDNVQWYGLGEAETYADRKKGAKLGIYQNKIVDNIARYMVPQECGAKEEVRWAKVT
                     DRKGRGMYFEMDGESMMFSALPYTPHEMENAMHPYELPQIHYTVVRVAKGQMGIGGDD
                     SWGAYTHPEYLLNADGKMEFSFSFKGI"
BASE COUNT         1234 a         1209 c          915 g         1549 t
ORIGIN      
        1 cacttctttt ctggagaagt ccagcacata ctgatttctg ccatgacagg aatgcctctg
       61 gggtgtctgc aggatccagt ccggatgtgt acggtaaagg tcggaatcct tattcaccat
      121 ctccggctca aaccacagac caaatttcat acccatctct tcaatcctgt ctgcgattcc
      181 cttgattccc tgcggaagac gctctctgtt tgccacccag tcaccaagtc ctgcataatc
      241 gttgcttcgt gcaccgaacc atccgtcatc cagcacaaat aattcaacac cacattcctt
      301 tgcctttgca gcaatttcca ccagacggtc ctctgtaaaa tcaaaataag tagcttccca
      361 gttattgtta aggattggcc tgggacggtc tctccagtaa cctcttgcca gtctcttctg
      421 atataattta tggaaagtct ggctcattcc attcagaccc ttatcagaaa atactactac
      481 tgcttccgga gtctgaaagc tctcaccaca gtccagcttc cagtcaaaat tctgcggatt
      541 gattcccaca gtgatgcggg ttgtatcatg agtatccacc tctgcctgca ttctgtgatt
      601 tccgctgtag atcaggctga atcccatcac ttctcctgca ttttctgttg ctgacagacg
      661 tcggagcaca ataaagggat tatgttcatg ggaagaattt cctctgatgc ttcccacaga
      721 ctggattccc tgctctaatc tgtgttcctt cacatgacgt tctctggacc atgcacctga
      781 aaactgcatc cacacataat ccttgtccgg cagatcaagg ctgaggctca ttgcatttaa
      841 cagatgtact gatgatgtgc cctcgtttgt aaatctggca cttctggtga tgattccccc
      901 ctgtacaaaa atagtataaa gcaattccag tacaattcct gttacttcat ctttcagttt
      961 tacacataag gtctgggctt cttcgtcttt ctctgtatag gtagccggaa gtccctgaag
     1021 ttttggtttt ccagctgcaa ttgtataact tacataagta aaatcagaaa tcctgctgcc
     1081 gttttcctga aggatctcca ctgccggata acggtaatca gaactgccat acaccggata
     1141 ttccagtttc agatgctcca gcgaaaattt gcggtttccc tcataaacac aggctgtcat
     1201 tggtcgctgc ttcatctcaa gaagatatga aaaatcttct ctgtcatgga ttcttttccc
     1261 aaaatagaga tttccaagat gtccatttgg aagcacacac ataatataac tgatctcatt
     1321 gttgtataaa tggaatgtct ttgaattttc atgaaaaata atactcataa ctcttccttt
     1381 ccgcatcttt gattattcac tgataatttc agaaaataat gcaattcaga ttatatgact
     1441 atttcatatc atttaagtaa atactctgtc aagcggatat ttattataat gccagtgaac
     1501 actcatcaat cttctttaat tcctcttcac tgaaagatgc actgtccagc accttcaagt
     1561 tctcaagaat ctgctgcgga cgggatgcac cgatgagaac gctggttacg attccgtcac
     1621 ggagaatcca tttcagtgcc atctgcgcaa gactttcccc tctttctgtt gcaattgcat
     1681 tcagactgcg gatactgtta agacgttcct cactgaactg gtctgcatgg agaaaacgtc
     1741 cgtctgtgtt gacacggctg tcttttggga ttccattcag gtatcggtct gtgagcattc
     1801 cctgcgccag aggactgaaa gaaataattc cctttctcct ctctgctgct gcctgtttca
     1861 gaccattttt ctcaatcgtt ctgttaaaaa tattatatga attctgatta atgataaacg
     1921 gacagtgcag ttcctccaga atatctgcgg cgcgtttcat tgtctcacca tcatagttgg
     1981 aaattccaac atacaatgcc tttccgcttc tgacgatctg tgccagagcc tccatagtct
     2041 cctccagagg agtctccgga tccattctgt gatgatagaa gatatctaca taatccaggc
     2101 caagacgttt caggctctgg tcaagactgg cgatcagata cttgcggctt ccccagtttc
     2161 cataaggtcc cggccacata tcatagcccg cctttgtgct gacgatcagc tcatctctgt
     2221 atgcagaaaa ttcctctctc aggatttttc caaaattttc ctccgcactt ccgggctgtg
     2281 gaccatagtt gtttgcaagg tcaaaatgag taatcccatt gtcaaatgcc gtaaagcaca
     2341 tctgcttcat attctcataa acaccggtat ccccgaaatt atgccataaa cccagagaca
     2401 actccggcaa catcagccca ctggcaccac agcggtggta tttcatagtt tcataacgtt
     2461 tttcatttgc ctgatacata agataatttc tccctttttc tgtaatcata catcctattc
     2521 catctatttt caacaaatgt tattcgtatt atttcaaaaa gccactttct gactgaaaat
     2581 ggctttttaa caaataaact atattaaatt cctttaaagc taaaggagaa ttccatcttt
     2641 ccgtctgcat tcagcagata ttccggatgg gtatatgctc cccagctgtc gtcaccgccg
     2701 attcccatct ggcctttggc tacgcgtact actgtgtaat gaatctgtgg gagttcatat
     2761 ggatgcatgg cattttccat ctcatgtgga gtgtatggga gtgcagagaa catcattgat
     2821 tctccatcca tttcaaagta cattcctctg cccttgcgat ctgtgacttt cgcccagcgt
     2881 acttcctctt ttgctccaca ttcctgcggt accatgtatc ttgctatgtt gtccacaatt
     2941 ttattctggt aaatgcccag ttttgcacct tttttgcgat ctgcataggt ctcagcttcg
     3001 cccagtccat accactgaac attgtcgtaa tctgcattga atttgaaaat caccccaaat
     3061 tccggcatat caccaagttc ttttacagga tcataagtta aagttgtctt cacttttcca
     3121 tcgccgaata cttcgtaggt gagctgacac tcactggctg gtgtggttgg catcagatag
     3181 gtaaatgtta cttttacaga gtggtctgtt tcttctgcct gtggtaaatt ggatgggcca
     3241 tatgcacctt ttctgtattc tttgtggctt aaatacatgc tggcaatttt ccactgtgcg
     3301 tagcgcatct gcataagatt cccacagtca ttatctgttg gtgcacgcca gaagtttggt
     3361 ttcgggattg cctcgatcat ctctctgccc gcatatttgt aagaaaccag accaccattg
     3421 aggacggaga acattacttc aaagtgctcg ccgcggacac caatgttgtg tgtgcttcgg
     3481 atgatctgtg gcttctttac aaacgcatct gatacacata catttttttc cgctgtaatc
     3541 gctgtttctg ctgaatctga ttttccatcc ggaacatcct ctttcacctg atatacatac
     3601 tgtccaaacg cgatctcatg gcctgcagat gcccacactt tttcctcttt cagatggaag
     3661 gaaacagtga ctgtatattc tccgactgct tcagcctttc caaaaggcag tttatatcct
     3721 ttcgtgctca atggctcaac tgctgtttcc agtgcttctg tacggatcac ttttccattc
     3781 tttgcaagtg ttaccttaca gtcaaaagta tttgtgttta caaacagatt cttgttaatc
     3841 acttttactg tatccgcagt tacttctgca gtaatattct gataattgaa tttcacttcc
     3901 tgcatcttcg gagatggttc tctgtcaccg ccatatgcga ttccgttacc actgaaattg
     3961 tagtctgtag gacgttctcc gaaatcgccg ccatatgcct ggaactcttt tccatagcga
     4021 tctttcttat aaatagactg gtcaatgtaa tcccagataa atccaccctg atatttgggc
     4081 tctgtatctg tcaggtcagt atatttgtgc atggcaccgc aggaatttcc catggcatgt
     4141 gtgtactcac agcagataaa cggtttactg tcatcctttg ccagaaactc tttgattgct
     4201 tctacagacg gatacatctg gctttccata tcactggtat cattataact gcggtcatgg
     4261 aaaagtccct cataatgtac cagacgggta gagtcattct tacggaaaag ctgagacatt
     4321 tcatagatat cttttccgcc aaaagattca tttccacagg accagatcag gattgccgga
     4381 tgattcttgt ctctctgata catggagttg gcacggtcaa gcatcatatc cagccattcc
     4441 ggtttgttgt gaggaacaac atgcgtataa tcctttgtaa attccgccac atcccaggaa
     4501 ccatgggatt ccaggtttgt ctcatcgatc atataaatac catattcatc acaaagctga
     4561 tagatcagtg atgtatccgg ataatggcag gtacggatgg cattgatatt attctgcttc
     4621 ataatgcgaa gatccttgcg gagttcttcc tctgatacat gtctgccgct gacagaactg
     4681 aattcatgac ggtttacgcc cttaaacaca atacgctttc cgttcagagt cataatgccg
     4741 ttcttcatct cgaaacggcg gaatccaacc ttctgaggaa tcacttcctg aatatttcct
     4801 gcttcgtcat aaagttcaat tgtaagatca tagagctgcg gatcttctgc actccataat
     4861 tttggattat tgattttcca tacaaaacta tggagtgcag aagatcc
//
LOCUS       DBMDXG010000122         4901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4208_length_4901_cov_1.909792, whole genome shotgun sequence.
ACCESSION   DBMDXG010000122 DBMDXG010000000
VERSION     DBMDXG010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_4901_cov_1.909792"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(165..1622)
                     /locus_tag="ACZPD3_04670"
     CDS             complement(165..1622)
                     /locus_tag="ACZPD3_04670"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR1415329.1"
                     /translation="MKRDFLKIRKRLIVGCLAAAIAVAQPVSSVFANPHYDRRDTVAE
                     EEFIYSARTSGTESSRKKVNSKAWKKINGVCYNGSGEIIPGAITRGMDVSEWQGNIDW
                     KQVKRSDIDFAFVRISYGLTHEDYTYDENMTNAELAGVPTGTYVYSTALSTTTALKEA
                     QLAISKMQGYKVSYPVVYDLEYAKASKLSAKTVSEMALTFCNEVRRAGYYPMVYCNTN
                     WYDNYIDWSLLSGVDVWIARYGDTIQAPDKERYNYTIWQSTDGNRESGLNSTSGLVAG
                     IPAGNDVDMDFGYVDYTKKITPRWKSLHSYVPAMKPDTGSNDGSQEQTGLHQENGKYY
                     YVNENGERVSDQWVTVNGKTYYISSDGYALMGMKKVDGKCYWFHTKSGYMFKNRRVTR
                     STGDIYYFGSDGVRCENGMYKVREKSGEHTYYFRKNGKAYKGWLTLNGKKYYFYKGSS
                     ALSGTRAENITLTSSNRIVSVFDGNGVCTRQYKKR"
     gene            complement(1822..4407)
                     /locus_tag="ACZPD3_04675"
     CDS             complement(1822..4407)
                     /locus_tag="ACZPD3_04675"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1415330.1"
                     /translation="MKRRLVAVTLSLIMTMATVSEAGAAAFTSPDTAPAVATQTDAEE
                     VNVTEETSETEADAFSDGDDGTAVPSQEQTDDAFTAGTDTAAALPDETPSTADATDAV
                     GAAVVKAEDWVSTENGFKLRKPAKNQDTANTDDQKAATAALPDEEMLSETDGFSGDQT
                     ESSETAVAETDTTAVTAEVTAMETPAATDTVSVTNEEFYTVADGIVKISTEYKGETHT
                     GYYLFDEEGILVTGQAEVKEKSSADEEPTSDAAQSAGEDATGEETEAQANQSYFTTAD
                     EAVVYTGCEGEAITPYTSTVGQQEKSVWKWTGTYFQYYDENGNLETIAQLEAKAKAAG
                     TYTGYFKINEDYYCLDSEGKPQTGEITLTVNGESNLYYFDPASSDIPGKMFHNGWLRS
                     DTTKGERWLYFKKGNIPADIGKYYKRGVVATAIPEKGTGDYLLDANGYVLKSVMKKAQ
                     NGAYYCTDSNGQIYRNKLVKYGNFRYYFGSNGKRATWTKRWAKAGDHYYYFGSTPGRV
                     VEKHGWQKLVSTSGKFLGWLYFDSKGNHYTDKWTSAGYYFKPSGKLASGLTEIDGKKY
                     IFESSTSAEHKGKVYKSTMVRYKKKWYIASSKGSLYKSGWRKYSGNYYYLKECVVQTN
                     QFMKKNGVNGYLDANGKYTRGWVIVSNAKNLVRYIDPSGNGFARNKSMRVNGILYYFD
                     SNGYRITDLTKRYRGPYSVQVDRVNGVMTVYADSARTIPVKTIRVSVGLAGTPTPTGN
                     FTLSRSLMWQPLMGPSWGQYGTHVDGAGQGGIFVHSVACGQANSYNLPAVEYNKLGSP
                     ASHGCIRTCVADAKWVYENCNGAPISIIDGKYKADDAMKGPLGKKALTPLRGAANFDP
                     TDPAV"
     gene            complement(4478..4741)
                     /locus_tag="ACZPD3_04680"
     CDS             complement(4478..4741)
                     /locus_tag="ACZPD3_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1415331.1"
                     /translation="MVSKKVTVKNPTGLHLRPAGILCNEAMKYQSQITFVYDGGMANA
                     KSVLSVLGACVKCGNEIELTCEGVDEQEALDHLVAAIDSGLGE"
BASE COUNT         1180 a         1229 c          864 g         1628 t
ORIGIN      
        1 ggaaaaaatc acgaaaggaa aaatcatggg aaacctgatc atcattgcag gtatcctgat
       61 ctatacgctt taattacggc aatttcataa aaagatcccg aagctgttgc agaatttcca
      121 tcagtgccac agctgcagga tctttttata tgtttcaata ttttttaccg ctttttatat
      181 tgtctggtac acactccatt tccatcaaac acagatacaa tcctgttgga actggtcagt
      241 gtaatgttct cggcacgcgt accggaaagt gcggaactgc ctttgtaaaa atagtatttt
      301 ttaccattca gtgtcagcca tcccttatac gcttttccgt ttttccggaa ataataggtg
      361 tgctccccac ttttttccct gactttgtac attccattct cgcaacgtac accgtcgctt
      421 ccaaaatagt agatatcgcc ggtagatctg gtcacgcggc ggtttttaaa catatatccg
      481 gattttgtat ggaaccagta acatttcccg tctactttct tcattcccat cagagcataa
      541 ccatcagaac tgatataata ggtctttcca tttacagtta cccactgatc tgacacacgt
      601 tcgccatttt cattgacata atagtatttt ccattttcct gatgaagtcc tgtctgttcc
      661 tggcttccat cattgctgcc tgtatcgggt ttcattgccg gaacatagga atgcagagat
      721 ttccatctcg gcgtaatttt ctttgtatag tccacatagc caaaatccat atcaacatca
      781 ttgcctgcag gaattcctgc taccagtccg ctggtactat tcagtccgct ttcccggtta
      841 ccgtctgtgg actgccatat tgtataattg taccgttcct tatcaggtgc ctgaatcgtg
      901 tccccatatc ttgcgatcca tacatccact cctgataata aggaccagtc tatgtaatta
      961 tcataccagt tggtattgca gtaaaccatt ggataatatc cggccctgcg cacttcattg
     1021 cagaaggtca gtgccatctc tgagactgtt tttgcagaaa gcttacttgc ttttgcatac
     1081 tccagatcat atacgaccgg ataggaaacc ttgtaaccct gcattttgct gatggcaagc
     1141 tgtgcttcct ttaatgcggt agttgtcgat aaggctgtac tgtacacata ggtgcctgtg
     1201 ggtactccgg caagttctgc atttgtcata ttctcatcat aggtataatc ttcgtgagtc
     1261 aggccatagg agatccggac aaacgcaaaa tctatatcag acctctttac ctgtttccag
     1321 tcaatattgc cctgccattc agagacatcc atgcctctgg ttatcgcacc cggaatgatt
     1381 tcaccacttc cgttatagca gacaccgttg atctttttcc atgctttgga gttcactttt
     1441 tttcttgatg actctgtacc ggaagttctt gcagaataaa taaattcctc ctcagccact
     1501 gtatcacgtc tgtcataatg cggatttgca aaaacagagc ttacaggctg cgctacagct
     1561 atcgcagctg ccagacaacc aacaataagc ctttttctga tttttaaaaa atctcttttc
     1621 atctttctac tcctgtcttc taataaacgt ccattttatt acaatactat taacatatta
     1681 tacacactat attacatttt cgcaagataa tccctgatat atacccaaaa acggctgcac
     1741 atttcttgct ctgtgcagcc gttttgcata tttcgtttct ataagcaatt atgtttattt
     1801 atcctggcag aaatatccgg atcagacagc cggatctgta ggatcaaaat tagcagctcc
     1861 tcttaacgga gtcagtgctt tctttccaag aggtcctttc attgcatcat ctgctttata
     1921 tttaccatca ataatactga tcggtgcgcc attacagttc tcataaaccc acttcgcatc
     1981 tgccacacag gttctgatac atccatgtga tgcgggagat ccaagcttat tgtactctac
     2041 tgccggaaga ttatatgagt ttgcctggcc gcaggctaca gaatgaacaa agattcctcc
     2101 ctgtccggca ccgtctacat gagttccata ctgtccccag gatggtccca taagaggctg
     2161 ccacatcagg cttctgctca gtgtaaagtt accggtcgga gttggtgttc ctgctaaacc
     2221 tacagatacc ctgatcgtct taacaggaat tgttctggct gaatcagcat aaacagtcat
     2281 aactccgttt actctgtcca cctgaacaga atacggtcct ctgtatcttt ttgtcagatc
     2341 agtgatgcgg tatccgttac tgtcaaagta atacagaatt ccattaactc tcatactctt
     2401 atttcttgca aatccgtttc ctgacggatc gatatatctc accaggtttt tagcattact
     2461 tacaattacc catcctcttg tatatttacc gtttgcatcc agataaccgt ttactccatt
     2521 ctttttcata aactgatttg tctgaaccac acattcttta agataatagt aattgccgct
     2581 gtattttctc catccgctct tatacaggct tcccttagag gatgcgatat accatttctt
     2641 tttatagcgc accattgtgc tcttatatac tttacctttg tgttctgcgg atgtagatga
     2701 ttcaaatata tatttctttc catcgatttc tgtcaaaccg cttgcaagct ttcctgatgg
     2761 tttaaaataa taacctgcgg aagtccactt atccgtataa tgatttccct tggaatcaaa
     2821 atacagccag ccaaggaatt tgccactggt gctgacaagt ttctgccagc catgtttctc
     2881 aacaacacga cccggagtac ttccaaagta atagtaatga tcgcctgctt ttgcccatct
     2941 ctttgtccag gttgcccgct ttccgtttga gccaaaataa tatctgaaat tgccgtattt
     3001 tacaagttta tttctataaa tctggccgtt actgtcggta cagtaatatg caccattctg
     3061 agccttcttc atcactgatt tcagaacata tccattggca tccagcaagt aatctccggt
     3121 tcctttctcc gggatagctg tggcaactac gccacgctta taatatttac cgatgtctgc
     3181 cggaatattt ccctttttaa aatacagcca gcgctctccc ttagtggtat ctgaacgaag
     3241 ccatccattg tggaacattt ttccgggaat gtcgctgctt gcaggatcaa aataatacag
     3301 gtttgattcg ccgttaactg tgagggtgat ctctcctgtc tgtggcttac cttcagaatc
     3361 aaggcagtaa taatcctcat tgatcttgaa atatccggta tatgtacctg ctgcttttgc
     3421 tttcgcttca agctgtgcaa ttgtctccag attaccattc tcatcgtagt actggaaata
     3481 tgttcctgtc catttccata cactcttctc ctgctggcct actgtagatg tatatggagt
     3541 tatcgcttct ccttcacatc cggtataaac aacagcctca tctgcagttg taaaatagga
     3601 ctgatttgcc tgggcttctg tttcttcccc tgtggcatct tctcctgctg actgagctgc
     3661 atctgatgtc ggttcttcat cagctgaaga tttttctttt acctcagcct gtccagttac
     3721 aaggattccc tcttcatcaa acagataata tcctgtatgg gtttcccctt tatactctgt
     3781 gctgatcttt acaattccgt ctgccactgt ataaaattct tcatttgtta cagacactgt
     3841 atccgttgct gctggtgttt ccattgctgt aacctctgct gtcacagctg ttgtatctgt
     3901 ttcagccact gctgtttcag atgattctgt ctgatctcct gagaatccgt cagtctcaga
     3961 cagcatctcc tcatcgggta gtgctgctgt tgctgctttc tgatcatctg tatttgctgt
     4021 atcctgattt tttgcaggtt tgcgcagttt aaagccattc tctgtgctta cccagtcctc
     4081 agctttcact acagcggctc caacggcatc cgtagcatct gctgttgacg gcgtctcgtc
     4141 cggcaatgca gcagccgtat ccgttcccgc tgtaaatgcg tcgtctgtct gttcctgtga
     4201 tggtactgct gtcccatcat ctccatctga aaaagcgtct gcttctgttt cagaagtttc
     4261 ttctgtaaca tttacttctt ctgcatctgt ctgtgtggca acagccggag ctgtatcagg
     4321 tgaagtaaat gcagcagcac ctgcttcact gacagttgcc attgtcataa taagactcaa
     4381 tgtaaccgca actaatcttc ttttcacaat aagttcctcc tcttttattc atgttacttt
     4441 atggttactg ttcactctgt ttgtgcacag cagctttcta ctcgccaagg ccgctatcaa
     4501 tagcagcaac aagatgatcc agggcttcct gttcatcaac accttcacag gtaagttcta
     4561 tctcatttcc gcattttacg catgcaccaa gtacactcag tacactcttt gcatttgcca
     4621 tgcccccatc gtagacaaat gttatctgtg actggtattt cattgcctca ttgcacaaaa
     4681 tccccgcagg tctcagatga agacctgtgg gatttttaac tgtcactttt ttactgacca
     4741 tttcactgtt cctcttatat tgttactacc ccttctgtaa gtggagaatt cttttattcc
     4801 ttgttttcct tactgtcatc aacagcagtc cctgatgaaa tcactacttc cgtagtcaca
     4861 tcttccactg tctcataacc atccggcaag actatctcaa c
//
LOCUS       DBMDXG010000123         4892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4218_length_4892_cov_1.677520, whole genome shotgun sequence.
ACCESSION   DBMDXG010000123 DBMDXG010000000
VERSION     DBMDXG010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4218_length_4892_cov_1.677520"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      203..247
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            360..1349
                     /locus_tag="ACZPD3_04685"
     CDS             360..1349
                     /locus_tag="ACZPD3_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR1415332.1"
                     /translation="MKKRKVIIDCDPGIDDSLAIMLALKSPEIEVIGITIVCGNSPVE
                     MGFGNAKKILKQMNRLDVPVYIGESTPLRRDYVNALDTHGEDGLGESFLPEVTGYQQQ
                     ISAVDFLADVLKKEKVSIIELAPMTNLARLIQKDKEAFSCIEEIVSMGGSFKSHGNCS
                     PVAEYNYWCDPDGASLVYETLHQNGQKIHMVGLDVTRKIVLTPDLLEYMCRLNKETGE
                     FVKKITKFYFDFHWEWEHIIGCVINDPLAVAYFINPELCKGFDSYVQIETEGISLGQS
                     VVDSMNFYRKTSNARVLTEVDVYGFFQMFLSRILDQEPEKLDILQDLIRGDLK"
     gene            1346..1903
                     /locus_tag="ACZPD3_04690"
     CDS             1346..1903
                     /locus_tag="ACZPD3_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1415333.1"
                     /translation="MKTEKISVQKICMIAFAICINFVGGQIALFLKLPIYLDSIGTVF
                     VAAVLGPFYGMLPNLLSGLLMGMTVDIYSLYYAPVGILLGFVTGLVYRKFQPKKWQIF
                     PAALVITLPSTIISSCITAFLFGGITSSGSTVLVQLLAKTPLGMVGSCFVVQFITDYI
                     DRVLCIAVAAVLITALRKSMKENFA"
     gene            1903..2553
                     /locus_tag="ACZPD3_04695"
     CDS             1903..2553
                     /locus_tag="ACZPD3_04695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR1415334.1"
                     /translation="MKVLITGTASGIGKGCAELFLKENHEVYGFDKNISSIQHPKYTH
                     FCLDIRDKSSYPEIGQVDILINNAGVQNGDDIDVNLKGTISVTEHYGIHPNIYSVIMI
                     GSASGHTGSEFPEYAASKGGVLAYTKNVAMRIAPYQATCNSLDFGGVMTELNRPVMED
                     EKLWDQIMELTPLKRWMSVEEAAEWIYFMAVKNRFCTGQNILIDGLEAGNCNFIWP"
     gene            2697..4055
                     /locus_tag="ACZPD3_04700"
     CDS             2697..4055
                     /locus_tag="ACZPD3_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR1415335.1"
                     /translation="MDIKEQIHGLTEEMLTNLGRLVAIDSQLGTPAEDKPFGEGPAKA
                     LEEGLKIAQELGFKTVNLDNYCGYAEMGEGDEIVGIAGHLDIVPVGGDWSYDPFKLTR
                     EGDYVYGRGTTDDKGPVLEALYAMKLLRDSGVKLNKRVRLIMGCNEETGSKCMEHYNE
                     VAEELSCGFTPDASFPCIHGEKGLLRMMAYSKNTKIISADGGFVFNAVCDSSTIVVPA
                     EEGLKERLEAVLAETKLQEYKVTEGNGQISIYAKGVPAHASTPTLGVNAIGVTFECLE
                     KAGFKDDFVEFYNTHIGTSCDGEGIGLKFADEYGELTLCNGMIKTENDVISCTIDIRV
                     PVTLKADEVRRMCESRLEDENGRIEILGIGDALFFPRESPLVNALYKAYTDVTGDTEN
                     KPMVIGGGTYAKSLKNIIAFGPEKPGIDYRIHSADEFILVSGMEEAVLVYLEAIKNLL
                     AI"
     gene            4095..4649
                     /locus_tag="ACZPD3_04705"
     CDS             4095..4649
                     /locus_tag="ACZPD3_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1415336.1"
                     /translation="MKAAVFFPGIGYHCDKPLLYYSRKLAQECGYEETIALSYTYDGG
                     NIRGNEEKMQQAFESLYEQAEKSLSAIDFDKYDEILFVAKSVGTIIASAYAEKHSIRC
                     RQILYTPLKYTYNFVHRDAIAFIGTSDPWSIVPEVQALSQKQQVPMYTYKNANHSLET
                     TDTLENLKILQDVMGKTKGFLERK"
BASE COUNT         1487 a          844 c         1196 g         1365 t
ORIGIN      
        1 aaagaaccgc gggacacgcg gggatagcct gttttagctt tgccctgaag ggcaattgag
       61 caggaagctc ccacttcaaa atctgtaaga tttaagtggc gggagcatgt cacgagattt
      121 ctgctcttag aatatgctgc aaatctcaaa tggtgtatgc gctaactgtt gtcatgcata
      181 aaaatatgtg ttaaaataac caccgaaact ggttcgaaac cctcccagta taaaaaacta
      241 ggcaaagtaa aaaatattgc ggtcgcttat gagttatcat aagcggtctg tttgctgatg
      301 cttttgttta ttgagggata aacaaaagta ttttttttgc tttgacagaa aggaattaaa
      361 tgaaaaaaag aaaagtaatt attgattgtg atccggggat tgatgacagt cttgcgatta
      421 tgcttgcact gaaatcaccg gagatcgaag tgatcggaat caccattgtg tgtggaaatt
      481 caccggtgga gatgggattt ggaaatgcaa agaaaatcct gaagcagatg aatcgtctgg
      541 atgttcctgt atacattggg gaaagcactc ccttaaggcg ggattacgtg aatgctctgg
      601 atactcatgg agaagacggg cttggcgaaa gctttctgcc ggaagtaacg ggttatcagc
      661 agcagatcag tgctgtggat tttcttgctg atgtattgaa aaaagagaaa gtatccataa
      721 tcgaactggc acctatgacc aatcttgcca gactgatcca gaaggataaa gaagcatttt
      781 cctgtattga ggaaattgtt tccatgggcg gcagctttaa aagtcatgga aactgttcac
      841 ctgttgcaga atacaattac tggtgtgacc cggacggggc ttccctggtg tatgagacac
      901 tccatcagaa tggtcagaaa atccatatgg tggggctaga tgtgacgaga aagattgtgc
      961 tcacgcctga tctgctggag tatatgtgcc gtctgaacaa agaaacaggg gaatttgtaa
     1021 agaagattac gaaattctat tttgatttcc actgggaatg ggagcatatc attggctgtg
     1081 tgatcaatga tcccctggca gtggcatatt ttatcaatcc ggaactctgt aaaggctttg
     1141 attcctatgt acagattgaa acagagggga tttctcttgg acagtctgtt gtggattcta
     1201 tgaatttcta ccggaaaact tcaaatgcaa gggtgctgac tgaggtagat gtgtatggat
     1261 tctttcagat gtttctttca aggatcctag atcaggaacc ggaaaaactg gatattttac
     1321 aagatttaat acgaggagat ttaaaatgaa aactgaaaaa ataagtgtac agaagatctg
     1381 tatgatcgct tttgctatct gcattaattt tgtgggagga cagattgctt tatttctgaa
     1441 acttcctatt tatctggaca gtattggaac tgtatttgtg gctgctgtcc tgggaccctt
     1501 ttatggaatg cttcctaatc tgctcagcgg cctgctgatg ggaatgactg tagatattta
     1561 ttctctgtat tatgctccgg tagggattct tcttggtttt gtgacaggtc ttgtgtacag
     1621 aaaattccag ccgaaaaagt ggcagatatt tccggctgct cttgtgatca cacttccatc
     1681 taccataatt agttcctgta ttacggcgtt tttatttgga ggaatcactt cttccggatc
     1741 caccgttctt gtacagcttc tggcaaaaac acctcttgga atggtaggaa gctgctttgt
     1801 ggttcagttt atcacagatt acattgacag ggttctgtgt attgcagtag cagcagttct
     1861 tattacagcg ctgcgtaaaa gtatgaagga gaattttgcc tgatgaaagt tctgatcaca
     1921 ggcacagcat ccgggatcgg gaaaggctgt gcagaattgt tcctgaaaga aaatcatgaa
     1981 gtctatggat ttgataaaaa tatatcctcg atccaacatc cgaaatatac tcatttctgt
     2041 ctggatatca gagacaaaag ctcttatccg gaaatcggac aggtagatat tctgatcaat
     2101 aatgcagggg ttcagaatgg cgatgatatt gatgtaaatc tgaaagggac gatttctgtt
     2161 acagaacatt acggcattca tcccaatatt tattctgtta ttatgattgg atcagcaagc
     2221 gggcatacag ggtctgaatt tccggagtat gcggcaagca aaggaggagt ccttgcctac
     2281 acgaaaaacg tggccatgag gatagctcct tatcaggcca catgcaacag tctggatttc
     2341 ggcggggtta tgactgaact gaaccggccg gtgatggagg atgaaaagct ctgggatcag
     2401 attatggaac ttacacctct gaaacggtgg atgtctgtgg aagaggctgc agagtggatt
     2461 tactttatgg cggtaaaaaa ccgcttctgt acaggtcaga acattctcat tgatggtctg
     2521 gaggccggaa actgcaattt tatctggcca tagagtgggt ctgaaaaact gtgtcctgaa
     2581 aaattatcta aaaaatcatt cctggaatct gctgttattt tgtgcctgaa tatgataaaa
     2641 tagtgataaa gatatttcaa agatagagaa agtatttaac tggaggcgca aaaattatgg
     2701 atattaaaga acagattcat ggattaacag aggagatgct taccaatctg ggaaggctgg
     2761 ttgccattga ttcacagctt ggcactcccg cagaggataa gccctttgga gaaggtcctg
     2821 ccaaagcact ggaagaaggt cttaagatag cacaggagct tggatttaaa acagtcaatc
     2881 tggacaatta ctgtggttat gcagagatgg gcgagggtga tgagattgtt ggtatcgccg
     2941 gacatctgga tattgtacct gtaggcggcg actggagtta tgatccgttt aaactgacac
     3001 gcgagggtga ttatgtttat ggtcgtggaa caacagatga caagggacct gttctggaag
     3061 ctctttatgc tatgaagctt ctccgcgatt caggtgtgaa gctgaacaaa agagtacgtc
     3121 tcattatggg atgtaacgag gaaacaggtt ccaaatgtat ggaacattat aatgaagtgg
     3181 cagaagaatt atcctgcggc tttaccccgg atgccagttt tccatgtatt catggcgaaa
     3241 aggggcttct gcggatgatg gcttattcca agaacacgaa aatcatctct gcagatggag
     3301 ggtttgtctt taacgcagta tgcgattcct ccacaatagt tgttcctgcc gaagaaggac
     3361 tgaaggagag actggaagct gtacttgcag agacaaagct tcaggaatat aaggtaacag
     3421 agggaaatgg acagatcagt atttatgcaa agggagttcc ggcacatgcc agcactccga
     3481 ctcttggggt caatgcaata ggtgttactt ttgaatgtct ggagaaagca ggatttaagg
     3541 acgattttgt ggaattctat aatacacata tcggcacatc ctgcgatggt gaaggcattg
     3601 gactgaaatt tgcagatgag tatggcgaac ttactctttg caacggtatg atcaaaacag
     3661 agaatgatgt gatctcctgt acaattgata tcagagtacc tgtaacattg aaagcagatg
     3721 aagtccgcag gatgtgtgag agcagacttg aggatgagaa tggtcgtata gagattctgg
     3781 gaattggtga tgccttattc ttcccaagag aatccccact ggtaaatgct ttatacaaag
     3841 cttacacaga tgtaacaggc gatacagaga acaagccgat ggtgatcggc ggtggaactt
     3901 atgcaaaatc tcttaagaat atcattgcat ttggacctga gaagccggga attgattatc
     3961 gtatccacag cgcagatgaa tttattctcg tatccggtat ggaagaggca gttctggttt
     4021 atctggaagc aattaagaat cttctggcaa tctgatgaaa aatgatggag cagctattaa
     4081 atggagagca gaacatgaag gcagcagtat ttttccctgg tattgggtat cattgtgaca
     4141 agccactttt atattattca cgaaagctgg cgcaggaatg tggctatgaa gaaacaattg
     4201 cattaagcta tacttatgat ggcggaaata tccggggaaa tgaagaaaaa atgcagcagg
     4261 cattcgaaag cctttatgaa caggcagaga aaagtctttc tgccattgac tttgacaaat
     4321 atgatgaaat attatttgtc gcaaaaagcg taggcacgat catagcatcc gcatatgcag
     4381 agaaacacag catcagatgt cggcagatac tatacacacc attgaaatac acatataatt
     4441 ttgttcatag agatgccatt gcctttatcg gaacttcaga tccatggagt attgttcctg
     4501 aagtacaggc tctttcacaa aaacagcagg tgccaatgta tacttataaa aatgcaaacc
     4561 attcactgga aacaacagat acactggaaa acctgaaaat tctccaggat gttatgggga
     4621 aaaccaaggg atttttggag cggaaataag aaaaagcaca taatcatatt gagtagatat
     4681 gggtgaagaa gtgtcaagaa actatttctt gacacttctt tttgctccat gctatactca
     4741 attagacaaa ctgttatatg tgaaagtggt ttccataatg tctttttagg tggtgatgtg
     4801 aatatgaact ttcaagatca gatacagcag atttttggaa caacagatat ttatgaactg
     4861 aagcagatat ccagagatgc tgacaattac ag
//
LOCUS       DBMDXG010000124         4870 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4238_length_4870_cov_1.808555, whole genome shotgun sequence.
ACCESSION   DBMDXG010000124 DBMDXG010000000
VERSION     DBMDXG010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_4870_cov_1.808555"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /gene="dnaK"
                     /locus_tag="ACZPD3_04710"
     CDS             <1..233
                     /gene="dnaK"
                     /locus_tag="ACZPD3_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022424.1"
                     /note="heat shock protein 70; assists in folding of
                     nascent polypeptide chains; refolding of misfolded
                     proteins; utilizes ATPase activity to help fold;
                     co-chaperones are DnaJ and GrpE; multiple copies in some
                     bacteria; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR1415337.1"
                     /translation="AKYANTDEIGDAQVAEIKAAQEKMMESAQKLFAKMYEQTQQAGG
                     QAGPDMNMGGGAAGSTDAGNNDDVVDADYKEV"
     gene            353..1540
                     /gene="dnaJ"
                     /locus_tag="ACZPD3_04715"
     CDS             353..1540
                     /gene="dnaJ"
                     /locus_tag="ACZPD3_04715"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR1415338.1"
                     /translation="MADKRDYYEVLGVDKSADDATLKKAYRKLAKKYHPDVNPGDKEA
                     EAKFKEATEAYTILSDPEKRKQYDQFGHAAFENGGGGAGGGFGGFDFNGADMGDIFGD
                     IFGDLFGGGSRSRRANNGPMKGANLRARVNITFEEAVFGCEKELEIVLKDECTTCHGT
                     GAKPGTSPVTCPKCHGEGQVVYTQQSMFGMVRNVQTCPDCHGSGKIIKDKCTSCRGTG
                     YTSSRKKIQVSVPAGIDNGQSIRIREKGEPGTNGGPRGDLLVEVNVACHPIFQRQDMN
                     IFSTAPLTYAQAALGGTVRINTVDGEVEYEVKPGTQTDTRIRLKGKGVPSLRNKNVRG
                     DHYVTFVVQVPTNLNEEAKEALRKFDEACGNRPKSKDSDDFEKPEKKKKSFMDKLKET
                     FED"
     gene            1672..2385
                     /locus_tag="ACZPD3_04720"
     CDS             1672..2385
                     /locus_tag="ACZPD3_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR1415339.1"
                     /translation="MTKTEKIAAFREKYLPVWVILPLISIFVLNCLIYWGSGVLTADR
                     YHFDFTMSLDRAVPLIPQFIWIYILAFPFWAVNYILAAQRGKDGFYRFVATDLTVHIT
                     CFIIFLVLPTTNVRPEIAGTTWSQQMLKFVYLMDGGNSPSNLFPSIHCYVSWLSWRGV
                     SKSENIPKWYQHFSLIFAILIIISTQVLKQHYLVDAIAGVALVELAWRFYSKGNRHEI
                     FRHFFEKISEACSRWVNKN"
     gene            2503..3456
                     /gene="prmA"
                     /locus_tag="ACZPD3_04725"
     CDS             2503..3456
                     /gene="prmA"
                     /locus_tag="ACZPD3_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR1415340.1"
                     /translation="MKWNRFTVKTKTEAEDIVISTLAEVGIEGVEIQDKQPLTEEDKA
                     QMFVDIMPEGPADDGIAYLNFYLEEDADKEAILKDVREALDDLKNFMDIGEATIEESQ
                     TEDKDWINNWKQYFHQFYVDDILIVPSWEEVKAEDKDKMILHIDPGTAFGTGMHETTQ
                     LVIRQLKKYVTPDTEMLDVGTGSGILGIVALKLGAKHVLGTDLDPCAVPAVAENKEAN
                     QIVDETFDMVIGNIIDDKAIQDQAGYEKYDIVTANILADVLIPLTPVIVNQMKKGAYY
                     ITSGILDVKEEVVVEAVKAAGLTVVEVTHQGEWVSVTARKD"
     gene            3461..4207
                     /locus_tag="ACZPD3_04730"
     CDS             3461..4207
                     /locus_tag="ACZPD3_04730"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR1415341.1"
                     /translation="MQRFFVEPYQIEEEAHRIHINGTDVNHIKNVLRMKCGEDVWISD
                     GGDKEYHCQIEELGEDEVLLHILYAQEPEYELPNKLYLFQGLPKADKMELIIQKAVEL
                     GAFSVIPVETKRCVVKLDAKKAAKKVVRWQQIAESAAKQSKRMLIPEIHEVMTYKQAL
                     EFAKQLDVKLIPYELAKGMKETREILSEIKPGQSVGIFIGPEGGFEEEEVAKALEAGA
                     HAITLGRRILRTETAGLAILSVLMFQLENE"
     gene            4229..>4870
                     /locus_tag="ACZPD3_04735"
     CDS             4229..>4870
                     /locus_tag="ACZPD3_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1415342.1"
                     /translation="MEAYFDNSATTRCFEEVKDIVVKTMMEDFGNPSAMHQKGVDAEG
                     YVKESARTLAQILKVTEKEILFTSGGTESNNLALIGGALANKRSGNHIITTAIEHAAV
                     SQPVAFLQEQGFEVTILPVDGHGVVKLDALEKALRPDTILVSTMMVNNETGAVMPVEK
                     IGAMIQEKCPKALYHVDAIQAFGKYRIYPKKWNIHLLSVSSHKIHGPKGVGFQI"
BASE COUNT         1549 a          865 c         1261 g         1195 t
ORIGIN      
        1 ttgctaaata tgcaaataca gatgagatcg gagatgctca ggtcgctgag atcaaagctg
       61 ctcaggagaa gatgatggaa agtgctcaga agcttttcgc taaaatgtat gagcagacac
      121 agcaggcagg cggccaggca ggaccggaca tgaatatggg cggcggtgca gcaggcagca
      181 cagatgcagg aaacaacgat gatgttgtag atgctgatta caaagaagta taatgtgaca
      241 tttctgtaaa agcagagatt tgcaacggaa atatcaataa taactgttca gtgggagagt
      301 ctctctcact gaatagttgt gttataaata aaacgggtat aggattaaaa tcatggctga
      361 taagagagat tattatgaag tcttaggcgt ggacaaaagc gccgatgacg caacacttaa
      421 aaaagcatat cgtaagctgg ccaagaagta tcacccggat gtaaatccag gagataaaga
      481 ggctgaggcg aaatttaaag aggcaacaga ggcttacacc atcttaagcg atccggagaa
      541 gagaaaacag tatgaccagt ttggccatgc ggcttttgaa aacggtggcg gaggtgccgg
      601 cggcggcttc ggaggttttg attttaacgg tgctgatatg ggtgatatct tcggcgatat
      661 tttcggcgat ttatttggcg gcggcagcag aagcagacgt gccaataatg gtccgatgaa
      721 gggagctaat ctccgcgcac gcgtaaatat tacttttgaa gaagctgtct ttggctgtga
      781 gaaggaactg gagatcgtac tgaaagacga gtgtaccacc tgtcatggaa caggtgcgaa
      841 gccgggtact tcaccggtaa cttgtccgaa atgtcatgga gaaggtcagg ttgtttatac
      901 acagcagtcc atgtttggaa tggtgcgtaa tgtacagact tgtccggatt gtcatggatc
      961 aggtaagatc atcaaggata aatgtacttc ctgccgcgga accggatata cgtcttcaag
     1021 aaagaagatt caggtatctg tacctgcagg tattgacaat ggtcagagca tccgcatccg
     1081 tgagaagggt gaaccgggta caaacggagg tccgagaggc gatctgcttg tagaagtaaa
     1141 tgttgcatgt catccgatct tccagagaca ggatatgaat atcttctcta ctgcgcctct
     1201 tacatacgcg caggcagcac tgggcggtac tgtacgtatc aacacagtgg atggagaggt
     1261 tgaatatgaa gtgaaacccg gaactcagac agacacaagg atccgtctga agggaaaagg
     1321 tgtaccgtct ctgagaaaca agaatgtacg tggcgatcat tatgtgactt tcgttgtgca
     1381 ggttcctaca aacctcaacg aagaagcaaa agaagcactg cgcaaattcg atgaggcgtg
     1441 tggtaaccgt ccgaaatcaa aagattctga tgattttgaa aaaccggaaa agaagaaaaa
     1501 gagtttcatg gataaattaa aagagacttt tgaggattaa ttataaaaga aaattaaaga
     1561 aaagaggttt accctctttt ctttttttgt atagtcgtgt atattgaaga gtaacggtaa
     1621 aaatcagatg aacagacagg aataaaagtt tcctgagaaa tggagagata tttgacaaaa
     1681 acagagaaga tcgcagcttt tcgggagaag tatctccctg tatgggtaat tttgccgctg
     1741 ataagcattt ttgtattaaa ctgtcttatt tactggggaa gcggagtact gacagcagac
     1801 agatatcatt ttgattttac catgtcactg gacagggcag tgccgttaat accacagttt
     1861 atctggattt atattctggc atttcctttt tgggcagtga attatattct ggctgcacag
     1921 agaggaaaag atggatttta tcggtttgtg gcaactgatc tgactgttca tattacatgt
     1981 tttattattt ttctggtttt gccaactacc aatgtcagac ctgaaattgc aggaactaca
     2041 tggtctcagc agatgcttaa gtttgtttat cttatggatg gtggaaacag tccgtctaac
     2101 ttatttccgt caatccattg ttatgtaagc tggctgagct ggcgcggagt cagtaaatca
     2161 gagaatattc caaaatggta tcagcatttt tcactgattt ttgcaatact gatcattatt
     2221 tctacccagg tgcttaaaca gcattatctg gtggatgcca ttgccggagt tgcactggta
     2281 gaattggcgt ggagatttta cagcaaagga aaccgccacg agatattcag acattttttt
     2341 gaaaaaataa gtgaagcatg cagtcggtgg gtaaataaga attgactgga tgtggtgaga
     2401 tgctacatta tgggaatagc tgcagagtaa cagagaatgg gccagacata ttttgggatg
     2461 ataatagtta attatataac ataaagaaat ggagacaatt acatgaagtg gaaccgtttt
     2521 acagtaaaaa cgaaaacaga agcagaagat atcgttatca gcacacttgc agaagtcggc
     2581 attgaaggcg tggaaattca ggacaaacag cctctgactg aggaagacaa ggctcagatg
     2641 tttgtagata ttatgccgga aggaccagca gatgacggca ttgcctatct gaatttttat
     2701 ctggaagaag atgcagacaa agaagcaatc ctgaaagatg ttcgtgaagc attagatgac
     2761 ctgaagaatt ttatggacat cggggaagct accattgaag aatctcagac agaggacaag
     2821 gactggatta ataactggaa acagtatttc caccagttct atgtagatga tatccttatc
     2881 gtaccgtcat gggaagaagt aaaagcagag gataaagaca agatgatcct ccacattgat
     2941 ccgggtacag ccttcggtac aggtatgcat gagactactc agcttgtgat tcgccagtta
     3001 aagaaatatg taacaccgga tacagagatg cttgacgtag gaaccggaag cggtatcctt
     3061 ggtatcgttg ccctgaaact tggtgcgaaa catgtactgg gaacagacct tgatccatgt
     3121 gcagttcctg cagttgcaga gaataaagaa gcaaaccaga tcgtggatga aacttttgat
     3181 atggtgatcg gaaacatcat tgacgataaa gcgattcagg atcaggcagg atatgaaaaa
     3241 tatgatatcg taacagcaaa tatccttgca gatgttctga tccctctgac accggtaatt
     3301 gtaaaccaga tgaaaaaagg cgcttactac attacttccg gtattcttga tgtgaaagaa
     3361 gaagttgtag tggaggcagt aaaagcagca ggcctgacgg tcgtagaagt gacacatcaa
     3421 ggcgagtggg tttccgtaac agcaagaaag gactgatcct atgcagcgtt ttttcgtgga
     3481 gccttatcag attgaggaag aggcacaccg tatccacatc aatgggacgg atgtaaatca
     3541 tattaaaaac gttctccgca tgaaatgtgg ggaggatgtg tggatcagtg acggaggaga
     3601 taaagaatac cactgtcaga ttgaagaact gggagaggat gaggttctgc tgcacatcct
     3661 ttatgcacag gagccggaat atgagctgcc caataagctt tatctttttc agggactgcc
     3721 gaaagcagac aagatggaac tgatcatcca gaaagcagtg gagctgggag ctttttccgt
     3781 aattccggta gagacaaagc gatgtgtggt gaagctggat gctaagaagg cggcaaagaa
     3841 ggtggttcgc tggcagcaga tcgcggagag tgcagcaaag cagtccaagc gaatgctgat
     3901 cccggagatt catgaagtaa tgacctacaa acaggcactg gaatttgcga aacagttaga
     3961 tgtaaaactg atcccgtacg aacttgccaa aggaatgaaa gaaaccagag aaatcctcag
     4021 cgagattaag cctggacagt ctgtcggtat ctttatcgga ccggagggtg gattcgagga
     4081 agaagaggta gcaaaggcac tggaagcagg tgcccatgcg attacacttg gaagacgaat
     4141 cctcagaact gagacagcag gacttgcgat tctttcagtt ctgatgttcc agttggaaaa
     4201 tgaataatag aaacaagaga gaacaaaaat ggaagcatat tttgataatt cagccacgac
     4261 ccgttgcttt gaagaagtaa aggacatcgt ggtaaaaaca atgatggaag attttggaaa
     4321 tccatctgcc atgcatcaga aaggtgtaga cgcagaaggg tatgtcaaag aatctgccag
     4381 aacgttggca cagatactga aagtaacaga aaaagaaatc ctgttcactt ccggcggaac
     4441 agagtcaaac aacctggctc tgatcggtgg tgcgcttgcc aacaaacgaa gcggtaatca
     4501 cattatcaca actgcaatag agcatgctgc agtcagccag ccagtagctt ttttgcagga
     4561 acagggattt gaagtgacca ttcttcctgt agacgggcat ggagtagtaa aacttgacgc
     4621 attggagaaa gcattaaggc cggataccat tctcgtatct acaatgatgg taaataatga
     4681 gaccggtgcg gttatgcctg tagagaaaat cggtgcaatg atccaggaga agtgtccgaa
     4741 ggcgctgtat catgtagatg cgatccaggc attcggtaaa tatcgtattt atccgaagaa
     4801 atggaatatc catcttctgt ctgtcagctc acataagatc catggaccaa aaggagttgg
     4861 attccagatc
//
LOCUS       DBMDXG010000125         4868 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4240_length_4868_cov_1.295784, whole genome shotgun sequence.
ACCESSION   DBMDXG010000125 DBMDXG010000000
VERSION     DBMDXG010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4240_length_4868_cov_1.295784"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            2..841
                     /locus_tag="ACZPD3_04740"
     CDS             2..841
                     /locus_tag="ACZPD3_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1415343.1"
                     /translation="MKSYLTSLVGYLFIFFILVLTGIYFSAYQLSATYPKFEYTLSAI
                     TFAFLIGVPIFTMRVLAEERKQKTDQLLLTAPVSVSGIVIGKYLALVTVFAVPMAVMC
                     TYPLIMSKFGTVEFASAYTAVLGFFLLGCANIAIGVFMSALTESQVIAAVLTFVFLFA
                     FYMMNGISSFFSQTSMSTCVTFGLLILAAAIIIYTMIKNILISAVIGVIGEVALIIIY
                     VVKSSIFEGGIQKVLDVFNLSGHFDNFTSNIFDIKGIVYFLSVIAVCLFLTTQSILKR
                     RWN"
     gene            859..2343
                     /locus_tag="ACZPD3_04745"
     CDS             859..2343
                     /locus_tag="ACZPD3_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR1415344.1"
                     /translation="MKLKEKMKNNKHKKFDKKKLIGTISKKHIKNGSYTMVMSVVFIA
                     VVVVLNMIVNAIPSKYSEIDISSQKLYSIGDDTKAMLKDLDKDVTIYQIAQSGSEDEN
                     ITNLLKKYEDESKHIKVEQKDPVVNPKFVTEYTSDDLSANSLIVVCGDRNKVIDYNNI
                     YESTMDYQTYSSQTTGFDGEGQITSAIGYVTSEDLPILYTLEGHGEKEMDSTIKEDIE
                     KANMDIQSLNLLTEGSVPDDADCLFIDSPSTDISEDEKTAIIDYLENGGKAMIFSDYT
                     TEDLPNFDAVLENYGVQREAGIVFEGDNQHYAMQMPYYLVPTINSTDASSETVSGGYY
                     VLVPYAQGIKKMDDVRDTVTINSILTTSDQAYSKTDLNSDTLEKEDGDEAGPFDLGVS
                     ITETLDDDKETQIVYYSTSNLMESQVNQMVSGGNEKLIIESLKWMTDTEDSATVSIPS
                     KSLSVSYLTLTDYDAAFWKICTIGLIPGFFLVVGFAVWMKRRKA"
     gene            2344..3987
                     /locus_tag="ACZPD3_04750"
     CDS             2344..3987
                     /locus_tag="ACZPD3_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR1415345.1"
                     /translation="MKNKTVKMVLAVVVLGVCCGAYAGVKTYVAHQEQKESEEDSEES
                     TTVFTASTDNIKSLDFMVDDTKTTFEKDDDSWVKKDETDFPVNQTTLDSAASAIASVD
                     SDRVLEDVDDLSEYGLDSPSNTIKIVTKSDEEDGDDITTTLYVGDENSSTSQYYVRKD
                     DDEKTVYLIDSSCVEPFTKSLNDYAQMDDFPAISNTDTITKISVDGDNSYELSKDEDT
                     STWSVKGSEDEEKADSATVSSLVSSFGSMAYSSLADYKCDDKSKYGLDKPYATITVDY
                     QEEVADDDTQDNTTAPSEAADEDASSAEENSDDAKQDTESDDEDSSEEETKMADKQLT
                     ILVGNKTDDSNRYVMVNDSNEVYTMSTDSLSALTDKSEEDFWDMTVSYVSLNSLGSLK
                     VNYQGSDYKVNVSRETSTDDDGNDTETVTYKLNGSDLDETTFTTFYNKLINMTAQKRL
                     TDKYEPDGDAELTAIFTEEDGDTQEVAYYSYDTNYYAAVVDNKVYLVNKMNVKELFTA
                     FESVAGTEEDNSGETDSEEATTDDTKSDLTDTSSESIEE"
     gene            4068..4154
                     /locus_tag="ACZPD3_04755"
     CDS             4068..4154
                     /locus_tag="ACZPD3_04755"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415346.1"
                     /translation="MKNYSHNLSAPLCGIFCPNSVASTSFRK"
     gene            complement(4417..>4868)
                     /locus_tag="ACZPD3_04760"
     CDS             complement(4417..>4868)
                     /locus_tag="ACZPD3_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1415347.1"
                     /translation="LKQMPNRKDRLLKAALEIPIFGTLVYHMIVSRDNINNLFIEKMY
                     YNPFHVDNQMSDAYYEAAHKGGYYTRFLYSSLAAKYMNVNICHALKALDNSIYIVEGE
                     TEPNGKAVTDDYCASNPAIEVSVLKETKHLPHVEAPEAFLEQVKIFF"
BASE COUNT         1609 a          868 c         1035 g         1356 t
ORIGIN      
        1 gctgaaaagt tacctgacat ccctggtagg atatttattt atctttttca ttcttgtact
       61 gacaggtatt tatttttcag cataccagct ttcagcaaca tatccgaaat ttgaatacac
      121 cttaagtgca attacctttg cattcctgat cggagtaccg atttttacta tgcgtgtact
      181 tgcagaggaa agaaaacaga aaacagatca gctgcttctg acagcaccgg tttctgtgag
      241 cggtattgta ataggtaaat atcttgcact ggtaactgta tttgctgttc cgatggcagt
      301 tatgtgtact tatccgctga ttatgtccaa gtttggcaca gtagagtttg catctgcata
      361 tacagcagtc ctgggattct ttctcctggg atgtgcaaat attgcaatcg gtgtatttat
      421 gtcagcatta actgagagcc aggtaattgc agcggtactc acatttgttt tcttatttgc
      481 attttacatg atgaatggta tttcttcatt cttctctcag acatctatgt cgacatgcgt
      541 gacatttggg ttattgatcc tggcagcagc catcattatc tataccatga tcaagaatat
      601 tctgatctca gcagtgatcg gtgtgatcgg agaagtggca ctgattatta tttatgtagt
      661 aaaatccagt atttttgagg gcggtattca gaaagtcctg gatgtgttta atctgagcgg
      721 acactttgat aattttacaa gtaacatctt tgatataaaa ggaattgtat attttctttc
      781 tgtgatcgca gtatgcctgt tccttacaac acagtctatt ttgaagagac gctggaatta
      841 gtggggaggt gaaagaaaat gaaactgaaa gaaaagatga aaaataataa gcataaaaaa
      901 tttgacaaaa agaaactgat tggcaccatc agtaagaagc atatcaaaaa cggctcttac
      961 acaatggtta tgtcagtggt atttattgca gtagttgtag tgctcaacat gatcgtaaat
     1021 gcgattccgt ccaaatattc agaaattgat atcagcagcc agaaactcta cagtattggt
     1081 gatgatacaa aagcaatgct gaaagatctg gacaaggacg taacaattta tcagattgca
     1141 cagagcggtt ctgaagatga gaatattacc aatctgctta agaaatatga agatgaatcc
     1201 aagcatatta aagtagagca gaaggatcct gtagtaaatc ctaaatttgt aacagaatat
     1261 acaagtgatg atctttctgc aaacagtctg atcgttgtat gcggcgacag aaataaagtc
     1321 attgattaca acaatatcta tgagagtaca atggactatc agacctacag ctctcagacc
     1381 actggtttcg acggtgaagg tcagatcaca agtgcgatcg gatatgtaac atccgaagat
     1441 ctcccgatcc tttatacact cgagggacat ggtgagaagg agatggattc tactatcaaa
     1501 gaagacatcg agaaggcaaa catggatatt cagtctctga atcttcttac agagggaagt
     1561 gttccggatg atgcagactg cctgttcatt gattctccat ccacagatat ttcagaagat
     1621 gaaaagactg ctattattga ttatcttgaa aatggcggaa aggcgatgat tttctctgat
     1681 tatacaacag aagacctgcc aaactttgat gcagttctgg aaaactatgg tgttcagaga
     1741 gaagctggaa tcgtatttga aggtgataat cagcattatg caatgcagat gccatattat
     1801 ctggttccga ctataaacag cacagatgca agctctgaga ctgtttccgg aggatattat
     1861 gtacttgtac catatgcaca gggaatcaaa aaaatggatg atgtaagaga taccgtaacg
     1921 atcaacagta tcctcaccac atcagaccag gcatattcca agacagatct gaacagtgat
     1981 actctggaga aagaagacgg agacgaagct ggcccatttg atctgggagt aagcatcaca
     2041 gagactctgg atgatgacaa agaaacgcag atcgtatact attcaacttc caatctgatg
     2101 gaatcacagg taaatcagat ggtatctggc ggaaatgaga agctgattat cgaatccctg
     2161 aagtggatga cagacacaga agacagtgca accgtatcta tcccatccaa gagcctgtct
     2221 gtatcttatc tgacacttac agactatgat gctgcattct ggaagatctg caccatcggt
     2281 ctgattcctg gattcttcct tgttgtaggg tttgcagtct ggatgaagag gagaaaagct
     2341 taaatgaaga ataaaactgt aaaaatggtt cttgcagttg tagttcttgg tgtctgctgc
     2401 ggggcttacg caggtgtaaa aacctacgta gcccatcagg agcagaaaga atccgaagaa
     2461 gattctgaag aaagcacaac tgtatttact gcatcaacag ataacattaa atctctggac
     2521 tttatggttg atgatacaaa gacaacattt gaaaaagatg atgacagctg ggtaaagaaa
     2581 gacgaaacag attttccggt aaatcagact actctggaca gtgcagcatc tgctattgca
     2641 tctgtcgatt cagacagagt tctggaggac gtagatgatc tttcagaata tggtctggac
     2701 tctccgtcaa acacgattaa gattgtaaca aaatctgatg aggaagatgg cgatgacata
     2761 acaactacac tgtatgtggg agatgaaaat tcctccacca gtcagtatta tgtaagaaaa
     2821 gatgatgatg agaagacagt atacctgata gattcttcct gcgtagagcc gtttaccaaa
     2881 tcattgaatg attatgcgca gatggatgat ttccctgcaa tttccaatac agataccatt
     2941 acaaaaattt ctgtagacgg cgataattcc tatgaattat caaaagacga agatacaagt
     3001 acctggtcag taaaaggcag cgaggatgaa gaaaaagcag actcagctac cgtaagttct
     3061 ctggtatcct cattcggaag catggcatat agttcgcttg cagattataa atgtgacgac
     3121 aagagcaaat atggtctgga taaaccatat gcaacaatca cagtagatta tcaggaagaa
     3181 gttgctgatg atgacacaca ggataatacg acagctccca gtgaggcagc agatgaagat
     3241 gcttcatctg cggaagaaaa cagtgacgat gctaaacagg atacagaatc agacgatgag
     3301 gattcttcgg aagaagaaac taagatggca gataagcagc ttacaattct tgttggaaat
     3361 aaaacggatg acagcaacag atatgttatg gtaaatgaca gcaatgaagt ctatacgatg
     3421 tcaacggatt ctctgtccgc actcactgat aaatcagaag aagacttctg ggatatgaca
     3481 gtaagttatg tatctttaaa cagcctcgga agcctcaaag taaattatca gggcagtgat
     3541 tataaagtaa atgtttcacg tgaaacatcc acagatgatg atggaaacga tacagaaaca
     3601 gttacctata aactgaatgg ttctgatctg gatgaaacaa catttacaac attctacaat
     3661 aaactgatca acatgacggc tcagaaacgt ctgacagata aatatgaacc tgacggtgat
     3721 gcagaactga cagctatatt tacagaagaa gacggtgata cacaggaagt tgcatactat
     3781 agttacgata caaactatta tgcagcagtg gtggataata aagtatatct cgtaaataag
     3841 atgaatgtaa aagaactgtt tacagcattt gaatcagttg ccggaacaga agaagataat
     3901 tcaggtgaga cggattcaga agaagcaacg acagatgata ctaaatcaga tttaacagat
     3961 acgtcaagtg agagcataga agagtaaaag aatctataaa aaagtggagc aggcatatag
     4021 aattttttcc atatgtctgc tccgcttttt gttgtattag agcgtgtttg aaaaattatt
     4081 cccacaatct atccgcccca ctttgcggta tattttgtcc gaattcggtt gctagtacat
     4141 cgtttcgtaa ataactatac caatcgcgta ggatgcggat tcgagtgtgc aggtctaaaa
     4201 acgcaggcgt agcgggctac gctgaggctt ttagacctgt gcactcggat ttgcagacaa
     4261 gtgagtggta tagttatttc gaaaacgatg tactagagtc aacgcaacgt cgtctttttc
     4321 cgacttgaaa taaaaaatta tccttcaaga ttgtcccaag aatcaacttg tcagttcatt
     4381 ttcactctgg cttaaaacaa tagttatata taaggattaa aagaagattt taacctgttc
     4441 aagaaatgct tccggtgctt caacatgtgg aagatgtttc gtttctttga gaacagaaac
     4501 ttcaatagcc gggttggagg cacaataatc atcagtgaca gcctttccgt tcggctccgt
     4561 ttcaccttca acaatataaa tactgttatc cagtgcctta agtgcatgac agatatttac
     4621 attcatatac ttagctgcaa gactggagta caaaaatctg gtataatatc cacctttatg
     4681 agctgcttca taataagcat ctgacatctg attatccaca tggaatggat tataatacat
     4741 cttttcgata aaaagattat ttatattatc acgtgatacg atcatatgat aaaccagagt
     4801 tccgaaaatc ggaatttcca gagctgcttt taacagacga tcctttctgt ttggcatctg
     4861 ttttaact
//
LOCUS       DBMDXG010000126         4857 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4254_length_4857_cov_1.710681, whole genome shotgun sequence.
ACCESSION   DBMDXG010000126 DBMDXG010000000
VERSION     DBMDXG010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4254_length_4857_cov_1.710681"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>430
                     /locus_tag="ACZPD3_04765"
     CDS             <1..>430
                     /locus_tag="ACZPD3_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1415348.1"
                     /translation="DMVDMVCSNFDNVIVVYNGANQFELGFADEYPQIKSVVWCPGTG
                     NVGFNALGKVFSGEVNPSGKTPDTFIYDMTTAPWWNNAEKTEYTNLADLAVEGMNAGT
                     AQVYAPAFTNYVEGIYVGYKYYETAAQEGAIDYDKTVQYPF"
     assembly_gap    431..440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <441..2100
                     /locus_tag="ACZPD3_04770"
     CDS             <441..2100
                     /locus_tag="ACZPD3_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1415349.1"
                     /translation="PFGYGLSYTEFEQKMGELEEKDGQISVDVEVTNTGDVAGKDVVE
                     VYYEPPYTNGGIEKSSANLIEFAKTDLLQPGESQTVTVTFSIEDMASYDENHAKAYVL
                     EKGDYAISINSDSHTVLDQKTYTADKDVVYKGENKRASDDTAATNVFEDAKGDVTYLS
                     RADHFANYEEATAAPASAELGEPYVSEYHLNSNFDKTTYLNDEDVMPTTGADNGLTLA
                     DMRDADYDDPRWEKLLDQLTVDEMANMIAMAGYQTAAMDSVGKVATLDFDGPAAINNN
                     FTGVGSIGFPIEVVVASTWNKELAQAWGECMGKISQEMGAEGWYAPGMNTHRTAFGAR
                     NYEYFSEDGVLAGNMGAKAVEGARKYGVYSYIKHFALYEGNAKMVSVWSNEQAIREIY
                     LKPFEISVKQGGANAVMVSWSFLGDKWTGESSNLMNTVLRDEWGFRGMALTDFFRNNG
                     HGFMNADAALANGVDAMLSTFNGEENNVANPEHPTAVLQMRNACKNVMYTVVSSWAYD
                     GEHEETGMENWKKAGIGIDIVIALFMAGMEVLVIRGYKKRKNVE"
     gene            2127..4565
                     /locus_tag="ACZPD3_04775"
     CDS             2127..4565
                     /locus_tag="ACZPD3_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1415350.1"
                     /translation="MKQQKLVEKMTIEEKAAILSGKTVWQTREIDRLSIPSIFLSDGP
                     HGIRKQAGAGDHLGLNASLNATCFPTAATIANSWNQDLGEEIGQALGEEAASMDVNIL
                     LGPGLNIKRSPLCGRNFEYFSEDPYLSGKMAASYIRGIQSKGVYACPKHLAVNSQELR
                     RMAMDAVVDERTLREIYLTGFEIAVKEGHAKAIMSSYNQINGIYANEDKHLLTDILRK
                     EWGFDGIVVTDWGGSNDHVKGVAAGSNLEMPSCGYDSAREVIAAVRNGTLKEADLDER
                     VGELVDAVMELTSGKKLSDKNFDRNAHHQLARKAAAESMILLKNEKQILPLDTKKSIA
                     VIGDFVFSPRYQGAGSSMVNPTKVEDMSSILQQYDLNILGMTRGYQRNGDVDENDRKF
                     ALNLAMNADIVIFCFGLDEISESEGLDRTHMRIPQNQIDLLQDISKVNENIIGILSAG
                     SAIEMPWHTCCKAILHGYLNGQAGASATLDILTGKVNPSGRLAETYPISYEQTPAFRY
                     YPSNEKTSEYRESVYVGYRYYDTSKVRVQYPFGFGLSYTEFTYSDLIIEEDGIKVSVT
                     NTGDRDGAEVVQMYVGLPNAIVFRPEKELKGFVKVYLKAGESRQLRIPFDDKTFRYWN
                     IKTGQWEIETGTYQIMVGASVADIRLTGMTERIGNSKGYPYNPAKMPYYYTGIVQKIS
                     DEEFRELLGHEIPNGRWSGNLEINDAICQMYYAKSRLARLIYRYLTKMKKKSEAQGKP
                     DLNILFIYNMPFRGIAKMTGGAVSMEMVYGIVDAVNGHFMLGVKKVVGGYFRNRRNNK
                     KYEKLLKGAGGKCR"
     gene            4562..>4857
                     /locus_tag="ACZPD3_04780"
     CDS             4562..>4857
                     /locus_tag="ACZPD3_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415351.1"
                     /translation="MNHIKNIWKCFEEKHATFAKWLYQIFYFVIFSMGVTVFQYLVFT
                     FLPGILGIGLAGTEFMWPKVSMNLFGVKFTWSLLGYNVLRDATGNILIGGGLGY"
BASE COUNT         1607 a          738 c         1232 g         1270 t
ORIGIN      
        1 agatatggta gatatggttt gctcaaactt tgataatgtg attgtggttt ataatggagc
       61 aaatcagttt gaacttggtt tcgcagatga atatcctcag attaaatctg ttgtatggtg
      121 ccccggaacg ggaaatgtgg gatttaatgc acttggtaaa gtattttcag gtgaagtcaa
      181 tccttccgga aaaacaccgg atacatttat ttatgatatg acgactgctc cgtggtggaa
      241 taatgcagaa aaaacggagt atacaaatct ggcagatttg gccgttgaag gaatgaacgc
      301 aggaactgcg caggtgtatg ctccggcatt taccaattat gtggaaggta tttatgtagg
      361 atacaagtac tatgaaacag ctgcacagga aggtgcgatt gattacgata agactgtaca
      421 gtatccattt nnnnnnnnnn tccatttgga tatggtctga gttacacaga atttgaacag
      481 aaaatgggtg aactggagga aaaagacgga cagatttctg tagatgtaga agtaaccaac
      541 accggagatg ttgccggaaa agatgtagta gaagtgtatt atgaaccgcc atatacaaat
      601 gggggaattg agaaatcttc tgcaaatctg atcgagtttg caaaaacaga tttgcttcag
      661 ccgggagaat ctcagactgt tacagttaca ttcagtatag aagatatggc ctcttatgat
      721 gagaatcatg caaaagcata tgttcttgaa aaaggcgatt atgcgatttc tatcaacagc
      781 gattcacata ctgtgctgga tcagaaaact tatactgcag ataaagatgt agtatataag
      841 ggagaaaata aaagagcatc agatgatact gcagcaacaa atgtatttga agatgcaaaa
      901 ggcgatgtga catatttatc acgagcagat cattttgcaa attatgaaga ggctacagca
      961 gcaccggcat ctgcagagct gggtgagcca tatgtttctg aatatcatct gaacagcaac
     1021 tttgataaga ctacatatct caatgatgag gatgtaatgc cgacaacggg agcagataat
     1081 ggacttacat tagctgatat gcgtgatgct gattatgacg atcctcgttg ggaaaaactt
     1141 ctggatcagc ttactgttga tgaaatggca aatatgattg caatggcagg ttatcagaca
     1201 gcagccatgg attctgtggg aaaagtggct acacttgatt tcgatggacc agcagcaatt
     1261 aataacaact ttacaggagt tggttctatt ggatttccaa ttgaagttgt ggttgcttcc
     1321 acatggaata aggaacttgc acaggcctgg ggtgaatgca tgggcaagat cagtcaggaa
     1381 atgggtgcag aaggctggta tgcaccaggt atgaatactc atagaaccgc atttggagca
     1441 agaaactatg aatacttttc agaagatgga gttcttgcag gaaatatggg agcaaaagca
     1501 gtagaaggag caagaaagta tggggtttat tcttatatca aacattttgc tctgtatgag
     1561 ggaaatgcaa agatggtaag tgtctggtca aatgagcagg caattcggga aatctatctg
     1621 aaaccatttg aaatttctgt aaaacagggt ggcgcaaatg cggttatggt ttcctggagt
     1681 ttccttggag ataaatggac tggagaaagc agtaacctta tgaatacagt tctcagagat
     1741 gaatggggat tcaggggaat ggcacttaca gacttcttcc gaaacaatgg tcatggattt
     1801 atgaatgcag atgcagcttt ggcgaatgga gtagatgcaa tgttgtccac atttaacgga
     1861 gaagagaaca atgtagccaa tccggagcat ccaacagctg tacttcagat gaggaatgcc
     1921 tgcaaaaatg tcatgtatac agttgtaagc agctgggctt atgatggaga acacgaagaa
     1981 acaggtatgg aaaactggaa aaaagcagga attggaatcg acattgtaat agcacttttc
     2041 atggcaggaa tggaagtact ggtaatcaga ggatataaga aaagaaagaa tgttgaataa
     2101 ggatgaaagt gggatggaaa acagttatga aacagcaaaa attggttgaa aagatgacaa
     2161 tagaagaaaa ggcagcgata ctcagtggaa aaactgtctg gcagaccaga gaaattgaca
     2221 ggctttccat cccgtccatc tttctttcag atggaccgca tggaatcaga aaacaggcag
     2281 gagcaggaga ccatctggga ttgaatgcat ccttgaatgc aacttgtttt cccacagcag
     2341 cgacgatagc aaacagctgg aatcaggatc tgggggaaga gattggacag gctcttggag
     2401 aggaagcagc aagtatggat gtaaatatcc tgcttggtcc gggattaaat attaaaagaa
     2461 gcccattatg tggaagaaat tttgaatatt tttcagaaga tccatatctt tccgggaaga
     2521 tggcagcgtc atatattcgt ggaatacaga gtaaaggcgt ttatgcctgc ccgaagcatc
     2581 tggcggttaa cagtcaggaa cttcggagaa tggcaatgga cgcagttgtt gacgaacgga
     2641 cattaagaga aatttatctg accggttttg agatagctgt taaagaagga cacgcaaaag
     2701 caatcatgag cagttacaac cagattaatg ggatttatgc taatgaggac aaacatcttt
     2761 tgacagatat cctgcgcaaa gaatggggat ttgatggaat tgtagtaaca gactggggcg
     2821 gaagcaatga ccatgtgaaa ggtgttgctg ccggctcaaa tctggagatg ccgtcctgtg
     2881 ggtacgattc tgcaagagaa gtgattgctg ctgtccgaaa tggaacgctg aaggaagcgg
     2941 atttggatga aagagttgga gaactggtgg atgcagtgat ggaactgaca tcagggaaga
     3001 agctttcaga taaaaacttt gacagaaatg cacatcatca actggcaagg aaagcagcag
     3061 cagaaagcat gattcttctg aaaaatgaaa aacagattct tccgctggat acaaaaaaat
     3121 ccatcgctgt tattggagat tttgtttttt cacccagata tcagggggca gggtcttcta
     3181 tggtcaatcc aacaaaagta gaagatatgt catcgatttt gcaacaatat gatctgaata
     3241 ttctggggat gacaagaggc tatcagagaa atggagatgt agatgagaat gatagaaagt
     3301 ttgcattaaa tttggccatg aacgcagata ttgtgatttt ctgctttggt cttgatgaga
     3361 tcagtgaatc agaaggactg gatagaaccc atatgcgtat tccgcagaat cagatagatc
     3421 ttctgcagga tatcagtaaa gtgaatgaaa atattattgg aatactgagt gcaggatctg
     3481 cgatagaaat gccatggcac acctgctgca aagcgatttt gcatggctat ctgaatggac
     3541 aggcaggcgc ttctgcaaca ttggatattc tgaccggaaa agtgaatcca tcaggaagac
     3601 tggcagaaac atatcccata tcttatgaac agacaccggc attccgatat tatccaagta
     3661 atgagaaaac atcggaatac cgagagagtg tttatgtggg atatcgctat tatgatactt
     3721 caaaagtccg ggtgcaatat ccatttggct ttggactttc ctatacggaa tttacatatt
     3781 ctgatcttat catagaagag gatggaatca aagtttcggt taccaataca ggtgacagag
     3841 atggagcaga ggttgttcag atgtatgtag gacttcctaa tgcaatcgtg ttccgaccgg
     3901 aaaaagaact gaaaggtttt gtaaaagttt atctaaaggc aggagaaagc agacagctca
     3961 ggattccgtt tgatgataag acattccgtt attggaatat aaaaacggga cagtgggaaa
     4021 tagaaacagg aacttatcag attatggtag gagccagtgt tgcagatatt cgtttaacag
     4081 gtatgactga gagaatagga aatagtaaag ggtatccgta taatccggca aaaatgcctt
     4141 actattatac aggaatcgtg cagaaaatat cagatgagga atttcgtgaa cttcttggcc
     4201 atgagatacc gaacgggaga tggagtggaa atctggaaat caatgatgca atctgtcaga
     4261 tgtattatgc aaaaagtcga cttgcaaggc tgatatatag atatctcaca aaaatgaaaa
     4321 agaagagtga ggcacaggga aagccagatc tgaatattct ttttatttac aatatgccgt
     4381 ttcgggggat tgccaagatg accggcgggg ccgtcagtat ggaaatggta tatggaattg
     4441 tcgatgctgt gaatggccat tttatgcttg gagtaaaaaa agtagttggc ggatatttca
     4501 gaaacagaag aaataataag aaatatgaaa aattattaaa aggagccgga gggaagtgca
     4561 gatgaatcat ataaaaaata tatggaaatg ttttgaagag aaacatgcta cttttgcaaa
     4621 atggctttat cagatattct attttgttat attcagtatg ggcgtgacag tattccagta
     4681 tcttgtattt acatttttac cagggatttt gggaattggt ctggctggta cagaatttat
     4741 gtggccaaag gtttcaatga atctgtttgg agtaaaattt acatggagct tgctggggta
     4801 taatgtattg cgtgatgcaa cggggaatat actgatcggt ggtggacttg gctatat
//
LOCUS       DBMDXG010000127         4832 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4276_length_4832_cov_1.847812, whole genome shotgun sequence.
ACCESSION   DBMDXG010000127 DBMDXG010000000
VERSION     DBMDXG010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4276_length_4832_cov_1.847812"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /gene="tig"
                     /locus_tag="ACZPD3_04785"
     CDS             <1..290
                     /gene="tig"
                     /locus_tag="ACZPD3_04785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160789.1"
                     /note="Tig; RopA; peptidyl-prolyl cis/trans isomerase;
                     promotes folding of newly synthesized proteins; binds
                     ribosomal 50S subunit; forms a homodimer; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR1415352.1"
                     /translation="FQFTGQSMDKMMEDMKPQALKRIQTRLVLEKVAEVENIQPTEEE
                     VNEEISKMAEAYKMEADKLKELLGERELEQMKKDMAVQKAVTVIADAAKEV"
     gene            386..967
                     /gene="clpP"
                     /locus_tag="ACZPD3_04790"
     CDS             386..967
                     /gene="clpP"
                     /locus_tag="ACZPD3_04790"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR1415353.1"
                     /translation="MSLVPYVIEQTSRGERSYDIYSRLLKDRIIFLGEEVNDVSAGLI
                     VSQLLFLEAEDPGKDIQLYINSPGGSVTAGMAIYDTMQYIKCDVSTICLGMAASMGAF
                     LLAGGAKGKRFALPHSTIMIHQPSGGAQGQATEIQIVADHIAQTKRTLNELLAANTGQ
                     PIEVVERDTDRDNYMTAEEAKAYGLIDGVVMHK"
     gene            1043..2359
                     /gene="clpX"
                     /locus_tag="ACZPD3_04795"
     CDS             1043..2359
                     /gene="clpX"
                     /locus_tag="ACZPD3_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR1415354.1"
                     /translation="MAEKMREGKVRCSFCQKTEDQVRKLIAGPDGKVFICDECIGICS
                     EIMEEELNPYDDEVLNSDINLLKPEEIHAVLDDYVIGQDAAKKALSVAVYNHYKRILA
                     SKNSDVELQKSNILMLGPTGSGKTLLAQTLARLLNVPFAIADATTLTEAGYVGEDVEN
                     ILLKIIQAADYDIERAQYGIIYIDEIDKITRKSENPSITRDVSGEGVQQALLKILEGT
                     VASVPPQGGRKHPHQEFLQIDTTNILFICGGAFDGIEKIIESRQDTKSIGFGAEVSVK
                     EDRNVGEILKDVMPEDFIKFGLIPEFIGRVPVVVTLDALDENALISILKEPKNSLTKQ
                     YHRLFELDGVELDFEDDALELVAKKSLERKTGARGLRAIMEGSLMDLMYKIPSDDTIR
                     KCTITKDVVDGTGEPEIVRGETPAQAKTAGSRRTSRTHKKDKPETA"
     gene            2380..4692
                     /gene="lon"
                     /locus_tag="ACZPD3_04800"
     CDS             2380..4692
                     /gene="lon"
                     /locus_tag="ACZPD3_04800"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR1415355.1"
                     /translation="MTNQNIVLPAIALRGTTILPGMIVHFDVSRERSVKAIEAAMLHD
                     QKIFLVTQIDPEVESPDLAGVYHVGTIAYIKQVVKLPQNLLRVLVEGTGRATLVKFEQ
                     EFPFIRSEITPVDEEEMQMPEPVMEAMHRSLKELFHRYCMENGKVSKELVAQILNIDN
                     VEELVEQIAVNIPLSYQNKQKILEALTLEERYEVLGAILGNEIEIMQIGRDLQKKVKA
                     RIDKNQREYILREQLKLIREELGEDNTADDAEEFKKKLQELQAGDEVKEKISKEIERF
                     KNTNSNVSENAVLRGYIETMLALPWEKKSTDSDDLKEAWKVLQEGHYGLKDVKERVME
                     FLSVRKLTHKGKSPILCLVGPPGTGKTSIARSIAEAMHKKYVRICLGGVRDEAEIRGH
                     RKTYVGAMPGRITAALQQAGVSNPLMLLDEIDKTSSDYKGDTSAALLEVLDPEQNSRF
                     MDHYIEVPQDLSEVLFIATANDVQGIPRPLLDRMELIEIAGYTENEKEHIAKEHLIPK
                     QMEENGIEKGKLTIQSAALKKIINNYTKEAGVRNLERTIGQICRKTARLIMEEDKKKV
                     TVTSKNLSDFLGKEHFNYLMANKKDEIGISRGLAWTQVGGDTLQIEVNVMPGKGELML
                     TGQLGDVMKESAQAGITYIRSIASDYKVEPEFFQENDIHVHIPEGAVPKDGPSAGITM
                     ATAILSAIIKKPVRADLAMTGEITLRGRVLPIGGLKEKLLAAKYAKIKEVLVPAENKP
                     DIQELDKEITDGLTITFVSSMKEVLNKALV"
     gene            4750..>4832
                     /locus_tag="ACZPD3_04805"
     CDS             4750..>4832
                     /locus_tag="ACZPD3_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HIR1415356.1"
                     /translation="MVIKNVSLDIVCGITSKLPDTNRPEVA"
BASE COUNT         1546 a          869 c         1250 g         1167 t
ORIGIN      
        1 acttccagtt cacaggccag agcatggaca agatgatgga agacatgaag ccacaggctc
       61 tcaagagaat ccagacaaga ctggttcttg agaaagtagc agaagttgag aacattcagc
      121 caacagagga agaagtaaat gaagagatct ccaagatggc tgaagcatac aaaatggaag
      181 ctgacaagtt aaaagaatta cttggagagc gtgaacttga gcagatgaag aaagacatgg
      241 ctgtacagaa agcagttact gttatcgctg acgctgctaa ggaagtttaa ttttaaagta
      301 atcgaatttt aagaaccgga aagagacagg ctcagatgtc tgcctctttc ggaaaaaagt
      361 cagccgcgta aatggaggcg tttatatgag tttagtacct tatgtcattg aacagacaag
      421 caggggagag agaagttatg atatctattc aaggcttctg aaggacagaa tcatttttct
      481 tggtgaggaa gtaaatgatg tgagtgcagg actgatcgta tcacagcttc ttttcctgga
      541 agcagaagat cctggtaaag atattcagct gtatatcaac agcccgggcg gttccgtaac
      601 agccggcatg gcaatctatg ataccatgca gtatattaaa tgcgatgttt ctaccatttg
      661 tcttggaatg gctgccagca tgggtgcctt tcttctggca ggcggtgcaa aaggaaagag
      721 atttgctctt ccgcattcca ctatcatgat ccatcagcca tccggcggtg cgcagggaca
      781 ggcaacagag atccagatcg tggcagatca tatcgcacag acaaagagaa ctttaaatga
      841 gttattggct gcaaataccg gtcagccgat agaagttgtt gagcgtgata cagaccggga
      901 caactatatg actgctgaag aagcaaaggc atatggactg attgacggtg tagtcatgca
      961 taaataatgc gaaagataag actcctgtga atcgtcacag gagtcttatt agttagtata
     1021 gagaaaggac agacataaat tcatggcaga aaaaatgaga gaaggaaaag taagatgctc
     1081 cttttgccag aagacagaag atcaggtaag aaaactgatc gcaggtccgg atggtaaagt
     1141 atttatatgc gacgagtgca ttggcatctg ctcagagatc atggaggaag aattaaatcc
     1201 ttatgatgac gaggttttga attctgatat taatctgtta aaaccggaag agatccatgc
     1261 agttttggat gattatgtaa ttggtcagga tgcagcaaaa aaggctcttt ccgttgctgt
     1321 ttataatcac tataaaagaa ttcttgcgtc aaaaaattct gatgtagagc ttcagaagag
     1381 taatattctg atgcttggac ctacaggttc aggtaaaact ctgctggctc agacacttgc
     1441 cagacttctg aatgtaccgt ttgctattgc agatgctacc acactgacag aagccggata
     1501 tgtaggagag gatgttgaga atattcttct caagatcatt caggcagcag attatgatat
     1561 tgaacgtgca cagtacggaa ttatatatat tgatgagatt gataagatca caagaaaatc
     1621 tgagaatcca tccataaccc gcgatgtttc cggtgaaggc gtgcagcagg cacttctgaa
     1681 gattctggaa ggtacagtgg caagtgttcc gcctcagggt ggccgcaagc atcctcatca
     1741 ggagtttctt cagattgata caacaaatat tctgtttatc tgcggcggtg cttttgacgg
     1801 aatcgagaag atcattgagt ccagacagga taccaagtcc atcggtttcg gtgcagaggt
     1861 ttctgttaag gaagacagaa atgtaggtga gatcctgaag gatgtaatgc cggaagactt
     1921 tatcaaattc ggtctgattc ctgagtttat cggtcgtgtg ccggtagttg taactctgga
     1981 tgcgctggat gagaatgcgc tgatcagtat tctgaaagag ccgaagaact cacttaccaa
     2041 acagtatcac agattatttg aactggatgg tgttgaactt gattttgaag atgatgcact
     2101 tgaactggtc gcaaagaaat ctctggaacg taagacagga gccagaggtt tgcgtgcgat
     2161 catggaaggt tcactgatgg atctgatgta taagattccg tcagatgaca cgatccgcaa
     2221 gtgtacaatt accaaagatg tagtagatgg caccggtgag ccggagatcg tccgcggaga
     2281 aactcctgca caggcgaaga cagccggaag cagaagaacg tcccggacac acaagaagga
     2341 taaaccggaa acagcctgat gaaagggact gaacaataaa tgacaaatca gaatatagtg
     2401 ctgcctgcga tcgctctcag ggggactacc atccttcccg ggatgatcgt tcattttgat
     2461 gtgagcaggg agcgttctgt caaggcgatt gaagctgcca tgcttcatga ccagaagatt
     2521 tttctggtta ctcagattga tcctgaggtg gaatcaccgg atctggcagg tgtatatcat
     2581 gtaggaacca ttgcctatat caaacaggtt gtgaaacttc cgcagaatct tttgcgggta
     2641 cttgtagagg gaacgggaag agccacactt gtgaaatttg agcaggagtt tccgtttatc
     2701 agaagtgaga tcacacctgt tgatgaagaa gaaatgcaga tgccggaacc agtgatggaa
     2761 gccatgcaca gaagccttaa ggaattattc cacagatact gcatggaaaa tggaaaagtc
     2821 agcaaagagc tggtagcaca gattttaaat atagataatg ttgaggaact ggttgagcag
     2881 attgccgtaa atattccttt atcttatcag aataagcaga agattctgga ggcacttaca
     2941 ctggaggaac gttatgaagt tctgggtgca atccttggaa atgagattga gatcatgcag
     3001 attggcagag atctccagaa aaaagtgaag gccagaattg ataagaatca gagagaatat
     3061 attctcagag agcagctgaa actgatacgt gaggaactgg gtgaggataa tactgcggat
     3121 gacgcagagg agtttaagaa gaaactccag gaacttcagg ccggcgatga ggtaaaagag
     3181 aagatcagca aagaaatcga gagatttaag aataccaaca gcaatgtatc tgagaatgca
     3241 gtactgcgtg gttatattga gaccatgctg gcacttccgt gggagaagaa atccacagat
     3301 tctgatgatc tgaaagaagc atggaaagta cttcaggaag gtcattacgg tctgaaagat
     3361 gtgaaggagc gtgtgatgga gttcctttcc gtccgcaaac tgacacataa gggaaaaagt
     3421 ccgatccttt gtctggtagg acctcccgga acaggtaaaa cctccattgc ccgatcgatt
     3481 gcagaagcaa tgcataagaa atatgtgcgt atctgtctgg gcggtgtgag agatgaggct
     3541 gagatccggg gccacagaaa gacttatgta ggagcaatgc cgggaaggat cactgcagca
     3601 ttacagcagg caggagtgag caatcctctg atgcttctgg atgagattga caagaccagc
     3661 agtgattata aaggagatac ttctgcggca cttcttgagg tgcttgatcc ggaacagaac
     3721 agcagattta tggatcatta tatagaggtt ccacaggatc tttcagaagt actgtttatt
     3781 gcaactgcaa acgatgttca gggcattccg agaccacttc ttgaccgtat ggaactgatc
     3841 gagatcgcag gttatactga gaatgagaag gaacatattg caaaggaaca tctgattccg
     3901 aagcagatgg aagagaacgg tatcgagaaa ggtaaactga caatccagtc agcggctctt
     3961 aagaagatca tcaacaatta tacaaaagaa gccggagttc gaaacctgga gagaaccatc
     4021 ggacagatat gcagaaaaac tgcacgtctg atcatggaag aagataaaaa gaaagttaca
     4081 gtgacttcaa agaatctgtc agattttctt ggaaaagagc attttaacta tcttatggca
     4141 aataagaaag atgagattgg tatttcccgt gggcttgcct ggacacaggt gggcggcgat
     4201 acactgcaga ttgaagtcaa tgtaatgccc ggcaagggtg agctgatgct taccggacag
     4261 ttgggagatg ttatgaaaga gtctgcacag gcaggtatta cttatattcg ttccattgct
     4321 tctgactata aagttgagcc ggaatttttc caggaaaacg atattcatgt gcatattccg
     4381 gagggggcag taccaaaaga cggaccatcc gcaggtatta ccatggcaac tgcaatcctt
     4441 tccgcaatta ttaagaagcc ggtaagggca gaccttgcaa tgaccggtga gatcacactg
     4501 agaggaagag ttcttccgat cggcggtttg aaagaaaaac ttcttgcggc aaaatatgct
     4561 aagatcaaag aggtgttagt gccggcagaa aacaaaccgg atattcagga attagataag
     4621 gaaatcacag acggacttac cattaccttt gtaagttcga tgaaggaagt gcttaataag
     4681 gcactggtat aaaataaatg gtcagtcagt aaagattgac catttgttgt gaaaaaagag
     4741 aggaaatcaa tggttattaa gaatgtatca ttagatatcg tatgcggtat taccagcaaa
     4801 ctcccggaca ccaacagacc ggaggttgca tt
//
LOCUS       DBMDXG010000128         4805 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4293_length_4805_cov_1.944728, whole genome shotgun sequence.
ACCESSION   DBMDXG010000128 DBMDXG010000000
VERSION     DBMDXG010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_4805_cov_1.944728"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            328..1515
                     /locus_tag="ACZPD3_04810"
     CDS             328..1515
                     /locus_tag="ACZPD3_04810"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR1415357.1"
                     /translation="MIAEKMKPYVKNNSAIRMMFEEGNRLRAIYGADKVYDFSLGNPS
                     VPAPECVKEAIIDLVNEVEPTVLHGYMSNAGFEDVRQTIAESLNHRFGTKFAAKNLIM
                     TVGAASGLNVIFKTILNPEEEVIVFAPYFLEYGAYVRNFDGKLVEISPDTTTFQPNLE
                     EFEQKITAKTRAVIVNTPHNPTGVVYSEETIKKLAAILEKKQQEFGSVIYLISDEPYR
                     ELAYDGVEVPYLTKYYDNTIVGYSYSKSLSLPGERIGYLVIPDEADGSEELITAAAIA
                     NRTIGCVNAPSLMQKVIAKCVDAEVDVAAYDKNRLALYNGLKECGFECIKPQGAFYLF
                     VKSPVADEKAFCEAGKKYNILMVPGSSFACPGYVRLAYCVSYDTIINSLPEFKKLAEE
                     FGL"
     gene            1512..2864
                     /locus_tag="ACZPD3_04815"
     CDS             1512..2864
                     /locus_tag="ACZPD3_04815"
                     /EC_number="2.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050277 - sedoheptulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sedoheptulokinase"
                     /protein_id="HIR1415358.1"
                     /translation="MKTAGIDIGTTTISGVVLEKEENGQAKILEAKTVENGCFIETGN
                     EWERIQYAKEIVERAVNLLDYFLEKYPHVERIGLTGQMHGIVYVDKEGNCVSPLYTWQ
                     DARGSICDGDQIPLTEEIRERCQIHAASGYGLVTHIYNIRHNLVPDSALSFCTIMDYF
                     GMYLTGRKNPLIHVSNAAGLGFFDSRKMCFEKEKLAEMGVDVNWLPDVCTEIEKLGTY
                     RGRTVTTAIGDNQASFLGAAGDEENTLLVNMGTGGQISVLSGQYFSGDGIEARPFLNG
                     KYLLAGASLCGGKAYALLEQFFRKIVKEATGQEQPLYKVMEKMARTGRDQNSERTESR
                     KIKVETTFDGTRVNPEKSGSITQVYSENFTPEDFCYGVLKGMSTELYQMYMTIQKGTG
                     IKIRRMIGSGNGLRKNPVLCEIIADMFKAELVLAECEEEAATGAAMSSSMYNYINSPR
                     "
     gene            2889..3119
                     /locus_tag="ACZPD3_04820"
     CDS             2889..3119
                     /locus_tag="ACZPD3_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415359.1"
                     /translation="MNNQLILILEFEERGCPENRQLLLYCPIYCMLFFDMFRYILEHA
                     GKFYENCKKNQEKKDSILNAMEKMQFLKKQLL"
     gene            3266..>4805
                     /locus_tag="ACZPD3_04825"
     CDS             3266..>4805
                     /locus_tag="ACZPD3_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR1415360.1"
                     /translation="MGIFSMILSLLSGVALFLFGMSLMGDGLKMVAGNKLEAFLYRMT
                     NTPLKGVALGTGVTSVIQSSSATTVMVIGFVNSGMMKLKQAIGIIMGANIGTSITGWI
                     LCLSYIDGKNGIAKILSTATISAVVAIIGIILRMACKRSVHKNIGNIMLGFAILMTGM
                     QTMSGAVSPLKDSPTFTNMLTMFSNPLIGILVGIAFTAVLQSASATVGVLQALSVTGI
                     LTFSSAFPIVLGIGVGAACPVLISAIGANKNGKRTALVYLINDLFGLILWSVIFYTVN
                     AVVHFGFMDMIMSPVAIALLNTVFRVATVCVLFPFIPKIEQLVCWLVKDSAEELEDEA
                     DFDLLEERLLNYPALAIGQCHRAMSGMARKLRKNVNRAMNLLNEYQQDKFDKVQRKEN
                     LIDKYESRLGEYLMKLTKHEMNSAQTRQASLYLHTINDFERIGDHASYIAYMSSEMHD
                     NHTNFSQEAWDELNVVMEAVREEINLTCRAFLNDDKEMAQRVAPLGMIITSLCNELKM
                     HHVER"
BASE COUNT         1459 a          840 c         1192 g         1314 t
ORIGIN      
        1 aaattaacta tacgttctat tctttttgcc ttttttgaaa aaagtgatta aaaaactatt
       61 gacattttgt tactggagga gcgcaaagat tccttctgat acaagcaagt tcccaccccc
      121 tgcttattgc ttttcgcaat taaagcaggg gacttacttg acttcggcta aaaagatgga
      181 atatagtata ttgtcgcaca ttgacacttt accatgataa ggttaaaata aagataaatt
      241 atgcactgca agagaaattg gacagttgca atattattct gaatttcggc ggtttgccgg
      301 gattatttgg acaggagcga atatgttatg atagcagaaa aaatgaaacc ttatgtaaaa
      361 aataattcag ccatccgtat gatgtttgag gagggaaatc gtctcagagc catttacggt
      421 gcggacaaag tttatgattt cagtcttggg aatccgagcg ttccggcgcc ggagtgtgtg
      481 aaggaggcta ttattgatct ggtgaatgaa gtggagccga cagtgctgca tggttatatg
      541 agtaatgcag gatttgagga tgtgcgccag acgattgcgg aatctctgaa ccaccgcttt
      601 ggaactaaat ttgcagcgaa gaatctgatt atgacagttg gagcagccag tggtctgaat
      661 gtgattttta agacaatcct gaatcctgag gaagaagtga ttgtttttgc accgtacttt
      721 ctggaatatg gtgcatatgt gcgcaatttt gatggcaagc ttgtggaaat ctctccggat
      781 acaaccacat tccaaccgaa tcttgaggag tttgagcaga agattacagc aaagacaaga
      841 gcagtgatcg taaatacacc tcacaatccc actggtgttg tatattccga agaaaccatt
      901 aagaagctgg ctgcaattct tgagaaaaag cagcaggaat tcggcagtgt gatttatctg
      961 atctctgatg agccatatag agagctggct tatgacggtg tagaggttcc gtatttgaca
     1021 aaatattatg acaataccat tgttggctat tcttacagta aatctctgtc ccttccggga
     1081 gagcgtatcg gttatctggt aattccggat gaagcagatg gcagtgaaga acttatcact
     1141 gctgcggcta ttgccaaccg tactatcgga tgtgtcaacg ccccatccct gatgcagaag
     1201 gtgattgcca aatgtgtaga tgcagaagtg gatgtagctg catatgacaa gaaccgtctg
     1261 gcattatata acggactgaa ggaatgcgga ttcgagtgca ttaaaccgca aggcgcattc
     1321 tacctctttg tgaaatcacc ggtagcagat gaaaaagcgt tctgcgaagc tggaaagaaa
     1381 tataatattc tcatggttcc gggaagttcc tttgcctgcc cgggttatgt gagactggct
     1441 tattgtgtat cttatgatac tattattaat tccctgccgg agtttaagaa actggcagaa
     1501 gagtttggac tgtgaaaaca gcggggattg acattgggac aacaacgata agcggtgttg
     1561 ttctggaaaa agaggaaaac ggacaggcaa agattctgga ggcaaaaact gtagagaacg
     1621 gttgctttat cgaaaccgga aatgaatggg aaagaataca gtatgcaaaa gaaattgtag
     1681 aaagagcagt gaatctgctg gattatttcc tggagaagta tccgcatgtt gagcggattg
     1741 ggctcacagg gcagatgcat ggaattgtct atgttgacaa agaaggaaat tgtgtcagtc
     1801 cgttatatac atggcaggat gccagaggaa gtatctgcga tggggatcag attcctttaa
     1861 cagaagaaat ccgggaaaga tgtcaaatcc atgctgcttc aggatatggg cttgttacac
     1921 acatttataa tatcaggcat aatctggttc cggattcggc gttgagtttc tgtacaatca
     1981 tggattattt tggaatgtat ttaacgggac gaaaaaatcc tctgatacat gtaagcaatg
     2041 cagcaggttt gggattcttt gacagtcgta agatgtgctt tgagaaagaa aaactggctg
     2101 aaatgggagt ggacgtaaac tggctgccgg atgtctgtac ggaaatagaa aaactgggta
     2161 catacagagg aaggacagtt acaacagcaa tcggagacaa tcaggcaagc ttccttggag
     2221 cagcaggtga tgaggagaat acattactgg tcaatatggg aaccggtgga cagatttctg
     2281 ttctttccgg tcagtatttt tcgggagatg gaattgaggc gagacctttc ctgaatggga
     2341 agtatctgct tgcgggagca tctctgtgtg gtggcaaggc gtatgcgctc ttggaacagt
     2401 ttttccggaa aattgtaaaa gaagcgaccg gacaggagca gccattatac aaagtaatgg
     2461 aaaagatggc aagaacaggg agagaccaga atagtgaaag aactgaaagc cgaaagatta
     2521 aagttgaaac aacatttgat ggtacgcggg tgaatccgga aaaaagcggc agtattactc
     2581 aggtatattc cgaaaacttt acacctgaag atttctgcta tggtgttctg aagggaatga
     2641 gcacagaatt gtatcagatg tatatgacta ttcagaaagg aactggtata aaaatcagac
     2701 gcatgattgg ttcagggaat ggactgagaa aaaatccggt actgtgtgaa attattgcgg
     2761 atatgttcaa ggcagaactt gttctggcag aatgtgaaga ggaagctgcg acaggggcag
     2821 caatgagcag tagtatgtat aactatatca attcccccag ataataaaat gaagcagtga
     2881 agcggattgt gaataaccaa ttgattttaa tactggaatt tgaggaaagg ggctgtcctg
     2941 aaaatagaca gctccttctt tactgtccga tttattgtat gttatttttc gacatgttca
     3001 gatatatttt ggagcatgcc ggaaaatttt atgaaaactg caaaaaaaat caggaaaaaa
     3061 aagacagcat tttaaacgct atggaaaaaa tgcaattttt gaaaaaacaa ttattgtaat
     3121 aatctcccgg acgtgacata ataagaatgg aaaagtaaaa agcaggttaa attctgatta
     3181 gaaacaggta aaataacctg cttacattat gcgccgattt tctgagatga ggtgggaaat
     3241 caggcagacg acaaaggaga aaataatggg aattttttca atgatattgt cattactgag
     3301 cggagtagcg ttattcctgt tcggtatgtc attgatgggc gacggcctga aaatggttgc
     3361 cggaaataag ctggaagctt tcctgtatcg aatgacaaac acaccgctga agggagttgc
     3421 tctgggtact ggtgttacca gcgtgattca gtcttcttct gccacaaccg ttatggtcat
     3481 tggttttgtt aactcaggta tgatgaaact gaaacaggcc atcggtatta tcatgggagc
     3541 caatatcgga accagtatta ccggatggat cctctgtctt tcttatattg acggaaagaa
     3601 cggaattgcg aagattttat ctacagcaac aatttcagca gtagtagcga ttattggtat
     3661 tatccttcgc atggcatgta agcgttccgt gcataaaaat atcggtaata tcatgcttgg
     3721 ttttgcaatc ctgatgacag gtatgcagac aatgagcggt gctgtttcac cactgaagga
     3781 cagccccaca tttacaaaca tgctgacaat gttttccaac ccgctgatag gtattctggt
     3841 aggtattgca tttaccgcag tgctgcagag tgcatctgcc acagtcggtg tattgcaggc
     3901 gttatctgta acaggtatcc tgacattctc cagtgcattc ccgatcgttc ttggtatcgg
     3961 tgtaggtgct gcctgtccgg ttctgatttc agcaatcggt gcgaataaga atggtaaacg
     4021 tacagcactt gtttatctga tcaatgacct gtttggtctt atcctctggt cagtgatctt
     4081 ctacactgta aatgctgttg tgcatttcgg atttatggat atgattatgt caccggttgc
     4141 aattgcttta ctgaatactg tattccgtgt agcaacagta tgtgttcttt tcccgttcat
     4201 accgaagatc gagcagctgg tgtgctggct tgtaaaagac agtgcagagg aactggaaga
     4261 tgaagcagac tttgatcttc tggaagaacg tcttctgaat tatccggcac ttgctattgg
     4321 acagtgtcat cgtgccatga gcggtatggc aaggaagctt cgcaagaatg tcaaccgtgc
     4381 catgaacctt ctgaatgaat atcagcagga taaatttgat aaggtacaga gaaaagagaa
     4441 cctgatcgat aaatacgaaa gccgtcttgg ggaatatctg atgaagctca caaagcatga
     4501 gatgaacagt gcccagacaa ggcaggcatc cctttatctt cataccatca atgactttga
     4561 gagaatcggt gaccatgcat cctacattgc ctatatgtcc agtgaaatgc atgataatca
     4621 cactaatttc tcacaggaag cctgggatga gctgaatgta gtaatggaag cagttcgtga
     4681 ggagatcaac cttacctgtc gtgcattcct caatgatgac aaagagatgg cacagagagt
     4741 tgcaccgctt ggaatgatta tcacatcctt atgcaatgag ctaaagatgc atcatgtaga
     4801 gcgtc
//
LOCUS       DBMDXG010000129         4763 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4333_length_4763_cov_1.753968, whole genome shotgun sequence.
ACCESSION   DBMDXG010000129 DBMDXG010000000
VERSION     DBMDXG010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4333_length_4763_cov_1.753968"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            216..1187
                     /locus_tag="ACZPD3_04830"
     CDS             216..1187
                     /locus_tag="ACZPD3_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415361.1"
                     /translation="MGKRCQVCDGPIVNGRCKYCGMPYRNDMELYHLNEDRSEHYRHA
                     SAKVKKAMAENEIPLSDRNKTVPKAAKTSSARTQTIRTQNTKVQTSRIQTSGTKKTSG
                     QSYSAQTARTYSTGKKTAKSEKKKSGKAGKIFWIIVLILMVIIGQMAENWDTIGYRIE
                     EFIYDEFDIDLNSIFDDSSSDEVNESNKNNESNENNWTDDWSETISAVKNGQDEQRGN
                     PDWYMFTGENYIVRTDDTASEIGDTDASDQGYDFTIDPGEYRIVSGWEKVALEIRSSS
                     GKDETVKFDEADHEEKIELHAGDEVSVVSLDGQDNYLAMYQIQQYDE"
     gene            1308..3443
                     /gene="nrdD"
                     /locus_tag="ACZPD3_04835"
     CDS             1308..3443
                     /gene="nrdD"
                     /locus_tag="ACZPD3_04835"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1415362.1"
                     /translation="MIYVIKKDGTREEFNSQKIVAAVNKSAARILYTFSDKEKEFICQ
                     FAEEHAEALGKNEIEIQEMHNIVEGALERVNPAVAKSYRDYRNYKLDFIHMMDDVYTK
                     SQAIRYIGDKSNANTDSALVATKRSLIFNELNKELYRKFFMNRNELQACKDGYIYIHD
                     QSARLDTMNCCLFDVASVLSGGFEMGNVWYNEPKTLDTAFDVMGDIILSTAAQQYGGF
                     TVPEVDKILAPYAQKSYDKYIQEFMKYSDESWTGREERAIEYALDKVRRDFDQGWQGI
                     EYKLNTVGSSRGDYPFVTVTLGLGIKQFEKMASISLLEVHKGGQGKAGRKKPVLFPKI
                     VFLYDENIHGKGKISEDVFEAGVDCSAKTMYPDWLSMSGKGYISSMYKQYGKVISPMG
                     CRAFLSPWYERGGMYPADDKDVPVFVGRFNIGAVSLHLPMILAKARAESRDFYEVLDF
                     YLEMIRNLHIRTYDYLGQMRASTNPLAYCEGGFYGGHLKPNEKIGKILKPMTASFGIT
                     ALNELQELYNGKSIAEDGQFALDVLKYINRKVNEFKEKDGYLYAIYGTPAESLCGLQV
                     EQFRKMYGIVKGVSDRPYVSNSFHCHVTEDVTPIEKQDLEGRFWELCNGGKIQYVRYP
                     IDYNREAIRTLIRRAMSLGYYEGVNLSLAYCDDCGHEELEMDVCPVCGSKNLTKIDRM
                     NGYLSYSRVHGDTRLNEAKMAEIAERKSM"
     gene            complement(3607..4539)
                     /gene="arcC"
                     /locus_tag="ACZPD3_04840"
     CDS             complement(3607..4539)
                     /gene="arcC"
                     /locus_tag="ACZPD3_04840"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - L-arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR1415363.1"
                     /translation="MSEKITVVALGHRALGTTLPEQKIATKKAAKAVADLVEAGNRVV
                     ISHSNGPQVGMIHTAMNEFGKTHPDYTFAPMSVCSAMSQGYIGYDLQTAIRAELISRG
                     IYKPVATILTQVVIDPYDDAFAEPEKIIGRVLTEEEAETEESKGNFVTKLADGTYKRI
                     LAAPKPQKIVELETIRTLVDAGQVVIAAGGGGIPVMEQGIDLHGASAIIEKDLSSGLL
                     AQELNADTLLILTSVEKVSLNLGQDNEEYLNTISVDDAKKYMADNQFAPGSMLPKFEA
                     GISFIEKGDNRRTIITDIAHAKDGYFEKTGTIIK"
BASE COUNT         1480 a          834 c         1133 g         1316 t
ORIGIN      
        1 aatatccatt atcttttccc acgctattat aacaggaaaa tggtagtttg acaattatta
       61 ctgttcattt ggaagtatct cgcgggatat tatggttacg gagaacagaa cgaacagtaa
      121 cacaatttca gctttgcaag attggggcga aatgttaaaa taaatgtgta aatgaatgaa
      181 gcgttaaatt tatataaaac tcagggggaa gatatatggg aaaaaggtgt caggtctgtg
      241 atggaccaat tgtaaacggc aggtgtaaat attgtggaat gccatataga aatgatatgg
      301 aactgtatca tctgaacgaa gaccgttcag agcactatcg tcatgcttcg gcaaaggtga
      361 aaaaagcaat ggcagagaac gagatccctt tgtctgacag aaataaaaca gttcccaaag
      421 ctgcaaaaac ttcgtctgcc agaacacaga ctattagaac gcaaaataca aaagtacaaa
      481 cttcaagaat acaaacatca gggacaaaga aaacatcagg tcagtcgtac agcgcacaga
      541 ctgcccgtac atacagcacc ggaaaaaaga cagcaaaaag tgagaagaaa aagagcggca
      601 aggcaggcaa aattttctgg atcattgtgc tgattctgat ggtaataata ggtcagatgg
      661 cagagaattg ggatacgatt ggatacagga tagaagaatt tatctatgat gagtttgaca
      721 tagatctcaa cagtattttt gatgacagca gttctgatga agtaaatgag agtaataaga
      781 ataatgaaag taatgagaat aactggacag atgactggtc tgagactatc agtgcagtaa
      841 agaacggaca ggatgaacag aggggtaatc cggactggta tatgttcacc ggagagaatt
      901 atattgtcag aacggatgat actgcatcag agattggcga tacagatgcc agtgaccagg
      961 ggtatgattt tacaatagat ccgggagaat acaggatagt gtccggctgg gagaaggttg
     1021 ctctggaaat cagaagttct tcaggaaaag atgaaactgt aaaatttgac gaagcagacc
     1081 acgaggagaa aatagaatta catgccgggg atgaggtatc tgtggtatct ctggatggtc
     1141 aagataatta tcttgccatg tatcaaattc agcaatatga cgaataattt tttgcccctt
     1201 tctttttgtc agagactgtg ctatacttgt taaatgtacc acatatagag agaattgtgg
     1261 tgaatgaaag aaaaaaatac tatatattgt atatgggagc aaaaggtatg atatatgtta
     1321 taaagaaaga tggtacgaga gaagagttta attcacagaa gatcgtagca gcagtaaata
     1381 agtcggcagc cagaatttta tatacatttt cggataaaga gaaggaattt atctgccagt
     1441 ttgcggaaga acatgcggag gcactgggaa agaatgagat agagatccag gagatgcaca
     1501 acatcgtaga gggagcactt gagagagtga acccggcagt ggctaagagc taccgcgatt
     1561 acagaaatta caaacttgac tttatccata tgatggatga tgtatatacc aagagtcagg
     1621 caatccgtta tattggtgat aagagtaatg ccaatacaga cagtgcgctt gtagctacaa
     1681 agcgaagcct gatctttaat gaactgaaca aagagcttta tcgtaaattt tttatgaatc
     1741 gtaatgaact tcaggcatgt aaagatggtt atatttatat tcatgaccag tctgcacgtc
     1801 tggataccat gaactgctgt ctctttgatg tggcatctgt actcagcggc ggatttgaga
     1861 tgggaaatgt atggtacaat gagcctaaga cactggatac tgcgtttgat gttatgggtg
     1921 atattatcct gagcactgct gcacagcagt atggcggatt cacagtaccg gaagtagaca
     1981 agatccttgc accttatgca cagaagagtt acgataaata tatccaggaa tttatgaaat
     2041 actctgacga gagctggaca ggacgtgaag aacgtgcgat cgagtatgcg cttgacaaag
     2101 tccgcagaga ttttgaccag ggatggcagg ggattgagta taagctgaat acagtcggat
     2161 cttccagagg ggattatcca tttgtaactg taactcttgg acttggaata aagcagttcg
     2221 agaagatggc aagtatttct cttcttgaag tgcataaagg cggacagggt aaggcaggac
     2281 gcaagaaacc ggtactcttc ccgaaaatcg tattccttta tgatgaaaat atccatggaa
     2341 aaggcaagat cagtgaggat gtatttgagg caggggttga ctgttctgcc aagactatgt
     2401 atccggactg gctgtccatg tcaggaaaag gatatatttc cagtatgtac aagcagtatg
     2461 gaaaggttat aagtcctatg ggatgtcgtg cgttcttaag cccctggtac gagagaggcg
     2521 gtatgtatcc ggcagatgat aaggatgttc ctgtgtttgt gggaagattt aacatcggtg
     2581 cggtaagcct tcatcttcca atgatccttg ccaaagccag agcagagagc agggatttct
     2641 acgaagtgct ggatttctat cttgagatga tccgtaatct gcatatccgt acctatgatt
     2701 atctgggaca gatgcgtgcg tcaaccaacc cgctggcata ttgtgaggga ggattttacg
     2761 gtggacatct gaaaccgaat gaaaagattg gcaagattct gaaaccaatg acagcatcct
     2821 tcggaatcac tgcattaaat gaactccagg aactttacaa tggcaaatcc attgctgaag
     2881 acgggcagtt tgcactggat gtgctgaaat atatcaacag gaaagtaaat gaatttaaag
     2941 agaaagatgg ttatctttat gcaatttatg gaacaccggc agagagttta tgcggactgc
     3001 aggtggaaca gttccgtaag atgtatggca ttgtgaaagg ggtttctgat cgtccttatg
     3061 taagcaacag tttccactgt catgtgactg aagatgtaac tccgattgaa aaacaggatc
     3121 tggagggacg tttctgggag ttgtgcaacg gcggtaagat tcagtatgta cgttatccga
     3181 tcgattataa tcgtgaagca atacgcactc tgattcgcag agccatgtct ttgggatatt
     3241 atgagggagt aaatctttct cttgcttact gtgatgactg cggacatgag gaacttgaaa
     3301 tggacgtgtg tccggtctgc ggttccaaga atctgacaaa gattgaccgt atgaacggat
     3361 atctttccta cagccgtgtg catggtgata ctcgtctgaa cgaagcgaag atggcagaga
     3421 ttgcggaacg taaatctatg taatatcagg tcaggggtaa acttttgaat ctgacatcat
     3481 aatatgataa ttaaaagaca tataccggca agatgatatg tattgctctg gtatatgtct
     3541 ttttgtattg tcagataaaa tatagcaatc ctcttaaatg tattatttac gaggattgct
     3601 atagaatcat ttaatgatag ttccggtttt ttcgaaatat ccatcctttg catgcgcaat
     3661 atctgtgatg atcgttcttc tgttatctcc cttttcgata aaagagattc cggcttcgaa
     3721 tttaggaagc atggagcctg gggcaaactg attgtcagcc atatatttct tagcatcatc
     3781 tacagaaatt gtattaagat attcttcatt gtcctgaccg aggttcagac ttactttctc
     3841 aacactggta aggatcagaa gagtatctgc atttaattcc tgtgcaagaa gtccgctgga
     3901 aaggtctttc tcaatgatcg cacttgcacc atgaagatcg attccctgtt ccataaccgg
     3961 gattccgccg ccgcctgctg cgattactac ctggcccgca tctacaagtg tgcggattgt
     4021 ttcaagctct acgattttct gcggtttcgg agctgccagg attcttttat aagtgccgtc
     4081 agccagttta gtgacaaagt ttcccttgct ttcttcagtt tcagcctctt cttctgtaag
     4141 gacacgtccg atgatcttct caggttcagc gaaagcatca tcatatggat caatgacaac
     4201 ctgtgtaagg atagtagcta ccggtttata aataccgcgg gaaataagtt ctgcgcggat
     4261 tgcggtctgg aggtcatatc cgatatagcc ctgactcatt gccgaacata cggacatagg
     4321 tgcaaatgta tagtcaggat gtgttttgcc aaattcattc atggctgtgt ggatcattcc
     4381 aacctgtgga ccgttactgt gggaaatgac tacacgattt ccggcttcta caagatctgc
     4441 gacagctttg gctgcttttt ttgtagcgat tttctgctcc ggtaaggtgg ttcctaatgc
     4501 tctgtggccc agtgcaacta ctgtaatttt ttcgctcatg ggaattacct cgattctttc
     4561 atgttctgta aatatgatat ctgcggggtg atcttatata taaagatttt tgaaattttt
     4621 tatcactgat atcataaata ttcaatcata ttgatatcct acattataaa agactgccag
     4681 tagaattgca atagaaaata actcaaaaaa cacctcgcgg gatattacca gtgaacagta
     4741 acaaaaaaca aacagctctc aca
//
LOCUS       DBMDXG010000130         4755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4343_length_4755_cov_1.778040, whole genome shotgun sequence.
ACCESSION   DBMDXG010000130 DBMDXG010000000
VERSION     DBMDXG010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4343_length_4755_cov_1.778040"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="ACZPD3_04845"
     CDS             complement(<1..1223)
                     /locus_tag="ACZPD3_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR1415364.1"
                     /translation="MKLNFEYGQGTMAAELPDNTDVFIPGETVKDPAYIPEDQLEAAY
                     LESLAHPIGMPTLTELAGPGKTVTIVVPDRVKGGEQATSHRKLSIKYILKELYAAGIE
                     KKDILFIISNGLHPRSTEADAKAIFGEELFNEFWHTGQIISHDSEDQEHMVYLGTTHR
                     GDPVYMNKYVFECDIPILIGHVQGNPYGGYSGGYKHSATGITNWRCIASHHVPSVMHR
                     DDFTPVNGGSLMRNKFDEISMHMEEKMGHPFFCCDAVLDTQSRQIAIYSGYAKEMMPI
                     SWKLADKRTYVHWAEKKYDVLVFGMPQNFHYGDGMGTNPIMMMQALSAQVLRFKRVMS
                     DNCVIICSSICNGYFHDERWPYLRELYDLFQHDHMNTLPDMNRLGEYFATNEEYIRKY
                     RYTNAFHPFHGFSMMS"
     gene            complement(1250..2098)
                     /locus_tag="ACZPD3_04850"
     CDS             complement(1250..2098)
                     /locus_tag="ACZPD3_04850"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase family protein"
                     /protein_id="HIR1415365.1"
                     /translation="MKHIYLNLKRFDVPVEFGGVNRLAPVADWASCIVKNTQEELKTY
                     DPTEVEFGMYFPEVHLLNAVAAKTEGSPVQIGCQSVFRADTAVGGNFGAFTTNRPASA
                     MVAAGCETTIIGHCEERNDKAGILAEAGVTDTDAVNRLLNQEIKCAIERGMKVLYCIG
                     EKSEEQEQWQEVLGKQLEIGLKDVDTSKVVIAYEPIWSIGPGKTPAGKEYITKIAKFV
                     KEKTNGMDIVYGGGLKQDNAEMLASIDEIDGGLIALTRFQGEIGYYPEEYLEIIRLYM
                     GRENNK"
     gene            complement(2185..2955)
                     /locus_tag="ACZPD3_04855"
     CDS             complement(2185..2955)
                     /locus_tag="ACZPD3_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014035.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR1415366.1"
                     /translation="MKWQNAIVRDFDQKGYVNEMYILTALVAFAGSFVQSSSGFGYAI
                     ICMAIWPMFMPFYTASILEAIAAFFMVIYIAVRLWKFIDWKLLLPPVIMATVTSFLGV
                     NTLMALDEDVLSKIMGTVLLILAVYFIFFSSKVHLKASLVSGLVAGAISGFCGGLFNI
                     GGPPMVAYFLSVTDDKEKYNATLQMFFTINTISIFLIHVFQGHVSGSMLPLVGAALAG
                     TAVGTAGGMFLFRRLTMAGIKKTVYIFMICAGSYLLLS"
     gene            complement(2921..4075)
                     /locus_tag="ACZPD3_04860"
     CDS             complement(2921..4075)
                     /locus_tag="ACZPD3_04860"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007887240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR1415367.1"
                     /translation="MPLIPLRPLLETVDKYHFAQGAFNVNAVAQAKAAIEVHEMFRSA
                     AILQGADLANGFMGGRTDFQNATLEDKKVGAKNIADAVKKYAENSPIPVVLHLDHGKN
                     FDSCKAAIEGGYTSVMIDGSSLPFDENVELTREVVKYAHERGVSVEGELGVLAGVEDH
                     VFSTTSTYTNPLKAIEFFKKTGVDALAISYGTMHGASKGKNVKLRKEIAIAIKECMMH
                     EGIFGVLVSHGSSTVPKYIVDEINALGGNIQNAYGISIDELKAAIPCGIGKINVDTDI
                     RLAVTRNMKEFFANHPEKRESQSIGEVYRLLEAKKEQFDPRAFLTPIMDTVMYGIIPD
                     EDVAALMAVVEKGVKEAIGTLIVEFGSYGKAPLVEMASLDEMAERYRKGL"
     gene            complement(4088..>4755)
                     /locus_tag="ACZPD3_04865"
     CDS             complement(4088..>4755)
                     /locus_tag="ACZPD3_04865"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046349 - amino sugar biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR1415368.1"
                     /translation="GGNIRYITVSPEVKGVPESIKGMIDLGMKVAIGHSGADYETAWK
                     CINEGAVSATHTFNAMKLLHQHFPAIMGAVLESDIYCEAICDGRHLHPGTVRFLLKMK
                     GLDKVIAITDSIMAAGLPDGEYMLGVNPVIVKDGDAKLKYGDSRAGSTLTTGCALKNL
                     IKFTGRPLEEILPLLTCNPAKMLDVYDRIGSLDTGKDADVVILDKEYSVVDTFVKGRK
                     IEL"
BASE COUNT         1367 a         1140 c          792 g         1456 t
ORIGIN      
        1 gacatcatag agaaaccatg gaatggatgg aatgcatttg tataacgata tttacggata
       61 tattcttcat ttgtagcaaa atactcacca agacggttca tatccggcag agtattcata
      121 tgatcatgct ggaacagatc atataattct cttaaatatg gccagcgctc atcatggaaa
      181 tatccattac atatagatga acagatgatt acacagttat cactcataac tctcttgaat
      241 cgaagcacct gtgcacttaa agcctgcatc atcatgatcg ggttggttcc cattccatca
      301 ccataatgga aattctgtgg cataccaaat acgagtacgt catatttctt ttctgcccaa
      361 tgtacataag ttctcttgtc agcaagtttc cagctgattg gcatcatttc cttggcataa
      421 ccagaataga ttgcgatctg tctggactgt gtatccagta ctgcatcaca gcagaagaaa
      481 ggatgtccca ttttttcttc catatgcata ctgatctcat caaacttgtt acgcatcaga
      541 cttccaccat taaccggtgt aaaatcatct ctgtgcataa cagacggtac atgatgactt
      601 gcgatacatc tccagttagt gatacctgtt gcactgtgtt tgtatccacc tgaatatccg
      661 ccataaggat ttccctgtac atgaccgata agaattggaa tatcacattc aaatacatat
      721 ttattcatgt acaccggatc ccctctgtga gtggttccca gatataccat atgctcctga
      781 tcttcgctgt catgactgat gatctgccct gtatgccaga attcattgaa taattcttct
      841 ccaaagatag cttttgcatc tgcttctgta cttcttggat gaagtccatt ggaaataata
      901 aaaagaatat ctttcttttc aatacctgca gcatataatt cttttaaaat atatttaata
      961 cttaattttc tgtggctggt agcctgctct ccgcctttta cacggtccgg aacaacgata
     1021 gtaactgttt ttccaggtcc tgccagttct gttaaagtag gcattccaat aggatgagcc
     1081 aggctttcca gataagcagc ctcaagctga tcttccggaa tgtaagccgg atcttttaca
     1141 gtctctcctg gaataaatac atcagtattg tccggcagct cagccgccat ggttccctgt
     1201 ccatactcaa aattaagttt catagtaaat ctcccttcat tatatatgct tatttattat
     1261 tttctctacc catatagaga cggataattt caaggtactc ttccggatag tatccaatct
     1321 caccctggaa tcttgtcagt gcgatcagac caccatcaat ctcatcaatg gaagcaagca
     1381 tttctgcgtt atcctgtttc agaccaccgc catatacaat gtccatacca tttgtctttt
     1441 cttttacaaa ttttgcaatt ttggtaatgt attctttacc tgccggtgtt tttcccggtc
     1501 ctatggacca gattggttca tatgcaatca caacttttga agtatctaca tctttgagac
     1561 caatttcaag ctgttttcca agaacttcct gccactgctc ctgttcctca ctcttttcac
     1621 caatgcagta aagaactttc attcctcttt caatcgcaca tttgatttcc tgattcagaa
     1681 gacgatttac agcatctgta tctgtaacac ctgcttctgc gagaattcct gctttgtcat
     1741 ttctttcttc gcaatgtccg ataatagtag tctcacatcc cgcagcaacc atagctgaag
     1801 ccggtctatt tgtagtaaat gcaccaaaat ttccaccaac tgcagtatct gctctgaata
     1861 cactctggca tccaatctga acagggcttc cttctgtttt tgcagccact gcattcagaa
     1921 gatgcacctc tggaaaatac ataccaaatt ctacttcagt aggatcatat gtttttaatt
     1981 cctcctgtgt attttttacg atacaggacg cccagtcagc aactggagcc agacggttta
     2041 cacctccgaa ttccactggg acatcaaatc tttttaaatt cagataaata tgtttcataa
     2101 gattcttacc tttctcttta acaatattaa caagcaaata tctgccatcc gctttcgctg
     2161 tctgctcata ctctccataa aaactcacga caaaaggaga tagcttccag cacatatcat
     2221 aaaaatatat actgtttttt tgattccggc catagtaagt cgtcgaaaca aaaacattcc
     2281 gcctgctgtc ccaaccgctg ttcctgccag tgctgctcca actaaaggca gcatagatcc
     2341 cgatacatga ccctgaaata catgaatcag aaaaatactg atcgtattga ttgtaaaaaa
     2401 catctgaagt gtagcattat atttttcttt atcatctgtt acagaaagaa aatatgctac
     2461 catgggcgga cctccgatgt taaaaagtcc tccacaaaaa ccactgatcg caccggcaac
     2521 cagaccggaa acaaggctgg ccttcagatg aactttactg ctgaagaaga taaaatatac
     2581 agccagaatc agaagtactg ttcccatgat tttcgacaga acatcttcat ccagagccat
     2641 cagcgtgttc acacccagaa aactggttac tgtagccatg atcacaggcg gcagaaggag
     2701 tttccagtca ataaacttcc acagccttac agcaatgtaa attaccataa agaaagctgc
     2761 aatggcttcc agtatggagg ctgtataaaa aggcatgaac atgggccata tagccataca
     2821 gatgatcgca taaccgaatc cactgctgct ctgtacaaaa cttcccgcaa aggccaccag
     2881 tgcagtcaat atatacattt catttacata ccctttttga tcaaagtccc ttacgatagc
     2941 gttctgccat ttcatccagg cttgccatct ctaccagagg agcctttcca taagagccaa
     3001 attctacgat cagagtacca atcgcttctt ttaccccctt ttcaactaca gccataagag
     3061 cagcaacatc ctcatccgga ataataccgt acataacagt gtccatgata ggtgtcagga
     3121 atgcacgtgg atcaaactgc tctttctttg cttccagaag acgatatacc tcgccaatag
     3181 actggctttc tctcttttca ggatgatttg caaagaattc tttcatattt cttgtaacag
     3241 caagacggat atctgtatct acattgatct tgccgattcc gcatggaatt gcagctttca
     3301 gttcgtcaat agaaattccg taagcattct ggatatttcc acccagtgca ttgatctcat
     3361 ctacaatata ttttggtaca gttgaagatc catgagatac cagtaccccg aaaatgcctt
     3421 catgcatcat acattccttg atggcaatag cgatctcttt tcgtaatttg acattttttc
     3481 ctttggatgc accatgcatg gttccataag aaattgcaag tgcatcaaca cctgttttct
     3541 taaagaactc gatagctttc agaggattgg tataagtaga agtagttgaa aatacatgat
     3601 cttccacgcc ggccagaaca ccgagttcac cttctacgga aacacctctc tcatgtgcat
     3661 atttaacaac ttcacgggtc agttccacgt tttcatcaaa tggaagactg gatccatcaa
     3721 tcataacaga agtataacca ccttcaatag ccgctttgca ggaatcaaaa tttttaccat
     3781 gatccagatg aagaacaacc ggaatcggag aattttctgc atatttcttt acagcatctg
     3841 caatattttt tgcaccgact tttttatctt ccaaagtagc attctggaaa tctgttcttc
     3901 cgcccataaa tccatttgca aggtctgcac cctgaagaat cgctgcggaa cggaacatct
     3961 catgaacttc aatcgctgct tttgcctgag ctactgcatt tacattaaaa gcgccctgag
     4021 caaagtgata tttatctaca gtttccagta atggtcttaa tggtattaat ggcataatct
     4081 tttctcctta taattctatt tttctacctt ttacaaatgt atcaacgacg gaatactctt
     4141 tatccagaat aaccacatcc gcatcttttc cggtatcaag acttccaatc ctgtcatata
     4201 catccagcat cttagccgga ttgcatgtaa gaagcggtaa tatctcttca agaggtcttc
     4261 ctgtaaattt aatcagattc ttaagtgcac atcctgtcgt aagtgtactt cctgctctgg
     4321 aatctccata tttcagtttt gcgtctccat cttttacaat aaccggattg actcccagca
     4381 tgtattctcc atccggaagt ccagctgcca taatagaatc tgttatggcg attactttat
     4441 ccaggccttt catcttcaga aggaaacgaa cggttcccgg atgcagatgg cgtccatcgc
     4501 agatggcttc acaatagata tcagattcca gaactgctcc cataatagcc ggaaaatgct
     4561 gatgaagaag tttcatggca ttaaatgtat gcgtagctga tacggcaccc tcattgatac
     4621 atttccatgc agtctcataa tccgcaccag aatgaccgat cgcaactttc ataccaagat
     4681 caatcattcc tttaattgat tccggcactc cttttacttc tggtgaaaca gtaatataac
     4741 ggatatttcc acctg
//
LOCUS       DBMDXG010000131         4737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4364_length_4737_cov_2.140854, whole genome shotgun sequence.
ACCESSION   DBMDXG010000131 DBMDXG010000000
VERSION     DBMDXG010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_4737_cov_2.140854"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            89..520
                     /gene="rpiB"
                     /locus_tag="ACZPD3_04870"
     CDS             89..520
                     /gene="rpiB"
                     /locus_tag="ACZPD3_04870"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR1415369.1"
                     /translation="MIALGCDHGGYELKQEIKKYLDEKGIEYKDYGCDSLDSVDYPIY
                     AKKVAHAILDGECEKGILICGTGIGISITANKIKGIRAAVCTDCFTAEATRLHNDANI
                     LALGGRVVGPGLALKIVDTFLNTPFSNDERHIRRINQIETE"
     gene            745..1236
                     /locus_tag="ACZPD3_04875"
     CDS             745..1236
                     /locus_tag="ACZPD3_04875"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR1415370.1"
                     /translation="MDVNHKRTDYLSWDEYFMGVAMMSGMRSKDPNSQVGACIVSEDN
                     KILSMGYNGFPKGCSDDEFPWAREGDSLHTKYFYVTHSELNAILNYRGGSLEGAKLYV
                     SLFPCNECAKAIIQAGIKTIVYDCDKYADTPAVLASKRMLDAAGVRYYKYNRTGRKIT
                     IEV"
     gene            1792..3231
                     /gene="proS"
                     /locus_tag="ACZPD3_04880"
     CDS             1792..3231
                     /gene="proS"
                     /locus_tag="ACZPD3_04880"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR1415371.1"
                     /translation="MANNKKLVEAITSMEDDFAQWYTDVVKKAELCGYTSVKGCMAIK
                     PAGYAIWENIQHELDRRFKETGVQNVYMPIFIPESLLQKEKDHVEGFAPEVAWVTHGG
                     LDPLQERLCVRPTSETLFCDFYAKEIQSHRDLPKVYNQWCSVVRWEKTTRPFLRSREF
                     LWQEGHTAHATAEEAEERTIQMLNLYADFCEEVLAIPVIKGQKTDKEKFSGAEATYTI
                     ESLMHDGKALQSGTSHNFGDGFARAFGIQYTDKENKLQYVHQTSWGMTTRMIGAIIMV
                     HGDNSGLVLPPRIAPVQAVIIPIQQRKEGVLEKADEMFAALKAAGVRVKVDDTDKSPG
                     FKFAEQEMRGIPIRIECGPKDIENGQAVICRRDTREKYTVTFDELVEKVQEILETIQK
                     DMLERARKHRDSHTYVATNYEEFKDTIVNKPGFVKAMWCGDRACEDKIKEDVQATSRC
                     MPFEQEHLSDVCVCCGKPAKKMVYWGRAY"
     gene            3323..4648
                     /locus_tag="ACZPD3_04885"
     CDS             3323..4648
                     /locus_tag="ACZPD3_04885"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR1415372.1"
                     /translation="MILEIPKNAETILHILEKAGYEAYVVGGCVRDSILGRSPDDWDI
                     TTSAKPEQVKALFHRTVDTGLQHGTVTVLMEKEGYEVTTYRVDGEYEDGRHPKEVTFT
                     ASLKEDLKRRDFTINAMAYNPSSGLVDLFGGLEDIERKIIRCVGDSLERFTEDALRIM
                     RAVRFSAQLGFAIEEETRKALKVLAPNLKHVSAERIQVELVKLLMSPHPDYLRVAYEA
                     GITAEFLPEFDACMTTSQNTPHHCYTVGEHILHSLCHVRANKVLRITMLLHDIGKPVV
                     RKTDENGRDHFKMHGIAGEKMAAQILRRLKFDNDTIRKVTRLVKWHDDRPEGMTKAVR
                     RAVNRIGEDLFPYYLEVQQADMLAQSDYRRTEKQERLDKVKEAYETIINEHQCVSLKT
                     LAVTGKDLIEAGYKPGREIGETLNRLLEVVLADPQKNQKEILLGLLDEK"
BASE COUNT         1477 a          844 c         1244 g         1172 t
ORIGIN      
        1 tatcagcagt aatggccaga tggataatga agattcactt aaatagtctg ctgaagaggt
       61 aaagaaatta atttcaggag gaagattaat gatagcttta ggatgtgatc atggtggata
      121 tgaactgaaa caggagatca agaagtatct ggatgagaaa ggaattgaat ataaagatta
      181 tggatgtgac agtctggatt ccgtagatta tccgatatat gcaaagaagg ttgcacatgc
      241 aattcttgac ggcgaatgtg agaagggaat cctgatctgc ggaacaggta tcggaatttc
      301 cattacagca aataagatta agggaatcag agctgcagtc tgcacagatt gttttacagc
      361 agaggctaca agacttcaca atgatgctaa tatccttgca cttggcggaa gagtggtagg
      421 acctgggctg gcattgaaga ttgtggatac atttttgaat acaccgtttt ccaacgatga
      481 gagacatatc agaagaatta atcagataga gactgaataa gccataaaat aagaaaactg
      541 ataaaacagc cggagcctgc atgattatag taattctctc acatgtatga tgaatgaggg
      601 ttgccgtaga gtaaagtgtt aagttctggc tgtgtcagtt ataagcagat aatggttact
      661 gttcgttccg ttcacagtaa cagacaatgt gctgatttta tgaagcggat gaagggtgaa
      721 gtatgtgaag gggaggtgtg gaaaatggat gtaaatcata aacgcacaga ttatttatca
      781 tgggatgaat attttatggg agttgccatg atgtcaggga tgcgttccaa ggatccgaat
      841 tctcaggtag gtgcctgtat tgtgagtgag gataataaaa ttctgtccat gggttataat
      901 ggttttccaa agggctgttc agatgatgaa tttccatggg caagagaggg tgacagtctt
      961 catacgaaat atttttatgt gacccatagt gaattgaatg cgattcttaa ttacagggga
     1021 ggaagtctgg aaggtgcaaa gctttatgtt tccctttttc catgtaatga atgtgccaag
     1081 gcaattattc aggcaggtat taagacgatt gtctatgact gcgataaata tgcagacacg
     1141 ccggcagtgc tcgcttccaa aagaatgctg gatgcagccg gggtccgtta ttataaatat
     1201 aaccggacag gacgtaagat tacaatagaa gtgtgatttt ggcatattca tgccaaaaca
     1261 tctcgcagga tagtggtgtg tgaacagtaa cgattttact cttgacacat caatttattt
     1321 caaataaaat ataaccaaat gcgaaatata tacagcgatg aagagaatag tacataagga
     1381 gcaatcccag agaagagtcc cggagaacag ccggttggct gtgaaacagt ggtggaagga
     1441 ttctgtgcgt gaattatgga accggcctcg gagctccgta caattgaagt acggcgtttt
     1501 tcctgcgtta tcggaagaag agcgagcaat gtgcaggcat ttgggcacag tgctaattag
     1561 ggtggtaccg ccgaagcctt tcggtccctt gtgagggatg ctgggcttct ttttttcgtt
     1621 ttttataata tgacagattt tgccggatta taaaaaagca gatttttgta ctgtgaagca
     1681 gatagggaaa tttatttata gaagaaaaat aagaagaata tgcattccgc cgcgactata
     1741 tcctgatgca ctgtcaggat caacacaatc acataaaaag gagaagtaat catggcaaac
     1801 aacaagaaac tggtagaagc catcacatcc atggaagacg atttcgccca gtggtatacc
     1861 gacgttgtaa agaaagctga actctgcggt tataccagcg ttaaaggctg catggcaatt
     1921 aaacctgcag gatatgcgat ctgggaaaat atccagcacg aactggacag aagatttaaa
     1981 gagacaggcg tacagaacgt gtatatgcca atctttattc cggaatctct tcttcagaaa
     2041 gagaaagatc atgtagaagg atttgcacct gaggttgcat gggtaactca tggtggtctg
     2101 gatccgcttc aggagcgtct gtgtgtacgt cctacatcag agacattatt ctgtgatttc
     2161 tatgcaaaag aaatccagtc tcacagagac ctgccgaagg tatacaatca gtggtgctct
     2221 gtagtgcgtt gggaaaagac aacacgtccg ttccttcgtt cacgtgagtt cttatggcag
     2281 gaaggccata cagctcatgc tacagccgag gaagcagaag agcgtactat ccagatgctg
     2341 aacctgtatg cagatttctg tgaggaagta cttgcaatcc cggttatcaa gggacagaaa
     2401 acagacaagg agaaattctc cggtgcagaa gctacttata caattgaatc cctgatgcat
     2461 gatggaaaag ctcttcagtc cggtacaagc cataactttg gcgatggatt tgcaagagca
     2521 ttcggcatcc agtatacaga taaagaaaat aaacttcagt atgtacatca gacatcctgg
     2581 ggcatgacaa cacgtatgat cggtgcaatc atcatggtac atggtgataa cagcggtctg
     2641 gtactgcctc cgagaatcgc tcctgtacag gcagtgatca tcccgatcca gcagagaaaa
     2701 gagggcgttc ttgagaaagc agatgagatg tttgcagcat taaaggcagc aggagtccgc
     2761 gtaaaagtag acgacacaga caagagcccg ggattcaaat tcgcagagca ggaaatgcgt
     2821 ggtatcccga tccgtatcga gtgcggcccg aaggatatcg agaatggtca ggctgtcatc
     2881 tgccgtcgtg atacaagaga gaaatatact gttacatttg atgagcttgt ggagaaagta
     2941 caggaaatcc tggaaacaat ccagaaagat atgctggagc gtgcacgtaa gcacagagat
     3001 tcccatactt atgttgctac aaactatgaa gaattcaaag atactattgt taacaaacca
     3061 ggcttcgtaa aagctatgtg gtgcggtgac cgcgcatgtg aggacaagat caaagaagat
     3121 gttcaggcta catccagatg tatgccgttt gagcaggaac acttaagtga tgtctgcgta
     3181 tgctgtggaa aacctgccaa gaagatggtt tactggggaa gagcatacta atcaataaga
     3241 aaataaagaa aaccggcagc agagatatct gagtgccggt tttcggggtt aagaataaat
     3301 agaagaagat aaggatggtc atatgattct ggaaataccg aaaaacgcgg aaacaatttt
     3361 gcatatactg gaaaaagcag gctatgaagc ctatgtggta ggcggctgtg tgcgtgattc
     3421 cattctggga cggagcccgg atgactggga catcaccaca tccgcaaagc cggagcaggt
     3481 caaggctttg tttcacagaa cagtggatac aggtctgcag catggaacag tgactgttct
     3541 tatggaaaaa gaaggatatg aggtcacgac ttatcgtgta gatggtgagt acgaggacgg
     3601 cagacatccc aaagaggtca cctttacagc aagccttaag gaagacctga aacgccggga
     3661 ttttaccatc aatgcaatgg catacaatcc ttcttcagga ctggtggacc ttttcggcgg
     3721 actggaggat attgagagaa aaatcatccg ctgtgtggga gattcgctgg agcgttttac
     3781 agaggatgca ctgcggatca tgcgtgcagt ccgtttcagt gcgcagttgg gctttgccat
     3841 tgaagaagag actcgtaagg cactgaaggt tctggcaccg aatctgaagc atgtcagtgc
     3901 agagaggatt caggtagagc ttgtgaagct tctgatgtct cctcatccgg attatctcag
     3961 ggtggcttat gaagccggga tcacagctga atttcttccg gagtttgatg catgtatgac
     4021 aacatctcag aatacgccgc accactgtta cacagtagga gagcatatcc tgcatagtct
     4081 ctgtcatgta cgtgcaaata aagttctcag gattaccatg ctgcttcatg acattggaaa
     4141 gccggttgtc cggaaaacag atgagaatgg aagagatcat ttcaagatgc atgggattgc
     4201 gggagaaaag atggctgcac agatcctgcg caggctgaaa ttcgataatg atacgatccg
     4261 caaggtaacc cgtctggtaa aatggcatga tgacagaccg gagggaatga caaaggcagt
     4321 gcgcagggca gtgaaccgta tcggtgagga tctcttccca tattatctgg aagtgcagca
     4381 ggcagatatg ctggcacaga gtgattacag acgcacagag aagcaggaac gtctggataa
     4441 ggtgaaagag gcttatgaga cgatcattaa tgaacatcag tgtgtatctt taaaaactct
     4501 ggcagtgaca gggaaagatc tgattgaagc cggttataag ccggggcgtg aaataggaga
     4561 aacgctgaac cgtcttctgg aagtagtgct tgccgatcca cagaagaatc agaaagagat
     4621 attattgggt ctgcttgatg agaaatagtg cagttttatg gaatggtcag tatatcagaa
     4681 tattggttac tgtgaacgga gtgaacagta acgaatatag taaataacag tggctct
//
LOCUS       DBMDXG010000132         4734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4367_length_4734_cov_2.272394, whole genome shotgun sequence.
ACCESSION   DBMDXG010000132 DBMDXG010000000
VERSION     DBMDXG010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4367_length_4734_cov_2.272394"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="ACZPD3_04890"
     CDS             <1..581
                     /locus_tag="ACZPD3_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1415373.1"
                     /translation="EAVQALKAGQIDCVIIDSQPAQKFVENNDDLKILDEPFEEEEYA
                     ICLKKGNDELLDKINGALKELKEDGTIDSIMDNYIGEDAGKTPYESPEDVDRSNGTLV
                     MATNAEFEPYEYHEGDDIVGIDADIAQAICDKLGYELKIEDMEFDSILPAVQSGKADF
                     GAAGMTVTEDRKSSVDFTDTYADASQVIIVKK"
     gene            664..1359
                     /locus_tag="ACZPD3_04895"
     CDS             664..1359
                     /locus_tag="ACZPD3_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1415374.1"
                     /translation="MILASVKEDFYLNFIKDDRYLWLLDGLKTTLIITVFAVIVGLII
                     GFLVAIIRSAHDKSGSFKILNAICRVYLTVIRGTPTMIQLLIMNFVIFGAVSINKIIV
                     GGLAFGINSGAYVAEIVRSGIMSIDQGQTEAGRSLGLNFSQTMRLIIIPQAFKNVLPA
                     LVNEFIVLIKETSIIGYIGMMDLTKGAMLIQSRTYNAFWPLMAAAAIYLVIVGILTWG
                     MNKLERRLRTSER"
     gene            1349..2089
                     /locus_tag="ACZPD3_04900"
     CDS             1349..2089
                     /locus_tag="ACZPD3_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1415375.1"
                     /translation="MSDKNVLIQVKDLKKAFGTNQVLDGITTDICQGEVVAVIGPSGS
                     GKSTFLRSLNLLEVPTGGQILFEGTDITDPKVDINRHRQKIGMVFQQFNLFPHKTVKQ
                     NIMLAPVELKLMTKEEASKKADELLARVGLPDKANAYPDMLSGGQKQRIAIARSLAMN
                     PDVMLFDEPTSALDPEMVGEVLELMKELAQSGMTMVVVTHEMGFAREVATRVLFIDDG
                     KIQEENTPKEFFANPKNPRLKDFLSKVL"
     gene            2091..3041
                     /locus_tag="ACZPD3_04905"
     CDS             2091..3041
                     /locus_tag="ACZPD3_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HIR1415376.1"
                     /translation="MLRKFNSFIEKWMALVTPTCLLLGVCFPDIAKCGLPYVPAVFAF
                     MTFAGALKSRFKDVANVFRHPGSLLIIMLLVHVVIPTASCGAGHLFFGNNMELITGMV
                     LEFAVPTAVVSLMWVTIYDGNSPLSLSLVVLDTVLAPFLIPATLKILLGSAVTIDPAR
                     MMRELIFMVALPAVVAMVLNQITDGKVMETWPGKLAPFSKLALIFVVTSNSSKVAPYI
                     RDMNMQRVKVALAILVLAASGYALGWFVAYIFRKDRSTAVSMMYGTGMRNISAGAVIA
                     AAYFPGEVMFPVMIGTLFQQVLAALFGKLLAQKKEKQEFC"
     gene            complement(3120..3545)
                     /locus_tag="ACZPD3_04910"
     CDS             complement(3120..3545)
                     /locus_tag="ACZPD3_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR1415377.1"
                     /translation="MNILFFLTPKSDVAYIFENETLRQTLEKMEHRKFSCIPLLSLDG
                     KYKGSISEGDLLWGMKTLNIPGLKEAESISIMAIPRRATYKAVHADSDMEDLLDKAIN
                     QNYVPVVDDQGYFIGIITRKEIIKYCYKELKKYIKASDS"
     gene            complement(3654..4661)
                     /gene="asnA"
                     /locus_tag="ACZPD3_04915"
     CDS             complement(3654..4661)
                     /gene="asnA"
                     /locus_tag="ACZPD3_04915"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR1415378.1"
                     /translation="MEQIKIPDGYQSSLNLHDTQIAIKTVKDFFQQTLAQKLNLLRVS
                     APVFVNPSSGLNDNLNGVERPVSFDIKGQPENAEIVHSLAKWKRYALQKYGFAHGEGL
                     YTDMIAIRRDEDLDNIHSVYVDQWDWEKIISKEERNMDTLVSTVRAIYSVLRKTEKYM
                     AVQYDYIEEILPREIAFVSTQELVDMYPDLTPKEREYKIVKEKGAVFLMQVGKTLTNG
                     ERHDGRAPDYDDWELNGDILVYYPVLDIALELSSMGIRVDEDALDRQLTIAGCDDRRE
                     LPFQKAILNKELPYTIGGGIGQSRICMFFLRKAHIGEVHASLWPEEVMKEAAAKGVQL
                     L"
BASE COUNT         1270 a          952 c         1131 g         1381 t
ORIGIN      
        1 gtgaagctgt tcaggcatta aaggcaggac agatcgactg tgtgatcatc gattcacagc
       61 cggctcagaa atttgttgag aataatgatg atctcaagat tctggatgag ccattcgagg
      121 aagaagaata tgcaatctgc ttaaagaaag gcaatgatga acttcttgac aagattaacg
      181 gagctctcaa ggaactgaaa gaggatggaa ctatcgacag catcatggac aattacattg
      241 gtgaggatgc cggtaagact ccatatgaat caccggaaga tgttgaccgt tccaatggaa
      301 ctctggtaat ggctactaac gctgaattcg aaccatatga atatcatgaa ggtgatgata
      361 tcgttggtat cgatgctgat atcgctcagg caatctgtga taagcttgga tatgaactga
      421 aaattgaaga tatggaattc gattccatcc ttccggcagt acagtccggc aaagcagact
      481 tcggtgcagc tggtatgact gtaacagaag accgtaaatc aagcgttgac tttacagata
      541 cctatgcaga tgcaagccag gtaattattg taaagaaata agtattaaaa taacaaataa
      601 cagagctgtt gacgatctgc caacagctct cttttcggaa cagaggtttt tatgaataat
      661 cctatgatat tagcgagtgt aaaagaagat ttttatctga actttattaa agatgaccgc
      721 tatctctggc ttctggatgg tctgaaaaca acccttatta tcacagtatt tgcggtaatt
      781 gtaggtctga tcatcggttt ccttgtagca atcatccgtt ctgcgcacga taagtcaggc
      841 tcatttaaga tcctcaatgc gatctgcaga gtttatctga ctgtaattcg tggaacacca
      901 accatgatcc agctgctgat catgaatttt gtaatttttg gtgctgtaag cattaacaag
      961 atcattgtag gcggacttgc ctttggtatc aactcaggtg cctatgtagc tgagattgtc
     1021 cgttccggta tcatgtccat tgaccaggga cagacagagg caggacgaag ccttggtctg
     1081 aatttcagcc agaccatgcg actgatcatt atcccgcagg catttaagaa tgtacttcct
     1141 gcgttagtaa atgaatttat cgtgctgatc aaagagacat ccatcatcgg ttatatcggc
     1201 atgatggatc tgacaaaagg agctatgctg atccagagcc gtacatacaa tgcgttctgg
     1261 cctctgatgg cagcagcagc gatttacctt gtgatcgttg gaatccttac atgggggatg
     1321 aataaactgg aaaggagatt aagaaccagt gagcgataag aatgtactga ttcaggtaaa
     1381 ggatctgaag aaagcttttg gtacaaatca ggttcttgat ggaataacaa cagatatctg
     1441 tcagggagaa gtagtagccg tcattggtcc ttccggttcc ggtaaatcta ctttcctgag
     1501 atccctgaat cttctggagg ttccgacagg cggacagatc ctctttgaag gaactgatat
     1561 tacagatccg aaggttgata ttaacagaca ccgtcagaag attggaatgg tattccagca
     1621 gtttaatctt tttccgcata aaacggtaaa acagaatatt atgctggcac ctgtagaact
     1681 gaagctgatg acaaaggagg aagcttcaaa gaaagcagac gagcttctgg cacgtgtagg
     1741 gcttcctgac aaagccaatg catatccgga tatgctttcg ggcgggcaga aacagcgtat
     1801 tgccattgca cgttctcttg ccatgaatcc ggatgtgatg ctttttgatg agccaacttc
     1861 cgcacttgac cctgagatgg taggtgaggt tctggaattg atgaaagaac ttgcgcagtc
     1921 aggtatgacc atggttgtag ttactcatga gatgggattt gcaagagaag tggcaacaag
     1981 agttcttttt attgatgacg gtaagataca ggaagagaat actccgaaag aatttttcgc
     2041 gaatccgaag aatcctagac tgaaagactt cctttcaaag gttttataag atgctgagaa
     2101 aatttaacag ttttatagaa aaatggatgg ccctggtgac accgacctgt ctgttactgg
     2161 gggtgtgctt tcctgatatt gccaaatgcg gtctgcctta tgtgccggcc gtatttgcat
     2221 ttatgacatt tgccggggcg ttgaagtcaa gatttaaaga tgtggcaaat gtattccgac
     2281 atccgggctc actgcttatt atcatgcttc tggtccatgt ggtaatcccg acagcatcct
     2341 gcggggcagg ccatcttttc tttggaaata acatggagct gatcaccgga atggtactgg
     2401 agtttgcagt tcccactgca gtggtaagtc tgatgtgggt gacgatttat gatggaaaca
     2461 gtcctctgtc gttgtctctg gtagtgctgg atacggtact ggcacctttt ctgatcccgg
     2521 ctacgctgaa gatcctgctg ggctctgcgg tgaccataga tcctgccagg atgatgcggg
     2581 aactgatatt tatggttgcg cttcctgctg ttgttgcgat ggtattaaat cagatcacag
     2641 atggaaaagt aatggaaaca tggccgggaa agctggcgcc tttttcaaag ctggcactga
     2701 tatttgtggt aacgtccaat tcttccaaag tagcaccata tatccgggat atgaatatgc
     2761 agagggtgaa ggttgcactg gcaatccttg tcctggctgc ttccggatat gcactcggct
     2821 ggtttgtggc gtatatcttc cgaaaggatc gcagtacggc ggtttccatg atgtatggaa
     2881 ccgggatgcg caatatcagc gcaggagcgg tgatcgcagc tgcatatttt ccgggtgaag
     2941 ttatgtttcc ggtaatgatc ggaactctgt ttcagcaggt gttggcagca ttgttcggaa
     3001 agcttctggc acagaaaaag gaaaaacagg aattttgttg aggtctgctg aggcgttata
     3061 aagaatataa aaagaactgc cgcattagca attgaaaaat tgcaatgcgg cagctctttt
     3121 tatgaatctg aagcttttat atatttcttc agttctttgt agcagtattt gatgatctct
     3181 ttacgggtga tgatgccgat aaagtatccc tgatcatcta caaccggaac ataattctgg
     3241 ttgattgcct tgtccagcag atcctccatg tcagaatctg cgtgaactgc cttatatgtt
     3301 gctcttcgcg ggattgccat aatggaaatg ctctctgctt ctttcagtcc gggaatattt
     3361 aaggttttca ttccccagag aagatcaccc tcggaaatac ttcctttata tttcccgtcc
     3421 agactcagaa gcggaatgca ggaaaatttt ctgtgttcca ttttctcaag agtctgacga
     3481 agagtctcat tttcaaatat atatgctaca tcacttttag gtgttaaaaa aaataaaata
     3541 ttcactgttg tattcgcccc gttacatttg tctgataagg cactcagata tacattgtgt
     3601 atttacacat ctgtgtgtct gtgatctctg agtattccag gtttacggct tttttacaga
     3661 agctgtactc cttttgctgc tgcttctttc attacttctt ccggccacag ggaagcatgc
     3721 acttcaccga tgtgtgcttt tctgaggaag aacatacaga tacgggactg gccgatgccg
     3781 ccgccgatag tatatggaag ttccttgttc aggattgcct tctggaaagg aagctcacgt
     3841 ctgtcgtcgc agcctgcgat ggtaagctgt ctgtcaagcg catcctcgtc aacgcgaata
     3901 cccatggaag aaagctccag agcaatgtcg agaacaggat aataaacaag gatatcgccg
     3961 tttaattccc agtcatcgta gtccggtgca cgtccgtcat gacgttctcc gttggttaag
     4021 gttttgccga cctgcatgag gaatacggca cctttttcct tgacaatctt gtattcacgt
     4081 tcctttggag tgagatcagg atacatgtcg accagttcct gagtgctgac aaatgcgatc
     4141 tctctcggaa ggatttcttc gatgtaatcg tactgaacag ccatgtattt ttcggtcttg
     4201 cggagaacgc tgtagatagc acgtactgtg gaaacaagtg tgtccatgtt gcgttcttcc
     4261 ttgctgatga tcttttccca gtcccactga tctacgtata cggagtggat attgtccaga
     4321 tcttcgtctc ttctgatcgc gatcatgtct gtgtaaagtc cttcaccatg agcaaagcca
     4381 tatttctgta atgcatatct cttccatttg gcgagagagt gtacgatctc agcattctcc
     4441 ggctggcctt tgatatcaaa gcttaccgga cgttccacgc cgtttaagtt atcgttcaga
     4501 ccggatgacg gattaacgaa taccggagcg gaaacacgca gcagattcag tttttgagcc
     4561 agtgtctgct gaaagaaatc tttcacagtc ttaatggcaa tctgggtatc gtgaagattt
     4621 aaagatgact gatacccgtc aggaatttta atctgttcca tgagtggatt cctcctgtaa
     4681 tttctgtatt tgcatatttt atggcaaact gataaaaaat attgttgttt attg
//
LOCUS       DBMDXG010000133         4717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4387_length_4717_cov_1.574957, whole genome shotgun sequence.
ACCESSION   DBMDXG010000133 DBMDXG010000000
VERSION     DBMDXG010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4387_length_4717_cov_1.574957"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="ACZPD3_04920"
     CDS             complement(<1..1743)
                     /locus_tag="ACZPD3_04920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415379.1"
                     /translation="MKINSTAAKHIKKYCYIIGLLSFLIAFFACSQMLRYQEKEKKAS
                     GTYMAQSTIRRVKAKLDNYIMISDFLGNYIIDGSDMDENTFSELAEKIPNEEGVVKAF
                     ELAPEGIITEIYPMQGNSEALGLDVLREHERKKDAVLAKETGEYTLGGPYQLKQGGTG
                     ALLFKPVYRTDNFGESSFWGFVLQVIDWDRFMSDINLKSLSEADFSYKIWSYDRSSGD
                     KVILAQSQDDMPENSLTIECEIPNNTWYFDIAPSNGWTPVSQWILSIIASYIFSLLIA
                     MVFYQISSKKHREKQYAAELEKAAELAKSANEAKTRFLFNMSHDIRTPMNAIIGFSGL
                     LEKNLQNEEKAKEYLGKICSSGNLLMTIINQILEIARIESGTTALQLKAEDINTLFHT
                     VNTVFEEDVRKKNLQYSVDLDVYHTFILCDRVKLQEIMLNIISNAIKYTSDGHGVHVK
                     IYEKDSEDPRKARLIFTCEDTGIGMSEEYLPHIFEEFSREHTTTENKVAGTGLGLPIV
                     KSMIELMGGSIRVESTQGAGTKFTVDISFDTASEEDVYRNQISEQPDALKRMEGKRIL
                     LAEDNDLNAEIAIEL"
     gene            complement(1780..2088)
                     /locus_tag="ACZPD3_04925"
     CDS             complement(1780..2088)
                     /locus_tag="ACZPD3_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1415380.1"
                     /translation="MIVTKAWNGREAVEIFENSEPGYFDVILMDLMMPQMGGLEATRR
                     IRKMDREDAESIPIFAMTANAFLDDIAQSKAAGMNEHFSKPLQMEKVIDTIRFYCTAR
                     "
     gene            complement(2098..>4119)
                     /locus_tag="ACZPD3_04930"
     CDS             complement(2098..>4119)
                     /locus_tag="ACZPD3_04930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR1415381.1"
                     /translation="WHEKFLRGETIIIHDLEKICETMPSEYDILKVQDIHSLLVLPLF
                     ANTQLSGFIGLDNPDLVNPGVSISLLSNAGGHLASTLNNLRMFRMLEEKQKTLESNLE
                     ELQKEKHILEALCVDYTSFYLCDLANDTLETIKQSILSNGTEIDEMTEQKSSYTSRIR
                     YYYDHYVIRESAPDFLRKMERHALIEYLQNHKRFAYRYQSVKNHAGHEYFELQVIRLE
                     TGNRDDYKIIMGFRYIDDIVQEDMKKKQQMEETMADLKLNNEIISAISKMYRIIYRMD
                     LIHDTYEEISSQESMHRLTGRSGKTSVQFTKAREKFIAPEFQERMREFLDVSTLAERL
                     KNREEVSTEYRAITGVWYQARFIVKLRNEAGEVTNVLYVTRDINDQKISELESREELR
                     RTAQEAEKANLAKTDFLRRMSHDVRTPINGIQGCVDIADRYPDDLELQQEARTRIRTA
                     SSYLLNLVNDVLDMNKLESGNIQLERKHFNLLELLHNTNEIIRMQANEAGVNYYVEAG
                     EIIHTQLIGSPVHFQQILMNIGSNAVKYNHDGGSVTVTCREISYTDTIAEYELTCTDT
                     GIGMSEEFQKRAFEPFAQEGQSARTKYAGTGLGLAIARKLTELQEGTLFFESIQGKGT
                     KFILHISFEISAEEPEKKSHMYQNCSVEGIQVLLVEDNELNMEIAEFLL"
     assembly_gap    4120..4129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4130..4414)
                     /locus_tag="ACZPD3_04935"
     CDS             complement(<4130..4414)
                     /locus_tag="ACZPD3_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HIR1415382.1"
                     /translation="MKKFKANDNKSEEHEINYRQFNKKLELLNQILSLISDINQASDE
                     KEVKDCIPALLQYTGEYTDADRVYIFELMSEKQDYYSNTFEWCREGIVPQI"
BASE COUNT         1223 a         1135 c          813 g         1536 t
ORIGIN      
        1 cagttctatc gcaatttctg cattcagatc attatcttct gcaagaagaa tacgttttcc
       61 ttccatcctc tttaatgcat ccggctgttc agatatctga ttcctgtata catcctcttc
      121 tgatgctgta tcaaaagaaa tatctacagt gaattttgtc ccggcgccct gcgtactttc
      181 tacccggata cttcctccca tcaactcgat catggatttt acaataggaa gacccagtcc
      241 tgtaccagcg accttgttct ctgtggttgt atgctctctg gaaaattctt caaagatatg
      301 tggaagatat tcctcgctca tgccaatgcc tgtatcttca caggtaaaaa tgagccgggc
      361 tttccgggga tcctctgaat ccttctcata tatctttaca tgaaccccat gcccatctga
      421 cgtatacttt atcgcattgc tgatgatgtt cagcattatc tcctgaagtt tcaccctgtc
      481 acaaaggata aaggtatgat atacatccaa atctaccgaa tactgcaggt tcttcttcct
      541 tacatcttcc tcaaatactg tgtttaccgt atgaaataat gtgtttatat cctctgcttt
      601 cagctgcaga gctgtagtgc cgctttcaat acgggctatc tccaggatct ggttaataat
      661 cgtcatcagc agatttccgg aagaacagat ctttcccaga tattcttttg ccttttcctc
      721 gttttgaagg ttcttttcca gtaatccgga aaaacctatg atcgcattca ttggcgtccg
      781 gatatcatgg ctcatattaa acagaaatcg tgtttttgct tcattggcac ttttcgccag
      841 ttctgccgct ttctcaagct cagcagcata ttgtttttct ctgtgttttt tgctggatat
      901 ctgataaaaa accatagcga tcagcaacga gaatatataa gaagcaatta tggaaagaat
      961 ccattgagaa accggagtcc acccattgga aggcgcaata tcaaaatacc atgtgttatt
     1021 cgggatctca cattctatcg tcaggctgtt ttccggcata tcatcctgac tctgggcaag
     1081 aataacctta tctccactgc ttctgtcata agaccagatc ttataactga aatctgcctc
     1141 acttaaagat ttcagattta tatcactcat gaaacgatcc cagtcaatta cctggagtac
     1201 aaatccccag aaactactct ctccgaaatt atcggttcga taaaccggtt taaaaagtaa
     1261 tgctccggtt cctccctgct ttaactgata gggaccgcca agtgtatatt ccccggtttc
     1321 cttagccaga actgcatctt ttttccgttc gtgttccctc agaacatcca gtccaagagc
     1381 ctctgaattt ccctgcatag gatagatttc ggttatgatt ccttccggtg ccagctcaaa
     1441 cgcctttacc acaccttcct cattaggaat cttttctgcc agttctgaaa aagtgttttc
     1501 atccatatcc gatccatcaa tgatataatt tcccagaaaa tcagaaatca ttatataatt
     1561 gtccagcttt gccttgactc tccttatcgt gctttgtgcc atataagttc ccgatgcttt
     1621 tttttccttt tcctgatatc ggagcatctg actacacgca aaaaatgcga tcaggaaaga
     1681 gagaagtcct ataatatagc agtatttttt aatatgttta gcagctgtac tatttatttt
     1741 cattgaattt tcctcgttat gttttcttcc aactgtttct catcgggctg tgcaataaaa
     1801 tcgtatggta tctatcactt tctccatttg aaggggtttt gaaaaatgtt cgttcattcc
     1861 tgctgcttta ctctgtgcaa tatcatccag aaaggcattg gctgtcatag caaaaatggg
     1921 gatagattcc gcatcctctc gatccatttt tctgattctg cgggtagctt ccagacctcc
     1981 catttgtggc atcatcagat ccatcagaat cacatcaaag tatccgggct ctgaattctc
     2041 aaagatttct acggcttccc gtccattcca tgcctttgtc actatcattt tttcttctta
     2101 cagcagaaac tcagcaattt ccatattcag ctcattgtct tccaccagca gaacctgaat
     2161 cccctctact gaacagttct gatacatatg agatttcttt tccggttcct ctgcagagat
     2221 ttcaaaagaa atatggagaa taaatttggt tccttttccc tgtatgctct caaagaaaag
     2281 agttccttcc tgcagttctg tcagttttct ggcaatggca agacccagac cggtacctgc
     2341 atattttgtt ctggctgact gcccctcctg ggcaaaaggc tcaaatgccc ttttctgaaa
     2401 ttcctcactc atgccgatcc cggtatctgt acaggtcagt tcatattccg caatggtatc
     2461 cgtataggaa atctcacgac aggtaactgt cacagaaccg ccatcatgat tatattttac
     2521 tgcattgctg ccgatattca tcaatatctg ctggaaatgt accggacttc ctatcagctg
     2581 tgtatggatg atttctccag cttctacata ataattaaca ccagcttcat ttgcctgcat
     2641 cctgatgatt tcattcgtat tatgcaaaag ttccagcaga ttaaagtgct ttctctcaag
     2701 ctgtatattg ccggattcca gcttattcat atccagcaca tcattgacaa gatttaaaag
     2761 ataactggaa gcagtcctga ttctggtacg tgcttcctgc tgaagctcca gatcatccgg
     2821 ataacggtcc gcaatatcca cacagccctg gatcccgttg atcggtgtac ggacatcatg
     2881 gctcattctc ctcaggaaat ctgtctttgc cagattggct ttctctgcct cctgagcggt
     2941 tcttctcagt tcttcccggc tctccagctc agatattttc tggtcattaa tatctctcgt
     3001 cacatagaga acattagtca cttcccctgc ttcatttcga agctttacaa taaatcttgc
     3061 ctgataccag actccggtta tggctctgta ttctgtggaa acctcctctc tgttcttcag
     3121 acgctctgcc agagtggaaa catccagaaa ttctctcatt ctttcctgaa attcgggggc
     3181 tatgaatttt tctctggctt ttgtaaactg aacagaagtc tttccacttc tgccggtcag
     3241 acggtgcatg gattcctggc tggaaatctc ctcataggta tcatggatca gatccatccg
     3301 gtaaatgatc cggtacattt tgctgattgc agagataatc tcattattca gtttcaggtc
     3361 agccatagtc tcttccatct gctgcttctt tttcatatcc tcctgcacaa tatcatcaat
     3421 atagcgaaat cccataatga tcttataatc atccctgttg cctgtctcca ggcggatcac
     3481 ctgtaattca aaatactcat gtccggcatg gtttttcact gactgataac gatacgcaaa
     3541 acgcttgtga ttctgcagat actctatgag agcgtgacgt tccatcttcc ggaggaaatc
     3601 cggtgctgat tcccggatta cgtaatgatc ataataatat ctgattctgg aggtataact
     3661 ggatttctgc tccgtcatct catcaatctc tgtaccattg gaaagtatgc tctgtttgat
     3721 cgtttccaga gtatcatttg ccagatcaca taaataaaat gaggtataat ctacgcagag
     3781 agcttccagg atatgcttct ccttctgaag ttcctccaga ttgctctcca gtgttttctg
     3841 tttttcctca agcatcctga acatgcgcag attgttcaat gtactggcaa gatgtccgcc
     3901 tgcattggaa agaagcgata tggaaacacc gggattaacc agatccggat tatccagacc
     3961 gataaaaccg ctcaactgcg tatttgcaaa caaaggtaaa accagcagag aatgaatatc
     4021 ctgaaccttc agaatgtcat attccgaagg catagtttca catattttct ccagatcatg
     4081 aataattata gtttctccac gaagaaattt ctcatgccan nnnnnnnnna atctggggga
     4141 cgatcccctc tctgcaccat tcgaaggtat tgctgtaata atcctgtttt tcagacatca
     4201 gttcaaaaat atatactctg tctgcatctg tatattcgcc ggtgtattga agcaatgcag
     4261 gaatacagtc cttcacttct ttctcatctg atgcctgatt gatgtctgat atcagagaca
     4321 ggatctgatt cagcagttcc agctttttgt tgaattgtct gtagttgatc tcatgttctt
     4381 cactcttatt atcgtttgcc ttaaactttt tcatataatc tcccaacact tatatagaaa
     4441 atactaccgc attaatgcct gaaaacaaaa aaagaacagc cgatacatac gttcgaactg
     4501 ttctcctact tctgaagtcc atacagcatt gacattactg tttcctgctc atttgtactc
     4561 ataccgttcc gtatccataa aacttccctt taagccatta gaggaagtat atttgtaaat
     4621 atcatttaaa gatactttcg tttctgcaaa agtcattttc gcttatatca cttttataat
     4681 atgacagaca gctacgtttg tcacacgaaa gtttaac
//
LOCUS       DBMDXG010000134         4710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4398_length_4710_cov_1.975266, whole genome shotgun sequence.
ACCESSION   DBMDXG010000134 DBMDXG010000000
VERSION     DBMDXG010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_4710_cov_1.975266"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            244..498
                     /locus_tag="ACZPD3_04940"
     CDS             244..498
                     /locus_tag="ACZPD3_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HIR1415383.1"
                     /translation="MRLEKVQEALKTKKIHYEYTEENGCGSIDFMFRGLRFHIWEYED
                     RVWGAETNVFEAGRSQDIEGDYEEIISGEILSWPDMLPGM"
     gene            517..1368
                     /locus_tag="ACZPD3_04945"
     CDS             517..1368
                     /locus_tag="ACZPD3_04945"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1415384.1"
                     /translation="MIFTKKYRTGQIVLTACIVAVCMFSNVHGAEAAGPPAPKTLTVT
                     PTEKPKSTAKSKSASTSKSKSTPKSKSALTQKPEATPIPASTPTSEQEAETDKQNPAD
                     QGTLSKPDHPDTISADKLVFIGDSRTEGLRDAVNDDSIWSCLSSMGYDWMVSTGVPQV
                     EDQIEDNTAVIILMGVNDLYHVNDYVSYINSKAAEWGNRGAQTYFVSVGPVQNDPYCS
                     NAEIESFNAAMQANLSGVTYIDVYSHLVSEGFSTVDGTHYPDSVSVDIYNYILDHLEE
                     QRSGIWG"
     gene            1591..3108
                     /locus_tag="ACZPD3_04950"
     CDS             1591..3108
                     /locus_tag="ACZPD3_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR1415385.1"
                     /translation="MRGLYSSKTEIRHKIFTEIARMAYEGQSPEMLEELPYKIVPGEI
                     ATYRDSIFLERAVVGERLRVAMGMSLRKVTEHAPISKGVEASVIEEKYYEPPLINVIK
                     FACNSCPEKRVMITEGCQGCLEHPCVEVCPKKAVHMEGGRSHIDEDACIKCGKCLEAC
                     PYNAIIKQERPCSKACGMNAIGSDEYGRAEIDQDKCVSCGQCLVSCPFSAIVDKGQIF
                     QTVMALKSETPVYAIVAPAIAGQFPGMENNKIRGAFQALGFTDVREVAVGADLCTVEE
                     AKDFLEEVPEKLPFMATSCCPSWSMMAKKLFPEQAKCISMALTPMVLTARLIKQKEPN
                     CKIVFVGPCAAKKLEASRKSIRSYVDFVLTFEEVAGMFDAKGVDWKDIPEGEPLFRAS
                     ADGRGFAVSGGVAEAVVHAVKRIDPDREVKVMNAEGLQNCKKMLQMAKIGKYNGYLLE
                     GMACPGGCVAGAGTIQTVKKAAAALEKMKKEASFTDAYDSKYRARLESLEKFDVE"
     gene            complement(3377..4477)
                     /locus_tag="ACZPD3_04955"
     CDS             complement(3377..4477)
                     /locus_tag="ACZPD3_04955"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1415386.1"
                     /translation="MILYHISVKKLITRCTFSMLVASLILCPAVFSGCSAKTENVKNT
                     DAGSQDPISATAIKLNTAVTVTIYDSQDRELLTECMNLCDKYEKIFSRTADDSELYQL
                     NHRELTPVKGTEDTYQVSASLAELVSKGLDYSVLSEGAFDIAIEPLTSLWDFTAENPK
                     VPKDSLIQAALPKCNYHNISVDTDKNEITLKTDDTAIELGAIAKGYIADRLKDYLVSQ
                     DVKSAIINLGGNVLCIGEKTDNSAFKIGIQKPFADRSETIAVMDIRDKSVVSSGIYER
                     CFEQDGTLYHHLLNPKTGYPYDNGLIAVTIISDQSVDGDALSTTCFALGLEDGMKLAE
                     SLDDVQAFFVTSDYEIHYTRDFQKKIKVTETE"
BASE COUNT         1451 a          859 c         1159 g         1241 t
ORIGIN      
        1 acggagtgaa cagtaacggc aattgtgaag tggcattcag attacatatt atgcagcaga
       61 gtatgatata attcgttttg tataatggcg tcaggattaa gagcatatgt agagctgtgt
      121 agcgaagcgg attgcgaata tctgtttgac ttttgatggg tgtacacgag gacatttttt
      181 tacagtgaaa gttatctgta ttatattaca gaaggtactg tacagataat caggaggtat
      241 tttatgcgtt tggaaaaagt acaggaagcg ttaaaaacaa agaaaattca ttatgaatat
      301 acagaggaaa atggatgcgg aagcatcgac tttatgtttc gcggtctgag attccatata
      361 tgggaatatg aagatcgtgt ctggggagcg gagacaaacg tgttcgaagc aggcagaagc
      421 caggatattg aaggagatta cgaggaaatt atttccggag aaatcctctc atggccggat
      481 atgctgcctg ggatgtaagt attactgcag aagggaatga tatttacgaa aaaatataga
      541 accggacaaa ttgtattaac tgcatgtatt gtagctgtct gtatgttttc aaatgtccat
      601 ggggcagaag cggcaggtcc gccggcaccg aaaactctga cagttacacc gacagaaaaa
      661 ccgaaatcaa cagcaaaatc aaagtctgcg tcaacatcaa agtccaaatc aacaccgaaa
      721 tctaaatcgg cactaacaca aaaacctgaa gcaacaccaa tacctgcatc tactccaaca
      781 tcagagcagg aagctgagac agataaacag aatccggcag atcagggaac tttaagcaag
      841 ccagatcatc cggatactat ctccgcagat aaactggtat ttatcggaga ttccagaaca
      901 gagggattga gggatgcggt aaatgatgac agcatttggt cctgtctttc ttctatggga
      961 tatgactgga tggtatctac aggagtgcct caggtagaag atcagattga ggacaataca
     1021 gcggttatta ttctgatggg tgttaatgac ctgtatcatg ttaatgacta cgttagctat
     1081 attaattcca aagctgccga atggggaaac aggggagcgc agacttattt tgtgtctgta
     1141 ggtccggtgc agaatgatcc ctattgcagt aatgcggaga ttgaaagttt caatgcggca
     1201 atgcaggcaa atctgagcgg ggttacctac atagacgtat attcccatct ggtatctgag
     1261 ggattttcca ccgtagacgg aacccactat ccggacagtg tgtctgtaga tatttataat
     1321 tatattctgg atcatctgga ggaacagagg agtggaatct ggggatgatt ttttagttta
     1381 tgaaattgtg atgctgtatt gagttcgatg gagtttggtg atatgattgc agaggaaaaa
     1441 acgaaataga gcggaaattc gtttttatat aaaagctgaa aacctggtag gaggaaataa
     1501 tgagtggctg ttttgcagca gagccaaaaa tgtaaatgat aattgcccga tgactggaca
     1561 gttatcaaaa tccaaaatca ggaggaaaga atgagaggac tgtattcatc caaaacagaa
     1621 atcagacaca aaatttttac ggaaattgcg aggatggcat atgaaggcca gtctccggag
     1681 atgctggaag agcttcccta caagattgta cccggtgaaa ttgcgactta tcgtgacagt
     1741 atttttctgg aaagagcggt agttggtgag agacttcgcg ttgcaatggg gatgtcactg
     1801 agaaaggtta cagagcatgc gccaatctca aaaggtgtgg aagccagcgt tattgaggaa
     1861 aagtattatg aaccgccgct tatcaatgta atcaagtttg cctgtaattc ctgccctgag
     1921 aaaagggtaa tgatcacaga gggctgtcag ggctgtctgg agcatccctg tgtggaggtc
     1981 tgtccaaaga aagcagtaca tatggaaggc ggcagatcac atattgatga agatgcctgc
     2041 atcaaatgcg gaaagtgtct ggaagcctgt ccgtataatg caatcatcaa acaggaacgt
     2101 ccctgttcaa aggcctgcgg catgaatgcc attggttctg acgaatacgg aagagcggag
     2161 attgatcagg acaaatgtgt atcctgcgga cagtgtctgg tgagctgtcc gttcagcgct
     2221 attgtagaca aaggacagat tttccagact gttatggcat taaaaagtga aacaccagtg
     2281 tatgcaatcg tggcgcctgc cattgcaggt cagttcccag gtatggagaa taataagata
     2341 cggggtgcat ttcaggcact tggctttaca gatgtaagag aggttgcagt cggtgcagat
     2401 ttgtgcacag tggaggaggc aaaggacttt ttggaggaag ttcctgagaa acttccgttt
     2461 atggctacat cctgttgtcc gtcctggtct atgatggcta agaaattatt cccggaacag
     2521 gcgaaatgta tctccatggc actgacacct atggttctta ctgccagact gatcaagcag
     2581 aaggaaccga attgtaagat agttttcgta ggtccctgtg cagccaagaa gctggaagcc
     2641 agccgtaaga gtatccgcag ctatgtggat tttgtgctga cctttgagga agtggcagga
     2701 atgtttgatg ccaaaggcgt ggactggaaa gatattccgg aaggagagcc actgtttcgt
     2761 gccagtgctg acggaagagg tttcgcagtc agcggtggtg tggcagaagc ggttgtccat
     2821 gcggtgaagc gtattgatcc tgatcgggaa gtaaaggtaa tgaatgcaga aggtctgcag
     2881 aactgtaaga agatgctcca gatggccaag attggtaaat ataatggata tctgctggaa
     2941 ggaatggcat gtccgggcgg ctgtgtagca ggtgccggta ctatccagac agttaaaaaa
     3001 gcagcagcgg ctctcgaaaa aatgaagaaa gaggcttcgt ttacagatgc ctatgactcc
     3061 aaatacagag caagactgga aagtctggag aaatttgatg tggagtgaga ttctgactga
     3121 atcagtgaaa tcctatatgg attgcaagtg tttgaatgca atcagtatat agcagactaa
     3181 aaatgaataa tcgtcagaag aactgtcagg ctgagtgata agtttaactg aatcggataa
     3241 gtcccctgcg gaggttgcga aaagcaataa gcaggggact tttgtcatct gtacaaggtt
     3301 gacttaagtc aaaacaccag ttaataattg ttgatttata tttctgtttt ttcaatttaa
     3361 gtcatcagag acagatttat tctgtttccg taactttgat ttttttctgg aaatctctgg
     3421 tatagtgaat ctcataatca gaagttacga aaaatgcctg tacatcgtcc agagactcgg
     3481 ccagtttcat gccatcctcc agaccaagag caaagcaggt cgtgctcagc gcatccccat
     3541 ccacagactg atcggaaata atggtaactg caatcagccc gttatcataa ggatagccgg
     3601 ttttaggatt gagcaggtga tgatacaaag tgccatcctg ttcaaagcat ctctcataaa
     3661 ttcctgagga aaccactgac ttatccctga tgtccataac agcaatagtt tcgcttcggt
     3721 ctgcaaatgg tttctggatc ccgatcttaa aagcagaatt gtctgttttt tcgccaatac
     3781 agagaacatt tccgccaaga ttgatgatcg cactttttac atcctgagaa accagataat
     3841 ccttcaggcg gtctgcgata taacccttgg caatggcacc gagttcgatt gcagtatcgt
     3901 ctgtctttaa tgtaatctca tttttatctg tatcaacaga gatattgtga taattacatt
     3961 tgggcagtgc tgcctggatc agactgtcct tgggcacttt tggattttca gcagtaaaat
     4021 cccacagaga agtcagcggt tcaatagcaa tatcaaatgc accctcagat aatacagaat
     4081 aatcaagtcc cttagaaacc agctccgcca gagaagccga cacctgataa gtatcctctg
     4141 tccccttcac aggtgtaagt tcccgatggt ttaactgata taattcactg tcatctgcgg
     4201 tgcggctgaa tattttctca tacttgtcac aaagattcat gcattctgta agcagctctc
     4261 tgtcctgaga atcatagata gtgaccgtaa ctgcagtatt cagtttgatc gcagtggcag
     4321 agatgggatc ctgggaaccg gcatccgtat ttttgacatt ttccgttttt gccgaacatc
     4381 cggaaaaaac agccggacat aatataagag atgctacgag cattgaaaaa gtacagcgtg
     4441 ttattaattt ttttacagat atatgatata atatcataaa tgtaacctca tagttatttt
     4501 atggaattgc tataagcaat agttcaggga agaaatatct tttgatccca acaccatagt
     4561 atatcagaaa cagcaaactt tgcacaggta caaaggcaac agaaaaaatc caaaaggaga
     4621 taccggatag aaatgaaata tgccggaaac aaagggagga gtaaataatg tccgatgcag
     4681 taaactgcga atattgtgta aactacagtt
//
LOCUS       DBMDXG010000135         4702 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4401_length_4702_cov_1.454901, whole genome shotgun sequence.
ACCESSION   DBMDXG010000135 DBMDXG010000000
VERSION     DBMDXG010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4401_length_4702_cov_1.454901"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="ACZPD3_04960"
     CDS             complement(<1..830)
                     /locus_tag="ACZPD3_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1415387.1"
                     /translation="MSDNLPRTNRWLIALSVILCLILGGIFTRSSAVTSQADAISDAG
                     AFVSEDQDSAEFTSEETLTDSNDNFTDSSSEKLFRRTSSDSSETSPGGPALSTDISPE
                     ASEGSWASSGSNWMFLVDDKPYTGWFTDTDGKQYYMDETGIMQTGWTDIGKKRYYFDM
                     DGILQTGTVIIDKKTYELDTDGSLKGYTPKKKSSKKKSSDKSATSDKSGTSTAKKSVA
                     LTFDDGPSSFTDRLLDCLEENNAKATFFMVGTEIASFPDEVKRMKKLGCELGNHTYDH
                     "
     gene            1012..1665
                     /locus_tag="ACZPD3_04965"
                     /pseudo
     CDS             1012..1665
                     /locus_tag="ACZPD3_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1754..3712
                     /gene="ligA"
                     /locus_tag="ACZPD3_04970"
     CDS             1754..3712
                     /gene="ligA"
                     /locus_tag="ACZPD3_04970"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1415388.1"
                     /translation="METAAIMRMKELVQKLDRAAKAYYQQDTEIISNREYDQMYDELQ
                     ALEKETGTVLANSPTISVGYEAVDQLPKEEHESPMLSLDKTKDREVLREFIGEHKTLL
                     SWKLDGLTIVLTYENGELAKAVTRGNGVTGEVVTNNARVFKNVPLKIPYQGRLVLRGE
                     AIITYSDFERINESIEDVDAKYKNPRNLCSGSVRQLNNEITARRNVRFYAFTLVSADG
                     VDFHNSRARQFEWLKEQGFDVVEYRTVTASTLDEAMEYFSTAITENDFPSDGLVALYD
                     DIVYGDSLGRTAKFPRNAFAFKWADEIRETHLLEIEWSPSRTGLINPVAVFEPVELEG
                     TTVSRASVHNVSILKELQLGIGDTITVYKANMIIPQIAENLTRSSKLEIPDTCPACGH
                     GTQIQKVNDVEALYCTNPDCAAKKIKSFALFASRDAMNIDGLSEATLEKFIARGFIHD
                     FGDIFEIGKHRDEIVEMDGFGEKSFENLMTSLDKAKETTLAKVIYSLGIANIGLANAK
                     VICRHFDDDLEKIRHADKEEISSIDTIGPVIAGSLTDYFSNEDNNRKLEHLMSHLTVQ
                     KEEKTGEQIFRNMNFVITGSVEHFANRAQAKEFIESLGGKVTGSVTSKTNYLINNDTT
                     SNSSKNKKAKELGIPILSEEDFLKMAQQ"
     gene            3738..4670
                     /gene="metA"
                     /locus_tag="ACZPD3_04975"
     CDS             3738..4670
                     /gene="metA"
                     /locus_tag="ACZPD3_04975"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIR1415389.1"
                     /translation="MPIKIQSDLPVKEILEKENIFVMDESRASHQDIRPIQILILNLM
                     PLKEETELQLLRSLSNTPLQVDVTFMAVQSHEAKNTSVSHLNKFYQTFPELKNNKYDG
                     MIITGAPVEQMEFEEVDYWKELVEIMDWTNTHVTSTIYLCWAAQAGLYHHYGLKKRKL
                     DKKMFGLFWHKVMNRKIPLVRGFDDMFLAPHSRHTEVPIEDIHNCKELTVLAESDEAG
                     LFLAMADGGRKIFVMGHPEYDRVTLDGEYKRDVSKNLPIEIPENYYKDNNPENRPLLM
                     WRAHANNLYTNWLNYYVYQSTPFDLYGTPDFSEI"
BASE COUNT         1492 a          974 c         1113 g         1123 t
ORIGIN      
        1 ttatggtcat aagtgtggtt tccaagttca cagcccagtt tcttcatgcg ttttacttcg
       61 tccggaaaac tggcaatctc agtacccacc ataaagaaag ttgcctttgc attattttct
      121 tccagacagt ccagaagacg gtctgtaaaa gagctgggac catcatcaaa agtaagggcg
      181 accgacttct ttgccgtaga tgtaccggac ttgtcagagg tagctgactt atctgaagat
      241 ttcttcttcg aagatttttt ctttggcgta taacctttca gagaaccatc tgtatccagt
      301 tcataagttt tcttatctat tatcacagtc cctgtctgca gaatgccatc catatcaaaa
      361 taataccgtt tttttccgat atctgtccag cctgtctgca taataccggt ctcatccata
      421 tagtactgtt tcccatctgt atcggtgaac catcctgtgt atggcttatc atctaccaga
      481 aacatccagt tacttccgct ggaagcccag ctgccctctg atgcctcagg cgagatatca
      541 gtgcttaatg caggaccgcc cggagaagtc tcagatgaat ctgaagaagt gcgtctaaac
      601 agtttctcgg aagaagaatc cgtaaaatta tcattgctat cagtcaatgt ttcctcagaa
      661 gtaaactccg cagaatcctg atcttctgac acgaaagctc cggcatcgct gatggcatct
      721 gcctgtgaag tcacagcgga agaacgggtg aaaattcctc caagtatcag acacagaata
      781 acagaaagtg cgatcagcca ccggtttgta cgtggtaaat tatctgacat gaaagcctcc
      841 tttttgataa aatcagtgac aaaaaagata tcctgtcatt cgatatcata caataaaatc
      901 tgacaaaata caaggcgatt ccccattcct gctcttttga aaaaaattcc agaaaaattc
      961 catcaaaatt tcaccgttga taagatgtac aaaaaattat ataatagaac aatggacgaa
     1021 aaaatcagaa taaacaaata tttaagcgag gcaggcatat gctccagacg ggaagccgac
     1081 cggatgatcg aagagggaag gattacggta aacggaaaaa aagcagaaag cggtcagaaa
     1141 gtcagcctgg aagatgagat ctgtgcagac aatatacctg tccataaaaa tgaaaaaaaa
     1201 gtactgcttc tttttaacaa acccagaggg atcgtgtgca gtacaaagca gcagtttgat
     1261 gagacgacgg taacagatta tctggattat cctctgcgtg tttatcctgt gggcagactg
     1321 gataaagaat cacagggcct tctccttctg acaaatgaag gagatctggt caataagatc
     1381 atgcgcgccg gaaactatca tgagaaagaa tattttgtga cagtgaataa gccggtggac
     1441 agggaatttg tccgaaggat gagcaaggga gtccctgttc ttgatacggt gaccagacca
     1501 tgcagagtgg tgcagacagg agaatgcagt tttcggatca ttctgacaca gggactgaac
     1561 cggcagatcc gcagaatgtg ccggtatctg ggatatgagg tacagaagct gaaaaggatc
     1621 aggatcatga atctgactct ggacgggatc agagaaggcg aatacagaga gatcacagca
     1681 caggaatggg aagaactgaa ccatttactg gaatcttcca caagtgaaac agtgatcaga
     1741 acaggagaac agaatggaaa cagcagcgat catgcgaatg aaagagctgg tgcaaaagct
     1801 ggacagggca gcaaaggcgt attaccagca ggatacagag atcataagca acagagagta
     1861 cgatcagatg tacgacgagc ttcaggctct ggagaaagag acaggcactg ttctggcaaa
     1921 cagcccaacc ataagcgtag ggtacgaggc agttgaccag cttccaaaag aagaacacga
     1981 atcccccatg ctgtctctgg acaagactaa ggacagagaa gttctaaggg aatttatcgg
     2041 ggaacacaaa acgcttctgt catggaagct ggacggattg acgatcgttc ttacctacga
     2101 gaatggagaa cttgccaaag cagttacacg tggaaacggt gtgacaggag aggttgtcac
     2161 caataatgcc agagtattta agaacgttcc tcttaaaatc ccgtatcagg gaagactggt
     2221 gttgcgtggg gaagcgatca ttacctattc ggactttgaa cggatcaatg aatccataga
     2281 agacgttgat gcgaaataca agaatccgag aaacttatgc agcggttctg tccgccagtt
     2341 aaacaatgag atcacagccc gcaggaatgt gcgtttctac gcatttacgc tggtaagcgc
     2401 tgacggggtt gatttccata attcaagagc ccggcagttt gaatggctga aagaacaggg
     2461 atttgatgta gtggaataca ggacagtcac agcttcaacg ctggatgaag ccatggaata
     2521 tttttcaact gcgatcactg agaatgattt tccgtcggac ggactggttg ctctttatga
     2581 tgatattgta tacggagatt cccttggaag aacagcgaaa tttcccagaa atgcttttgc
     2641 attcaaatgg gcagatgaga tcagagaaac ccatcttctg gagatcgaat ggagcccgtc
     2701 aagaacaggg ctgatcaatc cggtggctgt ttttgaaccg gttgagctgg aaggaaccac
     2761 tgtgagccgc gccagcgtac acaatgtgag cattctaaaa gaattgcagc ttggtatcgg
     2821 ggatacgatc actgtatata aagcaaatat gatcattccg cagattgcag aaaacctcac
     2881 acgcagcagt aaactggaaa ttccggatac ttgccctgca tgtggccatg gaacacagat
     2941 acagaaggta aatgatgtgg aggcacttta ctgtacaaat cctgactgtg ccgcaaagaa
     3001 gatcaaatcc tttgcacttt ttgcaagcag ggatgccatg aacattgacg gactgtcaga
     3061 agcaacactg gagaaattca ttgcaagagg ctttattcat gatttcggag atatttttga
     3121 gattggaaag catcgtgatg agatcgtgga gatggacgga ttcggggaga aatcctttga
     3181 gaacctgatg accagccttg ataaagctaa ggagactact cttgcgaagg tgatctacag
     3241 ccttggaatc gccaatatcg gccttgccaa tgcaaaagtg atctgcaggc attttgatga
     3301 tgatctggag aagatccgtc atgcagataa agaggagatc agctctattg ataccatcgg
     3361 accggtaatt gccggaagtc tgactgatta tttttcaaac gaggataata acagaaaact
     3421 ggagcatctg atgagccatc tgactgttca gaaagaagaa aagacaggtg agcagatttt
     3481 ccggaacatg aatttcgtta tcacaggaag tgtggagcac tttgcaaaca gagcacaggc
     3541 aaaggaattt attgagtctc tgggaggcaa ggtgaccgga tcagtaacat cgaagacgaa
     3601 ctacctgatc aataatgaca caacttccaa ttcttcaaag aataaaaaag ccaaagaact
     3661 gggaattccg atcctttctg aagaagattt cctgaagatg gcacaacaat aaaataataa
     3721 gaacaggaga taagataatg ccaattaaaa tacagagcga tctgcccgta aaagagattc
     3781 tggaaaaaga aaatattttc gtgatggatg agtcacgtgc atcccatcag gatatccgcc
     3841 ccattcagat cctcattctg aacctgatgc ctctgaaaga ggagacagag cttcagcttc
     3901 tgcgttccct ttccaacaca ccgctgcagg tagatgtcac ctttatggca gtacagagcc
     3961 atgaagcgaa gaatacatct gtgagtcatc tgaataaatt ttatcagaca tttccggaac
     4021 tgaaaaataa caaatatgat ggaatgatca tcacaggtgc tcctgtagag cagatggaat
     4081 tcgaagaggt agactactgg aaggaactgg tagagatcat ggactggaca aacacccatg
     4141 ttacctccac catttatctg tgctgggctg cacaggcagg actctaccac cactatggac
     4201 tgaaaaaacg caaacttgat aagaagatgt ttggtctttt ctggcataaa gtgatgaacc
     4261 gtaagattcc gctggtacgc ggattcgacg atatgttcct tgcacctcat tccagacata
     4321 cggaagttcc catcgaggat atccataact gtaaagagct gacagtgctt gctgaatccg
     4381 atgaagcagg gcttttcctt gcaatggcag acggcggccg caagatcttt gtaatgggac
     4441 atccggaata tgatcgtgtg actcttgacg gtgagtacaa acgtgatgtc agcaaaaacc
     4501 tgcccatcga aatcccggag aattattaca aagacaacaa cccggaaaac agaccgctcc
     4561 ttatgtggag agcacatgcg aacaacctgt atacaaactg gctgaattac tatgtctacc
     4621 agtcaactcc gttcgacctg tatggaacac cggatttcag cgagatctaa agggtttgcg
     4681 agaagttcaa tttcactatc tt
//
LOCUS       DBMDXG010000136         4659 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4458_length_4659_cov_2.218517, whole genome shotgun sequence.
ACCESSION   DBMDXG010000136 DBMDXG010000000
VERSION     DBMDXG010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4458_length_4659_cov_2.218517"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="ACZPD3_04980"
     CDS             complement(<1..406)
                     /locus_tag="ACZPD3_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1415390.1"
                     /translation="MITEKQYLEYLIKQYQNLIYSICLKSVGNPFDAEDLTQEVFLSA
                     YKNLSRFDGNYEKAWLSKIAVRKCLDYLKAAGRRSIPTEDTYFSQIPDRQSSPEDEYM
                     KTASNTHVEALCMQLKSPYKEVAYAHFCKELSV"
     gene            900..2051
                     /locus_tag="ACZPD3_04985"
     CDS             900..2051
                     /locus_tag="ACZPD3_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR1415391.1"
                     /translation="MTETRRLYYEDVYKKEFTATVVECREQKKGYAVIVDESAFYPEG
                     GGQPSDVGTLGDAKVTEVHEKDGELLHYTDKALEVGAKVEGKIDWARRFDLMQQHSGE
                     HMVSGIIHEKYGYDNVGFHMGSDVITVDLNGMLDDSQLAEIEREVNERVWEDREVVIT
                     YPDAEELKTIDYRSKKELTGQVRIVTFPGVDVCACCGTHVTHTGEIGMVKLLSVVKFH
                     DGIRMEMICGKRVLDYLNMVNEQNHQISMKLSAKMDRTADAVQRLQDENFCMKGQVAR
                     MEEEMFRAEAKKWEGAGSVLIFKEGLEADSVRKLADAVMNTCEGCCAVFSRNEDGSYK
                     YAMGEIDGDLRQYTKEMNAALNGRGGGKPFFVQGSVQATEDEIRNFFEK"
     gene            2191..3069
                     /locus_tag="ACZPD3_04990"
     CDS             2191..3069
                     /locus_tag="ACZPD3_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1415392.1"
                     /translation="MFGECHAHIIMDGVNYRHAVEVHKNGPDDKIIREHLKAYQERGI
                     VFVRDGGDALGVSARAKELAPEYGIDYRTPIFAIHKEGHYGSIVGKGFATMVEFHKRV
                     LEAKQAGADFIKIMTTGILDFNEHGAITGTPLDGSEVKEMVHIAHEEGMAVMSHTNGD
                     YGVQAAVAAGVDSLEHGNYMNEESLAMLAESDTVWVPTLVTVRNLLGDGRYDDETLKP
                     IIESAEENIRKAFRLGIKTAPGSDAGAYRVLHGKGIRDEVQSFAEILGDQDAAYRWLA
                     EGEAEIKKKFTVTVHW"
     gene            complement(3253..4431)
                     /locus_tag="ACZPD3_04995"
     CDS             complement(3253..4431)
                     /locus_tag="ACZPD3_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415393.1"
                     /translation="MKKLLAACVCAGLSFSFAGCSPETILEQFTSTGDTSVRELTSSS
                     DTTTETETKDRIYMDELSGTLQDFSGSQLILNTDSASYVFNVSNATLECKGGMITGDE
                     ISIIYEGQLSGTDTGSVHVLKVVDEFHKKNKLKKRTAHGQVISLTSNTITIKSKKGKT
                     ATYPITGTKQYYQNGIKAGDWVYLTFKGKFPDTSNDSSVSLNASHLKVLSISDLKDLE
                     IPDPTPTPDPKLTQTPEQIENKEKQLLATVQGVNLNILRILPAGSDTSFNLDMSAIPA
                     YFKGGIAPGSHVNVTYIGDLETTSLEEVRIIAVTGEDPDTINDKHISFTVTGTIIGST
                     ANTITVQTDDGAIDTFRTENAQDLTAGGMEYGDYVCVTFHPSKSKSSNIYTAIKVQDA
                     "
BASE COUNT         1335 a          798 c         1234 g         1292 t
ORIGIN      
        1 gtactgataa ttctttgcag aaatgtgcgt aagcgacttc tttgtaagga cttttcaact
       61 gcatacacaa tgcttctacg tgagtattgg atgcagtttt catatattca tcttcgggag
      121 aggattgcct gtccggaatc tgggaaaagt aggtgtcttc tgttggaata cttcgccgcc
      181 cagctgcttt cagataatcc agacactttc ggacagcgat cttacttaac caggctttct
      241 cataattacc atcaaagcgg gaaaggttct tataggcaga cagaaagact tcctgagtga
      301 gatcttcggc atcaaatgga ttaccgacag atttcaggca gatcgaatat attaagttct
      361 ggtattgctt aatcagatat tccagatatt gcttttctgt gatcattact gtctcctttt
      421 ccctgttcat ttaattataa cgcaggaggt gggggaatgg attcagatat tggaaatttt
      481 gagaaaaaag ggacgcgcag aaaaatagtt tgttttgtgt gcggtttcgg acactaagat
      541 attctaagct gccaaaaatc tgctaaaggc atgagaaaaa tgccacaaac tcgccgcatg
      601 gggctcagac agtgtgtcat ttttctcata acgcagattt ttgccggcta agaatatcta
      661 agtatcctgc aaatgcacac aaaacaaact atttttctgt gctggttgtt ggctgttggt
      721 tagcggattt tttataaaaa tatctctaag tgatggtctt gcgaacctgt ctgatttggt
      781 ctatacttga ttgaaatgaa atgaaatgaa atgaaatgaa atgaaatgaa atacagcatt
      841 tatgtttata tggaagataa tgcggggcaa tatgacagat taacaaggag agacagatca
      901 tgacagaaac aagacgttta tactatgaag atgtatataa aaaagaattc accgcaaccg
      961 tagtagagtg cagagagcaa aagaaaggtt atgcggtcat tgtggatgag agtgcttttt
     1021 atccggaggg cggcggtcag ccaagtgatg tgggaacact gggggatgca aaggtgacag
     1081 aggttcatga gaaagatgga gaattacttc attatactga taaggctctg gaagtgggtg
     1141 cgaaggttga aggaaaaatt gactgggcgc ggcgttttga cctgatgcag cagcattccg
     1201 gtgagcatat ggtcagcggt attatccatg agaagtatgg ttatgataat gtaggttttc
     1261 atatgggaag cgatgtgatc actgtagatt tgaatggtat gctggatgac tcgcagcttg
     1321 cagaaattga gcgggaagta aatgagagag tgtgggagga tagggaagtt gtaattactt
     1381 atcctgatgc tgaggaactg aagactattg attacagaag taagaaagag ctgacaggac
     1441 aggttcgtat tgtgacgttt ccgggagtag acgtatgcgc ctgctgtgga acccatgtga
     1501 cacatacagg tgagattgga atggtgaagc ttctttctgt agtgaaattc catgatggta
     1561 tccgtatgga gatgatctgc ggtaaacgtg ttctggatta tctgaatatg gtgaatgagc
     1621 agaatcatca gatttccatg aagctttctg caaagatgga cagaacagca gatgcagtac
     1681 aacgtcttca ggatgagaat ttctgtatga aaggccaggt tgcccgtatg gaagaagaga
     1741 tgtttagggc agaggctaag aaatgggaag gtgcaggaag tgtcctgatc ttcaaagaag
     1801 gtctggaagc agattctgta cgtaaacttg cggatgcagt gatgaatacc tgtgagggct
     1861 gctgtgcagt attttccaga aatgaagatg gaagctataa gtatgccatg ggcgagattg
     1921 acggggattt acggcagtat acaaaggaaa tgaatgctgc attaaacgga agaggcggcg
     1981 gaaagccatt ctttgtccag gggtctgtgc aggcaacaga agacgaaatc cgaaattttt
     2041 ttgaaaaata aaaaattgta gataaaagat taagtaaaac tgcggagtgg ttattgagca
     2101 tagagttgca tagcgaagcg gatgagttta tccgtctgac tctatgtttt gaattaagaa
     2161 agccgcaggg aagaggaaga gaaacgggaa atgtttggag aatgtcatgc gcatattatt
     2221 atggatggtg tgaattaccg ccatgcagtt gaggtgcata aaaatggacc ggacgataag
     2281 atcataagag agcatctgaa agcataccag gaacggggaa ttgtttttgt acgtgatggt
     2341 ggtgatgcgt tgggtgtttc tgcgagagcg aaagagcttg caccagagta tggaatagat
     2401 tacagaactc cgatatttgc cattcataaa gaaggacatt atggctcgat tgtgggaaaa
     2461 ggttttgcca caatggtgga atttcataag cgtgttctgg aggcgaaaca ggcaggggca
     2521 gatttcatta agattatgac taccgggatt ctggatttta atgaacatgg agccattacc
     2581 ggaacacctc tggatgggtc agaggtaaag gaaatggtac atattgcaca tgaagaaggt
     2641 atggctgtca tgtcccatac caacggagat tacggagtgc aggcagcagt ggctgccgga
     2701 gtggacagtc tggagcatgg aaactatatg aatgaagaaa gtcttgccat gctggcggag
     2761 agtgatacag tctgggttcc gactctggtt acagtaagaa atcttctggg agacggacgt
     2821 tatgatgatg agactctgaa accaatcata gaatctgcag aagagaatat cagaaaagca
     2881 ttccggctgg gaataaaaac agcccccgga agtgatgcgg gggcttacag agttcttcat
     2941 ggaaaaggaa tccgggatga agttcagtcc tttgcagaaa tcctgggaga tcaggatgct
     3001 gcataccgct ggcttgcgga aggtgaagcg gaaatcaaga agaaatttac ggttactgtt
     3061 cactggtgat atcccgcgag gtgttttgtg tgagcgaagg tcacagaaaa cccgagacat
     3121 accgcgcgaa tttatgcgat tagcataaat taagtttgtg aagcaaacga tactccttgg
     3181 gcattgcaca aggaggttca tatttcctgc atcgcgaagc gtgttgctga gaacggagtg
     3241 aacagtaaca atttacgcat cctgtacctt gattgctgta taaatattag aagattttga
     3301 tttggatgga tggaaggtaa cacacacata gtcgccgtat tccatgccgc ctgcagtgag
     3361 atcctgcgcg ttttctgtac ggaaggtatc tatggcaccg tcatctgtct gtacagtgat
     3421 ggtgttggca gtacttccaa tgatcgtgcc ggttactgta aaggaaatat gtttgtcatt
     3481 aatggtatca ggatcctcac ctgtcacagc aataatacgg acttcttcca gggatgttgt
     3541 ttccagatct ccgatataag taacattaac atgagatccc ggggcaattc ctcccttgaa
     3601 gtaagcgggg attgctgaca tatccagatt aaaggaggtg tcacttccgg caggaagaat
     3661 ccggaggata ttcagattta ctccctgtac agttgccagc agctgttttt ctttattttc
     3721 tatctgctcc ggtgtctgtg tgagttttgg atccggagtt ggggtaggat ccggtatttc
     3781 cagatctttc agatcagaaa tactcagtac tttcaggtga ctggcattta aggatacgga
     3841 agaatcattg gaggtatccg ggaatttccc cttgaatgta aggtaaaccc agtcaccggc
     3901 tttgattcca ttctgataat attgttttgt tcctgtgatt ggataggtgg ctgtttttcc
     3961 tttctttgat tttattgtga tggtattgga agtcagggaa atgacctgcc catgcgcagt
     4021 gcgctttttc agcttatttt ttttatgaaa ttcatctaca accttcagaa catgtacact
     4081 gcctgtgtca gtaccggaaa gctgaccttc gtaaatgata ctgatctcat cgccggtgat
     4141 catgccgcct ttacattcca gggtggcatt ggaaacatta aatacatagg aggcactgtc
     4201 cgtgttcaga ataagctggc ttcctgaaaa atcctgcagg gtaccggaaa gttcatccat
     4261 atagatacgg tcttttgttt cggtttcagt tgtggtgtca gatgaagaag taagttcccg
     4321 gacggatgtg tctccggtgg atgtgaattg ttccagtatg gtttcagggc tgcatccggc
     4381 aaaagaaaag ctaaggccgg cacagacaca tgcagcaagc agttttttca taatacagac
     4441 aggacctccg ataaatcatt cgtttgaaca tgattcctgc cgtatgcaat aattatttac
     4501 agggagggat attgctttcc ggcggttcgg ggataaaatc aaaaattttc cggtagcttt
     4561 cccattcgtc attgtctttg gcaggagtgc atatcaggcc ggtacgttcc tgtgaggatg
     4621 tggatgaagc agcacttact tatgcggagg tcaaggctt
//
LOCUS       DBMDXG010000137         4597 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4523_length_4597_cov_1.811165, whole genome shotgun sequence.
ACCESSION   DBMDXG010000137 DBMDXG010000000
VERSION     DBMDXG010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_4597_cov_1.811165"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="ACZPD3_05000"
     CDS             complement(<1..516)
                     /locus_tag="ACZPD3_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415394.1"
                     /translation="MKITTEDIQVGFHARQILKGISIESKDKELVGIIGPNGSGKSTL
                     LKCIYRILKPDAGAVYLDGEELHSMSVKSSARKMAVVAQHNYYNFDFTVREVVLMGRA
                     PHKKALERDNAKDYRIVEEALKTVQMDAFADRTFSTLSGGEQQRVILARALAQQTPAL
                     ILDEPTNHLDIT"
     gene            complement(516..1601)
                     /locus_tag="ACZPD3_05005"
     CDS             complement(516..1601)
                     /locus_tag="ACZPD3_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1415395.1"
                     /translation="MKEQVSQTKKGSVLRTSMYVAVLIGLAAFLIFSILAAITFGNAD
                     LSLKDVYSVIAYKLFHIKSLSAYAEGAVHDVVWLIRLPRVLLALAVGMALSVCGVVMQ
                     AIVQNPLADPYVLGISSGASLGATLAIMLGVGGFLGGNSVGVPAFIGAMVTSFAVIAI
                     ANMGGKATSAKLILAGMAVSAVCSAFSNFVIYITNDKNAATEVMKWTMGSLAGASWSR
                     VGVMLPVTLICVIIFWTQYRNLNLMLLGDDVSITLGTDLHRLRTFYLIVASVMIGFAV
                     YCAGVIGFVGLVIPHVIRILFGTDHRRLLPLSALLGASFLIWCDVACRVILKNSEMPI
                     GVLVSIIGAPCFIYLLVRKSYGFGGSK"
     gene            complement(1616..2740)
                     /locus_tag="ACZPD3_05010"
     CDS             complement(1616..2740)
                     /locus_tag="ACZPD3_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1415396.1"
                     /translation="MKNRYLSTRIMAIMMAAAMAVSAAPAAFAAERADSETIKEDNQP
                     AADADSDTQEDADAQDSADADKTADAEGTKTEYPLTITTYDYDGNEIETTYEKAPEKV
                     LAVYQGSIETMLALGLEDRLVATAGLDNEVPDELKDAFSKTNYLDEFTPSLETVTMLE
                     PDMILSWSSLFSDKNLGNVTDWIDKGCNTYYNTNTRPDGDRTLENEFTDILNLGKIFD
                     VQDKAQAIVDDAKAVIDKTLTATADVEEKPSVMVLEPLGEDITNYGAKSLGGDMVTQL
                     GATLANPDASPAGKEDIIAANPDVIFVVYMPYAGDDPETVKESQLAVIKDDEALQSLD
                     AVKNGRVYPIMLSEMYASATRTQDGIETFAKGLYPDVNLD"
     regulatory      complement(2856..3135)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3211..3861)
                     /locus_tag="ACZPD3_05015"
     CDS             complement(3211..3861)
                     /locus_tag="ACZPD3_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR1415397.1"
                     /translation="MKGLFVKDIELMKQQKQFFILVVVMGVILNLAGSGSVSFATGYF
                     TIVTAIFAITTISYDEFDNGLAFLMTLPVTRKQYVAEKYLLGAGLTAVAWGIATITGV
                     ICKGVAELQGCLSETIIGSLIYIPLALLMLAVSLPLVIHFGAEKGRYIAMVMWAIIFA
                     VVYILIKTMGLSADAVDAWLNGLNRGMVLAGVVLFTVIVYMGSFWIGVRLMEKKEF"
     gene            complement(3858..>4597)
                     /locus_tag="ACZPD3_05020"
     CDS             complement(3858..>4597)
                     /locus_tag="ACZPD3_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1415398.1"
                     /translation="GKSTTFKALLGLIHPDGGEIEVFGKKAEELKPEDKQKLGVVFAD
                     SGFSMYLTAAGVANIMKSIYPDFDREEFLQQCRRFNLPTDKKIKEFSTGMKAKFKVLA
                     ALSHKAELLILDEPTVGLDVIARDEVLNMLREYMEENESSSILISSHISSDLESLCDD
                     FYMIHAGKIILHEDTDVLLSDYAVLKVSEEEYEKLDQQYLIKIRKEAYGYRCLTNQKQ
                     FYMENYPDVVIENGKIDDLVVMMEEQV"
BASE COUNT         1260 a         1149 c          827 g         1361 t
ORIGIN      
        1 tgtgatatcc agatggttag tcggctcatc aaggatcagt gcaggtgtct gctgtgcaag
       61 tgctcttgcc agaataaccc tctgctgctc tccacctgac agcgtagaaa atgtgcgatc
      121 cgcaaatgca tccatctgta ccgttttcag tgcttcttca acaatccggt aatcttttgc
      181 attatcccgt tccaatgctt tcttatgagg tgcacgtccc atcaacacga cttcccgtac
      241 ggtaaaatca aagttgtagt aattgtgctg tgccacaact gccatttttc tggcggagct
      301 ttttacgctc atggaatgga gttcttcgcc gtccagataa acggcacctg catctggctt
      361 caggatacgg tagatgcatt ttaataatgt agattttccg cttccgtttg gtccgatgat
      421 ccctaccagt tccttatcct ttgactctat ggaaattccc ttcagtatct gtctggcgtg
      481 aaatccgacc tgaatatctt ctgtagtgat cttcattact tactacctcc aaatccatag
      541 gatttccgca caagcagata aataaagcac ggagcaccga taatggatac caacacaccg
      601 atcggcatct cagaattctt caggatcaca cgacatgcca catcacacca gatcaagaac
      661 gatgcaccaa gcagtgcact taatggcaat aatcttctgt ggtctgttcc aaaaaggatt
      721 ctgatcacat gagggatcac aagtcctaca aagccgatca cacctgcaca atatactgca
      781 aacccgatca ttacagaagc aacgatcaaa taaaaagttc tcagcctgtg caaatcagtt
      841 cccagtgtga tcgatacatc atctcctaac agcatcagat tcaaattacg atattgcgtc
      901 cagaaaataa ttacacagat caatgtaaca ggaagcatta cacctactct gctccagctt
      961 gcgcctgcaa ggcttcccat cgtccacttc attacttctg ttgctgcatt tttgtcattg
     1021 gtgatataga taacaaaatt ggaaaaagca gaacataccg cactgacggc cattccggca
     1081 aggatcagtt tagcagaagt agcttttccg cccatgtttg caatggcaat tactgcaaaa
     1141 gaagtgacca ttgcaccaat aaatgcagga acaccaactg agtttccacc gagaaatcca
     1201 ccaactccaa gcatgatcgc cagtgttgca ccaagggatg caccggatga aataccaagt
     1261 acatacggat ctgccagtgg gttctggaca atcgcctgca taactacgcc acagacggaa
     1321 agagccattc caactgccag agccagcagg actctgggca gccggatcag ccacacaaca
     1381 tcgtgaactg cgccttctgc ataggcggac aggcttttga tatgaaacaa tttatatgca
     1441 atgacactgt aaacatcttt cagtgacaga tctgcatttc caaaagtgat tgctgcaaga
     1501 atggaaaaaa tcaaaaatgc cgcaagcccg ataagaactg ccacatacat ggatgtcctg
     1561 agcacactgc ctttttttgt ctgtgaaacc tgttctttca ctgttatgtc ctcaatcagt
     1621 caagattcac atccggatat aatccttttg cgaaagtttc aataccatcc tgtgtacgtg
     1681 ttgcacttgc gtacatttca ctgagcatga tcggataaac acgaccgttc tttacagcgt
     1741 caaggctctg aagtgcttcg tcatccttga taacggcaag ctggctttct tttacagttt
     1801 ccggatcatc acctgcatat ggcatgtata caacaaaaat cacatcaggg tttgctgcaa
     1861 tgatatcttc ttttcctgct ggagaagcat ccgggtttgc cagagtcgca cccagttgtg
     1921 ttaccatatc tccaccaagg ctctttgcac catagtttgt gatatcttct ccaagaggtt
     1981 ccagaaccat tacgcttggt ttctcttcta catctgctgt tgctgtaaga gttttatcga
     2041 tcactgcctt tgcatcatct acgattgcct gtgctttgtc ctgtacatca aaaatctttc
     2101 caagatttaa gatgtctgta aattcatttt ccagagttct gtctccgtca gggcgtgtgt
     2161 ttgtgttgta ataagtgttg cagcctttat cgatccagtc tgttacattt ccgagatttt
     2221 tatcagaaaa cagggagctc caggataaga tcatgtctgg ctccaacatc gttacagtct
     2281 caagggatgg ggtaaattca tcaagataat ttgtttttga aaatgcatct ttaagttcat
     2341 ccggaacttc attgtcaaga cctgcagtag ctaccagacg gtcttccagg ccaagtgcaa
     2401 gcatggtttc aatgcttccc tgatatacag caagaacttt ctccggtgcc ttttcatagg
     2461 ttgtttcgat ttcatttcca tcataatcat aggttgtgat ggtcagagga tattctgtct
     2521 ttgtaccttc tgcgtctgct gtcttatctg catctgctga atcctgtgcg tctgcatctt
     2581 cctgtgtatc agagtctgca tctgctgccg gctgattgtc ctctttgatt gtctcagagt
     2641 ctgctctctc tgctgcaaaa gctgccggag ctgcggaaac tgccatagct gctgccatca
     2701 taattgccat gattcttgtg gataaatacc tgtttttcat ttgacttcct ctcttttcgt
     2761 atatgttatt tacctgttcg tgtaaccgaa aagatgggac ttcagaaaaa gtaaaataga
     2821 agtgataaaa ataacggata ctttttacgg aagctccttc tttacactgt caggcaggtt
     2881 tcctgactca cagatcacag aaagtttcca caaaataaaa actgatacaa catccatgct
     2941 tcagcagttc atgaatgttg tatcagaaat atttccagat actctctggc agccggattc
     3001 cgccttctca catatttatg caatgacata ttacaatccc gcttttttct gtatacagtg
     3061 gccgcaaccg ttcaggcatt tcacctgatt ccctctcaca ttatcataca caaaatctgc
     3121 atatgataat gttcacttaa cagcttattc aattacgctg atacaatttt agcacactgc
     3181 ttttataatt acaactatat tcgagacaca tcagaattct ttcttctcca taagccggac
     3241 accaatccaa aaagacccca tatatacaat tactgtaaat aatacaactc cggccaatac
     3301 cattccccgg ttcagtccat tcagccatgc atcgactgcg tctgctgaaa gtcccattgt
     3361 ttttatcaga atataaacta cggcaaatat aatagcccac atgaccattg caatataacg
     3421 tcctttttcc gctccaaaat gtattacaag cgggagcgat accgccagca taagaagtgc
     3481 cagtggaatg tatatcaggc tgccaatgat agtctcactc agacacccct gaagttctgc
     3541 cacacctttg cagattactc ccgttatcgt tgcaattccc cacgctacag ccgtaagccc
     3601 tgctcccagc agatattttt ccgccacata ctgttttctg gtaaccggca aggtcataag
     3661 aaacgcaaga ccattatcaa actcatcata acttatagtc gtaatggcaa aaattgctgt
     3721 gacaatcgta aaatatcctg tagcaaaact gacgctcccg cttcctgcca gattcaggat
     3781 gactcccatt acaacaacca ggataaaaaa ctgtttctgc tgtttcatca gttctatatc
     3841 ctttacaaat aatcccttca tacctgttcc tccatcatca ctaccagatc atcaatcttt
     3901 ccattctcaa ttacaacatc cggataattt tccatataaa attgtttctg gtttgtcaga
     3961 cacctgtatc cataagcttc tttccggatc ttaataaggt attgctgatc cagtttttcg
     4021 tactcttctt ctgaaacttt aagaactgca taatctgaca gcagtacatc tgtatcctca
     4081 tgaaggataa tttttccggc atggatcata taaaaatcgt cacaaagact ctccagatct
     4141 gatgaaatat gcgaactgat cagaatagaa ctgctttcat tttcttccat atattctctc
     4201 agcatgttca gcacctcatc cctggcaatc acatccagac caactgtggg ctcatccaga
     4261 atcagcagtt ctgccttatg gcttaatgct gccagtactt taaactttgc tttcatgcca
     4321 gtggaaaatt ctttgatttt cttgtctgtg ggcagattga atcttctgca ctgctgcaga
     4381 aattcctctc tgtcaaaatc cggatatatg cttttcataa tattggccac gcccgctgct
     4441 gtcagataca tactgaagcc tgaatctgca aaaacaactc ccagtttctg cttatcctcc
     4501 ggcttaagct cttctgcctt tttcccaaag acctctatct ctccgccatc cggatgaatc
     4561 aagccgagca aagctttaaa tgtggtggat ttcccgg
//
LOCUS       DBMDXG010000138         4583 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4543_length_4583_cov_2.189647, whole genome shotgun sequence.
ACCESSION   DBMDXG010000138 DBMDXG010000000
VERSION     DBMDXG010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4543_length_4583_cov_2.189647"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            143..799
                     /locus_tag="ACZPD3_05025"
     CDS             143..799
                     /locus_tag="ACZPD3_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1415399.1"
                     /translation="MVFNRTTETDLTEDQLPQVDIYKYKQVIFVGDSRTEFMENVLTG
                     MGESATKNVKFVCSAGKGLDWFTTTGWAQLYSIVQHDSNSILSKKTAVIFNFGVNDLS
                     KSADYAEYYNWIAPQLKSKGCELYFMSVNPVNRLMLPNAGRADRSEAAVRSFNQYMKA
                     NLSSAYTYIDMYSYLKSTGYSFASDHYGTGTVDDGLHYTTRTYKRIFAKCMDSLRVPA
                     "
     gene            985..2376
                     /gene="asnS"
                     /locus_tag="ACZPD3_05030"
     CDS             985..2376
                     /gene="asnS"
                     /locus_tag="ACZPD3_05030"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR1415400.1"
                     /translation="MKLTTVKEIYKEREKYLDKEVTVGGWVRSVRDSKTFGFIVLHDG
                     SFFETLQVVYHDTMENFAEISKLNVGAAIIVKGTLVATPQAKQPFEIQAAEVTVEGNS
                     APDYPLQKKRHSLEYLRTITHLRPRTNTFQAVFRVRSLCAYAIHRFFQEQGFVYVHTP
                     LITGSDCEGAGEMFQVTTLDMNNVPTDDKGAVDYSQDFFGKETNLTVSGQLNCETFAQ
                     AFRNVYTFGPTFRAENSNTTRHAAEFWMIEPECAFADLNDNMDLAEAMLKYVIRYVLE
                     NAPEEMNFFNSFVDKGLLDRLNHVINSEFGHVTYTEAVELLEKNNDKFDYKVFWGCDL
                     QTEHERYLTEEIFKKPVFVTDYPKEIKAFYMKMNEDNKTVAAMDCLVPGIGEIIGGSQ
                     REDDIEKLEKRMDELGLKKEDYDFYLDLRKYGSTRHSGFGLGFERCVMYLTGMGNIRD
                     VIPFPRTVKNCDL"
     gene            2461..3513
                     /locus_tag="ACZPD3_05035"
     CDS             2461..3513
                     /locus_tag="ACZPD3_05035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1415401.1"
                     /translation="MRFIHLADVHLGAVPDRGCPWSSRREEEIWETFRRVIAGIRENP
                     VDLLFIAGDLFHRQPLLRELKEVNNLFSTIPDTRVYLMAGNHDYIKADSFYRDFQWEK
                     NVTFFKNEQLTCVKDEKLDVYVYGLSYEHQEIENPLYDSIHPAEGEGVHILLAHGGDA
                     KHIPMNIKSIAASGFDYIALGHIHKPQILIRDNAAYAGALEPVDRNDLGDHGYIEGHL
                     ENGRLKTNFVPFACRSYEQILLMLREDSTQASLEDMLKADLANKGRMNIYRIVIQGTR
                     APELLLLPERLKSFGYVTEVLDESKPSYDLEALQKKYSGTLIGDYISYFLEKDRNAVE
                     EKALYYGLQALLETSR"
     gene            3479..>4583
                     /locus_tag="ACZPD3_05040"
     CDS             3479..>4583
                     /locus_tag="ACZPD3_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0061768 - magnesium:sodium antiporter
                     activity [Evidence IEA];
                     GO_process: GO:1903830 - magnesium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC41A family transporter"
                     /protein_id="HIR1415402.1"
                     /translation="MVFRLYWKQAGDRQNMIIRQLNIKNSGEIHDKTLEFSPGINVLY
                     GENESERAIVHTYIKNMFLGDISEAIYDNAVFAAKLKSPTEQDLARELQKFMTGYQGT
                     ADSSMNLGRAMQMLKMSRKGYLVQADRKQKETEQERQKLLTRMDDIISDLNDLNGKLD
                     QINEKEESLRMHPGDENGAVILDERMAQAKAKRNSFAMGMLISATAGIFGLILTAIIA
                     DSVSVSLIVAVIAAALVGFCGKQQLKYAREFQKRIRMKKRWVSRQEKLRCSKENLRQD
                     YDEKETELCNLKEEYREYEENSFLLTSEEREVQALNMAMETIERMSGNIHLQVGRKLQ
                     MRTSQILSEITDGEYQDVQMDAASHMTVTTGNGA"
BASE COUNT         1436 a          817 c         1150 g         1180 t
ORIGIN      
        1 aagttcttat tctcttccgg gcgtaaggga tgcagaaggt tatactttta tggggtggtc
       61 cacaaaaccg atgcagagtg tttctccgca gtatgaggca taagaaaaaa tcacggtaaa
      121 tggaaatgtg gatctctatg cagtggtatt taacagaaca acggaaacag accttacaga
      181 ggatcagctt ccgcaggtag atatctataa atataaacag gtgatttttg taggtgattc
      241 ccgtacggaa tttatggaaa atgttctgac aggaatggga gaatctgcga ctaaaaatgt
      301 gaagtttgtc tgctcggcag gaaagggact ggactggttt accactacag gatgggctca
      361 gctttattcc atagtacagc acgattccaa cagtattctt tcaaagaaaa ccgcagtgat
      421 ctttaatttt ggagtaaacg acttgtcaaa atctgcagat tatgcggagt attataactg
      481 gattgcacca cagcttaaaa gcaaaggatg tgaactgtac ttcatgtctg taaatccggt
      541 gaatcgtctg atgcttccca atgcgggaag agcggacaga agtgaggcgg cagtcagatc
      601 attcaatcag tatatgaaag caaatctgtc ttctgcttat acttatattg atatgtattc
      661 ttatctgaaa tcaacaggat acagctttgc cagtgatcat tacggaaccg gtacggtaga
      721 tgacgggctt cactatacta caaggacata taagcgtatt tttgcaaaat gtatggattc
      781 attgagagtg cctgcctgac caaatggcag agagcatgat tttgctacac ctgacaaaag
      841 aaaaaacata gttaaatacc agattactcc ttgcgtgaaa acagggaata gtctatacta
      901 aaaggcgaga cggctgttat ctgaaacagc gtttcgtctt tttgtgtaag accagaacga
      961 tgaggagaaa gaggaaacat agatatgaag ttaacaacag taaaagagat ttacaaagag
     1021 agagaaaaat acctggataa agaggttaca gtaggtggat gggtacgaag cgtacgtgac
     1081 tccaagacat ttggatttat cgtgcttcac gacggaagct tctttgagac tcttcaggta
     1141 gtttatcacg atacaatgga gaattttgct gagatcagca aattaaacgt aggtgcagcg
     1201 atcattgtca aaggtacact ggtagcaact ccacaggcaa aacagccatt tgagatccag
     1261 gcagcagaag ttacagtaga gggtaattct gcacctgatt atccgcttca gaagaaacgc
     1321 cacagtcttg aatacttaag aaccatcaca catctccgtc cgagaacaaa cacattccag
     1381 gcagtatttc gtgtacgttc tctgtgtgcc tatgcgattc accgtttctt ccaggagcag
     1441 ggatttgttt atgtgcatac tccgctgatc acaggaagcg actgtgaagg tgcaggtgag
     1501 atgttccagg taactactct ggatatgaac aacgttccga cagatgacaa gggtgctgta
     1561 gattactctc aggatttctt cggaaaagaa actaatctga cagtaagcgg acagttaaac
     1621 tgcgagactt ttgcacaggc attccgcaat gtttatactt tcggaccaac cttccgcgct
     1681 gagaattcca acacaacccg ccatgcggca gagttctgga tgatcgagcc ggaatgcgca
     1741 tttgcagatt taaatgacaa catggatctt gcagaggcaa tgctgaaata tgtgatccgt
     1801 tatgtactgg agaatgcacc ggaagagatg aatttcttca attcttttgt tgataaggga
     1861 cttctggaca gactgaacca tgtgatcaat tctgagtttg gtcatgttac ttacacagaa
     1921 gcagtagagc ttctggagaa gaacaacgat aaatttgact acaaagtatt ctggggatgc
     1981 gatctgcaga cagagcatga acgttatctt acagaagaaa tctttaagaa acctgtattt
     2041 gtaacagatt atccgaagga gatcaaagct ttctacatga agatgaatga ggataacaag
     2101 actgttgcag ctatggactg tctggtgcct ggaatcggtg agatcatcgg tggaagccag
     2161 agagaggatg acattgagaa acttgagaaa cgtatggacg agctgggact taagaaagag
     2221 gattatgact tctatcttga tcttcgtaaa tatggttcca cacgccattc aggatttggt
     2281 cttggatttg aacgttgcgt aatgtacctt accggcatgg gcaacatccg cgacgtaatt
     2341 ccattcccaa gaactgttaa aaactgtgat ttataatttg actgatatca gtattgtggg
     2401 agcattgaag ctgcagagca atccgcagat atcaaaaatg ttatagaaag agagattggc
     2461 atgagattta ttcatcttgc agatgtacac cttggtgcag ttcctgacag gggctgtcca
     2521 tggagcagca ggcgggagga agaaatctgg gaaactttcc gccgggttat tgcgggtatc
     2581 cgcgagaatc cggtggattt gttatttata gcaggagatt tgttccaccg ccagcctctt
     2641 ctacgggaac tgaaagaagt aaataacctg ttttccacca tcccggatac ccgggtttat
     2701 ctgatggcag gaaatcatga ctatattaag gctgattctt tttaccggga ttttcagtgg
     2761 gaaaagaatg tgacattttt taagaatgaa cagcttacct gtgtgaaaga tgagaaactg
     2821 gatgtttatg tatatggact gagctatgaa catcaggaaa tagagaatcc tctgtatgat
     2881 tccatacatc ctgcagaggg agagggggtt cacattttac tggctcatgg tggagatgcg
     2941 aagcatatcc ccatgaatat caaatcaatt gctgcgtcag gatttgatta tattgcatta
     3001 ggacatattc ataaacccca gattctgatc cgggataatg cagcttatgc aggtgcgttg
     3061 gagcctgttg acagaaatga tctgggtgat catggataca tagaaggaca tctggaaaat
     3121 ggaaggctta agacaaattt tgttcctttt gcctgccgtt cctatgagca gattcttctg
     3181 atgctgcgtg aagattctac acaggcatct ctggaagata tgctgaaggc ggatctggcg
     3241 aataaaggac gcatgaatat ctacaggatc gtgatccagg gaaccagggc accggagctt
     3301 ctgctgcttc ccgagagact gaagtctttt ggctacgtaa cagaggttct ggatgaatca
     3361 aagccttcct atgatctgga agcacttcaa aaaaaatata gtggaacact gatcggtgac
     3421 tatatcagtt attttcttga aaaagacaga aatgcagtgg aggagaaggc attatattat
     3481 ggtcttcagg ctttactgga aacaagcagg tgacagacag aatatgatta tcagacagtt
     3541 aaatattaaa aactctggag aaatccatga taaaacttta gagttttctc cgggaattaa
     3601 cgttctgtat ggagagaatg aaagcgaaag agcgatagta catacgtata ttaaaaatat
     3661 gtttctcgga gatatcagtg aagcaatcta cgataatgca gtttttgctg cgaagttaaa
     3721 gagtccgaca gaacaggatc tggctcggga gctgcaaaaa tttatgaccg gctatcaggg
     3781 aactgcagac agctccatga atctgggacg ggccatgcag atgctcaaga tgtccagaaa
     3841 gggttatctg gtccaggcag acagaaaaca gaaagagaca gaacaggaga ggcagaaact
     3901 tctgaccagg atggatgata tcataagtga tctgaatgat ctgaatggaa agctggatca
     3961 gattaatgag aaagaggagt cgttacggat gcatcccgga gatgaaaatg gtgcggttat
     4021 tctggatgag agaatggcac aggcaaaggc aaaaagaaac agctttgcaa tgggaatgct
     4081 gatatcggcc actgccggca tctttggatt gattctgact gcgattatag cggacagtgt
     4141 ttcggtaagt ctgatcgtag cggttattgc agctgcgttg gtgggatttt gtggaaaaca
     4201 gcagctgaag tatgcaaggg aatttcagaa gagaatacgg atgaagaaac gctgggtatc
     4261 caggcaggag aaactcagat gcagtaaaga aaatctcaga caggactatg atgagaaaga
     4321 aacagaactt tgtaacctta aggaagaata ccgggaatac gaagaaaatt cttttctgct
     4381 tacatcggaa gagcgggagg tacaggctct gaatatggcg atggaaacca tagagagaat
     4441 gtcaggaaat attcatcttc aggtgggacg aaagctgcag atgcgtactt ctcagatttt
     4501 gagtgagatt acagatggtg aatatcagga cgtacagatg gatgcggcat cccacatgac
     4561 agtaactacc gggaacggag cgg
//
LOCUS       DBMDXG010000139         4574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4554_length_4574_cov_1.758998, whole genome shotgun sequence.
ACCESSION   DBMDXG010000139 DBMDXG010000000
VERSION     DBMDXG010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4554_length_4574_cov_1.758998"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(129..2750)
                     /locus_tag="ACZPD3_05045"
     CDS             complement(129..2750)
                     /locus_tag="ACZPD3_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR1415403.1"
                     /translation="MREIYQQTVEEVLDHVESRESGLASEQVKRSREKCGWNELAEGK
                     KKSILQIFFEQYKDFLVLILIASAVISGMLGDVESAAVIVIVITINAILGTVQTVKAE
                     QSLQSLKKLSGPEAKVLRNGVAVQLPARELVVGDVILLEAGDMIPADGRLIENASLKV
                     DESALTGESLAVEKSMDTILAEAPLGDRTNMLFSGSFVTYGRGKAVVTDVGMQTEVGK
                     IAGLLKSTSEKQTPLQANLDDFGKKLSILILIFCGILFAINVFRGEKISSAFMFAVAL
                     AVAAIPEALSSIVTIVLSFGTQKMAKEHAIIRKLQAVEGLGSVSVICSDKTGTLTQNK
                     MTVEDYYIDGKRISAAAIDAADPAQRCLLDYSILCNDSTNENGVEIGDPTETALINLG
                     SRCGIEAADVRNLYPREGELPFDSDRKMMSTLHRIDGENRMIVKGAVDRLLELTERIW
                     TKDGVREITEADKEKIQMQNQDFSMEGLRVLAFTYREIPENHILTMEDEKHLVFLGLI
                     AMMDPPREESKAAVAECIKAGIRPVMITGDHKITAAAIAKRIGILHDLSEACEGADIE
                     NMSDEQLREFVPNISVYARVSPEHKIRIVRAWQEKGMIVAMTGDGVNDAPALKQADIG
                     VAMGVTGTEVAKDAAAMVLTDDNFATIVKAVENGRNLYQNIKYAIQFLLSGNFGAILA
                     VLCASLAGLPVPFAPVHLLFINLLTDSLPAIALGVEPHSSEVMNEKPRSADESILTKD
                     FLGKIGLEGFVIGVMTMIGFLTGYYQNGALLGSTYAFGTLCLARLFHGFNCKSDHPVI
                     FTKRFFNNKWLQGAFALGSVLITAVLTVPGLHFLFKVETLNLMQLGCVYLYAFASLPI
                     IQMLKWIRMKLRKRGER"
     gene            complement(3063..4403)
                     /locus_tag="ACZPD3_05050"
     CDS             complement(3063..4403)
                     /locus_tag="ACZPD3_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415404.1"
                     /translation="MKKDSRMTEGSISRKIILFAIPLFLGNLFQQLYNTADSLIVGNF
                     LGSNALAAVSSSGNLIFLMVGFINGIAMGAGVVVARYYGAKIKDCLQKAIHTTVAFGL
                     VAGVVLTVMGMFLAPKILVLMGTPADVLPESIVYFRTYFAGSIGVVMYNIFVGILQSV
                     GDSRHPLIYLIISSCINVVLDIFFIAGLGMGVGSAALATAISQFVSAILCMFHLMRVE
                     EEYRLELRMIRFDRHMLKQIIQNGVPSGFQNSVIAIANVFVQSNINAFGKMAMAGCGS
                     YSKVEGFAFLPVTCFTMALTTFVSQNLGARQYERAKKGARFGIICSVIIAELIGIIIY
                     AAAPVLIAAFNRDPSVIHYGVMQARIIALFYCLLAFSHCIAAVLRGSGHAAVPMVVML
                     CDWCLFRVSYITVAVRLISDIRVIFWAYPLTWSISSVIFLYLFLRGKWVYGFEK"
BASE COUNT         1326 a         1183 c          763 g         1302 t
ORIGIN      
        1 ttctactatt atactcaaaa acagcagcct tgtatatagc tgctgtttcc aaaacttcag
       61 taaaattaca ttttcgccct taccataaaa agccaaaagc ttccgatgca tacctgagga
      121 aagactcact atctttcccc tctttttctt aatttcatgc gtatccattt aagcatctga
      181 ataatcggaa ggctggcaaa cgcatacaga tatacgcatc caagctgcat caggttcaac
      241 gtctccactt taaacagaaa atgaagtcca ggaaccgtca gtactgctgt aataagtact
      301 gacccaagtg caaatgctcc ctgaagccat ttattattaa agaaacgttt tgtaaagata
      361 accggatgat ccgatttgca gttaaatcca tgaaacaaac gtgccaggca aagtgttccg
      421 aaagcgtagg tacttcccag caatgcacca ttctggtaat atcctgtcaa aaatccaatc
      481 attgtcatca caccgatcac aaaaccttcc agcccgatct taccaagaaa atcttttgtc
      541 agtattgact cgtctgcaga tcttggtttc tcattcatca cctcactgct atgaggctcc
      601 acacccagtg cgattgccgg aaggctgtct gtcaaaagat tgataaacag cagatgcacc
      661 ggtgcaaagg gaaccggaag tcctgcaagc gatgcacaaa gaactgccag aatcgcgcca
      721 aagtttcctg acaaaagaaa ctggatcgca tacttgatat tctgatataa atttcgccca
      781 ttttccactg cttttacaat tgttgcaaaa ttatcatctg taagtaccat tgctgcggca
      841 tcctttgcaa cctcggttcc agtcactccc attgcgacac caatgtctgc ctgcttcaat
      901 gccggagcat cattcacacc gtctccggtc attgccacga tcatcccttt ctcctgccat
      961 gcacggacga tacggatctt gtgctcaggt gacactcttg catacacaga aatattcgga
     1021 acaaattccc tcagctgctc atcactcata ttttcaatat cagcaccctc gcatgcctcc
     1081 gacagatcat gtaagattcc aattctcttt gcaattgccg ctgctgtgat cttatgatct
     1141 cccgttatca tgaccggtct gattcccgct tttatacatt ctgcaactgc agcctttgat
     1201 tcttccctgg gcggatccat catagcaatc aggccaagaa ataccagatg tttctcatct
     1261 tccatcgtca atatatgatt ctccggaatt tcacgataag taaacgccag tacccgtaac
     1321 ccttccatcg aaaaatcctg attctgcatc tggatctttt ctttatctgc ttctgtaatt
     1381 tcccggactc catcctttgt ccaaatcctc tctgtcagtt ccagaagcct gtcaacagca
     1441 ccttttacaa tcatcctgtt ctcgccatcg attctgtgaa gtgttgacat catcttacgg
     1501 tcactatcaa aaggaagctc tccttctctt ggataaagat ttcttacatc ggcagcctct
     1561 attccgcatc tgctgcccag attgatcaat gccgtttccg tcggatctcc gatctccact
     1621 ccattttcat ttgttgaatc attacataaa atactgtaat ccaaaagaca tctctgtgcc
     1681 ggatctgctg catcaattgc agctgctgaa atcctttttc catcaatata ataatcttct
     1741 actgtcattt tgttctgtgt gagcgttcct gtcttatccg aacagataac agatacactc
     1801 ccaaggcctt ccacagcctg aagcttacga atgattgcgt gctcctttgc catcttctgt
     1861 gttccaaaag aaagaacgat tgtcacaata gagcttaacg cttccgggat tgctgcaact
     1921 gcaagcgcta ctgcaaacat aaatgcactg ctgattttct caccacggaa tacattgatt
     1981 gcgaacagaa ttccgcagaa tatcagaatc aggatggaaa gcttttttcc aaagtcatcc
     2041 agattcgcct gaagtggcgt ttgtttttca gaagtggatt tcagaagtcc cgcaatcttt
     2101 ccaacctctg tctgcatacc tacatctgtt acaactgctt taccacgccc atatgtgaca
     2161 aaactgccgg aaaacagcat gtttgtccga tcaccaaggg gtgcttctgc cagaatagtg
     2221 tccatactct tttcaaccgc aagactttca cctgtaaggg cactttcatc gaccttaagg
     2281 cttgcatttt caatcagtct tccatctgcc ggaatcatat ctcctgcttc cagcagtatc
     2341 acatctccta caaccagctc tcttgccggg agctgtacag ccacaccatt gcgcaatact
     2401 tttgcttccg gtccggaaag cttctttaaa ctctgcagag actgctcagc ctttactgtc
     2461 tgtacggttc ccaaaatcgc attaatcgta ataacgatca cgataaccgc tgcgctttcc
     2521 acatctccaa gcataccgga tatcactgcc gatgcaatta agatcagcac cagaaaatcc
     2581 ttatactgct caaagaaaat ctgtaaaata ctttttttct ttccttctgc aagctcattc
     2641 catccacact tctctctgga tcttttaacc tgttcactgg caagccctga ttccctgctt
     2701 tccacgtgat ccagaacttc ttctaccgtt tgctgataga tttccctcat tgcttttgcc
     2761 tcctttttgc aaacattcaa cagcttttaa tttttatgca atgaaggatt ttttaataaa
     2821 atgatataaa aaaagaaacc cttattgcat aacaaaaaat gcaataaaag tctcaccatt
     2881 tccttgcagt gccggatgtc tccaccgtac tgacgacact gcaaacgcag attttcaaac
     2941 gtaggttact cccctttatt gagtctaata ctataacaaa atcaatactt ctgtcaagat
     3001 attttttaaa tcatcgggca aaaaacagca gccttgtata tagctgctgt ttccgattct
     3061 atctatttct caaatccgta tacccatttt cccctcaaaa acagatacag gaaaatcaca
     3121 gagctgatgc tccaggtcag gggatatgcc cagaatatca cccggatatc tgaaataaga
     3181 cggactgcta ccgtgatata gctcacacga aacagacacc agtcacaaag catgactacc
     3241 atagggactg ccgcatggcc ggatccacgt agtactgctg caatacaatg cgaaaatgcc
     3301 agcagacaat aaaacaacgc aattattctc gcctgcatca caccataatg tattacactt
     3361 ggatctctgt taaaagcagc gatcagtacc ggtgccgccg catatatgat aatgccgatc
     3421 agctctgcaa tgatcaccga acaaatgatt ccaaatcttg cacctttttt cgcccgttca
     3481 tactgtctgg caccaagatt ctggctgaca aaagttgtca gggccattgt aaaacatgtc
     3541 actggtaaaa aagcaaatcc ctctactttt gaataggaac cacagcctgc cattgccatt
     3601 ttcccaaatg cattgatatt tgactgtaca aaaacatttg caatagcaat cactgagttc
     3661 tggaacccgg acggcacgcc attctgaata atctgtttta gcatatgcct gtcaaaacgg
     3721 atcattctta actctaaccg atattcttct tccacacgca tcaggtggaa catacaaaga
     3781 atggcactga caaactgaga aatagctgtt gccagtgcag ctgaaccaac gcccatacca
     3841 agacctgcaa taaaaaaaat atccagtaca acattgatgc atgaggagat gatcagataa
     3901 atcagggggt ggcgactatc tccgacagac tgcaggattc caacaaaaat attatacatg
     3961 acaaccccta tggatcctgc aaaatatgtt cggaaataca cgatggactc cggcagcaca
     4021 tcagcaggtg ttcccatcag taccagaatt ttaggagcca gaaacattcc cataaccgtc
     4081 agcactacac cagccacaag tccaaacgct acggttgtat ggattgcttt ctgcaggcag
     4141 tcctttattt ttgccccgta atatctggca acaacaactc cggctcccat ggcaattcca
     4201 ttgataaatc ccaccatcaa aaaaatcaga tttccggaag aactaactgc agccaacgca
     4261 ttacttccaa gaaaattgcc tacgatcagg gaatctgctg tattatacaa ctgctgaaac
     4321 agatttccga gaaatagtgg aatggcaaaa aggatgatct ttctggagat acttccctct
     4381 gtcattcttg aatccttttt cataattttt tgataccttc ctgttttttc tttattatat
     4441 cacaaaattc ttccttatat ttataatatc atgatttgaa aagaatctca aaatttactt
     4501 tcgatctgca aaatttaact gaatcaagta aatcccctgc ttcttcagta aaaagtagac
     4561 aggattactg ttgc
//
LOCUS       DBMDXG010000140         4572 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4555_length_4572_cov_3.073362, whole genome shotgun sequence.
ACCESSION   DBMDXG010000140 DBMDXG010000000
VERSION     DBMDXG010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4555_length_4572_cov_3.073362"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="ACZPD3_05055"
     CDS             <1..2214
                     /locus_tag="ACZPD3_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1108 family mobile element protein"
                     /protein_id="HIR1415405.1"
                     /translation="EEAKKKKEAKQHSKKAQVYRANEGEAPGKKKFRLKFGEGESVKT
                     EKASVVKKAGSATSVALHREISKNEDDNAAVEGVHKLEESGEGVYRLEQRSARRRKQR
                     ASRKRSRLERQEEKQTQAATRQEQKKLKKQIQKQQIKRDYAKAKRSEQTVGTATKGTI
                     DYIKKIGGKVTNFFKENRKVYISVAVLIGLMFLIITNVTSCSAVFLQNVITYTGTSYL
                     SSDQAIREAELYYTQLEANLQERINNMESEEPGHEEYRYNIGPIEHDPFILISYLSAK
                     YEEFTFEQVKPELDALFALQYRLETEAVNETVTETATVRVGESLGQVVTSGYCNCPIC
                     CGIWSGGPTASGAYPTANHTLAVDASNPFVPMGTKVVMNGVEYTVEDTGVFARYGVQF
                     DVYYDSHAAASAHGHQTWECYLADDNGSNEVEVTRTRDVDVLNVTLNSGNLMSICQDR
                     MGFFQKELFSAYNDTKGNLQMFATPVDFNWYSSVTSYYGYRIHPISGANQLHNGMDIG
                     APEGTKVMAGLTGTVTTSAYNDSYGNYVIIKDSKGYELRYAHLSTRSVSAGASVSKGD
                     EIGLVGNTGNSTGSHLHIELLKNGERLNPIFYLETGEGSSFGGNEYTSEAAQRLLEEA
                     AKYLGTPYVWGGYSPSGFDCSGFVSYCLTNSGVRNTGRLTAQGLYNICTPVSQSEAQP
                     GDLIFFTGTYDAGEPVTHIGIYVGNGQMIHCGHPVQYTSINSPYWQSHFYGFGRW"
     gene            2257..2559
                     /locus_tag="ACZPD3_05060"
     CDS             2257..2559
                     /locus_tag="ACZPD3_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HIR1415406.1"
                     /translation="MKSLEKATADYEKAKEKMEASKARYEADLKRFKAAEIAKTEAEN
                     LEIVRIIRAMDMSIPELEAFKKRMKNELPGRVEIQKEETKADDESGKNETEEENNY"
     gene            2513..3943
                     /locus_tag="ACZPD3_05065"
     CDS             2513..3943
                     /locus_tag="ACZPD3_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HIR1415407.1"
                     /translation="MMNLEKMKQKKRTITKSRVKKSLLAATTFLCLTTPLLQTQTAYA
                     AENTTPAITIEKPDGWKQGETTIAVTVDASHMPEGFSIAKIEAKAGKDGSWQDVTGSG
                     SITITGNQTVYVRVTDGDGKVYEQNRSIKCYDTEKPTLSASLTDGVLTIQGNDTVSGI
                     TAVTVNGTTYTDLKDGMLRVQLTQKDFTTKQIEITVTDGAGNTSEKYVLQNPYYEWAK
                     KQAEKQKTSSDSNGAMATTTSADATGTEKTTTSPLPQDAQASEPTDAKGTVDDRTVTG
                     IEEQLNKEGETADSVTKTATEGTKEFYTISTKSGKIFYLIIDNSKSQDNVYFLTEVSE
                     KDLMNFTLSDSVTLPEVDTVYAEPEKQAEEEKPETTETAEKDKVEDEIQMPEDKSPFG
                     TYLLIALVAVGAAAGGYYFKVYKPKHEYDDEDEMEEDEDESEDSESEKREVDDADEQE
                     ESTEPEILRDEDFDDNVLEDEEEEQE"
     gene            3944..>4572
                     /locus_tag="ACZPD3_05070"
     CDS             3944..>4572
                     /locus_tag="ACZPD3_05070"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1415408.1"
                     /translation="MKLVITEKPSVAMALASVLGARTRKDGYVEGNGYIVSWCVGHLV
                     GLCDASEYDEKYKKWRYEDLPIVPECWKHKILEGTKKQFGILKKLMRDSEVNEVICAT
                     DAGREGELIFRLVYEQAGCKKPMKRLWISSMEEQAIKDGFASLKDGSCYDSLYQSALC
                     RAKADWLVGINASRLFSVLYNQNLKVGRVQTPTLAMIVDRNQKIKEFTK"
BASE COUNT         1560 a          800 c         1203 g         1009 t
ORIGIN      
        1 gaggaagcaa aaaagaaaaa ggaagctaag cagcattcca agaaggcaca ggtctatcgg
       61 gcaaatgaag gggaagctcc tggaaagaag aaattccgct tgaagtttgg tgaaggggaa
      121 tctgtgaaaa cagaaaaagc ttctgttgtc aagaaagcag gaagtgcaac ttccgtagca
      181 ttgcaccgtg agatcagcaa aaatgaagac gataatgcgg cggtagaagg tgtccataag
      241 ttagaagaaa gcggcgaagg tgtttaccgg ctggaacaga gaagtgccag acgcaggaaa
      301 cagagggcat ccagaaaaag aagcagactt gaaagacagg aagagaaaca gacacaggcg
      361 gcaacccgtc aggagcagaa gaaactgaaa aaacagattc agaagcaaca gatcaaacgg
      421 gattatgcca aagcgaagcg aagtgaacag acagtaggaa cagctacaaa aggtacgatt
      481 gattatatca agaagattgg cggcaaagtc accaacttct ttaaagaaaa ccggaaggtg
      541 tatatcagtg tagcagttct gatcggattg atgtttctga tcataacgaa tgtcacgtca
      601 tgctctgcag tgtttttaca aaatgtgatc acttataccg gaaccagtta tctgtcatcg
      661 gatcaggcaa tccgggaagc ggaactgtat tacacacagc tggaagctaa tctgcaggag
      721 cggatcaaca acatggaatc tgaagaaccg ggacatgagg aataccgata taacatcgga
      781 cctattgagc atgatccgtt tatcctgatc agttatctgt cagcaaagta tgaagaattt
      841 acctttgaac aggtaaagcc agaactggat gctctttttg cattgcagta tcggttagaa
      901 acagaagcag tcaatgagac tgtgacagag acagcaactg taagagttgg agaatctttg
      961 ggacaggttg tgaccagtgg gtattgtaac tgcccaattt gttgtggtat ttggagcgga
     1021 ggaccgactg ccagtggagc atatccaact gccaatcata ccttagcggt agatgcttcc
     1081 aatccgtttg taccaatggg aacaaaggta gttatgaatg gtgtcgaata tactgtagaa
     1141 gataccggag ttttcgcaag atacggtgta cagtttgatg tttattatga cagtcatgcg
     1201 gcagcatctg cacatggaca tcaaacatgg gaatgttatc tggcagatga taatggaagc
     1261 aatgaagtgg aagtgaccag gacaagagat gtagatgtgc tgaatgtcac cttaaatagt
     1321 ggaaacctga tgtcaatctg tcaggacagg atgggatttt tccagaaaga actgttcagt
     1381 gcctacaacg atacaaaagg caacttgcag atgtttgcaa caccggttga ttttaactgg
     1441 tattccagtg tgaccagcta ttatggatac aggattcatc caatatcagg agcaaatcag
     1501 cttcataatg ggatggatat cggagcaccg gaagggacaa aagtaatggc agggctgacc
     1561 ggaacggtta caacttctgc ttataatgac agctatggaa attacgtcat tatcaaagac
     1621 agcaaaggtt atgagcttcg ctatgcacac cttagcaccc ggagcgtatc tgcaggagca
     1681 tctgttagca aaggtgatga aattggtctg gtaggaaaca ctggaaattc gacaggaagc
     1741 catcttcata ttgaactgtt aaaaaatgga gaacggttga acccgatctt ttatctggaa
     1801 acaggagaag gttccagttt tggtggcaat gaatacacca gtgaagcggc acagcgatta
     1861 ctagaagaag cggcaaaata tctcggaaca ccgtatgtgt ggggtggata ttccccaagt
     1921 ggattcgatt gttccggatt tgtaagctac tgtctgacaa actctggtgt aaggaataca
     1981 ggaagactaa cagcacaggg actgtacaac atttgtacac cagtatccca gtcggaggca
     2041 cagccgggag atttgatttt ctttactgga acttacgacg caggggagcc ggtaacgcat
     2101 atcggaatct atgtaggcaa tggtcagatg atccattgtg gacatccggt gcagtatacg
     2161 tccatcaatt ccccatactg gcagagccat ttctatggat ttggacgatg gtaagaaaaa
     2221 agggcggctg catcagtcgt ccggaaagga gaaccgatga aaagtcttga aaaagcaacg
     2281 gcagattatg agaaagccaa agagaaaatg gaagcatcca aagccagata tgaagcagat
     2341 ttaaagcggt tcaaggctgc ggagattgca aaaacagaag cggaaaatct ggaaattgta
     2401 agaattatcc gtgcaatgga tatgagtatt ccggaattgg aagctttcaa aaagagaatg
     2461 aaaaatgaac tgcccggcag ggtagaaata cagaaggagg aaacaaaagc cgatgatgaa
     2521 tctggaaaaa atgaaacaga agaagagaac aattactaaa agcagggtaa aaaagagtct
     2581 gcttgcagca acgacatttc tttgcctgac aacacctttg ctccagacac agactgctta
     2641 tgcagctgag aataccacgc ctgcaatcac gattgagaaa ccggatggat ggaaacaggg
     2701 agaaacaacc attgctgtta cggtagatgc cagccatatg ccggaaggtt tctcaatagc
     2761 aaagattgaa gcaaaagcag gaaaagatgg aagctggcag gatgtaactg gaagcggaag
     2821 tatcacgatc acaggaaatc agacagtgta tgtgcgtgtg acagatggtg atggaaaggt
     2881 gtatgagcag aatcgttcta ttaagtgtta tgacacggaa aagccgacac tctctgcttc
     2941 cctgacagat ggtgtgctga cgattcaggg gaatgataca gtatccggga ttactgcagt
     3001 aacagttaat ggcaccactt atacagatct gaaggatgga atgctgagag tgcaactgac
     3061 acagaaagat tttactacaa aacagattga gattacggta acagacggtg ccggaaatac
     3121 ttctgaaaag tatgtgttgc agaatcctta ttatgaatgg gcaaagaagc aggcagagaa
     3181 acagaaaacc tccagtgaca gcaatggtgc aatggcaact acgaccagtg cagatgcaac
     3241 gggaactgaa aaaacaacga cttctccgct tccgcaggat gcacaagctt ctgagccaac
     3301 agatgcaaaa ggaactgtgg atgatcggac agtgacaggt attgaggaac agcttaataa
     3361 agaaggcgag acagcagatt ctgttactaa aacagctacg gaaggcacaa aagagtttta
     3421 taccatttca actaagagtg gtaagatttt ttatctgatc atcgacaatt caaaatctca
     3481 ggataatgtg tatttcctta cagaagtcag cgagaaagat ctgatgaact tcacactttc
     3541 tgattccgtg acacttccgg aagtagatac tgtttatgca gaaccagaga aacaggctga
     3601 agaagaaaag ccggaaacaa ctgagacagc tgagaaagat aaggtagagg atgaaatcca
     3661 gatgccagaa gataaaagtc catttggaac gtatcttctg attgcactgg ttgcagtagg
     3721 agctgcggca ggaggatatt acttcaaagt ctataaacca aagcatgaat atgatgatga
     3781 ggatgagatg gaagaggatg aagatgaatc cgaagattca gagtcagaaa aacgggaagt
     3841 agacgatgca gatgagcagg aagaaagtac agaaccggaa attctgagag atgaagattt
     3901 tgacgataat gtgctggaag atgaagaaga ggagcaggag taaatgaaat tagtgattac
     3961 agaaaagcca tcggttgcga tggcactggc ttccgtgctt ggagccagaa caagaaaaga
     4021 tggctacgtg gagggaaacg gttatatcgt ttcctggtgc gtaggacatc tggttggatt
     4081 atgtgatgca tcggaatacg atgaaaaata taaaaaatgg agatatgaag atctgccgat
     4141 tgtgccggaa tgttggaaac acaagatttt ggagggcaca aagaagcagt tcggaatctt
     4201 aaagaaactg atgagagatt ctgaagtgaa tgaagtaatc tgtgccactg atgcaggacg
     4261 tgaaggtgag ctgatcttcc gtctggtgta tgagcaggca ggatgtaaaa agccgatgaa
     4321 gcgactctgg atctcatcta tggaagaaca ggcaattaaa gatggatttg cgtcattaaa
     4381 agacggttcg tgttatgaca gtctctatca gtcagcactt tgcagggcaa aggcagactg
     4441 gctggtggga atcaatgcga gccgtctttt ttctgttctg tataaccaga atctgaaagt
     4501 tggaagagta cagacaccaa cacttgccat gatcgtggat cgaaatcaga agatcaagga
     4561 atttaccaaa ga
//
LOCUS       DBMDXG010000141         4563 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4570_length_4563_cov_1.417078, whole genome shotgun sequence.
ACCESSION   DBMDXG010000141 DBMDXG010000000
VERSION     DBMDXG010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4570_length_4563_cov_1.417078"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="ACZPD3_05075"
     CDS             complement(<1..510)
                     /locus_tag="ACZPD3_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR1415409.1"
                     /translation="MDTVQKTAAGSQQRILEFDLASELHHGMLVSNLAYAVAEEMGLP
                     HEQCYELAIAGMLHDIGKLKLTSYINGQEQDPLVIEELKYVRMHSALGYEELKGQGYS
                     DFVLESILYHHENYDGSGYPSNKAGDDIPIGARILRVCDVYAALISDRPYRRGFDISS
                     VMELMIDEVK"
     gene            complement(528..2063)
                     /gene="hemZ"
                     /locus_tag="ACZPD3_05080"
     CDS             complement(528..2063)
                     /gene="hemZ"
                     /locus_tag="ACZPD3_05080"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HIR1415410.1"
                     /translation="MKRGIIHLCKIGILFKNRDFEHDVYELIKAFYPEAEIHTLYENG
                     EAEYDLRFSVERDNDSYIIKYERTENLSKQETEQKGVISADILSKENAGCGIQDAHAL
                     RKENKDNIKYALYQLLVKLTGRTLPWGNLTGIRPAKLAMGMIESGMKNTEAAREMRER
                     YLVSPQKTALAITIANREREILKDIDYENGYSLYIGIPFCPSICLYCSFSSYPLKVWE
                     KRTDEYVEALCREVRETALMMKGRKLDTIYIGGGTPTTLLPHQIRKLLDTVGEAFGYE
                     GLAEFTVEAGRPDSITREKLMAIREYPVTRISVNPQTMNQETLDIIGRRHTVEQTKEA
                     FHLARELGYDNINMDLIVGLPGEDIHMVERTLEQVRELAPDSITVHSLAVKRAARLNI
                     FKEKYQEMSFENNQEIMDLTMKTAYEMGMGPYYLYRQKNMKGNFENVGYAKVDKAGIY
                     NILIMEEKQPIIALGAGGSSKLVFDHGQRIERVENVKDVSNYISRIDEMIERKRTAIA
                     TWL"
     gene            complement(2035..2664)
                     /locus_tag="ACZPD3_05085"
     CDS             complement(2035..2664)
                     /locus_tag="ACZPD3_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1415411.1"
                     /translation="MKNLELQKCILGPVYTNCYLLKNKETGELIIVDPADCLEKIEMK
                     ISRMNGKPVAILLTHGHFDHILAAQAVKEKYNIPIYACRQEEEMLREPSINMTVHYGQ
                     GCSIVPDVFLEDLDVIRLAGFSVQMIHTPGHTKGSCCYYLKDEGVLFSGDTVFYGSVG
                     RTDFPGGSTAEIVRSLHKLVDSLPEETEVFPGHDASTTIGYEKRYNPFV"
     gene            complement(2729..>4563)
                     /locus_tag="ACZPD3_05090"
     CDS             complement(2729..>4563)
                     /locus_tag="ACZPD3_05090"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR1415412.1"
                     /translation="AMAKDIRVILIKLADRLHNMRTLQYMRPEKQQEKARETMDIYAP
                     IAMRLGISKIKVELDDLSLKYLKPDVYYDLVHKVALRKSEREQFVGAIVKEVKKHMDD
                     ANIKAQVDGRVKHFFSIYKKMVNQDKTIDQIYDLFAVRILVDTVKDCYAALGVIHEMY
                     KPIPGRFKDYIAMPKPNMYQSLHTTLIGPNGQPFEIQIRTYEMHRTAEYGIAAHWKYK
                     ESSDGKAPVGKSEEEKLNWLRQILEWQRDMSDNKEFMSLLKNDLDLFADSVYCFTPQG
                     DVKTLPSGSTPVDFAYSVHSAVGNKMVGARVNGKLVPIEYEIKNGDRIEIITSQNSQG
                     PSRDWLKLVKSTQAKNKINQWFKKELKEDNILKGKEMLAQYARAKGFKIANYTKTQYL
                     EAVLRKYGFRDWDSVLAAIGHGGLKEGQVFNKLVEAYDKENKKNLTDEQVLEAASETQ
                     EKLHIAKSKSGIVVKGIHDVAVRFSKCCNPIPGDEIVGFVTRGRGITIHRTDCVNVLN
                     MSETDRTRLIEAEWQQPDTKEKEKYMAEIQVYANNRTGLLVDLSKIFTERKIDLRSIN
                     SRTSKQEKATISMSFEIGSKEELRSLIEKIRQVESVIDVERTTG"
BASE COUNT         1160 a         1181 c          793 g         1429 t
ORIGIN      
        1 ttttacttca tcgatcatca actccataac tgaagaaata tcaaatcctc ttctgtatgg
       61 tctgtcagag atcaatgctg catatacatc gcagactctg agtatccggg cacctatggg
      121 tatatcatca cctgctttat tggacggata tccactacca tcataattct catgatggta
      181 caggatactc tccaatacaa aatccgaata tccctgccct ttaagttcct catatcccaa
      241 tgcagaatgc atccggacat acttcagttc ctcgatcacc agaggatcct gctcctgtcc
      301 gttaatataa cttgtcagct tcagctttcc tatatcatgg agcattcctg ctattgcaag
      361 ctcataacac tgttcatgcg gcagtcccat ctcctctgca accgcataag ccagattact
      421 taccagcatt ccatgatgca gttcagaagc aaggtcaaat tccaggattc tctgctgtga
      481 acctgctgct gtcttttgta ctgtgtccaa aatgttacct ccacatccta cagccaggta
      541 gctattgctg tccgttttct ctcgatcatc tcatcaatgc gggaaatata attagaaacg
      601 tcttttacat tctccacacg ttcaattctc tgtccatggt caaagaccag cttggaagaa
      661 ccgcctgccc ccagtgcgat gataggctgt ttttcctcca taatcagtat attgtaaatt
      721 cccgctttgt caacctttgc atagccaaca ttttcaaaat ttcccttcat attcttctgt
      781 ctgtacagat aataaggacc cattcccatt tcatacgcag ttttcatggt cagatccata
      841 atctcctgat tattctcaaa agacatttcc tgatatttct ctttaaaaat attcagcctt
      901 gctgcacgtt tcactgccag tgaatgtacg gtaatactgt ccggtgccag ttcacgaacc
      961 tgttcaagag tccgctctac catgtgaata tcctcacccg ggagtcctac gatcagatcc
     1021 atgttaatgt tatcgtatcc cagctctcgt gccagatgaa acgcttcttt tgtctgctcc
     1081 acagtatgtc tgcggccgat aatatccagg gtttcctgat tcatagtctg tggatttacg
     1141 gaaattcttg ttaccggata ctccctgatc gccatcagct tttcacgtgt aatactgtcc
     1201 ggtctgccgg cttccacagt aaactctgcc agtccttcat agccaaaagc ttctcccact
     1261 gtatccagga gcttacggat ctgatgtgga agcagtgtag tcggggtacc gccgccaata
     1321 tagatggtgt ccagttttct tcccttcatc ataagagcag tctctcttac ctcacggcag
     1381 agagcttcca catattcatc tgttctcttc tcccacactt tcagcggata ggagctgaag
     1441 gaacaataca gacagatact tgggcaaaac gggattccaa tataaaggct gtatccattt
     1501 tcatagtcaa tatccttcag aatctctctt tccctgtttg caatagtgat ggcaagcgct
     1561 gttttctgcg gactcaccag atatctctca cgcatctccc gggctgcttc cgtatttttc
     1621 attccggatt cgatcattcc cattgccagt ttggcaggac gtattcctgt cagatttccc
     1681 catggaagag ttctgcccgt cagttttacc aacaactgat acagagcata tttgatatta
     1741 tctttattct cttttctcag agcatgagca tcctgtattc cacagcctgc attctcttta
     1801 gaaagtatgt ctgcggaaat tacccctttt tgctctgttt cctgtttcga cagattctct
     1861 gtcctttcgt attttatgat ataactgtca ttatcccgtt ccacactgaa gcggaggtca
     1921 tactctgctt caccattttc ataaagagta tgtatctccg cttcgggata aaacgctttg
     1981 atcagttcat aaacatcatg ttcaaaatct ctgttcttaa acagaatgcc tattttacac
     2041 aaatggatta tacctctttt cataaccaat agtggtagat gcatcatgtc cggggaaaac
     2101 ctcggtctct tcaggaagcg aatctaccag tttatggaga cttctcacaa tttctgctgt
     2161 acttccaccc ggaaaatccg ttcttccaac agagccatag aacactgtat caccgctgaa
     2221 caatacacct tcatctttca gataataaca gcagcttcct ttggtatgtc ccggtgtatg
     2281 gatcatctga acggaaaatc ccgccagtct gataacatcc agatcctcca gaaatacatc
     2341 cggcacaata gaacatccct gtccataatg tacagtcata ttgatcgaag gttcgcggag
     2401 catctcctct tcctgacgac aggcataaat cggaatatta tatttctcct ttactgcctg
     2461 tgcagccaga atgtggtcaa aatgtccatg ggtaagaaga atcgccaccg gttttccatt
     2521 catccgggaa atcttcattt caatcttctc cagacaatct gcgggatcaa caatgatcag
     2581 ttctccggtt tctttattct taaggagata gcaatttgta tatacaggtc ctaatataca
     2641 tttctgtaat tctaaattct tcatgttatt cctttctgtc aatctgcagc agttccataa
     2701 aataatgttc cagcagattt ttccatgatc agcctgtagt tctctctacg tcaatgacac
     2761 tctctacctg acggatcttc tcgatcaggg aacgcagttc ctctttgctt ccaatctcaa
     2821 aactcatgga aatagttgcc ttctcctgct tgcttgtacg gctgttgata cttcgcaggt
     2881 cgatcttacg ttctgtaaat attttagaaa gatccacaag aagccctgta cggttatttg
     2941 cataaacctg aatctcagcc atatacttct ctttttcctt ggtatcaggc tgctgccatt
     3001 ccgcctcgat caatcgtgtc ctgtccgtct cagacatatt cagcacattg acgcaatctg
     3061 tacgatggat tgtgattcca cgtcctcttg taacaaagcc tacaatctcg tcacccggga
     3121 tcggattaca gcacttggaa aaacggactg ccacatcatg gattcctttt accacaattc
     3181 cactcttgct cttggcaatg tgaagcttct cctgtgtctc agaagcagcc tcaagaacct
     3241 gctcatctgt cagattcttc ttattttcct tatcataagc ctctaccagc ttattgaaaa
     3301 cctgaccttc tttcaatccg ccatgaccaa ttgcagcaag tactgaatcc cagtccctga
     3361 aaccatattt acgcaaaaca gcctccagat actgggtttt tgtataattt gcgatcttaa
     3421 atccctttgc acgcgcatac tgcgcaagca tctccttgcc cttaaggata ttatcttctt
     3481 ttaattcctt cttgaaccac tggttaatct tgttctttgc ctgagtactc ttaaccagtt
     3541 tcaaccagtc gcggcttggt ccctgagaat tctgtgaagt aatgatttca atacggtcgc
     3601 cgtttttgat ctcatattca atcggaacca gcttaccgtt aactctggca cctaccatct
     3661 tatttccgac tgcactgtga acactgtacg caaaatccac cggtgtagaa ccgcttggaa
     3721 gtgttttaac atctccctgt ggagtgaaac agtaaacact gtccgcaaaa agatccaggt
     3781 cattcttcag aagactcatg aattctttgt tatcagacat gtctctctgc cattccagaa
     3841 tctgacgcag ccagttcagt ttctcttcct cactcttacc tacaggagcc tttccgtcag
     3901 acgactcctt atatttccag tgtgcagcga taccatattc tgcagttctg tgcatttcat
     3961 aagtacggat ctggatttca aagggctgtc cgttgggtcc gatcaatgtg gtatgcaatg
     4021 actgatacat attgggtttc ggcatggcaa tataatcttt aaaccgtccc ggaattggct
     4081 tatacatctc atggatcaca ccaagggcag cataacaatc cttcactgta tctacaagaa
     4141 tacggacagc aaataaatca taaatctgat caatagtctt atcctgattt accatcttct
     4201 tgtagatgct gaaaaagtgt tttacacgtc cgtccacctg cgccttaata ttggcatcat
     4261 ccatatgttt cttaacttct tttacaatcg cacctacaaa ctgttctctc tcgcttttac
     4321 gcagggcaac cttatgcacc aggtcataat atacatccgg cttaaggtat ttcagcgaca
     4381 ggtcatccag ctctaccttt atcttggaaa tacccagacg catggcaata ggagcataaa
     4441 tatccatggt ctctctggcc ttttcctgct gcttttcagg acgcatatac tgtaaggtac
     4501 gcatattatg aagacggtct gcaagcttga tcagaataac tctgatatcc ttagccatgg
     4561 caa
//
LOCUS       DBMDXG010000142         4561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4571_length_4561_cov_1.728924, whole genome shotgun sequence.
ACCESSION   DBMDXG010000142 DBMDXG010000000
VERSION     DBMDXG010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_4561_cov_1.728924"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="ACZPD3_05095"
     CDS             complement(<1..266)
                     /locus_tag="ACZPD3_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415413.1"
                     /translation="MKELLQKIEKRMDDFTVKKKLYILYIFCVLLPLIITDSVVFLII
                     RNSEMEKQKHDMTNIANAVSYNISSVVNNAGEIIPLNTLEYPLKL"
     gene            478..1359
                     /locus_tag="ACZPD3_05100"
     CDS             478..1359
                     /locus_tag="ACZPD3_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1415414.1"
                     /translation="MIEHLDGIHETVTYKENSSLRLFVNTDCEDYPIHWHTPMEIIMP
                     LEGTYTVFLGDKPVLLQEGDIIFICPGILHSLVAPENGKRIILQIEWSVVSKIRDLNS
                     ILALISPVLTLTPQTAPDIYSDIYHLICQILSEYEEDSFLSEAVIYARMLDILSLIGR
                     YQAFASCRFDAGPQKQKEYLDRFLSICNYIDEHCTEDLSLDQVAKTAGFSKYHFTRLF
                     KQFTNTTYYKYLNRKRIDHAMLLLSDPDIPVTEAALQSGFSSLSTFLRVFRSVKQCTP
                     TEFRDMYIHPEKQQKNP"
     gene            complement(1356..2921)
                     /locus_tag="ACZPD3_05105"
     CDS             complement(1356..2921)
                     /locus_tag="ACZPD3_05105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR1415415.1"
                     /translation="MVTANNPILSGFYPDPSICRAGDDYYIVNSSFVYAPGVPIFHSR
                     DLAHWEQIGNILNRKEQLPVEGSEISRGIFAPTIRYHEGLFYMITTNVSYGGNFIVTA
                     KKPEGPWSDPYYLGEEAAGIDPSLFFDEDGKCYYCGTRPNPEGVRYNGDWEIWVQELD
                     LNTMKLTGESMTIWKGAVKDCIWPEGPHIYKIGEYYYLMHAEGGTGPEHSISVARSRK
                     LFQWFEGCPRNPIFTHRNLGMDYPVIYAGHGDLVDDGHGNWYVVMLASRPCRKHSSMG
                     RETFLAKVIWENGWPVIAPGVGHLEEQVEIPMEEYRFAQEVTKCDQIHFWDKELDQRL
                     IGIGGCDETIYSLKEKEGMLRLYPRKESIAEAAYCSYLGLRQKSYRFEAETGVFFVPE
                     NEKETAGLVLYQNYENHLRMEIAKKQEQKVFRVVTCIKGTETKAAEVLLPGETEQLLK
                     IRLQCHDQTAKVWIASENGWECAAEEISLLPYTTEEAGGFVGCTIGMYASSNGMESKN
                     HADFAWFTCSSRN"
     gene            complement(2933..>4561)
                     /locus_tag="ACZPD3_05110"
     CDS             complement(2933..>4561)
                     /locus_tag="ACZPD3_05110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR1415416.1"
                     /translation="QFVYESDGRLINSAQIAGNITDQEELALLENVEGFRKLVHSIGI
                     SVELDDPRESVEFVFQMYGKKDLYGGGTNLKTKLPGDGMERKIDLSDYTWTEDDDIPG
                     QIKFIFQTPELLGKASVRLYLNDGYDAPKETAEEKIDIRSEEYREMIQRSLMNFGNTC
                     RIQRVIEKARDGKEVTLAYIGGSITQGAGAIPIHTKCYAYQSCQLFQKRFAAQDNVRL
                     IKAGVGGTPSELGMIRFDRDVLRGEEQPDLVVIEFAVNDEGDETKGDCYESLVRKVLN
                     LPWKPAVVLLFSVFANDWNLQERLKPVGYCYDLPMVSIRDAVSPQFQMPKGQGRVLTK
                     NQFFYDMFHPNNMGHTIMADCLQYLFEQCDLRNQCGGETLERQMKAEERLKECMSEPP
                     AIGKSFETVRLLDRKDRYRGAEIDEGGFTAVDRELQSVEMDAELTPVPQFPYNWMYDG
                     TNSDKNYFEMTITCRSLLLVFKDSGEIYTGKAEISVDGGYCMTADPHINNWLHCNAVI
                     LFQEEESKKHIVRITIPEEDRNKQFTILGFGYVQ"
BASE COUNT         1119 a         1187 c          799 g         1456 t
ORIGIN      
        1 agtttaagag gatattctaa tgtgttcagc ggtataattt ctcctgcatt atttaccacg
       61 ctgctgatgt tgtagcttac cgcatttgca atgttggtca tatcatgttt ctgcttttcc
      121 atctcggaat ttcttatgat taaaaaaacc acgctgtccg taatgatcag cggaagcagt
      181 acgcaaaaaa tatataaaat gtataatttc tttttgaccg taaagtcatc catccgtttt
      241 tcaatttttt gaagcaactc tttcattctc agaacctctc acgtgaatca gggagcagac
      301 actgcctgct cccttctgga ttttccaatg tcatctttca cacagttatc ttatcatatc
      361 cagatttaga ttggaattct gttttcagtt ctcttctttc aattttttct cttgtgatct
      421 ctgttttact gtattatgat aatatcaaat ttacagcagt cctccggagg caatattatg
      481 attgaacatt tagacgggat tcacgaaacc gtaacgtata aagagaacag cagtctgcgg
      541 ctttttgtaa acacagactg cgaggactat cccattcact ggcatactcc catggaaatc
      601 attatgcctc tggaagggac ttatacggtt ttcctcgggg ataaacctgt tcttcttcag
      661 gagggcgaca ttattttcat ctgccctggg atccttcact ctcttgtggc accggaaaac
      721 ggaaaacgaa ttattctgca gattgaatgg tcggttgtgt caaaaatccg cgatctcaat
      781 tccatcctcg cgctgatttc cccggttctc actcttactc ctcagaccgc tcctgacatc
      841 tattccgata tttatcatct tatctgccag attctttcgg aatatgaaga ggattccttt
      901 ctttcggaag ctgttatcta tgcgcgcatg cttgatattc tttctctgat cggcagatac
      961 caggcctttg catcctgccg ttttgatgcc ggaccgcaaa agcagaaaga atatctggat
     1021 cgttttcttt ccatctgcaa ttatattgat gaacactgca cggaagatct ttctcttgac
     1081 caggttgcaa aaacagcagg cttcagcaaa taccacttta ccagactgtt taagcagttt
     1141 acaaatacca cctattacaa ataccttaat cgcaaaagaa ttgatcatgc catgctgctc
     1201 ttatccgatc ccgatattcc tgtcacggag gcagcacttc agtctggttt ctccagtctt
     1261 tccacctttc ttcgcgtctt ccgttctgtc aaacaatgta cgcctacgga attccgcgat
     1321 atgtatattc atccggaaaa gcagcagaaa aatccctaat tcctgctgct gcaggtaaac
     1381 catgcaaagt ctgcatggtt tttgctttcc attccattag aagaagcata catgccgatg
     1441 gtacatccga caaaaccacc tgcctcttct gttgtgtacg gaagaagact gatttcctct
     1501 gccgcacatt cccagccatt ttctgacgca atccatactt ttgccgtctg atcatggcat
     1561 tgcaggcgta ttttcagcag ttgttccgtc tctcctggca gtaaaacttc tgctgctttc
     1621 gtttctgttc ctttgataca cgttacaaca cgaaaaactt tctgttcctg cttttttgcg
     1681 atttccatcc tcagatgatt ctcataattc tggtaaagaa ccagacctgc cgtttccttc
     1741 tcgttttccg gcacaaagaa aactcctgtt tccgcttcaa agcgatagct tttctgccgt
     1801 aatccaagat aggagcaata ggctgcttct gcaatacttt cttttcgggg atacaaccgc
     1861 agcattccct ctttttcttt cagcgaataa atggtttcat cacacccgcc aattccaatc
     1921 agtctctgat ccaactcttt atcccagaaa tgaatctgat cacattttgt aacttcctgt
     1981 gcaaagcgat attcttccat tgggatttcc acctgctctt ccagatgtcc cactccaggc
     2041 gcaatgaccg gccatccatt ttcccatatc actttagcaa gaaatgtctc tctgcccata
     2101 ctgctgtgtt ttctgcacgg ccgtgaagca agcattacta cataccagtt tccatgtcca
     2161 tcatctacaa gatctccatg gcctgcataa atcaccggat aatccattcc cagattgcga
     2221 tgtgtaaaaa tggggttcct cgggcatcct tcaaaccact gaaacagttt tctgcttctg
     2281 gcaactgaga tactgtgttc cggacctgta ccgccctctg catgcatcag gtaataatat
     2341 tctccgattt tgtaaatatg cggcccttcc ggccagatgc agtcttttac cgcacctttc
     2401 cagattgtca tgctttctcc tgtcagtttc atggtgttca gatcaagttc ctgtacccag
     2461 atttcccagt ccccattata tcgcacacct tccgggtttg gtcttgttcc gcagtaatag
     2521 cattttccat cctcatcaaa aaacaggctt ggatcaattc ctgctgcttc ttcaccaaga
     2581 taatacggat ctgaccaggg accctccggc tttttcgctg taacaataaa atttccgccg
     2641 tagcttacgt ttgttgttat catataaaat aatccttcat gataacgaat cgtcggtgca
     2701 aaaatacctc ttgagatttc acttccctct actggaagct gctccttccg gttcagaata
     2761 tttccgatct gttcccagtg tgccagatct ctgctgtgaa aaatcggaac tcccggggca
     2821 taaacaaaac ttgaatttac aatatagtaa tcatcccccg ccctgcaaat cgacggatcc
     2881 ggataaaaac cggataaaat tggattattt gccgttacca taactttctc ctttactgta
     2941 catacccgaa tccaagaatc gtaaactgtt tattcctgtc ttcttctggt attgtaattc
     3001 tcacgatatg tttcttactc tcttcctcct ggaaaagaat gaccgcattg caatgcagcc
     3061 agttattgat atgcggatcc gctgtcatac agtatcctcc atctacggaa atctctgctt
     3121 ttcccgtata aatttcaccg gaatctttaa aaacaagaag cagacttctg caggttatcg
     3181 tcatttcgaa atagttttta tcggaattgg tgccatcata catccagtta tacgggaact
     3241 gcggcacagg tgtaagttct gcgtccattt cgacgctctg cagttccctg tccacggctg
     3301 taaatccacc ttcatcaatt tcggctcctc tgtatctgtc ttttcgatcc agcagacgaa
     3361 ctgtttcaaa actctttccg attgcgggtg gctcgctcat gcattctttc agtcgttctt
     3421 ccgccttcat ctgtctttcc agtgtttcac ccccacattg attcctgaga tcgcattgtt
     3481 caaacaaata ctgcagacag tccgccataa tcgtgtgccc catattgttt ggatgaaaca
     3541 tatcataaaa gaactgattt tttgtcagaa cccgtccctg tccttttggc atctgaaact
     3601 gcgggcttac ggcatcccgt atactgacca tcggaagatc atagcagtat cccaccggtt
     3661 tcaggcgttc ctgaagattc cagtcatttg caaaaacaga aaacagaagc actacagcag
     3721 gcttccaggg aagattcagc actttacgta caaggctttc atagcagtcc ccctttgttt
     3781 catccccttc atcattaaca gcaaattcga tcactacaag atccggctgt tcctctccgc
     3841 gcagaacatc tctgtcaaag cggatcatcc ccaactcaga aggggtgcct cccactcctg
     3901 cttttatcaa tctgacatta tcctgtgctg caaagcgctt ctgaaataat tggcaggact
     3961 gataggcgta acattttgta tggatcggaa ttgctcccgc cccctgcgtg atagatccgc
     4021 cgatatatgc aagcgtaact tcttttccgt ctctggcttt ttcaattact ctctgtatcc
     4081 ggcaggtatt tccgaaattc atcagagatc tttgtatcat ttccctgtat tcttccgaac
     4141 gaatatctat tttttcttct gcggtttctt tcggtgcatc atatccatca ttcaaataaa
     4201 gtcttacact tgcttttccc agcaattccg gtgtctgaaa gataaatttt atctgccccg
     4261 gaatatcatc atcttctgtc catgtataat cggacagatc tatttttctc tccattccat
     4321 ctccgggcag tttggttttc agattcgttc cccctccata gagatccttt tttccataca
     4381 tctgaaacac aaattcaaca gactctctgg gatcatccag ttccacagag attccgatgc
     4441 tgtgtaccag ttttcgaaat ccttctacgt tctccagtaa agccagttcc tcctgatctg
     4501 tgatatttcc ggcaatctgt gcactgttga tcagtcttcc gtcactctca tacacgaact
     4561 g
//
LOCUS       DBMDXG010000143         4550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4581_length_4550_cov_1.641942, whole genome shotgun sequence.
ACCESSION   DBMDXG010000143 DBMDXG010000000
VERSION     DBMDXG010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4581_length_4550_cov_1.641942"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="ACZPD3_05115"
     CDS             <1..812
                     /locus_tag="ACZPD3_05115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1415417.1"
                     /translation="NLSEIKRMPKSILVNGVHYYMRQELSKGDHLQVRICETQNSEKI
                     PPTKLPLDIIYEDEDILVINKPADMPVHPSAGNYENTLANGIAWYFAEKGEDFVYRCI
                     NRLDRDTTGALILAKNPLSAAILSVQMKKRQILRTYLALVDGLLPDSGTINAPIARME
                     GSVITREVNFGTGESAITHYERLAAGKEYSLAELHLETGRTHQIRVHMKYIGHPLPGD
                     YLYNPDYRRINRQPLHSYQLEFTHPTTGKVMLFTAPLPIDFISAFYSNPLK"
     gene            complement(906..1670)
                     /locus_tag="ACZPD3_05120"
     CDS             complement(906..1670)
                     /locus_tag="ACZPD3_05120"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR1415418.1"
                     /translation="MILAIDVGNTNIVVGCIDDRKTYFIERLSTNRTKTELEYAVDLK
                     NVLDIYHIKKAEIEGCIISSVVPQITNIVKLAAEKILKKNAIVLGPGVKTGLNIMMDN
                     PGQLGADQVADAVAGIAGYPVPLILIDMGTATTASVVNSKKQYVGGMILPGVGVSLDA
                     LTARASQLSGISIDAPRHVIGKNTIECMKSGVLYSNAAALDGIIDRIEEELGEKATVV
                     ATGGLAKKIVPHCKREIILDEELLLRGLLIIYEKNK"
     gene            complement(1708..2916)
                     /gene="coaBC"
                     /locus_tag="ACZPD3_05125"
     CDS             complement(1708..2916)
                     /gene="coaBC"
                     /locus_tag="ACZPD3_05125"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR1415419.1"
                     /translation="MTLEGKTVLLGVTGSIAAYKIAYLASALKKRHADVHVLMTENAT
                     NFINPITFETLTGNKCLVDTFDRNFQFQVEHVSIAKKADVVMIAPASANVIGKLAHGI
                     ADDMLTTTIMACKCKKFISPAMNTNMFENPVVQDNLKILEHYGYEVIAPACGYLACGD
                     TGAGKMPEPETLLAYIEREAACEKDLKGKKILVTAGPTQESVDPVRYLTNHSSGKMGY
                     AIAKAAMLRGADVTLVSGRTSIEPPMFVNLIPVVTARDMYEAVTSVSNEQDIIIKAAA
                     VADYRPARISEEKVKKSDGQMSIELERTDDILKFLGEHKQHGQFLCGFSMETQNVIDN
                     SRAKLAKKNLDMVAANNVKVEGAGFQGDTNVLTLITQDEEISLPLMSKEDAAFRILDK
                     ILSLMQDSVC"
     gene            3427..3990
                     /gene="mnmM"
                     /locus_tag="ACZPD3_05130"
     CDS             3427..3990
                     /gene="mnmM"
                     /locus_tag="ACZPD3_05130"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     5-(aminomethyl)-2-thiouridylate-methyltransferase MnmM"
                     /protein_id="HIR1415420.1"
                     /translation="MKNYQITQWCAAFIRQQVQEGDFCIDATMGNGNDTLLLSQLCGE
                     SGKVLAFDIQEQALTATQKRLNAGHVPENYRLLLESHANMAEYATPDSVSCIVFNFGY
                     LPGGDHSLATRGKTSIQALTQALTLLKKGGMISLCIYSGGDSGFEERDQILDWLKNLD
                     PHQYLVIKSEYYNRPNNPPIPVLIIKN"
     gene            complement(4112..>4550)
                     /locus_tag="ACZPD3_05135"
     CDS             complement(4112..>4550)
                     /locus_tag="ACZPD3_05135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR1415421.1"
                     /translation="DADTAPITVTNFVKLAQEGFYDGLTFHRIMDGFMIQGGDPNGDG
                     TGGSEENIKGEFSNNGVDNDISHTRGTISMARASDPDSASSQFFIVQADSTFLDGDYA
                     GFGHVTEGMDIVDKICEDAKPTDDNGTIPSDQQPVIEKITITD"
BASE COUNT         1235 a         1087 c          859 g         1369 t
ORIGIN      
        1 agaatctgtc ggagatcaaa agaatgccga aaagtatcct tgtaaatggg gtacattatt
       61 atatgcgtca ggaactttcc aagggagacc acctgcaggt gcggatctgt gagacacaaa
      121 attctgagaa aatcccaccg acgaaacttc cactcgatat catttatgaa gatgaggata
      181 tccttgtgat aaacaagcct gcagatatgc ctgtgcaccc ttctgcggga aattatgaaa
      241 atactctggc aaatggaatc gcctggtatt ttgcagaaaa aggagaagat tttgtttacc
      301 gctgtatcaa ccggctggac cgggatacca ccggtgctct gatccttgcc aaaaatcccc
      361 tcagcgcagc gattctgtca gttcagatga aaaaacgtca gattctcagg acttatctcg
      421 cacttgtaga cgggcttctt cctgactccg gaacgatcaa cgctcctatc gcacgaatgg
      481 aaggctccgt gatcaccagg gaagtaaatt tcggaaccgg ggagtctgca ataactcatt
      541 atgaacggct ggcggcagga aaagaatatt ctctggccga acttcatctg gaaacaggac
      601 gcacacacca aatccgtgtg catatgaaat atatcgggca tcctctgccc ggagattatt
      661 tatacaatcc tgattaccgt cggataaacc gccagcccct ccattcttat cagctggagt
      721 ttactcatcc gacaaccgga aaggtcatgc tttttactgc tccgcttccc attgatttca
      781 tttctgcctt ttatagcaat cctctaaaat aatgtattta tagcaattcc ataaattact
      841 gcaaacaaaa cagttcatca gctttttgtc tgatgaactg ttttgatatg aatgcatata
      901 cattcttact tatttttctc ataaatgatc agaagacctc tcagaagaag ttcctcatcc
      961 agaatgattt ctcttttaca atggggaacg attttctttg ccagaccacc ggttgccact
     1021 acagttgcct tctcccccaa ttcctcttcg atcctgtcaa taattccatc cagagctgct
     1081 gcattgctgt aaagaacccc actcttcata cattcgatcg tgtttttccc aatcacatgt
     1141 cttggcgcat caatactgat tccgctgagc tgggaagccc tcgcagtaag tgcatccagg
     1201 gaaaccccta cacccggaag aatcattcct cctacatact gcttcttact gttgacaaca
     1261 gacgctgtgg ttgcagttcc catatcaata aggattaacg gcaccggata accggcaatt
     1321 cctgccactg catcagcaac ctgatctgca ccaagctgtc ccggattatc catcataata
     1381 ttcagtcctg tctttacacc aggtccgaga acaattgcat ttttcttaag aattttctct
     1441 gcggcaagtt tcacaatatt tgtgatctgc ggcactacag aagaaataat acagccctca
     1501 atctctgctt tttttatatg atagatatca agaacattct ttaaatccac cgcatattcc
     1561 agttccgtct ttgtacggtt agtagaaagg cgttctataa aatatgtttt ccggtcatca
     1621 atacatccaa ctacaatgtt ggtgtttcca acgtcaattg ctaaaatcat aattatttat
     1681 ctccaatttc ttaatcgtcc tgttccctta acagacagaa tcctgcatca gcgaaagaat
     1741 tttatccagg atcctaaaag ctgcgtcttc tttactcata agcggaagag agatttcctc
     1801 atcctgtgtg atcaatgtca gtacattggt atctccctga aatccggcgc cctccacttt
     1861 cacattgttg gcagccacca tatccagatt tttctttgcc agcttggctc tggaattgtc
     1921 gatcacattc tgtgtctcca tggaaaatcc gcagagaaac tgtccatgct gtttgtgttc
     1981 cccaagaaac ttcaggatat catctgttct ttccagttct atggacatct gtccatcact
     2041 tttcttaact ttttcttcac tgatcctggc agggcggtaa tctgccacag cagcagcttt
     2101 aataatgatg tcctgttcgt tgctgacaga agttacagct tcatacatgt ctcttgcagt
     2161 cacaacaggt atcagattta caaacatggg aggttcaatt gaagtacgtc cgctgaccag
     2221 cgtgacatct gcaccgcgga gcatggcagc tttcgcaata gcatagccca tttttcctga
     2281 agaatgattg gtaagataac gaacaggatc aacagattcc tgtgtaggac ctgcagttac
     2341 aaggattttc tttcctttca gatctttctc acaggcagct tcgcgctcaa tataggcaag
     2401 taatgtctca ggttccggca tttttcccgc tcctgtgtca ccacatgcca gatatccgca
     2461 cgccggagcg atcacttcat atccgtaatg ctccagaatc tttaaattat cctgtactac
     2521 aggattctca aacatattgg tattcatggc cggggaaatg aattttttac atttgcatgc
     2581 catgatcgtg gtagtaagca tgtcatctgc gatcccatgt gccagtttac cgatcacatt
     2641 agcacttgcc ggtgcgatca tcactacatc tgctttcttt gcaatcgata catgctctac
     2701 ctgaaactga aaattccggt caaaggtatc caccagacat ttatttcctg tgagagtttc
     2761 aaaagtgatc ggattgataa aattcgtggc attctctgtc atcagcacat gaacatctgc
     2821 atggcgtttc ttcagcgcgc ttgccagata tgcaatctta tacgccgcaa tgctgcctgt
     2881 cactcccagt aatactgttt ttccttccag tgtcattttc ctttacctct ttctccgaac
     2941 catattattt ctggctgttt ctacgtcttt atacttacat gtcaaaatct atcataattc
     3001 atctttgata acaggattct gacttttaca attctattta ttatagacta attattatca
     3061 aaaatgcaat aagtgatttg ttttatttgc gaaacaatca gaacgttact gttcacagta
     3121 acgtagccaa aattcattcc agattgcctg cggcaatgga attttggctt gtatgtctcg
     3181 ggattttggc atattcatgc caaaacacct cgcgggatag aggtatgtga acagtaacgg
     3241 tgaacctcag caagattaca cttaaccgtt gtttataaag atttcctgca ttataataat
     3301 aatcagtcag atatccataa tttgcgctgg actctacctg acttaactga cagaccggat
     3361 tactataata tactgatatc aacatataaa tccggtgtat gactttgtgg aggaatatag
     3421 gaatatatga agaattatca gatcacgcaa tggtgcgcag cttttatcag acagcaggta
     3481 caggaaggag acttctgtat tgatgctaca atgggaaacg gaaatgatac gcttctgctt
     3541 tcgcagctat gcggagaatc aggaaaggtt cttgcctttg atattcagga gcaggcactg
     3601 actgcaaccc aaaagagact gaacgccgga catgttccgg aaaattaccg actgcttctg
     3661 gaatctcacg caaatatggc agaatatgcc acaccagaca gtgtaagctg cattgtattt
     3721 aattttggtt atcttcccgg aggagatcat tcacttgcaa ccagaggtaa aaccagcatt
     3781 caggcattga cccaggcttt aacccttctg aaaaaaggcg gaatgatttc tctttgcatc
     3841 tacagtggcg gcgattccgg atttgaagag cgggatcaga tccttgactg gctgaaaaac
     3901 cttgatcccc atcaatatct ggtaatcaaa agcgaatatt ataatcgccc caacaaccca
     3961 ccgataccag tgctgataat taaaaattaa ctgtatctgt tccaaaaagc agcagaaaag
     4021 atttttcata tctctcctgc tgcttcttgt tatgcctttt cttcaatttt tgtttgttct
     4081 ccgggcactt atacttcgat ttctgtttta cttaatctgt aatggtaatc ttctcaataa
     4141 ccggctgctg atctgacggg attgttccat tgtcgtctgt cggttttgca tcctcgcaga
     4201 tcttatcaac aatatccatg ccttctgtca catggccaaa tcctgcataa tctccgtcca
     4261 gaaatgtact gtctgcctga acaataaaga actgcgagct tgcgctgtcc ggatcactgg
     4321 ctcttgccat ggaaatagtc cctcttgtgt gggaaatatc attgtctact ccattgttgc
     4381 tgaactcacc cttaatattc tcttcagatc cacctgttcc gtctccgttg ggatcaccac
     4441 cctggatcat gaatccatcc atgatacggt ggaatgtcag tccgtcataa aaaccttcct
     4501 gtgcaagttt cacgaaattt gtcactgtga tcggtgctgt atctgcatcc
//
LOCUS       DBMDXG010000144         4517 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4617_length_4517_cov_1.877264, whole genome shotgun sequence.
ACCESSION   DBMDXG010000144 DBMDXG010000000
VERSION     DBMDXG010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4617_length_4517_cov_1.877264"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="ACZPD3_05140"
     CDS             <1..306
                     /locus_tag="ACZPD3_05140"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR1415422.1"
                     /translation="IGLINKLRAYELQEQGMDTLEANLALGFAGDEREYYIGAQILRN
                     LGVKELRLLTNNPDKVYQLEEFGIRIKQRVPIQMNATAYDLRYLKTKRLRMGHILKY"
     gene            421..888
                     /gene="ribH"
                     /locus_tag="ACZPD3_05145"
     CDS             421..888
                     /gene="ribH"
                     /locus_tag="ACZPD3_05145"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR1415423.1"
                     /translation="MKTLEGKLVSDGMKVGIVAARFNEFITSKLVSGAMDGLTRHNVK
                     EEDIQVAWVPGAFEIPLIASKMAKSGKYDAVICVGAVIRGNTSHYDYVCNEVSKGIAS
                     VSLETGVPVLFGVLTTENIEQAIERAGTKAGNKGYDCALSAIEMVNLIKEFEA"
     gene            complement(885..1934)
                     /locus_tag="ACZPD3_05150"
     CDS             complement(885..1934)
                     /locus_tag="ACZPD3_05150"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1415424.1"
                     /translation="MERYLEFRIDEQTTELKMEAFLKKNAGLTKRQISQAKFRPDGIT
                     RNGVRCRVTETIYPGDVIRVCLEEAGTASAHLENYNDGIKSKDLSDFHSNKASAPVSE
                     TSFSLNILYEDTDILAVDKPAGMVTHPTGMHYTDSLSNLVASYFREKNEQVCVRPVGR
                     LDQETSGIVVFAQNQVAASRLQSVKSPFKIHKQYLAAVSGTLPVDMPGVWHTLDLPLM
                     QDPENHLKMKTAADLSLHSSALSGKIKTAVTHYHTLFSSQDWSLITLKLDTGRTHQIR
                     VHMASTGHPLLGDTLYHSDDKISSCASFSRAALHAWKVSFQHPFRDEMLLLEAPLPFD
                     FRELVSLSGSDLLSI"
     gene            2111..3022
                     /locus_tag="ACZPD3_05155"
     CDS             2111..3022
                     /locus_tag="ACZPD3_05155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR1415425.1"
                     /translation="MKYLIIGAGGTGGVTGYYMKKAGKDVTLIARGEHLKKIQKQGLT
                     LEKMWDKTEENISIPATDMEHYAEHPDVIFVCVKGYSLQETIPFIKRIARKNTIVIPV
                     LNIYGTGGKMQKELPDLLVTDGCIYVSANIKEPGKLLQHGQILHIVFGVRDKAEFRPE
                     LKEIQKDLCDSHIDGTLSENIRREALEKFSYVSPIGAAGLYYHATAADFQKEGEAREL
                     FKTMIKEIAALAEAMGVPFQKDMAEVNLKILSNLAPEATTSMQRDIMAGKQSEIDGLV
                     YEVVRMGEEYHVPVPAYEKVAEKLRAL"
     gene            complement(3160..>4517)
                     /gene="pckA"
                     /locus_tag="ACZPD3_05160"
     CDS             complement(3160..>4517)
                     /gene="pckA"
                     /locus_tag="ACZPD3_05160"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR1415426.1"
                     /translation="KNDNHPASQEAWDSCKDLALKELSNKRLFVVDAFCGANKDTRMA
                     IRFIVEVAWQAHFVTNMFIQPTAEELENFEPDFVVYNASKAKVENYKELGLNSETAVM
                     FNITTKEQVIVNTWYGGEMKKGMFSMMNYFLPLKGMASMHCSANTDMNGENTAIFFGL
                     SGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFEGGCYAKVINLDKESEPDIYNAIKRN
                     ALLENVTLDAEGKIDFADKSVTENTRVSYPINHIENIVRPISSAPAAKNVIFLSADAF
                     GVLPPVSILTEAQTQYYFLSGFTAKLAGTERGITEPTPTFSACFGQAFLELHPTKYAE
                     ELVKKMEKSGAKAYLVNTGWNGTGKRISIKDTRGIIDAILNGDILNAPTKKIPFFDFE
                     VPTELNGVDTGILDPRDTYADASEWEEKAKDLAGRFIKNFAKYEGNAAGKALVAAGPQ
                     L"
BASE COUNT         1385 a          888 c         1030 g         1214 t
ORIGIN      
        1 atcggactga tcaataaact tcgtgcctat gaacttcagg aacagggaat ggatacactc
       61 gaagcgaatc tggcacttgg atttgccgga gacgaaagag aatactacat tggtgcgcag
      121 attctgagaa atcttggggt aaaagagctg aggctgctga caaataaccc ggataaagtt
      181 tatcagttgg aagaatttgg catcaggatc aaacagagag ttccgataca gatgaatgcc
      241 acagcgtatg atctgcgtta tcttaagaca aaacgattac gaatgggaca tattctgaag
      301 tattaaatac agcggggatt ttcatatata acgataacgt tcccgaaaat aaaatttacc
      361 gtatataaaa acctgtacag gttaaaaaat ataaatttac agatgaaagg accagaaaaa
      421 atgaaaacat tagaaggaaa attagtatca gatggaatga aagtaggaat tgtagcagcc
      481 cgcttcaatg aatttataac ttccaagctg gtaagtggtg ccatggatgg gctgacacgt
      541 cacaatgtaa aagaagagga catccaggta gcgtgggtac caggtgcttt tgaaatccca
      601 ctgatcgcgt ctaaaatggc aaaaagtgga aagtatgatg cggttatctg cgtaggtgct
      661 gtgattcgtg gaaataccag tcattatgat tatgtatgca atgaggtttc caaagggatt
      721 gcatcagtat cactggaaac aggagttccg gtattattcg gagtgcttac cacagaaaac
      781 atcgagcagg caattgaacg cgcaggtaca aaagcaggaa ataaaggcta tgactgtgca
      841 ctcagtgcca ttgaaatggt aaatctgatc aaagaatttg aagcttaaat agacaacaga
      901 tcagaccctg aaagtgaaac cagttcacga aaatcgaaag gtaatggtgc ttccagaagg
      961 agcatttcat ctctgaaagg atgctgaaaa gaaactttcc atgcatgaag ggctgccctg
     1021 gaaaaggaag cacaggaaga tattttatcg tctgaatgat aaagagtatc tccaagcaaa
     1081 ggatgtcctg tggatgccat atggacacgg atctggtggg tacgtccggt atccagctta
     1141 agtgtgataa gcgaccagtc ctgactggaa aaaagagtat gataatgagt aactgctgtt
     1201 ttgatttttc ccgataatgc agatgaatgt aaggaaagat cagcagcagt tttcattttc
     1261 agatgattct ccgggtcctg catcagcggt aaatctaagg tatgccagac accaggcata
     1321 tctacaggca aagtaccgga tacagcggca agatattgct tgtgaatttt gaatggagat
     1381 tttacagatt gaagccttga agctgctacc tgattttggg caaaaaccac aatcccggaa
     1441 gtctcctgat ccagccgccc caccgggcgg acacagacct gttcattttt ctctcgaaag
     1501 taactggcaa caagattgga gaggctgtct gtataatgca ttcccgtcgg atgcgtaacc
     1561 attcctgccg gtttatcaac tgccagaata tccgtatctt catataaaat attcagagaa
     1621 aaagaagttt cggaaactgg agctgaagct ttgttggaat ggaaatccga caaatcttta
     1681 cttttgattc cgtcattata attttcaaga tgcgcagaag cagtcccggc ttcctccaga
     1741 caaacgcgga tcacatcgcc tggatatatt gtctcagtta cacgacagcg aactccgttt
     1801 ctggtaatcc cgtccggtcg aaacttcgcc tggctgatct gccgttttgt aagtcctgca
     1861 ttctttttca gaaatgcttc cattttaagt tctgtagttt gttcatctat tctaaattcc
     1921 agatatcgtt ccatcttaat acttttcctg tgtaatatat tctgatattg ataccattga
     1981 tattacaggg caaaaaataa actgtcaatg caaaattatg tcagcgtaat gcataaaata
     2041 tactgaagaa aaattgcaat tgtgctatga tataagaatc aaaacaatga aagcggggca
     2101 gaaaataaat atgaaatact taatcattgg agccggagga accggtggtg ttaccggata
     2161 ttatatgaaa aaagcaggca aggacgtaac cctgatcgcg agaggcgaac acctgaaaaa
     2221 gatccagaaa cagggtctta cattggagaa aatgtgggat aaaacagagg aaaacataag
     2281 cattcctgct acagatatgg agcattatgc agagcatccg gacgtaattt ttgtctgcgt
     2341 aaaaggctat tccttacagg aaacgattcc gtttatcaaa cgcattgcca ggaaaaatac
     2401 tatcgtaatt ccagtgttga atatatatgg aacaggtgga aaaatgcaga aagaactgcc
     2461 ggatcttctg gtaacggacg gctgcatcta tgtttctgcc aacattaaag aaccgggaaa
     2521 acttctccag catggacaga tcctgcacat tgtattcggc gtaagagaca aagcagaatt
     2581 ccgaccggaa ctgaaagaga ttcagaaaga cctctgtgac agtcatatcg acgggactct
     2641 ttcggaaaac atccgccgtg aagcactgga gaaattttcc tatgtatccc cgatcggggc
     2701 agcaggactc tattaccacg caactgccgc agatttccag aaagaaggcg aagccagaga
     2761 actttttaag accatgataa aagaaatcgc agcacttgca gaagccatgg gagttccttt
     2821 ccagaaagat atggcagaag taaatttaaa aatcctttcc aacctcgcac cggaagcaac
     2881 cacttccatg cagcgggata tcatggcagg aaaacagtcc gaaattgacg gattagttta
     2941 cgaagtagta agaatgggag aagaatatca cgttccggta cccgcatatg agaaagtggc
     3001 agaaaaactc agagccctgt agcgaagcgg attgcgaata tccgcttgac caccgaaaca
     3061 acatgaaaaa gggatgcgaa ttactcgcat ccctttcgta ctcatttgga caataaaatt
     3121 ataatttaat tataattaac tttaattcag caattataat tataactgag gaccagctgc
     3181 aacaagtgct ttaccagcag cgtttccttc gtatttagca aagttcttga tgaatcttcc
     3241 ggcaagatct tttgcttttt cttcccattc agaagcgtca gcatatgtgt cacgaggatc
     3301 aaggatacct gtgtctacgc cgttaagctc tgttggaact tcgaaatcga agaatgggat
     3361 cttcttagta ggagcgttga ggatatctcc gttaaggatt gcatcgatga taccacgagt
     3421 atctttgatg gagatacgtt ttccagttcc gttccatcct gtgttaacga ggtaagcttt
     3481 tgctccgctc ttttccattt tcttaacaag ttcttctgcg tattttgtcg gatgcagttc
     3541 aaggaatgcc tgtccgaagc aagcagagaa tgtaggtgta ggctctgtga ttccacgctc
     3601 tgtaccagca agtttagctg tgaatccaga caggaaatag tactgagtct gagcttctgt
     3661 caggatagat actggaggaa gtactccgaa agcgtctgcg gacaggaaga ttacgttctt
     3721 agctgccggt gcagaagaga tcgggcgaac gatgttttca atgtggttga tcggataaga
     3781 tacacgagta ttctctgtta cgctcttatc tgcgaaatca atcttgcctt cagcatcaag
     3841 tgttacattc tcaagaagag cgttacgttt gatcgcattg tagatgtccg gctcagattc
     3901 tttgtcaagg ttgataactt tagcgtagca tccgccttcg aagttgaata cgccgttgtc
     3961 atcccagcca tgctcgtcat caccgatcag gagacgtttc gggtctgtgg aaagagttgt
     4021 tttacctgtt ccggaaagac cgaagaagat agctgtgttt tcgccgttca tatctgtgtt
     4081 tgcagagcag tgcatggaag ccatgccttt cagtggaagg aagtagttca tcatagagaa
     4141 catacctttc ttcatttctc cgccgtacca tgtgtttacg ataacctgct ctttagttgt
     4201 gatgttgaac attacagctg tttcagagtt aaggcctaat tctttatagt tttcaacttt
     4261 agctttggaa gcgttgtata caacgaaatc tggctcgaag ttttcaagtt cttcagctgt
     4321 tggctggatg aacatatttg ttacgaaatg tgcctgccaa gcaacttcaa cgatgaaacg
     4381 gatagccata cgagtatctt tgttagcacc gcagaatgca tctactacga aaagtctctt
     4441 attggagagt tctttcagag caagatcttt acagctgtcc catgcttcct gggaagccgg
     4501 atggttgtcg tttttgt
//
LOCUS       DBMDXG010000145         4498 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4646_length_4498_cov_1.612918, whole genome shotgun sequence.
ACCESSION   DBMDXG010000145 DBMDXG010000000
VERSION     DBMDXG010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4646_length_4498_cov_1.612918"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            146..763
                     /locus_tag="ACZPD3_05165"
     CDS             146..763
                     /locus_tag="ACZPD3_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR1415427.1"
                     /translation="MTIDDKDMINSILESVSRIDYIKPEDIPNIDLYMDQVTTFMEEQ
                     LVSSKRYADDKILTKTMINNYAKNKLLPPPEKKRYSKEHVLMLIFIYYFKNILSINDI
                     QTLLTPIAQKYFKSMTEKDMTYIYNEVFSMEKEQIESLKKDLLRKYKTAQDTFGDADE
                     EDQANLKRFSFICLLSFDVYVKKMMIEHIIDGMNPEPEHNSKKKK"
     gene            complement(906..3107)
                     /locus_tag="ACZPD3_05170"
     CDS             complement(906..3107)
                     /locus_tag="ACZPD3_05170"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR1415428.1"
                     /translation="MEWIKYHEAEKVFDLRTEHSTYQMQVREYDTLVHLYYGSPVGDA
                     LITDRIVCVDRGFSGNPYEAEKDKTFSLDTLPQEYTAYGNGDYRINGLETEQADGSDT
                     ANLKFESYEITKGKYSLKGLPAMFAKEDEAQTLEIVLTDRASGLKAHLLYGVFPHLDV
                     ITRAVRLENTGTASITVKKAMSVEMDYEYRELDAVHFYGRHNMERQMERTHLGHGNWS
                     VGSIRGTSSHHHNPFVILCDRNTEETYGNCYGYALAYSGNFLFETEVDQVGHTRVAMG
                     IHPYHFSWTLEQGERFETPEVIMAYSAEGFGKLSRIYHDAYRSNLIRSKYVEQPRPIL
                     VNNWEATYFDFDADKIYHIAEEAKNIGLDMFVMDDGWFGKRDNDWCALGDWEVNEEKI
                     KGGLPALAEKIHGLGLKFGLWVEPEMISEDSDLYREHPDWALKIPGRAMNRSRHQLNL
                     DITRKEVREHIMNQIFKVLDACKADYVKWDMNRSVDNVFSAALPKERQGEVYHRYVLA
                     VYEMMESLVQRYPDLLFESCSGGGGRFEAGMLYYSPQIWCSDNTDAIDRLKIQYGTSF
                     GYPISAMGAHVSVCPNHQSGRTTPFETRGIVASAGTFGYELDLMKMSEEEKKTAREQI
                     LKYKEMEHLVQSGDYYRLVNPFENNNHVLWQFVSKDKKETVVCGVRLHSEANPYIYLF
                     YPQGLDADMKYEDTATGKVYTGAALMKAGLPLPLTTGDYQPIRFTFKAVEK"
     gene            complement(3195..3860)
                     /locus_tag="ACZPD3_05175"
     CDS             complement(3195..3860)
                     /locus_tag="ACZPD3_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR1415429.1"
                     /translation="MKPGSLFDRIFGFLGQLIALNLLWIVCSLPVITAGASTTALFYC
                     TLKLHKDGDIRVLHDFFKSFKQNFRQSTLIWILMVAAGIFIYMEKEALATMPGSMSQI
                     FYYVIFAVYIPLVAVALYVFPTVAAFENKTMTLITNAFYFAVKHIGYALAVAVITILP
                     MTMTLVDAKLFPVYLLIWLMFGFSLTAYADSWFMWKLFKPYFKEEEEGHHYVDTEPDQ
                     YAF"
     gene            3941..4054
                     /locus_tag="ACZPD3_05180"
     CDS             3941..4054
                     /locus_tag="ACZPD3_05180"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415430.1"
                     /translation="MPANCDAHLGESIFQTHSKYYQIQFQASPATISGLIL"
     gene            complement(4084..>4498)
                     /locus_tag="ACZPD3_05185"
     CDS             complement(4084..>4498)
                     /locus_tag="ACZPD3_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415431.1"
                     /translation="LPLPDLSEVPYKPAFGTFLLKQAYMGLPKDLEEAARLDGCNIGQ
                     TFLFIMAPLTRSSMVALGIFTAVFAYKDLMWPLICNKDVMPLSAALAKMQGQYTNNYP
                     QLMAASLLACIPMIVLYLIFQKQFIEGIATSGGKL"
BASE COUNT         1242 a         1012 c          854 g         1390 t
ORIGIN      
        1 tttagtattg actttttatt tgcaggctaa agtaacgaga atgcgacgcg cagttattca
       61 agcggatata aattctgtaa tgagtaaaaa aaacatttac aagtagtatt taaaactata
      121 taataaggaa cagaggtgat aaaagatgac aatcgatgat aaagacatga taaacagcat
      181 tctggaaagt gtatccagga ttgactatat taaaccggaa gacatcccaa atattgattt
      241 atatatggac caggtgacaa cctttatgga agagcagctc gtttcttcca aacgttacgc
      301 agatgataag attctcacca agactatgat caacaattat gcaaagaata aacttctccc
      361 cccgcctgag aagaaacgtt attccaaaga acatgtgctg atgctgattt ttatttacta
      421 tttcaagaat attctttcca tcaatgatat ccagactctg ctcacaccga ttgcgcagaa
      481 atattttaaa tccatgactg agaaagatat gacttatatt tataatgaag ttttcagcat
      541 ggaaaaggaa cagattgaat ccctgaaaaa agacctgctg cgcaaataca aaacagcaca
      601 ggatactttc ggggacgcag atgaagaaga tcaggcaaat ttgaagagat tttcatttat
      661 ctgcctgtta agctttgatg tctatgtaaa gaaaatgatg attgagcata taatcgacgg
      721 gatgaatccg gaaccagagc ataattccaa aaagaaaaaa taatatttca aaaagcagca
      781 gatttttcct gatttgggaa aatctgctgc ttttttatgt tatataaaat aatgattatg
      841 ctattttttc ctctgctgtc agagccgatt ccttatcgac tgtgttgaca ccgataatag
      901 ctgtattatt tttccactgc cttaaatgta aatctgattg gctggtagtc acctgttgtc
      961 agtggcagag gcagacctgc cttcataaga gctgcacctg tatatacttt tcctgttgct
     1021 gtgtcttcgt atttcatatc agcatccaat ccctgtgggt agaaaagata aatataagga
     1081 tttgcttcgc tgtggagtct tactccgcac acaactgttt cttttttgtc tttggataca
     1141 aactgccaaa gtacatggtt gttgttttcg aatgggttca ccagacgata gtagtctccg
     1201 gactgtacca gatgctccat ttctttatac ttcagaatct gctctctggc tgtctttttc
     1261 tcttcttcgg acatcttcat cagatccagc tcatagccaa aggtacctgc ggatgcaaca
     1321 attcctcttg tctcaaaagg agtcgttctt ccggactgat ggttcgggca tacgctgaca
     1381 tgtgcgccca ttgcacttat gggatatccg aaagaggtac catactggat tttcagtctg
     1441 tcaatagcat ctgtattgtc actgcaccag atctgcggag aatagtaaag cattcccgcc
     1501 tcaaatcttc cgcctccgcc tgagcaggac tcaaacagaa gatccggata tctctgtacc
     1561 aggctttcca tcatttcata aacagccagc acatatctgt ggtaaacctc tccctgacgt
     1621 tcctttggaa gagctgctga gaatacgtta tctacgcttc ggttcatatc ccattttaca
     1681 taatcagcct tgcatgcatc cagaacctta aagatctggt tcataatgtg ttcgcgtact
     1741 tcttttctgg taatatccag atttaactga tgtctgcttc ggttcatggc acgtcccggg
     1801 atcttcagtg cccagtctgg atgttctctg tacagatcac tgtcctcaga gatcatctcc
     1861 ggctctaccc acagaccgaa tttcagtcca agtccatgga tcttctctgc aagtgcggga
     1921 agtccgcctt taatcttctc ttcatttact tcccagtcgc caagtgcaca ccagtcattg
     1981 tctctctttc caaaccatcc gtcatccatg acaaacatat ccagtccgat attttttgcc
     2041 tcttctgcaa tgtggtaaat cttatctgcg tcaaaatcaa aataagttgc ttcccagtta
     2101 tttaccagga ttggtcttgg ctgctctaca tatttgctgc ggatcagatt gcttctgtat
     2161 gcatcatgat agatcctgga aagctttccg aaaccttctg ctgaatatgc catgatcact
     2221 tccggtgtct caaatctctc tccctgttcc agcgtccagg agaaatgata gggatggatt
     2281 cccattgcta cacgggtatg tcctacctgg tccacctctg tttcaaataa gaagtttcca
     2341 ctgtatgcaa gtgcatatcc atagcagttt ccataagttt cctctgtatt tctgtcacag
     2401 aggattacaa atggattgtg atggtggctg gaggttccgc ggatacttcc cacagaccag
     2461 ttgccatgac caaggtgagt tctctccatc tgacgttcca tattgtgtct gccatagaag
     2521 tggactgcat ccagttctct gtattcatag tccatttcca cagacattgc ttttttcact
     2581 gtaatcgatg ctgtacctgt attctccagg cgtaccgctc tggtgatcac atcaagatgt
     2641 ggaaatacac catagagaag atgtgctttc agtccgctgg cacgatctgt cagaacgatt
     2701 tccagcgtct gtgcctcatc ttcctttgca aacattgcag gaagcccctt caggctgtac
     2761 tttccttttg tgatctcata ggactcaaat ttcagatttg cagtatcact gccgtctgcc
     2821 tgctcagtct ccagaccatt gatacgataa tcaccatttc catatgcagt atattcctgt
     2881 ggcagggtat ctaatgaaaa tgttttgtcc ttttctgctt cataaggatt tcctgaaaat
     2941 cctctgtcca cacatacgat cctgtctgtg atcagtgcat cgcctaccgg acttccataa
     3001 tacagatgaa ccagagtatc atactctctt acctgcatct gataagtgct gtgctctgtt
     3061 ctcaggtcga atactttctc tgcctcgtga tatttgatcc attccatact ctgtacctct
     3121 catttctttg ttctttctat tttatagatg ttctatctat tttttatgta accaggtatc
     3181 gctgtttgtt tcggttaaaa cgcgtactga tcaggttctg tgtccacgta gtgatgtccc
     3241 tcttcctcct ctttgaagta gggcttgaac agtttccaca taaaccagga atctgcatag
     3301 gcagtcaggg agaatccaaa catcagccag atcagcagat ataccgggaa aagttttgca
     3361 tctacaagtg tcattgtcat tggcaggatt gtaatcactg ccacagctaa ggcataacct
     3421 atatgtttaa ccgcaaaata aaatgcgttt gtgatcaaag tcattgtttt attttcaaat
     3481 gctgcaactg ttggaaatac atacagtgca actgccacca gaggaatata taccgcaaag
     3541 atcacataat aaaaaatctg tgacatggat cccggcattg ttgccagagc ttctttttcc
     3601 atataaataa atattcctgc tgcaaccatc agaatccaga tcagtgtgga ctgcctgaaa
     3661 ttctgcttaa aactcttaaa aaaatcatga agtactctga tatctccatc tttatgaagc
     3721 ttcaatgtac agtaaaataa tgcagtggtg gatgcccccg ccgtgataac cggaagagag
     3781 cacacgatcc atagcaaatt taatgcaatc aactgaccta aaaatccaaa tattctgtca
     3841 aagagacttc ctggtttcat ttctatctac ctcttccttt catttatctt tttttatttt
     3901 atatttcagt tttctttctc tagagcatgt ttaaaaaacc attcccgcaa attgtgacgc
     3961 acatcttggg gaaagcattt ttcagacaca ctctaaatat tatcaaatcc agtttcaggc
     4021 aagccctgct acaatctctg gtttgatttt ataacaaagc ctacctgtgc tgctcagatt
     4081 ttattacagc ttaccaccgg atgttgcaat accttcaatg aactgtttct ggaagatcag
     4141 atacagtacg atcatcggga tacatgcaag cagagatgct gccatcagct gcggataatt
     4201 gttggtgtac tgtccctgca tttttgcaag tgctgctgac agaggcatta catccttgtt
     4261 acagatcaaa ggccacatca ggtctttgta tgcaaatact gctgtgaaga ttcccagtgc
     4321 caccatggaa gaacgtgtca gaggagccat gatgaacagg aaggtctgac cgatgttaca
     4381 tccgtcaaga cgtgctgcct cttccagatc tttcggaagt cccatataag cctgtttgag
     4441 gagaaatgta ccaaatgcag gcttatatgg gacttccgaa agatctggaa gaggcaag
//
LOCUS       DBMDXG010000146         4436 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4712_length_4436_cov_1.417762, whole genome shotgun sequence.
ACCESSION   DBMDXG010000146 DBMDXG010000000
VERSION     DBMDXG010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4712_length_4436_cov_1.417762"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="ACZPD3_05190"
     CDS             <1..895
                     /locus_tag="ACZPD3_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415432.1"
                     /translation="MIQNITGVVFIIFHIICCVLVWTGINSHLLKVKRYLILPVIFVP
                     VWGMICVLLLHFQIFFHQDKKREIGIEKMKINEEIYRSIIAPKEESDRNIVPLEEVLL
                     IDEAAMRRDLIMNVLNDDPENYIDMLKQARMNDDVEVVHYAITGMVELSKEYESRLQK
                     IEYRYAKEPENQQLISEYCDFLQEYLSQGLLEGQMELVQRNQYIKLLKKKLKFKEDLH
                     TYVCLTENQMQMKEYEQVLKSLERMDKKWHRNEEYWILRIRYYVELKQGKELKETLEQ
                     IQQEHIYLSAKGKEALAFWQE"
     gene            892..2721
                     /locus_tag="ACZPD3_05195"
     CDS             892..2721
                     /locus_tag="ACZPD3_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HIR1415433.1"
                     /translation="MIKYLQSFRFKGLTVILGVFLAISVVLWTERSGIQYQAEIKKNA
                     YLDRDTVVTETQAVKNIESSCLVLMDSSQADSVVAWEEFERIFMDMKTGVDLADIRQS
                     EIPDFSGYETIVVLMSDLNPLKDVVIKIGNWVEKGGRVLFALTLQKDTYVSLIEQKLG
                     ITDSDYGNVLVDKIYIDDDFMIGGGRSFQIPDAYDSAWEVSVGETAKVYAWTDDEKKV
                     PLIWENSYGKGKFVVDNFGLCEKATRGFFAASYSLLTDVMVYPVLNGSVFYLDDFPSP
                     VPSGDGTYIKRDYGLSIKEFYTNIWWPDMLELAEEHGVKYTGVIIDNYEDDVSGDVVE
                     QEDVQRFQYFGNMLLHQGGELGYHGYNHQPLSLSNVDYANILPYKTWESYDAMKKAMT
                     ELIRFGKEMFPGTELSVYVPPSNVLSDEGREMIVKEFPEIRTIESNYFVGDMAYTQEF
                     EAAEDGIVEQPRIISGAVIDDYMELAAVSELNMHFVNTHFMHPDDLLDEDRGARLGWE
                     KLKKRLDEYMDWLYTSAPCLRNLTASELSGAIQRYGALVIDKDISDQELNLKLDNFYD
                     EAYIMIRMNEGTPGNIEGGELTHITGNLYLLRAKEKSVKIEIR"
     gene            2718..4169
                     /gene="pelF"
                     /locus_tag="ACZPD3_05200"
     CDS             2718..4169
                     /gene="pelF"
                     /locus_tag="ACZPD3_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HIR1415434.1"
                     /translation="MRKLRICLVLEGCYPYVHGGVSTWMHQYITVMKEHEFVLWVIGA
                     HACDRGKFVYELPDNVVEVHEVFLDDALKLKEHGNQKGQLHRINRFSEEETKSLRELM
                     ECSHPDWEVLFHLYHDRKMNPMSFLKSEQFLNILTESCLEKYPYIAFADAFHTMRSML
                     LPVLYLLGSEVPLADTYHAISTGYGGLLACLGGYVYRRPVLLTEHGIYTREREEEIIR
                     AKWVIPSFKKQWISFFYMLSEAIYKRAFRVTSLFTNAMLTQIQIGCDAEKCRVIENGI
                     NYDRLSQIPLKEEDGWVDIGAVVRLAPIKDIKTMIYAFYELSSRMENVRLHILGGVDD
                     EEYADECYALVKQLDIKNLVFTGRVDIVQYMRKLDFTILTSISEGQPLSVLESFAARR
                     PCVTTDVGCCRELLNGKEGDDFGCAGYCVPPMYRDGLAAAMEKMCESRRRRIRMGKSG
                     QARTKAYYRHERMISEYRKLYREVEEAYGRDWI"
     gene            4150..>4436
                     /gene="pelG"
                     /locus_tag="ACZPD3_05205"
     CDS             4150..>4436
                     /gene="pelG"
                     /locus_tag="ACZPD3_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HIR1415435.1"
                     /translation="MAGIGFELKKLFSRRGLFASFRAYGYAGIICTGPMLLGIVLLLG
                     VMFLCDRTGAPKQSRELLVCMITYTLLASLTVTSFLSMVVTRFIADMLYEE"
BASE COUNT         1413 a          642 c         1143 g         1238 t
ORIGIN      
        1 aatgatacag aatataacag gggttgtttt tattatattc catattatct gttgtgtttt
       61 agtatggaca ggaataaact cccatttact gaaggtgaag aggtatctca tattacctgt
      121 gatctttgta ccggtatggg gtatgatctg tgtattgctt ctgcatttcc agatattttt
      181 tcaccaggat aaaaagcgtg aaatcggaat agaaaaaatg aagatcaatg aagaaattta
      241 cagaagcatt atagcaccga aagaagaaag tgacagaaat attgtgccac tggaggaagt
      301 gcttcttatt gatgaggccg ctatgaggcg tgatctgatt atgaatgtat tgaatgatga
      361 tccggagaat tacatcgata tgcttaaaca ggcaaggatg aatgacgatg tagaggttgt
      421 gcattatgcg atcacaggaa tggtagagct gtctaaggaa tatgaaagca gactgcagaa
      481 gatagaatac agatatgcaa aagaaccgga aaatcaacag cttatttcag agtactgtga
      541 ttttcttcag gagtatttat ctcagggact tctggaagga cagatggaac tggtgcaaag
      601 aaatcaatat ataaaacttc tgaagaaaaa actgaaattt aaagaagatc ttcatacata
      661 tgtatgtctg actgagaatc aaatgcagat gaaggagtat gaacaggttc ttaaaagtct
      721 ggaaagaatg gataagaagt ggcacagaaa tgaagaatat tggatactca ggatcagata
      781 ttacgtggaa ctgaaacagg gaaaagaatt aaaggaaaca ttagagcaga ttcagcagga
      841 acatatttac ctgtcggcga aaggaaagga ggcattggct ttttggcagg aatgataaaa
      901 tatttacagt cttttcgatt taagggtctg actgttattc tgggagtttt tctggcaatc
      961 agtgtggtgc tttggacaga gcgttcagga attcagtatc aggcagagat caagaagaat
     1021 gcatatctgg acagggatac ggttgtaact gaaacacagg cagtcaaaaa tattgagagc
     1081 agttgtctgg tgctgatgga cagcagtcag gcagacagtg tggtggcatg ggaagagttt
     1141 gaaagaattt ttatggatat gaaaacagga gtagatctgg cagatatcag acagagtgag
     1201 attcctgatt tctcaggcta tgaaacaata gtggtgctga tgtcggatct gaacccattg
     1261 aaggatgtag ttataaagat tggaaactgg gtagaaaaag gaggacgtgt tctttttgcg
     1321 ttaacacttc agaaggatac atatgtttcg ctcattgagc agaaattggg aattacagac
     1381 tctgattatg gaaatgttct ggtagataaa atctatattg atgatgattt catgattgga
     1441 gggggaagat ctttccagat tccggatgca tatgattcag catgggaagt aagtgttgga
     1501 gaaacggcga aggtttatgc atggacagat gatgaaaaga aggttccgct tatctgggaa
     1561 aacagttatg gaaaaggtaa atttgtagtt gataattttg gattatgtga aaaagccaca
     1621 agaggatttt ttgcggcatc ctatagtctg ctgacagatg tgatggttta cccggtgctt
     1681 aatggatctg tattctatct ggatgatttt ccatctcctg tgccgagtgg agacggaaca
     1741 tatatcaaac gtgattatgg tctcagcatc aaagagtttt atacaaatat ctggtggccg
     1801 gatatgctgg agctggcaga ggaacacggt gtgaaatata caggcgttat tattgacaat
     1861 tatgaggatg atgtgtccgg agatgttgtg gaacaggaag atgtacagag atttcagtat
     1921 tttgggaata tgctgcttca tcagggagga gaactgggat atcatggata taatcatcag
     1981 ccattgagcc tgtcaaatgt ggattatgca aatatccttc catataaaac atgggaaagc
     2041 tatgatgcaa tgaaaaaagc catgacagag ctgatacggt ttggaaaaga gatgtttccg
     2101 gggacagaat tgtctgttta tgtaccgccg tctaatgtgc tgtcagatga agggcgggaa
     2161 atgatcgtaa aagaatttcc ggagatacgt acaattgaaa gtaattattt tgtcggagat
     2221 atggcatata cacaggagtt tgaggctgca gaagatggaa ttgtggaaca gccccgtatt
     2281 atttcaggtg ctgtgattga tgattatatg gaacttgcgg ctgtatctga actgaatatg
     2341 cattttgtca atacacattt tatgcatccg gatgatctgc tggacgaaga cagaggtgcc
     2401 agactgggat gggagaaact gaaaaaacgg ctggatgaat atatggactg gctgtatact
     2461 tcagctccat gtctgagaaa tcttactgct tccgaactat caggcgcaat acagcggtat
     2521 ggggcactgg tgatcgataa ggatatatca gatcaggaac ttaatcttaa attagataat
     2581 ttttatgatg aagcttatat tatgataaga atgaatgaag ggactccggg taatatagaa
     2641 ggtggagaac tgacgcatat taccggaaat ctctatctgc ttcgggcaaa agagaaatca
     2701 gtgaaaatag aaataaggtg agaaaattga gaatctgtct ggttttagaa ggctgttatc
     2761 cgtatgtgca tggtggagtt tcgacatgga tgcatcagta tatcactgtt atgaaagagc
     2821 atgagtttgt actgtgggtg ataggagcac atgcatgtga ccgtggaaaa tttgtctatg
     2881 aactgccgga taatgtggtg gaagtccatg aggtatttct ggatgatgca ttaaaactga
     2941 aggaacatgg aaatcaaaaa ggacagctgc acagaatcaa tcgtttttcg gaagaagaaa
     3001 caaagagtct aagagaattg atggaatgca gtcatcctga ctgggaagta ttgtttcatt
     3061 tatatcacga ccggaaaatg aatccgatgt catttctgaa aagtgagcag tttttaaata
     3121 tattaacaga gagctgtctg gaaaaatatc cgtatattgc atttgcggat gcgtttcata
     3181 caatgcgttc catgctgctt ccggttttat atcttctggg ttcagaggtt cctctggcag
     3241 atacctatca tgccatttca acaggatatg ggggacttct tgcctgcctt ggaggatatg
     3301 tttacaggag accggtatta cttacagagc atgggatata cacaagagaa agagaggaag
     3361 agatcatcag ggcaaaatgg gtaatcccat catttaaaaa acagtggatt tctttctttt
     3421 atatgttatc agaagcaatt tataaaaggg cttttcgggt aaccagttta tttacgaatg
     3481 cgatgcttac gcaaatacag attggctgtg atgcagagaa atgcagagta attgaaaatg
     3541 gtattaatta tgacaggttg tcacagattc ctctgaaaga ggaagatgga tgggtagaca
     3601 taggtgcggt agtccgtctc gcacccatta aagatataaa gactatgata tatgcatttt
     3661 atgaactctc atccagaatg gagaatgtga gacttcatat tctgggaggg gtagatgacg
     3721 aggaatatgc agatgagtgt tatgcactgg tgaaacagct ggatatcaaa aatcttgttt
     3781 ttacaggaag ggtagatatt gtacagtata tgagaaaact ggattttacg atccttacca
     3841 gtatatcaga aggacagcca ttatccgtgc tggagtcatt tgcagccaga cgcccatgtg
     3901 taaccactga tgtaggatgt tgccgggaac ttctgaatgg gaaagaagga gatgattttg
     3961 gatgtgccgg ttattgcgtt ccgcctatgt acagggatgg acttgctgcc gctatggaaa
     4021 aaatgtgtga atccaggaga agacggatca gaatgggaaa aagcggacag gcaagaacta
     4081 aggcatatta caggcatgag cgcatgatca gtgaatacag aaaactttac agggaggtgg
     4141 aggaagcata tggcagggat tggatttgaa ctcaaaaaac tgttcagcag aagagggctg
     4201 tttgcttctt tccgcgccta tggatatgca gggattatct gtaccggtcc catgttgctt
     4261 ggtatcgtac ttctgctggg ggttatgttt ttgtgtgaca ggacaggcgc tccgaaacag
     4321 agcagagaat tactggtctg catgatcaca tatacacttc ttgcatcatt gacggtaacc
     4381 agtttcttgt caatggtagt aacaagattt attgcagata tgctttatga agaaaa
//
LOCUS       DBMDXG010000147         4429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4724_length_4429_cov_1.688771, whole genome shotgun sequence.
ACCESSION   DBMDXG010000147 DBMDXG010000000
VERSION     DBMDXG010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_4429_cov_1.688771"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            145..>482
                     /locus_tag="ACZPD3_05210"
     CDS             145..>482
                     /locus_tag="ACZPD3_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1415436.1"
                     /translation="MEQRYTVTQTAEILGVRASVLRYWEEELELRICRNEQGHRYYTG
                     NDITLLANIKELKKRGLALRAIKELVPRISRTAPGTTASKVKLLEDEQPEREKILEFQ
                     KIMERLIAQEL"
     assembly_gap    483..492
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(648..821)
                     /locus_tag="ACZPD3_05215"
     CDS             complement(648..821)
                     /locus_tag="ACZPD3_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR1415437.1"
                     /translation="MAYVISDECVSCGTCESECPAEAISQGDEHYVIDADACLDCGTC
                     ADACPTEAIHPAE"
     gene            1290..2420
                     /locus_tag="ACZPD3_05220"
     CDS             1290..2420
                     /locus_tag="ACZPD3_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1415438.1"
                     /translation="MSAPVFAEDEGKGIAKEDLKVGVIYIGDENEGYTAAHMKGIDEM
                     EEKLGLDDSQIIEKTLIGEDEGCYDAAADLADQGCQIIFANSFGHETYILEAAGEYPE
                     VQFCHATGTQAASSGLSNMHNYFTNIYEARYVSGVVAGLKLNEMIEDGTVKEDACKMG
                     YVGAFPYAEVISGYTAFYLGAKSVCPSVTMEVKYTNSWASFDLEKECADALISDGCVL
                     ISQHADTTGAPTACEAAGVPCVGYNIDMTSVAPNTALTSASMDWGVYYTYAVQCMLDG
                     TAIDTDWCKGFAEGADKITALNDKTVAEGTEEKVKEVEDALIDGSLHVFDTSAFTVDG
                     KELDTYKKGDTEYISDGYFHESEYGSAPAFDIAIDGITSITE"
     gene            2675..4210
                     /locus_tag="ACZPD3_05225"
     CDS             2675..4210
                     /locus_tag="ACZPD3_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415439.1"
                     /translation="MGENYALELRDISKSFGSVQANDHVDLTLRKSEILAILGENGSG
                     KTTLMNMIYGIYYPDEGHIFVNGKEVTIRSPKDSYELGIGMVHQHFKLVDVLTAAENI
                     VLGLPGKGKLDMKRITEDIQKLADKYGFELDLSQKIYEMSVSQKQTVEIIKMLYRGAR
                     ILILDEPTAVLTPQESDRLFDILRNMRKDGCSIMIITHKLQEVLALSDRVAILRKGKY
                     IDTVETAQANAQSLSEMMVGGRVDLNIDRPEPENVRKRLVVKGLNCKNKEEVKTLDDV
                     DLTVNAGEILGIAGISGSGQKEFLEAIAGLQAIESGSITLLDDNGKGTELAGMDSISI
                     NKAGISLAFVPEDRIGMGLVGDMDMTDNMMLRSYRNGKSPFLDRKGPKALALKIKEQL
                     EVMTPSISAPVRQMSGGNVQKVLVGREIAQNPKVLLVAFPTRGVDVNTSHVIYRLLNE
                     QKKKGVAVVCVIEDLDVVLELCDRIAVFCGGKISGIEDGRTATKEEIGLLMTKHEKGG
                     TQA"
     gene            4207..>4429
                     /locus_tag="ACZPD3_05230"
     CDS             4207..>4429
                     /locus_tag="ACZPD3_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415440.1"
                     /translation="MKKEPFLQVSKRRNKARWQERLLIRFIALILALIVCGAVIVALV
                     KMNPVDVYKAIWDGAMGSERRLWQTIRDTM"
BASE COUNT         1415 a          765 c         1126 g         1113 t
ORIGIN      
        1 tttataataa atatacatga tatcaggatt acgacagctg tttttacagt gcagtggttc
       61 aatgatatca ggtgatagat tgagttgttg atatgcatca gtggtttctg atgaaaaaat
      121 ggactcaaaa ggaggagggg cagcatggag cagcggtata ctgtgactca gaccgcagaa
      181 atccttggcg tgagggcatc ggtcctccgg tactgggagg aagaactgga actgaggata
      241 tgcaggaatg agcagggaca tcgttactat acaggaaatg atattacgct tctggctaat
      301 ataaaggaac taaagaaaag aggacttgcc ctgagagcga tcaaagaact ggttccaaga
      361 atttcaagga ctgcacccgg gactactgcg agcaaagtga agcttcttga agatgaacag
      421 cctgagaggg aaaaaatact tgaatttcag aaaatcatgg aacgtctgat cgctcaggag
      481 ctnnnnnnnn nncgatccgt aacagacagc ttgcgagaaa agaggctgct gcagcactgg
      541 aaaaaagaag caggaggatc agaagaaaac atttgtaaaa gttatccata aagaaaacag
      601 cttcgtctct gcggtaaaca gagatgaagc tgttttgaaa tcatgaatta ttctgctgga
      661 tgaatagcct ctgttggaca tgcatctgca catgttccgc agtctaagca tgcatcagcg
      721 tcgattacat aatgctcatc tccctgagag atagcttctg caggacattc gctttcgcat
      781 gttccacagc ttacacattc gtctgaaatt acatatgcca taattagaat cctccttatc
      841 gtaataacgt tttaggcccc tttcggggtt ctgcatacat tttaatatat ctttgtctga
      901 attacaagta taaaatcaga tacattataa aataatatta agttagcatt tgcttacttt
      961 acctgcagat tacaaatagc tgacggacat gtgaatgtct gtccgagcag agaaattatc
     1021 ctgccaaaat acaggcattt gtatgatgga aaatagtctg ttaatataca aaaaaaataa
     1081 gagtatcaaa aagaaagtgc gtttttgctg aatttattga caattcagac ataaacggtt
     1141 atagtaaaga tacttgcaag gaggcataac acagaaagtt gtgcctcttt tgttccgtag
     1201 caggaatcag aaaacaaata gaaagaatgg aggaatgaag aatgaagaaa gcattggcaa
     1261 ttggacttgc agcagtaatg gcggtttcca tgagtgcacc ggtatttgca gaagatgaag
     1321 gtaaagggat tgcaaaagag gacctgaaag taggagtgat ctatatcggt gatgagaatg
     1381 aaggttacac ggctgctcat atgaaaggaa ttgatgagat ggaagagaag cttggtcttg
     1441 acgattctca gattattgag aaaactctca tcggtgaaga tgaaggatgc tatgatgcgg
     1501 cagcagacct tgcagatcag ggatgtcaga tcatctttgc aaacagcttt ggacatgaga
     1561 cttatatact ggaagcagca ggggaatatc cggaggttca gttctgtcat gcaacaggaa
     1621 cacaggctgc atccagcgga ctttccaaca tgcataacta ttttacaaat atttatgaag
     1681 cacgttacgt gtcaggtgta gttgcaggtc tgaaactgaa tgaaatgatc gaggacggca
     1741 cagtaaaaga agacgcatgc aagatgggtt atgtaggtgc attcccatac gcagaggtta
     1801 tctccggtta tacagctttc tatcttggcg cgaagagcgt atgcccgtct gtaacaatgg
     1861 aagtaaaata tacaaacagc tgggcaagct ttgatcttga gaaagagtgc gcagatgctc
     1921 tgatctctga cggctgtgta ctgatcagcc agcatgcaga tacaacaggt gctcctacag
     1981 catgcgaagc agcaggagtt ccatgtgtag gttataatat tgatatgaca tctgttgctc
     2041 cgaacacagc acttacttct gcatctatgg actggggtgt atattataca tacgcagtac
     2101 agtgtatgct tgacggcaca gcaattgata ctgactggtg taaaggattt gcggaaggtg
     2161 cagataagat cacagctctc aatgacaaaa ctgtagcaga gggtacagaa gaaaaggtca
     2221 aagaagtgga agatgcgctc attgatggtt cccttcatgt atttgataca tctgcattta
     2281 cagtagacgg caaggaactt gatacttata agaagggtga tacagagtac atttctgacg
     2341 gatacttcca tgaatctgaa tatggttccg cacctgcatt tgatattgcg attgatggaa
     2401 ttacatctat tacagaataa gaaaattgct ggagctgcaa agcgcagagc aatttacgac
     2461 agcagggctg cccgggcggc agctctgctg ttttcatatc gtacgtgcaa aagaagtctt
     2521 gaagtcttct gacagaaggt ttcttttgct gtattatgga gtatacagga gagcaattcc
     2581 ataattcaga agtaggatta aatgatatcg gcagtcaaag agacaggact tttactgtag
     2641 attggatatc attagataaa aggaggaaac gatagtggga gaaaactatg cacttgaact
     2701 tagagatatt tccaagagtt ttggttcggt ccaggcgaat gatcatgtag atctcacact
     2761 gagaaaatcg gagattcttg caattctggg ggaaaacgga agcggaaaga caactctgat
     2821 gaatatgatc tatggaattt attatccgga tgaaggacat atttttgtaa atggaaaaga
     2881 agtaacgatc cgttcaccga aagattccta tgaactggga atcggtatgg tacatcagca
     2941 ttttaaactg gtagatgtac tgacagcagc agagaatatt gttctgggac ttccgggaaa
     3001 gggaaagctg gacatgaaga ggatcacaga ggatattcag aagctggcag ataaatatgg
     3061 ctttgaattg gatctgtcac agaagatcta cgaaatgtcc gtttcccaga agcagacagt
     3121 tgagatcata aagatgctct accgtggcgc gaggattctg atcctggatg agccgactgc
     3181 agttctgaca ccacaggagt ctgacaggtt attcgatatt ttgagaaata tgagaaaaga
     3241 tggatgttcc attatgatca tcacgcacaa actccaggaa gttctggcgt tatcagatcg
     3301 tgtggcaatc ctcagaaaag gaaagtatat tgatacagta gaaacagctc aggcaaatgc
     3361 acagagtctg tcagagatga tggtaggcgg cagagtagat ctcaatatcg accgtcctga
     3421 accggagaat gtaaggaaac gattagtagt caaaggtctg aactgtaaaa acaaagaaga
     3481 ggtaaaaact ctggatgatg ttgatctgac agtaaatgca ggagagattc tgggtattgc
     3541 cggcatttcc ggaagtggcc agaaagaatt tctggaggca attgcaggac tccaggcaat
     3601 agagagcggt tcgatcaccc tgcttgatga caatggaaaa ggaacagagc ttgcaggaat
     3661 ggattctatc tccattaata aagcagggat cagccttgct tttgttccgg aagaccgtat
     3721 tgggatgggg cttgtgggcg atatggatat gactgataat atgatgctca gaagctatcg
     3781 caatggaaaa agtccattcc tggatcgaaa aggtccaaag gcactggcat taaaaattaa
     3841 agaacagctt gaagtcatga ctccaagtat ttccgcacct gtccgtcaga tgtcaggagg
     3901 aaatgtacag aaggttctgg taggaagaga aatcgcccag aatccgaaag tgcttctggt
     3961 agctttcccg actcgagggg tggatgtcaa tacctcccat gtgatctacc gtcttctgaa
     4021 tgaacagaag aaaaaagggg ttgcagtagt ctgtgttatt gaggatcttg atgtagttct
     4081 tgaactgtgt gacaggattg cagtgttctg cggaggtaaa atcagcggaa ttgaggatgg
     4141 aagaactgca acgaaagaag aaatcgggct tcttatgacg aagcatgaaa aaggagggac
     4201 acaggcatga agaaagaacc tttcttacag gtaagtaaaa gaagaaacaa agccagatgg
     4261 caggaacgtc ttctgatccg tttcattgca ttgatccttg cactgatcgt gtgcggagcg
     4321 gtgatcgttg ccctggtaaa gatgaatccg gtggatgtct ataaggcaat ctgggatgga
     4381 gcaatgggtt cggaacgccg tctgtggcag accatacgag atacaatgg
//
LOCUS       DBMDXG010000148         4421 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4736_length_4421_cov_1.505556, whole genome shotgun sequence.
ACCESSION   DBMDXG010000148 DBMDXG010000000
VERSION     DBMDXG010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4736_length_4421_cov_1.505556"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="ACZPD3_05235"
     CDS             <1..615
                     /locus_tag="ACZPD3_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dehydroquinate synthase/iron-containing alcohol
                     dehydrogenase family protein"
                     /protein_id="HIR1415441.1"
                     /translation="VKSSIQDITEGEEDECEQLMYALILSGLAMQMIGNSRPASCAEH
                     QVTHLWDMEVINGPLDALHGEKVSVAALLVLEEYKRIAAAITQGRCHAKPYENEDEEL
                     LKETFGKKGLLEEIRKENEPELLETISPQHLEKCLNGIEEIIDELPSEQTMFHLLEKA
                     GCAKTVYDIGLDEAAVLPSLRLAPYTRRRLSLLRISKMLDIRGE"
     gene            618..1100
                     /gene="ispF"
                     /locus_tag="ACZPD3_05240"
     CDS             618..1100
                     /gene="ispF"
                     /locus_tag="ACZPD3_05240"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR1415442.1"
                     /translation="MRVGMGYDVHRLTEDRDLILGGVKIDWEKGLLGHSDADVLIHAV
                     MDALLGAAALGDIGKHFPDTDPAYKGISSILLLEHVTKLLREHHYEIGNIDATIIAQK
                     PKMAPHIPQMRANMAKAMGINESQLNIKATTEEKLGFTGREEGIASQAICLLNERKES
                     "
     gene            1128..2537
                     /gene="cysS"
                     /locus_tag="ACZPD3_05245"
     CDS             1128..2537
                     /gene="cysS"
                     /locus_tag="ACZPD3_05245"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR1415443.1"
                     /translation="MKLFNTLTRRKEEFVPLEEGKVRMYVCGPTVYNLIHIGNARPMI
                     IFDTVRRYMEYKGYEVNYVSNFTDVDDKIIKKAIEEGVSAQEISERYIAECKKDMDGM
                     NVKPATTNPQATQEINGMISMIQTLVDKGYAYPAADGTVYFRVKKFKEYGKLSHKNLD
                     DLQSGFRSLQVSGEDQKEDPLDFVLWKPKKEGEPSWPSPWCDGRPGWHIECSVMAKKY
                     LGDEIDIHAGGEDLIFPHHENEIAQSECCNDKIFAKYWMHNAFLNIDNRKMSKSLGNF
                     RTVREISEQYDLQVLRFFMLNAHYRSPLNFSADLMEAAKNALERITDAAANLKDRKAA
                     AQTETATDAEKELLAQAQEFVKKFEEAMDDDFNTADALAAIFELVKFANTNVSEASSA
                     EFAGTLLDTMVKLCDVLGLKAVKTEEILDKEIEDLIAERQEARKAKNFARADEIRDEL
                     LAKGIILKDTREGVKWKRA"
     gene            2522..3001
                     /locus_tag="ACZPD3_05250"
     CDS             2522..3001
                     /locus_tag="ACZPD3_05250"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIR1415444.1"
                     /translation="METSLNDMSGTNGLNEENLSSLKGMLHQLFHLEDKDLRTYSPLT
                     LAYIGDGVYELVIRTILVKKGNCPVNQLHRKASSLVKAGTQSSMMEVIEPMLTEEEHS
                     VYRRGRNAHSPTMAKHATMADYRRATGFEALMGYLYLKDDFSRIIELVRAGIGEDKI"
     gene            2998..3735
                     /gene="rlmB"
                     /locus_tag="ACZPD3_05255"
     CDS             2998..3735
                     /gene="rlmB"
                     /locus_tag="ACZPD3_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR1415445.1"
                     /translation="MSEQIEGRNAVLEAFRSGKCVDKLFILDGCQDGPVRTIAREARK
                     KDTIINYVAKERLDQLSETGAHQGVIAQVAAYEYASVEDILAKAREKGEDPFIFILDN
                     IEDPHNLGAIIRTANLAGAHGVIIPKRRAVGLTSTVAKTSAGALNYTPVAKVTNLGHT
                     IDELKEQGMWFVCADMGGETMYNLNLTGPIGVVIGNEGEGVSRLIREKCDFVASIPMK
                     GDIDSLNASVAAGVLGYEIVRQRLQKK"
     gene            3891..>4421
                     /locus_tag="ACZPD3_05260"
     CDS             3891..>4421
                     /locus_tag="ACZPD3_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1415446.1"
                     /translation="MKQYDGIKDEELISRFKNGESEILDYLMEKYKNMVRKKARTMFL
                     IGGENDDLIQEGMIGLFKAVRDYQPDRDAAFQTFASICVDRQIYNAIQSSNRQKHQPL
                     NSYISLSEQDGENEEHLGDNWGENPESIIIDQENVQDLEQEITATLSPMENQVLEYYL
                     AGNGYGEIAQIMGKTPK"
BASE COUNT         1448 a          822 c         1127 g         1024 t
ORIGIN      
        1 gtgaaatctt cgattcagga tattacagag ggagaggaag atgaatgcga acagcttatg
       61 tatgcattga ttctttccgg acttgcaatg cagatgattg gtaattcaag gcctgcgtcc
      121 tgtgcagagc accaggtcac acatctgtgg gatatggaag tgatcaatgg tccgctggat
      181 gcactccacg gagagaaggt ttctgtagca gcacttcttg tactggaaga atacaagcgt
      241 atcgctgcag ccatcacaca gggaagatgt cacgcaaaac cctatgagaa tgaggacgaa
      301 gagcttctga aagaaacttt tggaaaaaaa ggacttctgg aagaaataag aaaagaaaat
      361 gagccggaat tgttagaaac tatttcaccc cagcatctgg aaaaatgcct gaacgggata
      421 gaggagatta ttgatgagct tcccagtgaa cagactatgt tccatttgct ggagaaagcc
      481 ggatgcgcga aaaccgttta tgatatcgga ctcgatgaag cggctgtttt gccaagcctc
      541 agacttgctc cttatacaag gagacggtta agcttattga gaatcagcaa gatgctggat
      601 ataagaggag aatgattatg cgtgttggaa tgggatatga tgtacacaga ctgaccgaag
      661 atagagatct gattctgggt ggtgtgaaga tagactggga aaaaggtctc ctgggacatt
      721 cagatgcaga tgtactgatc catgcagtta tggatgcact tctgggagct gctgcactgg
      781 gcgatatcgg aaaacatttt ccggatacag accctgcata taaaggaatt tccagtattc
      841 tgctgctgga acatgtgacg aagcttttga gagagcacca ttatgagatc ggaaatatag
      901 atgcaacgat cattgcacag aaaccaaaga tggcacctca tattccacag atgagagcca
      961 atatggcaaa agcaatggga atcaatgagt ctcagctgaa cattaaggcc actacagagg
     1021 aaaaactggg atttacggga agagaagaag gaattgcatc tcaggcgatc tgtcttctta
     1081 atgaaagaaa agagagttaa tgatagagaa aactggagga ataaaacatg aaacttttta
     1141 acacactgac acgtagaaaa gaagaattcg tacctcttga agagggaaaa gttcgtatgt
     1201 atgtctgcgg tccgactgta tacaacctga tccatatcgg aaatgcacgt ccgatgatca
     1261 tcttcgatac cgtacgccga tatatggaat acaaaggata tgaagtaaac tatgtttcca
     1321 attttacaga cgtagatgac aagatcatca agaaagcaat cgaagagggc gtaagcgcac
     1381 aggaaatttc cgagcgttat attgcagagt gtaagaaaga tatggatggc atgaatgtaa
     1441 aacctgccac aaccaatcca caggcaaccc aggaaatcaa cggaatgatc agtatgatcc
     1501 agactctggt tgataaggga tatgcttatc cggcagcaga cggtactgtt tatttcagag
     1561 ttaagaagtt caaagaatat ggcaaacttt cgcacaagaa cctggatgat ctgcagtccg
     1621 gcttccgttc tctgcaggtg tccggtgaag accagaaaga agatccactg gattttgttc
     1681 tctggaaacc gaagaaagag ggagaaccat cctggccttc accatggtgt gacggacgtc
     1741 cgggatggca tattgagtgt tctgttatgg cgaaaaaata ccttggtgat gagattgata
     1801 tccatgcagg cggagaagac ctgatctttc ctcaccatga gaacgagatc gcacagagtg
     1861 aatgctgcaa cgacaaaatc tttgcaaaat actggatgca caatgcattc ttaaatattg
     1921 ataacagaaa aatgtccaag tctcttggaa acttccgtac tgttcgtgaa atcagtgaac
     1981 agtatgacct gcaggtgctt cgtttcttca tgctgaacgc ccattacaga agtccgctga
     2041 atttcagcgc agatctgatg gaagctgcaa agaatgcatt agagcgtatc acagatgcag
     2101 cagcaaactt aaaggacaga aaagcagcag cacagacaga aactgcaaca gatgcagaga
     2161 aagaactgct tgcacaggca caggaattcg taaagaaatt tgaagaagct atggatgatg
     2221 atttcaacac agcagatgca ctggcagcaa tctttgaact ggttaaattt gcaaatacaa
     2281 atgtatcaga ggcaagttcc gcagaatttg caggaacact tcttgacaca atggtgaaat
     2341 tatgcgatgt acttggtctg aaggctgtga aaacagaaga aatccttgat aaagaaatcg
     2401 aagacctgat tgcagagcgt caggaagcaa gaaaagcaaa gaatttcgca agagcagatg
     2461 agatcagaga tgagcttctg gctaagggaa tcatccttaa ggatacccgc gagggagtaa
     2521 aatggaaacg agcttaaatg atatgagtgg aacaaacgga cttaatgaag agaatttgag
     2581 tagtttgaaa ggcatgcttc atcagttgtt tcatttggaa gataaagact tgcgaacata
     2641 ttctccgctg acattggcgt atatcggaga tggcgtctat gaactggtga tccgcacgat
     2701 ccttgtaaaa aaaggcaact gtccggtgaa ccagcttcac agaaaagcca gcagtctggt
     2761 taaggcagga actcagtctt ccatgatgga agtaatcgag cccatgctta cagaggagga
     2821 acacagcgtt tacagaagag gaaggaatgc tcattctcct acaatggcta aacatgcaac
     2881 catggcagac taccgcaggg caacagggtt tgaagctctg atgggctatt tatatttaaa
     2941 agatgatttc agcaggatca tagaactggt ccgtgcaggg attggagagg ataagatatg
     3001 agtgaacaga tagaaggacg caatgcggtt ctggaagcgt ttcgttccgg caaatgtgta
     3061 gataagcttt ttatcctgga cggatgccag gacggaccgg tccgcactat tgccagagag
     3121 gcaagaaaaa aagatacgat tattaattat gtggcaaaag aacgtctgga tcagttaagc
     3181 gagacaggcg cacatcaggg cgtaatcgct caggtggcag cctatgaata tgcaagtgta
     3241 gaagatattc ttgcgaaggc aagggaaaaa ggagaagatc cctttatctt tatcctggat
     3301 aatattgagg atcctcataa tcttggtgcg atcatccgta cagcaaacct tgcaggtgca
     3361 catggggtga ttattcccaa gagaagagca gttggcctga cttccacagt tgccaagact
     3421 tcagcaggtg cattaaacta tacacctgtt gcaaaagtta ccaatctggg acataccatc
     3481 gatgaactga aagaacaggg catgtggttt gtatgtgcag atatgggcgg tgaaaccatg
     3541 tacaatctta acctgacagg tccgatcggc gtagtgatcg gcaatgaagg tgaaggtgta
     3601 agcagactga tccgtgagaa gtgtgatttc gtggcatcta ttcctatgaa gggtgacatt
     3661 gattccctca atgcatctgt ggcagccgga gtacttggat atgagattgt acgtcagaga
     3721 ctgcagaaga aataagttta tctcagtctg agataaaggc ttcgtgagga tgtgcagcga
     3781 ttattaagag gaatttttgc agcagaaaat tattccacaa tattgttatg ctataagaag
     3841 ccctctgggc gaatgataga gattttcaga aaaacggaag ggttaaattg atgaaacagt
     3901 atgacggaat taaagatgaa gaattaattt cccgtttcaa aaacggggaa tcagaaatcc
     3961 tcgactatct gatggaaaaa tacaagaaca tggtccgcaa gaaagcaagg accatgttcc
     4021 tgataggcgg ggagaacgat gacctgatac aggaaggcat gatcggactg tttaaggcag
     4081 tccgcgatta ccagccggac agggatgctg cattccagac ctttgccagt atatgtgtgg
     4141 acagacagat ctacaacgcc atccagagtt ccaacaggca gaagcaccag ccactgaatt
     4201 cctatatttc cctcagcgaa caggatgggg aaaatgaaga acatcttgga gataactggg
     4261 gagagaaccc ggagtctatc attattgacc aggagaatgt ccaggatctg gagcaggaga
     4321 taacagcaac gttaagtcca atggagaacc aggttcttga atattatctg gcaggaaacg
     4381 gatacggaga gattgcccag atcatgggaa aaacacccaa a
//
LOCUS       DBMDXG010000149         4389 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4776_length_4389_cov_1.744636, whole genome shotgun sequence.
ACCESSION   DBMDXG010000149 DBMDXG010000000
VERSION     DBMDXG010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_4389_cov_1.744636"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="ACZPD3_05265"
     CDS             <1..280
                     /locus_tag="ACZPD3_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415447.1"
                     /translation="GTAGKELIKVLPDYEYEKITNTTDVSLHRDEPHEYKDGRHHRNG
                     GGVRFSQSKSLESRCIFSQDDLVKMQDSNSMLIYNARYAHTWVAKIVG"
     gene            406..2376
                     /gene="ftsH"
                     /locus_tag="ACZPD3_05270"
     CDS             406..2376
                     /gene="ftsH"
                     /locus_tag="ACZPD3_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1415448.1"
                     /translation="MDNHMDNNPNGNRPDNNKGPGRQDPNKNHQSILAFLVCLLVTLV
                     CFAMFTNMLKDNSSEITYDKFIEMVDDGDVKEVTLQSNTLTITPKHQSSGFSEEVYYA
                     NQMESVDKLTERLEGTGIKFEYKKPDAAGEIISMLVSVLLPTILLFVLLTVFMRRMNK
                     GGGMMGVGKSRAKAYIQKDTGITFRNVAGQDEAKESLQEVVDFLHNPGKYTTIGAKLP
                     KGALLVGPPGTGKTLLAKAVAGEAHVPFFSLSGSEFVEMFVGVGASRVRDLFEEAKKN
                     APCIIFIDEIDAIGKSRDSHYGGGNDEREQTLNQLLAEMDGFDTSKGLLILAATNRPE
                     VLDPALLRPGRFDRRVIVDRPDLKGRIEILKVHARNVHLDETVDFENIALATSGAVGS
                     DLANMINEAAILAVKSGRSAVSQKDLLEAVEVVLVGKEKKDRILSAQERRIVSYHEVG
                     HALVSALQKDAEPVQKITIVPRTMGALGYVMQVPEEEKYLNTKKELEAMLVGYLGGRA
                     AEELVFDTVTTGAANDIEQATKVARAMITQYGMSDKFGLMGLATQENQYLSGRTVLNC
                     GDDTATEVDHEVMVLLHNSYEEAKRLIGSHREALDKIAAYLIRRETITGKEFMKIFHA
                     AERGIEIPENLDDLVIPEEKQNTVTLDKPEIQ"
     gene            2502..4367
                     /gene="uvrC"
                     /locus_tag="ACZPD3_05275"
     CDS             2502..4367
                     /gene="uvrC"
                     /locus_tag="ACZPD3_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR1415449.1"
                     /translation="MFQIEEELKKLPGKPGVYIMHGEKDEIIYVGKAVSLKNRVRQYF
                     QSSRNKGAKIEQMVTHITRFEYIITDSELEALVLECNLIKEHRPKYNTMLKDDKSYPF
                     IKVTVNEEYPRVLFARRMKKDKAKYFGPYTSAGAVKDVIELVRKLYKVRSCNRVLPRD
                     CGKDRPCLYYHMKQCSAPCQGYVSSEEYKKNIAELLKFLNGDFKDTIDMLTDKMMAAS
                     EEMRFEDAMEYRDLIRSIQKIGERQKITGYGEEDKDIIAVAMDESLDLREQDAVVQVF
                     FVRGGKLIGREHFYLRVARGDTKAQVLSSFMKQFYAGTPFIPREIMLQKEIEDAKIIE
                     EWLTDRRKQRVYIRVPKKGTKEKLVELAEENAKMVLDKDRERIKREEGRTIGAVHEVE
                     EWLGLSGIRRMEAYDISNISGFESVGSMVVYEKGKPKRSDYRKFKIKWVQGPNDYASM
                     EEVLTRRFTHEGKDEFDSFSIMPDLILMDGGRGQVNIALKVLEILGIEIPVCGMVKDD
                     HHRTRGLYYNNLEIPIDTDSEGFRLITRIQDEAHRFAIEFHRSLRSKEQVHSLLDDIP
                     GIGETRRKALMRKFKSVENIRDASLEELTETESMNAGSAQKVYEFFHGASTSFSK"
BASE COUNT         1362 a          782 c         1160 g         1085 t
ORIGIN      
        1 tgggacagca ggaaaagaat taataaaagt attgcctgat tatgagtacg aaaaaatcac
       61 gaatactacg gatgtgtctt tgcatcgtga cgaaccacat gaatataaag atggaagaca
      121 ccataggaat ggtggaggag ttcgttttag tcagtctaaa tcattggaat ctcgttgtat
      181 tttttcacaa gatgatttag tcaaaatgca ggattcaaat tctatgctga tttataatgc
      241 acgttatgca catacctggg tggcaaaaat agttggctga taataaagtt cctttagaat
      301 aaaaagaaat tcacagttga attcctgata aatttcacaa tctcgcggtt gatttatagc
      361 ataattctct ttataataag aacgataacg tttaggagga atgaaatgga taatcatatg
      421 gataacaatc cgaatggcaa tcgtcctgat aataataaag gtccgggcag acaggacccg
      481 aacaagaatc atcagtcgat actggccttt ctggtatgcc tcctggtaac tctggtatgt
      541 ttcgccatgt ttaccaatat gctcaaggat aattcgagtg agatcactta tgataaattt
      601 attgagatgg tagatgatgg agatgtgaaa gaggttacgc ttcagtcaaa tacactgacg
      661 atcacgccga aacatcagtc atcaggattt tcagaagaag tttattatgc caatcagatg
      721 gagtcagtag ataagctgac ggagagactt gagggaacag gaatcaagtt tgagtataag
      781 aagccggatg cagcagggga aattatttct atgctggtca gtgtgcttct gccaacgatt
      841 ctgctctttg tacttctgac ggtatttatg cgccgcatga ataagggcgg tggaatgatg
      901 ggcgttggaa agagccgcgc caaagcctac attcagaaag atacaggaat tactttcagg
      961 aatgtggcag ggcaggatga ggccaaggaa tcactgcagg aggtggtgga tttcctgcat
     1021 aatcccggca agtataccac catcggagcg aagctgccga aaggagcttt gcttgtagga
     1081 cctccgggaa ccggtaaaac acttctggca aaggcagtag caggagaggc acatgtgccg
     1141 tttttctctc tgtcaggttc tgagtttgtg gagatgtttg taggtgtggg tgcatcacgt
     1201 gtccgcgatc tctttgagga agcaaagaag aatgcaccat gtatcatttt tattgatgag
     1261 attgatgcaa tcggaaagag ccgagattct cattatggcg gcggcaatga tgagcgcgag
     1321 cagaccctga accagcttct tgcggaaatg gacggatttg acacttcaaa gggacttctg
     1381 atcctggctg ctaccaaccg tccggaggtt cttgatccgg cacttcttcg tccgggacgt
     1441 tttgacagac gtgtcattgt ggacagaccg gatctgaagg gaagaatcga gatcctgaag
     1501 gttcatgcaa gaaatgtaca tctggatgag actgttgatt ttgagaacat tgcacttgca
     1561 acttccgggg cagtgggttc ggatctggct aatatgatca acgaagctgc tatccttgct
     1621 gttaagagtg gacgcagcgc agtatcccag aaagatcttc tcgaagctgt tgaagtagtt
     1681 ctggtaggta aagagaagaa ggacaggatc ctcagtgcac aggagcgcag aatcgtatcc
     1741 tatcatgagg taggacatgc actggtaagc gccctgcaga aagatgcaga gcctgtacag
     1801 aagattacta ttgtgccgag aacaatgggg gcacttggtt atgtaatgca ggtacccgag
     1861 gaggagaaat atctcaatac aaagaaggaa ctggaagcaa tgcttgtggg atatcttggc
     1921 ggacgcgcag cagaggaact ggtatttgat actgtcacaa ccggggcagc caatgatatt
     1981 gagcaggcta ccaaggtggc aagagccatg atcacacagt acggtatgtc cgacaagttc
     2041 ggacttatgg gtctggctac acaggagaat cagtatctca gcggaagaac agtgttaaac
     2101 tgtggcgatg acactgccac agaggttgac catgaggtaa tggtacttct gcataattcc
     2161 tatgaggaag ccaagagact gatcggcagc cacagagaag cacttgataa gattgctgct
     2221 tacctgatcc gcagggaaac cattacaggt aaggaattta tgaagatttt ccatgcggca
     2281 gagcgtggaa tcgagattcc ggaaaatctg gatgatctag taattccaga ggaaaaacag
     2341 aatacagtaa cgctggataa gccagagatt cagtgataca ataacagagt cggaaatgaa
     2401 ataatttccg gctctgtttt cgtatcacag aaaattacgc tgtaatatct catggatacg
     2461 aaaagcgttc agggcaggat tttttgtata gaggagagtt tatgtttcag atagaagaag
     2521 aactgaaaaa acttccgggt aagccggggg tttatattat gcatggggag aaggacgaga
     2581 tcatctatgt tggaaaagcc gtcagtctga aaaacagggt gcgtcaatat ttccagagca
     2641 gcagaaataa gggcgcaaag attgaacaga tggttaccca tatcaccaga tttgagtata
     2701 ttatcacaga ttcggaactg gaagcccttg tgctggagtg taatctgatc aaggagcaca
     2761 gaccgaaata taataccatg ctcaaagatg ataagagtta tccgtttatc aaagtcacag
     2821 taaatgagga atatcccaga gttctttttg ccagaagaat gaagaaagac aaggcaaaat
     2881 atttcggacc atatacaagt gccggggctg taaaagatgt gatcgagctt gtgcgaaaac
     2941 tttataaggt gcgatcctgt aatcgggtat tgccaagaga ttgtgggaag gaccgtccat
     3001 gtctgtacta tcacatgaaa cagtgcagtg cgccatgcca gggctatgtg agttcagaag
     3061 aatataagaa aaatatcgca gaactgctga aatttctcaa tggagacttt aaggatacga
     3121 ttgatatgct taccgacaaa atgatggcag cttccgaaga aatgcgtttc gaggatgcca
     3181 tggaataccg ggatctgatc cgcagtatcc agaagatcgg agaacgtcag aaaattacag
     3241 gttacggaga ggaagataag gatatcatcg cagttgccat ggatgaaagc cttgatctgc
     3301 gggagcagga tgctgttgta caggtattct ttgtccgggg aggcaaactg atcgggcggg
     3361 agcatttcta tttgcgtgtg gcgcgtggcg atacaaaggc tcaggtatta tccagcttta
     3421 tgaagcaatt ttatgccgga actccgttta tcccacgtga gatcatgctg caaaaagaaa
     3481 tcgaagatgc caagatcata gaggaatggc ttacagatcg aagaaagcag cgagtctata
     3541 tcagggtacc gaagaaaggt acgaaagaga agctggtaga gctggcagaa gagaatgcaa
     3601 agatggttct ggataaagac agagagcgta tcaaacgtga agaaggaaga accatcggag
     3661 cagttcatga agtggaagag tggcttggac tgtcgggaat caggcgtatg gaggcttatg
     3721 atatctccaa tatcagtggt tttgaatctg taggttccat ggttgtctat gagaaaggaa
     3781 agccaaagcg cagtgattac cgcaaattta aaataaagtg ggttcaggga ccgaacgatt
     3841 atgcaagcat ggaagaagta cttaccagac gatttactca tgagggaaaa gatgagtttg
     3901 acagcttttc tattatgccg gatctgatcc tgatggatgg tggaagagga caggtgaata
     3961 ttgcgttaaa agtcctggag attctgggaa tagagattcc tgtctgtggt atggtcaagg
     4021 atgaccatca cagaaccaga ggactgtatt acaataatct tgaaattccc attgatacag
     4081 acagtgaagg cttccgcctg atcaccagaa tccaggatga ggcgcaccgt tttgccattg
     4141 aatttcaccg atctctgaga agtaaagaac aggtgcattc cctgttggat gatatccctg
     4201 gtatcggtga aaccaggcgg aaagcattga tgcggaaatt taagtctgtt gagaatatca
     4261 gagatgcatc attggaagaa cttacggaga cagaaagcat gaatgcagga agtgcacaga
     4321 aagtctatga attttttcat ggagctagta catcgttttc gaaataacta taccactcac
     4381 ttgtctgcg
//
LOCUS       DBMDXG010000150         4387 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4780_length_4387_cov_1.579561, whole genome shotgun sequence.
ACCESSION   DBMDXG010000150 DBMDXG010000000
VERSION     DBMDXG010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4780_length_4387_cov_1.579561"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="ACZPD3_05280"
     CDS             <1..467
                     /locus_tag="ACZPD3_05280"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1415450.1"
                     /translation="SYEKNKKGESYCKTNNVIKKEKLLLKRNHRLTNIRKNYLNQTIS
                     EIINRKPRFICIEDLNVSGMMKNRHLSKAVQEQGFFWFRKQLEYKCSDKGIQLIVADR
                     FYPSSKLCSCCGNIKKDLKLSDRVYRCACGNMIDRDFQASINLKTYGEKFAG"
     gene            614..1510
                     /locus_tag="ACZPD3_05285"
                     /pseudo
     CDS             614..1510
                     /locus_tag="ACZPD3_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706795.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     gene            1738..2358
                     /locus_tag="ACZPD3_05290"
     CDS             1738..2358
                     /locus_tag="ACZPD3_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1415451.1"
                     /translation="MKHLVITIGCEYGAKGNAIGKKIAQDLGIKFYDRDLVDEIIKEV
                     GIPTDIMDRVEEGGTIAGKGAQGDVRGSFSKYADLTDRAIHVQKQIIRKLAQKEACVI
                     IGRSADYILKDTEGIQLVRAFIYAPNDVRVHNIMESHNLSEKDAKLLLDEKDKRYHKR
                     HLALTGSNRGDRHNRDILINSDFLGIQGTAEYLEDLIAKKYGKKEA"
     gene            2362..3882
                     /locus_tag="ACZPD3_05295"
     CDS             2362..3882
                     /locus_tag="ACZPD3_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR1415452.1"
                     /translation="MEQKTSEFDKVLGAWDILVIAFGAMIGWGWVVSSGNWIESGGVL
                     GAAIAFAIGGIMIFFVGLTYAELTAAMPQCGGEHVFSYRAMGSTGSFICTWAIILGYV
                     SVACFEACAFPTIITYLWPGFLKGYLYTVAGFDVYASWLIVAIVIAFLIMIININGAK
                     TAAVLQTVLTCIIGGAGIILIVASVVTGSVDNLQGQMFVGNGTGEAMKSIIKVAVITP
                     FFFIGFDVIPQAAEEINVPLKKIGKMMILSVVLAVVFYSLVILAVGFILNPAEIIESQ
                     QGTGLVTADAMAKAFGTKIMAKVIIVGGMCGIITSWNSFLLGGSRAMYSMAESYMIPP
                     FFAKLHPKHKTPVNSLYLIGALTMLAPFAGRTMMVWICDAGNFGCCLAYCMVSVSFLI
                     LRKKEPDMARPYKVGHYKFVGFMAVVMSGLMLLVYCIPGSGGSLVFQEWFMVGGWSLL
                     GVVFYAICKRKYKEDFGKLIELISDEDAASLMPEADDEELDVVIDAAIDRVLSSMA"
     gene            3994..>4387
                     /locus_tag="ACZPD3_05300"
     CDS             3994..>4387
                     /locus_tag="ACZPD3_05300"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1415453.1"
                     /translation="MNPFEFFIPQNITVGAGTLAKLPECAKKLGGSHAMLISGPTLRK
                     MGVVDKAADYLKDAGMAVDIFTDVEANPSVTTVEKATEAYKDSGADFIVALGGGSPMD
                     VAKAVGVTAKYGGILEIHIDQKDGPCVGT"
BASE COUNT         1283 a          768 c         1109 g         1227 t
ORIGIN      
        1 gttcttacga gaaaaataag aaaggggaaa gttactgtaa aacaaataat gtaatcaaaa
       61 aggaaaaact tttattaaaa cgaaatcaca gattaacaaa tatccgtaaa aactatttga
      121 atcagaccat atctgagatc ataaatcgaa aaccaagatt tatatgtatt gaagatctga
      181 atgtcagcgg aatgatgaaa aacagacatt tatccaaagc agtccaggaa cagggatttt
      241 tttggtttag aaaacagctt gaatataagt gcagcgataa agggatccag cttattgtgg
      301 ctgatcggtt ttatccatca tcaaaacttt gcagctgttg tggaaacatc aaaaaagatc
      361 tgaagttatc tgacagagtt tatagatgtg cgtgtgggaa tatgattgac agagatttcc
      421 aggcatctat aaatctcaag acttatggag aaaaatttgc aggctgacac tgaaacgtta
      481 atgcaaatat gtacggatac gttagtccgg aatttacgcc tatggagagt acaggaactt
      541 gtgagtagat tgatatttat atcatcaaaa gcatactcgt tgaagtagga atggaacata
      601 gaagtttata actttttata agttttcagt aacggctaac gaggcaatga tcaaaatggc
      661 ccgtaaatat ggtattgaaa agtatggtcc aaaccgttat catatcgtaa ctgcaaagat
      721 gggattccat ggaagaacct ttggtgccat gtctgcaaca ggtcagccgg gtaatggatg
      781 ccaggttgga ttcggaccta tgacttatgg tttctcctat gcaccgtaca atgaccttca
      841 ggctttcaag gatgcatgca cagagaacac aattgccatt atggttgagc ctgtacaggg
      901 cgagggtggc gtacatcctg caacaatgga attcatgcag ggacttcgta aattctgtga
      961 tgagaacgat atgcttcttc tgattgatga agtacagaca ggatggtgca gaacaggtgc
     1021 tgttatgtcc tatatgaatt atggtatcaa accggatatc gtatctatgg caaaggctct
     1081 gggcggtgga atgccgatcg gtgccatctg tgcaacagca gaggttgcaa aagctttcac
     1141 agcaggttcc catggaacta cattcggcgg acatccggta agctgtgcag ctgcactggc
     1201 tgaggtgaat gagcttctgg acagagattt ggctggaaat gcaaagaagg ttggcgatta
     1261 ctttgcagct aagcttgaga agcttcctca tgtaaaagaa gtaagacatc agggacttct
     1321 ggtaggtgtg gaatttgatg atactatcgg cggagtggat gtaaaacatg gttgtcttga
     1381 cagacatctt ctgatcacag ctatcggagc gcatatcatc cgtatgatcc caccgcttat
     1441 tgttacagaa gaagagtgtg acaaagctgt agcaatcatc gaagatacaa ttaaatctct
     1501 ggaaaaataa aaatccggca acggagtgat acagtaagga caagggcgtc acgaattata
     1561 ttcgtgcgcc cttttatgat atcagggccc aaaggtcaaa aagtcgttcg tgcctgcacg
     1621 ataagacttt tgaacttggg acctgccaaa catgagaaag tagcgattta catagcaaat
     1681 cagtggattt caaatgtttg agaatctgat aaaaatttga gtgtgagagg gaagagcatg
     1741 aaacatctgg taattacgat tggctgcgaa tatggagcca agggcaatgc catcggtaag
     1801 aagattgcac aggatctggg gattaagttt tatgatcgtg atcttgtgga tgagattatc
     1861 aaagaggtag gcatccctac agacattatg gacagagtag aggaaggcgg aaccatcgca
     1921 ggtaaaggtg cacagggtga tgtgagaggt tccttttcca aatatgcgga tctgacagac
     1981 cgcgccattc atgtacagaa gcagattatc cggaagctgg ctcagaaaga agcctgcgtg
     2041 atcataggac ggtctgcaga ttatattttg aaagatacag aaggaatcca actggtgaga
     2101 gcatttattt atgcacctaa tgatgttcgc gtccacaaca ttatggaaag ccataatctt
     2161 tcggaaaagg atgcaaagct tctgctggat gagaaggata aacgttatca taagagacat
     2221 ctggcactga caggaagtaa ccgcggtgac agacataaca gggatattct tattaacagt
     2281 gattttctgg gaattcaggg aacagcagaa tatctggaag atttgattgc aaaaaaatac
     2341 ggaaaaaagg aggcctgacc tatggaacaa aaaacatcag aatttgacaa agtacttggt
     2401 gcatgggaca tacttgtaat tgcatttggc gcaatgatcg gctggggctg ggtagtttct
     2461 tccggtaact ggattgaatc cggtggtgtg cttggtgcag ctattgcatt tgcaatcggt
     2521 ggaatcatga tcttctttgt aggtctgact tacgcagagc ttacagcagc catgcctcag
     2581 tgtggtggtg agcacgtatt cagctacagg gcaatgggtt ctacaggttc ttttatctgt
     2641 acctgggcga tcattcttgg atatgtaagt gttgcctgct ttgaggcatg tgcatttccc
     2701 acaatcatca cttatctgtg gccggggttc ctgaaaggat atctctatac agttgcaggc
     2761 tttgatgttt atgcatcctg gctgatcgtg gcaattgtga ttgcttttct tattatgata
     2821 atcaacatta atggagcaaa aactgcagca gttttgcaga ctgttcttac ctgtattatc
     2881 ggcggtgccg gaattattct gatcgtggct tctgtggtta caggaagtgt ggataatctt
     2941 cagggacaga tgtttgttgg aaacggtact ggcgaagcaa tgaaaagtat tattaaggtt
     3001 gcagttatca ctccgttttt ctttattggt tttgacgtta tcccacaggc agcagaggaa
     3061 atcaatgttc cgctgaaaaa aatcggtaaa atgatgatcc tctctgttgt gctagcagtt
     3121 gtattttact cactggtgat tcttgcagta ggttttatcc tgaatccggc agagatcatt
     3181 gagtctcagc agggaacagg acttgtaacg gcagatgcca tggccaaggc tttcgggaca
     3241 aagattatgg ccaaggttat cattgtaggt ggaatgtgtg gtattattac atcatggaac
     3301 tccttcctcc ttggcggttc ccgtgccatg tactctatgg cagaatccta tatgattcct
     3361 ccgttttttg caaagcttca tccgaagcac aaaactccgg ttaattctct gtatctgatc
     3421 ggtgccctga ctatgctggc tccttttgca ggaagaacaa tgatggtctg gatctgtgat
     3481 gcaggtaact tcggatgctg ccttgcatac tgtatggtct ctgtctcttt cctgatcctt
     3541 cgtaaaaagg aaccggatat ggcaagacct tacaaagtag gccactacaa atttgtaggt
     3601 tttatggcag tagtcatgtc agggctgatg cttctggttt attgtattcc gggttccggt
     3661 ggaagtctgg tatttcagga atggtttatg gtaggtggct ggagtttact tggagttgta
     3721 ttttatgcga tctgtaaacg taaatataaa gaagactttg gtaaacttat tgaactgatc
     3781 tctgatgagg atgcagcaag tcttatgccg gaggctgatg atgaagagct tgatgtggta
     3841 atcgatgcgg ctattgacag ggtgctttcc tctatggcat aacgctataa atgacatgac
     3901 gattattttg ggtaacagag ttttgaattt gtgcagggta gatgccctgc gcattgcaat
     3961 attgaaaaaa tacaggaaaa gaggcagaaa ttaatgaatc catttgaatt ttttattcct
     4021 cagaatatta cagtcggtgc aggtactctg gcgaagcttc cggaatgtgc caaaaagctt
     4081 ggaggttctc atgcaatgct gatctcagga ccaactctcc ggaaaatggg agttgtggat
     4141 aaggcggcgg attatctgaa agacgccgga atggcagtgg atatttttac agatgtagaa
     4201 gccaatccgt ctgtcaccac agtggaaaaa gcgacagaag cgtataagga ttccggcgca
     4261 gattttatcg ttgcgctggg cggtggttct cctatggatg tggcaaaagc agtgggagtt
     4321 acggctaaat atggcggcat attggaaatt cacattgacc aaaaagatgg tccatgtgtc
     4381 ggcacag
//
LOCUS       DBMDXG010000151         4386 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4781_length_4386_cov_1.694982, whole genome shotgun sequence.
ACCESSION   DBMDXG010000151 DBMDXG010000000
VERSION     DBMDXG010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4781_length_4386_cov_1.694982"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="ACZPD3_05305"
     CDS             <1..631
                     /locus_tag="ACZPD3_05305"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR1415454.1"
                     /translation="DTAEDSVIMTRSFTSMLYMAVIMAMYVGGKKDEIAAMKDYAPYS
                     EKVLKEMDELSTKIMNEHKNLNLYIILGQGIYYGVANECMNKMKEMGLSNSEGYYDLE
                     YRHGPMSLVDENTLIVCLSNKACRDGDKALMEQMKSYGATVAVLGGELGDTFDQIADY
                     VYSMDKGWNDMQYSAIIGFIGQLLGYYIADSKNLDADSPRHLTQAIVLK"
     gene            complement(747..1805)
                     /locus_tag="ACZPD3_05310"
     CDS             complement(747..1805)
                     /locus_tag="ACZPD3_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1415455.1"
                     /translation="MTLKEIARQANVSVSTVSRVINQKNTKAASPEVQERIWKIVRKS
                     GYTPNATARSLKLGQKAQIQNTTPKSIACIYARSNSAVSDPFFSQIAKAIEQEAFKSN
                     YFIRQSFTAMDIANPDMARQLAQFPVDGIVILGRYEKQLLRFFTQNYKNVIYTGLNPM
                     GTQYDQVVCDGSDISYSALSYLVELGHTKIGYVGEQNNEVRFSGYKDALAKLGLPFLQ
                     KNTSNVHLSADGGYKGANILMHNKADISAIFCANDTTALGVIHALKEKNFRIPEDISV
                     ISVDDIETAQYLSPMLTTVHIPLEELGRITAKTLIDRINGGHRLPMRISLPFYIAKRD
                     SCGKLNRRRKTFASLPPQ"
     gene            2080..2937
                     /gene="larC"
                     /locus_tag="ACZPD3_05315"
                     /pseudo
     CDS             2080..2937
                     /gene="larC"
                     /locus_tag="ACZPD3_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     gene            3070..3891
                     /locus_tag="ACZPD3_05320"
     CDS             3070..3891
                     /locus_tag="ACZPD3_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase family"
                     /protein_id="HIR1415456.1"
                     /translation="MGHSYYSYTKEELVGNGPKLPVIVMEDNAEVFHSMAVEMVDEIR
                     KHNEKGEKTVFICPVGPVGQYPYFVDMVNEQNISLKNVWFINMDEYLDDEKQWISKDH
                     PLSFRGFMQRTVYDKVRPELVMPEDQRVFPDPNHPEYISELIEKLGGVDICFGGIGIN
                     GHVAFNEADPSLTNEEFLAQKTRVLKITKETRTANAIGDFNGALEDMPHYCITIGINE
                     ISHARKIRLGCFRNWHRAVVRRAAYGEATSDFPVSLLTSHPDINLKITEFVAALD"
     gene            3928..>4386
                     /locus_tag="ACZPD3_05325"
     CDS             3928..>4386
                     /locus_tag="ACZPD3_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1415457.1"
                     /translation="MSIRIINGKVYKNGAFSEEEIVLKEGKIVSAQDGDAEEVYDAKG
                     NYVVPGFIDVHTHGGAGVDVNAADQQGFEKIGHFFATQGTTSWLSSILTDTKEQTLKT
                     IKEAVKHQEAQKNGHENCADLLGIHLEGPFLSPEYKGAMPEHLLISPDLDL"
BASE COUNT         1411 a          720 c         1006 g         1249 t
ORIGIN      
        1 ggatacagct gaagacagtg ttatcatgac acgttctttt acaagtatgt tatatatggc
       61 tgttatcatg gctatgtatg taggcggtaa gaaagatgaa attgctgcaa tgaaagatta
      121 tgctccatat tctgaaaagg ttctgaaaga aatggatgaa ctttctacaa aaatcatgaa
      181 tgagcataaa aacctgaatc tctacattat tcttggacag ggtatttatt atggtgtcgc
      241 caatgaatgt atgaataaga tgaaagaaat gggactcagc aattctgaag gatattatga
      301 tctggaatac cgtcatggac caatgagtct tgtagatgaa aatacactga tcgtgtgctt
      361 gagcaataaa gcctgcagag atggtgacaa agcacttatg gagcagatga agagttacgg
      421 tgcaacagtt gcagttcttg gcggagaact gggagatacc tttgatcaga tcgctgatta
      481 tgtatattcc atggataaag gctggaatga tatgcagtac agtgcgatca tcggatttat
      541 cggacagctt ctgggatatt atattgcaga cagcaagaat ctggatgcag attcaccgag
      601 acacctgaca caggctattg tattgaaata atttaagaaa actgaggagc agattgcgaa
      661 tatctgcctg attcaaactg cgaagcagtt atagtattaa agaaaagact cccggcagga
      721 agaagtgctg ccgggagtct tttctattac tgaggaggaa gacttgcaaa agtcttacgt
      781 cttctgttaa gttttccgca actgtctctt ttggcaatgt aaaatggaag agagatacgc
      841 atgggaaggc gatgtccgcc gttaatacgg tcgatcaggg tttttgcagt tattcttcca
      901 agttcctcca gaggaatatg tactgtggtc agcatgggag aaagatactg agctgtctct
      961 atatcatcta cgctgataac agaaatatct tccggtattc gaaaattttt ttcctttaat
     1021 gcgtgaatga ctccgagggc agtggtatcg tttgcacaga aaattgcaga gatatctgcc
     1081 ttattgtgca tcaggatatt agcacctttg taaccgccat ctgcagaaag atgtacatta
     1141 ctggtatttt tctgcaaaaa cggaagccca agtttggcaa gtgcatcttt ataaccgctg
     1201 aaacgtactt cattattctg ttcacctaca taaccgattt tcgtatgtcc gagctccaca
     1261 agataggaaa gagcactata ggaaatatca cttccgtcac aaactacctg gtcatattgg
     1321 gttcccattg gattcagacc tgtataaatc acatttttat aattttgagt aaagaagcgt
     1381 aacagctgtt tctcatatcg tcccagaatt acgattccgt ctactggaaa ttgagcaagc
     1441 tgtcgtgcca tatcgggatt tgcaatatcc atggctgtaa atgactgacg gataaaatag
     1501 ttgctcttga aggcctcctg ttcaatcgct tttgcaatct gtgagaagaa tggatcggaa
     1561 acagcggaat tacttcttgc ataaatacag gcaatggatt tgggcgttgt attctgaatt
     1621 tgagcttttt gtcctaattt gaggcttcgg gctgttgcat tgggagtata accgcttttg
     1681 cgtacgattt tccatatacg ttcctgaact tccggacttg ccgcttttgt atttttctgg
     1741 tttattactc tggaaaccgt agaaacagag acatttgcct gcctggcaat ttcttttaat
     1801 gtcataataa ctccccgcaa tcttttatac gactattttc taaaactatg tgcaaaacag
     1861 aaaatcagag tcttctgttt taaaaaagtc gttcagaacc ccgccggggg gttctgaacg
     1921 actttaatta tacagtaaat ttataaaaaa accaataaaa agaaggaaaa cgtttgcgta
     1981 ctttgaatgc aaaatcaact caaagttatt gataaaaaag taaaaactta tataattttt
     2041 gatagagtac ataaaactaa agtggaggtg cttaaattta tggggaaaac attgtattta
     2101 gagtgctatt cagggattag tggtgatatg actgtcggtg cacttcttga tctgggggcg
     2161 gataaggcag ttttggacag ggtactgcaa agtcttcctg tatcagggtt tcagataaaa
     2221 accggcaaag tggtaaaatc cggaattaaa gcctgtgatt ttgacgttat gttagataaa
     2281 gatcatgaaa accatgatca tgatatgaag tatctgcatg gtcataatga gaatactgag
     2341 ggagagtctg agattcatac acatgaacat gttattgcag accatgcatg tgcacatgag
     2401 agagaaactg cacattgtca ccatcaccat gaacatagag gggtgaaaga aattgcacat
     2461 attatagaac atagtgcaat gactgacaat gcaaaaaaga ttgctttgag aatatttgag
     2521 attcttgcaa aagcagagag caaggcacat gatgtgcctg tggagcaggt acattttcat
     2581 gaagtcgggg cagttgattc cattgtagat attgtatctg tggcagtctg tctggataat
     2641 ctggatatta cggatgtagt tgtacctgta ctttatgaag gctgcggaac tgtccgctgt
     2701 cagcatggaa ttttaccaat tcctgttcct gcagttgcga atgtgataag cgaaaatcat
     2761 ttgtgtataa aggtgacagc tgttgagggg gaacttgtaa cacctacagg tgctgctatt
     2821 gtggcagcaa taagaacgaa agataagctt ccggaggaat ttgaaataaa gaaaataggg
     2881 attggagccg gaaaacgtca gtatgaatgt cccggaatcc tcagagcaat gattatttct
     2941 tagaaaacgt ttgcgcagat tccaggcaaa tttgcatgga attcgttgat taaaaaaatt
     3001 ttatctacta taataaatac aacaataaag gtaacgagct ggataataaa tgaaaggaag
     3061 taaagcaata tgggacatag ttattatagc tatacaaagg aagagttagt gggtaatgga
     3121 ccgaagcttc cggtaattgt tatggaagac aatgcagaag tatttcattc tatggcagtt
     3181 gaaatggtgg atgagatcag aaaacataac gaaaagggag aaaaaacagt atttatctgt
     3241 cctgtaggtc ctgtaggaca gtatccatat tttgtagata tggttaatga gcagaatatc
     3301 agcctgaaaa atgtatggtt tattaacatg gatgaatatc tggatgatga gaaacaatgg
     3361 atttcaaaag atcatccgtt aagcttcaga ggcttcatgc aacgtacagt ttatgataaa
     3421 gttcgcccgg aattggtaat gccggaagat cagagagtat ttccggatcc gaatcatccg
     3481 gaatatattt ctgaactgat tgaaaaactg gggggcgttg atatctgttt cggtggaatt
     3541 ggaattaatg gtcatgtagc atttaatgaa gcagatccat cccttacaaa tgaagaattt
     3601 cttgctcaga aaacccgtgt tcttaagatt acaaaagaaa caagaacagc taatgcgatt
     3661 ggtgatttta atggagctct ggaagacatg cctcattatt gtattaccat tggaattaat
     3721 gagatttcac atgcaaggaa gattcgtctg ggatgtttca gaaactggca ccgggcagta
     3781 gtacgaagag cagcatatgg agaagcaact tctgatttcc cggtatctct tcttacatca
     3841 catccggaca tcaatctgaa gatcacagag tttgttgcag cacttgattg ataagataag
     3901 taacagacaa cagaaagaag gaagaacatg agtattcgaa tcataaatgg aaaagtatat
     3961 aaaaatggag ctttttcaga agaagagatt gttctgaaag agggaaaaat cgtttctgct
     4021 caagatggag atgcagaaga agtctatgat gcaaaaggaa actatgtagt accggggttt
     4081 atagatgtac atactcatgg tggcgcaggg gtagatgtga atgcggctga tcagcaggga
     4141 ttcgaaaaga tcggacattt ttttgccaca cagggaacca catcatggct ttcatctata
     4201 ctgacagaca caaaagaaca gactttgaag accataaaag aggctgtgaa acatcaggag
     4261 gcacagaaga acggacatga aaattgtgca gatcttcttg gtattcatct ggaaggacca
     4321 ttcctttcac cggaatataa aggggctatg ccggaacatt tactgatttc tcctgatctg
     4381 gatctt
//
LOCUS       DBMDXG010000152         4376 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4794_length_4376_cov_1.929123, whole genome shotgun sequence.
ACCESSION   DBMDXG010000152 DBMDXG010000000
VERSION     DBMDXG010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_4376_cov_1.929123"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..82
                     /locus_tag="ACZPD3_05330"
     CDS             <1..82
                     /locus_tag="ACZPD3_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR1415458.1"
                     /translation="WSDGYFACSIGEVSSATIQKYIESQG"
     gene            161..1324
                     /locus_tag="ACZPD3_05335"
     CDS             161..1324
                     /locus_tag="ACZPD3_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR1415459.1"
                     /translation="MNKIYLDQASTSFPKAPSVAQAVYDYLAGSAVNVNRGGYRAAYS
                     VEEQIFETREQLLKLFHFTSGKGKNVIFTENITASLNVLLKGLLKPGDHVLVTAMEHN
                     AVMRPLVQLEKHGISFDRIPCASDGSLLLDKATALLRPETKLVVCLHASNVCGTVMPA
                     QEIGDFCKEHGLLFILDTAQTAGTINIDMEKCHIDALAFTGHKGLRGPQGTGGFLIRN
                     KLAAQIEPLISGGTGSASHSEEVPAFLPDRFEPGTPNIPGILGLHAALCDLEKQSMEE
                     IRQHELMLTQYFIDGILNLDPEEKFLRIIGKKNIENRCAVVSVQTLHMDMAQAAFELD
                     STYGIMTRVGLHCAPNAHKTLGTYPEGTLRFSFGPENTKQELDIALDALSALL"
     gene            1529..2425
                     /locus_tag="ACZPD3_05340"
     CDS             1529..2425
                     /locus_tag="ACZPD3_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HIR1415460.1"
                     /translation="MEFKQLEAFVAVVDYGSFSEAARRLYLTQPTISAHIRSLEDELH
                     MKLIIRTTKKTTITAKGYQLYDSAVRMLEIRNNLLENFTGAHKHMIDLAASTIPSSYL
                     LPELLAAFGKTHPDVYFHSIQSDSSESISRVLDGTVDLALVGQNTRDESCVFIPFCHD
                     ELVIATPVTDHYLALKDRETPAIFHDFLKDPIILREKGSGTKKEMDLFLERTGITTGN
                     LNVIARMNDLESIKKSIVNGLGISILSSRSVNDLQKTKQILVFPLEEAAHKRSFYIVY
                     SKNRILKPHVKQFVQFVRDYFM"
     gene            complement(2429..>4376)
                     /locus_tag="ACZPD3_05345"
     CDS             complement(2429..>4376)
                     /locus_tag="ACZPD3_05345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR1415461.1"
                     /translation="ANSRIASLELKDVNEPLDSYIERISQSIPQREVRTQYRKVFCVD
                     NLRECLTQGKDMIQWECPVYAAKGISLRMIRTTVEMVRNVSYDRLEALIYFSDITENY
                     FSEQIPHMLYRKNFDRIEIIDGQRDCVRLDHPGICAMEMVLAEEISYSGYVTEVMKKF
                     VPDDEKSVYEKCVRLDTIRKELQEKDRYSFSVHQFNKKGEKCLKNYTFFYLNKFFDII
                     VATVEDITEKFEQDILTGGYNRQGFIRHVERILKESEDRTGYAVVFFDIKNFKAVNEL
                     FGTEIGDMMLRKVYEDVRKSELKPLVSAREDADHFICLVERKNLDLDMLTGMCQKKLT
                     RGGKTLHLSVKCGIFMLEKKKMSVNGMIDRAKIAQRYITDEFVQSYKIYDSSMKNTYI
                     DKATLAGELEEGIEQGQFKVYFQPVVDAMTGKIASAESLIRWFHPKKGFISPGVFIPA
                     LEESGHISELDLYVLDSVNEFQKKRYQSDKITVPVSTNLSWMDFYDETIMNWLEKKCA
                     DASMPSGLCRMEITETSYAAIEADRNATLEALREAGILLLLDDFGSGYSSFGMLQNYN
                     FDIMKIDMSFVRQIETNTKTRSIIRFIIEMAHTMEIKIIAEGAETKEQVEFLRDNGCD
                     YIQGYYFYKPMPEEEFVKLLDAGR"
BASE COUNT         1225 a         1046 c          831 g         1274 t
ORIGIN      
        1 ttggtcagat ggttattttg cttgcagcat tggagaagtg tcatcagcaa ctatacaaaa
       61 atatattgag agtcaaggat aatagataac aatttacaag gctcctccca ccgcctaaag
      121 gcagtgggtt tccgcccatg acaaacgaaa ggatttattt atgaataaaa tctatctgga
      181 tcaggccagt acgtcttttc caaaggcacc atctgttgca caggcagttt atgattacct
      241 ggcaggctct gctgtgaatg tgaaccgggg aggataccgg gcagcttatt ctgtggaaga
      301 acagattttt gagacaagag aacagcttct gaagctcttt cactttactt ccggaaaggg
      361 taagaatgtt atttttacag aaaatatcac tgccagtctg aatgttcttc tgaaaggact
      421 tctgaaaccc ggcgatcatg tacttgtaac tgccatggag cataatgcag tgatgcgtcc
      481 ccttgtacag ctggagaaac atggaatttc ttttgacagg atcccatgcg cttctgacgg
      541 cagtcttctg cttgataaag cgaccgcact ccttcgtcct gagactaagc ttgtggtctg
      601 tcttcatgct tctaatgtat gcggaactgt catgccagca caggaaatcg gagatttctg
      661 caaagaacat gggcttcttt ttattctgga cactgcacag acggcaggaa ccattaacat
      721 tgatatggag aaatgtcaca tagacgcact ggcatttaca ggtcacaagg gactcagagg
      781 gccacaggga acaggtggct ttctgatccg caataaactg gcagcgcaga ttgagcctct
      841 tatcagcggt ggaacaggaa gcgcctctca ttctgaagaa gtaccggcat ttcttcctga
      901 tcgtttcgag cccggaactc ccaatatacc tgggattctg ggacttcatg ccgcactctg
      961 tgatctggag aaacagtcca tggaagaaat ccgccaacat gaattaatgc tgacacaata
     1021 ctttatagat ggtatcctga atctggatcc tgaagaaaaa tttctgcgta ttatcggtaa
     1081 aaaaaatatt gaaaaccgtt gtgcagttgt atccgtccag accttacata tggacatggc
     1141 tcaggccgcc tttgagctgg acagtactta tggaattatg acaagagtcg gccttcactg
     1201 tgcaccaaac gcacataaaa ctcttggaac atacccggag ggtactctcc gtttttcttt
     1261 cggacctgag aatacaaagc aggaactgga tattgcttta gatgcattat ccgcattact
     1321 ataacaatcc tagtgtacca acaagttgat ttcttgaaca atcttacgtc taaatttctt
     1381 atttccgcgt tgtcgtcagt cgccgtagcc accgctagcc tctctcctcc gccttgaaat
     1441 aagaaaattt atcctgcaag attgtttcaa gaatcaactt gtcggtacac tcgtacatcg
     1501 ttttcgtaca taaaaggaga ttttcacaat ggaatttaaa cagttagaag cattcgtcgc
     1561 tgtcgtggac tacggcagct tctcagaggc agcccgccgc ctctatctga cacagcctac
     1621 gatcagtgcg catatacgtt ccctggaaga tgaactgcat atgaaactga tcatccgtac
     1681 tacaaagaaa acaacgatca cagccaaagg ctaccagctt tatgacagtg cagtccgtat
     1741 gctggaaatc agaaataacc tgcttgaaaa ttttacaggt gcccacaaac atatgataga
     1801 tctggctgca tctaccattc cttcttctta tcttttgcca gaactgcttg ctgctttcgg
     1861 caaaacgcac cctgatgttt attttcactc catacagtca gacagttctg aatccatcag
     1921 ccgggtgctg gacggaactg tagatctggc tctggtaggc cagaatacac gtgatgagag
     1981 ctgcgtattt attcctttct gccatgatga gctggtcata gcaactcctg taacagatca
     2041 ctatcttgca ctcaaggaca gagaaacacc cgctattttt catgatttcc tgaaagatcc
     2101 tattatcctc agggaaaagg gctccggcac aaaaaaagaa atggaccttt ttctggagcg
     2161 cacaggcatc acaacaggca atctgaatgt gatcgcccgc atgaatgacc tggaaagcat
     2221 caaaaagtcc attgttaatg gtctgggaat ctctattctt tcatcacgct ctgtcaatga
     2281 cctgcagaag acaaagcaga ttcttgtctt tccgctggag gaagctgccc ataagcgcag
     2341 tttctatata gtctacagca aaaacaggat tctgaaaccc catgtgaagc agtttgtaca
     2401 gtttgtgcga gactatttta tgtaactgtt atctgccggc atccagcagt ttcacaaact
     2461 cttcctccgg catcggtttg taaaaataat aaccctggat ataatcacag ccgttgtctc
     2521 ttaagaactc gacctgctct ttagtttcag caccttccgc tataatcttt atctccatag
     2581 tatgtgccat ttcaatgatg aagcggataa tactgcgtgt tttggtattg gtttctatct
     2641 gccgcacaaa actcatatca attttcataa tatcaaagtt gtaattctgg agcattccaa
     2701 aggaagaata tccgcttcca aaatcatcca ggagtagcag aatccctgct tctcttaatg
     2761 cttccagagt tgcatttctg tctgcctcaa tggcagcata ggaagtttct gtaatctcca
     2821 ttctgcacag accggacggc atggaagcat ctgcacattt cttctccagc cagttcatga
     2881 tcgtctcatc atagaaatcc atccatgaca gattagttga taccggaact gtgatcttat
     2941 cggactgata acgtttcttc tgaaattcat taaccgaatc cagcacatac agatccagtt
     3001 cggaaatatg tccgctttcc tccagtgcag gaataaatac acccggcgaa ataaatccct
     3061 ttttcgggtg gaaccaccgg atcagagatt ctgctgatgc aatttttccg gtcattgcat
     3121 ccacaacagg ctgaaaatat actttgaact gtccctgctc aatcccttcc tccagttcac
     3181 ctgctaatgt tgctttatct atatatgtat tcttcatgga agaatcataa atcttatatg
     3241 actggacaaa ctcatctgta atatatctct gggcaatctt tgccctgtca atcattccat
     3301 tcacagacat ttttttcttt tcaagcataa aaatcccaca tttgacagac aaatgaagtg
     3361 tttttcctcc ccgggtaagt ttcttctgac acattccggt aagcatatcc agatcgagat
     3421 tcttacgttc taccagacag ataaaatgat ctgcgtcctc acgtgccgac accagaggct
     3481 tcagctctga tttccggaca tcttcgtaca ctttgcgcag catcatatct ccgatttctg
     3541 ttccaaaaag ttcattgact gccttgaaat tcttaatatc gaaaaataca accgcatatc
     3601 cggttctgtc ttcactttct ttcaggatcc gttccacatg acggataaaa ccctgtctgt
     3661 tatatccgcc cgtcagaata tcctgttcaa atttctccgt aatatcctca actgtagcta
     3721 caataatgtc aaaaaattta ttgaggtaaa aaaatgtata gttctttaaa catttctccc
     3781 cttttttatt aaactgatga acagagaaag aatatctatc tttctcctgc agttctttcc
     3841 gaatggtatc aagacgcaca catttttcat atacggattt ctcatcatcc ggaacaaatt
     3901 tcttcataac ttcagtaaca taaccggaat aactgatctc ttctgccaga accatctcca
     3961 tggcacagat acccggatga tccagtctca cacagtctct ctgcccgtca ataatctcta
     4021 ttctgtcaaa attctttctg tacagcatat gcggaatctg ctctgaaaaa tagttctctg
     4081 taatatcaga aaaatagatc agtgcttcca gcctgtcata agaaacgttc cggaccatct
     4141 caactgtggt tctgatcatc ctcagactga ttccttttgc ggcatacaca ggacattccc
     4201 attggatcat atcttttccc tgtgtcagac actccctcag attatctaca caaaatacct
     4261 ttctgtactg agttctcact tctctctgcg gaatagactg actgatccgc tcaatataag
     4321 aatccagtgg ttcatttaca tctttcagct ccagtgaagc aatcctgctg tttgcc
//
LOCUS       DBMDXG010000153         4368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4802_length_4368_cov_2.288493, whole genome shotgun sequence.
ACCESSION   DBMDXG010000153 DBMDXG010000000
VERSION     DBMDXG010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4802_length_4368_cov_2.288493"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="ACZPD3_05350"
     CDS             <1..809
                     /locus_tag="ACZPD3_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024491.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR1415462.1"
                     /translation="RTGKIFCLFLLIVVLVNILSPLFCRKPDETYVESLRKTEFTSET
                     AGVERICCIDDNEEALLWRLRMIGTAKESIVLSTFDLRADDNGTKILAALNCAAARGV
                     KIQLLIDGIYQQLFLAGSSDFQALASYENVEVGVYNPVTPVNLFKVNYRMHDKYLIVD
                     EKMYLLGGRNSNDIFLGDQTKGINEDRDILVYDTSEGQGESLNQLEDYFHKIWKESCV
                     SIKNGKQSSRYTDAYRHMEEIYISLLKRYNDIETCMGKGYDRSKQDHSDQ"
     gene            904..1380
                     /locus_tag="ACZPD3_05355"
     CDS             904..1380
                     /locus_tag="ACZPD3_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024491.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR1415463.1"
                     /translation="MICNGYMYDVLQGISDHAKLQIVLNAVEKGSNPWGCTDYLNQKK
                     KILETGADVYELMNDYPVHTKAVLINDRLSVVGSYNLDMRSTYLDTELMLVIDSEKLN
                     QQIHETESDYMEKSKEVLANGQETEGAKYQGKVLNRKKKLYYGVLRIIIRPLRQLL"
     gene            1601..3994
                     /locus_tag="ACZPD3_05360"
     CDS             1601..3994
                     /locus_tag="ACZPD3_05360"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR1415464.1"
                     /translation="MNLKKFVLEGNPVCRKEAVIVGDHFRITMLTTALIRFEYSEDGG
                     FEDRATQMVCNRDFPVPEFRVSDGGEELHIYTKDLEIHYDRQKFSPSGLMIRVAGGKA
                     SERVWHYGDEPKDLLGTARTLDEADGEIPLSHGIMSRNGFSVLDDSHTMAMGEDGMVE
                     PRQGNRADFYFFGYGHRYVECLQDFYRLCGKTPLLPRYTFGNWWSRYHKYTETEYKEL
                     VERFEKEEVPFSVAVVDMDWHLVEDVPPVYGSGWTGYTWNKKFFPNPPEFMDWLHKHG
                     YKITLNVHPADGVRAYEEAYPRVAEKMGIDPASKEPVLFDMTDPKFIETYFEELHHPM
                     EEEGVDFWWLDWQQGTVTKVPGLDPLWMLNHYHYLDSKWKGKRALTFSRYAGPGSHRY
                     PVGFSGDTFITWESLKFQPYFTANASNIGFGWWSHDIGGHMFGYRDDELTARWVQLGV
                     FSPMNRLHSTDNPFNGKEPWKYNQIVETVMKNFLKLRHKLVPYLYTMNRRASRAGLPL
                     VQPMYYLEPEREETYEVPNNYYFGTEMMVSPITDKLDPVTGLAGAKTWIPQGIWYDFF
                     NGRAYKGGRKVDLWRDIYEMPVLVREGSIIPLKDMEGYDNSIENPEKLEVLVYPGESG
                     EFVLWEDGGDTPEDLDENWVSTRMTKTADENGTVFIVEAAQGNTAVIPQKRSWKIRFC
                     NIQDKPQEVTVNGQVYKDAEFAEDKKLHGTIVILKDVPADAQVKVTFAADAAVYQRDY
                     AEEVYEILEKAQITYAQKTEVYKVVKELGTEAVPVLVSMNLNPSLLGVLMEILTMGI"
BASE COUNT         1368 a          781 c         1122 g         1097 t
ORIGIN      
        1 aaagaacagg aaaaatattt tgccttttcc tgctgatcgt ggtgctggta aatatacttt
       61 cacctctttt ttgcaggaaa ccggacgaaa catatgtgga aagtctgaga aagacagaat
      121 ttacatcaga gacagcaggc gtagaaagga tttgctgcat tgacgataat gaagaggcac
      181 ttttgtggag gcttcgtatg attggcactg cgaaggagag cattgtactg tctacctttg
      241 acttacgcgc ggatgataat ggcactaaaa ttcttgccgc attgaattgc gctgcggcaa
      301 gaggtgtgaa gattcagtta ttgatcgatg gaatttatca gcaactgttt ctggctggaa
      361 gcagcgattt ccaggctctg gcatcatatg agaatgtgga agtgggagtt tacaatccgg
      421 tgacaccggt caatctgttt aaggtgaatt atcgcatgca tgataaatat ctgatcgtgg
      481 atgagaaaat gtatctgctg ggcggacgca acagcaatga tatctttctg ggggatcaga
      541 caaaaggcat taatgaagac agagatatcc tggtgtatga tacatcagaa ggacagggag
      601 agtctctgaa ccagcttgag gattatttcc ataaaatatg gaaggaaagc tgtgtgagca
      661 ttaaaaacgg caaacaatct tccagatata cggatgcata ccggcacatg gaagaaattt
      721 acatatctct gctgaaaagg tacaatgata tagaaacctg catgggaaaa ggatacgata
      781 gaagcaaaca agatcactct gatcaataat ggaattgagg cgggacgaaa aacaccgcag
      841 gttctccaga caattcagta tcttactgaa aatgcagatc atgtaataat acagactcct
      901 tatgtgatct gtaatggtta catgtatgat gtattgcagg ggatttcaga tcatgcaaag
      961 cttcagatcg tgctgaatgc agtggaaaaa ggctctaatc catggggctg tacagattat
     1021 cttaatcaga aaaagaaaat tctggaaaca ggggcagatg tgtatgaact gatgaatgat
     1081 tatccggttc atacaaaagc tgtgctgata aatgaccgtc tgtcagtagt aggttcctat
     1141 aatctggata tgcgaagtac ttatctggat acggaactga tgctggtaat tgacagcgaa
     1201 aaactgaatc agcagattca cgaaacagaa tcagattata tggaaaaaag caaagaggta
     1261 cttgcaaacg gtcaggaaac cgaaggcgca aagtaccagg ggaaagtgtt gaatcggaaa
     1321 aagaagttat actacggcgt gctcaggatt atcattcgtc ctcttcggca gctgctttag
     1381 agtgtgtttg gaaaatcatt cccaaaatct cctgcaaatt gtgacgcaca tcttgcgtaa
     1441 agcatttttc agacacgttc tagaaaataa tatcacaagt atatgtcata taaatgttca
     1501 gtagctgaaa aaaggcgttg tcaatcagac tgcagaagat taaaatagac ataaaagcag
     1561 ctgttacaac tgttgttgat gtaaaggagg aaaagctttt atgaacttaa agaaatttgt
     1621 gctggaaggt aatcctgtat gcagaaagga agctgtgatc gtgggcgatc atttcaggat
     1681 tacaatgctg acaactgccc tgatccgttt tgagtacagc gaagacggag gatttgagga
     1741 cagagcaaca cagatggtat gcaatcgtga tttcccggtg ccggaattca gggtgagcga
     1801 tggcggtgag gaattacata tttacaccaa agacctggaa atccactatg acaggcagaa
     1861 attttctcca agcggtctga tgatacgtgt agcgggtgga aaagccagcg agcgcgtgtg
     1921 gcattatggt gatgaaccaa aagatctgct gggaacagcc agaaccctgg atgaagcaga
     1981 cggtgagatt ccgctgagtc atggaattat gtccagaaac ggtttttcag tgctggatga
     2041 ttcccacaca atggctatgg gcgaagatgg aatggtagag ccacgtcagg gaaacagagc
     2101 agacttttat ttctttggat atggtcacag atatgtggaa tgtctgcagg atttttacag
     2161 attatgtgga aaaacacctc tgcttccaag atatacgttc ggaaactggt ggagcagata
     2221 tcacaaatac acagagacag agtacaaaga actggtagag cgttttgaga aagaagaagt
     2281 gccgttttct gttgcagttg tagatatgga ctggcatctg gtagaggatg tacctcctgt
     2341 atatggaagc ggatggacag gatatacctg gaataagaag tttttcccaa atccgcctga
     2401 atttatggac tggctgcaca aacatggata taagatcaca ctgaacgtac atccggcaga
     2461 tggagtgcgt gcttatgaag aagcctatcc gagagttgca gagaagatgg gaattgatcc
     2521 tgcaagtaag gaaccggtac tttttgatat gacagatccg aaattcattg agacatattt
     2581 tgaggaactg catcatccga tggaagaaga gggagtagac ttctggtggc ttgactggca
     2641 gcagggaact gtaaccaagg ttccgggact ggatccgctc tggatgctga accactatca
     2701 ctatctggac agcaagtgga aaggaaaacg tgcgctgact ttctcaagat atgcaggtcc
     2761 aggaagccac agatacccgg ttggtttttc cggtgatacg ttcattacct gggagagtct
     2821 gaaattccag ccatatttca cagcaaatgc cagcaacatc ggttttggat ggtggagtca
     2881 tgatatcggt ggacatatgt ttggatacag agacgacgag ctcactgcaa ggtgggtaca
     2941 gcttggagtc ttttcaccaa tgaatcgtct gcacagcaca gataacccgt ttaatggaaa
     3001 agaaccgtgg aaatacaacc agattgtaga aacagtaatg aagaatttcc tgaaactgcg
     3061 tcataagcta gttccgtatc tttacaccat gaaccgtcgt gcaagcagag cgggactgcc
     3121 gctggtacag ccgatgtatt atctggagcc ggagcgtgag gaaacctatg aagtaccgaa
     3181 caactattat ttcggcacag agatgatggt atctccaatc acagataagc tggatccggt
     3241 aacaggactt gcaggcgcaa agacatggat tccgcaggga atctggtatg atttctttaa
     3301 cggaagagca tataagggcg gcagaaaagt ggatctctgg agagatattt atgagatgcc
     3361 ggtacttgtg cgtgaaggca gcatcattcc actgaaggac atggaaggat atgataattc
     3421 cattgagaat cctgagaaac tggaagtact tgtatatcct ggagaatcag gagaatttgt
     3481 actttgggaa gacggtgggg acacaccgga agacctggat gagaattggg tatccaccag
     3541 aatgacgaag actgcagatg agaatggaac agtatttatt gtagaagctg cacagggaaa
     3601 tacagcagtg atcccgcaga aacgttcctg gaagatccgc ttctgcaaca ttcaggacaa
     3661 accgcaggag gttactgtaa acggacaggt atataaggat gcagagttcg cagaggataa
     3721 gaagcttcat ggaacaatag tgatcctcaa ggatgtgccg gcagatgcac aggtgaaggt
     3781 tacttttgcg gcagatgcag ctgtttatca gagagattat gcagaagagg tctatgagat
     3841 tctggaaaaa gcacagatta cctatgcaca gaagacagag gtttataaag tagtgaaaga
     3901 gctgggcaca gaggcagtgc cagttctggt atccatgaat ttgaatccgt cattgcttgg
     3961 ggtactgatg gagattctta caatgggaat ttaaccgaat caagtaagtg tgccaacaag
     4021 ttgatttctc gaacaatctt gcgtctaaat ttcttatttc cgcgttgtcg tcggtcgccg
     4081 tagccaccgc tacgcctcct tcctccgcct tgaaataaga aaatttatcc tgcaagattg
     4141 tttcaagaat caacttgtcg gcacactagt tctctgcggt aacacaaaaa tcccgctcac
     4201 agttgtgtct tactgtgagc gggatttcac attttaaaag aaggagtgcc ggatcttgcg
     4261 atccggcaag tatgaaaaag aaaaagtttt attataaata aagcgtttgc tttatttgct
     4321 tattaatata cagggaaaat gtgtgcaaaa tgtgatggac tgttgaag
//
LOCUS       DBMDXG010000154         4347 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4828_length_4347_cov_1.389543, whole genome shotgun sequence.
ACCESSION   DBMDXG010000154 DBMDXG010000000
VERSION     DBMDXG010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4828_length_4347_cov_1.389543"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="ACZPD3_05365"
     CDS             complement(<1..520)
                     /locus_tag="ACZPD3_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR1415465.1"
                     /translation="MKKRQVVITGAGASGLMAAIMAARKGAAVTVLEQNEKPGKKICA
                     TGNGRCNFSNLAKPDDAYRGEHPEFAEGALVQFSVEDTIDFFREIGIYPLNRNGYLYP
                     RSNQAQSVVDVLCMEAASLGVKIKTNEQVTEIKTGTNEKNFQILTKGWHYDADALILA
                     NGSRASSVSGSDG"
     gene            complement(517..1791)
                     /locus_tag="ACZPD3_05370"
     CDS             complement(517..1791)
                     /locus_tag="ACZPD3_05370"
                     /EC_number="4.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000096 - sulfur amino acid metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR1415466.1"
                     /translation="MKEMYAQLGISSEVYDFGHKIEESLKERFEKFDKTAEYNQMKVL
                     LAMQKNKVSAECFGSSSGYGYDDIGRETLEKVYADTFHTEACLVRPQITCGTHALAIA
                     LFGNLRPGDELLAPAGKPYDTLEEVIGIRPSKGSLAEYGVSYRQVELLEDGTFDYNSI
                     RKAINEKTRLVEIQRSKGYQTRPSFSVKQIGELISFVKSIKPDVICMVDNCYGEFVDT
                     IEPSDVGADMIVGSLIKNPGGGLAPIGGYIAGTKECVENASYRMTCPGLGSEVGATLG
                     VNRSFFQGFFLAPMVTKGALKGAVFAANIYEKLGFPVIPDSTEPRQDIIQAVSLGTPE
                     GLIAFCKGIQAAAPVDSYVDPEPWDMPGYDSQVIMAAGAFVQGSSIELSADGPMKPPY
                     AVYFQGGLTWEHAKLGVLMSLQKMVDKGLVSL"
     gene            complement(1887..>2454)
                     /gene="miaA"
                     /locus_tag="ACZPD3_05375"
     CDS             complement(1887..>2454)
                     /gene="miaA"
                     /locus_tag="ACZPD3_05375"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR1415467.1"
                     /translation="LEQLAAEKGNEYLHQMLLDVDPVSAGEIHANNVKRVIRALEFYH
                     QNQSPISAHNQEQKEHETPYNLAYFVLNVPRELLYKRIDDRIDEMLKEGLLEEVQKLK
                     DMGYHRGMVSMQGLGYKEILAYLDGEYPLEEAVRILKRDTRHFAKRQLTWFRREKDAI
                     WMNKDEFDYNEDRILDEMLKVCRDRGIL"
     assembly_gap    2455..2464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2465..2841)
                     /locus_tag="ACZPD3_05380"
     CDS             complement(<2465..2841)
                     /locus_tag="ACZPD3_05380"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-dimethylallyltransferase"
                     /protein_id="HIR1415468.1"
                     /translation="MKKPLIVLTGPTAAGKTHLSIALAKAVNGEIISADSMQVYKYMD
                     IGSAKIRPEEMQGVKHYLVDELLPQEEFHIVKFQQMAKAAMEEIYAKGKIPVLVGGTG
                     FYIQAITKDIDFTQAEQEDGYRQ"
     gene            complement(2861..>4347)
                     /gene="mutL"
                     /locus_tag="ACZPD3_05385"
     CDS             complement(2861..>4347)
                     /gene="mutL"
                     /locus_tag="ACZPD3_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR1415469.1"
                     /translation="IQNKQVKLHTSGNYNVKDVIYNIYGRDITKALLEVSYENDFMKI
                     EGFVGKPEISRGNRTFENYYINGRFVKNRIIAKGIEDAYKGFLMQHKFPFVSLHIQME
                     GNDLDVNVHPSKMEVRFARGTEVYDAVYETVHKALTTREMIQTVPFGKEEPIKKQQPV
                     VKPGDVPEPFETRRRAEIPEHRPQTINTASRVSEHTALPRGTVTMAEQAVREQQIYQT
                     RAPLTKAEEQLFEGTLSDKKNEEQPMFSETKQEIAEQRTAEYLKTAVDSEGNRKQVEQ
                     NKNVISEEIRPQQLELFEEKLLAPESRSRHQLIGQIFDTYWLVQFEDKFFIIDQHAAH
                     EKVYYERFVKRFREQTVESQYLSPPLIVSLNLQEEALLKANRKYFEDFGFEIEPFGGK
                     EYCINAVPSNLYGLTEEELFLEMLDNLASEKDKDPLGIFASRLATMACKAAVKGNHQM
                     SDREANALIDELLTLENPYHCPHGRPTIISMTKTELEKKFKRIV"
BASE COUNT         1081 a         1096 c          818 g         1342 t
ORIGIN      
        1 tgccgtctga acctgaaaca gaagatgccc gggatccatt ggcaagtatc agtgcatctg
       61 catcatagtg ccatcctttt gtcaggattt ggaaattctt ttcatttgtc ccggttttta
      121 tttctgtaac ctgttcgttt gtctttattt tcacacccag actggcagcc tccatacaca
      181 gtacatctac aacactctgc gcctgattgc ttctcggata aagatagccg ttcctgttta
      241 aaggatagat tcctatctct ctgaaaaaat caatagtgtc ttcaacagaa aactgtacaa
      301 gtgcaccttc tgcaaactca ggatgttcac ctctgtatgc atcatccggt tttgcaagat
      361 tggaaaaatt gcatctgccg ttccctgtag cgcagatctt ctttccaggt ttctcattct
      421 gttccagaac agtgactgct gccccttttc gggctgccat gatcgctgcc atcaaaccac
      481 tggctcctgc tcctgtgatc actacctgcc tttttttcat aaagatacaa gccccttatc
      541 aaccattttc tgaagagaca tcaaaacgcc aagctttgca tgttcccagg taagtccgcc
      601 ctggaaatac actgcatatg gcggtttcat cggaccatct gcggaaagct cgatggaaga
      661 gccctgtaca aaagcacctg ctgccatgat aacctgactg tcatatccgg gcatatccca
      721 tggctccgga tccacataac tgtcaaccgg agctgctgcc tgaataccct tacagaacgc
      781 gatcagacct tccggtgtgc caaggcttac tgcctgaatg atgtcctgac gtggctctgt
      841 ggaatccggg attaccggga atccaagttt ctcataaata tttgccgcaa acacagctcc
      901 ctttaacgca cccttggtaa ccattggtgc aaggaaaaat ccctggaaga aagaacggtt
      961 gactcccaga gttgcgccta cctcactgcc aagtccggga caggtcattc tgtaagaagc
     1021 attctccacg cactccttag tacctgcaat ataaccgccg ataggcgcaa gtccgccgcc
     1081 cggatttttg atcagagaac caacaatcat atcagctccc acatcacttg gctcaatggt
     1141 atctacaaac tcaccgtagc agttatccac catacagatc acatccggtt taatactctt
     1201 tacaaaagaa atcagttctc caatctgctt tacagagaaa gacggacgtg tctggtatcc
     1261 tttggaacgc tggatctcca caagacgtgt cttttcattg attgctttac ggatgctgtt
     1321 ataatcaaag gttccgtctt ctaacagttc cacctgacgg taacttactc catattctgc
     1381 gagagaacct tttgacgggc ggatgccgat tacctcttcc agagtatcgt aaggctttcc
     1441 ggcaggtgcc agaagttcat cacccggacg gagattaccg aataaggcaa tggccagcgc
     1501 gtgagttccg catgtgatct gcgggcgtac cagacaggct tccgtatgga aagtatctgc
     1561 ataaaccttt tcaagtgttt cccggccaat atcgtcatat ccataaccgg aagaacttcc
     1621 gaagcattcg gcacttacct tatttttctg catggcaaga agcaccttca tctggttata
     1681 ttctgccgtt ttatcaaatt tttcaaatct ttcttttaaa gattcctcaa tcttatgacc
     1741 aaagtcataa acctctgaag aaatcccaag ctgtgcgtac atttctttca taatttgttt
     1801 cgtcctcatt tctgtttgca ttattatctt tattttatct ctgaatttca cttattctgt
     1861 atcagtgatt tttaagaaaa attcggttat aaaattcctc tgtccctgca taccttcagc
     1921 atttcatcaa ggattctgtc ttcattatag tcaaattcat ccttattcat ccagatggca
     1981 tccttctccc tgcgaaacca ggtcagctgg cgttttgcaa aatgtctggt atctcttttc
     2041 aggatcctga ccgcttcctc taacggatac tcgccatcca gatatgcaag gatttcttta
     2101 tatccaagtc cctgcatgga aaccatcccc ctgtggtatc ccatatcttt taacttctgc
     2161 acttcctcaa ggagtccctc ttttaacatc tcatcaatac ggtcatcaat ccgtttataa
     2221 agaagttccc ttggtacatt cagcacaaaa tatgccagat tataaggggt ttcgtgttcc
     2281 ttctgctcct gattatgtgc ggagatcggc gactggttct gatgataaaa ctccagtgcc
     2341 ctgatcacac gttttacatt attggcgtgg atttccccgg cactgacagg atcaacatcc
     2401 aaaagcatct ggtgcagata ttcatttccc ttctctgcag caagctgttc cagcnnnnnn
     2461 nnnntcctga cggtatccgt cttcctgctc tgcctgagta aaatcaatat cctttgtaat
     2521 agcctgtata taaaatccgg ttcctcctac cagaacagga attttcccct tggcataaat
     2581 ttcttccatg gcagcttttg ccatctgctg gaatttcacg atatgaaatt cttcctgagg
     2641 cagcagttca tctaccagat aatgctttac tccctgcatc tcctccggcc ggatcttagc
     2701 tgatccaata tccatatatt tgtagacctg catggaatca gcagaaatga tctctccgtt
     2761 tactgctttc gcaagagcaa tggatagatg ggtttttcct gctgccgtcg gtcctgtaag
     2821 aacgatcagt ggctttttca taatagctcc tttatataaa ctatacaata cgtttaaatt
     2881 tcttttccag ttctgtttta gtcatggaaa taatcgtcgg tcttccatga ggacagtgat
     2941 aggggttctc cagtgtgagc agttcatcga tcaaggcatt tgcttcccgg tcggacatct
     3001 ggtgatttcc ctttaccgca gccttacatg ccatggtggc cagtctggaa gcaaagattc
     3061 caagtggatc cttatccttc tcagaagcaa gattgtcaag catttccaga aaaagttctt
     3121 cctccgtaag accgtacaga ttggacggga ctgcattgat acagtattcc ttaccgccaa
     3181 aaggttcaat ctcaaatcca aaatcctcga aatacttacg gttagcctta agaagagctt
     3241 cttcctgaag attcagggat acgattagcg gcgggctgag gtactgggat tctactgtct
     3301 gctcacgaaa cctttttacg aaacgttcat aataaacctt ttcatgtgcc gcatgctggt
     3361 cgataataaa aaatttatcc tcaaactgaa caagccagta agtatcaaaa atctgtccga
     3421 tcagctgatg acggcttcgg gattcaggag caagaagttt ctcctcaaat aattccagct
     3481 gctgtggtct gatttcttct gatattacat ttttgttttg ttctacctgt tttctgtttc
     3541 cttctgaatc cacagcagtc ttcagatatt ctgcagttct ctgttctgca atttcctgct
     3601 ttgtctctga aaacatcggt tgttcttcat tctttttatc gcttagtgtt ccctcaaaaa
     3661 gctgttcctc tgccttggta agaggagccc tggtctgata gatctgctgt tcacgcactg
     3721 cctgttctgc cattgtaaca gttccccgtg gtagtgcggt atgctcagaa accctggaag
     3781 ctgtatttat agtctgtgga cgatgctccg gaatttcagc ccgacgtctg gtttcaaatg
     3841 gctccggtac atcccccggt tttaccacag gctgctgctt tttaatcggt tcttcttttc
     3901 caaaaggcac tgtctggatc atttctcttg tggtaagagc cttgtggact gtctcgtaaa
     3961 ctgcatcata aacttctgtt cctcttgcaa accgcacctc catcttgctt ggatgcacat
     4021 ttacatccag atcatttccc tccatctgaa tatgaagaga aacaaaggga aacttatgct
     4081 gcatgagaaa acccttatag gcatcctcta tcccctttgc aatgatccgg ttctttacaa
     4141 aacgtccgtt aatataataa ttctcaaagg tacggttacc tctggaaatc tccggttttc
     4201 ccacaaaacc ctcaatcttc atgaaatcat tctcgtagga tacctcaaga agtgctttgg
     4261 tgatatctct tccatatata ttatagatca catctttcac attgtaattt ccggaagtat
     4321 gaagcttcac ctgtttattc tggatat
//
LOCUS       DBMDXG010000155         4335 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4840_length_4335_cov_1.393245, whole genome shotgun sequence.
ACCESSION   DBMDXG010000155 DBMDXG010000000
VERSION     DBMDXG010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4840_length_4335_cov_1.393245"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2694
                     /locus_tag="ACZPD3_05390"
     CDS             <1..2694
                     /locus_tag="ACZPD3_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HIR1415470.1"
                     /translation="TQKSVLLANTEFKVYDLDNQKYVEQTTSYPKPTVHKSYFTNEEG
                     YLVLPKNLKPGNYRIEEVTAPDGYTISKNYVTVAVDTDTAYLTDPVTGDAVIDVEYTN
                     APVKGQLKIYKQGEVLKGFDKDFQYEMAGLAGAEFEVYAAEDIYTADHQVDADGQRTL
                     YFAKDALVATVTTDTDGYATVKNLPLGRYYVKEKNAPDTYVLNTVPENVEFAYADQDT
                     AVIEKEISVTDERQKVSITVEKQDAETGNTVAGAVFGIYNAKDIQTKDGKVIVKADTL
                     LQEMTSDEKGQAACTLDLPLGSYYVKELKAPDGFVSSDEVLRFDASYQGQDVQTVTLK
                     SVKKNQPTTVEITKSDATTGVELDGAYLKVTDKDGNVVDSWTSSKDAPHVIKYLKVGE
                     TYTLHEEFAPHGYLVANDVTFTVKDTGEVQKVEMKDEVPVGGLIINKKGEFLDSVTLA
                     DKVKGVVEHIFNYVTGKLTDVTFEVYAEEDIKAADGVSDDYYKKDELIATIKTDETGI
                     AKLENLPLGKYYVKETGTAYGHVLDGEIRHVDLTYVDQNTPVVVYDKDWQNNRQRVEV
                     NVLKKEKDSDRTLEGAIFGLFAKEDIKSETTGKVLIEADEIIELKSTDEEGKITFVAD
                     LPIGATYYVKELYAPDGFVTNDEVKEFTFEYAGEDQPTVTYDFTFDNQPTVVEFTKSS
                     LTTGKELPGCKLKVVDADGNIVDEWTSGKEAHVIRELTVGKEYTLVETKPADGYVTAE
                     SVKFTIKDTADVQKVEMKDDVTKVKISKQDIAGKELPGAKLTILDQEGKVVESWTSTE
                     KAHYIEMLPIGKYTLREETAPDGYLVAKDVEFEVKDTCEVQHVTMVDEKKPAEKTPEG
                     GKPVSDSPKTGDNTNLWLWFMLLGIGATGTGALAFMRKKKH"
     gene            2751..2906
                     /locus_tag="ACZPD3_05395"
     CDS             2751..2906
                     /locus_tag="ACZPD3_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415471.1"
                     /translation="MGETMRNIIIFVALLGAAAIFTVLYCLIAAGSQAEKKLTEIMRK
                     EEEQNED"
     gene            2896..3174
                     /locus_tag="ACZPD3_05400"
     CDS             2896..3174
                     /locus_tag="ACZPD3_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415472.1"
                     /translation="MKTNKDLIIEPNAAIEGRKYSEEKPWSCKYCYWWGGKKKGCSLK
                     QCHYLLPEKKKAVKTTPCGGCPYGKHVPCIGYCIARLQSEMKGKKGRG"
     gene            3175..3738
                     /locus_tag="ACZPD3_05405"
     CDS             3175..3738
                     /locus_tag="ACZPD3_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HIR1415473.1"
                     /translation="MQEEVNQKVISLSIQGVKITASVLKAALRKFLEMDNRRKQKATQ
                     VKMAEKTGRAQEKGREKARKKIEKKKPHGKQTIKQLNAQGVQLSNIKITDENIKSFDR
                     VARKYGIDYSLKKEVGADPPKYLVFFKAKDVDVMTAAFREYAGVELKKKQAKKPSVRK
                     KLQKSVERKAKHRQRVKQRQKSRGQER"
     gene            3735..>4335
                     /locus_tag="ACZPD3_05410"
     CDS             3735..>4335
                     /locus_tag="ACZPD3_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HIR1415474.1"
                     /translation="MIREKLQKIQVKRLVILNLPYFFIFYVADKGSWLYRHCLGESMV
                     QRLGVMLVNFRLAFLSWLPSIALQDLTVGVLVAGALKLVVYYRSKNAKKFRQGVEYGS
                     ARWGNRKDIEPFMDPVFENNVILTETERLTMNSRPKAPKYARNKNVIVIGGSGSGKTR
                     FYVKPNLMQMTDHVSYVVTDPKGTIIVECGKMLVNGGYRI"
BASE COUNT         1494 a          791 c         1087 g          963 t
ORIGIN      
        1 acacagaaat ctgtactcct ggcaaataca gaattcaagg tatatgacct ggataaccag
       61 aaatatgtgg aacagacaac atcctatccg aagccaacgg tacacaaatc ctatttcaca
      121 aatgaggaag gatacctggt actgccaaag aatctgaaac ctggcaacta ccggattgag
      181 gaagtgacgg cacccgatgg gtacacaata tccaaaaact atgtgacagt tgcagttgat
      241 acagatactg cttatctgac cgatcctgtc acaggagatg cagtcattga tgtggaatat
      301 accaatgccc cggtcaaagg tcagttaaag atctataaac agggtgaagt gttaaaggga
      361 tttgataagg acttccagta tgaaatggct ggacttgcag gtgctgaatt tgaagtctat
      421 gcggcagagg atatctatac tgccgatcat caggtagatg cagatggaca gagaactttg
      481 tactttgcaa aggatgcact ggtggcaacc gttacaacgg atacagatgg ttatgcaacc
      541 gtaaagaatc ttccacttgg cagatactat gtcaaagaga agaatgcacc ggacacttat
      601 gtgttaaata cggtgccgga aaatgtcgaa tttgcttatg cagatcagga taccgcagtg
      661 atcgagaaag aaatctctgt cacagatgaa cgacagaaag tgtcgattac ggtcgaaaaa
      721 caggatgctg agacaggcaa tacagtagcc ggagcagtgt tcggtattta caatgcaaaa
      781 gatattcaga cgaaagatgg aaaagtgatc gtcaaagcag atactctttt acaggaaatg
      841 acttctgatg aaaaaggtca ggcagcatgt acactggatc ttccacttgg aagctattat
      901 gtaaaagaac tgaaagcacc agatggattc gtatcttcgg atgaagtact gagatttgat
      961 gcttcttatc aggggcagga tgtacagaca gtaacattaa aatccgtaaa gaagaaccag
     1021 ccgacaactg ttgagatcac aaaatcagat gcaacaaccg gtgtagaact ggatggagct
     1081 tatctgaaag taacggataa agatggaaat gtagtagaca gctggacatc ttccaaagat
     1141 gcaccgcatg tgatcaaata tctgaaagtg ggtgagactt ataccctaca tgaagagttt
     1201 gcaccgcatg gttatctggt agccaatgat gtgacattca cagtcaaaga taccggagag
     1261 gttcagaaag tggaaatgaa ggacgaggta ccggttggtg gactcatcat caacaaaaag
     1321 ggagaatttc tggattctgt tacgctggcc gataaggtca aaggtgtggt ggaacacatt
     1381 ttcaactatg taaccggaaa actgacagat gttacgtttg aagtctatgc tgaggaagac
     1441 attaaggctg ccgatggcgt cagtgacgat tactataaaa aggatgaact gatcgcaacg
     1501 atcaagacag atgaaaccgg tattgcaaaa ctggaaaatc ttccacttgg taaatactat
     1561 gtgaaagaaa caggaactgc ttacggtcat gtactggatg gtgagatccg tcatgtagat
     1621 ctgacttacg tggatcagaa tacaccagtt gtagtctatg acaaagactg gcagaacaac
     1681 cgccagagag tagaagtcaa tgtacttaag aaagaaaaag attctgaccg tacattggaa
     1741 ggcgctatct ttggattatt tgccaaagaa gacattaagt ctgagacaac aggtaaagtc
     1801 ctgatcgaag ccgatgagat cattgagctg aaatctacgg atgaagaagg aaagatcaca
     1861 tttgttgcag atcttccaat aggagcaact tactatgtaa aagagctcta tgcaccggac
     1921 ggattcgtaa ccaacgatga agtaaaggaa tttacctttg agtatgcagg tgaagatcag
     1981 ccgacagtca cttatgattt tacctttgat aatcagccaa ccgtcgttga atttaccaaa
     2041 tccagtctga caaccggaaa agaacttcca ggatgtaagc tgaaagtggt agatgctgat
     2101 ggaaatatcg tggatgaatg gacttccggt aaagaagcac atgtgatccg tgaactgact
     2161 gtcggaaaag aatacacact ggttgaaaca aaacctgctg atggttacgt gacagctgaa
     2221 agcgtaaagt tcacgatcaa agatactgcc gatgtgcaga aagttgagat gaaggatgat
     2281 gtaacgaagg tgaaaatctc caaacaggat atcgccggaa aagaacttcc gggagcgaaa
     2341 ctcacaatcc ttgatcagga aggaaaagtt gtggaaagct ggaccagtac agagaaagca
     2401 cattacatcg aaatgcttcc gatcggaaaa tacactctcc gtgaagaaac ggcaccggat
     2461 ggctatctgg ttgcaaaaga tgtggaattc gaagtaaagg atacctgtga agtacagcat
     2521 gtaactatgg tggatgagaa aaaaccggca gaaaagacac cggaaggtgg aaaacctgtc
     2581 agtgattcac caaagactgg ggataacacc aatctgtggc tgtggtttat gcttctcggc
     2641 attggtgcga ctggtacagg agcccttgca tttatgagaa aaaagaaaca ctaattaaat
     2701 aagaatgagc gttcatggaa accgaaagga atctgtggac gcttttttag atgggagaaa
     2761 ccatgagaaa tatcattatt tttgttgctc ttctgggagc agcggcaata tttacagttt
     2821 tgtattgtct cattgctgcc ggaagtcagg cagagaaaaa actgacagaa atcatgagaa
     2881 aagaggaaga acagaatgaa gactaataaa gatctgatca tagaaccaaa tgcagctatt
     2941 gagggaagaa agtacagcga agaaaagcca tggtcctgca agtactgtta ctggtgggga
     3001 ggcaaaaaga aaggctgtag cttgaaacag tgccactatc tgcttccgga aaagaaaaaa
     3061 gcagtaaaaa caacaccatg tggtggctgt ccgtatggaa aacatgttcc atgcatcgga
     3121 tattgtattg cccgtcttca gtctgaaatg aaaggaaaga aaggacgtgg gtaattgcag
     3181 gaagaagtaa atcagaaagt gatcagcctg agcatccagg gagtcaagat cactgccagt
     3241 gtgctaaaag cagcccttcg gaagttcctg gaaatggaca accggaggaa acagaaagca
     3301 acgcaggtga aaatggctga aaagaccgga cgggctcagg agaaaggaag ggaaaaagcc
     3361 cgaaaaaaga ttgaaaagaa aaagccacat ggaaagcaga ccatcaagca actgaatgca
     3421 cagggagtac agctttccaa cataaagatc acagatgaaa atatcaaatc ctttgaccgt
     3481 gtggcaagaa agtacgggat tgattacagc ctgaaaaaag aagtcggtgc tgatccacca
     3541 aaatatctgg tcttctttaa ggctaaagat gtggatgtga tgacagcggc tttccgggaa
     3601 tatgccggag tagaactgaa aaagaaacag gcgaagaaac catccgtcag aaagaaactg
     3661 cagaaatctg tagaaagaaa agcaaagcac cgccagcgtg tgaagcagag acaaaaatcc
     3721 agaggtcagg aacgatgatc cgggaaaaat tacagaagat ccaggtcaaa cgactggtga
     3781 tcttaaatct cccatatttc tttatcttct atgtagcaga taaaggatcc tggctgtacc
     3841 ggcattgcct gggagaaagc atggtccaga gacttggcgt gatgttagtc aatttccgac
     3901 tggcattttt aagctggctg ccaagtatcg ctttgcagga tcttacagta ggtgttctgg
     3961 ttgcaggtgc attaaaactg gtggtctact atcgttctaa aaatgcaaag aagttccgac
     4021 aaggtgtgga gtatggatct gcaagatggg gaaacagaaa agacatcgaa ccctttatgg
     4081 atccggtctt tgaaaacaat gtcatcctca cagaaacgga acgtctgacc atgaacagcc
     4141 ggccaaaagc cccaaagtat gccagaaata agaatgtgat cgtcatcggt ggttccggat
     4201 ccggaaagac aagattctat gtaaaaccca atcttatgca gatgacggat cacgtgtcct
     4261 atgtggtcac tgatccgaag ggaacgatca tcgttgaatg cgggaagatg ctggtaaatg
     4321 gaggataccg gatca
//
LOCUS       DBMDXG010000156         4267 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4926_length_4267_cov_1.371339, whole genome shotgun sequence.
ACCESSION   DBMDXG010000156 DBMDXG010000000
VERSION     DBMDXG010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_4267_cov_1.371339"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="ACZPD3_05415"
     CDS             <1..850
                     /locus_tag="ACZPD3_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415475.1"
                     /translation="CISIGLAPAFKMKFWNIGAEGQILIGGACSAAVMIYAGDKMPTG
                     LLLIVMFVASALGGMIWGMIPAVFKANWNTNETLFTLMLNYVAMQVVTYCIVFWENPK
                     GSNTVGIINQATKGGWLPELFGQKYGWNVVIVLILTVGMFIYLKYCKQGYEIAVVGES
                     ENTARYAGIQVKKVIIRTMAISGAICGIAGFVIVSGASHTISTATAGGRGFTAIIVAW
                     LSKFNTFIMVAVSFGIVFMNQGAVQIATQYGLNENASNVLLGIILFFLIGAEFFINYR
                     VKRAKK"
     gene            863..1843
                     /locus_tag="ACZPD3_05420"
     CDS             863..1843
                     /locus_tag="ACZPD3_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415476.1"
                     /translation="MSVLIVFIQKAIVQGICILYGALGEIMTEKSGNLNLGIPGIMYM
                     GGISGLMGAFLYEKDNPDPNALVGVLISFLCAFACAAIGGLIYSILTITLRVNQNVTG
                     LALTIFGTGFGNFFGGSISKLAGGVGQISVKVTGSAYTAKIPGLSKIPVIGGILFNYG
                     FLTYLCIIIAVLLSFFLFKTRAGLNLRAIGENPGTADAAGINVIKYKYLSTCIGAGLA
                     GLGGLYFVMEYSGGTWTDNGFGDRGWLAVALVIFALWKPLNAIWGAFLFGALYILYLY
                     IPGLGRSMQEVFKALPYVVTIIVLVFTSFRKKKEHQPPAALGLPYFREER"
     gene            1944..2144
                     /locus_tag="ACZPD3_05425"
     CDS             1944..2144
                     /locus_tag="ACZPD3_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR1415477.1"
                     /translation="MAKVSKDMLIGQLLQIDANIAPILMRAGMHCLGCPSSQMESLEE
                     AAMVHGLDVDVLVNQINDFLGE"
     gene            complement(2327..3073)
                     /locus_tag="ACZPD3_05430"
     CDS             complement(2327..3073)
                     /locus_tag="ACZPD3_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIR1415478.1"
                     /translation="MKIEKINDNQIRCTLTREDLENHQIRISELAYGTEKAKKLFRDM
                     MQQAQIQFGFEADNIPLMIEAIPVSTESIILIITKVEDPEELDTRFSKFAPFRGNDKT
                     DTLQLDGADNIIDIFQKLYEAKMKSTQTKDGKSAQNTGAKENDDTQVPDVNLIRLYEF
                     DTLDDVIAAAHGLNGYFTGTNTLYKDPADELYKLVLHQSSLSPEDFNRVCNILTEYGQ
                     GKAFSLSGEAYLTEHGELISDSALQQLIQL"
     gene            3771..3971
                     /locus_tag="ACZPD3_05435"
     CDS             3771..3971
                     /locus_tag="ACZPD3_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415479.1"
                     /translation="MRKPLEKELKSYREQGISLYLNGILSNPKTIAKACQIAEGGGYM
                     RDYVEDEKGRIARVDFDFVKEK"
BASE COUNT         1257 a          758 c          948 g         1304 t
ORIGIN      
        1 gtgtatttcc attggtctgg cacctgcatt taaaatgaaa ttctggaaca tcggtgcaga
       61 aggtcagatc ctgatcggtg gtgcctgctc tgctgccgtc atgatctatg caggagataa
      121 gatgcctaca gggttacttc tgatcgtaat gtttgtggca agtgcacttg gcggaatgat
      181 ctggggaatg attccggctg tatttaaagc aaactggaat acaaatgaga cactgtttac
      241 tctgatgttg aattatgttg ccatgcaggt agtaacttat tgtattgtat tctgggaaaa
      301 tccgaaaggt tccaatacag tcggtatcat caatcaggct acaaaaggcg gatggcttcc
      361 ggaattgttc ggacagaaat atggctggaa tgtagtcatt gtcctgattc tgacagtggg
      421 aatgtttatt tatctgaaat actgtaagca gggatatgag atcgcagtag taggtgagag
      481 cgagaatact gccagatatg ccggtatcca ggtgaaaaag gttattatcc gtacaatggc
      541 aatctccggt gcaatctgtg gtatcgcagg ttttgttatt gtcagcggag ccagccatac
      601 gatttccact gcaacagcag gcggtcgtgg atttactgcg atcattgtcg catggctttc
      661 caagtttaat acatttatta tggttgcagt ttcctttggc atcgtattta tgaaccaggg
      721 tgccgtacag atcgcaacac agtatggact gaatgagaat gcttccaatg ttcttctggg
      781 aatcattctg tttttcctga tcggtgcaga attctttatc aactacagag taaagcgtgc
      841 aaagaaatag gaggtagcgg caatgagtgt actgatagtt tttattcaaa aagcaattgt
      901 ccagggcatc tgtattctgt atggggcgct tggtgagatt atgacagaga aatccggtaa
      961 tctgaatctt ggtattccgg gaattatgta tatgggcggc atttcaggtt tgatgggagc
     1021 tttcctctat gagaaggata atcctgatcc caatgcactt gtgggtgtac tgatatcgtt
     1081 tttgtgtgcg tttgcatgtg ctgcaatcgg tggtctgatc tacagtatcc tgactatcac
     1141 acttcgtgta aatcagaatg taacaggtct tgcacttacc atttttggta caggttttgg
     1201 taacttcttt ggcggttcta tttccaagct tgcaggcggt gtaggacaga tttccgtaaa
     1261 agtaacagga agtgcctata cggcaaaaat cccgggattg tctaagattc cggttatcgg
     1321 aggtatttta tttaattatg gtttccttac atatctctgt attattatag cagtcctgtt
     1381 atcgtttttt ctgttcaaga caagagcggg attgaatctc agggcaatcg gtgaaaatcc
     1441 gggaactgca gatgcagcag gtatcaatgt tatcaaatat aagtatctgt caacctgtat
     1501 cggtgcaggc ctggcaggtc tgggcggact ctatttcgta atggaatatt caggaggaac
     1561 ctggacagac aatggttttg gagatcgtgg ctggcttgct gttgcactgg taatctttgc
     1621 attatggaaa ccactgaatg caatctgggg agcattcctg ttcggagctc tttatattct
     1681 gtatctttat atcccgggac ttggacgaag catgcaggaa gtatttaaag cactgcctta
     1741 tgtggtaacg attatcgtac tggtatttac aagcttccga aagaaaaagg aacaccagcc
     1801 gcctgctgcg ctgggacttc cgtatttcag ggaggaacga taaaattaaa agtttgcagt
     1861 tgattccaga gtaaaatagt attataatat cagaagctat aaaagctgag tagtatgaag
     1921 aaaaattaag gaggaaatat acaatggcta aagtatcaaa agacatgctg atcggtcagc
     1981 ttcttcagat tgatgcaaat atcgcaccga ttcttatgag agcaggaatg cattgccttg
     2041 gatgcccatc ttcccagatg gaatctctcg aagaagcagc tatggttcat ggactggatg
     2101 ttgatgtact ggttaaccag atcaatgatt ttcttggaga ataatcttcc tgaaaggtaa
     2161 tcatatgacg gactatgaca attgttgaat tgtcatagtc tgacatgtaa aaaggatgtt
     2221 ctgaataagt tcaggacatc ctttttttga gaaatctgta atttactatg gtataagaaa
     2281 agagctttca gatgaaaact gaatgctctt ttaattatga aaattattac agttgaatca
     2341 gctgctgaag ggcagaatcg gaaatcagtt ctccatgttc tgtcagataa gcttctcctg
     2401 aaagagagaa agcctttccc tgtccatatt ccgtaagaat attacatacc ctgttaaaat
     2461 cctccggaga aagtgatgac tggtgaagta ccagtttgta aagctcatcg gccggatctt
     2521 tgtacagggt gtttgtaccg gtaaaatagc cgttcagtcc atgggcggcc gcaatcacat
     2581 cgtccagtgt gtcgaactca tagagacgga taagatttac atcaggaacc tgagtatcgt
     2641 cattttcctt tgctccggta ttctgagcag atttgccatc ttttgtctga gtactcttca
     2701 tctttgcttc gtaaagcttc tggaagatat caataatatt gtctgcaccg tcaagctgaa
     2761 gagtatctgt cttatcattc cctctgaatg gagcaaattt ggagaaacgg gtatccagtt
     2821 cttcaggatc ctctactttg gtaatgatca ggatgatgct ctcggtactc accgggatgg
     2881 cctcgatcat taatggaata ttatctgcct caaaaccaaa ctgtatctgt gcctgctgca
     2941 tcatatcgcg gaaaagtttc ttggctttct cagtgccgta agccagttca ctgatacgga
     3001 tctgatggtt ctccaggtct tctcttgtca gtgtgcagcg tatctggttg tcgttgatct
     3061 tttctatctt catgttgtca catcctattc tgaataacat tgaattatct ggcagcttca
     3121 taattgcctg tgaaaatcca ttaaaagtgt ttctgataat gaaaatttaa cttttgaaag
     3181 ctgcggacaa ttacggataa attaaaagtt actattgttt ttttgaacag caattttaaa
     3241 ggattgccat agatgcgctg tctttggaaa atcgctgcaa gtaacttcaa aaattctatt
     3301 gtctgactgt gaatttataa aattttatac actatattat ataacacaga actgcaaaat
     3361 gtaaagataa gtaatcaacc aaaatattaa atttttagaa acaaaacaga aaaaaataca
     3421 taaatgaaga aaaacacttg catctgtttg ccagatggat ataatgaaaa cagaagaagc
     3481 agtaaacctg cggttcacat aagcgaagat ctgaaacatt aaccaaagtg atcttttatt
     3541 tctaaacttc attgccgttt ttcatgcagc gttcctgctg tggcaatgta caggtaaaat
     3601 ttccatgatg ataagaagtt gaaacgttac tgttcactcc gttcacagta acgataaaac
     3661 aggtgcaggg atattgtttt ttcaacagcc gataaggtgc tttctaaaaa atcttatgaa
     3721 agaagggagg taagcttacg gaaaatatat cacgggcaac caaaaagagc atgcgtaaac
     3781 cactggaaaa agaattgaag agttatcgtg aacagggaat ttctctgtat ctgaatggta
     3841 tactcagtaa tccgaaaaca attgcaaaag cctgtcagat tgcagaaggt ggtggataca
     3901 tgcgtgatta cgttgaagat gaaaaaggac ggatcgctcg tgtggatttt gatttcgtca
     3961 aagaaaaata acaaaagaga aagctgcccg aacagattct gaatcagtcc ccccgatgtt
     4021 cataatgcct tcgggcagca taagaatata attacgcata tcttaaaaat ttgtgaaaaa
     4081 tacgattttg caatacaatt gtaatatttt atggtatact taagccagta tcatcaagtt
     4141 gccgatacag ttgataatac tggtacatca tactcaaaat aactatatga aatacctttc
     4201 tgtgagctga tgtatcaata gcgatcgtgt tactgtctgc tttgtttccg gtaacacaat
     4261 atttact
//
LOCUS       DBMDXG010000157         4264 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4929_length_4264_cov_2.354312, whole genome shotgun sequence.
ACCESSION   DBMDXG010000157 DBMDXG010000000
VERSION     DBMDXG010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4929_length_4264_cov_2.354312"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..266)
                     /locus_tag="ACZPD3_05440"
     CDS             complement(156..266)
                     /locus_tag="ACZPD3_05440"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415480.1"
                     /translation="MVSVTRYDALIRTKSPINCDAHLVESIFQTHSRNSD"
     gene            240..425
                     /locus_tag="ACZPD3_05445"
                     /pseudo
     CDS             240..425
                     /locus_tag="ACZPD3_05445"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            complement(537..995)
                     /locus_tag="ACZPD3_05450"
     CDS             complement(537..995)
                     /locus_tag="ACZPD3_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR1415481.1"
                     /translation="MILKEKERSVIEDLQTQEKSCVEKYGKYAEQARDPELKNLFRNI
                     QQEEQKHYESLSMILSGSVPECNCNDRSGKDYQPKAVYTAMSSPEDKEHDAFLATDCI
                     GTEKLVSGTYNDDVFAFGDSGVRKLLADIQIEEQNHAEMLYKYKTVNGMA"
     gene            1354..2721
                     /locus_tag="ACZPD3_05455"
     CDS             1354..2721
                     /locus_tag="ACZPD3_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415482.1"
                     /translation="MKRIDFENGTVTNNILSAALPMLVAQILNLLYNIVDRIYIARIH
                     DIGTTALGAVGLCFPIIMIITAFSNLFGSGGAPIFSINRGKGDSRTADMIMNTAFTML
                     CGSAAVLMLIGFLFARPLLTLFGASDDALVYAYPYLMIYLLGTLPSMIATGMNPFINA
                     QGYAIIGMLSVTVGAVANIILDPIFIFVLDMGIKGAAIATVISQILSALLVFYFLHGK
                     SELKVRWIHINEISECTRHARDIISLGSAGFIMQLTNSLVSICCNNVLSVTGGDIYIS
                     VMTIISSVRQMVETPIHAINEGTSPVLSYNYGARRPDRVKKAGVVLIIMVLIYTGIMW
                     SVILISPEFLIGIFSSDKLLLKDAVPALKLYFAAFIFMDLQYIGQTVFKSLNKKKQAI
                     FFSLLRKVFIVVPLTYFLPYGLHMGTDGVFMAEPVSNVIGGSLCFITMLVTILPELNK
                     MGNSR"
     gene            complement(2745..3173)
                     /locus_tag="ACZPD3_05460"
     CDS             complement(2745..3173)
                     /locus_tag="ACZPD3_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR1415483.1"
                     /translation="MSDNKNVNQDKGLQGNEKIEQAIAALQQEATQEMLAHTLTVIRR
                     RMRENGQFILSVEPPTGDNQLRIGTVKTGDGKVWWAAFTSFEEELKGGGSVQSTFLTD
                     IDQLFNSALQVNEIEGIILNPWNRTIMLDKNLINIILGNV"
     gene            complement(3278..3589)
                     /locus_tag="ACZPD3_05465"
     CDS             complement(3278..3589)
                     /locus_tag="ACZPD3_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HIR1415484.1"
                     /translation="MKLLSIEYIPGVEFEALGIVKGTVVQTKNVGKDFMAGMKTLVGG
                     EITGYTEMLNEARQIATKRMVDEAKEMNADAVIGVKYGSSQVMSGAAEVIAYGTAVKY
                     K"
     gene            complement(3570..4211)
                     /locus_tag="ACZPD3_05470"
     CDS             complement(3570..4211)
                     /locus_tag="ACZPD3_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1415485.1"
                     /translation="MKIKQVEELVGITRKNIRFYEEQGLLNVERAENGYREYHTADIA
                     RLQEIKLFRKMDISIEEMRALFEKRKSLQFCLEQHLGELERRREGLVKMQEMCERLIA
                     EHQSLDTLNAENCLEEIEQMEKEGARFMDIKKTDIRKKRRTGAIIGAVVMILLMGFTI
                     GLMLWANTQDPIPTGLLIFLIAIPVVIIGGILAALAGRMKEIEGGEEDEASKY"
BASE COUNT         1078 a          979 c          780 g         1427 t
ORIGIN      
        1 atattatcag acccaaaaaa tccaatacaa atactccaac aaatataaat aatatactac
       61 taatgagtag tgtattatat ttactatatt cattcaagta tttataaaaa attcttattc
      121 attcctgcca aaattcacta ttacacctta cgtaactaat cggaattcct agaatgtgtt
      181 tgaaaaatgc tttccacaag atgtgcgtca caatttatgg gagattttgt tcgaataagg
      241 gcgtcgtagc gggttacgga aaccaaattc ggacaaaata tatcgcaaag tggggcgtgc
      301 agattatggg aatgattttt caaacacgct ctagcaatta tcttatggct tttatcatga
      361 caaattttac aaaatttttc ataaaatatc ccaacattta taccatcatg ttttttataa
      421 aataagatgt acctggcaag agacagtcaa aaccaattgt ctcctgtcag gtaattatag
      481 cagtcctggt atagaatttt cccaatatgc tactctgctc aatgccttaa gacttcttat
      541 gccattccat tcactgtttt atacttatac agcatctccg catgattctg ctcctcgatc
      601 tgtatatcag ccaacagctt gcgtacacca ctgtcgccaa aggcaaatac atcatcattg
      661 tatgttcctg acaccagctt ctctgtacca atacagtctg ttgcaagaaa cgcatcgtgc
      721 tctttgtctt ccggtgaact catagctgta tagaccgctt ttggctgata atctttgcct
      781 gatctgtcat tacagttaca ttcaggcaca cttccggaaa gaatcattga caaagactca
      841 taatgcttct gttcttcctg ctggatattc cggaaaagat ttttaagctc cgggtctctg
      901 gcctgttctg cgtattttcc atacttttcc acacagcttt tctcttgtgt ctgaagatct
      961 tcgattactg aacgttcctt ttcttttaaa atcatagtct gttgtctcct tttttaagaa
     1021 atactgtatt ttgttacatg aaaatagtat ggataaaaaa tgctttttta ttctctgtat
     1081 catgacttat gacaatttca gagaatattt tgtgttactg ttcacatgcc actgtcccgc
     1141 gaggtgtttt ggcatgaata tgccaaaatc ccgagacata caagccaaaa ttccattgcc
     1201 gcaggcaatc tggaatgaat tttggctacg ttactgtgaa cggagtgaac agtaactatt
     1261 ttgctccaac accactgtat cagagtgaat ttctgtttac tttatgatat acttatatgc
     1321 acgataaaag ttttaaactt aggaagattt ataatgaaac gaattgattt tgaaaacgga
     1381 actgtgacca acaatatatt aagcgctgcg cttcccatgc tggtagcgca gatattgaat
     1441 cttttataca acattgtgga ccgtatctat atcgccagga tccatgatat aggaacaact
     1501 gcacttggcg ctgtgggctt atgctttccg atcatcatga tcattactgc tttcagtaat
     1561 ctctttggtt ccggaggtgc accaattttc tctatcaacc gcggaaaagg agattccagg
     1621 actgctgaca tgatcatgaa tactgctttc accatgttat gtggaagtgc tgctgtgctg
     1681 atgctgatcg gatttctttt tgcccgcccc ttactgactc tgtttggtgc ttcagatgat
     1741 gctctggttt atgcgtatcc ctatctgatg atttaccttc tgggtactct cccttccatg
     1801 atagcaacag gaatgaatcc ttttatcaat gcacagggat atgccattat cggtatgctg
     1861 tcagtaacag tcggtgcagt ggcaaatatt attcttgatc caatttttat ttttgttctg
     1921 gatatgggga ttaagggtgc agcgattgct actgtcatct ctcagattct ctctgccttg
     1981 cttgtatttt attttcttca tggaaaatca gaattgaagg taagatggat tcatataaat
     2041 gagatttctg agtgcaccag acatgccagg gatatcatca gtctggggtc tgctggattt
     2101 atcatgcagc tgactaacag cctggtaagt atctgctgca ataatgttct ttccgtgaca
     2161 ggcggtgata tttatatctc tgtcatgact attatatcca gtgttcgcca gatggtggag
     2221 acgcctatcc atgcgatcaa tgagggaacc tctcctgttc tcagctacaa ttacggagcc
     2281 agacgtcctg acagggtaaa gaaagcaggg gttgttctga tcattatggt tctgatctat
     2341 acaggtatca tgtggagcgt cattctcatt tcccctgaat ttctgatcgg gatcttcagt
     2401 tcagacaaac tgctattaaa ggatgcagtt cctgcactga aactttactt tgcagccttt
     2461 atttttatgg atctgcagta tattggtcag actgtattta aatcattgaa taaaaaaaag
     2521 caggcgattt ttttctccct gcttcgtaaa gtatttattg tggttccgct gacttatttc
     2581 ctgccctatg gattacacat gggcacagat ggcgtgttta tggcagagcc tgtatccaat
     2641 gtgatcggtg gttctctgtg ttttattacc atgctggtta caatattgcc tgaattaaac
     2701 aaaatgggaa actcccgata agcagaaaag tcctttacat agcttcacac atttcccaat
     2761 atgatattaa tcaggttctt atccagcatg attgtgcgat tccatgggtt aagaataatt
     2821 ccctcaatct catttacctg taatgctgaa ttgaaaagct gatctatgtc tgtcagaaaa
     2881 gtggattgta cgcttccgcc gcctttcagt tcttcctcaa agcttgtaaa tgccgcccac
     2941 cagacttttc catctccggt tttcactgtg ccgatccgga gctggttgtc tcctgtgggc
     3001 ggttctactg aaaggataaa ctggccgttt tctctcattc gtctacggat tacagtcagt
     3061 gtatgtgcca gcatttcctg agtggcttcc tgctgcagag cagcgattgc ctgctctatt
     3121 ttttcatttc cctgaagacc tttatcctga tttacattct tattatcgct catattgtta
     3181 cctttcatct gtggtgatat cacttttcag acagtgtgta agatcatatt tcagggatgt
     3241 cggcggcaaa tctctcatgc cgccggcatc ctgtcattta tttatatttc accgcagttc
     3301 catatgcaat cacttctgct gcccctgaca ttacctggga ggaaccgtat ttcacaccga
     3361 tcacagcatc cgcattcatc tctttggctt catctaccat tcgcttggtg gcaatctgtc
     3421 ttgcctcatt gagcatttcc gtataacctg tgatctctcc gcctaccaga gtcttcatcc
     3481 ctgccatgaa atcttttcct acattttttg tctgtacaac tgttcccttt acaattccca
     3541 gtgcctcaaa ctctacaccc ggaatatatt caatacttag aagcttcatc ctcttctcct
     3601 ccttcaatct ctttcatgcg tcctgcaagt gctgccagaa ttcctccgat gatcacaacc
     3661 ggtatggcga tcagaaatat cagcagtccg gtcggtatgg gatcttgtgt atttgcccac
     3721 agcataagcc ctatggtaaa tcccataagc aagatcataa ctactgctcc aatgatcgct
     3781 cctgtccttc ttttcttacg gatatctgtt ttctttatat ccataaacct tgcaccctct
     3841 ttctccatct gctcgatttc ttccagacag ttctcggcat tcaaggtatc cagagactga
     3901 tgttctgcga tcaggcgctc acacatttcc tgcattttca caaggccctc cctgcggcgt
     3961 tccagttctc ccaggtgctg ttccagacaa aactgcaggc ttttcctctt ttcaaaaaga
     4021 gctctcatct cttctattga aatatccatc ttccggaata atttgatttc ctgaagtctg
     4081 gcaatgtctg ccgtatgata ttcccggtat ccgttctctg cacgctccac attcaggaga
     4141 ccctgctctt cataaaaacg gatattcttt ctggtgatgc ctaccagctc ttccacctgt
     4201 ttgatcttca tactgctacc tcttgctttc ctgacagcag atacagctga tctgctgctc
     4261 tact
//
LOCUS       DBMDXG010000158         4247 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4952_length_4247_cov_1.493005, whole genome shotgun sequence.
ACCESSION   DBMDXG010000158 DBMDXG010000000
VERSION     DBMDXG010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4952_length_4247_cov_1.493005"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="ACZPD3_05475"
     CDS             <1..713
                     /locus_tag="ACZPD3_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1415486.1"
                     /translation="ILSRNLSRENLALKEKMAADGAFEERFAVVNLILGALREKAEEY
                     GQADHQLEVLYEMLLHLRTQVRKNAEEEKLGISLLEEFVQEQENSLQIKVKMELISVR
                     EQKYQETAIRRLREYILTAKKEHVRSAENGFKRISKCFEKEAVCREDMIQKLQGELQN
                     AFLFIKESFGENQEMLLFVTGITADKSMTSFIAGNGCPAYFEHSEMLLYNKEENELRK
                     ACLEAVHERLQEESRDRV"
     gene            869..>1132
                     /locus_tag="ACZPD3_05480"
     CDS             869..>1132
                     /locus_tag="ACZPD3_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1415487.1"
                     /translation="MLTVVLIAAICIFCYAGYNLFHIYTEYKKGTDEYNSITQMAVTE
                     RDPDGEAAGPEAGSELKAPMDIDFASLKSVNDDVVGWIYVEAVP"
     assembly_gap    1133..1142
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1143..1630
                     /locus_tag="ACZPD3_05485"
     CDS             <1143..1630
                     /locus_tag="ACZPD3_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR1415488.1"
                     /translation="VHGKDNETYLHRTYEKNYNFAGTIFVDYENKGDFSDCNTIVYGH
                     NMKNGSMFAQLKKFTQDEETYKKSKYFWIFTPEKNYRYEIISAYTTGVNSDTYTLFKG
                     PGEEFEKYLEKIRGYSEIRTDAEGMNIKDKIITLSTCTGNEATRYVVQGKRVDTLDVG
                     Q"
     gene            1752..2474
                     /locus_tag="ACZPD3_05490"
     CDS             1752..2474
                     /locus_tag="ACZPD3_05490"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR1415489.1"
                     /translation="MAGEVERLQELVDKYDNIVFFGGAGVSTESGIPDFRSQDGLYHQ
                     KYDYPPETILSHTFFMRKPEEFFKFYRDKMLCDTAKPNAAHLKLAELEQAGKLKAVIT
                     QNIDNLHQMAGSKKVLELHGSVYRNYCMKCHRFYDFAHMKASTGVPRCECGGIIKPDV
                     VLYEEGLDNQTINEAVKAISEAQVLIIGGTSLAVYPAAGLIDYFRGEHLVVINKSPTP
                     RDRYADLLIQGPIGQVFSQIHC"
     gene            2506..2697
                     /locus_tag="ACZPD3_05495"
     CDS             2506..2697
                     /locus_tag="ACZPD3_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR1415490.1"
                     /translation="MGLPYEVGDIVTLKKVHPCGSRDWEILRVGADFRLKCTGCGHQI
                     MVPRKMVEKNTKNLIKKEK"
     gene            2808..3095
                     /gene="rpsF"
                     /locus_tag="ACZPD3_05500"
     CDS             2808..3095
                     /gene="rpsF"
                     /locus_tag="ACZPD3_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR1415491.1"
                     /translation="MNKYELALVVSAKVEDEVRDAVVEKAKGYITRYNGTITEVEEWG
                     KKKLAYEIQKMHEGFYYFIQFEADAQCPAEVERHVRIMDNVLRYLVVRKDA"
     gene            3175..3636
                     /locus_tag="ACZPD3_05505"
     CDS             3175..3636
                     /locus_tag="ACZPD3_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1415492.1"
                     /translation="MNKVILMGRLTRDPEVRYSAGENALAIARYTLAVDRRFRRDGEA
                     TADFISCVSFGRTAEFAEKYFRQGLKIIVSGRIQTGSYTNRDGQKVYTTEVVVEEQEF
                     AESKAASDNYAASHPQTSSPAPAPSMPAPGAASADGFMNIPDGIDEELPFI"
     gene            3673..3936
                     /gene="rpsR"
                     /locus_tag="ACZPD3_05510"
     CDS             3673..3936
                     /gene="rpsR"
                     /locus_tag="ACZPD3_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR1415493.1"
                     /translation="MAYNRGERPDSPMKRRGGRRRKKVCVFCGKENNEIDYKDVAKLR
                     KYVSERGKILPRRITGNCAKHQRALTVAIKRARHMSMMPYIQD"
BASE COUNT         1449 a          720 c         1053 g         1015 t
ORIGIN      
        1 cgatcctgtc cagaaatctg agccgggaga atctggcgct gaaggagaag atggctgcag
       61 atggagcttt tgaggagcgt tttgcagttg taaatctgat cctgggggca ttgagagaaa
      121 aagcggaaga atacggacag gcagatcatc agcttgaggt actctatgag atgcttctgc
      181 atctgagaac tcaggtgaga aagaatgctg aggaagagaa gctgggaatt tcattgcttg
      241 aagaatttgt gcaggaacag gaaaacagtc tgcagatcaa agtaaagatg gaactgatct
      301 cagtaagaga acagaaatat caggagacgg caatccgcag gctgagggaa tatattctga
      361 cagcaaagaa agaacatgtg cggtcagctg aaaatggatt taagcggatc agtaaatgtt
      421 ttgaaaaaga agcagtatgc agagaagata tgatccagaa actccaggga gaactgcaga
      481 atgccttttt atttattaaa gagagctttg gcgaaaatca ggaaatgctg cttttcgtga
      541 cagggatcac agcggataag tcaatgactt cgtttattgc cggaaatggc tgtccggcat
      601 attttgaaca cagcgagatg cttttatata ataaagaaga aaatgaactg cgaaaagcgt
      661 gtctggaggc agtgcatgaa agactacagg aagaaagccg tgaccgggta taatatctgt
      721 tacagacagt atctgaaaac ggcagtcagt atattattga tagaagcaaa tcagacagca
      781 gctgatacat agcagcagaa atacgctgcg gaaatggagg aagaatgtca gaaaaacagc
      841 ctgaaaaaag aagacagcag gggatgttgt gttgacggtt gttctgatcg cagcaatctg
      901 tatattctgt tatgcaggat ataatctgtt tcatatttat acagaatata agaaaggtac
      961 ggatgagtac aattcaatca cacagatggc agtaacagaa cgagatccgg atggagaagc
     1021 agcagggccg gaggcaggca gtgaactgaa agcgcctatg gatattgact ttgcatctct
     1081 taaaagtgta aatgatgatg tagtgggatg gatttatgtg gaagcagtgc cgnnnnnnnn
     1141 nnttgtgcat ggcaaagaca atgaaactta tcttcacaga acctatgaga agaattacaa
     1201 ttttgcaggt actatctttg tggattatga aaacaaaggg gatttcagtg actgtaatac
     1261 gatcgtgtat ggccataata tgaaaaatgg ttccatgttt gcacagctta agaaatttac
     1321 acaggatgag gagacttata agaagagcaa atatttctgg atctttacac ctgagaaaaa
     1381 ctacaggtat gagattatct ccgcgtacac cacaggtgtg aacagtgata cctatacact
     1441 gtttaaggga cctggtgagg aatttgaaaa atatctggag aagatcagag gatattccga
     1501 gatccggaca gatgcagaag gaatgaacat aaaggacaag atcattactc tgtctacctg
     1561 tacaggaaac gaggcaaccc gttatgttgt ccagggaaaa cgtgtggata cacttgatgt
     1621 cgggcagtag aattatgaag agcagttacc tgaatcagaa gaaaatcgta ttgctgtaaa
     1681 agcagacgat aaaaattcag atgataaaat ttcagtaaca gtgtttggaa aaaataaagg
     1741 aggaattcta aatggcagga gaagttgaga gattacagga acttgtagac aaatatgaca
     1801 atattgtctt cttcggcgga gcaggtgttt ccacagagag cggaatcccg gatttcagaa
     1861 gtcaggacgg actttatcat cagaaatatg actatcctcc ggaaacgatc ttaagccata
     1921 cattctttat gagaaagccg gaagaatttt tcaaattcta cagagacaag atgctttgtg
     1981 acactgcaaa accaaatgca gcacatctga agctggcaga actggagcag gcaggaaaac
     2041 tgaaagcagt tattacccag aatatcgaca atctgcatca gatggcgggt agtaagaaag
     2101 tgctggagct tcatggaagt gtataccgca attactgcat gaagtgtcac agattttatg
     2161 attttgccca catgaaagca tccacaggtg ttccgagatg cgaatgtggt ggaattatca
     2221 agccggatgt ggtattgtat gaagaaggtc tggacaatca gacgatcaat gaagcagtga
     2281 aggcaatctc agaggcacag gtgctgatca tagggggaac ttccctggct gtttacccgg
     2341 cagcaggact gatcgattac ttccggggag agcaccttgt agtgatcaac aaatccccga
     2401 ctccgagaga cagatatgca gaccttctga tccaggggcc gatcggacag gtattttcac
     2461 agattcactg ttagtgtcaa agttatataa gaggagtaag agattatggg acttccctat
     2521 gaagttggtg atattgttac attaaagaaa gtccatccct gcggaagcag agactgggaa
     2581 atcttaaggg taggtgctga tttccgcctg aaatgtacag gatgcggaca tcagatcatg
     2641 gtaccgcgca agatggttga gaaaaatact aaaaatttga taaaaaaaga aaaatagtac
     2701 tttacagaca cgaaaaacta tgttagaatc aacactcgtg atatatttta tatccttgct
     2761 ctttgacagg caaagggcca acagaccata aggaggtaaa gcaagacatg aacaagtacg
     2821 agttagcatt agttgttagt gcaaaagttg aagacgaagt acgtgatgca gttgttgaga
     2881 aagcaaaagg atacatcaca cgttacaacg gcactatcac agaagtagaa gaatggggta
     2941 agaaaaaatt agcttacgaa attcagaaaa tgcatgaagg cttctactat ttcattcagt
     3001 ttgaagcaga cgcacagtgc ccggcggaag ttgaaagaca cgttcgcatc atggacaatg
     3061 tattaagata cttagtcgta agaaaagacg cataatctta tacagggtgg attttagaag
     3121 gcaccattaa ttgatccgtt caaccattca aagcagaaac gaagaggtat ttatatgaac
     3181 aaagtaattt taatgggacg cttaacaaga gatcctgagg tgagatattc cgcaggagag
     3241 aatgcattgg caattgccag atatacactg gcagtagaca gaagattccg ccgtgacggt
     3301 gaagcaacag cagactttat cagttgtgta tcctttggac gtacagccga gtttgctgag
     3361 aagtatttcc gtcagggact taagatcatt gtttcaggac gcattcagac aggcagctac
     3421 accaatcgtg atggccagaa ggtttatacc acagaggtag tagttgagga acaggagttt
     3481 gctgagagca aggcggccag cgataattac gcggcttccc atcctcagac atcatcccct
     3541 gcacctgcac catccatgcc ggctccggga gcagccagtg cagacggttt catgaacatt
     3601 ccggatggaa ttgatgaaga actgccgttt atttaataca aactgataga ataaaatagg
     3661 aggaaagcaa tcatggctta caatagaggc gaaagaccag actctccaat gaagagaaga
     3721 ggcggccgca gaagaaaaaa agtttgtgta ttctgtggta aagagaataa cgagatcgac
     3781 tacaaggatg tagcaaaatt aagaaaatat gtttctgaaa gaggaaaaat ccttccaaga
     3841 agaattacag gaaactgtgc aaaacatcag agagcactga cagttgctat caagagagca
     3901 cgtcatatgt ctatgatgcc atacatccag gactaattct gaagatgata aatgctgctc
     3961 cgcatttggc ggagcagcat ttttgcatta tttacgagaa ttgctataga acagaatgaa
     4021 cagtaacaca ataaccatta tttttgtgcg ttcttctgtt gctatggtaa attctgttca
     4081 aatctgttat aatatgattc aaagggcgtt tttcacagaa cctgcctata tgcagcacat
     4141 ttgctgtgta caggcgaaac aaatagaatg atgaagaacg gaaagaaaga tatgagtgat
     4201 aaactgaaat taaaagggca tatgaaagca ttcatgcggt ggcctct
//
LOCUS       DBMDXG010000159         4232 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_4972_length_4232_cov_1.926894, whole genome shotgun sequence.
ACCESSION   DBMDXG010000159 DBMDXG010000000
VERSION     DBMDXG010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4972_length_4232_cov_1.926894"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(195..1205)
                     /gene="pta"
                     /locus_tag="ACZPD3_05515"
     CDS             complement(195..1205)
                     /gene="pta"
                     /locus_tag="ACZPD3_05515"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR1415494.1"
                     /translation="MGFIDTLKERAKANVKTIVLPETEDKRTLAATEKILKEGIAKVI
                     LVGNEEAVKKSAAEDGYDISGAQIVDPATSEKTQGYIDKLVELRQKKGMTPDQAKEIL
                     LNQYLYYGVMMVKMGDADGMVSGACHSTADTLRPCLQILKTKPGTKLVSAFFLMVVPN
                     CEYGANGAFVFADSGLNQNPTSEELAAIAASSAESFELLVQEKPIVAMLSHSTKGSAK
                     HADVDKVVEATKIAKEQNPELALDGEFQLDAAIVPSVGASKAPGSEVAGKANVLVFPD
                     LDAGNIGYKLVQRLAKAEAYGPVTQGIAKPVNDLSRGCSADDIVGVVAITAVQCQADD
                     NK"
     gene            1416..2783
                     /locus_tag="ACZPD3_05520"
     CDS             1416..2783
                     /locus_tag="ACZPD3_05520"
                     /EC_number="6.3.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetate ligase"
                     /protein_id="HIR1415495.1"
                     /translation="MKTAGIIAEYNPFHKGHEYQIRYTKEKLKADYVIVAMSGDYVQR
                     GTPALISKHTRAEMALRCGADLVLEMPVSVSTASAEAFAMGGVSLLDGLGVVDMLCFG
                     SESGEISALKELAEILVEEPEEYKKLLKSFLSEGLTFPAARSQALTEYFKNPRNFSGD
                     DFDGVLTPLLNEVTQILNTPNNILGIEYCKALLRLNSQIRPVTIRREGMGYHETTVPE
                     GDSASSSPDLQSSTDFFASATAIRSLIQNPGDGHSEASSDINNPVRNPDTKTANILSS
                     QIPPDAFYVFKKALDSGEFLTENSLDSILSYCLMKENVESLSSYMDVSEDLARRIINQ
                     QNLLLSFSQSVSVLKTRELTQTRIQRALLHIILNIHTAPTQIPFARVLGFRRESSELL
                     SQIKQHSQIPLITKLADAQNLLDSEGNQILSETVFSSNLYEKLLCLKTGRKFCHESQK
                     QLIIL"
     gene            2864..3652
                     /locus_tag="ACZPD3_05525"
     CDS             2864..3652
                     /locus_tag="ACZPD3_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415496.1"
                     /translation="MKANSGLKKNRTKIQMVLTALVILSVVRQFFLGNYHNMFLGILT
                     LILFMVPQFLDRKLNVTIPPGLETVILIFIFSAEILGEINAFYVKIPIWDTILHTTNG
                     FLMAAIGFALIDLFNRSDRFSIKMSPYFVAFFAFCFSMTVGVMWEFFEFSMDWFFGMD
                     MQKDWIVPAINSVKLNPTGANVPIHVDVQSLAVNGETWNLGGYLDIGIVDTMKDLIVN
                     FIGAVVFSIIGILYLRNRGKGKLAASLIPQVRSKQEEELSSKNQ"
     gene            complement(3662..4075)
                     /locus_tag="ACZPD3_05530"
     CDS             complement(3662..4075)
                     /locus_tag="ACZPD3_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR1415497.1"
                     /translation="MKRIKVLGSVLKRTRADKIIIGFIVFIFAIAAVIQLVEPDINRY
                     GDALWYCYAVISTAGFGDVVAVTFIGKMCSVLLTIYSLFVVAIATGVVVNFYTQMVEL
                     QRKETLAMFMDQLERLPELSREELENISRKVRQFR"
BASE COUNT         1203 a          916 c          840 g         1273 t
ORIGIN      
        1 cataatcctg acacaagtct tcgaatattt ctgtcagctt ttcctttcat attataatat
       61 tattctcatc atttccaggg aaatatgagt aatgatatca aaggcatcag aatcagaaat
      121 atctcctccg atgcctttaa cattatgtag tcaatgaaat aattctcagt ccgttttacg
      181 gtctgactga aatcttattt attgtcgtca gcctggcact gtactgctgt gatagctacc
      241 acaccaacga tatcgtctgc agagcatcca cgggacaggt catttacagg tttagcgata
      301 ccctgggtta caggtccgta agcttctgct tttgcaagac gctgtacaag tttgtatccg
      361 atgttacctg catcaaggtc tgggaataca agtacgttag ctttacctgc aacttcgctt
      421 ccaggagctt tagaagcgcc tacgcttgga acgatagctg catcaagctg gaattctccg
      481 tcaagtgcaa gttccggatt ctgctctttt gcgatctttg tagcttctac tactttatct
      541 acgtcagcat gttttgcact gcctttagtg gagtgtgaaa gcattgcaac gattggtttc
      601 tcctgaacaa gaagttcgaa ggattctgca gaagatgctg cgattgctgc aagctcttct
      661 gaagtcgggt tctggttcag accggaatca gcaaatacaa atgcgccgtt tgcgccatat
      721 tcgcagttcg gaactaccat taagaagaat gcagatacaa gttttgtacc tggttttgtt
      781 ttcaggatct gaagacatgg tcttaatgta tcagctgtag agtgacatgc accggatacc
      841 ataccgtctg catcgcccat ttttaccatc attacaccat agtacagata ctggttaaga
      901 agaatctctt tagcctgatc cggtgtcatg ccttttttct gtcttaattc tactaacttg
      961 tcaatgtatc cctgtgtttt ctctgatgta gcaggatcta cgatctgtgc gccggaaatg
     1021 tcatatccgt cttcagctgc actcttttta acagcttctt cgttaccaac aaggataacc
     1081 tttgcaattc cttcttttaa aattttctct gtagctgcca gagttctctt atcttctgtc
     1141 tccggaagta cgattgtttt tacgtttgct tttgctctct ctttgagtgt gtcaataaat
     1201 cccatgatta aaatggctcc tttcatatag cagttctgat atccaatgtc aattttctat
     1261 caattatctg cgctgttttc attataactc ctgaagcctt gtatttcaag catctgaatg
     1321 gtagaatatg acataaaaaa gtatttttta agatgcaata tggttaaaaa aagagaactc
     1381 tacagaattt caggaaggag catctgtgta aatttatgaa aacagcagga attattgcag
     1441 aatataatcc ttttcacaaa ggacatgaat atcagatccg gtatacaaaa gaaaagctaa
     1501 aagcagacta tgttatcgtt gccatgagcg gagattatgt gcagcgtggc acccctgctc
     1561 tcatctccaa acatacacgt gcagaaatgg ctctgcggtg cggcgctgac cttgtgcttg
     1621 aaatgccggt atctgtctct actgcaagtg cggaagcctt cgcaatggga ggtgtttccc
     1681 tgttggacgg tcttggcgtt gttgatatgc tctgctttgg aagtgaatcc ggggagatca
     1741 gcgcactgaa agaactggct gagatccttg tagaagaacc tgaagaatat aaaaagctac
     1801 tgaaaagctt tttaagcgaa gggctgactt ttccggctgc cagaagccag gcattgacag
     1861 aatattttaa aaatccgcgc aacttcagtg gtgacgattt tgacggggta cttactcctc
     1921 tgttaaatga agttacacag atcctgaata caccgaataa tattctgggg atcgaatact
     1981 gcaaggcact tctccgcctg aacagccaga tccgccctgt taccatccgc agggaaggaa
     2041 tgggttatca tgaaaccact gtccctgaag gagattcagc aagctcctcc cctgatttac
     2101 agtcatctac tgatttcttt gcatctgcaa ctgctatacg cagtcttatt cagaatccag
     2161 gagatggcca ttcagaggca agctcagaca taaataatcc agtcaggaat ccggatacca
     2221 aaactgccaa tatattatca tcccagattc caccagatgc tttttacgtt ttcaaaaagg
     2281 cgctggactc cggtgaattt ctcacagaaa attctctgga ctctattctt tcttactgcc
     2341 ttatgaaaga aaatgtagaa agcctttctt cctatatgga tgtttccgag gatctcgccc
     2401 ggcgaatcat aaatcagcag aatcttctgt tgtccttttc ccaatctgta tctgttttaa
     2461 aaaccagaga attgactcag acaaggatac agagggcatt gcttcatatt atattaaata
     2521 tccacactgc acccactcag attccctttg ccagggttct tggtttccgc agggaaagca
     2581 gtgaactgct aagccagatc aagcagcaca gccaaattcc tcttatcacc aagcttgcgg
     2641 acgcccaaaa tcttttggac agtgaaggta atcagatttt atctgaaact gttttttctt
     2701 ccaatttata tgaaaaactt ctgtgtctga aaaccggcag aaaattctgc cacgagtccc
     2761 agaaacagct tattatccta taattcttgt tgttttgatg cacttatgtt aaaatacaaa
     2821 cagtgaggat aaggatataa aataaggaag gaggaaaaga aaaatgaaag ctaattcagg
     2881 tttgaaaaag aacagaacaa agatccagat ggtacttacc gccctagtaa tcttatccgt
     2941 tgtacgtcag tttttcctgg gaaattacca caatatgttt ctgggaatct tgacattgat
     3001 cctttttatg gttccccagt ttctggacag gaaactgaac gtcacaatac caccgggact
     3061 ggaaacagtg attctgatct ttatcttttc agcagaaatt cttggcgaaa tcaatgcctt
     3121 ttatgtaaaa atccctatct gggataccat tttacatact actaacggtt ttctgatggc
     3181 tgccatcgga tttgcactga ttgatctttt taaccgcagc gacaggtttt ccattaaaat
     3241 gtcaccgtat tttgtagctt tttttgcatt ctgtttttca atgactgttg gtgttatgtg
     3301 ggaattcttt gaattcagca tggactggtt ttttggaatg gatatgcaga aggactggat
     3361 cgtaccggcc atcaactctg tgaagctgaa tcctaccggt gcaaatgttc cgatccatgt
     3421 agatgttcag tccctggccg ttaacggcga gacctggaat ctgggaggct atcttgatat
     3481 tggtattgtt gataccatga aagatctgat cgtaaacttt atcggcgcag tcgtattttc
     3541 tatcattggt attctgtatc tgcgaaaccg cggaaaagga aagctggcag cttctctgat
     3601 cccgcaggta cgcagcaagc aggaagaaga actttcttcc aaaaatcagt aagatccaca
     3661 gctaccgaaa ctggcgcact tttctggaaa tattctccag ctcttctctg gaaagctcag
     3721 gaagtctttc cagctgatcc ataaacatgg caagtgtctc ttttcgctgc aattccacca
     3781 tctgggtgta gaaattaacc actaccccgg tggcaatggc aactacaaaa agcgaataga
     3841 tcgtcagaag aacggaacac atttttccaa taaaagtaac cgcaaccaca tcgccaaatc
     3901 cggcagtgga gatcaccgca tagcaatacc acagtgcatc tccgtatcgg ttaatgtccg
     3961 gttcaaccag ctggatcact gctgcgatag caaaaataaa cacaataaaa ccaatgataa
     4021 ttttatctgc acgagtccgt tttaacacac tgcctaatac tttaattctt ttcatgcact
     4081 tgtcctccaa taaaataata tactgcctga aattaattga agtatagtat aaaacggttt
     4141 cacttgcaat atgaaaggag cttaacagta tgaaagataa taaagcacta aaatacctca
     4201 ctttgatcac cggagtttta acattgttat ta
//
LOCUS       DBMDXG010000160         4161 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5071_length_4161_cov_1.775123, whole genome shotgun sequence.
ACCESSION   DBMDXG010000160 DBMDXG010000000
VERSION     DBMDXG010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5071_length_4161_cov_1.775123"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="ACZPD3_05535"
     CDS             complement(<1..1178)
                     /locus_tag="ACZPD3_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR1415498.1"
                     /translation="MKKSLRNYRLFFHRMGLLFLFIPLIVLILSIVLPDRGFSEKENR
                     VLSSLPHLNGSQIASGGFEKQFETYENDQFPLRDLWITLKAGTDRLMGKVESNGVYVG
                     KSGYLMEEFKAPDQTQYDATVKAMTDFAQKHSDLKQYALIAPNSVNILSDRLPAFAPV
                     QDQNSWLDSLSASLTDAGVTFIDVRSTFKDHKMEDLYYHTDHHWTTQGAYYAYLKAAK
                     DMGIDTSADTYDKAPVTRSFQGTLSAKSGFRSGEKDEIDVFLPTGDQALSSVVNYVDE
                     QKKSASFYDTEKLETRDKYALFFGGNHAQIKISTPTETDNTLLVLKDSYANSFIPFLA
                     QHYRKIIMIDPRYYFGDLEQLMQVENVQEILYLYNANTFFTDTSLELALTTAEQDSSD
                     T"
     gene            complement(1191..2579)
                     /locus_tag="ACZPD3_05540"
     CDS             complement(1191..2579)
                     /locus_tag="ACZPD3_05540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR1415499.1"
                     /translation="MVFNSIFFIFCFLPVFMLIYYLVPGKLRNLLLFLGSLIFYAWGE
                     PVYVILMLFSSIFNYYMGTELERLYYDDRKQKINLIFSIIINLAVLVFFKYYGFLLNT
                     IGGIIGIHIPHPELSLPIGLSFYTFRNLSYLFDIYLSKVSAQRNFLTFAVYSTMFPYT
                     SAGPIVRYTDIETQLKQRTINISKFGTGAELFVKGLAKKVLLADNLSVLYSSICGHPQ
                     MSVFTSWLGILAYTMQLYFDFSGYSDMAIGLGKMLGFDFNKNFDYPYISTSVSEFWRR
                     WHISLGSWFRDYIYIPLGGNRVSTLKHIRNILVVWALTGLWHGASWNFVLWGVYYGLF
                     LLLEKFVLQKYLKKIPSWLCTVYTMLVVMIGWVFFSQTDFGALGHYLGTMFGIGASGF
                     IDKTALYYLKTGFILLLISILMCRPAPYQYFKRLVRRRPVAAVIINLILFALSIAYMV
                     YNSYTPFLYAKF"
     gene            complement(2735..3211)
                     /locus_tag="ACZPD3_05545"
     CDS             complement(2735..3211)
                     /locus_tag="ACZPD3_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR1415500.1"
                     /translation="MKNYLHIFIKGAFVLFLIAYLTVLYTSDSAKNVPMEQIAQTMEQ
                     DSDITSLNKEARSDLKHYYQTDDRNIDGYFFYKAASPMAVEEICIMKATDNTQANTLL
                     ENANAHLSGQKQVFEGYGTDQMALLNNAVVGKKGNYVYYMCGADAQNWRTAFLSMI"
     gene            complement(3208..4002)
                     /locus_tag="ACZPD3_05550"
     CDS             complement(3208..4002)
                     /locus_tag="ACZPD3_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1415501.1"
                     /translation="MKKQPKNKVLFLIPVGVILAGAVIFLCTRGNENDSVCKENVARL
                     QALENSDISQTEEQLQALKQTTGISSGSDASDGSGSTLLSDVEIRQVFAGNVIIGDSI
                     TESIVEYGYLDTDVVVSKRGLNVGAADDQISTAIALNPSHVFMAFGSNDLEIYGSASS
                     EFIDAYRTQIKKIQTALPDVPIYINCILPITDEAIAQTPDLGYYPDYNEGLIKLCQEM
                     GCTFIDNSTIVTDSSENLYEPDGEHVIQDYYPKWLTNMAQIAGLKA"
BASE COUNT         1292 a          817 c          866 g         1186 t
ORIGIN      
        1 gtatcagaag aatcctgttc agcagttgtc agggcaagct ccagagaagt gtctgtgaag
       61 aaggtattag cattatacag gtacagaatt tcctgaacat tttcaacctg cattaactgc
      121 tccagatctc caaagtaata acggggatca atcattatga tctttcggta atgctgagca
      181 agaaatggga tgaagctgtt tgcgtatgaa tccttaagca caagcagtgt attatctgtt
      241 tcagtaggag tactgatctt tatctgagcg tggtttccac caaagaataa tgcatattta
      301 tctcttgtct caagtttctc ggtatcatag aagctggcac tttttttctg ttcgtctaca
      361 taattaacta cagaagagag cgcctgatct ccggtgggga gaaatacatc gatctcatct
      421 ttttcgccgg agcggaaacc gcttttggca gacagtgttc cctgaaaaga acgggtgaca
      481 ggtgctttgt cataagtgtc tgctgaagta tcaattccca tatctttggc tgccttaaga
      541 taggcataat aagcaccttg ggtagtccag tgatgatcag tgtgataata gaggtcttcc
      601 attttgtgat ctttaaaagt gctgcgaaca tcaataaagg tcacacctgc atcggtcagg
      661 gaggcagaga ggctgtcaag ccaggaattc tgatcctgga caggggcaaa agcaggaagc
      721 ctgtcgctca ggatattcac agaatttggg gcgatcagtg catattgttt cagatcagaa
      781 tgtttttgcg caaagtcagt cattgctttt acagtggcgt catactgtgt ctgatcaggc
      841 gctttaaatt cttccataag atatccggat tttccaacgt atacgccatt agattctact
      901 tttcccatca gacggtcagt tccggctttc agggtaatcc acagatcgcg aagtggaaac
      961 tgatcatttt cataggtttc aaattgtttt tcaaatccgc cggaagcaat ctgactgccg
     1021 tttaaatgag gcagggaaga aagtacgcgg ttttcttttt cagaaaatcc tctgtcagga
     1081 agcacaatgc tcagaatgag tacgatcaag gggataaata aaaacaaaag tcccattctg
     1141 tggaaaaata aacgataatt tcttaatgat tttttcatat tgtttacctt ctaaaatttt
     1201 gcatataaaa atggtgtgta agagttataa accatatagg caatcgataa tgcaaataaa
     1261 ataagattta tgatcactgc agcaacaggt ctgcgtctga ccagacgctt aaagtactgg
     1321 taaggagcag gacggcacat aagaatactg atcagaagaa ggataaatcc tgtcttgagg
     1381 tagtaaagtg cagttttgtc tatgaaaccg gaagctccga tcccaaacat agttccaagg
     1441 taatggccaa gggcaccaaa gtctgtctgg ctgaagaata cccatccgat catgactaca
     1501 agcatagtgt atacagtgca aagccaggat ggtatttttt tcagatattt ctgaagtaca
     1561 aatttttcga gtaaaaggaa gagtccgtaa taaacacccc atagtacgaa attccagctt
     1621 gcaccatgcc acagaccggt aagtgcccat accacaagga tatttcggat atgtttcaga
     1681 gtggagaccc gattaccgcc aagcgggata taaatataat ctctgaacca gcttccaaga
     1741 gaaatatgcc agcgtctcca gaattcagaa acactggtgg aaatataggg ataatcaaag
     1801 ttcttattaa aatcaaatcc cagcattttt cccagaccga tggccatatc cgaatagccg
     1861 ctgaagtcaa aataaagctg cattgtataa gccagaatgc caagccatga agtaaataca
     1921 gacatctgtg gatgaccaca gatactgctg tataaaaccg agagattatc tgccagaaga
     1981 actttctttg caagaccttt gacaaaaagc tctgcaccgg taccgaattt ggagatattg
     2041 atggtgcgct gcttcagctg cgtctctata tccgtatatc gtacgattgg tccggcagag
     2101 gtataaggaa acattgtgct gtatacggca aaggtcagga aatttcgctg cgcactgacc
     2161 ttgcttaaat aaatatcaaa aagataggac aaattacgga aagtgtagaa agaaagtcct
     2221 atcggcagcg aaagctccgg atgagggata tggataccga tgattcctcc gattgtgtta
     2281 agtaagaaac cataatattt aaaaaacaca agtactgcaa gatttataat gatcgagaaa
     2341 atcagattaa ttttctgttt cctgtcgtca taatataaac gttccagttc tgtacccata
     2401 taatagttga agatactgga aaacagcatg aggatcacat atacaggttc tccccaggca
     2461 taaaagatca gactgcccaa aaacagcagc agattgcgga gctttcccgg taccaggtag
     2521 tagatcagca tgaaaaccgg aagaaagcag aatataaaaa atatactgtt gaataccata
     2581 ttaaaagttc tccaatctgg atattatgaa tattatagta attctcttaa ctgcattttc
     2641 tcttaacggt attattaaga ggactgccat agtaatggtt ctgcagtcca ttagcgtaaa
     2701 atgcggaaca gttacgagga ttaaagtaaa atgcttatat catggaaaga aaggcagtgc
     2761 gccagttctg tgcgtctgcg ccgcacatat aatatacata gtttcctttt tttccaacaa
     2821 ctgcattgtt cagaagagcc atctgatctg tgccatagcc ctcaaatacc tgcttctgac
     2881 cggagagatg ggcgtttgca ttttcaagca aagtatttgc ctgggtattg tcagtggcct
     2941 tcataataca gatttcttct actgccatgg gtgaagcggc tttgtagaag aaataaccgt
     3001 caatattgcg gtcatctgtc tgataataat gtttcagatc agaacgtgct tctttattta
     3061 aagaagtgat atcagaatcc tgttccatag tctgtgcaat ctgttccatg ggtacattct
     3121 ttgcgctgtc ggaggtatac aggacagtca gatatgcgat aaggaaaaga acaaatgcac
     3181 ctttgataaa aatatgtaaa taatttttca tgcctttaac cccgcaatct gtgccatatt
     3241 tgtcagccat ttgggatagt agtcctgaat cacatgttca ccgtccggtt catacagatt
     3301 ctctgaactg tctgttacaa tcgttgaatt gtcaataaag gtacatccca tttcctgaca
     3361 gagtttgata agtccttcat tataatcagg gtaatatcca agatcaggag tctgtgcgat
     3421 cgcttcatca gtaatcggca gaatgcagtt gatataaatt gggacatccg gtaaggctgt
     3481 ctggattttc tttatctgcg tcctgtatgc atcaataaat tctgatgaag ccgaaccata
     3541 tatttccaag tcgttggaac caaatgccat gaatacatga gagggattca gagcaattgc
     3601 cgtactgatc tgatcatcgg ctgcacctac atttaatcct cgtttgctga ctaccacatc
     3661 cgtatccaga tatccgtatt ctacgatgga ttctgtgata gagtcaccga tgatgacgtt
     3721 tccggcaaaa acctgtcgga tttctacatc acttaacagg gtagatccgg aaccgtctga
     3781 agcatcagaa ccggaagaaa tgccggttgt ctgtttcaga gcctgaagct gttcttctgt
     3841 ctgtgaaatg tctgaatttt ccagagcctg aaggcgggct acattttctt tacagacaga
     3901 atcattctca tttccgcggg tgcataaaaa aatcactgca ccggcgagta taacaccgac
     3961 agggataaga aataaaactt tattctttgg ttgttttttc aaaataaatc cttcctatgg
     4021 aaaaatatcc attttatgac atagttttat ttatagcaat actctcaaat gtattattta
     4081 tgagtatcgc tataattata atacgcacta aaaaaacttc aaggaaaaat tcacaaattt
     4141 tacacaaagt catgcagttg c
//
LOCUS       DBMDXG010000161         4152 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5080_length_4152_cov_1.749198, whole genome shotgun sequence.
ACCESSION   DBMDXG010000161 DBMDXG010000000
VERSION     DBMDXG010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5080_length_4152_cov_1.749198"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /gene="argC"
                     /locus_tag="ACZPD3_05555"
     CDS             complement(<1..217)
                     /gene="argC"
                     /locus_tag="ACZPD3_05555"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266565.1"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR1415502.1"
                     /translation="MIKAGIIGATGYAGNEIVRLLLGHKDVEVAWYGSRSYIDQKYAD
                     VYQNFFKLVDAKCMDDNMEALADEVDVI"
     gene            678..1904
                     /locus_tag="ACZPD3_05560"
     CDS             678..1904
                     /locus_tag="ACZPD3_05560"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR1415503.1"
                     /translation="MKEKVVLAYSGGLDTTALIPWLKETFDYDVVCCCVNCGQGNELD
                     GLDERAKLSGASKLYIEDIVDEFCDDFIVPCVQAGAVYEHKYLLGTSMARPAIAKKLV
                     EIARKEGAVAICHGATGKGNDQIRFELGIKALAPDIKIIAPWRMTDKWTMQSREDEIA
                     FCKAHGIDLPFDASHSYSRDRNLWHISHEGLELEDPSQAPNYDNMLVLGVTPEKAPDK
                     DTEITMTFEQGVPKTLNGKEMKVSEIITELNKLGGENGIGTVDIVENRVVGMKSRGVY
                     ETPGGTILMAAHEQLEELTLDRETMETKKKLGSQFAQVVYEGKWYTPLREAIQAFVES
                     TQKYVTGEVKMKLYKGNIIKAGTTSPYSLYNESLASFTTGDLYDHHDADGFITLFGLP
                     LKVRAMMLKEVEQNKK"
     gene            complement(2055..2429)
                     /locus_tag="ACZPD3_05565"
     CDS             complement(2055..2429)
                     /locus_tag="ACZPD3_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1415504.1"
                     /translation="MDILKGKRYDIMNVLWEEGRPLSAFEINDIAPELKMPTVRRCLE
                     LLLKENLIQVAGTSMNGKVYARNYTPLLSREDYLKGNAKSRKINPVEMMNALLESDGI
                     TVEELEKLQELIDKKRAELESR"
     gene            complement(2593..3741)
                     /locus_tag="ACZPD3_05570"
     CDS             complement(2593..3741)
                     /locus_tag="ACZPD3_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR1415505.1"
                     /translation="MQLDKENIKKIRWLIAFAILLYLGVQNLHIVISTVRVLLGFLFP
                     FIIGFGIAFILNIPMKFIEHHLFGKALKQEKKTAQKLARPVSLVLSICFVICIIVIVM
                     LVVVPELGATFVNIAKKIEENIPVFQKWIDNVFGNNPEVVKWAQSLDIEPGKIIDSVL
                     GVLKNGVNNIVSSTVSITMGLLTTAMNVSIGFVFACYVLLQKEKLMQQVKKAMYAMFP
                     EKPVRYLAHVWNLANRIFSSFITGQCIEAVILGSMFFVSMTILHFPYAMLVGVLISFT
                     ALIPLFGGIIGCWVAFFLILMISPVKAVLFLGLFLILQQIEGNLIYPHVVGGSVGLPS
                     IWVLVAVTLGGSLMGIAGMLIFIPTVSVIYTLFREWVYARLEKKQLVL"
BASE COUNT         1278 a          890 c          763 g         1221 t
ORIGIN      
        1 agatcacatc cacttcatca gctaatgctt ccatattatc atccatacat ttcgcatcta
       61 ccagcttaaa aaagttctga taaacatctg catatttctg gtcaatataa cttcttgaac
      121 cataccacgc aacttccaca tctttatgtc ccagaagcag tctcacgatc tcgttacctg
      181 catatcctgt ggcaccgata attcctgctt taatcataat aatttcctcc atcttcgcct
      241 catccggcga tcttcatttc atagtaaaac tttactctat cagagtgctg gactaattat
      301 acatcttacc tgcataatat gcaatagtca tttttcaatg tttttacatg gattttattt
      361 cagttcacgg atttccccct gtcttttcct atattatgtt gcatatttat gcaaaaacag
      421 ataaaacttt tttgcatttt tctattgcat atttataaaa aaaggtgtat attatcactt
      481 gttaagcgga atcacacgaa atcatcttgt tccgcattga atatttattc aaaaatttca
      541 atcagaaatt accgcattta ttcaaaaata atgcgctgaa ctcccgacct attcatttac
      601 tttcggcaaa cggatatctc aggaaatttt tacaaatcag gaaaataata aaatccaatt
      661 tcaggaggaa tacaatcatg aaagaaaaag tcgttttagc atactcaggc ggtcttgata
      721 ccacagcctt aatcccatgg ttaaaagaaa catttgatta cgatgttgtc tgctgctgcg
      781 taaactgcgg tcagggaaac gaactggacg gactggatga aagagcaaag ctttccggtg
      841 cttccaaact atatatcgaa gatatcgtag atgaattctg tgatgacttc attgttccat
      901 gtgtacaggc aggtgctgta tacgaacaca aatatctcct tggtacatcc atggcccgtc
      961 cggcaatcgc taagaaatta gttgagattg cacgtaaaga aggcgcagtt gccatctgcc
     1021 acggtgctac aggtaaaggt aatgaccaga tccgtttcga acttggcatc aaagctcttg
     1081 ctcctgacat caagatcatc gctccatggc gtatgacaga caaatggacc atgcagtcac
     1141 gtgaagatga gatcgcattc tgtaaagctc acggaatcga ccttccattc gatgcaagcc
     1201 acagctacag ccgtgacaga aacttatggc atatcagcca tgaaggtctg gaactggaag
     1261 atccttcaca ggctccaaat tatgacaaca tgcttgtttt aggtgttaca cctgaaaaag
     1321 ctcctgataa agatactgag atcacaatga cattcgagca gggcgttccg aaaacattaa
     1381 acggcaagga aatgaaggtt tctgagatca tcactgagct taacaaatta ggcggcgaga
     1441 acggtatcgg taccgttgat atcgttgaga accgtgttgt tggtatgaaa tcacgtggcg
     1501 tatatgagac tcctggtgga actatcctga tggcagctca cgaacagctc gaagaactta
     1561 cacttgaccg tgaaacaatg gaaaccaaga agaaacttgg tagccagttc gctcaggttg
     1621 tttacgaagg aaaatggtat acacctctgc gtgaagcaat ccaggctttc gttgaatcca
     1681 cacagaaata tgtaacaggc gaagttaaga tgaaactgta caaaggcaat atcatcaaag
     1741 ccggtacaac ttctccatac agcctgtaca atgaatctct tgcatctttc acaacaggtg
     1801 acctctatga ccaccacgat gcagatggat tcatcacact gttcggactt ccactgaaag
     1861 ttcgcgctat gatgttaaaa gaagttgagc agaacaagaa ataattaatt tataacagac
     1921 agtttcagcc agatggacac tttattcatc tggctgtttt tattacctga tacagtattg
     1981 caattctgta aaaaacggca caaaaaaaga cacagccaga tggccatgtc cgtttttatc
     2041 tgctatgttt atgattatct gctttccagt tccgcacgtt tcttatcgat aagctcctga
     2101 agtttttcca gctcttccac tgtaatccca tcagattcca gaagcgcatt catcatttcc
     2161 accggattaa tctttctgct tttggcattt ccctttaaat aatcctctct tgaaagaaga
     2221 ggggtataat ttcttgcata caccttgccg ttcattgaag ttcccgcaac ctggatcagg
     2281 ttctctttca aaagaagctc caggcatctt cttactgtcg gcattttcag ttccggtgca
     2341 atgtcattga tctcaaaagc agacaatggt cttccctctt cccaaagcac attcataata
     2401 tcataacgtt taccttttaa gatgtccatc ttaagatctc cttccatttt taaccagata
     2461 tcactataat acagaaatat cgaattaatt acaattacag agaccttaca ctgatttaca
     2521 gtccttttac aaatttgtta ccaagtttat ccggacaata atacatcttt ctacacatca
     2581 cgaatcattt cgtcataaaa caagctgttt tttctcaaga cgggcatata cccactctct
     2641 gaaaagtgta tagatcacag atactgtcgg aatgaagatc agcattcccg caattcccat
     2701 cagactgccg ccaagtgtta cagctaccag tacccagatg gatggaagcc cgacagaacc
     2761 acctaccaca tgaggataga tcaggtttcc ttctatctgc tgcaggataa ggaaaagtcc
     2821 cagaaacaga actgctttta caggacttat cataagaatc agaaaaaatg ctacccagca
     2881 tccgataatt ccgccaaaca gcgggatcaa tgctgtaaat gaaatcagga cacccaccag
     2941 cattgcatat ggaaaatgca ggattgtcat ggatacaaag aacatacttc caagaatcac
     3001 agcttcgata cactgtccgg taataaagct ggaaaaaatc ctgtttgcca gattccatac
     3061 atgtgccaga tatctgaccg gtttttccgg gaacattgcg tacattgctt tttttacctg
     3121 ctgcataagt ttttctttct gcaaaaggac gtagcaggca aatacaaagc cgatggatac
     3181 attcattgca gtagtcagaa gtcccatggt gatggacact gtggaggaaa caatgttatt
     3241 gactccgttc ttcagtacgc cgagtacaga atcgattatt tttcccggct caatgtcaag
     3301 agactgcgcc catttcacaa cttccggatt gtttccgaat acattgtcga tccatttctg
     3361 gaagaccgga atgttttctt ctatcttttt cgctatattt acgaatgttg cccccagttc
     3421 cggtactact accagcataa cgatcacgat tatacaaatc acaaagcaga tagaaagcac
     3481 caggctgacc ggccttgcca gtttttgtgc tgtttttttc tcttgtttca aggcttttcc
     3541 gaacagatga tgttctatga atttcattgg aatattgagg ataaaagcga ttccgaaacc
     3601 aatgatgaac ggaaagagaa agccaagcag aactctgaca gtgctgataa ctatgtgcag
     3661 gttctggaca cccagatata agagaattgc gaatgcgatg agccatcgaa ttttttttat
     3721 gttttctttg tctagctgca tgtttgtttt tcctttcttt ttgatattgg agagtattat
     3781 atcatatttt aataataaaa tgagggacgc ttagaaaaat attttatctg ctatgcggtt
     3841 tcggcatcta agagattcta agtcatctga aatctgctaa aagcatgaga agaaaagtct
     3901 atgattgctc gctggtgctc aggcaatgga cttttcttct cataacgcag atttaagagg
     3961 actaagaatc tctaagatgc ctgcaaatgc atagcagata aaatattttt ctaagctggt
     4021 aattggaagg tggaaaatat cgtatcaatt cgcatgttaa aagcctacat gaataagaaa
     4081 gactttaaat atgaaagaga ttgtataagt tcacatggga tatcatatac tttatataaa
     4141 ttctgcctgt aa
//
LOCUS       DBMDXG010000162         4147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5084_length_4147_cov_3.167573, whole genome shotgun sequence.
ACCESSION   DBMDXG010000162 DBMDXG010000000
VERSION     DBMDXG010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5084_length_4147_cov_3.167573"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            106..330
                     /locus_tag="ACZPD3_05575"
     CDS             106..330
                     /locus_tag="ACZPD3_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HIR1415506.1"
                     /translation="MSEKTEITFMQTRLIRLASEEWHLPVEQIIHLFKEADVLGYIEK
                     CYGIFHCEGDEAVLEDITEFLQRKGIEISA"
     gene            323..889
                     /locus_tag="ACZPD3_05580"
     CDS             323..889
                     /locus_tag="ACZPD3_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009429487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HIR1415507.1"
                     /translation="MLELKDGFVLYHGSYCEVKEPDLAKCAKRKDFGQGFYLTTSKEQ
                     AESFLRTSIAKAIATGTIEEGQKFGYISTFEFKLSENLETHIFENADVDWLHCIAAHR
                     KKKMFIEVEREMAKYDIIAGKIADDATNATLTAYLAGAFGTAGDKVADDFCIRQLLPN
                     KLKDQYCFKTEAAIECLKFVKGEKIWLK"
     gene            877..1107
                     /locus_tag="ACZPD3_05585"
     CDS             877..1107
                     /locus_tag="ACZPD3_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415508.1"
                     /translation="MAEVTIPKEKMNYTIDLLITMVTDEIAEETGKDRNEVLTDFLCS
                     KTGKALYDEKTKLWCTGPAYIAELYMEELKKS"
     gene            1308..1487
                     /locus_tag="ACZPD3_05590"
     CDS             1308..1487
                     /locus_tag="ACZPD3_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415509.1"
                     /translation="MSRAKNPKYESMSLEELKANMEKSRQELEAAIHHKNILEQRKKR
                     KRPIRGCIEISGCCM"
     gene            1552..1950
                     /gene="tnpA"
                     /locus_tag="ACZPD3_05595"
     CDS             1552..1950
                     /gene="tnpA"
                     /locus_tag="ACZPD3_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpA"
                     /protein_id="HIR1415510.1"
                     /translation="MDLSSLTPDKQVKLQYWLDVIRQCRASGLTNQIWCEQHDISLKS
                     YYYWLSKIRKLALEELPRKKNGIHMCTDQQSTALTESPAEFAELSLPDRNISRSTPAA
                     ILHIGSITVELYEGTSAQMLENILKAVQSC"
     gene            1965..2315
                     /gene="tnpB"
                     /locus_tag="ACZPD3_05600"
     CDS             1965..2315
                     /gene="tnpB"
                     /locus_tag="ACZPD3_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017528.6"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HIR1415511.1"
                     /translation="MEKIYIRCGYTDMRKQLNGLLDIIQYNFKLDPYSNSLFLFCGKR
                     ADRIKAVHYEGDGFCLLYKRYENGRLQWPRTGEEAKQISDQQLRWLLEGLNPEQPKAV
                     QKWLPHKSENTENP"
     gene            2382..3917
                     /locus_tag="ACZPD3_05605"
     CDS             2382..3917
                     /locus_tag="ACZPD3_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISRob1 family transposase"
                     /protein_id="HIR1415512.1"
                     /translation="MSATEQEYKNHIKELEQQVRLLKEQVDFLTRKLYGTKSEKTSTL
                     EIEGQMSLFNEIETCADPDAHEPELVEIEKHLRKRKYTGQREELVKNLPHSKVLHTID
                     EREQICDNCGSTMVKVGEEFVRTEVQFIPAKLKVIDHYRETYECRSCRKNGTPYMEKA
                     PVPYPPVLHSLASASTITWLIHQKFELSIPLYRQEKEWEALGLRLSRATMSNWLLVVY
                     RDWLVHIVHRLKLELLKQRYLHIDETHVQVLKEPGRKNTSDSYMWVYCSIRDAVNPIR
                     YFEYQPGRSGKYPEAFLREYEGYIHTDAYSGYNAVSGVKRCLCYTHLRRAFVDALPKD
                     IHNSEASKPAEAILRLNQLFNIESELEALPPDQKKKERLIREKPLLEAFWSWAEKSAI
                     GELPKSKLSKAFHYALNNREGFWNYLEDGNCSISNSLAENCIRPFVIGRKNWLFSGSP
                     KGAEASAGIYTLVETAKANGLAPMKYIKYILSDMPGSAFLEHPEYLDDYLPWNPLVKE
                     FCR"
BASE COUNT         1301 a          820 c          971 g         1055 t
ORIGIN      
        1 tacaagggga gtttcccctt gcggcacagg tgcctatccc aataaagttt atgactgaat
       61 atttcagata cagacatata aagttataaa tttggaggat tcattatgag tgagaaaaca
      121 gagattactt ttatgcaaac aagactaatc cggcttgctt cagaagagtg gcatttacct
      181 gttgaacaga ttatccactt gtttaaagaa gccgatgttc ttggatatat agaaaaatgc
      241 tatgggattt ttcattgtga aggcgatgaa gctgtgcttg aagatattac tgaatttttg
      301 caaagaaagg ggatagagat tagtgcttga gttaaaagat ggatttgttc tctatcatgg
      361 aagttattgt gaagtaaaag agccagatct tgccaaatgt gcaaagagaa aagattttgg
      421 acagggattt tatttaacca catcaaagga acaggcagaa agttttctga gaacctcaat
      481 agccaaagcc atcgcaaccg gaacaattga agaggggcag aagtttggat atatttcaac
      541 ttttgaattc aaactttcag aaaatcttga aacgcatatt tttgaaaatg cagatgtgga
      601 ctggttacat tgtatcgccg ctcaccgaaa gaaaaaaatg tttattgagg ttgaacgtga
      661 aatggcaaaa tatgatatca ttgcaggaaa gattgcggat gatgcaacaa acgctactct
      721 taccgcttat cttgcgggtg cttttgggac tgcaggtgat aaagtagcag acgatttctg
      781 tatcagacag ttgctgccga ataaattgaa agatcaatac tgctttaaaa ctgaggcagc
      841 tattgagtgt ctgaaatttg tgaaaggtga gaagatatgg ctgaagtaac aattccaaag
      901 gaaaaaatga attatacaat cgaccttctg atcactatgg tgacagatga aatcgcagaa
      961 gagacaggca aggacagaaa cgaggtattg acagattttt tgtgttcaaa gacaggaaaa
     1021 gcactgtatg atgagaagac aaagctgtgg tgcactggtc cggcatatat agctgaactg
     1081 tatatggaag aattaaagaa atcctgatat gggatgtgag tttaaattta tacgacaatg
     1141 gtgacaaaga ctttcattcc aaggagcaac aattaaatat ttcaaaaacg ctgaggtcag
     1201 catttcctaa tcacaggaca acgacctcag ttttttatac ccaggaatcg gcattcatga
     1261 ataagccggt tctttttgtt tacagaaaaa agaaggagta caagaaaatg agcagagcaa
     1321 aaaatccaaa gtatgaatcc atgtcattgg aggaactgaa ggccaacatg gaaaagagca
     1381 gacaggaact ggaggcagca attcaccaca aaaacattct ggagcagcgg aagaagcgta
     1441 agcgtccaat aagagggtgt atcgaaattt ctggctgttg catgtaaact attgattaga
     1501 gttttgggag ttgactctaa aaactctaaa tctaatctaa ggagcaggct tatggatctt
     1561 tcttctttga ctccagacaa acaggtgaaa cttcaatact ggctggatgt aatacggcaa
     1621 tgcagggcct ccggactgac aaatcagatc tggtgtgaac aacacgatat ctctttgaaa
     1681 agttattatt actggctttc caaaattcga aagcttgcct tggaagaact gccccgcaag
     1741 aaaaacggca tccacatgtg tacagaccaa cagtcaactg ctttaacgga atccccggct
     1801 gagtttgcgg agctttcact tccggacaga aacatttccc gttccactcc tgcagccata
     1861 cttcatatcg gatccataac agtggaactt tatgaaggca catctgccca gatgctggaa
     1921 aacattctga aagccgtgca gtcatgttag gagagctttc ccatgtggag aaaatctata
     1981 tccgatgcgg ctacaccgat atgaggaagc agctcaatgg cttgctggat atcatccagt
     2041 acaacttcaa actggatcct tacagcaatt ccttattcct gttctgtgga aagagggctg
     2101 accggatcaa agcagtccat tacgagggag acggtttctg ccttttatac aaacggtacg
     2161 aaaacggacg tctccaatgg ccgcggaccg gcgaagaagc aaaacagatt tctgatcagc
     2221 agctgcgctg gcttctggaa ggtttgaacc cggagcagcc aaaggcggtt cagaaatggc
     2281 tcccgcataa gtctgaaaat actgaaaatc cttagaaatg ctgggaaatc tgacgattct
     2341 atggtataat aatcctatcg aagcgaaagg gttttcaggt tatgtcagct acggaacagg
     2401 agtataaaaa tcatatcaaa gaactggaac agcaggtccg gctcctgaaa gagcaggttg
     2461 atttcctgac ccgtaaactt tatggaacaa aatccgagaa gacatctact ctcgaaatcg
     2521 aggggcagat gtctcttttt aatgaaatcg aaacctgtgc ggatcctgat gcacacgaac
     2581 cggagcttgt tgagatagag aagcacctac gtaaaaggaa gtacaccggt cagcgggaag
     2641 aactggtaaa aaatctccct cacagcaaag tactccatac catagatgaa agggaacaga
     2701 tatgcgataa ctgcgggagt acgatggtga aagtagggga agaattcgtc cgcacggaag
     2761 tacagtttat ccctgcaaaa ctcaaggtga ttgaccatta ccgggaaacg tatgaatgca
     2821 ggtcgtgccg gaaaaacgga actccttata tggagaaggc tccggtgcca tatcctcccg
     2881 tcctgcattc tctggcatct gcatctacca tcacctggct catccaccag aagtttgaat
     2941 taagcatccc gctgtacaga caggaaaaag aatgggaggc tttgggatta cgtttaagca
     3001 gagcgactat gtcaaactgg cttctggttg tctatcggga ctggcttgta cacatcgtcc
     3061 accgtctgaa gctggaactt ctgaaacaga gataccttca tatcgatgaa acacatgtac
     3121 aggtgctgaa agaaccggga cggaaaaata cgtccgattc ctatatgtgg gtttactgca
     3181 gcatcagaga tgctgtaaat cccatccgct acttcgagta ccagccggga cgaagcggga
     3241 aatatccgga agcgtttctg agggaatatg aaggttatat ccatacagac gcttactccg
     3301 gatacaatgc agtttcagga gtaaaaagat gtctttgcta tacgcatctg cgccgggctt
     3361 tcgtggatgc gctcccaaaa gacatccata attcggaagc atcaaaacct gcggaagcaa
     3421 tcctccgtct gaatcaactc tttaacatag agtcagagtt ggaggcgctc cctcctgatc
     3481 agaagaaaaa agaacgtctt atccgcgaga aaccgcttct cgaggctttt tggtcatggg
     3541 cagaaaaaag tgccatcgga gagctgccga aatccaaact ttcaaaagct tttcattacg
     3601 ccttaaataa ccgtgaaggt ttttggaatt atctggaaga cgggaattgt tccatcagta
     3661 attcgcttgc ggaaaactgt atccgtccct ttgtgatcgg cagaaagaac tggctgttct
     3721 ccggcagtcc gaaaggggcg gaagccagtg caggaatcta tacactggta gagacagcga
     3781 aagcgaatgg gcttgccccg atgaaatata taaaatatat cctgtccgac atgccgggga
     3841 gtgcttttct tgaacatcct gaatatctgg atgattatct gccttggaat cctcttgtaa
     3901 aagaattctg tcgataaccg ttgacctttt agtataaaag gctgacggtt atttttctat
     3961 acactgtttt atttgacact tacgttattt ttttgcccat tttgaattca tcacttgaca
     4021 ttgtggcaaa gagctgatga gccaggacga gctggcagtt atggatggcg gcaagtgcat
     4081 cttgcagttg cgcggtgtgc gtccgtttct gtcagacaag tattacatta ccagacaccc
     4141 taatttc
//
LOCUS       DBMDXG010000163         4142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5093_length_4142_cov_1.700322, whole genome shotgun sequence.
ACCESSION   DBMDXG010000163 DBMDXG010000000
VERSION     DBMDXG010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5093_length_4142_cov_1.700322"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            84..599
                     /locus_tag="ACZPD3_05610"
     CDS             84..599
                     /locus_tag="ACZPD3_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024770.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1415513.1"
                     /translation="MSLIFFILERGHRKIAYIHGEDSSVTRNRLGSFYRTLQKKRIDI
                     PDEYMPVIPYRDAEAAAIATSELLDLKDPPTCILYPDDFSALGGINEIHERGLRIPDD
                     ISIAGYDGLTFARILEPRLTTLCQDTETIGRLAAQKLIDLIENPKTTIIDKFTVDGRL
                     FPGASVRNLAK"
     gene            686..2014
                     /locus_tag="ACZPD3_05615"
     CDS             686..2014
                     /locus_tag="ACZPD3_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1415514.1"
                     /translation="MKRKAMAITLTAAMLAGLTAVPTWAEDAAADEGKVLNIYCWNEE
                     FKSRLTDHYPGYEEVDGTHGKIGDVDVVWNITPSDDNAYQNNLDETLLKQADAAADDK
                     IDLFLVEADYALKYVNTDYTMSVADLGITDEEVADQYQYTKDVMTDSDGNLKGLSWQG
                     CPGAMIYRRDIAKEVFGSDDPAEVQKKVADWDTFKATAEELKEKGYQMTSTVNDAYRV
                     FSNNVTSPWVVDGKITVDDNIKNWVDMSKEMVDAGETATYELWSDDWSKGFFKDSNVF
                     SYFGPAWFIDFSMSADQDGSVGKDGGWAATEGPQGFYWGGTWITAATGTDNPTLVADI
                     MRTMTTNVDVMKEIVTADNDFVNNKPAMEEMAKDESYGDAVLGGQNPLAMFCAGADKI
                     DLSNMSIYDQGCNEEFQNAMKNYFEGNASYDEALDLFYKAVVEKYPELSY"
     gene            2093..3001
                     /locus_tag="ACZPD3_05620"
     CDS             2093..3001
                     /locus_tag="ACZPD3_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415515.1"
                     /translation="MAKNNNHKAVSYNKWGYIFLIPFVVVYLIFQLVPLISTIYNSFF
                     ENYRSGLTQIGPNFVGLANYAKLFQGGDIWIYFKNTMIMWVMGFIPQILVSLLLAAWF
                     SNPSLRLKGQRFFKTVIYLPNLIMASAFAMLFFTLFADGGPINAMLMQSGILKQAYSF
                     FSHAWSARTLVAVLNFLMWFGNTTILLMAGMMGIDSSLYEAAEVDGATSSQIFRKITL
                     PLLRPILIYVMITSLIGGMQMFDVPQILTNGTGDPMRSTMTLIMFLNKHLFSKNYGMG
                     GALSVFLFIITGVLSLIVFRFNKIKD"
     gene            3016..3879
                     /locus_tag="ACZPD3_05625"
     CDS             3016..3879
                     /locus_tag="ACZPD3_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415516.1"
                     /translation="MNEKNSKNKKGMSMKGQRTLAYIVLIIISFFCLFWFYVLFINAT
                     RSNGALKKGFTAIPGGHLIENWKNLLAGTLPVWNGMFNSIFIATCSAVLCTYFSTMTA
                     YAIHAYNFKAKKFMFTFILAVMMIPTQVTALGFLQLLGKIGLDDSFVPLIVPSIAAPV
                     TFFYMKQYMESNLPLELVEAARIDGSGEFNTFNKIVFPLMKPAIAVQAIFTFVQSWNN
                     YFIPALVLHSDKKKTLPVLIAQLRSADFLKFDMGQVYVMIAFSIFPVIIVYILLSRFI
                     VEGVSAGAVKG"
BASE COUNT         1194 a          904 c          883 g         1161 t
ORIGIN      
        1 cgagataccg gtagtcacta tcgatcatgt atttgatgga agaattgctg ttgtttccaa
       61 caacattcag ggaatggaag aacttgtctc ttatattctt tattcttgaa cggggacaca
      121 gaaagattgc ctatattcac ggagaagatt cctctgtcac acgaaacaga cttggaagtt
      181 tttacagaac tctccagaaa aaacgaatcg atatacctga tgaatatatg ccagtcattc
      241 cttatcgaga tgcagaagct gcagcaatag caacttcaga gcttctggat ctgaaagatc
      301 ctcctacctg tatcctctat ccggatgact tctccgcact gggtggcatc aatgaaatcc
      361 acgaacgtgg acttcgaata ccagatgata tttccattgc aggctatgac ggacttactt
      421 ttgcacgcat cctggagcca cggctgacaa ctctgtgtca ggacacagaa acgattggac
      481 ggcttgccgc acagaagctg attgatctta tcgaaaatcc taaaacaacg atcatcgata
      541 aattcaccgt tgatggaaga cttttccccg gtgcatcagt cagaaatctg gcaaaataag
      601 aaaatcttca gtatcttcac agttggtgga ctgctgaaaa gttacaatca acccaaaaca
      661 attatcacaa ggaggtaata acatgatgaa gagaaaagct atggcaatta cattaacagc
      721 agctatgctt gccggtctta cagcagttcc aacatgggct gaagatgcag ccgctgacga
      781 aggtaaagtt ttaaacattt attgctggaa tgaagaattc aaatcccgtc tgactgacca
      841 ctaccccgga tatgaagaag ttgacggaac tcatggcaag atcggagatg ttgatgttgt
      901 atggaacatt actccttctg atgacaatgc atatcagaat aatctggatg agactcttct
      961 gaaacaggct gatgcagcag cagatgacaa aatcgatctc ttccttgttg aagccgatta
     1021 tgcactgaaa tatgtaaata cagattatac aatgtctgtg gcagatctgg gaatcacaga
     1081 tgaggaagtt gcagaccagt atcagtacac caaagatgtt atgacagatt cggatggcaa
     1141 cttaaaaggt ctttcctggc agggctgccc gggtgctatg atctacagaa gagatattgc
     1201 aaaagaagta ttcggaagcg atgatccggc agaggttcag aagaaagttg cagactggga
     1261 tacatttaag gcaacagctg aagagctgaa ggaaaaaggc tatcagatga cttccactgt
     1321 aaatgatgcc taccgtgtat tctctaataa tgtaacttcc ccatgggttg ttgacggaaa
     1381 gatcactgtt gatgacaata tcaaaaactg ggtagatatg tccaaggaaa tggttgatgc
     1441 cggagaaaca gctacctatg aattgtggag tgatgactgg agcaagggct tcttcaaaga
     1501 cagcaatgta ttctcatatt tcggacctgc atggttcatc gacttctcca tgagcgctga
     1561 ccaggatggt tcagttggta aagatggcgg ctgggctgca acagaagggc cacagggctt
     1621 ctactggggc ggaacatgga tcacagcagc tacaggcaca gacaatccta cactggttgc
     1681 tgatatcatg agaactatga caactaacgt tgacgttatg aaagaaatcg ttactgctga
     1741 taatgacttc gtaaacaata aacctgccat ggaagaaatg gctaaagacg agagctacgg
     1801 cgatgcagtt ctcggcggac agaatcctct ggctatgttc tgtgcaggcg ctgataagat
     1861 cgacctgagc aacatgagca tctatgatca gggctgcaac gaagaattcc agaatgccat
     1921 gaagaactac ttcgaaggca acgcatctta tgatgaagct ctcgacctgt tctataaggc
     1981 agttgtagaa aaatatccgg aactttccta ttaatcaaac attgaaacct gtgtgcctgc
     2041 ctgaccatca ggcaggcaca ctttggatta cagcagacta tggaggaaga taatggcaaa
     2101 gaataataac cataaagcgg tcagctataa caaatgggga tatatcttcc tgataccatt
     2161 tgtagtagta tatctcatat tccagctcgt tccgcttatc agtacaattt ataacagttt
     2221 ctttgaaaat tacagatctg gattaacgca gataggaccg aattttgtgg gacttgccaa
     2281 ttatgcaaag ctctttcagg gtggcgatat ctggatttat tttaaaaaca caatgatcat
     2341 gtgggtaatg ggatttatcc cacagatcct ggtttccctg ctcctggctg cctggttctc
     2401 caatccaagc ctccggctta agggacagcg cttcttcaaa acagttattt atctgccaaa
     2461 cctgatcatg gcatccgcct ttgcaatgtt attctttacc ttatttgcag acggcggtcc
     2521 catcaatgca atgctgatgc agtcaggaat actgaaacag gcatattcct ttttcagcca
     2581 tgcctggagt gccagaaccc ttgttgcagt cctgaacttc ctgatgtggt tcggaaatac
     2641 aactattctt cttatggcag gtatgatggg aattgactcc tcgctttacg aggcagctga
     2701 agtagacggt gcaacttcct cacagatttt cagaaagatc actcttccac tccttcgtcc
     2761 aatcctgatc tatgttatga tcacatccct gatcggcggc atgcagatgt tcgatgtacc
     2821 tcagatcctg acaaacggaa ccggcgaccc catgcgttcc actatgacac tgatcatgtt
     2881 cctgaacaag catctgttca gcaagaacta cggaatgggt ggtgcattat ccgtattcct
     2941 gttcattatc accggagtgc tcagcctgat cgtattccgc tttaacaaga taaaagactg
     3001 atggaggtga gaagaatgaa tgaaaaaaac tccaaaaata aaaaaggaat gagcatgaaa
     3061 ggtcagcgta cccttgccta tatcgtactg atcatcattt ccttcttctg cctgttctgg
     3121 ttttatgtac tgtttatcaa tgccaccaga tctaatggcg cactgaaaaa gggattcaca
     3181 gccattcccg gaggacatct cattgagaac tggaaaaatc ttctcgcagg aacactccct
     3241 gtatggaatg gtatgttcaa cagtattttc attgctacct gctccgctgt tttatgtacc
     3301 tatttttcaa caatgactgc ttacgctatc catgcataca attttaaagc aaaaaaattt
     3361 atgtttacat ttatccttgc agtcatgatg atcccaactc aggtaactgc tcttggattc
     3421 cttcagctgt tgggaaaaat aggtctggac gactcctttg tcccactgat cgttccaagt
     3481 attgctgctc ctgttacttt cttctatatg aaacagtaca tggaaagcaa ccttcctctg
     3541 gaacttgtgg aagcagcacg tattgacggt tccggtgaat ttaatacctt taacaaaatc
     3601 gtattcccgc ttatgaaacc ggctatcgct gtgcaggcaa tttttacctt tgtacagagc
     3661 tggaataatt acttcattcc ggcgctggtt cttcactcag ataagaaaaa aacacttcct
     3721 gtactgatcg cacagcttcg aagtgcagac ttccttaagt ttgacatggg acaggtgtat
     3781 gtaatgatcg ccttttccat cttcccggtc atcattgtct acattcttct ttccagattc
     3841 attgtagaag gtgtttccgc aggtgcggtt aagggttaga tgctgataca acaaaagctg
     3901 tctgttccgg atgcaaatac atccggaatc cagacagctt ttttaattat ttgcagtact
     3961 gttcggactt ccgaccacct tcacttcgtt tgccatcttt attttccttc cttattctgc
     4021 ttctttcagt tccttactca gctctgacgc aaagtatttt atactattcg catccagtcc
     4081 aaaggctttc aatattactt tctggttcgc tgttactgca tgatccagac ggtatatatt
     4141 gt
//
LOCUS       DBMDXG010000164         4104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5140_length_4104_cov_1.801399, whole genome shotgun sequence.
ACCESSION   DBMDXG010000164 DBMDXG010000000
VERSION     DBMDXG010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5140_length_4104_cov_1.801399"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="ACZPD3_05630"
     CDS             <1..289
                     /locus_tag="ACZPD3_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRSP domain-containing protein"
                     /protein_id="HIR1415517.1"
                     /translation="ALYIGRKMEKEGAEVRCHSTTRSPIAVSLEKEYPLHSRYELKSL
                     YDPDRRTFIYDIGKYDKVLIVTDSPEIKESQETLINAVRMQNKDITVVRWC"
     gene            291..1343
                     /locus_tag="ACZPD3_05635"
     CDS             291..1343
                     /locus_tag="ACZPD3_05635"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HIR1415518.1"
                     /translation="MRSSYSEEDVILLLKDITGLVEPQPAKVREKLIQSGKHYSEMLP
                     VEYVPTDQYMQVYHNALKHYAKPVANAVGMLADKIIENKGKKMVLVSLARAGIPIGIL
                     VKRYIKFKYGINVPHYSISIIRGRGIDDNAMKYLLEKYRPQQILFVDGWIGKGAILNE
                     LKKDISAYEGVSADIAVVADPANVTELCGTHEDILIPSSCLNSTVSGLISRTFLRDDI
                     IGRDDFHGAVYYGELTDSDLSYDFIETIEKEFNMENTPDLEKRVESQGIDEVINIGKN
                     FGIDDINLIKPGIGETTRVLLRRIPWKVLIDERYKGNPQLEHIVRLAEEKHTPVEYYP
                     LTHYKCCGIIKKLADA"
     gene            complement(1356..2318)
                     /locus_tag="ACZPD3_05640"
     CDS             complement(1356..2318)
                     /locus_tag="ACZPD3_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR1415519.1"
                     /translation="MKNNVLYYSVGPLLYCPANRISITDSLINERFGNRFSLALCLED
                     TINDDHVEEAEQILISSLSQIFIQHEQKPFYLPKIFIRVRNPQQIQRLTKALGQSIKI
                     VTGFIVPKFSPDNAQSYIEQMILVNELVAKKLYMMPIYESPSIIDLRNRIDILYLLRD
                     SLARIEDLILNIRVGGNDLCHMFGFRRHANESIHSIRPVSDIFSDIITVYGMDYVISG
                     PVWEYYAGDSWKEGMIQEIREDRLCGFIGKTVIHPSQIPVVNRAYQVSRNDYLDARAI
                     LNWNADSASLVAGSKTRERMNEYKTHLNWAKKTVYLSEVFGITE"
     gene            2665..>4104
                     /locus_tag="ACZPD3_05645"
     CDS             2665..>4104
                     /locus_tag="ACZPD3_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HIR1415520.1"
                     /translation="MNSRREKGVYFYRINGYSEEVGEFIKKYYDTARRTGVVIEGKIP
                     NPDEGNLAYYNEIMGMDFQMSMDFIHVSLRKWLPRMNEFQRQNVAASIYDSLDSLRKA
                     GKTENMLRNAYIKFMCWLYYKFERIVNQLGENHIPKILYEGQISNYELMLISILSNAG
                     CDVVLLQYAGDQGYLKTDPGSVLSDSLQMEGLQPFPQGYCVKKVRDEIQNELNNERLY
                     GIRPSLTNCTNAWIKGNGLDDIRESILLRGNDSRFFYNCFCRINGAEDKLTYANELFR
                     LQQELRNSKRNTVIVSKEIPRPTPQEISEIKRSNYTSGDQMLLGLACNIQYGANPELQ
                     RILHKTFVDVMLAESQKEGENLNRLTNRAVYLLCWMRRYLPKLFINWKSPEIGCFIYL
                     GGCRNENEALFMSFLGRLPLDVLILCPDLNIKCCLEDKLLYEVNYPESLAITEYPEES
                     SQVKIGTAAYHAERELDTLMYQDSGIYRNQ"
BASE COUNT         1321 a          703 c          964 g         1116 t
ORIGIN      
        1 tgcactttat atcggaagaa agatggaaaa agagggagcc gaagtaaggt gccattcgac
       61 tacaagaagt cccattgctg tgagtctgga aaaagagtat ccccttcaca gcagatatga
      121 actgaaaagt ttatatgatc cggacaggag aacgtttatt tacgatatag gaaaatatga
      181 caaggtattg attgtcacag attccccgga aataaaggag agtcaggaaa cgctgataaa
      241 tgctgtccgg atgcagaaca aggatattac agttgtaagg tggtgctgat ttgagaagtt
      301 cctattcaga agaagatgtg attttattat tgaaggatat taccgggttg gtagaacctc
      361 agcctgccaa agtgcgggag aaactgattc agtccggaaa acattacagc gagatgctgc
      421 ctgttgaata tgttcctaca gatcagtata tgcaggtata tcataatgcg ctgaaacatt
      481 atgcgaagcc ggttgccaat gcagtaggaa tgctggcaga taagatcata gagaataagg
      541 gaaagaaaat ggtcctggta tcccttgcca gagcgggaat cccaattggt atccttgtga
      601 agcgatacat caaattcaaa tatggtataa atgtgcctca ttattccatt tcaattatca
      661 gaggaagagg gattgacgat aatgccatga agtatctgct tgaaaagtac agaccgcagc
      721 agattttatt tgtggacgga tggattggca agggtgcgat cctgaatgaa ttgaagaaag
      781 atatctctgc atatgaaggg gtttctgctg acatagcggt agtggcagat ccggcaaatg
      841 ttacggagct ttgcggaaca catgaagata tcctgatccc aagttcctgc ctgaacagta
      901 cagtatccgg ccttatcagc cgtacttttc tgagagatga cataataggc agagatgatt
      961 tccatggggc agtctattac ggagaactga cagattctga tctgtcatat gactttattg
     1021 aaaccattga aaaggaattt aatatggaga atacacctga tctggaaaaa agagtggaaa
     1081 gtcagggtat tgatgaggta ataaatatag gaaagaattt cggtattgac gatattaatc
     1141 tgatcaaacc ggggatcgga gagacaacaa gagtattact gaggagaatt ccctggaagg
     1201 tgctgatcga tgagcgatat aagggaaatc ctcagttgga gcatattgtc agacttgccg
     1261 aagagaaaca tacacctgta gaatactatc cacttacaca ttataaatgc tgtggaatca
     1321 ttaagaagct tgcggatgcg tgagaagggt aatacttatt ctgttattcc gaaaacttct
     1381 gaaagataaa ctgttttctt agcccagttc agatgggttt tatattcatt cattctttct
     1441 ctggttttgc ttcctgcaac aagggaagca gaatcagcat tccagtttaa aatcgcccgt
     1501 gcgtcaaggt agtcatttcg tgaaacctga taagctctgt ttactacagg gatctgtgaa
     1561 gggtggataa cggtttttcc gatgaaaccg caaagtctgt cctcccggat ttcctgaatc
     1621 attccttctt tccagctgtc accggcataa tattcccata caggaccgga aatcacataa
     1681 tccataccat atacagtgat aatatctgag aaaatatctg aaacaggtct gatgctgtga
     1741 atagattcat tggcatgtct gcgaaaccca aacatatggc acagatcatt tccgccaaca
     1801 cgtatattca atataagatc ttcaattctg gctaaagaat cccttaacaa atagagaata
     1861 tcaattctgt ttcgcaaatc tataatggag ggactttcgt aaataggcat catgtaaagc
     1921 ttttttgcga caagttcatt gacgaggatc atctgctcaa tataactttg tgcgttatcc
     1981 gggctgaatt tagggacgat gaaaccggta acaatcttaa tggattgccc aagagctttt
     2041 gttaatctct gaatctgctg tggattccga acccggataa atattttggg cagatagaat
     2101 ggcttttgtt catgctgtat gaatatctgg ctgagagaag aaatcagtat ctgctcggct
     2161 tcttcaacat gatcgtcgtt tatggtatct tcaagacaga gagcaagaga aaatctgttc
     2221 ccgaatcttt catttatcag agaatcagtg atggatattc tgttggcagg acaatagagt
     2281 aatggaccta cactgtaata taaaacatta tttttcataa cactccttct gacatatcta
     2341 caaaaattcc cgtaaatagg gtaccttata gaaattcaat aaaatactgc aggtaatccg
     2401 gtccatcaca gtgtgaagga ccgagtcagc agctacatca tagcacattt caggggaata
     2461 agccatagaa agaacagggg aattttcttg aaatataaat agcaatatga tacaattttg
     2521 aggtagaatt tatacgaaaa tttggagaaa attatggttc cgcattattt tatgaaatta
     2581 ctgtagaact tatcatgaac agaaataaga taagcagata ttgaaggtga gatacttata
     2641 tgttggaaca caaattttgt cgaactgaac agcaggcggg agaaaggtgt ttatttctac
     2701 cgtatcaacg gatattctga agaagtcggc gaatttataa agaaatatta tgatacagca
     2761 agaagaaccg gagtggttat cgaagggaag atacctaatc ccgatgaggg gaatcttgca
     2821 tattataatg aaataatggg aatggatttt cagatgagta tggattttat ccatgtcagt
     2881 ctgagaaaat ggctgccaag gatgaatgag tttcagagac agaatgtggc agcctccatt
     2941 tatgactctc tggattccct gcgaaaagcc gggaaaaccg agaatatgct caggaacgca
     3001 tatatcaagt tcatgtgctg gctgtattat aaatttgaac gcattgtaaa tcagcttgga
     3061 gagaaccata tccctaaaat attatatgaa gggcagataa gcaattatga gttaatgctc
     3121 atatcgattc tttccaatgc aggctgtgat gtagtactgc ttcagtatgc aggggatcag
     3181 ggatatctga aaacagatcc gggttccgtt ctttcagaca gtttgcagat ggaaggtttg
     3241 cagccatttc cgcagggata ttgtgtgaaa aaggtcagag atgagattca gaatgaactt
     3301 aacaatgaga gactgtatgg aatcagacct tcgcttacaa attgtacaaa tgcatggata
     3361 aagggaaacg gcctggatga tatccgcgag agcattctgt taaggggaaa tgacagccgt
     3421 tttttttata actgtttctg caggataaac ggggcagaag ataagcttac ttatgcaaat
     3481 gaactgttca gattgcagca ggaactgaga aattcaaaga gaaatacggt gatcgtaagt
     3541 aaagagattc caagaccaac gccgcaggaa atttcagaga taaagaggtc aaactacaca
     3601 tccggagatc agatgctcct gggacttgcg tgtaatatac agtatggagc caatccggag
     3661 cttcagcgga tcctgcataa aacatttgtg gatgttatgc tggctgaatc tcagaaggaa
     3721 ggggaaaatc ttaacagact gacaaacagg gcagtgtatc tgctgtgctg gatgagaaga
     3781 tatctgccga agctgttcat aaactggaaa tcaccggaga ttggatgctt tatttatctg
     3841 gggggctgcc ggaatgaaaa cgaagctctg tttatgagtt tccttgggcg gctgccactg
     3901 gatgtgctga tcttatgtcc tgatctgaac attaagtgct gtctggaaga taaacttctg
     3961 tatgaggtca actatccgga gagtctggcg atcacagaat atccggaaga gagttctcag
     4021 gtaaagattg gaacagccgc ttatcatgcg gaaagagaac ttgatactct gatgtatcag
     4081 gacagtggga tatacagaaa tcag
//
LOCUS       DBMDXG010000165         4103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5142_length_4103_cov_1.609445, whole genome shotgun sequence.
ACCESSION   DBMDXG010000165 DBMDXG010000000
VERSION     DBMDXG010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5142_length_4103_cov_1.609445"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(72..1661)
                     /locus_tag="ACZPD3_05650"
     CDS             complement(72..1661)
                     /locus_tag="ACZPD3_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR1415521.1"
                     /translation="MSKYTNEIEKRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSV
                     KGKATARHAVSDWMEIEKERGISVTSSVLQFHYDGYCINILDTPGHQDFSEDTYRTLM
                     AADSAVMVIDGSKGVEAQTRKLFKVCVMRHIPIFTFINKMDRDANDTFDLLDEIEKEL
                     GIATCPINWPIGSGKKFRGVYDRDTEKILTFSDTQKGTKEGVIEEIPLSDSRADEIMD
                     PEQKAQLLDEIELLDGASADFDQELVSKGKLTPVFFGSALTNFGVETFLEHFLKMTTS
                     PLPRVSDIGEIDPMDDDFSAFVFKIQANMNKAHRDRIAFMRICSGKFDASQEVYHVQG
                     DKKMRLLQPQQMMAESRHVVDEAYAGDIIGVFDPGIFSIGDTICAPGKKFAFEGIPTF
                     APEHFARVRQIDTMKRKQFVKGINQIAQEGAIQIFQEFNTGMEEIIVGVVGVLQFDVL
                     KYRLENEYNVDIRLETLPYEHIRWIANKEEVKVDKIIGTSDMKKVTDLKGNPLLLFVN
                     SWSVGMTEDRNPGLILTEFSK"
     gene            complement(1817..2569)
                     /locus_tag="ACZPD3_05655"
     CDS             complement(1817..2569)
                     /locus_tag="ACZPD3_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HIR1415522.1"
                     /translation="MKKVIGKNQVIITSLAILIAVAGYLNFADVDLGFRDKEASTDSS
                     SILEDAGYDLTDETALLDENQADGGLTDNSLTDSQETDTPGEAVFTGSTGFAAQAKIS
                     REQVRSQNKADLQDIINNEEIDDEEKQEAIHTMVSMTDLSEKEAAAELLLEAKGFKNV
                     VVNLTGETADVVIPEAELSDAQRAQIEDIVKRKTGIAPENIVITPLKESEDAEDTTQT
                     DETSADTSYEEDSETSAQPYEDTAIDTTDIYD"
     gene            complement(2580..2840)
                     /locus_tag="ACZPD3_05660"
     CDS             complement(2580..2840)
                     /locus_tag="ACZPD3_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415523.1"
                     /translation="MQKSVLEEKLEAFLENTEGVGKVQVILMTDEKKDRQSFYNSETI
                     QVTGVLISAEGGGNPVVVQNIQEAVMALFQVDAHRIRIMKMK"
     gene            complement(2881..3447)
                     /locus_tag="ACZPD3_05665"
     CDS             complement(2881..3447)
                     /locus_tag="ACZPD3_05665"
                     /inference="COORDINATES: protein motif:HMM:NF021121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HIR1415524.1"
                     /translation="MKEELYLWIRNLAVFYIFFTAVLNLIPDQKYEKYVRFFMGLLLI
                     FMMSTPIFSILGKGSELTESFLDNFSEENREKELREFQNIQKVYLEKGYELELEQKIR
                     ETLEKRGIEVYKVKVNIEGEETQANLVLKTEISQEERKELKDALVEEWGLKENRICIQ
                     IVRNESGKMGNPVAHRSTSGSSGDACIQ"
     gene            complement(3480..>4103)
                     /locus_tag="ACZPD3_05670"
     CDS             complement(3480..>4103)
                     /locus_tag="ACZPD3_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HIR1415525.1"
                     /translation="AASTGSSTAAAFYDGILLMIWAVQWLLANLFLPAVNLSLLLKMV
                     NYLSKEEMLTKMAELLDVAVNWGLKTLLGAIVGLQIVRNMVSPVMDAMKRSAVGKAAS
                     AIPGIGNAVTAVTELVLTSAVIVRNSFGAVIVILLLLIGAGPIIHYGSLSLVYRFLAA
                     IAQPISDKRVVGALSTMGEGCAMLLKLLLTAEVLCMLTFLIVVVSVS"
BASE COUNT         1062 a         1054 c          702 g         1285 t
ORIGIN      
        1 catctgaaaa cctcctgagg gattctttaa tctttgttca ttataccaaa tcgacagata
       61 aatgaaaagg gttatttact gaattccgtt aatattaagc caggatttct gtcctctgtc
      121 atacccacac tccaggaatt tacaaagagg agcagcggat ttcccttaag atcagtaact
      181 tttttcatat cagaggttcc aatgatctta tccactttta cttcttcttt atttgcaatc
      241 cagcgaatat gttcatatgg aagtgtttcc agacggatat ccacattata ttcattttcc
      301 agacgatatt tcagaacatc aaactgcagg acacctacaa cacctacaat aatttcttcc
      361 ataccggtat taaactcctg aaaaatctga atagcacctt cctgcgcgat ctgattgata
      421 cctttaacaa actgttttct cttcatagta tcaatctgac ggactctcgc aaaatgttcc
      481 ggtgcaaatg taggaatacc ttcgaatgca aactttttac cgggtgcaca gattgtatct
      541 ccgatggaaa agatacccgg gtcaaaaaca ccgataatat ctccggcata tgcctcatct
      601 accacatggc gggattctgc catcatctgc tgcggctgaa gcagacgcat ctttttatca
      661 ccctgcacat gatatacttc ctgggaagcg tcaaatttac cggaacagat tctcataaat
      721 gcgatcctgt ctctgtgtgc cttgttcatg tttgcctgga ttttaaatac aaacgctgag
      781 aaatcatcat ccatggggtc aatttcccca atatcagaca ctctcggaag cggtgatgtt
      841 gtcatcttca ggaaatgttc cagaaatgtt tccacaccga agtttgtcag cgcagatccg
      901 aagaatacag gtgtcagctt acctttgctt accagctcct ggtcaaaatc tgcacttgcg
      961 ccgtccagaa gctcaatctc atccagaagc tgtgctttct gctccggatc cataatctca
     1021 tctgcccggc tgtcacttaa cgggatttcc tcgatcacac cttctttggt acctttctgt
     1081 gtatcagaaa aggtaagtat tttctctgta tcacggtcat aaacaccgcg gaatttctta
     1141 ccggaaccga ttggccagtt gatcgggcag gttgcgatgc caagttcttt ctcaatctca
     1201 tcaagaaggt caaatgtgtc gtttgcatcg cggtccatct tattgataaa tgtaaaaatc
     1261 gggatatggc gcattacgca gaccttgaac agttttctgg tctgtgcctc cacacccttg
     1321 gaaccgtcga ttaccatgac tgcagaatct gctgccatca gcgtacggta agtatcctcc
     1381 gagaaatcct gatgtcccgg tgtatccaga atattaatgc agtaaccatc ataatgaaac
     1441 tgcagaacag aggaagtaac ggaaatacct ctctcttttt caatctccat ccagtctgac
     1501 actgcatggc gggctgttgc cttaccttta acacttccgg caagattgat tgctccgcca
     1561 tacagcagga atttctcagt cagtgtagtt ttacctgcat ccggatggga aataatggca
     1621 aatgttcttc ttttttctat ttcattcgta tacttagaca cgttcagcct cctgttgcgc
     1681 ttttttataa ttgtttgcat aaaacaccgg caacagccgg tatcagttac gttttctatt
     1741 tttttacagc attcctattc tagtatatgc acctcttttc tgtcaagata tatgttcgca
     1801 tattcatatc aaaatatcag tcataaatat cagttgtatc gattgcggta tcctcatatg
     1861 gctgtgctga tgtttcgctg tcttcctcat acgaagtatc tgctgatgtt tcatctgtct
     1921 gtgttgtatc ttctgcatct tcactctcct tcagtggggt gatcacaata ttttctggcg
     1981 caattcctgt tttccttttt acaatatctt caatctgtgc tctctgtgca tcagaaagtt
     2041 ctgcttcagg gatcacaaca tccgcagttt ctccagtaag atttacaact acattcttaa
     2101 agcccttcgc ctccagcaaa agctctgctg ccgcttcttt ttctgaaagg tctgtcatcg
     2161 agaccatcgt atgtattgcc tcctgcttct cttcatcatc aatttcttca ttattgataa
     2221 tgtcctgcag atctgccttg ttctgtgagc gcacctgttc tctgctgatc tttgcctgtg
     2281 cggcaaatcc tgtagaaccc gtaaacacag cctccccggg agtatccgtt tcctgactgt
     2341 ctgtaagact gttatcagtc agacctccat ctgcctgatt ttcatccaga agagcagttt
     2401 catctgtaag atcatatcct gcatcttcaa ggatactgct gctgtccgta cttgcctcct
     2461 tatccctgaa tccaagatct acatccgcaa agttcagata tcctgccact gcaatcagaa
     2521 ttgccagcga tgtaataatg acctgatttt ttccgatgac ttttttcaca tttatctcct
     2581 catttcattt tcattattct aattctatga gcatccacct gaaataatgc catgaccgcc
     2641 tcctgaatat tttggaccac tacaggattt cctccacctt ccgcagaaat cagaacaccg
     2701 gtcacctgta tggtttctga attatagaag ctctgcctgt ctttcttctc atctgtcatc
     2761 aggataacct gtacctttcc caccccttcc gtattttcaa gaaatgcttc aagtttctct
     2821 tccagaacac ttttttgcat atcttcactc tgctctcctg ccgtattttt cttttgctgc
     2881 ttactggata caggcatcgc cgctactgcc agaagtactc cgatgagcaa caggattccc
     2941 cattttgccg gattcatttc ggacaatctg gatacaaatt ctgttttctt taagtcccca
     3001 ctcttccacc agcgcatctt taagctcctt tctctcctcc tgagaaattt ctgtcttcag
     3061 tacaaggttt gcctgcgtct cctccccttc tatatttact ttcaccttat atacttctat
     3121 gcctcttttt tccagagttt cccggatttt ctgttccagt tccagttcgt aacctttttc
     3181 cagatagact ttctgaatat tctgaaattc ccggagttct ttctctctgt tttcttcaga
     3241 aaaattatcc agaaaacttt ccgtcagttc cgaacctttt ccaagaatgg aaaatatcgg
     3301 ggtactcatc ataaaaatca gcagcaggcc cataaaaaag cgtacatatt tctcatactt
     3361 ctggtcaggt atcagattca gaaccgcagt gaaaaagata taaaatactg ccagattccg
     3421 aatccataaa tacagctctt ctttcatatg tttcctccgg caatctttct gtcagttcat
     3481 caggatacag aaactacaac tatcaggaat gtcagcatgc aaagtacttc tgctgtcagc
     3541 aaaagcttta aaagcattgc acatccctcc cccattgtac tcagggctcc cacaactctt
     3601 ttgtcagaaa ttggctgtgc aatcgcagcc agaaaacgat aaactagaga aagacttcca
     3661 taatgaataa tcggtcctgc tccaatcagc aacagaagga tcacgattac tgcgccaaaa
     3721 ctgtttcgca caatcactgc actggtcagc actaattctg ttacagcggt cactgcattt
     3781 ccaatgccgg gaatcgcact cgctgccttg ccaacagcag aacgcttcat ggcatccatc
     3841 accgggctca ccatattccg cacaatctgc agaccaacaa tagctcccag aagagttttc
     3901 aacccccagt ttacagctac atccaaaagt tctgccattt ttgtaagcat ctcctctttt
     3961 gacaaataat taaccatttt cagaagcaaa ctcaggttta cagcaggcag aaacagattt
     4021 gcaagaagcc actgaaccgc ccagatcatc agcaaaattc cgtcataaaa tgcagcagct
     4081 gttgatgatc cggttgatgc agc
//
LOCUS       DBMDXG010000166         4089 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5157_length_4089_cov_1.665747, whole genome shotgun sequence.
ACCESSION   DBMDXG010000166 DBMDXG010000000
VERSION     DBMDXG010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5157_length_4089_cov_1.665747"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="ACZPD3_05675"
     CDS             complement(<1..543)
                     /locus_tag="ACZPD3_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR1415526.1"
                     /translation="MEQKKALNRKKLKVILVICIVMIVTAGIGLAVYIHHVDTVTLGR
                     KVSVYRLDVSKLTVEEAQQKISEAFQNKTITFHEDGKDVYNVSMEQLGYSLDQDILKS
                     ALETLKQERSGTFKLFASQRDYKIDYQIIRDEAQEQAALSADHFDEKDRTEPTDAHIR
                     YSKKKQKYVLVKQVSGNQIDE"
     gene            complement(682..795)
                     /locus_tag="ACZPD3_05680"
     CDS             complement(682..795)
                     /locus_tag="ACZPD3_05680"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415527.1"
                     /translation="MGYDNRIRTKYTAKWGAQIAGMIFQTRSKVTGGCNLE"
     gene            742..882
                     /locus_tag="ACZPD3_05685"
     CDS             742..882
                     /locus_tag="ACZPD3_05685"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415528.1"
                     /translation="MRAPLCGIFCPNSVVVAHQVFESIKKSHITRMRDFLLMRLSSGF
                     VN"
     gene            complement(865..1728)
                     /gene="fba"
                     /locus_tag="ACZPD3_05690"
     CDS             complement(865..1728)
                     /gene="fba"
                     /locus_tag="ACZPD3_05690"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR1415529.1"
                     /translation="MLVNATEMLKKAKAGHYAVGQFNINNLEWTKAILLTAQELNSPV
                     ILGVSEGAGKYMTGYKTVVGMVNGMMEELNITVPVALHLDHGSYEGCLKCVEAGFSSI
                     MFDGSHYPIEENVAKTKELVKIVAEHGMSLEAEVGSIGGEEDGVVGMGECADPQECKM
                     IADLGIDFLAAGIGNIHGKYPANWKGLSFETLDAIQKLTGEMPLVLHGGTGIPADMIK
                     KAIDLGVSKINVNTECQLSFAAATRKYIEEGKDQQGKGYDPRKLLAPGFEAIKATVKE
                     KMELFGSVNKA"
     gene            1899..1980
                     /locus_tag="ACZPD3_05695"
     tRNA            1899..1980
                     /locus_tag="ACZPD3_05695"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1933..1935,aa:Tyr,seq:gta)
     gene            complement(2101..>4089)
                     /locus_tag="ACZPD3_05700"
     CDS             complement(2101..>4089)
                     /locus_tag="ACZPD3_05700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR1415530.1"
                     /translation="EPKAGDTVTIKFRTQRNNVDSVYLVSQEQRVQMEICGTENGFDY
                     YSAQVTIGADIFRYYFEIQYGWVTCYYNNQGVCMKHEGRMDFEIYPGFDTPKWAKGAV
                     MYQIYVDRFLNGDPTNDVVTGEYHYIGDKSVQVEQWNKIPAVMGVREFYGGDLQGIMN
                     KLDYLQDLGVEVIYLNPIFVSPSNHKYDCQDYDYVDPHYGRIVEDCNEGILLGDDDDN
                     SHAWKYIKRVTDKKNLEASNELFAKLTAEIHRRGMKIILDGVFNHCGSFNKWMDRERI
                     YENQEGYPKGAYVSADSPYRNFFSFNDPNAWPYNTSYDGWWAHDTLPKLNYEGSRELY
                     DYILRVGQKWVSAPYNVDGWRLDVAADLGHSNDFNHQFWKDFRKAVKAANPNAIILAE
                     HYGNPEGWLKGDEWDTVMNYDAFMEPLTWFLTGMEKHSDEYREDLLGNSEAFIGAMKT
                     HMRALHMSALQTAMNELSNHDHSRFLTRTNHRVGRISYAGPEAASEGVNPAVMREAVT
                     IQMTWPGAPTVYYGDEAGLCGFTDPDNRRTYPWGREDYQMIDFHRVMIRIHKKYEVLK
                     TGSLGFLWNDYQGLCYARFSHDEQIIVIVNNQEEGREVEIRLEQAGISRLEDTKLKRV
                     VMTSAVGFTEECKEYTAAAGILKITMPAFGAVVLHHKN"
BASE COUNT         1052 a         1013 c          706 g         1318 t
ORIGIN      
        1 ttcatcaatc tgatttccgg aaacctgttt caccaacaca tatttctgtt tctttttact
       61 atatcggata tgtgcatccg taggttctgt tctgtctttt tcatcaaaat gatccgcaga
      121 caatgctgcc tgttcctgcg cttcatctct gatgatctga taatcaatct tataatctct
      181 ctgtgacgca aaaagtttaa atgtcccgct tctctcctgt ttcaatgttt ccagtgcact
      241 tttcagaatg tcctgatcca gactgtatcc cagttgttcc attgaaacat tataaacatc
      301 ctttccatct tcatgaaatg ttattgtttt attctgaaat gcttcagata ttttctgctg
      361 cgcttcttcc acagtaagct tcgatacatc caacctgtac acactcactt tccttcccag
      421 tgtgacagta tccacatgat gtatgtaaac agccagaccg attcccgctg tcacgatcat
      481 cacgatacat attacaagaa tcactttcag cttctttcta tttaaggctt ttttctgttc
      541 catcctttgt tctgctactt cctttcacct tattttagat atctgcaatt attttattat
      601 atagagagat aaaaatcaat ttgtaaaatg cagttcgcct gttgttactg ttcactccgt
      661 tctcagtaac gtagccaaaa ttcattccag attgcaaccg cctgttactt tagagcgtgt
      721 ctgaaaaatc attcctgcaa tctgcgcgcc ccactttgcg gtatattttg tccgaattcg
      781 gttgtcgtag cccatcaggt ttttgagtcc ataaaaaaat cccacatcac aaggatgcgg
      841 gattttttac ttatgaggtt atcatcaggc tttgttaact gatccgaata attccatttt
      901 ttccttaaca gtagctttga ttgcttcgaa gccaggtgca agaagtttac gaggatcgta
      961 tcctttaccc tgctgatctt ttccttcttc gatgtattta cgtgtagctg ctgcgaaaga
     1021 aagctggcat tctgtgttta cattgatctt ggatacgcca aggtcgatag ctttcttgat
     1081 catatctgcc gggatacctg tgccaccgtg aagtaccaga ggcatctctc ctgtcagttt
     1141 ctggattgca tccagagttt caaagcttaa gcctttccag ttagccggat attttccatg
     1201 gatgttaccg atacctgctg caaggaaatc aataccaagg tctgcgatca tcttgcattc
     1261 ctgaggatct gcgcattcgc ccattcctac aacaccgtct tcttcaccac cgatggaacc
     1321 aacttctgct tccagagaca ttccgtgttc agcaacgatc ttaaccagtt ctttagtttt
     1381 tgcaacgttt tcttcgatcg ggtaatgaga tccgtcgaac atgatggatg agaatcctgc
     1441 ttcaacgcat tttaagcatc cttcatagct gccgtggtca aggtgaagag ctacaggaac
     1501 agtgatgttc agttcttcca tcattccgtt aaccatacca acaactgttt tataacctgt
     1561 catatattta ccagcacctt cagatacacc aaggattaca ggagagttca gttcctgagc
     1621 tgttaaaagg atagcctttg tccactcaag gttgttgatg ttgaactgtc caactgcata
     1681 atgacctgct tttgcttttt tcagcatttc tgttgcattt actaacatga taaattcctc
     1741 ctaatctgta gtaactgtgt tcattctcag ttaccctaat caatctccga tatggaatta
     1801 catctatatc cagatatggt tatataataa ttttctgcaa tttataaaaa agtacttgca
     1861 ttatttaaat atttcatata taataagttt cggtttaagg atggattccc gagtggccaa
     1921 agggggcaga ctgtaaatct gttagcaacg ctttcgaagg ttcgaatcct tctccatcca
     1981 tcaaaaggtt cagacatttg tctggatctt ttttttgtta tattataaag cggcagcccc
     2041 ttcttccgca agaggctgcc gtccgggtca ggcggataag atcatccgct tcgcattttg
     2101 ttaattttta tgatgtaaga ccactgcgcc aaatgccggc attgtgatct tcagaattcc
     2161 ggctgctgct gtgtattctt tacattcttc cgtaaatccc actgcagatg tcatgacaac
     2221 acgtttcagc tttgtatctt ccagacggct gattcctgcc tgttctaacc gtatctccac
     2281 ttccctgcct tcttcctgat tatttacgat cacaattatc tgttcatcat gagaaaatct
     2341 cgcataacaa agtccctgat aatcattcca gagaaatccc agtgatcccg tttttaacac
     2401 ttcatatttc ttatggatac ggatcattac cctgtggaag tcgatcatct gataatcctc
     2461 acgtccccag ggataagtac gtctgttatc cggatctgtg aatccacaga gtcctgcctc
     2521 atctccgtaa tatacagtcg gtgcgcccgg ccaggtcatc tggatggtaa ccgcctcacg
     2581 cataactgcc ggatttactc cctcagatgc cgcctccgga cctgcatagg aaattcgtcc
     2641 taccctgtga ttggttctgg tcaggaatct ggaatggtca tgatttgaca attcattcat
     2701 ggctgtctga agtgcagaca tatgaagcgc tctcatgtgc gttttcatag ctccgataaa
     2761 agcctcactg tttcctaaca ggtcttcacg atactcgtca ctgtgcttct ccatacctgt
     2821 caggaaccag gtcagcggct ccataaatgc gtcatagttc atgactgtat cccattcatc
     2881 acctttcagc cagccttccg gattaccgta atgctctgcc aggataatgg catttggatt
     2941 ggcagctttc actgccttcc ggaaatcttt ccagaactga tggttaaaat cattgctgtg
     3001 tcccagatcc gccgccacat ccagtctcca accgtctaca ttataaggag cagataccca
     3061 tttctgtcca acacgaagaa tataatcata aagttctctg gaaccctcat aatttaattt
     3121 aggcagtgta tcgtgtgccc accatccatc ataggaggtg ttatatggcc atgcattagg
     3181 atcattaaag gaaaagaaat tacgataggg actgtccgca gaaacataag ctcctttcgg
     3241 atatccttcc tgattctcat aaatacgttc tctgtccatc cacttattaa aagaaccgca
     3301 gtgattgaac acaccatcca gaatgatctt cattcctctt ctgtggatct ctgctgtcag
     3361 tttcgcaaaa agctcattac ttgcttccag attcttctta tctgtcacac gtttgatata
     3421 tttccatgca tgggaattat catcgtcatc tccaagaagg attccctcat tacaatcttc
     3481 tacaatcctg ccgtaatgtg gatccacata atcataatcc tgacagtcat acttatgatt
     3541 acttggagaa acaaaaatcg gatttagata aataacctcg actcccagat cctgcagata
     3601 atccagtttg ttcataattc cctgcaggtc accgccgtaa aattcacgca cacccatcac
     3661 tgccggaatc ttattccact gttctacctg tacactctta tctccaatat aatgatattc
     3721 tccggtcaca acatcattgg taggatctcc gttaagaaaa cggtctacat aaatctggta
     3781 cattaccgca cctttggccc actttggagt atcaaagccc ggataaatct caaaatccat
     3841 cctgccctca tgtttcatac agactccctg attattgtaa taacaggtta cccatccata
     3901 ctgtatctca aaataataac gaaagatatc cgctccaatc gtcacctggg cagaataata
     3961 atcaaatccg ttctctgtcc cgcaaatctc catctgcaca cgctgctcct ggcttaccag
     4021 atatacagaa tctacattat ttctctgggt acggaatttg atcgttactg tatctcctgc
     4081 ctttggttc
//
LOCUS       DBMDXG010000167         4087 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5159_length_4087_cov_1.773457, whole genome shotgun sequence.
ACCESSION   DBMDXG010000167 DBMDXG010000000
VERSION     DBMDXG010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5159_length_4087_cov_1.773457"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="ACZPD3_05705"
     CDS             <1..575
                     /locus_tag="ACZPD3_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1415531.1"
                     /translation="LAANPTKKIDFNLYCDKVLTADILNGLKEKKYDLGFCSMIDNEP
                     LIEFIPVARQELVVIVPSDHPLAAKEEVCLEDTIPYKQIIFKKRSGLRHIIDGLFERI
                     GQKPDVAYEIDEDQVAAGFVSNGFGICVAPNIPILQSLNVKILPLVSPSWQRNFYLAM
                     LKNVYHPPVVEAFKKYVIEQAQKEWDYKTS"
     gene            complement(727..1515)
                     /locus_tag="ACZPD3_05710"
     CDS             complement(727..1515)
                     /locus_tag="ACZPD3_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR1415532.1"
                     /translation="MNQLEILRESLGQCDEIILDALIMRNRIVEDIMAYKEANGLQIL
                     QPEQEAKQKEWLEKRMEGRRHKDEVSDVFECIRTNSKRIQARKLFNYNIVLIGFMGAG
                     KSTISDFLKNVFAMDVVEMDQIIAQRQGMSISDIFETYGEQYFRDLETNLLIEMQSRS
                     NVVISCGGGTPMRECNVVEMKKNGRVVLLTAKPETILDRVKNNHDRPLIENNKTVPFI
                     ADLMEKRRAKYEAAADIIIETDGKNELEICEELVHRLRTMDEEK"
     gene            complement(1771..3003)
                     /locus_tag="ACZPD3_05715"
     CDS             complement(1771..3003)
                     /locus_tag="ACZPD3_05715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1415533.1"
                     /translation="MKHIAAVIVVTAVLLFTQTYTSARGAEYKIPQTVDMTPVAEEPA
                     ELYALSAVLMDGESGRVLYEKDGERPLANASTTKVLTCIVALENSSGDDYVQVSQNAA
                     SQPEVKLGLQKGEQYYLEDLLYSLMLKSHNDTAVAIAEHCGGSVEGFARMLNRKAKQI
                     GCKDTYFITPNGLDAEDENGKHHTTAKDLALIMRYAVKNETFLHIAQTRDYTFSEITG
                     KRTFSVHNANAFLDMRDGVLAGKTGYTSQAGYCYVCAWEKEGKTFIVSLLGSGWPNHK
                     TYKWSDTEKLLDFGDYNYEYETYWKEPQTGKILVTDGVEDGQDIGTKIYLRGKCSVTA
                     YDREKEVLLKKGETVTCKIEIPQKVSAPVLKGEKLGRIAYYLDGKLIDFYPVYAEKSV
                     EKISFKWYTEKVFHDFFH"
     gene            complement(3184..3978)
                     /locus_tag="ACZPD3_05720"
     CDS             complement(3184..3978)
                     /locus_tag="ACZPD3_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1415534.1"
                     /translation="MPTTYAHDLFGKEVYKRLPSDMKALIRRHGDLYRIGLHGPDILF
                     YYMVSKNPVTQFGIEMHHEKARAFFEEGMRQVRRNDDEALFAYLLGFGCHYILDSTCH
                     PYVNQMAAEGVIPHIVLEKEFDRVLMEETGKDPDHYYPACGIMPKMEYARVIHRAIPL
                     VKTINIYISVRMMKILTNFMVCDDHGRKRRILGKLLRLGGESIGSVIEHFMTAEAVEQ
                     AKAPMPELERLYREAVPEAVEYLGELYTLREGAYHLSKRWDRTYNG"
     gene            complement(3997..>4087)
                     /locus_tag="ACZPD3_05725"
     CDS             complement(3997..>4087)
                     /locus_tag="ACZPD3_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR1415535.1"
                     /translation="LESLPVPDPVQIEDFKAEAEEEIQLKLDV"
BASE COUNT         1024 a          975 c          812 g         1276 t
ORIGIN      
        1 ttctcgcggc aaatccaacg aaaaagattg attttaatct gtattgtgac aaagttttaa
       61 cagctgatat tttaaatggt ttaaaagaga aaaaatatga tctgggtttc tgctctatga
      121 ttgataatga accactgatt gaatttatac ctgttgccag acaggaactg gtggtgatcg
      181 tgccttctga ccatccgctg gcagcaaaag aggaggtttg tctggaggat actattcctt
      241 ataaacagat catatttaag aaaagaagtg gtctgagaca tattattgac ggattgtttg
      301 agcgcatcgg tcagaaaccg gatgttgcct atgagattga tgaagatcag gttgctgcag
      361 gctttgtctc caacggcttt ggaatctgcg tagctcccaa tattccaatc ctgcagtctc
      421 tgaatgtaaa gatccttccc ttagtttctc caagctggca gagaaacttc tatctggcga
      481 tgctgaagaa cgtataccat ccaccggttg tggaagcatt taagaaatat gtgatcgaac
      541 aggcacagaa ggaatgggat tataagacca gctgatctga cacaataaaa tatcagaata
      601 caaaaagata tgggactgtc ccgtcagtcg agatacccca tatcttttta taataaccga
      661 tacattcaat attatgactt gatatccggc gcttctgata tcataaacca gatctgtcat
      721 gttatattat ttttcttcat ccatggtgcg gagtctgtgt accagttctt cgcagatttc
      781 cagttcgttc ttgccgtctg tttcgatgat gatatcagcc gctgcctcgt atttagcgcg
      841 gcgtttctcc atcagatctg cgataaatgg aacagtctta ttgttctcga tcagcggacg
      901 gtcatggtta tttttgacgc ggtcaaggat tgtctccggt tttgctgtga ggagaacaac
      961 acggccattc ttcttcatct ctactacatt gcactctctc attggagtac caccgccgca
     1021 ggagatcaca acattgctgc gtgactgcat ttcgataagc aggtttgtct caaggtcacg
     1081 gaaatactgc tcaccgtatg tctcgaagat atcggaaata ctcattccct gtctctgggc
     1141 aatgatctga tccatctcta ccacatccat ggcaaataca ttcttaagga aatcagaaat
     1201 tgtggacttt ccggcaccca tgaaaccgat cagcacgatg ttgtagttga agagttttct
     1261 tgcctggatc cgtttactgt ttgtgcggat gcattcaaat acatcggaaa cctcatcctt
     1321 atggcgtctg ccttccattc tcttctccag ccattctttc tgcttcgctt cctgctcagg
     1381 ctgtaaaatc tggagtccgt tggcttcttt atatgccata atgtcctcca cgatcctgtt
     1441 acgcatgata agtgcatcca gaatgatctc atcacactgg ccaagactct ctctgagtat
     1501 ttctaactga ttcatatctc tttcctctgt ctttcctatg tcagcttgca gcagaccgca
     1561 gatcagccac aggctgacat aaactattca cgaacggcaa tatatgaatg ccgactgtta
     1621 agatttatta tatcaatatc gctttcgtat agcaaccctt taaaccatta aattgtttca
     1681 tctgttcgat agtttttttc tatgaattcg acagacgatt ctatggcaag actctgatga
     1741 cgtttcttta aggttcccat ataaatgtat ttaatgaaaa aaatcatgaa atactttttc
     1801 agtgtaccat ttaaaactga ttttttccac tgatttctcc gcatagaccg gataaaaatc
     1861 tatcagtttt ccatccaggt aataggcaat gcggccaagc ttctccccct taagaaccgg
     1921 tgcactgact ttctgtggaa tttctatttt acaggttaca gtttcccctt ttttcagaag
     1981 gacttctttt tccctgtcat atgcggttac actgcattta cccctcagat agattttcgt
     2041 tcctatatcc tgaccatctt ccacaccgtc tgttaccagg atttttccgg tctgtggttc
     2101 tttccagtag gtttcgtact catagttgta atctccgaag tccagcagtt tctctgtgtc
     2161 actccattta taggttttgt ggttgggcca cccactgcca aggaggctga cgataaatgt
     2221 tttgccctct ttttcccagg cacatacata gcagtagcca gcctgggaag tatagcctgt
     2281 ttttccggca agaactccgt ctctcatgtc gagaaatgca ttggcattat gtacggaaaa
     2341 ggttctcttt cctgtgatct cagagaatgt atagtctctg gtctgtgcaa tgtgcaggaa
     2401 tgtttcgttt ttaacagcat atcgcatgat cagtgccaga tctttggctg ttgtgtgatg
     2461 ttttccattc tcatcctcag catccaggcc attgggtgtg ataaaatacg tatccttaca
     2521 tcctatctgt tttgctttcc ggttgagcat tctggcaaag ccttccacgc ttcccccgca
     2581 gtgttctgca atggcaaccg ctgtatcatt gtggcttttc agcataaggg aatacagaag
     2641 atcctccaga taatactgtt ctcctttctg aagtcccagc ttcacttccg gctgggatgc
     2701 cgcattctga gaaacctgaa cataatcatc ccccgaagag ttctccagtg ccacaataca
     2761 ggtaagcacc ttggttgtgc tggcatttgc cagaggacgc tccccgtcct tttcatagag
     2821 tactctgccg gactctccgt ccatgaggac tgctgataat gcgtacagtt ctgccggttc
     2881 ttctgcaaca ggtgtcatgt ctactgtctg aggtattttg tactccgctc cccgggcgga
     2941 ggtgtatgtc tgcgtaaaca ataataccgc cgtcacaaca atgacagcgg caatatgttt
     3001 cattctcata ctccacctca catgctggca atccatgaat atcactgttc ataatttacg
     3061 gttattcaca gatttgttgt ttacaggata tctgcaggat tttgcttcac cctataagat
     3121 aatttatgcc tgcatgtccg gaatatgact gtcacagttc cgaaaaatat cgggatatgc
     3181 ccattacccg ttataagtcc ggtcccatct tttgctcaga tgataagctc cctctcttaa
     3241 ggtataaagt tctcccagat attccacagc ttcaggaacc gcctctctgt aaagcctctc
     3301 aagttcaggc atgggagctt ttgcctgctc tactgcttcg gcagtcatga aatgttcaat
     3361 gacagagccg atggactctc ctcccagtct cagaagtttc cccagaatcc tgcgttttct
     3421 tccatggtca tcacacacca tgaaattggt aagaattttc atcatcctga cagaaatata
     3481 aatgtttatg gttttcacca gaggaatggc cctgtggatc actctggcat attccatctt
     3541 cggcatgatc ccgcaggccg gataataatg gtcgggatct ttacctgtct cttccataag
     3601 cacacggtca aattccttct ccagaacaat gtgaggaatg actccttctg cagccatctg
     3661 atttacatag ggatggcatg tggagtccag aatataatga cagccaaacc caagaagata
     3721 tgcaaaaagt gcctcatcat catttcttct gacctgccgc atcccttctt caaaaaaggc
     3781 tcttgccttc tcatgatgca tctcaatccc gaactgggtg acaggattct ttgaaaccat
     3841 atagtagaac aggatatccg gcccatgtaa accgatcctg tacaagtccc catgtctgcg
     3901 gatcagggct ttcatatctg aaggcagtct cttatatact tcttttccaa ataaatcatg
     3961 tgcatatgtg gttggcatta ttttttctcc tttatcttac acatccagtt tcagctggat
     4021 ttcttcctct gcctctgcct taaagtcctc tatctggaca gggtcaggga caggcaggga
     4081 ttccaga
//
LOCUS       DBMDXG010000168         4063 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5190_length_4063_cov_2.619889, whole genome shotgun sequence.
ACCESSION   DBMDXG010000168 DBMDXG010000000
VERSION     DBMDXG010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5190_length_4063_cov_2.619889"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(133..1875)
                     /locus_tag="ACZPD3_05730"
     CDS             complement(133..1875)
                     /locus_tag="ACZPD3_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR1415536.1"
                     /translation="MKLSDYIVTFLQKKGIRHFFGYQGTMIAHLVDSIERNPETENHS
                     GYNEQGAAFAACGYAQAKEECACAYATSGPGAINLLSGVADAYYDSLPVIFLTGQLNT
                     YEYSGIKGLRQQGFQETDIVAMAKPITKYAVQIRNPEDIVEELNKAYHIATTGRRGPV
                     LIDLPMNIQRSEVENPVYDMTFEDKHTDAAAAQQAADTILEALEQAKRPVIMLGHGVD
                     SSFSQQKLIRFAQKRQIPIITSVLAKSVLGYDHPLNFGCIGGAYGHRYANMIANAKSD
                     LLLCFGISLCTRQIGTKVHEFARGAKIIRIDIDPYNLQRDIHENGINEVKLQAETGAV
                     IDCLAKAAAPDTEGISDWLNVCTEIKENLQAVDDATPERYPNRMIADLSDMLEDTSAI
                     AVDVGQHMVWSYQSFKNHEGQKLLFSGGHGAMGYGLPAAIGAYYATGKPTACICGDGA
                     LQMNIQELEWVKRENLPVKIMVMNNEALGMIRHLQRDYFDCLFAGTTDGCGFASCNFA
                     EVAKAYGIPALRMLCDAVREEAPEFLEGEGPKLLEVMLEHGTFAYPKTCLGEPIHNQQ
                     PYAPKEVYDRLMEL"
     gene            complement(1887..3047)
                     /locus_tag="ACZPD3_05735"
     CDS             complement(1887..3047)
                     /locus_tag="ACZPD3_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1415537.1"
                     /translation="MNKKQKVILSLLQEIDEICRRNKIEYYLSPRLTLCAVEGHPFPQ
                     NPMFGVVLMKTADMERFRLAVDEDPREKRALESMKSHKWFSGFYLRYTNTDTLCLNLD
                     NTRDYAFPGIGVNIFPLRTPAASVKAERRLSRDENAWTELCHINYADRNFRSRVNRTI
                     MRLQCMITGRQGQAAHLYDRLVRSCQQPGANKYILKRRKQTTVFPAEIFAQSKRVTLE
                     VAELQVPAKTAEYLTISYGKNYKDAKEPRYVTPIALVVSARVSYTQFWKESGNFEKYC
                     KERMKNARKLARSRRHKDYFNECWDYVEFCGERMNLSVSYEKQKDYIKNLYKNEDYMT
                     LERVFRPYFKMMQKSLQKNELFAEDEEIFDIYVDVLEKTGKTVQRSKIGTLI"
     gene            complement(3072..>4063)
                     /locus_tag="ACZPD3_05740"
     CDS             complement(3072..>4063)
                     /locus_tag="ACZPD3_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1415538.1"
                     /translation="LHLAIHPEDGYFLKYKADILWMDGEMQKAAELYLQVKNKTTNGI
                     VWMEMDRFFRGYKSEILKSCEELIANHNKKEALALMELWSRLIPEDDDIQGALYLAKT
                     VCARTQSEIEKEIGEIRAVIGTQMITPVSVEKNPGKSRKQIKSDRTSDETSADDVNKK
                     VSDPSAETEEVKAPADIQVKVSEEHKMYRKALTRAWKRLGYSDELAELRTQIICTGEE
                     SELEWLAEQVRNRQFRREEKACAYKLVGDVRMKQGQTREAFANYRKALEYEMPSYVRT
                     ELYRIFINDLNDGSRQAKSFGKKTDITVVLDKWLDKYESIEDIKKIVQTVTVK"
BASE COUNT          922 a         1067 c          793 g         1281 t
ORIGIN      
        1 tattcatcta taatacagac agatctgttc tctgtcatgc gggagtctcc ccacttccat
       61 aaaacggact gcctgcaaaa aaattccttc tataattata tattccacat taccgaaata
      121 tattcttcac tcctacaatt ccatcagtct gtcataaact tccttcggtg catatggctg
      181 ctgattatgg atcggttcac ccagacaggt cttgggatat gcaaaagttc catgctccag
      241 cataacttcc agaagtttcg gaccttcccc ttccagaaat tccggtgctt cttctctgac
      301 tgcatcgcag agcattctca gtgcgggaat gccgtaagcc tttgccacct ctgcgaaatt
      361 acaggaagca aaaccgcagc cgtctgtggt tcctgcgaac agacagtcaa aataatctct
      421 ctgcaggtga cggatcatgc caagtgcttc attattcata accatgatct taacaggaag
      481 attctctctc ttcacccact ccagttcctg gatattcatt tggagtgcac cgtctccgca
      541 gatgcaggcg gtcggttttc cggttgcata atacgctccg atagctgccg gaagcccgta
      601 gcccatagct ccatgtccgc cggagaagag aagtttctgt ccttcatgat tcttgaatga
      661 ctggtaactc cacaccatat gctgtcctac atccactgcg atcgcggagg tatcctccag
      721 catatcactg aggtctgcga tcatacggtt cggatatctt tccggcgtgg catcatcaac
      781 tgcctgaaga ttttccttaa tctctgtgca gacattcagc cagtcactga tcccctctgt
      841 atccggagct gctgcttttg caagacaatc gattactgca cctgtctctg cctgcagctt
      901 cacttcattg ataccattct catggatatc tctctgaaga ttgtatggat caatatctat
      961 acgtatgatc tttgcacctc tggcaaattc atgtactttg gttccgatct gcctggtaca
     1021 cagagaaatg ccaaagcaga gaagcagatc gctttttgca ttggcgatca tatttgcata
     1081 acggtgtcca taggcaccgc cgatgcaccc gaaattcagc ggatggtcgt atcccagaac
     1141 tgatttcgcc agaacgctgg tgatgatcgg aatctgtctc ttctgtgcaa agcggatcag
     1201 tttctgctgg ctgaagctgc tgtccacgcc atggcccagc atgatcaccg gacgttttgc
     1261 ctgttccagt gcttccagaa tcgtatctgc cgcctgctgt gctgcagctg catctgtatg
     1321 tttgtcctca aaagtcatgt catagaccgg attctccact tcacttctct ggatattcat
     1381 gggaagatcg atgagcaccg gtcctctgcg tcctgtggtt gcaatatggt atgctttatt
     1441 taattcctcc acaatgtcct cagggtttcg tatctgaaca gcatatttgg taatcggttt
     1501 cgccattgcc acaatatctg tctcctggaa tccctgctga cgcagcccct taatccctga
     1561 atactcataa gtgttcagct gtcctgtgag aaagatcaca ggcagggaat cataataggc
     1621 atctgccaca ccactcagaa ggttgatggc tcccggtccg ctggtagcat aggcgcaggc
     1681 acattcttcc ttcgcctgtg cgtatccgca ggctgcgaaa gctgccccct gttcattgta
     1741 accggaatga ttctctgtct ccggatttct ctcaatggag tctaccagat gtgcgatcat
     1801 ggttccctga tagccgaaga aatggcggat tccttttttc tgcaaaaacg taacaatata
     1861 atctgaaagt ttcatgttcc ctcccgtcag atcagcgtgc cgatcttact tctctgtact
     1921 gtctttcctg ttttctccag tacgtctaca taaatgtcaa aaatctcttc gtcctctgca
     1981 aagagttcat tcttctgcag actcttctgc atcatcttga aatagggacg aaataccctc
     2041 tcaagtgtca tataatcttc attcttatac aagttcttaa tgtagtcttt ctgcttctca
     2101 taggaaacac tcagattcat gcgctcccca cagaactcta cataatccca gcattcattg
     2161 aagtagtctt tgtgccgcct gcttctggca agtttgcggg catttttcat tcgctcttta
     2221 cagtatttct caaagtttcc ggattctttc cagaactgtg tatagcttac tctcgcactg
     2281 accaccaacg caatgggtgt cacatagcgg ggttcctttg catctttata attctttcca
     2341 tagcttatgg taagatactc tgcggttttt gccggaacct ggagttctgc aacttctaga
     2401 gtgacacgtt tactctgcgc aaaaatttct gcagggaaaa cggtagtctg ttttcttctt
     2461 ttgaggatat atttatttgc tccgggctgc tggcaggatc tgacaagtct gtcatacaaa
     2521 tgtgcagcct gtccctggcg cccggttatc atgcactgca gccgcataat tgtacggttc
     2581 accctgctcc tgaaatttct atctgcatag ttgatatgac agagctctgt ccatgcattc
     2641 tcatctctgg ataatctgcg ttctgctttt acagaagctg ccggcgttct gagcggaaaa
     2701 atattgactc ctattcccgg gaatgcgtag tctctggtgt tgtccagatt caggcacaga
     2761 gtatctgtat tggtgtatct cagataaaat cctgagaacc atttgtggct cttcatggat
     2821 tccagcgctc tcttttccct tggatcttca tccacagcca gacggaaccg ttccatatcc
     2881 gctgttttca tcagcacaac tccaaacatg ggattctgtg ggaaaggatg cccttccact
     2941 gcgcagagtg tcagtcgtgg agagaggtaa tattctattt tattccggcg gcatatttca
     3001 tcgatttcct gtaataatga taaaatcacc ttttgttttt tgttcaactc tttactccat
     3061 ttctattgat gtcacttaac agttacagtc tgcacgatct tcttaatatc ttcaatactt
     3121 tcatatttat caagccattt atcaagcaca actgtaatat ccgtcttctt tccaaagctt
     3181 ttcgcctgtc tgctgccatc gttcagatca ttgataaaaa tacggtacag ctctgttctt
     3241 acataagaag gcatctcata ttccagtgct ttcctataat ttgcaaaagc ctccctggtc
     3301 tgtccctgtt tcatcctgac atcacccacc agcttataag cgcaggcttt ctcttctctg
     3361 cggaactgac ggtttcgtac ctgttctgca agccactcca gttcgctctc ctcacctgta
     3421 cagatgatct gggtgcgcag ttctgcaagc tcatcggaat accccagccg cttccatgct
     3481 cttgtaagag ctttcctgta catcttatgt tcttctgaaa ctttaacctg tatatccgcc
     3541 ggcgctttaa cttcttcagt ttctgctgac ggatctgata cttttttatt cacatcatca
     3601 gccgatgttt catctgatgt tctatctgat tttatctgtt ttctgctttt tcccggattt
     3661 ttctccacac tgactggcgt gatcatctgt gtgccgatca cagcccggat ttcaccgatt
     3721 tctttctcta tctcgctctg cgtacgtgca cacactgttt ttgccagata taatgctccc
     3781 tggatatcat catcctcagg tatcagacga ctccacagct ccatcaatgc gagagcctcc
     3841 ttcttgttgt ggtttgcaat aagctcttca cagcttttca ggatttcaga tttgtatcct
     3901 ctgaagaatc tgtccatctc catccataca attccgttag ttgtcttatt ctttacctga
     3961 agataaagct ctgccgcttt ctgcatctcg ccatccatcc agaggatatc tgccttatat
     4021 ttcaggaaat aaccatcctc aggatggatg gcgagatgca gaa
//
LOCUS       DBMDXG010000169         4061 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5192_length_4061_cov_2.033838, whole genome shotgun sequence.
ACCESSION   DBMDXG010000169 DBMDXG010000000
VERSION     DBMDXG010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5192_length_4061_cov_2.033838"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="ACZPD3_05745"
     CDS             <1..207
                     /locus_tag="ACZPD3_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR1415539.1"
                     /translation="ADKAELFQIAETFKSKVIDYGKDSLLLEFVQTAIKNDAVVKLIK
                     EEFHRIEVVRGGSVGIESISMMER"
     gene            582..1220
                     /locus_tag="ACZPD3_05750"
     CDS             582..1220
                     /locus_tag="ACZPD3_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1415540.1"
                     /translation="MNTTTTTTTTAAAKRSSTLYMVELAMMIAIILLMSFTPLGYLRT
                     PGLSITLLTIPVAVGAIILGPKGGAVCGLAFGATSFYMAVTGSSAFAAALFNINPFGT
                     FIVCIVARVLEGWITGLIFAALYKSPAKKFSYYVASLACPLLNTFFFMGFLCLFFYNT
                     DYIQGIVSSLGVSNPVVFVAAFVGVQGLIEAGFCFVVGSIVSKALSVALKRA"
     gene            1479..2444
                     /locus_tag="ACZPD3_05755"
     CDS             1479..2444
                     /locus_tag="ACZPD3_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR1415541.1"
                     /translation="MSLLKEISITELSNLRIGHTSDHDAKTGVTVLYFPNGAKAGCDI
                     SGGGPASRETPLTSPVTADNPINAIVLSGGSAYGLAAADGVMNYLESQNIGYNTGAAL
                     VPLVCQSCIYDLSYGDPKIRPTAKMGEEACRQAFAGTDARTGNIGAGTGATVGKLYGM
                     KQSMKSGLGIAAVSVGNFQMAAIVVVNALGDIFSPQNGQKIAGLKTPDRSGFLDSVHE
                     LYRFMTPHDQFTGNTTIGAVITNGAFSKAELNKIASMTRCAYARCINPVATMADGDSI
                     YAASIGDVSVDINMAGTLAAEVMAQAIQNAIHTSRIQDEEFLKYV"
     gene            complement(2516..2758)
                     /locus_tag="ACZPD3_05760"
     CDS             complement(2516..2758)
                     /locus_tag="ACZPD3_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415542.1"
                     /translation="MERLIFHIDVNSAFLSWESARRVSQGLPEKMWPLPVRNLLFLGK
                     ASEQKLLNQGIRTIGDLAREREANVQLWLGEKPEHR"
     gene            complement(2774..3958)
                     /locus_tag="ACZPD3_05765"
     CDS             complement(2774..3958)
                     /locus_tag="ACZPD3_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1415543.1"
                     /translation="MKTNYNRTVQACFIGYVVQAIVNNFVPLLFLTFQGSYGISLQKI
                     TLLVTFNFGIQLLVDLASIGFVDRIGYRASMILAHAMAAAGLILLTVLPECLGDPFVG
                     LLIAVMIYAIGGGLLEVLVSPVVEACPTDNKEKAMSLLHSFYCWGHVGVVLLSTLFFR
                     IFGIANWKYMALVWALIPIANGIFFTRVPIAPLLDEGEKGLTMGELFKKKIFWVLMLM
                     MLCAGASEQAVSQWASTLAEKSFGINKTIGDLAGPMAFAILMGSSRAFYGKFGDKINL
                     ERFMQYSAVLCMVSYLMIAFIPVPALGILGCAVCGLSVGIMWPGTFSMAAASVKRGGT
                     ALFALLALAGDVGCSSGPTYVGMISGALNDNLKLGIFAALIFPVLMIMGIKMIGSLQK
                     YD"
BASE COUNT         1148 a          974 c          809 g         1130 t
ORIGIN      
        1 gcagataaag cagaactgtt tcagattgca gaaaccttca agtcaaaagt cattgactac
       61 ggcaaagaca gcctgcttct ggaatttgtc cagacagcca tcaagaatga tgcagttgtc
      121 aaactgatca aggaagaatt ccacagaatc gaagtagtac gtggtggaag tgtgggaatt
      181 gaatccatca gcatgatgga acgctaaatc tcataatcaa tattttttgc agaatataga
      241 aacgtctcta tatcctgtat ttattttcaa acatactata aaatattgca gtttttaatc
      301 aaatgtgtta ttgtttacgg gagcgttatt cgaaagaaaa tgcatctgat ttccacatct
      361 gacagtctgg tggataaagt catccacttc acagtctggt taaatttgtg aggcgacctg
      421 taaggcgtct acacaccgga aacttttacc ggaagatatt tcttgtttca tatcttttgt
      481 tctgatgtat atttttcata acgcctgatc cccatactct aaactgtctg aaaagcaggg
      541 gacgcattgc gccgttttat cagaccaaaa ggagaattca tatgaacaca acaactacaa
      601 ctactacaac agctgccgca aaaagaagca gcactcttta catggtagag ctggctatga
      661 tgattgccat tattctgctc atgtccttta ccccgctggg ctaccttcgt actcccggct
      721 tatccatcac tcttctcacc atcccggtag ctgtaggagc tatcatcctc ggaccgaaag
      781 gtggtgctgt atgcggactt gctttcggtg caaccagctt ctatatggct gttacaggaa
      841 gttcagcttt cgctgctgca ctttttaaca ttaatccatt tggcactttt attgtatgta
      901 tcgtggcacg tgtgctggaa ggatggatca caggacttat tttcgcagca ttatataaat
      961 caccggcgaa gaaattttcc tattacgttg cttcactggc atgcccgtta ctgaatactt
     1021 tcttcttcat gggcttttta tgtctcttct tctacaacac agattacatt cagggtattg
     1081 tttccagtct tggagtaagc aatcctgttg tcttcgttgc tgcttttgtg ggcgttcagg
     1141 gactgatcga agcgggcttc tgctttgtag tgggaagtat cgtttccaag gcactttctg
     1201 tggcactgaa acgtgcataa tctttaaaag aacagccttt tccaaaaaac ctgactacat
     1261 tgtcaccgta gtcaggtttt tttatctggc acatttaaaa cagaaatcct acctgattca
     1321 attaatatct atagcaatct cacaaaataa tgcaatcaag acagttcagc agagtgcgaa
     1381 agacaaggaa aatatctgac gcagtacttc acattctgat gggctggatt gctctcagta
     1441 tttcatagaa ttgctatcac ataataaagg agatttttat gtcattatta aaagagattt
     1501 ctattacaga gctttcaaat ttacgcatcg gtcatacttc tgaccatgat gcgaagaccg
     1561 gtgtaactgt tctttatttt ccaaatggag caaaagcagg ctgcgatata agcggaggcg
     1621 gccctgcttc cagagaaact cctctcacct ctcctgtcac tgccgacaat cccatcaatg
     1681 ctattgtctt gtccggaggt tctgcttatg gactggctgc tgctgacggt gtgatgaact
     1741 atctggaatc acaaaatatc ggttataaca ccggtgcagc cctggttcct cttgtatgcc
     1801 agtcctgtat ctatgacctc tcttacggag atcctaaaat acgtccaact gcaaaaatgg
     1861 gcgaagaagc ctgccgtcag gcatttgcag gaacagatgc cagaaccggg aatatcggcg
     1921 caggtaccgg tgcaactgtg ggaaaacttt acggtatgaa acaatccatg aagtcaggtc
     1981 ttggtattgc cgctgtatca gtgggaaatt tccagatggc cgcaattgtc gttgtcaatg
     2041 cactgggaga tatctttagt ccacaaaatg gtcagaaaat cgcagggctg aaaacgcctg
     2101 acagaagcgg gtttctggat agtgtccacg aattataccg ctttatgaca cctcatgacc
     2161 agtttaccgg caataccacc atcggggctg tgatcaccaa tggggcattt tcaaaggctg
     2221 aactgaataa aattgcctct atgacccgct gtgcctatgc cagatgcatc aatcctgtag
     2281 caactatggc tgacggcgat tccatctatg ctgccagcat tggcgatgtt tctgtagata
     2341 tcaatatggc aggaactctc gccgcagaag tcatggctca ggctatccaa aatgccatcc
     2401 atacttcccg gatccaggat gaagaatttt tgaaatatgt ataacagtcc gctataaaag
     2461 cgcagagaaa aagtatcatc tctgcgcttt tattcttcct gtctgtcttc atttttcatc
     2521 tgtgctctgg tttttctcca agccagagct gtacatttgc ttcccgttcc cttgccagat
     2581 caccaatagt acggattccc tgattcagaa gcttctgctc ggaggctttt cccagaaata
     2641 acaggttacg gacaggaagt ggccacattt tctccggcag cccctggctt acccttctgg
     2701 ctgactccca ggaaagaaat gcgctgttta catcaatatg aaaaattaat cgttccatac
     2761 tttttaatat atctcaatca tatttttgca gacttccaat catcttaatc cccattatca
     2821 tcaacacagg gaaaatcagt gccgcaaaga ttccaagttt cagattatca ttcagtgctc
     2881 ctgaaatcat ccccacatag gttggtccgg atgagcatcc cacatctcca gcaagggcaa
     2941 ggagggcaaa cagggctgtt ccgccacgct ttactgaggc agccgccatg ctgaaggttc
     3001 ccggccacat aatacctaca gacagaccgc aaaccgcaca tccaaggatt cccaacgcag
     3061 gtaccgggat aaatgcgatc atcagatagg aaaccatgca cagaactgca ctgtattgca
     3121 taaagcgttc cagattgatt ttatctccaa attttccata aaatgccctt gaacttccca
     3181 taagaattgc aaacgccatg ggacctgcca gatctccgat agttttattg attccaaagc
     3241 ttttctcagc cagagtagaa gcccactggc ttaccgcctg ctcactggct cctgcacaga
     3301 gcatcattaa catcagaacc cagaagattt ttttcttaaa aagctctccc atagtcagcc
     3361 ccttttcacc ctcatccagc agtggagcaa tgggaactct ggtaaaaaag attccatttg
     3421 caatgggaat caatgcccac accagcgcca tatatttcca gtttgcaatc ccgaaaatac
     3481 gaaagaataa tgtagaaaga agtaccactc ccacatgtcc ccaacagtaa aaggaatgaa
     3541 ggagactcat tgctttttcc ttattatctg tagggcaggc ttccaccaca ggacttacca
     3601 gcacctccag caatccgcct ccgatggcat agatcataac tgcaatgaga agtcccacaa
     3661 acggatctcc caggcactcc ggcaatactg tgagcaaaat cagacctgca gccgccattg
     3721 catgtgccag gatcatagat gcacgataac ctattctgtc cacaaatcca atcgatgcca
     3781 gatctaccag caactggatt ccgaaattaa aagtaaccag aagtgtgatt ttctgcagcg
     3841 aaatcccgta actgccctga aatgtcagaa acagcagagg aacgaaattg ttcacaatgg
     3901 cctgcactac atatccgata aaacaggcct gcactgttct gttgtaattt gttttcatcc
     3961 gatgttatat cctccaatct gaaatttatt aagctgacat cattcagaca ttgatccacc
     4021 aaacttctat atctccacag ttctaataga tttctatccg t
//
LOCUS       DBMDXG010000170         4050 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5211_length_4050_cov_1.650038, whole genome shotgun sequence.
ACCESSION   DBMDXG010000170 DBMDXG010000000
VERSION     DBMDXG010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_4050_cov_1.650038"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="ACZPD3_05770"
     CDS             complement(<1..110)
                     /locus_tag="ACZPD3_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR1415544.1"
                     /translation="MRYPDDIIEEVRMKNDIVDVVSQYVKLNKRGSTYFGL"
     gene            complement(134..1141)
                     /locus_tag="ACZPD3_05775"
     CDS             complement(134..1141)
                     /locus_tag="ACZPD3_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR1415545.1"
                     /translation="MNIRESMEQREQKMLSPYAALSLCSRGRERQEEECDVRTVYQRD
                     RDRILHSKAFRRMKDKTQVFVAPQGDHYRTRLTHTLEVSQIARTIAKALRLNEDLVEA
                     IALGHDLGHTPFGHAGERALDAVNPDGFAHYKQSVRVAQVLEKNGEGLNLTWEVRDGI
                     LNHRTSGNPSTLEGKVVRLSDKIAYIHHDMDDAQRAGIISEDDIPVTLRMLLGYTTRE
                     RLNTFVHDVIENSLEQDTIKMSAEIYEAMMDLRALMFQNVYENPAAHKEEEKVVKMLT
                     ELYEYYVEYPEAMSKEYRELIVRGEKKEQAVCDYLSGMTDQYSIRKFREIYIPKAWEV
                     Y"
     gene            1339..1423
                     /locus_tag="ACZPD3_05780"
     tRNA            1339..1423
                     /locus_tag="ACZPD3_05780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1373..1375,aa:Leu,seq:aag)
     gene            1561..2829
                     /locus_tag="ACZPD3_05785"
     CDS             1561..2829
                     /locus_tag="ACZPD3_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR1415546.1"
                     /translation="MAFYHMQMQQENIDTGQYQVTVSDRLNNRPIENARVRISYTGAP
                     DSTIEEVTTDSSGRTPVIELKTPPLEYSMEPVEQQPYSEYTIQIEAEGFEPKEIAGSQ
                     VLADTLSRQPTTLNVMESGETFQRIVIPPHTLFYEYPPKIEEAEIKPINENGEIVLSK
                     VVVPEYIVVHDGPVNDSAAGNYYVRYKDYIKNVASSEIYATWPDDTIRANILAIMSFT
                     LNRVYTEWYRNKGYDFTITSSTAYDHKWIYGRNIFASIDRIVDELFENYLSRPNVRQP
                     ILTQYCDGKQVQCRNRGWMTQWGSKALGDQGYSAIEILRTFYGNDMYINVAEAISGIP
                     SSWPGYDLDIGASGNKVRQIQEQLNTIAEAYPAVPVVTADGIYGPETQNSVRIFQSIF
                     GLDQTGIVDYPTWYKIQEIYVAVSRIAELR"
     gene            complement(2946..3068)
                     /locus_tag="ACZPD3_05790"
     CDS             complement(2946..3068)
                     /locus_tag="ACZPD3_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HIR1415547.1"
                     /translation="MNQIRELRYDERLRNEDRNDILAYGIAFCKKKCKVVVENL"
     gene            complement(3158..3298)
                     /locus_tag="ACZPD3_05795"
     CDS             complement(3158..3298)
                     /locus_tag="ACZPD3_05795"
                     /inference="COORDINATES: protein motif:HMM:NF044864.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415548.1"
                     /translation="MSVLAGVHYDNLEKEQLMEVIRILDQLLENLEQELRLRNTKCSQ
                     NV"
     gene            complement(3286..3621)
                     /locus_tag="ACZPD3_05800"
     CDS             complement(3286..3621)
                     /locus_tag="ACZPD3_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR1415549.1"
                     /translation="MIETHLIVTKGAEMTLEQETDYTLEQLYAHADEVHDNYNIGAGP
                     ASGSYRTMGMIVIPCSMKTLAGIVSGYSDNLVLRAADITLKERRKKISLPENGLSRYG
                     NLSNNRCLC"
BASE COUNT         1116 a          985 c          740 g         1209 t
ORIGIN      
        1 agtccaaaat aggtacttcc tcttttgttc agcttcacat actgggatac aacatctaca
       61 atatcattct tcatcctgac ttcttcaatg atatcgtctg gataacgcat gcttcgtcct
      121 ccttcttgat cgattaatag acttcccatg cttttggtat gtaaatctcc cggaatttcc
      181 ttatagaata ctggtctgtc attcctgaga gatagtcaca tactgcctgt tccttcttct
      241 ctccacgcac gatcagctcc ctgtattctt ttgacattgc ttccgggtat tcgacataat
      301 attcatataa ctccgtcagc atcttcacta ccttttcctc ttccttatgg gctgccggat
      361 tctcatatac attctgaaac atgagagccc gcagatccat cattgcctcg tagatctctg
      421 ctgacatctt tatggtatcc tgctcaagac tgttctctat cacatcatga acaaatgtat
      481 tgagccgctc tctggttgta taaccgagca gcatccgaag cgtgaccgga atatcatcct
      541 cagaaataat ccccgcccgc tgtgcatcat ccatatcatg atgaatatag gcgatcttgt
      601 ccgaaagtct tactaccttt ccttcgagag tagacggatt cccactggtt ctgtgattca
      661 ggataccatc ccggacctcc catgtgagat tcaacccttc gccgttcttc tccagtacct
      721 gtgcaaccct gacgctctgt ttatagtggg caaagccatc cggattcact gcatcaagag
      781 ccctctcacc tgcatgtcca aagggcgtat gtcccaggtc atgtccaagt gcgatcgctt
      841 ctacaagatc ttcattcagt ctcagtgcct tcgctattgt tctggcaatc tgtgatacct
      901 caagagtatg tgtcagtctg gtcctgtaat gatcaccctg gggtgccaca aaaacctgtg
      961 tcttatcctt catcctgcga aaagctttac tatgaaggat cctgtccctg tctctctgat
     1021 agacagtccg tacatcgcat tcttcctcct gccgctctct gcctctggaa caaagactca
     1081 aagcagcata aggactgagc atcttttgtt cacgctgttc catgctttct ctgatattca
     1141 taagcgatac ctccacaagc tgacaaaata cctcttatta attatattct acataaaatt
     1201 tcaaattcct ctttttttca aaaaaaaatt gaaatttttt tatatttttt tcaaaaaggt
     1261 gttgacatta gcttttacat ttgatatagt atcacttgtc gcgagggaga gcaaaagaat
     1321 cccaaagaaa tgcgatatgc ggaagtgtcg gaactggcag acgagcaaga ctaaggatct
     1381 tgtgagcatt gcgctcgtgt gggttcaagt cccatcttcc gcatttaaag aatgaggaac
     1441 tatgagaagt catggtgcct tattttttta ttcttttttc tacaataatt cttttctcag
     1501 gtaaccattt tccggtcccc tgcataattt atcaggaaag cattatctac aggagcttgt
     1561 atggcttttt atcatatgca gatgcagcag gaaaatatag atacaggaca atatcaggtt
     1621 accgtatcag accggctgaa caatcgtccc atagaaaacg cacgcgtcag aatctcctac
     1681 acaggagcac ccgacagtac cattgaagaa gtgacaacag attcctccgg acgcactcct
     1741 gtcattgaac ttaagactcc acctctggaa tacagtatgg aaccggttga acaacagccc
     1801 tactcagaat ataccattca gattgaagca gaaggatttg aaccaaaaga aatcgcagga
     1861 agccaggtat tagcagatac tttatccaga cagccaacaa cgctgaatgt catggaatcc
     1921 ggcgaaactt tccagagaat tgtaattcct ccgcatacgt tgttctatga atatcctccc
     1981 aaaatcgaag aagccgagat aaaaccaata aacgaaaacg gcgaaatcgt attaagcaag
     2041 gtcgttgtcc ctgaatatat agtggtacac gatgggcctg tcaacgattc cgctgcaggc
     2101 aattattatg tacgctacaa agattacatc aaaaatgtag ccagcagcga aatctatgcc
     2161 acctggcccg acgacaccat ccgggcaaat attctggcta tcatgtcttt tactctgaac
     2221 agggtttata cggaatggta ccggaacaaa ggttatgact ttacgatcac ttcctccact
     2281 gcctatgatc acaaatggat ctatggcagg aatatctttg ccagcattga ccgcattgta
     2341 gatgaattat tcgagaatta tctttccaga ccaaatgtac gtcagccaat ccttacccag
     2401 tactgtgacg gcaaacaggt acaatgccga aacagaggct ggatgacaca atggggaagc
     2461 aaggcactgg gcgatcaggg atactctgcc attgaaatcc tacgaacttt ttacggaaat
     2521 gatatgtata tcaatgtggc ggaagcaatc tccgggattc cctcctcctg gccgggatat
     2581 gatctggata tcggtgcatc cggaaataag gtaagacaga ttcaggaaca gttaaacaca
     2641 atcgcagaag cttatcctgc tgtccctgtt gttaccgcag atggaattta cggacctgag
     2701 acccaaaatt ccgtcaggat tttccagagc attttcggtc tggaccagac aggaatcgta
     2761 gattatccaa cctggtacaa aattcaggag atctacgtgg cagtcagcag gattgctgag
     2821 ttgcgttagg gttgtagcaa agcagacaaa atatgcgcat cagcctgcaa agaactgatg
     2881 cgcaagaatc tatcacctat ccctttccct tttcagatat catcttttta agattacctc
     2941 attccttata aattttcgac aactaccttg catttcttct tacaaaaagc aattccatat
     3001 gccagaatat catttctgtc ctcattacga agccgttcat cataacgcag ctcccgaatc
     3061 tggttcattg ctttttcaca ggctttttcc agtccgacat aaactcctga tttcgttatc
     3121 tgaaaaatta tagcaccatt gtatttctat gtttctctta tacgttttga ctacattttg
     3181 tattccgcaa tcgcagttct tgttctaagt tttcgagcag ttgatctaat atccggatta
     3241 cttccatcaa ctgttccttt tccagattat cataatgtac tcctgctagc acagacaccg
     3301 gttgtttgac aagttcccat atctgctcag cccattttct ggtaatgata tcttcttccg
     3361 gcgttctttc aaggttatat ctgctgcccg taaaacgaga ttatcactgt acccgcttac
     3421 gattcccgcc agagttttca tactgcatgg aatcacgatc attcccatcg tacggtagct
     3481 tccactcgcc ggacctgcac cgatgttata gttatcatgt acctcgtccg catgagcata
     3541 tagctgttcc aatgtataat ctgtctcctg ttccagcgtc atttccgcac cttttgtcac
     3601 gatcagatgt gtctctatta tttctttata ctgctacagt tgtttcagca attctattgt
     3661 aaggggcgct ccagaagccc cactcatacc gatgataagt ctctttctca tttttcttcc
     3721 tttcctgact tttccgttca tattaaagta tgtttaaaaa attttccaac aatttgcaca
     3781 aattataccc gttcgtttcc tatttccttc gcaattcttc ttttcacatg gattattcca
     3841 acaaggaata cgtaagaaag aatcctgtcc aaaagactgt tacatcacag aaacattgca
     3901 gaataatctt ctatgatata aaaagctccc ctcagtatca caaataataa ttaggtaatt
     3961 gcgaaactcg cggtgctcgt tcgtaacctt gaaccaaaac aaggaagaat tcgcgaagag
     4021 cacgacttgt ggaatgaaaa atggataatc
//
LOCUS       DBMDXG010000171         4049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5215_length_4049_cov_1.297112, whole genome shotgun sequence.
ACCESSION   DBMDXG010000171 DBMDXG010000000
VERSION     DBMDXG010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_4049_cov_1.297112"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            254..664
                     /locus_tag="ACZPD3_05805"
     CDS             254..664
                     /locus_tag="ACZPD3_05805"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR1415550.1"
                     /translation="MVKQIVRDIFFLGQPSKLATKADIQIGKDLQDTLQANRERCVGM
                     AANMIGVRKNIIIVNMGFIDVVMFNPVIVSKHDMYETEEGCLSLDGVRKTTRYQEIEV
                     EYYDFNWKKQRQKLSGWTAQICQHEIDHLSGKII"
     gene            692..1060
                     /locus_tag="ACZPD3_05810"
     CDS             692..1060
                     /locus_tag="ACZPD3_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1415551.1"
                     /translation="MLKNVLIVVDDIEKSIEFYRDLFGLQVILKNEGNVILSEGLVLQ
                     DADIWGKTLNETSTPFNNMMELYFEDFDIDGLLAKYESGKYFVRYATELTELAGGQKL
                     VRLYDPSGNLIEVRTPFRCN"
     gene            1071..1526
                     /locus_tag="ACZPD3_05815"
     CDS             1071..1526
                     /locus_tag="ACZPD3_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HIR1415552.1"
                     /translation="MQILVDADACPVVSIVERVAKEHNLPVTLLCDTNHVLSSDYSEV
                     IVVGAGADAVDYKLISICHKGDIIVSQDYGVAAMVLGKGAYAIHQSGKWYTNENIDQM
                     LMERHLNKKARRASGKNHLKGPRKRTSEDDEHFRESFEKMIHMAMDKKK"
     gene            1560..3089
                     /locus_tag="ACZPD3_05820"
     CDS             1560..3089
                     /locus_tag="ACZPD3_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1415553.1"
                     /translation="MTEKKRTYIAIDLKSFYASVECKERNRDPLATNLVVADKSRTEK
                     TICLAVSPALKCYGIPGRARLFEVVQKVKEANSARRWKAPNRTFIGSSDDSTELNINS
                     ALEIDYIVAPPRMALYLEYSTRIYSIYLKYIAPEDIFPYSIDEVFMDVTDYLHTYNMT
                     PRELAMTMIQDVLKTTGITATAGIGTNMYLCKIAMDIVAKHIKADKDGVRIAELDEMS
                     YRRKLWSHRPLTDFWRVGKGYAKKLEEYGLYTMGDIARCSIGKANELYNEDLLYKLFG
                     VNAELLIDHAWGYEPCTMEMVKAYKPETNSVCSGQVLHCPYDFEKAKLVVKEMTDQMV
                     LDLVDKKLVTDQIVLTVGYDIENLNNADRKKQYHGEVTIDRYGRRIPKHAHGTTNLKR
                     RTSSTKMIMDAVIKLYDRIVDRNMLIRRINITANRLVDESSVEREELYEQLDLFTDYE
                     AQRKKQEEEEAALDREKRMQEAMLNIKKKFGKNAVLKGMNLQEGATARDRNEQIGGHK
                     A"
     gene            3105..3518
                     /locus_tag="ACZPD3_05825"
     CDS             3105..3518
                     /locus_tag="ACZPD3_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415554.1"
                     /translation="MKNNSYDDIINLPHPVSKNHPQMPLRDRAAQFAPFAALTGHDAA
                     IKETARLTDERLELSEEVIAQLNEKINIIRNNIGIEQKVSITYFIPDAKKAGGSYVTC
                     SGIVKKIDEYEHTMIMTDQTVIPIEQISDIKCEEW"
     regulatory      3750..3846
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
BASE COUNT         1358 a          625 c         1003 g         1063 t
ORIGIN      
        1 aactggcaga tgaaaggtta taacacaagc aacggataca tgggatacgt agaaggcaaa
       61 tacattttat ttgccagtga acaggaatat ttcgagttta tgcactacag gggttatatc
      121 tatgttatac ttgagttggt attaaaggat ttagtgagga cttgattgat gctgtaaaat
      181 taatcttgag ccgatgagaa agatttccgg tcaggataaa taagaattag aaattaaata
      241 caggagaaaa taaatggtaa agcaaatcgt aagagacatt ttctttttag gccagccatc
      301 caagctagcc acaaaagcag atatccagat tgggaaggat ctgcaggaca cactgcaggc
      361 gaaccgggaa cggtgcgttg gtatggctgc aaatatgatt ggtgtaagga agaatattat
      421 cattgtgaat atgggattta tagatgttgt tatgtttaac ccggtgattg tttcaaagca
      481 tgacatgtat gagacggaag aaggctgtct gtctctggat ggtgttcgta agacgaccag
      541 atatcaggaa attgaagtag aatattatga cttcaactgg aaaaaacagc ggcagaagct
      601 gtctggatgg acggcacaga tctgtcaaca tgaaattgat catttgtctg ggaagatcat
      661 ataatcgaat gcggattgcg aggtggccgg tatgttaaag aatgtactga ttgtggtgga
      721 tgatatagaa aaatccatag aattttatag ggacttattt ggactgcaag tgattcttaa
      781 gaatgaaggt aatgtgatct tatcggaggg acttgttttg caggatgccg acatatgggg
      841 gaaaacattg aatgaaactt ctacgccgtt caataacatg atggaattgt atttcgagga
      901 ttttgatata gatggattgc ttgcgaaata tgaatctggt aagtatttcg tccgatatgc
      961 caccgaactg acagaacttg caggtgggca gaagcttgta agactgtatg atcccagtgg
     1021 taatcttatt gaggttcgca ctccgttcag gtgcaattaa aggagggaat atgcagattt
     1081 tggttgatgc agatgcctgt ccggtagtca gtatcgttga aagagttgca aaagagcata
     1141 atcttccggt aacgttgttg tgtgatacca atcatgtgtt atcatctgat tacagtgaag
     1201 tgatcgtagt tggagccggt gcagatgcag tggattataa actgatcagt atttgtcata
     1261 aaggcgatat tattgtatca caggattatg gagtggcggc aatggtatta ggaaaaggtg
     1321 cttatgcgat tcatcagtcc gggaaatggt atacgaatga aaatattgac cagatgctta
     1381 tggagcggca tctgaataag aaggcacgaa gggcatccgg aaagaatcat ttaaaaggac
     1441 caagaaaacg aacatcggag gatgatgagc atttcagaga atccttcgag aaaatgatac
     1501 atatggcgat ggataagaag aaataatcac aaacgaaaca caggagaagg aggcgtagaa
     1561 tgacggaaaa gaaaagaact tacattgcaa tcgatcttaa gtcattctat gcgtctgtgg
     1621 aatgtaaaga gcgaaataga gatcctttgg cgaccaatct tgtggttgcg gataaaagca
     1681 gaacagaaaa aacgatctgt cttgcggtat ctccagcatt aaagtgttat gggatacccg
     1741 gaagggctcg tttatttgag gttgttcaaa aagtaaaaga agccaatagc gcccgcaggt
     1801 ggaaagcacc gaacagaaca tttattggtt cttctgatga cagtacagaa ttaaatatca
     1861 attctgcact ggagattgat tatattgttg ctcctccccg gatggctctc tatctggaat
     1921 acagcaccag aatttacagc atttatctga aatatattgc accggaagat atttttccgt
     1981 attcgattga cgaagtattt atggatgtaa cggattatct gcatacttat aatatgacac
     2041 caagagaact ggcgatgacc atgatacagg atgtgctgaa aacgacaggt ataacggcaa
     2101 ctgctggaat cggaacaaat atgtatctgt gtaagattgc gatggatatc gtggcgaagc
     2161 atattaaagc agataaagat ggtgtacgaa ttgcagaact ggatgaaatg tcataccgaa
     2221 ggaaactctg gagtcacaga cctcttactg atttctggag agtcggaaag ggatatgcaa
     2281 agaagttgga agaatacggg ctttatacaa tgggcgatat tgccagatgt tccatcggta
     2341 aagcaaatga gttatataat gaagatctgt tatataagct gtttggcgtt aatgcagagc
     2401 tgctgatcga ccatgcctgg ggatatgaac cctgtaccat ggaaatggtc aaggcttaca
     2461 aaccggaaac gaacagtgtt tgttccgggc aggtacttca ttgtccttat gactttgaaa
     2521 aagcaaaatt agtcgtcaaa gagatgacgg accagatggt tttggatttg gtggataaga
     2581 agcttgtcac agaccagatt gtgctgacag ttggttatga tattgagaat ctgaacaatg
     2641 ccgacaggaa aaagcagtat catggagagg ttacgatcga ccgttatgga agaagaattc
     2701 cgaaacatgc acatggaacg accaatctga aacggcggac ttcctcaaca aaaatgataa
     2761 tggatgcagt aataaagcta tatgacagga ttgttgatag aaacatgctt atcagaagaa
     2821 tcaatatcac agcgaacagg cttgtggatg aaagctctgt agagagagaa gaattgtatg
     2881 agcaactgga tctttttacg gattatgagg cacaaaggaa aaagcaggaa gaggaagagg
     2941 ctgcgctgga tcgcgaaaaa cgtatgcagg aagcaatgct caatattaaa aagaaatttg
     3001 gaaaaaatgc tgtgctgaag gggatgaatc ttcaggaagg tgcaaccgcc agggacagaa
     3061 atgaacagat tggcggtcat aaggcgtaaa ggagttgaaa gaggatgaaa aataattctt
     3121 atgacgatat catcaattta ccgcatccgg tctctaagaa tcatccgcaa atgcctcttc
     3181 gggatcgggc agcgcaattt gctccttttg cagccttgac tggacatgat gctgcgataa
     3241 aagaaacagc aagattaaca gatgaaagat tggaactgag cgaggaagtg attgctcagc
     3301 tgaatgaaaa aatcaatata atcagaaata atatcggtat agaacagaaa gtatcgataa
     3361 cttattttat tcccgatgca aaaaaagccg gcggatccta tgttacgtgc tctggtattg
     3421 taaaaaagat agacgaatat gagcatacca tgatcatgac agatcaaacg gtcattccaa
     3481 tcgaacagat aagcgatatt aaatgtgaag agtggtaaat gtaaaaacag atactcagaa
     3541 gctcgcagtt ttttggatgg cttaccggtg ttggatccgg aatgagaatt gttggaccgg
     3601 aagatgtaaa gcagcagtat aaagaatatc tgcaaagtgt gcttcgaaac tattagttct
     3661 ggcagcagga aaaaattatt ctgaaaagtc agaaaaaacc ttttctatta tcgtattaca
     3721 tggtatactg aataccgaaa tgaatgatat gcaaaatagg attttatgcg tcaagtgttc
     3781 ggtgaatggg gagttgtcac tgaaacgaaa agcacgtctt acggtatatt atccgcaccc
     3841 gttgcaagca ctataagagg gatatctcat attgtgtcag agcctgcccg aaaggagctt
     3901 tctgcaagat gcacattgcg atttgtggaa acgtttttta acaggctcaa gggaatgttt
     3961 tcgacataca ttctatcaca attggagata tcctttttaa tttggtcaaa aggataatct
     4021 ccatttcaaa aatacaaagg aggttattt
//
LOCUS       DBMDXG010000172         4039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5228_length_4039_cov_1.526917, whole genome shotgun sequence.
ACCESSION   DBMDXG010000172 DBMDXG010000000
VERSION     DBMDXG010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5228_length_4039_cov_1.526917"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="ACZPD3_05830"
     CDS             <1..447
                     /locus_tag="ACZPD3_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415555.1"
                     /translation="VGCLPACKSMVNLIMILKCRPMDAGLYQKIHEHQGSLDMAYELY
                     MTFYEKSAYIDAAAVCGNTVVAYSSDPKIDASFMETNSQKIIRKNGYKVTVKIFTDLR
                     PFLERLDSMNDHRESLEEGIKFTPDEKYPDLSRNELIRQTILALCL"
     gene            529..1554
                     /locus_tag="ACZPD3_05835"
     CDS             529..1554
                     /locus_tag="ACZPD3_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR1415556.1"
                     /translation="MKEFIIEENEAGQRFDKYLAKLLREAPKSFFYKMLRKKNITLNG
                     KKATGNEKLLKGDTIKLFLSDETFDKFAGSSQAARAYCELDIVYEDPDIIIINKPAGM
                     LSQPAYDGEPSLVEYLTGYLLKKGDLTEEQLKTFRPSVCNRLDRNTSGMVCAGKSLAG
                     LQFLSRIFHDRTLHKYYICLTKGKIEKPDHIRGYLHKDKKTNKVIVSRQEFKDSLPIE
                     TKYCPLGSNGKITLLEVELITGRTHQIRAHLAGTGHPLLGDTKYGDSEFNKQYIRHGV
                     RHQLLHAYRLVIPETDQTFVAPAPELFCKIIKEENLEEAYHENLERNMGLRKNDHHSS
                     SDRNACE"
     gene            1478..2038
                     /gene="lepB"
                     /locus_tag="ACZPD3_05840"
     CDS             1478..2038
                     /gene="lepB"
                     /locus_tag="ACZPD3_05840"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR1415557.1"
                     /translation="MRIWKEIWDYAKMIIIVVVIVTLVNSVVLINAKIPSESMEKTIM
                     TGDRIFGFRLAYGLNLDFFGHEISKKIKDPERFDIVIFKYPDDESKLFIKRVIGLPGE
                     KVQIKDGKVYINDSEIPLDDSFVPEKPRGSFGPYEVPENSYFVLGDNRNHSKDSRCWK
                     STSFVTFDEIVGKAVIRYYPSVKLLK"
     gene            2064..2597
                     /locus_tag="ACZPD3_05845"
     CDS             2064..2597
                     /locus_tag="ACZPD3_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HIR1415558.1"
                     /translation="MATWNSRGLRGSTLEDLVNRTNEQYREKGLALIQKIPTPITPVR
                     MDKENRHITLAYFEQRSTVDYIGAVQGIPVCFDAKECAADTFPLANIHPHQVEFMQAF
                     EKQGGVAFFLIFYSHENQFYYLTLRSLLTFWNRMQEGGRKSFRREELEPQYYLNKKSG
                     FLVPFLDGIQIDLDERE"
     gene            complement(2766..2897)
                     /locus_tag="ACZPD3_05850"
                     /pseudo
     CDS             complement(2766..2897)
                     /locus_tag="ACZPD3_05850"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            2847..2972
                     /locus_tag="ACZPD3_05855"
                     /pseudo
     CDS             2847..2972
                     /locus_tag="ACZPD3_05855"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            3592..>4039
                     /locus_tag="ACZPD3_05860"
     CDS             3592..>4039
                     /locus_tag="ACZPD3_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1415559.1"
                     /translation="MQRIFHTPEGVRDIYNGECSQKRKVQEKIHRVFHQYGYEDIETP
                     TFEYFEVFSREVGTIPSKELYKFFDREGNTLVLRPDFTPSVSRACATYFSPDKEPVSL
                     CYTGNTFINNTSYRGHLKETTQMGVERIGDESADADAELLAMTVECL"
BASE COUNT         1287 a          870 c          852 g         1030 t
ORIGIN      
        1 gttggctgtc ttccggcatg taaatccatg gtgaatctga tcatgattct gaaatgcaga
       61 ccaatggatg ccggattata tcagaaaatc catgagcatc agggcagtct ggatatggca
      121 tatgaacttt acatgacttt ttatgaaaaa agtgcctata ttgatgcagc agcagtatgt
      181 ggaaatacag tagtggctta cagcagtgat ccgaaaatcg atgcatcttt catggaaacc
      241 aattcccaga agatcatccg taaaaacggt tataaagtga cggtgaaaat ttttacagat
      301 ctccgtccgt tcctggaacg tcttgattcc atgaacgatc acagagaaag tctggaagaa
      361 ggaatcaaat ttaccccgga cgaaaaatat ccggatcttt ccagaaacga actgatccga
      421 cagaccattc tggcattgtg tctgtagtcg ccggaattca attacaaaat atatctatag
      481 caatgtgtta tttcggagtt tggtataatt atacaggaac aattaaatat gaaagaattt
      541 attatagaag aaaatgaagc gggccagcgc tttgataaat atctggccaa gcttctcaga
      601 gaagcaccta aaagtttttt ttacaaaatg cttcgcaaaa agaacatcac tctaaatgga
      661 aagaaagcca caggaaacga aaaacttctc aaaggcgaca ccatcaaact gtttctgagt
      721 gatgaaactt tcgataaatt tgcaggaagc agtcaggcag caagagcata ctgcgaactg
      781 gatattgttt acgaggatcc tgatatcatt atcatcaata aaccggcagg gatgctttca
      841 cagccggcat atgacgggga accgtccctt gtagaatatc tgaccggcta ccttttaaag
      901 aaaggtgatc ttacagaaga acagctcaaa accttcagac cttctgtatg taaccgactt
      961 gaccgtaata ccagcggaat ggtctgtgca ggtaaaagcc ttgccggtct gcagtttctg
     1021 tccaggatat tccacgacag aacacttcac aaatactata tctgcctcac aaaaggcaaa
     1081 atagaaaaac cggatcatat caggggttat ctgcacaaag ataaaaaaac aaacaaagta
     1141 atcgtaagca gacaggaatt caaagattcc ctgccgattg aaacgaaata ttgtcctctt
     1201 ggcagcaacg gaaaaataac tcttctggaa gttgagctga tcacaggccg tactcaccag
     1261 attcgtgctc accttgcagg aacaggacat ccattgcttg gagacaccaa atacggagat
     1321 tctgaattta ataagcaata catccgccat ggagtcaggc atcagcttct ccatgcatac
     1381 aggttagtaa ttccggagac agaccagacc tttgtggcgc cggcaccgga gcttttctgt
     1441 aaaatcatca aagaggaaaa tcttgaggag gcttatcatg agaatctgga aagaaatatg
     1501 ggattacgca aaaatgatca tcatagtagt agtgatcgta acgcttgtga atagcgtagt
     1561 actgatcaat gccaaaattc cttccgaatc aatggaaaaa accattatga caggagaccg
     1621 tattttcgga ttccgccttg catatggact caatctggat ttcttcggac atgaaatatc
     1681 aaaaaaaata aaagatccgg aacgctttga cattgttatt ttcaaatacc cggatgacga
     1741 aagcaagctt ttcatcaaac gagtcatcgg acttccggga gaaaaagtac agattaagga
     1801 cggcaaagtc tatatcaatg attcagagat tcctctggat gacagtttcg ttccggaaaa
     1861 accaaggggc agctttggcc cgtatgaagt tcctgaaaac agctatttcg tcctgggtga
     1921 taatcgcaat cattccaaag actcccgttg ctggaaaagc accagttttg tgacatttga
     1981 tgagattgtc ggaaaggcag ttatcaggta ttatccgtcg gtaaaattat tgaagtagaa
     2041 aataaaattt acggagaaat aaagtggcaa catggaacag cagaggtttg cggggatcca
     2101 ctctggagga tcttgtaaat cgtacaaatg aacaatacag agaaaaggga ctggcactta
     2161 tccagaaaat tcccactccg atcacacctg tccgtatgga taaggaaaac cgtcatatta
     2221 cgctggcata tttcgagcaa cgaagcactg tagactatat cggagcagta cagggtatcc
     2281 ctgtctgctt tgatgccaaa gaatgtgcgg cggatacttt tccgcttgcg aatatccatc
     2341 ctcatcaggt ggaatttatg caggcatttg aaaagcaggg aggggtggct ttttttctta
     2401 tattctacag ccatgaaaac cagttctatt accttacact gcgcagtctc cttactttct
     2461 ggaaccggat gcaggaaggc ggccgcaaga gcttccggcg cgaagaactg gagcctcagt
     2521 actatttgaa taaaaaaagc gggtttcttg tgccctttct ggatgggatt cagattgatc
     2581 ttgatgagag ggaatagaag agggacgcgc agaaatttat tttatttccg gtgcggtttc
     2641 ggcatctaag agtttctaag gcaaaaaaaa tctgctaaaa gcatgagccg tcttctcaaa
     2701 ctcgctccgt cgccgacttc gctcagacag tgaaacagcc ggctcataac gcagattttt
     2761 tttgcctaag aaactctaga gtgtgtctga aaaatcattc ccgcaatctg cacgccccac
     2821 tttgcgggat atttcacccg aattcagttg ccgtagcccg ctacggcgcc ctcatccggg
     2881 caaaatatcc cacaaattgt gacgcacatc ttgcagaaag cctttttcag acacgctcta
     2941 agatacctgc aaatgcaccg gaaataaaat aaatttctgc gctgtgtatt ggcagtaacc
     3001 atgaagaaat caactggctg gcatactata gcaagcctct tataatgtat attgattcag
     3061 ttcatcagag cgtaaaatcc tgcgtcagat atccttgatg tgcgtcagca cgcctgtagg
     3121 tatctgcctt gtctttcgca ctcggctgaa ctgtcttaaa tgcattagtt aatagacttg
     3181 ctatagccgt cataatcttc cacattgcga aaaccagctc ggaaacatat tgtatttgag
     3241 gtgcatttgc aggtgtctta gagattctta gttttcagga aatctgcgtt atgagaagaa
     3301 aagccccttg tctgagcgac agcgagtaat tccgggcttt tcttctcatg gttttagcag
     3361 atttcctgaa gacttagaag ctcttagata ccgaaaccgc acctcaaata caatatgttt
     3421 ctgagcgtcc ctctaaacat ataataataa cattaactgt acttgacatt tttctaaaaa
     3481 acaattataa ttctatctag tttcacatta ctttagtagc acgttactgt actaaagcga
     3541 aatcggcaaa gtaataacaa ccacaataca taaccaagag gaggaccacc catgcagcgt
     3601 attttccaca cccctgaagg tgtaagagac atctacaacg gcgaatgcag ccagaaaaga
     3661 aaggtacagg agaaaatcca tcgcgtcttc caccagtatg gctatgaaga catagaaacc
     3721 ccgacattcg aatactttga agtattcagc cgcgaagtag gaaccatccc atccaaagaa
     3781 ctttataaat tctttgacag agaaggcaac accctggtac tcagacctga cttcaccccg
     3841 tctgtatcca gagcctgtgc aacctacttt tccccggaca aagaacctgt aagcctctgc
     3901 tacacaggaa acacatttat taataacacc agctacagag gacatctcaa agaaaccaca
     3961 cagatgggag tagaacggat cggtgatgaa tccgcagatg cagatgcaga acttcttgcc
     4021 atgacagtgg aatgccttc
//
LOCUS       DBMDXG010000173         4019 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5257_length_4019_cov_2.568657, whole genome shotgun sequence.
ACCESSION   DBMDXG010000173 DBMDXG010000000
VERSION     DBMDXG010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5257_length_4019_cov_2.568657"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            304..2766
                     /locus_tag="ACZPD3_05865"
     CDS             304..2766
                     /locus_tag="ACZPD3_05865"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR1415560.1"
                     /translation="MTNEMFKKEAFKKSVKDNVKFLYRKTIEEATQEQIFQAVSYSVK
                     DVIIDNWLATQKAYDEQDPKIVYYMSMEFLMGRALGNNLINLCAYGEVKEALEELGFD
                     LNCIEDQEPDPALGNGGLGRLAACFLDSLATLNYAAYGCGIRYHYGMFKQKIQNGYQI
                     EVPDNWLKNGYPFELRRPEYAKEVHFGGYVRVEYDPEKGGSKFIHEGYQAVKAIPYDM
                     PITGYDNDVVNTLRIWDAEPIVDFELDSFDKGDYKKAVEQENLARNIVEVLYPNDNHY
                     AGKELRLKQQYFFVSASLQAAIAKYKKKHGDIHKLYEKVTFQMNDTHPTVAVAELMRI
                     LMDEEGLGWDEAWEVTTKSVAYTNHTIMSEALEKWPIELFSRLLPRVYQIIEEINRRF
                     ILEIQAKYPGNYEKIKKMAIIYDGQVKMAHLAIAAGYSVNGVARLHTEILKNQELKDF
                     YEMMPKKFNNKTNGITQRRFLLHANPLLADWITEHIGPDWVTDLPQLKKLAVYADDEK
                     ALQEFMNIKFKNKERLAKYILEHNGVEVDPHSIFDVQVKRLHEYKRQLLNILHVIYLY
                     NQIKMHPEMEFYPRTFIFGAKASAGYATAKKIIKLINSVADVVNNDASINGKIKVVFI
                     ENYRVSNAEWIFAAADVSEQISTASKEASGTGNMKFMLNGAPTLGTMDGANVEIVEEV
                     GAENAFIFGLSSDEVINYENNGGYDPNVIYNTDEEIRQVLMQLINGTFSNDTELFRDL
                     YDSLLNTKNTDRADRYFILADFRSYADAQKRVEAAYRDEKGWAKKALLNTACSGKFTS
                     DRTIQEYVDDIWHLDKVIVRKK"
     gene            3151..3759
                     /locus_tag="ACZPD3_05870"
     CDS             3151..3759
                     /locus_tag="ACZPD3_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415561.1"
                     /translation="MANNTIFDNVFRTMLEKMPELVVPLINEVFGTNYPADIAVEQQR
                     NEHQTKNGEKITDSRLKIADKIYHIECQSTGDAEMVIRMIEYDFAVSLETKQIENGRY
                     KIYFPHSCVLYLRGKGRKDTLGMDIIMPDGRVIKYELPAIYIERYTQDVIFQKKLLFF
                     LPFYVMRYEKSREDIEKDPEKLQKLLDEYVTISRRLEESLLE"
     gene            3870..4019
                     /locus_tag="ACZPD3_05875"
                     /pseudo
     CDS             3870..4019
                     /locus_tag="ACZPD3_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703654.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1432 a          781 c          857 g          949 t
ORIGIN      
        1 ttactgacaa ggtaatgata agttacaggt atttcacagg aaatatacta ctttcgcaat
       61 gcacgataaa aaaatgttat aaatttcggc aattatacac atgacagact gtcgaaaaaa
      121 gagtatcatt atctttgaca gtctgatttc agagaggaaa ttggaggcag agattctttg
      181 cccccaattt ctctgatatg agaaaacaga agagttgacg gaatgaagag gttcaccggc
      241 aatatccgca gggtgtattg ctgagaataa agtgaacagt gaaaccatta cgggaggtat
      301 gaaatgacaa acgaaatgtt caagaaagag gcattcaaga aaagcgtaaa agacaacgta
      361 aaattcctct atcgaaaaac aattgaagaa gctacacagg agcagatttt tcaggcagta
      421 agctactctg taaaagatgt tatcattgat aactggcttg caacacagaa agcttatgac
      481 gaacaggatc cgaagatcgt atactatatg tccatggaat tcctgatggg acgtgctctc
      541 ggaaacaacc tgatcaacct ttgcgcttac ggtgaagtaa aagaagctct cgaagaactg
      601 ggatttgacc ttaactgtat cgaagatcag gaaccggatc cggcacttgg aaacggtggt
      661 ctgggaagac tggctgcatg cttccttgat tctctcgcaa ctttaaatta cgcagcatat
      721 ggctgcggaa tccgttacca ctacggaatg ttcaaacaga aaatccagaa cggttatcag
      781 atcgaggtac cggataactg gttaaagaac ggctacccat tcgaactgcg tcgtccggaa
      841 tatgccaagg aagtacactt cggcggatat gtaagagtag agtacgatcc tgaaaagggc
      901 ggaagcaaat ttatccatga aggatatcag gcagtaaaag ccattcctta tgatatgccg
      961 atcacaggat atgacaatga tgtagtaaac acacttcgta tctgggatgc agagccgatc
     1021 gtagacttcg agcttgattc ctttgacaag ggtgattaca agaaagctgt agagcaggag
     1081 aaccttgccc gcaatatcgt agaagttctt tacccgaacg acaaccacta tgcaggtaaa
     1141 gagctgagac tgaaacagca gtacttcttt gtatctgcaa gcctccaggc agcaattgcc
     1201 aaatataaaa agaaacatgg cgatatccat aaattatatg aaaaagtaac cttccagatg
     1261 aatgatacac atccgactgt agcagtagca gagttaatgc gtatcctcat ggacgaagaa
     1321 ggactcggat gggatgaagc atgggaagta acaaccaaat ctgttgctta cacaaaccat
     1381 actatcatgt ccgaagcact tgagaaatgg ccgatcgagc tcttctccag actgcttccg
     1441 cgtgtatacc agatcatcga agagatcaat cgtcgtttca tccttgagat ccaggcaaaa
     1501 tatccgggaa attacgagaa gatcaaaaag atggcaatta tctacgatgg acaggttaag
     1561 atggctcacc ttgcaatcgc agcaggctat tctgtaaacg gtgttgcacg ccttcataca
     1621 gaaatcctga agaatcagga attaaaagac ttctatgaaa tgatgcctaa gaaattcaac
     1681 aacaagacaa acggtatcac tcagagacgt ttcctgctcc acgcaaatcc actccttgca
     1741 gactggatca cagagcacat cggaccggac tgggttacag accttccaca gttaaagaaa
     1801 ctggcagttt atgctgatga tgagaaagca ctccaggaat tcatgaacat taagttcaag
     1861 aacaaagagc gtctggctaa atacatcctt gagcacaatg gcgtagaagt agacccgcac
     1921 tccatcttcg atgtacaggt taagagactt cacgagtaca aacgtcagct cctgaacatc
     1981 ctgcatgtta tctatctgta caaccagatc aagatgcatc cggaaatgga attctatcca
     2041 agaacattca tcttcggtgc aaaagcatcc gcaggttacg caacagccaa gaagatcatc
     2101 aaactcatca actccgtagc agatgtagta aacaatgacg cttccatcaa cggcaagatc
     2161 aaagttgtat tcatcgaaaa ctacagagta tccaacgctg agtggatctt cgcagcagca
     2221 gacgtttccg agcagatctc cacagcaagt aaagaagcat ccggtacagg taacatgaag
     2281 ttcatgctca acggtgctcc tacacttgga acaatggacg gtgccaacgt agagatcgta
     2341 gaagaagttg gcgcagaaaa cgcattcatc ttcggtttaa gctccgacga agtaatcaac
     2401 tacgaaaaca acggcggata cgatccaaac gtaatctaca acacagacga agaaatccgt
     2461 caggtactga tgcagctcat caacggaacc ttctccaatg atacagaact cttccgtgac
     2521 ctgtacgatt cacttctcaa tacaaagaac acagacagag cagaccgcta cttcatcctc
     2581 gcagacttcc gttcctatgc agacgcacag aaacgtgtag aagcagcata cagagatgaa
     2641 aaaggctggg ctaagaaagc actcctcaac acagcatgca gcggtaagtt cacatccgat
     2701 agaactatcc aggaatatgt agatgatatc tggcatctgg ataaggttat tgtcagaaag
     2761 aaataatacg ctatatttat agaagtaatt gcttctgaat aaaaataaag cccctggcag
     2821 tacagttatc aggactgtat tgcaggggct ttttgctaca gtagtgacat aatacattaa
     2881 ttcatttgtg cgggaaaatt ttgcgtacag acatataata taaaggaaca aaaagttctt
     2941 gaaaacatag atttgactgg aaaaatgaga aggcatctgt tacaatagaa aatacaaaac
     3001 agagtgcaaa gagggtgatc gaaaacaaaa acactttgca caaaagataa aatttcatag
     3061 atgcgaagca tctggaaata agaatacgtc cgaaaacata atcaaaatac aaaagcgaac
     3121 gataactaac agccaggaaa ggtgtatcag atggcaaata acaccatatt tgataatgtt
     3181 ttcagaacaa tgctggagaa aatgccagag cttgtagtgc cgctgatcaa cgaggtattt
     3241 ggcacaaatt atcccgcaga tatagcagtt gagcagcaga gaaacgaaca ccagacaaag
     3301 aacggagaaa agattacaga ttcccggtta aaaatagcag acaagatcta tcatatagaa
     3361 tgtcagagca ccggagatgc agaaatggta atccgaatga tagaatatga ttttgctgtc
     3421 agtctggaaa caaagcagat tgagaatgga cggtacaaaa tatattttcc tcattcctgt
     3481 gtattatatt tacgtggaaa aggcagaaaa gatactcttg gaatggatat tatcatgccg
     3541 gatgggcggg taataaaata tgagttgccg gctatttaca tagaaagata tacacaggat
     3601 gtaatctttc agaagaaact actctttttc ctgccatttt atgtgatgag atatgaaaaa
     3661 agcagagaag atatagagaa agatccagaa aaacttcaga aattgctgga tgaatatgtg
     3721 acaatatcca gaagactgga agaaagtctg cttgaataac tgcgtgtcgc attctcgtta
     3781 ctttcagtga tgagttagac gcgcaaattt ttttactaaa caacagaaca aatgtttaca
     3841 taaaccaata catatggtac aatatttata tggaaaaaga tatatttaat atagataaaa
     3901 acaaactttc atatggtagg gggatatgtc tattccttgc agtatcatct tgtctggtgt
     3961 acaaaataca gaaaaaaggt tttaaaaaac ggaatcgata cagaatgcaa agaaatgtt
//
LOCUS       DBMDXG010000174         4015 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5263_length_4015_cov_1.583035, whole genome shotgun sequence.
ACCESSION   DBMDXG010000174 DBMDXG010000000
VERSION     DBMDXG010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_4015_cov_1.583035"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            303..1538
                     /locus_tag="ACZPD3_05880"
     CDS             303..1538
                     /locus_tag="ACZPD3_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR1415562.1"
                     /translation="MNTLRKIYCRAFQKAFHIAIPFLPYRKPKIAGSVRELPEIIMRH
                     KCTHVLIITDGGIMTLGLTRRLEKALKEAGIPYTIYDKTVANPTTVNVREALELYHKE
                     GCDAIIGFGGGSSMDCAKAVGACAVKPNQSLAQMKGILKVHKKLPLLMAVPTTAGTGS
                     ETTLAAVITDADTRYKYAINDFPLIPRYAVLDPKVTLSLPPFITATTGMDALTHAVEA
                     YIGNSTTIDTRRDALKAVKLIFENIDIAYEHGDNIQARRNMLHASFYAGCAFTKSYVG
                     YVHAVAHSLGGQYNVPHGLANAILLPLVLREYGSCIDKKLHRLAIAAGLADKNTPDHE
                     ATELFIRAIEEMKERFGIVNIVKEIQETDIPKLAHYADKEANPLYPVPKLMDASELEK
                     FYYMLMSLTSKENTSEAEK"
     gene            1653..3032
                     /locus_tag="ACZPD3_05885"
     CDS             1653..3032
                     /locus_tag="ACZPD3_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR1415563.1"
                     /translation="MTETEIKEIVQKQRSYFYTGATLPVEKRLDALKKLKTCIQKYEP
                     LINEALKRDLGKSNFESYMCEVGLVLSELSYMIRHTPSYAKEKTVLTPLAQFASRSYK
                     KPSPYGVVLVMSPWNYPFLLTIDPLIDAIAAGNTVVLKPSAYSPHTSRVIETIITECF
                     DPQYVAVVNGGRAENNTLLNEHFDYIFFTGSQHVGREVMAKASVHLTPVTLELGGKSP
                     CIVEKNANLKLAARRIVFGKYLNCGQTCVAPDYIYCDREIKDELIRQIQKQIRKQFGS
                     TPLNNKNYGKIINEKHFTRICNLIDPSKVVCGGDNNPGALQIAPTVMDNVTFGDAVMQ
                     EEIFGPVLPVLAYDSLDEAIEKVNSMAHPLALYIFTSDKEAAEKVTSHCGFGGGCVND
                     TIIHLATSEMGFGGFGESGMGSYHGKDGFHTFSHYKSIVDKKTWLDLPMRYQPYRKIY
                     EKLLRKFLK"
     gene            complement(3042..3761)
                     /locus_tag="ACZPD3_05890"
     CDS             complement(3042..3761)
                     /locus_tag="ACZPD3_05890"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR1415564.1"
                     /translation="MSERKNRIEARKDELIRRSLLSEEFLEMIQKNKRPFDLLMSYYQ
                     CAVMEIETKFRVLNQEYSLEYDKNPIEGIKTRIKSYDSIVRKIRRKNIPMSLTAIEEN
                     IKDIAGVRVICSFPEDIYELADSFLRQDDIVLIEKKDYIKNPKPSGYRSLHLIVQVPI
                     FLQKNKKMVNVEVQFRTIAMDFWASLEHKLRYKKDIPADQAQQLQEELLACATQSAQL
                     DNRMQEIRNQLVSRADKGNQS"
BASE COUNT         1170 a          934 c          791 g         1120 t
ORIGIN      
        1 tcccttatga ccttttttct tatctgtttt taatatctgg cttccacaaa tttggggtaa
       61 ctcatcacta ctacactatt cgccccagca tagccaggga ttaactcttt catttgtatg
      121 aaaccaagtc tgaaccgcag aaaaagattg gatagttccc tttagtatgc cctgtcctgt
      181 ttatagtttt gaatatcaaa atatattgac ttttcttttt taccttgtta tatttttttc
      241 tgaagtaact gctgtccggc agtctgaaaa acagcaggct gccgtcaaga aaggaattat
      301 ccatgaacac tctcagaaaa atatattgca gggctttcca aaaggctttt cacattgcaa
      361 ttcccttttt accttacaga aaacccaaaa tcgcaggaag tgtcagagaa cttcccgaaa
      421 tcatcatgcg ccacaaatgc acccatgttc tgatcattac agatggcggt atcatgacgc
      481 ttggacttac cagaagactg gaaaaagctt taaaggaggc cggcatcccc tataccattt
      541 atgataaaac agttgccaac cctactactg tcaatgtccg cgaagccctg gagctttacc
      601 ataaagaggg ctgtgatgcc atcatcggtt tcggcggcgg ttccagcatg gactgtgcca
      661 aggcagtcgg tgcctgtgcg gttaaaccca atcagtctct ggcccagatg aaaggaatcc
      721 tgaaggtgca taagaaactt ccccttctca tggcagttcc aaccactgcc ggaacaggaa
      781 gtgagaccac tctcgccgct gttatcacag atgcagacac cagatacaaa tacgcaatca
      841 acgatttccc gctgattccc cgctatgcag tgctggatcc caaggttacc ttaagcctgc
      901 ccccatttat caccgcaact acaggtatgg atgccctgac tcacgcagtg gaggcatata
      961 tcggcaattc caccaccatt gatacacgca gggatgcatt aaaagcggta aaactgattt
     1021 ttgaaaatat agacattgcc tatgagcatg gcgataatat tcaggcaaga cgaaatatgc
     1081 tccacgcctc cttctacgca ggctgtgctt ttaccaaatc ctatgtagga tatgttcatg
     1141 cagtcgctca ctctctgggt ggacagtaca acgttcccca tggacttgcc aatgccattc
     1201 tgctgccgct ggtacttcgg gaatatggct cctgcattga caaaaagctc cacagacttg
     1261 ccattgcagc aggacttgca gacaaaaata cgccggacca tgaagcaaca gagcttttta
     1321 tccgggcaat tgaagagatg aaagagcgat ttggaatcgt aaatatcgta aaagaaattc
     1381 aggaaacaga tatccccaag cttgcccatt atgcagataa agaggccaat cctctgtatc
     1441 ctgtgccgaa gcttatggat gcctccgaac tggagaaatt ttattacatg ctgatgtctc
     1501 tgacttccaa ggaaaatacc tcagaagctg agaagtaact gttaatattc ttctatcctg
     1561 caaagcgttt tttataaaaa tatcaaaata caggaacctg cagactctca atagttacct
     1621 gatatctata ttatataagg aaataatgta ttatgacaga aacagaaatt aaagaaatcg
     1681 ttcagaaaca acgcagctat ttttacacag gtgccacttt accggtagaa aaaaggctgg
     1741 atgccttaaa aaaattaaag acctgtattc agaaatatga accactgatc aatgaagcac
     1801 tgaaacgtga ccttggaaaa agtaactttg aatcctatat gtgtgaagtg ggacttgtgc
     1861 tctcagaact cagctacatg atccgccata caccttccta tgcaaaagaa aaaacagtac
     1921 tgactcccct tgcccagttc gcttccagaa gctataagaa gccatctcct tatggtgttg
     1981 tcctggttat gagcccatgg aattatcctt tcctgctcac cattgatccg ctgatcgatg
     2041 cgattgcagc cggaaataca gttgtactga aaccaagtgc ctactctcct cacaccagcc
     2101 gggtgattga aaccattatc accgaatgtt ttgatcccca gtatgtggct gtggtaaacg
     2161 gcggacgtgc ggaaaataac actcttctca atgaacattt tgactatatt ttctttacag
     2221 gaagccagca tgttggcagg gaagtaatgg caaaagcatc tgtacatctg accccggtta
     2281 ccctggagct gggcggtaaa agcccctgca ttgtagagaa aaatgccaac ctgaaacttg
     2341 cagccagaag aattgtattt ggaaaatatc tcaactgtgg tcagacctgt gtagcacctg
     2401 attatattta ctgcgacaga gaaatcaagg atgaactgat ccgacagatc cagaaacaga
     2461 tcaggaaaca gtttggttcc acacctctga ataataagaa ttatgggaaa attattaatg
     2521 aaaaacactt tactcgtatc tgcaatctga tcgatcccag caaggtagtc tgtggcggtg
     2581 acaacaatcc gggagctctt cagatcgctc ctactgttat ggataatgta accttcgggg
     2641 atgcagtaat gcaggaggaa atcttcggtc ctgtgcttcc tgtgctggcc tatgattccc
     2701 tggatgaggc catcgaaaag gtcaactcca tggcacatcc tctggcacta tatatcttca
     2761 cctccgacaa agaagctgcg gaaaaagtta cttcccactg tggattcgga ggcggatgtg
     2821 tcaatgacac catcatccac ctggcaacca gtgagatggg atttggcggc tttggtgaaa
     2881 gcggaatggg ttcctaccat ggcaaagacg gttttcatac cttctcccac tataagagta
     2941 ttgtagacaa aaaaacatgg ctggatctgc caatgagata ccagccatat agaaaaattt
     3001 atgaaaaatt attgaggaag ttcctgaaat aaggaacttt ctcaggattg attcccctta
     3061 tcagctctgc tgaccagttg attacgaatt tcctgcatcc ggttatccaa ctgagcactc
     3121 tgggtggcac acgccagaag ctcctcctgc aattgctgtg cctgatctgc cgggatatct
     3181 tttttatagc gaagcttatg ttccagactt gcccagaaat ccatggcaat agttcgaaac
     3241 tggacttcca catttaccat ttttttgttt ttctgcagaa agataggcac ctgcacgatc
     3301 agatgcaggc ttctataacc actgggtttt ggatttttta tataatcttt tttctcgatc
     3361 agtacaatat catcctgacg cagaaagctg tctgccaact catagatatc ctccggaaaa
     3421 gaacagatta cccggacacc tgcgatatcc ttgatatttt cttctatagc agttaaagac
     3481 atgggaatat tttttctgcg tattttccgc acaatgctgt cataagattt aattctggtt
     3541 ttaataccct cgataggatt tttatcatat tccagggaat actcctgatt cagcactctg
     3601 aattttgtct caatctccat gacagcacac tgatagtaag acatcagcag atcaaagggc
     3661 cttttatttt tctggatcat ttccagaaat tcttcagaca gcagactcct gcggatcagc
     3721 tcgtcctttc tggcttctat acggtttttt cgttccgaca taatttcttc aaaatttttc
     3781 aaatgttcat ccccctctta cggttttgta taattactat agcagtcctc gtaaataatg
     3841 catttaatag gctcgctata aactgttcac ttttttcagc aacaataaat caaagactat
     3901 tataacacaa ttcccgcaaa gagtaaccat tttcgttact gttcactccg ttcccagcaa
     3961 cacgcttcgc gatgcacaga atttatgcta atcgcataaa ttcgcgcagt atgtc
//
LOCUS       DBMDXG010000175         3942 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5362_length_3942_cov_1.867482, whole genome shotgun sequence.
ACCESSION   DBMDXG010000175 DBMDXG010000000
VERSION     DBMDXG010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5362_length_3942_cov_1.867482"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(20..1243)
                     /locus_tag="ACZPD3_05895"
     CDS             complement(20..1243)
                     /locus_tag="ACZPD3_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR1415565.1"
                     /translation="MDMNNVNIEEIVKQVLSGMTGNAPAAATVSAPAATTGIPKTARV
                     AVLTEKEHFELKEYPIPPIGDDDILVKVEGCGVCGTDAHEFKRDPFGLIPVALGHEGT
                     GEIVAMGKNVKVDTAGKPVKVGDKVVTCMIFKDDPEITMFDLNKKNVGGADVYGLLPD
                     DDVHLNGWFSDYIFLRGGNFGTTFFNVSDLDLDSRILIEPCAVLVHAVERAKTTGILR
                     FNSRVVVQGCGPIGLICIAVLRTMGVEHICAVDGNEKRLEFAKRMGADTSVNFMNFKG
                     IEALTEAVKEAQGGHLADFAFQCTGNPKAHANIYKFIRNGGGLCELGFFINGGDATIN
                     PHFDLCSKEINLVGSWVYTLRDYVTTFDFLKRANAIGLPMSELITDKFPLEQINEALQ
                     TNLAMTGLKIAVVNK"
     gene            complement(1271..2659)
                     /locus_tag="ACZPD3_05900"
     CDS             complement(1271..2659)
                     /locus_tag="ACZPD3_05900"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR1415566.1"
                     /translation="MPINENMVQEIVQEVMAKMQIADAPTGKHGIFKEMNDAIEAAKK
                     SQLIVKKMSMDQREKIITCIRKKIKENAEVMARMGVEETGMGNVGDKILKHHLVADKT
                     PGTEVITTTAWSGDRGLTLIEMGPFGVIGAITPCTNPSETILCNTMGMLAGGNTVVFN
                     PHPAAIKTSIYAINLLNEASLESGGPDNIAVTVEKPTLETSNVMMKHKDIPLIAATGG
                     PGVVTAVLSSGKRGIGAGAGNPPALVDETADIRKAATDIVNGCTFDNNLPCIAEKEIV
                     AVSSIVDELMHYLVTENDCYLASKEEQDKLTEVVLAGGKLNRKCVGRDARTLLSMIGV
                     NAPANIRCIVFEGPKEHPLITTELMMPILGVVRARDFDDAVEQAVWLEHGNRHSAHIH
                     SKNIDNITKYAKAIDTAILVKNAPSYAALGFGGEGYCTFTIASRTGEGLTCASTFTKR
                     RRCVMADSLCIR"
     gene            complement(2689..3552)
                     /locus_tag="ACZPD3_05905"
     CDS             complement(2689..3552)
                     /locus_tag="ACZPD3_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1415567.1"
                     /translation="MVNEYEIKKQICDIGRRIYSRNMVAANDGNISVKLNDNEFLCTP
                     TGVSKGFMTPEYICKVDREGNVIQANPGFKPSSEIKMHMRVYEKRPDVGSVVHAHPIY
                     ATSFAIAGIPLTQPIMPEAVISLGCVPIAEYGTPSTMEIPDNLEKYLPYFDAVLLENH
                     GALTWSTDLNAAYMKMESVEFYAQLLYQSKVLGGPKEFDEKNIEKLYEIRRKFGMAGK
                     HPANLCLNKDGKNCHNCGGACQDGEFKKFPGYQYDFVGGDSKPAENNAASDAQLVAEI
                     TKQVMAQLGMK"
     gene            complement(3742..>3942)
                     /locus_tag="ACZPD3_05910"
     CDS             complement(3742..>3942)
                     /locus_tag="ACZPD3_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HIR1415568.1"
                     /translation="IARFADTLPGVDKIHLLPYHRLGQDKYEGLGRPYLMGNVEPPSK
                     EHMETLKKAVHAVCGLDCQIGG"
BASE COUNT          988 a          910 c          733 g         1311 t
ORIGIN      
        1 tattaccggt gaacagtaac tatttgttta caactgcgat cttaagtcct gtcattgcca
       61 ggtttgtctg aagagcttca ttgatctgct ctaacgggaa tttgtctgta attaactcag
      121 acattggaag accgattgca tttgctctct taaggaaatc aaatgttgta acataatctc
      181 tgagagtgta aacccaggaa ccaaccagat tgatttcttt ggagcaaaga tcaaagtgcg
      241 ggttaattgt agcatctcca ccattgatga agaaaccaag ttcgcaaagg ccgccgccgt
      301 tacggataaa tttataaata tttgcatgag cttttggatt tcctgtacac tggaatgcaa
      361 aatctgcaag gtgtccaccc tgtgcttctt ttacagcctc tgtcagtgct tcgattcctt
      421 tgaagttcat gaagttaaca cttgtatctg ctcccattct cttcgcaaat tcaagacgtt
      481 tctcatttcc gtctactgcg cagatatgtt ctacgcccat agtacgaagt actgcaatgc
      541 agatcagacc gattggtccg cagccctgaa ctacaactct gctgttgaaa cgaaggattc
      601 cggttgtttt tgctctctca actgcatgta caagtactgc acatggctcg atgaggattc
      661 gggaatctag atccagatca cttacattaa agaatgtagt tccgaaattt ccacctctta
      721 agaagatgta atcggagaac catccgttaa gatgtacatc atcatccgga agaagtccat
      781 atacgtcagc tccacctacg ttcttcttgt tcaggtcgaa cattgtgatt tccggatcat
      841 ctttaaagat catacaggta acgactttat cgccaacctt tacaggtttt cctgcagtat
      901 ctactttaac attcttaccc atagccacga tttcacctgt accttcatgt ccaagtgcta
      961 ccgggatcag gccaaatgga tctcttttaa attcatgtgc atctgtacca cacacaccgc
     1021 atccttcaac tttaaccagg atatcgtcat ctccaatcgg aggaatcgga tattctttca
     1081 gttcgaaatg ttccttttca gttaatacag ctactctggc tgtcttcggg attccagttg
     1141 ttgctgccgg tgcagaaact gttgctgctg ccggtgcatt tcctgtcatg ccggagagaa
     1201 cctgtttcac gatttcttct atatttacat tattcatgtc catgttcttt ctctccttaa
     1261 ctcatcatct ttaacgaatg caaaggctgt ctgccattac gcagcgtctt ctcttggtaa
     1321 atgtacttgc gcaggtaaga ccttcacctg tacggcttgc gattgtaaat gtacaatagc
     1381 cttcaccgcc aaatcctaat gcagcatagg atggtgcatt ctttacaagg attgctgtat
     1441 caattgcttt cgcatatttt gtaatattat caatattctt tgaatggata tgtgcagaat
     1501 ggcgatttcc gtgttctaac catactgcct gttccactgc atcatcaaaa tctcttgctc
     1561 ttactacacc aaggatcggc atcataagct ctgttgtgat cagcggatgt tccttcggtc
     1621 cttcaaatac gatacagcgg atatttgccg gtgcattgac accgatcatg gaaagaagtg
     1681 ttcttgcgtc acgtccgaca catttacggt tcagttttcc gcctgccaga actacttctg
     1741 taagtttatc ctgttcttct ttagatgcaa gataacaatc attctcagtt acaagataat
     1801 gcatcaactc atctacgata gaagaaactg ctacgatctc tttctctgcg atacatggaa
     1861 gattgttatc aaatgtacat ccgtttacaa tgtctgttgc cgctttacgg atgtcagctg
     1921 tttcatcaac aagtgccgga gggtttcctg cacctgcacc aatgccacgt ttgccggaag
     1981 aaagaactgc agttacaact ccaggtcccc cggttgctgc aataagcggg atgtctttat
     2041 gtttcatcat tacattgcta gtttcaagag taggtttctc tacagtaacc gcaatattat
     2101 ccggtccgcc ggactctaat gatgcttcat tcagaagatt gatcgcataa atggaagtct
     2161 tgattgctgc cggatgagga ttaaatacaa ctgtatttcc tcctgcaagc attcccattg
     2221 tattacataa aattgtttca cttggattgg tacatggagt aatcgcaccg atcacaccga
     2281 aaggtcccat ttcaataaga gttaatcctc tgtctcctga ccatgcagtt gttgtaataa
     2341 cttctgttcc cggtgtctta tcagctacga gatgatgttt gagaatctta tctcctacat
     2401 ttcccatacc ggtctcttcc acacccatgc gtgccattac ttctgcattt tcttttattt
     2461 tcttacgaat gcaggtaata attttttctc tctgatccat ggacattttc tttacaataa
     2521 gctgtgattt cttggcagct tcaattgcat cattcatttc tttaaaaatg ccatgtttac
     2581 cagtcggagc gtctgctatc tgcatttttg ccataacttc ctgtacaatt tcctgtacca
     2641 tattctcatt aattggcatg agactgtcct ccttgattat ctatgctctt atttcattcc
     2701 taactgagcc attacttgtt ttgtaatctc agcaactaac tgtgcatcag atgcagcatt
     2761 attctctgcc ggttttgaat ctccacctac gaaatcatac tgatatccag ggaatttctt
     2821 gaattctccg tcctgacatg ctccaccgca gttatgacag ttcttgccgt ctttgttcag
     2881 gcagaggttt gccggatgtt taccagccat accgaattta cgacgaattt cataaagttt
     2941 ctcaatgttc ttctcatcaa attccttagg tccgccaaga acttttgact gatacagaag
     3001 ctgtgcgtag aactcaacag attccatctt catgtaagct gcgtttaagt ctgtgctcca
     3061 tgtcaatgct ccatggttct caagaagaac tgcatcgaaa tatggaaggt atttctcaag
     3121 attatccgga atctccattg tagatggtgt accgtattca gcgatcggaa cgcatccaag
     3181 tgagataact gcttcaggca tgatcggctg tgtcagcgga atacctgcaa ttgcaaaaga
     3241 tgttgcataa atcggatgag catgcacaac agaacctacg tccggtctct tctcataaac
     3301 tctcatatgc attttgattt cagaggatgg tttgaatccc gggttagcct ggatcacatt
     3361 tccctctctg tctactttac agatatactc cggtgtcata aaacctttgg atacacctgt
     3421 aggtgtgcaa agaaattcgt tgtcgttcag ttttacagag atgttaccat cgtttgcagc
     3481 taccatgttg cggctgtaga ttcttcttcc gatgtcgcag atctgttttt tgatttcata
     3541 ttcgtttacc atgctcattt tcctccttaa ggcattgata tagtttttga cgtttctacc
     3601 tgtgatttta cactatcggt cataaaaagt caatacattt ccgttgtttt atgttgtttt
     3661 tacaggtttt attgtgttgt ttttgtttgt ttgttttttt ctttttttat tccaataatc
     3721 gtatcagatg caaaaaatac ctcatccacc gatctgacag tccagaccgc agaccgcatg
     3781 tactgccttt tttaatgttt ccatatgttc tttggacggt ggttccacat ttcccatcag
     3841 atatggtctg ccaagtccct catatttatc ctgtccaagt ctgtgatacg gaagaagatg
     3901 aatcttgtcc actccgggaa gtgtgtctgc aaaccttgca at
//
LOCUS       DBMDXG010000176         3934 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5368_length_3934_cov_1.866945, whole genome shotgun sequence.
ACCESSION   DBMDXG010000176 DBMDXG010000000
VERSION     DBMDXG010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5368_length_3934_cov_1.866945"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>3934
                     /locus_tag="ACZPD3_05915"
     CDS             <1..>3934
                     /locus_tag="ACZPD3_05915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIR1415569.1"
                     /translation="IFAGLNVTVTVIEKFRLSGQYNPQNFLETYRSSMELELRSYNML
                     EYNMFRQAQISFPGENDLHMILPDSVIAREKSGILIEYLQKVFCERCGMDLKVELEFR
                     ETQESKYRKNAAVQIAQEVENVIRHAKLNSKNEETAQSEEAGTAEKKAEKKTDKGQQE
                     KKDKKAAFADRKDNRKGDFRGGFRRDSNPDVIYGRDFEGEPVALETITGEMGEVIVRG
                     QVMEVEAREIRNEKTILIFPITDFTDSIVVKMFLRNEQVPEVTEHVKKGAFLKFRGVT
                     TVDRFDSELTIASIAGIKKIANFTTARVDTSPQKRVELHCHTKMSDMDGVTDAKSLVK
                     RAYEWGHPAIAITDHGVVQAFPEANHCFDAWGGCVPKDSDFKVLYGMEGYLVDDLKGM
                     VTNGKGQKLNGRFVVFDIETTGFSSLTCQIIEIGAVLVENGEITDRFSTFVNPKVPIP
                     FRIEQLTSINDSMVMDAPTIEEVLPKFLEFSKDAVMVAHNADFDMGFIMKNCDRLGIA
                     HDFTYVDTVGMARFLLPALNRFKLDTVAKAVGVSLENHHRAVDDAACTAEIFVKFVKM
                     LEERDIRDVDMLNEQGAVSVNTIRKLPTYHVIIFARNETGRINLYKLVSQSHLKYYHR
                     RPRVPKSVLEQYRDGLLVGSACEAGELYQAILRNAPDTEIARLVNFYDYLEIQPVGNN
                     RFMIADDKHDMISSEEDLKEINRKIVKLGEQFKKPVVATCDVHFMDPQDEIYRRIIMA
                     GNGFSDADEQAPLYLRTTEEMLEEFAYLGSEKAEEVVITNTNKIADMIEKISPIHPDK
                     FPPVIENSDQDLKNICFTKAHEMYGDPLPEIVESRLDRELNSIISNGYAVMYIIAQKL
                     VWKSNEDGYLVGSRGSVGSSLAATMSGITEVNPLPPHYLCPNPDCKYSDFDSPEVKQY
                     AGMAGCDMPDKICPKCGTRMKKEGFDIPFETFLGFKGDKEPDIDLNFSGEYQANAHRY
                     TEVIFGKGQTFKAGTIGTLAEKTAFGYVKNYFEERGVHKRYCEINRIVQGCTGVRRTT
                     GQHPGGIIVLPVGEEIEKFTPVQHPANDVNSDIITTHFDYHSIDGNLLKLDILGHDDP
                     TMIRMLEDLTGISARDVPLDQRDVMSLFASTKALKIEPEDIGGCKLGCLGIPEFGTDF
                     AMQMLIDTKPKYFSDLIRIAGLSHGTDVWLGNAQVLIQEGKATISTAICTRDDIMIYL
                     IGKGVESGLAFTIMESVRKGKGLRDEWIETMKEHGVPDWYIWSCKLIKYMFPKAHAAA
                     YVMMAYRIAWYKVFQPLAYYAAYFSIRATAFSYELMCMGKERLEYYMAE"
BASE COUNT         1165 a          713 c         1051 g         1005 t
ORIGIN      
        1 atttttgcag gactgaatgt aacagttaca gttattgaga aattccgcct ttccggtcag
       61 tacaatcctc agaactttct ggaaacttac agatccagca tggaactgga actgcgcagc
      121 tacaatatgc tggaatacaa tatgttcaga caggcgcaga tttcttttcc cggagaaaat
      181 gatcttcata tgatccttcc tgattctgta atagccagag aaaagagcgg gatcctgatc
      241 gaatatctgc agaaggtttt ctgcgaaaga tgcggaatgg acttaaaggt ggaacttgag
      301 ttcagggaga cgcaggaaag taagtaccgt aagaatgcag cagtgcagat cgctcaggaa
      361 gtggaaaatg taatccgtca tgcgaaatta aacagtaaga atgaagagac ggctcagtct
      421 gaagaagcag gaacagcaga gaagaaagca gagaaaaaga ctgataaggg acagcaggaa
      481 aagaaggaca aaaaagctgc atttgcagac cgcaaagaca acaggaaggg agatttccgg
      541 ggtggattca gaagggacag caatccggat gttatctatg gaagagattt cgaaggagag
      601 cctgttgcac ttgagacgat taccggtgag atgggtgagg tgatcgtccg cggacaggtt
      661 atggaggttg aagccagaga aatccgtaat gaaaaaacaa ttcttatctt cccgatcaca
      721 gactttacgg acagtattgt tgtgaagatg tttttgcgca atgaacaggt gccggaagtg
      781 acagaacatg taaagaaggg ggcttttctg aagtttcgcg gtgtgacaac agtagaccgt
      841 tttgacagtg aactgactat tgcatccata gccgggatta agaaaattgc caattttaca
      901 actgctagag tagataccag cccacagaaa agagtagagc ttcactgtca tacaaagatg
      961 agcgatatgg atggggttac ggatgccaaa tctctggtga aacgtgctta tgagtgggga
     1021 catcctgcca ttgccattac agaccatggg gttgtacagg cgtttccgga ggcaaatcac
     1081 tgtttcgatg cctggggcgg atgtgtgccg aaggattctg attttaaggt tctttatgga
     1141 atggaaggct atctggtaga tgatttaaaa ggtatggtga ccaatggaaa aggtcagaag
     1201 ctgaacggta gatttgtggt ctttgatatt gagacaacag gtttttcttc gctgacctgt
     1261 cagatcatag agattggcgc cgtactggta gaaaatggtg agattacgga ccgtttttcc
     1321 acatttgtaa atccaaaggt accgattcct tttcgcattg aacagctgac cagtatcaat
     1381 gacagtatgg tcatggatgc tccgactatc gaagaagtgc ttccgaaatt ccttgaattc
     1441 agcaaggatg cggttatggt tgcacacaat gcggactttg atatgggatt tattatgaaa
     1501 aactgtgaca gactgggaat cgcacatgat tttacttatg tagatacggt cggtatggcc
     1561 cgtttcctgc ttcctgcatt gaacagattt aagctggata cggtagcaaa agcagttggg
     1621 gtttcactgg aaaatcatca ccgggcagta gatgatgccg catgtactgc agagattttt
     1681 gtgaagtttg taaagatgct ggaagagcgt gatatccggg atgtggatat gctgaatgag
     1741 cagggagctg tttctgtaaa tactatcagg aaactgccga cctaccatgt gatcattttt
     1801 gccagaaatg agacagggcg cattaatctg tataaattag tgagtcagtc gcacctgaag
     1861 tattatcaca gacgtccacg tgttcccaaa agtgtgctgg agcagtacag agacggactt
     1921 ctggtgggaa gtgcctgtga agcaggggaa ctgtatcagg caattctgag aaatgcaccg
     1981 gatactgaga tagccagact ggtaaatttc tatgattatc tggagatcca gcctgttgga
     2041 aataacagat ttatgatagc agatgataag catgatatga tcagttccga agaagacctg
     2101 aaggagataa acaggaagat cgttaagctt ggagagcagt ttaagaaacc ggtagttgca
     2161 acctgtgatg tgcatttcat ggatccacag gatgagattt acagaagaat cattatggcc
     2221 ggaaacggat tctcagatgc agatgagcag gcaccgctgt atttacgtac cacagaggaa
     2281 atgctggaag aatttgccta tctgggaagc gagaaagcag aagaagttgt cattacaaat
     2341 acaaataaga tcgcagatat gattgagaag atatcaccta ttcatccgga caaattcccg
     2401 ccggtcattg agaattccga ccaggatctg aagaatatct gttttacaaa agcgcatgaa
     2461 atgtatggag atccattgcc ggagattgta gaaagcaggc ttgacaggga actgaattca
     2521 atcatttcca atgggtatgc cgtaatgtat atcattgcac agaagcttgt atggaagtca
     2581 aatgaagatg gttatctggt aggttcgcga ggttctgtag gatcttctct ggcagctacc
     2641 atgtccggta tcacagaggt aaatcctctg ccgccgcatt atctctgtcc taatccggac
     2701 tgcaaataca gtgattttga ttcaccggaa gtaaagcagt atgcaggtat ggcggggtgt
     2761 gatatgccgg ataagatctg tccgaaatgt ggaaccagga tgaaaaaaga agggtttgac
     2821 attccttttg agacattcct tggattcaag ggagataagg aaccggatat tgaccttaac
     2881 ttttcaggag agtatcaggc taatgcacac agatatacag aggttatttt tggtaagggg
     2941 cagacattca aggcagggac tatcggtacg cttgcggaga aaactgcatt tggttatgtg
     3001 aagaattatt ttgaagagcg tggtgtgcat aaacgttact gtgagatcaa ccgcatcgta
     3061 cagggatgta caggcgtgcg gcgtacaaca ggacagcatc cgggaggcat tatcgtactt
     3121 cctgtaggtg aggagattga gaaatttact cctgtccagc accctgcgaa tgatgtgaac
     3181 agtgacatta tcaccacaca ttttgattac cattctattg acggaaacct gctgaagctt
     3241 gatatacttg gacacgatga tcccactatg atccgaatgc tggaggattt aacgggaatc
     3301 agtgccaggg atgttcccct ggatcagagg gatgtcatgt cattgtttgc aagtacaaag
     3361 gctttgaaaa ttgaaccgga agatattggc ggatgtaagc tcggatgtct gggaatcccg
     3421 gaatttggta cagatttcgc catgcagatg cttattgaca ccaagccgaa atacttctct
     3481 gacctgatcc gtattgcagg gctttctcat ggtacggatg tatggctggg taatgcccag
     3541 gtgctgatcc aggagggtaa ggcaaccatt tccacagcta tctgtacccg agacgatatt
     3601 atgatctacc ttatcggaaa aggagtggaa agtggccttg cgtttacgat catggagagt
     3661 gtccgtaagg gaaaaggtct tcgtgatgag tggattgaga ccatgaaaga gcatggagtt
     3721 ccggattggt atatctggtc ctgtaagctg atcaagtata tgttcccgaa ggcccacgca
     3781 gcagcatacg ttatgatggc atacagaatt gcgtggtata aggtgtttca gccactggca
     3841 tactatgcgg catatttcag tatccgcgcc acagcgttct cctatgagct gatgtgtatg
     3901 ggaaaggaac gcctggagta ttatatggca gaaa
//
LOCUS       DBMDXG010000177         3924 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5387_length_3924_cov_1.950824, whole genome shotgun sequence.
ACCESSION   DBMDXG010000177 DBMDXG010000000
VERSION     DBMDXG010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5387_length_3924_cov_1.950824"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="ACZPD3_05920"
     CDS             <1..639
                     /locus_tag="ACZPD3_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HIR1415570.1"
                     /translation="LAGEHIPVNTGNFTNDMTTFRDENDVLTLLIHLGYLAYDSTEHC
                     VYIPNNEIRNEYANAVSVSDWGEVSKALKNSADVLNAIWNQNEQMVAQGIQRAHFETS
                     HLQYNDENALSYTISLALYAARNYYSVYRELPTGKGFADMVFIPRKKFPDKPALVVEL
                     KWDKSAKGAISQIKQKQYCKSLEEYAGNILLAGINYSKKDKEHQCMIEKIVK"
     gene            962..1546
                     /locus_tag="ACZPD3_05925"
     CDS             962..1546
                     /locus_tag="ACZPD3_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR1415571.1"
                     /translation="MSQSYAKSPAAERKFTTREMVLVGMFAAVLAVISQISLPMPTGV
                     PITIQVFGVALVGAVLGSRLGTTATLVYVLLGAIGLPIFANFSGGISSIVGVTGGYIW
                     AWPIMTWLCGIRPKTENKTKNLAISIGFALIGLLIVETIGGLQWHFVGGSMSIPAIAV
                     YSLTAFIPKDIVITVLAVLIAIPIRKGINNAGNR"
     gene            1530..1913
                     /locus_tag="ACZPD3_05930"
     CDS             1530..1913
                     /locus_tag="ACZPD3_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HIR1415572.1"
                     /translation="MQETADKSNRLKNSLPEDNLVKQENIKIHGIGLDKAGRCTHYHT
                     QLDIAALLCAKCRKYYACYSCHDELEDHSFMATTPEEAYPVLCGNCGRKLTLQEYKKG
                     SCPYCHAGFNPKCSLHENIYFCCTM"
     gene            complement(1892..2047)
                     /locus_tag="ACZPD3_05935"
     CDS             complement(1892..2047)
                     /locus_tag="ACZPD3_05935"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415573.1"
                     /translation="MTNKKRIQNFLLFCILYIRACLKKLSAKYTAKWGVQIAEMFFKT
                     RYIVQQK"
     gene            2155..2937
                     /locus_tag="ACZPD3_05940"
     CDS             2155..2937
                     /locus_tag="ACZPD3_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR1415574.1"
                     /translation="MDINVFYGILIPFLGTSAGAACVFFMKKNLNEQIQRALTGFAAG
                     VMVAASIWSLLIPAIEQSSGLGKLSFVPAAMGFWIGVFFLLLLDHMIPHLHQNSNKAE
                     GPKSKLQRTTMLVLAVTLHNIPEGMAVGVVYAGYLTGHAQITIMGAMALSIGIAIQNF
                     PEGAIISMPLRSEGMGKTKAFAGGVLSGIVEPVGAVLTILAAGLIVPALPYLLSFAAG
                     AMLYVVVEELIPEMSAGEHSNIGTIFFAVGFSLMMILDVALG"
     gene            3083..3520
                     /locus_tag="ACZPD3_05945"
     CDS             3083..3520
                     /locus_tag="ACZPD3_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIR1415575.1"
                     /translation="MDYRKFGECYYIRMDRDDEIISTILEICKKENIRSATFSGIGGC
                     KDAEIQTFIPETGSFEEQRISGMLELVSLNGNVVTGENNICYHHTHAVFSYKDGERHC
                     MAAGHMKSITVLYTTEIELRPVTGGTIHRKYDPETGTGFWDFN"
BASE COUNT         1247 a          720 c          855 g         1102 t
ORIGIN      
        1 ctggcaggag agcatattcc tgttaatacc ggaaatttta caaatgatat gacgacattc
       61 cgggatgaga atgatgtttt gaccttattg atccatctgg gatatctggc atatgattct
      121 acagaacatt gtgtgtatat tcctaataat gaaatccgga atgagtatgc aaatgctgtt
      181 tctgtatcag actggggtga agtgtcaaaa gctttaaaaa attcggcaga tgtcctgaat
      241 gcaatctgga atcagaatga gcagatggtt gcacagggaa tccaaagagc acactttgag
      301 acctcgcatc ttcagtataa tgatgaaaat gcattaagtt atactatttc actggctctg
      361 tatgcggcta gaaactatta tagcgtatat cgtgaactgc caacaggaaa gggatttgcg
      421 gatatggtct ttattccgag aaaaaaattt cctgataaac ctgctctggt agtggaatta
      481 aaatgggata aaagtgccaa aggagctatt tctcagatta aacagaagca atattgtaaa
      541 agtctggagg aatatgcagg aaatattctt ttggcaggga ttaattattc gaagaaggat
      601 aaagagcatc agtgcatgat tgagaagata gtgaaataac aacgctatat atgatttatt
      661 gttactgttc acagcaacac gctttgcgat gcaggaaata tgaacctcct tgtgcaatgc
      721 ccaaggagta tcatttgctt cgcaaactta atttatgcta atcgcataaa ttcgcgcggt
      781 atgtctcggg attttggcat attcatgcca aaacacctcg cgggacagtg gtgtgtgaac
      841 agtaacgact tattacaaat gtaaacccaa aattgaagaa cgggttgaca atcttctctg
      901 cttatgatat aaaaaagaag gtcaaaatca atcattaaca gggaaagagg agagtcttat
      961 tatgagtcag tcatatgcaa aaagccctgc tgcagagaga aaatttacaa ccagagaaat
     1021 ggtactggtt ggtatgtttg cagcagttct tgcagtaata tcacagattt cactgccaat
     1081 gcctaccggt gtaccgatca ccattcaggt atttggtgta gcacttgtag gcgcagtact
     1141 tggttccagg cttggaacta cagcaacact ggtttacgtt ctgttaggag ccatcggtct
     1201 tccgatcttt gctaacttct caggcggaat cagcagcatc gtaggagtta caggaggata
     1261 catctgggca tggccgatca tgacctggct ctgcggaatc cgtccgaaaa cagagaacaa
     1321 gacaaagaat cttgcaatct caatcggatt cgcactgatc ggacttctga tcgttgagac
     1381 aatcggtggt ctgcagtggc actttgtagg aggctccatg agcatcccgg caatcgcagt
     1441 atactcctta acagcattta ttccaaagga tattgtgatc acagttcttg cagtactgat
     1501 cgcaattccc atcagaaaag gaatcaacaa tgcaggaaac cgctgataaa agtaacaggt
     1561 taaagaatag cctgccggaa gacaatctgg taaagcagga aaacataaag attcacggaa
     1621 tcggacttga taaagcagga cggtgtacac actatcacac acagcttgac atagctgcat
     1681 tgctctgcgc gaagtgcagg aaatattacg catgttattc ctgccacgat gaactggaag
     1741 atcattcatt tatggcaaca acaccggaag aagcttatcc tgttttatgc ggaaactgcg
     1801 gcaggaaact tacacttcag gaatacaaaa aaggcagctg cccttactgc cacgcaggat
     1861 ttaatccaaa atgcagttta catgagaata tttatttctg ttgcactatg taacgtgttt
     1921 tgaaaaacat ttccgcaatc tgcacgcccc actttgcagt atattttgca gaaagctttt
     1981 tcaaacacgc tctaatgtag agaatacaaa ataacaaaaa attttgtatt ctctttttat
     2041 ttgtcaaaaa atctgatata ctgattagag ttagaataga ctaactaaat caaacaaata
     2101 acaagaacct atttctttta tttgcataca aataaaaaca ggaggaataa attgatggat
     2161 ataaatgtat tttatggaat tttgattcct tttcttggaa cttctgcagg agctgcctgc
     2221 gtatttttca tgaagaagaa tctgaatgaa cagattcaaa gggcacttac cggctttgct
     2281 gcaggagtta tggttgcagc ttccatatgg agtctgctaa tcccggcaat tgaacagtct
     2341 tccggactgg ggaagctttc atttgtaccg gcagcaatgg gattttggat aggggtgttt
     2401 tttttgctct tgctggatca catgatcccg catcttcacc agaacagtaa caaggcagaa
     2461 ggtccgaaga gcaaacttca gagaactacc atgctggttc ttgcagtaac tctgcataat
     2521 attccggagg gaatggcagt aggagtcgta tacgcaggct atttaacagg tcacgcccag
     2581 atcactataa tgggagccat ggctctttct atcgggattg ccattcaaaa ctttcccgag
     2641 ggagccatta tttctatgcc cctgcgttct gagggaatgg gaaaaaccaa agcctttgca
     2701 ggaggagtcc tgtccggaat cgtagaacct gtcggtgcag tcctcacaat cctggctgcc
     2761 ggcctcattg tcccggcact tccatatctt ttaagcttcg ctgccggagc tatgctttat
     2821 gtagtggtag aagagctcat acctgaaatg tctgccggag aacactccaa cattggaact
     2881 attttctttg cggtaggatt ctctctgatg atgattctgg atgtagctct tggataaaca
     2941 gataataata cactataaat attcattact atagcaatcc ctgccagaga tttttgggga
     3001 ttgttatctg cagggaacag gaatataata tttctgttaa gcaatattta ttcaaaagca
     3061 ataacttaaa tggagataaa aaatggacta cagaaaattc ggcgaatgct attacataag
     3121 aatggatcgt gatgacgaga ttatcagtac aatcctcgaa atatgtaaaa aagaaaatat
     3181 cagatcagcc actttttccg ggatcggagg atgtaaggat gcagaaattc agacctttat
     3241 cccagagaca ggcagcttcg aagaacagcg tatttccgga atgcttgagt tggtatcttt
     3301 aaacggcaac gtggtcacag gtgaaaataa tatctgttac catcatactc atgcagtatt
     3361 ctcttataaa gatggtgaac gacattgcat ggcagcaggt catatgaaat caattactgt
     3421 actgtatact acagaaatcg aacttcgtcc ggtaacaggc ggaactatcc accgcaaata
     3481 cgatcctgaa acagggactg gattctggga ttttaactaa accttagaaa acgatttgtt
     3541 ttgattgagc aaggaagttt tgtgcaaacc ttcaaactga tgtaagataa gattactcag
     3601 tttggaggtt ttatttttat caggtatcag aggtaaatgg atgggtagaa gataaaatgc
     3661 aaaaggaaca gttcattgag agtgtacaga atttaacaca agttctgtat acttttttta
     3721 taagaaatga aactttttgt tccctggcag gatatataag aatgtaaagg ttaaaagctt
     3781 ttaatttttg aaagtattaa aaagaaagtt gggttgatat gacagatgaa gcagagcttt
     3841 tgcagcgtct caggaacaga gaaaaaaatt caatagatga agcaatccga atttatacac
     3901 cgtatttgag tactgttttg tatc
//
LOCUS       DBMDXG010000178         3916 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5402_length_3916_cov_1.530013, whole genome shotgun sequence.
ACCESSION   DBMDXG010000178 DBMDXG010000000
VERSION     DBMDXG010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5402_length_3916_cov_1.530013"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="ACZPD3_05950"
     CDS             complement(<1..193)
                     /locus_tag="ACZPD3_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III N-terminal
                     domain-containing protein"
                     /protein_id="HIR1415576.1"
                     /translation="MEKDFFDVFPSLKVKKELEELLDMVFVTRVSCNPSRTHIWVYIK
                     SERWIHKKHIFELEEQIERQ"
     gene            complement(240..1292)
                     /gene="ispG"
                     /locus_tag="ACZPD3_05955"
                     /gene_synonym="gcpE"
     CDS             complement(240..1292)
                     /gene="ispG"
                     /locus_tag="ACZPD3_05955"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR1415577.1"
                     /translation="MYRDHTKVIQIGDRKIGGGNPILIQSMTNTKTEDVEQTVAQILA
                     LEQAGCDIIRCAVPTMEAAKALKEIKKQIHIPLVADIHFDYRLAIAAMENGADKIRIN
                     PGNIGSTERIKAVVDVAKERGIPIRVGVNSGSLEKELVEKYHGVTAEGLVESALDKVH
                     IIEDLGYDNLVISIKSSDVLMCAKAHELIAEKTNYPLHVGITEAGTLYSGNIKSAIGL
                     GIILNQGIGDTIRVSLTGAPLEEIKSAKRILKTLGLRKGGIEVVSCPTCGRTQIDLIG
                     LANKVETMVQDIPLDIKVAVMGCVVNGPGEAKEADIGIAGGVGVGLLIKHGEIIKKVP
                     EDQLLDTLREELLSWK"
     gene            complement(1344..>2153)
                     /locus_tag="ACZPD3_05960"
     CDS             complement(1344..>2153)
                     /locus_tag="ACZPD3_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIR1415578.1"
                     /translation="FDVQEEGKFFGFFRKQTSKIENLKNKYDKAQANVEKITDSLQQH
                     QVRLMKDSAMLDKMYEQNLNYFKELTMYILAGKKKLEETRNGKLAEMKNKAALSGLPE
                     DAQAARDLDEKCSRFEKKLHDLELTRTIAMQTAPQIRLIQNNDTVMVEKIQTTIVNTI
                     PLWKSQMVLALGIAHSAEAAQAQRQVTDITNELLRKNAETLHMATVETAKESERGIVD
                     LETLQKTNADLIQTLDDVMRIQMEGRQKRQAAEMEMHRMEEELKRKLLEIR"
     assembly_gap    2154..2163
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2164..2481)
                     /locus_tag="ACZPD3_05965"
     CDS             complement(<2164..2481)
                     /locus_tag="ACZPD3_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIR1415579.1"
                     /translation="MGNEFKDFEMETPSLTLEPDLGEFEKKEEVLPKKQTQKEEVPVL
                     TPEEQKMVNDFAAKIDIENTNQILQYGAGTQKKMADFSDTALENVKTQDLGEIGELIS
                     NVVG"
     gene            complement(2516..3814)
                     /locus_tag="ACZPD3_05970"
     CDS             complement(2516..3814)
                     /locus_tag="ACZPD3_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIR1415580.1"
                     /translation="MSENFDELGELGDKIKKIIDTAVSTKDYRQMTEDIKQTVGQTVN
                     SAVNSAVDSGSEAIKNGLNNVFGTGNSQSGDSGVYRNKTKEFEEKRRREREQEQERQR
                     IEKEKAKEKMLTLYERNTGGRMKGMMIAVSGGILASGMGLGTLVLSIFGAVGHMSSLV
                     TGGTCFMAVGALTGVGLLAGGIKKLGKLERFQKYIDTLGNHTYCNFEQLSAAVNKPVK
                     FVKKDIKKMIDDRWFRQGHIDEQETCLITSNETYLQYTQTQKALEQKKQEEEKHQAEQ
                     ERNRKNTPPEVQEVLDKGNEFLDKIHRSNDAIPGEEISAKISRMELIVEKIFERAQKH
                     PEIIPDLKKLMNYYLPMTVKLLDAYEEMDQQPVQGENIQASKKEIEDTLDTLNQAFEK
                     LLDSAFQDTAWDVSSDISVLHTLLAQEGLTDDDFAKMKKN"
BASE COUNT          883 a         1007 c          667 g         1349 t
ORIGIN      
        1 tctgtctctc tatctgttct tccagttcaa agatatgctt cttatgaatc cagcgttcac
       61 tttttatata cacccatata tgagttctgg aaggattgca ggacactctg gtcacaaaaa
      121 ccatgtccag cagttcttcc agttctttct tcacttttaa gctgggaaat acgtcaaaaa
      181 agtctttttc caaaataaaa tctcctcgtt gatctgacag gaagtcttcg ttccaaatgt
      241 tacttccagc tcagaagttc ttcccgcaac gtatccaaaa gctgatcttc cggaactttc
      301 tttatgatct caccatgttt gatcagaagt cccacaccaa ctccgcctgc aattccgata
      361 tctgcctctt ttgcttctcc gggtccgttt accacacagc ccataactgc tactttaata
      421 tcaagcggga tatcctgcac catggtttct accttatttg caagaccgat cagatcgatc
      481 tgtgtgcgtc cacaggttgg acatgacaca acttctatgc cacctttgcg aagccccaga
      541 gttttcagga tacgttttgc ggatttaatc tcttctaaag gtgctcctgt aagagagaca
      601 cgaatggtat caccgattcc ctgattcagg atgatcccca gtccgatagc agatttaatg
      661 tttccggaat aaagagttcc ggcttctgtg attcctacat gaagtggata attcgtcttc
      721 tccgcgatca gttcatgagc ctttgcacac ataaggacat ccgaagattt gatgctgatc
      781 accagattgt catatcccag gtcttcgatg atatgaactt tatccagcgc actctccacc
      841 agtccctctg cagtgactcc atgatatttc tccacaagtt ctttctcaag agaaccgctg
      901 tttacaccaa cacgaatagg tatccctcgc tcctttgcca catcaaccac tgctttgatg
      961 cgctctgtac tgccgatatt tccgggattg atccgaatct tatctgcacc gttttccatg
     1021 gctgcaatgg caagacgata atcaaaatgg atatctgcca caagtggtat atggatctgt
     1081 tttttgattt ccttcagtgc ttttgcagct tccatagtcg gtactgcaca acggataata
     1141 tcacagccag cctgctccag tgccaggatc tgagctactg tctgttctac atcttcagtt
     1201 tttgtatttg tcatggactg gattaaaatc gggttgccgc cgccaatctt tctgtctccg
     1261 atctgaatca cttttgtatg atctctgtac ataattttcc tcttctctca tttatagcaa
     1321 ttccataaat tactgctgaa cagttatctg atttccagaa gttttctctt cagttcttct
     1381 tccatgcgat gcatttccat ctctgctgcc tgtctcttct gacggccttc catctggatc
     1441 cgcatcacat catccagagt ctggatcaaa tctgcatttg tcttctggag tgtttcaagg
     1501 tcaactattc cacgctctga ttctttcgct gtctcaaccg ttgccatatg aagtgtctct
     1561 gcattctttc tgagaagttc attggtaata tctgttacct gtctctgtgc ctgtgccgct
     1621 tctgcagagt gagcaattcc cagagcaagc accatctgac ttttccataa tggaattgtg
     1681 tttacgatcg ttgtctgtat tttctcaacc attaccgtat cattattctg gatcagccgg
     1741 atctgaggcg ctgtctgcat ggcaatcgta cgggtaagtt ccagatcatg cagtttcttt
     1801 tcaaatctgc tgcatttctc atccaggtct ctggctgcct gcgcgtcttc gggaagcccg
     1861 ctcagagctg ccttattttt catttctgca agctttccgt ttctggtttc ttccagtttc
     1921 ttttttccgg caaggatata catggtaagt tctttaaaat aattcaggtt ctgttcatac
     1981 atcttgtcca gcatagcaga atctttcata aggcgcacct gatgctgctg aagagaatct
     2041 gtgatcttct ccacattggc ttgtgctttg tcatatttgt ttttcagatt ttcaatctta
     2101 gaagtctgct ttctgaaaaa tccaaagaat tttccctcct cctgtacatc gaannnnnnn
     2161 nnntcccaca acattactga tcagttctcc aatctcaccc agatcctgtg tctttacatt
     2221 ctcaagagct gtatcagaaa agtccgccat ttttttctgg gttcctgcac catactgaag
     2281 aatctgatta gtattctcaa tatcaatctt tgcagcaaaa tcatttacca ttttctgttc
     2341 ttccggtgtc agtacaggca cttcttcctt ctgtgtctgc tttttgggga gcacttcttc
     2401 tttcttttca aactctccaa gatccggctc cagtgttaat gatggtgttt ccatttcaaa
     2461 atctttaaat tcgtttccca tgtcctctgc cgctcctttt tatccttttt tattttcagt
     2521 tctttttcat ttttgcaaag tcatcatctg tcagaccttc ctgtgcaagc agcgtatgaa
     2581 gcactgaaat atcacttgaa acatcccacg cagtatcctg aaaggcagaa tccagaagtt
     2641 tttcaaatgc ctgattcagt gtatccagcg tatcctctat ctcctttttg gatgcctgga
     2701 tattctctcc ctgcacaggc tgctgatcca tttcttcata tgcatccagc agctttactg
     2761 tcatgggcag ataataattc atcagtttct tcagatccgg aataatctcc ggatgcttct
     2821 gcgcccgttc aaaaatcttc tccacaatca gttccattct ggatattttt gcagaaatct
     2881 cctctccagg aatcgcatcg ttgctcctgt ggatcttatc cagaaattca tttcctttat
     2941 ccagcacctc ctgcacttca ggcggtgtgt tctttctgtt tctctcctgt tcagcctgat
     3001 gtttttcttc ttcctgtttc ttctgctcaa gtgccttctg cgtctgcgta tattgcagat
     3061 aagtctcatt actggtgatc agacaggttt cctgttcatc aatatgtccc tgacggaacc
     3121 atctgtcatc aatcatcttt ttaatatctt ttttaacgaa tttcaccggc ttatttactg
     3181 ctgctgacaa ttgttcaaaa ttacagtatg tgtgatttcc cagtgtgtca atatactttt
     3241 gaaaacgttc cagtttaccc agttttttaa tgccgcctgc gagaagtccc actcctgtca
     3301 aagctccgac tgccataaaa caggttccac cggtcacaag ggaactcata tgccccaccg
     3361 ctccaaagat gctaaggacc agagttccca gtcccattcc actggcaagg attccacctg
     3421 aaacagcaat catcatacct ttcattcttc ccccggtgtt cctttcataa agagtcagca
     3481 tcttctcttt tgctttctcc ttctcaattc tctgacgttc ctgctcctgt tcacgttccc
     3541 gcctgcgctt ttcttcaaat tcttttgttt tattgcggta taccccggaa tcaccggact
     3601 gactgtttcc ggttccaaat acattattca gaccattttt gattgcctcg cttccggaat
     3661 ctacagcaga atttactgcg ctgttaacag tctgtcccac cgtctgctta atatcctcgg
     3721 tcatctggcg gtaatcttta gtagaaactg ctgtgtctat gatctttttt attttgtctc
     3781 caagttctcc taattcatca aaattctcgc tcatattttc ctccattttc tattccatat
     3841 tatagcgaaa tcctctataa aaaacaagac tggcggattt tttgttactg tatgtgaaca
     3901 gtaacacgat ttttca
//
LOCUS       DBMDXG010000179         3903 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5424_length_3903_cov_1.339821, whole genome shotgun sequence.
ACCESSION   DBMDXG010000179 DBMDXG010000000
VERSION     DBMDXG010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5424_length_3903_cov_1.339821"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..732)
                     /locus_tag="ACZPD3_05975"
     CDS             complement(181..732)
                     /locus_tag="ACZPD3_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HIR1415581.1"
                     /translation="MLEKSVIEHCSPTLASIKTGNLFTYKYESEEELWKSVEGFNECV
                     REKGVSLTVLRRSEKKALIYVCRFSSLERDLKKPGVANFIKKYGYESTDPAYALERLR
                     SRLAQREDFPHEIGLFLGYPLGDVIGFIKNAGQNCKCVGCWKVYCNECEAIKAFARFK
                     KCTSVYVRLWNQGRTVRQLTVAA"
     gene            complement(1151..3871)
                     /locus_tag="ACZPD3_05980"
                     /pseudo
     CDS             complement(1151..3871)
                     /locus_tag="ACZPD3_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
BASE COUNT          862 a          978 c          642 g         1421 t
ORIGIN      
        1 tgctccaata tacaactgct actttactca tgattaaaat cctccttaaa tttgctctgc
       61 ctttttcttt gccgcaaagc catgatttat attataataa aaagtcatac ggcttttcta
      121 ttatactgat tcttaaacga actctccatc cctgctatat tactgtatat agcttcttcc
      181 ttacgctgcc accgtcagct gacgtacagt tcttccctga ttccagagac gtacatacac
      241 gctggtacat ttcttaaatc tggcaaaagc ctttattgcc tcacattcat tgcagtaaac
      301 cttccaacaa cctacacatt tacagttctg ccctgcattc ttgataaatc cgatcacatc
      361 tcccagtgga tatcccagaa acaatccgat ttcatgagga aaatcttctc tctgggccag
      421 gcggcttctc agtctctcaa gtgcatatgc aggatctgta ctctcatacc catacttctt
      481 tatgaaattc gcaacaccag gtttcttcag atctctttca aggcttgaga accggcagac
      541 ataaatcagc gcctttttct cactcctgcg aagtactgtg agggaaacgc ctttttctct
      601 tacacattca ttaaagcctt ctacgctttt ccataattct tcctcagatt catatttata
      661 tgtaaaaaga tttccggttt taatagatgc taatgtaggt gaacaatgtt cgatcacaga
      721 tttctccaac atgttatgat ccctttctca atggcaaaat attagttagg ccacactaac
      781 tatctatcaa aaaataaagc tttacgaaac attttattgc atttgtttgc tgcaaagctt
      841 gaattactgc tgtaaaaaga ccttcaacag atcgttcagc agttcgagtt atttatgtct
      901 aacttcataa cttaatatat actatctctg gtattctgtc aagttaattt tgtataactt
      961 tttttagtaa ctgccttttt taaccgaatc aagtaagtcc ccggggtcgg ttgcaaaaaa
     1021 caataagcaa tgggtaggga cttgcttgta tcaggaggag tgacaactcc ttctgataca
     1081 ctgtgaaaca gttattcgat gtagcgccgc atagcaaagc ggatgatttt atccgcttga
     1141 ccataaaatc tcatccttct atacagatcg tacttccaac tccatcttct gtccgtttct
     1201 ttgttactat gatcttctta tcaatacgtt ctttcagctc tgctacatgg gaaacaatcc
     1261 ccaccagtct gcttccctga ctgagatcct tcaatgcgcg gattgcctga tcaagggcat
     1321 cttcatccag agatccgaaa ccttcatcca caaacatggt atcaagctgg attccgccgg
     1381 agcttgactg aatttcatcg gaaagtccca gtgccagaga cagagatgcc tgaaatgttt
     1441 ctccgcctga taaggtcttg acacttcgaa cagttccatt gtaatggtct actacatcca
     1501 gttccagacc ggttttccct accctgctct tgttttcttt tctgcgaact aactcgtact
     1561 gaccgctgct catggtcatc agacggatat tggcacgcgc aagaatacgg tcaaagtact
     1621 gcatctggat gtaagtttcc agcatgatcc ttgccttacc tgtaatcgta gcattggcag
     1681 tgtctgagag agatttcatc cacttccaac gggtttccgt tttcagaagt tctgtctgct
     1741 gtctgcggat tttttcatac atgtcactgt tgatttccag acgggcatgg acctcatcac
     1801 gattttctga aagtgatttc ttttgctgtg caaagttttc tttctgaaca ttcagttcct
     1861 cagctgaaat acttatggat tcttttaact gttctgtcag agatgcaatt accgaatcca
     1921 gttctgtcat tctcttttct aatacttctc tcgcttctgt tactgttttg taatgtattt
     1981 caagcttctt ttgttcctca gtcttaagct ggatctgaga caggatttct tcctttgtcc
     2041 tttcacccag ctctttttcc atattctctc tctgcttctg cagctgctca tatctggatt
     2101 tctctgctgc tgtctgctgc tgattctgat tgatctggtc tgtgatcgtt gtaagttctg
     2161 tctgagtctt accaatctgc atctccagaa gttttctctg ttccagaaat gctgtaagat
     2221 ccttttgttt ctgtcccaga atattttgct gctgtttcag ccattctgct gctctctttc
     2281 cctgcagcaa agcctgtgat acattacaga tttccttttg atacactatt tcaccgctgt
     2341 aatcctgtga cagttccgga tcagttttca ttctctgtct tatatcttct tcatggatcc
     2401 atgtcagtac aggttcgtcc aattgattct gcagctgttc ctgaatactt tccagctggc
     2461 tcttcctctt ttcaagactg atctgtaatt tctgaaattc ttttgcatgt tgttctgact
     2521 gtgtgatgaa ctcttccttt ttcttcagta aatcctgatg aacttttttc tgttttttga
     2581 tttcttccaa tcctgtctgt acactgcttt cctgattttt tacaaataaa cataattcat
     2641 gatcaggaag ttcctttcga agtatctctt gtgtctcctc ttctggcaga aactgcgcta
     2701 tataaccttt aaccccttct gtcagttctt ccctgcagcg ctgcatcagt cctgctgcct
     2761 ctcctgcctg cacacttgcg tcagatgccg ctttttccgt agcttctgtt aactttttct
     2821 gttttttcag ttgttcttct gtgggaactt ccttcggagt ctgcgtaagc tttggatgat
     2881 gaacagaacc acataccgga cacggcattc catcctgaag tcctgctgcc aatatccccg
     2941 cctgcccatc cagataagcc tgttccattt tgttcaggat ttcttttatt tctgcccgtt
     3001 tctgagaagc ggtcagataa gccttctttt tgctttctgc tttcttttcc agatcctgat
     3061 accgtttctg ctgatcttct attttctgta tctgctgcaa acgtacgacc aggctgttct
     3121 ctttgatttc cagattccca ccggaaacaa ttttctcttc cagatcagtc aaatcctttt
     3181 ttatctgttt cgcaagttgt tcttcctgcc cggactgtat tttcagttca ctgatttttg
     3241 tctgaagaaa ctccagattt ccacagttct tataaattcc tgcataaaga gtatctattt
     3301 tctctttccg gaaatctgtt tctgcttttt ctttttctgt accgtctgtt gtctgatatt
     3361 ctttttgttt ttcttcaagc acagcgttca tttctttctg acgattctgc agctctgtac
     3421 cgtccgttcc cagtttctct atctgcaggg aagccttctc tgtttctctg agcagatttt
     3481 ccagatttgt ataacgctcc agattctctt tttctttctg tatggaaagc atcaggcgtt
     3541 gaatctcctc aggttccttt gccccatttt ctgcagcttt ttgtgcctgt tccaactgcg
     3601 gaagcaatac ctctttctct tgcagaagct gttccttcct tgcctttgca tctgcttctt
     3661 tatgagcctt tcccaaaagc tgatttactt tttccagttc tttatcgttc tctttgatct
     3721 gcttattcaa atttttcagc gtctctttat ctaccttcag aaactgctcc aggatttcca
     3781 gaccttcttc cagatttccc tcaaagccat tcttctccat caatatccac tgttgtccca
     3841 actggtcttc tgtagaatat ctgacctgtt ccgccaccgg cagcccgaaa tgcaatttca
     3901 cgc
//
LOCUS       DBMDXG010000180         3895 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5437_length_3895_cov_2.336321, whole genome shotgun sequence.
ACCESSION   DBMDXG010000180 DBMDXG010000000
VERSION     DBMDXG010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5437_length_3895_cov_2.336321"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(92..1342)
                     /locus_tag="ACZPD3_05985"
     CDS             complement(92..1342)
                     /locus_tag="ACZPD3_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415582.1"
                     /translation="MSNKGMIIRECEHLNSARRVLEGRIDCDMFFRSICGDLLITGME
                     AGNKEMVLSLVRACMEKRDVSTIVLTSHQELAALIQQDQNRRKSGAVRVSWPAEKNYH
                     PFYGMSAQQMLRFVCMTAEEMGYSSMAGQVMIYAAAVLNIVSTEYPVSLPAVVKLLEE
                     DDDFISELALQQGLSNVIADNIRANHEAGIVFRRLLERLEEIFENIYVTGSDTKYNLQ
                     SGCKEKLDGMVIYASSGSQRIFNAYLKEEIFQTLKDVPKIRILLDEMEFADENDELMR
                     YIMQLKRQGRIELIVISQNVKESMKGNADLDFANVVLFQHGTPTATEEISKALFGTYQ
                     YHKPMPNAVKPPALLFTFQRAVNWQIQSEERLRVRSEDLSARPGIFGRQSDYLAVKTT
                     ANNNIYFIHTADFLKNTGYGLTVV"
     gene            complement(1362..2780)
                     /locus_tag="ACZPD3_05990"
     CDS             complement(1362..2780)
                     /locus_tag="ACZPD3_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HIR1415583.1"
                     /translation="MSYDFGKKKIGAGEKQERNGGMYSKTLLGQSIVENVVPSWTADG
                     ENRPFVVFRDPDTGNVFGLSKDMLAYGFLAIGAPGTGKTNIFNICLKYLLNAQGERDV
                     IVIFDTKGDYLETFGSQIPDSQKIVIGNGEKYRKITSYHNIFAEVMPRGIDGKLVYTT
                     ESDEDALELCEQLFQTMNSETQPIFPAMAEQVVAALMIYYMRTYWRSDQSKLNNQEFI
                     HFLAGSTNEDLKKILELDYMSDWRSCQDYISGKSNQTQGVNSYFGALLRKMFVGPFAK
                     SDPSKEFSMREIITGGKKKVVFIEYDLRKGQLLAPIYGILLDRALAHALGGRQAVKGN
                     TYFLLDEALQLPKLQHFSDALNFGRSENVKMLCGLQNTAGLEERYGEAGAKNILSSFQ
                     NMAAFRTNDFDTRQFVIHRLGENYQNFSFSAQQENLNIQRAGHTVEDWNLLRLRNGDA
                     VVALAGEKPFIFRMPLYREIKQ"
     gene            complement(2795..3874)
                     /locus_tag="ACZPD3_05995"
     CDS             complement(2795..3874)
                     /locus_tag="ACZPD3_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415584.1"
                     /translation="MGEYGVHPTRYGEVSDGHLYSMFRESAYARLSESEKLDLLQEVV
                     NRDAMEKGEIGAPEVRFAELPVNESGNAADGVIQVNRDMAVSGVQTFEYKGQTVQHRM
                     DDYNCQTLNTILHENMHCLQDQIIDGTIQIDDLQLTSEYQANSYTNSIVFQNGKGQWG
                     SQYMTGETANGYYMYYFQATERDAYRTAEEKTASILQELSGKYGTEASFEAYTKSMAA
                     TGYQAMEQKAILEFQNPNFEKDLNQVLQNQYFGTNVEVDKNTEETVKAEMIATYQNLY
                     EQLGESTAANEDAKMSFDPRPVSLEEYNQAVQNPAAADEEVYEEAEETAEKGPEADTQ
                     SQQESEEVSVSDEDIADGMDSDMEI"
BASE COUNT          953 a         1053 c          646 g         1243 t
ORIGIN      
        1 ttctgttgct tttttcctgt aagagacatc tcatctttat atagagaaat aaaaatctaa
       61 ccttaaccgt tttcagcttt tatttttgat atcagacaac cgtcagacca tatcccgtat
      121 tcttcaggaa atctgctgta tgaatgaaat aaatattgtt gttcgccgtt gtttttacag
      181 caagataatc tgactgcctt ccaaaaatcc ccggacgtgc agaaagatcc tcgcttctca
      241 ctctgagcct ttcctcactt tgaatctgcc agttcacagc cctttgaaaa gtaaaaagca
      301 atgcaggcgg ttttaccgcg ttcggcatcg gtttatgata ttgatatgtt ccaaacagag
      361 ccttcgaaat ctcttccgtt gcagtcggtg ttccatgctg aaacagaaca acattggcaa
      421 aatccagatc tgcatttcct ttcatggatt cttttacatt ctgtgatatt acgatcagtt
      481 caattctgcc ttgtcttttt aactgcatga tgtatctcat cagctcatca ttttcatctg
      541 caaactccat ctcatcgaga agaatcctga ttttcggtac atccttcaat gtctgaaaaa
      601 tctcctcctt cagataagcg ttaaaaatcc gctgactgcc ggagctggca tatatcacca
      661 ttccatcaag tttctcttta cagccgcttt gaagattata ttttgtatcg cttcccgtca
      721 cataaatatt ttcaaatatt tcttccagcc gctccaataa ccgccgaaac acaatgcccg
      781 cttcatggtt tgcccgaata ttatctgcaa taacattgga aagcccctgc tgtaaagcca
      841 gttctgatat aaaatcatca tcctcctcaa gaagctttac gactgcaggc agtgagactg
      901 gatattctgt cgaaacaatg ttaagtaccg ctgccgcata aatcattacc tgcccggcca
      961 tactgctgta tcccatttct tctgctgtca tgcagacaaa ccgaagcatc tgctgggcag
     1021 acattccgta gaaaggatga taattttttt ctgcaggcca ggacaccctg acggcaccgc
     1081 ttttccgcct attttgatcc tgctgtatca acgctgccag ttcctgatga ctcgtgagga
     1141 caatggttga aacatccctt ttttccatgc acgccctgac caatgacagc accatttcct
     1201 tatttcctgc ttccattcct gttatcagaa gatcaccgca gatggaacga aaaaacatgt
     1261 cacagtcaat gcggccctcc aatacccgtc ttgcagaatt taaatgttca cactctctga
     1321 tgatcattcc cttattactc atcgttttcc cccttacgca ctcactgttt tatctctctg
     1381 taaagcggca ttcggaatat aaacggtttt tcacctgcca gcgccactac agcatcacca
     1441 ttccttagtc tcaataaatt ccagtcctcc acggtatgcc ctgctctctg aatattcaga
     1501 ttttcctgct gtgcagaaaa actgaaattc tggtaatttt ctcccaatcg atgaatcaca
     1561 aactgcctgg tatcaaaatc atttgttcga aacgctgcca tattctgaaa agaagaaaga
     1621 atattttttg ctccggcctc cccataccgc tcctcaagac cggctgtatt ctgcagaccg
     1681 cacagcattt tgacattctc cgaacgtcca aaattcaaag catccgagaa atgctgcagt
     1741 ttaggaagct gcagtgcttc gtctaacaga aaatatgtgt ttccttttac cgcctgtctg
     1801 cctcctaatg catgggcaag cgcacggtca agaagaatcc catagatcgg cgccagaagc
     1861 tgaccttttc tcaaatcata ttcaatgaat actactttct tctttcctcc tgtaattatt
     1921 tcccgcatgg aaaattcctt cgatggatct gattttgcaa atggacctac aaacattttt
     1981 ctcagcagcg ctccaaaata ggagttcact ccctgtgtct ggttgctctt gcctgaaata
     2041 taatcctgac agctgcgcca gtctgacata taatctaatt ccagaatttt cttcaggtct
     2101 tcgttcgtgc tgcctgccag aaaatggata aattcctggt tgttcagttt tgactgatct
     2161 gaccgccagt atgttctcat ataataaatc ataagtgctg ctactacctg ctctgccatt
     2221 gccggaaaga tcggctgtgt ttcgctgttc atcgtctgaa acagctgctc acataactcc
     2281 agggcatcct catccgattc tgtcgtataa accagttttc cgtctattcc gcggggcatt
     2341 acctctgcaa aaatgttatg atacgatgta attttccgat acttctctcc atttccaatg
     2401 acaatcttct gactgtcggg aatctggctt ccaaaagtct ccaggtaatc tcctttcgta
     2461 tcaaaaataa cgatcacatc tctttctccc tgtgcattca gcagatactt cagacagata
     2521 ttaaaaatgt tggtttttcc tgttcccggc gcaccgatcg ccagaaaacc atatgccagc
     2581 atgtccttac tcagtccgaa aacatttccg gtatccggat cccgaaaaac cacaaatggt
     2641 ctgttttctc catctgccgt ccaggatgga acgacgtttt caactatgct ctgtcccagc
     2701 agcgtcttac tatacattcc cccgtttctt tcctgttttt ctccggctcc aattttcttt
     2761 tttccaaaat catatgacat cgctttctcc tccttcatat ctccatatca ctgtccattc
     2821 catcggcaat atcctcatcc gatacactta cctcttcact ctcctgctgg ctctgtgtat
     2881 ctgcttccgg acctttttct gccgtttctt ctgcttcttc atacacctcc tcatccgccg
     2941 ctgccggatt ttgtactgcc tgattatatt cctccaatga cactggcctt ggatcaaatg
     3001 acattttcgc atcttcgttt gctgctgtac tttctccaag ttgttcataa agattctgat
     3061 acgttgcaat catttccgct ttaacagtct cttctgtgtt tttatctact tctacatttg
     3121 ttccaaaata ctggttctgc aaaacctgat tcagatcttt ttcaaaattg gggttctgaa
     3181 attccaaaat tgccttttgt tccatcgcct gatatcccgt tgcggccatc gactttgtat
     3241 atgcctcgaa agacgcctct gttccatatt tgccggacag ctcctgcaga atagatgctg
     3301 tcttttcttc cgctgtacgg tacgcatccc tctctgtggc ctgaaaataa tacatgtaat
     3361 atccatttgc tgtttccccc gtcatatact ggcttcccca ctgacccttc ccattctgga
     3421 aaacgatact gttggtataa ctgttcgcct gatattcact ggtcagctgc aaatcgtcaa
     3481 tctggattgt tccatcaata atctgatcct gaaggcaatg catattttca tgcagtatcg
     3541 tgttcaatgt ctggcagtta taatcatcca ttctgtgctg aacagtctgc cctttgtatt
     3601 caaacgtctg aactccactg accgccatat cacgattcac ctgaatcaca ccatctgctg
     3661 catttccgct ttcatttacc ggaagctctg caaaccgtac ctccggggcg cctatctccc
     3721 ccttctccat tgcatctctg tttactactt cctgcagaag atccagcttt tcactttccg
     3781 aaagccgggc ataagcggat tcgcggaaca tagaatacag atgcccgtca cttacttccc
     3841 catatctggt tggatgtact ccgtattctc ccataacaga ctacctcctc atatt
//
LOCUS       DBMDXG010000181         3882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5457_length_3882_cov_1.506744, whole genome shotgun sequence.
ACCESSION   DBMDXG010000181 DBMDXG010000000
VERSION     DBMDXG010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_3882_cov_1.506744"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..1841
                     /locus_tag="ACZPD3_06000"
     CDS             3..1841
                     /locus_tag="ACZPD3_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR1415585.1"
                     /translation="MKIEKPARYIGGEVNSVMKNKDKVEVRFAMCFPDVYEIGMSNLG
                     MMILYNMFNEREDVWCERVFSPWMDLDKIMREEHIPLFALESQEPVKEFDFLGITLGY
                     EMCYTNVLQVLDLSHVSLLAKDRKEDDPIVIGGGACAYNPEPIAEFFDMFYIGEGETV
                     YDALFDAYKANKAAGGSRADFLFAASQIPGIYVPSLYNVTYKEDGTIASFTPAKEGVP
                     EKVCKQLITDVTKDYRAIKAPVVPFIKATQDRVTLEIQRGCIRGCRFCQAGMIYRPTR
                     ERNVEDLKASAREMLQNTGHEEISLSSLSSSDYSELKELVNFLIEEFHGNAVNISLPS
                     LRIDAFALDVMSKVQDVKKSSLTFAPEAGSQRLRNVINKGLTEENILHGAGEAFKGGW
                     NQVKLYFMLGLPTETEDDMKGIAHLAQKIAETYYEEVPKEKRNGKVQVNVSTSFFVPK
                     PFTPFQWAGMYREEDFVEKAKVVKSEIRAQLNQRSIRYSWHEPDVTILEGFLARGDRR
                     CSKVILRAYEKGAIYDAWSESFDYNIWKESFAETNTDIDFYTLRERSTDEILPWDFID
                     AGVTKKFLIHEWEQAKKETVTPNCRQKCSGCGAMRYGGGVCYEGKN"
     gene            1825..2532
                     /locus_tag="ACZPD3_06005"
     CDS             1825..2532
                     /locus_tag="ACZPD3_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR1415586.1"
                     /translation="MKVRIKFSKEGPVKFVGHLDTMRYFQKAIRRANLPVAFSGGYSP
                     HMIMSFAAPLGVGTESLGEYFDLELAETVPTSEITRRLDAVMVEGVHVLSTRQVEDGK
                     AGKAMSLVAAADYYVEFRPGKEPEISWKDKISDFLAQPEIKVMKKTKRSEKEIDIRPF
                     IYKMELQGDKIFMMLASASANYTKPELVTDTFFSWLGIELPEFAYSIKRLEVYADKGT
                     EEEHKFVTLEALGEEVE"
     gene            2516..3730
                     /locus_tag="ACZPD3_06010"
     CDS             2516..3730
                     /locus_tag="ACZPD3_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR1415587.1"
                     /translation="MKKLSKLIITQMNIRQIPCYVCAYEEDGKVMELRFEPVGEKSSL
                     GNIYVGQIENIAANIGAAFVQISAGEKCYLQLSDAPNAIYASVKKGDRPLKAGDEILV
                     QISREAMKGKLPAVTTNLNFTGKYLVLTTGDKKFGLSSKLSNDDRSRISKWMEAEVNR
                     SDKEFGIIVRTNAADASKEEILKELEYLKGLYHKAAVDGRSRTCFSCVYRTEPFYISA
                     VRDANSRNLEEIITDIPEISRQISDYLNSNSPEEKEKLRFYDDKLLPLYKLYRLETVL
                     EEIQHEKVWLNSGGFLVIQQTEAFVSIDVNSGKFTGKKKMQETYRKINLEAAKEIARQ
                     LRLRNLSGIILIDFINMENQDHQDELFHVLQKYLRKDPVKAKAVDITPLHILELTRKK
                     VRKPVIEEIREL"
BASE COUNT         1187 a          763 c          927 g         1005 t
ORIGIN      
        1 tattgaaaat cgaaaagccg gcccgttaca ttggcggtga ggttaattct gtgatgaaga
       61 ataaagataa agtggaagtc cgctttgcca tgtgcttccc ggatgtttat gaaatcggta
      121 tgtccaatct gggcatgatg atcctctata acatgttcaa tgaacgtgag gatgtctggt
      181 gtgagcgtgt cttttctccg tggatggatc tggataaaat tatgcgtgag gaacatattc
      241 ctctctttgc cctggaatcc caggagcctg taaaggagtt tgatttcctt ggtattaccc
      301 ttggatatga gatgtgctat accaatgtgc ttcaggtact tgacctgagc catgtttcac
      361 ttctggcaaa ggatcgtaag gaagatgacc ctatcgtcat cggcggcggt gcatgtgcat
      421 ataatccgga accgatcgca gaattttttg atatgtttta tattggcgag ggtgagactg
      481 tctatgatgc tctgtttgat gcatataagg caaacaaggc agcaggcggc tccagagcag
      541 acttcctgtt tgcagcttcc cagattcccg gaatttatgt tccctcttta tacaatgtaa
      601 cctacaaaga agacggcacc attgcttcct ttacacctgc aaaagagggc gtacctgaga
      661 aagtctgtaa acagctgatc accgatgtca ctaaagacta ccgcgcgatc aaagctcctg
      721 tagttccatt tattaaagct actcaggatc gtgttaccct ggagatccag cgtggctgta
      781 tccgcggatg ccgtttctgt caggcaggta tgatctaccg tcctaccaga gagcgcaatg
      841 tggaggattt aaaggcaagt gccagagaaa tgcttcagaa tacaggacat gaggagattt
      901 ccttaagttc tctaagctcc agtgattatt ctgagctgaa ggaactggta aacttcctga
      961 tcgaagaatt tcatggcaat gctgtcaata tctctctgcc atccctgcgt atcgatgctt
     1021 ttgccctgga tgttatgagc aaggtacagg atgtaaagaa gagcagcctt actttcgcac
     1081 cggaagcagg ctcccagcgt ctgcgtaatg ttatcaacaa aggtctgact gaagagaata
     1141 ttcttcatgg tgcaggtgaa gcattcaaag gtggatggaa tcaggtaaag ctttacttca
     1201 tgctcggact tccaactgaa acagaagatg atatgaaagg tattgctcat cttgctcaga
     1261 aaattgcaga aacttattat gaagaagtgc cgaaagaaaa acgtaatggc aaggttcagg
     1321 tcaatgtaag tacctctttc tttgtaccga aaccatttac cccattccag tgggcaggta
     1381 tgtacagaga ggaagatttc gtggagaaag caaaggtcgt aaaaagcgag atccgcgcac
     1441 agttaaacca gagaagtatc cgctacagct ggcatgagcc ggatgtaacg attcttgaag
     1501 gtttccttgc aagaggtgac cgccgttgtt caaaagttat ccttagagca tatgaaaaag
     1561 gtgcgatcta cgatgcatgg tcagagagtt ttgactataa tatatggaaa gaatcttttg
     1621 cggaaaccaa cacagatatt gatttctata ctctcagaga acgttccaca gatgagatcc
     1681 ttccatggga tttcattgat gcaggagtga ccaagaagtt cctgatccac gaatgggaac
     1741 aggcaaagaa agaaaccgta acaccaaact gccgtcagaa atgttccggc tgtggtgcta
     1801 tgcgttatgg cggaggtgta tgttatgaag gtaagaatta aattcagcaa agagggtcct
     1861 gtgaaatttg tgggacatct ggataccatg cgttatttcc agaaggctat ccgcagagcc
     1921 aatcttccgg ttgctttttc cggtggatac agtccgcata tgatcatgtc ttttgccgca
     1981 ccattaggag ttggtacgga aagtctggga gagtacttcg atctggagct ggcagagaca
     2041 gttcctactt cagagatcac aagacgtctg gatgcagtga tggtagaagg ggtacatgta
     2101 ctcagtaccc gtcaggttga ggacggaaag gcgggtaagg caatgtcact ggtggcagca
     2161 gccgattatt atgtagaatt tcgtccggga aaagagcctg aaatttcctg gaaagataaa
     2221 atcagtgatt tcctggcgca gccggagatc aaagtaatga aaaagaccaa acgaagcgaa
     2281 aaggaaattg atattcgccc gtttatctat aagatggaac ttcagggaga caagatcttt
     2341 atgatgcttg cttccgcaag tgccaattac acaaaaccgg aactggtaac agatacattc
     2401 ttctcatggc ttggcataga acttccggaa tttgcttatt ccatcaaacg tctggaagta
     2461 tacgcagata aaggaacaga ggaagaacat aaatttgtaa ctctggaagc actgggtgaa
     2521 gaagttgagt aaactgatta ttacccaaat gaatatccgg cagattccat gctatgtatg
     2581 tgcgtatgaa gaagacggta aagtaatgga actgcgcttc gaacctgttg gggagaaatc
     2641 ttctcttgga aatatttatg ttggacagat agagaatata gctgccaata tcggtgctgc
     2701 attcgtacag atttctgccg gggaaaaatg ctacctgcag ctttccgatg ctccaaatgc
     2761 gatttatgct tctgtaaaaa aaggtgaccg cccattaaag gcaggagatg aaatccttgt
     2821 gcagatcagc cgggaagcaa tgaagggaaa actaccggct gtaaccacga atctgaattt
     2881 tacaggcaaa tatctggttc ttacaaccgg cgataagaaa tttggtctgt cttccaaact
     2941 ttccaatgat gacagaagcc gtatcagtaa gtggatggaa gcagaagtta accgttcgga
     3001 taaagagttt ggtatcatcg ttcgtacaaa tgcagcagat gcatcaaaag aagaaatact
     3061 gaaagaactg gaatacctga agggcctgta tcataaggct gctgtagacg gaagatcccg
     3121 tacctgtttc agctgcgtat atagaacaga gcctttttat atctctgcag tccgcgacgc
     3181 caacagccgg aacctggaag agatcataac agatatccct gagatatccc gacagatttc
     3241 tgactatctt aacagtaaca gcccggagga aaaagagaag ctgcggtttt atgatgataa
     3301 acttctccct ctttacaaac tttatcgcct ggaaactgtc ttagaagaaa tccagcatga
     3361 gaaagtctgg ctgaacagtg gtggatttct ggtaatccag cagacagaag ccttcgtatc
     3421 cattgatgta aacagcggaa agtttacagg caaaaagaaa atgcaggaaa cttaccgaaa
     3481 aatcaatttg gaggcagcaa aggaaattgc cagacagtta cgtctcagaa acctgtccgg
     3541 aattattctg attgatttta ttaatatgga aaatcaggat catcaggacg aactgttcca
     3601 tgtcctgcag aaatatctgc gcaaagatcc ggtaaaggca aaagcagtgg acatcactcc
     3661 actgcatatc ctggaactta ccagaaagaa agtgcgaaaa cctgtgattg aggaaatcag
     3721 agaattatag tttaccataa aataattatg taaaccaaac aagcatctgc aattctcatc
     3781 gatatttttc cgtaactgaa taactgctct gcagtttgta ttttatcaca gatcctgacg
     3841 gataccggat tgaaattgta cctgtaaaat aaaagaaaaa aa
//
LOCUS       DBMDXG010000182         3882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5458_length_3882_cov_1.483470, whole genome shotgun sequence.
ACCESSION   DBMDXG010000182 DBMDXG010000000
VERSION     DBMDXG010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5458_length_3882_cov_1.483470"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="ACZPD3_06015"
     CDS             <1..1406
                     /locus_tag="ACZPD3_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415588.1"
                     /translation="TRYLGKEISYDDCRKLGDFVVNVILSNEGRAMYFDGYDFEENDY
                     HVMHISYVANRIVYLDQEVRRTSYYLTDDGYNLILSTLEIENNMKLTIHEMIFQMHLE
                     KQSYDKAVDEIKNVFNLMRIQLQKIQEAMGKIRRNALNYSVKDYEEILLENLDTISDT
                     KEKFQKYRELVRSRVKKLEEENINVRRLGEKEEENLENLRIIESYLNRTIDEHQKILS
                     SHFDLKALYTRELEALSQMSLIRRFSMRNDFYDKVLENPSALGNLDYFLRPLFNREPD
                     KVYNLNKALLYQKPSVRNEDEDTEEMLDFDEDAYLKEQEEKRRQKLKRYESSLGFLLE
                     QASEKGEISLSDIWKNIQENEKNAEEMQQQLIPNVEIFKEIMVELIRNKEINMEVLKK
                     ERSEFIQDQTADFQLNEMLLQLSEERFSDKKIGKIEVYRIEDGSTVTFDNVFSENGVK
                     KSIRCSNILIRIMRNEE"
     gene            1412..2152
                     /locus_tag="ACZPD3_06020"
     CDS             1412..2152
                     /locus_tag="ACZPD3_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6063 family protein"
                     /protein_id="HIR1415589.1"
                     /translation="MAYEAEEIRTSQEIFYYLLEHHELREEDDQLLYKAYTEEESIRN
                     LVKSQGEIAGSNVERYGNVIYLIPKEENNFLGFSKQQLKGTLCKSGATDKDYYLSQFV
                     ILTLLVEFFDGQGSSSKSREYMRVGELMNCLSARLKEGAAEETTTEEEEDGKNPKDTA
                     GISFKILYETYEALKSDDRGSRMKTTKEGFIYNILMFLQKQGLIEYVVQDEMIKTTRK
                     LDNFMDWNLLNQNNYQRVLRVLGVAKDE"
     gene            2145..2600
                     /locus_tag="ACZPD3_06025"
     CDS             2145..2600
                     /locus_tag="ACZPD3_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415590.1"
                     /translation="MSKINAVRLINVNYNNNAYRISDETLHFNGKSTLISLQNGGGKS
                     VLVQMLTAPFVHPKYRNTKDRLFESYFTTNKPSFILVEWALDQGAGYVLTGLMVRKSQ
                     DMEEDRKENLDIIGIVSEYQSPCIQDIHHLPVVEKGKKEMILKNFNSCR"
     gene            2631..>3882
                     /locus_tag="ACZPD3_06030"
     CDS             2631..>3882
                     /locus_tag="ACZPD3_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415591.1"
                     /translation="MKFFYYDLTNYAQSRQYFNKLMEYQINYKEWETIIKKINLKESG
                     LSDLFADCKDEKGLTEKWFLEAIESKLNKDKNRIKEFQSILEKYAGQYKDNRSKIKRR
                     DTIRQFKEEANGIQTQAEEYQNAETEEGKQQNKIAWFIHDVNGLLSQTEKAHDHAKEV
                     LEGLDQKLSRVEYEELSSQVYDYEEEKNLHIGNRDMIDMERENLQVQAEQTEKKLHIL
                     QCARQQDSVSEEKGELDLLREKIAVARQQGADLEPKRKALGLTLKNIFEERIQDNRQQ
                     QENLSENIQKKGKEAQDEANRAEELEEKIRDCFGKKGKLESRIESYTRLENQYNAKYQ
                     EELVRNILGTYEAGTLEIRRQMYEQELEKTVRERTENQKKWEDDKELIRRQERSLEDK
                     KEALIHKKTETENQEHTYQLYQNEL"
BASE COUNT         1367 a          687 c          949 g          879 t
ORIGIN      
        1 ataccaggta tctgggtaaa gaaatctcct atgatgactg cagaaaatta ggagattttg
       61 ttgtcaatgt gatcttatca aatgaaggcc gtgccatgta ttttgacggc tatgattttg
      121 aagagaacga ttatcatgtc atgcatatca gctatgtggc gaaccggatc gtgtatctgg
      181 atcaggaggt tcgcagaact tcttattatc ttacagacga cggatacaat ttgatacttt
      241 ccacccttga aatagaaaat aatatgaagc ttaccatcca tgaaatgatc ttccagatgc
      301 atctggaaaa acagagctat gataaggctg tggatgagat taagaatgtt tttaacctga
      361 tgcgcatcca gcttcagaag attcaggaag ctatgggaaa aatccgcaga aatgccctga
      421 attattcagt aaaggattac gaggaaatcc ttctggagaa tctggatacg atcagtgaca
      481 caaaagaaaa attccagaaa tacagggaac tggtgcgaag cagggtaaag aagctggaag
      541 aagagaacat caatgtgcgc cgtctgggcg aaaaggaaga ggagaacctt gagaatctcc
      601 gtattattga gagctatctg aacagaacca ttgatgagca ccagaaaatc ctgagcagtc
      661 attttgacct gaaggcactt tataccagag aactggaagc attgtcgcag atgtccctga
      721 tccggagatt ttccatgcgc aatgatttct atgataaagt gctggagaat ccgtcagcac
      781 tgggaaatct ggactatttt ttaagaccgc tgttcaaccg ggaaccggac aaggtttata
      841 acctgaacaa agccttatta tatcagaaac cgtctgtcag aaatgaggat gaggatactg
      901 aggagatgct ggattttgac gaagatgcat atctgaaaga gcaggaagag aaacgcagac
      961 agaaactgaa acgctatgag agcagtctgg gatttctgct ggagcaggca tctgaaaaag
     1021 gtgaaatttc cctttctgat atctggaaaa acattcagga gaacgagaaa aatgcagaag
     1081 aaatgcagca gcaactgatc ccaaatgttg aaatttttaa agaaatcatg gtggagctga
     1141 tacggaataa agagatcaat atggaagtac tgaaaaagga acgaagtgaa tttatccagg
     1201 atcagacagc agattttcag ctcaacgaaa tgctgctcca gctttcagaa gaaagatttt
     1261 cagacaaaaa gattggtaaa atagaggtat acaggataga agacggaagc acagtcacct
     1321 ttgacaatgt tttcagcgaa aatggcgtga aaaaaagcat ccgctgttcc aatatcctga
     1381 tccgaatcat gagaaacgag gaataacaga tatggcatac gaagcagagg aaattcgcac
     1441 cagccaggag attttttatt atcttctgga gcatcatgag ctgcgggaag aggacgatca
     1501 gcttttatat aaagcatata cagaggaaga atccatccgg aatctggtga aatcccaggg
     1561 agagattgcg ggaagcaatg tggaacgcta cggaaatgta atctatctga ttcccaaaga
     1621 ggagaataat ttccttggct tttccaagca gcagcttaaa ggtacactgt gcaaatccgg
     1681 ggcaacggac aaggattatt atctgtcaca gtttgtgatt cttacgctac tggtagaatt
     1741 ctttgacgga cagggaagca gcagtaaatc cagagaatat atgcgcgtgg gagagcttat
     1801 gaactgtctt tccgcccgtc tgaaggaagg ggcagcagaa gagactacca ctgaagagga
     1861 ggaagatgga aagaatccca aagatactgc tgggatttct ttcaaaatcc tttatgaaac
     1921 ttacgaggcg ctgaagtctg atgacagagg ttccaggatg aagacaacca aggaaggctt
     1981 tatctacaat attctgatgt tccttcagaa acagggactg atcgagtatg tggtgcagga
     2041 tgagatgata aaaacaacca gaaagctgga taattttatg gactggaatc ttctgaacca
     2101 gaacaattac cagcgggttc tgagagtact gggggtggca aaagatgagt aagattaatg
     2161 cagtgcgtct gatcaacgtg aattataata acaatgcata ccgtatcagc gatgaaactc
     2221 tgcactttaa tggaaaaagc acactgatct ctctgcagaa cggcggtggg aaatctgttc
     2281 ttgtccagat gctgacagcg ccctttgtgc atccaaaata ccgcaatacc aaagaccgat
     2341 tatttgaaag ctattttact accaataagc catcctttat cttggtggaa tgggcactgg
     2401 atcagggtgc aggctatgtt ctcacaggtc ttatggtcag aaaatcccag gatatggaag
     2461 aggacagaaa agagaatctg gatattattg gaattgtcag tgaatatcag agcccctgta
     2521 ttcaggatat ccaccacctt cctgtagtgg aaaaaggcaa aaaagagatg atcctgaaga
     2581 attttaactc ctgcagatag ctttttgaaa cctacaaaaa agacagggat atgaagttct
     2641 tttattatga tctgacaaac tatgctcagt cccgccagta cttcaataag ctgatggaat
     2701 accagatcaa ttataaggaa tgggaaacta ttattaagaa aatcaatctg aaggaatccg
     2761 gactgtcaga tctttttgcg gactgcaagg atgaaaaggg gctgacagaa aaatggtttc
     2821 tcgaggctat tgagagcaaa ctgaataagg acaaaaaccg cattaaagag ttccagtcta
     2881 ttctggaaaa atatgcagga cagtataagg ataaccgttc caaaattaag cgaagagaca
     2941 caatccgtca gtttaaggaa gaagcaaatg ggattcagac tcaggcagaa gagtatcaga
     3001 atgcggagac agaggaagga aaacagcaga ataaaattgc ctggtttatc catgatgtaa
     3061 atggactctt aagccagact gagaaagctc atgatcatgc aaaagaagtg ctggaaggtc
     3121 tggatcaaaa gctttccaga gtagaatacg aggagctttc cagccaggtc tatgactatg
     3181 aggaagagaa gaatcttcat atcggaaacc gtgatatgat cgatatggag cgtgaaaatc
     3241 ttcaggtgca ggcagaacag acagagaaga aacttcatat cctccaatgt gccagacagc
     3301 aggattctgt gtcagaagaa aaaggggaac tggatcttct cagggagaaa atcgcagttg
     3361 ccagacagca gggagctgat ctggaaccga agagaaaagc acttggcctg accttgaaaa
     3421 acatttttga agaacgtatt caggataaca gacaacagca ggaaaacctg tctgaaaata
     3481 tacagaaaaa gggaaaagaa gcacaggatg aggcgaatag agccgaagaa ctggaagaga
     3541 agatccgcga ctgctttgga aaaaaaggaa aactggaaag ccggattgaa tcctataccc
     3601 gtctggaaaa ccagtataat gccaagtatc aggaagaatt agtacgaaat attctgggaa
     3661 cctatgaagc cggaacactg gaaatccgtc gccagatgta cgaacaggaa ctggaaaaaa
     3721 cagtaaggga acgtacagaa aatcagaaga aatgggaaga tgacaaagag ttgatccgcc
     3781 gtcaggagcg cagtctggag gataagaaag aggctctgat acataaaaaa acagagacag
     3841 agaaccagga acatacctat cagctctatc agaatgaact gg
//
LOCUS       DBMDXG010000183         3861 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5493_length_3861_cov_1.192021, whole genome shotgun sequence.
ACCESSION   DBMDXG010000183 DBMDXG010000000
VERSION     DBMDXG010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5493_length_3861_cov_1.192021"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            79..249
                     /locus_tag="ACZPD3_06035"
     CDS             79..249
                     /locus_tag="ACZPD3_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HIR1415592.1"
                     /translation="MSEIKQNITNELSEEELENIAGGFGHPTMDDPRYKEQMRINTML
                     KRGMKMDEIQKR"
     gene            complement(461..652)
                     /locus_tag="ACZPD3_06040"
     CDS             complement(461..652)
                     /locus_tag="ACZPD3_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415593.1"
                     /translation="MYLVGDQQERYIIEEISRSLGKFLGFGLGIIYIMVLVPGTVVLA
                     GTLTNRGSDKVTREKQGQP"
     gene            complement(616..870)
                     /locus_tag="ACZPD3_06045"
     CDS             complement(616..870)
                     /locus_tag="ACZPD3_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415594.1"
                     /translation="MVSVVERSSSGSFDEVIGTVGTDDFGLVLGIALGIVAVALLVNF
                     IVTILIWNPLEVGCKKLFIQCEYGTAETRCIWSVINRRDI"
     gene            complement(1021..1524)
                     /locus_tag="ACZPD3_06050"
     CDS             complement(1021..1524)
                     /locus_tag="ACZPD3_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1415595.1"
                     /translation="MKYEEFECIYQEYCLKILTFIHKRVPDLYEAEELTGDVFLSFYR
                     NMDSYDEEKGSIATWLYAITANRLKNYYRDKKTHYSLEILKQQTIPREKMPEEIVAKI
                     MREETLRKSLEQLSDREREILLGRFYYQKSSTELGRQMNLSPGNVRMIQKRALEKLRM
                     IMEKQGE"
     gene            complement(1514..2452)
                     /locus_tag="ACZPD3_06055"
     CDS             complement(1514..2452)
                     /locus_tag="ACZPD3_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415596.1"
                     /translation="MKKRMIIVTALIMGTLGAGCVSVQASDTLPELTKDNALEQMMKA
                     GETDSLVSNHKSYEATCEYILQNTKVYDYEDSEVMYVDMYGTTQMLYKEGELKYGKEG
                     DEYLSCLYIDEADGNADDLISFYTDETIEEEVTDMKEDGDMIIIQTKVPEELTQSALN
                     SQGDGESYTEGDWISMEYVVASDDYRPLKHTQILNHKDGTSEEASKMEFSYGVDRPKD
                     VADLCEKYEEAEKAEGDVRNVTVTAAPGTEQEKDYAITVPADNHVWIMLHDDNSYYIM
                     YTDKECKELYEPEEDVKEDTHLYAEIESYEEAEDEV"
     gene            2733..>3861
                     /locus_tag="ACZPD3_06060"
     CDS             2733..>3861
                     /locus_tag="ACZPD3_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HIR1415597.1"
                     /translation="MIALRYFCGFSLQIFPYAFFCLYPFRDCVRLSTKKTMLMALSIF
                     IVMVIPFSLIAQFNIGGDYKELIWNVIFYIALLLFGIFYCFSIQAKAAEKLFVFFVVM
                     SYGFFVTSTVTFLHRTFRFPSDYFMYPPFALALTLIINLVLAKPFLMLMERIRTMINA
                     DLESRIWKILCSLPALFILIASIAQFSSIINLSNNIVVHVMFVLFAVFAFMVYAVFFS
                     VMGYIRSKQEEQRISERILENYRNQAENNEHILEIHHEIRHHMNALSSYLKQEDYAGA
                     RQYTQKFTEEAEQLPFVTYTANALVNSILSEFAERASRYKAIVEYSIIISRRLNMEDI
                     DLCRMLTNILENAVEGCQNVSEGQRIIRLNLHSKGNFLSSYI"
BASE COUNT         1043 a          894 c          655 g         1269 t
ORIGIN      
        1 tatagtgtga caaattctct tctgatgttt ataaacagat aggaagcatt cgaattctta
       61 caggaaagga gaactattat gagcgaaatc aaacagaaca ttacaaacga attatctgaa
      121 gaagaattag aaaatattgc cggtggtttt ggccatccta ctatggatga tcccagatat
      181 aaggagcaga tgaggataaa cacgatgtta aaacgaggca tgaaaatgga tgaaattcaa
      241 aaacgttgaa gttctagtgt gccgacaaat tgattcttga agcaatcttg caggataaat
      301 tttttatttc aaggcggagg agagaggcgt agcggtggct acggcgaccg acgccaacgc
      361 ggaaataaga aatttagacg taagattgtt caagaaatca acttgttggc acactagtat
      421 atcgtttcgt aaataactat atcaatcctt ccgggcagcc ttagggctgc ccctgttttt
      481 ctctggtcac cttatcactt ccacgattag tcagagttcc tgccagtacc accgttcctg
      541 gcaccagcac catgatatat atgattccaa gcccaaatcc gaggaacttg ccaaggcttc
      601 ggcttatttc ttctattata tatctctcct gttgatcacc gaccagatac atctcgtctc
      661 cgctgttccg tattcacatt gaataaacag ctttttacat cccacctcca gaggattcca
      721 aatcaaaatc gtcacaataa agttaacaag caacgcaact gccacaattc caagtgcaat
      781 tcctaaaaca aggccaaaat catcagttcc tactgtgcct atcacttcat caaaacttcc
      841 ggaagaagac ctttctacta ccgagaccac tcctgaacct ccaatcaggg ataatataac
      901 tcctcatcaa actgcccata tgcaaatatc tcatatctga aatatgtcat ccggctttca
      961 taatctatta caatgcatta tccccagtca gttcaggcag agtctctgta gcctgaacgc
     1021 ctattccccc tgtttttcca taatcattcg caacttctcc agagcacgtt tctgtatcat
     1081 ccttacattt cccggtgata aattcatctg cctgccaagc tctgtggaac ttttctgata
     1141 ataaaatctt cccagaagaa tttcccgttc tctgtcagaa agctgctcca aactttttct
     1201 taatgtctct tctctcataa tctttgccac aatctcctcc ggcatttttt cccggggaat
     1261 tgtctgctgc ttcaggattt ccagagaata atgagtcttt ttatccctat aatagttttt
     1321 caggcggttt gctgtgattg catacaacca ggtagctata ctaccttttt cttcatcata
     1381 actgtccata tttctgtaaa aagacagaaa tacatctcca gtcagttctt ccgcttcata
     1441 cagatcagga actcttttat gtataaatgt caaaattttc agacaatatt cctgataaat
     1501 acactcaaat tcctcatact tcatcctccg cctcttcata agactcaatc tctgcataga
     1561 gatgtgtatc ttctttcaca tcttcctctg gctcgtacag ttctttacac tccttatctg
     1621 tatacataat gtaataagaa ttatcgtcat gaagcataat ccatacatga ttatctgctg
     1681 gaaccgtgat tgcataatcc ttttcctgct ctgtaccagg agctgcagta accgtcacat
     1741 tacgtacatc accttctgct ttttctgctt cttcgtactt ttcacagaga tctgctacat
     1801 ccttaggtct gtccacgccg taggagaatt ccatcttcga agcttcttct gaagtgccgt
     1861 ccttatgatt cagaatctga gtatgtttca gtggtctgta atcatccgaa gcaaccacat
     1921 attccataga aatccagtct ccctctgtgt aggattctcc atctccctga gaattcaatg
     1981 cgctctgcgt aagttcttca ggtacctttg tctgtataat gatcatatca ccatcttcct
     2041 tcatatccgt cacttcttcc tctatggttt catctgtgta aaagcttata agatcatctg
     2101 catttccatc tgcttcatca atatacagac aggagagata ctcatctcct tcttttccgt
     2161 attttaattc gccctcttta tagagcatct gggtagtacc atacatatct acatacatca
     2221 cttcagaatc ttcatagtca tatacctttg tattctgcag tatatattca caggtagctt
     2281 catagctttt atgattgctt acaagactgt ctgtttctcc tgctttcatc atctgttcca
     2341 gtgcattgtc ttttgtcagt tcaggtaacg tatccgatgc ctgaacactc acacatcctg
     2401 ctcccagagt tcccataatc agcgccgtca caataatcat tcttttcttc attttttagt
     2461 ctccttttct tatatttttt cctttacgat gccagtaaaa gcgaactttc gggcatatat
     2521 accgtatcaa aagctctcct cattttgaca gttttctgta tctgtattat ttatgttatt
     2581 tgaattttac ttgcctgtct atatttttgc gtgaatagtc aaaatatggc gtaaaaagac
     2641 ctctgtctgg tttgatttta ccatctgcca atggtatact tatttttaat aagtaacctg
     2701 gccattatct atctttatga cgggagggtt ttatgatagc attgcgttat ttctgcggat
     2761 tttctcttca gatttttcct tatgcatttt tctgtttgta tccattccgg gattgtgtcc
     2821 gcctaagcac aaaaaagaca atgctcatgg cactgtccat ttttattgta atggtcattc
     2881 cgttttctct tatcgcacag ttcaacatcg gcggtgatta caaagagctg atctggaatg
     2941 ttatctttta tattgccctg ctactgttcg gaatatttta ctgctttagc atccaggcga
     3001 aagcagcaga aaaactgttt gtattttttg ttgtaatgag ctacggattc tttgttacaa
     3061 gcactgttac ctttttgcac cgtactttcc ggttcccatc cgattatttt atgtatccgc
     3121 cctttgcact ggctctcaca cttatcataa atctggttct tgcgaaacca tttctcatgc
     3181 tgatggagcg aatccgtact atgatcaatg cagatctgga atcacggatc tggaagattc
     3241 tctgctccct ccctgctttg tttattctga ttgccagcat tgcacagttc tcaagcataa
     3301 taaatctttc caataatata gttgtccatg ttatgtttgt actgtttgca gtatttgctt
     3361 tcatggtgta tgccgtattc ttcagtgtca tgggatacat ccgcagtaaa caggaggaac
     3421 agcggatttc tgagcgcata ctggaaaact accggaatca ggcagaaaac aacgaacata
     3481 ttctggaaat acaccatgag atccgacatc atatgaatgc cctgtcctcc tatcttaaac
     3541 aggaggacta tgcaggtgcc agacagtata cacagaaatt tactgaagaa gccgaacagc
     3601 ttccttttgt cacctacaca gccaatgctc tggtcaattc catcctttcg gaatttgcag
     3661 aacgtgccag ccgttataaa gccatcgtgg agtacagtat tatcattagc agaagactca
     3721 acatggaaga cattgatttg tgccggatgt tgacaaatat attggaaaat gcagtagaag
     3781 ggtgtcagaa tgtttctgaa ggacagcgga tcatccggct gaatctccat tcaaagggaa
     3841 attttctgtc aagctacatt t
//
LOCUS       DBMDXG010000184         3853 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5501_length_3853_cov_1.460021, whole genome shotgun sequence.
ACCESSION   DBMDXG010000184 DBMDXG010000000
VERSION     DBMDXG010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5501_length_3853_cov_1.460021"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="ispE"
                     /locus_tag="ACZPD3_06065"
     CDS             complement(<1..659)
                     /gene="ispE"
                     /locus_tag="ACZPD3_06065"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR1415598.1"
                     /translation="MRLRALAKINLGLDVVGKRDDGYHEVRMIMQTIQMYDVLEIEKK
                     KEPGIVLTTNIPYVPTDERNLVYKAAKMLMDEFDIKEGLTMNLEKFIPVAAGMAGGSS
                     DAAAAFVGVNRLFGLGLSEEELMKRTVKVGADVPYCVMRGTALAEGIGEKLTRLPRVP
                     HCYVLVGKPGVNVSTKTAYENLKLDDPAVVHPDIDGMVTAVRNGDLDGMISRMGNVFE
                     PG"
     gene            complement(900..2456)
                     /locus_tag="ACZPD3_06070"
     CDS             complement(900..2456)
                     /locus_tag="ACZPD3_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR1415599.1"
                     /translation="MELRKESVQMLQIKSRAASQVTFDTDYNVPDAKPDVGRLIQNKG
                     DVSMDEVRLSEGKAFISGNLNVDILYVGEENQKVSSLGAKLPFDETLNLEGIASGDKM
                     CLKWEIEDLSVHMIHSRKLNIKAIVTFYAVVDEMAGIQLPVEISEEGISVKRKKVRLM
                     SLMVHKKDTMRIKDEVTLVSNKPNIEQLLWYTIDVRGMDLRPEDNVVKARGELSVFVL
                     YSGEDEENPLQWAEYVLPFNTEVECTGCMGEMIPNIGFSVMHQSIEVKPDSDGEERVM
                     SVDTVLELDMKLYREEEHDLILDVYSPLKECIPQGKEMCLESLLVRNDSKCRVSDRIE
                     LKESQGKILQICHSQGRVKVEKTKIVENGIQADGIVFMKILYITGNDEMPFYSVDGMI
                     PFSHIIEANGINEDSIFFLQADLEQLSTSMIDSNEIEVKAVISLNVLVLQCENRMIIS
                     KVEERPLDMEKIQAMPGITVYVVKNGDSMWDIAKRFYTTVEEICELNELEEDRVVPGM
                     PLLLVKKVEG"
     gene            complement(2486..2932)
                     /locus_tag="ACZPD3_06075"
     CDS             complement(2486..2932)
                     /locus_tag="ACZPD3_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR1415600.1"
                     /translation="MKKTKAVLLGVILWSLLLISGCNFIRIEEEPRKPLEYSIVKNEE
                     IPAELAALIQEKKKNEFQLTYQCEKDLFLVKGYGEQMSGGYSISVEELGWTSQAVFFR
                     TMLLGPSDEKITISPPSYPYIVVKTEYRKEPVIFERFTEKSENDAI"
     gene            3135..>3853
                     /locus_tag="ACZPD3_06080"
     CDS             3135..>3853
                     /locus_tag="ACZPD3_06080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR1415601.1"
                     /translation="MIRFILCVIVVVGFLILSIPILFVEWIIGKFNPMAKEISSLRIV
                     QAVFRFILWISGVKLTVIGEENVPTDVPVLYIGNHRSFFDVPITYPRCPIRTGYIAKK
                     EMEKVPLLSTWMKRLHCLFLDRNDLKQGLKTILTAIEKVKSGISICIFPEGTRNKVPD
                     TFLPFHAGSFKIAEKSGCPIVPIALNNAGDVFEDHLPKIKKTHVVIEYGEPIYPNELP
                     KEDKKRLADITLERIKEMYFK"
BASE COUNT         1016 a         1012 c          663 g         1162 t
ORIGIN      
        1 cccggttcaa atacatttcc cattctggaa atcattccat caagatcacc atttctgaca
       61 gctgtcacca taccatcgat atccggatga accacagcag gatcgtccag tttcaggttc
      121 tcataagcag tcttggttga aacatttact cctggttttc ccaccagaac atagcagtga
      181 ggtactctgg gaagtctggt cagcttctcc ccgattccct ctgcaagagc agttccgcgc
      241 attacacaat aaggaacatc agctccaacc ttcacagtac gcttcatcag ctcttcctca
      301 gaaagtccca gtccaaacag gcggttcact ccaacaaaag cagctgccgc atcagagctt
      361 ccaccagcca tgccggctgc taccgggata aacttctcca gattcatggt aagcccctct
      421 tttatatcaa actcatccat taacattttc gccgctttat atacaagatt ccgctcgtca
      481 gtaggaacgt aaggaatgtt cgtggtaagg acaattcccg gctccttttt tttctcaatt
      541 tcaagcacgt catacatctg tatggtctgc atgatcatac ggacttcgtg atatccgtca
      601 tcacgttttc caaccacgtc aagtcccaga tttatctttg caagtgcacg taaacgcata
      661 atttcctccg cttgtagctg ccgctcacat aaaacctctg gcaggctcta agagcagcag
      721 tttctttgta ctttgtatac aattttatca atgtttttat tgtaaaatat ttttgtattt
      781 ttttcaatac atttatgcga ggaaatttct cattctctat atatcgttac tgtccacaac
      841 gtgaccagag catgtttgaa aaggtttctc caaatatatg cagtaaacaa aaagcagcct
      901 tacccttcca ccttcttcac caacagcagc ggcattccag gcacaactct gtcctcttcc
      961 agttcattca gctcacagat ttcctccact gtagtataaa atcttttcgc aatatcccac
     1021 atagaatccc catttttcac cacatacacc gtgattcccg gcattgcctg gattttctcc
     1081 atatcaaggg gacgctcctc cactttgctt atgatcatcc ggttttcaca ctgaagcacc
     1141 agcacattga gactgatcac agccttcacc tcaatctcat tgctgtcgat catagacgtt
     1201 gacagctgtt ccagatctgc ctgcagaaaa aagatactgt cctcgttgat cccgtttgcc
     1261 tcaattatat gggaaaaagg gatcattcca tcaacagaat agaatggcat ttcatcattt
     1321 cctgtaatat acagaatttt cataaataca attccatcag cctggattcc attttctaca
     1381 attttcgttt tctcaacctt tacacgtccc tggctgtgac agatctgcag gattttcccc
     1441 tggctttcct tcagttcaat acggtcagat acgcggcatt tggagtcatt ccgtaccagg
     1501 aggctttcca gacacatctc ttttccctga ggaatgcatt ctttcaaagg cgagtacaca
     1561 tccagaatca ggtcatgctc ttcttcgcga taaagcttca tgtccagttc cagcaccgtg
     1621 tctacactca ttacccgttc ttccccatcg gaatccggtt ttacttcaat gctctgatgc
     1681 ataacggaaa agccaatatt tggaatcatc tctcccatac aaccagtaca ctccacttcc
     1741 gtattaaacg gaagcacata ctctgcccac tgaagcggat tttcctcatc ttctcccgaa
     1801 tacaggacaa aaactgacag ctcccctctt gccttcacca cattatcctc tggacgcaga
     1861 tccattccac ggacatcgat ggtataccac aaaagctgtt caatgttcgg tttattggac
     1921 actaaagtca cttcatcctt aatccgcata gtgtcctttt tatgtaccat aagactcatt
     1981 aaacggactt tcttcctttt tactgaaatt ccctcctcac tgatttccac cggaagctga
     2041 atccctgcca tttcatctac cactgcataa aaggtgacaa ttgcttttat gtttagtttt
     2101 cgggaatgaa tcatgtgcac gctgagatcc tcaatctccc acttcaggca cattttatcc
     2161 ccacttgcaa tcccttccag gttcaaagtc tcatcaaaag gcagttttgc ccccagactg
     2221 ctcaccttct ggttttcttc ccccacatag agaatatcca cgttcaggtt tccgctgata
     2281 aatgcctttc cttcgctaag acgcacttca tccatggaaa cgtcaccctt gttctggatc
     2341 agtcgaccca catccggctt tgcatctggt acattataat ctgtatcaaa cgttacctgg
     2401 ctggctgcac ggctttttat ctgaagcatc tgcacacttt cttttcttaa ttccacgatt
     2461 ttccactcct tttttcacca gactgctata ttgcatcatt ttcgcttttt tcagtaaagc
     2521 gttcaaaaat cacaggttct tttcggtatt ccgtcttcac cacgatgtaa ggataggaag
     2581 gcggactgat cgttattttc tcgtctgatg gccccagcag cattgtccgg aaaaaaacag
     2641 cctgggaagt ccaccccagt tcttccacgg aaatactgta acctccgctc atttgctccc
     2701 cgtatccttt tacaagaaac aagtcttttt cacattgata ggtcagctga aattcattct
     2761 tctttttctc ctgtatcagg gcagccagtt ctgccggtat ctcttcattt tttacaatgg
     2821 aatactccag aggcttccgc ggctcttcct caatgcggat aaaattgcat ccactgatca
     2881 aaagcagtga ccacaaaatt actcccagaa gaactgcttt tgtttttttc attctgccac
     2941 ctcagatgtc tcgttgctgt tcatctgatg ctcagccttt aaacacagca aacagcaatc
     3001 tccatttatt ttattgtatg aggaataaaa aagatttatt gcaagaattt ttcaaattac
     3061 atataataga cataaacaat atgccaaaac atgcttccta cgcatgaggc acagatcaaa
     3121 gaaaggaaaa tcatatgatt cgttttattt tatgcgtgat cgtagtggtt ggattcctga
     3181 ttttatccat tcccattctc tttgtagaat ggatcatcgg caaattcaat cctatggcaa
     3241 aggaaatcag ctctcttcgg atcgttcagg cagtatttcg ttttatcctg tggatatccg
     3301 gtgtgaagct gactgttatc ggagaggaaa atgtaccaac tgatgtgccg gttctttata
     3361 tcggaaatca cagaagcttt tttgatgtac cgatcactta tccacgctgc cccatccgta
     3421 ccggctatat cgcaaaaaaa gagatggaaa aggttcctct gctttctacc tggatgaaac
     3481 gtcttcactg cctgtttctt gaccgtaatg acctgaagca gggactgaaa accattctca
     3541 ctgctattga gaaagtgaaa tccggcattt ccatctgcat tttcccggaa ggcacccgca
     3601 acaaggttcc cgacactttc ctgcctttcc atgcaggaag cttcaaaatt gccgagaaat
     3661 ccggctgtcc catcgtccct attgctttga ataacgccgg tgatgttttt gaggatcatc
     3721 ttcccaagat aaagaaaacc catgtcgtca tcgaatacgg cgagccgatt tatcccaatg
     3781 agcttccaaa ggaagacaaa aagaggctgg cggatattac gctggagaga ataaaagaga
     3841 tgtattttaa gaa
//
LOCUS       DBMDXG010000185         3850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5504_length_3850_cov_1.459589, whole genome shotgun sequence.
ACCESSION   DBMDXG010000185 DBMDXG010000000
VERSION     DBMDXG010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5504_length_3850_cov_1.459589"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="ACZPD3_06085"
     CDS             complement(<1..2399)
                     /locus_tag="ACZPD3_06085"
                     /EC_number="3.2.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004567 - beta-mannosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005537 - D-mannose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HIR1415602.1"
                     /translation="MIKLQLHENWQLCNIRQLDWIPAQIPGDIYAALLKTGKMPDPFF
                     GDNEYQAKALMEEDYEYRTVFNYEEAQFKDCQEVILRFDGIDTIADIYLNGCCLGKVD
                     NMHRIWEFPVGELLENGKNTLRVIIRSPLKFMAEAFKKYKNRGNEDTIEGFMHLRKAH
                     YMCGWDWGACLPDGGIFRPVTLLGIETARLDSVYIRQVHKDGKVLLVPEVDVETVDEE
                     ESEADGYEGAQALEYQVTVTAPNGTKTIWDDCPDEMEIENPQLWWPNGLGEQPLYQVQ
                     VDLKAGNKIVDTWCRKIGLRALTMHREKDQWGESFAHEVNGYQVFAMGADYIPEDNLL
                     QRTSRERTRELLLQCKRANFNTVRVWGGGYYPEDWFFDLCDELGLMVWQDFMFACSVY
                     ELTPEFEANIRQEFIDNIKRLRHHASLALWCGNNEMEMFVKQGTWVTKPTEMRDYLFM
                     YERIIPEVLSEYDPDTFYWPASPSSGGSFDEPNDPNRGDVHYWEVWHGNKPFSEYRKY
                     FFRYASEFGFQSFPSVKTLETVTDDPKELNPFSYVMEKHQRNYGGNGKIAKYMQAAYR
                     YPENFSDFVYASQLLQADGIRYGVEHYRRNRGRCMGAIYWQLNDCWPVISWSSIDYYG
                     RWKALHYYAKRFFAPVMLSCEEQSWMTVEADMNRQHFEFEKSIRLNVTNETLDAHRVL
                     VKWAVRKTDATILREEEQWLEVPVLSAVWMDKVELPQIDCFNEYVSYEAWENGQIISQ
                     GTVIFSYPKYFHYLNPGLAVRVDGDEIVVKAEAYAKSVEIRNENDDLILEDNYFDMNA
                     G"
     gene            complement(2576..3712)
                     /locus_tag="ACZPD3_06090"
                     /pseudo
     CDS             complement(2576..3712)
                     /locus_tag="ACZPD3_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         1001 a          981 c          668 g         1200 t
ORIGIN      
        1 tctcctgcat tcatgtcaaa ataattatct tcaagaatca ggtcatcatt ctcattccgg
       61 atttccacac tctttgcata tgcttctgcc ttaacaacta tctcatctcc gtctacgcgc
      121 acagccagtc cgggattcag atagtggaag tatttcggat aggaaaaaat cacagttccc
      181 tgagaaataa tctgaccatt ttcccatgcc tcataactta catattcatt aaaacagtca
      241 atctggggaa gttcgacctt atccatccat acagcagaca ataccggtac ttccagccac
      301 tgttcttcct ccctcagaat tgtggcatct gtctttcgca ctgcccattt caccagaacc
      361 ctgtgagcat ccagcgtttc attggtcaca ttcagacgga tggatttttc aaattcaaaa
      421 tgctgacggt tcatatccgc ttccactgtc atccagctct gttcttcaca ggaaagcatt
      481 acaggcgcaa agaatctctt cgcataataa tgaagcgctt tccagcgtcc gtaataatca
      541 atggatgacc aggaaataac cggccaacag tcatttaact gccagtagat tgcacccata
      601 caacgtcccc ggtttctgcg gtaatgctcg actccatatc ggattccatc tgcctggaga
      661 agctgagatg cataaacaaa atcgctgaag ttctcaggat aacggtatgc tgcctgcatg
      721 tatttggcaa tttttccatt tcctccgtaa tttctctgat gtttctccat cacataagaa
      781 aatggattca attctttcgg atcatctgtt actgtttcaa gcgtttttac agacgggaat
      841 gactgaaaac caaactcaga tgcatatctg aagaaatatt tacgatattc tgaaaatggc
      901 ttattgccat gccatacttc ccagtaatgc acatctccac ggttcggatc attgggttca
      961 tcaaaggaac cgcctgatga cgggcttgca ggccagtaga aggtatccgg gtcgtattcg
     1021 ctgagaactt ccggaataat gcgctcatac ataaacagat aatctctcat ttctgtcggc
     1081 tttgtcaccc aggtgccctg ctttacaaac atttccatct cattgtttcc gcaccacagg
     1141 gcaagggatg catgatgacg gagacgtttg atattatcaa taaattcctg acggatattt
     1201 gcctcaaact ccggtgtcaa ttcatagaca gagcatgcaa acataaagtc ctgccatacc
     1261 atcagcccca gttcatcaca gagatcaaag aaccagtcct caggataata gccgccgccc
     1321 cagacacgta ctgtattaaa gttcgctctc ttgcactgaa gaagtaattc tctggtacgt
     1381 tctctggatg ttctctgcag aagattgtcc tcagggatat agtcagcgcc catggcaaag
     1441 acctgataac cgtttacttc atgagcaaag ctctcgcccc actggtcttt ctctctatgc
     1501 atggtcagcg cacggagacc gatttttctg caccaggtgt ctacaatctt atttccggct
     1561 ttcaggtcta cctgcacctg atataacggc tgttctccca gtccatttgg ccaccagagc
     1621 tgcggatttt caatctccat ttcatccgga cagtcatccc atatggtttt tgttccattc
     1681 ggtgcagtta cagtcacctg atattccagt gcctgtgcac cttcataccc atctgcttcg
     1741 ctctcttcct catcaacagt ctccacatct acttccggaa ccagcaggac tttgccatcc
     1801 ttatgtacct gacggatata cacgctgtcc agtctggcag tttcaattcc cagaagtgtt
     1861 acaggacgga agatacctcc atccggcaga catgcacccc agtcccagcc acacatatag
     1921 tgagctttgc gcagatgcat aaatccctca attgtgtctt catttcccct gtttttatat
     1981 ttcttaaatg cctctgccat gaacttcaac ggagagcgaa tgataacgcg taaggtattt
     2041 ttgccgtttt caagcagctc tcctaccggg aattcccaga ttctgtgcat gttatcaact
     2101 tttccaagac aacatccatt cagatagatg tccgcaatcg tatcaatccc gtcaaatctc
     2161 aggatcactt cctgacaatc tttaaactgc gcttcttcat aattaaatac cgttctgtat
     2221 tcgtaatcct cctccatcag tgctttcgcc tgatattcat tatccccaaa gaacggatcc
     2281 ggcatctttc cggtcttaag aagtgccgca taaatatccc ccggaatctg tgccggaatc
     2341 cagtccagtt gtctgatatt gcacaactgc cagttttcat gaagctgtaa ttttatcatt
     2401 tgttttatcc ctctttgatt attttttaca tatgtttgca ttcaactata tatatagtat
     2461 catactgccc ttttctaaat caactctgca aaagaaatca ttactgttca aaggtatgta
     2521 tgttactgtt cactctgttc ctagtaatac gttttgtaat cacataaaag tcagttacag
     2581 gcagttttcc cttttcaccc ggaaggctcc tgattgctgc agttgcctca taaccattca
     2641 tgacaggcat ttggatatcc ataaaaataa gatcatacga acctttcgga gaagcttcca
     2701 ctttttcaac tgcgattttt ccattttctg ctgtttccac ttctgccctt gtcatctgca
     2761 aaatttcaac accaatttct ctgtttaact cattatcctc aactaacaga atcctttttc
     2821 ccgtgtagtc tgattctgac agctcctcca gataatttct tgctgttttt tcttttcttc
     2881 ctgaagtaaa ctgccgcagt gtagccgtca gccgggaacg gaacagcggt tttgcaataa
     2941 aagcatctac acctgcagcc tttgcttcct cttcaatttc gctgaattca taggatgtca
     3001 atacaatgat ggtgatctct ttcccgatcc gtttccggat ctgtctggca gtttctatac
     3061 gatctatctc cggcatcttc cagtccagga taacagcaaa ataatcattt tttagctcat
     3121 gacgtgcata acaacattca acagcttccc tgccagagag aacccattca cctgtaatac
     3181 cgatttcttt cagtgtggca attgtacttt cacagcatgt cttatcatca tccacaacca
     3241 gaaccggcag attcatcaga ttttcatcct gttctttttc tttttcctga agttccagat
     3301 aaattgttac cgtaattctg gttcccttgt gtaaggtact gtccacttta atattaccat
     3361 tcatcaggtt cacaatattt ctgctgattg ccattccaag accggttccc tgaactctgg
     3421 ttgtccggtg gtcatccgcg cggctgaaag gatcaaacat gattttctgg aattccggtg
     3481 acataccgat cccattatcc tcaatcgtaa attcatagca tccaagttcc gaaaatccat
     3541 ttggcttttc ctctatggaa aagataatat tcccgccatc cggtgtatac ttaattgcat
     3601 tgctcatcag attcacaaat acctgctgaa tcctcaggct gtcaccacag acatcctcgt
     3661 gttcaatgtg attgatgtgt atatcaaaat tgtgtttatg ttcatccagc accataaaaa
     3721 ttccttgatt ttataagggt tttatctctt ggttttttta agtaagcagg aagtaagcaa
     3781 gcaagtgtaa tcaaggcatg ataaagagtt tatccacaaa gtgatacttt acggataagc
     3841 tcttttttat
//
LOCUS       DBMDXG010000186         3849 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5505_length_3849_cov_2.134205, whole genome shotgun sequence.
ACCESSION   DBMDXG010000186 DBMDXG010000000
VERSION     DBMDXG010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_3849_cov_2.134205"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="ACZPD3_06095"
     CDS             <1..698
                     /locus_tag="ACZPD3_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1415603.1"
                     /translation="ITDKVVAANIPLVYVNREPSEDEEKRWEDNGWNVTYVGCDARQS
                     GTMQGEIIADLGLDTIDKNGNGKVDYIMIEGDPENVDAKYRTEFSVKALEDAGLEVNQ
                     LDDQVGNWDQATAQQLVANDLSQFGDDVEVVFCNNDAMALGALQSIEAAGRTVGDDIF
                     LVGVDALSEALDKVEEGKMTGTVFNDHISQSHSAADAAVKYLAGEENEHYIGCDYVKV
                     TTDNVADIKEMTK"
     gene            783..2290
                     /locus_tag="ACZPD3_06100"
                     /pseudo
     CDS             783..2290
                     /locus_tag="ACZPD3_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            2310..3296
                     /locus_tag="ACZPD3_06105"
     CDS             2310..3296
                     /locus_tag="ACZPD3_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HIR1415604.1"
                     /translation="MGKKKLSFKEIMTNYGVIIIMVCLIVFTGITRPNFITTNNLTNI
                     LATMSFRLVIALGIAGCIITAGCDLSAGRMIGFGGCIAGTLLQKMDYSAKFYPGMKPL
                     NIWLALLIVMLICAAFGLVTGFFIAYLNVPPFISTMAMMEIVYGICSIYTNATPLGGY
                     VSEYTGLATGKFLGMSYLVWIAILVGVITWFIFNMTRHGKYMYAIGGNPQAAEVAGVP
                     VKKTLIIIYAKAAAMYGLAGFMMGAKAGGASVQLGYGYEMEAIAACTIGGVSVTGGRG
                     KVSSALVGVAVFEFLKAALQFLGVNAYAQYIALGVIIFIAISLDIRKYIARK"
     gene            3394..3726
                     /locus_tag="ACZPD3_06110"
     CDS             3394..3726
                     /locus_tag="ACZPD3_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415605.1"
                     /translation="MGNSYLSNVMIPLMMTLVLGYYAARLLIFHDTESITGKDNGKKL
                     KDKEKYVAESGKLILFLAAGSFVMAILMYFSVHAAVVEIAVWFVVFGILWKRMNDKYG
                     APNSHKKK"
BASE COUNT         1197 a          658 c          938 g         1056 t
ORIGIN      
        1 gcattacaga taaagtagta gcagctaata ttccgcttgt atatgtaaac cgtgagccaa
       61 gcgaagatga agagaagaga tgggaagaca atggctggaa cgttacatat gtaggatgcg
      121 acgctcgtca gtccggaaca atgcagggtg agatcattgc tgatcttggt cttgacacaa
      181 ttgataagaa cggaaacgga aaagttgatt atatcatgat cgaaggtgac ccggagaacg
      241 ttgatgctaa atatcgtaca gaattctctg taaaagctct tgaggatgca ggacttgaag
      301 ttaaccagct tgacgatcag gtaggtaact gggatcaggc tacagctcag cagctcgttg
      361 ctaacgactt aagccagttt ggtgatgatg tagaagttgt attctgtaac aacgatgcta
      421 tggcacttgg tgctcttcag tctatcgaag ctgccggacg tacagttggt gatgacatct
      481 tccttgtagg tgtagatgct ctgtccgagg ctcttgataa agtagaagaa ggcaaaatga
      541 caggtacagt atttaacgat catatttctc agtctcattc agcagcagat gcagctgtta
      601 aatatcttgc aggcgaagaa aatgaacact acatcggctg tgattacgta aaagtaacta
      661 cagacaacgt agctgatatt aaagaaatga caaaataatt taattgatct tgctcagaaa
      721 ggtgcgggtg acttaaaagg cacccgtact tttctgtgag aaaagggaaa gggaggagaa
      781 atatgtctga gtacaaactg cagctgaagg gcgtctgcaa atctttccca ggtgttaagg
      841 cacttgataa tgtacagctg tcactccgtc cgggtacagt acatgctctc atgggagaga
      901 atggtgcagg aaagtctact ttgatgaaat gtttattcgg tatttacaaa atggatgccg
      961 gagaagtcta cttagatgga cagaaaattg tagttaataa tcctgatgaa gcaatgaaat
     1021 atggtatcgc aatggtacat caggagttac agccggttcc ggcaagaaca gtagcggaaa
     1081 atctttacct tggacgtttt cctacaaaga aatacggtcc tttacaggta attgaccata
     1141 aaaagatgta tgaagataca gcatactggc tgaaagaagt taagatggat tttgatccga
     1201 aggctcttct gggtgatctt tctatcggac agatgcagtc agtagaaatt gcaaaagcag
     1261 tttcacacaa tgcgaaggtg gtaatctttg atgagcctac atcctcatta tcagataacg
     1321 aagtagaagc tcttttcagg atcatgaatg atctgagaga taagggtgtt gccatggttt
     1381 acatttctca taagatggat gagatcaaac gtattgcaga tgatatcacc atcatgcgag
     1441 acggtacata tgtaggaacc tggcctgcag ctgaaatgac tacagaagat atcattacca
     1501 agatggtagg acgtgagctg accaatgtat atccggaaag aaagaataaa ccgggagaag
     1561 tgatcatgga ggtaaaacat ttaagttcta tccatgagaa atcattccag gatgtatctt
     1621 tcacactccg caaaggtgag atcctcggat ttggcggtct ggtaggagct cagagaacag
     1681 agcttctgga aggcgttttc ggaatgagag cgatccagtc cggagagatt tatattaaag
     1741 gtaaaaaagt aaatatcaaa catccggcag atgccatgaa agccggaatc ggtctgatca
     1801 agaggacaga cgtggaaatg gtatttttgg atgtctttcc atcaaggata atgtaggtgt
     1861 ttctatttat aacaaacatt tgaaagccgg atttgtgctt gatcataaga cgatcaacgg
     1921 aatcgtagac gacagtatca agaagctgcg tatcaagact ccgaatatgc aggagcatat
     1981 cgcaaacctg tccggaggta accagcagaa ggttatcgtt gcaagatggc tggcaaatga
     2041 tccggatatc ctgatcatgg atgagcctac ccgtggtatc gacgttggtg caaaacacga
     2101 gatctatgag atcatgaacg acctggcagc agaaggcaaa gccattatca tgatttcttc
     2161 agaaatggca gagctgcttg gtatgtctga ccgtgtttat gtaatgtgta acggaaagat
     2221 gcgcggagaa cttacagagg aaaatgaaat gactcaggaa catgttatga gttatgctac
     2281 aaaattctga tgatgggagg agaaaaataa tgggtaaaaa gaaattaagc tttaaagaga
     2341 ttatgacaaa ttatggtgtt atcatcatca tggtttgcct tatcgtattc acaggaatca
     2401 caagaccaaa ctttataaca acaaacaacc ttactaatat tcttgcaact atgagtttcc
     2461 gtctggtaat cgcacttggt attgccggat gtatcattac agcaggatgt gacctttccg
     2521 caggtcgtat gattggtttc ggcggatgta ttgcaggtac actgctccag aaaatggatt
     2581 attccgctaa attctatccg ggtatgaaac cgttaaacat ctggcttgca cttctgatcg
     2641 ttatgctgat ctgtgcagca ttcggtctgg taacaggttt ctttatcgca tacctgaacg
     2701 taccgccatt catttccaca atggctatga tggaaatcgt ttatggtatc tgcagtattt
     2761 atacaaatgc aactcctctt ggaggatatg taagtgagta tacaggactt gctacaggta
     2821 aattccttgg aatgagctat ctggtatgga tcgcaatcct ggttggtgtt attacatggt
     2881 ttatctttaa catgacccgt catggaaaat atatgtatgc aatcggtggt aacccacagg
     2941 ctgcagaagt tgccggtgtt ccggttaaga aaactcttat cattatctat gcaaaagcag
     3001 cagctatgta tggtcttgca ggatttatga tgggtgcaaa agccggcggt gcatctgttc
     3061 agttaggtta tggttatgaa atggaagcta tcgcagcttg tactatcggt ggtgtatctg
     3121 taaccggtgg tcgtggtaaa gtatcttccg ctctggtcgg tgttgcagta ttcgaattcc
     3181 tgaaagcagc acttcagttc ctgggagtaa atgcatacgc tcagtacatc gcacttggtg
     3241 ttatcatctt cattgctatt tcactggata tcagaaaata cattgcaaga aaataataaa
     3301 aagtatataa tgttacatac agggccgccg ttttggtacg acggtccttt ctttatatat
     3361 acagctgcga aataaaaaga ggagaaataa aaaatgggaa acagttattt atcaaacgta
     3421 atgattccgc tgatgatgac tcttgttctt ggatattatg cagcccgtct gctgatattt
     3481 catgatacag aatccatcac agggaaagat aatggaaaga aacttaagga caaagagaaa
     3541 tatgtggcag aatcaggaaa gctgatactt tttcttgcgg caggttcttt tgttatggca
     3601 atcttaatgt acttcagtgt ccatgcggca gttgtggaaa ttgcggtgtg gtttgtagta
     3661 ttcggtattt tatggaaacg tatgaatgat aaatacggag ctccgaattc acataagaag
     3721 aaataagata accatttagt agagtgctga ttgtgaaggc gctgaacagt tacaggacaa
     3781 aaaacagaaa tttagagaca ggtgtagaga tggagaagta ttcggtactg cttgtggatg
     3841 atgaggaag
//
LOCUS       DBMDXG010000187         3847 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5508_length_3847_cov_1.703684, whole genome shotgun sequence.
ACCESSION   DBMDXG010000187 DBMDXG010000000
VERSION     DBMDXG010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5508_length_3847_cov_1.703684"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="ACZPD3_06115"
     CDS             <1..485
                     /locus_tag="ACZPD3_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415606.1"
                     /translation="ADVSERIQYLVTQVEEITKIDNFGAYLTAMLEIDAFFMNEDRHT
                     NNIAVIYNEKTQKYELSPFFDQGLCMFADINQDYPIDQSLEYCLEKIEAKPFSSDFDI
                     QLDAAEELYGIQIGFQFTFKDIQIYLQKNSENYPREVIRRVEQLLRGQMRKYGYLMKK
                     "
     gene            complement(593..766)
                     /locus_tag="ACZPD3_06120"
     CDS             complement(593..766)
                     /locus_tag="ACZPD3_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase"
                     /protein_id="HIR1415607.1"
                     /translation="MLVCDYIVESIDGDYAHLRRTDLPEEELKLVARALLPFEITEGC
                     RLHYEMMQYSIID"
     gene            924..1139
                     /locus_tag="ACZPD3_06125"
     CDS             924..1139
                     /locus_tag="ACZPD3_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415608.1"
                     /translation="MSKVVKLSSVMEREEKLKEIYEGLEEIKDQLTALIDEYEEEETH
                     TGKADELIEALDALEDASETIEDVLEN"
     gene            1139..2038
                     /locus_tag="ACZPD3_06130"
     CDS             1139..2038
                     /locus_tag="ACZPD3_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415609.1"
                     /translation="MKEKAYFHLPGLFEFYELYRMFLPLFREHREYFYDWCEIGSIYG
                     APADCIWGGGRAGFGENDPEEVLELMQEYGISARLTFSNSLLRQEHLSDRKCNALCRL
                     FERNREPQNGVIIYSELLLEYIKEQYPGLYFVSSTTKVLTDFTQLEEEIRRKDFHYVV
                     PDFRLNKAFDKLNTLSQAEKDKVEFLCNECCWFGCRDRKACYETVSRKNLGENCLEHY
                     CKAPERERGYLFSKAIENPGFIGINDIRDIYLPMGFSNFKIEGRGLGSALILEFLLYY
                     MTRPEYQIHVREKIYLDSMLDLF"
     gene            2240..2569
                     /locus_tag="ACZPD3_06135"
     CDS             2240..2569
                     /locus_tag="ACZPD3_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR1415610.1"
                     /translation="MAIEKSLVSGSMTMLILKLLSEKDMYGYEMIDTLRQKSQNVFEL
                     KAGTLYPLLHSLEDKGFLTVYEQEAAGKTRKYYSLTGQGRGFLEKKVEEWKEYSAAVT
                     NVLVMEV"
     gene            2573..>3847
                     /locus_tag="ACZPD3_06140"
     CDS             2573..>3847
                     /locus_tag="ACZPD3_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR1415611.1"
                     /translation="MDEYLKTLLEQIRCKKARPYVKQEFQDHIEDQIEANMQAGMDRE
                     QAEREAVRDMGDPVETGISLDSVHRPQVAWKLLGIIVLISIAGVLIHAGIAGKISENA
                     AAGSDRYVFHVVIGLAVMMILYLLDYTVLARFSKIIAVILLFACLVTLLGGYQLNGAR
                     YFIVLPGGRGISMQTLMLFYVPIYGAILYKYHGWGYKGLMRAIIWMIAPVILVYAMPA
                     LMPACMMLVSMLVMLTIAIQKNWFTVRKKKAICGIWAGFLAMPVAAFLIRYLSSSLTE
                     YQIARLQAIFSDGGEEDYFTGMLHSFWQQNKWIGKSESDVMGNLPAFNADYILTYLSS
                     VYGTIAAVLLCCVLAVLIFAVFNTSMRQKNQLGMMMGCGCGIVFLVNFSINILENLGV
                     FPQSVTFLPFLSAGGSCIIVSYGLMGIVLSTYR"
BASE COUNT         1170 a          648 c          943 g         1086 t
ORIGIN      
        1 ttgcggatgt ttcagaacgc attcagtacc tggtaactca ggtggaagaa attactaaaa
       61 tagataattt tggagcgtat ctgactgcaa tgctggaaat agatgcgttt ttcatgaatg
      121 aggacagaca taccaacaat attgcggtca tttataatga aaaaacacag aaatatgaac
      181 tgagtccttt ttttgatcag ggactttgta tgtttgccga cataaatcag gattatccta
      241 ttgatcagtc attggaatat tgtctggaaa aaattgaggc gaagccattt agctctgatt
      301 ttgatataca gctggatgca gcagaagaac tgtatggcat tcagattggt ttccaattta
      361 cctttaaaga tattcagata tacttacaga agaattcgga aaattatccc agggaagtaa
      421 taagacgcgt ggagcagctg ttaagagggc agatgcgtaa atatgggtat ctgatgaaaa
      481 aatgatatta cagagagtgt atgaagttac aggcagataa aggataaaaa agtcacttat
      541 ctgccttttc tggtaaactt tttgtaacgg ctgtgaggcg gagactgtca aatcagtcaa
      601 tgatactata ctgcatcatt tcataatgta atctgcatcc ttctgtaatc tcaaagggca
      661 gaagtgcccg tgctacgagt tttaactctt cttcaggcag atctgttctg cgaagatgtg
      721 catagtcacc gtcaatagat tctacgatat aatcacaaac taacataaaa atcctccact
      781 ttatattttg cgtaacgcaa tgatcatatc tatagaatac cacagaggaa aagggtatca
      841 caagtgcctt cagattatat agacataaaa agagggcagt gttataattt ttcctaaaga
      901 aaatgcatat gggaggtatc gcaatgtcta aagttgtaaa gctttcatcc gtaatggaaa
      961 gggaagaaaa attaaaggaa atctatgaag gtctggaaga gataaaagat cagctgacag
     1021 cactgattga tgagtatgaa gaagaggaga cacatacagg aaaagcagat gaactgatcg
     1081 aagccctgga tgctcttgag gatgcttcgg aaaccataga ggatgtgctg gaaaattaat
     1141 gaaggagaaa gcatattttc acctgcccgg gctgtttgag ttttatgagc tgtaccggat
     1201 gttcctgcct ttattccggg aacacaggga atatttttac gactggtgtg aaattggctc
     1261 catatatggt gctccggcag actgtatctg gggtggaggc agggctggct tcggagagaa
     1321 tgacccggag gaggttcttg aactgatgca ggaatatggg atttctgccc gactgacttt
     1381 cagcaattcc ctgcttcgcc aggagcacct ttcagacaga aaatgcaatg cactgtgccg
     1441 gttgtttgag agaaacagag aaccgcaaaa cggcgtgatc atttattcag aattgctctt
     1501 ggaatatatt aaggagcagt atccgggact ctattttgtt tcctccacta ccaaggttct
     1561 gacagatttt acacagcttg aggaagaaat caggagaaaa gatttccatt atgttgtgcc
     1621 ggatttccgg ctaaacaagg cttttgataa gttaaataca ttgtctcagg ctgagaagga
     1681 caaagtagaa tttctctgca atgaatgctg ctggttcggc tgcagggaca gaaaagcctg
     1741 ctatgaaacg gtcagccgca agaatctggg tgaaaactgc ctggagcatt actgcaaggc
     1801 accggagaga gagaggggct atcttttttc aaaagctata gagaatccag ggtttattgg
     1861 aatcaatgat atccgggata tctatctgcc aatgggattt tcaaatttta aaatagaagg
     1921 aagaggactc ggcagtgccc tgatactgga atttctgctc tattacatga ccagaccgga
     1981 ataccagata catgtcagag aaaaaatata tctggacagt atgctggatc tgttttgaga
     2041 gatttatacc accgcattgt gttactatat gacaatgcgg tggtttttct gtccgaaaaa
     2101 gtatgtagat acctgaactc ggttgccata gcagtgaagt tttcaaaatt attccggaaa
     2161 cagttgacat agcatatagg ttcttatatt atatagataa ccgatatata gacaatctat
     2221 acaatgggag gtgaatacta tggctattga aaaatcactg gtttccggga gtatgacaat
     2281 gcttatactg aaattgctgt cagaaaagga tatgtatgga tatgagatga tcgataccct
     2341 gcgacagaaa tctcagaacg tgtttgaact gaaagcagga actctgtatc cgttactgca
     2401 cagtctggag gataagggat ttctgacagt atacgaacag gaggcagccg gaaagaccag
     2461 aaagtattac agtctgactg gacagggaag gggatttctg gaaaaaaaag tagaagaatg
     2521 gaaagagtat tctgcagcag tgaccaacgt gcttgtaatg gaggtgtgat ctatggacga
     2581 atatctgaaa acattactgg aacagattcg ctgtaagaaa gcgcggcctt atgtaaaaca
     2641 ggaatttcag gatcatatag aagatcagat cgaggcaaat atgcaggcag gtatggatcg
     2701 tgaacaggca gagagggaag ctgtccggga tatgggagat cctgtggaaa caggaatctc
     2761 tcttgacagt gtccacaggc ctcaggttgc atggaaactt ctggggataa tcgttcttat
     2821 cagcattgca ggcgttttga tacatgcagg gattgccgga aaaatcagtg agaacgctgc
     2881 ggccggttcg gacagatatg tgttccatgt tgtgatcgga cttgcagtta tgatgatttt
     2941 atatcttctg gattacactg tactggcaag attttcgaaa ataattgccg tcattttact
     3001 gtttgcctgt ctggttacat tgttgggtgg ctatcagtta aatggggcaa gatacttcat
     3061 tgtactgccg ggaggaagag gaatatccat gcagactctg atgctgtttt atgttcccat
     3121 atatggagca attttgtata aatatcatgg atggggatat aagggcttga tgagagcaat
     3181 catatggatg attgcaccgg ttatattggt ctatgccatg ccggcgctta tgccggcctg
     3241 catgatgctg gtatctatgc tggttatgct gacaatcgca attcaaaaga actggttcac
     3301 tgtgagaaaa aagaaagcca tctgcggaat ctgggctggt tttctggcta tgcctgtggc
     3361 agcttttctg atcaggtatc tgagcagcag tctgacagaa tatcagattg cacgtttaca
     3421 ggctattttt tcagatggtg gagaggaaga ttatttcacc ggaatgctac attcattctg
     3481 gcagcagaat aaatggatag ggaaaagcga atcagatgta atgggaaatc tgccggcttt
     3541 taatgcggat tatattttga cctatctgtc atctgtatat ggaacaattg cagccgtgct
     3601 tttatgctgt gttctggcag ttttgatttt cgcagttttt aacacttcca tgaggcagaa
     3661 gaatcagctt ggaatgatga tgggatgtgg atgcggaatt gtattccttg taaacttttc
     3721 cataaatatc ctggaaaatc tgggtgtatt tccacaatca gtaacatttc ttcccttttt
     3781 atcagcagga ggaagctgca ttatagtttc ctatggactg atggggattg tactgagtac
     3841 atacaga
//
LOCUS       DBMDXG010000188         3844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5517_length_3844_cov_1.874967, whole genome shotgun sequence.
ACCESSION   DBMDXG010000188 DBMDXG010000000
VERSION     DBMDXG010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5517_length_3844_cov_1.874967"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="ACZPD3_06145"
     CDS             complement(<1..481)
                     /locus_tag="ACZPD3_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1415612.1"
                     /translation="MSERKYPVITISREFGAGGHSIARVVSERLGIPYYDRDFAKQAA
                     ARSGYSQEDIDREGENLSRSARLLDNILNNSAGYFSSHDAIYQAQKELILQFAAEQDC
                     IIIGRCSNLILRNAGIPSLDIFLHADVNLRTEHIQKLGLNGKEDPRKYLTKMDNLRET
                     "
     gene            633..1634
                     /locus_tag="ACZPD3_06150"
     CDS             633..1634
                     /locus_tag="ACZPD3_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR1415613.1"
                     /translation="MRNKELFRGRRKAGRIVGLVLTLALAVSLLSGCGQSKERLTFGT
                     GGTAGTYYSYGGVLAQYITNNTNVKVTAVSTGGSKANLQSVQDGDFQLGFVQSDVMAY
                     AWDGVRAFEQDGATHDFRTLGGLYAETVQLFTMDPDIKTVEDLKGKSVSIGAANSGVY
                     FNALDVLNAGGITLDDIHPQYLSFEDSKESLKDGKIDAAFIVAGAPTTAITELATTNG
                     VYMINIDGAIRDSILSDCPYYTSYQIPAGTYPGQDEPVETITVKATIVVSESLDEDTV
                     YDMTAAIFDHADEISAENAKGAELSLENATSGMTIPFHKGAARYYAEHGITVDTKGE"
     gene            1640..3607
                     /locus_tag="ACZPD3_06155"
     CDS             1640..3607
                     /locus_tag="ACZPD3_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR1415614.1"
                     /translation="MKENKIKKANTSPEGQAGSENMSTADVDAVMKKYDRESNIRVWT
                     GKYKLAVRGLLVAFSLWCIYVTLFATFLEEIRLTSFLGLIILIGFLTYPAKKGDIREN
                     YMPIGDIIFMIAGTGAFLYFTFSANQIINQGTRFAPYQIVIGIIGIAALIELTRRCVG
                     LPILFVAGFFLIYALAVGLTNPAFLGRVKFLVRNLFYTKEGIFSRPVNVCSKYIVVFI
                     IFGAFLERTGISNFFIQLANCIAGKYAGGPAKVAVISSALCGMVSGSSVGNTVTTGSV
                     TIPMMKDTGYKPEFAGAVEAASSTGGQIMPPIMGAAAFLMADFVGVPYSNIIVRAILP
                     ALLYFTGIFISVHLEAKKLGLSGIPKEKLPVFKLLIRKIYLLLPLVMLVIWVSGNYMT
                     MQKAASYAIVLSVIVSLFDKENRISVTKCIDALEAGGRGVISVAVACGVAGIISGSIT
                     MTGLANDLINGIISVANGKLIIALLLTMLCCIVLGMGVPTTANYCIMAATCAPILVRM
                     GVPTLAAHFFVFYFGIVADITPPVALAAYAGSAIAKANPMKTAFTASKLAIAVFIVPY
                     VFCFNPAMLLIDTTPLKVVQIFITSLIGVFGLSSSLEGFLSVKMSVPVRVLMAAGGLM
                     LIDPSLMTDVVGILLIVGCCVWQTAQKKKTA"
     gene            complement(3668..>3844)
                     /locus_tag="ACZPD3_06160"
     CDS             complement(3668..>3844)
                     /locus_tag="ACZPD3_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HIR1415615.1"
                     /translation="YLPENMTVNSVTTAEYGASKAARPFNSRLDKSKLTANGFTPLPT
                     WQDALGRYLKELDF"
BASE COUNT          984 a          800 c          937 g         1123 t
ORIGIN      
        1 aggtttcacg caggttgtcc attttggtga gatatttcct gggatcttcc tttccgttta
       61 agcctaattt ctgaatatgt tctgtgcgga gattcacatc tgcatgaagg aaaatatcca
      121 gactgggaat accggcattt cgcaggataa gattggaaca gcggccgatg ataatacagt
      181 cctgttctgc tgcgaactga aggatcagct ctttctgagc ctggtaaata gcatcatggg
      241 aactgaaata gcctgcagag ttattcagaa tgttgtccag aagtctggcg ctgcggctca
      301 gattttcacc ctcacgatca atatcctcct gggaatatcc gcttctggca gcagcctgtt
      361 ttgcaaaatc ccgatcgtaa tatgggatac caagcctttc tgaaactact cttgcaatac
      421 tgtgtccccc ggcaccaaac tcccggctga ttgtgatgac agggtatttt ctttcggaca
      481 taatagatac ctccattggg ttgttattta ttttgttatc catgtttttc ctttattgta
      541 acgttttttg taagattatt caataaagat tatcattttt tgaaaaaata tgttataatt
      601 caaaatatta tgtacctgaa ggagatggat ttatgagaaa caaagagctt ttcagaggca
      661 ggagaaaagc tggcaggatt gttggccttg ttttgactct tgcacttgca gtcagccttc
      721 tgagcggctg cggacaaagt aaagaacgtc tgacgttcgg taccggcggt acggcgggaa
      781 cgtattattc ctacggtggt gtactggccc agtatattac caataataca aatgtaaagg
      841 ttacagcagt ttctacaggt ggttccaaag cgaacctgca gtcagtacag gacggagatt
      901 tccagcttgg atttgtccag tcagatgtaa tggcctatgc atgggacggc gtaagagcat
      961 ttgaacagga cggtgcaacc catgatttca ggactctggg cggcctgtat gcagagactg
     1021 tccagctgtt taccatggat cctgacatta agacagtgga agatctgaag ggcaagagcg
     1081 tatctatcgg tgcggcaaac tccggtgtat atttcaatgc tctggatgtt ttaaatgcag
     1141 gcggcattac cctggatgat atccatcccc agtatctgtc ttttgaggac agcaaggaat
     1201 ctttgaagga tgggaaaatt gatgctgcgt ttattgttgc aggtgcacct acaacagcta
     1261 tcacagagct tgcaactacc aatggtgtct atatgatcaa tattgatggc gcgatcaggg
     1321 attcaatttt gagtgactgt ccatattata catcctacca gattcctgca ggaacctatc
     1381 cgggacagga tgagcctgta gaaaccatca cagtcaaggc gacgatcgta gtgtccgaat
     1441 ctctggatga ggatacagtt tatgatatga ctgcggcaat ttttgaccat gcagacgaga
     1501 tttctgctga gaatgcaaaa ggagcggagc tttctctgga aaacgctact tccggtatga
     1561 ccattccttt ccataagggt gcggcgcgtt attatgcaga gcatggaatt acagtggata
     1621 cgaaagggga atagtcagga tgaaagaaaa taagataaaa aaagcaaaca catccccgga
     1681 agggcaggct ggttctgaga acatgagcac agcagatgta gatgcagtaa tgaagaaata
     1741 cgaccgcgaa tcaaatattc gtgtgtggac cggcaaatat aagctggctg tacgcggcct
     1801 gctggttgcc ttttctctgt ggtgtatcta tgttacctta tttgcaacct tcctggagga
     1861 aatccgactg acttcttttc tggggctgat cattcttatc ggatttctta cctatccggc
     1921 gaaaaaagga gatatcagag aaaactacat gccgattggc gatattattt ttatgatcgc
     1981 aggcacaggt gcttttctgt attttacttt ttctgcaaat cagatcatca atcagggtac
     2041 cagatttgcc ccttatcaga ttgtgatcgg tattatcggt attgctgcac tgatcgagct
     2101 taccagacga tgcgtgggcc taccgatcct gtttgtggca ggttttttcc tgatctatgc
     2161 actggctgta gggcttacca atccggcatt tttgggaaga gtgaaattcc tggtgcggaa
     2221 tcttttctac acaaaagagg gtattttctc cagacctgta aatgtatgct ctaaatatat
     2281 tgtggtattt attattttcg gtgcgtttct ggagagaaca ggaatctcca acttctttat
     2341 tcagctggct aactgcattg caggaaaata tgcaggtgga ccggcaaagg tagcggttat
     2401 cagttctgca ctctgtggaa tggtatccgg ttcctctgta ggtaacacag taacaaccgg
     2461 ttctgttaca atcccgatga tgaaggacac aggctacaaa ccggaatttg ccggagcagt
     2521 agaagctgct tcttccacag gtggacagat catgcctccg atcatgggag ctgccgcatt
     2581 ccttatggca gattttgtgg gtgtaccgta ctccaacatt attgtcaggg cgattctgcc
     2641 ggcattgttg tactttacag gaatctttat ttctgtacat ctggaagcaa agaaattagg
     2701 actttccgga attccgaagg aaaaacttcc ggtatttaag cttctgatcc gtaaaatcta
     2761 tctgttatta cctctggtca tgctggtaat ctgggtgtcc ggaaactata tgactatgca
     2821 gaaggctgcc agttatgcaa tcgttctttc tgttatcgta agtctttttg acaaagaaaa
     2881 cagaatcagt gttaccaaat gtattgatgc gctggaagca ggaggaagag gtgttatctc
     2941 cgtagcagta gcctgcggtg ttgcaggaat catttccggt tccatcacca tgacaggact
     3001 tgccaatgac ctgatcaacg gaatcatcag tgttgcaaac ggaaaactga tcattgcact
     3061 gcttttgact atgctgtgct gtatcgtact tggtatgggt gtacctacca ctgccaatta
     3121 ctgtatcatg gcagcaacct gtgctccgat cctggtacgt atgggagtac ctacactggc
     3181 agctcatttc tttgtatttt actttggtat tgttgcagat atcacaccgc cggtagcgct
     3241 ggctgcctac gcaggttcag ccatagcgaa ggccaatccg atgaagactg catttacagc
     3301 ctccaagctg gctatcgcag tatttatcgt accttatgta ttctgcttta atccggcaat
     3361 gctgctgatc gataccacac cgcttaaggt tgttcagatc tttatcacct cactgattgg
     3421 tgtgtttgga ttgtcctcct cactggaagg cttcctgtca gtgaaaatgt ctgtaccggt
     3481 tcgtgtctta atggcagcag gcggactgat gctgatcgat ccgtcactga tgactgatgt
     3541 tgtgggaata cttctgatcg tgggatgctg cgtatggcag accgcgcaga agaagaaaac
     3601 tgcatagaga agaataaaaa aaccggcaaa acacctgtca tttgggtgtc ctgccggatt
     3661 tttttattta gaaatccagt tctttcagat accgtccaag tgcatcctgc caggtgggga
     3721 gtggtgtgaa accattggct gtcagtttgc tcttatccag gcggctgtta aacggtctgg
     3781 ctgctttgga agcgccatac tctgcagttg tgacggagtt gactgtcatg ttttccggga
     3841 gata
//
LOCUS       DBMDXG010000189         3840 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5524_length_3840_cov_1.371222, whole genome shotgun sequence.
ACCESSION   DBMDXG010000189 DBMDXG010000000
VERSION     DBMDXG010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5524_length_3840_cov_1.371222"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..519
                     /locus_tag="ACZPD3_06165"
     CDS             55..519
                     /locus_tag="ACZPD3_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1415616.1"
                     /translation="MSDNNLSQKKQKQYEVYVKAVTPVHSLPLNMCKAFFTGGVICVV
                     GQVILNYADTLGLDKDTAGSWCSLILILFSVILTGCNLYSKIGRFGGAGSLVPITGFA
                     NSVASSAIEYKAEGQVFGIGCKIFTIAGPVILYGILSSWILGVIYYLFTMIP"
     gene            589..1671
                     /locus_tag="ACZPD3_06170"
     CDS             589..1671
                     /locus_tag="ACZPD3_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR1415617.1"
                     /translation="MNRQMTCGTSSISFQNPVFVQSCASVVSRKEGEGPLGAYFDTIC
                     EDPMFGTDTWEAAESTLQKQAALLAIKKNGLTCSDIQLLFAGDLLAQTSASSFGTADL
                     KIPFYGLFGACSTMGESLSLGSMCIDGGYGKYILCATSSHFASAEKEFRFPLGYGNQR
                     PLSATWTVTGAGACILGYEPPPRPDNKTLNTLRNRNICAVITGITTGRLIDFGFRDSL
                     NMGACMAPAACDTIARNLQDFHRKPSDYDAIFTGDLGMVGRTILFDLLTEKGFDISSV
                     HQDCGMLIYDPQTQDTHSGGSGCGCAASVLAGYILPGIIQKKWKRILFVPTGALLSKV
                     SFNEGDPIPGIAHAVVIEQYRVPDMM"
     gene            1702..2058
                     /locus_tag="ACZPD3_06175"
     CDS             1702..2058
                     /locus_tag="ACZPD3_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR1415618.1"
                     /translation="MDYFNAFWTGGLICALVQILLDRTKLMPGRIMVLLVCTGSVLSA
                     VGIYQPFAEFAGAGASVPLLGFGNILWKGMKESIDKNGLIGLFMGGFTACAVGVSAAL
                     IFSYLASLIFKPKMKG"
     gene            2065..2679
                     /locus_tag="ACZPD3_06180"
     CDS             2065..2679
                     /locus_tag="ACZPD3_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HIR1415619.1"
                     /translation="MSDTLYLQLDQNIQINHPHIYLQDIAKLSCSNSKILNRLRVMPV
                     INLDPNKPGRYVMSVMDLISEIKKKEPDLEINNIGEADFIITFKNKPGSGLVWQWCKI
                     IFVGLAAFFGAGFSIMTFNNDVDVGGLFSQIYTQVTGQTSGHFTVLEITYSIGIGLGV
                     LFFFNHFGHMKITDDPTPMQIQMRLYEENVNKTLIKDIDRTSEK"
     gene            2748..3245
                     /locus_tag="ACZPD3_06185"
     CDS             2748..3245
                     /locus_tag="ACZPD3_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR1415620.1"
                     /translation="MKNKGTQFLTEAAVIGAIYVVLTLLFAPLSYGEIQIRFSEALTI
                     LPFFTPAAIPGLFVGCIIANLFGGAIPVDIIFGSIATLIGAVFTYRLRSCNRFLAPIP
                     PIVSNAVIVPFVLHFGYGVNLPIPLMMLTVGIGEVVSCGVVGLILQTALLKYKNVIFR
                     SQRTV"
     gene            complement(3417..>3840)
                     /locus_tag="ACZPD3_06190"
     CDS             complement(3417..>3840)
                     /locus_tag="ACZPD3_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR1415621.1"
                     /translation="LKENCARIYSAREALEKELGREPILEEVAKATELSVDEVVMSME
                     SGAEVESLHKIIYQGDGNDISLMDRLQEKENGQDAALNRIFLDEILKKLDARERQLIG
                     MRYFKDMTQTEIAAEMGISQVQVSRMEKRILKELKKQV"
BASE COUNT         1016 a          908 c          789 g         1127 t
ORIGIN      
        1 tcagtaacct ggtgtgccaa aattccggat tttcaatcag aaaggtttta tattatgtca
       61 gataataatc tcagtcagaa aaaacagaaa caatatgagg tatacgtaaa agcagtcact
      121 cctgtccaca gcctgccact gaatatgtgc aaagcatttt tcacaggcgg agtcatctgt
      181 gttgttggac aggtcatctt aaattacgca gatacccttg ggctggacaa agataccgcc
      241 ggctcctggt gttcccttat cctgatcctt ttcagtgtga tcctcacagg atgtaatctt
      301 tattccaaaa tcggcagatt cggcggtgcc ggaagccttg tcccgatcac cggttttgcc
      361 aattccgtag cttcctctgc gatcgaatat aaagcagaag gacaggtatt cggcatcgga
      421 tgcaaaatat ttacgattgc aggacccgta attctttatg gaatcctgag ttcatggatt
      481 ctcggtgtga tttattatct ttttacaatg atcccgtaac tattcagcat ccatatcctg
      541 agaggtgttt ttgcataaga atcatacaat tatacaagga ggttttctat gaacagacaa
      601 atgacctgcg gtacatccag catttcattt cagaatcctg tctttgttca gagctgtgct
      661 tctgttgtca gccggaaaga aggggaaggg ccattgggcg catactttga taccatatgt
      721 gaagatccca tgtttggaac agatacctgg gaagctgcag aaagcactct gcaaaaacag
      781 gccgctctgc tggcaatcaa aaaaaacggc ctcacctgtt ctgatataca gcttcttttt
      841 gcaggcgacc tgctggctca gacatcagcc tcttctttcg gaaccgctga tctgaaaatt
      901 cctttttacg gtcttttcgg agcatgctcc actatgggag aatctctttc ccttggttca
      961 atgtgtatcg acggagggta cggaaaatat atcctctgtg caacttccag ccactttgcc
     1021 agcgcagaaa aagaatttcg ttttcccctg ggatatggaa atcaaaggcc cttatctgct
     1081 acctggacag tcaccggtgc cggagcctgt atacttggat atgaaccgcc accacgcccg
     1141 gacaacaaaa cactaaacac actccgtaac cgcaatatct gtgcagttat aacaggaatc
     1201 accaccggaa gactgattga ctttgggttc agggattccc tcaatatggg agcttgcatg
     1261 gctcctgctg cctgcgacac catagcccga aatcttcagg attttcaccg aaaaccatcc
     1321 gattatgatg ccatcttcac cggtgaccta ggcatggtag gacggacaat cctgttcgac
     1381 cttctcacag aaaaaggatt tgatatctca tctgtccatc aggactgcgg tatgctgatc
     1441 tatgatcctc agactcagga tacacattcc ggcggcagtg gatgtggatg tgccgcctct
     1501 gttctggcag gctatattct tccgggtatt atacagaaaa aatggaaaag gattctcttt
     1561 gttcccacag gtgcccttct gtcaaaggta agcttcaacg agggggatcc aattcccggt
     1621 atcgcacacg ctgttgtaat agaacagtac agggttccgg atatgatgta gatacagaaa
     1681 ttttttcagg aggaaaatta catggactat tttaatgcat tctggacagg cggactgatc
     1741 tgtgccctgg tacagattct tctggacagg acaaagctta tgcccggacg gatcatggta
     1801 cttctggtat gtacaggctc tgttttaagc gctgtcggaa tctatcagcc atttgcagaa
     1861 tttgccggag caggcgcttc tgttcctctg cttggttttg gcaatatcct gtggaaagga
     1921 atgaaagaat ccattgacaa aaacggactg atcggactct ttatgggagg ctttaccgcc
     1981 tgcgcggtgg gagtctccgc agctttgatc ttctcttatc ttgcaagcct gatcttcaag
     2041 cccaaaatga aaggataaca taccatgagt gatactctct atttacagtt agatcagaat
     2101 atccagataa accatccaca catttatctt caggacattg caaaactttc ctgcagcaac
     2161 agtaaaatcc tgaatcggct ccgggtgatg ccggttataa atcttgaccc gaataaacca
     2221 ggacgttatg taatgtctgt catggatctg atctccgaaa tcaaaaaaaa agaaccggac
     2281 cttgaaatca ataatattgg agaagccgac tttatcatca cttttaaaaa caaacccggt
     2341 tccgggcttg tctggcagtg gtgcaagatt attttcgtcg gtcttgccgc ctttttcggt
     2401 gcaggtttct ctatcatgac atttaataac gatgtagatg tcggtggtct cttctcgcag
     2461 atctataccc aggtcacagg acagacttcc ggtcatttta ctgttcttga gatcacttat
     2521 tccataggaa tcggacttgg ggtacttttc ttcttcaatc acttcggtca tatgaaaata
     2581 acagatgatc caacacctat gcagatacag atgcgtctgt acgaggaaaa tgttaataaa
     2641 actcttatca aagacattga ccgtacttcc gaaaagtgat atacttcgat agccgagtgt
     2701 tcgaaacctt ccactcgtaa aacaaatact aaaggagata atcaaatatg aaaaacaaag
     2761 gtactcagtt tctgacagaa gcagcagtga tcggagccat ttatgtggtt ctgactcttt
     2821 tattcgcacc actcagctat ggcgaaattc agattcgctt ttcggaagca ctgaccatcc
     2881 tgccattctt tacaccggct gcgattccgg ggttatttgt gggatgtatc attgccaatc
     2941 tctttggcgg tgccattcct gtagacatta ttttcggaag catcgcaaca ctgatcggag
     3001 cagtatttac ttacagactc cgcagctgca accgttttct tgcgccgatc ccgccgattg
     3061 tttccaatgc agtcatcgtg ccattcgtac ttcacttcgg atacggagta aatcttccga
     3121 ttccgcttat gatgcttacc gtaggtatcg gagaagtagt ttcctgcggt gtagtaggac
     3181 tgattcttca gacagccctt ctgaaataca aaaatgtgat tttcagatca cagagaactg
     3241 tataacttac gatatataac aataaagata ttatagcaaa aagagtgtgc aatattatag
     3301 taatccgcac actctttttg ttactgttcg ctccgttcac agtaacctct tttttattta
     3361 cctcttcaga gaatactcta ccttctacaa gcggtgagtt aattactccc atctgcttat
     3421 acctgctttt taagttcctt cagaatccgc ttttccatcc tggacacttg gacctgggaa
     3481 attcccatct ctgccgcaat ctccgtctgc gtcatatcct taaaatatct cataccgatc
     3541 agctgacgtt ctcttgcatc cagttttttc aggatttcat ccagaaaaat cctgttcagt
     3601 gctgcatcct gtccattttc tttttcctgg agcctgtcca taagcgaaat atcatttcca
     3661 tctccctgat agataatttt gtgcaaagat tccacttcag ctcctgattc catcgacatc
     3721 accacttcat ctactgaaag ttctgtcgct ttcgcaactt cctccagtat cggttcacgt
     3781 cccagttctt tttccagtgc ttctctcgca gaataaattc tggcacagtt ctctttcagg
//
LOCUS       DBMDXG010000190         3832 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5536_length_3832_cov_1.880461, whole genome shotgun sequence.
ACCESSION   DBMDXG010000190 DBMDXG010000000
VERSION     DBMDXG010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5536_length_3832_cov_1.880461"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="ACZPD3_06195"
     CDS             <1..295
                     /locus_tag="ACZPD3_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1415622.1"
                     /translation="NHNLKEDLSVETLSARYYVSKYHLMRKFKQETGYTLHNYIVNKR
                     LLMARTLISNGMPVTKAAQESGFAEYSTFSRAYRKQFKTNPSEELPHYSNPLK"
     gene            complement(425..1891)
                     /locus_tag="ACZPD3_06200"
     CDS             complement(425..1891)
                     /locus_tag="ACZPD3_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR1415623.1"
                     /translation="MKRESFGSRLGFILVSAGCAIGIGNVWKFPYICGAYGGAAFILI
                     YLCFLVILGIPVLVAEFAVGRGSHTSVAKCFDKLAPAGSCWKPLKFIGIIGCYMLMMF
                     YTTVGGWMIYYCVKSLRGDFVGATPEAVTQSFSDMLGNAPTMMLWMVIICLIGFAVCF
                     MGLQNGIEKISKVMMSALLIIMVILAVHSVMMEGAGEGISFYLIPDFQKIKEAGVGNV
                     VFAALSQSFFTLSIGIGAMLIFGSYLDKSRSLTGEAVSITVLDTFVALTAGFIIIPAC
                     FSYGIQPDAGPSLIFITLPNIFAKMSGGALWGSLFFLFLTFAAVSTIIAVFENLIVFN
                     MELFGWNRKKSVVVCAVLVVILSIPCVLGFNVLSGFQPFGDGSNIMDLEDFIVSNNLL
                     PLGSLGYLLFCTRKNGWGWDNFIAEVNAGTGMKFPKCLKNYVAYGIPAIIIVIYLKGY
                     YDKFAGLGTAALAGWMIVALVLLGFVFYCATAKKKVKQ"
     gene            complement(2174..>3832)
                     /locus_tag="ACZPD3_06205"
     CDS             complement(2174..>3832)
                     /locus_tag="ACZPD3_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1415624.1"
                     /translation="QHNITLALRAHNLMHKDQDYVVKDDEILIVDEFTGRIMPGRRYS
                     DGLHQAIEAKEHVKIKRESKTLATITFQNFFNKYDKKGGMTGTAVTEEKEFRDIYAMD
                     VVEIPTNRPVIRVDHEDAVYMTKKEKFNAVVNAVVEAHAKQQPVLVGTITIETSELLS
                     RMLKRQGIKHNVLNAKFHELEAEIVSQAGQAGAVTIATNMAGRGTDIKLDDVARNAGG
                     LMIIGTERHESRRIDNQLRGRSGRQGDPGESRFYISLEDDLMRLFGSERLMKIFTSLG
                     VAENEQIEHKMLSNAIEKAQEKIEFNNFGIRKNLLDYDQVNNEQREIIYKERRQVLDG
                     ENMREAIYKMIQDTVDTYVDMCFSDDVDSEEWDLNEFNGVLTPIIPIRPLTAESVKGK
                     KRDEIRHELKEEAVKLYEEKEAEFPEPEQLRELERVVLLKCIDSKWMDHIDDMEILRQ
                     GIGLAAYGQRDPVVEYKMSAFDMFNEMITSIQEDTLRMLYHVHVEQKIEREQVAKVTG
                     TNKDDSAGPKKPVQRKEIKVYPNDPCPCGSGKKYKQCCGRKNKA"
BASE COUNT         1096 a          975 c          686 g         1075 t
ORIGIN      
        1 caaccacaat ctgaaagaag atctatctgt agaaacgctt tctgccagat attatgtaag
       61 caagtaccat ctgatgcgga agttcaaaca agaaaccggc tatacccttc ataattatat
      121 tgtaaacaag cggcttctta tggcacgtac tctgatctca aatggaatgc ctgtgacaaa
      181 agcagcacag gaaagcgggt tcgcagaata cagcactttt tcccgggcat accgtaaaca
      241 gttcaaaacc aaccccagcg aagaactgcc tcattatagc aatcctctaa aataatgcat
      301 ccactacctt tacacaacaa ttccataaca caaaaaagat gtaaccatgt ctgaaagaat
      361 ctttcttccc agacataatt acatctttta ttgttctcag gtcgagcaga tattcgcgct
      421 ccgcttactg tttcactttc ttcttcgctg tcgcacagta gaatacgaat cccagcagta
      481 ccagtgccac gatcatccat ccggccagtg ctgctgttcc cagacctgca aatttatcat
      541 agtaaccttt cagatagatc acaatgataa tcgccggaat tccataggca acataatttt
      601 tcagacattt cggaaatttc atgcctgtac cggcattcac ctctgcaata aaattgtccc
      661 atccccatcc gttctttctg gtacaaaaca gaaggtaacc aagacttcca agaggaagaa
      721 ggttattaga aacaataaag tcttccagat ccatgatgtt gcttccatct ccgaatggct
      781 ggaatccgga aagtacattg aatccaagta cacatggaat gcttaagatc acaacaagaa
      841 ctgcgcagac aacaacactc tttttacggt tccagccaaa cagttccata ttaaacacga
      901 tcaggttctc aaatacagca atgatcgttg aaaccgcagc aaatgtcaga aacaggaaga
      961 acagacttcc ccacaaagcc cctccgctca tctttgcaaa aatattgggc agagtgataa
     1021 agataaggct tggacctgca tccggctgga ttccatagga gaagcatgcc ggaataatga
     1081 taaatcctgc tgtcagcgca acaaaagtat cgagtacggt gatgcttacc gcctctccgg
     1141 taaggcttct ggatttgtcc agataacttc cgaagatcag catggcacca ataccaatgg
     1201 ataatgtaaa gaatgactga ctcaatgccg caaatactac attaccaact cctgcttcct
     1261 taatcttctg aaaatcagga atcagataga agctgattcc ttctcccgcg ccttccatca
     1321 ttactgaatg aaccgcaagg atcaccataa tgataagcag tgcactcatc attactttgg
     1381 agattttttc aataccattc tgcaatccca tgaaacagac tgcaaatccg atcagacaga
     1441 tgatcaccat ccataacatc atcgtcggtg catttcccag catatcggaa aaagactgtg
     1501 ttactgcttc cggagtcgct ccaacgaaat ctccccgcag actcttcaca caatagtaga
     1561 tcatccatcc tcctactgtt gtgtaaaaca tcatcagcat ataacatcca ataataccaa
     1621 taaatttcaa tggcttccag cagcttcctg caggtgccag tttgtcaaaa catttcgcaa
     1681 cacttgtgtg gcttccccgt cctaccgcaa attctgctac cagaaccgga ataccgagaa
     1741 taaccagaaa acataagtag atcaggataa aggccgcgcc tccatatgcc ccgcagatat
     1801 acggaaactt ccacacgttt ccaataccga tggcacatcc tgctgacacc aggataaaac
     1861 caaggcgtga gccgaaagac tctcttttca tactgttttt ccccctttat ggaattgtta
     1921 tgtgactgtt catcaatcat aatcacatag ctgtggatgt actgaacagt tcagacagat
     1981 attcacaatc tgccaattca acgcgctaac gtgtttttgc gcacgataaa agggcctcat
     2041 gattcagccc ttttattttg cagcagacat gatctgctca caatgttctg tttacaaaat
     2101 cattactgcc cgctctgtag taatgaactc accacttaca gaaatgggaa tcttctcaac
     2161 tgagataaac gagtcaggcc ttatttttac gtccacagca ctgtttgtat ttcttaccgc
     2221 ttccacatgg gcagggatcg ttcggatata ctttgatttc ttttctctga accggtttct
     2281 taggaccggc actgtcatcc ttgtttgttc cggtaacttt agctacctgt tcacgctcga
     2341 tcttctgctc tacatgtaca tggtacagca tacgaagagt atcttcctga atggatgtga
     2401 tcatctcgtt gaacatatca aatgcactca ttttatattc aacaaccgga tctctctgtc
     2461 cataagcagc aagtccgata ccctgacgaa ggatttccat atcatcaatg tgatccatcc
     2521 acttgctgtc aatgcatttc agaagtacaa ctcgctctaa ttcacgaagc tgctccggtt
     2581 ccgggaactc tgcttctttt tcttcataga gcttcactgc ctcttctttc agttcatgtc
     2641 tgatctcatc gcgtttcttg ccttttacac tctcagctgt aagcggtctg atcggaatga
     2701 tcggagtaag aacaccgtta aattcattca gatcccactc ttcagaatct acatcatcag
     2761 agaaacacat atcaacataa gtatccactg tatcctggat catcttatag attgcttcac
     2821 gcatattctc accgtcgaga acctgacgac gttctttgta gatgatctca cgctgttcat
     2881 tgtttacctg gtcataatca agaagattct tacggatacc aaagttgtta aactcaatct
     2941 tctcctgagc tttctcaatt gcattggaaa gcatcttgtg ctcaatctgt tcattctctg
     3001 caacaccaag agatgtaaag atcttcatca gtctctcaga accgaacaga cgcatcaggt
     3061 catcttccag ggaaatatag aaacgggatt cacccggatc tccctgacgt ccggaacgtc
     3121 cgcgaagctg attatcaata cgtctggact catgacgttc tgtaccgatg atcatcagac
     3181 cacctgcatt tcttgcgaca tcatccagct tgatatctgt accacgacca gccatgttag
     3241 tggcgatcgt aactgctcct gcctgacctg cctgtgagac aatctctgcc tcaagttcat
     3301 ggaacttggc attcaataca ttatgtttga ttccctgtct cttcagcata cggctgagaa
     3361 gctcggaagt ttcaatcgta atagtaccga ccagtacagg ctgctgtttc gcatgagctt
     3421 ccactacagc attgacaact gcgttgaatt tctctttctt tgtcatataa acagcatctt
     3481 catggtcaac tcggataacc ggtctgttag tcgggatctc tacaacgtcc attgcataga
     3541 tatcacggaa ttctttctcc tctgtgacag ctgtaccggt cataccgccc ttcttatcat
     3601 atttattaaa gaagttctgg aaagtgatgg ttgccagagt cttgctctca cgtttgatct
     3661 taacatgctc tttcgcttcg attgcctgat gaagaccatc tgagtaacgg cgtcccggca
     3721 tgatacgtcc ggtgaattca tcaacaataa ggatttcatc atctttaact acataatcct
     3781 gatccttatg catcaggtta tgggcacgaa gtgcaagtgt aatattatgc tg
//
LOCUS       DBMDXG010000191         3810 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5568_length_3810_cov_1.812079, whole genome shotgun sequence.
ACCESSION   DBMDXG010000191 DBMDXG010000000
VERSION     DBMDXG010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5568_length_3810_cov_1.812079"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="ACZPD3_06210"
     CDS             <1..266
                     /locus_tag="ACZPD3_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HIR1415625.1"
                     /translation="LAKDGTVIKYVAEKGCQAAYQYVEPGRGCEQHLHLKCVKCGKII
                     HLECHFMEEISHHIEESHGFTLQCKNSILYGVCKECKGSGEDC"
     gene            519..1418
                     /locus_tag="ACZPD3_06215"
     CDS             519..1418
                     /locus_tag="ACZPD3_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012863.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1415626.1"
                     /translation="MQTAKNSTVQKKKTSTKTVTIETSGGITKKKVKSGGSVILPVEV
                     NKRGYTFLGWSTVPGQTCNPMYQAYQKIQVTKNIHLYPVKYKWNQEPDIYAGGLADSV
                     EKYDKIIFVGDSRTAMLRSTLKQQCSSDSLKKVGFVCKTGEGLDWMKKYGEKELLNEI
                     SGMDDNAKPVAVIFNLGVNDLIHKNRESISYDSVASDYASYMNGLSRKLTARNCELFY
                     MSVNPCNTAMKSTRKESEIRGFNNRLRQRLNGNFTWINSYSYLMRCGYTTRCEFRGYT
                     DDGVHYSMRTYKRIYAYAIKQIR"
     gene            1551..2276
                     /locus_tag="ACZPD3_06220"
     CDS             1551..2276
                     /locus_tag="ACZPD3_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR1415627.1"
                     /translation="MRSESFRKGVKDGIPIGLGYFAVSFTFGMMAVADGLSIWQAVLI
                     SLTNVTSAGQFAGLEIMVMSGSYWEMALTQLIINLRYCLMSFSLAQKFRRDESLVHRY
                     IAAFGVTDEIFGISASQPGKVSAFYNYGAMCVAIPGWVLGTLAGAISGNLLPDFMMSA
                     LSVAIYGMFLAIIIPPAKQNKAVLAVVVAAMLISTLFKVIPFLSEVSSGFVIIITTLI
                     VAGAAAYFCPIEDEKEEEGVHES"
     gene            2266..2583
                     /locus_tag="ACZPD3_06225"
     CDS             2266..2583
                     /locus_tag="ACZPD3_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR1415628.1"
                     /translation="MSHNIYIYILVMAAVTYLIRMLPLALSRKEITSPFIRSFLYYVP
                     YACLAAMTFPAILFATDSVISAAVGFIVALIAAYKEKSLLTVALFACAAVFIVERILE
                     FAV"
     gene            complement(2711..3562)
                     /gene="dapF"
                     /locus_tag="ACZPD3_06230"
     CDS             complement(2711..3562)
                     /gene="dapF"
                     /locus_tag="ACZPD3_06230"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR1415629.1"
                     /translation="MKFTKMHGIGNDYVYVNCFEETVENPSAVARYVSDRHFGIGSDG
                     LILIKPSKIADCEMDMYNLDGSQGAMCGNGIRCVAKYAYDYGIVDKEHISVATKSGIK
                     YLDLTVENGKVSQVKVNMGSPILTARQIPVVSEKEEVINEPLDVNGKIYYITAVSMGN
                     PHAIVYMDDIDHLDIEKIGPAFENHVAFPDRVNTEFVEVIDEHTVKMRVWERGSGETL
                     ACGTGACAVAVASVLNGHVDGDSPVTVKLLGGDLQIFWNRQENLVYMTGPAATVFDGE
                     IDVSFLK"
BASE COUNT         1140 a          689 c          880 g         1101 t
ORIGIN      
        1 agcttgcaaa ggatggaact gtgatcaaat atgttgcaga gaaaggttgc caggctgctt
       61 atcagtatgt agaaccggga cggggatgtg aacagcatct acacctgaag tgtgtaaaat
      121 gtggaaagat cattcatctg gaatgtcatt ttatggaaga aatttctcat catattgaag
      181 aaagtcatgg atttaccctg cagtgtaaga attctatttt gtatggagtc tgtaaagaat
      241 gtaaaggcag tggcgaagac tgttgactgt ttctgtcaga tgatgtaaac tgaaatacaa
      301 ctgtttggtc aaattgactg cgaagcagta tggtgaagag aaaagacagc gaaattgctg
      361 taagtaccgg taaaaataac agataatata tgaaatcata aaagaaggca ataatgagaa
      421 aacagaagag acagatgtgt gtaagaagaa tgttattatt ttttctggcg tgtgtattaa
      481 tatgtgcagc acccccgtct gcagcacagg cggcaataat gcagactgct aagaattcaa
      541 cagtgcaaaa gaaaaaaaca tccacgaaaa cagttacaat agagacttca ggaggaatca
      601 caaaaaagaa agtgaaaagc ggtggttctg tgattcttcc tgtggaagtg aataagaggg
      661 gatatacatt tctgggatgg tcaacagttc ccggacagac ctgtaatcca atgtatcagg
      721 catatcagaa aattcaggtt acgaaaaata ttcatcttta tccggtgaag tataaatgga
      781 atcaggaacc ggatatttat gcaggcggtc ttgcggacag tgtggagaaa tatgataaga
      841 ttatttttgt gggagattcc cgcacagcaa tgcttcgcag tactctgaag cagcaatgca
      901 gttctgacag tttaaagaaa gttggatttg tatgcaagac aggagaaggg cttgactgga
      961 tgaaaaaata cggagaaaag gaactgctga atgaaataag cgggatggat gataatgcga
     1021 agccggtggc agttattttc aatctgggag taaatgacct gatccacaaa aacagggagt
     1081 ccatcagtta tgattctgtt gcgtcagact atgcttctta tatgaatgga ttatccagaa
     1141 agctgactgc caggaactgt gaattatttt atatgtctgt gaatccatgt aatacggcaa
     1201 tgaaatccac gcggaaggag tctgagatcc gcggatttaa taacagactc agacagagac
     1261 tgaacgggaa ttttacatgg atcaacagtt attcttatct gatgagatgt ggttatacga
     1321 ctcgctgtga atttcgcggt tatacagatg atggtgttca ttattctatg aggacttata
     1381 agagaattta tgcatatgca ataaaacaga tcagataatc ctgactgaaa cccggagaca
     1441 gaatactgtt ctccgggtta tctgtgtgta tcagtaaata cgaaagttgt agctgtgaaa
     1501 gagttaaaca gttatcaaag gatcacaaaa ggtaacagga ggagagaaac atgagaagtg
     1561 aaagcttcag aaaaggagtt aaggatggta ttcccatagg actgggatat tttgctgtat
     1621 cgtttacatt tggcatgatg gcagttgctg atggactgag tatctggcag gctgtgctga
     1681 tctcattgac aaacgtgact tcagcagggc agtttgcagg acttgagatc atggtaatga
     1741 gcggctctta ctgggagatg gcactgacac agctgatcat taatctgaga tattgtctga
     1801 tgtcattttc ccttgctcag aaattcagaa gagatgaatc tttggttcac cgctatattg
     1861 cagcatttgg tgtgacggat gagattttcg ggattagtgc cagtcagccc ggaaaggtaa
     1921 gcgcatttta taattatggt gccatgtgtg tggcaatccc gggatgggta cttggaaccc
     1981 tggcaggagc aatttccgga aatcttcttc cggattttat gatgagtgca ctcagcgtgg
     2041 ctatttacgg tatgttcctt gcaattatta ttcctccggc aaagcagaat aaggcagtac
     2101 tggcagttgt cgtggcagca atgctgatca gcactttgtt taaagtgatt ccgtttctgt
     2161 cggaggtttc gtcaggtttt gtgatcatta ttactacact gatcgtggca ggggctgccg
     2221 catacttctg tccgatagag gatgagaaag aggaggaggg tgttcatgag tcataatatt
     2281 tatatttaca ttctggtaat ggcagcagtt acatatctga tccgtatgct cccgcttgct
     2341 ctttccagaa aagagatcac aagtccgttt atcagatcat ttctgtacta tgttccctac
     2401 gcatgtctgg cagccatgac atttccggcg atcctttttg ccacagacag tgtgatctcg
     2461 gctgctgtgg gatttattgt ggcactgatc gcggcatata aggagaaaag tcttcttaca
     2521 gttgcgttat tcgcatgtgc tgcggtattt attgtggaaa gaatactgga atttgcagtg
     2581 taaaagttga taaaaaacgg atacataacc acagctgaat ggtgtgtatc cgttttttgt
     2641 tgcgtaatat tgtgaccggc agctgaagca gataagctgt ctgttatttg ctgcagtttc
     2701 agacagtttt ttatttcaga aagcttacat caatttcacc gtcaaatact gtggcagccg
     2761 ggcctgtcat gtaaacaaga ttctcctgtc tgttccagaa aatctgcagg tcaccgccga
     2821 gaagttttac agtgacagga ctgtcaccgt ctacatgtcc gttcagaaca gaagcgactg
     2881 ctaccgcaca tgcacctgtg ccgcaggcaa gagtttcgcc tgaaccgcgt tcccatacac
     2941 gcatttttac agtatgttca tcgatcactt ctacaaattc agtgtttaca cgatccggaa
     3001 atgcaacatg attctcgaaa gccgggccga tcttttcaat atccagatgg tcaatatcat
     3061 ccatataaac aatagcgtgg ggatttccca tggaaacagc ggttatgtaa tagatttttc
     3121 cgtttacatc cagaggttca ttgataactt cctccttctc tgacactacc ggaatctgtc
     3181 tggcggtcag gatcggagaa cccatattta ccttaacctg ggataccttt ccgttttcta
     3241 cagtaagatc cagatatttg atgccgcttt ttgtagcaac agaaatatgt tctttgtcta
     3301 cgatgccgta gtcataggca tatttggcaa cacagcggat accgtttccg cacatggcac
     3361 cctgggaacc gtcaagatta tacatatcca tctcgcagtc tgcgattttg gatggcttga
     3421 tcaggatcag tccgtcggaa ccgataccaa agtgacggtc actgacatat ctggctactg
     3481 cggaaggatt ttccacagtt tcctcaaaac agtttacata tacgtaatca tttccaatac
     3541 catgcatttt tgtaaatttc atcagaaaag cctccttatt tggttaaata tttatacacc
     3601 tcttgtatca tatagaaaaa atgttatgaa aaagtgaaaa ttacgaatct tttaccggga
     3661 aagtttttct gtgaccttca tgtaaaaaaa cattttgccc ttcccggaac agttatgtat
     3721 tatacattat atggaaaata tatctgttat tcaagtttta ccttgcattt tatagaaagt
     3781 atgttaaaat taaaaacaaa aatatacaac
//
LOCUS       DBMDXG010000192         3782 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5607_length_3782_cov_2.420538, whole genome shotgun sequence.
ACCESSION   DBMDXG010000192 DBMDXG010000000
VERSION     DBMDXG010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5607_length_3782_cov_2.420538"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="ACZPD3_06235"
     CDS             <1..837
                     /locus_tag="ACZPD3_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR1415630.1"
                     /translation="FVLSDADKTKRPSVEALLHNLFAYTYVLHVHPTLVNGLTCGKGA
                     KELSEQLLGKDVLWIDICKPGYTLARICYEKMNAYKEEYGKDVQVLLLQNHGIFVAAN
                     TVEEIGALFDGVISKLEKQVKRTADVSDAVTSEKEQAAEKLSRLLGHAVEVVPAAEAD
                     HFVKDKAAAAPLLKPFTPDHIVYCGPYPLFVEDIDQAKAALDAFMAENDKEPRLILVQ
                     GVGAFIMEDDKGKAAKAQLLVKDAIKLAVYAESFGGPLHMTDDITYFITHWEAEAYRS
                     KK"
     gene            complement(927..3584)
                     /gene="adhE"
                     /locus_tag="ACZPD3_06240"
                     /gene_synonym="adhC"
     CDS             complement(927..3584)
                     /gene="adhE"
                     /locus_tag="ACZPD3_06240"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HIR1415631.1"
                     /translation="MAKKENTIPTIIDTPEALTAKIAAMKEAQKIFATFTQEQVDKIF
                     KAAATAADKARIPLAKDAVEETGMGIVEDKVIKNHYAAEYVYNAYKNTRTCGVIEEDT
                     AYGIKKIAEPIGLIAAVIPTTNPTSTAIFKTLIALKTRNAIIISPHPRAKKCTIEAAK
                     VVLEAAVEAGAPEGIIGWIDVPSLDLTNQVMREADIILATGGPGMVKAAYSSGKPALG
                     VGPGNTPVIIDDSADIRLAVNSIIHSKTFDNGMICASEQSVTVLESIYKKVKEEFLYR
                     GCYFLKPDELEKVRKTILINGALNAKIVGQKAATIAEMAGITVSPETKILIGEVESVD
                     ISEEFAHEKLSPVLAMYKAKTFDEAIAKAEQLVADGGYGHTSALYIDTREKEKMEKHA
                     AAMKTCRILINTPSSQGGIGDLYNFKLVPSLTLGCGSWGGNSVSENVGVKHLINIKTV
                     AERRENMLWIRTPEKVYFKKGCLPVALDELGTVMHKKRCFIVTDSFLYKNGYTKKIED
                     KLDQMGIVHTCFYDVEPDPSLASARAGAAAMRMFEPDCIIAMGGGSAMDAGKIMWVLY
                     ENPDANFDDMAMDFMDIRKRIFTFPHMGKKAYFIAVPTSSGTGSEVTPFAIITDKTTG
                     IKWPLADYELMPDMAIVDTDNMMSAPKGLTCASGIDVMTHALEAYASMMASDYTDGLA
                     LNAIKLVFNYLPRAYKDGSDVEARQHMANASCMAGMAFANAFLGVNHSLAHKLGAFHH
                     LPHGIANALVLLHVLRYNAAEVPAKMGTFPQYQYPHTLARYAEIGRSVGLTGKNDSEV
                     FEKLLQKLQELMDTIEIKHTIKEYGVDEKYFLETLDEMTEQAFNDQCTGANPRYPLMS
                     ELKEIYLKAYYGEGNIPESGKAKEKKEEK"
BASE COUNT         1045 a          791 c          828 g         1118 t
ORIGIN      
        1 tttgttttgt cagatgctga caaaacaaaa cgtccaagtg tagaagcact gcttcataac
       61 ttatttgcct atacttatgt actgcatgtt catcctacac ttgtcaatgg actgacctgc
      121 ggaaaaggcg caaaggaact cagcgaacag ctccttggaa aagatgtact ctggatcgat
      181 atctgcaaac ccggatatac gcttgcacgt atctgctatg agaaaatgaa tgcttataaa
      241 gaagaatacg gaaaagatgt tcaggttctt ctgctccaga atcacggaat tttcgttgca
      301 gcaaatactg tagaggaaat tggagcatta tttgacggcg tgatcagtaa attagagaaa
      361 caggtaaaga gaactgctga tgtcagtgat gctgttacat ctgaaaaaga acaggcagca
      421 gagaagcttt cccggttact gggacatgca gtagaagttg tacctgcagc agaagctgat
      481 cattttgtaa aagataaagc agcggctgca ccgcttctga agccgtttac accggatcac
      541 attgtatatt gcggaccata tccgttattt gtagaggata ttgaccaggc aaaagctgcg
      601 ctggatgcat ttatggcaga gaatgacaaa gagccaagac tgatcctggt acagggagtt
      661 ggcgccttta tcatggaaga tgataaggga aaagctgcaa aagcacagct tcttgtaaaa
      721 gatgccatta agcttgcagt atatgcagaa agctttggtg gcccgcttca catgacagat
      781 gatattacat actttatcac gcattgggaa gcagaagcat atcgcagtaa gaaataatat
      841 aataagggct gtcgtttttt gacagccctt attatattgg tttaaacact atatttttaa
      901 aggaaaaggt ttattttcag caaatcttat ttctcttctt ttttctcttt ggctttcccg
      961 gattcaggaa tatttccctc accatagtaa gctttcagat agatttcctt cagctcagac
     1021 attaatggat atctcgggtt ggcaccggta cactggtcat taaatgcctg ttcggtcatt
     1081 tcgtcaagag tttcgaggaa gtacttctcg tctacgccgt attctttgat cgtatgtttg
     1141 atctcaatag tatccatcag ttcctgcagt ttctggagaa gcttctcaaa tacttcgctg
     1201 tcgttcttgc ctgtcagacc tacagaacgg ccaatctcag cgtaacgtgc aagtgtgtgc
     1261 ggatactgat actgtgggaa ggtacccatc ttagctggaa cttcggctgc attatagcgc
     1321 agaacatgaa gcagaaccag agcatttgca ataccatgtg gaaggtgatg gaaagctccc
     1381 agcttatgag caagagagtg gtttacgcca aggaaagcat ttgcgaaagc cataccagcc
     1441 atgcaggaag catttgccat atgctgtctt gcttctacat cagaaccatc tttgtatgca
     1501 cgtggcagat agttaaatac cagtttgatt gcatttaatg caagaccatc tgtataatca
     1561 gatgccatca tggaagcata ggcttcaagt gcatgagtca taacgtcgat accggaagca
     1621 caggtcagtc ccttaggtgc gctcatcata ttatctgtat caacaatagc catgtcaggc
     1681 attaattcat aatcagccag aggccatttg atacctgtag tcttatcagt aatgatagca
     1741 aacggcgtta cttcggaacc tgtaccggaa gatgttggaa cagctataaa gtaagctttc
     1801 ttacccatat gcgggaatgt gaagattctc ttacggatat ccataaagtc catagccata
     1861 tcatcaaagt tggcgtccgg attttcatag agaacccaca tgatcttgcc ggcatccatt
     1921 gcggaaccac cgcccattgc gataatgcag tcaggctcga acattctcat tgcagcagca
     1981 ccggctcttg cagaagcgag agatggatct ggctctacat cgtagaagca ggtatgtacg
     2041 attcccatct gatctaattt atcttcgatc tttttagtat atccattctt ataaaggaaa
     2101 gagtctgtta cgataaagca gcgtttcttg tgcataacag tgccaagctc atccagagca
     2161 accggcaggc agcctttttt gaaataaact ttttccggtg ttctaatcca gagcatattt
     2221 tctctccttt cagcaacggt cttgatgttg ataagatgtt tcacacctac gttctcagat
     2281 acggagttgc caccccagga accacagccc agagtcaggg acggaacaag tttgaagttg
     2341 taaaggtcgc cgatgccgcc ctgagaagat ggagtattga tgaggatacg gcaggtcttc
     2401 atagctgctg catgtttttc catcttttcc ttctctcttg tgtcaatgta gagagcggag
     2461 gtatgtccat aaccgccgtc agctacaagc tgttcagctt tggcaatcgc ttcgtcaaaa
     2521 gttttggcct tgtacatggc aagtacaggt gaaagcttct catgtgcgaa ttcttcgctg
     2581 atgtctacag attcaacttc gccgatcagg atctttgtct caggagaaac tgtaataccg
     2641 gccatctcag cgatagtggc agctttctga cctacgattt ttgcattcaa tgcaccatta
     2701 atgaggatag tttttcttac tttctctaat tcatccggtt tcaggaagta acatcctctg
     2761 taaaggaatt cttcttttac tttcttataa atgctctcaa gaacagttac agattgttcg
     2821 gaagcacaga tcataccatt gtcaaaggtc ttggaatgga tgatggagtt aactgccaga
     2881 cggatatctg cactgtcatc aatgataaca ggagtatttc cgggacctac acctaaagca
     2941 ggttttccgg aagaataggc agccttaacc attccgggac cacctgtggc aaggatgatg
     3001 tctgcttcac gcataacctg atttgtcaga tcaagacttg gaacatcgat ccatccgatg
     3061 attccctccg gtgcaccggc ttctacagct gcctccagaa caacctttgc tgcctcaatg
     3121 gtacatttct ttgcgcgggg atgcggactg atgatgattg cattacgtgt tttcagtgcg
     3181 atcagagttt tgaaaattgc agtagatgtt gggttggttg taggaataac tgcagcgatc
     3241 agaccgattg gctcagctat tttcttaata ccgtaagcag tatcttcttc aatcacaccg
     3301 caggttctgg tattcttata tgcgttgtat acatattcgg ctgcgtaatg gttcttgata
     3361 accttatctt ctacaatacc cattcctgtt tcttcaacag catctttagc aagcgggata
     3421 cgtgctttgt cagcagcagt agcggcagct ttaaaaatct tatctacctg ctcctgggta
     3481 aatgttgcga aaatcttctg tgcctctttc atggctgcaa tctttgcagt aagggcttca
     3541 ggggtgtcga tgatggttgg gatagtgttc tctttttttg ccatttggga atcctcctgt
     3601 ttgtgttttc gttaaaagta tttaccgtga cctgttatgt ttttattata caaataaact
     3661 gattattcgg tcaacagtaa cattgatatg agcataacga aatattatgg tgagcattgt
     3721 gcataatcac ataaataatg tgaataaaat atcaattgtc agaatatatg agaaaaattt
     3781 aa
//
LOCUS       DBMDXG010000193         3773 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5620_length_3773_cov_1.594227, whole genome shotgun sequence.
ACCESSION   DBMDXG010000193 DBMDXG010000000
VERSION     DBMDXG010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_3773_cov_1.594227"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..374
                     /locus_tag="ACZPD3_06245"
     CDS             3..374
                     /locus_tag="ACZPD3_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR1415632.1"
                     /translation="MTYKIQDLGGIGEVQIADEVVAIIAGLAATEVNGVASMAGNITN
                     ELVSKLGMKNLSRGVKVTVLEGVVTVDLNLNIEYGKNILETSKKVQEKVKSSIENMTG
                     LEVADVNIHIASVDMENEKGK"
     gene            439..861
                     /gene="nusB"
                     /locus_tag="ACZPD3_06250"
     CDS             439..861
                     /gene="nusB"
                     /locus_tag="ACZPD3_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR1415633.1"
                     /translation="MRRREQREHIFKLLFMTEFNSEEEMSEQLSLYFDTLEELSEKDQ
                     EYMSRKYRHVLEKLDEIDALLNETSNGWKTKRMSRVDLTALRLAVYELKYDKDVPTGV
                     AINEAVELAKRFGGETSGSFVNGILGKIANSESEEKSE"
     gene            858..2087
                     /gene="xseA"
                     /locus_tag="ACZPD3_06255"
     CDS             858..2087
                     /gene="xseA"
                     /locus_tag="ACZPD3_06255"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006864262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR1415634.1"
                     /translation="MKNTYSVGQVNRYIKNMFTQDYLLQKIYVKGEVSNCKYHTSGHI
                     YFSLKDETGTLNCVMFAGHRRGLAFAMKNGDKVIVGGSVDVYERDGRYQMYAKEITLE
                     GAGTLYERYLALKQELEDMGMFAQEYKQPIPRFIRRLGVVTAPTGAAVQDIRNISYRR
                     NPYLQIILYPALVQGAGAAESIVKGIQMLDKTDVDVIIVGRGGGSIEDLWAFNEEIVA
                     RAIFECSTPIISAVGHETDFTIADFVADLRAPTPSAAAELAVDDYRSVIEAVSIYRQR
                     LYRAMSGKTDLYRNRLEHFQTKFAYLSPENRLREQRQRLADLENAVQNGMNRKLQDER
                     HRLSVYLERFAGLSPLKKLNQGYSYVADKYKKTLTSVEQVQNGDTIYISVTDGTIEAD
                     VTGTVKEERIYGEERKL"
     gene            2065..2304
                     /gene="xseB"
                     /locus_tag="ACZPD3_06260"
     CDS             2065..2304
                     /gene="xseB"
                     /locus_tag="ACZPD3_06260"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR1415635.1"
                     /translation="MAKKENCEQLQEEKSLEEMFAELDLLAEKLENRDTSLEDSFILY
                     RQGMELLKLCSGKLDTVEKKMLQLNEDGTFSEFSR"
     gene            2288..3175
                     /locus_tag="ACZPD3_06265"
     CDS             2288..3175
                     /locus_tag="ACZPD3_06265"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR1415636.1"
                     /translation="MNFQDELAKRTEETEKVIRSFLPAEAGFAGTMAQAMNYSMLAGG
                     KRLRPMLIRETYRLFDGKEEVVKPFMAGMEMIHTHSLIHDDLPALDDDDYRRGRLTTH
                     KVYGEAMGVLSGVALLNYAYETMFQAFALAKEQDRVIHALRVVSQKTGIHGMLGGQSV
                     DVENDGKPLEKEMLDYIYRNKTSALIEASMMTGAILAGANEQEVSAVEKAAGNIGLAF
                     QIQDDILDVTSTAEELGKPVHSDEKNNKVTYVTLFGTEKAAEQVEELSEKAIDLLKSL
                     NKNNEFLYLLIEKLINRRK"
     gene            3189..>3773
                     /locus_tag="ACZPD3_06270"
     CDS             3189..>3773
                     /locus_tag="ACZPD3_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HIR1415637.1"
                     /translation="MILEKIKKPNDIHKIPLEDFEPLAAEIRDFLIRSVSQTGGHLAS
                     NLGVVELTLALHNVLDFPEDKLIWDVGHQAYTHKILTGRKDEFKNLRQEGGLSGFPKR
                     SESPCDAYDAGHSSNSISAGLGYVHARDILGQKHHVVSVIGDGALTGGMAYEALNNAA
                     ELKTNFIIIINDNNMSISRNVGGMSTYLSALRTAE"
BASE COUNT         1215 a          622 c          991 g          945 t
ORIGIN      
        1 gaataacata taaaatacag gatttaggag gcattggtga ggtccagatt gcagatgaag
       61 tggtcgctat cattgccgga cttgctgcta cggaagtaaa cggagttgct tccatggcag
      121 gcaatatcac aaacgaactg gtaagcaaac ttggaatgaa gaatctttcc agaggtgtta
      181 aggttactgt actcgaagga gttgtaacag tagacctgaa cctgaatatc gaatatggta
      241 agaacattct tgagacaagt aagaaagttc aggagaaagt aaaatcatcc atagagaata
      301 tgacaggtct tgaggttgca gatgttaata ttcatattgc cagtgtagat atggagaacg
      361 aaaaaggaaa gtaacccttt ccttttttgt gtacttaaga gatatttaga aaggaaagct
      421 gtttatacag caaaaattat gcgaagaaga gagcagagag aacatatttt taaattactg
      481 ttcatgacag aatttaattc agaggaagag atgagtgagc agctttcact ttattttgat
      541 actctggagg aactttcaga gaaagatcag gagtatatga gccggaaata ccgccatgta
      601 cttgaaaaat tagacgagat cgatgcactg ttaaatgaga caagcaatgg ctggaagaca
      661 aagagaatga gccgcgttga cctgactgcc ctgcgtctgg cagtgtatga actgaaatat
      721 gacaaagatg tacctacagg tgtagctatc aatgaggcgg tagagcttgc caaacgtttt
      781 ggcggtgaga cttccggttc ctttgtaaac ggtatccttg gcaagattgc caacagtgaa
      841 agcgaagaaa aatcagaatg aaaaacacat attctgtagg tcaggtaaac cgttatatca
      901 aaaatatgtt tacccaggat tatttactgc agaagattta tgtaaaaggc gaggtgtcca
      961 attgtaaata tcatacaagc ggacacatct atttttccct gaaagatgag acaggaacat
     1021 tgaattgtgt gatgtttgcc ggacacagac ggggacttgc atttgccatg aaaaatggcg
     1081 ataaggtgat cgttggcggg agtgtggatg tatatgaacg cgacggccgc tatcagatgt
     1141 atgcaaagga gattaccctg gaaggtgcag gaactctgta tgagagatat cttgcattga
     1201 agcaggaact ggaagatatg ggaatgtttg cacaggagta caaacagccc attccgagat
     1261 ttatcagacg tctgggtgtg gtaactgcac cgacaggtgc ggctgttcag gatatccgca
     1321 atatttccta tcgaagaaat ccctatttac agattatttt atatccggct cttgtacagg
     1381 gggcgggtgc ggcagaaagt attgtaaaag gaatacagat gcttgataag acagatgtgg
     1441 atgtgatcat tgtgggaaga ggcggcggct ctattgaaga tctctgggcg tttaatgaag
     1501 aaatcgtggc cagagctatt tttgagtgca gtacacccat tatttccgca gtaggtcatg
     1561 aaacggattt tacgattgct gatttcgttg ctgatctgcg agctcctact ccttctgctg
     1621 cggcagagct tgcggtggat gattacagaa gtgtgattga agcagtaagt atttacaggc
     1681 agcggttata tcgtgcaatg agtggtaaaa cggatctgta ccgcaacaga ctggagcatt
     1741 ttcagacaaa atttgcttat ctgagtccgg agaacagact gcgggagcag agacagcgtc
     1801 tggcagatct ggagaatgct gtacaaaatg gaatgaacag gaaacttcag gatgagagac
     1861 acagattgtc tgtttatctg gaacgttttg caggactttc ccctttaaag aaactgaatc
     1921 agggatattc ttatgtggca gataaatata aaaagacatt gacaagtgta gaacaggtac
     1981 aaaatgggga taccatttat atatctgtga cagacggtac gatagaagca gatgttacag
     2041 gtacggtgaa ggaggagaga atctatggcg aagaaagaaa actgtgagca gttacaggag
     2101 gagaaatctc tggaagagat gtttgcagaa ctggatctgc tggctgaaaa actggaaaac
     2161 agagacacat cgctggagga ctcttttata ctgtacagac agggcatgga acttttgaaa
     2221 ctttgcagcg ggaagctgga tacagtggag aagaaaatgc tgcagcttaa tgaggatggt
     2281 acatttagtg aattttcaag atgaattagc aaagagaacg gaagaaacag agaaagtgat
     2341 cagaagcttt ctgcctgcag aggcgggatt cgccggaaca atggctcagg ctatgaatta
     2401 cagtatgctg gcaggaggca agcgccttcg gccaatgttg atccgggaga catatcgcct
     2461 gtttgatggt aaggaagaag ttgtaaaacc gtttatggca ggaatggaga tgatccatac
     2521 tcattccctg atccatgacg atcttcctgc acttgatgat gatgattaca ggcgcggcag
     2581 actgactaca cataaggtat atggggaagc catgggggtg ttaagcggtg ttgcgctgct
     2641 taattacgca tacgaaacca tgtttcaggc atttgccctg gcaaaagagc aggatcgggt
     2701 aatccatgcg ttgagagtgg tctcacagaa aacaggcatt catggaatgc ttggaggaca
     2761 aagtgttgat gtggaaaatg acggaaagcc ccttgaaaaa gagatgcttg actatattta
     2821 cagaaataag acgtcagcat tgatcgaagc ctccatgatg acaggcgcaa ttcttgcagg
     2881 agcaaatgag caggaagtat ctgcagtgga gaaagctgcc ggaaatatag gactggcttt
     2941 ccagattcag gatgatattc tggatgtgac cagcactgca gaagaactgg gaaaacctgt
     3001 acacagtgat gagaagaata ataaagttac ttatgtgact ctttttggaa cagagaaggc
     3061 tgctgaacag gtggaagaac tctcagaaaa ggcaatcgat ctgctgaaga gtctgaataa
     3121 aaataacgag tttctgtatt tgttaattga aaaattaata aaccgcagaa aataaaggaa
     3181 ggatacaaat gattctggaa aagattaaga aacccaatga tatccataag ataccattgg
     3241 aagatttcga gcctctggca gcggagatca gggacttttt aatccggtca gtaagtcaga
     3301 caggcggaca tcttgcttca aatctgggag tagtggaact gactctggct cttcataatg
     3361 tgctggattt tccggaagac aaactaatct gggatgtagg acatcaggca tatacacata
     3421 agattcttac cggaagaaaa gatgaattta agaatctgcg acaggaaggt ggattaagcg
     3481 gttttcctaa gagaagtgag agcccctgtg atgcatatga tgcaggacac agttccaatt
     3541 caatttcggc aggactggga tatgtgcatg ccagagatat tctgggacag aagcatcatg
     3601 tggtttctgt gatcggagat ggtgctctca ccggaggaat ggcgtatgag gcattaaata
     3661 atgctgcaga gttaaagaca aattttatta ttatcataaa tgacaataat atgtccattt
     3721 ccagaaatgt aggaggcatg tccacttatc tgagtgcact gagaactgca gaa
//
LOCUS       DBMDXG010000194         3760 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5634_length_3760_cov_1.761684, whole genome shotgun sequence.
ACCESSION   DBMDXG010000194 DBMDXG010000000
VERSION     DBMDXG010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5634_length_3760_cov_1.761684"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="ACZPD3_06275"
     CDS             <1..288
                     /locus_tag="ACZPD3_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415638.1"
                     /translation="SAILSALASLQVIDDYIACKDARADRMAEKLYEKGRKDVKRCIR
                     IRKLIRRCMILSAIVTVVLVQYNKNEKLRESLEELLKMFRDGAVNESEEDF"
     gene            475..990
                     /gene="bilS"
                     /locus_tag="ACZPD3_06280"
     CDS             475..990
                     /gene="bilS"
                     /locus_tag="ACZPD3_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HIR1415639.1"
                     /translation="MGIQRYSIIYSSKTGNTKKLAEKIREVLPEENCDYFGTEGAKAL
                     SSDILYIGFWTDIGNADPVTLELLKSLKNKKIFLFGTAGFGGSEAYFQQVLGKVKESV
                     DESNTVMGEFMCQGKMPQSVRDRYVKMKEKPDHAPNLDELIQNFDRALSHPDNEDLDR
                     LEEIIRRDLKK"
     gene            1092..2156
                     /locus_tag="ACZPD3_06285"
     CDS             1092..2156
                     /locus_tag="ACZPD3_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIR1415640.1"
                     /translation="MTFYQELQLSSTGSKQLIRSTTDPKEKRRHILIYNFKVYLVMAF
                     CVAVVSMYSKLTGSSNSVVGVTVLLAVLVLRQADFGIRTTHGLLSIAGIFGILMAGPR
                     LANIVPPLAAFAVNAVCILLLMILGCHNVIMYNHSTFVLGYLLLLGYDVTGKEYTFRV
                     IGLLVGMVICMIVFYKNQRNRAYRRTFLDLFREFDLKSARSRWYVKLTLIVSSAMLFM
                     NLLGLPRAMWAGIACMSVCLPFTEDCIPRSVSRGMFNVVGCLLFIVLYLVLPKSMYPY
                     IGMIGGIGVGYSAGYPWQTAFNTFGALSIAAGIFGMPAAIALRIGANVLGAAYTVICN
                     KVTDKVAEYIGTNKCAENLS"
     gene            2274..>3760
                     /gene="cls"
                     /locus_tag="ACZPD3_06290"
     CDS             2274..>3760
                     /gene="cls"
                     /locus_tag="ACZPD3_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1415641.1"
                     /translation="MKQDTLEGRAKTKNGVKRLCFSAVCILLEAAFIIAMITKLNQYA
                     EIINLMTRLLAGVLVLKLYASDQTSSMKMPWVILILVFPILGVGLYLLIGLNGGTRKM
                     RERYDQIDRELLPLLPNDSECRETLGRKIPKAGNISDYIQKNASYPVYQNTDVIYYDE
                     AVKGLEAQLADLAKAEKFIFMEYHAIEDAQAWHKIQRVLEDRVKAGVEVRVFYDDMGS
                     IGFINTDFIKKMENVGIHCRVFNPFTPGLNVFLNNRDHRKITVIDGKVGFTGGYNLAN
                     EYFNFTHPYGQWKDTGIRLEGEAVRSLTVTFLEMWNAVSDKDKNDSDFTEFLVQTDYQ
                     AKQTGFIQPYADSPMDHEQVGEEVYISMVNKAEKYCWFMTPYLIITDEMTHALCLAAK
                     RGVDVRIITPGIPDKKMIYNITRSFYHGLVKHGVRIYEWTPGFCHAKMSVADDCMATC
                     GTINLDYRSLYHHFENGCFMADCQAVLDIRNDLAATMDECREVTE"
BASE COUNT         1156 a          653 c          946 g         1005 t
ORIGIN      
        1 tctgccatcc tgtcagctct ggcatctttg caggtgattg atgattatat tgcctgcaaa
       61 gatgccagag ctgacaggat ggcagagaaa ctctatgaaa agggaagaaa agatgtaaag
      121 agatgtatcc gcatccgaaa actgatccgc agatgtatga tcctgtctgc cattgttaca
      181 gtggtgctgg ttcagtataa taagaatgaa aagctgagag aaagtctgga agagctgctg
      241 aaaatgttcc gggatggagc cgtaaatgaa tcagaggaag atttttaaaa tgttttaccg
      301 aattcggcta cggtagaccg aatgtgatta tgtatcagtt ggaaggaata agcaatataa
      361 ataatgcaga caaaaaatgt attttcggaa tttttttaca aaaaatcttc ttataaaagc
      421 aaaaacaggg aagactccga gaatgcataa aacaaaaaag gagcaagaga cagcatggga
      481 atacaaagat actcgattat atacagcagt aaaacaggaa acacaaagaa gcttgcggag
      541 aagatcaggg aggtactgcc tgaggaaaac tgtgattatt ttggaacaga aggagcgaag
      601 gctctttcat ccgacatatt atacattgga ttctggacag atatagggaa tgcagatcct
      661 gtaacacttg aactgttaaa gagtctgaaa aacaagaaga tctttctgtt tggaacagca
      721 ggtttcggag gcagtgaagc ctatttccag caggttctcg ggaaagttaa agaatctgtg
      781 gatgagagta atacagtgat gggagaattt atgtgtcagg ggaaaatgcc ccagtcagtc
      841 agggacagat acgtaaagat gaaagagaag ccggatcatg caccaaacct ggatgaactg
      901 atccagaatt ttgacagggc gttatctcat cctgacaatg aggatctgga cagactggaa
      961 gaaataatca gaagagacct gaaaaaataa ttaagtcttg actaacagag ggaactgcgg
     1021 tatagtgctc gttactaaaa aaggtaattc ataaattttt atagattttc gcgaaagaga
     1081 ggtaacgaga tatgacattt tatcaggaat tacaattgag ttcgaccggc tccaagcagc
     1141 tgatcagaag caccacagac ccaaaagaga aaagacgaca cattttaatc tataatttta
     1201 aagtgtatct ggttatggct ttttgcgtag cagtggtgag catgtacagc aagcttacag
     1261 gaagcagcaa cagtgtagtt ggtgttactg ttctgctggc agtgcttgtg ctacggcagg
     1321 cagatttcgg gatcaggaca acacatggac tgctttccat tgcagggatc ttcggtattc
     1381 ttatggcagg accccgtctg gcaaatatag tgcctccgtt agccgcgttt gcagtgaatg
     1441 ctgtgtgtat tttactgctg atgatactgg gctgtcataa tgtgatcatg tacaatcatt
     1501 ctacctttgt tctgggctat ttgctgcttc tgggctatga tgtgacagga aaggaataca
     1561 ccttccgtgt gatcggcttg ttggtgggaa tggtgatctg tatgatcgtc ttctataaga
     1621 accagagaaa cagagcctat cgaagaacct tcctggattt gttccgggaa tttgatctta
     1681 agtcagcaag aagcagatgg tatgtgaaac tgacactgat cgtatccagc gcaatgctgt
     1741 ttatgaatct tctgggactt cccagagcaa tgtgggcagg aatcgcctgt atgtccgtat
     1801 gtctgccttt tacagaggac tgtattccaa ggtctgtgag cagaggaatg tttaatgttg
     1861 tgggctgtct gctgtttatt gtgctgtatc tggtacttcc taaatccatg tatccgtaca
     1921 ttggaatgat cggaggaatc ggagttggat attcagcagg atatccgtgg cagactgcat
     1981 ttaatacatt tggagcgttg tctattgcag cagggatctt cggaatgccg gcagcaatag
     2041 cacttcgtat aggtgccaat gttctgggag cagcttatac ggtaatctgc aataaagtaa
     2101 cagataaagt agcagagtat ataggcacaa ataaatgtgc agaaaatctt tcctgactga
     2161 aactgcggag cggttataag caagtaaaaa agcggatgat tttatccgtt tgatatgtgt
     2221 tctgggaata gagtgaatat taacgtacaa tgacagaggg agagagaaag aatatgaaac
     2281 aggataccct ggaaggcagg gcgaaaacga aaaatggtgt aaaacgatta tgcttttccg
     2341 cagtatgtat tttactggaa gcagctttta tcattgctat gatcacaaag ctgaatcaat
     2401 atgcggagat tatcaatctg atgacaaggc ttcttgcagg tgtactggtt cttaagctgt
     2461 atgcgtcaga ccagacttca tctatgaaaa tgccctgggt tattttgatc cttgtattcc
     2521 ctatcttggg agtgggattg tacttactga ttggtctcaa tggcggtacc cgtaaaatgc
     2581 gggagcgtta tgaccagatc gacagggaac ttctgccgtt gcttcccaat gacagtgagt
     2641 gcagggaaac gcttggcagg aaaatcccga aggcaggcaa tatttctgat tatatacaga
     2701 aaaatgcctc ctatccggtc tatcagaata cagatgttat ttattatgat gaggcagtca
     2761 aagggctgga ggctcagctg gcagatctgg caaaggcaga gaaattcatt tttatggaat
     2821 atcatgccat tgaggatgca caggcctggc ataaaatcca gagagttctg gaagaccgcg
     2881 taaaggcagg cgtagaagtc agagtatttt atgacgacat gggttccatt gggtttatca
     2941 atacagactt cattaagaaa atggaaaatg tgggaatcca ttgcagagtg ttcaaccctt
     3001 ttactccggg tctgaatgtg tttctgaata accgggatca ccgcaagatc acagtaattg
     3061 atggaaaagt aggatttacc ggcggttaca accttgctaa tgaatatttt aatttcacac
     3121 atccttatgg acagtggaag gacacaggta tccgtctgga gggggaggct gtacgttccc
     3181 tgacagtaac attcctggag atgtggaatg ctgtcagtga taaggataaa aatgacagtg
     3241 attttaccga atttctggtt cagacagatt atcaggcaaa gcagacagga tttatccaac
     3301 cctatgcgga cagtcctatg gatcatgaac aggtaggtga agaagtgtat atcagtatgg
     3361 tgaataaagc ggaaaaatat tgctggttta tgactccgta cctgatcatc acagatgaaa
     3421 tgacccatgc tctgtgtctt gcagccaagc gaggggtaga tgtaaggatc attacacctg
     3481 gtattccgga taaaaagatg atctacaata tcacacgttc cttttaccat ggactggtaa
     3541 aacatggagt gcgtatctac gaatggacac ctggtttctg tcatgcaaag atgagtgtgg
     3601 cagatgattg tatggcaacc tgcggaacca taaatctgga ttacagaagt ctgtatcatc
     3661 attttgaaaa cggctgcttt atggcagact gtcaggcagt tctggatatc cgaaatgatc
     3721 ttgcagcaac catggatgaa tgccgtgaag tgacagaaca
//
LOCUS       DBMDXG010000195         3734 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5678_length_3734_cov_1.395541, whole genome shotgun sequence.
ACCESSION   DBMDXG010000195 DBMDXG010000000
VERSION     DBMDXG010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5678_length_3734_cov_1.395541"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="ACZPD3_06295"
     CDS             complement(<1..856)
                     /locus_tag="ACZPD3_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1415642.1"
                     /translation="MSDQKLIIGYYRLSMEDDSEGESNSIINQRKLVKDYISHIPELA
                     AMPFQEFYDDGYSGSSMERPAIKQVLELARENKVQCIVVKDFSRFARNYIEMGTYLEQ
                     IFPFLGVRFISISDRYDSKDYKGKNSDIEVQFKGLIADFYVKDQSVKVKAAVSTRRGK
                     GEYCCGSAPYGYRINPENKRELVIVEDEAEVIRRVFELTNQRYSKMEICKLFNEEGVL
                     TPLQSMSRRQKSGSKKASSRGLQWTSDMIRKIVDDKTYIGCMVYGKTKIPDPGTGKEV
                     PVPRNHVLP"
     gene            complement(849..2504)
                     /locus_tag="ACZPD3_06300"
     CDS             complement(849..2504)
                     /locus_tag="ACZPD3_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1415643.1"
                     /translation="MARTAKRYKKNTEKKVLGIPVCMAAIYVRLSVDSDEKKSESIET
                     QVTLIKEFIQKHNENPNREYEIAVYNIYSDLGKTGTNFDRPGFERMMNDVREGKINCI
                     LVKDFSRFGRNYIETGNYLEKILPFMKVRFISVCDNYDSFAPDAKNQELSMNIKNLVN
                     DAYAKDISAKERAAKRIAQKNGEYVGSTAPYGYCVEKINGIYKLMVEPEAAKIVRRIF
                     EEYASGDGIQGIIDRLFEDRVHRISDYNQYHHVYCQDGENLHQWGNSSIRAVLNRNNY
                     YGDLVQRKYESRFQRGEKWCDILDESQWIITPNAHEPIISRELFEKAQVRLKAAQQKA
                     TKTTAGWEDDERAFYNVFYCGDCKRKMCTRRYRGNVYYFCNAAWYRDERKCSHKSISE
                     EKLQKIVRSELTRQFQLSGLRKKDMSVISSAVFLSKINEIQTEIRKLDADMERRSEKL
                     AQAFMKYKEGELSKEAYIEMKDDRNNWKEFCEERKKSLEQTIRKLEKQQKEEARFLRS
                     LLELDGTTRINAELAEGLIESMYLYGDGRLEINFGFKGAVEHE"
     gene            complement(2505..>3734)
                     /locus_tag="ACZPD3_06305"
     CDS             complement(2505..>3734)
                     /locus_tag="ACZPD3_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1415644.1"
                     /translation="ESYCRDISGKVRSHQKIKREKGEFIGAFAPYGYCKDPENKNCLV
                     IDSYAADIVRKIFSWKIDGFSLGAIAEKLNVRHVQSPKEYKKANGENYNSGFHSSDTP
                     KWSAVQIKRILTNEVYIGNMVQGKQERISYKVKQRLDKPESEWVKVENTHPAIIRQND
                     FDVVQKLLQYDGRASKTSDSANFFSGFVFCGDCKTPMIRRVNQYKGKKKAFYICQTKN
                     KGGDCTRHSISEEVLKRIVLKEIQAYTALFVDYQMIMEELCEMQVSYDQVIGYDTQIS
                     KLQEEYNRYYSLKASLGDDLKEGLISKEEFDDFRESYGRKCEELEQMIENQKKLVKQM
                     FEGGVSATVQLEDWKKSLEIKELDRTLLALTVDKIYIYENKQIKIHIRYQDMIEKMKV
                     IRRFYAEHRTECRKEVE"
BASE COUNT          940 a          939 c          557 g         1298 t
ORIGIN      
        1 gcggtagaac atgatttctc ggtaccggta cttctttccc agttccggga tctggaatct
       61 ttgtctttcc atagaccata cagcctatat aagtcttatc atctacaatc ttccgtatca
      121 tatcactcgt ccactgcaat cctcttgatg aagctttctt actgcctgat ttctgtcgtc
      181 tgctcataga ctgcaaggga gtcaatacac cctcttcatt gaataactta caaatctcca
      241 tcttggaata tcgctgattg gtcagttcaa atactctgcg gatcacttct gcttcgtcct
      301 ctacaatcac cagttctctc ttattttcag gattgattcg atacccataa ggtgcagagc
      361 cacagcaata ttcacctttt cctcgtcggg tgctgactgc tgcctttacc tttacagact
      421 ggtctttcac ataaaagtct gcgatcagtc ctttaaactg tacttcgatg tctgaattct
      481 ttcctttata atctttagaa tcatatcgat ctgagataga aatgaagcgt actccaagaa
      541 atggaaaaat ctgctctaga taagttccca tctcaatata gtttctggca aaacgggaaa
      601 aatcttttac cacaatacac tgaactttat tctctctggc aagttccaga acctgcttaa
      661 ttgccgggcg ctccatactg gaaccagaat atccgtcatc gtaaaactcc tgaaatggca
      721 tagcagcaag ctccggaata tgggaaatat aatcttttac cagttttctc tgattgataa
      781 tgctgttact ttctccctct gaatcatctt ccatggaaag acggtaatat ccaataatca
      841 gtttctgatc actcatgttc taccgccccc ttaaacccga agttgatttc cagtctgcca
      901 tcaccataca gatacatact ttcaatcaag ccctctgcaa gttccgcatt gattctggtt
      961 gtcccatcca gttccaacag acttcgtaaa aatctggctt cttctttctg ctgtttttcc
     1021 agctttcgta tggtctgctc cagagacttc tttctctctt cacaaaactc tttccagtta
     1081 ttacggtcat ctttcatttc tatataggct tctttggaaa gttcaccctc tttatatttc
     1141 ataaatgcct gtgccagttt ttctgaacgt ctttccatat ctgcatccag ttttctgatc
     1201 tcagtttgaa tctcattgat tttggaaaga aataccgcac tgcttataac agacatatcc
     1261 tttttccgca atccagataa ctgaaactgt ctggtcagct ccgaacggac aattttctgc
     1321 agcttctcct cggaaataga tttgtgacta cattttcttt catcccgata ccaggcagca
     1381 ttgcaaaagt aatacacatt gcctctgtat ctacgtgtac acattttccg cttacaatca
     1441 ccacaataga atacattgta aaatgctctt tcatcatctt cccaccctgc agtagttttt
     1501 gttgctttct gttgtgctgc ttttagcctg acctgtgctt tttcaaacaa ttctctgcta
     1561 ataattggct catgggcatt tggcgtaata atccactggc tctcgtccaa tatgtcacac
     1621 catttttcac ctctttgaaa tctggattca tattttctct gaaccagatc gccataataa
     1681 ttattccggt tcagcactgc acgtatcgaa gaatttcccc actgatgaag gttctctccg
     1741 tcctgacagt acacatgatg atattggtta taatctgaaa tccgatgtac cctatcctca
     1801 aacagcctgt caataatgcc ctgtatgcca tctcccgaag catattcttc aaaaatcctg
     1861 cggacaatct ttgcagcttc cggttccacc atcaacttat aaattccatt tatcttttcc
     1921 acacaatatc catatggagc tgtagatccc acatactcac cgtttttttg tgcaatacgt
     1981 ttcgctgccc gttcttttgc ggaaatgtct ttcgcataag catcattcac cagattcttg
     2041 atattcatgg ataattcctg attcttggca tccggtgcaa atgaatcata gttgtcacat
     2101 acagaaataa accgcacttt cataaaagga agaatctttt ccagatagtt gccagtttcg
     2161 atataatttc ttccaaatcg tgagaaatcc tttaccagaa tacagtttat tttaccttcc
     2221 ctgacatcat tcatcatccg ttcaaatccc ggtctgtcaa aatttgttcc ggtttttccc
     2281 agatcagaat aaatgttata tacagcaatt tcatactctc tgttcggatt ttcattgtgc
     2341 ttctgaatga actcttttat cagcgtaacc tgtgtttcaa tagattctga ctttttttca
     2401 tcactgtcta cggataatct gacataaatt gcagccatac ataccggaat cccaagaact
     2461 ttcttctctg tgtttttctt atatcttttt gctgtccttg ccatttattc cacctctttc
     2521 ctgcactctg tccggtgttc cgcataaaac cgtcttatga ctttcatttt ctcaatcata
     2581 tcctgataac ggatatgaat tttgatctgt ttgttttcat aaatatagat tttatctacg
     2641 gtcagtgcca acaatgtgcg atccagttct ttgatttcca gtgatttctt ccagtcctcc
     2701 aactgaacag ttgcagacac tccaccctca aacatttgct ttaccaattt tttctgattc
     2761 tcgatcatct gctccagttc ttcacatttt cttccgtaac tttcccgaaa atcatcgaac
     2821 tcttctttgc tgatcaatcc ctctttcaag tcatcaccta aagacgcttt cagactgtaa
     2881 tagcggttat attcttcctg caacttacta atctgcgtat cataaccgat tacctgatcg
     2941 tagctgactt gcatctcaca aagttcttcc ataatcatct gatagtctac aaaaagtgcc
     3001 gtatatgcct gaatctcttt caacacaatt cttttcagca cctcttccga aatactgtgt
     3061 ctggtgcaat ctccaccttt gttctttgtc tggcagatat aaaaggcttt tttcttcccc
     3121 ttatactgat ttaccctgcg tatcatcggt gtcttgcaat ctccgcaaaa cacaaatccc
     3181 gaaaaaaagt ttgcactgtc tgatgttttc gatgccctac catcatattg aagcagcttc
     3241 tgaaccacat caaaatcatt ctgcctgata attgccggat gcgtattttc tactttcacc
     3301 cactctgatt ctggcttatc caggcgttgc tttactttat agctgattcg ttcctgcttg
     3361 ccctgtacca tgtttccaat atagacttca ttggtcagaa tccttttgat ctgcactgcc
     3421 gaccatttcg gtgtatctga actatggaat ccggaattgt aattttcacc atttgccttt
     3481 ttatattctt ttggcgactg cacatgacgt acattcagtt tttctgcgat tgctccaaga
     3541 ctgaacccat caattttcca tgaaaatatt tttcttacaa tatccgctgc ataagaatca
     3601 atcaccagac agttcttatt ctccggatct ttgcagtaac catacggtgc aaatgctcca
     3661 ataaattcac ctttctcacg tttgattttc tggtggcttc gcactttacc ggaaatgtcc
     3721 cggcaatagc tttc
//
LOCUS       DBMDXG010000196         3703 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5724_length_3703_cov_2.025541, whole genome shotgun sequence.
ACCESSION   DBMDXG010000196 DBMDXG010000000
VERSION     DBMDXG010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5724_length_3703_cov_2.025541"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..416
                     /locus_tag="ACZPD3_06310"
     CDS             3..416
                     /locus_tag="ACZPD3_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVU_1555 family C-GCAxxG-C-C protein"
                     /protein_id="HIR1415645.1"
                     /translation="MMELGSMGYHCSQIIMIMTLETIGEENPQLVKAMGGLGGGIGYC
                     GDTCGCLTGSACAIGYFLGNLAPEEKEDAQMKPAVQELYQWFRQKTEEEFGAFYCKDI
                     THLDWGVIMEKCPGLIADTYTKVMEILTEREVLEL"
     gene            413..1738
                     /gene="trsS"
                     /locus_tag="ACZPD3_06315"
     CDS             413..1738
                     /gene="trsS"
                     /locus_tag="ACZPD3_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM (seleno)protein TrsS"
                     /protein_id="HIR1415646.1"
                     /translation="MITIGKTKSVCPECMKVVPAIKGIGEDGIYLVKECNAHGKFQTL
                     IWEGNAADYLSWGRENLSAETPVNPKVKEKSCPDNCGLCEEHERKGCCMILEVTKRCN
                     MHCPVCFASAGEDREKGDIPICEIEKQYDFLMAHGGPFNIQLSGGEPTMRDDLPEIIH
                     MGREKGFTFFQLNTNGIRLAREEGYAGKLKKAGLNTVFLQFDGVTDQVYETLRGQAMM
                     ELKKKAVLNCSEAELGIALVPVIAPGVNDMQVGNILKFGLDHMPFVRGVHFQPISYFG
                     RCSQKRPQNPITIPKMLRLIEEQTEGLMKIEDFAGGGAENPYCSFHASYLRKGERELK
                     LLEKKSGKGCCCTTSDDSRQYVENQWSYSTKTYDEGEMTQTDALDEFLIRIHNETFAV
                     SGMIFQDAWNLDLDRLKRCYICEVDPDHGMVPFCAYNLTNLKGIYLYRK"
     gene            1759..2931
                     /locus_tag="ACZPD3_06320"
     CDS             1759..2931
                     /locus_tag="ACZPD3_06320"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVU_1553 family AMP-dependent CoA ligase"
                     /protein_id="HIR1415647.1"
                     /translation="MICRQEKITDITRETINKIQLDKLNAVLKREKERQGFYRDLPER
                     LESIDDLKTLPFTTESDLAQKGGRMLLCSQGEIQRIISEQTSGTTGAGKRVFYTEGDC
                     EHTIELFMAGLGEFIYPGSRTMVAMPFSGPSGLGELIAEAIRRIGAHPLLTGNNKTYG
                     ELKTILEEERPDTYVGMPTALLSMLRMCGKGSIKRALISGDACPETVMKAIEKILGTP
                     LWPHYGSREMGLGGAICCQAHEGMHMRENHCITEIIDKEGNVLPDGQWGELVITTIGM
                     EAQPMIRYRTGDHTRIIPGKCICGSEVRRLDFVRRIDQSKSMREMDELLFQFPELVDY
                     CVRSVGGKKEITALFISDNGEELIRNVCAEKNIISLDCRKAEWSDRALYPAKRIIL"
     gene            complement(3007..>3703)
                     /locus_tag="ACZPD3_06325"
     CDS             complement(3007..>3703)
                     /locus_tag="ACZPD3_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HIR1415648.1"
                     /translation="HFSGCLICGAPVTYSVESSVKTCSICHKEQLTNAVCENGHFVCD
                     ACHSYGTYIPVSTALRSSTEKDPLLLLEEIMDLPSVHMHGPEHHAIVPSVLLTALRNN
                     GERMNYDTALSEICKRAKQVPGGTCGYWGVCGAAAGAGIFMSVMTGSGPLHKDAWPFP
                     QKLVSVILSKLADVGGPRCCKRTSRIAIEEAIRFYRQFSSVKIPLSSVLCKYFEDNKE
                     CIREDCPYYPVNK"
BASE COUNT         1148 a          688 c          950 g          917 t
ORIGIN      
        1 gaataatgga attaggcagc atgggatatc attgttcaca gatcattatg atcatgactc
       61 tggaaaccat aggagaggaa aatccacagc ttgtaaaggc aatgggaggc cttggcggag
      121 gaatcggtta ctgtggagat acctgcggat gtctgacagg aagtgcctgt gccatcggat
      181 attttctcgg aaatctggct cctgaggaga aggaagatgc acagatgaaa ccggctgtac
      241 aggaattata tcagtggttc cgccagaaga cagaggaaga attcggagct ttttactgta
      301 aggatatcac tcatctggac tggggtgtga tcatggagaa atgtcctgga cttattgcag
      361 atacttatac aaaagttatg gaaattctga cagagcggga ggtactggag ctttgatcac
      421 catcggcaaa acaaagagtg tctgtccgga atgtatgaag gtagttcctg ccataaaagg
      481 tatcggtgaa gacgggatct atctggtaaa ggaatgcaat gctcatggaa aatttcagac
      541 cctgatctgg gaaggaaatg cagcagatta tctttcctgg ggaagagaaa atctctcagc
      601 agaaacgcct gtaaatccga aggttaaaga aaaatcctgt ccggataatt gcggactctg
      661 tgaggaacat gaaagaaaag gctgctgtat gatcctggaa gttacaaaac gctgtaatat
      721 gcattgtcct gtctgctttg cttcagcagg agaagacagg gaaaagggag atattcccat
      781 ctgtgaaata gaaaaacagt atgattttct gatggctcac ggaggtccct ttaatataca
      841 gctttcagga ggagaaccaa ccatgaggga cgatctgcct gaaatcatac atatgggaag
      901 agaaaaagga tttacatttt ttcagttaaa taccaatggg atccggcttg ccagggagga
      961 ggggtatgcc ggaaagctga aaaaagcagg actgaataca gtatttcttc agtttgacgg
     1021 tgtgacagat caggtttatg agactttacg tggacaggct atgatggaac tgaagaaaaa
     1081 ggcggtctta aactgctcag aagcagagct tggtattgct cttgtgccgg tcattgcgcc
     1141 gggagtaaat gatatgcagg tagggaatat tctgaaattt ggtctggatc atatgccctt
     1201 tgttcgtggc gtacattttc agcctatcag ttattttggc agatgttccc agaagcgccc
     1261 ccaaaatccc atcaccatac caaagatgct gagacttatt gaagaacaga cagagggact
     1321 gatgaaaatt gaggattttg caggcggcgg cgcagagaat ccctactgct cctttcatgc
     1381 aagttatctg agaaaaggag agcgggaact gaagcttctt gaaaaaaaat caggaaaagg
     1441 atgctgctgt actaccagtg atgattccag gcagtatgtg gaaaatcagt ggagctacag
     1501 tacaaaaacc tatgatgagg gagaaatgac acagacagat gcgctagatg agtttctgat
     1561 ccggatacat aatgaaacct ttgcagtatc aggtatgatc tttcaggatg cctggaatct
     1621 ggatcttgac cgtctgaaac gctgttatat ctgtgaagtg gatcctgatc acggaatggt
     1681 accgttctgt gcatataatc tgactaatct gaaaggaata tatttataca gaaagtgaaa
     1741 cgatccaata tagatgcaat gatctgtagg caggaaaaaa ttacagatat tacaagggaa
     1801 acaataaaca aaatacagtt ggataaactg aatgctgttc tgaaaagaga aaaagaaagg
     1861 cagggatttt atagagatct gccggaaagg ctggaaagca ttgatgatct gaaaaccctt
     1921 ccctttacaa cggaatccga ccttgctcag aaagggggca ggatgttact gtgctctcag
     1981 ggagaaatac agaggatcat ttcagaacag acaagcggaa caacaggtgc aggtaaacgt
     2041 gtattttata cagaaggaga ttgtgaacat accatagaac tttttatggc cggacttggt
     2101 gagtttattt atccgggaag caggactatg gttgccatgc cgttttcagg tccttctgga
     2161 ctgggagagc tgatcgcaga agcaatcaga cgtataggag cacaccccct tttaaccggg
     2221 aacaataaaa cctatggtga gttaaaaaca attctggaag aggagcggcc ggatacttat
     2281 gtaggtatgc cgacagcact gctttccatg ctcagaatgt gtggaaaagg cagtataaag
     2341 agagcactga tatcagggga tgcctgtccg gaaacagtaa tgaaagcaat agaaaaaatt
     2401 ttgggaacac cactctggcc tcattatggt tccagagaga tggggcttgg gggtgcgatt
     2461 tgctgtcagg ctcatgaggg catgcacatg agggagaatc attgtatcac agagatcata
     2521 gataaagagg gaaatgttct gcctgacggt caatggggag aactggtgat cacaaccatt
     2581 gggatggagg cacagcctat gatcagatac aggaccggcg atcatacacg tatcatcccc
     2641 ggaaaatgta tctgcggaag tgaggtaagg cgtctggatt ttgtaaggcg catcgaccag
     2701 tccaaaagta tgagagaaat ggatgaatta ctgtttcagt ttccggaact ggtagattac
     2761 tgtgtaagga gtgttggagg gaaaaaagaa atcacagcac tttttatatc agataacgga
     2821 gaagaactga tcagaaatgt ctgcgcagag aagaatatta tttctttgga ctgcagaaaa
     2881 gcagaatgga gcgatagggc attgtatcct gcgaaacgta ttattttata gagaaactat
     2941 tagaaaacag aacgaaaccc attgactatc agtcggattt cgttctgttt tttattttat
     3001 gaatagttat ttgttcacag gataatacgg acagtcctct ctgatacatt ctttattatc
     3061 ttcaaaatat ttacatagta cagaagacag cggaatcttt acagagctga actgcctgta
     3121 aaacctgatc gcttcttcaa tagcaattct gctggtgcgt ttacagcagc gcggaccacc
     3181 aacatcagcc agtttggata aaataacaga gacaagcttc tgaggaaagg gccatgcatc
     3241 cttatggagg ggaccggatc cggtcataac ggacataaag attccggcac ctgctgctgc
     3301 accacacact ccccagtaac cgcaggtacc accgggaacc tgcttcgctc ttttacagat
     3361 ttcagataat gcggtatcgt aattcatacg ctctccatta ttgcgaagtg cagtcagaag
     3421 aacactggga acaatggcat gatgttcagg tccatgcatg tggacagaag gaaggtccat
     3481 gatctcttcc agaagcagca ggggatcctt ctctgtgctg cttctgaggg ctgtgctcac
     3541 cggtatataa gtaccatagc tgtgacaggc atcacagaca aaatggccat tttcacagac
     3601 agcatttgtc agctgctctt tgtggcagat gctgcaggtt ttcacagaac tttcgacgct
     3661 gtatgtgacc ggtgctccac agatcaggca gcctgaaaaa tgt
//
LOCUS       DBMDXG010000197         3703 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5725_length_3703_cov_1.683509, whole genome shotgun sequence.
ACCESSION   DBMDXG010000197 DBMDXG010000000
VERSION     DBMDXG010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5725_length_3703_cov_1.683509"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(26..526)
                     /locus_tag="ACZPD3_06330"
     CDS             complement(26..526)
                     /locus_tag="ACZPD3_06330"
                     /inference="COORDINATES: protein motif:HMM:NF040162.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HIR1415649.1"
                     /translation="MLALKITDVKDFMNKLLIGEVFDRFSLVEASVTTFNTFTINGKL
                     HYDFFDTDTKAAFEENSTEYSLWHDVKPYCFSIIRGRRTPLNFRIVFELSHDQTQSLL
                     KNEQHIADIPVQSFYLNIRYKNQSLLCTTGVSYTSFSPDKRLEHLWDDSMTVFLSSHH
                     IPCEKL"
     gene            781..1620
                     /locus_tag="ACZPD3_06335"
     CDS             781..1620
                     /locus_tag="ACZPD3_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1415650.1"
                     /translation="MRYKIIIDSCGELLDEWKKDECFESIPLTLMVGAEQIIDDETFD
                     QAEFINKVAACPECPKSACPSPERYMRAYDCEAEHIYAVTLSSELSGSYNSALLGRDL
                     IMEDHPDKKIHVFNSRSASIGESLIGMKIQECEEAGMSFEEVVSTVEHYIEGQHTFFI
                     LENLDTLRKNGRLSKVKALVASALKIKPVMGSTDDGNICQLDQARGMNRALIKLVEQV
                     IEKTPDSAEKVLAISHCNCPARAQVLKEAFEERMKLAKIVVLDTAGVSSMYANDGGVI
                     VAV"
     gene            1695..2168
                     /locus_tag="ACZPD3_06340"
     CDS             1695..2168
                     /locus_tag="ACZPD3_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415651.1"
                     /translation="MSQKKVDAYKARKGLHNKTDRKEKVLFGLEMFAWAFICVVIVAW
                     IGYSAYVKVTGAKENVVQNTVMDTTALDNYISNLSTDASDGTDSTDADTAEEEDTTAS
                     TDTDSEDADAADTTADDSETETADKSEEADDTNVADDKAAETADSTTDADSADAK"
     gene            complement(2301..2501)
                     /locus_tag="ACZPD3_06345"
     CDS             complement(2301..2501)
                     /locus_tag="ACZPD3_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR1415652.1"
                     /translation="MNKGTVKWFNAEKGYGFITGEDGADVFVHFSAINGEGFKSLEEG
                     QAVTYDLTEGARGMQAANVTKL"
     gene            2776..>3703
                     /locus_tag="ACZPD3_06350"
     CDS             2776..>3703
                     /locus_tag="ACZPD3_06350"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR1415653.1"
                     /translation="MGILIQGGRIVDAATDTDKKGDIYLEDGVITEIGEKLKIKDKSD
                     KVIDAKGCLVMPGLIDLHVHFRDPGQTQKEDIETGSRAAARGGVTTVVAMPNTTPVID
                     SPDRVNYVHNKAKQLAGIHVLQAGAITQGEKGQELSDIEGMVKAGIPALSEDGKSVMN
                     TRLCKEAMEVAEKFNVPIFAHCEDIDLRGDGCMNEDENARRLGLPGICNAVEDVIAAR
                     DILLARETGARLHLCHCSTEGVAKMMEIVKEEGLDNITAEVCPHHFILTSDDIKCDDP
                     NYKMNPPLRTKKDVDALIRGLKDGSFKVISTDH"
BASE COUNT         1232 a          632 c          859 g          980 t
ORIGIN      
        1 gataaagact gataacagaa tctactcata atttctcaca gggaatgtga tgactggaga
       61 gaaaaacagt catggaatca tcccagagat gttccagacg tttatccgga ctgaatgaag
      121 tataagaaac acctgttgta cataaaaggg actggttctt gtaccggata ttcagataaa
      181 agctttgcac aggaatatct gcaatatgct gttcattttt taacaacgac tgtgtctgat
      241 catgagaaag ttcaaataca atcctgaaat tcaaaggtgt gcgtctgccg cgtatgatag
      301 aaaaacaata aggttttaca tcatgccaca gggaatattc tgtggaattc tcctcaaaag
      361 cagcctttgt gtcagtatca aagaaatcgt aatgaagttt tccgtttatg gtaaaagtgt
      421 tgaaggttgt cacagatgcc tcgacaagag aaaaacggtc aaatacctca ccgatcagga
      481 gtttattcat aaaatctttt acatcagtaa tttttaaagc aagcatacaa cacctctctg
      541 ataacatgat ttacagctgt tcagaattaa aaaaagactt ctggattgct gcatatttct
      601 gaacatattt tgtggatcag gcagcgcttc ctgcactttg cgggaagcgt ctttttatta
      661 taaagcactt ttgaaaaaaa gaacagtgtt tttattaaaa agtcttttca aacgtaaagc
      721 ggtgtgataa gatatatctg gtattgaata ctacatataa tggaaaagag gacgatataa
      781 ttgagatata agattatcat agatagttgt ggagaacttt tggacgaatg gaagaaagat
      841 gaatgttttg aatccattcc actgacgctg atggtaggag ctgaacagat catagatgat
      901 gaaacatttg atcaggcaga gtttattaat aaagtggcag cctgcccgga atgtccgaaa
      961 tcagcctgcc cgtcacctga gagatatatg agagcatatg actgcgaggc tgagcatatt
     1021 tatgcagtga ctttgtcttc tgagctgagt ggttcctata acagtgcgtt acttggcaga
     1081 gatcttatta tggaagacca tccggataaa aaaatacatg tgtttaattc acgttccgca
     1141 tccatcgggg aaagcctgat cggaatgaag atacaggaat gcgaagaagc agggatgagt
     1201 tttgaagaag ttgtttcaac tgtggaacat tatattgaag gacagcatac attttttata
     1261 cttgagaatc ttgatacttt gagaaagaac ggccgtctga gcaaagtgaa agcgttagtg
     1321 gccagtgcat tgaaaatcaa accggtcatg ggttctacgg atgatggaaa tatctgccag
     1381 ctggatcagg caagaggcat gaatcgtgct ctgatcaagc ttgtagagca ggtaatcgag
     1441 aaaactccgg acagtgcaga gaaggttctt gccatcagtc actgcaattg tccggcaaga
     1501 gcacaggtgc tcaaagaggc ttttgaagag agaatgaaac ttgcaaagat cgtagtcctt
     1561 gatacagcgg gtgtaagttc tatgtatgca aatgacggcg gagtgatcgt tgcagtataa
     1621 actaattgtt ttcttttgca gtaaagtgtg ctataatcag aaaatacgaa atattgatgc
     1681 aaaggagatc tatcatgagc cagaagaaag tcgatgctta taaggccaga aaaggactgc
     1741 ataacaaaac agacagaaaa gaaaaagtac tttttggact ggaaatgttt gcctgggcat
     1801 tcatttgtgt tgtgattgtt gcatggattg gttattctgc atatgtaaaa gtgacaggag
     1861 ccaaagagaa tgtggttcag aatacagtaa tggatactac tgcacttgac aattacattt
     1921 cgaatctgtc aacagatgca tccgatggta cagacagcac tgatgcagat acagcggagg
     1981 aagaagatac aacagcttcc actgatacag acagtgaaga tgctgatgca gcagacacaa
     2041 ccgcagatga ttctgaaacg gaaaccgcag ataaatctga agaagcagat gatacaaatg
     2101 tagcagacga taaagctgca gaaacagcag attcaacaac ggatgcagac agcgcagatg
     2161 ctaaatagaa attgaatctc tgatggattc acagaagaaa agcaataaaa aatccccgga
     2221 taatccgggg atttttttga ggtttaatcc gtatagaaaa gtgcggaatt caatcctctc
     2281 acgttaacaa aaattataaa ttataatttt gtaacgtttg cagcctgcat accacgagca
     2341 ccttctgtta agtcgtatgt tacagcctgt ccttcttcaa gggatttgaa tccttcacca
     2401 ttgatagcgg agaaatgtac aaatacgtct gcaccgtctt caccagtgat aaatccgtaa
     2461 cctttttctg cgttgaacca ttttacagtt cccttgttca tgttgttacc tccataaaaa
     2521 ataaaaaaat tatatagttt ctggcaaata aaaaaatcac cagattttac agattatata
     2581 ctgttatata atctctaaaa accagtgact atacattaaa taatccagat atgaactgat
     2641 tatatctccc gcattttgaa ttgtcaagca aaaaagactg cacatcagta aaaaaaggct
     2701 ttataaaaga agaagtttcg tgtattatgt atatcagaaa gttattgtca aacgacaacg
     2761 aagggagaga attgcatggg tattctgatt cagggaggac gtatcgtaga tgcggctact
     2821 gacactgata aaaaaggcga tatttatctg gaagatggcg tgatcaccga gatcggtgag
     2881 aaacttaaaa taaaagataa aagcgacaaa gtaatagatg cgaagggatg tctggtaatg
     2941 ccgggactga tcgatcttca cgttcatttc agagatccgg gacagacaca gaaagaggat
     3001 atagagacag gttcccgggc tgctgcaaga ggtggtgtga caacggttgt tgcaatgccg
     3061 aatacaacac cggtaattga cagtccggat cgtgtaaatt atgtgcataa taaggcaaaa
     3121 cagcttgccg gcattcatgt gctccaagca ggtgccatta cgcaggggga gaagggacag
     3181 gaactttctg atatcgaagg aatggtaaaa gcgggaatcc cggcattatc tgaagacggc
     3241 aaatctgtta tgaataccag actttgcaaa gaagctatgg aagtggcaga gaaattcaat
     3301 gttccgattt ttgctcactg tgaggatatt gatctgcgcg gtgatggatg tatgaatgag
     3361 gatgagaatg cgcggagact tggtctgcca ggtatctgca atgcagttga ggatgtgatc
     3421 gcagcgagag atattcttct tgccagagag acaggggcaa gactgcatct gtgccattgt
     3481 tccacagaag gtgttgcgaa gatgatggag attgtgaagg aagagggatt ggacaatatc
     3541 acagcagagg tttgcccgca tcattttatc ctcacttccg atgatattaa atgtgatgat
     3601 ccaaactata agatgaaccc tcctcttcgt acgaagaaag atgtagatgc actgatccga
     3661 ggactgaagg atggttcctt taaagtgatc agtacagatc atg
//
LOCUS       DBMDXG010000198         3681 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5778_length_3681_cov_2.152514, whole genome shotgun sequence.
ACCESSION   DBMDXG010000198 DBMDXG010000000
VERSION     DBMDXG010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5778_length_3681_cov_2.152514"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /locus_tag="ACZPD3_06355"
     CDS             complement(<1..2191)
                     /locus_tag="ACZPD3_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415654.1"
                     /translation="MHLRGNVIYNTWENFWKKAYKSSYFRAQYDETSERTDWSLSERQ
                     LFTQSNGRTLLGQDTMGRLHFLCTPHDKIYAVPSGGTDLGGQFKGYIGQYYQNDTALL
                     LGKMKYDLELDNGLMISGANKQSWTTYYDDFLPVTATEHPELETRIFSFAPILEKEAV
                     PASSIHPVPGPAGAFYCIEVKNISGCKMKGKLRLSFDQKFVNQFEHYGKYFDDYTVTP
                     YKSEWDQKLLVLWHPEACAAIQLLDSVCEGQGDNPRIYVPFELKANESRTFTTVIALT
                     PKREEIYSALGTLYQHTPLEWLNVTDDFWKERFGKITTDIRENQEAGEKYRDMQVRFI
                     LDNFNCLSFREDGSFLTNWQGAPSHSLSRLWGIDITPDVVSVMYAVPEVGKSAMFYLA
                     ERNRPRYSLYSDHSMFYYISLLVIAGKYLELTGDEKFFRDHPELVAAIDEIYDGMMKH
                     KHKEKALISSRYASDLIVFRKYDYGANVQCYYALKSYRRILKLLERDATDVDRFMEQM
                     KADMKELMEGSGPFGRQITGGNNLGENEERFYIQDDLNYYGGEDTATVMAPLYGLYEF
                     DYEPYVNLHRYARSMYITNYDPEFQTMRELHFGMNPSATGCTLKLGGSLTRQEMLDNL
                     NLLYERLDETGSLFWWPRATNKKRCLTRCSQGQGAWIQQSIEQWFGLRMDGTQKTLVI
                     KPQGLLSGYKLQKTHLGAYVFDIEYREEKGKTVINIKNYNEEAIKVIF"
     gene            complement(2209..2937)
                     /locus_tag="ACZPD3_06360"
     CDS             complement(2209..2937)
                     /locus_tag="ACZPD3_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415655.1"
                     /translation="MNTVEVFMMPPRWIPSKFMWENYQEVFDTLPFGKYFLNSFIVTG
                     GCVVGTMLTSSICAYGLARIKWRGRNVVFACIISSMMLPVAVTLIPTFLMWRTIGITD
                     SFIPLIVPAWFGGGAFYIFLLRQFYLGIPKDFDEAAYLDGASHIQIFTKIILPITKPA
                     LAVVGMFAFLNSWNDFLSPLVYLNSEKKYTVALGLQLFTGSYRGEWNLMMAAACLVLA
                     PVVVVFAIGQKYLVEGVTMSGVKG"
     gene            complement(3048..>3681)
                     /locus_tag="ACZPD3_06365"
     CDS             complement(3048..>3681)
                     /locus_tag="ACZPD3_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415656.1"
                     /translation="IIFSFFIAMLLNTNVKGKGFFRTIFYLPTVVPIVAMAAIWMWIF
                     NPDMGLANNILKAMHLPVSTWLSGESSVIPTLVFINLWTTGSTMVIFLAGMQDVPRQL
                     LEAVEIDGGGFWAKLLHVTIPMMTPTIFYNVVMGIINGFQIFTQSYVMTQGGPNNSSL
                     FYVYYLYREAFEFGRMGNACAVAWVLFLIIMVLTAVIFKFSNRWVYYGGE"
BASE COUNT         1106 a          852 c          609 g         1114 t
ORIGIN      
        1 caaaaataac ctttattgct tcctcattat agtttttaat attaataaca gtttttcctt
       61 tttcttctct atattcaata tcaaaaacat aagctccaag atgtgttttc tgaagtttat
      121 aaccggaaag caatccctgt ggcttgatga ccagcgtttt ctgtgttcca tccatcctga
      181 gaccaaacca ctgctcaata ctctgctgaa tccatgcccc ctgtccctgg ctgcatctag
      241 tcaggcatct tttcttattt gttgctcttg gccaccagaa aagtgagcct gtttcatcca
      301 gacgttcgta aaggagattc aggttatcaa gcatttcctg tctggtaaga cttccgccaa
      361 gttttaaagt acagcctgtt gcagatggat tcatgccaaa atgtaattca cgcattgtct
      421 ggaattccgg atcatagttt gtgatataca tagaacgtgc atatctatga agatttacat
      481 atggctcata atcgaactca tatagcccat acaaaggtgc cataactgta gcagtatcct
      541 caccaccata ataattcaga tcatcctgga tatagaagcg ttcctcattc tcacctaagt
      601 tgtttccgcc tgtaatctgt ctgccgaatg gtccgctacc ttccatcagt tctttcatgt
      661 ctgctttcat ctgttccata aatctgtcca catctgtcgc atcccgttcc agaagtttca
      721 gaattcttct gtaagatttc aacgcataat aacactgtac atttgcaccg taatcatact
      781 tgcggaatac aataagatcg ctggcgtatc tgctagaaat cagtgctttt tctttatgtt
      841 tatgcttcat cattccatcg tagatctcat caatagcagc aaccagttcc ggatgatctc
      901 tgaagaattt ctcatctcct gtcagctcca gatattttcc tgctattaca agcagagaaa
      961 tatagtaaaa catactatga tccgaataca ggctataacg tggccggttt ctttctgcca
     1021 gataaaacat tgcacttttt cctacctcag gtactgcata cataacactt accacatcag
     1081 gagtaatatc aattccccag agcctcgaaa gactatggga cggtgccccc tgccagttgg
     1141 tcagaaaact tccatcttct ctgaaagaaa ggcagttaaa attatcaaga ataaaccgta
     1201 cctgcatatc acggtatttc tcaccagcct cctggttttc ccgaatatct gtagtgattt
     1261 ttccaaatct ttctttccag aaatcatcag taacattcaa ccattccagc ggtgtatgtt
     1321 gatacagtgt tccaagggca gaataaatct cttctctctt tggagtaaga gcaattactg
     1381 ttgtaaatgt tctgctttca tttgctttta attcaaatgg tacataaatt ctaggattat
     1441 ctccctggcc ttcacatacg gaatccagaa gctgaattgc tgcacaggct tccgggtgcc
     1501 ataataccag caatttctga tcccattctg atttatatgg tgttactgta taatcatcga
     1561 aatatttccc ataatgctca aactgattta caaacttctg gtcaaatgac agacgaagtt
     1621 ttcctttcat tttgcatccg gaaatatttt ttacttctat acagtagaat gctcctgcag
     1681 gtcccggaac cggatggatt gatgatgccg gaactgcttc tttctccaga atcggcgcaa
     1741 aagagaatat tctggtttcc agttccggat gctctgtagc agtaaccgga agaaaatcat
     1801 cataataagt tgtccatgac tgcttatttg cacctgagat catcagacca ttgtccagct
     1861 ccagatcata tttcattttt ccaagaagta atgctgtatc attctgataa tactgaccta
     1921 tgtatccttt aaactgtcct cccagatctg ttcctccgga tggtactgca taaattttat
     1981 catgaggagt gcacaaaaag tgcagtctgc ccatggtatc ctggcccaaa agtgttcttc
     2041 catttgactg tgtaaatagc tgtctctcac ttaatgacca gtctgtgcgc tccgaagttt
     2101 catcatactg tgctctgaaa taagaagact tatatgcctt tttccagaaa ttttcccatg
     2161 tattataaat cacatttcct cttaaatgca ttgtttttcc tcctattctt agcctttcac
     2221 accagacatt gttacaccct caacaagata cttctgacca atggcaaata caaccacaac
     2281 aggtgcaagc accaggcagg ctgctgccat cattaaattc cattcacctc tgtaagaacc
     2341 agtaaacaac tgaagtccca gtgcaactgt atattttttc tcagaattta aatatacaag
     2401 cggactcagg aaatcattcc aggaatttaa aaacgcaaac atacctacaa cagccagggc
     2461 cggttttgta attggcagga taatttttgt aaaaatctga atatgggaag caccgtccag
     2521 atatgctgcc tcatcaaaat ccttcgggat tcccagataa aactgacgaa gcaggaatat
     2581 gtaaaatgca ccgcctccaa accatgccgg aacaatcagt ggaataaagc tgtctgttat
     2641 tccaatagtt ctccacatca gaaatgtagg aatcaaagta accgcaactg gaagcatcat
     2701 actgctgatg atgcaggcaa ataccacatt tctgcctctc catttaattc ttgccaatcc
     2761 atatgcacag atggaactgg taagcattgt tcccactaca catcctccgg ttacaataaa
     2821 tgaattcagg aaatattttc caaagggaag tgtatcaaaa acttcctgat agttttccca
     2881 cataaatttg gaaggaatcc agcgaggcgg catcataaac acttccactg tgttcatcag
     2941 tgaacttctg atcatccagt acaaaggaac cagacaaaac aaaacaccta aaatcagcgc
     3001 aaagtatatt agaatttttg aaataatttt ctttgtattc ttcatattca ttctcctcca
     3061 taataaaccc aacggttgga aaacttaaat atgactgcag tcagtaccat aatgatcagg
     3121 aacaataccc aggcaactgc acaggcattt cccattcttc caaactcaaa tgcttctctg
     3181 tacagatagt atacatagaa caaactggaa ttgtttggtc caccctgtgt cataacataa
     3241 gactgggtaa atatctggaa tccattgatg attcccatta ccacattata aaaaattgta
     3301 ggagtcatca taggaatcgt aacatgaagc agctttgccc agaatccgcc tccatcaatc
     3361 tcaactgctt ccagaagctg tcttggtaca tcctgcattc cggcaaggaa aattaccatt
     3421 gttgatccgg tagtccacag attaataaaa accagtgtgg gaataactga actttcacct
     3481 gaaagccatg tacttaccgg taaatgcatt gcctttaaaa tattgtttgc caatcccatg
     3541 tccggattga aaatccacat ccaaatcgct gccattgcta cgatcggaac tacagtcgga
     3601 agataaaaga ttgttctgaa aaatcctttt ccttttacat ttgtatttaa cagcattgct
     3661 ataaaaaaag aaaatatgat a
//
LOCUS       DBMDXG010000199         3668 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5805_length_3668_cov_2.430053, whole genome shotgun sequence.
ACCESSION   DBMDXG010000199 DBMDXG010000000
VERSION     DBMDXG010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5805_length_3668_cov_2.430053"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(33..905)
                     /locus_tag="ACZPD3_06370"
     CDS             complement(33..905)
                     /locus_tag="ACZPD3_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1415657.1"
                     /translation="MAKKRKKPKMEFRYYQMPAGSPILALLGQKWVQNYGNDVDYLHF
                     HNYLEIGFCYEGDGVMAFGEAKMRFSGREFTVIPPNYAHTTNSDLGTVSKWEYLFIDV
                     EGFMKKFLNNPVKAEKMIQRIYSKALFLKENESPSIAAKILKIMNIMREGEEFYIEES
                     SGVLAALLVEIARLNRVSPEDRVEEETGKLTNMITRVLDFVSYHYMEDIRIEDLAKIC
                     HISETHFRRVFTSHMKVSPLEYINSVRIHTACEFLQKTDIPVADIAHKCGFTTNSTFN
                     RNFRQIMGVTPVER"
     gene            complement(1150..2052)
                     /locus_tag="ACZPD3_06375"
     CDS             complement(1150..2052)
                     /locus_tag="ACZPD3_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1415658.1"
                     /translation="MSNKKNSDNKSNKKKNTKNMKINEWPRHGIQALWAFITNSHVTG
                     FVTGKIYTGKLKNACVPGLNCYSCPGAVGACPIGSLQAVIGSWNFKMAYYVVGFLIFI
                     GAMVGRLICGFLCPFGLIQDLLNKIPFPKKIRTFKGDKLLRKLKYVIFAVFVILLPLF
                     LVDIMGQGAPYFCKLICPAGTLEGGLPLVLLNKSMRSALGWLYIWKNVILVITIILSI
                     LIYRPFCKYICPLGALYSVFNPVSLYKYRVDKDKCIKCGKCAKACQMIVDPVENSNSP
                     ECIRCGRCRKVCPTDAIQCGIRRK"
     gene            complement(2166..2330)
                     /locus_tag="ACZPD3_06380"
     CDS             complement(2166..2330)
                     /locus_tag="ACZPD3_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HIR1415659.1"
                     /translation="MNKLKNSKIWNSKWPAVILMLVGVCFMGYGISRGELAVVFTKAI
                     NICMECVGIG"
     gene            complement(2331..3533)
                     /locus_tag="ACZPD3_06385"
     CDS             complement(2331..3533)
                     /locus_tag="ACZPD3_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR1415660.1"
                     /translation="MKNRRMRLMAIFAAGVLSVTSMGGIVTAHAEETLSVPAEEAGAG
                     AVQMDGKDAQAAEDGSVAASEGLIAGSFKPSEIAQPAQDTYEYPFLGMKLGISKDLKE
                     QMEKKNISMFTDERWNDNADAVTYATASWCTLTDEQKDAEVDKMGTGYDDWLKSLSRV
                     GVIGMYDEESQKDLDTITGCTEHKELGTSSDGKYKYYLSTNKDADADLLKDVEGIEVT
                     LTEMTPFQMLSAFDQPQDTSDSTEATEGTNVGKFETTGVDGKTYTQDIFSKYDLTMVN
                     IFTTWCSPCVNEIPDLEKLYQEMKDKGVGVVGVTLDTVGSGGKQDEEAVKKAQVLQKK
                     TKASYPFLIPDSGMMNGRLNGISAFPETFFVDKNGNIVGETYSGSHSLDEWKEIVEKE
                     LENVTEGK"
BASE COUNT          971 a          911 c          620 g         1166 t
ORIGIN      
        1 atattgcgca caaatgtggt ttcacgacca attcaacgtt caaccggagt cactcccatt
       61 atctgtctga agttccggtt aaacgttgaa ttggtcgtga aaccacattt gtgcgcaata
      121 tctgccaccg gtatatctgt tttctgcaga aattcacagg cagtatgaat ccgcacagaa
      181 ttaatatact ccaaaggact tactttcata tgcgatgtaa atactcttct gaaatgagtt
      241 tcactgatgt gacagatttt cgccaggtcc tcaatccgaa tgtcctccat atagtgataa
      301 gaaacaaaat ccagtacacg ggtgatcata tttgtaagtt tccctgtttc ctcttccacc
      361 ctgtcttccg gtgaaacccg gttcagtctg gcaatttcta ctagtaatgc cgcaagaact
      421 ccactggatt cttcaatata aaattcttct ccctcacgca tgatattcat gatcttaaga
      481 atttttgcag ctatagaggg ggactcattc tctttcagaa ataaagcctt agaatatatt
      541 ctctgaatca ttttttcagc ctttaccgga ttgttcagaa attttttcat gaaaccttcc
      601 acatctatga aaagatactc ccatttactg accgtaccca gatcactgtt agtcgtatga
      661 gcataattgg gtggaatcac cgtaaattct cttcccgaaa accgcatttt agcctcacca
      721 aacgccataa ctccatcccc ttcgtaacag aaaccaatct ccagataatt atgaaaatga
      781 agataatcca catcatttcc ataattctga acccacttct gtcccagaag agccaggatc
      841 ggacttccgg caggcatctg gtaatagcgg aattccatct tcggtttttt tctcttcttt
      901 gccatacagt cacctcatct tctgttctgt attttcataa tatgatttta ccagtttttt
      961 atatattttt cgatattttt gtacattttt caataaaaac atacaaaaat acctggcaga
     1021 ctacttttta tagtctgtca ggtatccctt ttccatatct gttattatca atctttttat
     1081 tcacaggctc atggctgaac ccgaataatt ttgactgata gcagttctat acatacctct
     1141 agtatcactt tattttcttc tgattccaca ctggattgca tctgtcggac agactttgcg
     1201 gcatcttcca cagcggatac attccggact gttggagttt tccacagggt ctacgatcat
     1261 ctggcatgct tttgcacatt ttccgcattt tatacactta tccttatcca ctctgtattt
     1321 atacagggac acaggattaa atacggaata aagtgctccc aagggacaga tatatttaca
     1381 aaacggacgg tagattaaaa tggaaagaat gattgttatg acaaggatca cattcttcca
     1441 tatgtacagc cagcccagtg cactacgcat ggatttattc agaagtacca gtggcagtcc
     1501 gccctccagt gttcctgccg ggcagataag cttacagaag tacggtgcac cctgtcccat
     1561 aatatccacc agaaacaatg gaagcaggat cacaaatact gcaaaaatca catatttcag
     1621 cttacgaagc aacttgtcgc ctttgaatgt gcggattttc ttaggaaatg gaatcttatt
     1681 cagcagatcc tgaatcaatc cgaagggaca gagaaaaccg cagatcagtc ttcccaccat
     1741 agctccaatg aagataagaa atcccaccac ataataagcc atcttgaaat tccagctccc
     1801 tatcacagcc tgaagagaac cgataggaca ggcgccgaca gcaccggggc aagaataaca
     1861 gttcaggccc ggaacacagg catttttcaa tttgcctgtg taaattttgc ctgtgacaaa
     1921 gccggtaaca tgactgtttg taatgaacgc ccacagtgcc tggattccgt ggcggggcca
     1981 ctcattgatc ttcatatttt ttgtgttttt tttcttatta cttttgttat cgctgttctt
     2041 tttattactc atgataattt attttctttc tattcatatc ttatccacag acttatctta
     2101 tgacaatgtt tcagtcagac ggatatttgc aacctccgca ggggaactta cctgattctg
     2161 ttaaatcatc caattcccac acattccata cagatattaa tggcttttgt aaacaccact
     2221 gccaactctc cgcgggaaat cccatatccc ataaagcaga ctcccacaag cataagaatc
     2281 acagccggcc atttactgtt ccatattttg ctgtttttta atttattcat ttattttccc
     2341 tctgtgacat tctccagctc tttttccacg atctctttcc actcgtccag actgtggctt
     2401 ccggagtagg tctcacctac aatatttccg tttttgtcaa caaagaaggt ttccgggaaa
     2461 gcagagattc cattgagacg tccgttcatc attccggaat ccgggatcag gaatggataa
     2521 gatgcctttg ttttcttctg aagtacctga gcctttttca ctgcttcctc atcctgcttt
     2581 ccaccactgc ctactgtatc cagtgtcacg cctactactc ccacaccttt atccttcatc
     2641 tcctgataaa gtttctccag atcaggaatc tcattgacac atggagaaca ccaggtggtg
     2701 aaaatattta ccatagtcag atcatatttg ctgaagatat cctgtgtgta agtttttcca
     2761 tcaactcctg ttgtttcaaa tttacctaca tttgttcctt cagtcgcctc tgtactgtca
     2821 gaagtatcct gtggctggtc aaatgcagag agcatctgga aaggagtcat ttctgtaagt
     2881 gttacctcaa tcccctccac atcctttaac aggtctgcat ctgcatcctt gtttgtgctg
     2941 agataatatt tatatttacc gtcactgctg gtacccagtt ctttatgctc tgtacagccg
     3001 gtaatcgtat ccagatcttt ctgggattcc tcatcataca ttccgatcac acctactctg
     3061 ctgagacttt tcagccagtc gtcatatccg gtacccatct tgtccacctc tgcatccttc
     3121 tgctcatctg tcagggtgca ccagcttgct gtggcatatg tgactgcatc tgcattgtca
     3181 ttccatctct catctgtaaa catggaaatg ttcttcttct ccatctgctc tttcagatct
     3241 ttggaaatcc ctaatttcat tccaaggaat ggatattcat aagtatcctg tgccggctga
     3301 gcaatctctg acggtttgaa agatcctgcg atcaagccct cggaagctgc cacactgcca
     3361 tcttctgctg cctgagcatc ctttccatcc atctgaaccg cacctgcacc ggcttcctct
     3421 gcaggcactg aaagagtttc ctctgcatgt gcagttacaa tacctcccat agatgtcaca
     3481 cttaatacac ctgctgcaaa aatcgccatt aatctcattc ttctgttttt cattataagt
     3541 aagctccctt cattttttac tgttatgtag gcgtttgcga aacgccacaa gccgcataaa
     3601 tacggcattt tttgttacct ataaacaaat aactcctcac cggatatggt ataatagagt
     3661 tgactaaa
//
LOCUS       DBMDXG010000200         3663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5823_length_3663_cov_1.640090, whole genome shotgun sequence.
ACCESSION   DBMDXG010000200 DBMDXG010000000
VERSION     DBMDXG010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5823_length_3663_cov_1.640090"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(203..457)
                     /locus_tag="ACZPD3_06390"
     CDS             complement(203..457)
                     /locus_tag="ACZPD3_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1415661.1"
                     /translation="MIKKPITINLSTGLEARPVAQLVQVASQFNSEIYVEIGKKRVNA
                     KSIMGMMTLGLDAGEEITLSANGEDEEAAMAGIEQYLSNQ"
     gene            complement(454..1413)
                     /gene="whiA"
                     /locus_tag="ACZPD3_06395"
     CDS             complement(454..1413)
                     /gene="whiA"
                     /locus_tag="ACZPD3_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR1415662.1"
                     /translation="MSFSGMVKEELSRQIGLARHCKMAELAAILCSCGKMECFSGDSK
                     LKIQTENEAVARKCFTLLQKTFNIETKIFVRENSHLKRVKVYTIEITDPEEIQVIFQA
                     LRLVTNSIDQGTLVLSDMLVVQQNCCKRAFIRGAFLASGSISDPEKGYHFEIVCSDAK
                     RAEQLQTIIRSFSVDAKIVQRKKSHVVYVKEGAQIVDMLAVMEANVALMDLENIRILK
                     EMRNSVNRKVNCETANINKTVNAAVKQMEDIKLVRQKIGFEQLNEGLAQVAELRMQYP
                     EATLKELGMMLSPQVGKSGVNHRLRKLSAMADELREKQGGELL"
     gene            complement(1424..2311)
                     /gene="rapZ"
                     /locus_tag="ACZPD3_06400"
     CDS             complement(1424..2311)
                     /gene="rapZ"
                     /locus_tag="ACZPD3_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR1415663.1"
                     /translation="MRFVIVTGVSGAGKTSALKMLEDAKYFCVDNLPIPLLEKFASLM
                     PEIHGEDVQNVALGIDARSGRSLDELEIVLDRMKKAGYDFEILFLDAQDSVLVKRYKE
                     TRRSHPLAMGGRVDDGIRMEREKMRFLKERADYIIDTSNLLTRELKQEIDRIFVDNQD
                     FCNMMISVLSFGFKYGIPADADLVFDVRFLPNPYYVDELRPLTGLDDRVFNYVMDCDI
                     ARTFADKLEDMINFLIPNYVKEGKTNLVIAIGCTGGKHRSVTLARELYSRLSGNTKYG
                     FRLEHRDAQKDRLVRKQEG"
     gene            complement(2404..3324)
                     /gene="murB"
                     /locus_tag="ACZPD3_06405"
     CDS             complement(2404..3324)
                     /gene="murB"
                     /locus_tag="ACZPD3_06405"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR1415664.1"
                     /translation="MKSVNQNIIEKFNDLLGEEKVRVDEPMKRHTTFRIGGPADYFLL
                     PSSEEELSGILKICKNEELPYFILGNGSNLLVSDEGYRGVIIQLYRNYGDITVKGNEI
                     HATAGALLSQIAVAAKNASLTGFEFAGGIPGTLGGAVVMNAGAYGGEMKDVLKEVTVM
                     TAAGEILVLPAEKLEMGYRTSLVKTKGYLVLSAVIVLEQGNQEAIKARMKELTEQRVS
                     KQPLEFPSAGSTFKRPEGYFAGKLIMDAGLRGYQTGGAQVSEKHCGFVINKDNATAAD
                     VCRLLRDVQDKVKEQFGVTLEPEVKFLGKF"
BASE COUNT          911 a          968 c          594 g         1190 t
ORIGIN      
        1 tcccgcgagg tgttttggca tgaatatgcc aaaatcccga gacatacaag ccaaaattcc
       61 attgccgtag gcaatctgga atgaattttg gctacgttac tgtgaacaaa gtgaacagta
      121 acattgctgt cgtatacacg acaaaacaca tccgaaaagc ggtcttccct tccggatgtg
      181 ttctatcctg aatactattt gatcactgat tacttaagta ctgctcaata cctgccatag
      241 cagcttcctc atcctcacca tttgcagata aagtaatttc ttcccctgca tcaagcccaa
      301 gcgtcatcat acccataatg ctctttgcat tcacgcgctt ctttccaatc tctacataaa
      361 tctcactgtt gaactgacta gccacctgta ccaactgtgc caccggacgc gcttccagac
      421 ctgtggataa attgatggtg attggttttt tgatcataat aattctcctc cttgtttctc
      481 ccgcagttcg tcagccattg cactcagctt tctcagtctg tgattgactc ccgatttgcc
      541 cacctgcggg cttaacatca ttcctaattc ttttagtgta gcttccggat actgcatccg
      601 aagttcagca acctgtgcaa gtccttcatt aagctgctca aatcctattt tctgccggac
      661 cagcttaata tcctccatct gcttgacggc tgcatttacg gttttattaa tattcgcagt
      721 ttcacagttt acttttctgt ttactgaatt tctcatttcc ttcagtatgc ggatgttctc
      781 caggtccatc aatgccacat tagcttccat aacagcaagc atgtcaacaa tctgcgcacc
      841 ttctttcaca tagaccacat gtgatttctt acgctgtacg attttggcat ctaccgaaaa
      901 actgcggatg atcgtctgta gctgctctgc tctttttgca tcagaacata caatctcaaa
      961 atgatacccc ttctcaggat cactgattga accggatgct aaaaaagctc ccctgataaa
     1021 cgcccgtttg cagcaattct gctgtaccac cagcatatcc gacaatacaa gcgtaccctg
     1081 gtcgatactg ttcgtgacaa gccgcagtgc ctgaaaaatt acctggattt cttcgggatc
     1141 agttatttct attgtatata ccttcacccg tttcaagtga ctgttctcac gtacaaatat
     1201 ctttgtttct atattaaatg ttttttgcaa taatgtaaag cactttcttg caactgcttc
     1261 attttccgtc tggattttca gtttactgtc ccctgaaaaa cattccattt tcccacagga
     1321 acataaaatc gcagccagtt ctgccatttt gcaatgtctg gccaatccaa tctgtctgga
     1381 aagttcttcc ttcaccatcc ctgaaaaaga catctctcct ttatcatcct tcctgttttc
     1441 tgaccagtcg gtctttctgt gcatctctgt gttccagcct gaatccgtat tttgtatttc
     1501 cagacaatct ggaataaagt tctcttgcca gcgtgacgga acgatgcttg ccgcctgtac
     1561 atccaattgc gatcaccaga tttgtctttc cctctttcac ataattcgga ataaggaaat
     1621 tgatcatatc ttccagttta tccgcaaatg tcctggctat atcacaatcc attacataat
     1681 taaaaaccct gtcatccagc cctgtcagcg gtctgagttc gtccacatag tatggattcg
     1741 gcaggaaacg cacgtcaaac acgagatccg catctgccgg tattccatat ttaaatccaa
     1801 atgacagtac agaaatcatc atattgcaga aatcctgatt atctacaaat atcctgtcga
     1861 tctcctgttt cagttctctt gtcagaagat tgctggtatc aatgatataa tccgcacgct
     1921 ctttcagaaa acgcatcttc tctctttcca tccggattcc atcatccact cttcccccca
     1981 ttgcaagagg atgacttctg cgggtttctt tataacgttt gaccagaaca ctgtcctgcg
     2041 catccagaaa caggatttca aaatcatacc ctgccttttt cattctgtcc agtacaatct
     2101 ccagctcatc cagagaacgc ccgcttcttg catcgatccc cagtgcaaca ttctgcacat
     2161 cttctccatg tatctcaggc atcagtgatg caaatttctc cagtaaaggg atcggcagat
     2221 tgtctacaca aaaatacttc gcatcctcca gcattttcaa tgcagatgtt tttcctgctc
     2281 ccgatactcc tgtaactatt acaaaacgca ttttctatct cccgtcactt cttaatttca
     2341 tctgttgctc attggtcttt ggtctttgca ctctgttcac agtacttttt cgattcagtc
     2401 agatcaaaat ttcccaagaa atttcacttc cggttcaaga gttacgccaa actgttcttt
     2461 tactttatcc tgtacatctc tgagcagtct gcacacatct gctgcagttg cattatcttt
     2521 attgatcaca aacccacagt gcttctcgga aacctgtgca ccgcctgtct ggtatcctct
     2581 cagccctgca tccatgatca gctttccggc aaaataccct tcaggacgtt tgaatgtgct
     2641 tcccgcactt ggaaattcaa gaggctgttt gcttactctc tgttctgtca gttctttcat
     2701 tcttgctttt atggcttcct gattcccctg ttcaagaacg atcactgcgc tcaacaccag
     2761 atatcctttt gttttcacaa gacttgtgcg atatcccatt tccagcttct ctgccggaag
     2821 tacgagaatt tctcctgcag ctgtcataac agttacttct ttcagcacat ctttcatttc
     2881 accgccatag gcacctgcat tcatgacaac tgcaccgcct aatgtgccgg gaattcctcc
     2941 ggcgaactca aatcctgtca gcgacgcatt tttggctgcc accgcgatct gtgacagcag
     3001 agctcctgct gttgcatgga tttcatttcc ttttactgta atatcaccat aatttctgta
     3061 aagctgaata atgacacccc tgtatccttc atcgcttacc agaagattac ttccatttcc
     3121 aagaataaaa tatggcaatt cttcattttt gcatatcttc agaatcccac tcagttcttc
     3181 ctcagaagac ggaagcaaaa agtaatctgc cggaccgcca atccgaaatg tggtatgacg
     3241 tttcatgggt tcatccactc tcactttctc ttctcccagc agatcattaa atttctctat
     3301 tatattctga ttcacagatt tcattcctca atctttctat atttattatg atttattatg
     3361 tttcttttga cgttttcatt ctgtcgaatt ttccgatcag ttcctgcagc gtcagataat
     3421 acagtcatta tactgttact gttcactccg ttctcagtaa cttagccaaa attcattcca
     3481 gattgcctgc ggcaatagaa ttttggcttg tatgtctcag gattttggca cattcatgca
     3541 aaaatacctc gcgggatagc ggtgtgtgaa cagtaacatt atactatctc tctctccaaa
     3601 agtacagtta ttcatacact cttttggaga gagagatagt ataatgttac tgttcacaca
     3661 ccg
//
LOCUS       DBMDXG010000201         3651 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5847_length_3651_cov_1.486197, whole genome shotgun sequence.
ACCESSION   DBMDXG010000201 DBMDXG010000000
VERSION     DBMDXG010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5847_length_3651_cov_1.486197"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="ACZPD3_06410"
     CDS             complement(<1..1396)
                     /locus_tag="ACZPD3_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR1415665.1"
                     /translation="MGVQWTKEQQEVIRLRDRNILVSAAAGSGKTAVLVERILSKITD
                     KEHPVDIDRLLIMTFTRAAAGEMKERISAAIEKALCEDPDNEHLQRQTTLLHTAQITT
                     IDGFCAYIIRNYFHLIGLDPGYRTADEGELKLLRGDVVKALLEEYYARKDEKFQKFVE
                     CFATGKSDENLGNLIQKLYEMAMSNPFPQEWLSGCMDDYRIDSLEELRETEWMRMLWD
                     AVKDELQEAKLLVQEARRICSEPDGPYLYDEALSSDLLLIRSLQELAEKRDYNGIAEI
                     LMKPSFARLSTKKAADVDEQKKQRVKDLRDEEKGILKELGQRYFQSAEKELLEVIRYI
                     RGPIEMLVELTARFKKQFGEAKREKNILDFTDMEHFALQILMTKEGEEIHMSQAAREL
                     SAKYDEVLVDEYQDSNLVQELLTTAVSGWINQKKNIFMVGDVKQSIYRFRLARPELFM
                     EKYKSYSTEEAQEQR"
     gene            complement(1398..>3651)
                     /locus_tag="ACZPD3_06415"
     CDS             complement(1398..>3651)
                     /locus_tag="ACZPD3_06415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR1415666.1"
                     /translation="NPFVEYLRAAMEMAVQGFPYESVFRYLRCGMSEVTREQADKLEN
                     YVLALGIRGYKKWSEKWVRVYRGMEAEKIQELNEIREIFAEEVRELAQGFGSGKKTVE
                     EYCRILYEFIQKSNVWQKLKRQERKFKESGDKAMEKEYNQIYGIVMDLLDKMVEILGE
                     ETVNRQEFRQLLESGLSQAKVALIPPSIDQVMVGDMERSRLKEIKALFFVGVNEGNIP
                     KSTQTGGILSELDRDFFQEQGVELAPGPKELMNMQRFYLYLNMTKPGEKLILSYSDTN
                     AKGEGISPAYLIGSIRSLYPKLGIEGGAGVRPHKNSINNYCYPENPEAGIDLFLEKLV
                     QETEKEHEDILEQADETDAMFGELYSWYLRNPEYRSRVQKLVQSAFAGKPEDIISQSV
                     AKALYGEVSPYSATRLERFAACAFAHFLQYGMKLTERVEYEFKPMDMGNVMHEALESF
                     AEEVRKRGMKWTELTEQERNEIADRCLDNIVADYGNTVLKSSARNEYMIERTRRILRR
                     TVWALQKQLEQGEFQPEGFEVTFGGGRIDRVDIMEDQNKVYVKVIDYKTGNTSFDLVY
                     LYHGLQLQLMIYLDGALRVEQKKYPDKEIIPAGVFYYNIKDPMIQEKIDADVEAVSAG
                     LMKELKMNGLAQADPELVYRMDSSLGSIPVAFNKDGSFRKNSSVADRTQFAVLGRYVR
                     TKIEKIRSSILEGDAEVSPYELGKKNACTYCPYMTVCGFDRRLSGYEFRRLKNFSDEE
                     LWKAFDREAE"
BASE COUNT          845 a         1061 c          595 g         1150 t
ORIGIN      
        1 ttctctgctc ctgtgcttct tctgtggaat aacttttata tttttccatg aacagctccg
       61 gccttgccag acggaaacga tagatactct gctttacatc tcctaccatg aagatatttt
      121 tcttctggtt gatccacccg gatactgctg ttgtcagaag ctcctgcact aagttactgt
      181 cctgatattc atccaccagg acttcatcat actttgcgga gagctctctc gcagcctgag
      241 acatatggat ttcttcccct tctttcgtca tcagtatctg cagggcaaaa tgttccatat
      301 ccgtaaaatc cagaatattc ttttctctct tcgcctctcc gaactgtttt ttaaatcgtg
      361 ccgtcaactc aactaacatc tctattgggc ctctgatata tctgatcact tccagaagtt
      421 ctttctctgc actctggaaa tatcgctgcc ccagttcctt cagaatcccc ttttcctcat
      481 cccggagatc tttcactctc tgcttcttct gttcatctac atccgcagcc ttctttgtgc
      541 tcagccgggc aaatgagggt ttcataagta tttctgcaat tccattataa tctcttttct
      601 ctgccagttc ctgcagactt cttataagaa gcagatcact gctcagtgcc tcatcataca
      661 gatacggacc atccggctct gagcagattc ttcgagcctc ctgcaccaga agcttcgctt
      721 cctgcagttc atccttcact gcatcccaga gcatcctcat ccactcagtc tctcttaact
      781 cttccaggga atcaatcctg taatcatcca tacacccgga cagccattcc tgcggaaaag
      841 gattgctcat agccatttca tataatttct gtatcaggtt tcccagattt tcatctgact
      901 ttcctgttgc aaagcattcc acgaatttct gaaacttttc atctttcctg gcataatatt
      961 cttcaagcag tgctttcaca acatctcctc gcagaagctt cagttccccc tcatctgccg
     1021 tccgatatcc cggatccagt ccgatcagat gaaagtaatt ccggataata taggcacaga
     1081 aaccatcaat agttgtaatc tgtgcagtat gaagcagagt tgtctgtctc tgcagatgct
     1141 cattatcggg atcctcacag agtgcttttt caatagctgc cgagatcctc tctttcattt
     1201 cacctgctgc agctcttgta aatgtcatga tcagcagtct gtcaatatcc acagggtgtt
     1261 ctttatctgt gatcttactc aggatacgtt ctaccagtac tgccgttttt ccggacccgg
     1321 ctgccgcact gaccagaata tttctgtccc gcagccgtat aacctcctgc tgctcctttg
     1381 tccactgtac acccatctta ttctgcctcc ctatcaaatg ctttccataa ttcctcatca
     1441 gagaagtttt tcagtctgcg gaattcatat cctgacaacc tgcggtcaaa tccgcatacc
     1501 gtcatatacg gacaataagt acacgcattc ttttttccga gctcataggg agaaacctct
     1561 gcatctccct ccagaataga acttcttatc ttctcaatct tcgtccttac ataccgtcca
     1621 agcactgcga actgtgttct gtctgccaca gaagaatttt tgcgaaagct tccgtcctta
     1681 ttaaatgcga caggaatgga gccaagactg ctgtccatcc tgtacacaag ctccggatct
     1741 gcctgagcca gcccattcat cttcagttct ttcataagac ctgcactgac tgcttccaca
     1801 tccgcatcta tcttctcctg gatcatagga tccttgatat tgtaataaaa aactcccgca
     1861 ggaataatct ccttatccgg atatttcttc tgctctacac gcagagcacc atccagatag
     1921 atcatcagct gtaactgcag gccatgatac agataaacca ggtcaaagga tgtatttccg
     1981 gttttataat caatcacctt cacatacacc ttattctgat cttccataat atctactctg
     2041 tcaatccgtc ctcccccgaa ggttacctca aagccttctg gctgaaattc tccctgttcc
     2101 agctgtttct gaagtgccca taccgtcctt ctgaggattc tccttgttct ctcgatcata
     2161 tattcatttc tggcactgct tttgagcacc gtgtttccat aatctgccac aatattatcc
     2221 agacaccggt ccgcaatctc attccgttcc tgttctgtca gctctgtcca tttcatgcct
     2281 ctttttcgca cttcttctgc aaagctttcc agtgcttcat gcataacatt tcccatatcc
     2341 ataggtttaa actcatattc cacacgctcg gtaagcttca ttccatactg aaggaaatgt
     2401 gcaaatgcac aggctgcaaa acgttccagt ctggtagcac tgtaaggtga aacctctccg
     2461 tataatgcct tcgccacact ctggctgata atgtcctctg gttttccggc aaaagccgac
     2521 tgtaccagct tctgtactct gcttctgtat tccggattcc gcagatacca gctataaagc
     2581 tctccaaaca ttgcatccgt ctcatctgcc tgctccagaa tatcctcatg ttctttctct
     2641 gtctcctgta ccagcttctc cagaaacaga tcaatcccag cttccggatt ttccggataa
     2701 caataattgt ttatagaatt tttatggggt ctgaccccgg ctccaccctc aattcccagc
     2761 ttcggataca gactgcgtat actcccaatc agatatgccg gacttatgcc ttctcctttt
     2821 gcattggtat cactatagga gaggatcagc ttttctcccg gtttcgtcat attcagatac
     2881 agataaaacc tctgcatatt catcagttct ttaggccccg gtgccagttc cactccctgc
     2941 tcctggaaga aatccctgtc aagctctgat aagattccgc ctgtctgtgt gctctttggg
     3001 atatttccct cattcacgcc aacaaaaaac aacgccttaa tctcctttaa gcgggatcgt
     3061 tccatatcac ccaccattac ctgatctatg ctcggcggaa tcagcgctac ttttgcctga
     3121 gaaagtcctg attccagaag ctgtcggaac tcctgtctgt ttaccgtttc ttcgccaaga
     3181 atctcaacca tcttatccag caggtccatc acaattccat aaatctggtt atattctttt
     3241 tccattgctt tatcgcctga ctctttaaac tttcgctcct gtcgtttcag tttctgccag
     3301 acattgcttt tttggataaa ctcatagaga atccggcagt actcttccac tgtcttcttc
     3361 ccggaaccaa atccctgtgc aagttccctt acttcttccg caaagatttc tctgatctca
     3421 ttcagttcct gtatcttctc cgcttccatt cctctgtata cacgaaccca tttttcagac
     3481 catttcttat atccccggat tcccagcgcc agcacgtaat tctccagctt atcagcctgt
     3541 tctctggtaa cttctgacat cccgcatctg agataacgga aaacactctc ataaggaaat
     3601 ccctgtaccg ccatctccat ggcagcgcgc agatactcca caaaaggatt c
//
LOCUS       DBMDXG010000202         3648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5850_length_3648_cov_2.204962, whole genome shotgun sequence.
ACCESSION   DBMDXG010000202 DBMDXG010000000
VERSION     DBMDXG010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5850_length_3648_cov_2.204962"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="ACZPD3_06420"
     CDS             <1..887
                     /locus_tag="ACZPD3_06420"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR1415667.1"
                     /translation="YVLDFSRKEVVDCIYEMMYKILSEAKVSYIKWDMNRSITECYSA
                     ALPADRQGEVFHRYILGVYDLYERLNTAFPQILFESCASGGGRFDPGILYYAPQGWTS
                     DDTDAAERVKIQYGTSMCYPVSSMGSHVSVVPNHQLNRKTPLHTKANVAYFGTFGYEL
                     DLNKLSDEEISEVKQQITFMKEYRELIQFGTFYRLKSPFEGNETAWMTVSEDKKTALV
                     FWYRERNVVNADFTRVRLQGLDPDLIYRNEYNETENYGDELMNLGLLTTDCTAGEPTS
                     EDEPCTDYESRIYVLTAK"
     gene            complement(971..1876)
                     /locus_tag="ACZPD3_06425"
     CDS             complement(971..1876)
                     /locus_tag="ACZPD3_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1415668.1"
                     /translation="MPNVKYTDSATFRCLENLKEGSLDISLIHTGKEHCPPGHICSMP
                     RDEFIIHFVLDGTGFYSARGQTWSLTPGQMFVIYPNEPVTYGADETNPWTYAWIGFRG
                     IRAHSMVKECGFSKNQLVLPAPDQKIILKHIDYMLNHKQLSKANDLRRQAYLILLLAE
                     LAGFHEKQSAQNKKNAKYAYSTSVYVELAIEYIKDMYQKGIGISDIADNIGISRAYLN
                     SSFQKELGMSAQTFLIDYKMHKAASLLVSTSLSVKEIANNVGYEDQLVFSKAFKKKFG
                     MSPKNYKTHKEMMDKFNEKQVDDPV"
     gene            2120..3385
                     /locus_tag="ACZPD3_06430"
     CDS             2120..3385
                     /locus_tag="ACZPD3_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1415669.1"
                     /translation="MRKNMKKLTVATVIGAMTVTALAPVSAQAKDKTLTVAIWDNGQK
                     AGLQEIMDEFTKETGIKTELQVVEWSSYWTLLEAGASGGDMPDVFWMHSNEAVRYMSN
                     DILLDLTDKIADSDKLEMDKFPKDIKEMYQWDGKTYAVPKDIDTIAMWYNKDMFDEAG
                     IDYPDGSWTWDEFYDIAEKLTKEDGSQYGFAANPSNEQDTWMNIVYSMGGRVLTDDNK
                     SGFDDPNTIKAMEFVDKCVKNVMPPASTMSETGTDVLFGSGKVAMISQGSWMVSAFKD
                     NDYIKEHCDVARLPKDAETGESVSIYNGLGWAASANTDMPDEAFQLIEWFGTKDMQQK
                     QADLGVTMAAYEGMSDGWVNSVDCFNLQPYMDAMDNIVFRPHTNATLAWWNPMCEELK
                     KPWNDEESMDDACKNIVTIMNDAIAEESY"
BASE COUNT         1181 a          702 c          831 g          934 t
ORIGIN      
        1 agtatgtgct ggacttctcc agaaaagaag tggtggactg tatttatgag atgatgtaca
       61 aaatcctctc agaagcaaag gtttcctaca tcaaatggga tatgaacaga agtattacgg
      121 aatgttattc agctgcactt cctgcagacc gtcagggaga agtgttccac agatatattc
      181 tcggagttta tgatctgtat gagcggttaa atactgcatt tccgcagatt ctctttgagt
      241 cctgcgcaag cggtggcgga cgttttgatc cgggaatcct ttattatgcg ccacagggat
      301 ggaccagtga tgatacagat gcggcagagc gtgtgaaaat ccagtacggc acaagcatgt
      361 gttatccggt aagctccatg ggaagtcatg tttctgtagt accaaaccat cagttaaacc
      421 gcaaaactcc gttacataca aaagcaaatg tagcttattt tggaaccttc ggatatgagc
      481 tggatctgaa taaactgtct gatgaggaaa tcagtgaagt aaaacagcag attaccttta
      541 tgaaagaata cagagaactg attcagtttg gaacttttta cagactgaag agtccttttg
      601 agggcaatga aacagcgtgg atgacagtca gtgaggacaa aaaaacagcg ctggtattct
      661 ggtacaggga aagaaatgta gtaaacgcag attttaccag agtgcggctt cagggactgg
      721 atccggatct tatctacaga aatgagtata atgaaacaga aaactacggg gatgagctga
      781 tgaatctggg actgctcacc acagactgta cagcaggtga acctacaagt gaagacgaac
      841 catgcacaga ctatgagtcc agaatttatg ttttaactgc aaaataataa ccgaatcagg
      901 taagctcctt atggaggttg cgaaaagcaa tagacagaaa taaaaaatca acaaaaagca
      961 ggcagaacga tcacacggga tcgtctacct gcttttcgtt aaatttatcc atcatttctt
     1021 tatgggtctt ataattcttc ggactcattc caaacttctt cttaaaagcc ttggaaaaaa
     1081 caagctggtc ttcatagccc acattattag caatctcttt aacagacaga ctggtactga
     1141 ccagaagact ggctgcttta tgcatcttat aatcaatgag gaaggtctgt gcggacattc
     1201 caagctcttt ctgaaaggaa ctgttcagat aggcacggct gattccgata ttatctgcaa
     1261 tatcagaaat cccgattccc ttttgataca tatctttaat atattcaatg gcaagctcca
     1321 cgtagacact ggtgctgtag gcatatttgg catttttttt gttctgtgca gactgctttt
     1381 catgaaagcc ggcaagctcg gctaacaaca gaatcagata agcctggcgc cttagatcat
     1441 tggctttgga aagctgctta tgattcagca tataatcgat gtgtttcagg attattttct
     1501 gatccggtgc aggaagtact aattgatttt tggaaaatcc acattctttt accattgagt
     1561 gagctcggat tccgcgaaaa ccaatccagg cataagtcca gggattggtt tcatcagcac
     1621 cgtatgtgac tggttcattt ggataaatta caaacatctg tccgggagta aggctccagg
     1681 tctgacctct ggcagaatag aatccggtac cgtccagcac aaaatggatg atgaactcat
     1741 ctcttggcat ggaacagata tgtccgggcg ggcagtgttc tttgcccgta tggatcagac
     1801 tgatatcaag ggagccctct ttcaggtttt caagacagcg gaaagtagcg ctgtccgtat
     1861 attttacatt tggcatttgg gtaattcctt tttctatttt ctttgattgc agatattgtt
     1921 gatataaaga cataaggact gatatcctgc aaatacgcat aaattcaagt cgttgttgac
     1981 attttaccat aaattcagcg ctttttccat aacgattatg acataaattc atttcattag
     2041 tccatttaaa aaaatagaca aaaacgtata ataagaatca caaggaacac gtcgaaacga
     2101 cgaataaagg aggatgtata tgagaaaaaa catgaaaaaa ctcacagtcg caacagtgat
     2161 cggtgctatg acagttactg cacttgcacc agtaagcgca caggcgaaag acaaaactct
     2221 gacagttgca atctgggata acggacagaa agccggactt caggagatca tggatgagtt
     2281 tacaaaagag accggaatca aaactgaact tcaggtagta gagtggagca gctactggac
     2341 acttcttgaa gcaggtgcat caggcggaga catgccggac gtattctgga tgcattccaa
     2401 tgaagctgta agatacatgt ccaatgatat cttacttgat ctgacagaca agattgcaga
     2461 cagcgacaaa ctggaaatgg acaaattccc gaaagatatc aaagaaatgt atcagtggga
     2521 cggaaaaaca tacgcagtac cgaaggacat cgatacaatt gctatgtggt acaacaaaga
     2581 tatgtttgat gaagcaggaa tcgattatcc ggacggaagc tggacatggg atgaattcta
     2641 tgacattgca gaaaaactta caaaagaaga cggaagccag tatggtttcg cagcaaaccc
     2701 gtctaacgag caggatacat ggatgaatat tgtttactcc atgggcggac gtgtactgac
     2761 agatgacaac aaatccggtt ttgatgatcc aaacactatc aaagcaatgg aattcgttga
     2821 caaatgcgtt aaaaatgtaa tgcctcctgc atctacaatg tccgaaacag gtacagacgt
     2881 actcttcgga tctggcaagg tagctatgat ttctcaggga tcctggatgg tatctgcttt
     2941 caaagacaac gattacatca aagagcactg tgatgtggca agacttccga aagatgctga
     3001 aacaggagag agcgtatcta tttacaatgg tcttggatgg gcagcatctg caaacacaga
     3061 tatgccggac gaagctttcc agcttattga gtggttcggt acaaaagata tgcagcagaa
     3121 acaggctgac cttggtgtaa caatggcagc ttacgaagga atgtccgatg gatgggttaa
     3181 cagtgttgac tgcttcaact tacagccata catggatgca atggacaata tcgtattcag
     3241 accacacaca aatgcaacac ttgcatggtg gaacccaatg tgcgaagaac tgaagaaacc
     3301 atggaatgat gaagaaagca tggatgatgc atgcaagaat atcgttacta ttatgaacga
     3361 tgccatcgca gaagagtcct attaattaat attcagcaaa accattcata aaaaataaga
     3421 aacaggaaag ctgccgcaga aaagattccg atcatgtggt aaccgcagaa gtcacaacag
     3481 gtatgcagat gtgcggcagt tttccgttca aatcagcaac agaattttac gataagaagg
     3541 agtgagaacc gtggcaaatc caaaaaaagg taccagtcag gcgcggaatg aattcatctg
     3601 gggatgggcg ttcattcttc ctacaatgct tggtctgatc atcttaaa
//
LOCUS       DBMDXG010000203         3644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5861_length_3644_cov_1.991533, whole genome shotgun sequence.
ACCESSION   DBMDXG010000203 DBMDXG010000000
VERSION     DBMDXG010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5861_length_3644_cov_1.991533"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="ACZPD3_06435"
     CDS             complement(<1..683)
                     /locus_tag="ACZPD3_06435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1415670.1"
                     /translation="MKKWKYLLKVVMAVGVIAMTAVQICTAAEGTAQAAVSEVTPVSI
                     STNEISGWPAGPEITSETGVLMDADSGTLLYSKGGDEIRYPASITKIMTLLLAVENCS
                     LKEDVVFTETGTRDISWDSGNIGMQVGEVMSMRACLYALVIRSANEVAAQIAEHVGGT
                     EQHFVDMMNERAAQIGCTNTHFVNASGLPDPDHYSTAHDMALIMREGLKNKKFRRIIG
                     ATDYTIKPT"
     gene            complement(769..1899)
                     /gene="rffA"
                     /locus_tag="ACZPD3_06440"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(769..1899)
                     /gene="rffA"
                     /locus_tag="ACZPD3_06440"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HIR1415671.1"
                     /translation="MIDFNRPAFTGREFDYIRDAVQRGMLCGDGEYTKRCSRWMMDKF
                     HVNHVMLTTSCTHALEMAAHLCDIKPGDEVIMPSYTFVSTADAFVLKGAKIVFVDIRP
                     DTMNIDEKLIEAAVTEKTKVIVPVHYAGVACEMDTIMEIAKKYNLKVVEDAAQGVDAC
                     YKGKALGTIGDFGCYSFHETKNYTMGEGGAILFNRDEYLEKAEILREKGTDRSKFFRG
                     QVDKYRWIDYGSSYLPSELNAAYLYAQLEARNQILAKRMEIYNYYHKNLAHLAQEGKI
                     EQPYVPEECSHNAHMYYIKVRDIQVRTRLIAYLREKGICSVFHYVPLHSAPAGQKFGR
                     FSGEDVYTTKESERLLRLPMFYNLDMEDVKYITDTIASFDGF"
     gene            complement(1892..3190)
                     /locus_tag="ACZPD3_06445"
     CDS             complement(1892..3190)
                     /locus_tag="ACZPD3_06445"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR1415672.1"
                     /translation="MKHKVLILGTLGEFTELVKKAKEKGYETVVCDGYADGIARTYAD
                     KAYTIPVTDVDAIALMCREEGVDGIITSFSDLLLECMVKIADKAGLPCYLKPEQLSWY
                     RDKSACRDVLDKLGLPAPGFRKISVELLKQGSEEEIQQSIANLQYPLISKPLDKYGSR
                     GIFIIHHSDEVRKKALQTAEYTDCQEILVEEYNDGYEFNMMTWVMDGKVNVISIADRE
                     KTEMEEGMLPLSTRNVYPSRFLAEVEKSATDILQNYIRYTGQTEGALSMQFFWKPGRG
                     IQVCEIAGRFFGYEHELTDMVYGFQTEELLLDYLYEKDRIKEMFDRHDIYHPVKYGAV
                     LYFQGRQLQIADQTAACELAKEKCVVKPWIFYKEGEHVIEYGPNPYLALYYIETENRR
                     QLEMETEKFFSEMSIRDPEGREVAYRNKIPEYEKEKKTDD"
     gene            complement(3187..>3644)
                     /locus_tag="ACZPD3_06450"
     CDS             complement(3187..>3644)
                     /locus_tag="ACZPD3_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415673.1"
                     /translation="LGRYEHNAGTYYKDMLENAAKYDGMELRQMSKLTQNLLKAVDYD
                     RAKKKREENYRILGELLPSESIFNQTVPEGPFAYPYFHADGMKLRRYLAEKKIFVPTY
                     WKNIIENSETKSLEYTWAANILPLPCDQRYSVEDMKYMASVVRECEERI"
BASE COUNT          926 a          949 c          641 g         1128 t
ORIGIN      
        1 ttcgtaggtt tgatcgtata atctgtagcg ccgatgatcc tgcggaattt tttattcttc
       61 agaccttccc gcatgatcaa cgccatgtca tgtgcagtag aatagtggtc aggatccgga
      121 agaccactgg catttacaaa atgtgtattt gtgcatccga tctgtgccgc acgttcattc
      181 atcatatcta caaagtgctg ctctgtgccg cctacatgtt ctgcaatctg ggctgcgacc
      241 tcgtttgcag agcggatcac aagtgcataa aggcatgctc tcatgctcat cacttctccc
      301 acctgcattc caatattgcc ggaatcccaa ctgatatcac gtgttcctgt ttccgtaaag
      361 acaacatctt ctttcaggga acagttctcc accgccagaa gcagtgtcat gattttggta
      421 atacttgccg gataacggat ttcatctccg cccttgctgt acagaagagt tccgctgtct
      481 gcatccatca gtacccctgt ctcagatgtg atctcaggac ctgcaggcca gcctgaaatc
      541 tcattagttg aaatggatac gggtgtaacc tctgaaacag cagcctgcgc agttccttcc
      601 gcagcagtgc agatctgaac agcagtcatt gcaatgactc ccacagccat aacaaccttt
      661 aataaatatt tccatttttt cataatattt cctcttaata tatgtctctg ttttccgtcc
      721 gatgtaccga caggttgatt cctgaaacaa acttgcggca cacgattatc agaatccgtc
      781 aaaagaagct atggtatctg tgatatactt cacatcctcc atatccagat tataaaacat
      841 cggcagtctc aacagacgtt cactttcctt tgtagtataa acatcttccc ctgaaaatct
      901 tccaaatttc tgtcccgcag gtgcagaatg aagaggcacg taatgaaata cgctgcagat
      961 tcctttttcc cgaaggtaag caatcagccg ggttcttacc tgtatatcac gcaccttgat
     1021 ataatacata tgggcattgt ggctgcattc ctccggcaca taaggctgtt cgatcttacc
     1081 ttcctgtgca agatgagcca gatttttatg ataataattg tatatctcca ttcttttggc
     1141 aagaatctga tttcttgctt ccagctgtgc ataaagatat gctgcattca gttcacttgg
     1201 aagataggaa gaaccataat ctatccagcg gtacttgtca acctgtccgc ggaaaaactt
     1261 acttctgtca gttccctttt ctctcaggat ctctgccttc tccagatatt catccctgtt
     1321 aaaaaggatt gcaccgccct ctcccatagt atagttcttt gtctcatgga aactgtaaca
     1381 cccaaaatct ccaatggtac caagagcttt tcccttataa caggcatcta ctccctgcgc
     1441 agcgtcttca acaaccttca gattgtattt ttttgcaatc tccataatgg tatccatctc
     1501 acaggcaacc cctgcatagt ggaccgggac gatcactttt gttttctcag tcactgccgc
     1561 ctcgatcagt ttctcatcaa tattcattgt atccggtctg atatcaacaa atacaatctt
     1621 tgctcccttt aacacaaagg catctgcggt tgaaacaaat gtataggacg gcatgatcac
     1681 ctcatcaccg ggcttaatgt cgcaaagatg ggctgccatc tccagagcat gggtacagga
     1741 tgtggtaagc attacatgat ttacatgaaa tttatccatc atccaccgag aacatctttt
     1801 cgtatattct ccatccccac agagcattcc tctctgtacc gcatcacgta tgtaatcaaa
     1861 ttccctgcct gtaaaagcag gtctgttaaa atcaatcatc tgttttcttt tccttctcgt
     1921 attccggtat cttatttctg taggcaactt ctcttccctc cggatcccgg atactcatct
     1981 cactgaaaaa tttctcagtc tccatctcca gctgcctgcg gttttctgtt tctatataat
     2041 acaatgcaag atatggattc ggcccatatt ctatcacatg ttcgccttct ttataaaaaa
     2101 tccagggttt tacaacgcat ttttcttttg caagctcaca ggctgccgtc tgatctgcaa
     2161 tctgaagctg tcgtccctga aaatacagta cagccccata ttttaccgga tgataaatat
     2221 catgacggtc aaacatctcc tttatccggt ctttttcata aagataatcc agcagcagtt
     2281 cctctgtctg aaatccataa accatatctg tcagctcatg ttcatatcca aagaaacgtc
     2341 ctgcaatctc acatacctga atgcccctgc caggtttcca gaaaaactgc atggataatg
     2401 caccttccgt ctgtccggta tagcggatat aattctgcag aatatctgtg gcactttttt
     2461 cgacctccgc caggaaacgg gatggataaa catttcgtgt gctgagcgga agcattccct
     2521 cttccatttc cgttttctca cgatctgcaa tactgatcac attgactttc ccatccatta
     2581 cccatgtcat catgttaaac tcataaccgt cattgtattc ctcaaccaga atttcctgac
     2641 agtccgtata ttccgctgtc tgcagggctt tttttctgac ttcatctgaa tgatgaataa
     2701 taaagattcc tcttgaacca tatttatcca gaggtttact gatcagagga tactgcagat
     2761 ttgcaatact ctgttgtatt tcttcttctg atccctgttt cagcagttcc acagatattt
     2821 tccgaaatcc cggtgccggc agtccaagct tgtccagaac atcccggcat gccgatttat
     2881 ccctgtacca ggaaagctgt tccggtttca gataacaggg cagtcctgct ttgtctgcaa
     2941 ttttaaccat acactccagg agcagatctg aaaatgatgt gatgatcccg tccacacctt
     3001 cttccctgca catcagggca atagcatcta catctgttac aggaatggta taagccttat
     3061 ctgcatacgt tctggcaata ccatctgcat atccgtcgca cacaacagtc tcataacctt
     3121 tctcttttgc tttctttacc agctctgtaa attctccgag cgttcccaga atcaacactt
     3181 tatgtttcat atccgttcct cacattctct gacaaccgaa gccatatatt tcatatcctc
     3241 cacgctgtaa cgctgatcgc agggcaacgg aagaatattg gcagcccagg tatattccag
     3301 actttttgtc tcagagttct caattatatt tttccagtaa gtcggcacaa atatcttctt
     3361 ttcggcaaga tatctgcgaa gtttcattcc atcagcgtgg aaatacggat atgcaaaagg
     3421 tccctccggc acagtctgat taaatatact ttccgatgga agcagttctc cgagaatccg
     3481 gtagttttct tccctttttt tctttgctct gtcataatcc acagctttca gaagattctg
     3541 tgtaagcttt gacatctgcc gaagctccat cccgtcatat tttgccgcat tttccagcat
     3601 atctttgtag taggttccgg cattatgctc ataacgcccc agaa
//
LOCUS       DBMDXG010000204         3639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5866_length_3639_cov_1.319955, whole genome shotgun sequence.
ACCESSION   DBMDXG010000204 DBMDXG010000000
VERSION     DBMDXG010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5866_length_3639_cov_1.319955"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="ACZPD3_06455"
     CDS             complement(<1..235)
                     /locus_tag="ACZPD3_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HIR1415674.1"
                     /translation="MTTTIIVAIVAVVVALLIAVPVTCKVAVNNKIQKDAEIVGTAED
                     KARSIIDEALKTAETKKREALLEVKEESLRTKNE"
     gene            complement(410..997)
                     /locus_tag="ACZPD3_06460"
     CDS             complement(410..997)
                     /locus_tag="ACZPD3_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR1415675.1"
                     /translation="MGNSSGDLREFLRNAEALKRQTVQKQQKNLTEEPEEYRDARRKA
                     MRLLEHMDRTEKGLREKLRQAGFTSQAVDHALTYVEAYGYIDDERYARTYIAYRMDIK
                     SRQKIIRELMGKGIDRKTAINAWEEEAALNMPDEKEILYRTIEKKYPPDTELDEKKMR
                     RLYGYLVRRGFGYSDIADTLENMNIRLVHTYSDES"
     gene            complement(1000..2064)
                     /gene="recA"
                     /locus_tag="ACZPD3_06465"
     CDS             complement(1000..2064)
                     /gene="recA"
                     /locus_tag="ACZPD3_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR1415676.1"
                     /translation="MINEEKQKALEAALGQIEKHYGKGSVMKLGESGANMQVETVPTG
                     SLSLDIALGVGGVPKGRIIEIYGPESSGKTTVALHMVAEVQKRGGIAGFIDAEHALDP
                     VYAKNIGVDIDNLYISQPDNGEQALEITETMVRSGAVDIVIVDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTAVISKSNCVVIFINQLREKVGVMFGNPETTTGGRALKF
                     YSSIRMDVRRVETLKQGGEMVGNHTRIKVVKNKVAPPFKQAEFDIMFGTGISKEGDIL
                     DLAAECGIVNKSGAWYAYNGDKIGQGRENAKIFLKEHTDICDEIEKQVRIHYHLLPDE
                     EGQAKEPVPATGDAPDASEE"
     gene            complement(2399..>3639)
                     /locus_tag="ACZPD3_06470"
     CDS             complement(2399..>3639)
                     /locus_tag="ACZPD3_06470"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR1415677.1"
                     /translation="CYVGIMHMEYEGIQFYFIDNEYYFSGPKPYDGGTWDLEKFAFFS
                     KAVLSVLPVIGFRPDIIHCHDWQTGLVPVYLHDSFQQNEFFWNIKTIMTIHNLKFQGV
                     WDVQTIKNITGLSDYYFTADKLEAYKDANYLKGGIVFADAVTTVSNTYAEEIKTPFYG
                     EKLDGLMCARANSLRGIVNGIDYNEFNPETDPYITKTYNATTFRKEKVKNKLQLQRDL
                     GLQEDPKTMMVGIVSRLTDQKGFDLIAYVMDELCQDAIQLVILGTGDERYENMFRHFD
                     WKYHGKVSAQIYYDEKMSHRIYASADAFLMPSLFEPCGLSQLMSLRYGTLPIVRETGG
                     LKDTVVPYNEYEGTGNGFSFRNYNAHEMLATVRNAERIYYDKKREWNKMVDRAMAADF
                     SWGNSARQYEEMYNWLIGDK"
BASE COUNT          904 a          898 c          621 g         1216 t
ORIGIN      
        1 gttcattttt tgtacggaga gattcttcct tcacttccaa taaagcttct ctttttttag
       61 tctcagctgt tttcagagct tcatctatga tgcttcttgc tttgtcttct gctgtcccaa
      121 caatctcggc atccttctgg attttattgt tcacagcgac tttgcaagta acaggaactg
      181 caataagtag cgcgactacc acagcgacaa ttgcaacaat aattgttgtt gtcacaggag
      241 cacctcctta tttagttttc attgtacaaa tacaacaata caagtttatc tttttttcat
      301 acttatgtca agtttttcag gataattgtt tgaatatttt tcctatattt aattactgtt
      361 cgttctgttc tcagtaatat attttgcaat atacatacaa tggagtatac tatgattcat
      421 ccgaatatgt atgtacaagt ctgatattca tattctcaag agtatctgca atgtctgaat
      481 atccaaatcc tcttctcaca agataaccat agagccgacg catttttttc tcatccagct
      541 cagtatccgg cggatatttc ttttctatgg ttcgatagag gatttctttt tcatcaggca
      601 tatttaaggc agcctcttcc tcccatgcat ttatcgctgt tttcctgtct attccctttc
      661 ccataagttc ccggatgatc ttctgccggc tctttatatc cattctgtat gcaatataag
      721 ttctggcata tcgttcatca tctatatatc cataggcttc aacatatgtc agagcatgat
      781 caaccgcctg actggtaaat cccgcctggc gcaatttctc tctcaatccc ttctccgtcc
      841 gatccatatg ttctaagaga cgcatggctt tacgccttgc gtctctgtat tcttcaggtt
      901 cttccgttaa atttttctgt tgtttctgta cagtctgtct ctttaaagcc tctgcattcc
      961 tgagaaattc tctcagatct ccggatgaat tccccatgat cactcctcag aagcatccgg
     1021 tgcatcacct gttgccggca ccggctcttt ggcctgtcct tcttcatccg gaagaagatg
     1081 ataatggatt ctgacttgct tctcaatctc atcacagata tctgtatgct ctttcaggaa
     1141 aatcttcgca ttttcacgtc cctgtccgat cttatctccg ttatatgcgt accatgcacc
     1201 gctcttattt acaattccgc attccgctgc cagatcaaga atatctccct ctttggaaat
     1261 gccggttccg aacataatat caaattctgc ctgtttaaac ggcggagcca ccttattctt
     1321 cacaactttg attctggtgt ggtttcccac catctcaccg ccctgtttca atgtctctac
     1381 tctgcggaca tccatgcgga tagatgaata aaatttcaga gcacgcccac ctgtcgttgt
     1441 ctcagggttt ccaaacataa ctccaacctt ctcacgcagc tgattgataa agataactac
     1501 acaattagat ttactgatca ctgctgtaag tttacggaga gcctgtgaca tcagtctggc
     1561 ctgaagacct acatggctgt cacccatatc tccatcaatc tccgccttcg gtacaagtgc
     1621 ggcaaccgag tcaacgatca caatatccac agctccggaa cgcaccatcg tctctgtgat
     1681 ctcaagtgcc tgttctccat tgtccggttg tgatatgtac aggttatcga tatctacacc
     1741 gatattcttt gcatagacag gatccagagc atgttctgca tcaataaatc cggcaatccc
     1801 gcctcgtttc tgcacttccg caaccatgtg aagtgcaacg gttgtcttac cgctggattc
     1861 cggtccgtaa atctcgatga tacgtccctt gggaacacca cctactccaa gtgcaatatc
     1921 aagacttaaa gatccggtag ggactgtctc cacctgcata tttgctccgg attccccgag
     1981 tttcatgact gatcctttcc cgtaatgttt ctcaatctgt ccaagcgccg cttccagtgc
     2041 tttctgcttt tcttcattta tcatttattt taatctcctt cagcccttta agaacttatg
     2101 ttcgttttct attcattata gtattatact tcctgtcaaa tgtcaagctt tttattctgc
     2161 ctgaataacc ttttcatgtt actgttcact ccgttctcag taacgttgcc aaaatttatt
     2221 tcagattgcc tgtggcaatg gaattttggc ttgtatgtct cgggattttg gcatattcat
     2281 gccaaaacac ctcgcgggat aatggtgtgt aaacagtaac aacataacag taaaagcagg
     2341 gaatgtgctt tttttacatt ccctgctctt tcgtagtttt taatatttct ctctgtttct
     2401 atttatcacc aatcagccag ttgtacattt cttcatactg ccgtgcggaa ttaccccatg
     2461 agaaatcagc tgccatagca cgatcaacca ttttattcca ttcacgtttc ttatcataat
     2521 agattctttc cgcattgcgc actgttgcaa gcatctcatg cgcattgtag tttctgaaag
     2581 aaaatccgtt tccagttccc tcatattcat tataaggtac gaccgtatcc ttcagtccac
     2641 ctgtttctct gacgatcgga agtgttccgt aacggagact catcagctga ctcaaaccac
     2701 atggctcaaa taaagacggc atcaggaacg catctgctga tgcatagata cggtgagaca
     2761 ttttctcatc ataatagatc tgagcagata cctttccatg atatttccag tcaaagtgac
     2821 ggaacatatt ctcataacgt tcatcaccgg ttccaagaat tacaagctgg atagcatcct
     2881 ggcaaagctc atccatcaca tatgcgatca gatcaaaccc tttctgatct gtcagtcgtg
     2941 atacaatacc taccatcatc gtcttcggat cttcctgcaa tccaagatct ctctgcagct
     3001 gaagtttgtt cttaactttc tcttttctga aagttgttgc attataagtc tttgtgatat
     3061 atggatctgt ttcaggattg aattcattgt aatcaatacc atttacaatt ccacgcaggc
     3121 tgtttgcacg cgcacacatc agaccgtcca gtttttctcc atagaacggt gtcttgattt
     3181 cctctgcata agtattgctt acagtagtga ctgcatctgc aaacacaata ccgcccttca
     3241 gatagttggc atctttatat gcttccagtt tgtctgctgt aaaataataa tcggacagtc
     3301 ctgtgatatt cttgattgtc tgtacatccc atacaccctg gaatttaagg ttatgtattg
     3361 tcatgatcgt cttgatattc cagaaaaatt cattctgctg gaagctgtcg tgcagatata
     3421 cgggaacaag tccggtctgc cagtcatgac agtgtataat atccggtctg aatccgataa
     3481 ccggaagaac tgacagtaca gctttggaga agaatgcaaa tttctccaga tcccatgttc
     3541 ctccatcata cggtttgggt ccgctgaaat agtattcatt gtcaataaaa taaaactgaa
     3601 ttccttcgta ctccatgtgc atgattccta catagcagt
//
LOCUS       DBMDXG010000205         3630 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5879_length_3630_cov_1.806177, whole genome shotgun sequence.
ACCESSION   DBMDXG010000205 DBMDXG010000000
VERSION     DBMDXG010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5879_length_3630_cov_1.806177"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(134..1059)
                     /locus_tag="ACZPD3_06475"
                     /pseudo
     CDS             complement(134..1059)
                     /locus_tag="ACZPD3_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(1377..1694)
                     /locus_tag="ACZPD3_06480"
     CDS             complement(1377..1694)
                     /locus_tag="ACZPD3_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415678.1"
                     /translation="MWLVTALEQTAPEILWEMMNRRWDIEENGFHQLKTYYYAKHCYC
                     HAAVEVIFNLMIIGFNMRELYLYRRIQKFARSRITRKSVSRIFRDDLLTEKVKQILYQ
                     KGG"
     gene            2280..2687
                     /locus_tag="ACZPD3_06485"
     CDS             2280..2687
                     /locus_tag="ACZPD3_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415679.1"
                     /translation="MKKRIKLISLAVAAGMLISATNVSAYTRQYTFHMNTSYAHGKDP
                     NCGYAATKDDNVAIAYITMTQYKINSGTPQISMWVAPSGTYAQATDAWTWTGTAPRKS
                     LGYNRAVKKGMAFQLCAEQFGSGRAVIGGRWTP"
     gene            2867..>3630
                     /locus_tag="ACZPD3_06490"
     CDS             2867..>3630
                     /locus_tag="ACZPD3_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415680.1"
                     /translation="MKNIIRIISLIFVVTAIGGCGKSQQYQESTDFPKGMKTELTEQI
                     KVNAEFQYPENCKNGKCAETQISGQTLWDKQEEIAQLLVQDGNITNEYIDDYGTVQYK
                     IYEIAENNATLVISDDNLNYATDQASYINNVLFSDSRFDDYNGNKYQDKTDLPFMPQK
                     EAWKNVSEVLDKLGVDVSDDVICYVMEHSIMSEEEKKAIARAEEEDTKIPTAKTQWTE
                     DDDCYYFMTYTTWQDYPVLPPVMGEGFDENNVSVIY"
BASE COUNT         1117 a          641 c          790 g         1082 t
ORIGIN      
        1 acaacgaatg gtggtgtggt acaatgctgt agaaagaaga atgctggaaa gccgtatagt
       61 cgtatgaagg agaaactcac atgcagtttg atgagaggaa agagggaata gaccctctga
      121 cctactctac atattattct gtctgcttct gtggaagctg tgcaaggatg atagcagcaa
      181 acatgatgac acatccaagg atttctttag tacttagttg ctgtccgagg atgatccatc
      241 cggctaggac agagatgcag gattccaggc tgagtatcag ggatgctaca gttgggttca
      301 tgtttttctg accgataatc tgcaaggtgt aagctactcc gcatgacatg acacctgcgt
      361 agaggattgg ctgccatgct gtcaggatgg atgagagttc cggatgctca aggagcagag
      421 cgggaatgcc ggaaagaact ccacatgtca gaaactgaat acaggagagc tttactccgt
      481 ctactttcgg agaaaagtag tcgatgacca gtatgtgcaa cgaaaaaaga aaagcgcata
      541 ccagtacgag cagatctcct tttccaatgg aaaatccatc tgtgatgcac aggagataaa
      601 gtccgatcag agccattgca acggcagccc agataaattt cgtgcatttt ttgccaagga
      661 acagtccgat gacagggacg atcacaatat aacaggcagt gataaagccg gcttttccga
      721 cggttgtata cttgatgcca aactgttgga aattgcttgc cagacagagg cagataccgc
      781 aggcgatacc gccgataatc agagtttttt ctcctgcata ttggatacag tattacttga
      841 ggtgtcagat ctgccagatg ctttggaagc agaatctgaa ggagagtttc tctttttcag
      901 aataaaaatc agtggaatca gaacgactgc tccgataagg gaacggacgg cattaaaagt
      961 aaagcctcct acgtatttca ttcctacgct ttgagccaca aaagcgatac cccagatgat
     1021 cgctgcgaga agcaggagca gggaattttt taatggaatc tgttgttttt gcatgatgtg
     1081 tgtaatcctt ccttatattt caaatatatt ttaagagcgc ctgtgcttca ttgatctgaa
     1141 attgaataaa cactttcata attatacatc atagatgtaa aaagagaaag acgattatag
     1201 aaataacaga gatttcgctt ttagtttttt ggttcatgca tttttataaa aaatatgttc
     1261 ctctgacatt ctgctcgcag ctgttactct ttttgacccc aatgcgcaca aagttcccct
     1321 ctgcttcgtc tctgtctttt tttcttatat tatttttgat ctcttttata cagatcttat
     1381 ccaccttttt gatacaggat ctgttttact ttctctgtca gcagatcatc tctgaatatc
     1441 cggcttacgc tctttcttgt gatcctgctc cttgcaaact tctggatccg ccgatacagg
     1501 tacagctccc tcatgttgaa ccctatgatc atcagattaa atataacttc taccgccgca
     1561 tggcagtaac agtgctttgc atagtagtac gtcttcagct ggtggaaccc attctcttcg
     1621 atatcccacc tgcggttcat catctcccat aagatctccg gggctgtctg ttccagcgct
     1681 gttaccagcc acatccatct ctccgcttcc ttcccgttct cctgccacat ttcttagtct
     1741 tttggacatg ctttccggtg cggaaaaaat agtgtgaaaa ctttcatact gtagcatcag
     1801 aaagagcagc accggcataa caatgttaaa cagcggaatt gatttccttt ttcttttatc
     1861 cgataagcac ttgatttttt gcgggatttt atatacactt gttatataag ttaacagttt
     1921 ttttaatgct aatttttcat taaggacgcc ttctttcgta tgttttgttg gtaacataat
     1981 tatacatgaa aaggtgtcct ttttgattgt ttatacataa aaaaatgtaa ctttttattc
     2041 catatggttc tttacggttt tctgcataaa ctgttggtaa aactggcatc ttttttctta
     2101 aaagcgaaat ctctgaatat ataaactaaa tattgacaaa tatgcgaata aatgataaat
     2161 taaatgcatg acatgaatgt agacaatgta gaatgtgagg tggtgagaat agtaagaatt
     2221 ttgcattaag gttttagcaa aaattaatct ggcgattgta atgaaaggag gaagtataat
     2281 tgaagaaacg tataaaacta attagcctgg cagttgcagc agggatgttg atatctgcaa
     2341 caaatgtttc ggcatataca cgtcagtata catttcatat gaatacgtct tatgcacatg
     2401 gaaaagatcc taactgtggt tatgctgcga cgaaggatga taatgtagca attgcctaca
     2461 taactatgac tcaatacaag ataaatagtg gtactcccca aatttctatg tgggttgccc
     2521 caagcggaac atatgctcag gcaaccgatg cctggacatg gactggaaca gctcctcgca
     2581 aatcattagg gtacaacaga gctgttaaaa agggaatggc atttcagtta tgcgccgaac
     2641 agttcggaag tgggagggct gtgattggag gaagatggac tccttaactt tttcagaccg
     2701 cagatttgat gagtgttaga aaaatgtttg tatgctgaca tagatatcac aataattcct
     2761 ttttggatgc aggttataac tttctcggat tttgttatac tgtatctgga gaggaattat
     2821 ttggataagt tttgtgattg tgtggttggt aagatgagag ggacatatga aaaatattat
     2881 aagaataata tctctgatat ttgtagttac agcgattggt ggatgcggta agtctcaaca
     2941 gtaccaggaa tcaacagatt ttccgaaagg catgaaaacg gaactgacag aacagataaa
     3001 ggtaaatgca gagtttcaat atcctgaaaa ctgtaaaaat ggaaaatgtg cggagacaca
     3061 gattagtgga cagacactct gggataagca ggaagaaatt gcacaattgt tggttcagga
     3121 tggtaacatt acaaatgaat acatagatga ttatgggaca gtacagtata aaatttatga
     3181 aatagctgaa aacaacgcaa ctttagtgat ttctgatgat aatttgaatt atgcgactga
     3241 tcaggcttct tatataaata atgtactgtt ttctgacagt cgttttgatg attataatgg
     3301 aaataaatat caggataaaa cggatcttcc ttttatgcct caaaaagaag catggaagaa
     3361 tgtaagtgaa gtgctggata aactgggggt tgatgtatca gatgatgtta tatgctatgt
     3421 tatggaacat agtattatga gcgaagaaga aaaaaaagcg attgccagag ctgaggagga
     3481 agacacaaaa attccgactg caaaaacaca gtggacagaa gatgatgact gttattattt
     3541 tatgacttat actacatggc aggactatcc ggtattaccg cctgtaatgg gagagggatt
     3601 tgatgagaat aacgtatctg ttatatacga
//
LOCUS       DBMDXG010000206         3626 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5888_length_3626_cov_1.756596, whole genome shotgun sequence.
ACCESSION   DBMDXG010000206 DBMDXG010000000
VERSION     DBMDXG010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5888_length_3626_cov_1.756596"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="ACZPD3_06495"
     CDS             <1..1615
                     /locus_tag="ACZPD3_06495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415681.1"
                     /translation="ISDAMYYSVIKNTDIGSESLEDAMTLLYEANKDKLISVACYTND
                     GKLTEASPIATEKNGVDVKSQKWFQDAAGELENFHFSTPHVQNLFDDPSYRYYWVVSL
                     SRTVELTRNGSSMQGVLLVDMNYSSIEQLLEKANTDTSGEYVYLMASDGEIIYHPKQN
                     LIPMGLYQENNDKAAGYEDSTVKENFHGEKRLVTVKTISYTGWKLVSVVPMKSFSMGM
                     TGMRNLVVLLVALTVLAVVILNQMVSARISKPLRRLNNSVKEWETGNMDPDIYIGGSM
                     EVEHLGKTLRSTVAQIRQLMDDIVVEQEEKRKSELDALQSQINPHFLYNTLDSIVWMI
                     TGERYDDAVFMITQLASLFRISLSKGKTVIRIEDEVKHAQNYMNIQKIRYKNSFEIDF
                     QIEEEILDGCIVKLVLQPLLENAIYYGMEFMDGEGEIHVRGYRKDGDIYLEVEDNGLG
                     MPEEEAAELLNGKERPHKHGSGVGLVNVHSRLKLRFGEGYGLIIHSCPDEGMLVQIHI
                     PYVPYTAETQKLLESGKDYHLADRKGDAE"
     gene            1612..2562
                     /locus_tag="ACZPD3_06500"
     CDS             1612..2562
                     /locus_tag="ACZPD3_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024799.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1415682.1"
                     /translation="MKKNRISAILFLAVILSVIIYSAYGMVNQGKDDDYISVSVIVND
                     SSNTRWNAFREGLEQGAEDNQVHLNLVSTGSFQNVQEENTIVKRELEGGADGIIVEPY
                     SGEADSVLQGVAADSVVLVKTGIETDTLYTSVMTDHIKFGESLADAVIEAGETRVGIF
                     SGNQSQLGMRQRLKGVKERLEEAGVEISWVFSENELNKKIRNAEYFVNNQVNAVISLE
                     NDDTEKAVEMLFDNEGVSWRIYGEGRSEKTVYYLDKGLIRELVVPNEYYMGYESMVLA
                     AQMARYHTDKMEQVEVDFFRVTKENLYAEETSQILFPTVR"
     gene            2584..>3626
                     /locus_tag="ACZPD3_06505"
     CDS             2584..>3626
                     /locus_tag="ACZPD3_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1415683.1"
                     /translation="MDGGKEMCRKYLVLAGVLCIVAVNVGGCKSSEEETIKNIKIGVT
                     LYDQYDTFLSEMMAEFTKYTADKEEESGVAINMEIMDASQSQLTQNEQVKSLIDKGCD
                     VICVNLVDRTEPTTITDLAENNNVPIIFFNRELVAEDLERWTELYYVGADASESGTLE
                     GELAADAFKTDSSMDKNGDGICQYVVLEGEAGHQDSIVRTEYSVNTMAEKGVETEKLG
                     YAIANWNRAQAQTKTAAMLTQFDNKIELIIANNDDMALGAIDALKASQIPKEEWPGVV
                     GIDGTDVGLNAVKNEEMLGTVYNDKEGQAQEMLNLAFAIATNGKTDEIPLLNGKYVRI
                     PYHKVTLDNVEEY"
BASE COUNT         1167 a          567 c          980 g          912 t
ORIGIN      
        1 tatttcagat gccatgtatt atagtgtgat caagaataca gatattggaa gtgagagcct
       61 tgaggatgcc atgactcttt tatatgaggc taataaagat aagctgatca gtgtggcatg
      121 ttacacaaat gatggaaagc tgacggaggc gtctcctatt gcaacagaga aaaatggtgt
      181 tgatgtaaag tcgcagaaat ggtttcagga tgcagccgga gaactggaaa attttcattt
      241 ctccacaccc catgtgcaga atctctttga tgatccatct tacagatatt actgggtggt
      301 gtctttgagc cgtacggtag agctcaccag aaatggcagt tccatgcagg gcgttcttct
      361 ggttgatatg aattacagca gtattgagca gcttcttgag aaggcaaaca cagatacttc
      421 cggtgaatat gtatatctga tggcatctga cggagaaatc atctatcatc cgaagcaaaa
      481 tcttatccct atgggattat atcaggaaaa taatgataag gcagcaggat atgaggattc
      541 tactgtaaag gaaaattttc atggtgaaaa gcggctggtt acagtaaaga ccatcagtta
      601 tacaggatgg aaactggtca gtgtggtccc tatgaaatct ttcagtatgg gaatgaccgg
      661 tatgagaaat ctggtagttc ttctggtggc tctgacagtg ctggcagtgg tgatactgaa
      721 tcagatggta tctgcaagaa tatcaaaacc attgcgccgt ctgaataatt ctgtgaagga
      781 gtgggaaacc ggaaatatgg atccggatat ttatatagga ggttccatgg aagtagaaca
      841 tctgggaaaa actctccggt ctacagttgc ccagatccgg cagctgatgg atgatattgt
      901 cgtggaacag gaagagaaac ggaagagtga gcttgatgca ctgcagtcgc agatcaatcc
      961 gcatttctta tataatacac tggattctat tgtctggatg atcacgggag agcggtatga
     1021 tgatgcagta tttatgatta cacagcttgc cagtctgttc cgtatcagtc tgagtaaagg
     1081 aaaaacagtg atcaggatag aagatgaagt aaagcatgca cagaactata tgaatatcca
     1141 gaagatcaga tataagaaca gttttgaaat agattttcag attgaggaag agatactgga
     1201 tggctgtatt gtgaagctgg ttctccagcc attacttgag aatgccattt attatggaat
     1261 ggaatttatg gacggagaag gagagattca tgtccgcggt tacagaaagg atggggatat
     1321 ttatctggaa gtagaggaca acggactggg tatgcctgag gaagaagcag cagaacttct
     1381 caacggaaaa gagcggccgc ataaacatgg atcaggagtt ggtctggtta atgtgcacag
     1441 ccgtctgaag cttcgtttcg gagaaggata tggactgatt attcatagct gtccggatga
     1501 gggaatgctg gtgcagattc acattccata tgttccgtat acggcagaaa cccagaaact
     1561 ccttgagagc ggaaaggatt atcatttagc agacagaaag ggggatgcgg aatgaaaaag
     1621 aacagaatat ccgcaatcct cttcctggca gtgattttat ctgtaatcat atacagtgct
     1681 tacggaatgg tcaatcaggg aaaagatgac gattatattt ctgtatcagt gatcgtaaat
     1741 gacagcagta ataccagatg gaatgcgttc cgggaaggac tggaacaggg agctgaggat
     1801 aatcaggtac acctgaatct ggtgtcgaca ggaagttttc aaaatgttca ggaggagaat
     1861 acgattgtaa agcgtgaatt ggaaggaggg gcagatggga taatcgttga accctattct
     1921 ggcgaagctg acagtgtttt gcagggagta gctgcagact cggtagtatt ggtgaaaacg
     1981 ggaatagaaa cagatacact ttatacttct gtaatgacag atcatataaa attcggagaa
     2041 agtctggcag atgcggtaat tgaagccgga gagacaaggg ttgggatttt cagtggaaat
     2101 cagagtcagc ttggaatgag acagcgtctg aagggagtaa aggaacgtct ggaagaagca
     2161 ggagtggaga tttcctgggt gttttctgaa aatgagctta ataagaaaat cagaaatgca
     2221 gagtattttg tcaataatca ggtgaatgca gtaatttcgc tggagaatga tgatacagaa
     2281 aaggcagttg agatgctctt tgacaatgaa ggggtttcct ggcggatcta tggagaagga
     2341 cgctctgaaa aaacggtata ttatctggat aaagggctga tccgggagct tgtagttccc
     2401 aatgaatatt atatgggata tgagagtatg gttctggctg cacaaatggc aagatatcat
     2461 actgacaaaa tggaacaggt agaagttgat tttttcagag taacaaaaga gaatctgtat
     2521 gcggaagaga caagtcagat tctctttcca actgtaagat aatgttatac gaaaacagat
     2581 aagatggacg ggggaaaaga aatgtgcagg aaatatctgg tgctggcagg tgttttatgt
     2641 attgtggctg ttaatgtcgg aggatgtaag agcagtgaag aagagacaat caaaaatata
     2701 aaaataggag ttactttata cgatcagtat gacacattcc tttcggaaat gatggcggaa
     2761 ttcacgaaat atacagcaga caaagaagag gaaagcggcg tggcgatcaa catggagatc
     2821 atggatgctt ctcagagtca gctgacgcag aatgaacagg ttaagagcct gatcgataaa
     2881 ggatgcgatg tgatatgtgt aaatctggta gaccgtacag agcctaccac aatcacggat
     2941 cttgcagaga ataataatgt tcctattata ttttttaacc gtgaactggt tgcagaggat
     3001 ctggaacgct ggacggaact gtattatgtg ggggcagatg cttcagagtc aggaacactg
     3061 gaaggggaac tggcagcaga tgcttttaag acagattctt ctatggataa aaatggagat
     3121 ggcatttgcc agtatgtagt gcttgagggc gaagccggac atcaggactc cattgtgcgc
     3181 acagaatatt ctgtaaatac catggctgaa aaaggtgtgg aaacagagaa actgggatat
     3241 gccattgcca actggaacag agcacaggcg cagaccaaaa cagcagccat gctgactcag
     3301 tttgacaata agattgaact gatcattgcg aataatgatg atatggcgct gggtgcaatc
     3361 gatgccttaa aggcatctca gattccaaaa gaggaatggc ccggtgtagt ggggattgat
     3421 ggtacagatg tagggctgaa tgcagtaaag aatgaagaga tgcttggaac cgtctataat
     3481 gataaagaag gacaggcaca ggaaatgctg aatcttgctt ttgcgatcgc cacaaatgga
     3541 aaaacagatg agattccgct gcttaatgga aaatatgtac ggattccgta ccataaggtt
     3601 acattggaca atgtagaaga atatga
//
LOCUS       DBMDXG010000207         3612 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5910_length_3612_cov_1.671888, whole genome shotgun sequence.
ACCESSION   DBMDXG010000207 DBMDXG010000000
VERSION     DBMDXG010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5910_length_3612_cov_1.671888"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="ACZPD3_06510"
     CDS             complement(<1..832)
                     /locus_tag="ACZPD3_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415684.1"
                     /translation="MKILVAVLALGLAFWGYAFVYRGKTEPPAKYPVTNQDAAVYSLR
                     GQIQMLSQQEELNTFAFEGRKKEKEYGTYIIPGLRYTRTFLNAEGTKQAVCTSMTPQG
                     LAVTDEYVLVSAYCHTEKHNSVIYVINKETHRFIKEVVLPGLPHVGGLAYDQEHDMLW
                     YSSNTNGIAQAISIKMDVLRDYNYADNHMPVQVNQTCSLYGIVRDSFMTFYKGCLYVG
                     CFNKYTESTIARYAVDSKGDLVNTFNEELGMMFEMAVPLDYSTISEQAQGMAFYNNKL
                     L"
     gene            complement(875..1426)
                     /locus_tag="ACZPD3_06515"
     CDS             complement(875..1426)
                     /locus_tag="ACZPD3_06515"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR1415685.1"
                     /translation="MNKGIIYCTKIREEYEGAHMEHMIAEKLLEIALKKEYGINLYNE
                     PRAEGEHGKPFLSYRPSLHYNISHSGKYVVCILADQEVGIDVQIHCRANYERMLRRMV
                     PREQYLEILSDINVEKKFFEQWVLREAYIKWTGEGLSRDMRTISMDEGSHMLLDMEDG
                     YSGAVWAMNPMEINWKYEDIILG"
     gene            complement(1423..3249)
                     /locus_tag="ACZPD3_06520"
     CDS             complement(1423..3249)
                     /locus_tag="ACZPD3_06520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1415686.1"
                     /translation="MKRNPSQLRAITHLSGPMMVLAGPGSGKTSVIVERTAYMINEGK
                     IPASSILVVTFSRAAATEMKERFLKFVGQNRSEVTFGTFHGIFYGILKAAYHLSAANI
                     LSEEEKFSILREMTEKYGQEMAQEGDFIEEVAREISVVKGNCISPEHYYASCCSDEIF
                     RDIFHGYKQALKAKRKLDFDDMILCCYELFSQRQDILNAWRRKFVYILVDEFQDINSL
                     QYKILQMLAAPVNNLFIVGDDDQSIYHFRGARPEIMLNFTKDYPKAETVLLNVNYRCS
                     KNILRTAMEVIGCNTRRFKKQLDTPNEEGMPVTCKEFDNPREEYMCVVAALKKRMERG
                     EDLLNTAILLRTNQESEGLINVLMEYQVPFTMKEQLPNLFRHWICRALLAYLEMAAGD
                     RSRKNFLEIMNRPNRYISREALNSSQINFNELREYYKDKDWMCDRITTLETHLRILGT
                     LSPFAAINFIRKGMGFEEYLREYAQYRKIKPEELLETLDRIHESAKGMKTLAKWQAYI
                     EEYTKRLNEQARKQQDKKEGVTISTLHAVKGLEYDIVYILNVNEGSIPYRKAVLAEAV
                     EEERRLFYVGMTRAKKKLVLAYVKRQYEKEREPSRFLEETGL"
     gene            complement(3252..>3612)
                     /locus_tag="ACZPD3_06525"
     CDS             complement(3252..>3612)
                     /locus_tag="ACZPD3_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR1415687.1"
                     /translation="ETFPDIPALIDFFEEVPEYDSAMYCHKKMKTNEETSLTVLKEVL
                     PVLEEQEDYTNDPLFETLSAFVKEHGYKNGYVMWPLRTAVSGKQMTPAGATEIMEIIG
                     KDETIKRVKAAIEKLSK"
BASE COUNT          930 a          912 c          630 g         1140 t
ORIGIN      
        1 acaacagctt attgttataa aaagccattc cctgtgcctg ttctgatatt gtagaataat
       61 ccaacggaac cgccatttca aacatcatgc caagttcctc attaaaagta ttcaccagat
      121 caccttttga atccaccgca tatctcgcga tggtgctctc tgtatatttg ttaaaacatc
      181 ctacatacag gcacccctta tagaaagtca taaaagaatc tctcacgatt ccataaagac
      241 tacatgtctg atttacctgc acaggcatat ggttatccgc ataattatag tcacgcagca
      301 catccatctt aatgctgatc gcctgcgcga ttccattggt atttgaagaa taccacagca
      361 tatcatgttc ctgatcatag gcaagtccgc ctacatgagg cagacctggc aggactacct
      421 ctttgataaa tctgtgagtt tctttgttga tcacataaat aacagagtta tgtttttccg
      481 tatgacaata ggcacttaca agtacatact catcggtcac tgcaagtccc tgaggagtca
      541 tggaagtaca taccgcctgt tttgtaccct ctgcattcag gaatgttctt gtatatctca
      601 gtccgggaat aatataagtt ccgtactctt tttctttttt tctgccctca aaggcaaagg
      661 tatttaactc ctcctgctgc gacagcatct gtatctgtcc acgtaatgaa tacactgcag
      721 catcctggtt ggttaccgga tattttgcag gaggttccgt cttacccctg tatacgaaag
      781 catatcccca gaaagccagt ccaagtgcca gtactgctac aagaatcttc actgccagtc
      841 tccggatttc tttcttcgtt tgtctgttca tgtcttaccc aagtatgatg tcttcatatt
      901 tccagttgat ttccatggga ttcattgccc atactgctcc actgtaaccg tcttccatat
      961 caagcagcat atgactgcct tcatccatgg atatagtcct catgtctctg gaaagcccct
     1021 caccggtcca tttgatataa gcttccctca gcacccactg ttcaaaaaat ttcttttcca
     1081 catttatatc tgaaagaatt tcgagatact gttcccgggg taccatacgt ctgagcattc
     1141 tttcataatt ggctctgcaa tgaatctgca catcaattcc aacctcctga tcagccagaa
     1201 tacataccac atattttccg gaatgactga tattataatg cagggacgga cgataggaaa
     1261 ggaatggttt tccatgttct ccctctgctc tgggttcatt gtacagattg attccatatt
     1321 cttttttcag tgcgatttcc agaagtttct ctgcgatcat atgctccata tgtgcgcctt
     1381 catattcttc ccgtattttt gtacagtaaa taattccttt attcataggc ctgtctcctc
     1441 aagaaaccgg gaaggctccc gttccttctc atactggcgt ttcacatagg caagcaccag
     1501 cttctttttc gccctggtca tccctacata aaacaatctt cgttcttctt ccactgcttc
     1561 tgccaaaacc gccttgcgat atggaatgct tccttcatta acattcagaa tatacacaat
     1621 atcatattcc agacctttta ccgcatggag tgtagatata gtcactcctt cttttttatc
     1681 ctgctgtttc cttgcctgtt cattcaggcg cttcgtatat tcttctatgt aagcctgcca
     1741 cttcgccagg gttttcatgc cttttgcact ctcatggatc cggtccaggg tctcaagcag
     1801 ttcctctggt tttattttac gatactgtgc atattcacgc aagtattctt cgaatcccat
     1861 tccttttctg ataaaattaa tcgcagcaaa aggggaaaga gtaccaagaa tcctcagatg
     1921 cgtttccagt gtggtaatcc tgtcgcacat ccaatctttg tctttataat attcccgaag
     1981 ttcattaaaa ttaatctgcg aactgttcaa cgcttctctg gaaatatagc gatttggccg
     2041 gttcatgatc tccagaaaat tcttcctgct cctgtctcct gctgccattt caagataagc
     2101 cagaagcgcc cggcagatcc agtggcggaa aagattggga agctgttctt tcattgtaaa
     2161 aggaacctga tattccatca gcacattgat cagaccttcc gattcctgat ttgtacgaag
     2221 caggattgcc gtattcagca gatcctcgcc gcgctccatt ctttttttca gtgctgccac
     2281 cacgcacata tactcttctc ttggattatc aaattccttg cacgtcacag gcatcccttc
     2341 ttcatttgga gtatccagct gctttttgaa acgtctggta ttacatccga tcacttccat
     2401 ggctgtacgc aggatattct tactgcaccg gtaattcaca ttcagaagga ctgtctctgc
     2461 cttcggataa tcctttgtaa aatttaacat aatctctggt cttgcgcccc ggaaatgata
     2521 gatggactgg tcatcatccc ctacaataaa caggttattg acaggtgccg ccagcatctg
     2581 caaaatcttg tattgtaagc tgttgatatc ctgaaactca tccaccagaa tgtagacaaa
     2641 ttttcttctc catgcattca ggatatcctg tctctgagaa aaaagttcat aacaacagag
     2701 gatcatatca tcaaaatcca gcttgcgttt cgccttcaga gcctgcttat agccatgaaa
     2761 aatgtccctg aatatctcat ccgagcaaca ggatgcataa taatgctccg gagaaataca
     2821 gttccccttt acaacactga tttccctggc aacttcttca atgaaatctc cctcctgagc
     2881 catttcctgg ccatatttct cagtcatctc acgtagaata ctgaattttt cttcctcaga
     2941 aagaatattg gccgcactca gatgataggc cgctttcaaa atcccataaa aaattccgtg
     3001 gaaagttcca aaagtcactt cgcttctgtt ctgtcccacg aatttcaaaa accgttcttt
     3061 catctctgtt gccgctgctc tggaaaaagt tacaaccaaa atagaagaag ccggtatctt
     3121 cccctcattg atcatgtatg cagttctctc cacaataacg gaagttttcc ccgagccggg
     3181 acctgccagg accatcatag gtcctgacag gtgagtgatt gcccgaagtt gagatggatt
     3241 tctcttcata tttatttgct taatttctca atagctgcct ttactcgttt gattgtctcg
     3301 tctttaccga tgatctccat gatctcagta gcaccggccg gcgtcatctg cttgccggaa
     3361 acagcagtac gcagaggcca cattacatat ccgttcttat aaccatgctc ttttacaaat
     3421 gcacttaatg tttcaaataa cggatcattt gtataatctt cctgttcttc cagaaccgga
     3481 agtacttctt ttaatactgt aagagaagtc tcttcatttg tcttcatttt cttatggcag
     3541 tacatggcgg aatcatattc cggtacttcc tcaaagaagt caatcagagc cggaatgtca
     3601 gggaatgttt cg
//
LOCUS       DBMDXG010000208         3611 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5913_length_3611_cov_1.549573, whole genome shotgun sequence.
ACCESSION   DBMDXG010000208 DBMDXG010000000
VERSION     DBMDXG010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5913_length_3611_cov_1.549573"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(30..398)
                     /locus_tag="ACZPD3_06530"
     CDS             complement(30..398)
                     /locus_tag="ACZPD3_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR1415688.1"
                     /translation="MKSYTITVNGTAYEVTVEETGSVSAPAAAPKAAPKAAPAAAPKA
                     AAPAAGAGAVKVTASVPGKVVKVAASVGQAVKAGDSVIILESMKMEIPVVAPQDGTIA
                     SIDVAEGASVENGDTLATMN"
     gene            complement(426..1181)
                     /locus_tag="ACZPD3_06535"
     CDS             complement(426..1181)
                     /locus_tag="ACZPD3_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HIR1415689.1"
                     /translation="MKMIKKITSVFMTFLVMAALVIGSTSVVFAADKVDDTVKSTLVT
                     TAEGLTDAIVQLKDEEIENYMSSGDDFTTSAMQSWQTSKDELGAKKDSNGETTVTFKD
                     DQYTVTVPLKFEKAEANFVYVFDSQGTPTSMSVDVQYGMGKTLQRAGLNTLMGIGTVF
                     VMLILLSLLISLFRFIPNPEAKKAAEAKAAKAAKEAEAAAIAQTAPAQAEENLADDGE
                     LVAVIAAAIAAAEGTTTDGFVVRSIRKVKRNRR"
     gene            complement(1196..2632)
                     /locus_tag="ACZPD3_06540"
     CDS             complement(1196..2632)
                     /locus_tag="ACZPD3_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR1415690.1"
                     /translation="MSNATQSLAGTRITSLLDANSFVEIGQGVTARSTDFNMTANKAP
                     SDGVITGYGVIDDKLVYVYSQDASVLNGTVGEMHAKKITRLYDLAMKTGAPVIGLVDC
                     AGIRLQEATDALEAFGQIYLKQTLASGVIPQITAIFGTCGGGMAVIPSLTDFTFMESK
                     KGKMFVNTPNALEGNNTDKCDTAAADFQSKETGVIDGVGTEDEILGQIRSLVSLLPSN
                     NEDTDNYTECTDDLNRVCADLANCAGDTAIALSQIADNGEFFETKADYAKDMVTGFIR
                     LNGATVGAVANRSEVYDAEGKKTETFDGSISARGARKAADFVKFCDAFDIPVLTLTNA
                     TGFMATLCSEKMMAKSVGELVAAFADATVPKVNVIIGKAYGTAYVAMNSKSIGADLVY
                     AWDNAEIGMMDASLAAKIMYADADAAELNEKAAQYRELQNGVASAAARGYVDTVISPA
                     DTRKYVIGAFEMLFTKREDRPSKKHGTV"
     gene            3192..>3611
                     /locus_tag="ACZPD3_06545"
     CDS             3192..>3611
                     /locus_tag="ACZPD3_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR1415691.1"
                     /translation="MKSNYQHIFTLLTVKNMTIKNRIVMMPMGTNYGEQNGEMSFLHI
                     NYYKERAKGGTGLIIVENASVDSPQGSNGTTQLRIDHDNYLPRLYKFCEEIHKYGTCI
                     AIQINHAGASAVSARTNMQPVSASDVPSKEGGEIPRPL"
BASE COUNT          913 a          850 c          714 g         1134 t
ORIGIN      
        1 ttacataaga catttgacta cctcccgtct tagttcattg ttgctaaagt atctccgttt
       61 tctacagagg caccttctgc aacatcaatg cttgcgatag taccgtcctg aggagctact
      121 acagggattt ccattttcat ggattcaaga ataattacgc tgtctccggc ttttactgcc
      181 tgtccaacgc ttgctgctac tttgacaact ttaccaggta cggaagcagt tacttttact
      241 gcgccggcgc ctgctgcagg agctgctgct ttcggagctg ctgcaggtgc agccttaggt
      301 gctgcttttg gagctgctgc aggagcggat acgcttcctg tttcttcaac agttacttca
      361 tatgctgtac catttactgt aattgtatag cttttcattt taaaatcctc ctaaataaat
      421 gacaattatc ttctatttct ttttacttta cggatggaac gaactacgaa gccatctgtt
      481 gtagttcctt ctgctgctgc gatagctgct gcgataacag ctaccagctc tccgtcatct
      541 gcaaggttct cttctgcctg tgcaggtgct gtctgtgcga tggctgctgc ctctgcctct
      601 tttgcagctt tggctgcttt tgcttctgct gctttctttg cttccggatt cggaataaat
      661 ctaaacagag aaatcagaag gcttaataaa atcagcatta cgaatacagt tccaataccc
      721 atcagagtat taagacctgc tctctgaaga gtcttgccca taccatactg tacatctaca
      781 gacatggaag tcggtgttcc ctgtgagtca aagacatata caaagtttgc ttctgctttc
      841 tcaaatttaa gaggaacagt aactgtgtac tgatcatctt tgaaagttac agtagtttca
      901 ccattgctgt ccttctttgc gccaagttca tccttggatg tctgccagga ctgcatggca
      961 cttgttgtaa agtcatctcc tgaactcatg taattctcga tttcttcatc tttaagctgg
     1021 acaatggcat ctgtcagtcc ttctgctgta gttaccagtg tggacttaac agtatcatca
     1081 accttatctg cggcaaatac cacagaggtg ctgccgatca ccagcgcagc cattacgaga
     1141 aatgtcatga atacagaagt tatttttttg atcattttca tatgcgtcac cccgcttaga
     1201 ccgttccatg tttcttagac ggacgatctt ctctctttgt gaacagcatc tcaaatgcac
     1261 cgattacata ttttcttgtg tcagccgggc tgatcacagt gtcaacatag cctcttgctg
     1321 ctgcggaagc aacaccattc tgaagctctc tgtactgagc agctttctca ttcagttcag
     1381 cagcatctgc atctgcatac atgatcttgg ctgccaggga agcatccatc ataccgattt
     1441 ctgcgttatc ccatgcatat acaaggtctg caccgataga tttgctgttc attgccacgt
     1501 aagctgtacc gtaagctttg ccgatgatca catttacctt cggaacagtt gcatctgcga
     1561 atgcagctac cagttcacct acggatttcg ccatcatctt ctcgctgcat aaggttgcca
     1621 tgaagcctgt tgcgtttgta agtgtaagta ccggaatatc aaaagcgtcg cagaatttca
     1681 cgaaatctgc tgcttttctt gcgcctcttg cagaaatgct tccgtcaaat gtttctgttt
     1741 tcttaccttc tgcgtcatat acttcgcttc tgtttgcaac agcacctact gtagcgccgt
     1801 ttaaacggat aaagcctgta accatgtctt ttgcatagtc tgcttttgtc tcgaagaatt
     1861 caccgttgtc agcgatctgg gaaagtgcga ttgctgtgtc acctgcacag tttgcaagat
     1921 ctgcgcatac gcggttaaga tcatctgtac actctgtgta attgtctgtg tcttcattat
     1981 tagaaggaag aagtgatact aaagaacgga tctggccaag aatctcatcc tctgtaccaa
     2041 cgccatcgat cacacctgtc tctttgctct ggaaatcagc tgctgctgtg tcgcatttgt
     2101 ctgtgttgtt tccttccagt gcgtttggtg tgttgacgaa catttttcct tttttggatt
     2161 ccatgaatgt gaaatctgtc agggacggga taactgccat accaccgccg catgttccga
     2221 agattgctgt gatctgcggg atcacaccgg atgccagtgt ctgtttcaga tagatctgtc
     2281 cgaaagcttc cagtgcgtct gttgcttcct gaagacggat acctgcacag tcaacaagtc
     2341 cgataaccgg agcgcctgtt ttcatggcaa gatcataaag tcttgtgatc ttctttgcat
     2401 gcatttcacc aacagtaccg tttaatacag atgcatcctg gctgtataca taaaccagtt
     2461 tgtcatcgat cacaccatat ccggtgataa caccgtcaga tggtgccttg tttgcagtca
     2521 tgttgaaatc tgtggatctg gctgttacac cctgtccgat ttcgacaaaa ctgtttgcgt
     2581 caagcaaaga agtaattctt gtgcctgcta agctttgtgt tgcattactc attcttgttt
     2641 gccctccatc tttatgagaa tatttaaaag ttaacccaaa aatttagccg attttaattt
     2701 taccttttcc ggcagaaaat ttcaacattt atttgtcatt ttcgttaaaa atatagcaga
     2761 aaaatttgtc aggtttctcc aaattttgtt tttttgagca tttcttccta tttaatagag
     2821 ggtattacgg ttattttgta ttgtcccgta tttcaccctc tcccgcaaac cagtgtatcg
     2881 acaagttgat ttctcgaaca atcctgcgtc tgaatttctt atttctatcc tgcaagactg
     2941 tttcaagaat caacttgtcg gcacaccaga gcgtgtttga ggtaactata ccactcactt
     3001 gtctgcgaat ccgactgcac aggtataaaa gtctcagcaa aaccatagat gttttttatt
     3061 atatagcacg aattatcaat tagatttatg gattatccca ttatataatt aacacatcaa
     3121 agaaattcag gccacaacta aagcactgaa caattataga ttcaacagaa aatttcagga
     3181 ggaaagacgt aatgaaaagc aattaccagc atattttcac actactcaca gtaaaaaata
     3241 tgactatcaa aaaccgtatc gttatgatgc ctatgggaac caattacggg gaacagaacg
     3301 gcgaaatgag ttttcttcac attaattatt ataaagagcg tgccaaaggc ggcacaggtc
     3361 tgatcatcgt agagaatgca agcgtagatt ctccgcaggg ttccaacgga actactcagc
     3421 ttcgtattga ccacgacaac tatctgccgc gtctgtacaa attctgtgaa gagatccaca
     3481 aatatggtac ctgtattgcg atccagatca accacgcagg tgcttccgct gtttccgcac
     3541 gtacaaatat gcagccggtt tccgcatctg atgttccgtc caaagaaggc ggagaaatcc
     3601 cgcgtccgtt a
//
LOCUS       DBMDXG010000209         3597 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5934_length_3597_cov_1.163902, whole genome shotgun sequence.
ACCESSION   DBMDXG010000209 DBMDXG010000000
VERSION     DBMDXG010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5934_length_3597_cov_1.163902"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            345..1163
                     /locus_tag="ACZPD3_06550"
     CDS             345..1163
                     /locus_tag="ACZPD3_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR1415692.1"
                     /translation="MDRQILWDCHMHSSFSADSDTPMEVMIHRAVETGLQGITFTEHL
                     DPDYPVTPDNLDFSLNIPAYKEKLAELSDIYKDKIQVRFGIELGLQMHLGEYFDSLLS
                     QTPFDFVIGSSHLVHGYDPYYPEFFEGRKEFLCYMEYFESILENISAYDGFDVYGHID
                     YVVRYGPNRNREYSYGRYKDILDEILKKLISMGKGIELNTGGYHYGLGEPNPCTAVIR
                     RYRELGGEIITIGADAHTPDKIACAFDKAASVLEACGFRYYTIFKDRKPEFISL"
     gene            1421..3442
                     /locus_tag="ACZPD3_06555"
     CDS             1421..3442
                     /locus_tag="ACZPD3_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylosucrase"
                     /protein_id="HIR1415693.1"
                     /translation="MAKKRLQKKRSSSTVRKQESEILQVTTSKTEPVRVETSKVEPVK
                     VETSKVEPVKLDVPVDLTLYNERFQKHYDELKWLYCELYQDRDDVMTYLHDLTSNMEA
                     FYNSRNSALKASDKKREADPDWYKRNDLVGMMMYVNNFAHTLKGLEEHLDYVEECNVN
                     YLHLMPLLASPKGKSDGGYAVADFRTVQPELGTMEDFSELTSKCHERGINICLDFVMN
                     HTSEEHEWAKRARAGEKEYQDRYFFFDTYDVPALYEKTCPQVFPTTAPGNFTWLDDLH
                     KHVMTTFYPYQWDLNYRNPVVFNEMVYNMLYLANQGVDIVRLDAVPYIWKQLGTNCRN
                     LPQVHTIVRMMRMICEIVCPGVLLLGEVVMAPEKVVPYFGTLEKPECHILYNVTTMAS
                     TWHTVATKDVSLLRRQLDILGSLPKEYIFQNYLRCHDDIGWGLDYDFLKNFSIDEVSH
                     KKFLNDFFTGKYPDTFGRGELYNDDPRLGDARLCGTTASLCGIEKYGFEGNVVGVDRS
                     VRYDITLHAFMLSQSGIPVIYSGDEIGQVNDYSYKDDPDKAVDSRYLHRGEFNWSLAP
                     NRNIADTVQGKLFHALDHLEHIRSSHSVFDTTADLRTIDTWDSSILAIVRENAEEKFI
                     GIYNFSDQDKVAWINEDDGIYTDLISGHEMEAKGVMIPAFGCFWLCKKK"
BASE COUNT         1046 a          739 c          744 g         1068 t
ORIGIN      
        1 cgcgtttttg tcggcgttat agcgctccgc tagcagtttt gcctgccggg ccacattcac
       61 acagtgccgg taccgcttgt gatccagcct ctgttttacc acgcggtcgt attcttccaa
      121 tgtcaaaaca cattcccctt tccaaaaaac agataaaaat taagtaaaaa atgtaaatta
      181 tatgttatac tgtgtataca gtgctttatg aatacactga tccgggcctg taattgtgat
      241 atttcaggtg cagatatatc catttaagag gacacagtat tcatttataa tatatcacac
      301 gcaaatataa cagtactgtt tttacagaag aaaggactga aattatggac agacaaatat
      361 tgtgggattg tcatatgcac tcttcttttt ctgctgactc ggatacgcct atggaagtta
      421 tgatccatcg cgctgttgaa acgggactac agggaattac ttttacggag catctggatc
      481 cggattaccc tgttactccg gacaatctgg atttttctct gaatattccg gcttacaaag
      541 aaaagctggc tgaactttca gatatatata aggataaaat tcaggtacgt ttcggaattg
      601 aactaggact gcagatgcat ctgggagaat attttgactc acttctctct cagacacctt
      661 ttgatttcgt tatcggttca tcccatcttg tccatgggta tgatccttat tacccggaat
      721 tttttgaagg acgtaaggaa tttttatgtt atatggaata ctttgaatcc attcttgaaa
      781 acatttccgc atatgacgga tttgacgttt acggacatat tgattatgtt gtccgttatg
      841 gtcctaacag gaaccgggaa tattcttacg gacggtataa agatattctg gatgagattc
      901 tgaagaaact gatttccatg ggaaaaggca tcgaacttaa taccggcgga tatcattatg
      961 gtcttggaga acccaatcca tgtactgccg taataaggcg ctatagagaa cttggcggag
     1021 agatcatcac catcggagca gatgcgcata ctccggataa aatcgcctgt gcatttgata
     1081 aagctgccag tgtactggaa gcctgtggtt ttcgttacta tacaatcttc aaagaccgta
     1141 aaccggaatt tatttctctg taaaacgtgt cagtacttgc ctatatggca gttcctataa
     1201 tataatatta catatgattt cagaagccca atttccaaac tgctccaaat actgtttctc
     1261 tggtctgcat catagctgat catgataaaa aactgacagc agatatggaa attgcagctt
     1321 tctgtatata ttcgtaactg tccggtgtct ccatatttat gttttatatg tcccgacact
     1381 gcccggttac tgtatttttc ataaaccagg gaggaatatt atggctaaaa aaagactcca
     1441 gaagaaacgt tcttcatcaa cagtcagaaa gcaggaatca gaaattttac aagtgactac
     1501 ttccaaaact gagcctgtca gagttgaaac ttctaaagta gaacctgtta aggtcgagac
     1561 ttccaaagtg gaacctgtta aactggacgt tcctgttgat cttactttat ataatgaacg
     1621 ttttcagaaa cattatgatg agctgaaatg gttatattgc gaattatatc aggatcgtga
     1681 tgatgtcatg acatatctgc atgatcttac ttctaatatg gaagctttct ataacagcag
     1741 aaattcagca ttaaaggcat ctgataaaaa gcgtgaggct gatccggact ggtataaacg
     1801 caatgacctt gtaggcatga tgatgtatgt aaacaatttt gcacatactt taaaaggtct
     1861 ggaggaacat ctggattatg tggaagaatg taatgtcaat tatcttcatc tgatgcctct
     1921 gctcgcatct ccgaaaggaa aaagtgacgg tggttacgca gtagctgact tccgcacagt
     1981 gcagcctgag cttggtacta tggaggactt ttcagagctt acctccaaat gccatgaacg
     2041 cggcatcaac atctgccttg atttcgttat gaaccatact tccgaagagc atgagtgggc
     2101 aaaacgggct cgcgcaggtg aaaaagaata ccaggaccgt tatttcttct ttgataccta
     2161 cgatgttcct gctctctatg agaagacctg tccacaggta ttcccaacta ccgcaccggg
     2221 caactttaca tggctggatg accttcacaa acatgtcatg actactttct atccatacca
     2281 gtgggattta aactacagaa atcctgttgt gtttaacgaa atggtctaca atatgttata
     2341 tctggcaaac cagggtgttg acatcgtacg tctcgatgca gttccttata tctggaagca
     2401 gcttggaaca aactgccgca acctgccaca ggttcacaca atcgtacgta tgatgcgtat
     2461 gatctgcgag atcgtgtgcc cgggagtcct tcttctcgga gaggttgtca tggctcctga
     2521 aaaagttgtt ccatatttcg gtacacttga aaagccggaa tgccatatcc tgtataacgt
     2581 tactactatg gcaagcacct ggcacacagt tgcaaccaaa gatgtatctc tgcttcgcag
     2641 acagctggat attctgggat ctcttccaaa agaatacatc ttccagaatt atctgcgttg
     2701 ccacgatgat attggctggg gactggatta tgatttcctg aagaatttct caatcgatga
     2761 ggtttctcat aagaaattcc tgaatgattt ctttacaggc aaatatccgg atacatttgg
     2821 ccgcggtgaa ctttacaatg atgacccgcg tctgggtgat gcaagacttt gcggtactac
     2881 cgcatctctg tgcggcattg agaaatatgg ttttgaagga aatgtagttg gtgttgacag
     2941 aagtgtgcgc tatgacatca cacttcatgc atttatgctt tcccagtcag gtatccctgt
     3001 aatctacagc ggagatgaga ttggtcaggt aaacgactat tcttataaag atgatcctga
     3061 taaggcagtt gattcccgct atcttcacag aggagaattc aactggtcac ttgctccaaa
     3121 cagaaatatc gcagatacag tacagggaaa acttttccac gcactggatc atctggaaca
     3181 tatcagaagt tcacacagcg tttttgacac aacagctgat cttcgtacaa tcgatacctg
     3241 ggattcttct atccttgcaa tcgtacgcga aaatgcagag gagaaattca tcggaatcta
     3301 taacttcagc gatcaggata aggttgcatg gatcaacgaa gatgacggca tatacactga
     3361 tcttatctca ggacatgaga tggaagctaa aggtgttatg attcctgcat ttggatgctt
     3421 ctggctgtgc aaaaagaaat aaaccattaa tctgcataac gcagaccgca taatgcaaac
     3481 gaggacaatc tgcatatttt acagattgtc ctcgtttttt acttctggac tctttccaaa
     3541 aatcttgcaa aaaatccttt attttcatct tctctgtcca gtgacttctc ataaatc
//
LOCUS       DBMDXG010000210         3578 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5970_length_3578_cov_1.251366, whole genome shotgun sequence.
ACCESSION   DBMDXG010000210 DBMDXG010000000
VERSION     DBMDXG010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5970_length_3578_cov_1.251366"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="ACZPD3_06560"
     CDS             complement(<1..292)
                     /locus_tag="ACZPD3_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HIR1415694.1"
                     /translation="MSELTAKENIKTADTVKRITATAVMAALTTLMTAYIFHIPVGVN
                     GGYVHLGDTMIYLAAAFLPLPYACAAGAIGGGLADLLTAPVWAPATIIIKMLI"
     gene            complement(359..1228)
                     /locus_tag="ACZPD3_06565"
     CDS             complement(359..1228)
                     /locus_tag="ACZPD3_06565"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIR1415695.1"
                     /translation="MKKIAVIHDLSGLGKCSLTAAIPVISVMGVQACPLPTAILSNQT
                     GYGSYFWDDYTDRMELIMDEWKKTGFTPDGVYTGFLGDARQVDLILKFVDMFCTEGTH
                     ILVDPVMGDRGETYKTYSETLCEKMRTLVREATVITPNLTEALLLLYGEEGMKERMKA
                     LSDMEETQLLKEIEKSGRNLTQRFGLEAIVITGVEVSGSDGRARMGNLVLEKEKADWC
                     FSVKEGGSYSGTGDLLASVLSAGMVRGIPMKACVEKAVEFLGGAIHDAVEEGTDRNDG
                     VCFEKYLGILTQI"
     gene            1392..2717
                     /gene="pdxR"
                     /locus_tag="ACZPD3_06570"
     CDS             1392..2717
                     /gene="pdxR"
                     /locus_tag="ACZPD3_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR1415696.1"
                     /translation="MELILDKNKKREHLYLEVFHYYRELIMEKKLPPGSRMPSLRKCS
                     QELKLSRTTIENAYLQLAAEGYIISKAQSGYYVTDIADRQHTSVRKTEKHAQTPCRFD
                     FASSGVDRESFRFDLWRRYIKSALRQDERLLSYGEPQGEADLRDTLADYVRERRNVLC
                     SGEDIVIGAGIQSLLHILCPLLDQRRTVSFPNSSFVQGSTVFHDYGFQVDYRNKDCDI
                     IYVSPAHMTKWGDIMPVSRRLELVNYSAAHGSLVIEDDFENEFVYLQKPTPSLFGLAG
                     GQNVVYLGTFSRLLLPSIRISFMVLPPELSALYRKKADQYNQTASKAEQIALCQFIRD
                     GHLAAQTRKLKRLYSVKLKALRSAVREVFGKDSQIQTGAAGTSLALTLSTTLTWQELQ
                     KKAQTNGLRLQLLREAPGKITLILSCSSMPVADFVPACKLLKNISQIQN"
     gene            complement(2714..3358)
                     /locus_tag="ACZPD3_06575"
     CDS             complement(2714..3358)
                     /locus_tag="ACZPD3_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR1415697.1"
                     /translation="MKVELENVKPMDIEKRSFEIITEELGDKKLVPGTELIVKRCIHT
                     SADFDYAENLCFSENAVEKAIAAIKDGACIVTDTHMAESGINKRVLSRYGGEVFCFIS
                     DEDVAATAKANGTTRATASMDKAAAMGKKLIFAIGNAPTALVRLYELIEDGKLDPELI
                     IGVPVGFVNVVQSKELIMQADVPYIVARGRKGGSNVAACICNALLYMIDNNRGY"
BASE COUNT          896 a          882 c          729 g         1071 t
ORIGIN      
        1 agatcagcat tttgataatt atggttgcag gcgcccatac aggtgctgtc agaagatctg
       61 ccaggccacc tccgatcgct cctgccgcac atgcgtaagg tagtggcaga aatgctgctg
      121 ccagatagat catagtgtca cccagatgta catatcctcc gttcactccc accggaatat
      181 ggaaaatata agctgtcatt aaagtagtca atgctgccat taccgctgtg gctgtgatcc
      241 ttttcactgt atcggctgtt ttgatgtttt cttttgctgt taattcagac ataatatggt
      301 ccctcctgtt atatatttta cttaactatc cggcatttgg tatctatgat ttcctgtttt
      361 atatctgtgt cagtatcccc agatattttt caaagcagac tccgtcattt ctgtcagttc
      421 cttcttccac tgcatcgtga atagctcctc ccagaaattc aacagccttc tccacacatg
      481 ctttcatggg aatccctctc accattcccg cactcagtac agatgccaga agatctcctg
      541 ttccggaata actgccgccc tcttttacag aaaaacacca gtctgctttt tctttctcca
      601 gaaccagatt tcccattcgt gctctgccgt cactgccgga cacctctact cctgtgatca
      661 caattgcttc cagcccaaaa cgctgagtca gatttcttcc agatttttca atttccttaa
      721 gcaactgtgt ttcttccata tcagaaagtg ctttcattct ttctttcatc ccctcttctc
      781 catacagaag aagaagtgcc tctgtcaggt tcggcgtaat cactgtagct tctctgacca
      841 gagtccgcat tttttcacag agagtctctg aataggtttt gtatgtttcc cctctgtctc
      901 ccataaccgg atcaaccagg atatgtgttc cctctgtgca gaacatatct acaaatttca
      961 ggatcagatc cacctgtctg gcatctccca gaaatcctgt ataaacacca tccggtgtaa
     1021 atcccgtttt tttccactcg tccatgatca gctccatccg gtctgtataa tcatcccaga
     1081 aataagatcc atatcctgtc tgattactca atattgcggt gggcagcgga caagcctgga
     1141 ctcccatgac ggaaattaca ggaatcgctg ctgtaagaga gcattttccc agaccggaca
     1201 gatcatgaat tactgcaatt tttttcaatt ttttattcct cttgctttct tttatgcttt
     1261 gtattaatat agcatcacag tggatatatg aatatagcca gatttaatgt ttttttaagg
     1321 gtcagtttat ttttaaccct atagcaattc aagaatgcca tcagcatgtt tgaaggatcg
     1381 gagttttaca tatggaattg attttagata agaataaaaa aagggagcac ctgtatctgg
     1441 aggtgttcca ctattacagg gagctgatca tggagaaaaa gctcccgccc ggaagcagga
     1501 tgccctctct tcgaaaatgt tcgcaggaac taaagctgag ccgcaccacc attgaaaatg
     1561 cctatctgca gctggctgcg gagggatata ttatttccaa agcccagagc ggatactatg
     1621 tgacagatat agctgacaga caacacacat ctgtcagaaa aacagagaaa catgctcaga
     1681 caccttgccg gtttgatttt gcatcttcag gagtagatcg ggagagtttc cgttttgacc
     1741 tttggagacg ttatatcaaa agcgctcttc gccaggatga aagattactg tcttatggag
     1801 agcctcaggg tgaggctgac ctgagagata cacttgccga ttatgtgaga gaacgtagaa
     1861 acgtgctatg tagtggggag gatattgtga tcggtgcagg tatccagagc cttttgcaca
     1921 tcctctgccc gctccttgat caacgtcgga ctgtttcttt tcccaattct tcttttgtac
     1981 agggaagtac tgtgttccat gactatggat ttcaggtaga ttacaggaat aaggactgtg
     2041 atattattta tgtttcccct gcacatatga caaaatgggg tgatattatg cctgtttcca
     2101 gacgcctgga actggtgaat tactctgctg cacatggcag tctggtcatt gaggatgatt
     2161 ttgaaaatga atttgtgtat ctgcagaagc ctaccccttc tctttttggt ctggcaggcg
     2221 gtcagaatgt ggtgtatctg ggaacctttt ccagacttct gctgccctcc atccgtatca
     2281 gttttatggt cctgcctccg gagctgtctg cactgtacag aaaaaaagca gaccaatata
     2341 atcagacagc atcaaaggca gagcagattg ctctgtgcca gtttatccgg gatggtcacc
     2401 tggcagcaca gacccgtaag ctgaaacgtc tgtattctgt aaagctgaaa gcactccgca
     2461 gtgctgtgcg ggaagtattt ggaaaagatt cccagatcca gaccggagcc gcaggtacca
     2521 gcctggccct gactctgtcc acaactctta cctggcagga gcttcaaaaa aaggcacaga
     2581 ccaatggtct gcgcctgcag cttctgagag aggctcccgg taagattacc ctgattctct
     2641 cctgctcctc catgcctgtg gcagatttcg tcccggcatg caagctgtta aagaatatca
     2701 gtcagataca gaattagtat cctctgttat tatcaatcat ataaagcaat gcattacaga
     2761 tacatgcggc aacattgctt ccgccctttc ttcctctggc tacaatataa ggtacgtctg
     2821 cctgcatgat cagttccttg gactgaacca cgtttacaaa gcctaccggc acaccgatga
     2881 tcagctcagg atccagcttg ccatcctcaa tcagttcata gagacgtacc agcgcagtgg
     2941 gggcatttcc gatggcaaag atcagcttct ttcccatggc agcagcctta tccatgctgg
     3001 ctgtagctct tgtggttccg ttggctttgg ctgtggcagc tacgtcctcg tcagagataa
     3061 agcagaatac ttctccgccg taacgggaaa ggactctctt gttgatcccg gactctgcca
     3121 tgtgtgtatc tgtcacaatg caggcaccgt ctttgatagc agcaattgct ttctcaactg
     3181 cattctcaga gaaacagaga ttctctgcat aatcaaagtc tgcgcttgtg tgaatacatc
     3241 tctttacgat cagctctgtt ccgggaacca gctttttatc tccaagttcc tctgtgatga
     3301 tctcaaagct gcgtttctct atatccattg gttttacatt ttccagttct actttcattc
     3361 cttatctccc aaattccata aattcccgta tacaatagtt acatctatca ctcattatag
     3421 caatcctcta aaataatgca tcttaatcca gcccatcaga gtgtgaaatt ctgcgtcaga
     3481 tatctttgcc gtgcatcagc acgcctgcag atatcttcct tgtctttcgc actctgctga
     3541 actgtcttaa atgcattatt tacgaggatt gctatagt
//
LOCUS       DBMDXG010000211         3570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5979_length_3570_cov_1.656673, whole genome shotgun sequence.
ACCESSION   DBMDXG010000211 DBMDXG010000000
VERSION     DBMDXG010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5979_length_3570_cov_1.656673"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="ACZPD3_06580"
     CDS             <1..851
                     /locus_tag="ACZPD3_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HIR1415698.1"
                     /translation="LDIKGLFDNIKHGYLMYMVEKHTQIKWLILYIKRWLTVPFIMSD
                     GSVAERRSGTPQGGVISPVLANLFLHYVFDDFMTKAYPNIWWERYADDGVLHCQSYKQ
                     AAFIKQKLEERFQQFGLELNKEKTRIVYCKDNRRPQNYSCTQFTFLGYTFRPRLNKNK
                     EGKFFVGFTPAVSEKAKTAMKQKIREWKIQLKADLSLKDIGNMINKVVQGWINYYTHY
                     YKSEFYEVLRYINQCLIKWVRRSYKKKNTRSRAEHWLGAVARRDRNLFAHWKFGILPS
                     VGEGAV"
     gene            977..1459
                     /locus_tag="ACZPD3_06585"
     CDS             977..1459
                     /locus_tag="ACZPD3_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019984.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HIR1415699.1"
                     /translation="MGHFGLSLEEEKSRLIEFGRYAKERCSKSGTKPGTFTFLGFTHY
                     CSKSKNGRFRVKRKTSKKKFAKKCREINQLMGHMKTWKLKEITAKLNQILTGYYHYYG
                     ITDNTERITAFRYHVMKSLFYCLNRRSQKKSYNWVEFLNMIDNSYPLVRPRIYVSVYN
                     "
     gene            1816..2376
                     /locus_tag="ACZPD3_06590"
     CDS             1816..2376
                     /locus_tag="ACZPD3_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1415700.1"
                     /translation="MDIYQIIITHNRSWTIREIIAFAVVFLMAVFLAAVLLRLHKIVI
                     IQAVCGLLLLTFLSIVFGSTVFTRTPGIRQYQPEVFWSWKEILGIGTCGRLGSTTGDG
                     LLQENLLNILLLFPAGILLPGVTGRKLKWWMGLLVGIAVSSAIEISQLLLCRGLFEFD
                     DIIHNSLGCMLGSLLGNRMLRLVHGK"
     gene            2766..>3570
                     /locus_tag="ACZPD3_06595"
     CDS             2766..>3570
                     /locus_tag="ACZPD3_06595"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR1415701.1"
                     /translation="MLYIGNHTSSSRGYLAMGKQMLANGGNTFAFFTRNPRGGKAKDI
                     DPQDVQAFGQLAEENHFGKLVAHAPYTMNCCAAKENLRDFAREIMADDLRRLEMTPGN
                     YYNFHPGSHVGQGAEVGISKIAEILNEVLTKDQSTIVLLETMSGKGTEVGRNFEELRQ
                     IIDRVELKDKLGVCLDTCHVWDGGYDIVNDLDSVFEEFDRIIGLDRLKAIHLNDSMNP
                     LGSHKDRHARIGEGEIGLEALVRVINHPATEGIPFILETPNDDEGWTYEI"
BASE COUNT         1158 a          579 c          891 g          942 t
ORIGIN      
        1 aattagatat caaagggctc tttgataaca tcaaacatgg atatctgatg tacatggttg
       61 agaaacatac gcaaataaaa tggctgatac tttatataaa acgttggctt acagtgccgt
      121 tcatcatgag tgatggctcg gttgctgaac gcaggtcagg aacacctcag ggtggagtta
      181 tcagtcctgt gcttgcaaat ctatttcttc actatgtatt tgatgacttt atgacaaaag
      241 catatccgaa catctggtgg gaaagatatg ctgatgatgg agtactgcac tgccaatcgt
      301 acaagcaggc tgcatttata aagcagaaac tggaagaacg ttttcagcag tttggattgg
      361 aactgaataa agaaaagaca cgtattgtct actgcaagga taaccgaagg ccacaaaact
      421 atagttgtac ccaattcact tttctgggat atacgttcag accaaggcta aataagaata
      481 aagaaggaaa gttctttgta ggctttacgc ctgcggtcag tgaaaaagcc aaaactgcta
      541 tgaaacagaa gatcagagaa tggaagatac agttaaaggc ggatctctca ttaaaagata
      601 ttgggaacat gataaacaaa gtagtgcaag gatggattaa ctactacaca cactattata
      661 agtcagaatt ttatgaggtg ttgcgttata tcaatcaatg tctgattaaa tgggtacgac
      721 gaagttataa aaagaaaaac acgagaagca gagccgagca ctggcttggt gcagtagcca
      781 gaagagacag aaacttgttt gcacactgga aatttggaat attaccatct gttggagagg
      841 gagccgtatg agtcgagagg ctcacgtacg gttctgatgg ggactcaagg ggaggttcct
      901 tgggtctacc tactttgtgg tgacattcca atacaaatca gatgcagtat ggttctatga
      961 acacttaaaa catagaatgg gacactttgg attaagcctt gaagaagaaa agagcaggct
     1021 gatagagttc ggacgatacg caaaggaaag atgcagtaaa agtggaacga agccaggtac
     1081 ctttactttc ctaggtttta cacactactg ttccaaaagc aagaatggta gattcagagt
     1141 gaagaggaaa accagtaaga agaagtttgc gaagaaatgc agagaaataa atcaactgat
     1201 gggacacatg aaaacatgga aactgaaaga gattactgct aaactcaatc agatactgac
     1261 tggttattac cattactatg gaatcacaga caatacagaa agaataacag catttcgata
     1321 ccacgtaatg aaaagtttat tctactgtct aaatcggagg agtcaaaaga aaagctacaa
     1381 ctgggtagaa tttctgaata tgatagataa cagctatcca ttggtaagac cccgaatata
     1441 tgtcagtgta tataattgat aaatgtgaac gattcgtttc gcaaagagcc ggatgcggga
     1501 aagccgcaag tccggattct gtgaggggcg tacgaccgca aggggtgcgt ctactcgacc
     1561 catgaaatac ccggggattt atatgaagga tattgataaa agtgctttgt aagaatcagg
     1621 tagttttggt ataattcatt gtaaatgaaa atataaccga atcaggtaag tcctctgcgg
     1681 aggttgcaaa aagcgataag cagtgggtgg gaacttgctt gtatcaggag gattgtaagc
     1741 tccttctgat aaactgcgaa tatccgattg acaggcagtt aaaatcagga ttcatgagat
     1801 ggggagagat agatagtgga catttatcag attattatta cacataatcg aagctggacg
     1861 atcagggaaa ttattgcttt tgcagtggtt ttcctgatgg ccgtttttct ggcagcagta
     1921 ttattaaggc tgcataaaat agtgatcatt caggcagtct gtggactgtt gcttctgacg
     1981 tttctttcaa ttgtatttgg ttctactgtt tttacgagga ctccggggat caggcagtat
     2041 cagccggagg tattctggtc atggaaggaa atcctgggga ttggtacatg tggcagactg
     2101 ggaagtacta cgggagatgg tttactgcag gagaatctgt tgaatattct gctacttttt
     2161 ccggcaggga ttttattgcc gggggtgaca ggcagaaaac tgaaatggtg gatgggattg
     2221 cttgtgggaa ttgcggtatc atctgctatc gagattagtc agctgctgct ttgcagaggg
     2281 ttgtttgagt ttgacgatat tattcataat agtttgggat gtatgctggg gtctttgttg
     2341 gggaatagga tgttaaggtt ggtacatgga aagtaaaggg caggaccaga tgaaaaactg
     2401 acaagtcatc ttgaattgcg atttcaggaa ggtcacgttc aaaacttaac agtttttgtg
     2461 aagcattttg aaaaccgtcc ctgctatcag caggggcggc tttcatttgt gcgcctattt
     2521 attgttttcg ctgacatgaa gatagttcat aagcgcaacc tgaaaaattt tggaacaatt
     2581 gactccgttc acagcaatgc ctttttgacg taaaattgtt tataacagta aaaatgttta
     2641 acaaaatctg ttaaaatgct ataatgcaga tatgaaaaag ataatcttga gaaaatccat
     2701 aatgtatgat aaaaggattg ctataaactg gtactgtgaa aatacgacag aataggagta
     2761 aaaaaatgtt atatatagga aatcacacaa gctcatcccg gggctacctt gcaatgggga
     2821 agcagatgct ggcaaatggc ggtaacacat ttgcattttt taccagaaat ccaagaggag
     2881 ggaaagccaa agacattgat ccgcaggatg tgcaggcatt tgggcagctg gcagaggaga
     2941 atcattttgg aaagctggtg gctcatgcgc cttacactat gaactgctgt gctgccaaag
     3001 aaaacctcag ggattttgcc agggaaatca tggcagatga tctgagacgt ctggaaatga
     3061 caccgggaaa ttattacaat tttcatccgg gcagtcatgt aggacagggt gcagaagtag
     3121 gaatcagtaa aattgcagaa attctcaatg aagtgctgac aaaagatcag agtacgattg
     3181 tacttctgga aactatgtca ggaaagggca cagaagtagg aagaaatttc gaagaacttc
     3241 gtcagatcat tgacagggtg gagttaaaag ataaattagg tgtttgcctg gatacctgtc
     3301 atgtctggga tggtggatat gatattgtaa acgatcttga cagcgtattt gaagaatttg
     3361 accggataat aggtctggac agactaaaag ccatccattt aaatgacagc atgaatcccc
     3421 ttggcagcca taaggaccgc catgccagaa tcggagaagg tgaaatagga ctggaggcac
     3481 ttgtccgcgt gatcaatcat cctgccacag agggaattcc gtttattctg gagacaccaa
     3541 atgacgatga gggatggact tatgaaattg
//
LOCUS       DBMDXG010000212         3560 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_5998_length_3560_cov_1.720439, whole genome shotgun sequence.
ACCESSION   DBMDXG010000212 DBMDXG010000000
VERSION     DBMDXG010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5998_length_3560_cov_1.720439"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            160..1455
                     /locus_tag="ACZPD3_06600"
     CDS             160..1455
                     /locus_tag="ACZPD3_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415702.1"
                     /translation="MLKRFTRYVTQSVAGMIGISVYVLADTFFISVYSGADGLAVLNL
                     ILPVYGLIYAIGAMIGIGSATRYAISRAKGKNTEHYFVQSVTWSILAAVPFMLIGIFI
                     PDKALALLGADAGLIGLGRNYVRIILIATPFFMSNYTFTAFARNDGAPSVAMIGSISG
                     SIFNIIFDYIFMFPVGLGFSGAGLATAICPIVTMSVCTIHYRSSRNHVGFHWKKPSFR
                     HLISCCQLGVSAFVGELSSGVIAIVFNFLILGIAGNMGVAAYGVVANLSIVAFAIFNG
                     LAQGAQPLISESYGKGQPTQVRKLLKWSLLVCLAVEALIQLIIWTSTDMLISIFNSEN
                     NVQLLNYAHTGLRLYFLGFIVAGINIVLVAYFSAVDEPKIAIVGSFLRGIVAIVICAV
                     ILAKLFGLNGIWISLLVAETVTFLTILFLAYKDRRKRTE"
     gene            1534..2352
                     /locus_tag="ACZPD3_06605"
     CDS             1534..2352
                     /locus_tag="ACZPD3_06605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR1415703.1"
                     /translation="MFNEIKDFAAPELDVYARTSEVQLLRYYEPEPGIFIAESPKVIE
                     RALKAGYQPISFLVEHKDLEGEAQEILKQYPDVPVYTAEYELLVKLTGFALTRGMLCS
                     MRRNPLPSVEEICRNASRIAVLENVVNPTNIGAIFRSAAALHMDAVLLTGGCSDPLYR
                     RAARVSMGTVFQIPWTYFDKKTVWPQDGMQILQNLGFKTAAMALRDDSVGIDDKALRS
                     EEKLAVIFGTEGDGLASQTIADCDYTVKIPMSHGVDSLNVAAASAVAFWELGHR"
     gene            2379..2852
                     /locus_tag="ACZPD3_06610"
     CDS             2379..2852
                     /locus_tag="ACZPD3_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR1415704.1"
                     /translation="MSLEKAEKYLEEKGFLDHVIELEASTATVTEAAEALHVEPGMIA
                     KTMSFLQNDKAVLILTEGTARVDNRKYKDTFHIKAKMIPFDEVENIIGHAPGGVCPFG
                     INEGIEVYLDESLKQFTTVYPAAGNDHSAVKLTIPELEQVAEANGWVDVCKETEN"
     gene            3033..3404
                     /locus_tag="ACZPD3_06615"
     CDS             3033..3404
                     /locus_tag="ACZPD3_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415705.1"
                     /translation="MKTVKTPAGIFTINKVKIPSAYTCAAEQKIEYISENHVQIITMN
                     QAVSFGDQILSPRICQSCMNPEKITIYPLEIEYFGEKVFFTDHYSVKEWKKGDPLPEI
                     HEWYPHIKKARCNPCRNCGRC"
BASE COUNT         1103 a          666 c          815 g          976 t
ORIGIN      
        1 cagagtctcc ttgagcagca ttaaaaaatc tgcttaagga gatttttatg tctggagttt
       61 ttgtggttgt atttttgttt tatatggggt ataatggtga aataagtctg cagatgaaag
      121 gaagacaatg ttgacaatat aacgaaggag cgaagtatta tgctgaaaag attcaccaga
      181 tatgtaaccc agagtgttgc cggaatgatc gggatttccg tttatgttct ggcagatact
      241 ttctttatat ctgtatattc cggtgctgat ggactggcag ttctcaattt gattctgccg
      301 gtttatggac tgatctatgc gattggggct atgatcggga ttggttctgc taccaggtat
      361 gccataagca gggcaaaagg aaaaaataca gaacattatt tcgtacagtc agtaacctgg
      421 agcatactgg ctgcagttcc atttatgctg atcggaatct ttattccgga caaagcattg
      481 gctttgctgg gtgcagatgc cggactgatc ggacttggac gaaactatgt gagaattatt
      541 ctgatcgcaa caccattctt catgtcaaat tatacattta ctgcatttgc cagaaacgat
      601 ggagcgcctt ctgttgcaat gatcggttcc atcagtggaa gtatctttaa cattatattt
      661 gattatatat tcatgttccc ggtcggtctg ggattttccg gagccggact ggctactgca
      721 atctgtccga tcgtaacaat gagcgtctgt accatacact acagaagtag cagaaaccat
      781 gtaggattcc attggaaaaa accgtcattc agacacctga tttcctgctg tcagctggga
      841 gtttccgcat ttgtcggtga actgtcttcc ggtgtgattg ctattgtatt taattttctg
      901 atccttggaa tcgcgggaaa tatgggtgtg gcagcctatg gagttgtggc aaatctttct
      961 atcgtggcat ttgcaatttt caacggtctg gcccagggag cacagcccct gatcagtgaa
     1021 agctacggaa aaggtcagcc cactcaggta agaaaacttc tgaaatggag cctgctcgta
     1081 tgtcttgctg tagaagcact gatccaattg atcatctgga cttcaaccga catgctaatc
     1141 agcattttca acagcgaaaa caatgttcag ttattgaatt atgcacatac aggactgcgt
     1201 ctgtatttcc ttggatttat agtggcagga atcaatattg tactggtggc atatttctca
     1261 gcagtagatg aacctaaaat cgcgattgtc ggatcattcc tgagaggaat tgttgctatt
     1321 gttatctgcg cagttatact tgcgaaatta tttggattaa atggaatctg gatctctctt
     1381 cttgtagcgg agacagttac gtttctgacg attctgtttc tggcatataa agacagaaga
     1441 aaaagaaccg aataacactg ttgaaaagga attcttgtat aaacctccaa actgatgtaa
     1501 gataagatta ctcagtttgg aggttttatt tttatgttta acgaaataaa agatttcgct
     1561 gcgccggaac tggatgtata tgccagaaca tctgaagtgc agcttttacg atactatgaa
     1621 ccggaaccgg gaattttcat tgcggagagt ccgaaagtaa ttgagcgagc cctgaaagca
     1681 ggataccagc caatctcttt tctggtggag cataaagatc tggaaggaga ggcacaggaa
     1741 atcctgaaac aatatccgga tgtgccggtc tatactgcgg aatatgaact gttggtcaag
     1801 ctgaccggat ttgctcttac tcgcggaatg ctctgctcaa tgcgcagaaa tccacttcca
     1861 tctgtagagg aaatctgcag aaatgccagc cgcatagcag ttcttgaaaa tgttgtaaat
     1921 cctacaaata tcggagctat tttccgctca gcagcagcac tccatatgga tgcagtactt
     1981 ctgaccggcg gatgcagtga tccgctttac cgacgggcag ccagagtaag catgggaacc
     2041 gtattccaga ttccctggac atattttgat aaaaaaactg tatggccaca ggatggaatg
     2101 cagattctgc aaaatctggg attcaaaaca gctgcaatgg ctctgcgtga cgattctgta
     2161 ggaattgatg acaaagcact tcgttcagaa gaaaaactgg cagtcatttt cggaactgaa
     2221 ggtgacggcc ttgcatcaca gaccatcgca gactgcgatt atacagtaaa gataccaatg
     2281 tcccacggag tagattcatt gaatgtggca gcagcaagtg cagtggcatt ctgggagctg
     2341 ggacacagat aagatataac tcaggaggaa atgaaaaaat gtctttggaa aaagcagaaa
     2401 aatatctgga agaaaaaggc tttcttgacc atgtgatcga actggaagca tccactgcca
     2461 cagtaacaga agcagcagaa gcccttcatg tagagccggg tatgatcgca aagactatgt
     2521 cctttttgca gaacgataaa gcagtcctga tcttaacaga gggtacagcc agagtagata
     2581 accgaaaata taaagatact tttcatataa aggcaaaaat gattccattt gatgaagtag
     2641 aaaacatcat cggtcacgct ccgggagggg tatgcccgtt cggaattaat gagggaattg
     2701 aagtctacct tgacgaaagt ctgaaacaat ttaccacagt atatccggct gccggcaatg
     2761 atcacagtgc agtaaagctc acaatcccgg aactggagca ggtagcagag gcaaacggat
     2821 gggtggatgt gtgtaaagaa acagaaaact gatcgttcat ggtaacaccc atgcattaca
     2881 tttatatgaa agaatcggtt ttaccagact tggcgtgatt ccccagggat tcagaatgcc
     2941 ggatgacatt atggacaaat acaaagaact tgttttttct tcactcatct ttatcttctt
     3001 tgaacatagt gaatataaaa ggtggaaaat taatgaaaac agtaaaaact cctgcgggga
     3061 tatttacgat caacaaagtg aaaattccct cagcatacac atgtgcagca gaacagaaaa
     3121 tagaatatat cagtgaaaat catgtgcaga ttatcactat gaatcaggca gtatcatttg
     3181 gagatcagat attgtccccg agaatctgtc aaagctgtat gaatcctgaa aaaattacaa
     3241 tttatccact ggaaatagaa tactttgggg aaaaagtgtt ttttacggat cactatagtg
     3301 taaaagaatg gaaaaaaggc gatccattac cggaaatcca tgaatggtat ccgcacataa
     3361 aaaaagccag atgcaacccg tgcaggaact gtggaagatg ttaaaattgc acacttgtta
     3421 ctgtttactc cgttcacagt aacgtacact tctttaaatg acagaaacag aatgataggg
     3481 attgttatca acaggaatat aatgtttctg ttaagcaata tttatccaaa agcaataact
     3541 taaatggaga taaaaaatgg
//
LOCUS       DBMDXG010000213         3549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6019_length_3549_cov_1.399942, whole genome shotgun sequence.
ACCESSION   DBMDXG010000213 DBMDXG010000000
VERSION     DBMDXG010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6019_length_3549_cov_1.399942"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(244..1554)
                     /locus_tag="ACZPD3_06620"
     CDS             complement(244..1554)
                     /locus_tag="ACZPD3_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HIR1415706.1"
                     /translation="MDYAKINEAAMGYKDDMTKFLRDLVRIPGESCGEKGHIMRIKEE
                     MEKVGFDKVQIDPMGNILGYMGTGKTLIGFDAHIDTVGIGNIKNWEFDPYEGFESDEE
                     IGGRGTSDQMGGIVSAVYGAKIMKDLGLLNDKYQVLVTGTVQEEDCDGLCWQYIIHED
                     GVRPEFVVSTEPTDGGIYRGQRGRMEIRVDVKGVSCHGSAPERGDNAIYKMADILQDI
                     RALNENDAADTTEIKGLVKMLDEKYNPEWEEARFLGRGTVTTSEIFFTSPSRCAVADS
                     CSVSLDRRMTAGETWESCLDEIRALPAVQKYGDDVTVSMYEYNRPSYTDLVYPIECYF
                     PTWVIPKDHKVTQALEEAYKGLYGEERLGNAETEGMRKARPLTDKWTFSTNGVTIMGR
                     NSIPCIGFGPGAEAQAHAPNEKTWKIDLVRCAAVYAALPTAYCK"
     gene            complement(1700..2929)
                     /gene="dpaL"
                     /locus_tag="ACZPD3_06625"
     CDS             complement(1700..2929)
                     /gene="dpaL"
                     /locus_tag="ACZPD3_06625"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR1415707.1"
                     /translation="MTEGLKWTVNEVPKSDDKHLELMSEENVKKANEFHRSFPQYSVT
                     PLQNLSSLAKYLGVKNIFCKDESYRFGLNAFKVLGGSYAMGRYIAKELGRDISELPYN
                     VLSSDKLREEFGQATFFTATDGNHGRGVAWAANRLGQKAVVRMPKGTTKTRFDNIAKE
                     GATVTIEEVNYDDCVRMAAAEAAKTEHGIIVQDTAWDGYEEIPSWIMQGYGTLVLEAD
                     QQLKEMGVERPTHVFVQAGVGSLAGAVVGYFAHKYKDNPPVMAVCEASAADCLYRSAV
                     AKTGNLVNVTGDLQTIMAGLACGEGNMIGWDILKNHVDVFASCPDWMSAKATRIYANP
                     LGDDPHVVSGESGSVPLGFCFTALHDEDAKDLKEALKLDENSVVLVISTEGDTDPVRY
                     REIVWDGLYGTNESLSK"
     gene            3188..>3549
                     /locus_tag="ACZPD3_06630"
     CDS             3188..>3549
                     /locus_tag="ACZPD3_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR1415708.1"
                     /translation="MAVFHAFRALRPTPEKAADVAALPYDVVNREEAKSIGDENPLSF
                     LHIDRPEMDLEPETDLYDDRVYEKAKENLDNMEEKGILVQDQKACYYIYELVRKGKTQ
                     TGIVGCSSIDDYMNGVVK"
BASE COUNT          974 a          783 c          710 g         1082 t
ORIGIN      
        1 tgtttaattt atcaatgtaa tcctgtaatg ttttcataaa atcaatacct gctatgcttt
       61 gatagcttat cctttcctga tatgaaataa ttgaatctct gcgtttggca gaacaccggt
      121 tatccggctg tatctgttta tcttgtgtcc ctgcctgtta cagcagggat tctgtccgga
      181 cacatattaa gcctttagga tgataggaaa tccgtcgcct aaaaaaattt gaaaatttat
      241 ttattatttg cagtatgcag ttggaagagc agcgtataca gcagcgcatc ttacaaggtc
      301 gatcttccat gttttttcgt tcggagcatg agcctgtgct tctgcaccag gtccgaatcc
      361 gatacaagga atgctgttac gtcccatgat tgttacacca ttagtagaga atgtccattt
      421 gtctgtaagc ggacgagctt ttctcatgcc ttctgtctca gcatttccga gacgctcttc
      481 gccgtacagt cctttatatg cttcttccag agcctgtgtt actttgtgat ctttcgggat
      541 tacccatgtc gggaagtagc actcgatcgg atatacaagg tctgtatagg acggacggtt
      601 gtattcatac atggatactg ttacgtcgtc gccatatttc tgtacagccg gaagagcacg
      661 gatctcatcc aggcagcttt cccatgtttc gcctgctgtc atacgacggt caagagatac
      721 agagcaggag tcagctactg cacagcggct tggggatgtg aagaagattt cggaagtagt
      781 aacagtgcca cgtccaagga aacgtgcttc ttcccattca gggttgtatt tctcatcaag
      841 catttttaca agacctttga tctctgtggt atcagcagca tcgttttcgt ttaatgcgcg
      901 gatatcctgg agaatatcag ccattttgta gattgcattg tcaccacgtt ccggagcgga
      961 accatggcag gaaacaccct ttacatctac gcggatttcc atacgtccac gctgtcctct
     1021 gtagataccg ccgtctgttg gttctgtgga aacaacgaat tcaggacgaa caccatcttc
     1081 atggatgatg tactgccagc agagaccgtc acagtcttct tcctgtacag tacctgtcac
     1141 aagtacctga tatttatcgt ttaaaagacc aaggtctttc atgatcttag caccgtatac
     1201 tgcagaaaca ataccgccca tctggtcaga tgttccacgt ccgccaattt cttcatcaga
     1261 ttcgaaacct tcgtatggat caaattccca gttcttgata ttaccgatac cgactgtatc
     1321 aatatgagcg tcaaaaccaa tgagagtttt acctgttccc atgtagccga ggatattgcc
     1381 catcggatcg atctgaactt tgtcgaatcc gactttttcc atttcctctt tgattctcat
     1441 gatatgtcct ttttctccgc agctttcgcc aggaatgcga accagatcgc gaaggaactt
     1501 agtcatatca tccttataac ccattgcagc ttcgttaatt tttgcgtaat ccattttttt
     1561 accctcccaa tatgtataaa atattatgat gttagggtca aaaggtattt tgcccctaat
     1621 ctgatatcat attattagtt actggtatat cattttcgag ataaccatac cacttacctg
     1681 tctgtgttca ccaaaaacat catttgctta aagattcgtt tgttccgtat aatccgtccc
     1741 atacgatctc gcggtagcga acaggatcag tgtcaccttc tgtggaaata acaagtacta
     1801 cggaattctc atccagtttc agcgcttctt ttaagtcttt tgcatcttca tcgtgaagag
     1861 cagtgaagca gaaaccaagc ggaacggaac cggattcacc agagacaacg tgtggatcat
     1921 ctcccagtgg attagcgtag atacgtgttg cctttgcgct catccagtca ggacaggatg
     1981 caaatacatc tacgtgattt ttcaggatat cccatccgat catatttccc tcaccgcatg
     2041 caagaccggc cataattgtc tgaagatcac ctgtaacatt taccagattt cctgtctttg
     2101 caacagcgga gcggtacaga cagtcagcag cacttgcttc acagacagcc ataaccggcg
     2161 ggttatcttt atatttatga gcgaaataac cgactacggc acctgctaaa gaaccaacac
     2221 cggcctgaac aaatacatgt gtcggacgtt caacacccat ttcttttaac tgctggtcag
     2281 cctcaagaac cagtgttccg tatccctgca tgatccagga tggaatttct tcatatccgt
     2341 cccatgcggt atcctgaacg atgataccat gttctgtttt ggcagcttct gctgcagcca
     2401 ttcttacgca gtcatcatag tttacttctt cgatcgtaac agtagcacct tctttggcaa
     2461 tgttatcgaa acgagtcttt gttgttccct taggcattct tacaactgct ttctgtccaa
     2521 ggcggttagc tgcccatgca actccacgtc catgattacc gtctgttgca gtaaagaatg
     2581 tagcctgtcc gaattcttct cttaatttat cagaagagag aacattgtat ggcagctcgc
     2641 tgatatcacg tccaagttct tttgcgatat agcggcccat tgcataagaa cctccaagta
     2701 ctttgaaagc atttaagcca aagcgatagg attcatcctt acagaaaata ttttttactc
     2761 ctaaatattt cgcaagggat gaaaggttct gaagaggcgt cacactgtac tgtgggaaac
     2821 ttctgtggaa ctcatttgct ttctttacat tttcttcgga catcagttcc aggtgtttat
     2881 catctgattt cggcacctcg tttacagtcc attttaatcc ttctgtcata aaatgacctc
     2941 ccttaaaaat tatcatttgg tttccttaac ttgtgattct atcataccat atattttaaa
     3001 aattgcaata gaaaaataca aaaattttta ggtttttcaa aaaaaaatta ggtttcataa
     3061 tttgtacatt gatataagga tttggatatg ataaaataat gagtaatgtt cgttttattt
     3121 ttacaggata gacagtaaag tgggtataaa agagcaatac aaaagataca gaagaggaga
     3181 aaatattatg gcagtatttc atgcattccg ggcacttcga cctacaccgg aaaaggctgc
     3241 tgatgtggca gcacttccat atgatgtggt aaaccgggaa gaagcaaaat caatcggaga
     3301 tgagaatccc ctgtcctttc ttcatattga cagaccggaa atggatctgg aacctgagac
     3361 agatctgtat gatgatcggg tttatgaaaa agcaaaagaa aatcttgaca atatggaaga
     3421 aaagggaatt cttgttcagg atcagaaggc ctgttactat atatatgaac ttgtaagaaa
     3481 aggaaagaca cagaccggta tcgtaggatg cagctccatt gatgattata tgaatggtgt
     3541 agtgaagaa
//
LOCUS       DBMDXG010000214         3524 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6062_length_3524_cov_1.681274, whole genome shotgun sequence.
ACCESSION   DBMDXG010000214 DBMDXG010000000
VERSION     DBMDXG010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6062_length_3524_cov_1.681274"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(141..524)
                     /locus_tag="ACZPD3_06635"
     CDS             complement(141..524)
                     /locus_tag="ACZPD3_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415709.1"
                     /translation="MKITEELLNEMKIKDENFSDGLIKPDGDYVRIPRGHLHGMMELL
                     PWTENEIWKMIPDDDSPLFWLIEKTGCVLTDYNNSIGMKMTPAQQTVFDMMRKHGVLT
                     DDYYDLTKQREKVREAREQKENRKQ"
     gene            complement(1017..2015)
                     /locus_tag="ACZPD3_06640"
     CDS             complement(1017..2015)
                     /locus_tag="ACZPD3_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415710.1"
                     /translation="MKRRFGGTVIAMVLCFILTVVSVYADDGKGQNPDYVTDYYMIVQ
                     STQGGVDIYDEADTQSVKLNDSKISNGTAIHVLGEKNGADNKKWAYTQYHGMNGYVPM
                     DDLDPASREEAANEEYRTFGGKDVDFEVKVHGNVSVYNGPGEKFDQVSGTEGIADGTT
                     VHIFQHVQGEDGTNWGKTDTDGVTQGWLNLDRDTDYVNENVSADAPEATGTSGNVPVA
                     AVTPTPEATPTPKATPTPEVTPTEKVTLTPEATPTEEATPTPEATPTEEATPTPEETE
                     KPASTEDKESDDKQSQKTSGKNVKADSGMKNPVIWISGIGIIVIIILLIYFLKKKK"
     gene            complement(2035..>3524)
                     /locus_tag="ACZPD3_06645"
     CDS             complement(2035..>3524)
                     /locus_tag="ACZPD3_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012340.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR1415711.1"
                     /translation="SKAYNSAAYLKNASVSQYSEERMKEMLLNDHAVSIMLYMKESYV
                     NPDTAAYCYPVGKSNSTVINHIVTVVGWDDTYSKDNFLPVSNVTSDGAWIIKNSWGEK
                     KGDGGYYYLSYQDPNISKLVSAEAVAASDQKYRNNYFYDGSSALSVIPIQAGQSVAAV
                     YETTAGKGKAEVLGEVNLVTNSDNACYKIQIYTDLTDPYDPESGTAAYAAPYEFEQPI
                     AGVQTISVPEVVLKQGSRYSVVITNSGIEKISFGVEAKSSYGNWFTCTAGIETGQTFY
                     KGASETARWTDGKTKNWTARIKAHTRPLNQSWVPDTPVFQVKAYNSGYNLISWEKVSG
                     ATGYYVYRKPAAGGKWSQIADVGTSELKYKDSKVTANASYRYTVKAYYEASGKRYSGK
                     YKTGDVIKAAPAVQKVTSVKSEKNGIRIRWKPQKKCDGYYIYRKKKGGSYQLIKKISN
                     GNSSSYLDKKAQKGVSYYYAVKAYVKEPYGNTYSKYKSSSAVKRK"
BASE COUNT          848 a          851 c          581 g         1244 t
ORIGIN      
        1 gttaaaagca gcatatcagg ttatgatcca taactctgat atgctgcttt ttaatgtcgt
       61 atattgtttt ttctgcatcg gatgccttta atgagcatgc ttttccatcg ctgcactccc
      121 atatattcat atacacttaa ctattgtttc ctgttctctt tttgttctct ggcttctctt
      181 actttctccc tctgtttcgt cagatcgtaa taatcatctg taagaacccc atgttttcgc
      241 atcatatcaa acacagtctg ctgagcaggt gtcatcttca tgccaataga attattataa
      301 tccgtaagaa cgcatcctgt tttctcaatc agccagaaca gcggagaatc atcatccggg
      361 atcattttcc agatttcgtt ctctgtccat ggaagcaatt ccatcattcc atgaagatgt
      421 cctctgggta ttcttacgta atcgccgtcc ggcttgatca gaccgtcaga aaagttctca
      481 tccttaattt tcatctcatt taacagttct tctgttatct tcatcctctt ttcctcaccc
      541 tcgagtattg ctttttccta ttatacgttt cccgccagag ttacgcaagt aaaaagtctg
      601 aatagcctct ttcctcaaaa atatatgtta ctgttcactc cgttctcagt aacatagcca
      661 aaattcattc cagattgcct gcggcaatgg aattttggct tgtatgtctc gggattttgg
      721 catattcatg ccaaaacacc tcgcgggata gtggtgtgtg aacagtaaca aatatatcac
      781 gttctataaa attccatctt gattaaattg caaaaaaatt gtagactata tatgcacaca
      841 cattaatagt ttttgttaat taagactgat ttgaggtttc ctcataacgt ctcaattata
      901 cacacacatt ttctaaaaag agcagctgca tcatccgatg cgctgctctt ttagttccac
      961 tgtttatttt gtaactgttc tctgccgtcg acggcattct ttaatgattt taactcttac
     1021 tttttcttct tcaggaaata aataagtaaa attatgataa caataatccc gataccacta
     1081 atccagatta caggattctt cattcctgaa tctgctttta cattctttcc tgatgttttc
     1141 tgactctgct tatcatcgga ttccttgtct tctgtcgaag caggcttctc tgtttcttcc
     1201 ggtgttggcg ttgcttcttc tgtaggtgtt gcctccggtg tcggtgttgc ttcttctgta
     1261 ggtgttgcct ccggtgtcag tgtcactttt tctgtaggtg ttacctctgg tgttggtgtg
     1321 gctttcggag ttggagttgc ttccggtgtt ggtgtaacag cagccaccgg aacatttcct
     1381 gatgtgcctg tagcctccgg tgcatctgcg gatacatttt catttacgta atctgtatca
     1441 cgatcaagat tcagccatcc ctgtgttaca ccatctgtgt ctgttttccc ccagtttgtt
     1501 ccgtcttctc cctgaacatg ctgaaaaata tgtacagttg tcccatctgc gattccctct
     1561 gttcctgaaa cctgatcaaa tttctctcca ggtccgttat aaaccgatac gtttccatga
     1621 acctttactt cgaaatccac atctttgcct ccgaaagtac gatattcctc attggcagct
     1681 tcttctctgg aagccggatc cagatcatcc ataggaacat atccgttcat tccatggtac
     1741 tgtgtatatg cccatttttt attgtctgct ccattcttct cacccagaac gtgaatggca
     1801 gttccatttg agatcttgct gtcattcagt ttaacactct gagtgtctgc ttcatcataa
     1861 atatctaccc cgccctgtgt actctgtacg atcatataat aatctgttac ataatccgga
     1921 ttctgaccct ttccatcatc cgcataaaca gaaaccactg tcagaataaa gcacagtacc
     1981 attgctatta ctgttcctcc aaaccttctc ttcataatct ctttccttca tttcttattt
     2041 tctttttaca gcagatgaac ttttatattt gctgtatgta tttccatatg gctctttaac
     2101 atatgctttt accgcataat agtatgatac acctttttgt gcttttttgt ccagataaga
     2161 agaactattt ccattggaaa ttttctttat caattgatag gaaccacctt ttttctttct
     2221 gtaaatataa tagccgtcac attttttctg aggcttccac cggatacgga ttccattttt
     2281 ctcagatttc acagaagtaa ccttctgcac tgcaggtgct gctttaatca catcgccggt
     2341 tttatattta cctgaatatc tttttccgga tgcttcataa tatgctttta ctgtataacg
     2401 gtatgatgca ttagccgtaa ccttactatc tttatatttt aattccgatg tcccgacatc
     2461 tgcaatttgg gaccacttcc cacctgctgc aggttttctg tacacataat atcctgtagc
     2521 tccggaaact ttttcccatg aaataaggtt atatccggaa ttatatgcct tcacctgaaa
     2581 taccggtgta tccggcaccc atgactgatt caggggcctt gtatgtgcct tgatccttgc
     2641 cgtccagttt ttcgtctttc catctgtcca cctggccgtt tccgatgcac ccttatagaa
     2701 tgtctgccct gtttctatcc ccgccgtaca ggtaaaccag ttcccataac tggactttgc
     2761 ttccactcca aagctgattt tttcaatccc actgtttgtg atcacaacag aatatctgct
     2821 tccctgtttc aaaaccacct ctggtacact gatagtctgt acacctgcga ttggctgttc
     2881 aaactcatat ggagcagcat atgcagcggt cccgctttcc gggtcatatg gatctgtcag
     2941 atcggtgtat atctgaattt tgtaacaggc attgtcactg tttgtaacaa gattaacctc
     3001 ccccaggact tctgcttttc cctttcctgc agttgtctca taaactgctg ctactgactg
     3061 tcccgcctgt atcggaatca ctgaaagtgc tgaagaacca tcataaaaat aattattcct
     3121 gtacttttga tccgaagcag ccactgcctc tgcacttaca agtttactga tattgggatc
     3181 ctgataagaa agatagtaat atcctccgtc tcctttcttc tccccccaac tgtttttgat
     3241 gatccacgca ccatcagatg ttacattgga aacaggaagg aaattatcct ttgaatacgt
     3301 atcatcccat ccgaccactg tcacaatatg attaattaca gtcgaatttg attttccaac
     3361 aggataacaa tatgcagctg tgtccggatt tacataactt tccttcatat aaagcataat
     3421 ggatactgca tggtcattca gcagcatttc tttcatacgt tcctcactgt attgggaaac
     3481 agatgcattt ttcagatatg ctgcagaatt ataagcctta cttt
//
LOCUS       DBMDXG010000215         3507 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6083_length_3507_cov_1.652378, whole genome shotgun sequence.
ACCESSION   DBMDXG010000215 DBMDXG010000000
VERSION     DBMDXG010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6083_length_3507_cov_1.652378"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="ACZPD3_06650"
     CDS             complement(<1..338)
                     /locus_tag="ACZPD3_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR1415712.1"
                     /translation="MAKRIITISREFGSGGRFIGEEVAKKLGIAYYDKNIIGQIAEKS
                     GLSPEYIQENAELSPKKGLFAYAFSGRDITGKSVEDMVYEVQRNIILELAEKEPCVII
                     GRNADYILKDR"
     gene            complement(351..1718)
                     /locus_tag="ACZPD3_06655"
     CDS             complement(351..1718)
                     /locus_tag="ACZPD3_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415713.1"
                     /translation="MAESNKMKDMPVNKLMIQMGIPMILSMALQAVYNIVDSAFVGNM
                     RVGSEAALNALTLVFPVQMLMVAAGIGTGVGTNALLARTLGQGNREKAAKVAGNSLFL
                     GVIIYVVCLLFGIFGVKVYISSQTVDAEVLEMGVSYLRICCVISFGIIFFSLFEKLLQ
                     ATGRSLYSTIGQVVGAVVNIILDPIMIYGIGPCPEMGVKGAAYATVIGQVASAVLLLI
                     FHMKLNREFGHGPKYMKPNAGVIKEIYAIGLPAIIAQALMSIMVYVMNLILKFNPSAQ
                     TAYGLFYKVQQFVLFLAFGLRDAITPIIAFAYGMRSKKRIQDGIKYGLIYTIALMILG
                     IAITEIFPGAFAMLFNAGQSREYFIAAMRVISVSFLFAGINVAYQGIYQALDGGLESL
                     VISLLRQLIIILPLAGIFSLFVRNGQMGVSLIWWAFPITEIISCFVGYVFLKKIRKNR
                     VDVLN"
     assembly_gap    2020..2029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2030..2306)
                     /locus_tag="ACZPD3_06660"
     CDS             complement(<2030..2306)
                     /locus_tag="ACZPD3_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014602.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR1415714.1"
                     /translation="MKYEIGDFVSKPVTGICKIENILYLNPQDEKNDKLYYLMKPVED
                     EKEKIYVPVSSSDSRLRLCLTKEEAWNLIKRIPDIPTAWTNNEKMREQ"
     gene            complement(2335..2556)
                     /locus_tag="ACZPD3_06665"
     CDS             complement(2335..2556)
                     /locus_tag="ACZPD3_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415715.1"
                     /translation="MYEYEIFIEDINPCGGEQYSKKTLIEAEAASPEAYVKENGRFPI
                     LESTRNESGDVVIVTGDNQGSFVRYTFTE"
     gene            complement(2830..>3507)
                     /locus_tag="ACZPD3_06670"
     CDS             complement(2830..>3507)
                     /locus_tag="ACZPD3_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family accessory protein
                     TnpB-related protein"
                     /protein_id="HIR1415716.1"
                     /translation="INTDAVCTIMRADGTVLGRKFIDHPSEKDRMYRALGRIRRFQRE
                     HGSAQTQGRWTYTKRLNTELGKKIAGAIVRYAEENHADVIVFEYLEMQGKIPGKKKQK
                     LHLWRKRDIQRRCEHQAHRKRMRVSRICAWNTSRLAYDGSGAVTRDWEDYSLCTFQTG
                     KRYNCDLSASYNIGARYFIRELLKPLPVTERSLLEAKVPPVKRRTSCVYADLRKLHSE
                     MELLKAA"
BASE COUNT          955 a          856 c          571 g         1115 t
ORIGIN      
        1 cggtctttca gtatatagtc tgcatttctt ccaatgatca cacaaggctc cttctcagcc
       61 aattctaaaa taatatttct ctgcacctca tacaccatat cttccacaga ttttcctgtg
      121 atatcacgac cggaaaatgc atatgcgaac aaaccttttt tcggagataa ttccgcattt
      181 tcctgaatat attccggtga taagccggac ttttctgcaa tctgaccaat gatattttta
      241 tcataatagg caattccgag tttctttgcc acttcttctc cgataaaacg tccaccgctt
      301 ccaaattccc tgctgatcgt aataattctt tttgccatct ctgattcctc ctagtttaaa
      361 acatcaaccc tattttttct gattttcttt aaaaatacat atcccacaaa acatgaaatt
      421 atttcagtaa ttggaaatgc ccaccagatc aacgaaactc ccatctgacc atttctgaca
      481 aacagagaaa agattcccgc cagtggcagg ataataatca gctgtctcaa aagtgaaatc
      541 accagtgatt ccaggccacc atccaatgcc tggtaaattc cctgataagc aacattaatt
      601 ccggcaaaca agaaactcac tgaaatcact ctcatcgcgg caataaaata ttctcttgac
      661 tgtcctgcat taaacagcat tgcaaacgct ccggggaaaa tctccgtaat tgcaattccc
      721 agaatcatta atgcaatggt ataaataagt ccatatttga taccatcctg aattctcttt
      781 ttacttctca ttccataagc aaatgcaatg atcggagtga tcgcatctcg cagaccaaaa
      841 gcaaggaaga gaacaaactg ctggacttta tagaacaatc cataggctgt ctgtgctgat
      901 ggattgaact tcaggatcag attcatcaca taaaccataa tagacatcag agcctgcgca
      961 ataattgccg gaagtccgat tgcatagatt tcttttataa caccagcatt cggtttcatg
     1021 tatttcggtc catgcccgaa ttccctgttc agtttcatat gaaaaataag cagtaataca
     1081 gcagaagcaa cctgaccaat tacagttgcg taagctgcac ctttcactcc catttcaggg
     1141 caaggtccaa taccgtagat catgatcgga tcgagaatga tatttacaac ggcaccaacc
     1201 acctgtccga ttgtagaata aagagaacga cctgttgcct gtaatagctt ttcaaataaa
     1261 gaaaagaaaa taataccaaa agaaatcaca cagcatatcc gcagatagct gactcccatt
     1321 tcaagtactt ctgcatcaac tgtctgtgat gaaatatata ctttcactcc aaaaatacca
     1381 aacagtaagc agaccacata aatgatcaca cccaggaaca gactgtttcc tgctaccttt
     1441 gcagcctttt ccctgtttcc ctgtccaagt gtccttgcaa gaagtgcatt ggttcctaca
     1501 cctgttccga ttcctgccgc taccataagc atctgaaccg gaaataccag agtgagggca
     1561 tttaatgcag cttcactgcc tactctcata tttcctacaa acgcactgtc tacaatgtta
     1621 tagaccgcct gcaatgccat ggataagatc atagggattc ccatctggat catcagctta
     1681 ttcaccggca tatccttcat tttgttgctt tctgccattt tactttaact cctctctttt
     1741 ctgcacaaaa aaagtgcgca ccctgtcaca taagctggac ttacgctgac aggctacaca
     1801 ctttttcatg gcaaatattc tattttaaca aaagaaaaac gagtagcatg aaactggatt
     1861 tacgctcatc tgctactcgt tcgttatcat tatatttcct gttttctaaa aagtcaaacg
     1921 aaatattttt tacaatttac attttacaat gcacatcaat ctattttatt ttgatcgatg
     1981 taatcaataa tcgttttaca gatttcctgt tttggctttn nnnnnnnnnt ttgttccctc
     2041 attttttcat tgtttgtcca tgctgtcggg atatccggaa ttcgtttgat cagattccag
     2101 gcttcttctt ttgtcagaca taatctcagt cttgaatctg agcttgaaac aggaacatat
     2161 attttttcct tttcatcttc tactggtttc atcagataat ataacttatc gtttttttca
     2221 tcctgcggat tcaaatataa aatattttct attttacaga tacctgtaac tggtttgctg
     2281 acaaagtctc caatttcata tttcacaaaa tccactcctt gctgaccgac attcttattc
     2341 cgtaaaagta taacgtacaa aagaaccctg attgtctcct gttactatta caacatctcc
     2401 actctcgttg cgtgtggatt ccagaatcgg aaatcttcca ttttctttta cataagcttc
     2461 cgggctggct gcttctgcct cgatcaaagt ttttttactg tactgttctc ctccacacgg
     2521 atttatatcc tcaataaaga tttcatactc atacatatgc agttcctcct gagattcatt
     2581 ttataaaaac aagcagcata actgttgttt tccgactcat tatgctactc gcccttattt
     2641 ttattataaa gccattttac tttgatgtga acctcacatg actgaagtca cgtgcttcca
     2701 gacgctttag gcgtcagact gaatatcggc ggatacgcct gtaacttagg tgcgcagtta
     2761 tgcgcatttc ccatgccaga tatggctggt tcccacatta caggtagtcc gctgtatatc
     2821 tgcctgtatc tatgctgctt ttaagagttc catttctgaa tggagttttc tcagatctgc
     2881 atagacgcat gaggttctac gctttacagg agggactttt gcctccagca aagacctttc
     2941 cgttaccgga aggggtttta aaagttctct tataaaatat ctggctccta tattatagga
     3001 tgctgacagg tcacaattat atcgttttcc tgtctggaaa gtacagaggc tgtaatcttc
     3061 ccagtcacgt gttaccgccc cggaaccatc ataagccagt ctgctggtgt tccatgcaca
     3121 gatccgggat acccgcatcc ttttcctgtg tgcctgatgt tcacagcgtc tttggatatc
     3181 ccgctttctc cacaggtgca gtttctgctt tttctttccc ggtatcttcc cctgcatctc
     3241 tagatactcg aacacgatca catctgcatg gttttcttcc gcatatctta cgatcgcacc
     3301 tgcaatcttt ttacccagtt ctgtgttcag acgtttcgta tacgtccatc ttccctgtgt
     3361 ctgcgcagaa ccatgttccc tctggaatct gcggatccgt cccagcgcgc ggtacatccg
     3421 gtctttttca ctgggatgat ctatgaattt tcttcccagg acagttccgt ctgcccgcat
     3481 gatggtacag actgcatcgg tattgat
//
LOCUS       DBMDXG010000216         3502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6095_length_3502_cov_2.049397, whole genome shotgun sequence.
ACCESSION   DBMDXG010000216 DBMDXG010000000
VERSION     DBMDXG010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6095_length_3502_cov_2.049397"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="ACZPD3_06675"
     CDS             <1..1548
                     /locus_tag="ACZPD3_06675"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR1415717.1"
                     /translation="LKESDKKDGKLILVTAINPTPAGEGKTTTTVGLADGMQRLGKSV
                     MVALREPSLGPVFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMIDNH
                     IFQGNALNIDPRKITWRRCVDMNDRQLRNVVDGLGGKTNGMPREDGYDITVASEIMAV
                     LCLASDIKDLKERLSKIIIGYTYGKVSEQKPVTAGDLHAEGAMTALLKDALKPNLVQT
                     LEHVPAIVHGGPFANIAHGCNSVTATKMAMKLADYAITEAGFGADLGAEKFLDIKCRM
                     AGLKPSAVVIVATVRALKYNGGVAKADLNNENLEALEKGIPNLLKHVSNIKNVYKLPC
                     VVAINAFPTDTKAELDFVEAKCKELGVNVALSEVWAKGGEGGIKLAEEVIRLVEEPND
                     FSYAYELEGSIEDKLNQIVQKVYGGKKVVLTANAQKQAKQLEALGFGNCPICVAKTQY
                     SLTDDQTKLGAPTDFEVTVRNLKISAGAGFIVALTGEIMTMPGLPKVPAAERIDVDET
                     GKITGLF"
     gene            complement(1657..3099)
                     /locus_tag="ACZPD3_06680"
     CDS             complement(1657..3099)
                     /locus_tag="ACZPD3_06680"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR1415718.1"
                     /translation="MGGRITMKKTDYRVERDSIGVKDIPEDVYYGVQSLRAAENFHIT
                     GLNMHPEIINSLAYIKKAAAITNCEVGLLEKKKAQAIVQACDEIVSGKFHNEFIVDPV
                     QGGAGTSLNMNANEVIANRAIEILGGKKGDYTIINPNDDVNCGQSTNDVIPTAGKMTS
                     LRLLQNLKKQLLRLYDALNEKATEFDHIIKMGRTQMQDAVPIRLGQEFKAYSVAIMRD
                     IHRMDKAMDEMRTLNMGGTAIGTGINADEGYLRRIVPNLTEISGMDFIQAFDLIDSTQ
                     NLDPFVAVSGAVKACAVTLSKMSNDLRLMSSGPRTGFGEINLPAKQNGSSIMPGKVNP
                     VIPEVVNQVAFNIIGNDVTITMAAEAGQLELNAFEPIIFYCMFQSIDTLGYAVQTLVD
                     NCIVGITANEERCRYLVENSVGIITAISPHLGYQKAADIAKKAIKTGESVRSLILKEK
                     LMDEDELNRILDPIHMTEPGISGKDYLIKK"
     gene            3197..>3502
                     /locus_tag="ACZPD3_06685"
     CDS             3197..>3502
                     /locus_tag="ACZPD3_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1415719.1"
                     /translation="MFQGMEYIYEVYKEKSFSKAAAALFISQPSLSANVKRVENRIGY
                     PIFDRSTKPLQLTEVGKHYIQAVEKVMDIENNLENYLLDLGNLKTGTLNVGGSNFFSS
                     "
BASE COUNT         1056 a          755 c          691 g         1000 t
ORIGIN      
        1 ctgaaagaaa gtgataagaa ggatggaaaa ttaatcctcg ttacagctat caacccaact
       61 ccagcaggag aaggtaaaac tactactact gtaggtcttg cagatggtat gcagagactt
      121 ggcaagagtg ttatggtagc tcttcgtgag ccatccttag gaccggtatt cggtgtaaaa
      181 ggtggtgcag caggcggcgg atatgcacag gttgttccta tggaagatat taaccttcac
      241 ttcacaggtg acttccatgc cattggcgca gctaacaact tactggcagc tatgatcgat
      301 aaccatatct tccagggcaa tgcattaaac atcgacccac gtaagatcac atggagaaga
      361 tgtgtagata tgaacgaccg tcagcttcgt aacgtagtag atggtcttgg cggaaagaca
      421 aacggtatgc ctcgtgaaga cggatacgac atcacagttg catctgaaat catggcagtt
      481 ctctgtcttg caagcgacat caaagacctg aaagaaagat tatccaagat catcatcgga
      541 tatacatacg gaaaagtttc cgagcagaaa cctgtaacag caggtgacct tcacgcagaa
      601 ggcgctatga cagccctgtt aaaagatgct ctgaaaccaa accttgtaca gactctggaa
      661 cacgttcctg caatcgtaca tggcggacca ttcgcaaaca tcgctcatgg atgtaactct
      721 gtaacagcta caaagatggc tatgaagctt gctgattatg caatcacaga agctggtttc
      781 ggtgctgacc ttggtgctga gaaattcctt gatatcaaat gccgtatggc tggtttaaaa
      841 ccaagcgcag ttgtaatcgt agctacagta cgtgccctga aatacaacgg cggcgttgct
      901 aaagctgacc ttaacaatga aaacctggaa gctcttgaaa aaggtatccc gaacctgtta
      961 aaacacgttt ccaacatcaa aaacgtatac aaacttccat gtgttgttgc tatcaacgca
     1021 ttccctacag atacaaaagc agagcttgac tttgtagaag ctaaatgtaa agaactgggc
     1081 gttaacgttg ctctttctga agtatgggca aaaggcggcg aaggcggaat caaacttgca
     1141 gaagaagtta tccgtcttgt tgaagaacca aacgacttct cctatgcata tgaactggaa
     1201 ggttctatcg aagacaaact gaaccagatc gttcagaaag tatatggcgg taagaaagta
     1261 gttctgacag ctaacgctca gaaacaggcg aaacagttag aagctcttgg ctttggcaac
     1321 tgcccgatct gtgtagctaa gacacagtac agcttaacag atgaccagac taaactgggt
     1381 gctcctactg acttcgaagt tacagtacgt aacctgaaga tttccgctgg tgcaggattc
     1441 atcgtagctc ttacaggcga gatcatgaca atgccaggtc ttcctaaagt tccggctgct
     1501 gagagaatcg acgtagacga gacaggtaag atcacaggtc tgttctaatc cggttgaaat
     1561 caatcgaata attcaaaaat ataaaaatca tatttatata tgatatgtgt ccagacgatt
     1621 ttacaggcgg gaagattttc ccgcctgttt ttttctttat ttttttataa gataatcttt
     1681 tccggaaata ccaggttccg tcatatggat cggatccaga atgcgattaa gttcatcctc
     1741 gtccataagc ttttctttca gaatcagact tcttacagat tcgcctgtct taattgcctt
     1801 ttttgcaata tccgcagcct tctgatatcc aagatggggg ctgattgcag tgataatacc
     1861 tacactgttt tctaccagat aacggcatct ttcttcattt gccgtaattc ctacaataca
     1921 attatctacc aatgtctgga ctgcatagcc aagagtatcg atagactgaa acatgcaata
     1981 gaaaataatc ggctcaaacg catttaattc cagctgacca gcctctgcag ccatggtaat
     2041 ggtaacatca ttaccaataa tattaaaagc aacctgattc actacttcag gaataaccgg
     2101 attaacttta cctggcataa tagaggaacc attctgtttt gccggaagat taatctcccc
     2161 aaaaccagta cgtggaccgg aggacatcag acggagatca tttgacattt tggaaagagt
     2221 taccgcacat gctttcacag caccggaaac agctacaaat ggatccagat tctgagtgga
     2281 atcaatcaga tcaaaagcct gtataaagtc cattcccgat atttcagtga gatttggaac
     2341 aattcttctc agatagccct catctgcatt aattcctgtt ccaatagcag tacctcccat
     2401 attaagagtc cgcatttcat ccattgcttt atccattctg tggatatcac gcatgattgc
     2461 taccgaatat gccttaaatt cctgtcccag acgaattggt actgcatcct gcatctgtgt
     2521 acgccccatt tttattatgt gatcaaactc tgtagctttt tcattaagag catcatagag
     2581 gcgaagaagc tgttttttca gattctgcaa caaacgaaga gaagtcattt taccggcagt
     2641 aggaatcacg tcattggtag actgtccaca attcacatca tcgttaggat ttataattgt
     2701 ataatcgccc ttttttcctc ccagtatctc aatcgcgcga tttgcaatta cttcattggc
     2761 attcatattt aaagatgtgc cagctcctcc ctgaacagga tccacaataa attcattatg
     2821 gaattttcct gaaacaattt catcacatgc ctggacaatc gcctgtgctt ttttcttctc
     2881 aagaagcccc acctcacaat tggttatcgc tgctgctttt tttatgtatg caagactgtt
     2941 aataatttcc ggatgcatat tcagccccgt aatgtggaaa ttttcagccg cacgtaagga
     3001 ttgtactcca taatatacat cttccggaat atcctttaca ccaatagaat ccctttctac
     3061 acgatagtct gtttttttca tagttattcg ccctcccatg tatttgccct cattatatat
     3121 ctctgctata atataatcaa atacatatat ttttataaaa tatatagtgt ttaagttata
     3181 tatcggagga gtttatatgt ttcagggaat ggaatacatc tatgaggtct acaaggaaaa
     3241 aagcttttcc aaagctgctg ctgctctttt catttcgcag ccttctttaa gcgccaatgt
     3301 aaaacgtgtg gaaaaccgta ttggctatcc tatttttgat cgaagtacca aacctttgca
     3361 gttaactgaa gttggaaaac attatataca ggctgttgaa aaagtcatgg atattgaaaa
     3421 taatctcgaa aactacctac tggatctggg aaatctcaaa acaggaactc ttaatgtagg
     3481 tggcagtaac tttttttctt cg
//
LOCUS       DBMDXG010000217         3502 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6096_length_3502_cov_1.591591, whole genome shotgun sequence.
ACCESSION   DBMDXG010000217 DBMDXG010000000
VERSION     DBMDXG010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_3502_cov_1.591591"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="ACZPD3_06690"
     CDS             complement(<1..339)
                     /locus_tag="ACZPD3_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415720.1"
                     /translation="MKIAKKFMTLALAAALGVGCATVGVGAAPSNTSDLAAVAPSKTS
                     EITVDSEHSSIYEVVKELEKTQGFADLQAKYKDLADAFKKINDGTMKMEDFIKVLKSL
                     SVDEADKADLE"
     gene            complement(480..1517)
                     /locus_tag="ACZPD3_06695"
     CDS             complement(480..1517)
                     /locus_tag="ACZPD3_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR1415721.1"
                     /translation="MKMKKTTRKAIRQRKICMLLIIILIIVLSILGGGYYLLSQKNAT
                     SSKAGGQNSDSRNQTDLNQNSDTVEYKGETYKYNDHLSNYLFLGIDTREAVDTYQSQA
                     DAGQADAIFLVSMDRATEKIKVLFIPRDSMTRIEVFNPYGQSLGETTDHLNIQYAFGD
                     GKEKSCELMKTAVSDMLDGLPIQGYCSMNMDGISVITDFVGGIQLTIPDDSLADVNPE
                     YKKGAVVDITGETAEQFVRYRDIDKTQSALVRQERQKTFLQALVQKAQEKAGEDAGFV
                     TGLYDSVKSYTVTNMGNDIFAKLLAASQNGITDTETVPGEGTHGENFDEYHIDEDALS
                     DLIISMFYEKI"
     gene            complement(1548..2510)
                     /locus_tag="ACZPD3_06700"
     CDS             complement(1548..2510)
                     /locus_tag="ACZPD3_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415722.1"
                     /translation="MSKIRKKKIKKTNILLGFICILLAVLLGFILVTSYKSEQKESRH
                     LSEIADDQQSGIEDYEAVKERAKELEEISSEEAEDTEDIKSKNTEDTKDSEKTDTADT
                     EDADKTEDTKDKTTAASGIVCWGDDLINGEESNTYSYMTVLQKLLTDNGYNMTVINKT
                     LQGGGTLSMMKMAGVSDETIQGYITKHQQAANGAQLNVTETGIRDLTEEQTTRNDMDC
                     IPVIFMGYYGGWNHDPAELADQQEQILNTFQNKDQFIVVGTRPMDGSVTSEALDQVLS
                     QKWGEHYISLADVTAQPSSTYEAQQAMAEAILQKLQELNYISKN"
     gene            complement(2574..3284)
                     /locus_tag="ACZPD3_06705"
     CDS             complement(2574..3284)
                     /locus_tag="ACZPD3_06705"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HIR1415723.1"
                     /translation="MKKLNLTDKRNPDYFYSEAIKTLRTNIQLSGQSIKTILVTSCYP
                     NEGKSDIVLSLAQELGSIGKKVLLLDADIRKTAYAGRLGVEEEVKGLSQLLSGQVGLQ
                     EIIYSTNFPNMDIIFGGPSAPNPSGLLSENIFKVFLKEIREYYNYILIDTPPIGTVID
                     AAVIGRCCDGAVFLIEPGNVRYRDAQKAFKQLERSGCRILGAVMNKIDTSDDKYYSSY
                     YKHYGEYYHRSDEEAQIK"
     gene            complement(3306..>3502)
                     /locus_tag="ACZPD3_06710"
     CDS             complement(3306..>3502)
                     /locus_tag="ACZPD3_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HIR1415724.1"
                     /translation="LGFLLGFLACAAIVVVYAVMDDTIKTEEDIEKYLGVSVLAKVPD
                     RKDFINSKNRKSKNKNKKHH"
BASE COUNT          867 a          841 c          602 g         1192 t
ORIGIN      
        1 ttcaaggtct gccttgtctg cttcatctac agaaagggat ttcaatactt ttatgaaatc
       61 ttccatcttc attgtaccat cattgatttt tttaaaagca tctgccagat ctttatattt
      121 cgcctgcaga tcagcaaagc cctgtgtttt ctcaagttcc ttaacaactt cataaattga
      181 agaatgttca gaatccacag taatttcgct tgttttactt ggcgctaccg cggccagatc
      241 actcgtgttg cttggcgctg caccgacacc tacagtagca catcctactc caagggcagc
      301 tgctaaagca agtgtcataa atttctttgc gattttcata atgcacctct ccttttggat
      361 taatttttat atagataacc gtacagaatg tctgcacgga ctatctctga ataataacag
      421 tgataattat ttatcacagt acagtgtatt ctgtattcat catactatga ctaatacaat
      481 cagattttct cataaaacat ggaaataatc aaatctgaca atgcatcttc atcaatatga
      541 tactcatcaa agttctcccc atgtgttccc tcacccggta ctgtctctgt atcagtgatt
      601 ccattctgtg atgcagccag caatttggca aatatatcat tccccatatt tgtaactgta
      661 tagcttttaa cactgtcata gagacctgtc acaaatccgg cgtcctctcc tgctttttcc
      721 tgagccttct gcaccagtgc ctgaagaaag gttttctgtc tctcctgacg gaccagagca
      781 ctctgggttt tgtcaatatc acgatatctt acaaactgtt ctgcagtttc ccctgtgata
      841 tccacaacag cacctttttt atattccgga ttcacatctg cgagactgtc atcagggatt
      901 gtgagctgta ttccacctac gaaatctgta atcactgaaa tgccatccat gttcatggaa
      961 caatacccct ggatcggaag tccgtccagc atatcggaaa ctgctgtttt catcagttca
     1021 cagcttttct cttttccgtc cccgaaggca tactgtatat tcagatgatc tgttgtctct
     1081 ccaaggctct gaccatatgg attaaatact tctatcctgg tcatggaatc tcgtggaata
     1141 aacagaacct ttatcttctc tgttgcacga tccatggata ccagaaaaat tgcatctgcc
     1201 tgcccggcat ctgcctgtga ctgataagta tctacagctt cccttgtatc aattcccagg
     1261 aataaataat tgctgagatg atcgttatat ttgtatgtct cacctttata ttccacggta
     1321 tcactgtttt ggttcaaatc cgtctgattc cggctgtcac tgttctgacc accggctttt
     1381 gacgatgtag catttttctg gcttaacaga taataaccgc cgccaagaat ggaaagaact
     1441 atgatcagga taataatcaa aagcatacag atcttacgct gtctgatggc ttttcttgtg
     1501 gtttttttca ttttcattcc ttcaatctct gtatttcagt tatctcatca gtttttgctg
     1561 atataattaa gctcctgcaa tttctgtaat attgcctctg ccattgcctg ctgagcctca
     1621 taggtagaag acggctgtgc agtcacatct gcaagactga tatagtgttc tccccatttc
     1681 tggctgagca cctgatccag ggcttcagag gttacgcttc cgtccatagg gcgtgttcct
     1741 actacaataa actgatcttt attctgaaat gtatttaaaa tctgttcctg ctggtctgcc
     1801 agttctgcag gatcatgatt ccagccgcca taatatccca tgaagataac aggaatacaa
     1861 tccatatcat ttcttgtagt ctgttcttct gtcagatcac gtattccggt ctctgtcaca
     1921 ttcagctggg caccgtttgc agcctgctga tgtttagtaa tataaccctg aatggtttca
     1981 tcacttacac cagccatctt catcatggag agagtgccgc ctccctgaag cgttttgttg
     2041 atcactgtca tattatagcc attatcggtc agaagcttct gaagtactgt catataggaa
     2101 tatgtattgg attcttcccc attgatcaga tcatctcccc agcacacaat acctgaagca
     2161 gctgtcgtct tatcttttgt atcttctgtc ttatctgcat cttctgtgtc agctgtatcc
     2221 gttttttctg agtcttttgt gtcttctgtg ttctttgatt taatgtcttc tgtatcttct
     2281 gcttcttcgg aagatatctc ctccagctct tttgcacgtt cttttactgc ttcgtaatct
     2341 tcaatcccac tctgctggtc gtctgcaatt tctgacaggt gtctggattc cttctgttcc
     2401 gatttataac tggtcacaag aataaagccc aggagaactg ccagcagaat gcagataaag
     2461 cccagtaaga tattcgtctt ttttattttt ttctttctta ttttgctcac ttttccaaat
     2521 tctcctttat cattgatcat ggtttctgtc ttactgccag ataactgccc gtattattta
     2581 atctgggctt cttcgtcact tctgtgataa tactctccat aatgcttata ataagaagaa
     2641 taatatttgt catcactggt atctatcttg ttcataacag ctccaagaat acggcatccg
     2701 cttctttcca gctgtttaaa tgctttctgt gcgtcccggt acctgacatt tcccggttcg
     2761 atcagaaaga ctgcaccatc gcagcatctt cctatcactg ccgcatcaat tactgttccg
     2821 ataggcggtg tatcgatcag gatataatta taatattctc tgatttcctt taagaatacc
     2881 ttaaagatat tctcactgag aagtcctgac ggattcggtg cacttggacc tccgaatatg
     2941 atatccatat tcggaaagtt tgtagaataa ataatctcct gtagtcccac ctgaccactc
     3001 agaagctgtg agagaccttt cacttcctct tccacaccca gtcgtccggc atatgcagtc
     3061 ttcctgatat ccgcatccag aaggagaacc ttcttgccaa tgctcccaag ctcctgggca
     3121 agactgagta ctatatcact cttaccttcg tttggataac agcttgtaac cagaattgtc
     3181 tttatgctct gacccgacag ctgaatattg gtacgcagag ttttgatcgc ctcactgtaa
     3241 aaataatccg gatttctttt atctgttaaa ttgagtttct tcattgctca gattcctcca
     3301 tcagtttaat ggtgcttctt attcttgttc tttgatttcc tgttcttcga atttataaaa
     3361 tcttttctgt caggaacctt ggcaagaaca gaaactccca ggtatttctc aatatcttcc
     3421 tctgtcttaa tagtatcatc cattacagcg tatacaacca cgattgccgc acatgcaagg
     3481 aaaccgagta agaagccgag tg
//
LOCUS       DBMDXG010000218         3500 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6101_length_3500_cov_1.236540, whole genome shotgun sequence.
ACCESSION   DBMDXG010000218 DBMDXG010000000
VERSION     DBMDXG010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6101_length_3500_cov_1.236540"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            34..609
                     /locus_tag="ACZPD3_06715"
     CDS             34..609
                     /locus_tag="ACZPD3_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415725.1"
                     /translation="MFLQNSQKKIEEEFLKLTEKLGPDREPLKTICEKLDGCGRELGL
                     AMKYLYVTAPYSDLVNYSFEEIQDFASHGLFLYNTLERVKELPEEMFLNYILDHRVNE
                     EEVLPCRSFFWNELKDRIEGKNAKDAANEVNYWCAEEATYHSGDDRTLPALTVYRRGY
                     GRCRIFFAPSEWALSSRKKPFFFSCPIKIFW"
     gene            complement(510..908)
                     /locus_tag="ACZPD3_06720"
     CDS             complement(510..908)
                     /locus_tag="ACZPD3_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1415726.1"
                     /translation="MFDDPNVVLHCEKIDKIYPGTKALDQVSFDLLKGKVNVLIGENG
                     AGKSTLMKMIAGIEQPSAGKMYMDGQEVYFKDTNAARAKGIGIIHQELSLFPNMTVYQ
                     NIFMGHEKKKGFFLDDKAHSEGAKKILQRP"
     gene            complement(923..1510)
                     /locus_tag="ACZPD3_06725"
     CDS             complement(923..1510)
                     /locus_tag="ACZPD3_06725"
                     /inference="COORDINATES: protein motif:HMM:NF021565.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 family protein"
                     /protein_id="HIR1415727.1"
                     /translation="MKSRRILAVLAACTAVTFTGCGVVTVVPIGEEASYTGKQEFDSA
                     AESQGDWGAVVADISDKAQDLSELLNGDGITETIAVKGTGKIKEYNTDTPKHYLIVEL
                     DGYTGTTEVQVRTDGPNSSTAIRDLQSLKNFESFTNQTEWSSYGKELNKQALAQVIDP
                     LGIDENVVGKTVTFTGGAEAGTDAVSVTAVELTIE"
     gene            complement(1684..2667)
                     /locus_tag="ACZPD3_06730"
     CDS             complement(1684..2667)
                     /locus_tag="ACZPD3_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1415728.1"
                     /translation="MKARKAMALAMTAAMVGGLMTVPVMAEENAELLPGGGSKIIYCI
                     TPSTSNPFFNTEQVIAKEKAEELGYEVKCASHDDDAATQLELFEAAINDGAAAIICDN
                     AGADASIEAVQKAYDAGIPTFLIDREINQSGLAVAQIVADNAQGAAAIAEVWVEAMNY
                     EGKYAELLGLESDTNCQVRSDNYHSVIDEYEDLEMVAQQSANWDQTEAYEKTEAILQS
                     NPEITGIICGNDTMACGAVQACLDAGRNDIKIIGLDGSDEANAYIKSGDMVGTALQQI
                     ALITEMAVEQADAYLNGTAPEEEKQLVPCVAITADNTDCLNAFVYTEPEAK"
     gene            complement(3096..>3500)
                     /locus_tag="ACZPD3_06735"
     CDS             complement(3096..>3500)
                     /locus_tag="ACZPD3_06735"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR1415729.1"
                     /translation="DKLSTCDLSKVYLFLVNEIEGEQITGCKDKDQILDAMAAKFPNA
                     RFVLTLGSDGAVYYDGKEKVFQDIFKVKAVDTTAAGDTFTGYFIAAVIEGRSIQEALR
                     MAAKASSIAVSRPGATPSIPRADEVKKELGML"
BASE COUNT          893 a          777 c          752 g         1078 t
ORIGIN      
        1 cgttttttct atttaaagta ggaggatgaa tagatgtttc ttcagaacag tcagaagaag
       61 attgaagaag aatttttgaa attaacagag aagcttggac cggacaggga gccgctgaag
      121 acgatttgtg agaagctgga tggatgcggc agagaactgg ggctggcgat gaagtatttg
      181 tatgtgacag cgccttatag tgatcttgtg aattattctt ttgaggagat tcaggatttc
      241 gcaagccatg gactgtttct ttataatact ctggaacgtg tgaaagagct gccggaggag
      301 atgtttttga attatattct ggatcacagg gtgaatgagg aagaggtact tccgtgtcga
      361 agctttttct ggaatgagtt gaaggataga attgaaggaa aaaatgcgaa agatgcggca
      421 aatgaggtaa attactggtg tgcggaggaa gctacttatc atagtggtga tgaccgtaca
      481 cttccggcac ttacggtata tcgaagaggt tacggacgct gcaggatttt tttcgccccc
      541 tctgaatggg ccttgtcatc caggaaaaag cctttctttt tctcatgtcc cataaaaata
      601 ttctggtaaa ctgtcatatt cgggaaaaga gaaagttcct ggtggatgat tccgatacct
      661 tttgcacggg ctgcattggt atccttgaag taaacctcct ggccgtccat gtacatctta
      721 cccgctgaag gctgctcgat tccggcaatc atcttcatca gggtggattt tccggcaccg
      781 ttctcaccga tcagaacgtt tacctttcct ttcagaagat cgaaggaaac ctgatccaga
      841 gctttggttc cgggataaat tttatcaatt ttttcacaat ggagaacaac attcggatca
      901 tcaaacataa tgcttcctcc ttttattcaa tcgtcagctc aactgctgta acagatactg
      961 catctgtacc ggcttctgcg ccgcctgtaa atgtaacagt cttgccaact acattttcgt
     1021 cgattccaag tgggtcaatt acctgtgcaa gcgcctgctt gttcagttct tttccatagc
     1081 ttgaccattc cgtctggttg gtaaagcttt caaaattctt cagagactga aggtcacgga
     1141 tggcggtact ggagttcggt ccatctgtcc ggacctgaac ctctgtagtt ccggtatatc
     1201 catccagttc tactatcaga taatgtttcg gggtgtctgt attgtattct ttgatttttc
     1261 ctgtgccttt tactgcaatt gtttctgtaa tgccatcacc atttaataat tcagaaagat
     1321 cctgtgcctt gtcggaaatg tccgctacaa cggctcccca gtcaccctgg gactctgctg
     1381 cggaatcaaa ttcctgtttt cctgtataag aagcttcttc gccaatgggt actacagtta
     1441 caacaccaca gccggtaaag gttactgctg tacatgcggc cagaactgcc aaaattcttc
     1501 tgcttttcat aatcatctct ccctgctttc tattataaaa ggcggcgctg gcgcaccgcc
     1561 ttccaaagcg tgtttaaaaa gactttctgc aaaatatacc acaaagcggg gcatgcagat
     1621 tgtgcgaata atttttcaaa cactcgttca ttttttataa aaatctgttt cctggcagcc
     1681 agattattta gcttctggtt ctgtgtaaac aaatgcgttc aggcaatctg tgttgtcagc
     1741 tgtgattgct acacaaggaa caagctgttt ctcttcttcc ggagctgtac cgttaaggta
     1801 agcgtctgcc tgctcaactg ccatctcagt gatcagagcg atctgctgaa gagcggtacc
     1861 aaccatgtca ccagatttga tgtaagcgtt tgcttcgtca gatccgtcaa gaccgatgat
     1921 cttaatgtcg ttacgtcctg catccagaca agcctgaaca gcaccacatg ccattgtgtc
     1981 attaccgcag ataataccgg tgatctctgg attggactga aggattgctt ctgtcttctc
     2041 ataagcttct gtctgatccc agttagcgga ctgctgagct accatctcaa gatcctcata
     2101 ctcatcaatt acagaatggt agttatcaga acgaacctgg cagtttgtat cggattcaag
     2161 tccaaggagc tctgcgtatt tgccttcgta gttcatagcc tcaacccata cttctgcgat
     2221 tgctgctgca ccctgtgcgt tatctgcaac gatctgtgct actgcaagtc cggactggtt
     2281 gatttcacgg tcgataagga aagtcgggat tccagcgtca tatgctttct gaactgcttc
     2341 gatggaagca tctgcaccag cgttatcgca aatgattgct gctgcgccat cgttgatagc
     2401 tgcttcgaaa agctcaagct gtgtagcagc gtcgtcatcg tgggaagcac attttacttc
     2461 gtatccaagt tcttcagctt tttctttcgc aattacctgc tctgtgttga agaatgggtt
     2521 agatgtggaa ggtgtgatac agtaaatgat cttgcttccg ccgcctggaa gtaattctgc
     2581 attctcttca gccattaccg gaactgtcat taaacctcca accatcgctg ctgtcatagc
     2641 caatgccatt gcttttcttg ctttcataaa tacgtcctcc ttgaaatata attaagatct
     2701 gtttttgttc ggttttctct ccgaactgtt gataatttga ttataaacgt ttgcataaat
     2761 atttcaataa tctttttagt tttatgtgtt attttgtatg aatgcacaag gtttttgttg
     2821 tgattttagt aagtttttac tataaaatct atgtattccc aaccatctat tagtgcattt
     2881 atgtaaacgt ttatataaac atttataaac agtatgtaaa acataatata aattttgcgc
     2941 gttatggaga cggttgccaa gactatccag agccaggcaa cagctctggt gaataagacc
     3001 tgttctctgc aaaaataagg cagtcaccaa ggtctcatgc aaacatggac tttggtgact
     3061 gccacacaca gatacacgct tatgtaattt gaaatctaca acattcccag ctctttcttc
     3121 acttcatccg ctctcggaat ggacggtgta gcgcctggtc ttgaaacagc aatggaggag
     3181 gcctttgcag ccattctcag cgcctcctgg atggatctgc cctcgatgac tgcagcaatg
     3241 aaatatcctg taaatgtatc acccgccgct gtggtatcca cggcttttac cttgaaaatg
     3301 tcctggaaaa ctttttcttt tccatcgtaa taaacagcgc cgtcacttcc aagtgtaagc
     3361 acgaaacgtg cattaggaaa tttcgcagcc atggcatcca ggatctgatc tttatccttg
     3421 cagccagtga tctgttcacc ttcaatttcg tttacgagaa acagataaac cttactgaga
     3481 tcacaggtgc tcagcttgtc
//
LOCUS       DBMDXG010000219         3498 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6106_length_3498_cov_1.350898, whole genome shotgun sequence.
ACCESSION   DBMDXG010000219 DBMDXG010000000
VERSION     DBMDXG010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6106_length_3498_cov_1.350898"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            494..880
                     /locus_tag="ACZPD3_06740"
     CDS             494..880
                     /locus_tag="ACZPD3_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR1415730.1"
                     /translation="MATATIPLKGSASMESKKVSISSKRQITIPQKFFTLLGFNTEAE
                     CIMRGNELVLRPVKENTSGEFAEQILADLIRQGYSGEELLEKFKQTQRKIRPAVEAML
                     AEADRVAESKSGGYSLEDVFGTEDEK"
     gene            877..1173
                     /locus_tag="ACZPD3_06745"
     CDS             877..1173
                     /locus_tag="ACZPD3_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR1415731.1"
                     /translation="MTQVQILPPAAKFLKKLKDKKLKSLYKEAIEMICEDYSIGEEKT
                     GDLAGMYGYDIYYNKTNYELAYRVRQLDDLIIIIIMAGTRENFYEELKRYIRTI"
     gene            1389..2978
                     /locus_tag="ACZPD3_06750"
     CDS             1389..2978
                     /locus_tag="ACZPD3_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR1415732.1"
                     /translation="MGRFVNSGNSAFQVALNSEIYVDKTGLLAYTNKVMDTTAKFICN
                     SRPRRFGKSITADMLTAYYCKGCSSEEMFSSLEIGQAPGFTKYLNQYDVIHLDIQWCM
                     EPAGGPENVVSYITEKTILELRNYYPEILPDSISSLPEALSCINTATGQKFVVIIDEW
                     DVLIRDASTDLVVQEEYINFLRGMFKGSEPTKYIQLAYLTGILPIKKIKTQSALNNFA
                     EFTMTDARIFSRYIGFTEEEVRMLCEKYHRDFSKTKHWYDGYLLEQYQVYNPKAVVEL
                     MIWNKFQSYWSDTGTYEAIVPLINMDFDGLKTAIIEMLSGNSVEIDPTTFQNDMVNFT
                     CRDDILTCLIHLGYLGYDQDTHSAFVPNEEIRQELAKAVKRKKWNEFLTFQQESSELL
                     DATLDMDADTVARSIEKIHNEYASAIQYNNENSLSSILSIGYLSTMRYYFKPIREFPA
                     GRGFADFVYLPKPEYSRDYPALVVELKWNQNAQTAIRQIKNRQYPEAVTNYTDNILLV
                     GISYDKKQKTHNCVIEKYSEK"
BASE COUNT         1154 a          726 c          618 g         1000 t
ORIGIN      
        1 tcagaaccag aaaactttcc accgcatcat tcatcagata aatcaaacgt aattctccac
       61 tctccgtctg tccggtaaaa ctatctggaa tatccacaaa ttcacactgt tcctgctcca
      121 gaagcagatt taatttttca aaattgtctt ccattatatc ttccatcctt aactcctgct
      181 gtttctgtac aatgtctttc gccgattctg tttaacacac ttgataaaag aatccattgc
      241 atcgtttcat ggaattaccg caatgcagaa gcaggataca tttgcacaat tttacagata
      301 tatttttatg atatcactaa tcatattttc tgccaaatag aaaaattttt ctattcaata
      361 aagaaactga ttttacttct ctgtaattga catcccaaac ataatatttt acaatataac
      421 aaagaacaaa gaaaccaagc agtatgtata ttacagaata aacaaacata aaataggaag
      481 gaggcataca cgaatggcaa cagcaacaat cccattgaaa ggcagcgcat ccatggaatc
      541 aaaaaaagtc agtatttcat ctaaacgcca gattactatc ccgcagaaat tttttacgct
      601 tctgggtttt aatacggaag cagaatgtat catgcgtgga aacgaactgg ttttacgccc
      661 cgttaaagaa aataccagtg gtgaatttgc cgaacagatt cttgccgatc tgatcagaca
      721 gggatatagc ggtgaagaat tacttgagaa atttaaacag acacagcgaa aaatccgtcc
      781 ggcagttgaa gcaatgcttg ccgaagcaga ccgggtcgca gaatctaaat caggaggata
      841 ctccttagag gatgttttcg gaacggagga tgaaaaatga cacaggtaca gatccttcca
      901 cctgcagcaa aatttctgaa aaaacttaaa gataaaaagc tgaaatctct gtacaaagaa
      961 gctattgaaa tgatatgtga agattattcc atcggcgaag aaaagacagg tgatctggca
     1021 ggaatgtacg gttatgacat ttattataat aaaactaatt atgaactggc atatcgggtg
     1081 agacagcttg atgatttgat aatcatcatt atcatggccg gaaccagaga aaatttttac
     1141 gaggagttaa aacgttatat acgcactata taacagtaca tatttgtatc acaagataat
     1201 cccccggaag ttaatcagta cttccagggg attattctat tgacaaaatt tattcctgcg
     1261 ctcccgtatc agatgtcaga tatctttgcc atgtctcatt gacattctga atatagcatt
     1321 ttacaataga acaaagaaac caatcagtat acatgataaa aaacactgaa cagaaaggag
     1381 tcatttccat gggacgattt gtaaattcgg gaaacagcgc ttttcaggtt gcgctgaatt
     1441 cagaaatcta tgtagataaa accggtttac tggcatatac aaataaggtt atggacacca
     1501 ctgcaaaatt catatgcaac agtcgccccc gtaggttcgg gaaatccatc actgccgata
     1561 tgctcaccgc atattactgt aaaggctgta gttccgaaga aatgttctca tctctggaaa
     1621 tcggtcaggc acctggtttc acgaaatatc tgaatcaata tgatgtgatc catctggata
     1681 ttcaatggtg tatggaacca gccggcggtc cggagaatgt agtttcctac atcactgaaa
     1741 aaacgattct ggaattaaga aattattacc ctgaaattct gcctgactcc ataagctctc
     1801 tgcctgaggc tctgtcctgc attaacactg ccactggtca aaagttcgta gtaattattg
     1861 atgaatggga tgttttaatc cgagatgcct ctaccgacct ggtagtccaa gaggaataca
     1921 tcaactttct ccgcggtatg ttcaaaggtt ctgaacctac caaatatatt caactggctt
     1981 acctcaccgg gattcttcca atcaaaaaaa tcaaaacaca gtcagctctg aacaattttg
     2041 cggagtttac aatgactgac gccaggatct tttccagata tattggcttt acggaggaag
     2101 aagtacgaat gctctgcgaa aaataccacc gggatttctc caaaaccaag cactggtatg
     2161 acggttatct gctggagcaa tatcaggtat acaatcctaa agcagtagta gaattaatga
     2221 tctggaataa attccaaagc tactggtcgg ataccggaac ctacgaagca attgtgcctc
     2281 tgatcaatat ggattttgac ggactcaaaa ctgccattat agaaatgttg tccggtaatt
     2341 ccgtagaaat cgatcctacg actttccaga atgatatggt gaactttacc tgcagagatg
     2401 atattcttac ctgcctgatc catctgggat atctgggtta tgatcaggac actcattctg
     2461 catttgttcc aaacgaagaa atccgtcagg aacttgccaa agctgttaaa cgaaagaaat
     2521 ggaatgaatt tctgacattc cagcaggaat cctcagagtt gctggatgcc actctggaca
     2581 tggacgcaga cactgtggcc agatccattg aaaaaataca caatgaatac gcttcagcta
     2641 ttcagtacaa caacgaaaac tccctcagca gcattctgtc catcggttat ctcagcacta
     2701 tgcggtatta ttttaaacct atacgtgaat ttcctgcagg cagaggtttt gcagattttg
     2761 tctacctccc aaaaccagaa tattccagag attaccctgc acttgttgtt gagttaaaat
     2821 ggaatcaaaa tgcccagacc gcaatccggc agataaaaaa caggcagtac cctgaggctg
     2881 ttacaaatta tactgacaat attttacttg ttggaatttc ttatgataaa aaacagaaaa
     2941 cacataattg tgtaatcgaa aaatactctg agaaataata aaggggctga ttgtccttta
     3001 cgaagaacat cttaccgatt atctatacga gaaaagaaat gcaggaatgg gaagctcttg
     3061 taagctaacc cattcccact ctctgtctat tccttcgtca tttgtattat taccagaagt
     3121 ttcctgatac ttccaatcac tttatatatg tccataatga ttttcagaca gtcattctta
     3181 taatattttt cttctcccat ggaaacaata ccgctttcac atatattcta tattcagcgc
     3241 ttgaaagtac caggaattaa taatttttca cgcacaaaat ctttaaaatc gcttctttgt
     3301 ttccatatcc tgattgtatc acacagactc ccgaaatcgc atgcattcac acaaacaact
     3361 atatttcact acatatttaa ccgatttttt ccaatcttcc tcacagacaa attgcatccc
     3421 gttatttttt atagtatgat tattggaaat acagttcatt ttatccatct ggcatatccg
     3481 acagatattg tcactgac
//
LOCUS       DBMDXG010000220         3494 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6114_length_3494_cov_2.157088, whole genome shotgun sequence.
ACCESSION   DBMDXG010000220 DBMDXG010000000
VERSION     DBMDXG010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_3494_cov_2.157088"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(340..492)
                     /locus_tag="ACZPD3_06755"
                     /pseudo
     CDS             complement(340..492)
                     /locus_tag="ACZPD3_06755"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            451..555
                     /locus_tag="ACZPD3_06760"
                     /pseudo
     CDS             451..555
                     /locus_tag="ACZPD3_06760"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            599..2395
                     /locus_tag="ACZPD3_06765"
     CDS             599..2395
                     /locus_tag="ACZPD3_06765"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HIR1415733.1"
                     /translation="MAKSRLIGSYPVIGIRPTIDGRRGALDVRGSLEDQTMNMAKSAA
                     KLFEENLRYSNGEPVKVVIADTTIGRVGESAACADKFRKEGVDITLTVTPCWCYGAET
                     MDMDPQTIKAVWGFNGTERPGAVYLASVLATHAQKGLPAFGIYGHDVQEADDTSIPED
                     VKEKLLRFGRAAVAAASMRGKSYLQIGSVTMGIGGSIIDSDFIESYLGMRVESVDEVE
                     IIRRMTEGIYDHAEFDKALKWAKETCKIGWDKNPEELQFSAEKKEEQFEFVVKMAVII
                     KELMNGCDNLDPKFSEEAIGHNALAAGFQGQRQWTDFYPNGDFAEAMLNTSFDWNGAR
                     EPYILATENDVLNGLGMLFMKLLTNRAQIFADVRTYWSPEAVKKATGYDLEGVAKEAG
                     GFLHLINSGAACLDANGEAKEADGTAVMKQWWDITEEDQKAIMENTEWCMADNGYFRG
                     GGYSSRYETRAQMPATMIRLNLVKGLGPVLQIAEGWTVALPEEVSDKLWKRTDYTWPC
                     TWFAPRCDGKEGPFKTAYDVMNNWGANHGAISYGHIGADLITMCSMLRIPVSMHNVPE
                     KDIYRPAAWNAFGMDKEGADFRACKNYGPLYK"
     gene            complement(2614..3057)
                     /locus_tag="ACZPD3_06770"
     CDS             complement(2614..3057)
                     /locus_tag="ACZPD3_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HIR1415734.1"
                     /translation="MLKGIPEILSPELLKVLCEMGHSDRLVIADGNFPVESMGKNAIT
                     IRCDGHGVPEILDAILKLFPLDTYVEHPVNLMEVMPGDNVETPIWDTYKEIVAKYDER
                     GDKAVGNIERFAFYEEAKTAYCIISTSEKALYANIMLQKGVVINN"
BASE COUNT         1099 a          649 c          802 g          944 t
ORIGIN      
        1 cagtgataag acatgacata tctgggatgt gtatgatcga tgtgatgaaa ctcaaatgga
       61 aactcataag tgcctcttgg acggtcttca ttatggttta aatcatgcaa aagaactttc
      121 ctccttacaa aatttacaga aattttatga ttaaaacaga aaaagtgaat attgtactgt
      181 tttttgctat tataacacaa gtaattttct gaatgcaatt atataataga tatcagtaac
      241 aaagttgagt ggtcaggctg ctgtgaacgg agtgaacagt ggttatcggg ctacttggca
      301 caacaggtgt agaggttatt atttttgtta tatttgcagt cagaagatgc tgcgaaaagt
      361 gaaaatgtaa tcttgggggt tagagcgtgt ctgaaaaatc attcccgcaa tctgcacgcc
      421 ccactttgcg ggatatttca cccgaattca gttgccgtag cccgctacgg cgccctcatc
      481 cgggcaaaat atcccacaaa ttgtgacgca catcttgcag aaagcctttt tcagacacgc
      541 tctagcagga aatgatcctg cgggaatatt acaataattt atcaggaggt attttattat
      601 ggcaaagagc agattaattg gcagctatcc ggtaattggt atcagaccta ctatcgatgg
      661 tcgtagagga gcactggatg ttcgtggatc tctggaagat cagacaatga atatggcgaa
      721 atcagctgct aaattatttg aagagaattt aagatattcc aacggtgaac cggttaaagt
      781 tgtaatcgct gatacaacca tcggacgtgt tggagagagc gctgcatgtg cagataaatt
      841 ccgtaaagaa ggcgtagata tcactcttac agttacacca tgctggtgct acggcgctga
      901 gacaatggat atggatccac agacgatcaa agctgtatgg ggattcaacg gaacagaaag
      961 acctggtgct gtataccttg catctgtact tgcaacacat gcacagaaag gacttccggc
     1021 attcggtatc tatggacatg atgttcagga agctgatgat acatctattc cggaagatgt
     1081 taaagaaaaa ctgttaagat tcggccgcgc agcagttgca gcagcatcca tgagaggaaa
     1141 atcctatctg cagatcggtt ctgtaacaat gggtatcggc ggatctatca ttgattccga
     1201 tttcatcgaa tcttatcttg gaatgagagt tgagtctgtt gatgaggtag agatcatccg
     1261 tcgtatgaca gaaggtatct atgatcacgc agaatttgat aaagctctga aatgggcaaa
     1321 agaaacctgt aagattggct gggataagaa ccctgaagaa cttcagttct cagctgaaaa
     1381 gaaagaagag cagttcgagt ttgttgttaa gatggcagtt atcatcaaag aactgatgaa
     1441 cggctgtgac aaccttgatc ctaaattctc tgaagaagca atcggacaca atgctctggc
     1501 agccggtttc cagggacaga gacagtggac agatttctat ccgaacggtg actttgcaga
     1561 agctatgctg aacacctcct ttgactggaa cggagcacgt gaaccataca tcctggctac
     1621 agagaacgat gttcttaacg gacttggaat gctgttcatg aaacttctta caaacagagc
     1681 acagattttc gctgacgttc gtacatactg gagcccggag gctgttaaga aagctacagg
     1741 atatgacctt gagggcgtag caaaagaagc cggcggtttc cttcacctga tcaactccgg
     1801 tgctgcctgc cttgatgcta acggagaagc aaaagaagct gatggaacag ctgttatgaa
     1861 acagtggtgg gatatcacag aagaagacca gaaagcaatc atggaaaaca cagaatggtg
     1921 tatggctgac aacggatatt tccgtggagg cggatactcc agccgttatg aaacacgtgc
     1981 ccagatgcct gcaacaatga tccgcctgaa ccttgtaaaa ggacttggac cggttctgca
     2041 gatcgctgaa ggatggacag tagctcttcc tgaagaagta tctgacaagc tgtggaaacg
     2101 tacagactat acatggccat gtacatggtt tgctccaaga tgcgatggca aagaaggacc
     2161 gttcaagaca gcttatgatg taatgaacaa ctggggtgca aaccatggag caatctccta
     2221 cggacacatt ggtgcagatc ttatcacaat gtgctctatg ctgagaattc ctgtatccat
     2281 gcacaatgtt cctgagaaag atatctatcg tccggcagca tggaatgcat ttggaatgga
     2341 taaagaaggt gcagatttca gagcatgcaa gaattatgga cctctttaca aataataaat
     2401 aaaaccgata tgtaagaatg tatatcggcc gcccggagtt ggagatgatc tgactttggg
     2461 cggctgtttg tatcaggggg aatcaaaaaa acaagccgat atctgtgtgt acagatacca
     2521 gcctgttgta tatatgtatg tgtgatgtgt gtacaatgtg taaaaatgat atttggcatg
     2581 aatatgccaa aatcccgaaa agataaagca aaatcagttg ttgattacaa cacccttctg
     2641 aagcattata tttgcataca gagctttttc gcttgtggaa ataatgcaat atgcagtttt
     2701 ggcttcttca tagaaagcga aacgttcaat atttccaacc gctttatcgc cgcgctcgtc
     2761 atatttggca acgatttctt tatatgtatc ccagattggt gtttctacat tatcgccagg
     2821 cataacttcc atgagattta caggatgctc tacataagta tccagtggaa acagtttgag
     2881 gattgcatcc aggatttccg gtacaccatg tccgtcacag cggatagtga tggcattctt
     2941 tcccatagat tcaacaggga agttaccgtc tgcgataaca agacgatcgc tgtgtcccat
     3001 ttcacataaa acttttaata attccggtga taaaatttca ggaattccct ttaacataat
     3061 tcgtattctc ctttcaaaat tctgaaaatc attgtaaaca gtttaatgaa aaaaatcaga
     3121 ttattcaagg caatatttta cgcaaaaaga gtaaattttt atagtttaca gcggaggaaa
     3181 cagtaacaca cagagttgtg cgaaatacac aaaaatatat tgaaaaaatt gaaaaaatag
     3241 tataaatatg ctattatcat tatagcgaat cacgcagata ttcgaaacct gcgttactgc
     3301 tcgcttataa ccgaatagct gctttgcgga ggttttacct gattcggtta aataagtaaa
     3361 aggaagtagt aatttttatt gggttatcag gcaggggatg atgcctgatc ttatggggga
     3421 taaaacttaa tatgtacaaa atattattgg tagacgacga aatcctggtc agggatgcaa
     3481 tcagggagaa catt
//
LOCUS       DBMDXG010000221         3489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6124_length_3489_cov_1.524793, whole genome shotgun sequence.
ACCESSION   DBMDXG010000221 DBMDXG010000000
VERSION     DBMDXG010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6124_length_3489_cov_1.524793"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="ACZPD3_06775"
     CDS             complement(<1..1417)
                     /locus_tag="ACZPD3_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR1415735.1"
                     /translation="MDVKNHNTNRKKRVVRKRWNAAVLIALFVGILLTVFQYLGFVSK
                     TVYEESVSHLTEIFHQSDNMLSELTNKNLTYLHIWGEYLQNTSDESEIREYIEKAQED
                     AGFLDFYFLSADGNYKMATGETGYLGLQENIEDEIRQGNDVITNATVPGKSQLLVFAT
                     PRPHGSYQGFEYDAIAIAYENSDIVNVLNISAFNGNAQSYVVHPDGRVVVDHSSEAWG
                     EVYNFFGILREHSNMSEKEILKLSEEFKEGHTDAMLINLDGEDYYLVYEKSKPQDWIF
                     LGLVQADIVNASMNVLQRSTVLLVSAVAVCIAGLFIGIILRKNRVNLKRKDTEILYRD
                     ELFQKLSMNVDDVFLMLDAKTYQADYVSPNVEKLLGITVEQIRKDICVLGKLHPGDVE
                     DPEKKYLEEIQVHEQQEWDLEYVHQKTGEHRWFHNVAMGSEVNGKKKYILVLSDRTSD
                     RKMNQALSEAVRAAETANKAKS"
     gene            complement(1423..3273)
                     /locus_tag="ACZPD3_06780"
     CDS             complement(1423..3273)
                     /locus_tag="ACZPD3_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR1415736.1"
                     /translation="MKKKKWNKLVSVLLAMVTAVSLLSGCGGKSAEKEDAETITVYLW
                     STKLYEKYAPYIQEQLPDINVEFVVGNNDLDFYRFLNENGGLPDIITCCRFSLHDASP
                     LKDSLMDLSTTNAAGAVYDTYLNNFKNQDGSVNWLPVCADAHGFVVNKDLFEKYDIPL
                     PTDYESFVSACQAFDEVGIRGFTADYYYDYTCMETLQGLSASELSSGDGRKWRTIYSD
                     PDNTKREGLDSTVWPEAFERMEQFIQDTGLSQDDLDMNYDDVVEMYKSGKLAMYFGSS
                     AGVKMFQDQGINTTFLPFFQQNGEKWLMTTPYFQIALNRDLTKDETRRQKAMKVLNTM
                     LSEDAQNRIIYDGQDLLSYSQDVDFRLTEYLKDVKPVIEENHMYIRIASNDFFSISRD
                     VVSKMISGEYNAEQAYQSFNSQLLEEKSTSEDIVLDSKKTYSNRFHTSGGNEAYSVMA
                     NTLRGIYGTDVLIATGNSFTGNVLKAGYTEKMAGNMIMPNELSAYSSEMSGAELKETV
                     RNFVEGYQGGFIPFNRGSLPVFSGISVEIRETDNGYTLSKVSKNGKQIQDKDTFTVTC
                     LAAPQYMEAYPAEENIVFDGGDTSVEDTWTTYVSDGNAILAEPEDYITLR"
     gene            complement(3273..>3489)
                     /locus_tag="ACZPD3_06785"
     CDS             complement(3273..>3489)
                     /locus_tag="ACZPD3_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1415737.1"
                     /translation="NGYEAAKMIRSLDREDAKVIPIIAMTANAFTEDKMRAKEAGMDE
                     HIAKPVDGKLLVKVINELVKRDQREKL"
BASE COUNT          930 a          881 c          618 g         1060 t
ORIGIN      
        1 tgctctttgc cttgtttgca gtctccgcag cgcggaccgc ctcagaaagg gcctgattca
       61 tttttctgtc agaggtacga tctgacagaa ccaggatata tttctttttc ccgtttacct
      121 cgctgcccat tgcaacgtta tgaaaccagc ggtgttctcc tgttttctga tgaacatatt
      181 caagatccca ttcctgctgc tcatgaacct ggatttcttc caaatacttc ttctccggat
      241 cttcaacgtc cccgggatgc agttttccaa ggacacaaat atctttacga atctgttcta
      301 ctgtaattcc cagcagtttt tccacattgg gactgacgta atctgcctga tatgtttttg
      361 catccagcat caggaaaacg tcatctacat tcattgacag cttttgaaac aattcgtccc
      421 tgtacaggat ctccgtatcc tttctcttca ggtttaccct gtttttccgg agaataattc
      481 caataaaaag accggcaata cacaccgcta ctgcacttac aagcagcaca gtgctacgct
      541 gcagaacatt catacttgca ttgacgatat cagcctggac aagtcccaga aagatccagt
      601 cctgaggttt ggatttctca tagaccagat agtaatcctc tccatccaga tttatcagca
      661 ttgcatctgt atgtccttcc ttaaactctt ctgaaagttt aaggatctct ttttcagaca
      721 tattggaatg ttctctcaga atcccaaaga aattatacac ctctccccat gcctcagaag
      781 aatgatccac aacaacacgg ccatccggat gaaccacata actctgggca tttccattga
      841 aagcagaaat attcagcaca tttacgatat cagagttttc ataggcaatg gcaatggcat
      901 catattcaaa gccctgataa cttccatgag gcctgggagt agcaaaaaca aggagctggg
      961 attttccggg aaccgttgca ttggttatca catcatttcc ctgccggatc tcatcttcaa
     1021 tattttcctg caatcccaga tacccggttt cacctgttgc catcttatag ttcccgtccg
     1081 ctgacaggaa ataaaaatct aaaaagcctg catcttcctg tgctttctct atatactcac
     1141 gaatttcact ttcatcggaa gtgttctgca aatattcccc ccatatatgg agataagtca
     1201 gattcttatt tgtcagctca cttagcatat tatcagactg atgaaaaatt tctgtcaggt
     1261 gagaaacact ttcctcataa acagtcttcg atacaaaccc caaatattgg aaaacagtca
     1321 aaaggattcc aacaaatagg gcaataagaa ctgctgcatt ccatcgtttt ctcacaaccc
     1381 gtttctttct gtttgtatta tgattcttaa catccattct tctcacctca gagtaatgta
     1441 gtcttcaggc tctgcaagaa tggcattacc atctgaaaca tatgttgtcc aggtatcttc
     1501 cacagaggta tcccctccat caaatacaat attttcctct gccggataag cttccatata
     1561 ttgtggtgct gcaaggcagg tcacagtaaa agtatccttg tcctgaatct gttttccatt
     1621 ctttgaaact ttactcaatg tataaccatt atccgtttcc ctgatttcta cagaaatacc
     1681 actgaacaca ggcagagagc cacggttaaa ggggataaag cctccctggt agccttctac
     1741 gaagtttctg accgtttcct tcagttctgc cccgctcatc tcactgctgt aagcggaaag
     1801 ctcgtttggc ataatcatgt tgcctgccat tttttctgta taaccggcct tcagtacatt
     1861 tcctgtaaag ctgtttcctg ttgcaatcag cacatccgtc ccataaatac cacgcaaagt
     1921 gtttgccata acagaatagg cttcattgcc tccgctggta tggaaacgat tggaataagt
     1981 tttctttgaa tccaacacaa tatcctcaga agtggatttt tcttcaagaa gctgggaatt
     2041 aaatgactga taagcctgct ctgcattata ttctcctgag atcatcttag acaccacatc
     2101 cctagaaatg gaaaaaaagt catttgacgc aatacggata tacatatggt tttcttcgat
     2161 cacaggtttc acatccttca gatattccgt aagccggaaa tccacatcct ggctatagct
     2221 taacagatcc tgcccatcat aaatgatccg gttctgcgca tcctctgaaa gcattgtgtt
     2281 cagtaccttc attgctttct gccgacgtgt ttcatccttt gtcaggtcac ggtttaaagc
     2341 tatctggaaa tatggtgtag tcatcagcca cttttcgccg ttctgctgaa agaatggcag
     2401 gaatgttgtg ttaatgccct gatcctggaa catttttaca ccggcagaac tgccgaaata
     2461 catggcaagc ttaccacttt tgtacatctc aaccacatca tcataattca tatccagatc
     2521 atcctggctc agccctgtat cctgaataaa ctgctccata cgttcaaaag cctccggcca
     2581 gacggtactg tccagacctt ctctctttgt attgtccggg tcactgtaga tggtacgcca
     2641 cttacgtcca tccccagaag acagctctga tgcagacaga ccctgcagtg tttccataca
     2701 ggtataatca tagtaatagt ctgcagtaaa cccacggata cccacctcat caaatgcctg
     2761 gcaggcagaa acgaagcttt cataatccgt tggaagagga atatcatact tttcaaaaag
     2821 atctttgttc actacaaagc catgggcatc tgcacacaca ggaagccagt ttacgctgcc
     2881 atcctgattt ttgaaattgt taaggtaggt atcatagaca gcacctgcag cattggttgt
     2941 ggaaagatcc atcagactgt ccttcagggg gcttgcatca tggagggaaa acctgcagca
     3001 ggttataata tccggcaatc cgccgttttc attcagaaaa cgatagaaat ccagatcatt
     3061 attgcctact acaaattcaa cgttgatatc cggaagctgt tcctggatgt atggcgcata
     3121 tttttcatac agctttgtgc tccataaata cactgtgatg gtttctgcat cttctttttc
     3181 cgcacttttc ccgccgcagc cagacagaag tgatacggct gtcaccatcg ccaaaagcac
     3241 agaaactaac ttgttccatt ttttcttttt cattataact tctccctctg atcgcgtttt
     3301 actaactcat tgatcacttt tactaataat ttcccatcaa caggcttggc aatatgttca
     3361 tccattcctg cttcctttgc tctcattttg tcctcagtga acgcgtttgc tgtcatggca
     3421 atgatcggta tcacttttgc atcctctctg tccagagagc ggatcatctt tgctgcttca
     3481 tatccattc
//
LOCUS       DBMDXG010000222         3483 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6140_length_3483_cov_1.728859, whole genome shotgun sequence.
ACCESSION   DBMDXG010000222 DBMDXG010000000
VERSION     DBMDXG010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6140_length_3483_cov_1.728859"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="ACZPD3_06790"
     CDS             complement(<1..1262)
                     /locus_tag="ACZPD3_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HIR1415738.1"
                     /translation="MTYSKKRTLSYGVILISVLLAYFCRQVRTENVFMRNLADQCRSC
                     IYLGMYCAWVIYLGKHVVHKKTRRCLTAIGCLMVFWFFVRTVKFHIFHDPLGEHICWY
                     LYYIPMLLIPVLGLAAAMFLGEKEEEKTVRKVIILLTVAAILIVSVFTNDLHQLVFRF
                     SKQPPFSDEDYSYGIVFMVIQGWILICLTGMEIILIRKSRIPGKKQFWLPVIPGILLL
                     GWNIGNILRLPFIKIIAGDMTAVCCLLMAAIFQGCILCGLIQTNNRYFELFQTSGGLD
                     AEITDHSFQRYYHSGDFPELSPELRKIIIDRSSVQEQGIRINHIPIRGGHLFWSEDIS
                     VLLDQYQDIREQQEELTARNRLLQKAYQKEAERRKTEEQNRLLNMIQNQTAGQLELLS
                     QFMDELERTESREQYDRILGKIVVVGTY"
     gene            complement(1480..2598)
                     /locus_tag="ACZPD3_06795"
     CDS             complement(1480..2598)
                     /locus_tag="ACZPD3_06795"
                     /inference="COORDINATES: protein motif:HMM:NF025826.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HIR1415739.1"
                     /translation="MGLFDKKICDICGEKIGLLGNRKLDDGNLCKDCAKKLSPWFEER
                     RHSTVEDIKRQLEYREKNKKAVMDFCITRQINTRNYNVFIDDNKGNFTVARKLDVNEN
                     PDIVPLSAIVQCRVDVDQQQHEETYTKDGENVSYQPPVYKYEFDYTMRIKVRTQWFDD
                     MDFRLNTFSISSDNRRELMEVEQTAYQIIAALTPNAAGMQSGMSGMNMTGGMQSGMPG
                     MNMNGGMQSGMPGMNMNGGMQSGMPGMNMNAGMQHGMSGMNMNAGMQPGMTGMNMNGG
                     MQSGMPGMNMNVGMQHGMPGMNMNGGMQSGMPGMNMNGGMQPGMPGMNMNGGMQQTGM
                     SETNMTGGMQQNNSSWKCQCGAENTGKFCEYCGQPRPF"
     gene            complement(2666..>3483)
                     /locus_tag="ACZPD3_06800"
     CDS             complement(2666..>3483)
                     /locus_tag="ACZPD3_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415740.1"
                     /translation="LWVAGTVGVLMTGLMSVNAYALEEKDVIGTWYVNELSMGGDILF
                     HPGVMGMEVTVDIKEDGKMETTSSVYGEEPEVDESEWKIENDKILMTHNDQTGECEYK
                     DGKITLTADGTTMILSQEKEEYEPYVPGKPVENPTMKDFEGEWSCTLMEVFGMQMPVN
                     ADFTGFEMSMSVEDGKAEIILIESGEETKVELQGDVEGNALVLKAVTEDEAGTMFFSL
                     DNMKFNLLDDGKLCLIAEDDAEESDDGEEADDSETGEVDFSVKTYFEKIIVTE"
BASE COUNT          905 a          945 c          523 g         1110 t
ORIGIN      
        1 aaatacgtac caacaactac aattttaccg agaatccgat catattgttc ccttgattct
       61 gtccgttcca gctcatccat aaactgtgac agcaattcca gctgacctgc tgtctgattc
      121 tgtatcatat ttaacagacg gttctgttct tctgtctttc tccgctcagc ctctttctga
      181 taagctttct gtaacagacg gtttctggca gtcagttcct cctgctgttc ccgaatatcc
      241 tgatactgat ccagcagtac cgaaatatcc tctgaccaaa acaaatgtcc tccccttata
      301 ggaatatggt tgatccggat tccctgttcc tgtacggatg atctgtctat tatgatttta
      361 cgcagttcag gcgacaactc aggaaagtca ccggaatgat aatagcgctg aaaagaatga
      421 tccgtaatct ctgcatccag tcctccggaa gtctggaata attcaaaata tcgattattg
      481 gtctgtatca atccacataa tatacaaccc tgaaaaattg ctgccattaa aaggcagcac
      541 actgccgtca tatcccctgc aatgattttg atgaaaggca ggcggagtat atttccgata
      601 ttccatccca gaagcaatat tcccggaatc accggcagcc agaactgttt ctttcccgga
      661 attctgcttt ttctgatcag aataatttcc atccctgtca gacagatcaa aatccatccc
      721 tgaatcacca taaaaacaat gccatagctg taatcttcat ccgaaaatgg gggctgtttg
      781 gaaaaacgaa ataccagctg atgcaaatca tttgtaaaaa cacttacgat caatatcgct
      841 gccacggtca ataaaattat aacctttctg accgttttct cttcctcctt ttcgcccaaa
      901 aacattgctg ctgccaaacc cagaaccgga atcaggagca tgggaatata atatagatac
      961 cagcagatat gttctccaag aggatcatga aaaatatgaa attttactgt ccgcacaaag
     1021 aaccagaata ccatcagaca gccaattgca gtcagacatc tgcgcgtttt tttatgtact
     1081 acatgctttc ccagataaat cacccaggca cagtacattc ccagataaat acagctacga
     1141 cactgatctg ccagatttct cataaaaaca ttttccgttc tcacctgcct gcagaaataa
     1201 gccagcaaca cacttatcag aatcactcca tatgacagag tcctcttttt actgtatgtc
     1261 atgaaagttc ctctcttacc ggagcgggtt tgaaaaatgc tttacgcaag atgtgcgtca
     1321 caatttgcag gaatgatttt tcaaacacac ccaacctatt tttgtaataa ttatactata
     1381 aagtactatt ttatcatata gttttcagaa gaatcatcta ttttcagagt ttttatcaca
     1441 aacagagtga acaatcctgt ccactctgtt tcccattgct taaaacggtc ttggctgacc
     1501 acagtattca caaaactttc cggtattctc tgcaccgcac tggcatttcc acgaactgtt
     1561 attctgttgc attcctccag tcatgttcgt ttctgacatt cccgtctgct gcattcctcc
     1621 gttcatgttc attccgggca ttccgggctg cattcctccg ttcatgttca ttcccggcat
     1681 cccagactgc atccctccat tcatattcat tccgggcatc ccgtgctgca ttcccacatt
     1741 catgttcatc ccgggcatcc cagactgcat ccctccgttc atgttcatcc cggtcattcc
     1801 aggctgcatt cccgcattca tgttcattcc tgacatcccg tgctgcattc ccgcattcat
     1861 attcatcccg ggcatcccag actgcattcc tccgttcatg ttcattcccg gcatcccaga
     1921 ctgcattcct ccattcatgt tcattcccgg cattccggac tgcattcctc cggtcatgtt
     1981 cattcctgac attccggact gcatcccggc tgcatttggt gtcagtgccg caataatctg
     2041 ataagcagtc tgctccactt ccatcaattc ccttctgtta tcactgctta tagagaaagt
     2101 attcaagcga aaatccatat catcaaacca ctgggttctc actttaatac gcatagtata
     2161 atcaaattca tatttatata ccggcggctg ataacttaca ttttctccat cctttgtata
     2221 tgtttcctca tgctgctgtt gatctacatc aactctgcac tgtacaatgg ctgataaagg
     2281 aactatgtcc gggttttcat tgacatccag tttccgcgca acagtaaaat tccccttatt
     2341 atcatcaata aaaacattgt aatttctggt attgatctga cgggttatac aaaagtccat
     2401 taccgctttt ttgttttttt cacgatattc cagctggcgt ttgatatcct ctaccgtact
     2461 gtgccgccgc tcctcaaacc atggtgataa ttttttcgca caatccttac acagatttcc
     2521 gtcatccagt tttcgatttc ccaataaccc aattttctct ccgcagatgt cacagatttt
     2581 cttatcaaat aatcccatac cctatcctcc ttttttatct gaaaacccat actgcctcac
     2641 cggaatggtg cggcagtatt ttaccttatt ctgtaacaat tattttttca aaatatgtct
     2701 ttacggaaaa atcaacttca cctgtttcag aatcatctgc ttcttcgccg tcatcagatt
     2761 cctctgcatc gtcttctgct atcaaacaca gttttccatc atccagtaaa ttgaacttca
     2821 tattatcaag actgaaaaac atagttcctg cctcatcctc tgttacagcc tttaatacca
     2881 gtgcgtttcc ttccacgtcg ccctgaagtt ctacttttgt ctcttctccg ctctctatta
     2941 agataatttc agcctttccg tcttccacag acatactcat ttcaaatcct gtaaagtcag
     3001 cattaactgg catctgcatt ccaaatactt ccatcagagt acagctccat tctccttcaa
     3061 aatccttcat ggttggattt tctaccggtt tgcccggcac ataaggctca tactcttcct
     3121 tttcctgact caggatcatt gtcgttccat ctgctgttaa ggttatctta ccgtccttgt
     3181 actcacattc tcctgtctga tcattatgag tcatcaaaat tttatcattt tctattttcc
     3241 actctgattc atctacttca ggctcttccc cataaacaga acttgttgtt tccattttac
     3301 cgtcttcttt aatgtccacg gtcacttcca ttcccattac tcctggatga aacaaaatgt
     3361 ctccacccat ggaaagctca tttacatacc aggttccaat aacatccttt tcttcaagtg
     3421 cataagcatt cacgctcatc aggcctgtca ttaagactcc aactgttcca gccacccaca
     3481 gtc
//
LOCUS       DBMDXG010000223         3466 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6177_length_3466_cov_1.456464, whole genome shotgun sequence.
ACCESSION   DBMDXG010000223 DBMDXG010000000
VERSION     DBMDXG010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6177_length_3466_cov_1.456464"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="ACZPD3_06805"
     CDS             <1..1262
                     /locus_tag="ACZPD3_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415741.1"
                     /translation="KYFKTNLEEAADQQAANIENDLEELTPDDSDADIPTDNFASAYS
                     DSLSLTVSMMSDLSAAAPEASVVETLLDKYGSMLFDNVTEGESSQETLTAGDISQDCT
                     VYEGQISAEDAVKTATAILEEAKSDSDIENILDTWTEKLSSNEDLHESFTKAVEDGLD
                     FLKDADTGDSDDSHLNTRIWVDETGRIAGRKIEFQEGDKITPVLNWQMTRDGSDFGYL
                     LSIETDDSGTLSLSGSGQIDGGKLNGTYKISQDDTAAAVIEVKDYDTESAKEGYLNGN
                     YTITFPADSSEDTDSSLSMLENFALVLDLNSAKDSGSVALSVESAGSTLGSFTVTSGA
                     GESVEIPDLTALGDVYDVTNEDDMSAYAATLDLTTLMDNLSNAGVPDEVITYVLSGGS
                     NSDAEDVTDENASEAESDAASAEAGAA"
     gene            complement(1286..2623)
                     /locus_tag="ACZPD3_06810"
     CDS             complement(1286..2623)
                     /locus_tag="ACZPD3_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415742.1"
                     /translation="MNNTFMKKKPILPLLASMAMPMVLSMLVNSLYNIVDSFFVAKIS
                     EQAMTALSLVFPVQNFINAVAIGFGVGINAQISFQLGAKNIKNANIAATHGMLFNIIH
                     GIIFTVICIPFMPVFLSMFTKAQEVISSGVQYSTIAFAFSTVIMISLSFEKIFQAVGR
                     MKLTMISLMIGCISNIILDPLLIFGVGIFPEMGIRGAALATGLGQVFTVIVYLIAYKK
                     CSIPVEISRKYLELNRNLDGKLYMVGIPAILNIALPSVLISFLNQILSAFSGSYVVVL
                     GIYYKLQTFLYLPASGIVQGMRPLIGYNYGAGEIKRVKKLFGISVLLNGMIMLAGTII
                     CFTASETLMGMFTENPETVRLGTAALQIISIGFIPSAISVTASGALEGLSKGAQSLVI
                     SLLRYIIVIIPAAYLLCYFAGADAVWNAFWICEFITAVVVGIGIKYYWNRLLQ"
     gene            complement(2774..>3466)
                     /locus_tag="ACZPD3_06815"
     CDS             complement(2774..>3466)
                     /locus_tag="ACZPD3_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415743.1"
                     /translation="QAEEHDESVLNPEIKNESDLSKKERRLIEKEKLKGMGPKKKLEY
                     IWMYYKPAIFGVIAVIALIFGIKDYYEQSKIKTVLSMTVVNSMANDTETPEQKIKETL
                     GYKDDPYSKVEIGVNLTTDSEMAEFDYNAQMAYVAQIQAGSIDIMVMPEKLYQTLKKN
                     EPFADLKELMGEEAFEKFGMQTDTTHISITDSELEQELGVIYDPVCIAVPYSAPNQEN
                     AVKWIKSLDSRK"
BASE COUNT         1019 a          808 c          659 g          980 t
ORIGIN      
        1 ataaatattt caagaccaat ctggaagaag ccgcagatca acaggctgcc aatattgaaa
       61 atgatcttga agaattaacc cctgatgatt cagatgcaga tattccgaca gacaatttcg
      121 cttccgctta ttctgacagt ctcagtctga ctgtttccat gatgtctgat ctgtcagcag
      181 cagctccaga agcttctgta gtagaaacac ttctggataa atacggatct atgctttttg
      241 acaatgtaac agaaggcgaa agcagtcagg aaactctgac agcaggtgat atcagtcagg
      301 actgtacagt atatgaaggc cagatttcag ccgaagacgc tgtaaaaact gcaactgcaa
      361 tcctggaaga agcaaaatca gacagtgata ttgagaacat cctcgatacc tggacagaga
      421 aactctcaag taacgaggac ctgcatgaaa gctttaccaa agctgtagaa gacggtcttg
      481 attttctgaa agatgcagat accggtgatt ccgatgattc tcatctcaac accagaatct
      541 gggttgatga gaccggcaga attgcaggac gtaaaattga gtttcaggaa ggtgataaaa
      601 taactcctgt tctgaactgg cagatgacca gggacggctc agactttggc tatcttctct
      661 ccattgaaac agatgattcc ggcacactct ccctgtccgg atccggtcag attgatggcg
      721 gtaaattaaa cggaacctat aaaatttctc aggatgatac agccgcagct gttatcgaag
      781 taaaggacta cgacaccgaa tctgcaaaag aaggttactt aaacggtaac tatactatca
      841 ccttccctgc tgattcttca gaagatacag attcttctct ttccatgctc gagaactttg
      901 cacttgtatt agatttaaat tcagcaaaag attctggttc tgttgcactg tccgttgaaa
      961 gtgcaggaag cactctcgga agcttcactg ttacttccgg tgcaggcgaa tctgttgaaa
     1021 ttccggacct tacggctctg ggagatgttt atgacgtaac caatgaagat gatatgagtg
     1081 cctacgcagc tacactcgac ctcactacac ttatggataa tctgtccaat gcaggcgttc
     1141 cggacgaagt catcacttat gttctcagcg gcggaagcaa ttccgatgcc gaagatgtta
     1201 ctgatgaaaa tgcctcagaa gcagagtctg atgctgcgtc tgctgaagca ggcgcagcct
     1261 gacttagcag ctttatcact atcgtctact gcagcagacg gttccagtaa tactttatcc
     1321 cgatacctac tactactgct gttataaatt cacagatcca gaacgcattc cacacagcat
     1381 ctgccccggc aaaatagcac agcagataag ctgccggaat gatcactata atatatctta
     1441 ataacgaaat caccagggac tgagcacctt tgctcagtcc ttctaatgca ccggaagctg
     1501 taacagagat ggcagatggg ataaacccga tactgataat ctgtaatgct gcagtgccaa
     1561 gtcttacagt ttccgggttc tcagtaaaca ttcccatcaa tgtctctgat gctgtaaagc
     1621 agatgatcgt tcctgccagc atgatcatac cattcagaag cacagaaatc ccaaaaagtt
     1681 tctttaccct ctttatttct cctgctccgt aattatatcc tatcaacggg cgcattccct
     1741 ggacaatgcc gcttgcaggc agatataaaa aagtctgaag cttataatag attcccagaa
     1801 ccactacata gcttccggaa aaagccgata atatctgatt caggaaagag atcagaactg
     1861 acggcagggc aatattcagg attgcgggaa tccctaccat atacagcttc ccgtccaggt
     1921 tcctgttaag ttccagatat ttcctgctga tctccaccgg aatactacac ttcttatatg
     1981 cgatcagata tacaataact gtaaatacct ggccaagacc agttgccagt gctgcacctc
     2041 tgatccccat ttccggaaaa atccccactc cgaaaatcag cagcggatcc aggataatat
     2101 tggaaataca accgatcatc aggctgatca ttgtcagttt cattctgccc acagcctgaa
     2161 aaatcttttc gaaggacagg ctgatcatga tcactgtgga aaatgcaaat gcaatagttg
     2221 aatactgcac accagatgag atcacttcct gcgcttttgt aaacatactg agaaatacag
     2281 gcataaacgg aatacagatt actgtaaaaa taattccatg gataatatta aagagcatac
     2341 catgggtagc tgctatattc gcatttttaa tattttttgc tcccagctga aaagaaatct
     2401 gtgcattgat tcccacaccg aaaccaatgg caactgcatt aataaagttc tgtacaggaa
     2461 ataccagaga cagagctgtc atcgcctgct cactgatctt tgccacaaaa aagctgtcta
     2521 cgatgttgta cagtgaattc accagcatag acagtaccat aggcattgcc atagatgcca
     2581 gaagcggcag aatcggtttt ttcttcataa aagtattgtt cattcttttt tcctttctct
     2641 gtttcattgc aacaaaaaac gccacacaat tatctctaca ttgctgtatg ataatcgtgc
     2701 ggcgaaaaga gttcatgttt gtcacaagac tgaaaaatcc acaccagttt tggtgtgatt
     2761 aacatatcat aatttatttc ctgctgtcaa gactttttat ccattttact gcgttttcct
     2821 gattcggtgc tgaatacggc actgcaatgc atacaggatc gtagattaca cctaattcct
     2881 gttcaagctc actgtcagta atggaaatgt gcgtagtatc tgtctgcatt ccaaacttct
     2941 caaaagcttc ctcacccatc aattccttca aatctgcaaa tggctcattc ttcttcaaag
     3001 tctgataaag cttctccggc ataaccataa tatcaatgga acctgcctgg atctgtgcca
     3061 cataagccat ctgtgcatta taatcaaatt ctgccatttc agagtccgta gtaagattca
     3121 cgcctatctc tactttacta tagggatcat ctttgtaccc aagtgtttct ttgattttct
     3181 gctcaggagt ttccgtatca tttgccattg aatttaccac tgtcatggac agaactgttt
     3241 ttattttgga ctgttcatag taatccttta ttccgaatat aagagcaatc actgcgatca
     3301 ctccgaaaat agccggttta taatacatcc agatatattc cagctttttc tttggcccca
     3361 ttccctttaa tttttctttc tcaatcagac gtctttcctt ctttgacaaa tccgattcat
     3421 tcttaatctc cggattcagc acgctttcat cgtgctcttc tgcctg
//
LOCUS       DBMDXG010000224         3445 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6234_length_3445_cov_1.688995, whole genome shotgun sequence.
ACCESSION   DBMDXG010000224 DBMDXG010000000
VERSION     DBMDXG010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6234_length_3445_cov_1.688995"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(86..796)
                     /gene="sigE"
                     /locus_tag="ACZPD3_06820"
     CDS             complement(86..796)
                     /gene="sigE"
                     /locus_tag="ACZPD3_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HIR1415744.1"
                     /translation="MSCYPLQVVSRMVMNRSGEVFYIGGNDVLPAPLEPKREAFILQK
                     LGTGQEEEAKSVLIEHNLRLVVYIAKKFDNTGVGVEDLISIGTIGLIKAINTFNPVKN
                     IKLATYASRCIENEILMYLRRNSKTKMEVSIDEPLNVDWDGNELLLSDILGTDEDVIS
                     RRLEDEVEISLLSKAIRKLSPREQTIIRLRFGLGKENAAEKTQKEVADLLGISQSYIS
                     RLEKRIMKRLKKEIVKYE"
     gene            complement(814..1671)
                     /locus_tag="ACZPD3_06825"
     CDS             complement(814..1671)
                     /locus_tag="ACZPD3_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR1415745.1"
                     /translation="MHYEIYIDVVFFTNLLIDYILIRFTGILFRCGRSRKRALLGAAA
                     GAVFSCWIIYLQSFLESGIFLPALVLLHGGCAAGMLVIGCNLKRGSLLLKAILTLYFA
                     AFLCGGFWEVIVSDKLTVKMFLILAAATWSGITALSYLMDSMRIRTKNIYPITIYYKG
                     CGYPFQGFYDSGNLLMDSVNGKPVSVGTEKVLSEICSEETVSCLKHLKENPGESGKPG
                     TAGLHPHFTFFHSIGKEQGMMLAVTFERLCIQTPAEVVCIDNPVFAFSFETSAFGKEY
                     EVLLNSRLL"
     gene            1967..2839
                     /locus_tag="ACZPD3_06830"
     CDS             1967..2839
                     /locus_tag="ACZPD3_06830"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR1415746.1"
                     /translation="MNNITGHTGLTALLGSPVAHSISPLMHNESFRLLGLDYVYLCFD
                     VNEETLPAAVAGLKTCGIRGFNLTMPNKNKIVELLDELSPEAQLIGAVNTVLNDNGKL
                     IGYNTDGYGFMQSVRDAGHDITGKNITVMGVGGASMAICAQSALDGAASVHIFARRTS
                     RYWNRTQKFAENLSAKTGCQVCLFDNDDHTALRDSIAQSYLLINATSVGMVPDIDDTI
                     IKDTSLFHPELVVADVVYEPQETRLLREAKAAGCQTFNGMYMLLHQGAKAFQIWTGQE
                     MPVSVIKEKYFSVK"
     gene            complement(2855..3334)
                     /locus_tag="ACZPD3_06835"
     CDS             complement(2855..3334)
                     /locus_tag="ACZPD3_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR1415747.1"
                     /translation="MRKHPNLEYIIVLGAHVEGTRLTKALLERTRRALQYMEENPETK
                     AVLSGGKGDGESITEAQAMCNYLVEHGIDRERLILEEKSTSTTENLKFSLGMIGLNHS
                     VGIVTNNFHVFRGTAIGKKCGCREIYPIPSRYRSWRLLIYIPREILAIIKDKLMGNM"
     gene            complement(3327..>3445)
                     /locus_tag="ACZPD3_06840"
     CDS             complement(3327..>3445)
                     /locus_tag="ACZPD3_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR1415748.1"
                     /translation="YLEQKKSGTVVKAKKIGRNDPCPCGSGKKYKYCCGKNA"
BASE COUNT          958 a          812 c          633 g         1042 t
ORIGIN      
        1 aaccgttcag tgctttcaca gttacaggcg gattgctgaa tagttactat tctttatatc
       61 attatactac attccccaat tacgtctatt catatttcac aatttccttt ttcagtctct
      121 tcatgattct tttttccagc ctagaaatat atgattgaga aattcccaga aggtctgcaa
      181 cctctttctg tgttttctct gcagcattct cctttcccag accaaatcta aggcggatga
      241 tcgtctgctc acgcggtgaa agctttctaa ttgcctttga taacaacgaa atctccactt
      301 catcttccag acgtcttgaa atcacatcct catcagttcc cagaatatca gaaagcagga
      361 gttcatttcc atcccagtct acgttcagcg gttcgtctat ggacacttcc atctttgtct
      421 tactgttgcg gcgaagatac atcaggatct cattctctat acatctggat gcataagtgg
      481 caagcttgat gttttttaca ggattaaagg tattgatcgc cttgatcagc ccaatcgttc
      541 cgattgaaat cagatcctcc accccgactc ctgtgttatc aaacttcttt gcaatataca
      601 ccacaagcct taaattatgt tcgatcagaa cagacttcgc ttcttcttcc tgccctgttc
      661 caagcttctg cagtataaac gcctcacgct tcggctccag tggtgccggc agcacatcat
      721 ttccaccaat ataaaaaact tctcctgaac ggttcatcac cattcttgac actacctgta
      781 atggataaca gctcatagtc acaacccctc ccgttaaagt aatctggaat tcaaaagtac
      841 ctcgtattcc ttaccaaaag cggatgtttc aaatgaaaat gcaaataccg gattgtctat
      901 gcagacaacc tctgctggag tctgaatgca gagtctctca aaagtaacag ccagcatcat
      961 cccctgttct tttcctatac tgtgaaaaaa agtaaaatgt ggatgaagcc cggcagttcc
     1021 cggtttccca gactctccgg gattttcttt caggtgtttt aaacagctta cggtttcttc
     1081 tgaacatatt tcagataaaa ctttttctgt ccctacagat acaggttttc cgtttacaga
     1141 atccataagc agatttccgg aatcataaaa tccttgaaaa ggatatccac atcctttata
     1201 gtatatggtt atcggataga tattctttgt ccgtattctc atggaatcca tcagatatga
     1261 caacgctgta atgccggacc acgttgctgc tgcaaggatc agaaacattt tgactgtcaa
     1321 cttatcactg acgatcactt cccagaaccc tccacacaga aatgcggcaa aatacagtgt
     1381 aagtattgct ttaagcagca gacttcccct tttcagatta catccgatca caagcattcc
     1441 tgcagcacag ccaccatgca gcaggacaag tgccggaaga aaaatcccag actccagaaa
     1501 agattgcaga taaatgatcc agcaggaaaa caccgcacct gcagcagctc ccagaagagc
     1561 tctttttctg cttcttccac atcggaataa tattcctgta aatctgatca atatgtaatc
     1621 tatcaggaga tttgtgaaaa aaactacatc tatgtatatt tcgtaatgca tttccttccc
     1681 tccgtctgat actgcctctg acttctcctt cacttcggta agtgagattc attatacgtg
     1741 ataaagtatg cggattttgt caaaacggag aaattatgtc ttattatttg tcgcactaaa
     1801 tcgacagttt ttatcatata aaccaaaatg aacatgtatt gacatataat tgaagttaga
     1861 ataaaatgat agataatttc tatgttttag aagggcatct tctatactgt cacaattcag
     1921 acagtatttt atgtctctgc tacaaaacag actggaggat tttaacatga ataatattac
     1981 cggacacaca ggtcttaccg cgcttttagg aagcccggtg gcacacagca tttccccttt
     2041 aatgcataat gaatcatttc gccttctcgg gcttgactat gtatatttat gttttgatgt
     2101 aaatgaagaa actcttcctg cagcagttgc aggattaaag acttgcggca ttcgcggttt
     2161 caaccttacc atgccgaata agaataaaat tgtagaatta ttggatgagc tttcccctga
     2221 agcccaactg atcggtgctg tcaatactgt actgaacgat aacggaaagt taatcggata
     2281 caatacagac gggtacggtt ttatgcaatc tgtacgtgat gcgggacatg atattaccgg
     2341 taaaaatata actgtcatgg gagtaggagg agcttcgatg gccatctgtg cacagtctgc
     2401 cctggacggt gcggcttccg tacatatttt tgcccgccgc accagcagat actggaatcg
     2461 cacacagaaa ttcgctgaga atctctctgc taaaacaggt tgtcaggtct gcctcttcga
     2521 caatgatgat catactgcat tgcgagattc cattgcacag agttatctgc tgatcaatgc
     2581 aacctctgta ggcatggttc cggatatcga cgataccatc atcaaagata catctctgtt
     2641 ccatcctgag cttgtagtgg cagatgttgt ctatgagcca caggaaaccc gactgctcag
     2701 agaagccaaa gcagccggat gccagacctt taatggcatg tatatgcttc ttcatcaggg
     2761 ggctaaagct ttccaaatct ggaccggaca ggaaatgccg gtttctgtta ttaaggaaaa
     2821 atatttctct gtcaaatagt gattcgcata acagtcacat atttcccatc agcttatctt
     2881 tgatgattgc caggatttct cttggaatat atatcagaag tctccatgaa cggtatctgg
     2941 aaggaatggg ataaatttct ctgcatccac atttctttcc tatggccgtt ccccggaaaa
     3001 catgaaaatt attggtgaca atgccaaccg aatgattcag tccgatcatt cccagactga
     3061 acttcagatt ctctgttgtg ctggtagatt tctcttccag aatcagtcgt tctctgtcaa
     3121 ttccatgttc cacaagataa ttgcacattg cctgagcttc tgtgatagat tctccgtccc
     3181 cttttcctcc ggacagaaca gccttagtct caggattttc ttccatatac tgtaaagccc
     3241 tcctggtacg ttccagaagg gcttttgtaa gccgggtgcc ttctacatgg gcaccaagca
     3301 cgataatata ttccagattt ggatgcttac gcattcttac cgcagcaata tttatatttc
     3361 ttgccgcttc cgcaaggaca aggatcatta cgtccgattt tctttgcttt gacaactgtt
     3421 cctgatttct tctgctcaag ataaa
//
LOCUS       DBMDXG010000225         3435 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6251_length_3435_cov_1.540492, whole genome shotgun sequence.
ACCESSION   DBMDXG010000225 DBMDXG010000000
VERSION     DBMDXG010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6251_length_3435_cov_1.540492"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..79
                     /locus_tag="ACZPD3_06845"
     CDS             <1..79
                     /locus_tag="ACZPD3_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1967 domain-containing protein"
                     /protein_id="HIR1415749.1"
                     /translation="EELEKAGIQEGDTVRMYGFDFDYYK"
     gene            153..449
                     /gene="yhbY"
                     /locus_tag="ACZPD3_06850"
     CDS             153..449
                     /gene="yhbY"
                     /locus_tag="ACZPD3_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIR1415750.1"
                     /translation="MTSKQRAYLKGLAMTMDPILQLGKGGLTPENTAAVDEALAAREL
                     IKISVLQNCLEDPRQMAEVLAERTRSQIVQVIGKKIVLYREGKNEKKKIELPRK"
     gene            463..1098
                     /gene="nadD"
                     /locus_tag="ACZPD3_06855"
     CDS             463..1098
                     /gene="nadD"
                     /locus_tag="ACZPD3_06855"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR1415751.1"
                     /translation="MADIKHRIGIMGGTFDPIHMGHLILGEKAYEQFRLEKVLFMPSG
                     NPPHKRNRQGRATDEERVEMVRRAITGNPHFELSLTEMHENGYTYTYHTLEMLKEKNP
                     DTDYYFIIGADSLYDFDTWREPERICRNCILVTAVRNHSTIAELEAEMNRLSLKYNGT
                     FLTLNTTNLDVSSEMLRNWIREDKSVRYYIPDPVIEYIRENQIYSLAEKKG"
     gene            1106..1696
                     /gene="yqeK"
                     /locus_tag="ACZPD3_06860"
     CDS             1106..1696
                     /gene="yqeK"
                     /locus_tag="ACZPD3_06860"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIR1415752.1"
                     /translation="MAEYDFIKMQKKLAKYLDEDRYEHTLGVMFTCAALAMVHDCDLI
                     TAQTAGLLHDCAKCIPNKKKLKMCSQHHISVSEFEQEHPFLLHAKLGAYVAKAKYDVT
                     DENILSAITWHTTGKPEMTLLEKIVYIADYIEPKRDKAPNLAIVRKLAFQDLDECMYK
                     ILGDTLAYLEENPKDIDNATKDAFLYYRDLHMKRQS"
     gene            1869..2228
                     /gene="rsfS"
                     /locus_tag="ACZPD3_06865"
     CDS             1869..2228
                     /gene="rsfS"
                     /locus_tag="ACZPD3_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR1415753.1"
                     /translation="MNRELEMAKLACRALDEKKGKDIKVIDIHEVSVIADYFVIASAS
                     NQNQVQAMVDNVDETLGRAGFEAKQIEGTRNSSWVLMDYGDMIVHVFDEENRLFYDLE
                     RIWRDGKVLDVNEFLEK"
     gene            complement(2638..3258)
                     /gene="lexA"
                     /locus_tag="ACZPD3_06870"
     CDS             complement(2638..3258)
                     /gene="lexA"
                     /locus_tag="ACZPD3_06870"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR1415754.1"
                     /translation="MPYGRITPKQQEILDYIKNEILNRGFPPAVREICEAVNLKSTSS
                     VHSHLEALEKNGYIRRDATKPRAIEIIDDNFNLVRREVVNVPIIGTVAAGQPLLAVEN
                     IEGYFPIPAEFMPNSQSFLLKVKGESMINAGIFDGDQVLVKQQSTAEDGDIVVALIDD
                     GATVKTFHKEKGYYRLQPENDTMEPILVHEGLQILGKVFGVFRLYE"
BASE COUNT         1075 a          648 c          736 g          976 t
ORIGIN      
        1 ggaagaactt gagaaggcag gcattcagga aggtgatact gtgcgtatgt acggatttga
       61 ttttgattac tataaataga ttcaataatg aaacagatgt ggtaagacgg atgattttgt
      121 ccgtttgcag agaataacag aggaaaataa aaatgacaag taaacagaga gcttatttaa
      181 aaggtcttgc aatgaccatg gatccaatcc ttcagcttgg aaagggtggt cttactcctg
      241 agaacacagc cgcagtagat gaggcgcttg cagccagaga actgatcaag atcagtgttc
      301 ttcagaactg cctggaggat ccgcgccaga tggctgaggt tctggcagaa cgtacacgtt
      361 cccagatcgt acaggttatc ggcaagaaaa ttgttttata ccgtgaaggc aaaaacgaaa
      421 agaaaaaaat tgaattaccc agaaaataat acggagtttt ttatggctga catcaaacac
      481 agaattggta ttatgggcgg tacttttgat cccatccaca tggggcatct gattctggga
      541 gagaaagcat acgaacagtt ccgactggaa aaggtactgt tcatgccttc cggaaatccg
      601 cctcacaaga gaaaccgtca gggacgtgcc acagatgaag aaagagttga aatggtccgc
      661 agggcgatta ccggaaatcc tcactttgaa ctgtcgctga ctgaaatgca tgaaaatggt
      721 tatacttaca cttatcatac actggaaatg ctaaaagaga agaatccgga tactgattat
      781 tattttataa tcggcgcaga ctccctgtat gactttgata catggcgtga accggaaaga
      841 atctgcagaa actgcattct ggtaactgct gtaagaaatc attccacaat cgcagagctc
      901 gaagcagaaa tgaaccgtct ttcgcttaaa tataatggca catttctgac actgaatacc
      961 acaaaccttg atgtatccag cgaaatgctc agaaactgga tcagagaaga taaaagtgtg
     1021 cgttactata ttcctgatcc ggtgattgag tatatcagag aaaaccagat ttacagtctg
     1081 gccgaaaaga aaggatgatc ttccgatggc agagtatgat tttataaaaa tgcagaaaaa
     1141 attagcaaaa tatctggatg aggatcgtta tgaacatacg ttgggagtta tgtttacatg
     1201 tgcagcactt gccatggttc atgattgtga tctgatcaca gcccagactg ccggactgct
     1261 ccatgactgt gcaaaatgta ttccgaacaa aaagaagctg aaaatgtgca gccagcatca
     1321 tatttcggta tctgaattcg aacaggagca tcctttttta cttcatgcca agcttggagc
     1381 ttacgtagct aaggctaagt atgatgttac agatgaaaat attctttctg ccatcacctg
     1441 gcatactacc ggaaagccgg agatgacact gttggaaaaa atcgtctata ttgccgatta
     1501 tattgaacca aaacgtgata aagctcctaa tcttgcaatt gtacgtaaac tggcttttca
     1561 ggatcttgac gaatgtatgt ataagatttt gggtgataca ctggcttatc tggaggaaaa
     1621 tccgaaagat attgataatg ctacgaaaga tgctttcttg tattacaggg atttacacat
     1681 gaaaagacag tcctgagtat aaattgtctc gactggcaga gttttacata taggggacag
     1741 cacaacaggg agaacagatt acggatataa atatttattt agagaagata tatgtagcta
     1801 ttcaatagtc tgttgatcat gaagatattg aacagttaca aaagcagata gaaagagagg
     1861 ttgtttccat gaacagagaa cttgaaatgg caaaacttgc ctgccgcgca cttgatgaga
     1921 aaaaaggaaa agatattaag gtaatagata tccatgaagt atctgtaatt gccgattatt
     1981 ttgtaattgc aagtgcttcc aaccagaatc aggttcaggc tatggttgac aatgtagatg
     2041 aaacacttgg cagagccgga tttgaagcaa aacagattga gggaaccaga aactccagct
     2101 gggttcttat ggattacggc gatatgatcg ttcacgtatt tgacgaagag aatcgtcttt
     2161 tctatgacct cgaaagaatc tggagagatg gtaaggttct tgacgttaat gaatttcttg
     2221 agaaataagt agaaaaaatt gttactgttc actctgttca cagcaacatg cttgcgatgc
     2281 acagacttta tgctaatcgc ataaagtcgc gcggtatgtc tcgggttttc tgtgaccttc
     2341 gttcacaaaa aacacctcgc gggatattac cagtgaacag taacgaaaaa tttacagtta
     2401 ttcggtgtag aactgcgtag cgaggcggat gattttatcc gcctgaattt gtgaaatctg
     2461 caaaataaaa tacagaggaa atcagatatc atcattaaga gatacctgat ttcctctgtt
     2521 gtttttttga attgatatag tataatttgc agtgtatggg gacttgcttg attcgattat
     2581 gagcaagttg cgaagcggat tgcgaatatc tgcctgacta ttcacgtatc agttatatta
     2641 ctcatacaaa cggaacacac caaatacttt gccaagaatc tgaagacctt catgtacaag
     2701 gattggctcc attgtatcat tttccggctg gagacgatag tatccttttt ctttatggaa
     2761 agtcttaact gtagcaccgt catcgatcag cgcaacaaca atatctccat cctctgctgt
     2821 ggactgctgt tttaccagaa cctgatctcc gtcaaaaatt ccggcgttta tcatactttc
     2881 cccttttact ttcaacagaa agctctgtga attaggcata aactctgcag ggatagggaa
     2941 atagccttca atattctcaa ctgccagaag tggctgtccg gctgccacag ttcctatgat
     3001 gggaacattc acaacctctc tgcggaccag attaaaattg tcatcaataa tctcaatcgc
     3061 acgtggtttc gttgcatctc tgcggatata tccatttttc tccagtgctt ccagatgtga
     3121 atgaacggaa gaagtagatt tcaggttgac tgcctcgcag atttcacgaa ctgccggcgg
     3181 aaatccccgg ttcaaaatct cattctttat gtaatcaaga atctcctgct gctttggcgt
     3241 gattcttcca tatggcataa ttataccctc ctttttaagt tattttttgt tcgtacactc
     3301 ttcataacca ttcagcagtc tgctattcta tgagatactg aacagtttct gattttttta
     3361 tcatatcata attcctgaca aaaagcaaac ttatattctg aaaatgtgtt cttacttatg
     3421 tgctcccgtt actgt
//
LOCUS       DBMDXG010000226         3433 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6257_length_3433_cov_1.732893, whole genome shotgun sequence.
ACCESSION   DBMDXG010000226 DBMDXG010000000
VERSION     DBMDXG010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6257_length_3433_cov_1.732893"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /gene="yqeB"
                     /locus_tag="ACZPD3_06875"
     CDS             <1..734
                     /gene="yqeB"
                     /locus_tag="ACZPD3_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR1415755.1"
                     /translation="PTAIRRMVSFCEAVYDGQSSVEGVLCRKVDSVEECEAVWKAGEI
                     PLMADTEGTVLKKYRPAALIDAILAKKNLGTTREMADLTVGLGPGFVAGEDVDYVVET
                     MRGHNLARIITKGAAMPNTGVPGIIGGFGKERVLHAPAAGEIHCISKIADIVEKDQVL
                     AWIGDTPVRASLTGVLRGMIRDGFTVPKGMKIADIDPRKEQKKNCFTISDKARCIAGS
                     VLEILLSEGVMPYEAPGRFSGTAAD"
     gene            694..1410
                     /gene="yqeC"
                     /locus_tag="ACZPD3_06880"
     CDS             694..1410
                     /gene="yqeC"
                     /locus_tag="ACZPD3_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR1415756.1"
                     /translation="MKHQVDFLELLQIDYEKYPVIAVVGGGGKTSLIYRLTDELIDKG
                     KRVIITTTTHMAGESELPFARGGDAVRVKELLDKERYVIAAEYEEDTGKYASLTDEKL
                     EELRELCDVMLVEADGAKHHPVKVPEKWEPVIPRCADIVISVIGLDCLGQPISQSAYR
                     MERTSEFLRKSLEAPITEEDIVKIATSICGLFKDVEERVYRVYLNKSDILKEKEPAEH
                     IVEELERKNTVAAYGSLLEE"
     gene            1417..2103
                     /locus_tag="ACZPD3_06885"
     CDS             1417..2103
                     /locus_tag="ACZPD3_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024214.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR1415757.1"
                     /translation="MKIAMIMLAAGNSRRFGANKLLYEIDGIPMYRHVLEQLDDTKKK
                     IENIYSEYSDITEDNNNDNNSDIVQLNNLYRNNITAKIICNIIVITQYDAIAEAVKTK
                     EIQVLYNPHPEDGISSSVKIGLNASLDADAVLFTVSDQPWLTSETICELIHVFLNTSK
                     GIACVSCQGKMGNPCIFDRKYYNELLSLEGDKGGKKVIMKHLDDTQIYEIEAGRELED
                     IDYYESIAVH"
     gene            2297..2572
                     /locus_tag="ACZPD3_06890"
     CDS             2297..2572
                     /locus_tag="ACZPD3_06890"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415758.1"
                     /translation="MSKYTEKWGVQIAGMIFQTLWSTHHKKSSGRGHRNFFCAIISLY
                     GRYPEYVAVIYISIKQLIYNKISAEQIKDGFWFRFFPGYRLWLSEVK"
     gene            complement(2508..3338)
                     /gene="arcC"
                     /locus_tag="ACZPD3_06895"
                     /pseudo
     CDS             complement(2508..3338)
                     /gene="arcC"
                     /locus_tag="ACZPD3_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - L-arginine deiminase pathway
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
BASE COUNT         1011 a          676 c          844 g          902 t
ORIGIN      
        1 aacccactgc gatcaggcga atggtttctt tttgcgaggc tgtttacgac gggcagtctt
       61 ctgtggaagg agttctctgc agaaaagttg acagtgtaga ggaatgcgag gcagtctgga
      121 aagccgggga gattcctctt atggcagaca cagaaggaac tgtccttaag aaatacagac
      181 ctgcagcact gatagatgca attcttgcaa aaaagaatct tggaaccacc agagagatgg
      241 cagatctaac cgttggtctt ggaccgggat ttgtggcagg tgaggatgtg gattatgtag
      301 tggagactat gagaggccac aatcttgcca gaatcatcac aaaaggcgct gctatgccaa
      361 acacaggagt tccgggtatc attggaggat ttggaaaaga gcgcgtgctc catgctcctg
      421 cggcagggga aatccattgt atctcaaaga ttgcagatat cgtggagaag gatcaggtcc
      481 ttgcctggat cggagataca cctgtcaggg cttctcttac aggagttttg cgtggcatga
      541 tcagagatgg atttacagtg ccgaagggga tgaaaattgc agatattgat ccgcgcaaag
      601 aacaaaagaa aaactgtttc acgatttccg acaaagcccg ctgtattgcc ggcagtgtat
      661 tggaaatcct gctttcagaa ggagtgatgc catatgaagc accaggtcga ttttctggaa
      721 ctgctgcaga ttgattatga aaaataccct gtgatcgctg tagttggcgg cggcggcaaa
      781 accagcctga tctaccgcct tacagatgaa ctgatcgata agggaaagag agtgatcatt
      841 accacgacca cccatatggc aggagaatct gaacttccgt ttgccagggg cggagatgca
      901 gtcagggtaa aagaacttct ggacaaagaa agatatgtga ttgctgcgga atatgaggag
      961 gacacaggta aatatgcgtc tctgacagat gaaaaactgg aagaactgag agaattatgc
     1021 gatgtaatgc ttgtggaagc ggacggagca aagcatcatc cggtaaaagt cccggagaaa
     1081 tgggagcctg taatccccag atgtgcggac attgtgatca gtgtgatcgg gctggactgt
     1141 ctggggcagc caatcagtca gagtgcttac cgtatggaac gtacctcgga atttctcagg
     1201 aaaagtctag aagctccgat cactgaagaa gatattgtaa agattgcgac ttctatctgc
     1261 ggacttttta aggatgtgga agaaagagta tacagagtgt atctgaacaa gtcagatata
     1321 ctcaaagaga aagaacctgc agagcacatt gttgaggaac tggaaaggaa gaatacagta
     1381 gcggcttacg gaagcctttt ggaggaataa attattttga aaattgcaat gatcatgctg
     1441 gcggctggaa acagccgccg ttttggggca aataaactgt tatatgaaat agacggaata
     1501 cctatgtatc gacatgtgct ggaacaactg gatgatacaa agaagaaaat agaaaatatc
     1561 tattcggaat attcagatat tactgaggat aataataatg ataataattc agatattgtt
     1621 cagttaaata acctgtacag aaataatatc acagcaaaaa taatctgtaa tattattgtt
     1681 attactcaat atgatgccat tgcagaagca gtgaaaacaa aggaaataca ggttttgtat
     1741 aatccccatc cggaagatgg aatttcttcc tctgtaaaaa tcggtctgaa tgccagcctg
     1801 gatgcagatg cagtgctttt tactgtgtct gatcagccct ggcttaccag tgagacaatc
     1861 tgtgaactga tccatgtgtt tttaaacact tccaaaggaa tcgcctgtgt ttcctgtcag
     1921 gggaaaatgg gaaatccatg tatttttgac aggaaatatt acaatgagct gctttctctg
     1981 gaaggagata aaggcggtaa aaaagtgatc atgaaacatc tggatgatac acagatttat
     2041 gagatagaag caggcaggga actggaagat attgattatt atgaatctat agcagtccac
     2101 tagtacatcg tttcgtaaat aactatacca atcgcgtagg atgcggattc gagtgtgctg
     2161 gtataaaaac gcaggcgtag cgggctacgc tgaggctttt agacctgtgc aatcggattc
     2221 gcagacaagt gagtggtata gttatttcga aaacgatgta ctaaagtgtg tctgaaaaat
     2281 gccttccgca agatgtatgt caaaatatac cgaaaagtgg ggcgtgcaga ttgcaggaat
     2341 gattttccag acactctggt ccactcatca caaaaaaagt tccgggcgag gtcaccggaa
     2401 ctttttttgc gcaattattt cgctatatgg cagatatcct gaatatgtgg cagttatata
     2461 tatcagtatt aaacagctga tatataataa gatatctgct gaacagatta aagatggatt
     2521 ttggttccgg tttttccctg gataccgtct ttggctttct gaagtaaagt gatcatggca
     2581 gttcttcctt ctttggagct tgcaaaatca acggcagcct gaactttcgg gagcatggaa
     2641 cctggggcaa actgtccctc attaatatat ttcttagcgt catctacagt gatgtcatcc
     2701 agccattctt cgttttcctt gccgaagttt acagcaacct tctccactgc tgtaagaatg
     2761 ataagcatat ctgcatccag ctctttggcc aggagacagc ttgcataatc cttatcaata
     2821 actgctgatg cacctttgag atgatgtccg ttaagaacaa cgggaattcc gccgccaccg
     2881 cagcagataa cgattttatt tgcatccaca agactgttga tcgcatcctg ttcaacgatt
     2941 tcaacaggct ttggagatgc aactacacga cgataaccac gccctgcgtc ttctttcatg
     3001 atatgtccat atgccttggc ctgatggtct gcttcctctt ttgtcaggaa tttgccgatc
     3061 ggcttggatg gattttcaaa agcaggatca ttgggatcta cacgaacctg agtgacaaca
     3121 gtgacgacag gtgtgcggac aaatcctctg attcgaagtt cctctctcaa agcattctgc
     3181 agatcatatc cgatgtaagc ctgactcatg gcaacacaga cagagagagg agtgtttggc
     3241 tgattctcat cttctctgga gagagcagac atggcattgt tgatcatgcc aacctgtggc
     3301 ccgtttccat gtacaacaac aacctgatga ccttcttgtc tgaccgataa tgcctacacc
     3361 gtgagttgtt ttccagccct gtgttgctct ctggtcgagt gggtcagatg taccgcctga
     3421 gccaaagccc tta
//
LOCUS       DBMDXG010000227         3430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6262_length_3430_cov_1.736257, whole genome shotgun sequence.
ACCESSION   DBMDXG010000227 DBMDXG010000000
VERSION     DBMDXG010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6262_length_3430_cov_1.736257"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="ACZPD3_06900"
     CDS             <1..713
                     /locus_tag="ACZPD3_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HIR1415759.1"
                     /translation="KFGTAKLVNKHGKYYLHIPVTYEVEESNISDICNVVGIDRGINF
                     VVATYDSKQKSGFVSGKAIKQKRANYSRLRKELQMRHTPSSRRRLKAIGQRENRWMQD
                     INHQVSKALATGNPKHTLFVLEDLTGIRNVTERVKTKNRYVSVSWSFYDLEQKLIYKA
                     KQNQSSVIKVDPRYTSQCCPACGHTEKSNRNKKIHLFTCKNCGYTSNDDRIGAMNLYR
                     MGINYLADSQVPNTVVTE"
     gene            1143..2225
                     /gene="yedE"
                     /locus_tag="ACZPD3_06905"
     CDS             1143..2225
                     /gene="yedE"
                     /locus_tag="ACZPD3_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HIR1415760.1"
                     /translation="MKNEKGTIVLAGGVIGLIAAILVFFGNPANMGFCIACFLRDTTG
                     ALGLHSAAAVQYIRPEIIGLVLGACIISLAKKEFRPRGGSAPVTRFTLGAFVMIGCLM
                     FLGCPFRMILRLAGGDGNALFGLVGFVAGILTGTIFLKKGYTLKRSYKMPKLEGSIYP
                     AFQIVMLLLLAAAPAFIHFTEPEGGPGAKHAAIIIALAAGIIVGILAQRTRLCMVGGI
                     RDAVLFKEYKLLFGFAAILVTALVMNLILGAVTGTSYFNPGFAGQPIAHTDGLWNALG
                     MYLAGFGCILLGGCPLRQLILAGEGNTDSAVTVLGLMAGAAFAHNFGLASSGEGPTAN
                     GKIAVIIGIVVVAVIAAVNSMRKEEA"
     gene            2317..2523
                     /locus_tag="ACZPD3_06910"
     CDS             2317..2523
                     /locus_tag="ACZPD3_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR1415761.1"
                     /translation="MKKVDARGLSCPEPVIRAKNAMESGDKEYEILVDNVVAKENVSR
                     FATHQGYQVQATEQGDDILLKLTR"
     gene            2520..2762
                     /locus_tag="ACZPD3_06915"
     CDS             2520..2762
                     /locus_tag="ACZPD3_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HIR1415762.1"
                     /translation="MITYIATFYSHYGAIQFRRNCQAMNLSAEVMPVPRDLSSSCGTC
                     VRFHTEADFPEKTEEVEQIVRVEPQGYVGIYHADEE"
     gene            complement(2835..3095)
                     /locus_tag="ACZPD3_06920"
     CDS             complement(2835..3095)
                     /locus_tag="ACZPD3_06920"
                     /inference="COORDINATES: protein motif:HMM:NF016114.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR1415763.1"
                     /translation="MLKQENVSGDIALRFFDHDGQECNTTLKERMISISMEQFKKIDR
                     KISLVSGVSKANAVLSALKGGLVDVLILDSNLAEALLKLTQE"
     gene            complement(3089..3418)
                     /locus_tag="ACZPD3_06925"
     CDS             complement(3089..3418)
                     /locus_tag="ACZPD3_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016114.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR1415764.1"
                     /translation="MRNPRRLKESDILGVTWGSTVYHMINYLNPSQKVNAKFVTLHGS
                     IACCENELDVRTLVSRMARAFFGENYYLLTDALMSSKEASRIMKEEKKNPFLHLRILC
                     SQRIWRC"
BASE COUNT         1014 a          671 c          797 g          948 t
ORIGIN      
        1 acaagtttgg tactgccaaa cttgtaaata aacatggtaa atactatctg catataccag
       61 ttacctatga ggttgaagaa agtaatattt ctgatatttg taatgttgta ggtattgaca
      121 gaggtattaa ctttgttgtt gcaacttatg acagtaaaca gaagtctgga tttgttagtg
      181 gtaaagctat taaacagaaa cgggctaatt attccaggct tcgtaaagaa cttcaaatgc
      241 gacatacgcc atcctcaagg cgaagactaa aagctatcgg tcagcgagaa aaccgttgga
      301 tgcaggatat taaccatcag gtatcgaagg cactcgccac cggtaatcca aagcatactc
      361 tctttgtatt agaagatttg actggtattc gcaatgttac agaacgtgtc aaaacaaaaa
      421 accgctatgt ttctgtatca tggtcttttt atgaccttga gcagaagctg atttacaaag
      481 cgaagcagaa tcaatcttct gtaataaagg tggatcctcg ttataccagt caatgctgtc
      541 ctgcttgtgg acatactgaa aagtctaatc gtaataagaa aatacatttg tttacttgca
      601 aaaactgcgg ttatacgtct aatgatgacc gtattggagc tatgaatctg tatcgtatgg
      661 gaataaacta tcttgctgat agccaagtac ctaatacagt tgtaacagag taaactctgc
      721 tacaaagggt gctgtcaacc acccaatgat gtaacgccac tttaggcagc aatgcctatt
      781 ggggataaaa ggtcggaggc gtaagccgtt actacgactg ggcagttaca agcccattac
      841 ctttaggtga tgggtagttg acagtaacaa atcccccgat ttttatataa ttaaagatta
      901 tatgatatga gaattgcagg agctagtttg ctgcggaaca attcgtagat atcaggttag
      961 gggcaaaata ctttttgccc ctaacatcat tatagtatag cacaacttat aaataatttc
     1021 tataaataac tataaaatac ggctataatg aattagtgat ttcagcataa aaatgataaa
     1081 atagatacaa agaaattgtg ctgacaacaa aaaaagtcag tgcaaaagga ggaaagaaat
     1141 aaatgaaaaa cgaaaaggga accatagttc tggcaggagg cgtaatcggt ctgatcgctg
     1201 caattctggt gttttttgga aatccggcaa atatgggctt ctgtattgcc tgtttcctga
     1261 gagataccac aggtgcgctg ggacttcatt cagcggcagc agttcagtat atccgcccgg
     1321 agatcattgg tctggtgctt ggagcatgta tcatttcact tgctaagaaa gagtttcgtc
     1381 caagaggcgg ctctgcacct gtgaccagat ttacacttgg cgcatttgtt atgatcggat
     1441 gtctgatgtt tcttggatgt cctttccgta tgattctgcg tctggcaggc ggagacggaa
     1501 atgctctgtt tggacttgtt ggatttgtag ccggtattct tacaggaact attttcctga
     1561 agaaaggtta cacactgaaa cgttcttata agatgccgaa gctggaaggc agtatttatc
     1621 cggcattcca gatcgtgatg ctgctgttac tggcagctgc acctgcattt atccatttca
     1681 cagaaccgga aggtggaccc ggagcaaaac atgctgcaat cattattgca ctggcagcag
     1741 gaattattgt tggtatcctg gctcagagaa cacgtctgtg tatggtcggc ggtatccgtg
     1801 atgcggtact tttcaaagaa tataaattac tgttcggttt tgctgcaatt cttgtgacag
     1861 cactggtaat gaatctgatc ctcggtgcag ttacaggaac atcctatttc aatccgggat
     1921 ttgcgggaca gccgatcgca cacacagatg gactgtggaa cgcactggga atgtatctgg
     1981 caggctttgg atgtatcctt cttggcggct gtccgctgcg tcagcttatc ctggcaggag
     2041 agggaaacac agattcagct gtgactgttc tcggtctgat ggcaggcgct gcttttgccc
     2101 ataacttcgg actggcatct tccggtgaag gtcctacagc aaacggaaag attgcagtta
     2161 ttattgggat tgtggtagtt gcagtaatcg cagcagtgaa ctccatgaga aaagaagaag
     2221 cctgattgtc ggaatgcaga catcatttcc gcaatcaata cgctcacttt gcggaaagta
     2281 tttttttaga cacactctaa aaggagagag agacgaatga agaaagtaga tgcaagaggt
     2341 ttatcctgtc cggaaccagt gatccgtgca aagaatgcaa tggaatccgg agacaaagaa
     2401 tatgagatcc tggttgataa cgtagttgca aaagagaatg tatccagatt tgcaacacat
     2461 cagggatatc aggtacaggc aacagagcag ggagatgata tcctgctgaa acttacaaga
     2521 tgatcacata tatagcaacc ttttattccc attatggagc tattcagttt cgaagaaact
     2581 gccaggcaat gaatctgagt gcagaagtca tgcctgtccc aagagattta agctcctcct
     2641 gcggaacctg tgtgagattt catacagaag cggacttccc ggagaaaaca gaggaagtcg
     2701 agcagatcgt cagagtagaa ccgcagggat atgttgggat ttatcatgcg gatgaagaat
     2761 aagagaaccc ttctgtggac aaattgtcgg cagaagggtt cttgtttatt ttatagagat
     2821 gcaggcataa aatattactc ctgtgtaagt ttcagcagtg cttcagcaag attggagtcg
     2881 aggatcagca catccaccag cccgcccttt aaagcgctta acacagcatt tgctttggac
     2941 acacctgata caagggaaat cttgcggtca atttttttga actgctccat gctgatcgat
     3001 atcatgcgtt ctttaagtgt agtattacat tcctgtccgt catgatcaaa gaagcgaagg
     3061 gcaatatctc cggaaacatt ttcctgtttc agcatctcca gatcctctgt gaacataaaa
     3121 tccggagatg caagaacgga ttcttcttct cttccttcat gatccgggag gcttctttgc
     3181 tgctcataag agcgtctgtg agaagataat agttctctcc gaagaatgcc cttgccatgc
     3241 gggataccag agtacgcaca tccagttcat tttcacagca ggcaatactg ccatgaagag
     3301 taacgaactt cgcatttacc ttctgggaag gattgagata attgatcata tggtaaacag
     3361 tactgcccca ggtaacgccc agaatatcac tttcttttaa cctacgggga tttcgtattc
     3421 aggcatatga
//
LOCUS       DBMDXG010000228         3429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6265_length_3429_cov_1.475361, whole genome shotgun sequence.
ACCESSION   DBMDXG010000228 DBMDXG010000000
VERSION     DBMDXG010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6265_length_3429_cov_1.475361"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="ACZPD3_06930"
     CDS             <1..1180
                     /locus_tag="ACZPD3_06930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR1415765.1"
                     /translation="GIEHFKEINDNFGQEYGDFVLRKTADCISGCLSEGQKVYKLVAD
                     EFLILDVSSDQVRDAAKLYDKVRAATDRFIESNEFKVMYTVSGGIVSFAALEGNQYSE
                     ALKLTDFALNEAKTLGRNRCYIFDGETYRKFLRKREITQELREAVLNGCQGFAAFYQP
                     VFAEDKKVPYGAEALMRFTSEKLGIISPAEFIPILEETGLIIPAGRWMMREAMGKCSE
                     IRKVLPGFRVSINISQVQASKSDVIQDISAEMKRAGLSLEALIVELTESDLLEQNINE
                     KHFLTELKRMGISLALDDFGTGYSNFHYLSELKPEIIKIDRSFTVKAVADEQEYYLLN
                     QFCTMIHNLDLRICIEGVENEQEWAMIRKLYPEFTQGYFWGKPCEYEEFMRKFTESRL
                     "
     gene            1306..2292
                     /locus_tag="ACZPD3_06935"
     CDS             1306..2292
                     /locus_tag="ACZPD3_06935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR1415766.1"
                     /translation="MKKKFFRLSATGLLFFSGVMEIGWRFFNMVVCCKRGGRKKERKK
                     WFELSHIRDNHPRNGYAKEYDESRAWCETQKMQDCYIQSVDGLKLHGFYLPAEHAKRF
                     VILSHGYRGSRFGSLSFMVKYLHEHQCNLLFMEQRCCGESEGKYITFGAKEKWDVQRW
                     AIYVSERNKEKLPIYLYGQSMGAAAVLMASGYRLPSEVKGLIADCGFQSMERQMRDMA
                     DNWFHLHYIPLLLKEMNCLCHFVAGFRMKDADTTEAMKRNTRPVLFFHGEKDTYVYPN
                     NSFQNYMLCKAPKELVIVQGARHLCSAYADPELYQRTVMEFFEKYDGSNSEK"
     gene            2592..>3429
                     /locus_tag="ACZPD3_06940"
     CDS             2592..>3429
                     /locus_tag="ACZPD3_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HIR1415767.1"
                     /translation="MTNYYPLTAAQKMHHNWIMDYGTQQVSGVSVVASVQAELDFGLL
                     KKCIQMETERSGCTRIRFTKPDKDGNVQQYLVKQDPRDIGFKDLSGMRSLAKADELMQ
                     QWAYETFDGDDIPMCEFTMLKLPEGYNGFFVHMDHRLIDSCGLVVMIGDLFQLYTYYK
                     YGTAYPQELADFETVLKKDLAKAGNGKRFAKDKKFWDDQLDALGEPLYSDIQGPSVLE
                     EARKRHGNPRLRSSDIEMKDLFVAVKDYYLEPGPTKNLIDFCMNHQLSMTNLLLLGIR
                     TYL"
BASE COUNT         1049 a          567 c          876 g          937 t
ORIGIN      
        1 tggaatcgaa cattttaagg aaataaatga taacttcgga caggagtacg gtgactttgt
       61 gcttaggaaa acggctgatt gtatttccgg atgcctgtca gaaggacaga aagtgtacaa
      121 gcttgtggca gatgaatttc tgattctgga tgtttcttct gaccaggtga gggatgcagc
      181 taaactttat gataaagtga gagctgcaac agacagattt attgaatcta acgagtttaa
      241 ggtcatgtat acggtttcag gaggaattgt gtcatttgcg gcattagagg gaaatcagta
      301 ttcagaagcc ctgaagctga cggattttgc tttgaatgag gcaaagacgc tgggaagaaa
      361 ccgctgttat atatttgacg gagaaaccta caggaaattt cttcggaaga gggagattac
      421 ccaggaactg cgggaggctg tgctcaatgg ctgccagggt tttgcggctt tttatcagcc
      481 tgtatttgct gaagataaaa aagtacctta tggagcagaa gctttaatgc gttttacttc
      541 tgaaaaactc gggataattt caccggcaga gtttattcca attctggaag aaaccggtct
      601 tattattccg gctggccgat ggatgatgag agaagctatg ggaaaatgca gcgagatccg
      661 gaaagtactt ccgggtttta gagtgagtat taatatttcc caggttcagg catccaagtc
      721 tgatgtaatc caggatatca gtgcggaaat gaaaagagca ggactgtcgc tggaagcgtt
      781 gatcgtggaa ttgacagaga gtgacctttt ggaacaaaat atcaatgaga agcattttct
      841 cacagaactg aagaggatgg gaatcagcct tgcattggat gactttggaa ccggatattc
      901 gaacttccat tatctcagtg agctgaaacc ggagatcatt aagattgacc gctctttcac
      961 tgtgaaagca gttgcagatg aacaggaata ttatctgctg aatcagttct gcaccatgat
     1021 ccataatctg gatctgagga tttgtatcga aggcgtggaa aatgagcagg aatgggccat
     1081 gatccgaaaa ctgtacccgg aattcacaca gggatatttc tgggggaagc cttgtgagta
     1141 tgaagaattt atgagaaaat ttacagaaag cagattatga gaaagctggc gtgaatattt
     1201 cgggctacac tgaggctttt tcatttgtac aacaggattc gcagacaagc aagtggtgta
     1261 gttatttcaa acacattctg acaggtaaga agaggagaaa atcagatgaa gaaaaaattt
     1321 ttcaggttgt cagcaacagg attgcttttt ttttcagggg ttatggagat tggctggcgc
     1381 ttttttaaca tggtggtctg ctgcaaaaga gggggaagga aaaaggaaag aaagaaatgg
     1441 tttgaattat cccatatacg ggataatcat cccagaaacg gatacgcaaa ggaatatgat
     1501 gagagcaggg catggtgcga gactcagaag atgcaggact gttatatcca aagcgtggat
     1561 ggattaaaac tccacggatt ctatctgccg gcagagcatg caaagcggtt tgttatctta
     1621 agccatggtt acaggggcag cagatttggt tccctgtcat ttatggtaaa atatctccat
     1681 gagcatcaat gtaatcttct gtttatggaa cagcgatgtt gtggagaaag tgaaggaaaa
     1741 tatattacct ttggagcaaa ggaaaaatgg gatgtgcagc gttgggcaat ttatgtgtcc
     1801 gagagaaata aagaaaagct tccaatctat ctgtatggac agtctatggg agcagccgct
     1861 gtactgatgg catccggata caggcttccg tcagaggtga aagggctgat cgcagactgt
     1921 ggttttcagt ctatggaaag acagatgcgg gatatggctg acaactggtt tcatttgcat
     1981 tatattcctt tgcttttgaa ggaaatgaac tgtctgtgcc attttgtggc aggatttcgt
     2041 atgaaagatg cagataccac agaagctatg aaaaggaata caagacctgt ccttttcttt
     2101 catggagaga aggatactta tgtgtacccg aacaattcat tccagaacta tatgctgtgc
     2161 aaagcaccaa aggaactggt cattgtacag ggagcaaggc atctgtgcag tgcctatgca
     2221 gatccggaac tgtatcagcg tacagtgatg gagttttttg agaaatatga tggcagtaat
     2281 tctgaaaaat aatgtgactg ttttcaaccg ccaaaggcga ttacgaatga caacaatctc
     2341 tgaccgggac ttatcagatg cgttttgtta aatgcatgat aagtctcggt caattttatg
     2401 taaagtgttc attaagtgct gagggtactg gcatttggga aaaatctgtg ctatttttgt
     2461 cacgtaataa aaaagtaatg ttgtagctgc cagtacatta tttttaaaat aactgtgggc
     2521 tactgaacaa gtatgtaatg tttttgtacc cgggaaaaga gaaaaaacgg gtgatggagg
     2581 ttaaataaag tatgacaaac tactatcctt taacagcagc acagaaaatg caccataact
     2641 ggatcatgga ttacggtaca cagcaggtat ccggtgtcag tgttgtggcg tctgtacagg
     2701 ctgaactgga ttttggatta ttaaaaaaat gtattcagat ggagacagaa cgttccggat
     2761 gcaccagaat tcgttttacg aagcctgata aagacggaaa tgtacagcag taccttgtaa
     2821 aacaggatcc aagggatatc ggattcaagg atctgtcagg aatgagaagc cttgcaaaag
     2881 cagacgaact gatgcagcag tgggcatacg aaacctttga cggtgatgac attcccatgt
     2941 gcgagtttac tatgctgaag ctgccggaag gatataatgg tttctttgta catatggatc
     3001 acaggctgat tgattcctgt ggactggtag taatgatcgg tgatttgttt cagttatata
     3061 cctattataa atatggaaca gcctatccgc aggaactggc agactttgag acagtactga
     3121 aaaaagacct tgcaaaagca ggcaacggga aacgttttgc aaaagataag aaattctggg
     3181 atgatcagct ggatgcactg ggcgaacctc tgtattctga tattcaggga ccatccgtac
     3241 tggaggaagc aagaaaacgt catggaaatc ccagacttcg ttcttctgat attgagatga
     3301 aggatctgtt tgtggcagtt aaggattatt atctggagcc gggaccaact aaaaatctga
     3361 ttgatttctg tatgaaccat cagctgtcta tgacaaacct tcttttgctt ggtatccgta
     3421 cttatctgt
//
LOCUS       DBMDXG010000229         3421 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6277_length_3421_cov_1.036446, whole genome shotgun sequence.
ACCESSION   DBMDXG010000229 DBMDXG010000000
VERSION     DBMDXG010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6277_length_3421_cov_1.036446"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="ACZPD3_06945"
     CDS             <1..883
                     /locus_tag="ACZPD3_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HIR1415768.1"
                     /translation="GLESNPGFKLTLPLVKKVAYGAVFGDILMKCVYRMRPYELEEGI
                     VNRKHKIWEQRVISFLSGSSISHSQFKKMCREMVHEFDTIPISDVKKPRVGIVGEILV
                     KFLPAANNHLAELLESEGAEAVVPDLIDFMCYCFYNQNFKVENLGFKKSKATMANWGI
                     KAIEWVRKPASEALAQSRHFAPPADIRNLAKMASPIVSTGNQTGEGWFLTGEMMELIH
                     GDVPNIVCIQPFGCLPNHIVGKGVIKEIRREYPTANIVAIDYDPGASEVNQLNRIKLM
                     LSTAQKNLKKAEEKMHK"
     assembly_gap    1065..1074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1129..2757)
                     /locus_tag="ACZPD3_06950"
     CDS             complement(1129..2757)
                     /locus_tag="ACZPD3_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative polysaccharide biosynthesis protein"
                     /protein_id="HIR1415769.1"
                     /translation="MNQKNNLVKNASFLMIAAMISKVIGLLYKSPLSNIIGSLGMGYT
                     SLAQNAYMILLMIASFSIPQAVSKLISERIALKDYRNAHKFFKGAMIYAMVIGGVVGL
                     FCLFGAGLIIPQNQKDAIPALQILAPTIFLSGILGVFRGYFQAYRNMMPTSISQIIEQ
                     VFNAAVSLLAAWGFINAFSDGTENSIAKWGAAGSTVGTGAGVVTALAFMLLVYGVNRR
                     KILHRVEKDHRHQEESYKEIFRVIILVVSPIILSAFVYNVNAYINGYLFSDILGRRGG
                     DAAQIGILYAEYATYFMTIINIPLTLSSTAPTSMIPEVSALYATGDIEATRECIDQTV
                     QLSMVVSAPCAMGLAVLAQPIVFLLYGNSTGLAANLLILGSFSILLNGMSNISNGVLQ
                     AIGQQRIPVITAAIALVVDIVVVVVLLFTTNLGVYALLIAMVIYSVVVCVLNDRAMKK
                     YLQYKNPWKEGYLYPVLASVPMGIVAGCICYGLNIFVKSNFICLIVSIPVAAVVYLFA
                     YLIISKPSESQLRRIPGGSYLIRIAEKLPFWQNN"
     gene            complement(2813..>3421)
                     /gene="ung"
                     /locus_tag="ACZPD3_06955"
     CDS             complement(2813..>3421)
                     /gene="ung"
                     /locus_tag="ACZPD3_06955"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006773885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR1415770.1"
                     /translation="KLYKTVMTEYQTRKIFPPADDLFNAFHFTPLNEVKVVILGQDPY
                     HNDGQAHGLCFSVKRGVETPPSLVNIYQELHDDCGCYIPNNGYLEKWAKQGVLLLNTV
                     LTVRAHQANSHRGIGWEQFTDAAIQVLNEQDRPIVFLLWGRPAQMKKSMLNNPKHLIL
                     EAPHPSPLSAYRGFFGCKHFSKTNEFLVANGLEPIDWQIENI"
BASE COUNT         1014 a          785 c          703 g          909 t
ORIGIN      
        1 cggtctggaa agcaatcccg gattcaaact tacgcttcca ttagtgaaga aagttgctta
       61 tggtgccgta ttcggagata tcctgatgaa atgcgtatat cgtatgcgtc cttatgaact
      121 ggaagaggga atcgtaaaca ggaaacataa aatctgggaa cagcgtgtga tctctttctt
      181 aagcggaagc agtatcagcc acagccagtt taagaaaatg tgccgcgaaa tggttcacga
      241 atttgatacc attccgatca gtgatgtgaa aaagcctcgt gtcggcatcg taggtgagat
      301 ccttgtcaag ttcctgcctg ctgcaaataa tcatctggca gagcttctgg aatctgaagg
      361 cgctgaggca gttgtaccgg atctgatcga tttcatgtgc tactgcttct ataatcagaa
      421 cttcaaggta gaaaatcttg gattcaagaa gtccaaggca accatggcaa actggggaat
      481 caaagcaatc gaatgggtga gaaagcctgc aagcgaagct cttgcacaga gccgccattt
      541 tgcaccgcct gctgatatca gaaatctggc aaaaatggca tctccgatcg tttccaccgg
      601 aaaccagaca ggtgagggat ggttcctgac aggtgagatg atggaactga tccatggaga
      661 tgttccgaat attgtttgta tccagccttt cggatgtctg ccgaaccaca ttgtaggtaa
      721 gggtgttatc aaagagatcc gcagagaata tccgacagcc aatattgtag ccatcgacta
      781 tgatccgggt gcaagtgaag ttaaccagct caaccgaatc aaactgatgc tctccacagc
      841 tcagaaaaac ctgaaaaaag cagaagaaaa aatgcataaa taataccgta atggatatat
      901 tgcaatgtat tttaccttgc aatatatccg ccttagagcg tatttgaaaa atcattcccg
      961 caatctacat atcctgcttt gcgaaaagca tttttcaaac gcgctcaaaa aagagttata
     1021 tttcagaaat tttaaatgtg tttctatttc agaaatacat ttaannnnnn nnnnatgaaa
     1081 tataactctt ttatttttta taacttctgt ttttataact tctgtttttt aattattctg
     1141 ccagaacgga agtttctctg caatccttat cagatagctt ccacccggaa ttcttctaag
     1201 ctgtgattct gacggcttgc tgatgatcag gtaagcaaac agataaacaa ccgcagcaac
     1261 aggaatggaa acgatcagac aaatgaaatt ggattttaca aaaatgttca gtccatagca
     1321 gatacagcct gcaacaattc ccatgggaac agatgcaaga acaggataca gatatccttc
     1381 tttccatgga ttcttatact gcagatattt cttcatggct ctgtcattta acacacatac
     1441 aaccacagaa tagatcacca ttgcgatcag cagtgcataa actccaaggt ttgtagtaaa
     1501 cagcagcact accactacca caatatccac aaccagtgcg attgcagccg taatcaccgg
     1561 aatcctctgc tgtccgattg cctgcagaac tccattggaa atatttgaca ttccattgag
     1621 caggatcgaa aaagaaccaa ggatcagcag gttggctgcc agtcctgtgg aatttccgta
     1681 aagcaaaaat acaatcggct gtgcaagcac tgccagtccc atggcacatg gggcagaaac
     1741 taccatagaa agctgtacgg tctggtcaat gcactctctg gtcgcttcaa tgtctccggt
     1801 ggcatacagg gcggaaacct ccggaatcat ggaagtaggt gccgtactgg acagcgtcag
     1861 aggaatatta ataattgtca taaagtaagt agcatactct gcatataaaa taccaatctg
     1921 tgccgcatct ccacctcttc ttcccagaat atccgagaaa agatacccat tgatataagc
     1981 atttacatta taaacaaagg cgctgagaat gatcggcgaa acaaccagga taatcacacg
     2041 aaaaatctct ttgtaggact cttcctgatg tctgtggtcc ttctctaccc ggtgcaggat
     2101 ttttctgcgg ttcacaccat ataccagaag catgaatgcc aatgcagtca ccactccggc
     2161 tccggttcct actgtggaac ctgctgcccc ccattttgca atgctgttct ctgttccatc
     2221 tgagaatgca ttgatgaatc cccaggctgc cagaagactg actgccgcat taaatacctg
     2281 ctcaatgatc tgggagatag atgttggcat catatttctg tatgcctgaa aatatcctct
     2341 gaatacccca agaataccgg acaaaaaaat cgtcggagcc aggatctgaa gtgccggaat
     2401 tgcatccttc tgattctgag ggatgatcag ccctgctcca aagagacaga acaaaccgac
     2461 cactccgcca atcaccatgg catagatcat ggcaccctta aagaacttat gtgcatttct
     2521 gtaatccttc agagcaatac gctcggagat cagcttggat accgcctgtg gaatactaaa
     2581 ggatgcgatc atcagaagga tcatataggc attctgtgca agacttgtat agcccattcc
     2641 aaggcttccg ataatattgg aaaggggact cttgtacaga agaccgatca ccttggagat
     2701 catagctgca atcatcagaa aggatgcatt ttttactaaa ttgtttttct gattcatgtt
     2761 atcactgtgt gtcttttacc tgactacaga ttacctcttt tcttatatat tcttatatat
     2821 tttctatctg ccagtcgatt ggttccagac cattggcaac aagaaattcg tttgttttac
     2881 tgaaatgttt acatccgaaa aatcccctgt aagcagacag aggacttgga tgaggtgcct
     2941 ccagaatcag atgcttcgga ttattcagca tggatttttt catctgcgcc ggacgtcccc
     3001 atagcagaaa tacaatagga cggtcctgct cattcagaac ctggatcgca gcatccgtaa
     3061 actgctccca gccgatcccc ctgtgtgaat ttgcctgatg agcgcgtaca gtcagaactg
     3121 tgttcagaag aagcactccc tgcttcgccc acttctccag atagccgtta ttggggatat
     3181 agcatccaca gtcatcatga agctcctgat aaatattcac cagggacggt ggcgtctcca
     3241 ctcctctctt tacggaaaaa caaagcccat gagcctgccc gtcattatga tagggatcct
     3301 gacccagaat cactaccttc acttcattca acggtgtaaa atgaaatgca ttaaacagat
     3361 catccgcagg aggaaaaatc ttcctggtct gatattctgt catcaccgtt ttatataatt
     3421 t
//
LOCUS       DBMDXG010000230         3417 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6284_length_3417_cov_1.242461, whole genome shotgun sequence.
ACCESSION   DBMDXG010000230 DBMDXG010000000
VERSION     DBMDXG010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6284_length_3417_cov_1.242461"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..3040
                     /locus_tag="ACZPD3_06960"
     CDS             209..3040
                     /locus_tag="ACZPD3_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR1415771.1"
                     /translation="MISSAKKYMAVLLVFVCVCMIFSPLTAYAAEDSSQHETVKVGFF
                     AMDGYHMMDEEGNRSGYGYDFLRLMARYWDVDYEYVGYDQSWDDMQQMLEDGEIDMVT
                     SARKTPDREEKFDFSRPIGTNYGMLTVRSDNSTIVDDNYSTYNGMRVALLNGNTRNEE
                     FADFADNKGFTYVPSYFDTTAEMEEALQSEKVDAIVTSSLRKTNNERIVDKFGSSDFY
                     VIVKKGNTELLNEINYAIDQMNAVEGDWKTTLYKKNYESIETKNLEDTEQEKSIIAQY
                     SKDNPLHVLCDPSRYPYSYNENGEMKGIIPDYFRKIADYAGISYEFLTPATRDEYIAY
                     QKNKEVTNISIDARLETDNYAETKKWGLTAPFITMQLARVTRRDFDGEINVVATVDQT
                     ASNSIADAMAPGAEKLMCSTRQEMMEAVRKGKADAAFVYYYMAQAFVNSDTTGTMTYT
                     LLEQPTFTYRMVVSSTENHALAGILTKAMYAMPQNLVEDLAARYTTYKAAELTFVDWI
                     RLHPVATVWVLLIFGWLLTTMAMIAMRLSARKKAQKAAQETAEEMAELAEHAQAANKA
                     KTAFLSHMSHDMRTPMNAIMGFTGIAMKNNPSDEVKNCLEKIDESSEHLLSLINDVLD
                     LTRVESGKVKYNPVPTDLKSITDFALDITKGFLTNRDISFKIQREEAKIPNVLVDSVR
                     LRDVLVNILSNAVKFTPDGETITFEARCQEKGGDGYINMHYRISDTGIGMSEEFTKEV
                     FEEFAQEDSDVRTQYHGAGLGMAIVKKYVDMMGGTISVQSKKHEGTTFTVDIPLEITD
                     KECNKSDIGFSEKVNLTGVKVLLAEDNELNAEITTVQLEEFGMNVERAVDGKNAVEIF
                     RNHPEGTFDVILMDIMMPEMNGYEAAKAIRAMNDRPDGKNIPIIAMTANSFAEDVQAS
                     LDAGMNAHLSKPIVIEEVIKTILRYVHND"
     gene            3088..>3417
                     /locus_tag="ACZPD3_06965"
     CDS             3088..>3417
                     /locus_tag="ACZPD3_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR1415772.1"
                     /translation="MTMQECYKAIGGNYEAVLGRLHSEALIQRFTLKFLEDQSYLQLK
                     QALENKNYEDAFRSAHTLKGVCQNLSFDRLYEVRIMKTHSELGAAIIKDMDFSQDYPL
                     VHTAWEIC"
BASE COUNT         1097 a          590 c          864 g          866 t
ORIGIN      
        1 atgactgcaa atgcatttag agaagatgca gaaaagtgta tggaagcagg aatgaacgca
       61 catcttgcaa agccattaga tgataaaaca atcaagcaga ctatatgcga agaattgaga
      121 agcagtaggg acaggtgaaa atttattaaa ccaggaggtt gacaaggaga cgtatagaga
      181 aatatatttg acagacagga gatttaatat gatttcgagt gcaaagaaat atatggcagt
      241 gttactggtt tttgtatgcg tgtgtatgat attttctccc ctgactgcat atgcggccga
      301 ggacagttcg cagcatgaaa ctgtcaaagt cggtttcttt gccatggatg gataccatat
      361 gatggatgaa gaggggaacc gcagcggtta tggctatgat ttccttcggc tgatggcccg
      421 ttattgggat gtagattacg aatatgtcgg atacgatcag agctgggatg atatgcagca
      481 gatgcttgaa gatggcgaga tcgacatggt aacatctgcc cgcaaaacac cggacagaga
      541 agaaaagttt gatttttcac gtccgatcgg aactaattat gggatgttga ccgtccgcag
      601 tgacaacagc acgattgtgg acgacaatta cagcacttac aacggtatgc gtgtggcgct
      661 tttgaacgga aacacccgga atgaagagtt tgcggatttt gcagacaaca aaggatttac
      721 atatgttcca tcttattttg atacgaccgc agagatggag gaagcccttc agagcgaaaa
      781 agttgatgcg attgttacca gttctctgag aaaaacaaac aatgagcgaa tcgtggataa
      841 atttggcagc agcgatttct atgtcattgt taaaaagggc aatacagaat tgctgaatga
      901 gatcaattat gcgatcgatc agatgaatgc agttgagggt gactggaaaa caacgcttta
      961 taagaaaaat tatgaaagca tagaaactaa gaatctggaa gatacggagc aagaaaaaag
     1021 tattattgca caatattcca aggacaaccc gcttcatgtt ctgtgtgatc cgtcccgtta
     1081 tccatattct tataatgaaa atggtgagat gaaggggatt atcccggatt acttccggaa
     1141 aattgcagac tatgccggga tttcctatga gtttcttaca cctgccacca gagatgagta
     1201 tattgcttat cagaaaaata aggaagtcac aaatataagt atcgatgcac gcctggaaac
     1261 agataactat gctgagacga aaaaatgggg gcttactgca cctttcatta caatgcaact
     1321 ggcaagggtg acacggcgtg actttgatgg agaaatcaat gttgttgcta cggttgacca
     1381 gacagcatca aattcaattg cagatgcaat ggcacccggt gcagaaaagc tgatgtgcag
     1441 tactcggcag gaaatgatgg aagctgtccg taagggtaaa gccgatgctg ccttcgtcta
     1501 ctattacatg gcacaggcgt ttgtgaatag tgacactacg ggcaccatga catatacgct
     1561 gctggaacag cctacattca cctaccgcat ggtagtttcg tcaacagaga accatgcgct
     1621 ggctggtatt ctgacgaaag caatgtatgc catgccccaa aatcttgtcg aagatctagc
     1681 agcacggtat accacataca aagcagcaga gctgacattt gttgactgga tccgtctgca
     1741 tcctgttgct actgtttggg ttcttcttat tttcggatgg ctgctgacta ctatggctat
     1801 gatagcaatg cgtctcagtg caagaaaaaa agctcagaag gcagcccagg agacggctga
     1861 ggaaatggcg gaactggcag agcatgcgca ggctgcaaac aaggcaaaaa cagcatttct
     1921 gtcacatatg tcccatgata tgagaacacc gatgaatgca atcatgggat tcaccggtat
     1981 tgcaatgaaa aataatccgt cagacgaagt gaaaaattgt ctggaaaaga ttgatgaaag
     2041 ttcagagcat ttactgtcgc tgatcaatga tgtattagat ctgactcgcg tggaaagtgg
     2101 aaaagtgaaa tacaatccgg taccgacaga tttgaaaagt atcacagatt ttgcactgga
     2161 tattacaaaa ggctttctta cgaacaggga tatcagcttt aagatccagc gtgaagaagc
     2221 aaagatcccc aatgtacttg tggattctgt acgtctgcga gatgtgctgg taaatattct
     2281 gagcaatgcc gtgaagttca ctccggatgg agagacgatt acatttgaag cccggtgtca
     2341 ggaaaagggc ggggatggat atataaatat gcattaccgc atttcggata ctggtattgg
     2401 catgagtgag gaatttacaa aagaagtttt tgaggaattt gcacaggaag attccgatgt
     2461 gagaacgcag taccatggag ctggactggg gatggctatc gtaaagaagt atgtagatat
     2521 gatgggtgga accatttccg tgcaaagcaa aaagcatgag ggaaccactt ttactgtgga
     2581 tattcctctg gaaattactg ataaggaatg taataagtca gacataggtt tttctgaaaa
     2641 ggtaaatctt acaggtgtga aagttcttct tgcagaggat aatgagctga atgcagaaat
     2701 tacgaccgtg cagctggaag agtttggaat gaatgtagaa agagctgtag atggaaagaa
     2761 tgctgttgag attttcagaa atcatccgga aggcacattt gatgtaatcc tgatggatat
     2821 catgatgcct gaaatgaacg gttatgaagc ggcaaaagca atcagggcga tgaatgacag
     2881 accggatgga aaaaacattc ctattatagc aatgacagcc aattcatttg ctgaagatgt
     2941 tcaggcatcg ttggatgcag gaatgaatgc acatttgtca aagccgattg tgattgagga
     3001 agttataaaa acgattttaa ggtatgttca taatgattag cagaggttca tgaattagtg
     3061 gaatacgatt gacaggaggg ctaaaagatg acaatgcagg agtgctataa agctatcgga
     3121 ggaaactatg aggctgtttt gggacgcctt cacagcgaag cactgattca gagatttaca
     3181 ttaaagttcc ttgaagacca gagttatcta cagttaaaac aggcattgga aaataaaaat
     3241 tatgaagatg ctttccgcag tgcgcacaca ttgaaagggg tgtgtcagaa tctttccttt
     3301 gaccgtctgt atgaggttag gattatgaaa acccatagtg agcttggcgc ggctataatt
     3361 aaggatatgg acttttcaca agattatcca ctggtacata ccgcatggga aatctgc
//
LOCUS       DBMDXG010000231         3412 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6293_length_3412_cov_2.607369, whole genome shotgun sequence.
ACCESSION   DBMDXG010000231 DBMDXG010000000
VERSION     DBMDXG010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_3412_cov_2.607369"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(113..1492)
                     /gene="murC"
                     /locus_tag="ACZPD3_06970"
     CDS             complement(113..1492)
                     /gene="murC"
                     /locus_tag="ACZPD3_06970"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR1415773.1"
                     /translation="MYQIDFHKPLHIHFIGIGGISMSGLAEILLGEDFVISGSDSKSS
                     PLTQALEKKGATIYYGQRATNITDDVDVVVYTAAIHPDNPEFACAKEKGLPMLTRAEL
                     LGQIMRNYDTPVAISGTHGKTTTTSMVSHILLAGDCDPTISVGGILPAIGGNIRVGNS
                     ETFVTEACEYTNSFLSFFPKISIILNMDADHLDFFKDIDDIRHSFRRFAELLPADGTL
                     IINADTPKYEDIIRDLPCNVITYGLEHDADYQAADITYDKYGHASFSVLRNGVKVGSY
                     YLKVPGIHNVSNALAAIALGHLLGLSEEVIIKGLGSFTGTDRRFQYKGEVAGVTIVDD
                     YAHHPTEIEATLHAAHNYPHKKLWCVFQPHTYTRTKALLPEFAKALSLADHVVVADIY
                     AARETDTLGISSEDLQKRIQELGTPCEYFPTFDEIENYLLANCQEGDLLITMGAGDVV
                     NIGEHLLGK"
     gene            1886..3160
                     /locus_tag="ACZPD3_06975"
     CDS             1886..3160
                     /locus_tag="ACZPD3_06975"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR1415774.1"
                     /translation="MIKKEMIAMLLAGGQGSRLGVLTEKVAKPAVAFGGKYRIIDFPL
                     SNCINSGIDTVGVLTQYQPLRLNTHIGIGIPWDLDRNEGGVTVLPPYEKSTSSEWYTG
                     TANAIFQNMDYMEQYNPDYVLILSGDHIYKMDYEVMLDFHKANKADVTIACMPVPIEE
                     ASRFGIMVTDDIGRITEFEEKPEHPSSNLASMGIYIFSWPALKEALMSLKDQNSCDFG
                     KHVLPYCKEKGERLFAYEYNGYWKDVGTLGSYWEANMELIDIIPEFNLYEEFWKIYTK
                     GDIIPPQYISADAVTDRCLIGEGAEIYGEIHNSVIGPNVVIGKGSVIRDSIIMRNSTI
                     GEGVQMDKAIIAEDVTIGNNVVLGCGEEAPNVLKPAVYAFGIATVGERSVIPDNVRIG
                     KNTAISGITVPEDYPDGELAGGQVITAKDGDE"
     gene            3160..>3412
                     /locus_tag="ACZPD3_06980"
     CDS             3160..>3412
                     /locus_tag="ACZPD3_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009219011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR1415775.1"
                     /translation="MRAIGIILAGGNNNRMRELSNKRAIAAMPIAGSYRSIDFALSNM
                     ASSHIQRVAVLTQYNARSLNEHLSSSKWWDFGRKQGGLFV"
BASE COUNT         1016 a          648 c          808 g          940 t
ORIGIN      
        1 aattattttc cgaattgtcc tgtgtaaatt gctgatatct atgtctggca gcggattgca
       61 aataactgtc tgacaactgt ctgttataat aggaagttat ccgtgagaaa tattatttgc
      121 ccagcagatg ttccccgata tttacaacat ctccggctcc catggtgatg agcagatctc
      181 cctcctggca gtttgcgaga agataattct caatctcatc aaaggtcggg aaatattcgc
      241 atggcgttcc aagttcctgg atacgcttct gcagatcctc tgaagaaatt ccaagagtat
      301 cggtttctct tgctgcataa atatccgcaa ctaccacatg atctgccagg ctgagtgctt
      361 tggcaaactc cggaagcaga gctttggtac gggtatatgt atgtggctgg aatacacacc
      421 ataatttctt atggggataa ttgtgtgcgg catgaagagt tgcttcaatt tctgtaggat
      481 gatgtgcata atcgtctacg atcgtaaccc ctgcaacttc tcctttatac tggaaacggc
      541 ggtctgttcc tgtgaaactt ccaagtccct tgatgatcac ttcttcggaa agaccaagaa
      601 gatgaccgag ggcaattgct gccagtgcat tggaaacatt gtggatgccc ggcactttca
      661 gataatagct gccaacctta acgccattgc gcagaacgga gaaggaagca tgtccatatt
      721 tgtcataagt gatatctgct gcctgataat ctgcatcatg ttccagacca taagtgatca
      781 cattacatgg aagatcccgg atgatatctt catatttcgg agtatctgca ttgatgatca
      841 gtgtaccgtc agcaggaaga agctctgcaa agcggcggaa ggaatgacgg atatcgtcaa
      901 tatccttaaa gaaatccaga tggtctgcat ccatattcag gataatactg atcttcggga
      961 aaaagctcag aaagctgttt gtgtattcgc aggcttctgt gacaaaagtc tcagaattgc
     1021 ctacgcggat gtttccgccg atagcaggaa gaatacctcc cacactgatg gtcgggtcac
     1081 agtctcctgc aagaagaatg tgagatacca tagatgttgt ggttgttttt ccatgagttc
     1141 cggaaatggc aacaggagta tcataattcc tcatgatctg tccgagaagc tctgcacgtg
     1201 tgagcattgg aagtcctttt tcctttgcac aggcaaattc cgggttgtca ggatggatag
     1261 ctgcggtata taccacaaca tctacgtcat cggtaatgtt tgtggcacgc tgtccgtaat
     1321 agatggtggc tccttttttc tccagtgcct gggtaagagg actggatttg gaatcggaac
     1381 cggaaatgac aaaatcctcg ccgagaagaa tctcggccag tccgctcatg ctgatgccgc
     1441 cgataccgat aaaatgtata tgaagtggtt tgtgaaaatc tatctgatac atagtatgtt
     1501 ctccttgatt aattatttac aaatctttat aaatccggta actgtaacat attcgcatag
     1561 ttactggaac tgttttatct gtctgtttat tatactccct gtgccttaaa atgaaaagga
     1621 cttcttttgg ttgataaaaa attacaaaaa acgacattgg atatataaaa aatgcctaat
     1681 aatatatcgg tggaaataca gactcaataa aaaacgtgtg caaaaatcat aaaaaaacaa
     1741 aaaatgagaa acaaaaattg taaataatgt tcactaacag ataaaagtgt gttataatta
     1801 aataatcata acctgtaact gttcagtgcc tcgcgaaaca gcagactgac gaatagttac
     1861 cataactaca acaagaggtg attgcatgat taagaaagaa atgatagcta tgcttctggc
     1921 cggcggtcag ggcagcagac tgggtgtact gacagaaaaa gtagcgaaac ctgcagttgc
     1981 ttttggcggg aaatatcgta tcatagattt tcctttaagt aactgtatta attcaggcat
     2041 cgatacagta ggtgtattga cacagtatca gccattgcga cttaatacac atatcggaat
     2101 tggtattcca tgggatcttg acagaaacga aggtggtgta acagtactgc cgccttacga
     2161 gaagagtacc agcagtgagt ggtacacagg aacagccaat gcaatcttcc agaatatgga
     2221 ttacatggaa cagtataatc cggattatgt cctgatctta tccggtgacc atatctataa
     2281 gatggattat gaagtaatgc ttgatttcca taaagcgaat aaagcggatg tgacgattgc
     2341 ctgtatgccg gtacctattg aggaagcaag ccgttttggt atcatggtaa cagatgacat
     2401 aggacgcatt acggagtttg aggagaaacc ggagcatcca agcagcaatc tggcgtccat
     2461 gggaatttat attttcagct ggcctgcttt aaaggaagca ctgatgtctc tgaaggatca
     2521 gaacagctgt gattttggca aacatgttct tccatactgc aaggagaaag gtgaaagact
     2581 tttcgcctac gaatacaatg gatactggaa ggatgtagga acactgggtt cttattggga
     2641 ggccaatatg gaactgatcg atattattcc ggaatttaat ctctatgaag aattctggaa
     2701 gatttacaca aagggagata ttatcccgcc tcagtatatt tcggcagatg cggttacaga
     2761 cagatgtctg atcggtgagg gtgcagaaat ttacggggaa atacataatt ctgtgatcgg
     2821 accaaatgtt gtgatcggaa aaggcagcgt gatccgtgat tccattatca tgcgcaattc
     2881 tactattggt gagggcgtac agatggataa ggctattatt gccgaggatg ttactattgg
     2941 aaacaatgtt gtactgggat gtggtgagga agcaccgaat gtgctgaaac ctgcagttta
     3001 tgcatttgga attgctacgg ttggtgaacg cagcgtgatc ccggataatg taaggatcgg
     3061 aaagaataca gcaatctcag gtataactgt tcctgaggat tatcctgatg gtgagctggc
     3121 cggcggacag gtaattacag caaaggacgg tgacgaataa tgagagcgat aggtattatt
     3181 ttggctggcg gtaataataa ccgcatgaga gaattatcaa ataagagggc aatcgcagca
     3241 atgccgattg ccggaagcta cagaagtatc gacttcgcac tgagcaacat ggcgagttct
     3301 catattcaga gagttgctgt tctgacacag tacaatgccc gttctctgaa tgaacattta
     3361 agctcttcca aatggtggga tttcggaaga aaacagggtg gtctctttgt at
//
LOCUS       DBMDXG010000232         3410 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6299_length_3410_cov_1.536114, whole genome shotgun sequence.
ACCESSION   DBMDXG010000232 DBMDXG010000000
VERSION     DBMDXG010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6299_length_3410_cov_1.536114"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(74..688)
                     /locus_tag="ACZPD3_06985"
     CDS             complement(74..688)
                     /locus_tag="ACZPD3_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR1415776.1"
                     /translation="MNTQNSTITKLAETALMAALCYVSFTFLQIKIPVPGGDATSIHI
                     GNAFCVLAALLIGGVYGGLSGAIGMGIADLMDPVYITGAPKTFVLKFCIGLITGLVAH
                     KIAKINESTDKKYVFKWSVIASVAGLAFNVVADPIVGYFYKQYILGQPQELAQALAKM
                     SAVATLFNAVVSVILVAVIYNAVRPVLIKSHLLTVTGKKKEQAA"
     gene            complement(1076..2248)
                     /locus_tag="ACZPD3_06990"
     CDS             complement(1076..2248)
                     /locus_tag="ACZPD3_06990"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR1415777.1"
                     /translation="MDYAKESLKMHYDLKGKIEVVSRAKVDSKDALSLAYTPGVAQPC
                     LEIQKDVNKSYDLTRRWNTVAVVTDGTAVLGLGDIGPEAGMPVMEGKCVLFKEFGGVD
                     AIPLCVRSKDVDEIVKTVSLLAGSFGGINLEDISAPRCFEIEKKLKECCDIPIFHDDQ
                     HGTAVITLAGVINALKLVGKKLDEVKIVTSGAGAAGIAIIKLLMSMGLKNVIMTDRKG
                     AIYEGREGLNPIKEEMAKITNFNKEKGTLAEVIKGADIFIGVSAPGTLTQDMVRTMAK
                     DPIIFACANPVPEIFPDEAKAAGAAVVSTGRSDYPNQVNNVLCFPGLFRGVLDARASD
                     VNDEMKVAAAYAIAGLVSDEELTAEYILPAAFDPRVKDAVAKAVAEAARKSGVARI"
     gene            complement(2447..>3410)
                     /locus_tag="ACZPD3_06995"
     CDS             complement(2447..>3410)
                     /locus_tag="ACZPD3_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415778.1"
                     /translation="YLDGKMLDDNTYAGVLRIPVEDMTVDDAGWTKFYEVRNAFFKEK
                     GIDVDSEDFSFDKLAQTLGMDEYSDENLPQVGGPAISDYVKDIKVPDRFAMELDLKDI
                     VGTLPDDQDTTPDIPQDLRDEYNQKMAEHGISTDDADYESLTEEQKDLEHQFFTEMWN
                     EYFERYPEAIEGNNRYNSWTLKGDWKFNVDVEKNTSDTVKKDVNVVDENGDGVLSITK
                     TPFEITMKMQDPEAKYFAVMLDANGDIMPYGGVSNSNNTYAIQDRDISTVYIYLCDYY
                     EYMDELKGYYWSDDYEEKAKTRTFKQLLDERAVAGTEVHFDTDK"
BASE COUNT          863 a          817 c          626 g         1104 t
ORIGIN      
        1 ctcataaata ctctatacac caaaaaagca tttcagtctg ctgtatgatg caaaactgaa
       61 atgcttttca tttttatgct gcctgttctt ttttcttacc ggtaactgta agcagatgac
      121 tcttaatcag taccggacgt actgcattgt agatcactgc taccaggatc acagaaacta
      181 ctgcattgaa taatgtggct acagcactca tttttgcaag tgcctgtgcc agttcctgag
      241 gctgtccgag aatatactgt ttgtagaaat atcctacgat cggatctgcc acaacattaa
      301 atgcaagacc tgctacagaa gcaatcacac tccatttgaa aacatatttc ttatctgtac
      361 tttcattgat ctttgcaatc ttatgtgcta ctagacctgt gataaggcca atacagaatt
      421 tcagtacaaa tgtctttggt gcgcctgtga tatatacagg atccatcagg tctgcgattc
      481 ccattccgat ggcaccggat aatccgccgt atacaccgcc gatcagaagt gctgccagta
      541 cgcagaatgc atttccgata tggatggatg ttgcatcacc gcccggtaca gggatcttaa
      601 tctgcaagaa tgtaaaagat acatagcata acgcagccat aagagctgtc tctgcaagtt
      661 ttgtaattgt gctgttctga gtattcataa taactgtccc ctctcatatg atctgataga
      721 aggcccatat caataagcca gattgcctgt ttgattctca ctatcagatt ttttatatat
      781 tgacgcatca atatgttgaa gttatcatac gcgaaatgtg tcattatttc aatgaccaat
      841 tttttgaatt ttttagaggt cagattggat attctgtaaa agaatcctgc ttgcaggatt
      901 ctccgcctgc ggcgggtcgc ttcagcgaca taattcctct ccgtcgcagt gcgatcctgc
      961 ccggagggcc tttttgtgtt ataggaacat tacggagtaa tttcctataa cacaaaaaag
     1021 ccctccgtct gcatgctgct cacatgaaaa cagagggctt cattattcta tttgcttaaa
     1081 tccttgctac gcctgatttt cttgctgctt ctgctacagc tttagctaca gcgtctttta
     1141 cgcgtgggtc aaatgctgcc ggaagaatat attccgctgt taattcttcg tcacttacca
     1201 gacctgcgat tgcatatgct gctgcaactt tcatttcatc gttaacatct gatgctcttg
     1261 cgtccagtac accacggaaa agtcccggga agcagagtac gttgtttacc tggttcgggt
     1321 aatcagaacg tcctgtggat accactgctg cgcctgctgc ttttgcttca tcagggaaaa
     1381 tctcaggtac cgggtttgca catgcaaaga tgataggatc ttttgccatt gtgcggacca
     1441 tatcctgtgt cagtgttcca ggtgcggata caccgatgaa gatgtctgcg cctttgatca
     1501 cttctgcaag agtacctttc tctttgttga agtttgtgat cttagccatc tcttctttga
     1561 tcgggtttaa tccttctctg ccttcgtaaa tcgcaccctt tctgtctgtc atgattacat
     1621 tcttaagtcc catggacatt aacagtttga tgatagcgat acctgctgca ccggctccgg
     1681 aagtaacgat cttcacttcg tcaagcttct ttccaaccag tttcagcgcg ttgataacac
     1741 ctgcaagtgt gataactgct gttccgtgct ggtcatcatg gaagatcgga atatcacagc
     1801 attctttcag tttcttttca atttcaaaac atctcggtgc ggaaatatcc tcaaggttga
     1861 ttcctccaaa ggatccggcc aggaggctta ctgtcttaac gatctcatca acatctttac
     1921 tgcggacgca gagcggaatc gcgtctacac cgccaaattc tttaaagagt acacatttgc
     1981 cttccataac aggcatacct gcttccggtc cgatatctcc cagtccaagt actgcagttc
     2041 cgtctgttac aacagcaact gtattccaac gtcttgtcag atcatagctc ttgtttacat
     2101 ccttctggat ctccagacaa ggctgagcaa cacctggtgt gtaagcaagg cttaaagcat
     2161 ctttggaatc tactttggct cttgatacaa cctcaatttt accttttaag tcataatgca
     2221 tttttaaaga ttcttttgcg tagtccattt tgttttggct cccttctgca ctcttttttt
     2281 gagacgccta cattgtaaca ttaacttggc tcagattcta ctgtttttct gacctttttt
     2341 atcaaaataa ttaaaacatc atctttctgc agaaaattgt gaagatgctg tttcagttac
     2401 gggactttat catctgatat caaggacaaa aagaagcttt atatcattat ttatctgtat
     2461 caaaatgtac ttctgtgccg gcaactgccc gttcatccag gagctgtttg aaggtcctgg
     2521 tctttgcttt ttcttcgtag tcatcggacc agtagtagcc tttcagctca tccatatact
     2581 catagtagtc acaaagataa atatatacag tggaaatgtc tctgtcctgt atcgcatatg
     2641 tgttattgct gtttgacacg cctccatatg gcatgatatc tccattggca tccagcataa
     2701 ctgcaaaata ctttgcttcc ggatcctgca ttttcatagt gatctcaaat ggtgttttcg
     2761 taatggagag aacaccatct ccattttcat ctaccacatt tacatccttt ttaaccgtat
     2821 cggaagtatt tttctctaca tctacgttga acttccagtc acccttcagt gtccatgagt
     2881 tatatctgtt attaccttca attgcctcag gataccgttc aaaatactca ttccacatct
     2941 cagtgaaaaa ctgatgttcc agatccttct gttcttctgt caggctctca taatctgcat
     3001 catctgtgct gattccatgc tctgccattt tctgattata ttcatcccgc agatcctgag
     3061 ggatatccgg ggtagtatcc tggtcatccg gaagagtacc cacaatatct ttcagatcca
     3121 gctccatggc aaacctgtca ggtactttga tgtctttgac ataatcgctg attgccggac
     3181 ctcccacctg aggaaggttc tcatcactgt attcatccat tcccagtgtc tgggctaact
     3241 tgtcaaaact aaagtcctca gaatctacat caatcccttt ttctttgaaa aatgcgttac
     3301 gcacctcata aaattttgtc catcctgcat catccactgt catatcttct acaggaatac
     3361 gcaaaactcc tgcataggtg ttatcatcca gcatttttcc atccagataa
//
LOCUS       DBMDXG010000233         3402 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6320_length_3402_cov_1.312330, whole genome shotgun sequence.
ACCESSION   DBMDXG010000233 DBMDXG010000000
VERSION     DBMDXG010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6320_length_3402_cov_1.312330"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            232..1467
                     /locus_tag="ACZPD3_07000"
     CDS             232..1467
                     /locus_tag="ACZPD3_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1415779.1"
                     /translation="MEFQTKLLHGKAVDNYANGSTVPPVSLANAFAYESSEQLEKVFQ
                     NRAPGFAYTRIANPTVDAFERRVNELEGGIGGVACSSGMSAVTLSLLNILQAGDEVIA
                     GSALFGGTLDLLHDLEAFGIKVHFIPRVEKALIEPFLTDKTRAVFGEIVGNPALNVMD
                     VRETADFLHGKGVPLIVDSTTSTPYLLNPIQYGADVVVHSTSKYINGSGDAISGIIID
                     SGNFSWSPERYPGMKEYKKYGKFAYLVKLRNGIWRNMGGCLAPMNAYLNIIGMETLGL
                     RMERVCNNAFRLAQALEKLEGVSVNYPLLESSPYHELAEKQLSGKGGAILTIRAGSKE
                     RAYKLINHLKYVKIATNIGDVRTLVIHPASTIYIHSTPEAMEEAGVYDDTIRISVGIE
                     DIKDLIKDFTEAVESLGEE"
     gene            complement(1713..2504)
                     /locus_tag="ACZPD3_07005"
     CDS             complement(1713..2504)
                     /locus_tag="ACZPD3_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415780.1"
                     /translation="MSEISYHQQQYIRHQKREKKLVLFLRIFILLFFLVLWEISARTG
                     LIDSFIFSSPVMIWHSFCSMCRDGSIFPHLSVTITETLVSFLFVVVLGAGMAVLLWTC
                     PRLAKITEPYLVVLNSLPKSALAPLLIVWLGANERTIIVCGMSVAIFGSILNLYTGFG
                     EADPEKLKLIETLGGGKKEKLMKIILPSSVPLLLSVMKVNIGLCLVGVIIGEFIGARK
                     GLGYLIIYSSQTFKLTWVLMSIIILCIIAIILYGLLGLIEKRARR"
     gene            complement(2497..3264)
                     /locus_tag="ACZPD3_07010"
     CDS             complement(2497..3264)
                     /locus_tag="ACZPD3_07010"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1415781.1"
                     /translation="MKPLLDITDVSLSYHSLSGETPALSHISFQLMPGEFLAIVGPSG
                     CGKSTLLNLICGLLRPEQGQILLDGTPVTSGDCRIGYMLQKDHLLEWRTIYKNVLLGL
                     EIRRELTAEKLAYVNQLLSDYGLDKFRSAHPSELSGGMRQRAALIRTLALKPELLLLD
                     EPFSALDSQTRLSVSDDIGKILRQEKKTAILVTHDISEAISMADRVIILSPRPAIIRK
                     IVPVCFDLENRTPMASRNAPEFKSYFNLIWKELNHDV"
BASE COUNT         1054 a          689 c          802 g          857 t
ORIGIN      
        1 tgttgatttt agactaaatt atttctatag aaaaataaca aaatatagaa aaaaactata
       61 cgtgtatata aataattgat ataagaaaat ttattgacag atttatgtga aaaatatata
      121 ctactattca tatcgaaaca ctatggaatt gctaaaaaca gggaaacgat atatcacaga
      181 aaacataccg ttagtccgga gattattata ttttacaagg ggaatatgct catggaattt
      241 cagactaaat tattacacgg aaaagcagta gacaattatg cgaatggttc aacagtgccg
      301 cctgtcagcc tggcaaatgc ttttgcatat gaatcttcag aacagctgga aaaagtattc
      361 cagaatcggg caccgggatt tgcatatacc aggattgcga atccgacagt agatgcgttt
      421 gagcgaagag taaatgaatt agaaggcggg attggaggtg tagcctgttc ctccggaatg
      481 tcagcggtta cattatcatt acttaatatc ctgcaggcag gggatgaggt aatcgcagga
      541 agtgcattgt tcggaggtac actggatctg cttcatgatc tggaggcatt cggaatcaaa
      601 gtacatttta taccgagggt agagaaggcg ctgatcgagc cgtttcttac agataagacc
      661 agagcagtgt ttggcgaaat agtaggaaat cccgcactga atgtaatgga tgtccgggaa
      721 acagcagatt ttcttcatgg aaaaggagtg ccgctgatcg tagacagcac aacttcgact
      781 ccttatctgc ttaatccgat tcagtacggt gcggatgtag tggtgcattc tacttctaaa
      841 tatataaatg gaagtggaga tgccatcagt ggaattatca ttgacagcgg aaacttttcc
      901 tggagtccgg aacgttatcc tggaatgaag gaatataaga aatacggaaa gtttgcttat
      961 cttgtgaaac tgaggaacgg aatctggcga aatatgggcg gctgtctggc tccgatgaac
     1021 gcttatctga atattatcgg aatggagaca cttggtcttc gtatggagcg ggtctgtaat
     1081 aacgctttcc ggcttgccca ggcactggag aagctggaag gtgtatctgt caactatccg
     1141 ctgctggagt ccagtccata tcatgaactg gcagaaaaac agcttagtgg aaagggtgga
     1201 gcgattctga ctatccgtgc aggttccaaa gaacgggcgt ataagctgat caatcatctg
     1261 aaatatgtaa agattgcaac caatatcggt gatgtgagaa ctctggtgat tcatccggcg
     1321 tctacaattt atatccacag tacgccggag gcaatggaag aggctggagt gtacgacgac
     1381 acaatccgta tcagtgtggg tatcgaagat ataaaagatt tgataaagga tttcacagag
     1441 gcagtggaga gccttggaga agaataattc atgatataca cattatcagg cgctttggtc
     1501 ggaacatgat agtaaccgga cagaggcact ggtttaaaaa cagaataact gacagacagt
     1561 cagagacaag agaagccccg gaagttcagg agtgtccagt taagtggaac tcgatgatct
     1621 tccgggcttc tttttgcagc aaaatttttt cagatattga atctgtttcc gaaaccgttt
     1681 cgttataaaa aatagcggtt aaaaattaac aactatctgc gggcgcgttt ttcaatcagt
     1741 ccgaggagtc cgtacaggat aatagcgatg atgcacagaa tgataatgga catcagtacc
     1801 caggtaagct taaaggtctg gctggaatag atgatcaggt agccaagccc ttttctggca
     1861 ccgataaatt cgcctatgat aacaccaacc agacacagac caatattaac tttcataaca
     1921 ctgagcagta acggcacaga agacggaaga ataatcttca taagcttttc tttcttccca
     1981 ccgcccagcg tctcaatcag cttcagtttt tccgggtcag cttcgccaaa acctgtatac
     2041 agattcaaaa tcgaaccaaa gatagcaacc gacataccgc agacaataat cgtccgttcg
     2101 ttggcaccaa gccatacaat aagcaaaggt gccagagcag atttcggaag gctgttcaat
     2161 actacgagat aaggctctgt gatcttcgca agtctgggac aggtccacag cagaactgcc
     2221 atccctgcac ccagaacaac aacgaacaga aaactgacca gtgtttccgt aattgtaaca
     2281 gagagatggg gaaaaataga gccgtctctg cacatactgc agaagctgtg ccagatcatt
     2341 accggactgc tgaagataaa agaatcgatc agtccggtcc tggcggagat ttcccacaac
     2401 accagaaaga aaagaagaat aaatatccgc aaaaaaagaa caagtttctt ttctcttttc
     2461 tgatgtcgga tatactgttg ctgatgatat gagatttcag acatcatgat tcagctcctt
     2521 ccagataaga ttgaaataag atttaaattc aggcgcattt ctggaagcca taggagtacg
     2581 gttctccaga tcaaaacaaa ctggcacaat cttacgaatg atggcaggcc ttggagaaag
     2641 aatgataacc cggtctgcca tgctgatcgc ttctgaaata tcatgggtga caagaattgc
     2701 agtctttttt tcctgacgca ggattttgcc gatatcatca cttacgctga gtctggtttg
     2761 agagtcgagt gcagagaacg gctcatccag cagaagaagt tccggtttta atgccagagt
     2821 acggatcagt gcagctctct ggcgcattcc tccggacaat tcggacggat gtgcagagcg
     2881 gaatttgtcc aaaccgtaat ctgagagaag ctgatttaca taagcaagtt tttcggcagt
     2941 gagttccctg cggatttcca gtccgagaag aacattttta taaattgtcc gccactccag
     3001 cagatgatcc ttttgcagca tatatccgat acggcagtca ccggaagtta ccggcgtgcc
     3061 gtccagaaga atctgtccct gttctggccg gagcagacca cagatcagat tcagcagcgt
     3121 ggactttcca cagcctgacg gcccgacgat ggcgagaaat tcacctggca tcagttgaaa
     3181 ggatatatga gaaagggcag gggtttcccc tgataaactg tggtaggaga gactgacatc
     3241 tgtaatatcc agcagtggtt tcatacagat acctccatat tcggtgaata gtaacgaata
     3301 tttgttactg ttcacacacc actatcccgc gaggtgtttt ggcatgaata tgccaaaatc
     3361 ccgagacata caagccaaaa ttctattgcc gcaggcaatc tg
//
LOCUS       DBMDXG010000234         3397 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6330_length_3397_cov_1.642900, whole genome shotgun sequence.
ACCESSION   DBMDXG010000234 DBMDXG010000000
VERSION     DBMDXG010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_3397_cov_1.642900"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(70..546)
                     /locus_tag="ACZPD3_07015"
     CDS             complement(70..546)
                     /locus_tag="ACZPD3_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1415782.1"
                     /translation="MKLSTRARYGLKALIDLGLHSANEAISLQSIAERQDISTSYLEQ
                     LMAMLKKAGLVKSSRGAYGGYQLGKPADEISVGEVLRVLEGSLEAAACPGIENDGTCH
                     GSDVCVAKLVWKRINDSITSAVDTLMLGQLIEESRRVHEEKMESQGMEENVPDILK"
     gene            733..2064
                     /locus_tag="ACZPD3_07020"
     CDS             733..2064
                     /locus_tag="ACZPD3_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR1415783.1"
                     /translation="MSKKLFIKGTLLLTFAGLLSRLMGFFYRIFLSHTIGAHGLGIFQ
                     LILPLQILIMSICASGIQTAISRLTAAEKVSKNPSRHISDYFVVGTVFSVVFSLVFSW
                     FLYSYADFWAVQILKESQTSSLIRILSLSIPLSTLHTCISSYYLGRKQAGFPAGVQLL
                     EQLFRTGGCYILYLICASQGRNITPAIAVGGNLIGEIASSFISLFAVSMHFKVTHYNI
                     RNIRRPLSVLRKLLHISVPLTMNKILLTLLGSIEVILIPARLQMSGFTPKDSLSVYGI
                     FTGMALPLILFPATLTNSAAAMLIPSITQLQTLGYQKRIQYVIGRIFRYCLLLGGSCL
                     VFFLFLGEFLGNFLFHSQTAGIYIHTMSYICPFLYLNTTLTSVLNGLGKSGICLLHSV
                     ISVCIRISFVLFAIPVLGIRGYLYGILCSELALSILHSLQILQNNYSNPRK"
     gene            2329..3360
                     /locus_tag="ACZPD3_07025"
     CDS             2329..3360
                     /locus_tag="ACZPD3_07025"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1415784.1"
                     /translation="MGFDFIKKLPTPEEIRNQYPIDAEIQAVKDARDKELRDVFTGKS
                     DKFLAIIGPCSADNEDAVLDYLTRLRNVQEKIADKVLIVPRVYTNKPRTTGEGYKGMV
                     HQPDPEKQPNLLAGLVAIRKMHIHAIRTSGMTCADEMLYPENYRYLSDLLSYVAVGAR
                     SVEDQQHRLTVSGMEVPAGMKNPTSGDLAVMLNSVVAAQGGHRFIYRSWEVETTGNEL
                     AHTILRGAVNKHGEAIPNYHYEDLRLLWEKYQEKNLKNPAVIVDTNHSNSNKQYDQQV
                     RIAKEVLHSRQVDPELHTLVKGLMIESYIEPGNQKIGCDHIYGKSITDPCLGWEESER
                     LLYTIAEMC"
BASE COUNT          884 a          794 c          692 g         1027 t
ORIGIN      
        1 ccggtgctgt tttagttgtt gctgcattgt ccagataaat catttttccc attttcttgt
       61 cctcctgttt tatttaagta tgtccggtac attttcttcc atgccctgac tttccatttt
      121 ttcttcatgt actcttctgc tttcctcgat cagctgacca agcatcaggg tatcgacagc
      181 gcttgtaata ctgtcattga ttctcttcca gacgagtttt gccacacata catcactgcc
      241 atggcaggtt ccgtcattct caatcccggg acaggctgct gcttccaggc tgccttccag
      301 cacacgcagg acttcaccga ccgagatctc atctgccggc ttgccaagct ggtatccgcc
      361 gtaagcccct ctgctgctct ttacaagacc ggccttctta agcatggcca taagctgttc
      421 gaggtagctt gtggaaatat cctgtctctc cgcaatactc tgtaaagata tcgcttcatt
      481 cgcactgtgc aaaccaagat ctatcaatgc tttcaagcca taacgggctc ttgttgagag
      541 tttcattttc tcacctcgtt ttttaattcc cagtgtttcc ctcggatttc atgattactt
      601 taccagaaac aaaccgttct gtcaaggctg ttttgttcat atattgctgt aaaggcgttt
      661 cggcaggtta ctgtttttta gtcaagcgga tattcgcacc tgctgcgcat gaaaaggtct
      721 ggatttttca ttatgtcaaa aaaacttttt atcaaaggaa ctttacttct tacatttgcc
      781 ggtttgttaa gcagacttat ggggttcttt tacagaatat tcctgtcaca cactattggt
      841 gcacatgggc ttggtatctt tcagcttata cttccactgc agattctgat catgtccatc
      901 tgcgcttccg gtatccagac tgcaatctcc cgcctcactg ccgcagaaaa agtttccaaa
      961 aatcccagtc gtcatatctc agattatttt gtcgtgggaa ctgtattttc tgttgttttc
     1021 tccctggtat tctcctggtt tctttacagc tatgcggatt tctgggcagt acagattctg
     1081 aaagagtcac agacctccag tctgatacgg attctttctc tgagtattcc gttgagtaca
     1141 cttcatacct gtatcagcag ctattatttg ggaagaaaac aggccggatt tcccgcagga
     1201 gtccaacttc tggaacagct tttccgcaca ggcggatgct atattcttta tctgatctgt
     1261 gcatcgcaag ggcgaaacat cactccggca attgcagtcg gcggaaatct tatcggggaa
     1321 atagcctcat cttttatttc tcttttcgca gtcagtatgc attttaaggt aacacattat
     1381 aatatccgga atatcagaag accgctttct gttctcagaa aattgctcca tatttccgtt
     1441 cctcttacca tgaataaaat actactcacc cttctgggaa gtattgaagt gattcttata
     1501 cctgcaaggc tgcaaatgtc aggatttact cccaaagatt ccctgagtgt ttacggaatc
     1561 tttacaggga tggcacttcc gcttattctt ttccccgcta cgcttacaaa ctcggctgcg
     1621 gccatgctga ttccctccat tacccagctg cagactctgg ggtatcaaaa gcgaatccaa
     1681 tatgtgatcg gcaggatttt ccgatactgt ctgctgctag gcggatcctg cttggttttt
     1741 tttctgtttc ttggagaatt tcttggaaat tttctgtttc acagtcagac agccggaatc
     1801 tatatccata ccatgtccta tatctgtcct tttctgtacc tgaacaccac tctcaccagc
     1861 gtactcaacg gccttggaaa atccggcatc tgcctgctgc acagtgtgat cagcgtctgc
     1921 atccggatca gtttcgtgct gtttgcaatc cctgttctgg gaatccgggg atatctgtac
     1981 gggattctct gcagcgaact ggcgctcagc atcctgcaca gtttacaaat actgcaaaac
     2041 aactatagca atccccgtaa ataatgcata ttgatcaaga gacttgatag ttactattca
     2101 cactccacta tcccgcgagg tgttttggca tggatatgcc aaaattccat tgccgcaggt
     2161 aatctggaat gaattttggc tacgttactg agaacgaagt gaacagtaac gcttgatatg
     2221 atcgcagaaa gtcatccgaa aatattttgg ctatcgacat tcagatagga atggtttata
     2281 ataaattccg aaaaaagcaa gacaaataga cacaaaggag atattactat gggattcgat
     2341 tttatcaaaa aattaccaac ccctgaagaa atccgtaatc agtatcccat tgatgctgaa
     2401 attcaggctg ttaaagatgc aagagataaa gaactgcgtg atgtttttac cggaaagtcc
     2461 gataaattcc ttgcaatcat cggtccttgt tcagcagata atgaagatgc tgttctggac
     2521 tatcttacca gattacgcaa tgttcaggag aaaatagctg acaaggttct gattgttcca
     2581 cgtgtttata caaacaaacc aagaacaaca ggagaaggct ataaaggtat ggttcaccag
     2641 ccggatcccg agaaacagcc aaatcttctt gcagggcttg tagcaatccg taaaatgcat
     2701 atccatgcaa tccggaccag tggtatgacc tgtgcagatg agatgcttta tccggaaaat
     2761 taccgttacc tttctgacct gctttcttat gtggcagtag gtgcacgttc tgtggaagac
     2821 cagcagcatc gtcttaccgt cagcggcatg gaagttcctg caggtatgaa aaatccaacc
     2881 agcggcgatc ttgcagtcat gctcaattcc gttgtagcag ctcagggtgg acatcgtttt
     2941 atctacagaa gctgggaagt agaaacaaca ggtaatgaac tggctcatac aattctccgc
     3001 ggtgccgtaa ataaacatgg ggaggcaatc ccgaactatc attacgagga tcttcgtctt
     3061 ctctgggaga aatatcagga gaaaaacctt aaaaatccgg ctgttatcgt tgataccaac
     3121 cactctaatt ctaataaaca atatgaccag caggttcgta ttgcaaaaga ggttctccac
     3181 agccgccagg tagacccgga acttcatact ctggtaaaag ggcttatgat cgagagttat
     3241 attgaaccgg gaaatcagaa gatcggatgt gatcatattt acggaaaatc tattaccgat
     3301 ccatgtcttg gatgggaaga atccgaacgt cttctgtata caattgccga gatgtgttga
     3361 tccgttgtta ttgtttataa acaacattcc atactct
//
LOCUS       DBMDXG010000235         3389 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6349_length_3389_cov_1.435523, whole genome shotgun sequence.
ACCESSION   DBMDXG010000235 DBMDXG010000000
VERSION     DBMDXG010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6349_length_3389_cov_1.435523"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="ACZPD3_07030"
     CDS             <1..230
                     /locus_tag="ACZPD3_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415785.1"
                     /translation="KFSENIQEQHLQNIKNIKFRTGNNIGIKSFLIIYEDCYRSRNRT
                     ENKNISERPFMTYDVQINIFKKYKKGIDKQK"
     gene            319..939
                     /locus_tag="ACZPD3_07035"
     CDS             319..939
                     /locus_tag="ACZPD3_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR1415786.1"
                     /translation="MKQILCFGDSNTYGLIPGTTNQRYGWGTRWTSILDDKVRTKGYR
                     VIEEGLCGRTTVFDDPFRTERRGTEMLPAILESHRPVDTIVLMLGTNDCKSVYSATPE
                     VIGQGIEQLLDQINTVNPDAKILLVSPIYLGERIWEEDFDPEFDKNSIEVSWNLPRVY
                     EKIARRRNISYLPASEFARPGEADQEHLDELGHSRLADAIYEKLAG"
     gene            1118..1777
                     /locus_tag="ACZPD3_07040"
     CDS             1118..1777
                     /locus_tag="ACZPD3_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1415787.1"
                     /translation="MDFEFIREQTPLYIEAAKLTLHMAVIGVLLAIVVGLVCSMIKYF
                     RIPVLRQIVECYIELSRNTPLLIQLFFLYFGLPKIGIVLSSEQCAITGLTFLGGSYMA
                     EAFRSGLDNVPPIQVESALSLGMSKSQVFTNIILPQAVSNSIPAFCANAIFLIKETSV
                     FSAVALADLMFVTKDLIGIYYKTDEALFMLVIAYLIILLPISLICSFVERRVRYAEYG
                     D"
     gene            1758..2435
                     /locus_tag="ACZPD3_07045"
     CDS             1758..2435
                     /locus_tag="ACZPD3_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1415788.1"
                     /translation="MQNMGIEVLFKGINSLRLWQGLWVTIRIALISMVFSIILGFLLG
                     MVMNIPNKIIKFICRIYLEIVRIMPQLVLLFLVYFGAAKHLGMNLSGETAAVIVFTFW
                     GTAEMGDLVRSALISIPKHQYDSGYGLGLNKWQVYLYIILPQTIRRLLPSAVNLLTRM
                     IKTTSLVALIGVVEVLKVGKQIIDASRYTNPTAALWVYGAVFFMYFIICFPFSRLSRV
                     LEKKFAD"
     gene            2457..3212
                     /locus_tag="ACZPD3_07050"
     CDS             2457..3212
                     /locus_tag="ACZPD3_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1415789.1"
                     /translation="MGQPILEVEHLKKSYVNNVPILDDVSFSLQKGEVVVIVGPSGCG
                     KSTFLRCINRLEEIDTGVLKLNGVSYEKEKKNISKVREKIGMVFQSYDLFPNMTILNN
                     LLLAPMKVQKRNKEEVKKEAEQLLDRVGLLDKKDNYPRQLSGGQKQRVAIVRAMLMHP
                     EIMLLDEITAALDPEMVREVLQVVLELAKTGMTMLIVTHEMEFARSVADRVIFMDKGN
                     IVEENTPEQFFDNPETDRAKQFLNSFHYETVKK"
BASE COUNT         1094 a          627 c          761 g          907 t
ORIGIN      
        1 aaaaattttc agaaaatata caggaacaac acctgcaaaa tataaaaaat ataaagttca
       61 ggactggaaa taatatcggt atcaaaagtt ttttgattat ttacgaggat tgctataggt
      121 cccgaaaccg cacggaaaat aaaaatattt ctgagcgtcc ctttatgact tatgacgtac
      181 aaataaacat tttcaaaaaa tacaaaaaag ggattgacaa acaaaaataa agattgtata
      241 gtattcctat caaatcaata tgaaaacagg gaaagcaaaa aatcccatta aaaatatcag
      301 ataagaaggt gacaagacat gaaacaaatc ttatgtttcg gtgattccaa tacatacggt
      361 ttaatcccgg gaactactaa tcagagatac ggatggggta cccgctggac cagcatcctg
      421 gatgacaaag taagaacaaa aggctacaga gtaatcgaag aaggactctg cggaagaacc
      481 accgtattcg atgatccatt ccgtacagaa agaagaggaa cagaaatgct tccggcaatc
      541 cttgagagcc acagaccagt agacactatc gttctcatgc ttggcacgaa cgattgtaaa
      601 agcgtataca gcgcaacccc ggaagtgatc ggacagggga ttgaacagct ccttgatcag
      661 atcaacactg taaacccgga tgccaaaata ttactggtat ctccaatcta ccttggggag
      721 agaatctggg aagaggattt tgatccggaa ttcgataaga attctatcga agtatcatgg
      781 aatctgcccc gggtgtatga gaaaatcgcc agaagaagaa atatttctta tcttccggct
      841 tcagaatttg cccgaccggg agaagccgat caggagcatc tggatgaact gggacatagc
      901 agacttgcag atgcaattta tgaaaagctt gcaggatgaa gcctgaaatg ttatgatagt
      961 tcacagatgt gcagatgtaa cgctttaact aaatcaagta aactacctgc tttaaccgaa
     1021 atagcagtgg attgaaaagt aataagcggc acaggcacac cagagcgttc ttcaaatatg
     1081 ctctaaatca taataataga aggagaataa agtaaccatg gattttgagt ttatccgcga
     1141 gcagacacct ctttatattg aggcagcaaa gcttacactt cacatggcgg tgattggtgt
     1201 gctgcttgcc attgtggttg ggcttgtctg cagcatgatt aagtatttca ggattcctgt
     1261 acttcgtcag atcgtggaat gttacattga actgtccaga aatacaccat tgttgattca
     1321 gttgtttttc ctgtatttcg gtcttccgaa aatagggatt gtcctcagtt cagaacagtg
     1381 tgcgatcaca ggactgacct ttctcggagg cagttatatg gcagaagcct tccgaagcgg
     1441 cctggacaat gttccaccca ttcaggtgga atccgcatta agccttggaa tgagtaagag
     1501 tcaggtattt acaaacatta tcctgcccca ggcggtatcc aattctattc cggcattctg
     1561 tgcaaatgcg atctttctta ttaaggaaac ctccgtattc agtgcagtag cacttgcaga
     1621 cctgatgttt gtgacaaaag acctgatcgg aatttattac aaaacagatg aagccctgtt
     1681 tatgttggta atcgcatatc tgatcatact tttaccaatc tcacttatct gttcatttgt
     1741 agaaaggaga gtccgctatg cagaatatgg ggattgaggt tctttttaag ggaatcaact
     1801 cattaagact ctggcagggc ttgtgggtaa caataagaat tgcactgatt tccatggtat
     1861 tttccataat ccttggattt cttcttggga tggtaatgaa tatcccaaat aaaatcatta
     1921 agtttatctg ccgcatttac cttgaaatag taagaatcat gccgcagctg gtgcttttgt
     1981 tcctggtata tttcggagcg gccaagcatc tggggatgaa tctttccggt gagacagcgg
     2041 cggtcattgt atttacattc tggggaactg ctgaaatggg tgatctggtg cgaagtgcgc
     2101 tgatctccat tcctaaacac cagtatgaca gcggatacgg tcttggatta aacaaatggc
     2161 aggtctatct ttatattatc cttccgcaga ctataagaag acttcttcca tccgcagtga
     2221 atcttcttac ccgaatgatc aagaccacat cactggtagc tcttatcgga gtagtagagg
     2281 tgttaaaagt tggaaaacag atcatagatg catcacgtta tacaaatccg acagcggccc
     2341 tctgggtata tggtgcagta ttctttatgt attttatcat atgttttcca ttttccagac
     2401 tctccagagt actggaaaag aagtttgctg attaatagag aaatgaggat aaatgaatgg
     2461 gacagccgat tttagaagta gaacatttaa agaaatcata tgtaaacaat gttcctattc
     2521 tggatgatgt ttccttctct ctgcaaaagg gagaagtggt agtgatcgta ggaccttccg
     2581 gatgtggaaa aagtaccttc ctgcgatgta tcaaccgcct ggaagagatt gacaccggtg
     2641 tactgaagct taacggtgta agctatgaga aagaaaaaaa gaatataagc aaagtcagag
     2701 agaagatagg aatggttttc cagagctatg atcttttccc gaatatgaca attttaaata
     2761 atcttcttct ggcaccaatg aaagtacaga aaagaaataa agaagaagtg aaaaaggaag
     2821 cggagcagct tcttgacaga gttggtcttc tggataagaa agataattat ccaaggcagc
     2881 tttccggtgg tcagaaacag cgagttgcca ttgtacgtgc catgctgatg catccggaga
     2941 tcatgcttct ggatgagatt acagcagcac ttgatcctga gatggtaaga gaagtacttc
     3001 aggtagtact ggaacttgca aagacaggaa tgaccatgct gatcgtcact catgagatgg
     3061 aatttgcccg ttccgtagca gacagagtga tctttatgga taagggaaac attgtagaag
     3121 agaacactcc ggaacagttc tttgacaatc cggagactga cagggcaaaa cagttcctga
     3181 acagtttcca ttatgaaaca gtgaaaaaat agaatagagg aaaacagaat attttataaa
     3241 tcctgaaatg gatgataata tccatgaaat agcaggatat cataagagct agtacatcgt
     3301 ttcgtaaata actataccaa tcgcgtagga tgcggattcg agtgtgctgg tataaaaacg
     3361 caggcgtagc gggctacgct gaggctttt
//
LOCUS       DBMDXG010000236         3384 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6358_length_3384_cov_1.651843, whole genome shotgun sequence.
ACCESSION   DBMDXG010000236 DBMDXG010000000
VERSION     DBMDXG010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6358_length_3384_cov_1.651843"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            753..1307
                     /locus_tag="ACZPD3_07055"
     CDS             753..1307
                     /locus_tag="ACZPD3_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR1415790.1"
                     /translation="MNIFELFILAIGLSMDAFAVSVCKGLSLGRINAKHMCIAGAWFG
                     GFQALMPLVGYFGGRFFADKVTRYSHWVAFVLLVFIGAGMIKESKEEEHVNADMDIKS
                     MFILAVATSIDALAVGVTFAFLKVEIVSAVSFIGVITFVCSAAGVKIGSLFGMKYKSK
                     AELCGGIILILIGTKILLEGLGMI"
     gene            1420..3015
                     /locus_tag="ACZPD3_07060"
     CDS             1420..3015
                     /locus_tag="ACZPD3_07060"
                     /inference="COORDINATES: protein motif:HMM:NF017967.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HIR1415791.1"
                     /translation="MKKVKTAAALLCSACLVLSGTAVPTMADSVKVVTLGADLTQDQK
                     NTMMKYFNVDSNQVQILTITNQDERDHLSAYVPLEQIGTRTVSCAYVKPTQSGGIKVK
                     TANLNWVTCNMIATSLSTSGVKNCEVIAACPFEVSGTGALTGIQMAYETATGEQLDST
                     KKELATEEMVVTGNLADEVGKNDATTVMNNSKIQVIKDNVQNVDDIYNIVVNVAQQNN
                     VNLDSDQINKIVELLKQIAQQEYNYDDVKATLEQVEQNTSGDNDELGDIDDEEDDTVN
                     AGDSADGDDILNNVDNSALGGDIVESSTENPSLEEDSGLIEDDGDDQSDGDLSETEEP
                     QEPDGDETTDDSTLGNTDEGDTDSDEGTDDTENSATSDELDTSALTEDQKTMFDKAEN
                     FCKGEYEGDAAALTAAMEDDTATASVILDAENGAALTKDVEKAYLKILTEGTASYQAD
                     GTEIYMSTELNMVDKSMKEIFGLSADAQASPELGELSDDDRQTLYNETMKFFEKLYGE
                     STETYDTTDADSTETAGGEENAE"
     gene            3005..>3384
                     /locus_tag="ACZPD3_07065"
     CDS             3005..>3384
                     /locus_tag="ACZPD3_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415792.1"
                     /translation="MQNNQSIMQISENKDGSAKVLFRIRKAEMTDVDEIMAVMHEAKN
                     DKEHPDWFVSDDEEYVRTHIEEQGFVIVAQTADGSVAGFFIIKYPENREDNLGTYLDF
                     DEEQLSHVAVMDSAAVCCAYRGNGL"
BASE COUNT         1107 a          575 c          841 g          861 t
ORIGIN      
        1 aagcctcagc gtagcccgct acgcctgcgt ttttatacca gcacactcga atccgcatcc
       61 tacgcgattg gtatagttat ttacgaaacg atgtactaga attcgaagaa tcgccatata
      121 agtcaacaaa tgccgggtaa actgatgact agaatcgaag aatcgccata taagtcaaca
      181 aatgccggga aagcagatga ctggaattcg aagaatcgcc atataagtca acgaatatcg
      241 ggaaagcata tgactggaat ttgaagaatt gtcatataag tcaacgtatg tcagggaaac
      301 tgatgactgg aatttaaaga atcatcattt gagtcaatac atgattgata tttgttgact
      361 aggaggtgca tatttttggc atcgtgaagc gtgttgatgt gaacaggaac gaggtggtgc
      421 ttatgagctc cagactgaca aaaatctact tgataaatat agatcaatat gataaaataa
      481 ggccattctc cgcgagggag taatcggcat attccggaac atttttcaga tatgttttga
      541 agagtatgta gtactatcga cataatggtg ccatatgcac ccggtattac agtaaacgat
      601 gagactcatg gacagttaaa acagctgtct gtgtgtctct ttttttatgc tgcaggaata
      661 ttacagcagc tcagataaca taaaaaagtc ttccgggaag ggttctatgt tactgaaaaa
      721 ttattttata caggaaaagg agcaaaaaac acatgaatat ttttgaactt tttatactgg
      781 cgataggcct gtctatggat gcatttgcag tatctgtctg taagggcctt tccctgggca
      841 gaatcaatgc aaagcatatg tgtatcgcag gcgcatggtt cgggggattt caggcattaa
      901 tgccccttgt gggatatttt ggcggacgat tctttgcaga taaggttact cgatacagtc
      961 actgggtagc ttttgtcctt ctggtgttca ttggtgccgg catgatcaaa gaatcgaaag
     1021 aggaagaaca tgtaaatgcg gatatggata taaaatccat gttcatcctt gcagtggcta
     1081 ccagtattga tgcactggca gtgggagtga catttgcttt tctgaaagta gagatcgtat
     1141 cagcagtatc ctttatcggt gtgatcacct ttgtctgttc agcagcaggg gtaaagatag
     1201 gcagtctttt cggaatgaaa tacaaatcca aagcggaatt atgcggaggt attatcctta
     1261 tcctcatagg tacaaaaatc ctgctggaag gactgggaat gatataaata ataattacaa
     1321 atctcaactc aaacgtgtta taatactact attaatgaac aggcatgaaa tgattatccg
     1381 cctgaataat ttaaataaca tatcagggag gtttctatta tgaaaaaagt aaaaacagca
     1441 gcagcacttt tatgcagtgc atgtctggta ctttcaggaa ctgctgtacc gacaatggca
     1501 gattcagtga aggttgtgac gctgggagcc gatcttactc aggaccagaa gaatacaatg
     1561 atgaaatatt ttaatgtaga cagtaaccag gtacagattc tcactatcac taatcaggat
     1621 gagagagatc atctgagtgc ttatgtaccg ttagagcaga tcggaaccag aacagtgagc
     1681 tgtgcatatg taaagccgac tcagtcaggc ggtatcaaag taaagacagc caatcttaac
     1741 tgggtaacat gtaacatgat cgcaacgtca ctttccacat caggtgtcaa gaactgtgaa
     1801 gtgatagcag cctgcccgtt tgaggtatcc ggaaccggag cgcttacagg tattcagatg
     1861 gcatatgaga cagctacagg ggagcagctt gacagtacga agaaagaact tgctacagag
     1921 gaaatggtag taacaggaaa tctggctgat gaggttggaa agaatgatgc aactactgtt
     1981 atgaataact ccaagataca ggttatcaaa gataatgtcc agaatgtgga tgatatctat
     2041 aatatcgtag taaatgttgc tcagcagaat aatgtaaatc tggattctga ccagattaat
     2101 aagatcgtag agcttctgaa acagattgcg cagcaggagt acaattatga tgatgtaaaa
     2161 gcaacactgg agcaggtaga acagaataca tccggtgata atgatgagct tggtgatatt
     2221 gatgatgaag aggatgacac tgtaaatgca ggtgattccg cagatggaga tgatatcctg
     2281 aacaatgtgg ataacagtgc tttaggcgga gatatcgtag agagttccac agagaatcca
     2341 tcgctggagg aggattcagg tctgattgag gacgacgggg acgatcagag tgacggagac
     2401 ctcagcgaaa cagaagaacc gcaggagccg gatggtgacg agactacgga cgacagtaca
     2461 cttggaaata cagatgaagg tgatacagac tccgatgaag gaacagatga tacagaaaat
     2521 agtgccacat cagatgaact ggatacttct gcactgacag aagatcagaa gaccatgttt
     2581 gataaagctg agaatttctg caagggagaa tacgaaggtg atgcagctgc tcttacagca
     2641 gctatggaag atgatacagc aacagcttct gtaattcttg acgctgaaaa tggtgcagct
     2701 cttactaagg atgtggagaa ggcatatctg aaaattctta cagaaggcac agcttcttat
     2761 caggcagatg gaacagagat ttacatgagc acagagctca atatggtaga taagtccatg
     2821 aaggaaatct tcggattaag tgcagatgca caggcaagtc cggagcttgg agaactttca
     2881 gatgatgaca gacagacctt atataatgaa acaatgaaat tctttgagaa actctacgga
     2941 gaaagcacag agacttatga tacaacggat gcagattcca cagagactgc aggcggcgag
     3001 gaaaatgcag aataatcaaa gcatcatgca gatttcagag aataaggatg gttctgcaaa
     3061 ggtattgttc aggatacgaa aagcagagat gacagatgtg gatgagatca tggctgttat
     3121 gcatgaagcg aagaatgata aagagcatcc ggactggttt gtatctgatg atgaggaata
     3181 tgtcaggaca catatagaag aacagggctt tgtgatcgtg gcacagacag cagatggttc
     3241 tgttgcgggt ttcttcatta ttaaatatcc tgaaaacagg gaagataatc tgggaactta
     3301 tcttgatttt gatgaggaac agctttccca tgttgcagtc atggattcgg cagctgtgtg
     3361 ttgtgcatac aggggaaacg ggct
//
LOCUS       DBMDXG010000237         3383 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6361_length_3383_cov_1.495734, whole genome shotgun sequence.
ACCESSION   DBMDXG010000237 DBMDXG010000000
VERSION     DBMDXG010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6361_length_3383_cov_1.495734"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="ACZPD3_07070"
     CDS             complement(<1..1429)
                     /locus_tag="ACZPD3_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1415793.1"
                     /translation="MVEDTMFSGESKNIEYKVSLPDKSEKYMKTIVAFANTQGGKLIV
                     GVDDKTHQIVGVENDVLFQLMDGIANAVSDSCVPQIIPDIEPQTVNGKTVIVVSVEAG
                     KNRPYYLKSKGKDNGTYIRVAGTSRQAFPEKIKELEMEGARISWDELTCVGYPVSEEA
                     TEKLCQDIEKFRKKAGMSEHSVKKEQLINWKILKQSEGQFLATNAYALLTSDYFSFSK
                     TQCAVFKGTDRAVFLDKREFTGPIYTQIEEAVDFVLRNIRLGATIDGLVRKEKYELPP
                     EAIREMIINAHCHRNLLDESCIQVAVYDDRLEVTSPGGLYNGLTYEEIMNGHSKIRNK
                     GIANIFSQMGLVEAWGSGIKRILNAAEEYGLPKPKFQEFDNMFRVELFRNSFPMINEK
                     ENIGEASEKHRRSIGETSEVAWRTDLNDTQQKIIKLLSEDHQLSAVKLAEKIGVASRN
                     IENNIKKLKEYGILIRHGSPKNGYWE"
     gene            complement(1470..1883)
                     /locus_tag="ACZPD3_07075"
     CDS             complement(1470..1883)
                     /locus_tag="ACZPD3_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415794.1"
                     /translation="MKKRWFISLIIGIIMVISGCLGYLQYGRDMDVYGSYAMTADNYH
                     EERLTVVVNKLYVADQKVCAEEIVKRCRENSFKSVRFSYDQSIPNALYVTVYSSKRQA
                     EKGKQMFSFSYLPEDSDETYNIVNDSDKFMLKLEK"
     gene            complement(2072..2827)
                     /locus_tag="ACZPD3_07080"
     CDS             complement(2072..2827)
                     /locus_tag="ACZPD3_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR1415795.1"
                     /translation="MSRKNHKMIDGRLLQTNKKYSQLKMKQKEKIAEWMFQATRDYYM
                     KKCTFPSDKHLEEVVDSVYEKIEDAEIWIPYGEVFKHYKSKRSDINKRVRKSLNEKEE
                     SRIEKVCFMNMCMIQDHKGNVLALDKVNDSYTGTTFPGGHVEANEIFQKSMIREVWEE
                     TGLTVEAPKLGGLYHWHKSGVHYVITLYKADKFTGELKSYEEGRVYWIPLEELKTKEL
                     ATGMEHVLRILESEKVDECYMHLEADGYVGDLY"
     gene            3111..3260
                     /locus_tag="ACZPD3_07085"
     CDS             3111..3260
                     /locus_tag="ACZPD3_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415796.1"
                     /translation="MAKRKRNIQILFCVSPEEKKLIRRKMIESKTKNMGAYGVLFIFC
                     FRESL"
BASE COUNT          908 a          801 c          513 g         1161 t
ORIGIN      
        1 tctcccagta accattcttt ggagagccat gtcttataag aataccatac tcttttaact
       61 tcttaatatt gttctctata tttcgactgg caacaccgat tttttcagct aacttaaccg
      121 ctgataattg atgatcttct gaaagcaatt ttataatctt ttgctgtgta tcgttgaggt
      181 ctgttctcca cgctacttcc gatgtttctc cgatgcttct ccgatgcttc tccgatgctt
      241 ctccgatgtt ttctttttca tttatcatcg gaaatgaatt acgaaacagc tctacccgaa
      301 acatattatc gaattcctga aatttcggct tcggaagtcc atactcctct gctgcattaa
      361 ggattctttt aattccgctt ccccatgctt ctacgagtcc catctgactg aatatattgg
      421 caattccttt attccggatc ttagaatgac cattcatgat ttcctcataa gtcaatccgt
      481 tatataatcc gcctggagaa gtcacttcca gtctgtcatc ataaactgcg acctgaatac
      541 aggattcatc caacaggtta cggtgacaat gggcgttaat gatcatttcc ctgattgctt
      601 ccggtggtag ctcatatttc tcttttcgca ccaatccgtc aattgttgct ccaagtcgaa
      661 tatttctcaa tacaaaatct actgcttctt cgatttgtgt atagattggt cctgtaaatt
      721 ctctcttatc aagaaatact gcacgatctg ttcccttgaa taccgcacat tgggttttag
      781 aaaatgagaa ataatctgat gtcagcagcg cataggcatt cgttgccaga aactgtccct
      841 cactctgctt taagatcttc cagtttatca gctgctcttt cttcacagaa tgctctgaca
      901 ttcctgcttt tttccggaac ttttcaatat cctggcaaag tttctctgta gcttcttctg
      961 aaactggata tcctacacag gtaagttcat cccaggagat tctggctcct tccatctcca
     1021 gttcttttat tttctctgga aatgcctgtc ttgatgtacc tgctacacgg atataagtgc
     1081 cattatcttt tccctttgat tttagataat atggtctatt ctttcctgct tctactgata
     1141 caacaataac tgttttcccg tttaccgtct gcggctcaat atcaggaatg atctgtggca
     1201 cacaggaatc agaaactgca ttggcaattc catccatcag ctgaaacaac acatcatttt
     1261 ccacaccaac aatctggtgt gttttatcat ctactccaac aatcagcttg ccaccttgtg
     1321 tattggcaaa agctacgatt gtcttcatgt acttttcact tttatcaggt aaggatacct
     1381 tgtactcaat atttttcgat tccccggaaa acatagtatc ttctaccatt ctttcactcc
     1441 tactcttcta agctatatca tcagctgtgt tatttctcca gcttcaacat aaatttatcc
     1501 gaatcattaa caatattata cgtttcatca ctgtcctccg gcaagtaaga aaatgaaaac
     1561 atctgctttc ctttctctgc ctgtcgcttt gatgaatata ccgtcacata taacgcattc
     1621 ggaatcgact gatcgtagct gaaccgcaca cttttaaatg aattttctct gcaacgtttc
     1681 acgatttctt ctgcacatac tttctggtcc gctacataca gtttatttac cactaccgtc
     1741 aaccgttctt catggtaatt gtccgctgtc attgcatagg aaccatacac atccatatca
     1801 cgcccatatt gcaaatatcc aagacaaccg cttataacca ttattatacc gattatcaga
     1861 ctgataaacc accttttttt catcttctcg ctcctttata cccctgtatt ataatacaag
     1921 tgtatgacag cacagcttat ttttcaatac agttgtattt ataattttag gaaaagacga
     1981 ggtgccgtta tgattgtata tgaatgatgt atcttataaa gaatggctgt cttttctcca
     2041 atgaacaggc agccatttct catgtaattc tttaatacaa gtctcccaca tatccatctg
     2101 cttccagatg catatagcac tcatccacct tctctgattc cagaatccgc aaaacgtgtt
     2161 ccatacctgt agcaagttct tttgttttta actcttccag cggaatccaa taaactcttc
     2221 cttcctcata actctttaat tctccggtaa acttatccgc tttatacaag gtaataacat
     2281 agtgcactcc gctcttatgc caatgatata atcctccaag cttcggtgct tcaaccgtaa
     2341 gtcctgtttc ttcccagact tcccgaatca tagatttctg aaaaatctca tttgcttcta
     2401 cgtgaccgcc tggaaaagtt gtaccggtat agctatcatt tactttatcc agagccagta
     2461 cattcccttt atgatcctga atcatacaca tattcataaa acagaccttc tcaatccgac
     2521 tctcttcttt ttcattcaga gattttctga cacgtttgtt gatatccgat cgtttggatt
     2581 tataatgctt gaagacctct ccatacggaa tccagatttc tgcatcttca attttttcat
     2641 aaacagaatc caccacttct tccagatgtt tgtcactcgg aaaagtacat tttttcatat
     2701 aataatctct ggttgcctga aacatccatt ccgcaatctt ttctttctgc ttcattttta
     2761 actgggaata cttcttattc gtttgcagta atctcccatc tatcatctta tggtttttcc
     2821 gtgacatcgg tttgacctct tttcttcttt ttcattcaga ttttactata ccacagaatc
     2881 atgcttgcgt catacttcag agcttcgatg tcctcttttt cttctctcag taccttattt
     2941 atagagggat cttttttaag gactctcttc cggaggctgt gccaccggta aatagcaagg
     3001 acaggaagtg tatatacact tccgaaaaac cagcaattta gggaaccctg ccctaaatta
     3061 aaagcccaga agtcactact tcgaagaacc aaagaaagga gaatctgcct atggcaaaga
     3121 gaaaaagaaa tatacagatt ttgttttgtg tttcccccga agaaaagaag cttatccgta
     3181 gaaagatgat tgaatcaaag accaaaaaca tgggagctta cggtgtattg ttcatattct
     3241 gttttcggga atctttatga aatcttcaaa attgcccggt acgctatccg taaaatcaaa
     3301 gaaaggactt gattatatgg atatgatttt gaaaacgact gacctctgca aaaatttcaa
     3361 ggggcaaatg gcggtaaaca atg
//
LOCUS       DBMDXG010000238         3380 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6365_length_3380_cov_1.608722, whole genome shotgun sequence.
ACCESSION   DBMDXG010000238 DBMDXG010000000
VERSION     DBMDXG010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6365_length_3380_cov_1.608722"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            181..930
                     /locus_tag="ACZPD3_07090"
     CDS             181..930
                     /locus_tag="ACZPD3_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR1415797.1"
                     /translation="MAEIRRKNRTTKVWAHRGASGYRPENTMEAFELAIKQGADGIEL
                     DVHTSADGELIVMHDETVDRVTDKTGLIKDMTLAQVKELKVSTDAEPNGIYRVPTLAE
                     VLDLMRTTDMMVNIELKNSICFYPGMEEKILKLVKEMHMEDQLIYSSFNHYSLMQLKQ
                     LDDHVQTGILFSDGWINPAMYAKNLGINAVHPAVYHLKYPQFMEEVKRAGLKMHVWTA
                     NKPEHIQLVKDADAEAVITNYPDRALEIVGK"
     gene            complement(1158..2639)
                     /locus_tag="ACZPD3_07095"
     CDS             complement(1158..2639)
                     /locus_tag="ACZPD3_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR1415798.1"
                     /translation="MKIGFDNDKYLKMQSEHIRERISKFGNKLYLEFGGKLFDDYHAS
                     RVLPGFEPDSKLRMLMQLSDQAEIVIVISAADIDKNKVRGDLGITYDEDVLRLMEVFT
                     ERGLYVGSVCITQYAGQESADAFKKRLEKLGIKVYVLYLIPGYPNNTSFIVSDEGYGK
                     NDYIETTRPLVVITAPGPGSGKMATCLSQLYHEYKRGVKAGYAKFETFPIWNIPLKHP
                     VNLAYEAATADLNDVNMIDPFHLEAYGETTINYNRDVEVFPVLQAMFEKIMGECPYKS
                     PTDMGVNMAGNCIVDDEVCKEAARQEIIRRYYKSMENLVRGTGREEEVYKIELLLKQA
                     HVNIEDRKVVPSALQREEETGAPAAAMELPDGRIITGKTSELLGASSALILNALKELA
                     GIDHDKHVISPEALRPIQTLKTEYLGSKNPRLHSDETLIALSISAADNEDAKLALQQI
                     PKLKGCQVHTSVLLSQVDILEFRKLGVELTCEPKSEHAKKLQK"
     gene            3144..>3380
                     /locus_tag="ACZPD3_07100"
     CDS             3144..>3380
                     /locus_tag="ACZPD3_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415799.1"
                     /translation="MSTKATGNKKHLTLADRAAIEHGISCGENFTQIAHRINKDSSTI
                     SKEIRRHLFRVPHFQNETQRKRSECEHYQNCEKQH"
BASE COUNT         1046 a          685 c          706 g          943 t
ORIGIN      
        1 tatataaaaa atgtgcataa tagcacagaa aagatccgta accggataaa aaaagaaccg
       61 cgggacacgc ggggatagcc tgttttagct ttgccctgaa gagcaatcga gcaggaagct
      121 cccacttcaa aatctgtaag atttaagtgg cgagagcatg tcacgagagg catatagaaa
      181 atggcagaga taagaaggaa aaacagaaca acaaaagtat gggcacacag aggtgccagc
      241 ggatacagac cagagaatac catggaagca tttgaacttg ccattaagca gggcgcagat
      301 ggaatcgaac tggatgtaca tacatcagca gatggcgaat tgatcgttat gcatgatgag
      361 accgtagatc gtgtcacaga taaaacagga ctgatcaaag atatgactct ggcacaggta
      421 aaagagttaa aggtcagcac agacgcagaa ccaaatggga tttaccgtgt tccaactctg
      481 gcagaagtat tggatctgat gcgcaccaca gatatgatgg taaatatcga attaaaaaac
      541 agcatctgct tttatccagg aatggaagag aagatattga aactggtaaa agaaatgcac
      601 atggaagatc agctgatcta ttcctcattc aaccattaca gtctgatgca gttaaaacaa
      661 ttggatgatc atgtacagac aggtattctt ttcagcgacg gatggatcaa tcctgccatg
      721 tatgccaaga atctgggaat caatgcggta catcctgctg tttatcatct gaaatatccg
      781 cagtttatgg aagaagtaaa acgtgcagga ttaaaaatgc acgtctggac agcaaacaaa
      841 ccggaacata tccagttagt aaaagatgca gacgctgaag cagtcattac caattatccg
      901 gacagagcat tggaaattgt aggaaaataa atcagataat tatcctccca atgtgctgtg
      961 aagtgctttg tatagagcgt gttaaaaaag ttcctctcat ttatatgaat aaatcatata
     1021 atcaaagagg aactttttgc atatcatatc attttatttt atatatcaat actataaagt
     1081 tattatgcgt ggatgataaa acttctgaaa tcgatatttc agtatttatg catgcgttga
     1141 tagtattttg tgcttagtta tttctgtaat ttcttagcat gttcagattt cggttcacag
     1201 gtaagctcga cacccagttt acggaattcc aggatatcta cctgagaaag caatacagaa
     1261 gtatgaacct ggcatccttt taacttcgga atctgctgaa gagccagttt cgcatcttca
     1321 ttgtcagcag cactgataga aagtgcaatc agagtctcat cagaatgaag tcttgggttc
     1381 ttgcttccaa ggtactctgt cttaagagtc tgaatcggtc ttaaagcctc cggagaaatg
     1441 acatgtttgt catgatcgat tccggcaagc tctttcaggg cattcagaat cagagctgaa
     1501 gatgcaccga ggagttcaga agtttttccg gtaatgatac gtccgtcagg aagttccata
     1561 gcagcagccg gagcacctgt ttcttcctca cgctgtaatg cagacggaac aactttgcgg
     1621 tcttctatat tgacatgtgc ctgtttcagc agaagctcga tcttataaac ttcttcttcg
     1681 cgtccggttc cgcgtaccag attttccatg ctcttataat atctgcggat gatctcctga
     1741 cgtgcagcct ctttgcagac ctcatcatcg acgatacaat ttcctgccat gttgacaccc
     1801 atatctgtcg gagatttgta agggcactca cccatgatct tctcaaacat agcctgaaga
     1861 accgggaaga cttctacatc acgattgtag ttaatggtag tttcgccgta tgcttccaga
     1921 tggaatggat cgatcatgtt gacatcgttt aaatctgcag ttgcagcttc ataagctaag
     1981 tttaccggat gtttcagcgg aatattccag attgggaaag tttcaaattt cgcataacct
     2041 gctttgacac cacgtttgta ttcgtgataa agctgggaaa ggcaggtagc catttttccg
     2101 cttcctggtc ccggggcagt aatgacaacc agcggtcttg ttgtttcaat atagtcattc
     2161 ttaccatatc cttcgtcact tacgataaag gaagtattat ttggataacc aggaatcaga
     2221 taaagcacat aaactttgat tccaagcttt tcaagacgtt tcttgaatgc gtctgcactt
     2281 tcctgtccgg catactgagt gatgcagaca cttcctacat aaagaccgcg ctctgtgaat
     2341 acttccataa ggcggagtac atcttcatca taagtgatac caaggtcacc tctgacttta
     2401 tttttatcaa tgtcagctgc gctgattacg atgacaatct ctgcctgatc agaaagctgc
     2461 atcagcatgc gcaatttact gtccggctca aatcccggaa gaactctgga agcatggtag
     2521 tcatcaaaaa gcttgccgcc aaattccaga taaagcttgt taccaaactt gctgatacgt
     2581 tcccgaatgt gttcggactg cattttcaga tacttgtcgt tgtcaaatcc tatcttcatt
     2641 tgttcatttc ctttcccatc acataattag tcacttatta ttactgctcg tggttggtat
     2701 cccgcgaggt gctttggcat attatgccaa aatcccgaga catacagcgc gaatttatgc
     2761 ggttagcata aattctgtgt atcgcgaagc gtattactga gaacgaagtg agcagtaatc
     2821 actttgttaa tagtatacta caaagagagc aatttgtaac aggtaaattt gtaaaagtga
     2881 aaaaaattta ttatggaatt acacatgggg agaatgataa aaaaataaaa atagattgac
     2941 ggataagagg aatctggtgt aaaattttga ccctaaaatg aaaaactaac ttccaccctt
     3001 agcctatatt gttctacact gtgaaatagg ggtggaagtt agtcttgcaa tcgtttatcc
     3061 tataagaaat accagggctc cccctggtca tatcctccaa ctaccggaaa tggaatattt
     3121 ttattacata ggaggattca tttatgtcta cgaaagctac tggaaacaaa aaacacctta
     3181 ccctcgctga tcgtgctgcc attgaacatg gaatcagctg tggggaaaat tttactcaga
     3241 ttgcccacag gatcaacaag gattcttcca ccatatccaa ggagattcga cggcatcttt
     3301 tccgggttcc tcattttcaa aatgaaaccc agcggaaacg ctcggaatgt gagcattatc
     3361 agaactgtga aaaacagcat
//
LOCUS       DBMDXG010000239         3366 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6397_length_3366_cov_1.930168, whole genome shotgun sequence.
ACCESSION   DBMDXG010000239 DBMDXG010000000
VERSION     DBMDXG010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6397_length_3366_cov_1.930168"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2475)
                     /locus_tag="ACZPD3_07105"
     CDS             complement(<1..2475)
                     /locus_tag="ACZPD3_07105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1415800.1"
                     /translation="MSRQIRRQIPQKQKKKRVDRNACLLVFILIFSGILTFLLLTARD
                     NSKLNSTLDETFDFVKNRIESYEIYNTNDQVKSLVRLLDKTTELSRVIEQEGNLSTEM
                     LDEYAREQRLTGILVLDQNLKVTEQSAKDGDTMSLWQKLIKSDYVRDIAEHPEKTYTT
                     RLRNKGKIYDFAAVARQDAAGILITYAQKEEVNELNGDLTMASLFSDFPFEMNGSVVI
                     CDDDKVVSTNRQELTARSIEEAKSQYKNKFEVDENGIVHLRSETGNWFGRREKIKDYD
                     AYIFFPESQVYITRNIICVIYVLLALLLFLLYRVNRSRTEKRSILQDQKRLRVINALG
                     HAYASISLVNLKTEEIEILKSSGNMKPDQKGDMLFKAHQEELIRQVIAEPFQKAYWEF
                     VDISTVAKRLEERETLSFTARTVDERWMTMIIVPQGYDKDGKLCTVLVAIRDATEEKE
                     REIAQDRNLRNALAAAEHANRAKTVFLNNMSHDIRTPMNAVIGFTALATTHIDNKELV
                     LDYLKKIHISGQHLLSLINDVLDMSRIENGSVRIEYTVVHLPDILHDLNTIIQESVHS
                     KQQELYIDTQDVLHEDIITDKLRLTQVLLNISSNAVKFTPVGGTVNIRVSEKPCRRDG
                     CTTVVFSVKDNGIGMSPEFREQVFDSFTREHTVTENGIGGTSLGMAITKNIVDMLGGT
                     IQVESEVGRGTEFTVMLECEISGTTVKEEPDSEQREPLKNEKQKIRAEIQRRYEGKKV
                     LLVEDNELNREIATAIMEEIGLDVDCVEDGTDAVNIMSSAEGRKYDLIFMDIQMPKMD
                     GYTATREIRTLNDPKCANIPIIAMTAN"
     gene            complement(2472..3311)
                     /locus_tag="ACZPD3_07110"
     CDS             complement(2472..3311)
                     /locus_tag="ACZPD3_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR1415801.1"
                     /translation="MRKNRKNDSHTRRKRICIAALLLAGSLIPGGCGIKKEKTSDSEL
                     PEIVIGIDYFEPYSYQTSDGEYKGIDVELAEEAFQRLGYQPEFEKVVWEDKEKLLSDG
                     TIDCLWSCYSMNERENKYQWAGPYMYSRQMVVVRAESEIRTLQDLKGKRIAVQATTKA
                     EDLFLHNIKSDLPHAEQVNCFSSSNELYAALRKNYADAIAGHEAMLGSLIQDGKGVYR
                     MLEESPYKSELGIAFKKGTHEELAEKLTETLTEMQSEGITEKIVTKYGLDADETLPGG
                     ESK"
BASE COUNT          808 a          883 c          558 g         1117 t
ORIGIN      
        1 attggcagtc atcgcaatga ttggtatatt tgcgcatttc ggatcattca atgtacggat
       61 ttcccgggtt gcggtgtatc catccatctt cggcatctga atatccatga agatcagatc
      121 atattttctg ccttctgcag atgacatgat attcacagca tctgtaccat cctcaacgca
      181 atccacatcc agaccaattt cctccatgat cgctgttgcg atctcccggt tcagctcatt
      241 atcctctaca agaagaactt tcttcccttc ataacgcctc tggatttctg cccggatttt
      301 ctgcttctca ttcttaaggg gctctctttg ctccgaatca ggttcctcct tcacagtcgt
      361 ccctgatatt tcacattcaa gcataaccgt aaactccgtt ccccttccta cttcgctttc
      421 aacctgaatc gtaccgccca gcatatccac aatgtttttg gtgattgcca ttccgagact
      481 ggttcctcct attccattct ccgtaacggt atgttctctt gtaaatgagt caaacacctg
      541 ctccctgaac tccggcgaca tcccaattcc attatccttc acactgaata ccactgttgt
      601 acatccgtct cttctgcatg gtttctcaga aacacgaatg ttaaccgtac ccccgaccgg
      661 tgtaaatttc acagcattac tgctgatatt cagaagcacc tgcgtcaggc gcaacttgtc
      721 ggtaatgata tcttcatgga gtacatcctg tgtatcaata tacagctcct gctgtttaga
      781 atgtacagat tcctgtataa ttgtatttaa gtcatgcaag atatccggca ggtgtacaac
      841 tgtatattca atccgtacgg aaccgttctc gattctgctc atatccagca catcattgat
      901 taggctgagc agatgctgac cggatatatg gatttttttc agataatcca gaacaagctc
      961 cttattgtca atatgtgttg tcgcaagcgc agtaaatcca atgaccgcat tcatcggcgt
     1021 gcggatatca tgagacatat tattcagaaa cacagtcttc gccctgttcg catgttctgc
     1081 agccgcaagt gcatttctca gattcctatc ctgcgcaatc tcacgttctt tttcttctgt
     1141 cgcatcacgg attgcaacca gaactgtaca caatttccca tctttatcat atccctgtgg
     1201 cacaatgatc attgtcatcc atctttcatc cactgtccga gctgtaaaac tcagcgtttc
     1261 acgttcttcc agacgttttg ccaccgtact tatatcgaca aattcccagt atgccttctg
     1321 aaatggctct gcaatcacct gccttataag ttcttcctga tgcgctttga aaagcatatc
     1381 ccctttctga tctggcttca tatttcctga agatttcagg atttcgattt cctctgtttt
     1441 tagattcaca agagaaatag aggcatatgc atgccccagt gcattgatca cccgaagtct
     1501 tttctggtcc tgaaggatac ttctcttttc tgttctgctc ctgttcaccc gataaagtaa
     1561 gaaaaggagc agtgcaagca gcacataaat cacacatata atatttcgtg tgatgtacac
     1621 ctgactttcc gggaaaaaga tatatgcatc gtagtctttg atcttctccc gacgtccgaa
     1681 ccagtttcct gtctctgaac gaagatggac aattccattc tcatctacct caaatttatt
     1741 tttatactga ctttttgctt cttctataga gcgggcagtc agctcctgtc tgtttgtact
     1801 gacaacctta tcatcatcac agatcacaac acttccattc atctcaaaag gaaagtcaga
     1861 aaataacgat gccatcgtca gatctccgtt aagctcattt acttcctcct tctgcgcata
     1921 tgtgatcagg atacctgctg catcctgtct tgccactgcc gcaaaatcat agatctttcc
     1981 tttatttctc agacgagtgg tataagtctt ttccggatgc tctgcaatat cacgtacata
     2041 atcacttttg atcagcttct gccacagcga catagtatct ccatcttttg cactctgttc
     2101 tgtcaccttc agattctgat ccagtacaag gattcctgtc aggcgctgtt ccctggcata
     2161 ttcgtctagc atttctgtac tgagatttcc ttcctgttct atgactctgc ttaattctgt
     2221 tgttttgtcc aaaagacgta ccaggctttt cacctgatca ttggtgttat aaatctcata
     2281 actttcaatt cggtttttta caaaatcaaa ggtctcatcc agagtactgt tcagcttgct
     2341 attatctctc gctgtcagca gaagaaaagt cagtattccg gaaaaaatca gaataaacac
     2401 aagcaaacac gcatttctgt cgaccctctt tttcttctgt ttctgcggta tctgccttct
     2461 tatctgtctg ctcatttact ttctcctccc ggcagagttt cgtccgcatc caatccgtat
     2521 tttgtaacta ttttttctgt aatcccttcg ctttgcatct ctgtaagcgt ttctgtaagc
     2581 ttttctgcca attcttcgtg agttcctttt ttgaaagcaa ttccaagttc tgatttataa
     2641 ggactttcct ccagcatacg gtaaacgccc ttgccatcct gtatcagact gccaagcatt
     2701 gcctcatgtc ccgcaatcgc atctgcataa ttttttctaa gtgcagcata aagctcattc
     2761 gacgaagaaa aacagttcac ctgttctgca tgcggaagat ctgattttat attatgaaga
     2821 aagagatctt ctgcttttgt agtcgcctga actgcaatcc tcttcccttt cagatcctga
     2881 agtgtccgga tttcactctc ggctcttaca accaccatct gacgactgta catatacggt
     2941 cctgcccact gatatttgtt ttctctttca ttcatggaat agcagctcca cagacagtct
     3001 attgttccat ctgacaaaag tttctcttta tcttcccaga caaccttctc aaattcaggc
     3061 tgataaccca gcctctgaaa ggcttcctct gcaagttcca catcaatacc tttatactcg
     3121 ccatcactcg tctgatagct gtaaggttca aaataatcta ttccgataac aatctccggc
     3181 aattcactgt ccgatgtttt ctctttcttt attccacatc ctcccggtat aaggctgcct
     3241 gccagaagaa gtgctgcaat acaaattctt tttctgcggg tatgggagtc attctttcga
     3301 ttttttctca tatccagttc ctttccattt attactgttt cataacctgc gtgtcagtga
     3361 ccttgt
//
LOCUS       DBMDXG010000240         3362 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6407_length_3362_cov_1.848206, whole genome shotgun sequence.
ACCESSION   DBMDXG010000240 DBMDXG010000000
VERSION     DBMDXG010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6407_length_3362_cov_1.848206"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="ACZPD3_07115"
     CDS             <1..1026
                     /locus_tag="ACZPD3_07115"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR1415802.1"
                     /translation="IGAAYPNNSSVIRITSEGYDKEVVVDINDLASVPKAQGTVSLVA
                     GMVEAIQKFGFKVAGFDAYVTTNVIRAAGVSSSASFEMLVCSIINYFFNDGAMTYINY
                     AKAGQYAENVYWLKASGLMDQLACAVGGPILLDFSDRENPKYEKVNFSFHDYDHHLVI
                     VNTGKGHADLSEEYSEIPMEMKEAAKAAGAELLCETTLDKVLANMDKIDNDRAILRAI
                     HFFKENERVERAAKAVEEKDGETVLKLLSESGESSWELLQNCYPIKAYTEQKISVALA
                     LTDLFLEKLGKGICRIHGGGFAGVIMCVVPEEETENYVSYISEFAGKENVYPMNIRAV
                     GAVHIEK"
     gene            1439..3244
                     /locus_tag="ACZPD3_07120"
     CDS             1439..3244
                     /locus_tag="ACZPD3_07120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415803.1"
                     /translation="MKKRWTMRRALLVLCIASVVFALLLQTFLFHQSLRRQIRAESIS
                     DHEISLNKMQTDLSNFIHTIRGEMLTIYSEQDLIAAMREAAEKDTDMKDYYWRSWYFA
                     RKRFTKEDQLLAMYLYDTKDHLISSYRYNSVTYPKDIYKAEYDDNSERVYDYVHGKRT
                     DLMISGYHNSAAKKDIVRFVLKLHNYDADRNALGYLVCDINSAALTSIMAKYVDVEQV
                     CLWLQPLNDRVIAMTGEASESQCRIQKQLAKVVQSHYKSGELEQEYDGNYLIQVSQEN
                     YNLEAFVLVSQSLLTATQKTLIRTLLIIMGAMILAIMVIVLFVSQWMTKPVEEMSSTI
                     TRIKDGETQLRVQLVGWSQELTTLGTEFNEMLDRMQVMAQEELQHKMLVERTEYKMLQ
                     AQINPHFLYNTLDTMSGIANAQNCPMVSGMCHSLSAIFRYSLNMTDELSTVQNEMAHV
                     RNYLYVMDVRNGSTIAYDYQIDSDTLADQMPRICIQPVVENALTHGLRNVRRKDKKLL
                     IRSEHVNENLVITIQDNGAGMDAESMNRLLDQNDMKRVESGISIGILNVNARLKRLFG
                     EKYGLHIESTAGEGTTVTITVPAVSTENSGDMENV"
BASE COUNT         1005 a          606 c          832 g          919 t
ORIGIN      
        1 atcggtgcag cttacccgaa caacagcagt gttatccgta tcacaagtga aggatatgat
       61 aaggaagttg tcgtagacat caatgatctt gccagcgtac cgaaggctca gggaaccgta
      121 tctctggtag caggtatggt ggaagctatt cagaaattcg gttttaaagt agcgggtttt
      181 gatgcttatg tgaccacaaa tgtaatccgc gcagcaggtg taagttcttc tgcatctttc
      241 gaaatgctgg tatgttccat cattaattat ttctttaatg atggtgcaat gacatacatc
      301 aactatgcaa aagcaggaca gtatgctgag aatgtttact ggctgaaggc ttccggatta
      361 atggatcagc ttgcatgtgc agttggcgga ccgatccttc tggacttctc agacagagag
      421 aatccaaaat atgagaaagt aaacttctct ttccatgatt atgatcatca tcttgtaatc
      481 gttaatacag gaaaaggtca tgctgattta agtgaagaat actctgagat ccctatggaa
      541 atgaaagaag cagccaaggc agcaggagca gagcttctct gtgagacaac tcttgacaaa
      601 gtgcttgcaa atatggataa gatcgataat gacagagcaa tcttaagagc gattcatttc
      661 ttcaaagaga acgaacgtgt agagagagct gcaaaagcag ttgaagagaa agatggtgag
      721 actgtactga agctcctcag tgaatcagga gaatcttcct gggaattact tcagaactgc
      781 tatccgatca aagcatatac agagcagaag atttctgttg cgcttgctct tacagatctc
      841 ttccttgaga aactgggcaa aggtatctgt cgtatccatg gaggcggatt tgccggtgtt
      901 atcatgtgcg tagtgccgga agaagaaaca gagaactatg tttcctatat ttctgaattt
      961 gccggaaaag agaacgtata tccaatgaat atccgcgcag taggtgcagt acatattgaa
     1021 aagtaaatat gccgtaaaag gtgttactga acataaaatt atgagcagcc agcgaagcgg
     1081 attgtgaata tctgtttgac aaatattgag ttaaatgtta tcaggaaaaa caggcagcag
     1141 taaggtggat tggattgaca tacactttac tgctgttttt ttgatggaag tttcctgttt
     1201 tgcttttcaa tgcaaaattc ctgtattata attgtgataa tgtataatta tgataaggat
     1261 gtatggtgat ttttgtagat aatgcatttt gtatctgttt atcagaatgt gaaatttaca
     1321 ggaattgcta cagaattatc aggtatctaa ccgaataaac tgcggagcag ttattcgatg
     1381 tagagccgca taacaaagcg gatgattttt atccgcttga catgaggggg aaggaataat
     1441 gaaaaaaagg tggacaatgc ggagggcact gcttgttctg tgcattgcaa gtgtagtgtt
     1501 cgcacttctt ctgcagacat ttctgtttca tcagtctctg cgcagacaga ttcgggcaga
     1561 gagcatttca gatcatgaga tttctctgaa taaaatgcag acagatctgt ctaattttat
     1621 ccacacaata cgaggggaaa tgcttacgat ctacagtgag caggatctta tcgcagctat
     1681 gcgtgaagct gcggaaaaag atacagacat gaaggattat tactggagaa gctggtattt
     1741 tgcaaggaag cgttttacaa aggaggatca gcttcttgcc atgtatcttt atgatacaaa
     1801 ggatcatctg atcagcagct accgctacaa ttcagttaca tatcccaaag atatttacaa
     1861 ggcagagtat gatgataatt cagaacgggt ttatgattat gtgcatggaa aacgtacaga
     1921 tctgatgatt tccggatatc acaattctgc cgcaaagaaa gatattgtgc gatttgtgct
     1981 gaaactgcat aattatgatg cagacaggaa tgccctgggg tatctggtct gtgatattaa
     2041 cagtgcggca cttacttcta ttatggctaa atatgtagat gtagagcagg tatgtctgtg
     2101 gctgcagcca ttaaatgacc gggtgatcgc aatgaccggg gaggcttcag agagtcagtg
     2161 cagaatccag aaacagcttg caaaggttgt acaaagtcat tataaatccg gtgaactgga
     2221 acaggagtat gatgggaatt atctgatcca ggtgtctcag gagaattata atctggaggc
     2281 atttgtgctt gtctcacagt ctcttctcac tgcaacgcag aagacactga tccgaacact
     2341 tcttattata atgggagcca tgatccttgc aattatggtg atcgtattgt ttgtgtctca
     2401 gtggatgaca aagcctgtag aagagatgag cagcactatc acgcggatca aggacggaga
     2461 aacccagctt cgggtgcagc tcgtaggatg gtcccaggag ctgactacgc tgggtacgga
     2521 atttaatgaa atgcttgacc gtatgcaggt catggcgcag gaagaactgc agcataaaat
     2581 gctggtagag cgtacggaat acaaaatgct gcaggcgcag atcaatcccc atttcctcta
     2641 taatacactg gataccatga gcgggattgc caatgctcag aactgtccga tggtaagcgg
     2701 tatgtgtcat tcactgtctg caattttccg ctacagtctg aatatgacag atgagctttc
     2761 cactgtgcag aatgagatgg cgcatgtgag aaattatctc tatgttatgg atgtgagaaa
     2821 tggctcaacg atagcttacg attatcagat tgacagcgat acactggcag atcagatgcc
     2881 gcgtatctgt atccagccgg ttgtggagaa tgcacttaca catggtctca gaaacgtccg
     2941 ccgtaaggac aaaaagcttc tgatccgttc agaacatgtg aatgagaatc tggtgatcac
     3001 aattcaggat aatggtgcgg gaatggatgc ggaatctatg aaccgtctgc tggatcagaa
     3061 tgatatgaaa cgtgtggaat ccggcatttc cattggaatc ctgaatgtta atgcgcgtct
     3121 gaagaggctt ttcggagaga aatacggact gcatattgaa agtacagcag gcgaggggac
     3181 aactgtgacg atcacagtcc cggctgtttc tacggagaat tccggtgata tggaaaatgt
     3241 gtaactgttc agtaccttca cagttacgag cccaaatgca ttaaaaatca cctgcggtga
     3301 tggcattttg gcttgtatgt ctcgggattt tgggacttac gtgccaaaac acctcgcggg
     3361 at
//
LOCUS       DBMDXG010000241         3360 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6415_length_3360_cov_1.744400, whole genome shotgun sequence.
ACCESSION   DBMDXG010000241 DBMDXG010000000
VERSION     DBMDXG010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6415_length_3360_cov_1.744400"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="ACZPD3_07125"
     CDS             <1..556
                     /locus_tag="ACZPD3_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1415804.1"
                     /translation="GTAGVTVGLALQGGLANIAGGIMLLIFKPFQVGDYVIIAQQNGC
                     EGTVYKIEICYTTLISIDNKHIVIPNGTLSGSIITNVTARDLRKLEIKVGISYDSDIK
                     KAKAILEEILHNDEDTKDDQGMVVFVDELGESAVVMGLRVWVATEKYWPARWRLNELI
                     KEAFDANGIEIPYNQLQVHVKKQI"
     gene            948..2558
                     /locus_tag="ACZPD3_07130"
     CDS             948..2558
                     /locus_tag="ACZPD3_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1415805.1"
                     /translation="MKMYKKVMAAALAGVMTVSMSATAFAADDDGYILKATNEDSKSS
                     DNDLVIAIEGSVSSMDPANIPDTNAISATRGVYETLVSFDENQELTGKLAESWEVSDD
                     SLTYTFKLRQGVKFQDGTDFNAAAVKANYDRVVNKDNNLRQRRTFIVTNEDGSETPRV
                     DSIETPDDYTLVIKLTQAWAPFINRLTQFCIISPAALEEYGNDIMNHPCGTGPYVCTE
                     WEEGDHTSFKRNDDYWGDKPGVDTVTIKEVPEAGARTAMLQTGEADFIYPTPSDQIEA
                     IKGTDDVNILTTDSNIMRYVTLNMDLEQLSDVKVRQAMNYAIDKDAYIQLMYSGYGKP
                     ATSVVPSIIGGYEEQEAYTYDVDKAKELMKEAGYEDGFKLTLWGDNTTQEIKGMTFIS
                     QQLAQIGIEVDVQPMEPATVSDKIYVDKEDAEINMWYVNWSASDFTMDGSLRSLLYST
                     MCPPTSANTAYFNDADFDKDMDEGLATANEEEQAKYYSDAQKIAWEACPWLFLGNDQI
                     IYSTKSYLSGVYVSPDGAFNFANATLAQ"
     gene            2772..>3360
                     /locus_tag="ACZPD3_07135"
     CDS             2772..>3360
                     /locus_tag="ACZPD3_07135"
                     /inference="COORDINATES: protein motif:HMM:NF040248.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1415806.1"
                     /translation="MGRYLGKRLISTIPLLLVISFVVFMFIHMIPGDPARLVAGQDAT
                     KEDVAVVREQLGLDEPLLVQYGKYMKGLFTGDLGSSIKNGKTVTETIAPRLKPTIMLT
                     FSSMIWAAIIGIAIGIIAAVFHGRILDYVGMIIAIAGISVPSFWLGLELIQLFSVSLG
                     WLPTSGLETWKSYILPSLTMGAGIMAVLARFSRSSM"
BASE COUNT         1112 a          653 c          738 g          857 t
ORIGIN      
        1 tggtactgct ggtgtaacgg taggtcttgc attgcagggc ggtcttgcaa atatcgcggg
       61 cgggatcatg cttctgatct ttaaaccatt tcaagtgggg gattatgtca ttatcgcaca
      121 gcagaatggg tgcgaaggaa ctgtatacaa aatagaaatc tgttatacaa ctctgatttc
      181 catagataac aaacacattg taatcccaaa tggaacgctg tccggcagca ttattacaaa
      241 tgtaactgcc agagatcttc gtaagctgga gatcaaagta ggaatttctt atgattcaga
      301 cattaagaaa gcaaaagcaa tcctggaaga aatccttcat aatgatgaag atacgaaaga
      361 cgaccaggga atggtggtgt ttgtagatga gcttggagag agcgcagtgg tcatgggcct
      421 gcgtgtgtgg gtggctacgg agaaatactg gcctgccagg tggcggctta atgaactgat
      481 caaagaagcg tttgatgcaa acggaattga aatcccgtac aatcagctgc aggttcatgt
      541 gaagaaacag atatgataac aacataagtc ctctgaacca actataaggt ttagaggact
      601 tattgtttgt acagattata gccaaataat accaaacaag aataaataat ccttaattta
      661 tagtaattta taccaatttc attgtcagat attggtataa taccaccaaa ataatataaa
      721 atacgacaaa ataattgaca ataggacaac agattgctat actggagtca agttaaacaa
      781 ctaagtgttt ttatgaagat gcaaaggcgc atgtaagtaa atggattgct gcacgatttt
      841 tatacattgc aaagacgcaa aaatataaaa acactttttt tgtacccaaa attaacttcg
      901 tgattgtaac taagtaacaa tgtacagaaa gaaaaggaga gaaaactatg aaaatgtaca
      961 aaaaagttat ggcagcagct cttgcaggag taatgacagt ttccatgtcc gctacagcat
     1021 ttgcagcaga tgatgacggc tatattctca aagccacaaa tgaagattcc aaatcatccg
     1081 acaatgatct ggtgatcgca atcgaaggaa gcgtaagctc catggatccg gcaaacattc
     1141 cggatacaaa cgcaatctct gctacacgtg gtgtatacga gacactggtt tcttttgatg
     1201 agaaccagga attaacaggc aaacttgctg aatcatggga agtttccgat gactctctta
     1261 cttatacttt caaacttcgt cagggcgtta aattccagga cggaacagac ttcaatgcag
     1321 cagcagttaa agcaaactac gatcgtgtag ttaacaaaga caacaacctt cgtcaaagaa
     1381 gaacatttat cgtaactaac gaagacggat ctgaaacacc tcgtgtagac agcattgaaa
     1441 caccggatga ttacacactg gttatcaaac tgacacaggc atgggcacca tttatcaacc
     1501 gtctgacaca gttctgtatc atcagccctg cagctcttga agagtatggc aatgatatca
     1561 tgaaccatcc atgcggaacc ggcccatatg tatgtacaga atgggaagag ggagaccaca
     1621 caagctttaa gcgcaatgat gattattggg gagacaaacc gggcgttgac actgtaacaa
     1681 tcaaagaggt tccggaagca ggtgcacgta cagctatgct tcagacaggt gaagcagact
     1741 tcatctatcc tacaccgtct gaccagatcg aagcaatcaa aggaacagac gatgtaaata
     1801 tccttacaac agactctaac atcatgagat atgtaactct gaacatggat ctggaacagc
     1861 tttctgatgt aaaagtacgt caggctatga actatgcgat tgacaaagat gcatacatcc
     1921 agttaatgta ttccggatac ggaaaaccgg ctacatctgt agttccatct atcatcggcg
     1981 gatatgaaga gcaggaagct tacacatatg atgtagacaa agcaaaagaa cttatgaaag
     2041 aagccggata cgaagacgga ttcaaactta ctctgtgggg cgataacaca acacaggaaa
     2101 tcaaaggtat gacatttatt tcccagcagc ttgctcagat cggtatcgaa gttgacgttc
     2161 agccaatgga gcctgcaact gtaagtgaca agatttatgt tgacaaagaa gatgctgaga
     2221 tcaatatgtg gtatgtaaac tggtctgcat ctgacttcac aatggatggt tctttacgtt
     2281 ccctgcttta cagcacaatg tgccctccta caagtgcaaa cacagcatat ttcaacgatg
     2341 ctgattttga taaagatatg gatgaaggtc ttgcaacagc taacgaagaa gaacaggcta
     2401 aatactatag cgatgctcag aagatcgcat gggaagcatg cccgtggtta ttccttggca
     2461 atgaccagat catctattct acaaaatctt atctgtctgg cgtatatgta tctccggacg
     2521 gagcatttaa cttcgcaaat gcaactttag cacagtaagt aatttgtttg agaacaggct
     2581 gcgaagaaca taaatctggc tgcctgatct gaaattgcag caattccata aaaatactgc
     2641 cgatacagca gtgtgaatac aggacaaaga tgttcaaacc gaagaactgc cataagattc
     2701 actatggcag ttctccgact ataaagcatc aaatataaac aacaggtaag aaaatgataa
     2761 ggaggaaaaa tatgggacgt tatttaggta aacgtttgat aagcacgatc ccgctgcttc
     2821 tggttatctc attcgtagta tttatgttta tccatatgat tcccggtgat ccggcaagac
     2881 tggtagccgg tcaggatgca acaaaggaag atgtggcagt tgtacgtgaa cagctgggac
     2941 tggatgagcc actacttgta cagtatggca aatatatgaa aggtcttttt acaggagatc
     3001 tgggaagctc tatcaaaaac ggtaaaaccg taacagagac aatcgcacca agactgaaac
     3061 caactatcat gctgacattc tcatccatga tatgggctgc gatcatcggt attgcgattg
     3121 gtattatcgc tgccgtattt catggcagaa ttcttgatta tgtaggtatg atcatagcaa
     3181 tcgcaggtat atctgttcca agtttctggt taggtctgga attaatccag cttttctcag
     3241 taagcctggg atggcttccg accagtggac tggaaacctg gaagagctat attcttccat
     3301 cacttacaat gggagcagga atcatggccg tacttgccag attttccaga tcttccatgc
//
LOCUS       DBMDXG010000242         3358 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6418_length_3358_cov_1.470986, whole genome shotgun sequence.
ACCESSION   DBMDXG010000242 DBMDXG010000000
VERSION     DBMDXG010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6418_length_3358_cov_1.470986"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="ACZPD3_07140"
     CDS             complement(<1..1410)
                     /locus_tag="ACZPD3_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR1415807.1"
                     /translation="MNRQAVQTLKTYFGYDTFREGQESVVESILEHRDVLAIMPTGAG
                     KSICYQVPALMLSGITIVISPLISLMQDQVKALNEAGIHAAFINSSLSESQISKALYL
                     AAGGRYKIIYVAPERLENYEFLEFARQVEISMVTVDEAHCISQWGQDFRPSYVKIVDF
                     VKNLPGRPIVSAFTATATEEVKNDILCTLNLEDPKVVITGFDRKNLYYSVENIRRKDD
                     FVMDYIDRHPTESGIIYCSTRKNVDNLFELLFQKGVAVTRYHAGLNNETRKKNQDDFI
                     YDRTPVIIATNAFGMGIDKSNVRYVIHYNMPQSMENYYQEAGRAGRDGENSQCVLLFS
                     AQDVMIDRMLLDNKDFSDVDEEDEFLIRQRDIRRLQIMEGYCKTTGCLRNYILEYFGE
                     KTFGPCDNCGNCHREYHETDMTREAKWVVNCVAETRGRYGLTIVLGTLLGAKRARLRE
                     LGADKYKSYGALNDHSEAEL"
     gene            complement(1436..2323)
                     /locus_tag="ACZPD3_07145"
     CDS             complement(1436..2323)
                     /locus_tag="ACZPD3_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR1415808.1"
                     /translation="MICGMTYFQICLYFLIYSFGGWVVEVIFHAVALGKVINRGFLNG
                     PVCPVYGFGVLSVFALLNTIQGSGRQMSDGMIFVFGIVLATAVELVAGWLLDVCFHAR
                     WWDYSDKPFNFHGYICLEFSLIWGLAIVMVVKVFQKYVEAHALHTPATWEWIVIAVLY
                     AVYLTDFIVTVAVIQGLNKKLTRLDKVRSDLRIVSDKLSDTLATTTIGTAQKVGEGKV
                     QATLAAAELRDATAAQREKSIEMLRIKRAELQAQFEELSSSITNHTVVGQGRIIKAFP
                     KMQHRDYSELIQELKKRLK"
     gene            complement(2391..>3358)
                     /locus_tag="ACZPD3_07150"
     CDS             complement(2391..>3358)
                     /locus_tag="ACZPD3_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1415809.1"
                     /translation="GTGLGMAITKNIVDMMGGTIEVQTEQGKGTEFIIRLPLRIQPEN
                     HRIEKIAELEGLKALVVDDDFNTCDSVTKMLVKVGMRSEWTLSGKEAVLRARQSMEMG
                     DAFHAYIIDWRLPDMNGIEVTRQIRSLGDDTPIIILTAYDWSDIEVEARAAGVTAFCA
                     KPMFMSDIRETLMAAIGQKQAGAEDNILPAADSDFRGRQILLVEDNELNREIAVALLS
                     EYGFQVDTAEDGAEAVEKVKNSRPGDYDLVLMDVQMPVMNGYEATEQIRSLDDPALAG
                     ITILAMTANAFDEDRKKALACGMDGFLSKPIVIEELISTLQNSLG"
BASE COUNT          905 a          910 c          569 g          974 t
ORIGIN      
        1 gagttctgcc tcactgtgat cattcaatgc tccataagat ttatacttat ctgctccaag
       61 ctccctcaac cgggctctct tagcccccag cagagtacca agcacaattg tcagtccata
      121 ccttcctctc gtctcagcca cacagttcac tacccacttc gcctctctgg tcatatctgt
      181 ctcatggtat tctctgtgac aatttccaca attatcacag ggaccgaagg tcttttcacc
      241 gaaatactcc agaatataat ttcgcagaca tcctgttgtt ttacaatatc cttccatgat
      301 ctgaagcctt cgtatatccc gctgacgaat gagaaattca tcctcttcat ccacatctga
      361 aaaatctttg ttatccagaa gcattcgatc gatcataaca tcctgtgcag aaaacaatag
      421 aacgcactgg gaattctctc cgtcacgccc tgcacgtcct gcctcctgat aataattctc
      481 catactctgc ggcatattgt aatggatcac atatctcaca ttggatttat caatccccat
      541 tccaaatgca tttgttgcaa taataacagg agttctgtca taaatgaaat catcctgatt
      601 cttttttctg gtttcattat tcagtcctgc atgatatctg gtaacagcaa ctcctttttg
      661 gaaaagcagt tcaaaaagat tgtccacatt ttttcttgta gagcagtaaa taatcccgct
      721 ctctgttgga tgtctgtcta tgtaatccat cacaaaatcg tcttttcgcc ggatattttc
      781 cacgctgtaa tataaattct ttctgtcaaa cccggtgatc actactttcg gatcttccag
      841 atttaaagta cagagaatgt cgttctttac ttcctctgtt gcagttgcgg taaaagcact
      901 gacgatcgga cgaccgggaa gattttttac aaagtctacg attttgacat agcttggtct
      961 gaaatcctgg ccccactgtg aaatacaatg tgcctcatcc actgtcacca tggaaatctc
     1021 cacctgtctg gcaaattcca gaaactcata attttccaga cgttccggtg caacataaat
     1081 aattttgtat cttcctcctg ctgccaggta caacgccttg ctaatctgac tttccgacag
     1141 tgaagaattg ataaatgcgg catgtattcc cgcttcattc aaagccttta cctgatcctg
     1201 catcagtgag atcagaggag aaattacaat cgtaattcct gacagcatca gtgccggtac
     1261 ctgataacag atggattttc cggctcctgt cggcatgatt gcaagcacat cccggtgttc
     1321 cagaatggac tccacaacac tctcctgccc ttctctgaat gtatcatatc caaaataagt
     1381 ctttaaggtc tgtactgcct gtctgttcac ctgaagcctc cctctgcatt ttaatctatt
     1441 ttaatctctt tttcagctcc tgaatcagtt cggaatagtc tctgtgctgc atcttcggaa
     1501 atgccttgat gatacgcccc tgaccgacaa cggtatgatt ggtaatagag ctggacagtt
     1561 cctcaaactg agcctgaagc tccgctcttt ttatacgaag catttcgata gatttttctc
     1621 gctgtgcggc agttgcatca cgcagttctg ctgctgcaag agttgcctga acctttcctt
     1681 ctcccacttt ctgtgctgtg ccaatcgtag tcgtagccag tgtatcactc agtttatcac
     1741 ttacaattct caaatctgag cgaaccttat ccagtctggt aagcttttta ttcagtccct
     1801 ggatcactgc taccgtcaca ataaaatctg tcagatacac agcatacaaa actgctatca
     1861 caatccactc ccaggttgca ggtgtatgta acgcatgtgc ctctacatac ttctggaaaa
     1921 cttttactac cataacgata gccagtcccc agatcagaga aaactccagg caaatgtagc
     1981 catgaaaatt aaagggttta tctgaataat cccaccatct tgcgtggaaa cacacatcca
     2041 gcagccagcc tgcaaccaac tccaccgcag ttgccagaac aattccaaac acaaaaatca
     2101 ttccatcact catctgacgc ccgctgccct gaattgtatt gagcaaagca aaaacagaaa
     2161 gcaccccaaa tccatatacc gggcagaccg gtccattcag gaaacctctg tttataactt
     2221 ttcccaacgc tactgcatga aaaataactt caacaaccca gccgccaaaa gaataaataa
     2281 gaaaatacag acatatctga aaatacgtca ttccacaaat cattcgttgc tcctccttgc
     2341 tttcatcaaa ccacttttca tatcccatta tcacagttca aacagaaaac ttaccccaga
     2401 ctgttttgta atgtactgat caattcctct ataacaattg gtttggatag aaatccatcc
     2461 ataccacatg ccagtgcttt ctttctgtct tcgtcaaaag cattggcagt catggcaagg
     2521 atcgtaattc ccgccagtgc cggatcatcc agtgaacgga tctgctcagt agcctcatat
     2581 ccgttcatta caggcatctg cacatccata agcaccagat cataatcacc tggtcttgaa
     2641 ttcttaacct tctccacagc ctctgctcca tcttccgctg tatcaacctg aaaaccatat
     2701 tcactaagga gtgctactgc aatttctctg ttcagttcat tatcttccac aagcagtatt
     2761 tgcctgcctc tgaaatctga atctgctgcc ggaagaatat tatcctctgc cccggcctgc
     2821 ttctgaccga tagcagccat cagagtctct ctgatatctg acataaacat aggtttggca
     2881 cagaacgcag tcacccctgc tgccctggct tccacttcaa tatctgacca gtcgtaagca
     2941 gtcaggataa tgattggcgt atcatcacca agactgcgga tctgacgggt aacttcaatg
     3001 ccattcatat caggcagacg ccagtcaatg atataagcgt ggaatgcatc ccccatttcc
     3061 atagactgtc gtgcacggag aacagcctcc ttaccggaaa gtgtccattc cgaacgcatt
     3121 ccaaccttta caagcatctt agtcacactg tcacaggtat tgaaatcatc gtctactacc
     3181 aatgccttca gaccttccag ttctgcgatt ttctctatac ggtggttctc aggctgaatc
     3241 cgcaatggca gacggataat aaattcagta cctttgccct gttctgtttg aacctcaatg
     3301 gttccgccca tcatatccac gatgttcttg gtgatagcca tgcccagtcc tgtaccct
//
LOCUS       DBMDXG010000243         3353 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6430_length_3353_cov_1.952337, whole genome shotgun sequence.
ACCESSION   DBMDXG010000243 DBMDXG010000000
VERSION     DBMDXG010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6430_length_3353_cov_1.952337"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(47..>306)
                     /locus_tag="ACZPD3_07155"
     CDS             complement(47..>306)
                     /locus_tag="ACZPD3_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415810.1"
                     /translation="AEAAGISASYLSRLFKKETGMLFVDYIQKERIEAACNMLTYSDY
                     TAAQISEYLCFSTQSYFIKIFRKYTGTTPAKYKKYKVQDWK"
     assembly_gap    307..316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<317..808)
                     /locus_tag="ACZPD3_07160"
     CDS             complement(<317..808)
                     /locus_tag="ACZPD3_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415811.1"
                     /translation="MQEKSKEYNEKEQQVAENRSRNTLFERRENLQKHESYASERRQY
                     DAIRNGQTDRIQSVFQITPDGTPGILSRNELRNSKNMFIAGITLFTRAAIEGGVPEET
                     AYALSDGYIQTVEECTSKSSIEKLSQKAALRFAQEVQKSGMRHYSREIEAAVKYIHLH
                     LHVP"
     gene            956..3241
                     /locus_tag="ACZPD3_07165"
     CDS             956..3241
                     /locus_tag="ACZPD3_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR1415812.1"
                     /translation="MKRDLKKIISQMTLEEKAGMCSGLDFWHLKSVERLGIPEVMVSD
                     GPHGLRKQDDKGDHLGMNDSIKAVCFPPAALSACSFDRSLMEAMGETIGREAQANDVS
                     VVLGPAVNIKRSPLCGRNFEYYSEDPYLAGEIAAAFINGVQSQHVGTSIKHFAANNQE
                     YHRMSNSSEADERTLREIYFPAFETAVKKAQPYTFMCSYNQINGTFASENKWLLTDVL
                     RNDWGFEGYVMSDWGAVNDRVKGLEAGLDLEMPGSNGTNDALIMEAVKNGTLKEEVLD
                     QAVERILNIIYKYADHRAPQEFTMEKDHEEARRIAEESMVLLKNADQILPLKTSEKVA
                     FVGGFAKKPRFQGGGSSHINCFKITNALEAVPADAQIIYAEGFPANKDLYDEKLAAEA
                     LQAAKAADKVVIFAGLPDSFESEGYDRSHMRLPECQNRLIGEILEVQPNTVIVLHNGS
                     PVEMPWINNVKGILEAYLGGQAGGAAVANILYGIVNPSGKLAETIPVKLADNPSYLNF
                     GGGDKVEYREGVFVGYRYYDTKQMEVAYPFGYGLSYTTFAYSNLQISNTNPTEKDTIT
                     VSVDVTNTGSIAGKEIVQLYVKDMTASTTRPEKELKGFEKVQLAPGETKTVTMELDKR
                     SFAWYNTELHDWYAASGKYEILIGASSRDIRLSETIELSSSQVIPMHIHMNTTLGELF
                     NNPDTKEAAKELTSRYLAAMNGGSDTASESAAEAITEEMVAAMTYSMPLRSLCSFGGF
                     SRAEIQEMIDKLQAIYSNPLK"
BASE COUNT          958 a          720 c          703 g          962 t
ORIGIN      
        1 actatagcaa ttaaaagata taatcaaaaa acttttgata ccgatattat ttccagtcct
       61 gaactttata ttttttatat tttgcaggtg ttgttcctgt atattttctg aaaattttta
      121 taaaatagct ctgtgtggaa aagcagagat attcactgat ctgtgctgct gtataatctg
      181 aataggtcag catattacag gcagcttcta tccgttcttt ctgaatatag tcgacaaaaa
      241 gcattcctgt ttctttttta aacaatctgg aaagataact ggcactgatc cctgctgcct
      301 ctgcagnnnn nnnnnnggga acatgcagat gaagatgaat atattttact gccgcttcaa
      361 tttctctgct gtaatgtctc attccggact tttgcacttc ctgggcaaat ctcagagctg
      421 ctttttgtga taacttttct atggaagatt tactggtaca ctcttccact gtctgaatat
      481 agccatcgct taatgcataa gcagtttctt caggtacgcc accttcaatc gcagctctgg
      541 taaacagcgt aattcctgcg ataaacatat tcttactgtt tcgcagttca tttctggaca
      601 gtattccagg agttccatcc ggtgtgatct gaaaaacgga ctgtattctg tctgtctgac
      661 cattcctgat agcatcatac tgccgacgct cacttgcata agattcatgt ttctgtaaat
      721 tctcacgtct ctcaaataga gtattcctgc ttcggttctc tgcaacctgt tgttcttttt
      781 cattgtattc cttacttttc tcctgcatct ttattcctct cgcatacgca gcaatatttt
      841 gttatttata tcaatatttt actatatttt atcatttgaa ggtgatatag tccagataaa
      901 ggcaacacaa acaatctgtt caacatatat taataaactt atggaggaat ttattatgaa
      961 gcgtgattta aagaaaatca tcagccagat gactctggaa gaaaaagcag gcatgtgctc
     1021 agggcttgat ttctggcatc tgaaaagcgt ggaaagactt ggaatcccgg aggtaatggt
     1081 aagcgacggt cctcacgggc ttcgtaagca ggatgacaaa ggagatcatc ttggaatgaa
     1141 cgacagtatc aaagcagtct gcttccctcc tgctgcttta agtgcctgct cttttgaccg
     1201 tagcttaatg gaagccatgg gcgaaactat tggcagagaa gcacaggcaa atgacgtatc
     1261 tgttgtttta ggaccggctg tcaatattaa acgttctccg ctctgcggaa gaaactttga
     1321 atactattca gaagatccat accttgcagg tgagatcgct gctgcattta tcaatggtgt
     1381 acagtcccag catgtaggaa cttccatcaa acattttgca gctaacaatc aggaatatca
     1441 ccgcatgagc aattcttctg aagcagatga aagaacactc cgtgaaattt attttcctgc
     1501 atttgaaact gcagtaaaaa aggctcagcc atataccttt atgtgctcct acaatcagat
     1561 taacggtact tttgcttccg agaataaatg gcttcttacc gatgttctcc gcaatgactg
     1621 gggatttgaa ggatatgtta tgtctgactg gggtgctgtc aatgaccgtg tgaagggtct
     1681 ggaggccggc ctggatctgg aaatgccagg ttccaacgga acaaacgatg ctctgatcat
     1741 ggaagccgta aaaaatggca ctctgaagga agaagttctg gatcaggctg tagaacgtat
     1801 cctgaatatt atttacaaat atgcggacca ccgtgcaccg caggaattta ccatggaaaa
     1861 agaccatgaa gaggcacgcc gtattgcgga agaatctatg gtccttctga aaaacgcaga
     1921 ccagattctg cctctgaaga cctctgagaa agtagctttt gtaggtggtt ttgccaaaaa
     1981 acctcgtttc cagggcggcg gaagctctca tattaactgc tttaaaatta ccaatgcact
     2041 ggaagctgtt cctgctgatg cacagattat ttacgcagaa ggatttcctg ctaataaaga
     2101 tttatacgat gaaaaactgg ctgctgaagc acttcaggct gcaaaagctg ctgataaggt
     2161 tgtgatcttc gccggacttc cggatagttt tgagtctgaa ggatatgacc gttctcatat
     2221 gagactgcca gaatgccaga accgtcttat tggtgaaatc cttgaagttc agccaaatac
     2281 tgtgatcgtt cttcacaatg gttctcctgt ggaaatgcca tggatcaata atgtgaaagg
     2341 tattctggaa gcttaccttg gcggacaggc aggcggagct gctgttgcaa atatcctcta
     2401 tggtattgta aatccaagcg gtaaactggc cgaaacaatt ccggtgaaac tggctgacaa
     2461 tccatcctac ctgaacttcg gtggcggcga taaagtggaa taccgtgaag gtgtatttgt
     2521 aggatatcgt tattacgata ccaagcagat ggaagttgct tatccattcg gatacggact
     2581 ctcctatact acttttgcat acagcaatct gcagatttcc aacacaaatc caacagaaaa
     2641 agataccatc actgtttctg tagatgtgac aaatacaggc agcattgcag gaaaagaaat
     2701 tgtccagctc tatgtgaaag acatgacagc atccacgacc cgtcctgaaa aagaactgaa
     2761 aggctttgaa aaagtccagc ttgctcctgg cgaaaccaaa acagttacta tggaactgga
     2821 taagcgcagc tttgcatggt acaataccga attacacgac tggtatgctg caagcggcaa
     2881 atatgagatt ctgatcggtg catcctccag agatatccgt ctctctgaaa ccattgaact
     2941 ctccagcagc caggtaattc ctatgcatat tcatatgaac acaacactgg gtgaactttt
     3001 caataatcca gacacaaaag aagcagcaaa agaactgact tccagatatc tcgctgcaat
     3061 gaacggcggt tcagacacag cttccgaatc tgctgctgaa gctatcactg aagaaatggt
     3121 agcagcaatg acatattcca tgccacttcg ctctctgtgc agctttggcg gattctcccg
     3181 ggctgagatc caggagatga ttgacaaact gcaggcaatc tatagcaatc ctctgaaata
     3241 atgcatctta atccagccca tcagagtgtg aaatcctgcg tcagatatct ttgccgtgcg
     3301 tcagcacgcc tgcagatatc ttccttgtct ttcgcactct gctgaactgt ctt
//
LOCUS       DBMDXG010000244         3340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6452_length_3340_cov_1.377894, whole genome shotgun sequence.
ACCESSION   DBMDXG010000244 DBMDXG010000000
VERSION     DBMDXG010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6452_length_3340_cov_1.377894"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="ACZPD3_07170"
     CDS             complement(<1..572)
                     /locus_tag="ACZPD3_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR1415813.1"
                     /translation="MGGESKNGKVPLISKIAYGFGDVGCNFSWMFVSNFLMIFYTDVF
                     GISMAAVSALMLFSRFWDAINDPIVGGLTDKTKSRWGRYRPWLLVAAPITAILLVMTF
                     WARPDWPQNGKIIYMVITYCLLVLGYTCVNIPYGTLCGAMTQDIDERAKINTSRSVAA
                     MIAIGVINIITVPLLSKFGSHSAKTALGDV"
     gene            complement(591..983)
                     /locus_tag="ACZPD3_07175"
     CDS             complement(591..983)
                     /locus_tag="ACZPD3_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR1415814.1"
                     /translation="MENKYGIVGIAHVGLPTNDLQKTVEFYKSLGFEVIMQSYNEKAG
                     EKVAFLQIKNYCIETFENGKAEMSDGAYQHVALDVEDIESMYQKICNEKYTVITDGIE
                     ELPFWENGVKFFMIKGPNEERIEFCQKI"
     gene            complement(993..2021)
                     /locus_tag="ACZPD3_07180"
     CDS             complement(993..2021)
                     /locus_tag="ACZPD3_07180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR1415815.1"
                     /translation="MLQQVMTNPGEIIFREVPVPEVKENQVLVKIMNIGVCGSDIHVY
                     HGKHPFTKYPVTQGHEVSGEITELGKNVTEFHVGQKVTIEPQVYCGHCYPCRHGKYNL
                     CEELKVMGFQTTGTASEYFAVDASKVTPIPEDMSYEEGAMIEPLAVAVHGVKQMGDVA
                     GMNIAVLGAGPIGNLVAQTAKGMGAAKVMITDISDLRLAKAKECGIDVCVNTKNKDFG
                     EAMIEAFGPDKADVIYDCAGNNITMGQAIKYARKGSTIVLVAVFAGMAEVDLAVANDH
                     ELDIKSTMMYRHDDYIDGIRLVNEGKVHLKPLISKTFAFKDYLKAYQYIDDNRETTMK
                     VIINVSEK"
     gene            complement(2346..>3340)
                     /locus_tag="ACZPD3_07185"
     CDS             complement(2346..>3340)
                     /locus_tag="ACZPD3_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR1415816.1"
                     /translation="LSNVKDLLEKGIIIENGEYESTGGRKAKSIGINPSYRYAMGIVI
                     TANHLGMVLVNLKYEIVKSERIRLKFVSDMSYCSQVADYAAKFLDHMNDAEQKEKLLG
                     VGISIPGIINQEQKLVIKSHALKLENYSLSFLEQAFSVPVYFSNDANAAMMAEDMNIY
                     QNAIYLSLNQTLGGAFCIGGNLFSGENQKAGEFGHMILVPQGRKCYCGKSGCADAYCA
                     AGALVGESKDSVEQFMQLLQNNDEKAEKKWEEYLDYLAILISNLRMAYDMDIILGGEM
                     GGYLSDYMIPLGEKVMKYNGFDHDLRYLKNCSYKKEASAVGAAKHFLQDFIGKI"
BASE COUNT          927 a          810 c          486 g         1117 t
ORIGIN      
        1 tgaacatcac ccagcgcggt ctttgcacta tgactgccga atttgctgag cagtggaact
       61 gtgataatat ttatcacacc aattgcaatc atcgctgcaa ccgaacggga tgtatttatt
      121 tttgcacgtt catcaatatc ctgtgtcata gcaccacaca aggttccata tggaatattc
      181 acacaggtat accccaaaac cagcagacaa tatgtaatta ccatataaat gatctttcca
      241 ttctgcggcc agtcaggtct tgcccagaat gtcatgacaa gaagaattgc tgtaattggt
      301 gccgctacta acagccatgg acggtaccgt ccccatctcg attttgtttt atccgtaagt
      361 cccccgacga ttggatcatt gattgcatcc cagaatcttg aaaaaagcat cagtgctgac
      421 acagccgcca tactgattcc gaacacgtct gtataaaata tcatcagaaa attactgaca
      481 aacatccagc tgaaattaca tccaacatca ccaaacccat atgcaatctt actgatcagc
      541 ggtacttttc catttttgct ttcgcctccc atatgtccct cctattttta tcagattttc
      601 tgacagaact ctattctttc ctcatttggt cccttaatca taaaaaactt cacaccattt
      661 tcccaaaatg gcagttcttc gattccgtct gtaataactg tgtatttttc attgcagatt
      721 ttctgataca tactttcaat gtcttccaca tcaagtgcaa catgttggta tgcaccgtct
      781 gacatttccg ccttcccatt ttcaaaagtc tctatgcagt aattttttat ctgtaaaaat
      841 gccacttttt cccctgcttt ttcattataa gactgcataa ttacttcaaa tccaagactc
      901 ttataaaact caactgtttt ctgtaaatcg tttgtcggaa gcccgacatg ggcaattccg
      961 acaattccat atttattttc catacgaact ccttactttt cactgacgtt gataattact
     1021 ttcatcgttg tctcgcggtt atcatcaata tactggtatg ccttcagata atctttaaaa
     1081 gcaaatgtct ttgagatgag cggtttcaaa tgaactttgc cctcatttac cagtctgatt
     1141 ccatctatgt aatcatcatg gcggtacatc atcgtgctct taatgtcaag ttcgtggtca
     1201 tttgccactg caagatctac ttctgccatt cctgcaaaaa cagccaccag tacgatcgtg
     1261 cttccttttc ttgcatattt aattgcctgt cccattgtga tattgtttcc ggcacagtcg
     1321 tagatcacat ctgccttatc cggtccaaat gcttcgatca tcgcctcacc gaagtcttta
     1381 tttttggtat tcacacacac atcaattcca cattcttttg ccttcgccag tctaagatca
     1441 ctgatatctg tgatcatcac tttcgctgct cccattcctt ttgctgtctg tgccacgaga
     1501 tttccgatcg gtcctgctcc aaggactgca atattcatgc cagccacatc gcccatctgt
     1561 tttacaccat gaactgccac agcaagaggt tcgatcatag ctccttcttc ataggacata
     1621 tcttctggaa taggtgttac tttagatgca tccactgcaa aatactcaga tgctgttcct
     1681 gtcgtctgaa atcccattac ttttaattcc tcacagagat tatactttcc atgacggcat
     1741 ggatagcaat gtccacaata tacctgcggc tctatcgtca ctttctgtcc aacatgaaac
     1801 tctgtgacat tttttccaag ttctgtaatc tcacctgaaa cctcatgtcc ctgtgttacc
     1861 ggatattttg taaacggatg tttcccatga taaacatgaa tatctgatcc acagacacca
     1921 atattcataa tctttaccaa cacctgattt tcctttacct ccggaaccgg aacctctctg
     1981 aaaataattt ctcctggatt tgtcattacc tgctgtaaca taattgtttc ctcctttttt
     2041 atattgtttt attttatgtt ttataaaatg ttttattaaa gtaattacat tattattcaa
     2101 atcgctaaat ttgtcaataa acatttaaaa atatttctca tttctaccag ttgcacaaag
     2161 tatagatttt atttttatgc aaatgttact ataacactcc tcataattat ctgctgaatt
     2221 cccgcacaat aacaaaatac cctgattaca acctttcttt gttgtaatca ggatattttt
     2281 tatattcaga tatttgttta atatggtata tcagcagatc atcagtgatt caagaatgcc
     2341 atcggctata tctttccgat aaaatcctgc agaaaatgct ttgctgcccc tacagctgag
     2401 gcttctttct tataagaaca gtttttcaga tatctcagat catgatcaaa tccattatac
     2461 ttcattactt tttccccaag aggtatcata taatctgaga gatatccacc catttcacct
     2521 ccaagaatga tatccatatc atatgccatg cgcagatttg aaatcagaat tgcaagataa
     2581 tcaagatact cttcccattt tttctctgcc ttttcatcat tgttttgaag cagctgcata
     2641 aactgttcca cagaatcctt tgactcacca acaagtgcgc ctgcagcgca ataagcatct
     2701 gcacaacctg attttccaca ataacacttg cggccctgag gtacaagtat catatgtcca
     2761 aattctccgg ccttctgatt ttctccggaa aacaaatttc cccctatgca aaatgcaccg
     2821 ccaagagtct gattcaacga aaggtaaatt gcattctgat agatgttcat atcttctgcc
     2881 atcattgccg cattggcatc atttgaaaaa taaaccggga cagaaaatgc ctgttccagg
     2941 aaactgagac tatagttttc aagtttcagt gcgtgggatt ttatcaccag tttctgctcc
     3001 tgattaatga ttcccggaat agagattcca acacccagaa gcttttcttt ctgttctgca
     3061 tcattcatat gatccagaaa tttcgccgca taatctgcca cctgactgca atatgacata
     3121 tcagatacaa atttcagacg gatccgctct gattttacaa tctcatactt cagatttacc
     3181 agcaccattc ccagatgatt tgcagtaatc acaattccca ttgcataacg ataggatgga
     3241 ttgattccaa tacttttagc ctttcttcct cctgtggatt catattctcc attttctatt
     3301 atgatacctt tttccaataa atcttttaca ttagacagaa
//
LOCUS       DBMDXG010000245         3330 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6472_length_3330_cov_2.125426, whole genome shotgun sequence.
ACCESSION   DBMDXG010000245 DBMDXG010000000
VERSION     DBMDXG010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6472_length_3330_cov_2.125426"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="ACZPD3_07190"
     CDS             complement(<1..268)
                     /locus_tag="ACZPD3_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1415817.1"
                     /translation="MRTKREDVRNVAIIAHVDHGKTTLVDQLLKQSGVFRENQEVQER
                     VMDSNDIERERGITILSKNTAIHYKDTKINIIDTPGHADFGGEVE"
     gene            694..1320
                     /locus_tag="ACZPD3_07195"
     CDS             694..1320
                     /locus_tag="ACZPD3_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR1415818.1"
                     /translation="MADKIIENNQVSMMGKIAAGFTFSHQVFGEGFYMTELLVKRLSD
                     SEDRIPVMVSERLVDVTQDYIGEYVEIHGQFRSYNRHEEKHNRLVLSVFSRELKFVEE
                     EDETVPVNQIFLDGYICKPPVYRKTPLGREIADVLLAVNRPYGKSDYIPCICWGRNAR
                     YASAFEVGGHVLIWGRIQSREYMKRIGENETEKRVAYEVSVSKLEYME"
     gene            1602..2093
                     /locus_tag="ACZPD3_07200"
     CDS             1602..2093
                     /locus_tag="ACZPD3_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR1415819.1"
                     /translation="MEKEFTEVYCGGGKGKTTLAIGQCLRASAQGKSVIIIQFLKGRE
                     RRELDFLEELDNLDIKIFRFEKHDEGYEELNEQEKAEEKTNILNALNFARKVIATQEC
                     DFLLLDEILALPDYGITTAEAIGDILKMKDESMHILLTGRTLPDSLRKYADSITTLTT
                     EIV"
     gene            2173..3066
                     /gene="dapA"
                     /locus_tag="ACZPD3_07205"
     CDS             2173..3066
                     /gene="dapA"
                     /locus_tag="ACZPD3_07205"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR1415820.1"
                     /translation="MAIFKGAGVAIVTPMYEDGKVNYDKLEELLEYQIANSTDAIIIC
                     GTTGESSTMTHGEHLKTIKFAIDKVNKRVPVIAGTGSNCTETAIMMSKEAASYGADAL
                     LIVTPYYNKATQKGLIAHYTAIAEAVPETPIIMYNVPSRTGCNIQPATAVALAKNVKN
                     IVGIKAASGDLSQIATMMSMADGCIELYSGNDDQVLPILSLGGLGVISVLSNVAPKET
                     HDMVMKFLEGDTKGAAEIQLKAIPLIHALFSEVNPIPVKAALNLMGKEVGPLRMPLSE
                     MEDANKAKLAEELKNFGIKLA"
     gene            3132..>3330
                     /locus_tag="ACZPD3_07210"
     CDS             3132..>3330
                     /locus_tag="ACZPD3_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1415821.1"
                     /translation="MVKIIMHGCNGHMGQVISGLVEKDPDAEIVAGIDVADQGKNSYP
                     VYTNMEECQVEADALIDFSSAK"
BASE COUNT         1024 a          598 c          811 g          897 t
ORIGIN      
        1 gttctacctc gccaccgaaa tcagcatgtc ctggtgtgtc gatgatatta atctttgtat
       61 ctttgtaatg aatagctgta ttcttggata aaatagtgat accacgctca cgctcaatat
      121 cattggagtc catgacacgt tcctgaactt cctgattttc gcggaataca ccgctctgtt
      181 tcagaagctg gtcaaccaga gttgttttac catggtcaac gtgggcgata atggctacgt
      241 tacggacatc ttcacgtttt gttctcataa aatgattttc ctttcctctt taagctttga
      301 atggataact gttcggcaca ctggctgaac agttaccgga attgttaata ttcatgtgtt
      361 atcagcagga ttattttgct gcaaaagcat ttttcaagca cgatataatc ttgcagatga
      421 atttgttcct attattatgt gtttttttct tcaataagtt acccgaattt tcagtttacc
      481 acattttcgc cagattacaa ggtgttaaaa taattttttt tatttttgca gattctggtt
      541 ctaaatgaga atgcctgtac ataaccttat aaagaacaat acaaggtaca gaggagcata
      601 tgcgggatcg ttgaaaaagc agagacgatt tacggtctgc actgcaggca gggattctga
      661 cacaggtgct ctacagggaa ggagaaccac attatggcag ataaaattat tgaaaacaat
      721 caggtatcta tgatgggcaa aattgcagca ggctttacat tcagtcatca ggtctttgga
      781 gaaggcttct atatgactga gctgctggtc aaaaggctca gtgattcgga agacaggatt
      841 ccggtgatgg tttctgaacg gctggtggat gtaacgcagg attatatcgg agaatatgtg
      901 gaaatccatg gacagttccg ctcatataac cgccatgagg agaagcacaa ccgtctcgtg
      961 ctttcagtat tcagcagaga actgaagttt gtggaagaag aggatgaaac agttccggta
     1021 aatcagattt ttctggacgg atatatctgt aagcctccgg tgtaccgtaa aactcctctt
     1081 ggaagagaaa ttgcggatgt gctgctggca gtaaatcgtc cgtatggaaa atcagattac
     1141 ataccatgca tctgctgggg cagaaatgcc cgctatgcat ctgcgtttga agttggcggt
     1201 catgtcctga tctggggacg tatccagagc agagaatata tgaaacggat cggggaaaat
     1261 gaaacagaga agagagttgc gtatgaagta tctgtaagca agctggaata tatggaataa
     1321 ttccattgcc gcaggcaatt tggaatgaag tttggccacg ttactgtgaa cggagtgaac
     1381 ggtaacgatt actgtaaaca gtaacacttg caattcttca taaactgcgg taatataagc
     1441 gtattcagat aactatggca gcctgctgtt ccacgaagca ttccggcgtg aatatgccgg
     1501 aagctcatcg cagaagtcga aaacagactg ctgtttatca tgtctgtaat gacacagaga
     1561 agaaggtaca atttcaataa cgataagagg aattgtttgt tatggaaaaa gaatttacag
     1621 aggtttactg tggcggggga aagggaaaga caactctggc gatcggtcag tgtctgagag
     1681 cttccgcaca gggtaaaagc gtcattatta ttcagtttct gaaaggaaga gagcgcaggg
     1741 aactggattt tctggaggaa ctggataatc tggatattaa gatattccgt tttgagaagc
     1801 atgatgaagg atatgaggaa ctgaatgagc aggagaaggc agaggagaaa accaatattc
     1861 tcaatgcttt gaattttgcg cgtaaagtga tcgcaacgca ggaatgcgat tttctgctcc
     1921 tggatgagat tcttgcactt ccggattatg ggatcactac tgcagaggca atcggtgata
     1981 tcctgaagat gaaggacgag agtatgcata ttttgcttac gggacggacg ttaccggaca
     2041 gcctgcgtaa atatgcagac agcataacta cactgacaac agagattgta taaggtgtat
     2101 aaaacctgat tattgtttat gaatgggcag taatctgtat ggaaattaca gaagaataaa
     2161 ggaggaaggc aaatggcaat ttttaaaggt gcaggtgttg caatcgtaac ccctatgtat
     2221 gaagatggca aggttaatta tgataaactg gaggaacttc tggagtatca gattgcaaac
     2281 agtacagatg cgatcattat ctgcggaaca acaggagaat catcaacgat gacgcatgga
     2341 gagcatctta agacaattaa gtttgcaatc gacaaggtaa acaaacgtgt tccggttatc
     2401 gcaggtacag gttccaactg tacggaaact gcaattatga tgtcaaagga ggctgcttct
     2461 tacggtgcag atgcacttct tatagtgact ccatattata acaaggcgac tcagaaagga
     2521 ctgatcgctc attatacagc tattgcagag gcagttccgg aaacaccgat cattatgtac
     2581 aatgttccga gccgtacagg atgtaacatc cagcctgcaa cagcagtagc tctggctaaa
     2641 aatgttaaga atattgttgg tatcaaagca gcaagcggag atctttcaca gattgcaacc
     2701 atgatgtcca tggctgatgg ctgcattgaa ctctactccg gaaatgatga tcaggtactt
     2761 ccgatccttt cacttggcgg acttggagta atttccgtac tttctaatgt agctcctaag
     2821 gaaacacatg atatggttat gaaattcctt gaaggagata caaagggtgc agcagaaatc
     2881 cagttaaagg caatcccgtt aattcatgca ctgttcagtg aagtaaaccc gattccggtg
     2941 aaagcagctc tgaatctgat gggtaaagaa gtgggaccac ttcgtatgcc attatccgag
     3001 atggaagatg caaacaaggc aaaacttgct gaagaactga agaatttcgg aattaaactt
     3061 gcataaatat aaccgcagga ttgttcggaa aatcaacttg ccgatatact ggaattgaga
     3121 ggaatagaca aatggtaaag attattatgc atggctgtaa cggtcacatg ggtcaggtaa
     3181 tctccggact tgtggagaag gatccggatg ctgagatcgt agcaggtatc gatgtggcag
     3241 atcagggaaa gaacagctac cctgtatata caaatatgga agaatgtcag gtagaggcag
     3301 atgcccttat cgacttctct tctgcaaaag
//
LOCUS       DBMDXG010000246         3328 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6476_length_3328_cov_2.025101, whole genome shotgun sequence.
ACCESSION   DBMDXG010000246 DBMDXG010000000
VERSION     DBMDXG010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6476_length_3328_cov_2.025101"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(183..1043)
                     /locus_tag="ACZPD3_07215"
     CDS             complement(183..1043)
                     /locus_tag="ACZPD3_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013899.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HIR1415822.1"
                     /translation="MDNKFGKISRISACAALKDGRTILEDLSFTAPYKIMMPFEKENG
                     GIQIMPLCASAGIMAGDSQEFSYHVKEGADLEVLSQSFEKIHKMDEGSAARTIEVQVD
                     KNATLYYYPQPVIPFAQSAFDSKMTIHLEDETSRLFLLEIISCGRNAHDERFQYRRFS
                     SKVLLYRGDKLIYRDNTSYEPDKMPMEGIGMYEGYTHMANLFLSKICSRDGESCSQES
                     GSVKSADSAINLELQEKIWQILDEDSEIDGGVTRLTTGDLALRIFGYRAQKLQQVAEK
                     IKKLYEKEQI"
     gene            complement(1056..1664)
                     /gene="ureG"
                     /locus_tag="ACZPD3_07220"
     CDS             complement(1056..1664)
                     /gene="ureG"
                     /locus_tag="ACZPD3_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HIR1415823.1"
                     /translation="MSYVKIGVAGPVGSGKTALIEALSRKMAKDYSIGVITNDIYTKE
                     DAQFLAKNSVLPVERIIGVETGGCPHTAIREDASMNLEAVDEMMERFPDIELLFIESG
                     GDNLSATFSPELVDATIFVIDVAEGDKIPRKGGPGITRSDLLVINKIDLAPYVGASLE
                     VMERDSKKMRGDRPFQFTNIRGDENVDKVVEWIKKNVLLEGM"
     gene            complement(1817..2509)
                     /locus_tag="ACZPD3_07225"
     CDS             complement(1817..2509)
                     /locus_tag="ACZPD3_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HIR1415824.1"
                     /translation="MSEKQFYLLQVNDALFPIGGYSHSQGLETYIQRGIVHNVDTARE
                     YITHKIKWNLAYTELLAARLAYEAAEKKDLQELLYLEELLEASRIPMEQREAARKMGS
                     RFAKTIEKLGLSISETGIFREYLDARKGKAVNHCCIYGVFCAEMQIPLEEALTHYLYA
                     QTSAIVTNCVKTIPLSQTSGQQLLSGCYGEFDEILKDVMNRSEEDLCLSAPGFDIRGI
                     QHEKLYSRLYMS"
     gene            complement(2528..3004)
                     /locus_tag="ACZPD3_07230"
     CDS             complement(2528..3004)
                     /locus_tag="ACZPD3_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HIR1415825.1"
                     /translation="MLCEQVLGKLHDFDITGKTIEYVDIEWHEAFKKIHKKITDKGTE
                     VGIRMDDSILARGLYQDDVIYADNEKLVVVNTPPCEVIRVSLTPGHEKMSANVCYEIG
                     NRHAPLFWGENDTFITIYNEPMLVMLQKIHGVQAEKEVLKLDFDKRISASIHNHHH"
     gene            complement(3085..>3328)
                     /locus_tag="ACZPD3_07235"
     CDS             complement(3085..>3328)
                     /locus_tag="ACZPD3_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AmiS/UreI family transporter"
                     /protein_id="HIR1415826.1"
                     /translation="VAINAVIFGTIEGFTGSTALGITPDLRWAAIWYLWAILWGTAFV
                     EDICGKKLGKFVPYLQVFEGIVTAWVPGVMMLLQIW"
BASE COUNT          812 a          785 c          659 g         1072 t
ORIGIN      
        1 gacaattccg gtatttcttc tgctggggtg gctgattcct tgcatgatgt atgacaacgc
       61 agcgaaatgc agcgttgtag agcaattaag ggatgctcaa tgattctccg cctgcggcgg
      121 gtcgcctcag cgacataatt ctatacatca gcacaaaaaa tggcgggatt tcccgccatt
      181 ttttatattt gttctttttc gtataatttc ttgatttttt ccgctacctg ctgcagtttc
      241 tgagcacgat aaccgaagat tctcaacgcc agatctccgg ttgtgagacg ggtaacgcct
      301 ccatctattt ccgaatcttc atcaaggatc tgccagattt tttcctgaag ttccagattg
      361 attgctgaat ctgctgactt tacactcccg gattcctgac tgcaactttc accatctctg
      421 ctgcagatct tagaaagaaa caggtttgcc atatgtgtgt agccctcgta cattccgatt
      481 ccttccatgg gcattttgtc cggttcgtag ctggtattat cacgataaat cagtttgtct
      541 cctctgtaca acaggacttt tgaggagaat ctgcggtact gaaaacgttc gtcatgagcg
      601 tttcgaccac aggagatgat ctccagcagg aataaacggg aggtttcgtc ttccagatgg
      661 atagtcatct tgctgtcaaa ggctgactgt gcgaaaggaa tcaccggctg tggatagtaa
      721 tataatgtgg cgtttttgtc tacctgcact tcaatggttc gggcggctga gccttcgtcc
      781 attttgtgga ttttctcaaa ggactgggac aggacttcca ggtctgcgcc ttcctttaca
      841 tgataggaaa attcctggga atcgcctgcc ataattcctg cggaagcaca gagcggcatg
      901 atctggatcc caccgttttc tttttcgaaa ggcatcatga tcttgtatgg tgctgtaaaa
      961 gacagatctt ccagaatagt ccttccgtct tttaaggctg cgcaggcgct gatgcgggat
     1021 atttttccga atttgttatc cataggcatt ttcctttaca ttccttccag gagtacgttt
     1081 ttcttgatcc actctaccac tttatctacg ttctcatctc cgcggatatt tgtaaactgg
     1141 aatggtctgt caccacgcat ttttttggaa tctctttcca ttacttcaag acttgcacct
     1201 acatatggag ccaagtctat cttattaatt acaagaagat cagaacgtgt gattccagga
     1261 ccgcctttac gcggaatctt atctccttct gctacatcaa tgacgaagat ggttgcatct
     1321 accagctccg gactgaatgt tgcagacagg ttatctccgc cgctttcgat aaaaagaagt
     1381 tcaatatcag ggaagcgttc catcatctca tctactgctt ccagattcat ggaagcgtct
     1441 tcacgtatgg ctgtgtgagg gcatccgcct gtctctacac caatgatcct ctctacagga
     1501 agaacgctgt tctttgcaag gaactgggcg tcttcttttg tgtagatatc atttgtgatg
     1561 acaccaatgc tgtaatcctt tgccatcttt cttgacagtg cttcaataag tgctgttttt
     1621 ccggaaccca caggtccggc aactccgatt tttacgtatg acataaccga tttcctcttt
     1681 tctactttac ttgtttatag cttcttataa tctgtttact gactttccac atattatttt
     1741 tgaagatttc atattataca catgattctg tttctatcca cgcttgagcc tggaataacc
     1801 taataccaaa ttcaagctac gacatatata gcctggaata caacttctca tgctggattc
     1861 ctctgatatc aaatccaggt gcagagagac ataagtcctc ctcgcttctg ttcatgacat
     1921 cctttaatat ctcatcaaat tccccatagc atccgctcag aagctgctgc ccggatgtct
     1981 ggctgagggg gattgttttt acgcagttgg tgacgattgc agatgtctgg gcatatagat
     2041 aatgtgtcag cgcctcttcc agaggaatct gcatttctgc acagaaaact ccgtaaatac
     2101 agcaatgatt tactgctttt ccctttctcg catccagata ttccctgaaa atccctgttt
     2161 cactgatgga aagtccaagc ttttcaatgg tcttggcaaa acgggaaccc atttttctgg
     2221 cagcttctct ctgttccata ggaattctgg atgcttccag aagttcttcc aggtacagaa
     2281 gttcctgcag gtcttttttc tctgcagctt cgtatgcaag tctggctgcc agaagttctg
     2341 tatacgcaag attccatttg attttgtgag tgatgtactc tctggctgtg tctacattat
     2401 ggacaattcc acgctgaata taagtctcaa gaccctggga gtgggaatat cctccgatgg
     2461 gaaacagcgc atcatttacc tgtagaagat agaactgttt ttctgacatg aagaacctcc
     2521 gttgttatta atgatgatga ttatgaatac ttgcagaaat tctcttgtca aagtccagct
     2581 tcaacacttc tttctctgcc tgaactccat ggattttctg aagcataaca agcattggct
     2641 cattatagat cgtgataaac gtatcattct ctccccagaa aagcggtgcg tgtctgtttc
     2701 cgatttcata gcagacattt gcagacattt tctcatgtcc gggagtcaga gaaacacgga
     2761 taacttcgca tggcggtgtg tttaccacta caagcttctc attatctgca tagatcacat
     2821 catcctgata cagacctctg gcgagaatgg aatcgtccat gcggattccc acttctgttc
     2881 ctttgtctgt tattttttta tgtattttct taaatgcttc gtgccattcg atatctacat
     2941 attctattgt ttttcctgta atatcaaaat catgaagctt tcctaaaacc tgttcacaca
     3001 gcattatttt caacctccag aatctgcttt atgttttatt aataccacct gttctccaat
     3061 tgataaggtt tattgattgt gttcttacca aatctgaagt agcatcataa ctccaggaac
     3121 ccatgcagtt acgattcctt caaatacctg aagatatgga acgaattttc ccagtttctt
     3181 tccgcagatg tcttctacga aggcagttcc ccagagaatt gcccacaaat accagattgc
     3241 tgcccaacgc agatccggtg tgattcccag tgcagtgctt cctgtaaatc cctcgattgt
     3301 accgaagatc accgcattga tggctaca
//
LOCUS       DBMDXG010000247         3323 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6489_length_3323_cov_1.565177, whole genome shotgun sequence.
ACCESSION   DBMDXG010000247 DBMDXG010000000
VERSION     DBMDXG010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6489_length_3323_cov_1.565177"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(290..2245)
                     /gene="ftsH"
                     /locus_tag="ACZPD3_07240"
     CDS             complement(290..2245)
                     /gene="ftsH"
                     /locus_tag="ACZPD3_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR1415827.1"
                     /translation="MNLDNKKPGRKPLYVYYIIAAVIIILLNVLLLPAIEGRKVQTTD
                     YSTFLTGVEKDYVKTVEIQDDYIYYVAESDGQEGYFRTVKMNDPDLVDRLHDANVKFG
                     AAAPQQTSIWISLIAGYVLPIAIFILLGRWLSKKMMSSMGGGPGGAMSFGKSNAKVYV
                     KSSTGIKFSDVAGEDEAKDLLTEIVDYLHNPQKYREIGASMPKGALLVGPPGTGKTLL
                     AKAVAGEAEVPFFSISGSEFVEMFVGMGAAKVRDLFKQANEKAPCIVFIDEIDTIGKK
                     RDGAGFTGGNDEREQTLNQLLTEMDGFDGSKGVVILAATNRPDSLDPALLRPGRFDRR
                     IPVELPDLKGREEILKVHAKKIKIADSVRFDEIAKAAAGASGAELANIVNEAALRAVR
                     DGRKFATQADFEESIEVVIAGYQKKNRVLSNKEKLIVAYHEIGHALVAAKQTESAPVH
                     KITIIPRTSGALGYTMQVDDGDHYLMTKEELANKIATFTGGRAAEELIFHSITTGASN
                     DIEQATKLARAMISRYGMSEDFDMVAMENVTNQYLGGDSSLSCSFETQTLLDKKVVEL
                     VRMEHQKALKILQDNIGKLHELAKYLYEHETITGEEFMKILNAPVQVPTAVAESESNT
                     KSENNAESENSTEADADTSSGKVNLQK"
     gene            complement(2418..2500)
                     /locus_tag="ACZPD3_07245"
     tRNA            complement(2418..2500)
                     /locus_tag="ACZPD3_07245"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2465..2467),aa:Leu,seq:caa)
     gene            complement(2564..>3323)
                     /locus_tag="ACZPD3_07250"
     CDS             complement(2564..>3323)
                     /locus_tag="ACZPD3_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR1415828.1"
                     /translation="LSLKPQESDIHRKWEIGSAKGIYTKVERAYNIYVFFQREEYTGG
                     IWEKNDIVQPVRYQFNAAPLTEQGGSYKYLIGGIGYKILNEREVSVTGLAAEYNVIQI
                     PATVVINDKVYKVTTIDKNAFSGNKEITDVIFGNNVTTIGKYAFSQCPNLRNIRFGSR
                     VKRIGSNAFAQCTKLRNFILPASVRHIDARAFYQCPAVKVIRINSTALNYVGKKAFAV
                     NKTVTIRLPEKLFARYQKLIKASNVYSKTRFVKY"
BASE COUNT          851 a          801 c          615 g         1056 t
ORIGIN      
        1 ctcttccagt tcatttaacg gaagatcatg tttaatatac ctgctcacca ttccttctgc
       61 agtttggcaa tccatcttca ttcccttctt tcttccaaaa aaacatccag gatgctgcgt
      121 tcactattac gaattataac cgaaccggag ttaattggca agaaaaagca gcagaagctc
      181 aaatcgcctc tgctgctttc attgctgttc actcagtttt cagtgatata gccaattttc
      241 cggattgcct gcgacaatgg acttttgact tgtatatctc gaaaaaatat cacttctgca
      301 gatttacttt cccggaagat gtatctgcgt cagcttctgt actgttttcg gattctgcat
      361 tgttctcaga ctttgtatta ctttcagatt ccgccactgc agtgggaacc tgtacaggtg
      421 cattcaggat tttcataaat tcctcacctg taattgtctc atgttcatac agatattttg
      481 caagttcatg gagctttcca atattgtcct gaaggatttt cagtgctttc tggtgttcca
      541 tcctgacaag ttccacaact ttcttatcca gaagagtctg tgtctcaaaa gaacagctta
      601 agctggagtc tccgcccaga tactgatttg tcacattctc catggcaacc atatcgaagt
      661 cttcactcat accatatctg gagatcattg ctcttgcaag cttagtcgcc tgttcgatat
      721 cattggatgc tccggttgta atagagtgga agatcagttc ctcagcggca cgaccgcctg
      781 taaaggtcgc tatcttattt gcaagctctt cctttgtcat cagataatga tctccgtcat
      841 ccacctgcat ggtatatccg agtgctcctg atgtacgcgg aataatagtg atcttgtgga
      901 ccggtgcaga ttcggtctgc tttgctgcaa ccagcgcatg tccgatttca tgataagcaa
      961 ctatcagttt ttctttattg gaaagaactc tgttcttctt ctgatatcct gcgataacta
     1021 cctcgatact ctcctcaaaa tctgcctggg tggcaaattt tctgccgtca cgtactgcac
     1081 gaagtgctgc ttcattgaca atatttgcaa gttctgcacc ggaagctccg gctgcagctt
     1141 tcgcaatctc atcaaaacgt acggaatctg cgatcttaat cttctttgca tggactttaa
     1201 ggatttcctc acgtcctttt aaatccggaa gttcaacagg aatacgtctg tcaaaacgtc
     1261 cgggacgaag aagtgcggga tcaagggaat ccggtctgtt ggttgccgca aggatcacaa
     1321 cacctttgga gccgtcaaaa ccatccatct ctgtaagcag ctggttcaga gtctgctcgc
     1381 gctcatcatt gccgcctgta aatccggcac catcacgttt cttgccaatt gtgtcgatct
     1441 catcgataaa tacaatacat ggagctttct cattcgcctg tttgaaaaga tcacgcacct
     1501 tggcagcgcc cataccaaca aacatttcca cgaattcaga accggaaatg gagaagaacg
     1561 gaacttccgc ttcaccggct accgcttttg caagaagtgt tttacctgta cccggagggc
     1621 ctacaagaag ggctcctttc ggcatagatg caccgatttc tctgtatttc tgcgggttat
     1681 gaagataatc tacgatctct gtcagaagat ccttggcttc gtcctctccc gctacatctg
     1741 agaatttgat tcccgtagat gatttcacat atactttcgc attactcttt ccaaatgaca
     1801 ttgccccacc gggacctccg cccatggaac tcatcatctt cttgctgagc caccttccaa
     1861 gaaggataaa gatggcaatc ggcagtacat atcctgcgat caggctgatc cagatagatg
     1921 tctgctgtgg tgctgctgca ccgaacttga catttgcatc atgcaaacgg tctacaaggt
     1981 caggatcatt catcttgaca gttctgaaat aaccctcctg tccgtcactc tctgctacat
     2041 aataaatata atcatcctga atttctactg tcttaacgta atccttctct actcctgtca
     2101 ggaaagtact gtaatcagtc gtctgtacct ttcgtccctc aattgcagga agtaacagca
     2161 cattcagaag aatgataata actgccgcaa tgatataata tacataaagc ggttttctgc
     2221 caggcttttt attatcaagg ttcattggct tttcctccat ttcgttttgt tatgatgtac
     2281 catatcactt attcatctct ttttcaagaa aagcgagata aatttcataa agattttaga
     2341 attatctgaa ctgttcaata cggcaaataa aaaaaaagcg aaaaccttca caaacaaagg
     2401 cttcgcttac tttttagtgc cggcagtggg acttgaaccc acacgtggtt gcccacaaca
     2461 gattttgagt ctgcctcgtc tgccattccg acatgccggc attgaaacga gtattatcat
     2521 atcacgtttt tgtcgaaatg gcaagggcat ttttgagttt tttttaatat ttcacaaacc
     2581 tcgttttaga atacacattg cttgccttga tcagtttctg atatctggca aacagcttct
     2641 caggaagtct gatagttacg gttttgttga ccgcaaaagc tttttttcca acatagttca
     2701 gtgctgtact gttgatcctg atcactttga ctgccgggca ctggtaaaaa gctctggcat
     2761 ctatatgtct gacactggca ggaagtatga aatttcgcag ttttgtacac tgtgcaaatg
     2821 cgttgctgcc aattcttttt actctgctac caaatcttat gttccgcaga ttcgggcact
     2881 ggctgaacgc atattttcca atcgttgtta cattatttcc aaaaataaca tctgtaattt
     2941 ctttgttccc gctgaaggca ttcttgtcaa ttgtagttac tttataaact ttatcattga
     3001 tcacaactgt agccggaatc tgtatcacat tatattccgc agcaagacct gtaacgctta
     3061 cttctctttc attcaaaatc ttatagccaa ttccacctat caggtactta tagcttccac
     3121 cctgttcagt taacggagct gcattgaact gatatctcac aggttgtaca atatcgtttt
     3181 tctcccatat acctcctgta tattcttctc tctggaaaaa gacatatata ttgtatgcac
     3241 gttccacctt tgtataaata ccctttgcag aaccgatttc ccatttcctg tgaatatcgc
     3301 tttcctgcgg tttgagagaa aga
//
LOCUS       DBMDXG010000248         3322 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6492_length_3322_cov_1.393977, whole genome shotgun sequence.
ACCESSION   DBMDXG010000248 DBMDXG010000000
VERSION     DBMDXG010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6492_length_3322_cov_1.393977"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="ACZPD3_07255"
     CDS             <1..175
                     /locus_tag="ACZPD3_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR1415829.1"
                     /translation="HNHPSGDPTPSPDDLQITARIYQAGELLGIHLLDHIVIGDQRYC
                     SFREEGLWNTCTE"
     gene            198..1211
                     /locus_tag="ACZPD3_07260"
     CDS             198..1211
                     /locus_tag="ACZPD3_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR1415830.1"
                     /translation="MLFEKTYGIDLGSSSVKVYSFFRNKTYIEKNMIASKGHTIIAMG
                     NEAYDMFEKSPTDITVTSPMTFGMIANLELQEIVLYSMIRKIDHILGFGATMYFTVPL
                     DMTAVEKRAYFHVANGHWLKKNRVFMVEAPIADAIAMGVNLEDPEGNMIVNIGAQSTE
                     VSIITGGKIIISRKIPLGGRQMNESVCSEIRKRYNLQIGTRTAKRLKMVMGRLSDPKK
                     EVRKVVGIDCISGLPREEIISSYVVNDGIMNCLNEIAAEMKTFLERIPPQISYHIAKQ
                     GIYITGGSTRLPYIDKYLASYTGFTFNLSNFYETSAVTGLEKIIRNKELREWAVPVTQ
                     RKL"
     gene            1229..2140
                     /gene="mreC"
                     /locus_tag="ACZPD3_07265"
     CDS             1229..2140
                     /gene="mreC"
                     /locus_tag="ACZPD3_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR1415831.1"
                     /translation="MKNLKKKVHFRIKLKSKHLLVIMSIFCVSCIAATFSSGVTTAPL
                     QDAAGILIVPFENSINRISSVLKGMQDRMRDKEEILSENENLKAQIDSLTEQNNKLIQ
                     DQTEYVRLQQMYNLDQQYTEYPKIAAEIISKDPGNWYDTFMINRGSADGIRVDNNVLA
                     DKGLVGIVTEVGTHWATVRSIIDDSSNVSAMTVSTQDNCVVEGDLELIDEGKLSFSQL
                     YDQNNKVTVGERIVTSNISEKYVEGLFIGYVSEVEQNSNNLTKTGTIVTPVDFQHLKN
                     VLVITVNKQDSVNDNAQAAQEATANEE"
     gene            2130..2660
                     /gene="mreD"
                     /locus_tag="ACZPD3_07270"
     CDS             2130..2660
                     /gene="mreD"
                     /locus_tag="ACZPD3_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR1415832.1"
                     /translation="MKSKIALFFMILISFLLQCTLLHVISIGSITPNLILILCISMGL
                     MRGRKSGLWTGFFCGFLVDMFYGSVFGFYALIYMYIGFLSGYAHRICYDDDIKVPVML
                     AGIGDLLYGLSVYALQFLLRGRLGLGTYLYRIILPEIFYTVILTLIVYRVFHYINYHF
                     MKNPSMKESESIWVLK"
     gene            2645..>3322
                     /locus_tag="ACZPD3_07275"
     CDS             2645..>3322
                     /locus_tag="ACZPD3_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1415833.1"
                     /translation="MGTKIKRFFKKIRIKRTTILVLVFVFMSATLVRQLFELQIIQGE
                     AYISKFESRTTKKRVIKSTRGNIYDRNGEELATNVLAYSVTFEDNGTYDSTREKNLTL
                     NGIAYKVLKILESNGDSISTGFHIVLDESGNYAFDVDEGFTLNRFRADIYGEPLIDNL
                     TKKQKNATADQMIEFMSGKEGFSIVLYGDNAYTKEELTSHGLPENLSKQDILELSKIR
                     YALSTNSF"
BASE COUNT         1039 a          609 c          769 g          905 t
ORIGIN      
        1 acacaatcat ccaagtggtg atcctacacc aagtccggat gacttacaga tcacagccag
       61 gatttatcag gcgggggaac tgctgggaat acatctgctg gatcatattg tgatcgggga
      121 tcagagatac tgcagcttca gagaagaagg attatggaat acatgtacag aataaggcaa
      181 caacaaaggg gcagaatatg ctatttgaga aaacatatgg aatagatttg ggaagcagtt
      241 ccgtaaaagt ctattctttt ttcagaaaca aaacttatat cgagaaaaat atgatcgcgt
      301 ccaagggaca cacgatcatt gcaatgggaa atgaagcata cgatatgttt gagaaatccc
      361 ccacagatat tacggttact tcgccaatga catttggcat gatcgctaat ctggaacttc
      421 aggagattgt tctttacagt atgatccgaa agatcgatca tatcctggga tttggcgcaa
      481 caatgtattt tactgttcct ctggatatga ctgcggttga gaaacgggca tattttcatg
      541 tggcgaatgg tcactggtta aagaaaaatc gcgtatttat ggtagaggca cccattgcag
      601 atgccattgc catgggtgtc aatctggaag atccggaagg aaatatgatc gtaaatatcg
      661 gtgcacagag tacagaagta tcgatcatca ccggaggaaa gatcattatc agcaggaaaa
      721 taccgctggg aggcagacag atgaatgaat ctgtctgcag tgaaatacgt aaaagatata
      781 atctgcagat tggcacaagg actgccaaga ggcttaagat ggtcatggga cgcctcagtg
      841 atccgaaaaa ggaagtacgt aaggttgtag gaattgactg tatttccgga ctgccaagag
      901 aagaaatcat ttcttcttat gtggtaaatg acggtatcat gaactgtctg aatgagattg
      961 cggcagagat gaaaacattt ctggagcgaa ttcctcccca gatttcctac catattgcga
     1021 aacaggggat ttacataacc ggaggcagta ccagacttcc ttatattgat aaataccttg
     1081 ccagttatac aggattcacc tttaatctgt caaattttta tgaaacctcg gcagttacag
     1141 gtctggagaa aataatcaga aataaagaac tgcgtgaatg ggcagtaccg gttacgcaga
     1201 gaaaactata atttttaggg gtgcatcaat gaaaaacctg aaaaagaaag tacattttag
     1261 gattaaatta aaaagtaaac atcttctggt cattatgtcg attttctgcg tcagctgtat
     1321 tgctgccact ttttcatccg gcgtaaccac tgcaccgctg caggatgccg ccgggatact
     1381 gatcgttcca tttgaaaaca gcatcaatcg gatcagttca gttctgaaag gaatgcagga
     1441 ccgtatgcgt gataaagaag aaatcctcag tgagaacgaa aatttaaagg cacagataga
     1501 cagtctcaca gaacagaaca ataaactgat ccaggatcag acagaatatg taagattaca
     1561 gcagatgtat aatcttgacc agcagtatac cgaatatcca aagattgctg cagaaatcat
     1621 atccaaagat ccgggcaact ggtatgatac ctttatgatc aaccggggat ctgcagacgg
     1681 cattcgtgtg gataataatg tacttgcaga taaaggtctg gtcgggatcg tgacagaggt
     1741 cggaactcac tgggcgactg tacgttccat tatcgatgac agcagcaatg tcagtgccat
     1801 gactgtcagc acacaggata actgtgtggt ggaaggagat ctggaactca tcgatgaggg
     1861 taaattaagc ttcagccagt tgtatgacca gaataataaa gtaacagtgg gggaacgcat
     1921 cgttacctcc aatatcagtg agaaatatgt agagggactg tttatcggat acgtcagtga
     1981 ggtggaacaa aacagcaaca atctgaccaa aacaggtacc attgtcacac ctgtagattt
     2041 ccagcatctg aaaaatgtac ttgtcattac agtaaataaa caggattctg ttaatgacaa
     2101 tgctcaggca gctcaggagg cgactgcaaa tgaagagtaa gatagcgtta ttttttatga
     2161 tcctgatcag ttttcttctt cagtgtactc ttcttcatgt gatatccatc ggttctatca
     2221 cgccgaatct gatcctgatc ctgtgtatat ccatggggct gatgcgcgga agaaaaagtg
     2281 gactgtggac aggatttttc tgtggatttc tggtagacat gttctatggc tctgtgtttg
     2341 gtttctacgc tctgatctat atgtatattg gctttttaag cggatatgcc cacaggatct
     2401 gctatgatga tgacataaag gttccggtta tgcttgcagg tatcggggat ttactgtacg
     2461 gactttctgt ctatgcattg cagtttctgc tcaggggacg tctggggctc ggaacctatt
     2521 tataccggat tattttaccg gagatttttt atacagtgat ccttacactg attgtttacc
     2581 gggtatttca ttatattaat tatcatttca tgaagaatcc atcaatgaag gaaagtgagt
     2641 ctatttgggt actaaaataa aaagattttt taaaaagata cgaataaaac gtaccacaat
     2701 actggtactg gtttttgtat ttatgtcagc cactctggtc cgtcagcttt ttgaactgca
     2761 gatcatccag ggagaggctt atatcagcaa atttgaatca agaacaacaa aaaaacgtgt
     2821 gatcaaaagt acgcgtggca atatctatga cagaaatggt gaggagctgg caacaaatgt
     2881 gctggcttat tctgttacct ttgaggataa tggaacttat gattctacca gagaaaagaa
     2941 tcttacactg aacgggattg cctataaagt cctgaaaatc cttgagtcca acggggattc
     3001 catcagtacc ggcttccata ttgttttgga tgagagcggg aattatgctt ttgatgttga
     3061 tgagggattt actctgaacc gttttcgtgc ggatatttac ggagaaccgt tgattgataa
     3121 cctgacaaaa aaacagaaaa atgccactgc agatcagatg atcgagttta tgagcggaaa
     3181 agagggattt tccattgttc tttacggcga taatgcttat accaaagaag aactgacatc
     3241 ccatggactt ccggagaacc tgtcaaagca ggatatactt gagctttcca aaatccgcta
     3301 tgcactgagc actaacagtt tc
//
LOCUS       DBMDXG010000249         3309 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6518_length_3309_cov_1.914277, whole genome shotgun sequence.
ACCESSION   DBMDXG010000249 DBMDXG010000000
VERSION     DBMDXG010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6518_length_3309_cov_1.914277"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="ACZPD3_07280"
     CDS             <1..560
                     /locus_tag="ACZPD3_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1415834.1"
                     /translation="TKTVKVGNTIYYCTEKGILEAWKKGKTIYYPNGKKMNSTKAYEY
                     ETLQRAKDVVSKITKPSMSKSEKFETCFRWVMYQHYYDTRRIFYNQTAWPALYANDYL
                     ILSGKGGDCFSDACSFAYLAKALGYKNVYVCVDTTATDGSGHCWAEIGGRVYDPLFAE
                     AKSYYGYFGVGYGSYGLYPERRVAV"
     gene            complement(681..1343)
                     /gene="trhA"
                     /locus_tag="ACZPD3_07285"
     CDS             complement(681..1343)
                     /gene="trhA"
                     /locus_tag="ACZPD3_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR1415835.1"
                     /translation="MKFKLKDPGSAITHGIALLLAAVGAVPLIIKAARSYDVLHIVAL
                     SIFILTMVLLYAASTIYHSVDSTEKVNRRLRKMDHMMIFVMIAGSYTPVCLIVLHNRI
                     GYILCALVWSIAVLGIILKGCWITCPKWLSSVLYIAMGWLCVLAFVPIFHALPRAGFD
                     WLLAGGIIYTIGGVIYALKVPLFNSRHKNFGSHEIFHIFVMLGSACHFIVMYFFVAPL
                     PV"
     gene            1593..2381
                     /locus_tag="ACZPD3_07290"
     CDS             1593..2381
                     /locus_tag="ACZPD3_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1415836.1"
                     /translation="MGKTITICFTNNKGGSGKTTTCSNLGAAMAQAGKKVLLIDGDMQ
                     LNLSLAFFPENWVLEQAKGEKNLYCAIGKQEELSGYVVHTPYENLDLIPSSTLMSSIE
                     YELFTKWQREFILRKCIQKIKDAGNYDYILIDAPPTLGGWVMNILCASDRVIIPVEAS
                     PWGMFGLANMFEFLNEVKQITPELEVAGIAVTKVDTRKSYYKQTMETLHELEDIHVFE
                     QIIRVDSSVEWSQDNSIPVVEYKKSSRSAREYTRLAEEVMELCQ"
     gene            2372..2557
                     /locus_tag="ACZPD3_07295"
     CDS             2372..2557
                     /locus_tag="ACZPD3_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415837.1"
                     /translation="MSVGRESIRRAANAGAGKTVKKPVVSTKEAEKPETISVTEEKKK
                     TAKGSVHINEELPVYLL"
     gene            2636..>3309
                     /locus_tag="ACZPD3_07300"
     CDS             2636..>3309
                     /locus_tag="ACZPD3_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1415838.1"
                     /translation="METEEKKIYTIEDIARELGVSKTTVSRAISGKGRISQATRDRVR
                     AFIKEHDYRPNVVAKGLAQRKTYNIALLMPKDYVATEFLFFKDCMNGICEMASSYEYD
                     IIISMIDGADVSQIQRLEANRKVDGIIVSRAVVSSKVQKYLKNCKEPYILIGPSSDPE
                     VPFVDNKNQEAGKELTSIMLMKGFRNLALLGGNQSYNVTGSRYQGFLEAHEEMGVSVN
                     ENLIFM"
BASE COUNT         1136 a          538 c          796 g          839 t
ORIGIN      
        1 gaacgaaaac agttaaagtt ggaaatacaa tttattattg tacagaaaag ggaattctgg
       61 aagcctggaa aaaaggaaag acgatttatt atccaaatgg aaagaaaatg aattccacca
      121 aagcgtatga gtatgagact ttgcagagag caaaggatgt tgtcagcaag atcacaaaac
      181 cgagtatgtc aaaaagtgag aaatttgaaa cctgtttcag atgggtaatg tatcagcatt
      241 attacgatac cagaagaata ttttataatc agactgcatg gcctgcgtta tatgcaaacg
      301 attatctgat actgagcgga aaaggcgggg actgtttctc agatgcctgt tcgtttgcct
      361 atcttgcaaa ggctcttgga tataagaatg tttatgtctg cgtagataca acggcaactg
      421 acggaagcgg tcattgctgg gctgaaatcg gaggcagagt atatgatccg ctgttcgcag
      481 aggcgaagag ctattatgga tattttggcg tgggatatgg atcctatgga ttgtatccgg
      541 aaagaagagt agctgtgtaa tataaaagct ttcctgcagg gattatgctg cagcagagaa
      601 aattgtattg ttaaggggtt gtagcaccgg aaaaaatccg gtgccgcaac cccttgtttt
      661 gtttattggc aaattgacgg ttatacaggt agtggagcaa caaaaaagta catgacaatg
      721 aaatggcagg cacttccaag cataacaaag atgtgaaaga tctcatggga accaaaattc
      781 ttatgtctgg aattaaaaag cggaactttt aatgcataga tcacaccacc gatggtataa
      841 atgattccgc ctgcaagaag ccagtcaaat cctgcacgtg ggagtgcatg gaagattgga
      901 acaaatgcca gtacacacag ccagcccatg gcgatataca gtacagagga caaccatttg
      961 ggacaggtaa tccaacagcc ctttaagata atgccgagga ctgcaatact ccataccagc
     1021 gcgcacagaa tatatccaat gcggttatga agaacgatca ggcatacagg agtatagctt
     1081 ccggcgatca tcacaaaaat catcatgtga tccatttttc gcagccgtcg attgaccttc
     1141 tctgtagaat ctacagaatg gtaaatggta ctggctgcat agagcagaac catggtaagg
     1201 ataaaaatac ttaaagcaac aatatggagc acatcataag aacgcgctgc cttgataata
     1261 agaggaactg ctccgactgc agcgagaaga agggcaatac catgagtgat cgcacttccg
     1321 ggatctttta atttaaattt cataaaaacg cctccattca ttcagataaa tataatgaaa
     1381 acaatataac atagttttaa aaactacata tcaagtatat aaatactata gcacaaaaaa
     1441 acaaaaaatc aagggcttat tttgggaatc aaagaaatgt ttgtccggca atgcaaatga
     1501 gttacagggt tgtgaagatg aggaaaacag ttgaatctat atgataaatc tcctataata
     1561 caagtaaagt aagtggtaaa ggagacagtt ccatgggaaa aaccataacg atatgtttta
     1621 caaataataa gggtggaagt ggaaaaacca caacctgttc aaaccttgga gcggcgatgg
     1681 ctcaggcagg gaaaaaggtt cttctgatcg atggagatat gcagttaaat ctttctcttg
     1741 ctttttttcc ggagaactgg gtgctggagc aggcgaaagg agagaagaat ctgtattgcg
     1801 caatcggaaa acaggaagag ctcagcggtt atgtggtaca tactccctac gaaaatctgg
     1861 atctgatacc atcgtctaca ttgatgagtt ctatagaata tgaacttttt acaaaatggc
     1921 agagagaatt tatcctgcga aaatgcattc agaagataaa ggatgcagga aattatgact
     1981 atattctgat tgatgcacct cctacgctgg gtggttgggt gatgaatatc ctttgtgcat
     2041 cagaccgggt aattattcct gttgaggcaa gtccatgggg aatgttcgga cttgcgaata
     2101 tgtttgaatt tcttaatgaa gtaaagcaga ttactccgga actggaagtt gccggaattg
     2161 cagtgacaaa ggtagataca agaaagagtt attataaaca gacaatggaa acactgcatg
     2221 aacttgagga tattcatgta tttgagcaga tcatcagagt ggacagctct gtagaatggt
     2281 cacaggataa cagtattccg gttgtggaat ataaaaaatc aagcagaagt gccagggaat
     2341 atacgagatt ggcagaggag gtcatggaat tatgtcagta ggcagggaga gtattcggcg
     2401 tgcagcaaat gcaggtgccg ggaaaaccgt aaagaagccc gttgtatcca caaaagaggc
     2461 agagaaaccg gaaacaatat ctgttacaga agaaaagaaa aaaacagcaa aaggttctgt
     2521 tcatattaat gaggaactgc cggtatatct tctttaaaag ttacttgtaa gtgcttgagt
     2581 attttgttat tttatagaat ataatcaagt ggttgcgcaa aaagtggggt attttatgga
     2641 aacagaagaa aagaagatat atacaataga agatatcgcc cgggagcttg gggtaagtaa
     2701 gactacggta tcacgtgcta tttcaggaaa gggaagaatc agtcaggcta caagagacag
     2761 agtacgggca tttataaaag aacatgatta cagacccaat gttgtagcaa aggggttggc
     2821 acagcgtaaa acttataata ttgcactgct gatgccgaag gattatgttg ccacagagtt
     2881 tctttttttt aaagactgta tgaatggaat ctgtgagatg gcttcctcct atgagtatga
     2941 tattattatt tctatgattg atggagcaga tgtatcgcag atacagagac tggaggcaaa
     3001 tcgaaaagtc gatgggatca ttgtaagcag agcagtggta agttccaagg tacagaaata
     3061 tctgaagaac tgtaaggaac catatatatt gatcggccca tcttcagatc cggaagttcc
     3121 gtttgtagat aacaagaacc aggaagccgg aaaagaactg acaagtatta tgttgatgaa
     3181 aggttttcgg aatctggcac ttctgggtgg aaatcagtcc tacaatgtta ccggaagcag
     3241 atatcagggt tttctggaag cacatgagga aatgggagtt tctgttaacg aaaatctgat
     3301 cttcatgga
//
LOCUS       DBMDXG010000250         3296 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6547_length_3296_cov_2.354304, whole genome shotgun sequence.
ACCESSION   DBMDXG010000250 DBMDXG010000000
VERSION     DBMDXG010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6547_length_3296_cov_2.354304"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="ACZPD3_07305"
     CDS             <1..418
                     /locus_tag="ACZPD3_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR1415839.1"
                     /translation="KGKVLETLKTDAAGHAESKLYEIATFKNGKYDTAIKYYLVETKT
                     LDGYTLDQTKHEVTFAYADDSTPVVEVTFNLTNEKPEVPVTPNTPDIPQSHEETKVSN
                     APKTGDSTNIWLPILLLVISAGGMAGLYISRKRKSK"
     gene            506..646
                     /locus_tag="ACZPD3_07310"
     CDS             506..646
                     /locus_tag="ACZPD3_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415840.1"
                     /translation="MMKTMKKRQEARKNQEKTVKRMMAYQKKAEQMVSRKRMNHHMIR
                     KG"
     gene            653..1741
                     /locus_tag="ACZPD3_07315"
     CDS             653..1741
                     /locus_tag="ACZPD3_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HIR1415841.1"
                     /translation="MMNRKEFYEYVKDNVKEYLPESYKDAEIKLQEVEKNNGLKLTGI
                     TIPNGDQRIVPTVYLDSLYLEYVIGKDVDSCVGDVVDMYIEAQDKAEFIKMGVPDIFD
                     YEKMKEKLQVRICDKEWNTDRLADKVVTEHGDFAAYYAVNLEENGEGISSIPVTVSLM
                     NEWGVSVEQIQADAMMADKNRGVQLVDMTQIVESMIFGGTPKNLLNEKLDMETVENPM
                     FCLTNESKMNGASLLLQEDIRKQIGECLGSDYFVIPSSVHEVLILPDNGIFQVPELNA
                     MVKEVNETQVEREEQLSDKVQFCDKKTAVMENAERREARLEKEKAAEKAEVKGGIHGR
                     LEKAKAEIKAKESDKVPKNKSKDLSTAL"
     gene            1810..>3296
                     /locus_tag="ACZPD3_07320"
     CDS             1810..>3296
                     /locus_tag="ACZPD3_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR1415842.1"
                     /translation="MANKLYAMEQLTEEVAKDVAASPQEWMRFLNTASRLYKYTFPEQ
                     LLIYAQRPEATAVASMEIWNQKMYRWIKKGSKGIALIDNTSGPKTKLRYVFDVQDTYK
                     VRNLGKDPQLWNLPMEGEQLVADYLQEQLSLEDTEGGLAESLHQAAKESMQEWLPDAL
                     EELRLDVTGTFLEELDEQNQEVEFRELMTNSVWYVLLNRCGLDAQEYLDAEDFRHITD
                     FNQLKVLGHLGSAVNEISRPVLMQIGRYVLNDLENDLKTVAKEKEVAYNEFNTLIRES
                     NTDNTEDREEKKEETDYERDQLQPERRVSDSRYQPGRDERNHREVRNDEERVSEKPQG
                     SQVQHSDTAEPSGQSPDGDRQSGKAESRQPDERTSGERSGTGQNGRRDGMDQTHEPDQ
                     GTGRGAGDSGDYLQLSLFPTEEEQLGEIRKAAAALEQPAAFLISDEVVNDILRTGSGQ
                     KNTLFHITARLIEGLDNEEMRSFLKDEYGTGGKGFTIDGQKISIW"
BASE COUNT         1194 a          548 c          841 g          713 t
ORIGIN      
        1 caaaggaaaa gtactggaaa ccttaaagac agatgctgcc ggtcatgcag aaagtaagct
       61 gtatgagatt gccacattta agaacggcaa atatgatacc gctatcaagt attatctggt
      121 ggaaacaaag acactggacg gatacacact ggatcagacc aagcatgaag tgacatttgc
      181 ctatgcagat gacagtacac cggttgtaga agttaccttt aatctgacaa atgagaaacc
      241 ggaagttcca gtaacaccga acacaccgga tataccacag tcccatgaag agacaaaggt
      301 atcaaacgca ccgaaaacag gtgacagcac gaatatctgg ctgccaatcc tgcttcttgt
      361 aatctccgca ggaggtatgg caggacttta tatcagcagg aaaaggaaaa gcaaataagg
      421 aaactaaaac caacggttat gattgggagc ttctatgtga ggctcccttt ttcatacaaa
      481 aaatcacaga aaggaacaga aaactatgat gaaaacaatg aaaaaaagac aggaagcacg
      541 aaaaaaccag gagaaaacag taaagagaat gatggcttat cagaagaaag ctgagcagat
      601 ggtcagcaga aaaagaatga accatcacat gatcagaaag gggtaaaagc gtatgatgaa
      661 cagaaaagaa ttttatgaat atgtcaaaga caatgtaaag gagtatctgc cagagtctta
      721 caaggacgca gaaattaaac tgcaggaagt agaaaaaaac aacggtttga agttgacagg
      781 tattacgatt ccaaacggag atcagagaat cgtaccgaca gtctatctgg attccctcta
      841 cctggaatat gtgattggaa aagatgtgga ttcttgtgtt ggcgatgtag ttgatatgta
      901 tattgaagca caggataaag cagaatttat taaaatggga gtacctgaca tttttgatta
      961 tgaaaaaatg aaggaaaaac tgcaggtgag aatctgtgac aaggaatgga ataccgatcg
     1021 tctggcagat aaagtagtca cagaacacgg agattttgca gcttattatg cagtaaatct
     1081 ggaggaaaat ggagagggaa tcagcagtat tccagtaact gtttctctga tgaatgaatg
     1141 gggagtatct gtcgaacaga ttcaggcaga tgcgatgatg gcagataaga acagaggtgt
     1201 gcaactggtg gatatgacac agattgtcga atccatgatt tttggaggaa caccaaagaa
     1261 tcttttgaat gaaaaactgg atatggaaac tgtggagaat ccaatgttct gccttaccaa
     1321 tgaatctaag atgaatgggg cttccctgtt actgcaggaa gatattcgta aacagattgg
     1381 ggaatgcctg ggaagtgatt actttgtcat cccatcttcc gttcatgaag tgttgattct
     1441 ccctgataac ggaatttttc aggttccaga attaaatgcg atggtaaaag aagtcaatga
     1501 gacacaggta gaaagagagg aacagctgtc agataaggtt cagttctgtg ataaaaagac
     1561 agcagtaatg gaaaatgctg agcgcaggga agcacgtctg gagaaagaaa aagcagctga
     1621 aaaagcggaa gtaaaaggtg gaatccatgg aagattggag aaagctaagg cagaaatcaa
     1681 ggcaaaagag tcagacaaag tgccaaagaa taagtcaaaa gatctttcca cagcattata
     1741 aagagtaaca atgatgattt taaggggact gtgccaagca gtcccttatt ttatgaaaag
     1801 gagtagcaca tggcaaataa attatatgca atggaacagc tgacggaaga ggtggcaaaa
     1861 gatgtagctg ccagtccgca ggaatggatg cggttcctaa acacggcatc cagactgtac
     1921 aaatacactt ttccagaaca gcttttaatt tatgcacagc ggccggaagc aactgccgta
     1981 gcatccatgg aaatctggaa tcagaagatg taccgctgga tcaaaaaggg atctaagggg
     2041 attgccttga ttgacaatac ttccggaccg aaaacaaaac ttcggtatgt atttgatgtg
     2101 caggacactt ataaagtgcg gaatctggga aaagatccac agttatggaa tcttccaatg
     2161 gaaggtgaac aactggttgc agattatctg caggagcagt tatcactgga agatacagaa
     2221 ggaggacttg ctgaaagtct gcatcaggca gcgaaggaga gtatgcagga atggcttcca
     2281 gatgcattgg aagaactgag actggatgta acagggacat ttttggaaga actggacgaa
     2341 cagaatcagg aagtagaatt ccgggaactg atgaccaata gtgtctggta tgtattacta
     2401 aaccgatgtg ggctggatgc acaggaatat ctggatgcag aggattttcg gcatattacg
     2461 gacttcaatc agctgaaagt attaggacat ctgggaagtg cagtcaacga gatcagcaga
     2521 ccagtcctga tgcagattgg acgatatgtt ctgaatgatc tggaaaatga cctgaaaacg
     2581 gttgcaaaag aaaaagaagt agcttataat gaatttaaca cattaattcg tgaaagcaat
     2641 acagataaca cagaagatag agaagaaaaa aaggaggaaa cagactatga gagagatcag
     2701 ttacagccag aacggagagt atcagattcc agatatcagc ctggaagaga cgagaggaac
     2761 catcgggaag tacggaatga tgaggaaaga gtatctgaga aaccacaagg tagccaggtt
     2821 caacattctg acactgcaga accgtctgga cagtcacctg atggagatcg acagtcaggc
     2881 aaggcagaga gtagacaacc tgatgaacga acttctggag aaagatccgg caccggacaa
     2941 aatggcagac gggatggcat ggaccagaca catgaaccag atcaaggcac aggcagagga
     3001 gctggtgatt caggagatta tttacagtta agcctgttcc caacagaaga ggaacagtta
     3061 ggagaaataa ggaaagcggc agctgcattg gaacagccag ccgcttttct tatttccgat
     3121 gaagtggtaa atgatattct ccgcacggga agcggacaga aaaatacact gtttcatatt
     3181 acagcaagac tgattgaagg tctggataat gaagaaatgc ggagttttct gaaagatgaa
     3241 tatggaactg gtggcaaagg atttaccata gatggtcaga aaatcagtat ctggta
//
LOCUS       DBMDXG010000251         3296 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6549_length_3296_cov_1.250391, whole genome shotgun sequence.
ACCESSION   DBMDXG010000251 DBMDXG010000000
VERSION     DBMDXG010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6549_length_3296_cov_1.250391"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="ACZPD3_07325"
     CDS             <1..732
                     /locus_tag="ACZPD3_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HIR1415843.1"
                     /translation="SETASYEAIVPFINMNYDGLKNAIIEILSGASIKVNTAAFKNDT
                     VNIQSKDDVLTYLIHLGYLGYNQNRRTAFVPNEEIRQELTMAVESRKWNEMITFQQES
                     EHLLEATLDMDEEAVEEGIEKIHTEYVSNIQYNNENSLSSVLAIAYLSSMEYYFKPVR
                     ELPTGRGFADFVFIPKPEYVSSYPALVVELKWNKNVKTALQQIKEKQYPDSVLDYTGD
                     ILLVEINYDKKTKEHQCLIEKYEKL"
     gene            782..1546
                     /locus_tag="ACZPD3_07330"
     CDS             782..1546
                     /locus_tag="ACZPD3_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR1415844.1"
                     /translation="MELKEKTPHTILNEIIEAAKECGQVMLQADRANFGIKDKAGKAN
                     FVTKYDCKIQEMLEKKLSEILPEAEFWGEEEDCQINRNAEYIFVVDPIDGTTNFIKDY
                     HMSCVSIGLIRNGKRYMGVVHNPYLNETFYAISGEGAYMNGNAIHVSKDDLANSIVLF
                     GSSPYNTELAKASFELAYEYFQKCLDIRRSGSAALDLCAIASGRAEIYFELILSPWDF
                     AAGALIVEEAGGIVTTVEGEELPCLEKSSILARNKQ"
     gene            1715..2068
                     /locus_tag="ACZPD3_07335"
                     /pseudo
     CDS             1715..2068
                     /locus_tag="ACZPD3_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703977.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2148..3170
                     /locus_tag="ACZPD3_07340"
     CDS             2148..3170
                     /locus_tag="ACZPD3_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR1415845.1"
                     /translation="MEIIHENEYVYKMNDEIEPYLATHRQEGYIPGAEDHLHRKDMGI
                     VGKIHVQRYLAEKPKGVIIISHGFTEAAPKYDEMIYYFLKAGYHVYMPEHMGHGQSYR
                     LTADSSLVHIDTWKRYVRDFLKICHVIKKTYPELPLVLFAHSMGGAIGTIAAAWEPQL
                     FQKIILNSPMLRPLTGNVPWPLVIAIAQTKCLLGREEDYVAGQKPYDGGETFETSGCT
                     SRPRFEKYNELCKENKKFQVSAASYGWLLASVKMSWYIKYCGWKKLIAPVLLFQAERD
                     DFVSVNALRKFAEKINHRGITSCEYVYLPGTRHETYRSDDRTMEMYLDKIMKFMDSGM
                     VSDSCT"
BASE COUNT         1047 a          492 c          807 g          950 t
ORIGIN      
        1 tctgagactg cttcttatga agcaattgtt ccttttatca atatgaatta tgatggtttg
       61 aaaaacgcta taattgaaat actttccggt gcttcgatta aagtgaatac agctgctttc
      121 aaaaatgata cggtgaatat acaaagcaaa gatgatgtac ttacgtattt gatccatctt
      181 ggttatttgg gatataacca gaatagaaga actgcatttg ttccaaatga agaaatccgg
      241 caggaattga caatggcggt tgaaagcaga aaatggaatg agatgattac ttttcagcag
      301 gaatcagaac atttactgga ggctacgctg gacatggatg aagaagcagt tgaagaggga
      361 attgagaaaa tccatacaga gtatgtttcg aatattcagt ataataatga gaattctttg
      421 agcagtgtac ttgcaattgc atatctgagt tcaatggaat attattttaa accagttcgt
      481 gaactgccga caggacgtgg gtttgcagac tttgtattta ttccaaaacc agaatacgtt
      541 tccagttatc cggcgcttgt cgtagaactt aaatggaata agaatgtcaa gacagcatta
      601 cagcagatta aggaaaaaca atatccggat tcagttctgg attataccgg agatattttg
      661 ctggttgaga taaactatga caagaagaca aaagaacatc agtgtctgat tgaaaagtat
      721 gagaaattat aaaagtattg ataaaacgga aaccattttc tattgagagg tgacagataa
      781 tatggaacta aaggagaaaa ctccacatac gatattaaat gaaataattg aagcggcaaa
      841 agaatgcgga caggttatgc ttcaggcgga cagggcgaat tttggcatta aggataaggc
      901 aggaaaagca aattttgtta cgaagtatga ttgcaaaatt caggaaatgc tagaaaaaaa
      961 gttgtctgag attcttccgg aagcggagtt ttggggggaa gaagaggatt gccagattaa
     1021 cagaaatgca gaatatattt ttgtggtgga tccgattgat ggaactacca attttattaa
     1081 agattatcat atgagctgtg tatctattgg attgattcga aatggaaaga gatatatggg
     1141 agtcgtgcat aatccgtatc ttaatgaaac tttctatgcg atttccggtg agggtgcata
     1201 tatgaatgga aatgcgattc atgtatcgaa ggatgatctg gcaaacagca ttgttttgtt
     1261 tggttcatca ccgtataata cggagcttgc gaaggcatcc tttgaattag cttacgaata
     1321 ttttcaaaaa tgccttgata tccgacgaag cggatctgca gcacttgatt tatgtgccat
     1381 agcatcagga agagcggaaa tttatttcga gcttatttta tctccatggg attttgcggc
     1441 aggtgcactt attgttgaag aagcaggcgg aattgtgacg actgttgaag gagaagaact
     1501 tccatgcctg gaaaagagtt cgattcttgc aagaaataag cagtagttct gaaatctgga
     1561 ggtgcacggt gaaaaagtta acaacaggga aaatatggca gtttgcagcc ggacagtttg
     1621 gatgggcgat gttgtccggg attatttcta actgatttcc tttacattta ttgccggaac
     1681 tgcagtggcg tatctggcac ctacgatatg ggggctgttc attccggcat cggtgcaggg
     1741 actgatcctg acagtcgtgg cggcccttcc tatggcgatt ttcggaattc ttccgcaggc
     1801 tgttgtggca gatatttcgc agagcgactc gattacatca ggaagcaatc gtgaaggaat
     1861 gttttatgcg gcgagaacat ttgcgtttaa acttgggcag agtatttcta tgttgatatt
     1921 tacagcagtc tcgacgatag gggcagcaga gggaactggt tatagagttg ctgcttttgg
     1981 ggcagcagta ttctgttgta taggcggaat tatactggta ttttataatg agaaaaagat
     2041 taatgggatt attaataggc accagtgaag attataatac aggtatgtat cgaatggtga
     2101 aaagcagata gcaatagaat tatgaaaaag taacaggagt gtatacaatg gaaataatac
     2161 atgagaatga atatgtgtat aaaatgaatg atgaaataga gccatatctt gcaacgcacc
     2221 gtcaggaggg atacattcct ggtgcagagg atcatctgca ccgaaaagat atggggatag
     2281 tggggaaaat tcatgtacag cgttacctgg cagagaagcc gaaaggcgtt attataatca
     2341 gtcatggctt tacagaagca gcgccgaaat atgatgagat gatttattat tttctgaagg
     2401 ctggatatca tgtgtatatg ccggaacata tgggacatgg acaaagttat cgtctgacag
     2461 cagattcatc tcttgtacat atagatacct ggaagcgata tgtgagagat tttttgaaaa
     2521 tatgccatgt aattaaaaaa acatatcctg aactgccgtt agttcttttt gcgcattcta
     2581 tgggtggggc gattggcaca attgcagctg catgggagcc acaactgttt cagaagatta
     2641 tattgaattc tcctatgctc cgcccgctta ccggaaatgt tccctggcca ttggtgattg
     2701 ctattgcaca gacgaagtgc cttttgggaa gggaggaaga ctatgtggct ggtcagaagc
     2761 cttatgatgg tggtgagact ttcgaaacca gtggatgtac ttccagaccg agattcgaaa
     2821 aatataatga gctgtgtaaa gagaataaaa aattccaggt tagtgcagct tcttatgggt
     2881 ggcttctggc gtctgtaaaa atgagttggt acataaaata ttgtggatgg aagaaactga
     2941 tagcacctgt tttgctattt caggcagaaa gagatgattt tgtatctgta aacgcattga
     3001 gaaaatttgc agagaaaatt aatcatagag gaattacttc ctgcgaatat gtttacctgc
     3061 cgggaaccag acatgagacc taccgcagcg atgacaggac gatggaaatg tatttagata
     3121 agattatgaa gtttatggat tcaggcatgg tatctgacag ttgtacgtga ttcgtatctt
     3181 aatgaaattt tctatgcgat tcatgtatcg gaggataatc aaataatgga aatggttttc
     3241 tgttatcagc atctctacta agagaactga taacaggaaa ccatttttat tatttg
//
LOCUS       DBMDXG010000252         3271 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6613_length_3271_cov_2.043218, whole genome shotgun sequence.
ACCESSION   DBMDXG010000252 DBMDXG010000000
VERSION     DBMDXG010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6613_length_3271_cov_2.043218"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            38..544
                     /locus_tag="ACZPD3_07345"
     CDS             38..544
                     /locus_tag="ACZPD3_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415846.1"
                     /translation="MELLSQFMDELERTESREQYDRILGKIVVVGTYLKRRKNLVLTQ
                     YASDGNLLTMEDLRQSLAESCDSLKLCRIRAAYYVENVDVQMNADDILKCYDTFEWLV
                     ERLLDIMQSVFYRVSQIDDALRISVHIVSEVDLRGVMSERPELKVQQEDENEWFISCI
                     VFRKRGGR"
     gene            541..1884
                     /locus_tag="ACZPD3_07350"
     CDS             541..1884
                     /locus_tag="ACZPD3_07350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1415847.1"
                     /translation="MIDFVECGMAMQDFLMILFEICLVLELILLLVRITCFSGKKKIV
                     TAVAVFAVSFMFMGVLMNDHRYCLGESFYQPMLQGYPFSVLMTVVIGLLLYLCWAIRS
                     ERRRYHHSLSYWSVNEAVNDVPCGVCFSDPLGRIVLCNTKMQELSRIMTGSYLQDYEA
                     LRKAMSGEPESEGLCRLSKDSNVFYFPDGSVWMFQEYGLQEPDCAGYLQTVAVNVSEV
                     YYNGEKIRANNEKLEILNHKLEEMYEKIGDKIREQETLVMKMQVHDNFGRSLLSIRRI
                     LERKEDPDKMDKQLSVLKHLVYILTGSAVESMEEVYEDTIRHAEELGISVQISGNFPM
                     HPSYRLLTDRAIRECVTNCARHAHGSTVWVKIDKNADEYTVQITNDGEVPDKNAEEGG
                     GLSALRKAAESGKCRMQVSFSPEFCLILKMPLTERMGFDGTDTDSRRSEDHAEVF"
     gene            1987..2457
                     /locus_tag="ACZPD3_07355"
     CDS             1987..2457
                     /locus_tag="ACZPD3_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HIR1415848.1"
                     /translation="MDVQTFHNHDGLSAGKEIREKYPYTKVLIVTSLIDPKVLERAKS
                     GCADSLWYKDHGEEEIRSVIHRTLNGEHVFPDMTPKVELNWITSGDISPRQLEMLRLY
                     IRGMSYSEIAREMKCSTSGVRWNFQEMIARAGYSCKEDLIAAALESKLIVTTLK"
     gene            2546..>3271
                     /locus_tag="ACZPD3_07360"
     CDS             2546..>3271
                     /locus_tag="ACZPD3_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415849.1"
                     /translation="MKKYSVAVCVCAVLFSVGIHGGGMREVSARAPGADENLAILQDI
                     IRENECQAGMAFLGYTYEGAEAAEILEYTRQGEVAAAYPFLQDGTVVDAGGYEIYAIV
                     PGDDCRVSVYPAQPTEEGEYLDDLNAPYYTGKENEIIILRCNVSEIHSNVMVSVQKQN
                     MSIQYHPMVSLKDGHLAAETHCYDFSIYYNWDEYDPEYNPVYESEYDQECNPEHDSAL
                     NIKIAREQLSETDEVSYYLGLGMS"
BASE COUNT          974 a          521 c          886 g          890 t
ORIGIN      
        1 taaaattcaa cgagcaggtt gaggaagcta gggcagagtg gaattgctgt cacagtttat
       61 ggatgagctg gaacggacag aatcaaggga acaatatgat cggattctcg gtaaaattgt
      121 agttgttggt acgtatttga agcggagaaa gaatctggtg ctgacacaat atgcatcaga
      181 tggaaatctg cttacaatgg aagatctcag acaaagcctg gcggaatcct gtgacagctt
      241 aaaattatgc aggatccggg ctgcttacta cgtggaaaat gtggatgtgc agatgaatgc
      301 agatgatata ctaaaatgct atgacacctt tgaatggctt gtggaacgat tgcttgatat
      361 catgcagtct gttttttacc gtgtgtcgca gattgatgat gcgctgcgga tttcagttca
      421 tattgtatca gaggtggatc tgcgtggagt catgtcagaa agaccggaat taaaagtgca
      481 gcaggaagat gaaaacgaat ggtttatcag ttgtatcgtc ttcagaaaga gaggtggaag
      541 atgatcgatt ttgtggaatg cggtatggca atgcaggatt ttctgatgat tctttttgaa
      601 atctgcctgg ttttggaact aattctcctg ttggtaagaa tcacctgttt ttccggaaaa
      661 aagaaaatcg taacggcagt ggctgtcttt gctgtttctt ttatgtttat gggcgttctg
      721 atgaatgatc acagatattg tctgggagaa agtttctatc agccaatgct tcaggggtat
      781 ccgttcagcg ttttaatgac ggtggttata ggattattgc tttatctttg ctgggcaatc
      841 cggagcgaga gacgcagata tcatcattct ctgtcttact ggtctgtgaa tgaggctgtg
      901 aatgacgttc cctgtggagt ctgtttttcg gatcctcttg gaaggattgt tttgtgtaat
      961 accaagatgc aggaactgag cagaatcatg acaggttctt atctgcagga ttatgaagca
     1021 ttaaggaaag caatgagtgg ggaacctgaa tcggaaggtc tttgcagatt gagcaaagac
     1081 agcaatgtgt tttattttcc tgatggttct gtctggatgt ttcaggaata tggtctgcag
     1141 gagccggatt gtgcaggata tctgcagaca gtggcagtta atgtatcaga agtttattac
     1201 aatggtgaaa agatcagggc aaacaatgaa aagctggaaa tcctgaatca taagctagag
     1261 gaaatgtacg aaaaaatcgg agataaaatc agagagcagg agacgcttgt catgaaaatg
     1321 caggttcatg acaattttgg acgaagcctt ctgtcaatac gcagaatact ggaaagaaaa
     1381 gaagatccgg ataaaatgga taaacaattg tctgtattga aacatctggt ttatattctt
     1441 accggttctg ctgttgagag tatggaagaa gtatatgagg atacgatcag acatgcagaa
     1501 gagcttggaa tttcagttca gatcagtggg aactttccca tgcatccatc ttacaggctg
     1561 ctgacagaca gggcaatccg tgaatgtgtg acaaactgtg ccagacatgc ccatggcagt
     1621 actgtttggg tgaagataga caaaaatgca gatgaatata ctgttcagat taccaatgat
     1681 ggagaggtgc ctgacaaaaa tgcggaagaa ggcggtggtt tatctgcact gagaaaggca
     1741 gctgaatcag gaaaatgcag gatgcaggtt tctttttccc ctgagttttg tctgatattg
     1801 aaaatgccac tgacagaaag gatgggattc gatggtacgg atactgatag tagaagatca
     1861 gaagatcatg cagaagtatt ttgaatatat tattatgcag gagccggaat tccgacatat
     1921 ccagacggtt tcagatgcac gtgaagctgt aaaatctgtg attattcggc aattgatctt
     1981 gtgatcatgg atgtgcagac gtttcataat catgatgggc tcagtgccgg aaaagaaatc
     2041 agggaaaagt acccatatac aaaggtattg attgttactt ccctgattga tcctaaggtg
     2101 ctggagaggg caaaaagtgg atgcgcagac agtctctggt ataaggatca cggggaagag
     2161 gaaatacgga gtgtgatcca cagaacctta aatggtgagc atgtatttcc ggatatgacg
     2221 ccgaaggtgg agcttaactg gatcacaagc ggtgatatca gtcccagaca gctggagatg
     2281 ctccgcctgt atattcgtgg aatgtcatat tcagagattg cgcgggaaat gaaatgctct
     2341 acctccgggg tgagatggaa ttttcaggaa atgatcgcca gggcaggata cagctgtaaa
     2401 gaagatttga tagcggctgc actggaaagt aaactgatcg taactacgtt aaaatagcaa
     2461 gctgataaac tgcgaagtta ttctgttatg agcagaaagc gaagcggagt gcaaatatcc
     2521 gtttgataag aagggagatg agagtatgaa aaaatattcg gttgctgtat gtgtttgtgc
     2581 ggtactgttt tctgttggta ttcatggcgg aggtatgaga gaagtatctg ccagagcacc
     2641 tggggcagat gagaatctgg caattctgca ggacattatc agggaaaatg aatgtcaggc
     2701 cggaatggca tttctgggat atacctatga aggtgcagag gcagcagaga tacttgaata
     2761 taccagacag ggagaagtgg cagctgcgta tcctttttta caggatggaa cagtggttga
     2821 tgcaggggga tatgaaattt atgccattgt cccgggggat gattgcaggg tcagcgttta
     2881 tccggctcag cctacggaag aaggggaata tctggatgat ctgaacgccc catattatac
     2941 agggaaggag aatgaaatca ttatcctgcg ctgcaatgtg agtgagattc attccaatgt
     3001 gatggtttct gtacagaagc agaatatgag tatacaatac catcctatgg tgtcgttaaa
     3061 ggatggacat cttgcagcag aaacccactg ctatgatttc tccatatatt ataattggga
     3121 tgagtatgat ccggaatata atcccgtgta tgagtcagag tacgaccaag agtgtaatcc
     3181 ggagcatgat tcagcattga atataaagat tgccagagaa cagctttctg agacagatga
     3241 agtcagctat taccttggtc ttgggatgtc t
//
LOCUS       DBMDXG010000253         3255 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6648_length_3255_cov_1.970197, whole genome shotgun sequence.
ACCESSION   DBMDXG010000253 DBMDXG010000000
VERSION     DBMDXG010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6648_length_3255_cov_1.970197"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            23..427
                     /locus_tag="ACZPD3_07365"
     CDS             23..427
                     /locus_tag="ACZPD3_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR1415850.1"
                     /translation="MGKSAGRSEIRKGRPDLQCAENRGQGGFRILMDDFGSGYSSLNM
                     LKEAPVDEIKLDMRFLSAADPYGRAEEILHMIITMGNHMKLSIIAEGVETEQQKVMLQ
                     GFGCNKAQGYFYARPMREAEYTELLRKDKAEN"
     gene            548..817
                     /locus_tag="ACZPD3_07370"
     CDS             548..817
                     /locus_tag="ACZPD3_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR1415851.1"
                     /translation="MYYVPDGTKECGYYECKKCGNRFLSMQTMKRIPCPDCEAEIDYE
                     IGPDESLEDVLDTAELIQKIEGEEEVEKMDTLLSLALTGGDYSWI"
     gene            977..1876
                     /locus_tag="ACZPD3_07375"
     CDS             977..1876
                     /locus_tag="ACZPD3_07375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1415852.1"
                     /translation="MKYDVGIIGSGPAGLSAAIYAKRANLSAVVIEKEYEGTGQIAES
                     GQVDNYPGFPGISGYDLGENFREHAVKLGVSFMEQEVTEIKKEASSDFELVFADGSQV
                     EAKTVIYAAGATPRRANIPGEQEYAGKGVSYCAICDGSFYRGKSVAVLGGGDTALDDA
                     VYLADVAEKVYVIHRRKEFRGAAVTVAKLREKENVIFVLEHQVKEIIGEQKVTGVVLE
                     DAAVIDVNGVFVAYGAVPQTDLLKKFAVLDDSGYVRAGETGETALEGLYVAGDARTKK
                     LRQVVTAVSDGANAATAVAEYLK"
     gene            1989..2858
                     /locus_tag="ACZPD3_07380"
     CDS             1989..2858
                     /locus_tag="ACZPD3_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR1415853.1"
                     /translation="MAQKVDYAALKKGGYMRQKQKGYGSLRLAVVGGNLTAENIKTVA
                     EVAEKYGRGYVHMTSRQGIEIPFIKVEELAEVKEALAKGGVGTGVCGPRVRTVTACQG
                     SEVCPSGCIDTYTLAKELDERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGGMNI
                     EYKEDDCISCGVCVKACRQDALKMVDGKIELDAQKCNHCGRCVKSCPVDAWKGTPGYI
                     VSFGGTFGNNIYKGEELLPLIPDKETLFRVTDAAINFFEKNANPSERFRKTLQRVGEE
                     DFRSQLKDAYEGQ"
     gene            2871..3122
                     /locus_tag="ACZPD3_07385"
     CDS             2871..3122
                     /locus_tag="ACZPD3_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR1415854.1"
                     /translation="MAELDFEVTDEVDITDKVCPLTFVKAKVAIEELEDGEILAVRMN
                     DGEPVQNVPRSMKEEGHKVLKLTDNEDGTYTMYVRKVED"
     gene            3122..>3255
                     /locus_tag="ACZPD3_07390"
     CDS             3122..>3255
                     /locus_tag="ACZPD3_07390"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR1415855.1"
                     /translation="MAVRVSKTDFEEKVLKEKLPVLVDFYSDSCVACKKLAPVLGNAE
                     "
BASE COUNT         1080 a          540 c          898 g          737 t
ORIGIN      
        1 gatatgaata tatcaatgaa tcatgggaaa atcagctggc agatctgaaa tccggaaagg
       61 tagacctgat ctgcaatgcg cagaaaacag aggccaggga ggattccgta ttctgatgga
      121 tgattttgga agtggatatt cttccctgaa tatgctgaag gaagctcctg ttgatgaaat
      181 caaactggat atgcgtttct taagtgcagc agatccttat ggaagagcag aagaaatcct
      241 gcacatgatc attactatgg gaaatcatat gaagctttcc attattgcgg aaggtgtgga
      301 gaccgaacag cagaaggtaa tgcttcaggg gttcggctgt aacaaggctc agggatattt
      361 ctatgccaga cctatgaggg aagcagaata tacagagctg ctcagaaaag acaaagcaga
      421 aaattagatg tacagaaggt tgaataaagg gctgtccggg aatcatcaaa accggcagct
      481 ctttgtcatc agaagaccaa tcatataaaa actaatcata taaacaaaaa aaggagaaaa
      541 ttaaaagatg tattatgtac cagacggcac gaaagagtgc ggatattatg aatgtaaaaa
      601 atgcggcaac cgttttctgt ctatgcagac gatgaagagg attccatgcc cggactgtga
      661 ggctgaaatc gactatgaga tcggtccgga cgagtcactg gaggatgtgc tggatacagc
      721 agaactgatc cagaagattg agggtgaaga agaggtagag aagatggaca cacttctgag
      781 ccttgctctg accggcggag attacagctg gatctagtac agcgaatatt aagtaactgc
      841 tataacctga gtccataacg aatcctaaaa aatttttata atcttattta ttgacagagg
      901 aaattatcgg tgttattatg tttattataa aacatactaa ttcgatagga ataataggtg
      961 taaaggaact gacgatatga aatatgatgt aggtatcatt ggaagcggtc cggcgggttt
     1021 gagtgcggca atctatgcaa agagggctaa tctgtcagcc gtagtgatag aaaaagaata
     1081 tgaaggaacc ggacagatcg cagaaagcgg acaggtagat aactatccgg gatttccggg
     1141 catcagcggt tatgatctgg gtgagaactt ccgggaacat gcagtgaagc ttggggtatc
     1201 cttcatggaa caggaagtaa cagagataaa gaaagaagct tcatcagatt ttgaacttgt
     1261 atttgcagat ggttcgcagg tggaagcgaa aaccgtaatc tatgcggcgg gagccacacc
     1321 gcgaagagcg aatattccgg gagaacagga atatgcagga aagggtgtct cttactgtgc
     1381 aatctgtgac ggatcatttt acagaggtaa atcagtcgca gttcttggtg gtggagatac
     1441 agcacttgat gatgctgttt acctggcgga tgtggcagag aaagtatatg tgatccacag
     1501 gagaaaggaa ttccgaggag ccgctgttac agtggcgaaa ctgagagaga aggaaaacgt
     1561 tatcttcgta ctggagcatc aggtaaaaga gatcatcgga gagcagaaag taaccggagt
     1621 agttctggaa gatgcagctg tgatagatgt gaacggtgta tttgttgctt acggagcagt
     1681 accacagaca gatcttctga aaaaatttgc agtactggat gattcaggtt acgtcagagc
     1741 aggggagaca ggagaaaccg ctctggaagg gctgtatgtg gcaggagatg caagaacaaa
     1801 gaaactgcgt caggtggtaa ctgcggtttc ggatggtgcc aatgcagcaa ccgcagtggc
     1861 agaatatctg aaatagaaga aactgagaga gtattacgat aattcaaccg gttgtaagga
     1921 taccggacag ccgcagatct ggaaaaatat ttgaaaaaac gctctaaaaa cacaaaggag
     1981 agataaaaat ggcacagaaa gtagattatg cagccttaaa aaaaggcgga tatatgagac
     2041 agaaacagaa aggttacggt tccctgagac tggcagtggt cggtggaaac ctgactgcag
     2101 agaatattaa gacagtagca gaagtagcag aaaaatacgg ccgcggatat gtacatatga
     2161 cttcccgcca gggcattgag attcccttta ttaaagtgga agaattggca gaggtaaaag
     2221 aagcactggc aaaaggcggt gtgggaaccg gtgtgtgcgg accacgtgta cgtacagtta
     2281 cagcctgtca gggttcagag gtctgtccaa gcggatgtat cgacacctac acactggcaa
     2341 aagagcttga tgagagatat ttcggacggg aactcccaca taaattcaaa tttggtgtta
     2401 caggatgcca gaataactgc ctgaaagcag aagaaaacga tgttggaatc aaaggcggca
     2461 tgaacatcga atataaggaa gacgactgta tttcctgtgg tgtgtgtgtg aaagcctgca
     2521 gacaggatgc actgaagatg gtggacggca aaatagaact ggatgcgcag aaatgtaatc
     2581 attgtggccg ctgtgtaaag agctgtccgg tggatgcatg gaaaggaact ccgggatata
     2641 tcgtatcttt tggaggaacc ttcggaaata atatctataa aggagaagaa ctgcttcctc
     2701 tgattccgga taaagaaaca ctgtttcgtg tgacagatgc ggcaattaat ttctttgaaa
     2761 aaaatgcaaa tccaagtgaa cgtttccgca aaacattgca gcgtgtggga gaagaagatt
     2821 tccgcagtca gctgaaagat gcgtatgaag gacagtgaaa ggagaacata atggctgaat
     2881 tagattttga agtaaccgat gaggttgata ttaccgataa agtatgcccc ctgacattcg
     2941 tgaaggcaaa agtagcaatc gaagaactgg aggatggaga gattctggct gtccgcatga
     3001 acgatggcga accagtgcag aatgttccgc gcagcatgaa agaagaaggg cataaggtac
     3061 tgaaattaac cgataatgaa gatggtactt acaccatgta tgtaagaaag gtggaagact
     3121 gatggcagta cgggtaagca agacggattt tgaagagaaa gtcctgaagg aaaaacttcc
     3181 ggttctggtg gatttttatt cagattcctg cgtggcgtgt aaaaagctgg caccggttct
     3241 cggaaatgcg gaaga
//
LOCUS       DBMDXG010000254         3241 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6680_length_3241_cov_1.418471, whole genome shotgun sequence.
ACCESSION   DBMDXG010000254 DBMDXG010000000
VERSION     DBMDXG010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_3241_cov_1.418471"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(173..1624)
                     /locus_tag="ACZPD3_07395"
     CDS             complement(173..1624)
                     /locus_tag="ACZPD3_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HIR1415856.1"
                     /translation="MAVLENCEPKRVFHYFEEICKIPHGSGNTKQISDYLVQFAKDHD
                     LKYVQDEMNNVVIYKPGTAGYENAPVVIIQGHMDMVCEKRPDVEHDFTKDGLNLSVEG
                     DYVSANGTTLGGDDGIAVAYGLALLESDTIAHPPLEVFITVDEEIGLLGAVGFDCSVL
                     KGRRFINLDSEAEGSLWISCAGGLSGVSHIPVTRLDAEGEKLTVKISGLMGGHSGAEI
                     DKNRANANSLLGKFLHGLDEKTDFELISVQGGQKDNAITREATAEILVLEENVDAVRE
                     YAASVQGAWREEYAGTDEGITVTVEDEGKQEVRVLHPTSKEKVIFFLVNVPYGVQKMS
                     GTIKGLVETSTNIGILKTSENEVMGSSSIRSSVETARDSLSDKIAYLTEFLGGEYERQ
                     GVYPAWEYRKDSPLRDKMVEVYEEMYGQKPNVVAIHAGLECGLFYKKMEGLDCVSLGP
                     DMKDIHTSEEVLSISSTERVWKYLVKVLEALKE"
     gene            1794..2720
                     /locus_tag="ACZPD3_07400"
     CDS             1794..2720
                     /locus_tag="ACZPD3_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415857.1"
                     /translation="MSKNTILTGGICFLLVFLLLHPAESLSCARAGMTLWLNTLIPTL
                     LPFIILTGMLTHTGHIRKLLLPFESFFHIFPGVSIWGGYVFVLGMLCGYPLGAKLASD
                     LYGLHKISKKEALYLTTFCNNPSPAFIITYLCKSCLKDTVPAGMVFASIFSANLICIL
                     FFRFFVYQNATISDSPGFSPEISSRKNILDFSIMNGFETITRLGGYILLFSILAGCIR
                     YYAPFPLLYQYLLLGFTEITSGLSLISVSGLPYMTRILLSAATTASGGLCILAQTKGV
                     LHHDLSILPYFISKCICTFLTAGILYCLISYL"
     gene            complement(2829..>3241)
                     /locus_tag="ACZPD3_07405"
     CDS             complement(2829..>3241)
                     /locus_tag="ACZPD3_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR1415858.1"
                     /translation="LIADAQARAQELVTQHEIMQKAYAQANDTINAANKQAQEILDSA
                     TQDANSIRLSAITYTDDMMANIGSVLNTTLEDAGVKYKTFIDSLQSCLDVVNHNRQEL
                     APQTAQAAAITGSYHDNDGAKDDDDDLLDDLGEN"
BASE COUNT          834 a          820 c          525 g         1062 t
ORIGIN      
        1 ttttgttatt atagcataga ttttgaaaaa tatctcgcta atatgaatag tttctgtttt
       61 ttttagaaat gcctagttcc gcattctgat acagtatatg caaagcaccg gacatataga
      121 acattcaaaa aagcacccct gccgccaaag cgcaacaggg atgcttccaa catcactctt
      181 taagagcctc cagaactttc accagatact tccagactct ctctgtagaa gaaatactaa
      241 gcacctcttc agacgtatga atatccttca tatcaggtcc cagagacaca cagtcaagtc
      301 cttccatctt cttatagaaa agtccacact caagtcctgc atggattgcc accacattcg
      361 gtttctgtcc atacatttcc tcatatactt ccaccatctt atcacgaagc ggtgaatcct
      421 tgcgatactc ccacgcagga taaacgccct gtctctcata ctctccgcca aggaactccg
      481 tcagataggc aatcttgtca gaaagggaat ctctggctgt ctccacagaa cttctgatac
      541 tggaacttcc cattacctca ttctcagaag tcttcagaat accaatattc gtagaagtct
      601 ctaccagtcc tttgatcgtt ccgctcattt tctgaacacc ataaggtaca tttacaagga
      661 agaaaattac tttttcttta cttgtgggat gaagaaccct tacttcctgt tttccctcat
      721 cttcaacagt tactgtgata ccctcatcag ttcctgcata ctcttctctc catgctccct
      781 gcacagaagc cgcatattct ctgacagcat ctacattttc ttcaagaaca aggatttctg
      841 ctgtagcctc ccttgtaatg gcattatctt tctgtccgcc ctgcacactg atcagttcaa
      901 aatctgtttt ctcatccaga ccatggagaa attttccgag aagagaattt gcgtttgcac
      961 gattcttgtc aatctcagct ccggaatgac cgcccatcag tccgctgatc ttaactgtca
     1021 gcttctctcc ctctgcatcc agtcttgtca ctggaatatg gctgactccg gaaagtccgc
     1081 ctgcacagct gatccagagg cttccttctg cttcagaatc cagattgata aatcttcttc
     1141 cttttaatac actgcagtca aatccaactg cacccagaag accgatttcc tcatctacag
     1201 taataaacac ttcaagcggc ggatgagcaa ttgtatcact ttccagaagt gcaagaccat
     1261 aagcaacagc aattccgtca tcaccaccaa gggttgttcc gtttgcagat acatagtcac
     1321 cttctacaga aagatttaat ccgtcttttg taaaatcatg ctctacatcc ggacgtttct
     1381 cacataccat atccatatgt ccctgaatga tcactacagg agcattctcg tatcccgctg
     1441 tgcccggttt ataaataacc acattattca tctcatcctg aacatatttc agatcatgat
     1501 cctttgcaaa ctgaaccaga tagtcgctga tctgctttgt atttcctgag ccatgaggaa
     1561 ttttgcatat ctcctcaaaa taatggaaca ctcttttggg ttcacaattc tctaaaactg
     1621 ccataattat attacctcca aaaatcacac atttgtctta taatatacca ttgttttccc
     1681 gatatacgac aaattttcca gatatatttt acccaaatac tatagcattt attttcattc
     1741 tgcgccatat aaattttata aagaatggca aacaattgca aagaggttgt tgtatgtcca
     1801 aaaacaccat actcaccggt ggtatttgtt ttcttcttgt ctttcttctg ctacatcctg
     1861 ctgaaagtct gtcctgtgcc agagccggaa tgactctgtg gctgaataca ttaatcccaa
     1921 cgcttctccc atttattatt ctcacaggaa tgcttacaca taccggtcat atccggaaac
     1981 ttctcctgcc atttgaatca ttctttcata tttttccggg tgtcagcatc tggggtggtt
     2041 atgttttcgt tctcggaatg ctgtgtggtt atcctctggg agccaaactt gcctctgatc
     2101 tgtatggatt acataaaatc agcaaaaaag aagctcttta tctcactacc ttctgtaaca
     2161 atccaagccc tgcctttatc attacctatc tctgtaagtc ctgtcttaag gatacagtac
     2221 ctgccggcat ggtctttgcg agtatttttt cagcaaatct gatatgtata ctgttctttc
     2281 gtttttttgt gtaccaaaat gccaccatct cagattcccc aggattttca cccgagattt
     2341 cttctcgaaa aaatatactt gatttttcta taatgaacgg ttttgaaacc attacacgtc
     2401 tcggaggata tattctattg ttctccattc tggcaggatg tatcagatat tatgcgcctt
     2461 ttccacttct ctatcaatac ctgctgctcg gatttacaga gatcacttcc ggactttctc
     2521 ttatttcagt ttccggactg ccatacatga ctcgtatact tttgtccgct gcaaccaccg
     2581 catccggcgg tctgtgcatc cttgctcaga caaaaggtgt tctccaccat gacctcagca
     2641 ttctgcctta ttttatttcc aagtgtatct gtacattcct tacagcaggt attctttact
     2701 gtctgatttc ctatctgtga aaataaattc tttccgggaa ctgtcatcat ttcattatac
     2761 aaaatcaggg caggaaattt tcctgcccct tgttttctca tcttctatat ttaataactg
     2821 atttattttt agttctctcc cagatcatct aacagatcgt catcatcatc tttcgctccg
     2881 tcattatcat gataagagcc tgtaattgct gcagcctgtg ctgtctgagg tgcaagttcc
     2941 tgacgattgt ggtttacaac atcaagacag ctctgcaggg aatcaataaa tgttttgtat
     3001 ttcacacctg catcctctaa tgttgtatta agcacactgc caatattggc catcatatca
     3061 tctgtatagg tgatcgcgct gagacgaatg ctgtttgcat cctgtgttgc agaatccaga
     3121 atctcctgtg cctgtttgtt agctgcattg atcgtatcat ttgcctgtgc atatgctttc
     3181 tgcataatct catgctgtgt taccagttcc tgtgctcttg cctgcgcatc tgcgatcagt
     3241 g
//
LOCUS       DBMDXG010000255         3240 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6684_length_3240_cov_1.694807, whole genome shotgun sequence.
ACCESSION   DBMDXG010000255 DBMDXG010000000
VERSION     DBMDXG010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6684_length_3240_cov_1.694807"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="ACZPD3_07410"
     CDS             <1..930
                     /locus_tag="ACZPD3_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR1415859.1"
                     /translation="ASVYSASKLATEEFPNFDVGQRSAASIARRVQDPLAELVKIDPK
                     SIGVGQYQHDMNQKKLDEALSGVVEDSVNKVGVDLNTASASLLEYISGISKAIAKNIV
                     AYREENGQFTDRKELLKVAKLGPKAFEQCAGFMRISGGKNPLDATSVHPESYEAASAL
                     LSKLGYKPNDVVAGNLLGLSLQVKDYKKMAAELGIGEITLRDIVKELEKPARDPRDDM
                     PKPILRSDVLEMKDLKEGMVLKGTVRNVIDFGAFVDIGVHQDGLVHISEMTERFIKHP
                     LEAVSVGDIVDVRVIGVDMKKKRISLSMKGLNK"
     gene            complement(1018..1911)
                     /locus_tag="ACZPD3_07415"
     CDS             complement(1018..1911)
                     /locus_tag="ACZPD3_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1415860.1"
                     /translation="MILDYYRIFYYVAQYKSFSKAADVMGNNQPNITRCMNILENELG
                     CKLFIRSNRGVQLTIEGERLFEHVSIAIEQLVSGENELLKDKGLESGLVNIGASEIAL
                     RLFLLNELEVFHHRYPHVKLRISNHSTPQAVQALENGLVDFAVVTTPVALKKPLQRIP
                     LYSFREILIGGEEYAETASNKHCLQDLQDIPLIGIAPGSSTRELYTQYFMRHNLPFSP
                     DMEVATTDQIFPMVQHNLGIGFFPEELAAEHIAQGKIVQIPIEEPLPERKICLIRNVK
                     RPQSIAARSLEEHLTSHRETE"
     gene            2075..2308
                     /locus_tag="ACZPD3_07420"
     CDS             2075..2308
                     /locus_tag="ACZPD3_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1415861.1"
                     /translation="MTTANKTIKINDVEAAKKLVCAAVRCPFDIDIVSKGKIFIDAKS
                     ILGVLSLDVEEPLELKYDGYDEDFESVIAGLAC"
     gene            2337..2630
                     /locus_tag="ACZPD3_07425"
     CDS             2337..2630
                     /locus_tag="ACZPD3_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIR1415862.1"
                     /translation="MTSTNLLYIYVIIINVVTFFIYGLDKSRAKAGQWRIPEAQLIFL
                     AVIGGSVGALAGMKVFHHKTRKPKFKTGVPAILIIQLIIYFLFSGNEGIILRL"
     gene            2938..>3240
                     /locus_tag="ACZPD3_07430"
     CDS             2938..>3240
                     /locus_tag="ACZPD3_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR1415863.1"
                     /translation="MEQEIQEAKSLYYSYKQYFSDYLPALTKLGLKVVMALLVFVGGR
                     KVIQWFVSFIKKSMERASVDKGVIQFTGSLLRIVLYILLVFSIATHFGVRESSIAAL"
BASE COUNT          990 a          559 c          776 g          915 t
ORIGIN      
        1 gcatctgtgt attctgcaag caaactggcg acagaggaat tcccgaactt tgatgtagga
       61 cagagaagtg cggcttccat tgcaagacgt gtacaagatc cgcttgcaga actggttaag
      121 atcgatccga aatctatcgg tgtgggtcag tatcagcatg atatgaacca gaagaaactg
      181 gatgaagcat taagcggcgt tgtagaagac agtgtaaata aagttggtgt agatttaaat
      241 acagcatctg catctttact tgagtatatt tccggtatca gcaaagcaat tgccaaaaat
      301 atcgtagctt acagagaaga gaacggtcag ttcacagaca gaaaagaact tctcaaagta
      361 gcgaaattag gaccgaaggc atttgaacag tgtgcaggtt tcatgcgtat ttcaggtgga
      421 aagaatcctc tggatgcaac aagtgtacat cctgagagct atgaagcagc atctgcactt
      481 ctttcaaaac ttggatataa gccaaatgat gttgtggcag gaaatctcct tggactttcc
      541 ctgcaggtaa aagattataa gaagatggca gcagaactgg gaatcggtga gatcacactt
      601 cgtgatattg taaaggaact ggagaaaccg gcccgtgatc ctcgtgatga catgccgaaa
      661 ccgatccttc gttcagacgt tcttgaaatg aaagatctga aagaaggaat ggtgctgaag
      721 ggaactgtcc gtaatgttat tgatttcggt gcatttgtag atatcggtgt acatcaggac
      781 ggtctggtgc acatttccga gatgacagag agatttatca aacatcctct ggaagcggtg
      841 agtgtagggg atatcgtaga tgtaagagta atcggagttg atatgaagaa aaaacgtatc
      901 agcttatcca tgaaaggatt aaataaataa catcaataaa acagggatta ccactgcaga
      961 ccggatatct gcaggagtga tccctgtttt tgggggtact gctgtctgca gtaacagtta
     1021 ttctgtttcc cggtgtgatg tgaggtgttc ttcaaggctg cgtgctgcaa tgctctgtgg
     1081 cctttttaca ttgcggatca gacagatctt tcgttcagga agaggctctt caatgggaat
     1141 ctggacgatt ttcccctgag caatatgttc agcagcaagc tcttcaggga agaatccgat
     1201 tcccaggttg tgctgcacca tagggaagat ctggtcagta gttgccactt ccatatcagg
     1261 tgagaaggga aggttatggc gcataaaata ctgtgtatat aattctcttg tactgcttcc
     1321 gggagcaatc ccgatcagag ggatatcctg cagatcctga agacagtgtt tgttggaagc
     1381 tgtttccgca tattcctctc cacctataag gatctcgcgg aaagaataga ggggaattct
     1441 ctgaagtggt tttttcagag cgacaggtgt ggtaactact gcaaagtcta ccagaccgtt
     1501 ttccagtgcc tgaactgcct gtggtgtgga atgattagaa atcctcagct tcacatgtgg
     1561 ataacggtga tgaaagactt ccagttcgtt cagcaggaat aaacgcagtg cgatttcact
     1621 ggcaccaata tttacaagtc cgctttccag acctttatct ttgagaagtt cattttctcc
     1681 tgatacaagc tgttctattg caattgatac atgttcaaaa agtctttctc cttcaatggt
     1741 cagctgtact ccgcgattgg aacggataaa aagtttacat cccagctcat tctccagaat
     1801 attcatacac cgggtaatgt taggctgatt gtttcccata acgtcggctg ctttggaaaa
     1861 actcttatac tgggcaacat agtaaaaaat acgataataa tcaagaatca tgatacagtc
     1921 ggctccttat gctatataaa aatagaatat ctgatataga aaaataacgt ttgtatatca
     1981 atgtgtgaat cagtataata cgactgggtc aaaaaatcaa tataaaagtc ctgacaatag
     2041 gacggagacc aaaagggaag ggagattacc gaatatgaca acagcaaata agacaattaa
     2101 gattaatgac gtagaggcag caaagaaact ggtctgtgcg gcagtcagat gtccatttga
     2161 tattgacatt gtatctaaag gaaaaatttt tattgatgcc aaatctattc tcggagtatt
     2221 aagccttgat gtggaagaac ctctggaact taaatatgat ggttatgatg aggattttga
     2281 atcagtgata gcaggtctgg catgttaaga ttaaagaaag gaattcgcag gtgcagatga
     2341 catctacaaa tcttctatat atttatgtga ttatcattaa tgtagtcaca ttttttatct
     2401 atggcttgga taaaagcagg gcaaaagccg gacagtggcg cataccggaa gcgcagctga
     2461 tatttctggc ggtgatcgga gggtcggtgg gagctctggc cggtatgaag gtatttcatc
     2521 ataaaacaag aaagccgaaa tttaagacag gagttccggc gatactgatt attcaactaa
     2581 ttatttattt tttgttttcg ggaaatgagg ggattattct gcgcttatag caatccttat
     2641 aaataaggca tattgatttt agctcatcag agcgtgaaat cctgtgtcag taaagcggat
     2701 atattgtccg ctttcttttt ttgcccgaat aaaattgatt gttacagaaa atgaggtatg
     2761 ttatgctact cttatatgag aattgcggga tctgcaaagt agcggagtaa ttcgtagata
     2821 tcaagtcagg ggaaagacat cttttaacac tgatattata tgaaaagctg cctgttaagt
     2881 gaaagaaggg cagaaaaata atacatgaca aaaatataag agagcaggtg aaaaatattg
     2941 gaacaggaaa ttcaggaggc aaaaagttta tattacagct ataagcagta tttttcagac
     3001 tatcttccgg ctttgacgaa actggggctg aaagtagtaa tggcattgct ggtatttgtg
     3061 ggcgggcgca aagtgatcca gtggtttgtt tctttcataa agaagtccat ggagcgtgcg
     3121 tctgtagaca aaggtgtgat ccagtttaca gggtcgctgt tgagaattgt actttacatt
     3181 ttgctggtat ttagcattgc cactcatttt ggtgtaaggg aatcctctat tgcagcactg
//
LOCUS       DBMDXG010000256         3233 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6702_length_3233_cov_1.666667, whole genome shotgun sequence.
ACCESSION   DBMDXG010000256 DBMDXG010000000
VERSION     DBMDXG010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6702_length_3233_cov_1.666667"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(73..143)
                     /locus_tag="ACZPD3_07435"
     tRNA            complement(73..143)
                     /locus_tag="ACZPD3_07435"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(109..111),aa:Trp,seq:cca)
     gene            complement(254..341)
                     /locus_tag="ACZPD3_07440"
     tRNA            complement(254..341)
                     /locus_tag="ACZPD3_07440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(305..307),aa:Ser,seq:cga)
     gene            544..1470
                     /gene="epsC"
                     /locus_tag="ACZPD3_07445"
     CDS             544..1470
                     /gene="epsC"
                     /locus_tag="ACZPD3_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIR1415864.1"
                     /translation="MTDKKKTILEAVEKLTDTYRKEELFLGKDRERLPNKKEIINFIK
                     DMRSIIFPGYFSVDSSASVFPEHYVAYRLNDLYDCLQEQIEIAFLYQGEEEQKAKEHA
                     EQITERFFANVPEIQRMLLTDLQAGFDGDPAAKSKEEIIFSYPGFYAIYVYRLAHVLY
                     LENVPFIPRIMSEYAHGYTGIDINPGATIGEYFFIDHGTGVVIGETTEIGKNVKLYQG
                     VTLGALSTRQGQLLANVKRHPTIRDNVTIYSNSSVLGGETVIGENTIIGGNTFITASI
                     PANTKVSAKSPELVIKKPRSSVEATNVWDWEN"
     gene            1808..1878
                     /locus_tag="ACZPD3_07450"
     tRNA            1808..1878
                     /locus_tag="ACZPD3_07450"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1840..1842,aa:Gly,seq:tcc)
     gene            1882..1954
                     /locus_tag="ACZPD3_07455"
     tRNA            1882..1954
                     /locus_tag="ACZPD3_07455"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1915..1917,aa:Gly,seq:gcc)
     gene            1972..2042
                     /locus_tag="ACZPD3_07460"
     tRNA            1972..2042
                     /locus_tag="ACZPD3_07460"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Gly,seq:tcc)
     gene            2046..2118
                     /locus_tag="ACZPD3_07465"
     tRNA            2046..2118
                     /locus_tag="ACZPD3_07465"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2079..2081,aa:Gly,seq:gcc)
     gene            complement(2288..2497)
                     /locus_tag="ACZPD3_07470"
     CDS             complement(2288..2497)
                     /locus_tag="ACZPD3_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HIR1415865.1"
                     /translation="MGSKCLRYTALTISIIGAVNWGLIGFFNLNLVALLFGSMSWISR
                     IIYGLVGICGLCLLTFYGDSDTESE"
     gene            complement(2694..>3233)
                     /locus_tag="ACZPD3_07475"
     CDS             complement(2694..>3233)
                     /locus_tag="ACZPD3_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HIR1415866.1"
                     /translation="VIKTDSGWKIVSLDDETVKIMSANFKSVEEEINNSLNNMDNEDS
                     SGSSSNAPEASADDTLNLTTEKFTIKYTKHTITKDFAGNPCIMVYYDYTNNSSSASSA
                     MVDVSLKAYQHGESCEAAIPENNDDAIDHFTAEIQPGQTVNVCQAFTLTDESDVTVQA
                     QEAFSFDEDATARQILKVK"
BASE COUNT          919 a          638 c          733 g          943 t
ORIGIN      
        1 taggaataaa tatcttctgc ttttaagtta atgacattga gtaaatacaa ggctttcgag
       61 cgtttttaag agcaggggat gagagaatcg aactcccacc aaaggttttg gagaccccta
      121 tcataccatt tgaccaatcc cctatcaaaa ccaacttgta aaatttcttt tactgttgca
      181 attatacata taaaagcaac gattgtcaag cttattgtgc atttttatta taaagaattt
      241 tacaaagaaa gagcggagat ggagggattt gaaccctcgc accggttgcc cggcctgccg
      301 catttcgagt gcggtccctt cagcctcttg ggtacatctc catattaaga ttagaaacag
      361 ttctgaagtg taaccggaac tgttttttat tatataagga atctacggaa aggtcaattc
      421 tttttttatt gacgaattgg ttttgctgta ataagataat aacaaaatgc ggttgtgtac
      481 cctctggatt gctattgtcc ggggataata agcagctgcg aggaaagcag ataggtgacg
      541 attatgacag ataagaagaa aacaattctg gaggctgtgg agaagctgac agatacttac
      601 cgtaaagaag aactctttct gggaaaggac agagaacgtc ttccaaataa gaaagagatt
      661 attaacttca ttaaagatat gaggtctatt atttttcccg gatatttcag tgtggattcc
      721 agtgccagcg tgtttccgga gcattatgtg gcatatcgcc tgaatgatct gtatgactgc
      781 ttgcaggagc agattgagat tgcattttta tatcagggtg aagaagaaca gaaggccaaa
      841 gaacatgcag agcagatcac agagcgattt tttgcaaatg ttccggaaat tcagcgtatg
      901 ctgctgactg acctgcaggc aggttttgac ggtgacccgg ctgccaagag taaagaggag
      961 atcattttct cttatcctgg attttatgca atttatgtat acagacttgc acatgttctg
     1021 tatctggaaa atgtgccgtt tattccaaga attatgtctg aatatgcaca tggttataca
     1081 ggtatcgata tcaatccggg tgctacgatt ggagaatatt tcttcattga ccatggaacc
     1141 ggtgttgtaa tcggtgagac tacggagatt ggaaagaatg tgaagctgta tcagggcgtg
     1201 actcttggcg cactttccac ccgtcaggga cagctgcttg ccaatgtgaa gagacatccg
     1261 acgatcagag ataatgtgac gatttattct aactcttctg tgctgggcgg tgagactgtg
     1321 ataggagaga atacgatcat cggaggtaat acctttatta cagcatctat tccggcaaac
     1381 accaaggtca gtgcgaagag tcctgaactt gtgattaaga aaccaagaag ttcagtggaa
     1441 gctacgaatg tatgggattg ggagaactga tctctggtta acactgtaaa attttgtttt
     1501 cctgacaggt tccatcatat acagtaagtc caggctttca tcgtggaaag taataagtag
     1561 caggtggaaa tttgcttata tcaggaggag tgaaagctcc ttctgataaa ctgcatagca
     1621 attattcgat atagagctga gcagcgaaac ggatgatttt atctgcgtga caaattgttt
     1681 gccagatgat gatttgtata tgttgttaaa aaaatagaaa aaatttaaat ttagtgttga
     1741 caaatacagt ttcatcatat atactagtct tcgttgatgc gacagagata cacagacaac
     1801 aagctgtgcg ggtgtagttc aatggtagaa caccagcctt ccaagctgga tacgtgggtt
     1861 cgattcccat cacccgctta tgcgatagtg gcgtagttgg taacgcgcga ccttgccaag
     1921 gtcgagaccg cgggttcgag ccccgtctat cgctctttca actgaatatc tgcgggtgta
     1981 gttcaatggt agaacaccag ccttccaagc tggatacgtg ggttcgattc ccatcacccg
     2041 cttgtgcgat agtggcgtag ttggtaacgc gcgaccttgc caaggtcgag accgcgggtt
     2101 cgagccccgt ctatcgctct tgggacttcg gattttccga agtccttttt tatattatag
     2161 gaaattactc cgtaatgttc ctataacata aaaaagccct tcgggcagga tcgcactgcg
     2221 gcgtagagga attatgtcgc tgaagcgacc cgccgcaggc tgagaatcct gcttgcagga
     2281 ttctttctta ttcagattca gtgtctgaat cgccgtagaa ggtcagaaga cagagtccac
     2341 agattcctac cagaccatat atgattctgg aaatccagct catgcttccg aaaaggagag
     2401 caaccagatt cagattgaaa aaaccgatca gtccccagtt aacagctccg atgatggaaa
     2461 tcgtcagtgc cgtatatctt aaacatttac ttcccataag tcaccatcct ttcgtctccc
     2521 tgtggctgca gacagctctg acaggaatca taaagctatt acatttcata ttgttccatg
     2581 gaagacaaaa aatatgcaca gcaggttcaa attaatttta aatcgctgtg catattttaa
     2641 cagaatcaaa taagttctct gcctcaattg aggaactctg tctgacaatc ggattatttt
     2701 actttaagta tctgtctggc agttgcatcc tcatcaaagg aaaatgcctc ctgtgcctga
     2761 accgttacat cactctcatc tgtcagtgta aatgcctggc atacattaac ggtctgtccg
     2821 ggctgaatct cagctgtaaa atgatcgatc gcatcgtcat tattctcggg aatcgctgcc
     2881 tcgcaggatt ctccatgctg gtaagctttt aggctgacat caaccattgc actggatgca
     2941 gaggaagaat tattggtgta atcgtaataa accataatac atggatttcc ggcaaagtct
     3001 ttggtgatcg tatgtttggt atatttgatc gtaaattttt ctgttgtgag attaagcgta
     3061 tcatctgcag atgcctccgg tgcattggaa gaagaacctg aggaatcttc gttatccata
     3121 ttgttgaggg agttgtttat ttcctcttca acactcttga agtttgctga catgattttt
     3181 actgtctcat catccagaga aacaattttc catccggaat ctgttttgat cac
//
LOCUS       DBMDXG010000257         3230 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6710_length_3230_cov_2.450943, whole genome shotgun sequence.
ACCESSION   DBMDXG010000257 DBMDXG010000000
VERSION     DBMDXG010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6710_length_3230_cov_2.450943"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="ACZPD3_07480"
     CDS             <1..2154
                     /locus_tag="ACZPD3_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR1415867.1"
                     /translation="DFFSDEKEMPSVQEGDTLVANAGQGITAGTSTYSSKSSFGRRKA
                     LSQLQGMGINSGSYSWNWASPEYTSYYTDEAGNLHIVAWKDQTLYDAVCNSDLNVTNV
                     TTVKLPLPLWGGFYAAPDGNFYIAAGQKNLNEDNSITAVRILKYSRAWKLLGATDIGG
                     GYTNMFEGIYIPFDAASLRMTQIGSTLIVHTGREMYGMEGIHHQSNITFVINTQDMTL
                     INSDMPYCSHSFNQFVVNDGSHVYFLDHGDAYYRGLILSSFSAYSGGYIAQDRAVNIF
                     PFMGATGDNYTGCEVTGFSLAGNNLITVGKSVPHGLAVNGQTGYENLNKNIFMIITDK
                     NSMTSRFIWLTQYSPSGAEITLTEPKLIPVGNNQYAVLFSEETSNQSVLHYLLMDMSG
                     NVKLSKLYKNVTIQTDSQPILWGRNIVWVSGNYDNGNYDSSRTYLYEIPVVTTPLNGI
                     ALNQTNLTIDEGNTQKLTPSFTPSNSDDVKDVVWTSSNPGVASVSEDGTIQGNGYGQA
                     VITASAGDFQAQCQVTVKVSENNSPLTKPVLKLSQKAADQIHLTWKKIPGAKGYQIYC
                     KTDSRSSYKRIKTLKTGSLSFDAAVVPGVTYSFKVRAYGTNASGKNKYSKFSAVKSRK
                     AAVPAPSKVSCKMSNGGTEVSWTKVAGASGYVIYRNGSAAKTVKSSVSTWKDTKAYDS
                     QTGMYWVYNYYVKAFKTVNGKRIYSKPTKTVNLYS"
     gene            complement(2258..3121)
                     /locus_tag="ACZPD3_07485"
     CDS             complement(2258..3121)
                     /locus_tag="ACZPD3_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR1415868.1"
                     /translation="MKIAVTYDNGEIFQHFGKTESFKVYEVEDNKVVSSEVIGSNGTG
                     HGALAGLLAEQGVNVLICGGIGGGAQTALTEAGIELCAGAQGNTDQAVESYLKGELVS
                     SGANCDHHHHEEGHSCGSHEEGHSCGDSCGGGCGGCGGSQPQLTGRNVGKTCRTHYRG
                     TFNDGTQFDSSYDRGEPLEFICGAGQMIRGFDAAVADMEVGQIIDVHLMPEEAYGMAD
                     PNAIFTMEIAQLPGSEDLTVGQQVYLSNQFGQPFPVKVTAKDEKNITFDANHEMAGKE
                     LNFKIELVEVK"
BASE COUNT          924 a          728 c          680 g          898 t
ORIGIN      
        1 gatttctttt cggatgaaaa ggaaatgcct tctgttcagg agggggacac gttggttgca
       61 aatgcagggc agggaataac agctggtact tccacatatt cctcaaagtc ttcctttggc
      121 cggagaaagg ctctttccca gcttcagggt atgggcatca acagcggatc ttacagctgg
      181 aactgggcaa gtcctgaata tacttcttat tatacagatg aggcaggaaa tcttcatatt
      241 gttgcctgga aagatcagac tttatatgat gctgtctgca attccgatct taatgttacc
      301 aatgtaacaa cagtaaagct tccgcttcca ttgtggggag gattctatgc tgcacccgat
      361 gggaattttt atattgcagc aggacagaaa aatctgaacg aagacaacag cataacggct
      421 gtccggattc tcaaatatag ccgtgcctgg aaactgcttg gtgcaacgga cattggcggc
      481 ggatacacaa atatgtttga aggaatctac attccttttg atgctgcttc cctgcgcatg
      541 acccagatcg gttctaccct gatcgtacac acaggacggg aaatgtatgg gatggaaggg
      601 atacatcatc agagtaatat cacttttgtc attaacacgc aggatatgac gcttattaat
      661 tcagatatgc cttattgtag ccattccttt aatcagtttg ttgtaaatga cggctctcat
      721 gtgtattttc tggatcatgg ggatgcttat tacagaggtc tgatcctgtc ctccttctcc
      781 gcatattccg gcggatatat cgctcaggat cgtgcggtaa atatctttcc ttttatggga
      841 gctacaggtg ataattatac cggatgcgag gtaaccggat tttctctggc aggaaataat
      901 ctgatcacag taggcaagtc tgttccccat ggtttggctg tgaatggaca gaccggatat
      961 gaaaatctta acaaaaatat ctttatgata attacagata agaacagtat gacttccaga
     1021 tttatctggc tcactcagta ttcaccatca ggagcagaaa taactctgac agaaccaaag
     1081 ctcatccctg taggaaataa tcagtacgca gtccttttct cagaggaaac ctccaaccag
     1141 agcgttctgc attatctcct tatggatatg tccggaaacg taaaattatc caagttatac
     1201 aagaatgtaa cgatccagac ggattcacag ccgatcttat ggggacgtaa tattgtatgg
     1261 gtatctggaa attatgataa cggcaactat gacagttccc gcacatacct gtatgaaatc
     1321 cccgtagtga caactccact taatggaatt gctctaaacc agacaaatct gacaattgac
     1381 gaaggcaata cacaaaaact cacgccttcc tttacaccat ccaactctga tgatgtaaaa
     1441 gatgtggtct ggacaagcag taatcctggg gttgcgtctg tctcagagga tggaaccatt
     1501 caggggaacg gttatggtca ggcagtaatc actgcttctg ccggagattt tcaggcccag
     1561 tgtcaggtga cggtaaaggt atctgagaac aactcaccac tgacaaaacc tgtgctgaaa
     1621 ctttcccaga aagctgcgga tcaaatacat ttaacctgga aaaagattcc gggtgccaaa
     1681 ggataccaga tttactgcaa gacagattcc cggtcttctt ataagcggat aaaaactctg
     1741 aaaacaggat ccctcagttt tgatgcagct gtagtaccgg gagtaaccta ttcatttaaa
     1801 gtccgtgctt atggaaccaa cgcaagtgga aaaaacaaat attctaaatt ttcagcggtg
     1861 aaatcaagaa aagctgctgt cccggctcca tctaaggttt catgtaaaat gagtaacggt
     1921 ggtacagagg tttcctggac aaaagtagcc ggtgcttccg gatatgtaat ttaccgcaat
     1981 ggttccgctg ccaaaacagt gaagtcctct gtctcaacct ggaaagacac aaaggcttat
     2041 gattcccaga caggcatgta ctgggtttat aactattatg taaaagcttt taagacagta
     2101 aacggcaagc gaatatacag taagcccaca aaaacagtaa atctttactc gtaaattata
     2161 aaactacaaa aagcactatc actttggtaa ataaagtata aaacagggca gtctcatcgg
     2221 caaaattttt gccgggaggc tgccctgagt atttgtctta tttcacttct actaattcaa
     2281 tcttgaagtt aagttctttt ccagccattt cgtggtttgc atcaaatgta atgtttttct
     2341 catccttagc tgtcacttta acagggaatg gctgaccgaa ctggtttgac aggtatacct
     2401 gctggccaac tgtcaggtct tcggaacctg ggagctgggc gatttccata gtgaagatag
     2461 cgtttggatc tgccataccg tatgcttctt caggcataag gtgaacatca atgatctgac
     2521 caacttccat atcagctaca gcagcatcaa atcctctgat catctggcct gcaccgcaga
     2581 tgaattccag cggttcgcca cggtcgtaag aagaatcaaa ctgtgttccg tcattgaaag
     2641 ttcctctgta atgtgtgcgg caggtttttc ctacattgcg tcctgtaagc tgtggctgtg
     2701 atccgccaca tccaccgcat ccgccaccgc agctgtctcc acaggagtgt ccctcctcat
     2761 ggctgccaca ggaatgacct tcttcatgat ggtgatggtc gcagttagct ccggaagaca
     2821 ctaattcacc ttttaaatag ctttcaactg cctgatcagt atttccctgg gctccggcgc
     2881 ataattcgat tcctgcctct gtgagagctg tctgagcacc accgccgatg ccaccgcaga
     2941 tcagaacatt tacaccctgc tcagcaagca gacctgcaag tgcaccatgt cctgtaccgt
     3001 tagaaccgat cacttcactg gaaactactt tgttgtcttc cacttcataa actttaaaag
     3061 actctgtttt tccaaaatgc tgaaaaatct ctccattatc atatgttact gcaattttca
     3121 tttgaaaaat cctccaaatt attttttata cccattcagt ccagtataaa ctgtctgcga
     3181 cacaaaagca aggctatttg atcaggtaat tctcaattcc atttgcaatt
//
LOCUS       DBMDXG010000258         3208 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6760_length_3208_cov_1.443836, whole genome shotgun sequence.
ACCESSION   DBMDXG010000258 DBMDXG010000000
VERSION     DBMDXG010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6760_length_3208_cov_1.443836"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /gene="rnc"
                     /locus_tag="ACZPD3_07490"
     CDS             <1..647
                     /gene="rnc"
                     /locus_tag="ACZPD3_07490"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR1415869.1"
                     /translation="YHFKNKHYLTQALTHSSYANEKKLGKLGSNERLEFLGDAVLELI
                     SSDYLYARFTQIPEGELTKKRASLVCEPSLAYCAREFGLPQFLLLGKGEDMTGGRNRD
                     SIVSDATEALLGAIYLDGGFANAKEFVLNFILNDIEHKQLFYDSKTILQEIVQENGTQ
                     PVEYILTKEEGPDHNKNFTVEARVNGKVMGQGSGHTKKAAEQAAAYQAIRVLRK"
     gene            659..>3208
                     /gene="smc"
                     /locus_tag="ACZPD3_07495"
     CDS             659..>3208
                     /gene="smc"
                     /locus_tag="ACZPD3_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR1415870.1"
                     /translation="MYLKNIEVQGFKSFAQKINFEFHNGITGIVGPNGSGKSNVGDAV
                     RWVLGEQSARSLRGGNMQDVIFSGTETRKPLGYASVAITLDNSDHKLPVDFNEVTVTR
                     RLYRSGESEYKINGSACRLKDINEMFYDTGIGKEGYSIIGQGQIDRILSGKPEERREL
                     FDEAAGIVKFKRRKNTTIKKLEEERQNLVRVTDILSELTRQLEPLEKQSETARVYLSK
                     RETLKELDVNMFLMEYAAAAKELKELEEKNIIAQNQLKDTQSSYDRTKVEYERLEQEL
                     EDLNSKMDALRISGQEQAIRKQQLEGQINVLNEQILAGAQNEEHYKGRIQTIEAELSV
                     RTDSKKKLEEEKTDIYAQLKAVRKKLSEEEEKLRTAQENMEACTLEVENGKNEIIEIL
                     NSRANVKGKAQRFDAMMEQAEIRKAEISQRILRLKSEEEEQQTILTTARKQYDEITSQ
                     IENANEECEHLNLSVMKIQEKLKEQNTKLEVEQTAYHREASRLDSLRNIAERYDGYGN
                     SIRRVMEQKERVPGIQGVVADLIQVNKDYEIAIETALGGSIQNIVTDNEQTAKTMIEF
                     LKKNRYGRATFLPMSSISPRGEFTPKEALKEPGVVGIASELVSVASQYQQITKFLLGR
                     VLVVDNIDHAIAIGKKYRHSLRMVTTEGESLNPGGSMTGGAFRNNSNLLGRRREIEEL
                     ETKVNQLKQNLTEMQNAVEENKNQRNRLRDAIAGFQEKLRQKYIEQNTARMNIKLQEK
                     KAEEIRSGYAQINRDQAEIKRQVMEIRQDHDRIARELENSKQDEKELESFIEAKQSEL
                     DEWKEEEKKITRELEEIRLQSSALEQKEKFDQENLSRLKAEITAFMTEKEDI"
BASE COUNT         1110 a          561 c          860 g          677 t
ORIGIN      
        1 gatatcattt taaaaataaa cattatctta cacaggcact gactcacagt tcctatgcaa
       61 atgagaagaa gttggggaag ctgggcagca atgagagact ggagtttctg ggagatgctg
      121 tgctggagct gatcagcagt gattatctgt atgcaagatt tacccagata ccggagggag
      181 agctgacaaa gaagagagcc agcctggtgt gtgagccaag ccttgcctat tgtgcaagag
      241 aattcggact gccgcagttt ctgttgcttg gaaagggaga ggatatgacc ggcggaagaa
      301 acagggattc tatcgtctct gatgcaacag aggctttatt gggagctatc tatctggatg
      361 gtggttttgc taatgcaaag gagtttgtgc tgaattttat cctgaatgat attgaacata
      421 aacagctctt ttatgatagc aagaccatcc tgcaggaaat cgtgcaggaa aacggaacac
      481 agccggtgga atatattctg acaaaagaag aaggcccgga tcataacaaa aactttactg
      541 tggaggcaag agtaaacgga aaagtgatgg gacagggaag cggtcataca aagaaagcag
      601 cagaacaggc cgcagcatac caggcgattc gtgtactcag aaaataaaca ggagaattat
      661 gtatttaaag aatatagagg tacagggatt taagtctttc gcccagaaga tcaattttga
      721 atttcataac gggatcaccg gtattgtagg tccgaacgga agtggaaaga gcaatgtcgg
      781 tgatgcggtg agatgggtgc tgggtgaaca gagtgccaga tctctgcgag gaggaaatat
      841 gcaggatgtt attttctccg gtacagagac aagaaaaccc ctgggttatg catcggtggc
      901 gatcacactg gataattcag accataagct tccggttgat tttaacgagg ttacggttac
      961 cagaaggctt taccgttcag gagagagtga atataagatc aacggaagtg cctgcagact
     1021 gaaagatatc aatgagatgt tttatgatac aggtatcggt aaagaaggat attccattat
     1081 cggacaggga cagatcgaca ggatcctaag cggtaaaccg gaagagagaa gagagctttt
     1141 tgatgaggca gcaggtattg ttaaatttaa gagaagaaag aataccacga tcaaaaaact
     1201 ggaggaagaa cgccagaacc tggtcagagt gacagatatc ctctcggagc ttaccagaca
     1261 gctggaacca ctggagaaac agtcagagac agcaagagtt tatctttcca aaagagagac
     1321 gcttaaggaa ctggatgtga atatgttcct gatggagtat gctgctgcag ccaaagaact
     1381 caaggaactg gaagagaaga acataattgc gcagaatcag ctgaaggata cccagagttc
     1441 ctatgaccgg accaaggtgg aatatgaacg tctggaacag gagctggaag atttaaattc
     1501 caaaatggat gcacttcgca tttccggtca ggagcaggca atccgtaaac aacagctgga
     1561 aggccagatc aatgtattaa atgagcagat actggcaggg gcacagaatg aagagcatta
     1621 taagggacgt atccagacta ttgaggctga actttcagta agaacagatt caaagaagaa
     1681 acttgaagaa gaaaaaacgg atatttatgc acagttaaag gctgtccgta agaaactttc
     1741 tgaggaagaa gagaaattaa ggacagcgca ggagaatatg gaagcctgta ctctcgaagt
     1801 ggagaatggc aaaaatgaga ttattgaaat tttgaacagc cgcgccaatg tcaagggtaa
     1861 agcccagaga tttgatgcaa tgatggaaca ggcagaaatc cgtaaggcag agatcagcca
     1921 gcgtattctc agactgaaga gtgaagagga agaacagcag accattctga ctacagcccg
     1981 gaaacagtat gatgagatta catcccagat tgaaaatgcc aatgaggaat gtgaacatct
     2041 gaatctgagt gtgatgaaga ttcaggaaaa gctgaaggaa cagaacacaa agttggaagt
     2101 agaacagaca gcctatcaca gagaggcatc ccggctggat tctctcagaa atatcgcgga
     2161 gcgctatgat ggatacggaa acagtatccg gagagttatg gaacaaaaag aacgtgttcc
     2221 gggaatccag ggtgttgtcg cagatctgat ccaggtaaac aaagattatg agatcgctat
     2281 cgagacagct cttggtggga gcattcagaa tattgttaca gataatgagc agactgccaa
     2341 aactatgatt gaatttctga aaaagaatcg ttatggacgt gcgacctttc ttcccatgag
     2401 cagcataagt cccagaggtg agtttacacc gaaagaagca ctgaaagagc cgggcgttgt
     2461 ggggattgcc agcgaactgg tgtccgtagc ttctcagtat cagcagatca cgaaattcct
     2521 tcttggaaga gtgctggtag ttgataacat tgatcatgcc attgcaatag gaaaaaaata
     2581 cagacacagt ctgagaatgg tcacaacaga gggtgaatcc ttaaacccgg gaggttccat
     2641 gaccggcggt gccttcagaa acaacagcaa tctgcttgga agacggcgtg aaatagagga
     2701 actggaaaca aaagtaaatc agctgaagca gaatctgacc gaaatgcaga atgctgttga
     2761 ggaaaataaa aatcagagaa acaggcttcg tgatgctatt gcaggatttc aggaaaaact
     2821 ccgtcagaaa tatatagaac agaatactgc cagaatgaat atcaaactgc aggaaaagaa
     2881 agctgaggag atccgaagcg gatatgcgca gatcaacaga gatcaggcag agatcaaacg
     2941 ccaggtcatg gaaatccggc aggatcatga ccggattgcc agggaactgg agaattcaaa
     3001 gcaggatgag aaagaactgg agagttttat tgaggcaaag cagtcagaac tggatgaatg
     3061 gaaagaagaa gagaagaaaa tcacccggga gttagaagaa atccgtcttc agtcatccgc
     3121 tcttgaacag aaagagaaat ttgaccagga aaacctgagc cgtctgaaag cagaaatcac
     3181 agcttttatg actgagaaag aggatatt
//
LOCUS       DBMDXG010000259         3207 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6767_length_3207_cov_1.534771, whole genome shotgun sequence.
ACCESSION   DBMDXG010000259 DBMDXG010000000
VERSION     DBMDXG010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6767_length_3207_cov_1.534771"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(172..775)
                     /locus_tag="ACZPD3_07500"
                     /pseudo
     CDS             complement(172..775)
                     /locus_tag="ACZPD3_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569811.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
     gene            complement(803..1555)
                     /gene="dprA"
                     /locus_tag="ACZPD3_07505"
     CDS             complement(803..1555)
                     /gene="dprA"
                     /locus_tag="ACZPD3_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1415871.1"
                     /translation="MIYWIWLTQLPFIGPVTARYLIEELKDAENIYKADAGHLAEIPG
                     LSEQQRRSILQNHSLELPKKIFEDCQNNNISILCWNDKRYPVRAKKPADAPVVLYYKG
                     NFKNMDHTVGIVGARRCSQESKQKTVALASEYAQKQVTVVSGMAKGIDSYAHTACLNA
                     GGYTVAILGNGLDICYPSEHNKLMKCIEEKGLLISEYQPGIQPDKYRFPRRNRLIGSW
                     SDKLFVIEAGRGSGALITAEYSRKYGREVEMM"
     gene            complement(1552..3096)
                     /locus_tag="ACZPD3_07510"
     CDS             complement(1552..3096)
                     /locus_tag="ACZPD3_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1415872.1"
                     /translation="MATVVRSFAIQGIDGYPVDIEIKMLEGQPSISIIGLGDQAVKEA
                     AERIQAAIDESGYVFPKKRVIISLAPSDKKKSGSHFDLAMAVGVLCQNGDIGVKKLPD
                     YGFIGELSLDGRLRVCRGILPMIIAGQKRGIKKIIVPEANFKEASLVHGVEILGFYDL
                     TGVIRYLEGKTTGIETHMEENEKILEESERILDFKDVKGQDELIEAVVLAAAGGHNML
                     MVGEPGCGKTMIAQRIPTILPEMTEEECLEVTKIYSISGLLPNEHALMKYRPFRAPHH
                     NASLNALIGGGANAMPGEVSLAHNGVLFLDELAEFSRRTLDALRQPIEDKKVSISRVN
                     GTHTFPSNFMFITAMNPCPCGYYPSAKCKCTDYEIVKYRGKISGPIMDRIDIQKEVHP
                     VDFFAIADQKPCCSSEELRLKVKKARKIQQERYAQESGINCNAQMTTEMIQKYCALDP
                     DSLNILRETSEKYGYSARVIHKLLRLARTSADLDGEEKIRQKDIEKVLICRELDKSNS
                     KMVVVK"
BASE COUNT          832 a          829 c          486 g         1060 t
ORIGIN      
        1 ttttagttat atttatgatt tttcattatt atatccacat tttttaaaat taatcaaaca
       61 taaatcttat aatataaatt gtataacgag tataaatata aaataacata gctgaaagag
      121 aatatcaaga aaaaacattc gacaaaaaga tccgtctctg ctcttcaatc tatccctacc
      181 atacaaatcc ctggtataca ccttctcctt cacatcatcc agacagctgt catacctgtt
      241 cgcgataata gcctgactca actctttaaa cttctccaga tcattcacca ttctgcttcc
      301 aaagaacgta cttccatcct ccagtgtcgg ctcataaata accaccgttg cacctttcgc
      361 cttaatacgc ttcatagctc cctgaataga agactggcgg aagttatcag aatttgattt
      421 catagtcaga cgataaacac caaccacaac ttccttctct ttacttgcat cgtaatcatc
      481 attcgcccca taagcccctg tcatttccag cacacgctct gcaatataat ccttacgtgt
      541 tctgttcgac tccacgattg cggacatcat gttctgaggt acatccgcat aattagccag
      601 aagctgcttt gtatccttcg gcaggcaata tccgccatat ccaaaagacg gattattatg
      661 tatccagttc attaaaataa gacacacgca aagccagata tgtattcgca aacagcttca
      721 cagcctcagc ctctgtaaat cccatgatca aagtatcaat atttttctta atagcctctc
      781 ctgcagaagt gtcaaaaatt tttcacatca tttctacttc acgcccatat ttcctgctat
      841 attctgctgt tatcaacgct ccgcttcctc tccctgcttc aattacaaat agtttatccg
      901 accagctgcc aatcagacga tttctgcgtg gaaatctgta tttgtcaggc tgaattcctg
      961 gttgatattc tgagataagc agaccttttt cttctataca tttcattaat ttattatgct
     1021 cacttggata gcagatatct aatccatttc ccagtattgc cacggtatag ccgcctgcat
     1081 tcagacaggc agtatgcgca tagctgtcaa ttccttttgc catgccgctg acaactgtaa
     1141 cctgtttctg tgcatattct gaagcaaggg ctactgtctt ttgctttgat tcctggctgc
     1201 atcttcttgc tcctactatt ccaaccgtat gatccatatt cttaaaattc cctttatagt
     1261 ataatactac gggcgcatcg gccggtttct ttgcacgtac aggatatcgc ttgtcattcc
     1321 agcataaaat agaaatatta ttattctggc agtcttcaaa aattttcttt gggagttcca
     1381 atgaatgatt ctggagaatg cttcttctct gctgttcaga taatcccggt atttcagcca
     1441 gatgccctgc atctgctttg taaatatttt ctgcatcttt caattcttca attaaatatc
     1501 tggctgtgac gggtccaata aacggaagct gggtaagcca tatccaataa atcatttaac
     1561 caccaccatt ttactgttgc tcttatccag ttcccggcat atcaggactt tttctatatc
     1621 tttctgacgt atcttttctt ctccatccag atccgcgcta gttctggcaa gtcttagcag
     1681 tttatgtatt actctggcac tatatccata tttctcactg gtctctctta aaatattcaa
     1741 tgaatctgga tcaagagcac agtatttctg gatcatctct gttgtcatct gtgcattaca
     1801 gttaatcccg ctttcctgtg cataccgttc ctgttgtatt ttcctcgcct tttttacctt
     1861 taaccgcaat tcttctgagg aacagcatgg cttctggtct gctatcgcaa aaaaatccac
     1921 tggatgtact tctttctgta tatctatcct gtccatgatc ggtccggaaa tctttcccct
     1981 gtatttaaca atctcataat ctgtacattt gcatttcgcg cttggataat acccacacgg
     2041 gcatggattc attgccgtta taaacataaa attcgaaggg aatgtatgag taccatttac
     2101 tctcgaaata gatactttct tatcctctat cggctgccgc agggcgtcaa gagtccttct
     2161 tgaaaattca gcaagttcat ccaaaaacag tactccatta tgtgccaggg atacttcacc
     2221 cggcattgca tttgctccac cgccaatcag tgcatttaac gatgcattat gatgcggcgc
     2281 acggaacggc ctgtatttca tcagggcatg ctcatttggc agtagtccgg aaatactata
     2341 tatctttgtt acctcaagac attcctcttc tgtcatttct ggaaggattg tagggattct
     2401 ctgtgcaatc atcgtttttc cgcagccagg ttcccctacc atcaacatat tgtgaccacc
     2461 tgctgccgca agcacaactg cctctattaa ctcatcctgt cccttaacat ctttaaaatc
     2521 caaaatcctt tctgattctt ccagaatttt ttcattttcc tccatgtgcg tttctatccc
     2581 tgtcgtcttt ccctcaagat agcgaatcac cccagtcaaa tcatagaatc ccagaatctc
     2641 caccccatgc accagagatg cctctttaaa attcgcttcc ggaacaatga tcttctttat
     2701 tccccttttc tgtcctgcta tgatcattgg caggattccc ctgcataccc tcagtcttcc
     2761 atccagtgat aattctccga taaacccata gtctggcagt ttttttacac cgatatctcc
     2821 attctgacac agcaccccta ctgccatcgc aaggtcaaaa tgagatccac ttttcttttt
     2881 atcactgggt gcaaggctta tgatcacacg tttcttcgga aaaacatagc cactctcatc
     2941 aattgccgcc tggattctct ctgctgcttc ctttacagcc tgatcaccaa gaccaatgat
     3001 tgatatcgaa ggctgtcctt ccagcatctt tatttctata tccacaggat atccgtctat
     3061 cccctgtatc gcaaaacttc ttaccacggt agccataatt catcttctcc catcatcttc
     3121 ctgatctgct gttcctggac aatatattta tgtccgatat cttctacgaa ttccctgtaa
     3181 cgaaatatac tgtttttcgg gaaatat
//
LOCUS       DBMDXG010000260         3188 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6812_length_3188_cov_1.460641, whole genome shotgun sequence.
ACCESSION   DBMDXG010000260 DBMDXG010000000
VERSION     DBMDXG010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6812_length_3188_cov_1.460641"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            227..1282
                     /locus_tag="ACZPD3_07515"
     CDS             227..1282
                     /locus_tag="ACZPD3_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1415873.1"
                     /translation="MITIKEMAEMLGISTTTVSNVINGKTSEVSQKTAEKVQKLLDEY
                     DYVPNMNAKNLAQNHSRLIGIVLKRRKDKYENIFTDPFHGELLGALESAIREQGYYMM
                     IYISEDIEEIVRNIVGWNTEGLILIGMLHDDYLKIRSKYKKPAVLIDSYTPKNIARYV
                     NIGLDDEEGGYLMTKYLLDCGHRKIAFLADNMEGVDYIRYTGHQRALQEYGLDIDLDN
                     LIVIRPSKYERQGSMEEIYAVAHKFTAFMCCSDYYAVTLMKYLKAKGIRFPEDLSITG
                     FDDNLYAQLACPSLTTIHQDIFTRGTIAAEYLFKMIDGWNPKTTNLSLPVRLVVRDSV
                     KLLNPPEDDSSDDGSAL"
     gene            complement(1447..3111)
                     /locus_tag="ACZPD3_07520"
     CDS             complement(1447..3111)
                     /locus_tag="ACZPD3_07520"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HIR1415874.1"
                     /translation="MVKKWWNEKVAYQIYPKSFYDTNGDGIGDLRGVIEKLDYLKDLG
                     VDIIWLSPCYRSPLADQGYDISDYYDIDPRFGTMADMDELIAEAKKRDMYILMDLVVN
                     HCSDEHEWFKKACEDPDGKYGNFFYLRDKKEGELPTNWRSYFGGPVWEDLPGTNKQYL
                     HVFHKKQPDLNWENPEVREEVYKNINWWLDKGLGGFRIDAIINIKKALPMHDYEPDRE
                     DGLCSIRKMLKEAKGIGDFLGEMRDRTFKKYDAFSVGEVFDEKDEEIPDFIGDNGYFS
                     SMFDFEETIWGASDKGWYDCKQITPDAYKKCCFTTQRKIGDIGFVSNIIENHDEPRGV
                     SRYIPEGDCCDASKKMLGGLNFMLRGLPFIYQGQELGMENVKFESIDQVDDISSLDEY
                     KVALEAGCTPEEALKAVSRFSRDNARTPMQWTDGENAGFTTGKPWLKVNANYTKINAE
                     SQMNDPESVRSFYKKLIALRKDPEYKETVVYGELEPVWEDVHNLMAYYRKGDKTLLVV
                     GNYQKEPQTIELAGECRKVLINNYDNVAMDGNKIELQGYQFLVIEM"
BASE COUNT          906 a          751 c          568 g          963 t
ORIGIN      
        1 aatcgtaatc cagaaataat attccgtgca gggctgttgt cagatatttt ttgacgacag
       61 ccttatattt tgcatttttt aattattatc actctctccc aaacgactta tgagcaacag
      121 ttacaatttt cttttatttt attcaaactt ttttgtgcaa cacttgcatt ttcatgtccc
      181 aatgtgataa tatatttata tatttttgta ttgaggtgaa tctcacatga taacaattaa
      241 agaaatggca gagatgcttg gaatcagtac caccacagta tcaaatgtca ttaatggaaa
      301 aaccagcgaa gtttcccaga aaactgctga gaaagttcag aaacttctgg acgaatatga
      361 ttacgtccca aatatgaatg caaaaaatct cgcacagaac cattccagac tcatcggaat
      421 tgtattaaaa aggcgaaagg ataaatacga gaatatcttc acagatccat ttcacggcga
      481 actgctggga gctctggaat ctgctattcg tgagcagggg tattatatga tgatttatat
      541 ttctgaagat attgaagaaa ttgtgcgcaa tatagtaggc tggaacactg aaggactgat
      601 tctcatcggt atgctccacg acgattatct caaaatcaga agtaagtaca aaaaacctgc
      661 tgtccttatc gacagttaca ctccaaagaa tattgccaga tacgttaata tcggtcttga
      721 cgatgaagaa ggcggatacc tgatgacaaa gtatcttctt gactgcggtc acagaaaaat
      781 cgcatttctt gccgacaata tggaaggtgt agactatatc cgatacaccg ggcatcagcg
      841 ggcacttcag gaatacggac tggatattga tctggataat ctgatcgtga tccgcccttc
      901 caaatatgaa cgacagggaa gtatggagga gatttatgca gtcgcacaca agttcactgc
      961 ctttatgtgt tgttccgatt attacgcagt taccctgatg aaatatctca aagccaaagg
     1021 gatccgcttt ccggaagatc tctccatcac cggatttgac gacaatctgt atgcgcagct
     1081 tgcttgtcct tcacttacca ctattcacca ggatatcttt acgcgcggaa ccattgccgc
     1141 agaatatctg ttcaaaatga tcgatggctg gaatccaaaa acaacgaacc tttctcttcc
     1201 ggtcagactt gtagtacgtg acagcgtaaa gcttctgaat ccccctgaag atgattcctc
     1261 cgatgacggt tcagctttat aaggcactca gggatatctg tcgcgggatt tcacgctccg
     1321 atgagccagg agcaatatgc attatttacg aggattgcta tgagaagatt gctataattc
     1381 atagatacaa aaagaacgaa aatccaggta catatcatac ctggatttcc gttctttttt
     1441 cttctgctac atctcaataa caaggaactg ataaccctgc agttcaatct tatttccatc
     1501 catagctaca ttgtcatagt tattgatcag aaccttcctg cactctcctg caagttcaat
     1561 ggtctgtggc tctttctggt aattaccaac tacaagaaga gtcttatccc ctttacgata
     1621 gtatgccatc agattatgta catcttccca tacaggttcc agttctccgt aaacaacagt
     1681 ctctttgtat tccggatcct tacgcagtgc gataagtttc ttatagaaac ttctcacaga
     1741 ttccggatca ttcatctggc tctctgcatt gatctttgta taattcgcat tgaccttcag
     1801 ccatggttta cctgtcgtaa atcctgcatt ctcaccatct gtccactgca tcggtgttct
     1861 ggcattatcg cggctgaatc tggaaacagc tttcagcgct tcctcaggtg tacatcctgc
     1921 ttccagagct accttgtact catccagact ggaaatatca tctacctgat cgatagattc
     1981 aaacttcaca ttctccatac caagttcctg tccctggtag ataaacggaa gtccgcgaag
     2041 cataaagttc agaccgccaa gcattttctt acttgcgtca cagcagtcac cttccgggat
     2101 atatctgctg acaccacgag gctcatcatg attttcaata atattggaaa caaatccgat
     2161 atcaccaatt tttctctgtg ttgtaaagca acatttcttg taggcatccg gagtaatctg
     2221 tttgcagtca taccatccct tgtcagatgc cccccagata gtctcttcga agtcaaacat
     2281 actggagaaa tatccattat cgccgatgaa atccggaatc tcttcatcct tctcatcgaa
     2341 aacttcaccg acagagaatg catcgtattt cttaaaggtg cggtctctca tctcgccaag
     2401 gaagtcgccg attccctttg cttctttcag catcttcctg atactgcaaa gaccgtcttc
     2461 acgatccggc tcatagtcat gcatcggaag tgctttcttg atattgataa tcgcatcaat
     2521 acggaatcca ccaagacctt tatccagcca ccagttgata ttcttataaa cttcttcccg
     2581 tacttccggg ttctcccagt tcaaatctgg ctgtttctta tggaaaacat gcagatactg
     2641 tttatttgtt cctggaagat cttcccatac aggtccgccg aaataagaac gccagttagt
     2701 tggaagttct ccttctttct tgtcacgcag atagaagaaa ttaccatatt taccatccgg
     2761 atcctcacat gcttttttaa accattcatg ttcatcagaa cagtggttta caacaagatc
     2821 catcagaatg tacatatctc tcttcttagc ctcagcaatc agttcatcca tatctgccat
     2881 ggttccgaag cgaggatcaa tatcataata atctgaaata tcatatccct gatcagctaa
     2941 cggagagcgg tagcaggggg aaagccagat aatatcaact cccagatctt tcagatagtc
     3001 aagcttctca ataacacctc tcagatctcc aattccatct ccattggtat cataaaaact
     3061 cttcgggtaa atctgataag caaccttttc attccaccat ttctttacca ttgtaaaaat
     3121 cctcctgtgt tttttattaa ccggcgctat tgtctgcttt gtccccggta ttatacctct
     3181 taccttta
//
LOCUS       DBMDXG010000261         3185 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6819_length_3185_cov_1.435149, whole genome shotgun sequence.
ACCESSION   DBMDXG010000261 DBMDXG010000000
VERSION     DBMDXG010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6819_length_3185_cov_1.435149"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="ACZPD3_07525"
     CDS             <1..367
                     /locus_tag="ACZPD3_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR1415875.1"
                     /translation="LILEGEDRLWRNRETREHDNCISYSCISEEWCELADKRQCLLER
                     MIAEETVRELMNLLTERQRQMIHRIYFQQHTQKEVSHELGITVPAVSKCISQAIQKMK
                     NNSDDSARALEKGDWKYAW"
     gene            357..779
                     /locus_tag="ACZPD3_07530"
     CDS             357..779
                     /locus_tag="ACZPD3_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR1415876.1"
                     /translation="MPGKKPKERQRYMLRVNDTLVEVTRAVYLAWYQAGRKERYQVEK
                     MQRHGVCSMEELQEKGYDCSFSILSPEEIVIRMSEIQELEEAMRCLAKEEAELITLLF
                     FEEFTVKETAQYFGCCPKTIRNRRKKVLEKLKEQLEKL"
     assembly_gap    1278..1287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1288..>3185
                     /locus_tag="ACZPD3_07535"
     CDS             <1288..>3185
                     /locus_tag="ACZPD3_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HIR1415877.1"
                     /translation="ASELETEKIPFEQQYAELKDVQDSLDPNEIVKANDIELSYGQEF
                     DVEVDLSGIEGVDESKIKILFHEAKNEAGTDFDTHTPDTYKAVYAVEPVSGHPAYRVS
                     RNITVKEPKTEAITTSNSSENTTDEGNAGETEDIGTADDEETDRQTETEVVTDLTEES
                     ETTTDEETGLTVSDVMEQADEEGIDLYSLDEGESVTFMARVASTTSTKKVTVTRGACY
                     QYSDYGYGSYLTYQYTVKFGSVSATAYCIQPEKSSPGSGTYDITKLSDGKKLAKVCYY
                     GTKASGDDGFFTEENGYGNLSTGARFILVHLAASYANSGDSAFSGASSKAKTLAMKLY
                     NYCISQPNIPDVEMSFSDANVTAYVDGSSQRTKEITFKADELQSITMKLPSGVKLHNV
                     TTGKTSKAGESVVISGGTKFYLSAPLTQVSDVAASWSVTMKGSITKDYSAYKISTGSG
                     SQDLALVFGEGVDDEKYVDFKVTWVQYASVKVIKKDSKANVKLSGAVFGLYSDADCKN
                     LITKLPATDANGEASAQIVKTQDTVYLKEITAPSGYRINATAYNVKLEVSKTTTVTVP
                     DEEQLGQLTVYKEGEVLTGADVTENGTTFRYEKRRQKGAVYNVYAGADTTTAYGTKVY
                     SKGDLVNE"
BASE COUNT         1089 a          520 c          782 g          784 t
ORIGIN      
        1 gctgatatta gaaggcgaag atcgtctgtg gagaaacagg gaaacacgag aacatgataa
       61 ttgcatttcg tatagctgta tttccgaaga atggtgcgaa ttggcagata aacggcaatg
      121 tctgcttgaa agaatgattg cagaggaaac agtcagggag ttgatgaatc tgttgacaga
      181 gcgacagaga cagatgatcc atcgtattta ttttcagcag catacacaaa aagaagtctc
      241 gcatgaactg ggtattacgg ttccggcagt atcaaagtgt atttcacagg caatacagaa
      301 aatgaagaat aattcggatg attctgccag agcattggaa aaaggagact ggaagtatgc
      361 ctggtaaaaa gccaaaagaa agacaacgct atatgctgcg tgtgaatgac acgcttgtgg
      421 aagttaccag agcagtatat ctggcctggt atcaggctgg aagaaaagag cgataccagg
      481 ttgaaaaaat gcagaggcat ggagtatgca gcatggagga attacaggaa aaaggctacg
      541 actgctcttt ttcgatatta tcaccggaag aaatcgttat caggatgtca gagatacagg
      601 aactcgaaga agctatgaga tgtttagcta aagaagaggc agaactgata acgctattgt
      661 tttttgaaga atttacagta aaagaaacag cacagtattt tgggtgttgc ccaaagacaa
      721 tcagaaaccg gagaaagaag gtattggaga aattaaaaga acaactggaa aagctataaa
      781 aacatattgg aataaaaagt tctaaaaatt ttttcaaaca ggttaatttt tgccctttca
      841 cacatgcggt tagtgaaggg gcaaatgtct attttcaatt cgagataata actcccctaa
      901 aaattgaacc ttgacaattt cataccagca gcgagaggat acccggaatt tttcagctac
      961 gcattcttgc ggcgggaatc cggtgagaac gtgaagggat acagatggga ggcacccttt
     1021 ataggaaaga ttacgaatta cctcttgcgg cttaatgctt aatcctgaat tttatccaag
     1081 tgtcatttct gctttgcaga atgacagtaa tgagaggtgg gtggtggttc aaatgattga
     1141 acaatgcagg tgtgagcctg cgtgacaaca gaacagataa tagaagaaag agaggattcg
     1201 atcaaaatga aaatgagatt caaaagattc ttatccggtt ttatggcagt tgcaacattg
     1261 gcttctacca ttattcannn nnnnnnnagc ttctgaatta gaaacagaaa aaataccatt
     1321 tgaacagcag tatgcagaat taaaagatgt tcaggattcc cttgatccga atgagatcgt
     1381 aaaggcaaat gacattgaac tttcttatgg gcaggaattt gatgtagaag ttgatctgtc
     1441 tggaatcgaa ggtgtagatg aaagtaagat caagatttta ttccatgaag caaagaatga
     1501 agctggcaca gattttgata cacatacacc agatacttac aaagctgtct atgcagtgga
     1561 accagtcagc ggtcatcctg cttatagagt cagcagaaat attacagtga aagaaccgaa
     1621 aacagaagct ataacaacgt caaatagttc tgagaatact acggatgaag gtaatgccgg
     1681 agaaacggag gatattggaa ctgcagatga tgaagagacg gacaggcaga cagaaacaga
     1741 agtagtgact gatctcacag aagaatcaga aacaacgaca gacgaagaaa ctggattgac
     1801 agtttctgat gtgatggaac aggctgacga ggaagggatt gacctttatt ctttagatga
     1861 aggggaatca gttactttta tggcaagggt agcatctacg acaagcacca agaaagtaac
     1921 agtgacaaga ggagcctgct atcagtattc tgattatggc tacggaagtt atctgactta
     1981 tcagtatact gtaaagtttg gaagtgtttc tgctactgct tactgtattc agccggaaaa
     2041 atccagtcca ggaagtggaa cttatgatat taccaagctg agtgatggta agaaacttgc
     2101 gaaagtctgc tattacggaa caaaggcatc cggtgatgat ggatttttta cagaagagaa
     2161 tggctatgga aatctgagca caggagcaag atttatcctg gtgcatctgg cagcatctta
     2221 tgccaacagc ggagattctg ctttttccgg tgcaagcagc aaagcaaaaa cacttgctat
     2281 gaaactgtac aattactgta tttcgcagcc aaatattcca gatgttgaga tgagcttttc
     2341 agatgcgaat gtaactgcct atgtggatgg cagcagccag agaacaaagg agattacttt
     2401 taaggcagat gaactgcaga gtatcacaat gaaacttcca agtggagtaa agcttcataa
     2461 tgtgacaaca ggaaagacaa gcaaggcagg agaatccgta gtgatcagtg gcggaacaaa
     2521 gttctatctg tctgcaccgc ttactcaggt aagtgatgta gcagcaagct ggtctgtcac
     2581 aatgaaagga agtatcacaa aagattattc tgcttataag atcagtaccg gttctggttc
     2641 acaggatctg gcattggtgt ttggtgaggg tgtcgatgat gaaaagtatg tggattttaa
     2701 agtgacatgg gtacagtatg ccagtgtgaa agttattaaa aaggattcta aagcaaatgt
     2761 aaaactgtcc ggagctgtat ttggcttata cagtgatgca gactgtaaga atctgattac
     2821 aaaactgccg gcaacggatg cgaatggaga agcatctgca cagatcgtta agacacagga
     2881 taccgtttat ctgaaagaga ttactgcacc aagcggttat cgtattaatg ctacagccta
     2941 caatgtgaag ctggaagtca gcaagacgac tacggtaact gttccggatg aagaacagct
     3001 tggacagctt actgtatata aagagggtga agttctcaca ggtgccgatg tgacagaaaa
     3061 cggaacaaca ttccgatatg aaaaacgccg acagaaaggt gctgtttata atgtttatgc
     3121 cggagcagat actacaactg cttatggtac aaaagtttac agcaaaggtg atctggtaaa
     3181 tgaga
//
LOCUS       DBMDXG010000262         3178 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6839_length_3178_cov_1.728307, whole genome shotgun sequence.
ACCESSION   DBMDXG010000262 DBMDXG010000000
VERSION     DBMDXG010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6839_length_3178_cov_1.728307"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            303..1775
                     /gene="malQ"
                     /locus_tag="ACZPD3_07540"
     CDS             303..1775
                     /gene="malQ"
                     /locus_tag="ACZPD3_07540"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR1415878.1"
                     /translation="MRKAGVLMPVSALPSRIGAGELGESAFQFIELLKKNHVKIWQIL
                     PLNPVGYGNSPYQPYSSCAGDELYISLDALAEEGLLKEHPKEFQERATRVDYEAVRQY
                     REPFLRTAFDVFTEKKGQEETAYKEFASQEWVYEYGVFRALKKANNGECWNDWPEEYR
                     TWPENRQKLPAEVETEAQYQMFLQYIFYTQWMKVKKAANDAGIQIMGDVPFYVGQDSV
                     DVWGGKDNFLLDTDGRPIFIAGVPPDYFSATGQRWGNPIYDWEHMKEQDYRFWVDRIG
                     YSNKLFDIIRIDHFRAFDTYWKIPAECPTAIDGEWIEAPGYEVIDTLQKEIPGLDLVA
                     EDLGLLRPEVLMLKDHYHLKGMKILIISIETGGKYARDTFHDVENMIFYTGTHDNDTI
                     MQWYGNMSAAARRKIRRMLKKAGASQGSVKDRFLQYTMQNQAEYAIIPLADILGLGKE
                     GHINTPGTIGSPNWEWHLPDFIQAKKELQKFGRLIVDTKR"
     gene            2160..>3178
                     /locus_tag="ACZPD3_07545"
     CDS             2160..>3178
                     /locus_tag="ACZPD3_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR1415879.1"
                     /translation="MNMFSKVFGTRSQREVKRIMPLVEKIESLRPDMQKLSDEELRGK
                     TREFKKRLEEGETLDDLLPEAFAVVREAGKRVLGMEHFRVQLIGGIILHQGRIAEMRT
                     GEGKTLVATLPSYLNALEGKGVHVVTVNDYLAKRDAEEMGKIHEFLGLTVGVVLNDMK
                     QDERRAAYNCDVTYVTNNELGFDYLRDNMVIYKEQLVQRDLHYCIIDEVDSILIDEAR
                     TPLIISGQSGKSTKLYEACDILAQQLERGEASHEMTKMAAIMGEEVIETGDFVVNEKD
                     KIVNLTEQGVHKVEKFFHIENLADPENLEIQHNITLALRAHNLMHKDQDYVVKDDEIL
                     IVDEFT"
BASE COUNT          996 a          567 c          799 g          816 t
ORIGIN      
        1 aaggcttcct tacagagaga caaaatgccc tctgtaaggg agcttttttg tcaggaaaaa
       61 tatatacctt tagcgcataa ggaatgcttt gacgcagtgg cctaaatatg ttaaaatatc
      121 aaaagatatt atgaaatttg taactgatca gcagttaaaa aattacaagt tgaaattcat
      181 tccggattgc ctgcggcaat ggaatattga cttgtatatt ttgttggaat ggattgctga
      241 agaatagtta ccgaaatttt ataaatgaag aagtgtaaaa agaaggaagg agcacaaaaa
      301 tcatgagaaa agccggagta ttaatgccag tttccgcact tccgtcccgt attggtgccg
      361 gagaacttgg agaatctgca ttccaattta tcgagttatt aaagaagaat catgtaaaga
      421 tatggcagat actgccactg aatccggttg gctatggtaa ttccccatac cagccatatt
      481 cctcctgtgc aggggatgag ttgtatatca gtcttgatgc gcttgcagag gaaggtctgt
      541 taaaggaaca tccaaaggaa tttcaggaga gagctacaag agtagattat gaggctgtcc
      601 gccagtacag agagcctttc ttaagaactg cgtttgatgt ttttacagag aaaaagggac
      661 aggaagaaac agcatataaa gaatttgctt cacaggaatg ggtatatgag tatggtgtgt
      721 tccgcgcatt aaagaaagca aataacggag aatgctggaa tgactggccg gaagaatata
      781 gaacctggcc ggagaacaga cagaagttac cggcagaggt ggagacagag gcacagtatc
      841 agatgtttct tcagtatatt ttctacacac agtggatgaa ggtgaagaag gctgcaaacg
      901 atgctggaat ccagattatg ggagatgttc ctttctatgt gggacaggat tctgtagacg
      961 tatggggtgg aaaagataac tttcttcttg atacagatgg acgtccgatc ttcatcgccg
     1021 gagtaccgcc tgattatttc agtgctacag ggcagagatg gggaaatccg atctatgact
     1081 gggagcatat gaaggaacag gattacagat tctgggtgga cagaattggc tatagcaaca
     1141 aattgtttga tattataagg atcgaccatt tccgggcatt tgatacatac tggaaaattc
     1201 cggcagaatg cccgactgct attgacggtg aatggatcga ggcaccggga tatgaggtca
     1261 ttgatacact tcagaaagaa attccgggat tagatctggt agcagaggat ctggggctgc
     1321 ttcgtccgga ggttctgatg ctgaaagatc attatcatct gaagggaatg aagatcctga
     1381 tcatctccat agaaacaggt ggaaaatatg cccgagatac cttccatgat gtggaaaaca
     1441 tgatttttta cacaggaacg catgacaatg atacgatcat gcagtggtat ggaaatatga
     1501 gtgccgcagc cagaagaaaa atccgcagga tgttaaagaa ggcgggagca tcacagggct
     1561 ctgtcaagga cagattcctc cagtatacca tgcagaatca ggcagaatat gcgatcattc
     1621 cgttggcaga tattcttgga cttggaaaag agggccatat caacacaccg ggaaccattg
     1681 gttctccgaa ctgggagtgg catttaccgg attttataca ggccaaaaag gaactgcaga
     1741 aatttggcag actgatcgtg gatacaaagc gataaatttg ttactgttca cacaccacta
     1801 tcccgcgagg tgttttggta tgaatatgcc aaaatcccga gacatacaag ccaaaattcc
     1861 attgccgtag gcaatctgga atgaattttg gctacgttac tgtgaacgga gtgaacagta
     1921 acgtaaattt tggtgactcc gtaattttct tggtttgttc atgaaaaatt cattgttttc
     1981 cataacggaa agtgttagaa tatacaagta ttgtgtatta tagtaagcgt ttgtaatgtt
     2041 cgcatgctgc tgacatgtct gctgtggacg gagtgaacag aaattattcc gcacagtagc
     2101 aattgttata tggcagcaat gcttgtataa aaaagaaata aacaggagta aaacctaata
     2161 tgaatatgtt ttcaaaagta ttcgggaccc gcagccagcg agaggttaag cgtatcatgc
     2221 ctctggttga gaaaattgag agcctgcgcc cggacatgca gaagttaagc gacgaagaac
     2281 tcagaggaaa aacaagagaa tttaagaaac gtcttgaaga gggagagaca ctggatgatc
     2341 tgcttcctga ggcatttgcg gttgtacgtg aagcaggcaa gcgtgttctt ggaatggaac
     2401 atttccgtgt gcagcttatc ggtggtatca ttctccatca gggacgtatc gcagagatga
     2461 gaacaggtga aggtaaaaca cttgtcgcca cacttccgtc atatttaaat gcactggaag
     2521 gcaaaggtgt gcatgtcgta acagtcaacg attacctggc taaacgagat gcggaagaaa
     2581 tgggaaagat ccatgagttt cttggcctga cagtcggtgt tgttttaaat gatatgaagc
     2641 aggatgagcg tcgtgctgca tacaactgtg atgttaccta tgttacgaac aacgaactcg
     2701 gtttcgatta tctgcgtgac aacatggtta tctataaaga acagctggta cagagagatc
     2761 tgcactactg tatcatcgat gaggtcgact ccattctcat cgacgaggca cgtacccctc
     2821 tgatcatttc cggacagagc ggtaagtcaa ccaagttata cgaggcctgc gatatccttg
     2881 ctcagcagtt agagcgcggt gaagccagcc atgagatgac caagatggca gccatcatgg
     2941 gtgaagaagt tatcgagaca ggtgacttcg ttgtaaatga aaaagataag atcgtaaacc
     3001 ttacagagca gggcgttcat aaggttgaga aatttttcca tattgaaaac ctggcagatc
     3061 cggagaatct ggagatccag cataatatta cacttgcact tcgtgcccat aacctgatgc
     3121 ataaggatca ggattatgta gttaaagatg atgaaatcct tattgttgat gaattcac
//
LOCUS       DBMDXG010000263         3156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6889_length_3156_cov_1.812439, whole genome shotgun sequence.
ACCESSION   DBMDXG010000263 DBMDXG010000000
VERSION     DBMDXG010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6889_length_3156_cov_1.812439"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            147..1049
                     /locus_tag="ACZPD3_07550"
     CDS             147..1049
                     /locus_tag="ACZPD3_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1415880.1"
                     /translation="MTLNQLRYFCTASRCHSITKAAEELYVTQPTVSVAIRDLEIEFG
                     ISLFYRKGNRLILTEEGEELYNKATYILQYCNELQADYSSMTRVKPPLRIGIPPMLST
                     VFFPELLIAFHDQYPEIAVVLEEYGSVRACNLVQDDTLDLALVNMEQYNIDKFNKALL
                     ANDQVVFCVSDDHRLADKEVVTTKEMSKESLIFFNADSVQNQLLKTRFETDGYVPDIV
                     MRSSQIYTILQLVKTCKYGSFLYSSMTDRFASSGIKGIPLSPPIRIKIGMIWKKGKYI
                     SDNMQKFLNFTKMYYREHPLIQKF"
     gene            complement(1070..>3156)
                     /locus_tag="ACZPD3_07555"
     CDS             complement(1070..>3156)
                     /locus_tag="ACZPD3_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR1415881.1"
                     /translation="KKNLSKDILTGLIIMAVSIPISMGYAQIAGLPAVYGLYGSVFPI
                     ILFGLFSTSPQFIFGVDAAPAALVGAALVEMGIQSGSKQAMAVVPVLTFFVAVWLFVF
                     AFLKAGKLVNYISAPVMGGFITGICTTIILMQVPKLMGGTAGTGELSELAEHIWATLG
                     QINLPSLILGLVSLVILLVMKKILPKFPMVVVLMAAGVLLSYFVPISNWGIRTLDAVE
                     PGLPVWSIPDFSAIGLKEAVIYSLSIAVVIMAETLLGENSFAQKNSYRVNANQELVAF
                     SMGNLAAALTGCCPINGSVSRTAISEQYQGKTQLTGIVAGISMILVLLGATGLIAYLP
                     VPMLTAIVISSLLGATEFDLAVRLRKISKKECLIYWGAFWGVLTLGTINGVLIGIILS
                     FAEMIIRTAKPARCFLGVQPGHRHFEDLTESLHNCEIEGVVIYRFSSSLFFANIDILQ
                     QDIENAIGSDTKAVILDASGIGSIDVTAADRLAILYRSLKSRGIRFYITEHIAELNEQ
                     MRALGLGFMIEEGRVRRTIHVALKDMGIHYPYPLKGNVDNTERSPFRKRVDNSVQEFV
                     WAFGSDSEAEIEKQIRLQIEQLKENGDMQELLHGQWNHMDELDADAWIEHLEEHMKEI
                     VKISGQDEQELTVRFEKHRKKLREQMMKEHPELVVMFEERRQLLDEQLERKWPDVYAL
                     VLKQREKDSRLK"
BASE COUNT          879 a          802 c          626 g          849 t
ORIGIN      
        1 tgcatatccc cataaaaaat ttttataatc tcaattttag caggtttttt ttatgcaata
       61 ttactacatt aaaaaaaact ttagaaataa atatggaaat ccttataggt atgtggtatt
      121 ttttatttga aaagaggtac ttacttatga cacttaacca actccgttac ttttgcacag
      181 caagccgctg ccacagtatc accaaggcag ccgaggaatt atacgttact cagcccactg
      241 tgtccgttgc tatccgcgac ctggaaattg aattcggtat cagccttttc tatagaaagg
      301 gaaacaggct gatccttact gaagaaggcg aggagcttta taataaagcc acctacattc
      361 ttcaatactg caacgagctt caggcagatt actccagtat gacgagggta aagccccctc
      421 ttcgtattgg gatcccgcct atgctgagca ctgtcttttt ccctgagctt ctcatcgcat
      481 ttcatgacca atatccggaa atcgctgttg ttctggagga atatggctct gtccgtgcct
      541 gcaatctggt acaggatgac actctggatc tggctctggt aaacatggaa cagtataata
      601 ttgataagtt taacaaggct ctcctggcca acgaccaggt tgttttttgt gtaagtgatg
      661 accaccgact ggcagacaaa gaagttgtga ccacaaaaga aatgtctaaa gaatctttga
      721 tctttttcaa tgccgattcc gtacagaatc agttgttgaa aacacgcttt gaaacggacg
      781 gttacgtccc ggacatcgtc atgcgcagca gccagattta taccattttg cagttagtaa
      841 agacctgtaa atatggcagc tttctctatt ccagcatgac cgacagattt gcctcttctg
      901 gcataaaagg aattcccctc agtccaccca tccgcataaa gatcggaatg atatggaaaa
      961 aggggaaata tatcagtgat aatatgcaga aattcctgaa cttcaccaag atgtactacc
     1021 gggaacatcc gctgattcag aagttctgat ttgtacgcca gactgcttgt tattttaacc
     1081 tactatcttt ctcacgctgt tttaagacca gagcatacac atccggccat tttctctcaa
     1141 gctgctcatc caaaagctgc ctgcgctcct cgaacattac caccagctcc ggatgctctt
     1201 tcatcatctg ttcccggagt ttttttcgat gcttctcaaa acgtacggtc aattcctgtt
     1261 catcctgtcc ggaaatcttt acaatttctt tcatatgctc ttccagatgc tcaatccatg
     1321 catctgcatc aagttcatcc atatgattcc actgaccatg gagcagctcc tgcatatctc
     1381 cgttctcttt cagctgttca atctgcagac ggatctgttt ctctatctct gcctcagaat
     1441 cagaaccaaa agcccagaca aattcctgta cactgttgtc cacacgcttt ctgaacggag
     1501 aacgttctgt attatctaca ttgcccttca aaggataagg ataatggatt cccatatcct
     1561 tcaatgccac atgtatagtc cgacgcacac gtccctcttc gatcataaat ccaagtccca
     1621 gtgcgcgcat ctgttcattc agctcagcga tatgctctgt tatgtagaaa cgaattcctc
     1681 ttgacttcag agaacgatac agaattgcaa gcctgtctgc agccgtaaca tcaatgctac
     1741 caatcccgct ggcatccaga atgactgctt tggtatccga tccaatggca ttctcaatat
     1801 cctgctgtag aatatctatg tttgcaaaaa acaggctgct gctgaatcgg tagatcacta
     1861 ctccctcaat ctcacagtta tgcagacttt ccgtaagatc ttcgaaatgt ctgtgtcccg
     1921 gctgcactcc cagaaaacag cgtgccggtt tcgcagtacg gatgatcatc tcggcaaagg
     1981 agagaataat accgatcaat actccattga tggtaccaag cgtcagcact ccccagaatg
     2041 cgccccagta aatcaggcat tcctttttgc tgattttcct cagacgtaca gccagatcaa
     2101 attccgttgc ccccagaagt gaggagatta caattgcagt cagcatagga accggcagat
     2161 aagcaatcag cccggttgct cccagaagca caagaatcat ggaaattcct gcaacaattc
     2221 cggtcaactg tgtttttccc tggtactgtt cactgatggc ggtacgtgac accgaaccgt
     2281 tgatcggaca acagcctgtc agagctgctg ccagatttcc catggaaaag gctaccagct
     2341 cctggttagc atttaccctg taactatttt tctgtgcgaa gctattctca ccaagtaaag
     2401 tttccgccat gatcactact gcaattgaca gactgtagat gaccgcttct tttagaccaa
     2461 tggcagagaa atcaggaata ctccatacag gcagccccgg ttctactgca tccagtgtac
     2521 ggattcccca gttggaaatc gggacgaaat aggataataa tacacctgct gccatcagaa
     2581 ctactaccat aggaaacttg ggcaggattt ttttcatcac cagaaggatg accagagaaa
     2641 ccagacctaa aatcagggaa ggcagattga tctgccctaa tgtagcccag atatgctctg
     2701 ccagctccga caattctccg gttcctgcag ttccgcccat taatttgggc acctgcatga
     2761 gaatgatcgt ggtgcagata cctgtgataa agcctcccat tacaggtgcg gaaatatagt
     2821 ttaccagttt tcctgctttt aaaaatgcaa atacaaacag ccagactgcc acaaaaaatg
     2881 tgagcaccgg cacaaccgcc attgcctgtt tcgaaccact ctggattccc atttccacca
     2941 gtgccgcccc gactaacgct gcaggtgctg catccactcc gaagatgaac tgaggcgatg
     3001 tggaaaacag cccaaacaaa atgatcggga atacagagcc atacagtcca taaaccgccg
     3061 gaagccccgc aatctgggca tagcccatgg aaatcgggat cgagacagcc ataatgatca
     3121 gcccggtgag gatgtccttt gataaatttt ttttgt
//
LOCUS       DBMDXG010000264         3138 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6938_length_3138_cov_1.046427, whole genome shotgun sequence.
ACCESSION   DBMDXG010000264 DBMDXG010000000
VERSION     DBMDXG010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6938_length_3138_cov_1.046427"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="ACZPD3_07560"
     CDS             complement(<1..502)
                     /locus_tag="ACZPD3_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR1415882.1"
                     /translation="MERNKTIMKLCYLQTTFYIVFLTALITRHRRGISQKTFAFLIIL
                     SLVGCAVSLTLGDYLRNMIRSSGFDVAGVHDKKSLEKKLQQLQNADDTLNVGVMMFDL
                     NNLKKINDTYGHEQGDVFIQTFASYLTRILTEDSFLARFGGDEFVIVQNHATWSQLEQ
                     MNIQLQS"
     gene            complement(614..775)
                     /locus_tag="ACZPD3_07565"
     CDS             complement(614..775)
                     /locus_tag="ACZPD3_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR1415883.1"
                     /translation="MEKQRHYLESLGFVPGTQVTILSELHGYYVVMVKGSKIGLDKEL
                     AKKIIVYAG"
     gene            complement(915..1316)
                     /locus_tag="ACZPD3_07570"
                     /pseudo
     CDS             complement(915..1316)
                     /locus_tag="ACZPD3_07570"
                     /inference="COORDINATES: protein motif:HMM:NF027640.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
     gene            complement(1313..2065)
                     /locus_tag="ACZPD3_07575"
     CDS             complement(1313..2065)
                     /locus_tag="ACZPD3_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HIR1415884.1"
                     /translation="MKKADRKKEHFRNEWKYLISTSEKEVLNLRMKPLMKLDPHAENG
                     GYLIRSLYFDDYWNSAYEEKESGVLMRKKYRIRIYNYSAESIKLERKKKFGSYIYKED
                     APLTKDEVQKILSGEYEFLLKSPYNLCREFYTECMSNMMRPRTIVDYEREPWIMDEGT
                     VRITFDTDVRAAVGSYDIFDSTLPALSVLEPGKLIMEVKFTELLPQILRNLLPPQAEE
                     FTAVSKYVLCYEKTRYMNGFEYWYDTQGRMIR"
     gene            complement(2052..>3138)
                     /locus_tag="ACZPD3_07580"
     CDS             complement(2052..>3138)
                     /locus_tag="ACZPD3_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HIR1415885.1"
                     /translation="EQLNKTALKSHGLDSNGQLYKINSFEFYRYEDIIKKEDDAGYDK
                     TAFEKMLEIKGDSDHTKLIDMLTDLNDYSIGIEDVLKEHFDEENIVYWMAFQILMGNV
                     DTQNRNVYLYSPLNSDIWYFIAWDNDGCLMRPEYELRNFSDQNSWEKGISNYWGNILF
                     QRCLKSRSFREKLNDAILDLREYLNKDMLTSKIESYKNVLKPYLYLMPDAEYEPLTSE
                     QYDQIAASLPDEIEKNYQLYLESLETPMPFYIGVPSIDGDKLKFNWDVSYDLDAEDIT
                     YSVEVARDYLFQDVIYQNTTLTVPESEMELPNAGQYFVRVRATNTSGKTQDAFDYYVT
                     DEGKHYGMKCFYITEDSTVEEDNYEEG"
BASE COUNT          887 a          727 c          500 g         1024 t
ORIGIN      
        1 tggattggag ctgaatattc atctgctcca gctgagacca ggtggcgtga ttttgcacga
       61 tcacgaactc atcgcctccg aatctggcga gaaaactatc ttcagtgagt atccgtgtca
      121 ggtaagaagc aaaggtctga ataaacacat ccccctgttc gtgaccatat gtatcgttga
      181 tctttttcag attgttcaga tcgaacatca tgacacccac attcaaggta tcgtctgcat
      241 tctgaagctg ctgcagcttc ttttccaatg attttttatc gtggacgcct gctacgtcaa
      301 agccggaact tcggatcata tttctgagat aatctcctaa agtaagggaa actgcacatc
      361 ccacaaggga taaaataatc agaaatgcaa aggttttctg acttattccg cgtctgtgac
      421 gggtaattaa tgcagtcaaa aatacaatat aaaatgttgt ctgcaggtag cagagtttca
      481 taattgtttt gtttctttcc atacatttct ccagtatata gtgcctgata tttcacttgt
      541 aactatttgg aagacagcat tttgcggagg gcgtaactgt gaagatactg aacagttact
      601 ctctgttttt taattagcct gcgtatacaa taattttctt ggcaagctcc ttatccaggc
      661 cgattttgct gccctttacc ataaccacat aatacccgtg taattctgat aaaatcgtaa
      721 cctgcgttcc tgggacaaag cccagggatt ccagataatg ccgctgtttt tccagtccgc
      781 atagtttttg attttacgaa atattttctt tttccaaaac agcggataat ttcatctccc
      841 gcctgagttc cctgataatg aatcactgca atataaattc ttccattttg ttgtttgtga
      901 taaaacaggg tgatcatcag aatgataatc acagctgtca acagagttaa tgcatacata
      961 cctgcaccgg ctgtaatccc cgatgtgatt gcccagaaca gatacagaag atccatcgga
     1021 tctttaattg ctgtacgaaa acgtacaata gacaatgcac caaccatacc aagagaaatc
     1081 acaatattgc tgctgattgc aagtgttacc atacaggtaa gcactgtcat tcccacaagt
     1141 gtaactgcaa aacttctgga gaaaataact ccaacataaa atttctgata aaccagataa
     1201 atcacacttc ccaaaatcaa agccactgcc agagcagcca atatcttagg tatatcatac
     1261 tgactgaaca ttcctgattc cagtacagat ttttttatca tatcctgtac actcatctta
     1321 tcatcctccc ctgtgtatca taccaatact caaatccatt catgtaccgc gttttttcat
     1381 aacacagtac atatttggaa actgctgtaa attcttctgc ctgcggcggc agaagatttc
     1441 tcagaatctg aggcagcagc tccgtaaatt tcacttccat gatcagtttt cccggttcca
     1501 gtacagataa agccggaagc gtactgtcga aaatatcata gcttccaaca gcagctctga
     1561 catctgtatc aaatgtgatc ctgactgttc cctcatccat gatccatggt tctctctcat
     1621 aatctacgat tgttctcggc cgcatcatat tactcataca ttctgtgtaa aattccctgc
     1681 aaagattata cggacttttc agaagaaatt catactcgcc ggaaagaatt ttttgcacct
     1741 catcttttgt cagcggagca tcttctttat aaatataact gccgaacttc ttttttctct
     1801 ccagcttgat actttcagca ctataattat aaattcgtat cctgtatttc ttcctcataa
     1861 gaacgccgct ttccttttct tcatatgcac tgttccagta atcatcaaaa tacaggctcc
     1921 gtatcagata tcctccattt tctgcatgag gatccagttt cataagtggc ttcatccgaa
     1981 gattcaggac ttctttctca ctggtactga taagatattt ccattcatta cgaaaatgtt
     2041 ctttcttcct gtcagccttc ttcataattg tcttcctcta ccgtactatc ttctgttatg
     2101 taaaagcact tcataccata atgctttcct tcgtctgtta cataataatc aaatgcatcc
     2161 tgcgtttttc cagatgtatt tgttgctctt actctcacaa aatactgtcc tgcattcgga
     2221 agttccatct ccgattccgg aacagtcagg gttgtgttct gatagatcac atcctgaaac
     2281 agataatctc gcgcaacctc cacagaatac gtaatgtcct ctgcgtccag atcataagat
     2341 acatcccagt taaatttcag cttatcaccg tctattgacg gaacacctat ataaaatggc
     2401 attggtgttt ccagactttc cagatataac tgatagttct tttctatctc atcaggaagt
     2461 gatgctgcaa tctgatcgta ttgttcagat gtaagcggtt catactctgc atcaggcatc
     2521 aaatataaat agggcttcag tacattctta tagctctcta tctttgatgt gagcatatct
     2581 ttgttcagat attccctcag atccagaatg gcatcattta atttttcccg gaaagaccgt
     2641 gacttcagac acctttgaaa cagaatattt ccccagtaat tgctgattcc cttttcccag
     2701 ctgttctgat ccgaaaaatt ccttaattca tattccggtc tcatcagaca gccatcgtta
     2761 tcccaggcaa taaagtacca gatatctgaa ttcagaggac tgtataaata aacatttctg
     2821 ttctgtgtgt ctacgtttcc catcagaatc tgaaatgcca tccagtaaac aatattttct
     2881 tcatcaaaat gttctttcag tacatcttca atccctatag aataatcatt cagatctgtc
     2941 agcatatcaa tcaatttggt atgatctgaa tcccctttta tttccagcat tttttcaaag
     3001 gctgtcttat catatcctgc atcatcttct ttttttataa tatcctcgta gcgataaaat
     3061 tcaaaagaat taatcttata caattgtccg tttgaatcca gtccatgact ctttaatgcc
     3121 gtcttattca gttgttca
//
LOCUS       DBMDXG010000265         3130 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6957_length_3130_cov_1.417960, whole genome shotgun sequence.
ACCESSION   DBMDXG010000265 DBMDXG010000000
VERSION     DBMDXG010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6957_length_3130_cov_1.417960"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            10..969
                     /locus_tag="ACZPD3_07585"
     CDS             10..969
                     /locus_tag="ACZPD3_07585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR1415886.1"
                     /translation="MFDFNKPKIEITELSEDKKFGRFVVEPLERGYGTTLGNSLRRIM
                     LSSLPGAAVSQVKIDGVLHEFSSIPGVKEDVSDIIMNLKSLAIKNHSTDNEPKTAYIE
                     CEGKGVVTAADIQADQDIEIMNPDQVIATLNGGKDCRLAMELTITKGRGYISADKGKS
                     DDMPIGVLAVDAIYTPVDRVNMTVENTRVGQVTDYDKLTLDVYTNGTLDPDEAVSLAA
                     KVLSEHLSLFIDLSENAKTAEVMIEKEDNEKEKVLEMSIDELELSVRSYNCLKRAGIN
                     TVEELCNKTSDDMMKVRNLGRKSLEEVLAKLKELGLQLQPAEE"
     gene            1108..1644
                     /locus_tag="ACZPD3_07590"
     CDS             1108..1644
                     /locus_tag="ACZPD3_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HIR1415887.1"
                     /translation="MAKYRKLSRTSDQRKALLRSQVTSLLYHGKIVTTEAKAKEIRKI
                     AEGLVAMAVREKDNFETVTVTAKVARKDAEGKRVKEVVDGKKKTVYDEVQKEIKKDAP
                     SRLHARRQMMKVFYPVKEVPAKGAGRKKNTKDVDMVAKMFDEIAPKYADRNGGYTRIV
                     KIGPRKGDAAMEVLIELV"
     gene            1927..2814
                     /locus_tag="ACZPD3_07595"
     CDS             1927..2814
                     /locus_tag="ACZPD3_07595"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C/glycerophosphodiester phosphodiesterase family protein"
                     /protein_id="HIR1415888.1"
                     /translation="MRQNTKKKRSGFGYFIRMFLLLVELLAVTLFLWGNDAYSISATT
                     PEFKWENYKTIAHALGGIGDKTYLNSKESFLAGYQMGCRLFEVDLVKTSDNVWVCRHS
                     WYQSLGQWEGDEKKVLSSEEFLSRPIYGKYTPITFEDLLVLLSDYPDAFVMLDSKQYS
                     LRNYQKTVEDYADYIELAEAAGVPDVMRQVIPEIYNQAMFAGTALLYDFPGYIYSLWQ
                     EYSIKELTEIAAFCREKNIQAATVYYKYWSEDVQKIFDKKGIRLYIYTVNDLKEAQYY
                     MQEGAAGVCSDYLQDTMFN"
BASE COUNT         1040 a          518 c          750 g          822 t
ORIGIN      
        1 aaccctatag tgtttgattt taataaacct aagatcgaga ttaccgaatt atctgaagat
       61 aaaaaatttg gcagattcgt agttgagcct ctggaaagag gatatggaac tacacttgga
      121 aattctttaa gaagaattat gctgtcttcc ctgccgggtg cagctgtaag ccaggttaaa
      181 attgatggtg tgcttcacga attcagctcc attccgggcg taaaagaaga cgtgagcgat
      241 attatcatga atctgaagag ccttgctatt aagaaccaca gcacagacaa tgagccgaaa
      301 acagcataca tcgagtgtga aggaaaaggt gttgtaactg cagctgatat ccaggctgat
      361 caggatatcg agatcatgaa tccggatcag gtgattgcta ctttaaacgg tggcaaggac
      421 tgcagacttg ctatggagct tactattaca aaaggccgcg gatatatcag tgctgataaa
      481 ggtaaatctg atgatatgcc gatcggtgta cttgcagttg atgctatcta tacaccggta
      541 gacagagtca acatgactgt agagaataca cgtgttggtc aggtaaccga ttatgataag
      601 ctgacactgg atgtgtatac aaacggtaca cttgatccgg atgaggcggt aagccttgca
      661 gcaaaagtat taagcgagca cttgagcctt ttcattgacc tttccgagaa tgcaaagacc
      721 gctgaggtta tgatcgagaa agaagacaat gagaaagaaa aagtcctgga aatgagcatt
      781 gatgaacttg agttatctgt tcgttcctat aactgcttga agagagccgg cattaataca
      841 gttgaagaat tatgtaacaa gacttctgat gatatgatga aggttcgtaa ccttggacgt
      901 aaatcattag aagaagttct tgcgaaactg aaagagctgg gcttacagct tcagcctgca
      961 gaagagtaag aactgatcag gttattggtt ctctaaggga acaataacca gttagcaaag
     1021 agatattata cggtgggaat gcctaagcag tcggacagta agaccgaaca agcgtgcccg
     1081 gctgtcccac agatggagga aaattaaatg gcaaaatata gaaaattaag cagaacttct
     1141 gaccagagaa aagcattact gagaagccag gtaacaagcc ttttatatca tggaaaaatt
     1201 gttactacag aagctaaagc aaaagaaatc cgtaaaatcg cagaaggcct tgtagctatg
     1261 gctgttcgtg aaaaagataa cttcgaaact gttactgtaa ctgctaaagt tgctcgtaaa
     1321 gatgctgaag gcaagagagt aaaagaagtt gttgacggaa agaagaaaac tgtatacgat
     1381 gaagtacaga aagagatcaa gaaagatgct ccttccagac ttcacgctcg tcgtcagatg
     1441 atgaaagttt tctacccggt taaagaagta cctgcaaagg gtgccggaag aaagaaaaac
     1501 actaaagacg tagatatggt tgctaagatg ttcgatgaga tcgctccaaa atacgctgac
     1561 cgtaacggtg gttacacaag aatcgttaag atcggaccgc gtaaaggcga tgctgcaatg
     1621 gaagtactga tcgaattagt ataatccagc cagattttcg attttatatt tgtccagacg
     1681 atgataagac tgcatatgta tggtgttcat gccgtatata tgcagttttt ttgttatgct
     1741 ataggaaatc acttcctgta acataaccgt ttattaccct tgattatttg agccagatag
     1801 cgtaaaatct tagagtatat aaaaaccata ttgatgaaga agtgttaaat aagacattgg
     1861 actgttacaa aatacgagag caaaagataa gcgaggaata caaatgaccg gcaggagatt
     1921 gaaaaattga ggcagaatac gaaaaagaaa cgatcaggat ttggttattt catcaggatg
     1981 tttttgttgt tggtggaatt actggctgtt acactgtttc tgtggggaaa tgatgcttat
     2041 tccatatctg ccaccacacc tgaatttaag tgggaaaatt ataaaacgat cgcacatgca
     2101 ctgggaggga ttggtgataa aacctatctg aattcaaaag agagttttct ggcaggctat
     2161 cagatggggt gcaggttgtt tgaagttgat cttgtgaaga catctgacaa tgtatgggtg
     2221 tgccgccata gctggtatca gtctcttgga caatgggaag gcgatgaaaa gaaagttctg
     2281 tcctcagagg aattcttatc ccggcctatt tatggaaaat atacgcctat aacatttgaa
     2341 gatctgctgg ttctgctgag tgattatccg gacgcttttg tgatgctgga ttccaaacag
     2401 tattctctcc gtaattatca gaaaacagtg gaagattatg cagactatat tgaactggca
     2461 gaggctgccg gtgtcccgga tgtgatgcgg caggtcatac cggaaattta taaccaggca
     2521 atgtttgccg gaacagcact gctgtacgat tttccaggat atatttattc tttatggcag
     2581 gaatactcta ttaaagaact gactgaaatt gcggcatttt gcagagaaaa gaatattcag
     2641 gcagctacag tctactataa atactggtca gaggatgtgc agaagatttt tgacaaaaaa
     2701 ggaatcaggt tatatattta tactgtgaat gacctgaagg aagcacaata ttatatgcaa
     2761 gagggtgcgg caggtgtatg ctcagactat ttgcaggata ctatgtttaa ctgagttgtt
     2821 ctgccggcat tatgaaatga tcatagagga ttattatagc tatagctgtt ccggaatgaa
     2881 taagggtaaa gatatcagga gcaaaaagaa ttttaacagg atatagcaag aattctccct
     2941 cctctatagg tggtagatga attgcaaata agaaaatgaa cggcagtggc gggcggagag
     3001 aaatcggtat ctcccactga catgaggcaa accacagtca gtatgttgtt gacacgtcag
     3061 actctgtgaa attaccgaca gtgagttgaa acaaataagc ggcaataaca aagattatat
     3121 ttataaagga
//
LOCUS       DBMDXG010000266         3123 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_6977_length_3123_cov_1.481469, whole genome shotgun sequence.
ACCESSION   DBMDXG010000266 DBMDXG010000000
VERSION     DBMDXG010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6977_length_3123_cov_1.481469"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="ACZPD3_07600"
     CDS             <1..778
                     /locus_tag="ACZPD3_07600"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR1415889.1"
                     /translation="FEENDRLNITEYTQIAMLTAEIAILRAVEEAGIRSQVNAGLSLG
                     EYGALVASGVMKEEDAFTVVRKRGIFMQEAYPTGGAMSAVLGTDAELIEKICNETQGI
                     VSIANYNCPGQIVITGEETAVAAAGEALKAAGARRVIPLKVSGPFHCELLKGAGEKLG
                     QELEKVEIQSFTVPYVTNVTAQYVTGPEQVKELLVSQVSSSVRWQQCVEQMIDDGVDT
                     FIEIGPGKTLTGFLKKINRNVKALHVEKTEDLDEVRKECL"
     gene            780..1520
                     /gene="fabG"
                     /locus_tag="ACZPD3_07605"
     CDS             780..1520
                     /gene="fabG"
                     /locus_tag="ACZPD3_07605"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR1415890.1"
                     /translation="MLKNKIALITGAGRGIGRAIAIALAKEGAEVVINYNGSEERAKE
                     VKQTIEENGGKASIYKCNVSDFVACEAMIKDIVKEYGHLDILVNNAGITKDGLIMKMK
                     EEDFDSVLNVNLKGTFNTIRHSARQMLKQRSGKIINISSVSGILGNVGQANYAASKAG
                     VIGLTKTMARELGSRGITVNAIAPGFVDTEMTEVLSEEIRENACKQIILGRFGKPEDI
                     ANTAVFLASDKADYITGQVISVDGGMNV"
     gene            1535..2770
                     /gene="fabF"
                     /locus_tag="ACZPD3_07610"
     CDS             1535..2770
                     /gene="fabF"
                     /locus_tag="ACZPD3_07610"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR1415891.1"
                     /translation="MRRVVITGMGAITPVGLSVEEFWNSVKEGKTNFAEVTRFDSTNY
                     RAHMAAEINNFNPKDYMDFKSAKRMELFSQYAVAAAKEAIEQSGLDMTKEDPFRVGCS
                     VGSGIGSMQVVEKSCEILNTKGPGRLNPLMIPLLISNMASGNVSIQFGLRGKNINVVT
                     ACATGTHSIGEAYRTIQCSDADVMVAGGTEAAITPTGFGGFAALTALTSSTDPERCSI
                     PFDKERSGFVMGEGAGVVVLEELEHAKARGAKILGEVVGYGATGDAYHITSPAEDGSG
                     AAKAMEWAVKEAGISTDDVWYVNAHGTSTHHNDLFETIAIKKTFGEHAKEMKINSTKS
                     ITGHLLGAAGAVEVITCVKELQEGLIHQTVGYKVPDEQCDLDYVGNGNVKMDIKYALT
                     NSLGFGGHNASLLIKKYED"
     gene            2858..>3123
                     /locus_tag="ACZPD3_07615"
     CDS             2858..>3123
                     /locus_tag="ACZPD3_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR1415892.1"
                     /translation="MELANILELIHAVSDSDLTEFNLQDDTLNISMSKEKTIVQQMAV
                     NTDPADAQQYQPVVQQAVHVESVNAVNDEVQTGSVVKSPLVGTY"
BASE COUNT         1030 a          553 c          792 g          748 t
ORIGIN      
        1 ctttgaagaa aatgacagat taaatattac agaatataca cagattgcca tgctgacagc
       61 agaaattgca atcttaagag ctgtagagga agccggaatc aggtctcagg taaatgctgg
      121 actgagcctt ggagaatacg gagcactggt ggcctccggt gttatgaagg aagaggatgc
      181 atttactgta gtgcgaaaaa gaggaatttt tatgcaggag gcatatccta caggtggtgc
      241 aatgtccgct gttcttggaa cagatgcaga gctgattgaa aaaatctgca atgagacaca
      301 gggaattgta tccattgcaa attataactg tccgggacag attgtgatca caggcgagga
      361 gacagcagtt gcagcagcag gggaggcatt aaaggcagca ggcgccagac gtgtgattcc
      421 tcttaaagtc agtggcccat ttcactgtga gcttctcaaa ggcgcagggg aaaaactggg
      481 acaggaactt gagaaggtag aaatccagtc atttacagta ccttatgtga caaatgtaac
      541 tgcacagtat gtgacaggac cggagcaggt aaaggaattg cttgtcagcc aggtttcatc
      601 ctctgtgcgc tggcagcagt gtgtagaaca gatgattgat gatggtgtag atacttttat
      661 tgagattgga ccgggaaaaa ctctgacagg atttctcaaa aagatcaaca gaaatgtaaa
      721 agcattacat gttgagaaaa cagaagatct ggatgaagtg agaaaggagt gtctgtaaaa
      781 tgctgaaaaa taaaattgcc ctgattacag gtgcaggacg tggaatcgga cgagccattg
      841 ctattgctct tgcaaaggaa ggtgcagagg tggttatcaa ttacaatggt tctgaagaga
      901 gagcgaagga agtaaaacag accatcgaag aaaacggtgg aaaagcttcc atatataaat
      961 gcaatgtcag tgatttcgta gcctgtgaag caatgatcaa agatatagtt aaagaatacg
     1021 gacaccttga tattctggtt aataatgcag gaattaccaa agatggtctg atcatgaaaa
     1081 tgaaagaaga ggatttcgat tctgtactta atgtaaacct gaaaggtaca ttcaatacaa
     1141 tccgtcacag tgcccgtcag atgctgaaac agagaagtgg aaagatcatc aatatttctt
     1201 ctgtttccgg aatcctcgga aatgtgggcc aggcaaatta tgctgcatcc aaagcaggtg
     1261 tgatcggtct gacaaaaaca atggcaagag agctgggcag ccggggaatt acggtaaatg
     1321 ccattgcacc cggatttgta gacacagaga tgacagaagt actctcagaa gaaatcaggg
     1381 aaaatgcatg taagcagatt atcctgggac gtttcggaaa gcctgaggat attgcaaata
     1441 cagcagtatt tctggcatca gacaaagcag actatattac aggacaggtc atcagcgtag
     1501 atggcggtat gaacgtataa ataaggagaa caaaatgaga agagttgtaa ttacaggtat
     1561 gggagcaatc actccggtag gccttagtgt agaagaattt tggaacagcg taaaagaagg
     1621 aaaaacaaac tttgcagaag taacacgttt tgattccaca aattacagag cgcatatggc
     1681 agcagagatc aacaatttta atccaaagga ttatatggat ttcaaatctg caaaacgtat
     1741 ggagcttttc agccagtatg cggtagcagc agcaaaagaa gctattgagc agtccggact
     1801 ggatatgaca aaagaagatc ccttccgtgt aggctgtagc gtgggaagcg gtatcggaag
     1861 tatgcaggtg gtagagaaat cctgcgaaat tctcaacaca aagggaccag gaagactgaa
     1921 tcctctgatg attcctcttc tgatctccaa tatggcatcc ggaaatgttt ccattcagtt
     1981 tggactccgc ggaaagaaca ttaatgttgt aactgcctgt gcaacaggaa cccacagcat
     2041 tggagaagca taccgtacta ttcagtgctc tgatgcagat gttatggttg caggtggaac
     2101 ggaagcagct atcacaccaa caggctttgg tggttttgcg gcactgacag cattgacctc
     2161 atccacagat cctgagagat gttccatccc ttttgacaaa gaaagaagcg gttttgtaat
     2221 gggtgagggt gccggcgtgg tagttctgga agaactggag catgccaaag cacgtggagc
     2281 gaagatcctt ggcgaggttg tcggatatgg tgcaacagga gatgcttatc acattacatc
     2341 ccctgcagag gacggttccg gtgctgccaa agctatggaa tgggctgtaa aagaagcagg
     2401 aatttccaca gatgatgtat ggtatgtaaa cgcacatggc acaagcacac atcataatga
     2461 cttatttgaa acaattgcca ttaagaaaac atttggtgaa cacgcaaaag aaatgaaaat
     2521 aaattctaca aaatctatta caggacatct tctgggagct gccggtgcag tggaagtgat
     2581 cacctgtgta aaagaacttc aggaaggtct gatccatcag actgttggtt ataaggtgcc
     2641 ggatgaacag tgtgacctgg attatgtagg aaacggtaat gtaaagatgg atatcaaata
     2701 tgcacttacc aattctcttg gctttggcgg acataacgca tccttactta ttaaaaagta
     2761 cgaagactga tcacgtagat aataaatggt atggtgattt tgaaaacaat atacgacagt
     2821 gatcttataa tatagctatt tcagcaggag gaaataaatg gaactggcaa atatattaga
     2881 actgatccac gcagtcagcg attcagatct cacagaattt aacctgcagg acgatacctt
     2941 aaatatttcc atgtcaaaag agaaaacaat tgtacagcag atggcagtca atactgatcc
     3001 ggcagatgct cagcagtatc agccggtagt gcagcaggct gtacatgtag aatctgtcaa
     3061 tgctgttaac gatgaagtac agacaggaag tgtggtaaag tctcctcttg taggtaccta
     3121 tta
//
LOCUS       DBMDXG010000267         3108 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7019_length_3108_cov_0.843365, whole genome shotgun sequence.
ACCESSION   DBMDXG010000267 DBMDXG010000000
VERSION     DBMDXG010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_3108_cov_0.843365"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            125..241
                     /locus_tag="ACZPD3_07620"
     CDS             125..241
                     /locus_tag="ACZPD3_07620"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415893.1"
                     /translation="MRNIREWLKISSKCDAHLAESFFQTHSEENIAYFAKKV"
     gene            380..1531
                     /gene="ftsZ"
                     /locus_tag="ACZPD3_07625"
     CDS             380..1531
                     /gene="ftsZ"
                     /locus_tag="ACZPD3_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR1415894.1"
                     /translation="MSNEAESSAKIIVIGVGGAGNNAVNRMVEEAIGGVEFVGVNTDK
                     QALTLCKAPTVLQIGEKITKGLGAGAQPEVGQKAAEESIEEVKQIIEGADMVFVTCGM
                     GGGTGTGAAPVIAAAAKEMGILTVGVVTKPFRFEAKTRMNNALAGIENLKKAVDTLIV
                     IPNDKLLEIVDRRTTMPEALRKADEVLQQAVQGITDLINLPALINLDFADVQTVMTDK
                     GIAHIGIGEARGDDKAMEAVQQAVSSPLLETTIKGATHVIINISGDISLMDANDAASY
                     VQELTGEDANIIFGAMYDDSVADYARITVIATGLSDNAQASNPFGNKASNSVFANKRP
                     ASTTASAGVNLNMPSFSMPTMNATPFTAKTPSSTVQKKDIQIPDFLRNR"
     gene            complement(1669..1989)
                     /locus_tag="ACZPD3_07630"
     CDS             complement(1669..1989)
                     /locus_tag="ACZPD3_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR1415895.1"
                     /translation="MTILKCSATTCAYNEDMLCSRGEINVMGDSAKSSEETSCGSFRE
                     KSQGAMNSAKNSCGCETIQIDCKAHNCTYNENCKCTAAAIDVAGSGAKVCSETKCDTF
                     ECQC"
     gene            2449..2943
                     /gene="infC"
                     /locus_tag="ACZPD3_07635"
     CDS             2449..2943
                     /gene="infC"
                     /locus_tag="ACZPD3_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR1415896.1"
                     /translation="MINEQIRDKEVRLIGPDGEQLGIMSAREAMAKAQEAELDLVKIA
                     PQAKPPVCKIIDYGKYRYELARKEKEAKKKQKTIDVKEVRLSPNIDTNDLNTKAGAAR
                     KFLTKGDRVKVTLRFRGREMAHMSSSKHVLDDFADMLKDIAVVEKAPKVEGRSMTMFL
                     AQKH"
     gene            3032..>3108
                     /locus_tag="ACZPD3_07640"
     CDS             3032..>3108
                     /locus_tag="ACZPD3_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR1415897.1"
                     /translation="MPKIKTCRAAAKRFKKTGTGKLVRN"
BASE COUNT          973 a          585 c          747 g          803 t
ORIGIN      
        1 aaagatggaa attatgtagg ccccaatccc aatgaaacca gtgaagatgg aagcggagag
       61 gatcttgaag attacagtgg ggactacagc gaagactata atgacagcgg ttatgacagt
      121 tacgatgagg aatattagag agtggctgaa aatctccagt aaatgtgatg cacatcttgc
      181 agaaagcttt tttcaaacac actctgagga aaatatcgcg tatttcgcga aaaaagtata
      241 aaaaaacaaa attagtggtt gataaattca caaaaaaaat atatgatata gctatatgca
      301 gcttggtata tatatttgct gaaattaaag ataaaggata taaggtagaa ataaaggagg
      361 aagtacattg ttagagataa tgtcaaatga ggcggaatcc tctgctaaga ttattgtaat
      421 cggagtagga ggtgccggaa ataacgcagt taacagaatg gttgaagaag ccatcggcgg
      481 ggtagagttt gtaggagtga atacagataa gcaggctctg actctgtgta aagctccaac
      541 tgtacttcag atcggtgaga aaattaccaa aggtcttggt gcaggagcac agcctgaggt
      601 tggacagaaa gcagcagaag agagcattga ggaagtaaaa cagatcatcg aaggtgctga
      661 catggtattc gttacctgcg gtatgggcgg tggaactggt acaggtgctg caccggttat
      721 cgcagcagca gctaaggaaa tggggatcct cactgtaggt gttgtgacca aacctttccg
      781 ttttgaagct aagacacgta tgaacaacgc acttgcaggt attgagaacc tgaagaaagc
      841 tgttgataca ttaattgtaa ttccgaatga caaattactg gagattgtag atcgccgtac
      901 aactatgcca gaagcacttc gtaaagcaga cgaggttctg cagcaggcag ttcagggcat
      961 cacagatctg atcaatctgc cggctcttat caaccttgac tttgctgatg ttcagactgt
     1021 tatgacagat aaaggtattg cacacattgg tatcggtgag gcaagaggcg atgacaaggc
     1081 tatggaggct gtacagcagg ctgtatcctc tccgcttctt gagactacca tcaagggtgc
     1141 tacacacgtt atcatcaaca tttccggtga tatttccctt atggatgcaa atgatgctgc
     1201 aagctatgta caggagctta caggtgaaga tgcaaatatc atctttggtg ctatgtatga
     1261 tgactctgtt gctgattatg cgagaatcac tgttattgct acaggtcttt ctgataatgc
     1321 acaggcatcc aatccatttg gaaataaggc aagcaactct gtatttgcca ataagagacc
     1381 tgcttccact acagcgtctg ctggcgtgaa tctgaacatg ccgtctttca gcatgccgac
     1441 tatgaatgct acaccattca cagcgaagac accatccagc actgttcaga agaaggatat
     1501 ccagatcccg gatttcttaa gaaacagata atgaatggaa attaaaaggc cctcgcacat
     1561 ttgtgcgggg gctgtttttg aatttgcggt tattttctgg aatctgtttg aaaaattttt
     1621 gcagaaagcc tttttcagac acgttctaga gcctgtttca aacacgctct agcactggca
     1681 ttcaaaggta tcgcattttg tttcggaaca tacttttgca ccgcttccgg ctacatcaat
     1741 agcagctgcg gtacatttac agttttcgtt ataggtacag ttgtgggctt tgcagtcaat
     1801 ctggatggtc tcacatccac agctgttttt ggcactgttc atggcaccct ggcttttttc
     1861 ccggaagctt ccgcaggatg tttcttcaga actttttgca ctgtcaccca tgacgttgat
     1921 ttcccctctg gaacaaagca tatcttcatt gtaagcacag gttgttgcgg aacattttaa
     1981 aattgtcatg ttttttacct cctgattttg tatgcttgat tttgcaaaca tttgcagttg
     2041 ttcagcagac cgtattttat gaggacacta aactgctgca gcagtcttag tttctcctgt
     2101 gccggcaaaa atatgcacaa atttataaga aaaaataaaa aaacatcttg actttcataa
     2161 taaggcatga tatcatacga cagtacgtga tgaaaagaaa gcagagttca ctctcacctc
     2221 agcgcatact tagatgcggc tcgggttcat attgcacagc agcgcgtgac ttagttcgcg
     2281 ggtgtaatag gagaagtgtg gattctgtcc gcactttttt tatatttgcg gcgtcaggta
     2341 taaggcagga atttcttcct gcgaagggga aagagagtcc agaagttaga aactggatca
     2401 gaacagatca tctatcaggt atggaggtgc acgacaatta gcaatttaat gattaacgaa
     2461 caaatcagag acaaagaggt acgcttaatc ggaccggacg gagagcagct cggaattatg
     2521 tccgcaagag aagcaatggc caaagctcag gaggcagagc ttgatttagt aaagattgcc
     2581 ccacaggcaa agccgccggt atgtaagatc attgattatg gtaaatatcg ctacgaactt
     2641 gccagaaaag agaaagaagc caagaaaaag cagaagacca tcgatgtcaa agaagtgcgt
     2701 ctttcaccaa atattgatac caatgacctg aatacaaagg ctggggcagc gcgcaaattc
     2761 cttaccaagg gcgacagagt gaaggttact cttcgtttcc gcggaagaga gatggcacac
     2821 atgtcaagca gcaagcacgt tctggatgat tttgccgata tgctgaagga cattgctgta
     2881 gttgagaagg cacctaaggt agaaggcaga agcatgacaa tgtttttagc acagaaacac
     2941 taggatgttg cattcatttg gagagacacc ctaagggtgg caggctggat gagggacatt
     3001 aaatagatac gatattaagg aggaaattac aatgccaaaa attaaaactt gtagagcagc
     3061 agcaaagcgc ttcaaaaaaa caggaacagg aaaattagta agaaataa
//
LOCUS       DBMDXG010000268         3107 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7022_length_3107_cov_1.424484, whole genome shotgun sequence.
ACCESSION   DBMDXG010000268 DBMDXG010000000
VERSION     DBMDXG010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7022_length_3107_cov_1.424484"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="ACZPD3_07645"
     CDS             <1..181
                     /locus_tag="ACZPD3_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415898.1"
                     /translation="NKSIPSGEVWGGVPAHYIMKTKDYADKCKENTPIYDLENYRKNF
                     REEVNLICDRMEHSM"
     gene            395..1297
                     /locus_tag="ACZPD3_07650"
     CDS             395..1297
                     /locus_tag="ACZPD3_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR1415899.1"
                     /translation="MKTTLLIMEAGIGSRFGTGIKQLEPLDDAGHINMDYSIRDAIEA
                     GFNHVVFIIRKDIEKEFKEVIGDRIASICSSHNVTVDYAFQDINDIPGTLPEGRTKPW
                     GTGQAVLAAKKAINTPFIVINADDYYGKEGFKAVHEYLVNGGKSCMAGFVLKNTLSDN
                     GGVTRGICKMDEQNNLTEVVETKNIVKTATGAEADGKVIDVNSLVSMNMWGLTPEFLD
                     VLEEGFEEFFEKEVPGNPLKAEYLIPIFIGELLEQGKMSVKVLKTNDTWYGMTYHEDV
                     AAVKDSFKKMLADGVYKAELFSDL"
     gene            1304..1915
                     /locus_tag="ACZPD3_07655"
     CDS             1304..1915
                     /locus_tag="ACZPD3_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR1415900.1"
                     /translation="MKETIDLLGKILTNILTALYEPFGFSLLLSFLAMFFYLYAYEPT
                     AAGKGWKSAIVTWYQKFKESVFFRKLFFLAFVTSLILFQTLLNRQLWMNPLSDVMGGW
                     GIWETVNGEQKLTTECIENVIMMVPFSAVVMWTFEEKMEKGWKKIVWQSGKIAFIFSI
                     SIEMLQLLLRLGTFQLSDIFYNTVGGVLGGLMYCAVMKVRKRL"
     gene            2212..2511
                     /locus_tag="ACZPD3_07660"
     CDS             2212..2511
                     /locus_tag="ACZPD3_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415901.1"
                     /translation="MEGNMQEYLTEKKAREKRLAVYTEKIQETVLNKSAETIRLLVEE
                     RHNQKMTQQELSDITGIQPANLARFESGGRIPTLIVLEKYANALGKHIEIKICDD"
     gene            2702..>3107
                     /locus_tag="ACZPD3_07665"
     CDS             2702..>3107
                     /locus_tag="ACZPD3_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415902.1"
                     /translation="MDVNCTVITGYHGTSSNYTESIEKHGLDPDKTHTRPDHWLGQGV
                     YFFEDSDLAKWWACDISGKKYNQGSYPVIYQAQIQAPREEVLDLDNYKEYDLFLNRIL
                     EMQNEIECDAKNRMPVFSPEQQRAVYFDYYKKM"
BASE COUNT          999 a          513 c          789 g          806 t
ORIGIN      
        1 gaataaaagt ataccctccg gagaagtgtg ggggggggta ccagcccatt acatcatgaa
       61 aacaaaagac tatgcagaca agtgtaaaga aaatactcct atctatgatt tagaaaacta
      121 tcggaagaat tttcgtgagg aggttaattt gatctgcgat aggatggaac atagcatgtg
      181 aaataaggaa tagggtccga cagcgaggat agtctaggta attaggagaa tattattggg
      241 ttccattggg gtcagacccc aagaagaaat aatttttttc taaagaaact ataaacgaaa
      301 atgagagcat aggctactgg cgctgcttta ataatcacaa aaatctttgt ggaaggcacg
      361 ttgagtaggc ttcaaggcat gtaaaggaga gaggatgaaa acaacattac ttatcatgga
      421 agccggaatc ggcagccgct tcggaacagg aattaaacag ttggagccgt tggatgatgc
      481 tggacatatc aacatggact actcaatccg tgatgcgatt gaggcaggtt tcaaccatgt
      541 agtatttatc atccgcaagg atatcgagaa agagtttaaa gaggttatcg gtgatcgtat
      601 tgcctccatt tgctcttctc ataacgtaac agtagactac gctttccagg atattaatga
      661 tattccggga actctgccgg aaggtcgtac aaaaccttgg ggaaccggac aggcagtgct
      721 tgcggcgaag aaggctatca atactccgtt cattgtcatt aacgcagacg attactacgg
      781 caaagaaggc ttcaaggctg tccatgagta tttggtgaac ggcggtaagt cctgcatggc
      841 tggcttcgtg ctgaagaaca cgctgtctga taatggtggt gtgacccgtg gcatctgcaa
      901 gatggacgag cagaacaacc tgaccgaagt tgtggagaca aagaatattg tgaagaccgc
      961 aaccggagca gaagcggacg gaaaagtgat tgatgtaaat tctctggttt caatgaatat
     1021 gtggggatta acaccggagt ttttggatgt gttggaagaa ggcttcgaag agttctttga
     1081 gaaggaggta ccgggcaatc cgctgaaagc agagtatctg atccctatct tcatcggtga
     1141 actgctggag cagggaaaga tgtcagtaaa ggttctgaag accaacgata catggtatgg
     1201 tatgacatac catgaagatg tcgcagctgt aaaggatagc ttcaagaaga tgctggcgga
     1261 tggcgtgtac aaggctgaac tgttcagtga tctgtaaacg acgatgaaag aaacgattga
     1321 tttactcgga aagatcctca caaacatttt aactgctctt tatgagccgt ttggattctc
     1381 gctcttgctt tcttttcttg ctatgttttt ctacctgtat gcgtatgagc ctaccgcagc
     1441 aggaaaaggc tggaagagtg ccatagtgac atggtatcag aaattcaagg agagtgtgtt
     1501 ctttcggaaa ctattctttc tggcctttgt gacttcactt attctgttcc agaccctgtt
     1561 aaaccgtcag ctgtggatga atcctttatc cgatgtcatg ggtggctggg gcatctggga
     1621 gacagtgaat ggcgaacaga agctgaccac cgagtgtatc gagaacgtaa tcatgatggt
     1681 accgttttca gcagtagtga tgtggacatt tgaagaaaag atggagaaag gctggaaaaa
     1741 gatcgtgtgg caaagcggta agatagcatt tatcttttca ataagcattg aaatgttgca
     1801 gttattgctt cgcttgggta cattccagct atcagacatc ttctataaca cagtcggcgg
     1861 agtgctaggc ggattgatgt attgtgcggt gatgaaggta agaaagcgtc tgtaaaactg
     1921 gatgaaattg tgatatactg ggagcggtag ctccctacgc tgctcgtaat aatcacacac
     1981 ctcttcgtgg tgagcagcag gcaacgctga tataagcggc taagatgtga tgaattgata
     2041 aacataggaa gtggggactt ccagggtctg accctatgaa gtacaactaa aaaggataaa
     2101 gcagagctaa aagatctata aaagaagcat aaactctctt gccgagatat aacattgatg
     2161 atataatcct gaaaaataaa ggtaaataac tactggaaaa ggaaatggaa tatggaagga
     2221 aacatgcagg aatatctcac agaaaaaaaa gcccgtgaga aacggctggc agtttacaca
     2281 gaaaagatcc aggaaacagt actaaacaaa tcagcagaga caatccgctt gttggtagaa
     2341 gaaagacata accagaaaat gactcagcag gagctttcag atattacggg gatacagccg
     2401 gcaaacctgg cgcgttttga aagcggtggc aggataccga cactgattgt attggagaaa
     2461 tatgcgaatg ccttggggaa acatattgaa ataaagatct gtgacgactg acggattttt
     2521 atttcaattt aatcatgaat gataatgtat ataaagacac taaacgagaa gatatggata
     2581 tcacaagaaa attaagactc tgaaaggttg tctcgtttaa gttttgactt gtagggtagt
     2641 ataaagtatg ctatgattca ggtagtaaat cgttgggcga tgatatattg ggataaataa
     2701 tatggatgtg aattgcacag taataacagg ttatcatgga acaagttcta attatacaga
     2761 aagcatcgaa aaacatggtc ttgatccgga taaaacacat acgagacctg atcattggct
     2821 tggtcagggc gtgtattttt ttgaagattc tgatcttgca aaatggtggg catgtgatat
     2881 atcaggtaag aaatataatc aggggagcta tccggtcata tatcaggctc agattcaggc
     2941 accaagagaa gaagttttgg atttggataa ttataaagaa tatgatttgt ttctgaatcg
     3001 tattttggaa atgcagaatg aaattgaatg tgatgccaaa aaccgaatgc ctgtgttcag
     3061 tcctgaacag cagagagctg tttattttga ttactataag aagatgt
//
LOCUS       DBMDXG010000269         3091 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7060_length_3091_cov_1.714381, whole genome shotgun sequence.
ACCESSION   DBMDXG010000269 DBMDXG010000000
VERSION     DBMDXG010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7060_length_3091_cov_1.714381"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            12..416
                     /locus_tag="ACZPD3_07670"
     CDS             12..416
                     /locus_tag="ACZPD3_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016280722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR1415903.1"
                     /translation="MRGAYPAARATRELYALISAVKKVAIDEGCRDEIKSDLVDRVIL
                     KELKTVKEQLDTCPESREDLKAEYQARYDVIAKYAPKQMDAAEVKAYLEEKFADVIAT
                     KNKGQIMKAVMADMKGKADGKVINQVVAELCK"
     gene            607..1455
                     /locus_tag="ACZPD3_07675"
     CDS             607..1455
                     /locus_tag="ACZPD3_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1415904.1"
                     /translation="MEFQHELIIPNEGFPFKVFLFEGGNGNYVREKHWHTSVEIFAVM
                     EGRLDFFVNKDEYPLKAGEQIIINSNETHSIHAVEKNKTVVLQIPLKQFENYFTAQRY
                     IRFRGQEELVDKKLASLLRKLYHVYFERKIGYEFRTISIFYEIMYILVKDYRVTETRE
                     KDIRHSRRLDALSKITTYMREHYREELKLSDVAATFGYSDAYLSRMFQKYAKINYKTY
                     LQDIRMAYAYRDLLNTDHTISQIALDNGFCSSRGFSGEFQKRYGVLPSEMRKQINEKG
                     QKNAID"
     gene            1550..2167
                     /locus_tag="ACZPD3_07680"
     CDS             1550..2167
                     /locus_tag="ACZPD3_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HIR1415905.1"
                     /translation="MKIQNVTDASFRKYGKVLEGYDFSALLKEMKHTPVPDDVVYVPS
                     VEELEALDVAKALQNKGFGGIPIEIGYCNGHNKKLNAVEYHRSSEINVAVTDLVLLIG
                     SQQDITDDFTYDTSKIEAFLVPAGTGIEVYATTLHYAPCNVQDGGFQCVVVLPAGTNT
                     DLTFETAKTGEDSLLTAKNKWLIAHEDAAIEGAVNGLRGENITID"
     gene            2261..>3091
                     /locus_tag="ACZPD3_07685"
     CDS             2261..>3091
                     /locus_tag="ACZPD3_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR1415906.1"
                     /translation="MNNIPVVKLGLIAVSRDCFPIQLSEKRRAAIKETYKGELYECPV
                     TVENEKDMEKALEDVNKAECNALVVFLGNFGPETPETLIAERFDGPCMYVAAAEGDGD
                     MINGRGDAYCGMLNCSYNLKMRHLKAYIPEYPVGTADDIAKMIADFVPVARAVIGVSN
                     LKIITFGPRPQDFFACNAPIKGLYELGVEIEENSELDLLVSFKEHENDPRIPEVCADM
                     AKEMGEGKYYADLNVKMAQFELTLLDWAEAHKGARKYVAFADKCWPAFPSQFGFEPCY
                     V"
BASE COUNT          971 a          535 c          772 g          813 t
ORIGIN      
        1 cagccgtaca gctgcgcggt gcgtaccctg cggcacgcgc cacccgcgag ctctacgcac
       61 tgatctctgc ggtaaagaag gtagcgatcg atgagggatg ccgtgatgag attaagagtg
      121 atctggtgga cagagttatt ctgaaagagt tgaaaactgt gaaagaacag ctggatacct
      181 gcccggagag ccgcgaagat ctgaaggctg aatatcaggc aagatatgat gtgatcgcca
      241 aatatgcgcc aaaacagatg gatgcagcag aggtaaaagc atatctggaa gaaaaatttg
      301 cagatgtgat tgcgacgaaa aataagggtc agatcatgaa ggctgttatg gctgatatga
      361 agggaaaagc agacggaaag gtgattaatc aggttgtggc agagctctgc aaataatgtg
      421 ttatcttaac cgaatcaagt aagtcccctg cttcaattgc gaaaagcaat aagcagtggg
      481 tgggactatg tgaagaatag gatcatccag cagatttggt atgcagaaag ctggatagtt
      541 atcaaaatag ccgcagtatg ctggaatgtc cggatactgc ggtattttat taggaggtga
      601 atatatatgg agtttcagca tgaactgatc attcctaatg agggatttcc gtttaaggtt
      661 tttctttttg aaggaggaaa tggcaattat gtgcgtgaga agcactggca tacgtctgta
      721 gagatttttg ctgtgatgga ggggcgcctg gatttttttg taaataagga tgaatatccg
      781 ctgaaagctg gagagcagat aatcattaat tccaatgaga cccactctat ccatgctgtg
      841 gagaaaaata aaacagtagt tctgcagatt cctctgaaac agtttgagaa ttattttact
      901 gcccagagat atatccgttt tcgggggcag gaggagctgg tggataagaa actggcttct
      961 ttgcttcgta aattatatca tgtgtatttc gagcggaaaa ttggatatga gttccggaca
     1021 atttctattt tttacgagat tatgtatatc ctggtgaagg attaccgtgt gacagagacc
     1081 agggagaagg atatccgaca cagccgccgt ctggatgcat tgtcaaaaat caccacatat
     1141 atgcgtgagc attacaggga ggaactgaag ctttctgatg tggcggctac ttttggatac
     1201 tcggatgcat atttgtcgcg aatgttccaa aaatatgcaa aaatcaacta taaaacttat
     1261 ctgcaggata tccgtatggc atatgcgtac agggatttgc tgaatacaga ccatacaatt
     1321 agtcagattg cactagataa tggattttgc agcagcaggg gtttttccgg ggaatttcag
     1381 aaaagatatg gggtacttcc aagtgagatg cgaaaacaga taaatgaaaa aggtcaaaaa
     1441 aacgctatag attagacaag aaaactggcg aaaagtaccc tttattctga tatattctaa
     1501 gcacagtcaa gaaaacgtaa tatctacaga atataaggag gtttttgaag tgaaaattca
     1561 gaatgtaaca gatgcttcct tccgtaaata tggaaaggtg cttgaagggt atgacttcag
     1621 tgctcttctc aaagaaatga aacatacacc ggttccggat gatgtggttt atgtaccatc
     1681 tgttgaggag ctggaagctc tggatgtagc aaaagcactg cagaataaag gatttggcgg
     1741 aatcccgatc gaaattggat actgcaacgg acataataag aaattaaatg cagtagaata
     1801 tcaccgcagt tcagagatca atgtagcggt aacagatctg gtgctgctta tcggaagtca
     1861 gcaggatatc acagatgatt ttacatatga tacttctaag atcgaagcat tcctggtacc
     1921 tgcaggaaca ggtattgaag tatatgcaac tacacttcat tatgcgccat gcaatgtaca
     1981 ggatggcggt ttccagtgtg tagtcgtact tccggcagga accaatacag atcttacatt
     2041 tgagacagct aagacaggcg aggacagcct gctgacagct aagaacaaat ggctcattgc
     2101 acatgaagat gcggcaatcg agggtgcagt aaacggactt cgtggagaga atatcaccat
     2161 tgactgagtt gctcggaatc tgtggcagta gagtatcaga atatagtaaa tgaataacaa
     2221 aatcatataa atacatatca aatatggagg aatttatatt atgaacaata taccagtagt
     2281 aaaacttgga ttaatcgcag taagccgtga ctgttttccg attcagcttt ctgaaaagag
     2341 acgtgcagca atcaaggaaa cttataaagg tgaactttat gaatgtcctg taactgttga
     2401 gaacgagaag gatatggaga aagctcttga agatgtaaac aaagcagagt gtaatgcgct
     2461 ggttgttttc cttggaaact tcggacctga aactcctgaa actctgatcg cagagagatt
     2521 tgacggacct tgtatgtatg tagcagcagc agaaggtgac ggtgatatga tcaacggcag
     2581 aggtgatgct tactgtggta tgcttaactg ctcctacaac ttaaaaatgc gtcatctgaa
     2641 agcatatatt ccggaatatc ctgttggaac agcagatgat attgcaaaga tgatcgctga
     2701 tttcgtaccg gttgcacgtg cagtcatcgg tgtcagcaat cttaagatca ttactttcgg
     2761 accacgtcca caggacttct tcgcatgcaa cgcacctatt aaaggcctct atgagctggg
     2821 tgttgagatt gaagagaact cagagcttga tcttcttgtt tctttcaaag agcatgaaaa
     2881 tgatcctcgt atcccggaag tttgtgcaga tatggcaaaa gagatgggcg aaggcaaata
     2941 ttatgctgac cttaatgtga aaatggcaca gtttgagctt acacttcttg actgggcaga
     3001 ggctcataag ggagcaagaa aatacgttgc atttgctgac aaatgttggc ctgcattccc
     3061 gtcacagttt ggatttgaac cttgctatgt a
//
LOCUS       DBMDXG010000270         3084 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7079_length_3084_cov_1.432115, whole genome shotgun sequence.
ACCESSION   DBMDXG010000270 DBMDXG010000000
VERSION     DBMDXG010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7079_length_3084_cov_1.432115"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="ACZPD3_07690"
     CDS             <1..685
                     /locus_tag="ACZPD3_07690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF025854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1415907.1"
                     /translation="NAYRFQLTYGEYSRQMEQKLQIQKQNYEELTEKVDEISRMRHDM
                     RHHLRTIMSYTQQGKYQEMMEYLQEYASVITEEEKRICYCRNMAVDAVIHFYAGELRE
                     KGIPFECDMMLPQNIGISDTDLCKIYGNLLENAVDAVKDLLPENKPYVKMLTKVKNKK
                     LLIEISNPYSNEIQRREKVFYSTKHEGFGIGTASVAEVVQRMGGYVVFNTEEGIFKVN
                     IFLPVKTVQ"
     gene            complement(730..1452)
                     /locus_tag="ACZPD3_07695"
     CDS             complement(730..1452)
                     /locus_tag="ACZPD3_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1415908.1"
                     /translation="MNIEILDDSKEDLRQVKSVISSYYESQNTSADIHLYSSAEAFFT
                     KYIPGFYDLIIMDIYMGSMTGMDAARKLREARDTTALIFISSSDSFAVESYDVQASYY
                     LLKPFDPEKLCRILSTIQFRQSQNSRYIELISDRTPVKIPVRSILYVDTYRNAVQVHT
                     TDAGIIRSYITFQKFEELLEGMKCFLSCYRGCIVNMDHIEKSTDEGFVMDNQELVTVR
                     KRGSNAIKKAYLEYLFSSDSDK"
     gene            1701..2513
                     /locus_tag="ACZPD3_07700"
     CDS             1701..2513
                     /locus_tag="ACZPD3_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415909.1"
                     /translation="MKNWKKIMAGLCAAAMVSSMVVPVWAAEETTEEAADDGDVADVS
                     EAMLGLWKDSAGDIYGFYKDNSFFGQWKDEEQDVLGVYALSSDGEYTALVMQFANDDG
                     TYDDENMVTYLVQANEEENLLELYDPDSLDLTATLEPYEADGDESDYNQTYQDMGDIL
                     TECYSGETEAGETFIYAANEDGTFCSVLVIDQDDNYVSFVGEGTFDEENGTVTITDEV
                     SEMALTFGVAVNDDDTLTLDMGDLGSATVEEATLAVAVQGLKYAVENGTEMN"
     gene            2724..>3084
                     /locus_tag="ACZPD3_07705"
     CDS             2724..>3084
                     /locus_tag="ACZPD3_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1415910.1"
                     /translation="MNILNIEHVSKVFGEKVVLDDVSYGVHQGDKIGIIGINGTGKST
                     ILKIIGGLEEPDEGQVITQNGLRITYLPQMPEFPQGASVLDYVAEGKWQKDWSTESEA
                     RNVLNKLVNTNRNTIATK"
BASE COUNT          955 a          497 c          789 g          843 t
ORIGIN      
        1 caatgcttac agatttcagc ttacctatgg agaatacagc cgtcagatgg aacagaaact
       61 gcagatacag aaacagaatt atgaggaact tacagaaaag gtggatgaga tcagcaggat
      121 gcgccatgat atgagacacc atctgcgcac catcatgtct tacacccagc agggaaaata
      181 tcaggagatg atggaatacc tgcaggagta tgcatccgtt ataacagaag aggaaaagcg
      241 aatctgttat tgcagaaaca tggccgtaga tgcagtgatc catttttatg caggagaact
      301 gagagagaaa gggattcctt ttgaatgtga tatgatgctg ccgcagaata ttggcatttc
      361 agatacagat ctgtgcaaaa tatatggaaa tcttctggaa aatgccgtag atgcagtaaa
      421 agatctgttg ccggaaaata agccttatgt aaagatgctt acaaaggtta agaacaagaa
      481 gcttctgata gagatttcaa atccatattc caatgaaatc cagcgaagag aaaaagtttt
      541 ttattcaaca aagcatgaag gctttggaat cggaacagcg tctgtagcag aagtggtaca
      601 acgaatgggc ggatatgtag tttttaatac agaagagggt atttttaaag taaatatctt
      661 tctgccggtg aaaacagtac agtaaataaa tagttactgc ccggagggga agaggcatat
      721 cggaaatact tatttatcag agtctgaaga gaataagtat tccagatatg ctttttttat
      781 tgcgttggag ccacgcttgc gtacagtaac aagctcctga ttgtccataa caaagccttc
      841 atctgtggat ttctcgatgt gatccatatt tacgatacag cctctgtaac aggagagaaa
      901 acatttcatt ccctccagaa gctcttcgaa tttctggaag gttatgtagg agcgtataat
      961 gcctgcatct gtggtatgta cctgaactgc attgcggtag gtatccacgt acaggatgct
     1021 cctgacaggg atctttacag gggtacggtc agagatcagt tctatatatc tggagttctg
     1081 tgactgtctg aactggattg ttgagagaat ccggcaaagc ttctccgggt caaagggctt
     1141 cagcagatag taggatgcct gtacatcata gctttccaca gcaaaggaat cactgcttga
     1201 aataaaaatc agagcagttg tatccctggc ttcgcggagc tttctggctg catccatacc
     1261 ggtcatactg cccatataaa tatccataat gatcaggtcg taaaatccgg gtatgtattt
     1321 agtaaaaaaa gcttctgcgc tggaatacaa atggatgtct gcggatgtat tctgggattc
     1381 ataataagaa gaaatgacag attttacctg acgcagatct tcctttgaat cgtcaaggat
     1441 ttcaatattc aaaaggagta cctcctaaat taacttaaac ataatttgta ttttagcgta
     1501 ttttcatgag ttattcaagt acggattcct tactgaaagc aagtaatacc ataagtattt
     1561 attcattttt ccatatttca tgaaaaatgt catttaatcc catgtatctt gaaaataaga
     1621 ctgaaatttt ttatagtaag aacatagaaa ttcagtttag aaatatgtac acacatttta
     1681 agaaaggatg gtaaaaaaat atgaaaaact ggaaaaaaat aatggcaggt ctttgtgctg
     1741 ctgcaatggt aagctctatg gttgttccgg tatgggcagc agaggagact acagaagaag
     1801 cagcagatga cggagatgtc gcggatgtat cagaggcaat gttaggactc tggaaggaca
     1861 gcgcaggaga tatctacgga ttctacaaag ataattcgtt ttttggccag tggaaggatg
     1921 aagagcagga tgttcttggt gtatatgctc tgagttcaga cggagaatat acagctctgg
     1981 taatgcagtt cgcaaatgac gacggcacgt atgatgatga aaatatggta acttatctgg
     2041 tgcaggcaaa tgaggaagag aatcttctgg aactttatga tccggacagt ctggacttga
     2101 ctgcaaccct ggagccttat gaggcagacg gtgatgagtc ggattacaat cagacttatc
     2161 aggatatggg tgatatcctg acagagtgtt attccggcga aacagaagcg ggtgagactt
     2221 ttatttatgc agctaatgag gatggaactt tctgctcagt acttgtgatc gatcaggatg
     2281 ataactatgt aagctttgtt ggcgaaggta cttttgatga agagaacggt actgtgacga
     2341 ttactgatga agtttctgag atggcactta cttttggcgt ggcagtgaat gatgatgata
     2401 cactgacact ggatatgggt gatcttggaa gcgctactgt agaggaagct acgctggcgg
     2461 ttgctgtgca gggactgaag tatgcagttg agaatggaac tgagatgaac taagcaattc
     2521 tttttcagga acaaaaatat gtgattttga ggatactcct cttcggatag atttattcgg
     2581 aggggagtgt tttttctgtg ccgggggttt tagaatggct gggaagatgg tataataagg
     2641 agcgaattct atagtttttg tggaagtgtt acaggtgagc agaaacagac aggaagcgaa
     2701 aattacgaga gaggtttgaa tttatgaata ttttgaatat agagcatgtt tccaaggtgt
     2761 ttggtgagaa ggtggtgctg gatgatgttt cctacggggt gcatcaggga gataagattg
     2821 ggatcatcgg gattaatggg accgggaagt ctacgattct gaagatcatc ggcggtctgg
     2881 aggagccgga tgaggggcag gtgatcactc aaaatggcct gagaatcacg tatcttccgc
     2941 agatgccgga gtttccgcag ggagcttctg tcctggatta tgtggcagaa ggaaagtggc
     3001 agaaggactg gagtactgag agcgaggcaa ggaatgttct gaataagctg gttaatacta
     3061 acaggaatac gattgccacc aaga
//
LOCUS       DBMDXG010000271         3078 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7097_length_3078_cov_1.810212, whole genome shotgun sequence.
ACCESSION   DBMDXG010000271 DBMDXG010000000
VERSION     DBMDXG010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7097_length_3078_cov_1.810212"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /gene="rlmD"
                     /locus_tag="ACZPD3_07710"
     CDS             complement(<1..1411)
                     /gene="rlmD"
                     /locus_tag="ACZPD3_07710"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1415911.1"
                     /translation="MKKGEIYEGVIEKVDFPNKGIVITDGQKVTVKNGMPGQKVRFMI
                     NKKRSGRAEGRLLEVLEKSPLETREPVCSIFPECGGCMYQTMSYENQLKMKECQVREL
                     LDGALNGTDYQWEGIHGSPIEFRYRNKMEFSFGDAYKDGPLTLGLHKKGSTYDILTAD
                     DCQLVHEDMTKILTCVHEYFLKRNVSYYKKMQHTGYLRHLLLRRGVTTGEILVHVITT
                     SQEEHDLESLKEELLALPLEGKIVGIMHIINDSLSDVVQSDETRILYGQDFFYETLLG
                     LRFKISTFSFFQPNSLAAEVLYSIVREYIGDTKDKVVFDLYSGTGTIAQLAASVADEV
                     IGVEIVEEAVEAAKQNAALNNLSNCKFIAGDVLKVLDDLTEKPDVIILDPPRDGIHPK
                     ALPKILSYGVDHIVYISCKATSLARDLPAFLAAGYKLEKACCVDQFCQTVHVETVVLL
                     SHKKADSYIHIDVEFGEGEG"
     gene            complement(1480..1701)
                     /locus_tag="ACZPD3_07715"
     CDS             complement(1480..1701)
                     /locus_tag="ACZPD3_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415912.1"
                     /translation="MNIKIEELLSALNRKEEEKEKNTVLWVLAIIGAVAAVAGIAFAV
                     YHFFAPDYLEDFEEDFDDDFDDYFEDEEE"
     gene            2029..2712
                     /locus_tag="ACZPD3_07720"
     CDS             2029..2712
                     /locus_tag="ACZPD3_07720"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIR1415913.1"
                     /translation="MVETLFFDLDNTLLDFNRAERAAIAKTLKSFHIAPEPSVLKRYS
                     ELNLAQWKLLEQGKITRDQVKLRRFRLLFAELNVDIPAKEAAHTYETLLAQGHYFIDG
                     AEELLETLYGQYRMYLVTNGTLSVQKGRLKSSGISRYFEDIFISEELGYNKPGREYFN
                     CCFSRIPDFHKETAVIIGDSLTSDIQGGINAGIRTIWFNPSHEKTSEIIPDHEFDSLM
                     KLPELLSHI"
     gene            complement(2790..>3078)
                     /locus_tag="ACZPD3_07725"
     CDS             complement(2790..>3078)
                     /locus_tag="ACZPD3_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase PcrA"
                     /protein_id="HIR1415914.1"
                     /translation="AQEKKKKIEEMIQADRNLAKMKMAFETKTFKPREFKVTKAATLD
                     YEVGDTVRHIKFGVGIVKDIVDGGRDYEVTVDFDKVGIKKMFAGFAKLKKI"
BASE COUNT          841 a          747 c          544 g          946 t
ORIGIN      
        1 tgccctcgcc ctctccaaac tccacatcaa tgtggatata actgtcggct tttttgtggg
       61 ataaaagaac taccgtctcc acatgaacag tctgacaaaa ttgatcaacg cagcaggctt
      121 tttccagctt atatcctgcc gccagaaatg ccggaagatc tcttgccagg ctggtcgctt
      181 tgcaggaaat atatacaata tggtctaccc cataagaaag aatcttcggc aatgctttgg
      241 gatggattcc atcccttggc ggatccagaa tgatcacatc cggtttctcc gtcagatcat
      301 ccaggacttt aaggacatct cctgcaataa atttgcaatt actcaggtta ttcagtgctg
      361 cattctgctt tgcagcttct actgcctctt ccacaatttc cacacctatc acttcgtccg
      421 ctacggaggc tgcaagctgg gcgatcgttc cggttccgct gtaaaggtcg aacactactt
      481 tatccttcgt atctccgata tactcacgta cgatagaata aagcacttcc gcagccaggg
      541 aattaggctg gaaaaatgaa aatgtactga tcttaaatcg cagtcccaga agcgtttcat
      601 aaaagaaatc ctgaccgtaa aggatacgtg tctcatcact ctgcaccaca tcagaaagag
      661 aatcattgat aatatgcatg attcccacaa ttttaccctc cagcggaagt gcaagaagtt
      721 cttctttcag agattccaga tcatgttctt cctgactggt ggtaatcaca tggaccagaa
      781 tctctcctgt agtcacaccg cggcggagaa gcagatggcg cagatatcct gtatgctgca
      841 tctttttata ataggaaacg tttcgtttca ggaagtattc atgcacacaa gtcaggattt
      901 tcgtcatatc ttcatgaacc agttgacagt catctgctgt aagaatatca taggtactgc
      961 cttttttgtg cagacccagc gtcaacggac catccttata tgcatcacca aaggagaatt
     1021 ccattttatt tcgatatctg aattcaatgg ggcttccatg aattccctcc cactggtaat
     1081 cagtgccgtt caatgcacca tccaacagtt cgcgaacctg acattctttc attttgagct
     1141 ggttttcata ggacatggtc tgatacatgc atcctccaca ttccggaaaa atgctacaga
     1201 ccggttctct tgtttcaagc ggagacttct ccaacacttc cagaagtctt ccctctgcac
     1261 gtccggaacg cttcttattg atcataaaac ggactttctg tccaggcatt ccgttcttta
     1321 cagtaacttt ttgtccgtct gttatcacaa ttcccttatt aggaaaatct actttctcaa
     1381 tcacgccttc ataaatttcg ccttttttca taaataacct ccataaaaat agccccggta
     1441 taagccggag ctattctttc attttcatat tacaggtaat tattcttctt catcttcgaa
     1501 ataatcatcg aagtcatcgt caaaatcctc ttcaaaatct tccagataat ccggagcaaa
     1561 gaaatgatat acagcaaatg cgatacctgc tactgctgca actgcgccaa tgattgccag
     1621 tacccagagg actgtatttt tttctttctc ttcttctttt ctgtttaatg ctgataataa
     1681 ttcttcaatt ttgatattca taatcgcacc atcctctcaa taattcatca gctgacactt
     1741 aacacttctg ccgtctgctg tttaatgaaa ttattataac actttttctg taattttacc
     1801 ataagatatc tgcatttatt ctatactttt gttaatttct ttattataat gatatcaggg
     1861 ggttactgtt tacacaccac tatcccgcga tgtgttttgg catgaatatg ccaaaatccc
     1921 gagacataca aaccaaaatt ccattgccgc aggcaatctg gaatgaattt tgcccctgac
     1981 ttaaaaatat gccgacagca acgatctgat aagagaggat aaatacttat ggtagaaaca
     2041 cttttttttg atcttgataa tacattactt gattttaacc gggcagaaag agcagctatc
     2101 gcaaaaactc tgaaaagttt tcacattgct cccgagcctt cagttctgaa gcgttacagt
     2161 gagctcaacc tcgcccagtg gaagcttctt gaacagggca agatcaccag agaccaggta
     2221 aagcttcgga gattccggct tctttttgca gagctgaatg tggatatacc tgcaaaagaa
     2281 gctgcacata cctatgaaac tcttctggcc cagggacact atttcataga tggtgcagaa
     2341 gaattgctcg aaactcttta cggtcaatac cggatgtatc tggttacaaa cggtacctta
     2401 agtgtccaga agggacggct taaaagttcc ggaatttccc gttactttga ggatattttt
     2461 atctccgaag aactgggata caataaaccg ggcagggaat actttaactg ctgcttctcc
     2521 agaatccctg atttccataa ggaaacagca gtaatcatcg gggacagcct gacctctgat
     2581 atccagggcg gcatcaatgc cggaatccgc acgatctggt ttaatcccag ccatgagaag
     2641 acttcagaaa tcatccctga tcatgaattt gacagtctta tgaaacttcc tgaactcctg
     2701 tctcatatat aaagcggata aaatcgttcg ccttactgct tattgctttt cgcagcctcc
     2761 gcaggtgact taagtcacct gccccattac tatatttttt tcagctttgc aaatcctgcg
     2821 aacattttct ttattcccac tttgtcaaaa tcaacagtga cttcataatc tcttccgccg
     2881 tctacaatat ctttgacaat tcctacacca aatttaatgt gtcggacagt gtctcccact
     2941 tcgtaatcca gtgtagcagc cttcgtcact ttaaattctc tgggcttgaa tgttttcgtc
     3001 tcaaaagcca ttttcatttt tgcaaggttt ctgtctgcct gaatcatctc ttcaatcttt
     3061 ttcttctttt cctgagct
//
LOCUS       DBMDXG010000272         3058 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7161_length_3058_cov_3.049374, whole genome shotgun sequence.
ACCESSION   DBMDXG010000272 DBMDXG010000000
VERSION     DBMDXG010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7161_length_3058_cov_3.049374"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(91..504)
                     /gene="tnpA"
                     /locus_tag="ACZPD3_07730"
     CDS             complement(91..504)
                     /gene="tnpA"
                     /locus_tag="ACZPD3_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpA"
                     /protein_id="HIR1415915.1"
                     /translation="MRSKRIPVEEQYRLIMECRQSGLTDHQWCVEHDIKPGTFYNWVK
                     RLRQKGCVDLPASTGRTYRAPESQEVVRVEFHDTDPIPYEQPLNVIPAATERNNFSVA
                     ESMKLSVGSFHLTIPNGTDPQLLAQTLRIVKELEC"
     gene            complement(647..2290)
                     /gene="tnpC"
                     /locus_tag="ACZPD3_07735"
     CDS             complement(647..2290)
                     /gene="tnpC"
                     /locus_tag="ACZPD3_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR1415916.1"
                     /translation="MTIKYTKEQLNKFDKDLLIELFLGMQGQMEELSRQTQALNDRMQ
                     LMMEQMVLFQKNRFGRSSEKMADSEQIRFMEVDGTIVFFNEAEAVCDLDAPEPDDLEL
                     KAPKKKKQPGKKAADIAGLTVKRIDHYLKEEELTAEFGENGWKQLPDAISRCYQFIPA
                     SVVIEEHHIGVYSSKLDEHMIKAPHPRNLLHGSLVSPSLAAAVINGKYVNAVPLYRLE
                     KEFERYGLAITRQNMANWMIRLGEEYLGTMYDYLHKLLYDYHVIQADETPVLVNKDGR
                     PAGSQSYMWVYRSGFMYRDRQIILYEYQKTRNASHPREFLRDYTGICVTDGYQVYHTL
                     EKERENLKIAGCWVHCRRRFNDALEVIPKAHRKESILHLIMKQIQAIYREEGKLSDFS
                     TEDRLMQRQLVVKPLVDAFFAYLKQNEPKIPKNGKIREAFTYALNQESYLKVFLEDGD
                     VPIDNNASERAIRGFCIGKKNWEMIDTVNGANSSAIIYSIAETAKANNLKPFDYFEYL
                     LTEIPKHVDDKNIDFLAELLPWSDMLPENIRKPQKASGK"
     gene            complement(2570..2938)
                     /gene="tnpB"
                     /locus_tag="ACZPD3_07740"
     CDS             complement(2570..2938)
                     /gene="tnpB"
                     /locus_tag="ACZPD3_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017528.6"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HIR1415917.1"
                     /translation="MLNDADASGFKKVYLAMGFTDLRRGIDGLATIVRFQFKLDPYDK
                     NTLFLFCGRRCDRIKALLWEGDGFLLMYKRLDNGAFRWPRSADAAMAITDDQYRMLMQ
                     GLEIVARHPISELKETPRIM"
BASE COUNT          754 a          748 c          619 g          937 t
ORIGIN      
        1 cctctacaat agcacacagc ccgtcaatgg atttccgcat atccgttctg cctgttacga
       61 tatagatttc atcggcggct gtgatatccc ctaacattcc agctccttca caattcgaag
      121 cgtttgagcc agaagctgag gatctgttcc atttggtatt gttagatgaa agctgcctac
      181 agacagcttc attgactctg ctacagaaaa gttattcctt tccgtagctg ccggaattac
      241 atttaatggc tgttcataag gaatcgggtc agtatcatga aactccactc tgacaacttc
      301 ctggctttct ggtgcgcgat aggtgcgtcc agttgacgcc ggcaaatcca cacaaccttt
      361 ctgacgcagt ctttttaccc agttgtaaaa agttcctggc ttgatatcat gttccacaca
      421 ccattgatga tctgtcaatc cactttgacg gcattccatg atgagacggt attgttcttc
      481 gacaggtatt cttttgcttc tcatgagcac acctccataa cttgaataga gtaaaatgct
      541 cgcatctatc atttctagca agaaaagatg aagtaaaatg cttgcattct cgtaaacggt
      601 agaatacgag taccttgaca aatagcagac cgcttttgcg gtctgtttat tttccgctgg
      661 ctttttgcgg ctttctaatg ttttcgggca acatatctga ccatggcaga agttctgcaa
      721 gaaaatctat gtttttgtca tctacatgtt tgggaatctc cgtaagcaga tattcgaaat
      781 aatcgaaagg tttcaggtta ttcgcctttg cagtttctgc gatactgtag atgatcgctg
      841 aactattggc tccattgacc gtatcgatca tctcccagtt tttcttccct atacagaatc
      901 cacggatcgc acgttcgctg gcattgttgt ctattggaac atccccatct tccagaaaaa
      961 ctttcaggta gctttcctgg ttcagtgcat atgtaaatgc ttcccgtatt ttaccgttct
     1021 ttggaatctt aggttcattc tgtttcaggt acgcaaagaa agcatccacc agtggtttta
     1081 ccacaagctg acgctgcatg agcctgtctt ccgtagaaaa atcagacagt tttccctctt
     1141 cccggtaaat ggcctgtatc tgtttcatga tcagatgcag gattgattcc tttcggtgtg
     1201 cttttggtat cacttccagt gcatcgttaa acctgcgcct gcagtgaacc cagcagcctg
     1261 caatcttcag attttcacgc tccttttcca atgtatgata tacctggtac ccatccgtaa
     1321 cacagatccc ggtgtaatcc ctcaggaatt cccggggatg ggatgcatta cgcgttttct
     1381 ggtattcata aaggatgatc tgacgatccc ggtacataaa gccggaccgg tacacccaca
     1441 tgtaactctg gctgccagca ggacgcccgt ccttatttac aagtacagga gtctcatctg
     1501 cctgtatcac atgataatca tacagaagct tatgcagata atcatacatg gttcccagat
     1561 attcttcccc aagacggatc atccagttcg ccatgttctg tctggtaatg gcaagcccgt
     1621 acctttcgaa ttctttttca agacggtaga gaggtaccgc atttacatac ttaccattta
     1681 tgactgccgc agcaagggac ggggatacaa ggcttccatg aagcagattt ctgggatggg
     1741 gtgcctttat catgtgttca tccagtttac tggaataaac gccgatatga tgttcctcta
     1801 tcacgacact ggccggaatg aactgataac atcgggaaat ggcatccgga agctgtttcc
     1861 atccgttttc accaaactca gccgtcagtt cttcctcttt cagatagtga tcaatacgtt
     1921 ttactgtcag ccctgctata tcagccgctt ttttccctgg ctgttttttc tttttgggtg
     1981 ctttcaattc caggtcatca ggttccggtg catccaggtc gcagactgcc tctgcttcat
     2041 taaagaatac gatagtcccg tctacttcca tgaaacgtat ctgttcagaa tccgccattt
     2101 tttcgctgga tctgccaaaa cggttcttct ggaaaaggac catctgttcc atcatcagct
     2161 gcatcctgtc atttaaggcc tgcgtttgcc tggacaattc ttccatctgt ccctgcatcc
     2221 caagaaaaag ctctatcagc agatccttat caaacttatt cagttgttct tttgtgtact
     2281 tgatcgtcat aaccggaact cctgccttta tactgattcc gattataaca gaatttttgt
     2341 atttccgcca atccttcaga tatgggcttt cgtcataatg acggtggata atacatctgg
     2401 aaagccgttg ttttacagct tttcagatgt attatccaca acagtttttc tattcacacg
     2461 ataacaaatg gaatgagaaa agactgtttg tggataacct ttttcatccg gttgatacac
     2521 atcattttta cagttttggt acttttttat tttcggcatt ttgcacaggc tacataatgc
     2581 ggggtgtctc tttcagctca gagatcggat ggcgtgcaac gatctccagt ccctgcatca
     2641 gcatacggta ctggtcgtct gtgatcgcca ttgccgcatc tgccgaacgg ggccaccgga
     2701 atgcaccatt atcgagtctc ttgtacataa ggagaaaacc atcgccttcc cagagcagtg
     2761 ctttgatccg gtcacatctt cttccacaga aaagaaacag tgtatttttg tcgtaaggat
     2821 caagcttaaa ctgaaagcgt acgatcgttg ccagcccatc gatcccacga cggagatccg
     2881 taaatcccat cgccagatac acctttttaa atccggaagc atctgcatca ttcagcatgg
     2941 gtaaaagcct ttatgagatg gcagagcatt tccggtgaag taccggaaaa tatctcagct
     3001 gacagtcccc tggtattttt aatacggatc attggcagag ttcctgccga aatgcatg
//
LOCUS       DBMDXG010000273         3055 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7173_length_3055_cov_1.731889, whole genome shotgun sequence.
ACCESSION   DBMDXG010000273 DBMDXG010000000
VERSION     DBMDXG010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7173_length_3055_cov_1.731889"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            52..228
                     /gene="rpsU"
                     /locus_tag="ACZPD3_07745"
     CDS             52..228
                     /gene="rpsU"
                     /locus_tag="ACZPD3_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR1415918.1"
                     /translation="MSNVIVKENETLDSALRRFKRSCAKAGIQQEIRKREHYEKPSVR
                     RKKKSEAARKRKYN"
     gene            418..1905
                     /gene="cls"
                     /locus_tag="ACZPD3_07750"
     CDS             418..1905
                     /gene="cls"
                     /locus_tag="ACZPD3_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR1415919.1"
                     /translation="MLVQLGWILMMVLRLAAYSRYIDIGLRLIGIVLVLWILNKEINP
                     SYKLAWTMLILILPILGVVLYFVFGRSRIAAIMQQHFEQRIEESREYLRDRPQTRQKL
                     EALDPSASNQSRYISDVSRFPVHENTTAEYFQVGDDMFPVLVRELKQAKKYIFIEYFI
                     INDGVMWQTILNILEKKAAEGVDVRLIYDGFGCLTTLPHKYYEELQKKGIKCQVFNPF
                     RPILNIIQNNRDHRKLCIIDGWVGFTGGINLADEYINQKARFGHWKDTAVMLKGEAVW
                     NMTVMFLHMWAVIGRSEESVDYEAYFPHRYHEEEFESDGFVQPFCDTPLDEEVVGEDV
                     YLNIINKAKKYVYICTPYLIIDNEMMTALCLAAKSGVDVRIMTPGIPDKKLVFILTQS
                     YYRQLLEAGVKIYEYQPGFLHAKSFVSDDEIGVVGTINLDYRSLYLHFEDGVWIYRNR
                     VIQDIKDDFIQTMEYCRQIEPEFCLNRNIGLRIMQNIFRAFAPLM"
     gene            complement(1994..2125)
                     /locus_tag="ACZPD3_07755"
                     /pseudo
     CDS             complement(1994..2125)
                     /locus_tag="ACZPD3_07755"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            2063..2227
                     /locus_tag="ACZPD3_07760"
                     /pseudo
     CDS             2063..2227
                     /locus_tag="ACZPD3_07760"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            2241..>3055
                     /locus_tag="ACZPD3_07765"
     CDS             2241..>3055
                     /locus_tag="ACZPD3_07765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR1415920.1"
                     /translation="MRKKWISIICAVIIMVCTIITPAYAAGIFLPEESNTKQAESTDL
                     IEAPSGILMEAQTGTVVYQKDADTRRSPASITKIMTLILIFDALDKGSLKMDDTVTTS
                     AHAKSMGGSQVFLEEGEIQTVETLIKCIVIASGNDASVAMAEHICGSEQEFVRHMNER
                     AEGLGMKNTHFEDCCGLTESPDHYTTARDIAIMSRELITKYPKILEYSSIWMENITHV
                     TRQGTKEFGLTNTNKLIRSYEGCVGLKTGSTSIAKYCLSAVAKRNDITLIAVVL"
BASE COUNT          926 a          577 c          757 g          795 t
ORIGIN      
        1 cagttgtatt ttagcacaat ctacaactgg agggaggtta ggtgtgagtc tatgtcaaac
       61 gtaatcgtaa aagagaacga gactttagat agcgctcttc gcagattcaa aagaagctgt
      121 gcaaaagcag gaatccagca ggaaatccgc aaaagagagc attacgagaa accaagcgtt
      181 cgtcgtaaaa agaaatctga agccgcaaga aaacgtaaat ataattaatt gtgcgcaaaa
      241 atccctggca gttctgtcag ggatttttat cataatttgg acaataatca ggggaaggaa
      301 gtaatcttga aggcagaaaa taacaaaata cttaaagaaa agaaaaactg gatcctttta
      361 ctgtttaaaa aactggcaaa gctgattttc aacagaatct tttatgttgc ggttgcaatg
      421 cttgtccagc tcggatggat cttaatgatg gtactgcgtc tggctgcgta ttccagatac
      481 attgatatcg gattaaggct gataggtatt gtgctggtat tgtggatttt gaataaggaa
      541 ataaatcctt cctataaact ggcctggacc atgctgatcc tgatcctgcc aatccttgga
      601 gtggtattat attttgtgtt tggacgttcc aggatcgctg caatcatgca gcagcatttt
      661 gaacagagga ttgaggagag cagggagtat ctgcgggatc ggccacagac gaggcagaag
      721 ctggaggctc tggatccctc ggcttcaaat cagtccagat atatttcgga tgtttccaga
      781 tttccggtcc atgagaacac aactgcagag tattttcagg taggagatga tatgtttccg
      841 gtgcttgtga gggaactgaa acaggcgaag aaatacattt ttattgaata tttcattatc
      901 aatgatgggg tgatgtggca gacaatcctg aatattctgg aaaagaaagc tgcggagggc
      961 gttgatgtgc gcctgatcta tgacggattt ggctgtctta caacacttcc ccataaatat
     1021 tatgaagagc ttcagaaaaa aggaatcaaa tgtcaggtat ttaatccttt ccgtcccatt
     1081 ttgaatatta tccagaataa ccgggatcac aggaagctct gcattattga cggatgggtt
     1141 ggatttacgg gaggcatcaa tcttgcagat gaatatatca accagaaagc gcgtttcgga
     1201 cactggaagg ataccgctgt tatgctcaaa ggggaagctg tctggaatat gacggtaatg
     1261 tttctccata tgtgggcagt gatcggcaga tctgaagaga gtgtcgatta tgaagcctat
     1321 tttccccaca gatatcatga ggaagaattt gagagtgacg gatttgtcca gccgttctgt
     1381 gatacacctc tggatgaaga ggttgtcggt gaagatgtat atctgaacat catcaacaaa
     1441 gcaaaaaaat atgtatatat ctgtactcct tatctgatca tagacaatga gatgatgact
     1501 gccctctgtc tggcagccaa aagcggggtg gatgtgcgta ttatgacgcc gggaatccct
     1561 gacaagaagc tggtatttat tcttacccag tcctattaca ggcagcttct ggaagccggt
     1621 gtaaagattt atgaatatca gcccggtttc cttcacgcaa aatcctttgt gagtgatgat
     1681 gagattggag ttgtgggaac catcaacctg gattatcgaa gtctttatct tcattttgag
     1741 gatggtgtat ggatctacag aaacagagta atacaggata taaaagatga ttttatccag
     1801 actatggaat attgcaggca gatagaaccg gaattctgcc tgaacagaaa tatagggctg
     1861 cgtatcatgc agaatatttt ccgcgcattt gcgccgctga tgtaaaatga gcactgaaaa
     1921 tcgtattttt atggttttca gtgcttttta tctgattatg atcaggtatc ggatcggatt
     1981 aagatgcatt attttattgg attgctatat aatgaataaa gaaagtagtc ttagagcgtg
     2041 tctgaaaaag gctttctgca agatgtgcgt cacaatttgt gggatatttt gcccggatga
     2101 gggcgccgta gcgggctacg gcaactgaat tcgggtgaaa tatcccgcaa agtggggcgt
     2161 gcagattgcg ggaatgattt ttcagacacg ctccagagcg tgcgccggca gcctgccggt
     2221 acattgatcg gagagagaat atgcgaaaaa aatggatctc cataatatgc gctgtgatca
     2281 tcatggtctg cactatcatt acccctgctt atgccgccgg aatctttttg cccgaggaaa
     2341 gcaataccaa acaggcagaa tccacagatc ttattgaagc accatcagga atcttaatgg
     2401 aagcgcagac aggaacagtc gtttatcaga aggatgcgga taccagacga agccctgcca
     2461 gtatcacgaa gatcatgact ctgatcctga tctttgatgc tctggataaa ggcagcctga
     2521 aaatggacga cacagtcaca accagtgccc atgcgaagtc aatgggcggt tctcaggtgt
     2581 ttctggagga gggggaaatc cagacagtgg aaaccctgat caaatgcatt gtgatcgcgt
     2641 caggaaacga tgccagcgta gcaatggcag agcatatctg cggcagtgaa caggaatttg
     2701 tccgccacat gaatgaacgg gcagaaggac ttgggatgaa aaatactcat tttgaagact
     2761 gctgtggtct gacagaatct cccgatcatt acacaacagc cagggacatt gccatcatga
     2821 gcagggaact gatcacaaaa tatccgaaaa ttctggaata ttcttccata tggatggaaa
     2881 atatcaccca tgtaaccagg caggggacaa aagaattcgg acttaccaat accaacaaac
     2941 tgatccgaag ctacgaggga tgtgtcggcc tgaaaacagg aagcaccagc attgcaaaat
     3001 actgcctcag tgcagtcgcc aaaagaaatg atattactct gatcgcagta gtgct
//
LOCUS       DBMDXG010000274         3054 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7176_length_3054_cov_1.655266, whole genome shotgun sequence.
ACCESSION   DBMDXG010000274 DBMDXG010000000
VERSION     DBMDXG010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7176_length_3054_cov_1.655266"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="ACZPD3_07770"
     CDS             complement(<1..399)
                     /locus_tag="ACZPD3_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1415921.1"
                     /translation="MAGMTTRELLEKLYNQDYEKLKGKKLRMTRVRVPGKEVCLAHVI
                     NPSQSCIYQNLGLHIGVHEGEDHTGEAIGMIRFTPWESVVVAADIAVKSANVEIGFMD
                     RFSGSLILTGGLTEVQTAVEEVVKFFREVLG"
     gene            complement(411..776)
                     /locus_tag="ACZPD3_07775"
     CDS             complement(411..776)
                     /locus_tag="ACZPD3_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1415922.1"
                     /translation="MANDHVEHIRIVQETVAGKEITFAHVMGGPAPVIYQKLGLNPQV
                     DYRSSAIGIMNMTPPESAVIASDIAVKSGNVYLGFADRFTGTLIITGEISDVMSAMTE
                     VVDYFRDTLGYVVCKITKR"
     gene            complement(1179..2615)
                     /locus_tag="ACZPD3_07780"
     CDS             complement(1179..2615)
                     /locus_tag="ACZPD3_07780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1415923.1"
                     /translation="MKKRIVCLTLALLMSATQVVSVSASREDELREEQAITSQQLDAT
                     YSRMDELAYAKSQLENEISELDSNLVSVMVSIDTLKGDIDNKEVDIIKTKQDLAKAQK
                     ARDKQYESMKLRIQALYEQGGDAAWFQMMLNSEDLSELLTRAENTQQMYEQDRKNLDK
                     YVNTINEVNNLKTQYESDKAELEEMKASYEQQSYDLQCQIDQKKSESADYENEIAYAQ
                     QQATEYANLLAEQTAEIQRLEAERIAAEEEARRQAEEEAARQAAEEEAAAKAAEQEQD
                     SESEEEENVEYDEDGNEIENTDDADDESESDDGVEYDENGDSVDNAGASDDVEYDEYG
                     NVIDSDNTVSPDDYESEQSSDSDSSSSSGSGSGSSVVDFATQFVGNPYVWGGTSLTGG
                     ADCSGFTQSVYANFGVSLPRTSYEQQNAGTEVSYADAQPGDLICYGGHVAIYMGNGRI
                     VHASNSVDGIKISDNAAYRTIVSVRRLV"
BASE COUNT          826 a          781 c          457 g          990 t
ORIGIN      
        1 gccaagcacc tctctaaaaa atttcaccac ttcttccact gcagtctgaa cttccgtcag
       61 acctcccgta aggatcaatg atccgctgaa gcgatccatg aaaccaatct ccacatttgc
      121 acttttcact gcaatatcag cagctacaac cactgactcc catggggtaa aacgaatcat
      181 tccgattgct tctcctgtgt ggtcttctcc ctcatggact ccaatatgca gaccaagatt
      241 ctgataaata cagctctgtg aaggattgat cacatgcgcc aggcacactt cctttcccgg
      301 aacccttacc ctggtcatac gcagcttttt tccctttagt ttctcataat cctggttata
      361 aagcttttcc agtaattctc tggttgtcat ccctgccatc ccaacacttc ctatcttttg
      421 gtaatcttgc agactacata acccagagtg tctctgaaat aatccacaac ctcagtcatc
      481 gctgacatta catcggaaat ctctcccgta atgatcagtg tgcctgtaaa tcgatctgca
      541 aatcccagat aaacatttcc gctttttacc gcaatatcag atgcgatcac tgcagattca
      601 ggcggtgtca tattcataat tccgattgca gatgaacggt aatccacctg cggattcagt
      661 ccaagcttct ggtatatgac cggagccggc cctcccatta catgtgcaaa agtaatctcc
      721 ttgccagcaa ctgtttcctg tacaattctt atgtgttcta catggtcatt tgccatttat
      781 cattcctcgc tttcactatt caactataat ccgaataatt catgagaata ttctttccca
      841 ctgatgtctc caaaaacaga tttaaaattt tatatgtatc tcttcagtta cataaactgt
      901 actatccatc caattttatg tcaaggtaaa atccacctga aaatcggtaa aatattatac
      961 atggttacgg ttcactccgt tcacagcaac acgcttcgcg atgcacagaa tttatgctga
     1021 tcgcataaat tcgcacggta tgtctcggaa ttttggcata ttcatgccaa aacacctcgc
     1081 ggaataatgg taacttatac ataaagaaaa ggagcatcag cccccttagc gatactcctt
     1141 ttcaacccct ataaatttaa ttatttatac ttatttaatt ataccagtct tcttacagaa
     1201 acaatcgttc tgtacgctgc gttatcactg atcttaattc catctacaga gttcgatgca
     1261 tgaacgatac gtccattccc catatagatt gcaacgtgtc caccatagca gattaaatca
     1321 cccggctgtg catcagcata agatacttct gtaccggcat tctgctgttc ataagaagtt
     1381 ctcggaagac ttactccgaa gtttgcatat acactctgtg taaatcctga acagtcagct
     1441 ccacctgtaa ggcttgtacc gccccataca tatggattgc caacaaactg tgttgcaaag
     1501 tctactacag aagatcctga tcctgaacct gaagatgaag aactatctga atcagatgac
     1561 tgttcactct cataatcatc tggtgaaact gtattatcag aatcaatcac attgccatac
     1621 tcatcgtact ctacatcatc agaagctcct gcattgtcaa cagaatcacc attttcatca
     1681 tattctacac catcatctga ttcagactca tcatccgcat catcagtatt ctcaatctca
     1741 tttccgtctt catcatactc tacattttct tcctcttctg attcggagtc ctgttcctgc
     1801 tctgcagctt ttgctgctgc ctcttcttct gccgcctgtc ttgcagcctc ttcctcagcc
     1861 tgtcgtcttg cctcttcttc tgctgcgatt ctttcagctt ccaatctctg aatttcagct
     1921 gtctgttccg ctaacagatt tgcatattct gtcgcctgct gctgtgcata tgcaatttca
     1981 ttctcataat cggctgattc agatttcttc tgatcgatct ggcactgaag atcataagac
     2041 tgctgttcat aagaagcctt catctcttcc agttctgctt tgtcagattc atactgagtc
     2101 tttaaattat tcacctcatt aatagtattc acatatttat caagattctt tctgtcctgc
     2161 tcatacatct gctgtgtatt ctcagcacgt gtcagaagtt cacttaaatc ttctgaatta
     2221 agcatcatct gaaaccacgc tgcatcccca ccctgttcat acagtgcctg aatacgaagc
     2281 ttcatgctct catactgctt atctctcgcc ttctgagcct tagccagatc ctgttttgtc
     2341 ttaataatat ctacttcttt attatcgata tctcctttaa gagtatcaat agaaaccata
     2401 acacttacaa gattggaatc cagttcggaa atctcattct caagctgtga tttcgcatat
     2461 gcaagctcat ccattctgga atatgtcgca tccagctgct gacttgtgat cgcctgctct
     2521 tctctcaatt catcctctct tgaagcactt acactaacaa cctgtgttgc actcatcagg
     2581 agtgctaatg taagacatac aattcttttc ttcatgaatt ctcaccctcg taacattttc
     2641 tttctatatc ttaatgtttt tgcaatatct aagttaattc ttatacatat taacacaccg
     2701 cactcaaaaa cgcaaggatt aattaccata aattttatat ttctgtaaca tttattaatc
     2761 tttttgttat tcttcgcatt tctttctaca attcaaacat attccatttc atgtttgtaa
     2821 taatttgaca caggacaaag atatctgatg cagggttact attcacacac cactatcccg
     2881 agacatacaa gccaaaattc cattgccgca ggcaatctgg aatgaatttt ggctacgtta
     2941 ctgtgaacgg agtggacagt aacgtagcag gacttcacgc tatagtggac tggattgttt
     3001 gcattattta tggaattact acagaacgca aaaaaagaag cagcctcctc attc
//
LOCUS       DBMDXG010000275         3021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7277_length_3021_cov_2.069863, whole genome shotgun sequence.
ACCESSION   DBMDXG010000275 DBMDXG010000000
VERSION     DBMDXG010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7277_length_3021_cov_2.069863"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1554)
                     /locus_tag="ACZPD3_07785"
     CDS             complement(<1..1554)
                     /locus_tag="ACZPD3_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR1415924.1"
                     /translation="MTEKQKYLLKLFREVDEICREHNLRYVLAGGSLIGALRHEGFVP
                     WDDDVDLYMPRSDWEKFIEICKTELPPDREIQCSEVDRNYTNSFPRYASTNTCAIHKS
                     QIIGKDCGGEIIDILTLDPVPADDKEYEKYRTHMMIYSDLINISVGYSDRWEIPASMY
                     LKYLLSYIFLGKKRTLAKLEKIMFSYKEEECDRYAMRWGGCPFLFDKDMMFPVKEGIF
                     EGKKAMIPNKCSDYLIWHYGDEWSYMPPHDKREGHVAVCVDDLPYQELREEYMPKINK
                     ERLRWDSVFRKFYNMRIAKKSHKVRQDGLAMKARAVALDLQRAIDESGLKISELVESR
                     SFRKLSALFGSYYKNQLSADFIGREDYTNIYAFYHPTLVEIPDDVFYAAMLTLFYTER
                     VSKAYRMMQVRQQLDHLSPEMEGLKEDIEFFRKAADHYEFHRIKEAEQIVNELLKKYP
                     GHPGFMKFKCRFLMEDAGENRIEAERFLDKALKMFPEDGYFLKYKADILWMDGEMQKA
                     AELYLQVKNK"
     gene            complement(1551..2858)
                     /locus_tag="ACZPD3_07790"
     CDS             complement(1551..2858)
                     /locus_tag="ACZPD3_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR1415925.1"
                     /translation="MKRVITYGTFDLFHKGHYNIIKRAKALGDYLIVGVTSESFDIER
                     GKLNVRDSLIKRIENVRRTGLADEIIIEEYQGQKVNDIIKYDIDVLVVGSDWRGKFDY
                     LKNYCDVVYLERTKNISSTKLRSEGVIFNMGIVTDDIRDNDFVEESKYVSGVHVERVF
                     SENHETAQRFCDKYELGSCWSSYDEFLADVDIVYIKTSLNRRAEYIERALKKGKYVIS
                     DSPMTLSSEKLRYLFQVARENNVVLIERTTLVYLRAFNQLVWLVHSNLVGDLVSVKCA
                     ISQDDFEGGRTFNETVCTAICAVLKLLGKKCQDINTNAVRNQEGRFVYDMISMKYAGA
                     LATIEIGTTVDIENELVIIGSNGRVTIPNDWWNTGYFEANISGKEFLKRYSFNFEGNG
                     FRYLLQELMIMIRDKRTECTRLFYDESVRIIEILETINRKDNS"
BASE COUNT          774 a          776 c          545 g          926 t
ORIGIN      
        1 cttattcttt acctgaagat aaagctctgc cgctttctgc atctcgccat ccatccagag
       61 gatatctgcc ttatatttca ggaaataacc atcctcagga aacattttaa gagccttatc
      121 aagaaaacgt tctgcttcga tccggttctc tcccgcatcc tccatcagga acctgcactt
      181 aaatttcata aatcccggat gtcccggata tttcttcaga agctcattga cgatctgttc
      241 tgcctccttt atccgatgga attcataatg atccgcagct ttcctgaaaa actcaatatc
      301 ttctttcagt ccctccatct caggactcag atggtcaagc tgctgacgga cctgcatcat
      361 ccgatatgcc tttgacacac gttctgtata aaacagagta agcattgccg catagaatac
      421 atcgtcaggt atctccacaa gtgttggatg ataaaaagca taaatgttgg tatagtcttc
      481 tcttccgata aagtcagcac taagctgatt cttataataa gagccaaaaa gtgcactcag
      541 cttacggaaa gatctgcttt ccaccagctc actgatctta agtcctgatt catcaatcgc
      601 tctctgcagg tccagtgcaa cagcgcgggc tttcattgca agcccatcct ggcggacctt
      661 atggcttttt ttcgcaatgc gcatattgta gaactttcgg aacacactgt cccagcgcaa
      721 tctttcctta ttgatcttcg gcatgtattc ttcacgtagc tcctgataag gaagatcatc
      781 tacacacact gctacatgac cctccctctt atcatgagga ggcatgtatg accactcgtc
      841 tccataatgc cagatcagat agtcactgca cttatttggg atcattgcct tttttccctc
      901 aaagatgccc tcctttacag ggaacatcat atccttatca aaaagaaacg gacagccgcc
      961 ccagcgcatg gcataacggt cacattcctc ttctttataa gagaacatga tcttctcaag
     1021 ctttgccagt gttctttttt taccaagaaa aatataagaa agaagatact tcagatacat
     1081 ggatgcaggt atctcccagc ggtcgctgta gcctacagaa atattgataa gatctgagta
     1141 gatcatcatg tgtgttctat atttctcgta ttctttgtcg tcagccggaa caggatccag
     1201 tgtaagaatg tctatgattt ctccgccgca gtccttcccg ataatctggc tcttatggat
     1261 ggcacaggta ttggtggatg cgtagcgcgg aaatgaattt gtatagtttc tgtccacctc
     1321 cgaacactgt atctctctgt caggcgggag ttctgttttg catatctcta tgaatttctc
     1381 ccagtccgaa cgtggcatat aaaggtctac atcatcatcc cagggaacaa atccctcatg
     1441 tctgagagct ccgatcaggg agcctcctgc cagcacgtat ctgaggttat gttctctgca
     1501 gatttcatca acctccctga aaagcttcag gaggtatttc tgtttctccg tcatgaattg
     1561 tcctttctgt tgattgtttc caaaatttct attattctta ccgactcatc atagaaaagc
     1621 cgggtacatt ctgtccgttt gtctctgatc atgatcatca gctcctgcag cagatagcgg
     1681 aaaccatttc cctcgaagtt aaagctgtac cttttcagaa attcttttcc tgaaatattg
     1741 gcttcaaagt atccggtgtt ccaccagtca ttagggatag ttaccctgcc gttgcttccg
     1801 atgatcacaa gttcattctc aatgtcaacc gttgttccga tctcaatagt cgccagtgca
     1861 ccggcgtatt tcatggaaat catatcatat acaaaacggc cttcctgatt gcggaccgca
     1921 ttggtgttga tatcctggca tttttttccc agcagtttca gtactgcgca gatagctgtg
     1981 cagactgtct cattaaaagt cctgccgccc tcgaaatcat cctgggagat tgcacatttg
     2041 acacttacca gatctcccac caggttactg tgtaccagcc ataccagctg attaaacgcc
     2101 cgcaggtaga ccagtgtggt ccgttcaatg agaaccacat tgttctccct tgccacctga
     2161 aacaggtagc gcagtttctc tgaggaaagt gtcatgggcg aatcactgat cacatatttt
     2221 ccttttttca gcgctcgctc gatatattct gctctccggt tcagcgatgt cttgatataa
     2281 acaatatcta catctgccag aaattcatca tagctgctcc agcaggaacc taactcatat
     2341 ttatcacaga atctctgtgc agtctcgtga ttctcgctga atacacgttc cacatgaact
     2401 ccgctgacat atttggattc ctccacaaaa tcattatccc ggatatcatc cgtcacgatt
     2461 cccatattaa agataacacc ttcactgcga agctttgtac tggaaatatt cttggtcctt
     2521 tccagataaa ctacatcaca gtagttctta aggtaatcga atttccctct ccagtcagat
     2581 cctaccacca gaacatcaat atcatactta ataatgtcat tgaccttctg tccctgatat
     2641 tcttcaatga tgatctcatc tgccagcccc gtccttcgca cattctcgat tctcttaatg
     2701 aggctgtccc gcacattgag tttgccccgc tcaatgtcaa agctctcact tgtgacacct
     2761 acgatcagat aatcaccaag tgcttttgct cttttgataa tattgtagtg gcctttatga
     2821 aaaagatcaa aagtgccata tgtgattact ctcttcattc tttcgtcata actcccctga
     2881 aaaaaatatt cttattttgc cgaacagttt tcctgctcca tacgttactg ttcactccgt
     2941 tcacagtaac gtagccaaaa ttcattccag attgcctgcg gcaatggaat tttggcttgt
     3001 atgtctcggg attttggcat a
//
LOCUS       DBMDXG010000276         3003 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7330_length_3003_cov_1.594762, whole genome shotgun sequence.
ACCESSION   DBMDXG010000276 DBMDXG010000000
VERSION     DBMDXG010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7330_length_3003_cov_1.594762"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="ACZPD3_07795"
     CDS             complement(<1..376)
                     /locus_tag="ACZPD3_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR1415926.1"
                     /translation="MSLARTKSKTKEPRTKITWKEIKRQKVLLFWAAIIVAYGVIFYY
                     LPLAGWAMAFQNYKPKLGILHSEFVGLEKFRTLFSDMTFIRVIRNTLAMGVINLVVTF
                     VTAIAFAILLNEIRSHIGKKVVQ"
     gene            complement(444..2114)
                     /locus_tag="ACZPD3_07800"
     CDS             complement(444..2114)
                     /locus_tag="ACZPD3_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR1415927.1"
                     /translation="MKRKKIVSMLLASAMAATLLAGCGAGGKSSSKGDSDGEIKEFTA
                     FFAVPGSEINDDNEVQQIIAEKTGAKVKETWLTGQTDVEAIGTMIAGGEYPDFIEGGN
                     GQMQLYDADALVALDDYLDDYPNIKNLFSDLEWEKLRQDDGHIYWIPQFSCIKNEEKV
                     CTHNDEAFWIQARVLKWAGYPEIKTMDDYFNLIESYNEANPTMEDGTENIPYTILCDD
                     WRYFCLENAPQFLDGYPNDGSCMVDPDTLTVLDYNTSDTAVAYFRKLNEEYQKGIVDP
                     ESFTQSYDEYISKLSTGRVLGMIDQWWDFSNANDAIKQAGLDEQGCDYIPVPVTIDGR
                     DNQWHNSGGAFNESSGLAITTSCEDVEGALQFVNDLLDQDIHNLRFWGVEGTDYEVDE
                     NGEFYRTEEERTKQSDTAYKASHTCPYSYFPQYTGTSDDGINANKPDGQASEFFDGLN
                     QDVKETFEAYGVETYVEMLGTNEAPGDWYPMWSFSNNFTTDTPGGVAWNKIADVKHEQ
                     LPQVVMAKDFDAAWDTYMDAYNACHPEDFISELQTELDTRMEQAAKFK"
     gene            complement(2257..>3003)
                     /locus_tag="ACZPD3_07805"
     CDS             complement(2257..>3003)
                     /locus_tag="ACZPD3_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1415928.1"
                     /translation="ACMLRSFQGFRARKEIYYYEDEVQVTAAGMVLCKQGLDQLLTTI
                     EQNNHMEIRKAVEQFYEEMSQMGMNAESMNLNINYLLFQLIHLASEQDNDVNQEEILR
                     LISESSFENGILRGSKAHMARFACEYGDYLSQLRKNVSRGVLGMVEKEIKENYAANLT
                     LKGLSEKYYVNSAYLGQLFRKKYGLSFKDYLNNFRMEQAAGLLIRTDKKIVQIAEEVG
                     YHDLDYFVNRFIQVKGCTPARFRKQMQQGS"
BASE COUNT          765 a          736 c          548 g          954 t
ORIGIN      
        1 tctgaaccac tttttttcct atatggcttc tgatttcatt taataaaatt gcaaacgcaa
       61 ttgccgttac gaaagttaca acaagattga taactcccat tgccagagta ttccggataa
      121 cacggataaa cgtcatatct gagaacagtg tccggaattt ttctaatcct acaaactctg
      181 aatgcaggat tcctagtttc ggtttgtagt tctgaaatgc cattgcccag ccagcaagcg
      241 gaaggtaata gaaaatcaca ccatatgcta cgataattgc tgcccagaaa agcaggactt
      301 tctgtctttt gatttctttc catgtgattt ttgttctggg ctcttttgtt tttgattttg
      361 ttctcgctaa agacatgacc tcactcctta aaaaaacggg cagatgaaat ttttcacatc
      421 tgcccgcttc aaaatttcat tttttatttg aacttcgctg cctgctccat tcttgtgtct
      481 aattcagtct gcagttcact gataaagtct tccgggtggc atgcattgta agcatccatg
      541 taggtatccc atgccgcgtc aaaatctttt gccataacaa cctgcggaag ctgctcatgt
      601 tttacatctg caattttgtt ccatgcaact ccgccaggtg tatctgttgt gaaattgtta
      661 gagaaggacc acatcggata ccagtctcca ggtgcctcat ttgttccaag catctcaaca
      721 taagtctcaa caccataagc ctcaaaggtt tcttttacat cctgatttaa tccatcaaag
      781 aactcacttg cctgtccatc cggtttattg gcgttaattc catcatcact ggttccagtg
      841 tactgtggga agtaggaata tgggcaggta tgagatgctt tgtacgcggt atcagactgt
      901 ttcgtccgct cttcctctgt tcggtagaat tctccgtttt catccacctc ataatcggta
      961 ccctctacac cccaaaatct gagattgtga atatcctgat ccagaagatc atttacaaac
     1021 tgtaatgctc cttccacatc ttcacagctt gttgtaattg caagaccgct cgactcatta
     1081 aatgccccgc ctgaattgtg ccactgattg tcacggccat caatcgtaac cggaacagga
     1141 atataatcgc agccctgctc atccagtcca gcctgtttga ttgcatcatt tgcattcgag
     1201 aaatcccacc actgatcaat cattccaagt acacgtccgg tagaaagttt ggaaatatat
     1261 tcatcgtaac tctgtgtaaa ggattccgga tctacaattc ccttctgata ctcttcattc
     1321 agttttcgga agtaggcaac cgctgtatcc gaagtgttat aatctaaaac ggtcagtgta
     1381 tccggatcta ccatacagga tccatcattt ggatatccgt cgaggaactg cggggcattc
     1441 tccagacaga aatatctcca gtcgtcacag aggatggtgt atggaatatt ttccgttccg
     1501 tcttccatag tcggattcgc ttcgttgtag gattcaatca gattaaagta atcatccatt
     1561 gttttaattt ccggataacc cgcccatttt aagactctgg cctgaatcca gaatgcttca
     1621 tcattatgtg tacatacttt ttcttcattt ttaatgcagc taaactgtgg aatccagtaa
     1681 atatggccat catcctgtct taatttttcc cactcaagat cagagaacag atttttaata
     1741 ttaggataat catccagata gtcatccagt gcaaccagtg catcggcatc atataactgc
     1801 atctgtccgt ttccgccctc aataaagtcc ggatactctc ctcctgcgat catggtgcca
     1861 atagcctcaa catccgtctg acccgtcagc caggtttctt ttacctttgc gcctgttttt
     1921 tctgcaatga tctgctgaac ctcattatca tcattgattt cggatcccgg gactgcaaaa
     1981 aatgcggtaa attcttttat ctctccgtca gaatctccct tagaggaact ctttcctcct
     2041 gctccacatc ctgcgagcag ggttgctgcc attgccgagg caagcagcat gcttacaatt
     2101 ttttttctct tcatcttgta atacctccct ttgatagtat ttattttatc agtttgcagg
     2161 tttgaaacaa tcggatgaac tatagtaaga aacagggtaa attatagatc gtagatacgc
     2221 actataattt accctgtttc ttacttcctg ttttctttaa cttccctgct gcatctgttt
     2281 tcggaatctg gccggtgtgc accctttcac ctgaataaaa cggtttacaa aataatccag
     2341 atcatgatat cctacttctt ctgcaatctg cacaattttt ttatccgtgc ggatcagaag
     2401 gcctgcggcc tgctccatcc ggaaattgtt cagatagtcc ttgaaggaaa gaccatattt
     2461 tttccgaaac agctgcccga gataggcact gttgacatag tatttttcac tgagaccctt
     2521 cagcgtgagg ttggcggcat aattctcttt gatttctttt tctaccatgc cgagaacgcc
     2581 cctggaaacg ttttttcgaa gctgagaaag ataatcgccg tattcacatg caaagcgcgc
     2641 catatgcgcc ttactgccac gcaggatacc attctcaaag ctgctttcac tgatcagccg
     2701 caggatttct tcctggttta cgtcattatc ctgctcactc gccagatgga tcagttggaa
     2761 aagcagataa ttgatattca gattcatgct ctcagcattc attcccatct gactcatttc
     2821 ttcataaaac tgttctactg ctttccggat ctccatgtgg ttattctgtt caatggtggt
     2881 aagaagctga tccagcccct gtttgcataa gaccattcca gccgctgtaa cctgcacttc
     2941 atcctcataa taatagattt cttttcgtgc ccggaacccc tgaaaagagc ggagcataca
     3001 ggc
//
LOCUS       DBMDXG010000277         2955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7451_length_2955_cov_2.017169, whole genome shotgun sequence.
ACCESSION   DBMDXG010000277 DBMDXG010000000
VERSION     DBMDXG010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7451_length_2955_cov_2.017169"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="ACZPD3_07810"
     CDS             complement(<1..869)
                     /locus_tag="ACZPD3_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415929.1"
                     /translation="MEKDNQSTQEINSQAQNPLGIAPVGGLIAKFAIPAIISMLVSAM
                     YNIVDQIFIGQGVGMLGNAATNVAFPVTTVATALALLLGIGGASNYNLEMGAGQEKKA
                     SGIAGTALSSLAISGLILAVIVLMFLKPLLTLFGATADVMPYAVDYTGITAFGLPFYI
                     LSVGGNHVVRADRSPTYSMVCIMIGAIINTILDPLFIFGFGWGIKGAAWATVIGQVAS
                     GVLVIVYFCKFRKMYLSGGMLKPKLSYLKAIISLGLASCINQIAMAIVQIVLNNILRY
                     YGANSVYGSDIPIA"
     gene            complement(970..1197)
                     /locus_tag="ACZPD3_07815"
     CDS             complement(970..1197)
                     /locus_tag="ACZPD3_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415930.1"
                     /translation="MRQTDLSIEEISLGVGYENQSYFFRQFKKRYGMILRRYRIEKGQ
                     KVCKLLKNSERVGMLFTLQGKRINVKVNDTV"
     gene            complement(1323..2657)
                     /locus_tag="ACZPD3_07820"
     CDS             complement(1323..2657)
                     /locus_tag="ACZPD3_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR1415931.1"
                     /translation="MEKEEKRADQENDTEEQSTEAKSLLKKKLQYYSSEIQRDVGNLV
                     KWLMLAVLVGGITGAASTLFSFVLKSVTNCRKENEWMFYLLPVMGLIIVYLYEKFGKD
                     DGGTNQVLSTVRSQDDVPILSAPLIFISTALTHLAGGSAGREGAAIQLGGSIANQLGR
                     WIHLDEEDRHVIVMCGMSAAFSALFGTPMAAAVFALEVVSVGVMYYTALMPCMIASLV
                     ASGFAAGMGVTPETFHVVDIPKLTIETGLKMGAIAVGCAVISIVFCMVLNGVAGVYGR
                     WFKNPYVRVVVGSCLVIGITLLLGTSDYMGAGAELIEKAVEEGQARPLDFFWKLVLTA
                     LTMRAGFRGGEIVPSFCIGATLGCVMGNWLGFSPSICAACGMTAVFCGVTNCPITSIL
                     IAFEMFGFKGVPFYLIAVSISYAASGYYGLYKDQTIVYSKYKAKYVNKPTRF"
BASE COUNT          879 a          787 c          454 g          835 t
ORIGIN      
        1 gcaatgggaa tatcacttcc atatacggaa tttgctccat aataacgcag aatattattt
       61 aatacaatct gcacgatcgc catggcaatc tgattaatgc aggatgcaag tcccagagaa
      121 ataattgctt taagatatga cagctttggt ttcagcatcc caccggacag atacattttg
      181 cggaacttac agaagtacac aattaccaga actccggaag caacctgtcc gatcactgtt
      241 gcccatgcag cacccttaat tccccatccg aatccaaaaa taaacagcgg atccagaata
      301 gtattgatga tcgcacctat cataatacag accatagaat aagtcggact tctgtctgct
      361 ctgaccacat gattcccgcc gacagacaaa atataaaatg gaagtccaaa tgcagtaata
      421 cctgtataat caacagcata aggcataaca tctgcagttg ccccgaacaa tgtcagaagt
      481 ggtttcagga acatcagtac aatcacagca agtatcagac cactgattgc cagtgacgac
      541 aacgcagtac ctgcaatccc gctggctttc ttctcctgtc ctgcccccat ttccagatta
      601 taatttgatg caccgccaat tcccaaaagc aatgccagcg cagttgcgac cgttgtaaca
      661 ggaaacgcca catttgtagc tgcatttccc agcatcccca caccctgtcc aataaaaatc
      721 tgatccacga tattgtacat cgcactgacc agcatactga tgatcgcagg aatcgcaaac
      781 ttcgcgatca atccacctac cggtgctata ccaagcggat tctgtgcctg cgaatttatc
      841 tcctgcgtac tttgattatc tttttccaag taaactccat cctctctcta catattcgat
      901 ataatttctg tcactgctat tttgtaataa aacacttttc aggataatct ctattatttc
      961 cctgccccac tataccgtat catttacttt aacatttatt ctctttccct gcaacgtaaa
     1021 aagcataccg actctttctg aatttttcaa aagtttacat actttttgtc ctttttctat
     1081 ccggtatctc cttagaatca ttccatatcg cttcttaaac tgccggaaaa aataactctg
     1141 attttcataa ccgacaccta gagaaatttc ttcaatagac aaatctgtct gcctcaactg
     1201 tcgcttttca tggctttaga ataacaaaaa taaggacagt ttttcagatt cttattaaac
     1261 tgaaaaactg tccttatttt ctatttatat ctctacaccg aacaactgct ctgcagtttc
     1321 ggttaaaacc gcgtaggttt attcacatac tttgccttat acttagaata cacaatcgtc
     1381 tggtccttat acagtccata gtatcctgaa gccgcataac tgatagaaac agcaatcaga
     1441 taaaaaggaa ctcccttaaa tccaaacatt tcaaatgcga tcagaattga tgtgatcggg
     1501 cagtttgtca ctccacagaa taccgccgtc attccacatg cagcacagat agatggcgaa
     1561 aatcccagcc agtttcccat cacacatcct aatgtagctc ctatacagaa cgaaggaaca
     1621 atttccccgc ctcggaatcc tgcacgcatt gtcaatgcag tcagtaccag tttccagaag
     1681 aagtccagag gtcttgcctg tccttcctcc actgccttct caatcagctc tgcacctgcg
     1741 cccatataat ctgaagttcc cagaagtaat gtaattccaa tcaccagaca ggatcccaca
     1801 actacacgca catatggatt cttaaaccac cttccatata ctccggcaac tccattcagc
     1861 accatacaga atacaatact aatcactgca catccaacag cgatagcacc cattttcaaa
     1921 cccgtttcaa tagtcagctt cggaatatcc actacatgaa aagtctccgg tgtaactccc
     1981 attcctgccg caaatccgga tgcaaccagg gaagcaatca tacaaggcat caaagctgta
     2041 taatacatca ctcctacact gactacctcc agtgcaaaaa cagccgcagc catcggtgtt
     2101 ccaaacaatg cagagaatgc tgcactcata ccacacataa caattacatg tcggtcttcc
     2161 tcatccagat gaatccaacg tccaagctga ttggcaatac ttccaccaag ctgtattgcc
     2221 gcaccttcac gtcctgcaga gccacctgcc agatgcgtaa gtgctgtaga aatgaaaatc
     2281 agaggtgcag ataaaatcgg cacatcgtcc tgtgatctca cagtagaaag cacctgattc
     2341 gtacctccat catccttacc aaatttctca tacaggtata caatgattaa tcccatcacc
     2401 ggaagcagat agaacatcca ttcattttct ttcctgcaat ttgtcacact cttcagcaca
     2461 aaagaaaaca gtgtactcgc cgctcctgta atacctccta caagtaccgc gagcatcagc
     2521 cattttacaa gatttcccac atcacgctga atctcactac tataatattg aagctttttt
     2581 ttcagtaagc ttttcgcttc cgtactttgt tcttccgtgt cattctcctg atctgcacgc
     2641 ttttcctctt tctccatttc cccctcttac ctttccttat tattttacat acaaatttgc
     2701 tatttctatt ttagcataca ttttccatat ctgccagaaa gataagacag agggattaag
     2761 gattattgaa ttattggaag atatttgatt cggttaaaat gcagtacata tattaacgtt
     2821 tttcccaacg tcttccttct ctgcaaaagg tccgggaatt accttggcac ctctgtgtcc
     2881 tccaaagtaa tcggtatcaa atgttatcgg tgttccatcc ggattttcga agaattcttc
     2941 cggctcaaaa gcttt
//
LOCUS       DBMDXG010000278         2954 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7459_length_2954_cov_1.669120, whole genome shotgun sequence.
ACCESSION   DBMDXG010000278 DBMDXG010000000
VERSION     DBMDXG010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7459_length_2954_cov_1.669120"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2441
                     /locus_tag="ACZPD3_07825"
     CDS             <1..2441
                     /locus_tag="ACZPD3_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR1415932.1"
                     /translation="SAQQEADRFTDRQEDSELTAGFMWQTLKRTSRELGLISTVTSFI
                     PKSDNVEIMYVTVENQTDTVQKFTAYGAIPVYGRSADNIRDHRNVTSMLHRIETTEHG
                     INVCPTMSFDERGHQPNHKIYYVNGCSGKGENPISYYPTVEDFIGEGGTYTHPRTVYE
                     GYKGVPAHSLAAGKEAMGAFCFSSFTLAPGEKTDFILLSGIADSKEEIENIFEKYNTS
                     DKVKNALEETRIYWKKQVNVDFHTQDPDFDSYMKWVSFQPFLRRLFGCSFLPHHDYGR
                     GGRGWRDLWQDCLSLLLMNPQNVGAMIEKNYGGVRIDGTNATIIGDGDGNFIADRNGI
                     ARVWMDHALWPLITTSLYINQTGDIEILKKQVPYFKDAQTMRGTEIDTLWNDAYGNKQ
                     RTEDGQVYTGSVLEHILIQQLAAFYDVGVHNIYRLRGADWNDALDMAAENGESVAFTC
                     AYAGNLHTLASILRLMESAGETSIPLSEEIEILLNDQTDMFDSVSEKKKILTEYAKSC
                     RHNLSGRKKNFSLSTLAANLIQKSNWLTDHIRSQEWIDGKDSEEGWYNSYYDNHGEAV
                     EGFHHEQVRMMLTGQVFSIMGNVATDAQIRKIVKSADHYLYRKEIGGYRLNTDFQELK
                     FDMGRMFGFAYGEKENGAVFSHMAVMYANALYQRGFAKEGWKALRSLSDTALDFDTSH
                     IYPGIPEYFRSDGRGMYHYLTGAASWYLFTMITEVFGVRGVFGNLLVHPKLLAEQFDE
                     AGTASVSVTFAGKQFHVTYRNTDRKDYGSYHIAAADCDKTAIEIVEDSHIMISREFIN
                     NLSSDLHEITVTLA"
     gene            2522..>2954
                     /locus_tag="ACZPD3_07830"
     CDS             2522..>2954
                     /locus_tag="ACZPD3_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR1415933.1"
                     /translation="MKYGYFDNENREYVITNPATPAPWVNYLGSPEYGAIISNNAGGY
                     SFAKSGANGRILRYVFNNFDQPGRYIYIRDDRSSDFWSASWQPAGKDLAQYKSECRHG
                     IGYTKISADYSDIHSEALYYVPLGKSYEVWALSVTNHSDHER"
BASE COUNT          885 a          620 c          658 g          791 t
ORIGIN      
        1 cctctgctca gcaggaagcc gaccgtttta ctgacaggca ggaagacagt gaactaaccg
       61 ccggattcat gtggcagact ttgaaaagga cttccagaga acttgggctc atctccacag
      121 ttacttcttt tatccccaaa agtgataatg tggagattat gtatgtgacg gttgaaaacc
      181 agacagatac tgtacagaaa tttactgcat atggtgcaat tcctgtttac ggaagaagtg
      241 cggataacat tcgtgatcat cgaaatgtca cttccatgct ccatcgtatc gagacaacag
      301 aacatggaat caacgtctgc cccaccatgt catttgacga gcggggacat cagccaaatc
      361 ataaaatcta ttatgtaaat ggctgttccg gaaagggaga aaatccgatt tcctattatc
      421 cgactgtaga agattttatc ggagaaggtg gtacctatac acatccacgc acagtatatg
      481 aaggatataa aggagttccc gcacacagtc tggcagcagg aaaggaagca atgggtgctt
      541 tctgtttctc atctttcaca ctggctcccg gtgaaaaaac agactttatt ttattatcag
      601 gtattgccga cagtaaagaa gaaattgaaa atatttttga aaaatacaat acttccgata
      661 aagtaaaaaa tgcactggaa gaaaccagaa tttactggaa aaaacaggta aacgttgact
      721 tccacacaca ggatcctgac tttgacagtt atatgaaatg ggtaagtttc cagccattcc
      781 tgcgccgttt atttggctgt tctttccttc ctcatcatga ttacggacgt ggtggacgcg
      841 gatggcgtga tctgtggcag gattgtcttt ctcttctgct gatgaatcct cagaatgtag
      901 gtgccatgat tgaaaagaat tatggcggcg tacgtattga tggtacaaat gctactatta
      961 tcggagacgg tgacggcaat tttatcgctg acagaaacgg aatcgcacgt gtatggatgg
     1021 atcatgcact ctggccgcta atcacgacca gtctttatat taatcagacc ggagatatag
     1081 agatccttaa gaaacaggtt ccttatttta aagatgcaca aaccatgcgc ggcacagaaa
     1141 ttgatactct ctggaatgac gcatatggaa ataaacagcg caccgaagac ggtcaggtat
     1201 ataccggaag tgttctggag cacattctga tccagcagct tgccgctttt tatgatgtag
     1261 gtgttcataa tatttaccgt ctccgcggag ctgactggaa cgatgccctg gatatggcag
     1321 cagaaaatgg tgaaagtgtt gcctttacct gtgcttatgc gggcaatctc catactcttg
     1381 cttccatatt acgccttatg gaatctgcag gcgaaaccag tatcccgctg tcagaggaaa
     1441 ttgaaatcct gctgaacgac cagacagata tgtttgacag tgtatctgaa aagaaaaaga
     1501 ttcttactga atatgccaaa agttgcaggc ataacctaag cggcagaaag aaaaacttct
     1561 ctctctccac acttgctgcc aacctgatcc agaaatccaa ctggctgaca gatcatatcc
     1621 gttcacagga atggattgat ggcaaagaca gcgaagaagg atggtataac agctattacg
     1681 ataatcatgg agaggcagtg gaaggtttcc accatgaaca ggtgcgtatg atgctcacag
     1741 gccaggtttt ctctattatg ggaaatgttg ccactgatgc gcagatcagg aaaatcgtaa
     1801 aaagcgcaga tcattatctt tacagaaaag aaattggcgg ttaccgcctg aacacagatt
     1861 ttcaggaact gaagtttgat atgggacgta tgttcggttt cgcttatggc gaaaaagaaa
     1921 acggtgctgt tttctctcat atggcagtta tgtatgcaaa tgccttatat cagagaggct
     1981 ttgcaaaaga aggctggaaa gcattgcgtt ctctgtctga tacagctctc gactttgata
     2041 ccagccatat ttacccggga attcctgaat atttccgttc agacggacgt ggcatgtatc
     2101 attatctgac aggtgctgca agctggtatc tttttaccat gatcacagag gtctttggtg
     2161 tccgtggagt tttcggaaac cttctggttc atccaaaact tctggcagaa cagttcgatg
     2221 aagcaggaac tgcttctgtc tctgttactt ttgcaggaaa acagttccat gttacctacc
     2281 gaaacacaga cagaaaagat tatggttctt atcatatcgc cgctgcggac tgcgacaaaa
     2341 cagcaataga aatcgtagaa gattcccata ttatgatctc cagagaattt attaacaacc
     2401 tctcttctga tcttcatgaa attacagtga cacttgcctg aaaacaatag ctgctcagcc
     2461 tccataaaag cagtcgacag gcgcagttat aaatattgtt ctaacagaaa ggatttacta
     2521 tatgaaatac ggatattttg acaatgaaaa ccgagaatac gtgattacta atccggcaac
     2581 acctgcacca tgggtaaact atctgggttc cccggagtac ggtgcaatca tttccaataa
     2641 tgcagggggc tacagttttg caaaatccgg tgcaaacgga cgtatcctgc gctatgtttt
     2701 taataacttt gaccagccgg gacgttacat ttacattcgc gatgacagat catcagactt
     2761 ctggtctgca tcctggcagc ctgcaggcaa agatctggct cagtataaga gcgaatgccg
     2821 tcacggaatc ggttatacaa aaatttctgc cgactacagc gatattcact cagaagccct
     2881 ttactatgtt cctcttggaa aaagttatga agtatgggca ttatctgtga ccaatcattc
     2941 tgatcacgaa agga
//
LOCUS       DBMDXG010000279         2923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7545_length_2923_cov_1.369950, whole genome shotgun sequence.
ACCESSION   DBMDXG010000279 DBMDXG010000000
VERSION     DBMDXG010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7545_length_2923_cov_1.369950"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="ACZPD3_07835"
     CDS             complement(<1..1541)
                     /locus_tag="ACZPD3_07835"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HIR1415934.1"
                     /translation="MEKRWWKESVVYQIYPRSFCDSNGDGIGDLNGITGKLDYLKELG
                     IDVIWLSPVYKSPNDDNGYDISDYQAIMDEFGTMEDFDRMLATAHEKGIKIMMDLVVN
                     HTSDEHKWFIESRKSTDNPYRDYYIWRPAKEDGSLPNNWGSCFSGPAWEYDKTTDMYF
                     LHLFSKKQPDLNWDNPAVRQDVFDMMNWWLKKGVDGFRMDVISLISKEPGLPDKETGI
                     NGYATFNVSANGPHVHEYLQEMRQKALNNADTITVGECSGVTLEEAKKYARSDEKELN
                     MVFQFEHMDVDSDEKAGKWTTRKMDLRNLKKILTRWQKGLQDIAWNSLYWENHDQPRS
                     VSRFGNDSDEYREISAKMLATCIHMMQGTPYVYQGEELGMTNCPFNTLDNFRDLESIN
                     AFHELTEQGKMTEEDMMAAIGYKGRDNARTPMQWDDSAYAGFSTTNPWIMVNPNYTKI
                     NAKDQVNREDSVFKYYQKLIKLRHESELIVYGTYDLILDDDKDIYAYIRTLGDKKLIV
                     YCNFS"
     gene            1925..2899
                     /locus_tag="ACZPD3_07840"
     CDS             1925..2899
                     /locus_tag="ACZPD3_07840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR1415935.1"
                     /translation="MRRIQIIVNNGAGTGRAHRVWNETQCLLRGYKIKYEAHMTRYEG
                     HAAKLAEQISRVKGEEPVYLLVVGGDGTINEVLNGITDFDKVRFGVIPTGSGNDFGRN
                     LKLPKTPKESLREICACIRKDQRGEALYRIDLGQVSWEGCEKPRIFGISSGLGLDALV
                     CKKALHSRLKQVLNRFHLGKLTYLALTVQSLFTMETANAKVVTEHGGYILPKMIFAAA
                     MNLPAEGGGVPMAPHASVQDGLLSLGSASGIAKWQTFFLLPFLVAAKQEHINGFTIRN
                     EKGFRLILDKPMVLHADGEYCADVTRVEFRCLEKKLWLLHEQKGVISS"
BASE COUNT          772 a          569 c          695 g          887 t
ORIGIN      
        1 tcgctgaaat tgcagtatac gatcagtttt ttatcgccaa gtgtacggat ataagcgtag
       61 atatccttgt catcatcaag gatcagatca taggtaccgt atacgatcag ttcactttca
      121 tggcggagct tgatcagttt ctgataatat ttgaatacgg aatcttcgcg gttaacctga
      181 tctttggcat tgatcttagt gtagttcggg tttaccataa tccaggggtt agttgtggag
      241 aaacctgcgt aagcactgtc gtcccactgc ataggggttc ttgcattgtc acgtccctta
      301 taaccgattg ctgccatcat gtcctcttcg gtcatcttac cctgttcggt taattcgtgg
      361 aaagcattga tactttcaag gtcgcggaag ttgtcaagag tgttaaacgg gcagtttgtc
      421 atacccagtt cctcaccctg atatacataa ggtgtaccct gcatcatatg gatacatgtt
      481 gcaagcattt ttgcagagat ttctctgtat tcgtcactgt cattgccgaa acgggaaacg
      541 cttcttggct ggtcatgatt ttcccagtag agactgttcc atgcaatatc ctgaagacct
      601 ttctgccagc gtgtaaggat ttttttcagg ttgcgcagat ccatctttct ggttgtccat
      661 ttgccggctt tttcatcact gtcaacatcc atatgctcaa actggaatac catgttcagc
      721 tctttttcat cgcttcttgc atatttcttt gcctcttcca gagtaacacc tgagcattca
      781 cctacagtga tcgtgtcagc attattcagt gctttctgac gcatttcctg aagatattca
      841 tgtacatgag ggccatttgc ggatacatta aaggttgcgt aaccgttaat tccagtttct
      901 ttgtcaggaa gccccggctc tttggagatg agactgataa cgtccatacg gaatccgtca
      961 actccctttt tcagccacca gttcatcata tcgaaaacat cctgacgtac agctggattg
     1021 tcccagttca gatcaggctg tttcttggag aacaggtgca ggaaatacat atctgttgtc
     1081 ttgtcgtatt cccatgcagg accggagaag caggaacccc agttgttcgg aaggcttccg
     1141 tcttcttttg caggacgcca gatgtagtaa tctctgtaag gattgtctgt ggatttgcgg
     1201 ctctcgatga accatttgtg ttcatcagag gtatggttta caaccaggtc catcatgatt
     1261 ttgatgcctt tttcgtgagc tgtggcaagc atacggtcga agtcttccat ggttccgaat
     1321 tcgtccatga tcgcctgata atcactgata tcatatccgt tgtcatcatt tggtgatttg
     1381 taaaccggag agagccagat tacgtcaatt ccaagctctt ttaaatagtc aagtttgccg
     1441 gtgataccgt taagatcacc gattccgtct ccgttgctgt cgcagaaact gcgaggatag
     1501 atctgatata cgacgctttc tttccaccat cttttttcca ttgtaaagac ctcctgataa
     1561 tattatattt tagtacgttt gatttgaata agaattatgt gagctgacta taacaatcct
     1621 ctcaatacgc attatttacg aggattgctg taaatatctg ttgctcctga cactcttgaa
     1681 tttgtgtata gttatattgt ataaatatat aataaacacc ttatggagaa aatgctttca
     1741 atattttgat ggatttatat attattttga ctttttgaca aaatcataga attgtaacga
     1801 aagaggattt cgcagtttcg aagtcttgta tgtgatgggt aaatattgta tagtataggt
     1861 attggcattt ataatgagat agaagaaatg gcagaaaata aatataatgt ataagtgagg
     1921 aagaatgaga aggatacaga ttattgtaaa taatggagcg ggtacagggc gggcgcacag
     1981 ggtatggaat gagacacagt gtctgcttcg gggatacaaa ataaaatatg aagcccatat
     2041 gaccagatat gaagggcatg ccgcgaaact ggctgagcag atttcccggg tgaagggtga
     2101 agagcctgtc tatctgctgg tggttggggg agacggtacg atcaatgaag tcctgaatgg
     2161 tattactgac tttgataagg tacgttttgg cgtgataccc actggttcag ggaatgattt
     2221 tggcagaaat ctgaaactgc cgaaaacacc gaaggagagc ctgagggaaa tctgtgcctg
     2281 tatcagaaag gatcagagag gtgaagcact ttacaggata gatctggggc aggtaagctg
     2341 ggaaggctgt gagaagccgc ggatttttgg aatcagttcc ggcctgggac tggatgcact
     2401 tgtgtgtaaa aaagcacttc attccagact gaaacaggtt ttaaaccgtt tccatctggg
     2461 taagcttaca tatcttgcat tgacggtgca gtctcttttt accatggaga cagccaatgc
     2521 aaaggtggtg acagaacatg gaggctatat tcttccgaag atgatctttg cggcagctat
     2581 gaatctgccg gcagaaggcg gaggggttcc tatggcacct catgcatcgg tgcaggacgg
     2641 tctgctctcg cttggcagtg catccggaat cgccaaatgg cagacttttt tcctgcttcc
     2701 gtttctggtt gctgcaaagc aggagcatat taatggtttt actatcagaa atgaaaaagg
     2761 atttcgcctg attctggata agccaatggt gctccatgca gacggagagt actgtgcaga
     2821 tgtgacgaga gtggaatttc ggtgtctgga gaagaaactg tggctgctgc atgagcagaa
     2881 aggggttata agcagttaaa tataaattat aaaattatat aag
//
LOCUS       DBMDXG010000280         2923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7546_length_2923_cov_1.335223, whole genome shotgun sequence.
ACCESSION   DBMDXG010000280 DBMDXG010000000
VERSION     DBMDXG010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7546_length_2923_cov_1.335223"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="ACZPD3_07845"
     CDS             complement(<1..1364)
                     /locus_tag="ACZPD3_07845"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR1415936.1"
                     /translation="MAVKYVFVTGGVVSGLGKGITAASLGRLLKARGYQVTMQKFDPY
                     INIDPGTMNPIQHGEVFVTDDGTETDLDLGHYERFIDESLDKNSNVTTGKVYWSVLQK
                     ERHGDFGGGTVQVIPHITNEIKSRFYRAKSPEENKIAIIEVGGTVGDIESQPFLEAIR
                     QFQLNVGHDNAILIHVTLIPYLKASEELKTKPTQASVKELQGMGIQPDVLVCRSEHEL
                     TEDIKEKIALFCNVPVSHVLQNLDVEYLYEAPLAMEREKLADVVLSSLRLENRKPDLS
                     DWEEMVESLRNPNKTVKIAIVGKYTQLHDAYLSVVEALKHGGISCRAKVELDWIDSEE
                     LTEKNLDQQLHNVDGILVPGGFGNRGTEGMILAAQYARVHKIPYLGICLGMQMAIVEF
                     ARHVLGYEDANSIELNPETKHPVIALMPDQEDIEDIGGTLRLGSYPCILAEGSRSYEL
                     FGKK"
     gene            complement(1738..1965)
                     /locus_tag="ACZPD3_07850"
     CDS             complement(1738..1965)
                     /locus_tag="ACZPD3_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1415937.1"
                     /translation="MSQSKIKLNATEEVQEFVNAASRCDFDIDVYYNRFLIDAKSILG
                     VLSLDLTRELTVDCHGESREFDRTLKKFAVA"
     gene            complement(2235..>2923)
                     /locus_tag="ACZPD3_07855"
     CDS             complement(2235..>2923)
                     /locus_tag="ACZPD3_07855"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR1415938.1"
                     /translation="KGLELGRKGTIAVNDNMQSSIPTIFAGGDIVRGGKSLAKSYPVE
                     ADLVTGVPESGLPAAKGYSEASGIPFAFAFYKNSYIGRTFIKPTQEERESSVHLKLSV
                     LESVVKDKRIVLVDDSIVRGTTIANLIHMLKEAGAKEVHVRISSPPFLHPCYFGTDVP
                     SNDQLIAASHSTEEICKMIGADSLGYMQTDYLEGMAGGLPLCKACFDGNYPMEIPDYT
                     NAEFADIVKC"
BASE COUNT          762 a          693 c          511 g          957 t
ORIGIN      
        1 tcttttttgc caaagagttc ataggatctg gaaccttctg caagaataca tggataactt
       61 ccaagtctta aggttccacc gatatcttcg atatcttcct gatccggcat taatgcaatt
      121 accggatgct ttgtctccgg attcagttca atactgtttg catcttcgta tccaagtaca
      181 tgacgggcga attctacaat tgccatctgc attcccagac agattccaag atatggaatc
      241 ttatgtactc ttgcatactg tgctgccagg atcattcctt ctgtacctct gttcccgaaa
      301 ccacccggaa ccaggatacc atctacattg tgaagctgct gatccagatt cttctctgtc
      361 agttcttcgg aatcgatcca gtcaagctct accttggctc tgcaggaaat tccgccatgt
      421 ttcagtgcct ctactacact gagatatgca tcatgaagct gtgtatattt tcctacaatt
      481 gcgatcttta ctgttttatt cggatttctc agactttcta ccatttcctc ccagtcactt
      541 aaatccggtt ttctgttttc cagacggaga cttgaaagaa ctacatctgc aagcttctct
      601 ctttccattg caagaggagc ttcatataaa tattccacat ccaggttctg aagtacatgt
      661 gatacaggta cgttgcagaa taaagcgatt ttttctttaa tgtcctctgt cagctcatgt
      721 tcacttctgc acaccagtac atccggctga attcccattc cctgaagttc ctttacgctt
      781 gcctgtgtag gttttgtttt cagttcttca gatgccttta aataaggaat taatgttacg
      841 tgaatcagga ttgcattgtc atgtcccaca ttgagctgga actgacgaat tgcttccagg
      901 aacggttggc tttcaatatc accaactgtt cctcccacct cgataatggc aatcttgttt
      961 tcctccggtg atttggcgcg gtagaaacga ctcttaatct catttgtaat atgaggaatt
     1021 acctgtacgg tacccccgcc gaaatccccg tgacgttctt tctgaagaac agaccagtaa
     1081 accttaccgg ttgtcacatt ggaattttta tcaagactct catcaatgaa tctctcataa
     1141 tgtcccagat ccaggtctgt ctctgtacca tcgtctgtta caaaaacttc tccgtgctga
     1201 atcgggttca tggttccggg gtcgatattg atataaggat caaacttctg catggttacc
     1261 tgatatcctc tcgctttcag aagacgcccc agagatgcag ctgtgattcc ctttccaaga
     1321 ccggaaacaa ctcctcctgt aacaaatacg tatttcactg ccattttgat ttcctcctcc
     1381 atcatccttt gttaataaaa aagcgtcaga tgcagctatc agagggcact tgacccatct
     1441 ggtattctgc atctgacgcc tttgtcagat catcatatct tctataataa tatgttcaat
     1501 tggctttctt taaaaatatg ctatactata aaacattttt tttcgtttgt caataatgaa
     1561 tttcccgttt ttaagcaaaa acaaatcaaa taagttcctt ctgataaact atgaagcagt
     1621 tatattttat ggaattactc tgaacacagt cataatacag aaaaaacgaa tctgtagaag
     1681 ttcatcttcc agacccgtct tctctgcgat aattattcgg ttataaaaat gtaaaattta
     1741 tgcaactgca aatttcttca atgtacgatc aaattcccgg ctctccccgt ggcagtctac
     1801 tgtcagttct ctggtcagat ccagactaag tacaccaagg attgatttgg catcaattaa
     1861 aaatctgttg taataaacat caatatcaaa atcacatctg cttgcagcat tcacaaattc
     1921 ctgcacctct tcggttgcat ttaacttgat tttactctgt gacatcgtga tcaccctttc
     1981 tgaaaacatt ctttgttttt ttctatcaga cgcccatatt atgttccaca tttcagaatt
     2041 tgtcaagttt ttaaatttat ccaattttca taaagcgcag gggttctatt gtgcattttg
     2101 ttcgtttata tatgttttca cagcattatt ttatacatta tgtatgactt catcctatat
     2161 tggttttacc attatgtgag attgattcca tatatcatcg acaggtttct gcatttttta
     2221 tgaatttttg cgacttaaca cttcacaata tctgcaaact ccgcatttgt ataatccgga
     2281 atctccatcg gataatttcc atcgaagcat gccttacata agggcaggcc gcccgccatt
     2341 ccttccagat aatctgtctg catgtagcca agagaatctg ctccgatcat tttacagatt
     2401 tcctccgtgc tgtggctggc tgcaatgagc tgatcatttg acggcacatc cgttccgaaa
     2461 taacatggat gtaaaaatgg cggtgaactg atacgtacgt gcacttcctt tgctcccgcc
     2521 tcttttaaca tgtggatcag gtttgcaatg gttgttccac gaacaataga atcatccacc
     2581 agaacaatcc tcttatcttt tacaacagat tccagcacac tcagtttcag atgaacgctg
     2641 gactcacgtt cttcctgagt cggtttgata aatgttcttc caatatagct gtttttatag
     2701 aacgcaaatg caaacgggat tcctgatgct tcggagtatc cctttgcagc agggagtccg
     2761 gactccggta cacctgttac aagatctgcc tctacaggat aagacttggc aagagattta
     2821 ccaccgcgga caatgtctcc tccggcaaag atggtcggga tggacgactg catgttatcg
     2881 ttgacggcaa tcgttccttt ccggcccaat tccagtccct tga
//
LOCUS       DBMDXG010000281         2918 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7561_length_2918_cov_1.424920, whole genome shotgun sequence.
ACCESSION   DBMDXG010000281 DBMDXG010000000
VERSION     DBMDXG010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7561_length_2918_cov_1.424920"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /gene="argS"
                     /locus_tag="ACZPD3_07860"
     CDS             complement(<1..1130)
                     /gene="argS"
                     /locus_tag="ACZPD3_07860"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR1415939.1"
                     /translation="MKKLMEQMAEELSAAFEKSGYDPSYGKVTVSNRPDLCEFQCNGA
                     MAGAKAYKKAPFMIADDVVAYLKDSQVFSMAEVVKPGFINLKVKGEFLADYLKEMAAD
                     EKLSVSAAKEPKTIIIDYGGPNVAKPLHVGHLRSAIIGESIKRIGRFVGHKVIGDVHL
                     GDWGLQMGLIITELKHRKPELVYFDDSYTGEYPEEAPFTISELEEIYPCASGKSKEDE
                     AYRNEALEATHLLQQGKPGYMALWNHIMNVSVTDLKRNYDKLNVSFDLWKKESDAQPY
                     IPGMVEEMKEKGFAYVDQGALVVDVKEENDTKEIPPCMLLKSDGASLYTTTDLATIVE
                     RMKLFNPDEILYVVDKRQELHFIQVFRCARKTGLVKDDTKLS"
     gene            complement(1155..2027)
                     /locus_tag="ACZPD3_07865"
     CDS             complement(1155..2027)
                     /locus_tag="ACZPD3_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1415940.1"
                     /translation="MGKIAIVTDSNSGITQDQARELGISVIPMPFYINEKLYLEGITL
                     SQEEFYERLKNDEAISTSQPGPADVCGLWNTLLEKYDEVVHIPMSSGLSASCDTAMGL
                     AQEYDGRVHVVDNQRISVTQRQSVLDALTLRDAGRNATQIKQVLEEQKLDSSIYITLE
                     TLKYLKKGGRITPAAAAIGTVLNLKPVLQIQGEKLDAYSKTRGKKQAKRVMLKAMQND
                     WENRFAEYVKRGEMCLQSAYTGNQEEAEEFKKEIAEVFPEIEIVQNPLSLSVACHIGH
                     GAIAVACSRKVVVE"
     gene            complement(2236..>2918)
                     /locus_tag="ACZPD3_07870"
     CDS             complement(2236..>2918)
                     /locus_tag="ACZPD3_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR1415941.1"
                     /translation="IYKAVAQNMAGKKVIIRTLDIGADKQIDYFDMAHEENPAMGYRA
                     IRICLDRPEVFKTQLRALFRASMFGNISIMYPMIISVTEVKQIKAIVAEVKKELTEQG
                     IPFKDDVEQGVMIETPAAVMISDLLAKEVDFFSIGTNDLTQYTLAIDRQNAKLDNIYD
                     SHHEAVLRMIQMVIDNAHKEGIWAGICGELGADTTLTERFIQMGIDELSVSPTFVLPV
                     RKIVRELD"
BASE COUNT          711 a          701 c          529 g          977 t
ORIGIN      
        1 aaagaaagtt ttgtatcatc ttttacaagc ccggtctttc ttgcacagcg gaatacctgg
       61 ataaagtgaa gttcctgacg tttgtctacc acatacaaga tctcatccgg gttaaataat
      121 ttcattcgct ctacgattgt tgcaaggtct gtagttgtat aaagagaagc tccgtctgat
      181 ttcagaagca tacatggagg aatttctttt gtatcgttct cttcttttac atctactaca
      241 agtgcgccct ggtctacata ggcgaatcct ttttctttca tctcctcaac cattccggga
      301 atatatggct gtgcatcgga ttcctttttc cagagatcaa aagacacatt taatttgtca
      361 tagttgcgct tcaggtctgt aacggatacg ttcataatat ggttccagag agccatgtat
      421 cccggtttgc cctgctgaag aagatgagtt gcttccagag cttcgtttct gtacgcttca
      481 tcctctttgg acttgccgct tgcacatgga tagatctctt ccagctcgct gatcgtaaat
      541 ggtgcttcct ccggatactc tcctgtgtaa gaatcatcaa aataaaccag ctccggtttt
      601 ctgtgtttca gttctgtaat gatcagtccc atctgaagac cccagtctcc cagatgaaca
      661 tcaccgatca ctttatgtcc tacaaagcga ccgattctct taatactttc tccaatgatt
      721 gcagaacgaa ggtgtcctac atgaagaggt ttcgcaacat ttggtccgcc ataatcgatg
      781 atgatcgtct tcggctcttt tgctgcagat acggaaagtt tctcgtctgc tgccatttcc
      841 tttaagtaat ctgcaaggaa ttcacctttt acttttaaat tgataaatcc cggttttact
      901 acttctgcca tggagaaaac ctggctgtct tttaagtatg ctactacatc atcagcgatc
      961 ataaacggtg ccttcttgta tgctttggct cctgccatag caccgttaca ctgaaattcg
     1021 caaagatccg gtctgttgga tacggttact tttccgtagg atgggtcgta tccggatttt
     1081 tcaaatgctg cggaaagttc ttccgccatc tgttccataa gtttcttcat tctttcctaa
     1141 ctcctcagat tgttttattc tactaccact tttcttgaac atgcaactgc aatagcacca
     1201 tgtccgatat ggcatgcaac acttaatgat aacggattct gcacaatctc aatttcgggg
     1261 aatacttcgg caatttcttt cttaaattcc tctgcttcct cctgatttcc tgtataagca
     1321 gactgcaggc acatttcgcc acgttttacg tattctgcga aacggttctc ccagtcattc
     1381 tgcatagcct tgagcatgac acgttttgcc tgcttcttgc ctcttgtttt ggaatatgca
     1441 tccagcttct caccctggat ctgaagcaca ggtttcaggt tcagaactgt tccgattgct
     1501 gctgctgcag gagtaatcct tccacctttc ttaaggtatt tcaaagtttc cagagtaata
     1561 tagatactgg aatccagctt ctgctcttcc agaacctgtt tgatctgtgt cgcatttctg
     1621 cccgcatctc ttaatgtgag ggcatccagt acagattgtc tctgtgtaac agaaattctc
     1681 tgattgtcta ctacatgtac tcttccgtca tattcctgag ccagtcccat tgctgtatcg
     1741 caggatgcac tcagtccgct tgacattgga atgtgcacta cttcatcata cttttccagt
     1801 aatgtattcc agagtccgca tacatctgcc ggccccggct gtgaagtaga aatagcctca
     1861 tcattcttaa gtctctcata aaactcttcc tgtgaaagag tgattccttc aagataaagc
     1921 ttctcgttaa tataaaaagg cattggaatt acgctgattc caagttctct tgcctggtcc
     1981 tgtgttatgc cgctgttact gtctgtgaca atggcaattt ttcccatatt catatctcct
     2041 ttgcttctga tccacttcag attttttatg tatcaattat cttttcataa aattacagac
     2101 tatgttcagt ttatcatatt tttttatgta tcacaatcag gaaccgctaa tttatcgcaa
     2161 aaaaatcccg catagattga cccatgcgga attttgactg cctgtttcta ttcttttgcg
     2221 atatttatgc cagaatcagt ccagttccct tacaatctta cgtaccggca gaacaaatgt
     2281 aggtgataca gataattcat caatccccat ctgtataaag cgttctgtca atgtagtatc
     2341 tgctccgagc tctccgcaga ttcctgccca gattccctct ttatgtgcat tatcaatgac
     2401 catctggatc atacgaagta cagcttcatg atgagaatca taaatattat ccagttttgc
     2461 attctgacgg tcgattgcca gtgtatactg tgttaaatcg ttagttccga tgctgaagaa
     2521 atcaacttct tttgcaagaa gatcactgat cataactgca gccggtgtct cgatcattac
     2581 accctgctct acgtcatctt taaacggaat gccctgctct gtcagctctt tcttaacctc
     2641 tgcaacaata gccttgatct gttttacttc tgttacagag atgatcatcg gatacataat
     2701 ggagatattt ccgaacatac ttgctctgaa cagtgcacga agctgtgtct taaatacttc
     2761 cggtctgtcc aggcagatac ggattgcacg ataacccatt gccggattct cctcgtgtgc
     2821 catatcaaag taatcaatct gcttgtctgc gccgatatcc agtgtacgga tgattacttt
     2881 ctttcctgcc atattctgtg ctactgcttt ataaatct
//
LOCUS       DBMDXG010000282         2917 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7562_length_2917_cov_1.890270, whole genome shotgun sequence.
ACCESSION   DBMDXG010000282 DBMDXG010000000
VERSION     DBMDXG010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7562_length_2917_cov_1.890270"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="ACZPD3_07875"
     CDS             complement(<1..257)
                     /locus_tag="ACZPD3_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR1415942.1"
                     /translation="MSERVILHSDMNCFYASVEMLHHPEFAGMPLAVGGDPEARHGIV
                     LTANYIAKQKGVKTGMALWQAKQICPEIIFVPPRMDLYLRFS"
     gene            452..1183
                     /locus_tag="ACZPD3_07880"
     CDS             452..1183
                     /locus_tag="ACZPD3_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415943.1"
                     /translation="MRDFGEILAENRKKKGYSQSDLVDLLSQEGIQVTTKAISKWETN
                     AREPALHVFLTLCQLLDIEDIYESFFGENPYNIMSGLNEEGRNKLIEFADILKASKKF
                     SPLSAKIIPFHHPVEITWEPVSAGTGNYLEDSVKETYDVGHLAPEQTDFGVRISGDSM
                     EPLYHTDDVAWIQKKDSLANGEIGIFYLNGNTYIKELHDEPDGVYLISLNQKYRPIQV
                     LESDSFKIFGKVIGKCKGAEIPGFH"
     gene            1240..1440
                     /locus_tag="ACZPD3_07885"
     CDS             1240..1440
                     /locus_tag="ACZPD3_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR1415944.1"
                     /translation="MAINNTLKDIREERNLIQADLAEAIGSCSQTIGRIERGERNPSL
                     EIAIRLAHYLKVPVEDIFQIED"
     gene            complement(1498..1887)
                     /locus_tag="ACZPD3_07890"
     CDS             complement(1498..1887)
                     /locus_tag="ACZPD3_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR1415945.1"
                     /translation="MKKEEIFEYVQKQYGTLPEYLWSKLPDSAVLRHKNGKWYAVIMT
                     VEKSKLGLDGKEPVDIMDVKCDPDMTNMIIQTYGFLPGYHMNKQHWITILLDGSVSEA
                     KVLDFLDMSYDLIDGAGRKEENVSGLQ"
     gene            2137..2460
                     /locus_tag="ACZPD3_07895"
     CDS             2137..2460
                     /locus_tag="ACZPD3_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HIR1415946.1"
                     /translation="MAERKRNKEIHFYVTEEERKLIRRKMIESKTKNMGAYLRKMAID
                     GYIVNTDTTPLKKQYEEMHKIGVNINQIAKKVNTTGDLYPEEMQELKEMVKEIWHILR
                     SSQSK"
     gene            2427..>2917
                     /locus_tag="ACZPD3_07900"
     CDS             2427..>2917
                     /locus_tag="ACZPD3_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIR1415947.1"
                     /translation="MAYIKIFPIKVTDKKALDYINNPDKTDEKLLISSFGCSPETADL
                     EFAMTREQTKKNGMDKGDNLAFHLIQSFKPGETDAEIAHRLGQQFANEVLKGKYEYVI
                     STHVDKNHIHNHIIFNAASFVDHHKYVSNKRSYHKICRISNRICQENGLSTSMPTGEK
                     GKS"
BASE COUNT          949 a          604 c          539 g          825 t
ORIGIN      
        1 gaaaatctca aatacaaatc catccgtggt ggtacaaata tgatttccgg acaaatctgt
       61 tttgcctgcc ataatgccat tccggtcttt acaccttttt gttttgcaat ataatttgct
      121 gtcagcacaa tcccatgtct ggcttccgga tcgccaccga ctgccagcgg cattccggca
      181 aattccggat gatgcagcat ttctacactg gcataaaaac agttcatatc actatgcagg
      241 atcacacgct cactcaaact tttcaccccc cagctgtttc cttcaacatg atgtcttatc
      301 aatttttgta tgtttagtat atatcgacat tccggagata gtcaagtaac atcttggttg
      361 tattttctcc attttggaga ttattgttga ttttatttct aacatacggt ataatgcata
      421 tagaaaattc aaatagaaat gaggcgaaca aatgcgtgat ttcggggaaa tattagcaga
      481 aaatagaaaa aagaaaggat actctcaatc agatcttgta gacctgcttt ctcaggaagg
      541 tattcaggtc actacaaaag caatctccaa atgggagacc aatgcacgag aaccagcttt
      601 acatgttttc ctgacacttt gccagttact tgatatcgaa gatatatatg aatccttctt
      661 tggagaaaac ccatataaca ttatgagtgg attgaatgaa gaaggcagaa ataaattgat
      721 tgaatttgct gatattttga aagcttctaa aaagttttct ccactgtctg caaaaattat
      781 cccattccat catcctgtag aaattacctg ggaaccagtt tctgctggta ctggaaatta
      841 cctggaggat tctgtaaaag aaacctatga tgtaggacac cttgctcctg agcagactga
      901 ctttggtgta cggatttccg gtgacagtat ggagccactt tatcatacag acgatgttgc
      961 atggattcag aaaaaagatt ctcttgctaa tggtgaaatt ggaatcttct atctcaacgg
     1021 caatacttac atcaaggaat tacacgatga gccagatggt gtttatctga tttctttaaa
     1081 tcaaaaatat cgtcctatcc aggttttgga atctgactct tttaagattt ttggaaaagt
     1141 aatcgggaaa tgcaaaggtg cagaaattcc gggatttcat tagatttcaa cagcaggttc
     1201 atgatcctgc tgataacaat aagcaaagga agtgattgaa tggcaatcaa taatacacta
     1261 aaagatattc gtgaagaacg gaatctcatt caagcagatt tggctgaagc cataggttcc
     1321 tgcagccaga ctataggccg catcgaacgt ggagaacgta atccctctct tgagattgca
     1381 atccggctgg cacattacct gaaagtgcct gtagaagata tttttcaaat tgaagactga
     1441 agcacaacag ccagaaaacc gaggagtaaa atccccggtt ttccttttgc tcaaaaatca
     1501 ctgtaatcca cttacatttt cttcttttct gcctgctccg tcaatcagat cataactcat
     1561 atctaaaaag tccagtacct ttgcttcact gactgaacca tccagtaaaa tcgtgatcca
     1621 gtgctgcttg ttcatatggt atccaggtaa aaatccataa gtttgaataa tcatatttgt
     1681 catatccgga tcacacttta cgtccatgat atcaaccggc tcttttccat ctaatcccag
     1741 tttcgatttt tcaaccgtca tgatcactgc ataccatttt ccatttttat gtcggagtac
     1801 tgcactgtcc ggtagtttac tccatagata ttccggaagt gtgccatact gtttctgcac
     1861 atattcaaat atttcttctt tcttcacaac tcattcaact cctgattctt ctaatatcct
     1921 gtccataatc ataccacatt tttgttccct atactacctg tcaaattctt ttgcattttc
     1981 ccggttctcc tgactttaac taatagagag gtaaaacagc aaggacagga agtgaggata
     2041 cacttccggg aaatcccaat ttagggtacc ctgccctaaa ttgaaaaacg ctgaaagcag
     2101 cgatactact gccctctcag aaaggagaac ccgccaatgg ctgaaagaaa aagaaacaaa
     2161 gaaattcatt tttatgtcac agaggaagaa cgaaagctga tccgcagaaa gatgatagaa
     2221 tccaagacca aaaacatggg agcctatctt cgtaaaatgg caatcgacgg ttacatcgtc
     2281 aacaccgata ccactccact gaagaaacag tatgaggaaa tgcacaagat tggcgtaaat
     2341 atcaaccaga ttgcgaagaa agtgaatacc accggagatc tgtatccgga agaaatgcaa
     2401 gaactgaaag agatggtaaa agagatatgg catatattaa gatcttccca atcaaagtaa
     2461 cggacaaaaa agctctggac tacattaaca atcctgataa gactgatgaa aaactattga
     2521 tttctagttt tggctgttct cctgaaacag ctgatctgga atttgccatg acgagagaac
     2581 agacaaaaaa gaacggaatg gataaaggtg ataatctggc atttcatctg attcagtcct
     2641 ttaagcctgg tgaaacagat gcagaaatcg cccatcgctt aggacaacaa tttgcaaacg
     2701 aagtactgaa aggaaaatat gaatatgtga tcagcaccca tgttgataaa aatcatatcc
     2761 ataaccacat catctttaat gcagcaagtt ttgttgatca tcacaagtat gtttccaata
     2821 agcggagcta ccataaaatc tgccggatca gtaatcgcat ctgccaggaa aatggacttt
     2881 ccactagtat gccaaccgga gaaaaaggaa aaagcta
//
LOCUS       DBMDXG010000283         2913 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7571_length_2913_cov_1.993599, whole genome shotgun sequence.
ACCESSION   DBMDXG010000283 DBMDXG010000000
VERSION     DBMDXG010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_2913_cov_1.993599"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="ACZPD3_07905"
     CDS             <1..1341
                     /locus_tag="ACZPD3_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012475.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIR1415948.1"
                     /translation="TPYREQYHFSPVVNWNNDPNGLCWFKGYYHLFYQLNPFGQEWNN
                     MYWGHAASKDLMHWTHLPVALEPQEEILNNLAIKGGAFSGSALPMGDKVYFYLTRHIG
                     PQDDGWDTVQYQTMTKSSDMIHFEPEKEIIREKPEGTNYDFRDPKAIKIGEKYYIVLG
                     ACVDEKGTFLLYESEDAENWKYRCPLITEETKIRTIECPDFFPLDDKYVAMGAWMSHY
                     DEYGRFQQCRYYVGDWNGDAMDVHTQQWVDFGSNCYAAQSFQHEDRRILIGWISDFYG
                     EHIATEPGAYGSMTLPRELHVKNEHVYTKPVEEVYTLLGDTVYEGTGREIKVGSIADN
                     RYYASVSFEETGDFNILLGQDGDKSISLTAEGGKVFFKMAGVKSDKVQFVSSVEKCRN
                     AEIFVDGRTIEVYLNDGEDVGTRLFYNSNRQGIFCLNSEKDAQVKICEMKSIWK"
     gene            1431..2798
                     /locus_tag="ACZPD3_07910"
     CDS             1431..2798
                     /locus_tag="ACZPD3_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1415949.1"
                     /translation="MTTSMIKGNPLKLMLQFAFPLLLGNLLQQTYNIIDAAIVGQILG
                     AKALASVGASSSIQFLVLGFCMGSCTGFGVPVAKYFGAEKIEKMRDYIFNGAVLCAGI
                     AVILTALCSVLCPQILHILSVPEDIYDNAYSYLLIIFLGIPFTILYNYLSSILRSVGD
                     SRTPFIFLALSAVLNIFLDLFCIVVLKLGCAGAAIATISAQAISGILCLIFIIRKMKL
                     LWLKKENRTIKGDAVKELLAMGMPTGLQFSITAIGSMVMQSANNGLGSTYVSAFTAAM
                     KIKQFTMCPFDAIATSASVFCSQNLGAGQSDRIKKGLRCGITVGVGYGIAAGILLIFA
                     GHTLSMLFVGKSAVAVLDASAKYLRCMGFFYWSLGILNVARMVTQGLGYSGRAVFSGV
                     TEMIARTVVCLGFVGTFGFTAICFADQTAWITATCYIFPTCLWCIRKSTKMLASIAAR
                     VNNAS"
BASE COUNT          862 a          507 c          704 g          840 t
ORIGIN      
        1 actccgtaca gagagcagta tcatttttca ccggttgtga actggaataa tgatcctaat
       61 ggactttgct ggtttaaggg ttattatcat cttttttatc agctgaatcc ttttggacag
      121 gagtggaata atatgtactg gggacatgca gcaagcaagg atctgatgca ctggacacat
      181 cttccggttg cgttagagcc tcaggaagaa atcctgaata atcttgcaat caaaggtggt
      241 gctttctccg gaagcgcact tccgatggga gacaaggtat acttctatct cacaagacat
      301 atcggacctc aggatgacgg atgggacact gttcagtatc agacaatgac aaagagtagc
      361 gatatgattc attttgagcc ggaaaaagag attatcaggg agaaaccgga aggtactaac
      421 tatgattttc gcgatccgaa agcaattaag atcggtgaga agtattatat tgtattaggt
      481 gcatgcgtag atgaaaaagg aacatttctt ctgtatgaat ctgaggatgc agagaactgg
      541 aagtacagat gtcctttgat cacagaggaa acaaagatca gaaccattga gtgtccggac
      601 tttttcccat tggatgataa atatgtagct atgggcgcat ggatgagcca ttatgatgaa
      661 tacggaagat tccagcagtg cagatattat gtgggagact ggaacggtga tgcaatggat
      721 gttcatacac agcagtgggt agactttgga agcaactgct atgcagcaca gagttttcag
      781 catgaagacc gcaggatcct gattggttgg atcagtgatt tctatggaga gcatattgct
      841 acagagccgg gagcttatgg aagcatgact ctgccaagag aacttcatgt gaagaatgaa
      901 catgtatata caaaacctgt agaggaagtt tatactcttc tgggggatac agtatatgaa
      961 ggcacaggcc gtgagatcaa agtcggctcc attgcagata acagatatta tgcatctgta
     1021 tcatttgaag aaacaggaga tttcaacatt cttcttggtc aggatgggga taaatccatc
     1081 agtctgactg cagaaggggg aaaagtattc tttaaaatgg ctggtgtgaa gagcgataag
     1141 gtacagtttg tttcttctgt ggagaaatgt agaaatgcag aaatttttgt agacggaaga
     1201 acaattgagg tatatttaaa tgatggagaa gatgttggaa ccagactatt ctacaatagt
     1261 aacagacagg ggatattctg cctgaacagt gaaaaagatg cgcaggtgaa aatttgtgaa
     1321 atgaaatcca tctggaaata aatcagagat ttcagaaatc ggaatcttag attttttcga
     1381 atatctgttt gacttatgga aacgttatag aaaatgaggg ataaatagaa atgaccacat
     1441 ctatgataaa gggcaatccg ctgaaactga tgttacagtt cgcgtttcct ttattattag
     1501 gaaacttgct gcaacagacc tataatatta ttgatgctgc gatcgtaggc cagattcttg
     1561 gcgcaaaagc actggcaagt gtaggagcca gcagcagtat tcagtttctg gtactgggat
     1621 tctgcatggg aagctgtaca ggctttggtg ttcctgtagc taaatatttc ggtgcagaga
     1681 aaatagaaaa aatgcgggat tatatcttta atggagctgt attatgtgca ggaatcgcag
     1741 tgatcctgac agcattatgc agtgttttat gtcctcagat tctgcatatt ttatctgtac
     1801 cagaggacat ttatgacaat gcatatagtt atctgctaat cattttcctg gggattccct
     1861 ttacgatttt atataactat ctgtccagta ttttgaggtc agtgggagac agcaggacac
     1921 cgtttatatt tctggcgtta tctgctgttc tgaatatttt tctggattta ttctgtatcg
     1981 tagtgctgaa acttggatgt gccggagctg ccattgcaac catttccgca caagcgatca
     2041 gtggaatctt gtgccttatt tttattatcc gcaaaatgaa acttctgtgg ctcaagaaag
     2101 aaaacaggac aataaaaggg gatgcagtaa aggaacttct ggcaatggga atgcctacgg
     2161 gacttcagtt ctccatcaca gctatcggaa gtatggttat gcagtctgcg aacaacgggc
     2221 ttggaagtac atatgtatca gcttttactg cagcaatgaa gatcaagcag tttaccatgt
     2281 gtccatttga tgctatcgca acctctgctt ctgtattctg cagccaaaat cttggagcag
     2341 gacagtctga caggattaag aaaggattgc gctgtggtat tacagtggga gtaggatatg
     2401 gtattgcggc aggaatttta ctgatttttg cagggcatac actttctatg ctgtttgttg
     2461 gaaagagtgc ggtggcagtt ctggatgcct ctgccaaata cctcagatgt atgggatttt
     2521 tctactggag tcttggtatt ctcaatgtcg cccgtatggt aactcaagga cttggatatt
     2581 caggcagagc cgttttctcc ggcgtaacag aaatgatcgc cagaacagtt gtctgtcttg
     2641 gatttgtagg aacttttggc ttcacagcca tctgcttcgc agaccagact gcctggatca
     2701 cagcaacctg ctatattttt ccgacatgcc tgtggtgcat ccgaaagtct acaaaaatgc
     2761 ttgcatctat agcagcccga gtaaataatg catcataatc cagcccatca gagtgtgaaa
     2821 ttctgcgtca gatatcttcc ttgtctttga actgtcttaa atgcattatt tattaggact
     2881 gctataaaaa agtatgacaa aataagaaaa cag
//
LOCUS       DBMDXG010000284         2908 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7582_length_2908_cov_1.966156, whole genome shotgun sequence.
ACCESSION   DBMDXG010000284 DBMDXG010000000
VERSION     DBMDXG010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7582_length_2908_cov_1.966156"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="ACZPD3_07915"
     CDS             complement(<1..1397)
                     /locus_tag="ACZPD3_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIR1415950.1"
                     /translation="MLKRKNDRQPPQRLEEKCYPNDYWLDEEDGGEDIPDLAVSSKPV
                     SGRLHSEPMVTGYEALERQRGVRRPNRKLTRREKKALKRAQKYEAKLHKEHEKADKRN
                     AKKEAKLAAKAEKQAMREAKKNAKKKKSSSSTAPPRQQPEGKKVSGGKGGSTEKKVAN
                     TAKDKRITAQQSIPYHEMGRDGICRVQDKFYSKTIRFYDINYQLAQNEDKNAIFENWC
                     DFLNYFDSTIHFQLSFINQHSNMAEYEKVIHINPQEDEFDDLRMEFAQMLNNQLAKGN
                     NGLMRTKYITFGIEAESIREARPKLERIESDIMNNFKILGVTAYPLNGTERLQIMYET
                     FNQDSKVPFHFSYDEVLRTGLSTKDYIAPTSFVFKNGKDFQMGGTIGAVSYLQILAPE
                     LTDKMLAEFLEMDSNLMVNFHIQSIDQMKAIKLVKSKVTDINRMKIEEQKKAVRAGYD
                     MDIIPSDLNTYGGEA"
     gene            complement(1375..1749)
                     /locus_tag="ACZPD3_07920"
     CDS             complement(1375..1749)
                     /locus_tag="ACZPD3_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HIR1415951.1"
                     /translation="MAFVSVPKDLTKVKNKIIFNLTKRQLICFGIAAAVGVPFYFFTR
                     GIIGSSNAATGMCLLMLPAFLFAMYEKDGLPLEKVLKNMITVKFIRPPVRKYQMENLY
                     EKKFIMVRKKKGGQKSVKKKKR"
     gene            complement(1767..2636)
                     /locus_tag="ACZPD3_07925"
     CDS             complement(1767..2636)
                     /locus_tag="ACZPD3_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HIR1415952.1"
                     /translation="MDRIFEAIEEWMRNLLTGMVSSNLTTMYTDVNEKTGQIAVQVGQ
                     TPQGWNGNIFSMIQNLSDSVIVPIAGMIITFVLCYELISMLTEKNNMHDIDTWMFFKY
                     FFKMWVAVWIVSHTFTITMAVFDVGQSVVSRAAGVISSDTAINIDTMITTMETAMESM
                     EIGELVILALETMLVSLCMKVISVFITVILYGRMIEIYLYSSVGAIPFATMSNREWGQ
                     IGNNYLRGLFALAFQGFFMMVCVGIYAVLVANIQMSDNIHSALFGVMAYTVIFCFSLM
                     KTGNFARSIFNAH"
     gene            complement(2660..2743)
                     /locus_tag="ACZPD3_07930"
                     /pseudo
     CDS             complement(2660..2743)
                     /locus_tag="ACZPD3_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
BASE COUNT          751 a          727 c          529 g          901 t
ORIGIN      
        1 ttggcttctc caccatatgt atttaagtct gatgggataa tgtccatgtc atatccggca
       61 cgaactgcct ttttctgctc ctcaatcttc atacggttaa tatcggtcac cttgctcttt
      121 accagcttga tcgctttcat ctggtcaatg gactggatat ggaaattcac cataaggtta
      181 ctgtccatct ccaaaaactc tgccagcatc ttgtccgtaa gctctggggc aagaatctga
      241 agataagaaa cggctcctat cgttccaccc atctgaaagt cttttccatt cttaaataca
      301 aagctggtcg gtgcgatata atcctttgtg ctaagacctg tccgaagtac ctcatcataa
      361 gaaaaatgga aagggacttt actatcctga ttaaatgtct catacatgat ctgcagcctt
      421 tccgttccat tcaacggata cgctgtcaca ccaagaatct tgaagttatt cataatatcc
      481 gactcgatac gttccagttt cggtcgtgct tcccggatac tctctgcttc gataccaaac
      541 gtgatatatt tggtacgcat cagaccatta tttccttttg ccagctgatt attaagcatc
      601 tgtgcaaact ccatacgcag atcatcaaac tcatcctcct gcggattgat atggataacc
      661 ttttcatact ccgccatgtt gctgtgctga ttgataaagg aaagctgaaa atgaatcgtg
      721 ctgtcaaaat aatttaaaaa atcacaccag ttttcaaaga tcgcattttt gtcctcattt
      781 tgagctaact ggtagttgat gtcataaaaa cggatggtct tggaataaaa tttatcctgc
      841 acacggcaga taccatctct ccccatctca tgataaggaa tggactgctg tgctgtaatc
      901 cgtttatctt tggcagtatt tgctactttc ttttcagtgc ttccgccttt tcctcccgaa
      961 accttctttc cttccggctg ctgtcttggc ggtgctgtag atgaactgct ctttttcttc
     1021 tttgcatttt tctttgcttc acgcatcgcc tgtttctctg cttttgccgc aagttttgct
     1081 tctttctttg catttctctt atccgctttt tcatgctctt tatgaagttt cgcttcatat
     1141 ttctgtgccc ttttcaaagc cttcttttcc ctgcgggtca gcttcctgtt tggtcggcgt
     1201 acccctctct gtctttccaa tgcttcatag cctgttacca ttggctcact gtgcagtctg
     1261 ccgcttaccg gcttactgct gaccgccaga tctggaatat cttctccacc atcctcttca
     1321 tccagccagt aatcatttgg ataacatttt tcttccaatc tctgcggtgg ctgtctatcg
     1381 ttttttcttt ttaacactct tctgtcctcc cttctttttt ctgaccatga taaatttctt
     1441 ctcatacaga ttttccatct gatacttccg taccggagga cggataaatt tcacggtgat
     1501 catatttttc aaaacctttt ctaacggaag tccatccttt tcatacatgg caaataaaaa
     1561 tgccggaagc atcaaaaggc acatcccggt ggctgcgtta ctggaaccga tgatccctct
     1621 ggtaaagaaa taaaacggaa ctcccactgc cgccgcaatc ccaaaacaga tcagctgccg
     1681 tttggtcagg ttgaagatga tcttattttt aaccttggtt aagtctttag gcactgatac
     1741 aaatgccatt gtcagttcct cctttcttag tgagcgttga agatactacg ggcaaagttt
     1801 cctgttttca tcaaactgaa acaaaagatc accgtgtatg ccatcacgcc gaacaatgca
     1861 gaatgtatgt tatctgacat ctggatattc gctactaaga ctgcgtaaat tcccacgcag
     1921 accatcataa agaatccctg aaatgccagt gcaaacaatc cacgaagata gttgtttcca
     1981 atctgccccc attcccggtt agacatagtt gcaaatggaa tagctccaac ggacgaatag
     2041 agatagattt caatcatacg cccatacaaa atcactgtaa taaagactga gataaccttc
     2101 atacacaggc tcacaagcat cgtttccagt gccagtatga caagttcacc gatttccata
     2161 gattccattg ccgtttccat cgtggtaatc atcgtatcta tattgatcgc tgtatcggaa
     2221 ctgatgacac cagcggcccg gctcactact gactgcccga catcaaatac cgccatggta
     2281 atcgtgaaag tatggctgac gatccagact gccacccaca tcttgaaaaa atatttaaag
     2341 aacatccatg tatcaatgtc atgcatattg ttcttttctg ttaacatgga aatcagctca
     2401 taacaaagca caaaggtaat aatcataccg gcaatcggta caatgaccga atcggaaaga
     2461 ttttgtatca tgctgaaaat gttcccattc cagccctgcg gtgtctgccc aacctgaaca
     2521 gcaatctgac cggtcttttc attgacatcg gtatacattg ttgtcaggtt ggaacttacc
     2581 atgcctgtca gaagattcct catccattct tcaattgctt caaatatccg atccatgagt
     2641 catctccctc ctaagtttct cagctaaaca aaccggaaag taacggtaca agctgggtgc
     2701 cgatcaaaac aacaccacct cccgccatca gctgttttat ccccaattag gtgtaaaaac
     2761 tctgctcgat ggcgggcggc ggcgtacaaa aaatctatat ggtgttgtta agagaaccgt
     2821 cccggcggga caggtcaggc agtgacctgt tccacctccg ggatgggttt gagattaaaa
     2881 tgaatttcga tagaaatgtg tcttgttt
//
LOCUS       DBMDXG010000285         2901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7597_length_2901_cov_2.246071, whole genome shotgun sequence.
ACCESSION   DBMDXG010000285 DBMDXG010000000
VERSION     DBMDXG010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7597_length_2901_cov_2.246071"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            3..173
                     /locus_tag="ACZPD3_07935"
     CDS             3..173
                     /locus_tag="ACZPD3_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012392.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1415953.1"
                     /translation="MKNTNKQVKTIAYESGFHNVDYFCRLFKKRYHLTPSEYRCTCLK
                     LLSADPSLQTHK"
     gene            complement(241..1839)
                     /gene="hcp"
                     /locus_tag="ACZPD3_07940"
                     /gene_synonym="priS"
     CDS             complement(241..1839)
                     /gene="hcp"
                     /locus_tag="ACZPD3_07940"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HIR1415954.1"
                     /translation="MDKKMFCYQCEQTVGCTGCTGNAGVCGKKAGTAKLQDELTGALI
                     GLARAVDSAAAISKSTSQIIIEGLFTTVTNVSFDDAAIENMIQKVRAEKERLVPGCSK
                     CQSPCGKSDEYDMQQLWNADEDIRSLKSLILFGIRGMAAYAYHANVLNYEDAEVNRFF
                     CEALFKIGYEESMDTLLPTVLKVGEINLKCMALLDKANTKTYGTPEPTAVTLTVEKGP
                     FIVVTGHDLKDLQLLLEQTEGKGINIYTHGEMLPAHAYPLLKKFSHLKGNFGTAWQNQ
                     QKEFDHLPAPILYTTNCLMPPKSSYADRVFTTEVVAFPGAVHIDEKKNFTPVIEKALE
                     LGGYKEDQTRTGINGGTKVTTGFGHAAILSHADTVVEAVKSGAIRHFFLVAGCDGAKP
                     GRNYYTEFVKQTPSDSIVLTLACGKFRFNDLELGEIGGLPRLMDMGQCNDAYGAIQVA
                     VALADAFGCSVNELPLSFVLSWYEQKAVCILLTLLHLGIKNIRLGPSLPAFLSPNILN
                     YLVENYGIAPITTPEEDIKALMNQ"
     gene            complement(1854..2573)
                     /locus_tag="ACZPD3_07945"
     CDS             complement(1854..2573)
                     /locus_tag="ACZPD3_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1415955.1"
                     /translation="MIRKIIRIDKEKCNGCGACADACHESAIDIINGKAELVREHFCD
                     GLGDCLPECPTGAISFEEREAPEYDEEAVKEAQKKIFAKNQAITAHAGCPGSKSMQIQ
                     RSEIPKSGKPSSTTGQVSNLQNWPVQIKLAPVSAPYFNGAKLLIAADCTAYAYAGFHQ
                     DFIRNKVTLIGCPKLDQVDYSEKLTAIIQNNDIQSVTIVRMEVPCCGGLEMATRKALQ
                     NSGKFIPWQVITIGIDGNIIE"
     gene            2670..>2901
                     /locus_tag="ACZPD3_07950"
     CDS             2670..>2901
                     /locus_tag="ACZPD3_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR1415956.1"
                     /translation="MKKYLPILKNSPFFTGLTDNEILSILHCVKATAVSQKRDSYIFR
                     AGDSTEVMGLVVSGCVLVIQEDLWGHRNILSKC"
BASE COUNT          760 a          636 c          596 g          909 t
ORIGIN      
        1 tattgaaaaa tacgaataaa caagtaaaaa cgatcgccta tgaaagtgga tttcataacg
       61 ttgattattt ttgcagattg tttaaaaaac gttatcattt aaccccatca gaatatcgct
      121 gcacatgttt aaaattgttg tcggcagatc cgagtcttca gacccataaa taaagccgat
      181 gcgggacttt ggaatcctgt atcggctttg ttaatttgcg aagattttga aaaatttatt
      241 ttactggttc attagtgctt tgatgtcttc ttccggtgtg gtgataggtg caataccata
      301 attttccacc agatagttca gaatattagg agacaggaac gccggaagag aaggaccaag
      361 acggatgttc ttgataccca ggtgtaaaag tgtcagcagg atacagactg ctttttgttc
      421 ataccaggag agaacgaagg aaagcggaag ctcatttaca gaacatccaa atgcatccgc
      481 aagtgccaca gcaacctgga ttgcaccata agcatcatta cactggccca tatccatcag
      541 tcgtggcaga ccaccgattt ctcccagctc aagatcattg aaacggaatt ttccacatgc
      601 cagggtgaga acgatactgt cagaaggtgt ctgtttcacg aattccgtat aatagtttcg
      661 acccggtttg gcaccatcac agccggcaac caggaagaaa tggcggatag cgcctgattt
      721 tactgcttct actacagtat ctgcatggga cagaatggct gcatgtccga aaccggttgt
      781 cactttcgta ccgccgttga tgcctgtgcg tgtctgatct tctttgtaac cacccagttc
      841 cagtgctttt tcaataaccg gtgtgaaatt tttcttctcg tcgatatgaa cagcgcctgg
      901 gaaagcaacc acttctgttg taaatactct gtcagcataa ctgctttttg gcggcatcag
      961 acagttggtg gtgtaaagga tcggagccgg aagatgatca aactctttct gctgattctg
     1021 ccatgcagtt ccgaaatttc cttttaagtg agagaatttc tttaagagtg gataggcatg
     1081 ggcagggagc atttcaccgt gggtatagat gttgattcct tttccttctg tctgttcaag
     1141 taaaagctgc agatccttca gatcatgacc ggttactaca ataaatggtc ccttttctac
     1201 ggtaagtgta actgcagtcg gttccggtgt tccataggtt ttggtatttg ccttgtcaag
     1261 aagtgccata catttcagat tgatttcccc tactttcagt acagtaggaa gcagtgtatc
     1321 catactttct tcataaccaa tcttaaataa tgcttcgcag aagaaacggt ttacttcagc
     1381 atcttcataa ttcagaacat ttgcatgata agcataggct gccattccgc gaatcccgaa
     1441 aagaataagg gacttcaggg agcggatatc ttcatctgca ttccacagct gttgcatgtc
     1501 atattcgtct gatttaccgc aaggtgactg acatttactg cagccaggaa ccagtctttc
     1561 tttttctgct ctgacttttt gtatcatatt ttcgattgct gcatcgtcaa aactcacatt
     1621 tgtaacggta gtaaacagtc cttcaattat gatctgactg gtacttttgg aaatagctgc
     1681 tgcgctatcg acagcccttg ccagaccgat caacgcacct gtcagctcat cctgtaattt
     1741 ggcagtgcct gccttttttc cgcagacacc tgcatttcct gtgcagccgg tgcatcccac
     1801 tgtctgttca cactgataac aaaacatttt tttatccatt gtcgtatcct cctttattct
     1861 ataatatttc catcgatacc tatggttata acctgccagg gaatgaattt tccactattc
     1921 tgaagtgctt ttctggttgc catctcaagt ccgccacagc atggaacttc cattcttaca
     1981 atcgtcacac tttgaatatc gttattttgg attattgcag tcagtttctc gctataatcc
     2041 acctgatcca atttaggaca gccgattaat gttaccttat ttcggataaa atcctgatga
     2101 aagcctgcgt aagcgtaagc agtacaatcg gctgcgatta aaagctttgc gccgttaaaa
     2161 tatggggcac tgacaggtgc cagcttaatc tgtaccggcc agttctgaag attggatacc
     2221 tgacctgtgg ttgaagatgg ttttcctgat ttgggtattt ctgatcgctg aatctgcatg
     2281 cttttagacc cgggacagcc ggcatgagca gttattgcct gatttttggc aaatattttt
     2341 ttctgtgctt ctttcacagc ctcttcatca tattcaggtg cttcccgttc ctcaaaggaa
     2401 atggcacctg tgggacactc cgggaggcaa tcccccagtc cgtcacagaa atgttctctc
     2461 accaattctg cttttccatt aatgatatca atggcacttt cgtggcaggc atctgcacag
     2521 gcgccgcagc cattgcattt ttctttatcg attcgaataa tctttcttat cattttacct
     2581 ccgtcaatag atttaacttt tgtttctgtc ggcattatag tatgataaat acaacttgta
     2641 tgtttgattt ccaacaagga ggaatgattt tgaaaaaata tttacccatt ttaaaaaaca
     2701 gtccattctt tacaggcctt actgataacg agatattatc tatactgcat tgtgtaaaag
     2761 caacagcagt ttcccaaaaa cgagattctt atattttcag agcaggagat tctactgagg
     2821 taatgggact tgtggtgtcc ggctgtgttc ttgttattca ggaagacctt tgggggcatc
     2881 ggaatattct gtcgaaatgt c
//
LOCUS       DBMDXG010000286         2894 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7628_length_2894_cov_1.383101, whole genome shotgun sequence.
ACCESSION   DBMDXG010000286 DBMDXG010000000
VERSION     DBMDXG010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7628_length_2894_cov_1.383101"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="ACZPD3_07955"
     CDS             <1..394
                     /locus_tag="ACZPD3_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415957.1"
                     /translation="VYQKLLEREDLSEQRKGFAASVRYNLGCAYSYLFQMEKAQECFL
                     EAYREAHSKDALKAYIIAYSSVHDKTDYDKVMEELKVDEELKKDIKEEIRQSLKAFES
                     VPEEKTDEKNLDALLERLMKDYHRSTGS"
     gene            502..1398
                     /locus_tag="ACZPD3_07960"
     CDS             502..1398
                     /locus_tag="ACZPD3_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR1415958.1"
                     /translation="MKKRSLAVLMAGVMAASMMTGVVSVYADADSKTLTVGFDAQYPP
                     FGYKDDNGEYVGFDLDLAQEVCDNLGWELVKKPINWDSKDMELNSGSIDCIWNGFTIN
                     GREDDYTWSDPYLNNEQVMVVAADSGIEKLDDLAGKNVVVQAASAALDALNSDDNKDL
                     TASFASLTENPDYNTAFMNLDSGAADAIAVDIGVAKYQLSQREEGKYVILDEPIQSEE
                     YGIGFKKGNDELKDTVWEEVLKLYDAGEVDKLAEKYEVADMLCIGDDEKSDDKKDDAA
                     EDDASDADTAKEDTADTAKDAE"
     gene            1534..2184
                     /locus_tag="ACZPD3_07965"
     CDS             1534..2184
                     /locus_tag="ACZPD3_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR1415959.1"
                     /translation="MSLSVMLQQLASGMVVSVEIFVVTLLFSLPLGLLICFGRMSKNP
                     AIRGIVSAYISVMRGTPLMLQLMVVYFGPYFIFGIRISMGYSLIAVFIAFSINYAAYF
                     AEIYRGGIESISAGQYEAAKILGYSKAQTFFRIILPQVIKRILPSVTNEVITLVKDTS
                     LAFVVAVSEMFTIAKQIASAQTTMMPFVIAAVFYFVFNLLVAIVMDKIEKKLNYYR"
     gene            2251..>2894
                     /locus_tag="ACZPD3_07970"
     CDS             2251..>2894
                     /locus_tag="ACZPD3_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR1415960.1"
                     /translation="MVLFEMKNIKKSFGSLEVLKDISLQVEEGEVLSIIGPSGSGKST
                     LLRCATGLETPDSGEIIKQGDVGLVFQNFNLFPHFSVLKNITDAPIRVQKRKKEEVYA
                     QARTLLKQMGLADRENAYPFQLSGGQQQRVSIARALCMNPKILFFDEPTSALDPELTS
                     EILKVIKDLAAEHITMVIVTHEMTFARDISDHIIFMDKGLIAVEGSPKEVFESE"
BASE COUNT          885 a          496 c          711 g          802 t
ORIGIN      
        1 ggtataccag aaactcctgg aaagagagga tctgtctgag cagagaaaag gattcgcagc
       61 ttcagtgcgc tataatcttg gctgtgcata cagttatctt tttcagatgg aaaaagcaca
      121 ggaatgtttt ctggaagcat acagagaagc acattcaaaa gatgccttaa aagcttatat
      181 catagcatac agcagtgttc atgataagac agattatgat aaggttatgg aagaacttaa
      241 agtagatgaa gaactgaaga aagatataaa agaagaaatc aggcagtctc tgaaagcttt
      301 cgagtctgta ccggaagaga aaacagatga aaaaaatctg gatgcccttc tggagagact
      361 tatgaaagat tatcacagaa gtacaggttc ataacttact cttgacagag gcggaggtgt
      421 gtgataggat taacacatac tttcacatgg tagagtggct gtgtgataaa taacaaaaca
      481 gataaagagg agatatttat catgaaaaag agatctttag cagtactcat ggcaggagtt
      541 atggcagctt ctatgatgac gggtgttgtt tctgtatatg cagatgctga cagtaagaca
      601 ttaactgtag gatttgatgc acagtatcct cctttcggat ataaagacga taatggagag
      661 tatgttggtt ttgaccttga ccttgcacag gaagtatgcg acaatctcgg atgggaactt
      721 gtaaagaaac cgatcaactg ggattccaag gatatggaac tgaattccgg ttctattgac
      781 tgtatctgga acggatttac cattaacgga cgtgaggatg actatacatg gtcagatcca
      841 tatctgaaca atgagcaggt gatggttgtt gcagccgatt cagggattga gaagttggat
      901 gacctggcag gcaagaatgt agtagttcag gccgcttccg cagcactgga tgcattaaac
      961 agcgatgata ataaggatct tacagcaagc tttgcatcac tgacagagaa tcctgattac
     1021 aatacagcat tcatgaatct ggattccggc gctgcagatg ccatcgcagt agatatcggt
     1081 gttgccaaat atcagctttc ccagagagaa gaaggcaaat atgtgatcct tgacgagcca
     1141 atccagagcg aagagtatgg aatcggcttt aagaagggta acgatgagct gaaagatact
     1201 gtatgggaag aagttctgaa actttatgat gcaggtgaag ttgataaact tgctgagaag
     1261 tatgaagtag cagatatgct ctgcattgga gatgatgaga aatctgatga taaaaaagat
     1321 gatgcagcag aggatgatgc ttccgatgca gacacagcaa aagaagatac agctgataca
     1381 gcgaaagatg ctgaatagtc agccggaaca aaaacagtta tgatacaaga ggacagatct
     1441 gtgcaggctg tccgcattat aaaggaatgg agggtacatt ttgtgcaccc tcttttctta
     1501 ctgtacagga gaaatttttc aggaggaaag cttatgtctt taagtgtaat gctgcagcag
     1561 cttgccagtg gtatggtggt ttctgtagag atttttgtag tgacattact tttttcactt
     1621 ccgctgggac ttctgatctg ctttggcaga atgtctaaga atcctgcgat acgtggaatt
     1681 gtgtctgcct acatatcagt tatgcgcgga accccgttaa tgctgcaatt gatggttgtg
     1741 tatttcggac catactttat ttttggaatc cgtatttcca tgggatacag cctgatcgct
     1801 gtgtttatcg cattcagtat taactatgct gcatattttg cggagattta cagaggagga
     1861 atcgaatcta tctcagcggg acagtatgag gctgcaaaga ttctgggata tagcaaggcc
     1921 cagacatttt tcaggatcat cctgccgcaa gtgatcaaga gaatccttcc atctgtgaca
     1981 aatgaggtta tcacactggt aaaggatacg tcactggcat ttgttgttgc tgtttcggag
     2041 atgtttacaa ttgcaaaaca gattgcaagt gcacagacga ctatgatgcc ttttgtgatc
     2101 gctgctgttt tctattttgt gttcaatctt cttgttgcga ttgtaatgga taagattgag
     2161 aaaaaactga actattatcg ctgacaggcg gctggggtta taaaatattt tttgactctg
     2221 acatcattta atttaagagg aatatttgct atggtattat ttgaaatgaa aaacataaag
     2281 aagagcttcg gcagccttga agtattaaaa gacatttcac ttcaggtaga ggagggtgag
     2341 gtccttagta ttatcggacc ttccggttcc ggtaaatcca cacttcttcg ctgtgccacc
     2401 ggtcttgaga caccggacag cggtgagatc atcaagcaag gggatgtggg actggtattt
     2461 cagaacttta atcttttccc tcatttttcc gtactgaaaa acatcacaga tgctccgatc
     2521 agagtgcaga agagaaagaa agaagaggtt tatgcacagg caagaactct gttaaagcag
     2581 atgggacttg cagacagaga gaatgcatat ccgtttcagc tttccggcgg tcagcagcaa
     2641 cgtgtctcta ttgccagggc attatgtatg aatccgaaga tcctgttttt tgatgagcct
     2701 acttccgccc ttgatccgga gcttacgagt gaaattctta aggtaatcaa agatctggca
     2761 gcagaacaca tcactatggt gatcgttact catgagatga catttgcaag agatatctct
     2821 gatcatatta tttttatgga taagggactg atcgcagttg aggggagtcc aaaagaggta
     2881 tttgaatcag aaca
//
LOCUS       DBMDXG010000287         2892 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7635_length_2892_cov_1.903977, whole genome shotgun sequence.
ACCESSION   DBMDXG010000287 DBMDXG010000000
VERSION     DBMDXG010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7635_length_2892_cov_1.903977"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="ACZPD3_07975"
     CDS             <1..813
                     /locus_tag="ACZPD3_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005422249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415961.1"
                     /translation="MIVPMVNAIYLSFTSSTALSVGFNSKFVFLDNYKYMFQDKDFLQ
                     ALFNTLKLMLVIPAVTIFLSLVFAFILTQGQLKEKSFYRTIFFFPSIVSMTVVGIVWS
                     FVFNPTRGILNHMLDLFHLSTWKHAWLGEGKTALWCIGAALVWQAIGYYMVMHVASID
                     GISQEVYEAASIDGATGVQKFFRITIPLLRRSIGTTYILSLSGTINLSFTLSNVMTGG
                     GPNGASSVLLQYMYTQGMRNANFGYAMAIAMFTLILAIVLAMISKKVSSEKE"
     gene            825..1652
                     /locus_tag="ACZPD3_07980"
     CDS             825..1652
                     /locus_tag="ACZPD3_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1415962.1"
                     /translation="MKTVRTWCIRILMILFTVIFLYPLFWNLMSAFKTNAEYLTDPYA
                     LPTALNLDNFVAAWQKANIAAYFGNSIFVTVFSTVLLLLLVIPISYVLARYRFAGSKL
                     ISAIYMACIFLQATYIMIPLFLELQAVNGLNNLPVLCLVYAVMQFPFCIFTLQGFMSA
                     VPRDYEESARIDGATNIQLLSRVMVPLAKPGIATITMLSAMGFWNEYPLALVLLTEDA
                     KKTLPIGMANLFEVQKYATDWGALFAGLVIIMIPTIIIYLIGQRYLLQGIGAGGLKG"
     gene            1671..>2892
                     /locus_tag="ACZPD3_07985"
     CDS             1671..>2892
                     /locus_tag="ACZPD3_07985"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR1415963.1"
                     /translation="MPTDQELIKIVPSSRQLAYQATEFYAFFHFGMNTYTNREWGDGT
                     ETPQIFNPTEFIADQWVSAAQNAGMKGVILTCKHHDGFCLWPTRYTSHSVVSSPWKNG
                     RGDVVWEVSEACRRYGMKFGIYLSPWDRNKPCYGSGKEYDDYYLAQLTELLTGYGDIF
                     SVWLDGACGEGPNGKKQIYDWKRYYECVRKYQPDACICVCGPDIRWCGNEAGDVRKSE
                     WSVVPARTALAESVQERSQQTDDKEFRMRRITSDMEDLGSRRALEGETNLIWYPAEVN
                     TSIRPGWFYHPEEDDQVKSLEELIYIYIGAVGGNATFLLNIPPMPNGLLHENDVKRLE
                     EFGSWKKKSFTHNLMSTAHIFSENEDPTHPVSDLTDDTSETWFQPESSELPVEITICL
                     DGSYNLGYLVLKEAV"
BASE COUNT          803 a          537 c          672 g          880 t
ORIGIN      
        1 atgattgtcc ctatggtaaa tgccatttat ctgtctttta ccagttccac tgcacttagc
       61 gtaggcttta acagcaaatt tgtatttttg gataattaca agtatatgtt ccaggataag
      121 gattttcttc aggcactgtt taatactctt aagctgatgc tggtaatccc ggctgtgacc
      181 atatttctaa gtcttgtatt tgcatttatc cttactcagg gacagctcaa ggaaaagtca
      241 ttttaccgga ccatattctt tttcccaagt attgtttcca tgactgtagt tggtattgtg
      301 tggtcttttg tgtttaatcc cacaagagga attctgaatc atatgctgga tctgttccat
      361 ctctccactt ggaaacacgc atggcttgga gagggaaaaa cggctctctg gtgtatcggt
      421 gcagccctgg tgtggcaggc tattggttat tatatggtta tgcatgtggc ttccattgac
      481 gggatttccc aggaggttta tgaagctgct tccattgacg gtgcaacagg agtgcagaag
      541 ttctttagga ttaccattcc tctgcttcgc agatccattg gaacgaccta tatactttcc
      601 ctgtctggta ctatcaattt aagttttact ctgtccaacg ttatgacagg cggtggacca
      661 aacggtgctt ccagtgtgct tttacagtat atgtatacgc agggtatgcg aaacgctaat
      721 tttggatatg ccatggcaat cgccatgttt accctgattc ttgcaattgt gcttgcaatg
      781 atttctaaaa aagtatcatc tgaaaaagaa taggagggag actgatgaaa actgtaagaa
      841 cctggtgtat cagaatcctg atgattctgt ttaccgttat ctttttgtac ccgcttttct
      901 ggaacctgat gtctgcgttt aagacaaatg cagaatattt gacagatccc tatgcattgc
      961 ccacagcttt gaatttggat aattttgtgg cagcatggca gaaagcaaat atcgccgctt
     1021 attttggaaa ttccatattt gtgactgtgt tttccactgt attgctattg cttctggtta
     1081 ttcccatttc ttatgtactg gcaagatacc gttttgcggg cagcaaactg atctctgcca
     1141 tttatatggc atgtattttt ctgcaggcta cctacattat gattcctttg tttctggaat
     1201 tacaggctgt gaatggatta aataacctgc cggtactatg ccttgtttat gcagttatgc
     1261 agtttccttt ttgtatcttt actttacagg gctttatgtc tgcagtaccc agagattacg
     1321 aggaatctgc aagaattgac ggtgctacta atatccaact gctatcaaga gttatggtgc
     1381 ctcttgcaaa acccggaatt gcgaccatta cgatgttaag tgccatgggc ttctggaatg
     1441 aatatccatt ggcacttgtt cttctcacgg aagatgcaaa gaaaactctt cccatcggaa
     1501 tggcaaactt gtttgaagtg cagaaatatg ccacggactg gggtgcactg tttgcaggac
     1561 ttgtaattat tatgattcca acaatcatta tttatctgat tggacaaaga tatctgcttc
     1621 agggaattgg tgcaggcggg ttaaaagggt aaaatgaaag gggaaataaa atgccaactg
     1681 atcaggaatt aataaaaata gttccctcca gccgccagct tgcctatcag gcaacagagt
     1741 tttatgcgtt ctttcatttt ggaatgaata catataccaa tcgtgagtgg ggagatggaa
     1801 cagaaacacc acaaattttt aatcctacag agtttattgc agaccagtgg gtgtctgcag
     1861 cacaaaatgc aggtatgaaa ggggtaatcc ttacctgtaa acaccatgat ggtttctgcc
     1921 tgtggccgac cagatatacc agccatagcg ttgtctccag tccatggaaa aatggaaggg
     1981 gagatgtggt ctgggaagtg tctgaagcat gccgtcgtta tgggatgaaa tttggaattt
     2041 atctttcacc atgggatcgc aacaagcctt gttacggaag cggaaaagaa tatgatgatt
     2101 actatctggc acagctgact gagcttctca ccggttatgg agatattttt agtgtctggc
     2161 tagatggtgc ctgcggagaa ggaccaaatg gaaaaaaaca gatatatgac tggaaacgct
     2221 attatgaatg tgtcagaaaa tatcagccag acgcatgtat ctgtgtgtgt gggccggata
     2281 tcaggtggtg cggaaatgaa gctggcgatg taagaaaatc tgagtggagt gttgttcctg
     2341 cacgtacagc acttgcagag agtgtacagg aaagaagcca gcagaccgat gataaggaat
     2401 ttagaatgcg ccggatcacc agcgacatgg aagatcttgg aagcagaaga gcgcttgaag
     2461 gtgaaacgaa tctgatctgg tatccggcag aagtaaatac ttctataaga cctggatggt
     2521 tttatcatcc agaagaagat gatcaggtga aaagtctgga ggagctgatc tatatctata
     2581 ttggggctgt tggaggaaat gcaacctttt tgttaaatat tccacctatg ccaaacgggc
     2641 ttcttcatga aaatgatgtt aaacgtctgg aagaatttgg aagttggaaa aagaaatctt
     2701 ttactcataa tttaatgagt acagcacata ttttttcaga aaatgaagat ccgacgcatc
     2761 cggtttctga tttgacagat gatacttcag aaacctggtt tcagcctgaa agcagcgaac
     2821 tgccagtaga aataaccatt tgtctggacg gttcatacaa cttagggtat ctggtattaa
     2881 aagaggctgt ct
//
LOCUS       DBMDXG010000288         2882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7658_length_2882_cov_1.611291, whole genome shotgun sequence.
ACCESSION   DBMDXG010000288 DBMDXG010000000
VERSION     DBMDXG010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7658_length_2882_cov_1.611291"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            734..1378
                     /locus_tag="ACZPD3_07990"
     CDS             734..1378
                     /locus_tag="ACZPD3_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR1415964.1"
                     /translation="MNLENMTKIYARGDSRIQLKVIPGHFVTSQSHITHYLDLTTMKS
                     RTAEAQNIAHELAANYEVSTPVDTIICMDGLEVIGAYLSEELTKAGIFSLNAHQTIYV
                     ITPESSNSGQIIFRDNFQPMIKGKNVLILNGSITTGSTLSKAIESILYYGGTIRGIAA
                     IFSRVDSVASLPVYSIFNTKDIPDYHSYSSTKCPMCQRQQKIDAIVSSYGYSAL"
     gene            1442..1688
                     /locus_tag="ACZPD3_07995"
                     /pseudo
     CDS             1442..1688
                     /locus_tag="ACZPD3_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391896.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
     gene            1716..1805
                     /locus_tag="ACZPD3_08000"
     CDS             1716..1805
                     /locus_tag="ACZPD3_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013406.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415965.1"
                     /translation="MAIPNLICMLLLSGEIAKDVKEFQPEIKK"
     gene            complement(1923..>2882)
                     /locus_tag="ACZPD3_08005"
     CDS             complement(1923..>2882)
                     /locus_tag="ACZPD3_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR1415966.1"
                     /translation="GWYPGITPDNKFLPDKQCYAHAFVLLAASSALLAGEKDAETLLK
                     DALELFDKRFWDEKQGLTYDTWNTEFTVLDDYRGLNANMHTVEAFLAVADAIKEEKYR
                     IRAGRIIDHVIGWASANDWRIPEHFTKEWKADLDCNKECPADRFKPYGATPGHGIEWA
                     RLIVQWAYSSYKDTPDSAEVYLKAAEKLYNRAVEDSWNVDGNPGIVYTTDWDGKPVVH
                     DRMHWTLAEAINTSAVLWHITHKQKYAKDYEEFMRYLDDKVLDYKNGSWFHQLDENNN
                     VIGTVWPGKSDVYHAFQSTWIPYGTPYISIASDVKQYMEVCHQ"
BASE COUNT          810 a          660 c          484 g          928 t
ORIGIN      
        1 ttcggatgat gagggaggtg tgtaaagtgg ctaatattca atgtctttat catcatcaca
       61 gaccagaata ttagccactt tacacacctc cctcatcatc cgaaatgcct gtctgaaatc
      121 tctcatgtac agacatcccg gcttcttatt ttgtatttct gatattagaa taatcgaagc
      181 ttcttaatat gaaagggaca aattcttaag attttcttat gattattcac ttttcgcagt
      241 caaaatatca ggctgttaat ctgtcacttt tatcaggctc ctattttcat ccaattcttc
      301 tattcgttct gctatataca ttttttatct catcttttct ctttttctga cagtcaataa
      361 tagactgaaa atcattatat catttcacat atattacttc tatagaatat tctcttctgt
      421 ttcccatttc cacacccatc actgtttatt ccattctcca taactcacaa ccaatctata
      481 gcaaccctcg taaataatac ttccatcaac gcaccagggg atcatcgtta tatgtttttt
      541 ggtattttat taacaatctt catacatatc gcatattttt ttattgattt tgtacaatat
      601 tatttatttt ttggcaaaac aatgctacaa tgttttataa gattagtgta ctgatttata
      661 actgttcaat ggtctgctaa ccgcaaagct actgaacagt tacacgtttt ttttcaacga
      721 gggaggtatt cttatgaatc tggaaaacat gacaaaaatc tacgcccgtg gtgacagccg
      781 cattcagctg aaggtaattc ccggacattt cgtcacatct cagtcacata tcactcacta
      841 tctggatctt acaactatga aatccagaac tgccgaagca cagaatattg ctcacgaact
      901 ggctgctaat tatgaagtat ctactcctgt tgataccatt atctgtatgg acggactgga
      961 agtaattggc gcatatcttt ctgaagaact taccaaagcc ggcatttttt ccctgaatgc
     1021 tcatcagacg atctacgtga ttactcctga atccagcaat tccggacaga ttatcttccg
     1081 tgataatttt cagccaatga tcaagggcaa aaatgtactg atcctcaatg gttcgattac
     1141 cacaggctct actttgtcca aagcaatcga gagcattctg tattacggag gcactatcag
     1201 aggtatcgca gccatattca gcagagtaga cagcgtggct tctctccctg tatattctat
     1261 ttttaacacc aaagatatac cggactatca ttcctattca tctacaaaat gtccaatgtg
     1321 tcagaggcag cagaaaatcg atgccattgt cagcagctac ggatattcag ctctgtaaat
     1381 cttcgaagta ttttataaag aaattgttac cagaattgat gactttgtct ggggaccggt
     1441 gatgctgatc ctgttggtcg gaaccggtat cttcctgact gtgcgcacca gatttcttac
     1501 atggcgtaac cttggctatg cactgaaaag cacactcagc aaggaagccc ggcaccaaga
     1561 gccgcggaca gggagatgta tcaccttttt ccgcactgac aaccgcactg gcagccacca
     1621 tcggtaccgg aactatcgtc ggtgttgcca cagccatggt ttccggtggt cccggagctc
     1681 tggtatggaa cttctccgat atcaccaacg cactgatggc tatcccgaac ctgatctgca
     1741 tgctcctgtt aagcggcgag attgccaaag atgtaaaaga attccagccg gagattaaaa
     1801 aatagttacc cacatcattc tttttatcat cctgaagtaa aatgcagcca gaaccttgtt
     1861 ccgtcaggtt ctggctgcat tttacagaaa tcttgtacgc cgggtacaaa taaacactta
     1921 ctttattgat ggcatacttc catatactgt tttacatctg aagcaattga tatatatggt
     1981 gttccatatg ggatccaggt tgactgaaaa gcatgatata catctgattt tcccggccat
     2041 actgttccta tcacattatt attttcatct aactgatgaa accaggaacc atttttataa
     2101 tccaatacct tatcatccag gtatctcata aactcttcat aatcttttgc atatttctgc
     2161 ttatgagtta tatgccataa gactgctgat gtattgattg cctctgccaa tgtccaatgc
     2221 attctgtcat gaacaacagg ttttccatcc caatcagtcg tatacacaat tccaggattt
     2281 ccatccacat tccaggaatc ttcaactgct ctgttgtaga gtttctctgc agcttttaaa
     2341 tatacttctg cagaatcagg ggtatcttta taagaagaat atgcccactg cacgatcaat
     2401 ctggcccact caattccatg gcccggtgtt gctccataag gtttaaaacg atcagcagga
     2461 cattctttat tgcaatcaag atcagctttc cattcctttg taaaatgttc aggtattctc
     2521 cagtcatttg cgcttgccca tccaataaca tgatctataa ttcttcctgc acgtattctg
     2581 tacttttctt cttttattgc atctgctact gccagaaacg cttctactgt atgcatattt
     2641 gcgttaagac cgcgataatc atcaagaact gtaaattctg tattccaggt atcataagta
     2701 agtccctgtt tttcatccca gaaccgttta tcaaataatt ccaatgcatc tttaagcaga
     2761 gtttctgcat ctttttctcc tgccagcaaa gcagaagaag cagccagaag aacaaatgca
     2821 tgagcataac actgtttatc tggcaagaat ttattatctg gtgtaattcc cggataccat
     2881 cc
//
LOCUS       DBMDXG010000289         2870 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7696_length_2870_cov_1.570603, whole genome shotgun sequence.
ACCESSION   DBMDXG010000289 DBMDXG010000000
VERSION     DBMDXG010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7696_length_2870_cov_1.570603"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            114..2168
                     /gene="recG"
                     /locus_tag="ACZPD3_08010"
     CDS             114..2168
                     /gene="recG"
                     /locus_tag="ACZPD3_08010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR1415967.1"
                     /translation="MSTSLRTLKGVGEKTEKLFAKIGVTDMESLLSYYPRNYDAYEEP
                     VEIRSLEEGAVVAISVAVITGVYVNQVRNLQVITTTVADLTGKISVTWFNAPYLRSAV
                     RKGSRFVLRGRVVRKQGKLQMEHPEIFTPAAYEEILHSLQPIYGLTAGLSNKTIVKLI
                     HQVLDEQKLQTEYLADEYKERYHLADRNFAIPAIHFPKNMQELLAARRRLVFDEFLLF
                     ILAVQSLKEKTEEAPNAFPMHPVWTTEQIIESLPYDLTKAQLNVWHEIERDLSGQALM
                     SRLVQGDVGSGKTILAFLAMIMTVENGYQAVLMAPTEVLARQHFQAMEKLLQEQNIDF
                     GHPVLLTGSDTAKEKREKYVLIASKEANLVIGTHALIQEKVQYNNLGLVITDEQHRFG
                     VKQREALTTMGNPPNVLVMSATPIPRTLAIIIYGDLDISVIDELPAQRLPIKNCVVDT
                     SYRPKAYSFMEKQIRQGRQVYVICPMVEESEGMDGENVLDYTLKLRNVFSPDIKIASL
                     HGKMKAKEKNVIMEAFAAGEIQILVSTTVVEVGVNVPNATVMMVENAERFGLAQLHQL
                     RGRVGRGEYQSYCIFMQGNGAKEISKRLQILNKSNDGFYIAGEDLKLRGPGDLFGIRQ
                     SGLLEFKLGDIYQDADILKAASETASEILSLDADLSLPQNEELQRRLSAYMKEDLQNL
                     GL"
     gene            2392..>2870
                     /locus_tag="ACZPD3_08015"
     CDS             2392..>2870
                     /locus_tag="ACZPD3_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415968.1"
                     /translation="MATKATTADLNTSTAKTTEVKEAPAKTTAEEVAVNKEANKKETA
                     ETETVKKETTAKKPAARRTRKTAEKKSAEAEKETEKKETAKAADTVEKKPAETEKEAE
                     KKETAKTTKTAAKPTKTTKRTTKKAPAKTAEKPAEETAAEKKTTRRTTKKEATAEVY"
BASE COUNT          913 a          497 c          768 g          692 t
ORIGIN      
        1 tatccaattg ctacctacat tctaacagct taagcaacaa gatggataat tccttactgg
       61 ctgtaaggga tattaaccat aaatatattg tagtagaaag gaatcggtgg tatatgtcaa
      121 catctttgcg tactttgaag ggcgtggggg agaaaacgga aaagttattt gcgaaaatag
      181 gagttacgga tatggaatct cttctgagtt attatccacg taattatgat gcgtatgagg
      241 agcctgttga gatccggtct ctggaagagg gagctgttgt ggcgatttct gtggcggtta
      301 taacgggtgt gtatgtgaat caggtgcgga atcttcaggt gatcacgacc actgtggcag
      361 atcttacggg gaaaatttct gttacgtggt ttaatgcacc gtatctgcgt tctgcagtca
      421 ggaaaggcag caggtttgtt ctgcgggggc gtgtcgtcag gaagcagggg aagctgcaga
      481 tggagcaccc tgagattttt acgcctgcgg cttatgagga gattctgcac agtcttcagc
      541 cgatttatgg actgactgcg gggctttcta ataagacgat cgtgaagctg atccatcagg
      601 tgcttgatga gcagaagctt cagacagagt atttggcaga tgagtataag gagaggtatc
      661 acctggcaga caggaatttt gcgattcctg cgattcattt tcccaaaaat atgcaggagc
      721 tgttagctgc aaggagaagg ttggtgtttg acgagtttct tctttttatt cttgctgtgc
      781 agtctttgaa ggagaagacg gaggaagcac cgaatgcgtt tcccatgcat ccggtatgga
      841 cgacagagca gattattgaa agtctgccct atgatctgac aaaggcgcag ttgaatgtgt
      901 ggcatgagat tgagagggat ttaagcggcc aggctctgat gtccagactg gttcagggag
      961 atgtaggaag tggtaagacg atccttgcgt ttcttgcaat gatcatgact gtggagaatg
     1021 gatatcaggc agtgcttatg gcacctacgg aggttcttgc aaggcagcat tttcaggcaa
     1081 tggagaagtt gctgcaggaa cagaatattg attttgggca tccggttttg ctgacaggat
     1141 cagataccgc aaaggagaaa agggaaaaat atgtgctgat cgcgtcaaaa gaggctaacc
     1201 tggtgatagg aacgcatgcg ctgattcagg aaaaagtgca gtacaataat cttggactgg
     1261 ttattacaga tgagcagcat cgttttggtg tgaaacagag ggaagcactg actactatgg
     1321 gaaacccgcc taatgtgctg gtgatgagtg ctacaccgat tccaaggacg ctggcgatca
     1381 ttatatacgg ggatctggat atttcagtaa ttgatgaact tcctgcgcag agacttccca
     1441 ttaaaaattg tgtggtggat acttcttata ggccgaaggc ttattccttt atggagaagc
     1501 agatccgtca gggacggcag gtttatgtga tctgtccaat ggtggaggag agcgaaggga
     1561 tggatgggga aaatgtgctg gattataccc tgaagcttcg caatgttttt tcgccggata
     1621 taaagattgc ttctcttcat ggaaagatga aagcgaaaga gaagaatgtg atcatggagg
     1681 cttttgccgc aggggaaatt cagatcctgg tatctactac tgtagttgag gtaggagtga
     1741 atgtgcctaa tgctacagtg atgatggtgg agaatgccga gcgtttcggg ctggcacagc
     1801 ttcatcagct tcgtgggcgt gtcggtagag gggaatatca gtcatattgc atttttatgc
     1861 agggaaatgg tgcaaaggaa atttctaaac gtctgcagat cctgaataaa tccaacgatg
     1921 gattttatat tgcaggagag gatctgaagc ttcgcggtcc tggtgatctt tttggaatcc
     1981 gccagagtgg tctgctggaa tttaaactgg gggacatcta tcaggatgcg gatatcctga
     2041 aagctgccag tgaaacggct tcggagattc tgtcccttga tgcggattta agccttccgc
     2101 agaatgagga acttcagagg agactttcgg catatatgaa ggaagatctg cagaatctag
     2161 gactatagaa aatgacggat acagagtaaa aaataatact gttcatgtta ctgttcataa
     2221 gatgcctaaa ttataaaaac agaaaaacat tgcaaaagca gaagattcgt agtattctat
     2281 tttcataaaa gaatgttact gtttatcata ttttcggtaa cataagaata cctgtaactg
     2341 tacaaaactg agcagttacg aatacttata ccttgtaagg aggctaatca tatggcaaca
     2401 aaagcaacta cagcagatct caacacatcc actgcgaaga caaccgaagt taaagaggct
     2461 cctgcaaaga caactgctga agaagtagct gtaaataaag aagcaaacaa gaaagaaact
     2521 gctgaaacag agactgtaaa gaaagaaaca acagcaaaga aaccggcagc aagaagaaca
     2581 agaaagactg cagaaaagaa atcagcagaa gcagagaaag aaaccgagaa gaaagaaaca
     2641 gctaaagcag cagacactgt agaaaagaaa ccggcagaaa cagagaaaga agccgagaaa
     2701 aaagagacag ctaaaacaac aaagactgct gcaaaaccaa caaaaacaac gaaaagaact
     2761 acaaagaagg ctcctgctaa gacagctgag aaaccggctg aagagacagc tgccgagaag
     2821 aagacaacaa gaagaaccac taagaaagaa gcaacagcag aagtatatat
//
LOCUS       DBMDXG010000290         2848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7766_length_2848_cov_1.617765, whole genome shotgun sequence.
ACCESSION   DBMDXG010000290 DBMDXG010000000
VERSION     DBMDXG010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7766_length_2848_cov_1.617765"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="ACZPD3_08020"
     CDS             <1..256
                     /locus_tag="ACZPD3_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR1415969.1"
                     /translation="KTAKELEEMTGRTYSRIHIVGGGSNAGYLNELTAKATKKEIHAG
                     PGEATAIGNITAQMLKAEEFKTIEEARTIIHESFGVKVYK"
     gene            589..1773
                     /locus_tag="ACZPD3_08025"
     CDS             589..1773
                     /locus_tag="ACZPD3_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR1415970.1"
                     /translation="MNTISVEELEKIDSSKITIVDVRPADQYSRGSFPGAVNIPLDEF
                     EERMESVDREKMVYVLCHTGDRSRDCVEKLSDAGYEAVNIEGGYRAYLRLSLSRFMEN
                     DAKEQKELKTKEIEHSIIKTFRKTVWRPFTKALNEYQLIQEGDKIAVCISGGKDSMLM
                     AKLLQELKRHGKIHFELVFLVMNPGYNADNWKIIQDNAELLGIPLTVFESDIFDTVAE
                     IENNPCYLCARMRRGYLYSHAKELGCNKIALGHHFDDVIETILMGMLYSGKVETMMPK
                     LHSQNFEGMELIRPMYLIKESAIKAWRDTNGLHFIQCACRFTENCVSCGGGRGSKRDE
                     MKELVAQFRNTSSVIETNIFNSVRDINLRTVMGYHKDGEYYNFLDDYDQRGNKGADKE
                     KE"
     gene            1882..2643
                     /gene="larB"
                     /locus_tag="ACZPD3_08030"
     CDS             1882..2643
                     /gene="larB"
                     /locus_tag="ACZPD3_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR1415971.1"
                     /translation="MTLQEILESVKSGSVSVEEAERILKKESYEEMGYAKLDTSRKAR
                     TGFAEVIYCSNKADEHLLNIFERLYREDGEVFGTRASQHQYELVKEKFPETEYDPISR
                     ILKVEKKDKKRIGKIAVCSAGTADIPVAEEAAQTAEYFGTNVERIYDVGVSGMHRLFS
                     RLETIQSANCVIAVAGMEGALASVMGGLVSRPVIAVPTSVGYGASMHGLSALLTMINS
                     CANGIAVVNIDNGYGAGYLATQINRLAAGTDEKGN"
     gene            2646..2753
                     /gene="larC"
                     /locus_tag="ACZPD3_08035"
                     /pseudo
     CDS             2646..2753
                     /gene="larC"
                     /locus_tag="ACZPD3_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
BASE COUNT          844 a          429 c          833 g          742 t
ORIGIN      
        1 aaagacagca aaagagctgg aagagatgac aggcaggact tacagccgta ttcatatcgt
       61 gggcggcggt tccaacgcag gatatctcaa tgagctgact gcaaaggcaa caaagaaaga
      121 aatccatgca ggtccaggag aggcaactgc aatcggaaat atcacagcac agatgctgaa
      181 agcagaagag tttaaaacca ttgaagaggc tcgtactatc attcatgaat cctttggtgt
      241 aaaagtttat aaataataaa aagggaacag ggcgttggct ctgttccttt tgtgtctgcg
      301 ataataagaa aaaaatttca accgggaggt tttaatttta tatatctgag aaggttgtct
      361 gaaaaaaatg aatcgtatat aatgaggtta aaaggtgttt ggaaaatcat tcgttactgt
      421 gaacggagtg aacagtaact gtggctgtga gaaaggagtc aacggcgagg gaatatgttg
      481 actatcatgg ggtttcaaag tataatgatg gctggtgaat tgggcggaaa tacagggtga
      541 ttcatcagag gtaagtgatt gattttaata gataaaggca ggttatatat gaatacaatt
      601 agtgtggaag agctggagaa gattgattct tctaagatta cgatcgtgga tgtgcgaccg
      661 gcggatcagt acagcagggg atctttcccg ggggcagtga atattccgct ggatgagttt
      721 gaggaaagaa tggagtctgt ggacagggag aagatggtgt atgttctctg tcatacgggg
      781 gacagaagcc gggattgtgt ggagaagctg agtgatgcag ggtatgaggc tgtgaatatt
      841 gaaggtggat atcgtgcgta tctgagactt tctctgagcc gttttatgga gaatgatgct
      901 aaggagcaga aggaactgaa gacgaaagag attgagcaca gtattatcaa gacgttcaga
      961 aagacggtct ggagaccgtt taccaaggct ttgaatgagt atcagctgat tcaggaggga
     1021 gataagattg ctgtctgtat ttcaggtggt aaggattcta tgctgatggc gaagctgctt
     1081 caggagctga agcgccatgg gaagattcat tttgaactgg tgtttttggt aatgaatccg
     1141 ggatataatg cggataactg gaagattatt caggataatg cagagcttct gggaattcct
     1201 cttacggttt ttgaaagtga tatttttgat acagtggcag aaattgagaa caatccctgt
     1261 tatctgtgtg cgagaatgag acgtggttat ctgtattctc atgccaagga gctgggatgt
     1321 aataagattg cgttgggaca tcattttgat gatgtgatcg agacgattct catgggtatg
     1381 ctgtacagtg gtaaggtgga gactatgatg ccgaagctgc acagtcagaa ttttgagggg
     1441 atggaattga tccgccctat gtatctgatc aaggaatctg caatcaaggc atggagagat
     1501 accaatggac tgcatttcat tcagtgtgca tgccgtttta ctgagaactg tgtaagctgt
     1561 ggaggcggac gtggttccaa gcgtgatgag atgaaagagc tggttgcgca gtttagaaat
     1621 acaagcagtg tgatcgagac gaatatcttt aacagtgtgc gggatattaa tcttcgtact
     1681 gttatgggat atcataaaga tggagaatac tataatttcc tggatgacta tgatcagcgt
     1741 ggaaataagg gcgctgataa ggaaaaagaa tgataacagc aaaatcttca tttgtggaag
     1801 agaaatacac tgctcagtat tatagtggga acggttgtct gtatgtttct ggtgcaggtg
     1861 gtgttctaaa aggaggaatc catgacctta caggagattc ttgaaagtgt aaaaagtggt
     1921 tctgtatctg tggaggaggc agagagaatc cttaagaaag aaagttatga agaaatggga
     1981 tatgcgaagc tagatacaag ccgcaaggca agaaccggtt tcgcagaagt aatctactgc
     2041 agtaataagg cagatgaaca tcttctgaat atttttgaaa gattatacag agaagacggt
     2101 gaggtattcg gaaccagagc ttcccaacat cagtatgaac tggtgaagga gaagtttccg
     2161 gaaacagaat atgatccgat ctcgcgtatc ttaaaggttg aaaagaaaga taagaagcgt
     2221 attggtaaga ttgcggtgtg ttcagcagga actgcagata ttccggttgc tgaggaagct
     2281 gcacagacag cggaatattt tggcacaaat gttgaacgga tttatgatgt gggagtcagc
     2341 ggaatgcatc gtttattctc cagactggag acgatccaga gtgcgaactg tgtaattgca
     2401 gttgcgggaa tggagggagc tcttgccagt gtgatgggcg gtctggtgag cagaccggtg
     2461 atcgcagtac cgacttctgt gggttatggt gcaagtatgc atggtctttc ggcacttctt
     2521 accatgatca attcctgtgc gaacgggatt gctgtggtga atatcgataa cggctacggt
     2581 gccggctatc tggcaacaca gatcaaccgt ctggcagcag gtacagatga gaaaggaaat
     2641 tgattatggg aaaaacatta tatctggaat gttattcagg gataagtggt gatatgacgg
     2701 ttgcggctct tcttgatctg gggggtgaca gaactgtgtt ggacaaggtg ctgttccggg
     2761 agcgggacgg cgaggaataa gcgaaaatct gcgaaggacc tctggcgcaa tgccgggggt
     2821 ccttttgccg gtatatcccg ggacgagc
//
LOCUS       DBMDXG010000291         2848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7767_length_2848_cov_1.311249, whole genome shotgun sequence.
ACCESSION   DBMDXG010000291 DBMDXG010000000
VERSION     DBMDXG010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7767_length_2848_cov_1.311249"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2838)
                     /locus_tag="ACZPD3_08040"
     CDS             complement(<1..2838)
                     /locus_tag="ACZPD3_08040"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HIR1415972.1"
                     /translation="MKEDMVLLRKEAAKSYTYLYQTEGLVTELREKEVLFFDEGQNEI
                     FRVQAPYMRDFSGSKSESIEVSAEMTADGKCRVTFTPDRNWLNEASRKFPVVIDPVTT
                     TSKAATDIEDAYISSKNNTDNYYNNENLWLKGGNEIRRSFLKFQLPEIKTGDMIVNAR
                     LVMVSLGENGAEKTIAVHKVIQSWESKTINWDNKPIYEETVQDLCKFTADKIKYVVMD
                     ITRMVKEWYRDGSNNGLMLKEIDELSGSVQLMSSDWDSSLSDYRPKIEISYVNYSGLE
                     DYWTYHSQNIGRAGTVHVNDYNGNLILEHRVMETSGSRMPAEVSLVYNTNDKDTNIGY
                     GKGFRLNFHQIIHKKSIAGNVYYAHTDADGTVHYFVEKEVEKDGNTVKEWKDETGLDL
                     TLIRNLKSEEPYTIQNKDGNSMVFNESGYLIAVKDKNGNKLTVSYVNNRVKNITDGAG
                     RIITLNYSLGSDGEEANLIQAVSPSGNKKTFAYTAGRLTTVTDIDGKKVFYTYDSNGM
                     LASAENINGYQVKYGYYTEEPHRVKSIAEYGDGTKGKSLAMTYGYNSTKFTDNKGRKE
                     IYRFDNNGNLLHVHDGFGHAVSCKYNVDGNHVNRLENATKLQDNVVQLLKDPVVQAEN
                     TPWTSKISPKGAGNTEINTIAAECMMGNRSLKAECTSVSGYAYWAQNVKVKKGITYTA
                     SMYVKASVSETAEDGGAILRVRYMDKDGVQQLKDSEIIKKTTEGFVRLTNTFTVPEDS
                     SDTVVKVYMVMWHAKGVMYGDMAQLETGTSANRCNLIDNGDFHLGTTEGFEKNESNTD
                     GLSEVGIEDNIPIKSQLLVTASSQGVLRKTPSDTGEVLSSLTKHQLLSGYITVEKNGR
                     NWYYAKTADGKEGYVSTGQAIPYLGGHEGTNSAFVVVGNSILYKSADKTSARVQEGID
                     AGVCAALVKTVDGTDGNKWYYMGLQIDGNRFF"
BASE COUNT          734 a          677 c          424 g         1013 t
ORIGIN      
        1 gaaaaagcga ttgccatcaa tctgcagtcc catataatac catttatttc catccgttcc
       61 atctacagtt tttacaagag cagcacaaac tcctgcatca attccttcct gtactctggc
      121 actggtttta tctgcacttt tatataaaat agagtttcct actactacga atgctgaatt
      181 tgttccttca tgtcctccca gatatggaat cgcctgacct gttgagacat atccctcttt
      241 gccatctgct gtctttgcat agtaccagtt tcttccattc ttctcgacag taatgtatcc
      301 ggaaagtaac tgatgtttcg tcagactgga cagaacttct cccgtatctg atggggtttt
      361 ccttaaaact ccctgcgaag atgcagttac cagaagctga cttttaatcg gaatattatc
      421 ttcgattcca acttctgaaa gaccatccgt gttgctttca tttttctcaa atccttctgt
      481 agttccaagg tggaaatccc cattatcaat caggttacat ctgttagcgg acgttcctgt
      541 ttcaagctgt gccatatcac catacattac acctttggca tgccacataa ccatataaac
      601 cttaacaaca gtatcactgc tgtcttccgg aacagtaaat gtattagtca ggcgtacaaa
      661 accttctgtt gtctttttga taatttcact gtctttcagc tgctgtaccc catctttgtc
      721 catatagcgg actctcagaa ttgcacctcc atcttctgca gtctcactga cagatgcttt
      781 tacatacatg gatgcggtat aggttattcc ctttttcact ttaacatttt gtgcccagta
      841 tgcatatcca ctcacggaag tgcattctgc cttcagtgaa cgatttccca tcatgcattc
      901 agctgctatt gtattgattt ctgtatttcc ggcacctttc ggagaaatct ttgatgtcca
      961 aggagtattt tcagcctgca ctactggatc tttcagcagc tgcactacat tgtcctgtaa
     1021 cttggtagca ttctcaagac ggtttacatg atttccgtca acattatatt tacagcttac
     1081 agcatgccca aaaccatcat gtacatgaag cagatttcca ttattatcaa atcggtaaat
     1141 ctcttttcgc cctttgttat ctgtaaattt tgtactgtta tatccatacg tcatagcaag
     1201 gcttttacct tttgttccat ctccatactc tgcgatagat tttacacgat ggggttcctc
     1261 tgtataatac ccgtatttta cctgatatcc attaatattc tctgcactgg caagcattcc
     1321 attactgtca tatgtgtaaa atactttttt gccatctata tctgttaccg tggtaagcct
     1381 tcctgcggta taggcaaaag tcttcttatt gcccgatggt gatactgcct gaatcaggtt
     1441 tgcttcttct ccatcgcttc ccagagaata atttagtgta ataattcgcc cggctccatc
     1501 tgtaatattt tttacacgat tattcacata tgaaaccgtt aatttatttc cgtttttatc
     1561 ttttaccgca atcaaatatc cagactcatt aaacaccata ctatttccgt ctttattctg
     1621 tattgtgtaa ggttcttctg attttaagtt tcggatcagg gtcagatcca gccctgtttc
     1681 atctttccac tctttaaccg tattgccatc cttttctact tctttttcca caaaataatg
     1741 aactgtacca tctgcatctg tatgagcata ataaacattc cccgctattg attttttatg
     1801 gataatctga tggaaattta aacgaaatcc ttttccatat ccaatattgg tatccttatc
     1861 atttgtgtta taaaccaacg atacctctgc cggcattctg cttcctgatg tttccatgac
     1921 acgatgttcc agaatcagat ttccgttata atcgtttaca tggacagtcc ctgcacgtcc
     1981 gatattctga ctatgatacg tccagtaatc ttcaagtccg gaatagttta cataagaaat
     2041 ttcaattttg ggtctgtaat cactgagtga agaatcccag tctgatgaca tcagctgtac
     2101 ggaacctgat aattcgtcaa tttctttcag catcagccca ttattactgc catctctgta
     2161 ccactccttt accattctgg taatatccat cacaacatat ttgattttat cagcagtaaa
     2221 cttgcataaa tcctgaactg tttcttcata aattggttta ttatcccagt taatggtttt
     2281 tgattcccag ctctgaatca ctttatgtac agcaattgtt ttctctgcac cattctctcc
     2341 cagcgatacc attacaagac gtgcatttac aatcatgtct cctgtcttaa tctcaggcaa
     2401 ctggaatttc agaaaacttc ttctgatttc gtttcctcct tttaaccaga gattttcatt
     2461 gttataataa ttgtctgtat tatttttaga tgaaatataa gcatcttcaa tatctgtcgc
     2521 tgctttggaa gtagttgtca caggatcaat cactaccgga aacttcctgc tcgcttcatt
     2581 taaccagttt ctatcaggag taaaagtaac tctgcactta ccatcagcag tcatctcagc
     2641 tgacacctca atactttctg attttgatcc tgaaaaatct ctcatatatg gagcctgaac
     2701 cctaaaaatt tcgttttgac cttcatcaaa gaagagtact tctttctccc tcaactcagt
     2761 tacaagtcct tctgtctgat aaagatatgt atatgatttt gcagcttcct ttctaagcaa
     2821 taccatatct tctttcatac gcattccc
//
LOCUS       DBMDXG010000292         2838 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7790_length_2838_cov_2.642674, whole genome shotgun sequence.
ACCESSION   DBMDXG010000292 DBMDXG010000000
VERSION     DBMDXG010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7790_length_2838_cov_2.642674"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="ACZPD3_08045"
     CDS             <1..279
                     /locus_tag="ACZPD3_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR1415973.1"
                     /translation="GNMEVVFDTVNKRGMTMMKKKYMKQVGHADAQMFFYVDSAEELA
                     AKIGGNVKVIAEEPYYRYIPKNGLKLSTKVSMTVSDQFKMVKMIHLKL"
     regulatory      752..866
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1143..2270
                     /gene="ribD"
                     /locus_tag="ACZPD3_08050"
     CDS             1143..2270
                     /gene="ribD"
                     /locus_tag="ACZPD3_08050"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR1415974.1"
                     /translation="MTDQEYMLRAIQLAKKGEGWTNPNPMVGAVIVKDGKIIGEGYHK
                     KYGELHAERNAIASLTESAEGAVIYVTLEPCCHHGKTPPCTEAIIEQKIRKVVIGSRD
                     PNPKVAGKGVQMLREAGVTVVEDFMREECDQLNPVFFHYITTKTPYVVMKYAMTLDGK
                     IATKTGASKWITGESARKEVQHMRHQYMGIMAGIGTVLADDPMLNVRVEGWKSPVRIV
                     CDSKLRIPPGSQIVKSAEKYRTIVAYAEQKNAKEKIKILHTMGVETIYCPDEKNQIDL
                     KKLMADLGNRGIDSILLEGGGTLNDSALRAGIVKEVQAFVAPKLFGGVAGKTPVEGIG
                     VELPSEAVELKYTDICQIGEDIRIKCQVCDKKQEESCLQES"
     gene            2252..>2838
                     /gene="ribE"
                     /locus_tag="ACZPD3_08055"
     CDS             2252..>2838
                     /gene="ribE"
                     /locus_tag="ACZPD3_08055"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR1415975.1"
                     /translation="MFTGIVEEKGKVRYIQLTGESGILAVKARKVLEGTRIGDSIAVN
                     GVCLTVTSIQPDGFTADVMAETIRRSSLGSCKAGSQVNLERAMAADGRFGGHIVSGHI
                     DGTGVIRSMLREENAIWVSIETSPQILHLIVEKGSVCIDGISLTVARVDEAGFQVSVI
                     PHTGEETTLLEKVPGDLVNLENDVIGKYVEKLLGI"
BASE COUNT          931 a          482 c          739 g          686 t
ORIGIN      
        1 ggaaatatgg aagttgtttt tgatacggtg aacaaaagag gaatgaccat gatgaagaaa
       61 aaatatatga aacaggtcgg ccatgccgat gcacagatgt tcttttatgt ggattcagcg
      121 gaagaattgg cagcaaagat cgggggtaat gtgaaggtga ttgcggagga accatactat
      181 cgttacatcc ctaaaaatgg cttaaagcta tccacaaagg tcagtatgac agtttctgat
      241 caattcaaga tggtaaagat gatacatcta aaactatagt agatatacaa tatcagagag
      301 agagaacttt atcatttttt cggacgcact gatgaaacac catcaaaagc cgccttctat
      361 caccagcgtg caaaattaaa agataatgca ctatgggaac ttttgcaaat ctttaaccag
      421 aagcttcaac caactctata cagaaaccgt tatcgtttgg ttgcttgtga cggttctgtt
      481 gctgatgaag tcgatccgga acttctggca aaagtattgg ctaatccaga gatgtgggca
      541 ttgctcaatt cattggcgaa aacgatgaag taatgaaaaa tagtgaaaaa atagtgtaat
      601 tttaatgaaa aaacaatcca taaaacgata taatatatgg ataaaatgag gatatttatt
      661 gtgtatatgg atattctatc tccatcagta aactaagtaa aataaaaatt ctgctttaca
      721 aacaacagat aaagtattaa aataaacaaa aagatatctt cagggcaggg tgagattccc
      781 taccggcggt aaagcccgcg agccgagagg catgatccgg tgagattccg gggccgacag
      841 tacagtctgg atggaagaag atgatagtac agttgatttt ttgtatgaat gaatgtagta
      901 tcagagctct gagatggtga tccatttcag agctttttat ataggaaaat cagacattca
      961 tttatcaatc atatgtaaca gatcaattcc accctgcaga tatttctgca ggcttttttt
     1021 gtatctggta catcgttttc gaaataacta taccattcac ttgtctgtga atccgattgc
     1081 acatgtttaa aagcctcggc gtagcccagg caaagaacag gaataagaaa gaaggaatag
     1141 aaatgacgga tcaggaatat atgcttcggg ctattcaact ggccaaaaaa ggagaaggct
     1201 ggacgaatcc caatcctatg gtaggggcag tgatcgtaaa agacggaaag atcatcgggg
     1261 aaggctatca taaaaagtat ggagaacttc acgcggaacg taatgcaatt gcttctctta
     1321 cagaatcagc agaaggtgcg gtgatttatg tcacgttgga gccttgctgt catcatggta
     1381 aaacacctcc atgtacggaa gcgatcattg aacagaagat cagaaaagtg gtgatcggtt
     1441 ccagagatcc gaatccgaaa gtggcaggaa aaggtgtaca gatgttaagg gaagccggtg
     1501 taacagtagt tgaagatttt atgagagaag aatgtgatca gctgaacccg gtgtttttcc
     1561 attatattac tacgaagact ccttatgtgg taatgaagta tgccatgaca ctggatggga
     1621 agatcgcaac gaaaacagga gcatcaaaat ggatcaccgg agaatctgca cgaaaagaag
     1681 tacagcatat gcggcatcag tacatgggaa tcatggctgg tattggaact gtgcttgcag
     1741 atgatccgat gttaaacgtc cgggtagaag gctggaaaag tcctgtcagg attgtgtgtg
     1801 acagtaagct caggatcccg ccgggcagtc agattgtaaa aagtgcggaa aagtaccgga
     1861 cgatcgtggc ttatgcagaa cagaaaaatg caaaagagaa aataaaaata ttacatacca
     1921 tgggagttga aaccatttac tgtcccgacg aaaagaatca gatagacctt aagaagctga
     1981 tggcagatct tggaaacaga ggcattgaca gcatcctcct ggagggagga ggtacgttaa
     2041 atgacagtgc actgcgagct ggaattgtaa aggaagtaca ggcttttgtg gcaccaaagc
     2101 tgtttggcgg tgtggctggg aagacaccgg tagagggtat tggagttgaa ctgccgtctg
     2161 aagcagtgga actgaaatat acagatatct gccagatcgg tgaggatatc cggataaaat
     2221 gtcaggtgtg tgataaaaaa caggaggaat catgtttaca ggaatcgtag aagagaaagg
     2281 aaaagtccgt tatatacagc tgacagggga atcagggatc ctggcagtga aagcccggaa
     2341 agtcctggaa gggacaagga tcggtgacag tatcgctgtg aatggtgtgt gtctgacagt
     2401 tacgtcgatt cagccggacg gatttactgc cgatgtgatg gcagagacga tcagaagaag
     2461 cagtcttggc agctgcaagg ccggaagcca ggtaaacctg gaacgtgcaa tggctgcaga
     2521 cggcaggttc gggggacaca tagtaagcgg ccatattgat gggactggcg tgatacgttc
     2581 catgctccgg gaagaaaatg ccatctgggt atcaattgag acatcgccac agattttgca
     2641 tctgattgtg gaaaaaggat ccgtctgtat tgatgggatc agcctgacag ttgcaagggt
     2701 tgatgaagct ggttttcagg tgtcggtcat tccacataca ggagaagaaa ccactctttt
     2761 ggaaaaggtg ccgggggatc tggttaatct ggaaaatgat gtgatcggaa aatacgtaga
     2821 aaaactgctt ggcatcag
//
LOCUS       DBMDXG010000293         2800 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7891_length_2800_cov_1.621712, whole genome shotgun sequence.
ACCESSION   DBMDXG010000293 DBMDXG010000000
VERSION     DBMDXG010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7891_length_2800_cov_1.621712"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="ACZPD3_08060"
     CDS             complement(<1..246)
                     /locus_tag="ACZPD3_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR1415976.1"
                     /translation="MTKEELGTLILNSERQLYSTAKTILINDQDCADAIQETIVKAFS
                     KIGTLRNDKYAKTWLIRILINECYTLLRKSSKLVSLEG"
     gene            complement(727..2475)
                     /locus_tag="ACZPD3_08065"
     CDS             complement(727..2475)
                     /locus_tag="ACZPD3_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR1415977.1"
                     /translation="MEVLVLHILKLNIIAAIVILLVKVLATLFKGRVSARWKYFIWLL
                     ITISLCVPVRLPANLALVDFKVLRSSQQNTQNPKITDYAVRPEESQKITETVSSASND
                     MKNLTEIPEQKRTVRYESDKWLGIVAVIFVAVWLSVAVLKLTGELLAYYFSIRNLERM
                     SLQVSDTVSIQMYRAACQKKHVRRIPELRQNAGLTTPLLAGLLHTKLYLPATGYSAEE
                     RKLIFYHELTHYCHRDLWYKMLLRICASIYWFNPFLLIMLKEADKDIENLCDTAVVRR
                     VNKKEHKLYRQLLLRTVAMENQIPYVTASLNDSEMVFKDRILYMVNIRKLRKGILPGI
                     LVTLLLAGGNLVFNVSAGTDTVSVETEKSGIEKNADPEKNNVPDYAPFSEMVTMQKAA
                     ETQDEGGVTENTDTEDSVDEEEKADAETNDEPAMENSGQVSETTDDGITSDNNGSVSS
                     YENLPAGVPYTSGFTTTSGVASIVAPGGGDEESRVLYDNGDGTYSDYYGSRYSYQGDG
                     NWADANGNSYRTWNDEDYYSGNQLEQHELQGSNGTVTVKETTNGDYYYCDADGVGYTD
                     NGDGTWTDENGNIYTE"
     gene            complement(2482..2766)
                     /locus_tag="ACZPD3_08070"
     CDS             complement(2482..2766)
                     /locus_tag="ACZPD3_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR1415978.1"
                     /translation="MTKKKLASTTILSLLTRLENKNFVEVTKQGKMNLYTPLVSQSDY
                     QAHESQSVLEKLYGNSLKKFVTSLYHGKKISSEEVQDLSEFLKNLEDREE"
BASE COUNT          737 a          666 c          475 g          922 t
ORIGIN      
        1 cccttccaaa gaaacaagtt tactcgactt tctcaaaaga gtataacact cattgatcaa
       61 tatacgaatc aaccaagtct tggcatattt gtcatttctc aaagttccaa tctttgaaaa
      121 tgccttaaca atcgtttcct gaatagcatc cgcacaatct tgatcgttaa ttaaaattgt
      181 tttggcagta gaatacaatt gtctctctga attgagaatc aacgtcccta attcttcttt
      241 ggtcacttta acaccacctt ctttagccgg ccggcaattt tcatttacat agattagacg
      301 tacagttttt caaaatatcc ccaaataaac tttatttttt taataataga ggaaataaat
      361 ctttcccctc tgttgacatt atacacctgt cttacttctt acccatatac aattgtgaag
      421 gctttttcca acgtcggtac attaaaaaac gataactatg cccggacctg gttaatccgt
      481 atcttaatca acgaatgtta cagccttgtg cgtaaatcca gtaaatttat cccattggaa
      541 aatctagctg acagacagga gccggaatca tcaacggtat ccaaacagat tttggctttt
      601 tgcacaacat caaaatcaaa gaaagagctt gcagcatttt gcggcttcaa agatttaaga
      661 aacttcattt tgaataaaca aaggagcagg gcattgggta gcatccccat tgtcctgctt
      721 ctttgtttat tctgtataga tgttcccatt ctcatccgtc caggtgccat caccattgtc
      781 tgtgtatccg accccatctg cgtcacaata gtaataatct ccgtttgttg tttcttttac
      841 agtaacggta ccgttactgc cctggagttc atgctgttcc agttggtttc cggaataata
      901 atcttcgtca ttccaggtac gatatgaatt gccattagca tctgcccagt ttccatctcc
      961 ctgataactg tatcggctcc cataataatc agaataggtc ccatctccgt tatcgtaaag
     1021 aactctggat tcttcatctc ctccacccgg tgctacaatt gatgctacac cggaagtagt
     1081 agtgaatcca ctggtatatg gtacgcctgc tggcagattt tcataagatg aaacagaccc
     1141 gttgttgtca gaggtaattc catcatcggt cgtttcactt acctgtccgc tgttttccat
     1201 ggcaggttca tcatttgttt ccgcatccgc tttttcctct tcgtctacag aatcctctgt
     1261 atctgtattc tctgtcactc ctccttcgtc ctgtgtttca gcagctttct gcatagttac
     1321 catttcagaa aacggtgcat aatccggtac attatttttc tctggatctg catttttttc
     1381 aataccagac ttctccgtct ctactgaaac tgtatctgtc ccggcagaga cattaaatac
     1441 cagattaccg cctgcaagca gcagcgtcac aagaattccc ggaagaattc ctttgcggag
     1501 tttccgaatg ttcaccatat acagaatccg atctttaaac accatttcgc tgtcattaag
     1561 acttgctgtc acatatggaa tctgattttc catggccact gtcctgagaa gcagctgtcg
     1621 atataattta tgttcctttt tattaactct gcgaacaacc gctgtatcac acagattttc
     1681 aatatcctta tccgcttctt tcagcatgat aagaagaaag ggattgaacc agtatatcga
     1741 tgcacagatt cgaaggagca tcttatacca gagatcccga tggcaataat gtgtaagttc
     1801 atggtaaaag ataagttttc tttcttctgc tgaatacccg gttgctggta gatacaattt
     1861 tgtatgtaaa agtcctgcaa gaagcggtgt ggtaagacct gcgttttgcc tgagctccgg
     1921 tattctgcgt acgtgcttct tttgacacgc tgcacggtac atctgaatac tcaccgtatc
     1981 acttacctgt agactcatcc gttccagatt ccttatagaa aaataatatg ccagcaattc
     2041 tcctgtcagt ttaagtaccg ctacgctcag ccagacagca acaaaaatca cagccacaat
     2101 tcccagccat ttatctgatt cgtacctgac agttctcttt tgttccggga tttctgtcag
     2161 atttttcatg tcattgctcg cagatgatac ggtttctgtt atcttctgac tttcctctgg
     2221 tctgactgcg taatccgtta tcttcgggtt ttgcgtattc tgttgactgc ttctaagtac
     2281 tttgaaatcc accagtgcca gattcgccgg aaggcgcacc ggaacacaaa ggcttatcgt
     2341 aatcaacagc cagataaagt acttccatct cgctgataca cgtcctttaa acagagtcgc
     2401 aagcactttt accagtaaaa ttactattgc tgcaataata ttcagtttca gaatgtgcag
     2461 aacaagaacc tccataattt gctattcctc cctgtcctcc aggttcttaa gaaattcact
     2521 gagatcctgg acttcttccg agctgatctt ctttccatga taaagtgaag ttacaaactt
     2581 ttttagtgaa ttcccataaa gtttctcaag aacactctgg ctttcatgtg cctgataatc
     2641 tgactgcgaa accaagggtg tatacagatt catttttccc tgtttcgtca cttccacgaa
     2701 atttttgttc tccaatctgg tcagcaatga caaaattgtt gttgatgcaa gctttttctt
     2761 cgtcatttct cactttttct tccagttttt ttctctcttt
//
LOCUS       DBMDXG010000294         2782 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_7943_length_2782_cov_1.368892, whole genome shotgun sequence.
ACCESSION   DBMDXG010000294 DBMDXG010000000
VERSION     DBMDXG010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7943_length_2782_cov_1.368892"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="ACZPD3_08075"
     CDS             complement(<1..310)
                     /locus_tag="ACZPD3_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HIR1415979.1"
                     /translation="MASAELQIPEHIQETQNITREELEMILHTQNPLVTEELRRRAQQ
                     TAKAIYGNKIYVRGLIEFTNYCKNNCYYCGIRCSNTDADRYRLSLEQILSCCETGWNL
                     G"
     gene            complement(326..571)
                     /locus_tag="ACZPD3_08080"
     CDS             complement(326..571)
                     /locus_tag="ACZPD3_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HIR1415980.1"
                     /translation="MESRIAVISIIVENSDSVECLNRILHEFGIYIIGRMGIPYRQRG
                     ISIISVAIDAPLDIINALTGKIGRLAGVSAKTVYSKA"
     gene            complement(611..1591)
                     /locus_tag="ACZPD3_08085"
     CDS             complement(611..1591)
                     /locus_tag="ACZPD3_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1415981.1"
                     /translation="MKTLKKLLFIIMAVAVLAMPVFGVQAAESDIPVTEQLGVSPTPS
                     PVPIRELVTKGNKIYYYYKGKMVKNKWKRYNGYKYYFGANGNAVRGGQRINNVVYVFD
                     EKGRLFENKQNKIVKSGSNIYHIRTEHGRASIGYFIYKNNLYYADPKGRLYQKKSRQN
                     GQLYFTNSGAARKDYNALLKMRVMQIVSSITNSGMSQSQKLYACWKYVVYGGFYYGGP
                     DPNIYQSGWARSEALRMFRAGYGNCYGFSCIFAALAREIGYTPYMICGRVPGSRDGAA
                     DGFTRHCWVKINGLYYDPEAQYAGWMTGVYGYDYYPISHQIQRVVNFCKF"
     gene            complement(1807..>2463)
                     /locus_tag="ACZPD3_08090"
     CDS             complement(1807..>2463)
                     /locus_tag="ACZPD3_08090"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1415982.1"
                     /translation="VKDMPEFFQQEKFDGNSMMCRKLEMLPIECIVRGYITGSGWESY
                     KKTGKVCGIELPEGLKESDKLPEPIYTPSTKAEIGDHDENISYEQSIAHLEKYFPGRG
                     EELAAKLRDNTIALYKKCAEYALSKGIIIADTKFEFGLDKDGNMVIGDEMLTPDSSRF
                     WPAEGYEPGHGQPSFDKQFARDWLKANPDNDWTLPQDIVDKTIGKYLQGYEMLTGKKL
                     "
     assembly_gap    2464..2473
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2474..2679)
                     /locus_tag="ACZPD3_08095"
     CDS             complement(<2474..2679)
                     /locus_tag="ACZPD3_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR1415983.1"
                     /translation="MQEMKPIKEGKVREIYDNGDSLIMVATDRISCFDVILNNQVTKK
                     GTVLTQMSKFWFDMTEDILPNHMIS"
BASE COUNT          746 a          673 c          452 g          901 t
ORIGIN      
        1 aaccaagatt ccatcctgtt tcacagcagg acaaaatctg ttccaaagaa agccggtatc
       61 tgtctgcatc cgtatttgaa cagcgtatac cacaataata acaattgttc ttgcagtaat
      121 ttgtaaattc gatcagacca cggacataaa ttttatttcc ataaattgct tttgcggtct
      181 gttgtgctct tcttcgtagt tcttccgtta ccagtggatt ctgtgtatgt aaaatcattt
      241 ccagttcttc tctggtaata ttctgtgtct cctgaatgtg ttcaggtatc tgcaactctg
      301 ctgatgccat atattttcat ttcctctaag ctttcgaata gactgttttt gctgaaaccc
      361 ctgccagccg gccaattttt ccggtcagtg cattgattat atccagaggt gcatctattg
      421 ccacactgat aatgcttatc cctctctgcc tataaggaat ccccattctg ccgatgatat
      481 aaatgccaaa ttcatgaaga atcctattca gacattctac agaatctgaa ttctctacta
      541 taatactgat cactgcaatt ctgctttcca tagaaacacc tctcatataa ttaatcaact
      601 tatcggcaca ttagaattta caaaagttaa caactctctg gatctggtga ctgatcgggt
      661 aataatcata accataaacc cctgtcatcc aaccggcata ctgtgcttcc ggatcataat
      721 aaagaccatt gatctttacc cagcaatgac gggtgaagcc atctgccgct ccatctctgc
      781 ttcccggtac ccggccacag atcatatacg gtgtatatcc tatttctctt gcaagagcag
      841 caaaaataca ggaaaatcca taacagttgc catatcctgc acgaaacatc ctcaatgcct
      901 cactgcgcgc ccagccactc tggtaaatgt taggatccgg accaccatag taaaatcctc
      961 cgtaaacaac atatttccaa catgcataaa gcttctggct ctgactcata cctgaatttg
     1021 tgattgacga tacaatctgc atcacacgca ttttcagcag agcattataa tcttttcttg
     1081 cagcaccgga attggtaaaa taaagctggc cgttctgtct ggatttcttc tgatacagcc
     1141 ttccttttgg atccgcataa tacagattat tcttatagat aaaatatcca atgctggcac
     1201 ggccgtgttc tgtccttata tgataaatat tggatccgga tttcacaatt ttattctgtt
     1261 tattctcaaa taaacgtcct ttctcatcaa aaacataaac tacattgttg atacgctgtc
     1321 ctccccgcac agcatttcca tttgcgccaa aataatactt atagccatta tatctcttcc
     1381 atttgttctt aaccattttt cccttataat aatagtaaat cttgtttccc tttgtaacca
     1441 gttctcttat gggaaccgga gatggcgtcg gggatactcc gagctgctct gtaacaggaa
     1501 tatcactttc cgcagcctgt acaccaaata ccggcatagc taaaacagca actgccatta
     1561 tgataaaaag caactttttc agtgttttca taaacctttt tctccttaca cattttcata
     1621 atctcagaaa caaaaatata actacactcc atgactacaa tcctgtctct tccattacct
     1681 tcttcacttt agtgtaacaa aaaatttatc tgatttccac atttttgtgg cattttagca
     1741 aaaaatactg ctgtccgaac ttttatcaat gtttcgaaca gcagtatctg ctatatctct
     1801 gaattattat aatttcttac ctgtaagcat ttcataaccc tgaagatatt ttccgattgt
     1861 cttatctaca atatcctgcg gaagtgtcca gtcattgtcc ggatttgctt tcagccagtc
     1921 acgtgcaaac tgtttgtcaa aggatggctg gccatgtccc ggttcatatc cttcagcagg
     1981 ccagaagcga gagctgtccg gtgtgagcat ctcatcaccg atcaccatgt ttccgtcttt
     2041 atccagacca aattcaaatt tggtatctgc gatgatgatt cccttgctta atgcgtattc
     2101 cgcacatttc ttataaagtg caattgtatt atcacgaagt tttgcagcta attcctctcc
     2161 tctgcccggg aaatatttct caaggtgagc gatgctctgc tcataggaga tattttcgtc
     2221 atgatcgcca atctcagcct ttgtggacgg agtatagatc ggctcaggaa gcttgtcaga
     2281 ttccttcagt ccttccggta attcaatgcc gcagacttta cctgtcttct tgtagctttc
     2341 ccagccgctt cctgtaatgt atccacgaac aatacactcg ataggcagca tttcaagctt
     2401 acggcacatc atgctgtttc cgtcaaattt ctcctgctgg aaaaattcag gcatatcttt
     2461 tacnnnnnnn nnngaaatca tgtggttcgg cagaatgtct tctgtcatgt caaaccagaa
     2521 cttggacatc tgagtcagta cagttccctt cttggttacc tgattgttca ggataacatc
     2581 aaagcagctg atgcggtctg tcgcaaccat gatcaggctg tcaccgttgt cataaatctc
     2641 acgtacttta ccttctttga ttggtttcat ttcctgcatt ttttcgcacc tcaatattcg
     2701 ttatgtaatt tgtaaaactc caaaatcatc ggcctttgcc gaaattactg tattaccgta
     2761 ccactatctc tgtaagattg tc
//
LOCUS       DBMDXG010000295         2737 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8099_length_2737_cov_1.750759, whole genome shotgun sequence.
ACCESSION   DBMDXG010000295 DBMDXG010000000
VERSION     DBMDXG010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_2737_cov_1.750759"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="ACZPD3_08100"
     CDS             complement(<1..302)
                     /locus_tag="ACZPD3_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HIR1415984.1"
                     /translation="MKRRATAILLGIMVSSMFLSACGKNEAKEAANESAQVEEEGVGE
                     VTEEGEKVEAENKNASDADDSSKAGDSAKSDEDNKETSVKEEEDGDSSGKDSDDES"
     gene            complement(328..969)
                     /locus_tag="ACZPD3_08105"
     CDS             complement(328..969)
                     /locus_tag="ACZPD3_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1415985.1"
                     /translation="MAKSCNQKGKILYLQKMLSETTSQNPVTMQEILAKLEEQGIRAE
                     RKSIYDDMETLRDFGMDIHYRRGREGGYYEEKSASDKTEADNTIIHQPDGETGEKSEN
                     VQEQIGKESEAPAKISAPIPSQTGENIKEIRLVCQNSTKKKIQRTFGSEIFCKIKGED
                     NFIVTVEVVVDKAFFGWLTSMGRNVHILKPKKAAVAYRDYLKNIAKDYKGIDK"
     gene            complement(972..1694)
                     /locus_tag="ACZPD3_08110"
     CDS             complement(972..1694)
                     /locus_tag="ACZPD3_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR1415986.1"
                     /translation="MKITIGHLYPDLLNLYGDRGNIQCLMKRCQWRGIEAETIPFELD
                     DKIDFSKLDIVLLGGGSDREQMIVCEKLQKIQPDFKAYVEDNGVVIAICGGYQLLGKY
                     YKTDQGNMKGLDLVDLYTEQGEGRLIQNIVLQSELFDMPIVGFENHGGRTCINNNKPL
                     GKVLYGAGNDGKSGYEGVVYKNVIGTYLHGPLLPKNPQLADWLIQHALERKYGKETEL
                     TPLDDSQEKEANDYIYHRFVRG"
     gene            complement(1694..>2737)
                     /locus_tag="ACZPD3_08115"
     CDS             complement(1694..>2737)
                     /locus_tag="ACZPD3_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HIR1415987.1"
                     /translation="LSAGIGGNLKADYACIEVDEASTRRVFPHFKPDYMVLTNLFRDQ
                     LDRYGEIDITMNILKEVMQSAPKMKVIVNGDDALSAYLAMESGNEFITYGISEKVIDD
                     KDSREIREGRFCKQCGEPLLYHFYHYSQLGDYYCPKCGFARPKPDFDAEDVKVGDQLS
                     FCVEGKHIVANYKGFYNVYNILAAYAGVRTAGFAGEHFGDMLRRFNPENGRMEQFRIK
                     GTGVTLNLAKNPAGFNQNISAVMQDQAPKDIIIAINDNAQDGTDISWLWDVDFDLLGN
                     DSVKSITVSGIRCQDMRLRLKYVDIPSVLEGDVEKAIRDRVEDGVGNLYVLVNYTALF
                     STRNILKRLEGEK"
BASE COUNT          668 a          732 c          430 g          907 t
ORIGIN      
        1 tctgattcat catctgaatc ttttcctgaa gaatctccgt cttcttcctc cttcactgat
       61 gtctctttat tgtcttcgtc tgattttgca ctgtctcctg ccttgctgct gtcatcggca
      121 tcagaagcat ttttattttc tgcctctact ttttcacctt cctcagttac ttcgcctacg
      181 ccctcttctt ctacctgggc gctttcattt gctgcttctt ttgcctcatt cttcccgcag
      241 gcagacagga acatggagga taccattatt ccaagcagaa tagctgttgc tcttcttttc
      301 ataatacgat cctttctgat atcatattta tttatcaatt cccttataat cttttgcaat
      361 gttcttcaga taatcccgat aggcaacggc tgctttcttt ggtttcagaa tatgtacatt
      421 tcttcccata gaagtcaacc atccaaaaaa tgccttatca accacaacct caacagttac
      481 aataaaatta tcctccccct ttattttgca gaaaatctcc gaaccaaagg tacgctggat
      541 ttttttcttt gtagaattct gacatacaag cctgatttct tttatattct ctcctgtttg
      601 ggagggtatc ggagcactga tctttgcagg tgcttcagat tcttttccaa tctgttcctg
      661 cacattttca gatttctctc ctgtttcgcc atctggctga tgtattatcg tattatccgc
      721 ttctgtttta tctgaagcgg atttttcctc ataatatccg ccttcccgtc ctcttctgta
      781 atgtatatcc attccaaaat cacgcagggt ttccatatca tcataaatac tctttcgctc
      841 agctcgaatc ccctgttctt ccagttttgc cagaatctcc tgcatggtga caggattttg
      901 gctggttgtc tcagacagca ttttctgcag atacagtatt tttccttttt gattacatga
      961 tttcgccata attatcctct tacaaatcta tggtatatat agtcatttgc ttctttttcc
     1021 tgactgtcat ctaacggtgt aagttctgtt tcttttccat actttctttc cagcgcatgt
     1081 tgaatgagcc agtctgcaag ctgcggattc ttcggaagta acggtccatg cagataagtc
     1141 cctatcacat tcttatatac tacgccttca tagcctgact taccgtcatt tcctgcaccg
     1201 taaagtactt ttcccagtgg tttgttatta ttaatgcagg ttcttcctcc atgattctca
     1261 aatccaacga tcggcatatc aaacagctca ctctgcagaa cgatattctg gatcagacgg
     1321 ccttcgccct gctctgtata caagtctacc aggtccagtc ctttcatgtt tccctgatct
     1381 gttttgtaat acttcccaag taactgatag ccaccgcaga ttgcaatgac aacgccgtta
     1441 tcttccacat aagccttaaa atccggttgg attttctgta gcttctcaca tacgatcatc
     1501 tgttctctgt ccgaaccgcc acccagaagt acgatatcaa gcttcgagaa atctatttta
     1561 tcatccagct caaacggtat ggtctcagcc tcaatcccac gccactggca tctcttcatc
     1621 agacactgga tattacctct gtcaccgtaa agattcagaa gatccggata taaatgtcct
     1681 attgtaattt tcattatttc tcgccctcca gtctcttcag aatatttctg gtactgaata
     1741 atgctgtata gtttaccagc acatacagat tccccactcc gtcctctact ctgtcgcgga
     1801 ttgctttctc aacatcgccc tcgaggacag acgggatatc cacatatttc agccgcagac
     1861 gcatatcctg acagcggata ccgcttacag taatagattt cactgaatca tttccgagaa
     1921 gatcaaagtc aacatcccag agccaggaaa tatctgttcc atcctgagca ttatcattga
     1981 tcgcaataat aatgtccttc ggtgcctgat cctgcatcac tgcggaaata ttctgattaa
     2041 atcccgccgg attcttggca agatttaagg ttactcctgt tcccttaatg cgaaactgct
     2101 ccatacgtcc gttttccgga ttaaatctgc ggagcatatc cccgaaatgc tctcccgcaa
     2161 atcccgcagt cctcactccc gcataagctg ccagtatatt atacacatta taaaatccct
     2221 tataatttgc tacaatgtgt tttccttcta cacaaaagct aagctggtcg cctaccttta
     2281 catcctccgc atcaaaatcc ggcttcggtc ttgcaaatcc gcattttgga caataataat
     2341 ctcccagctg gctgtaatga taaaagtgat acaaaagcgg ctctccacat tgtttgcaga
     2401 atcttccttc ccgaatctcg cgtgaatcct tatcatcaat caccttctca ctgattccat
     2461 aagtgataaa ttcattgccg ctctccatag caagatatgc cgacagagca tcatcgccat
     2521 tgacaatcac tttcatcttc ggcgctgact gcatcacttc ttttaaaata ttcatggtaa
     2581 tatcaatctc tccataccgg tcaagctggt cacggaagag attggtcagc accatataat
     2641 ccggcttaaa gtgcggaaac acccttcttg tagacgcctc atcaacctca atacaagcat
     2701 aatccgcctt taaatttcct ccaattcctg cgctaag
//
LOCUS       DBMDXG010000296         2732 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8121_length_2732_cov_2.515773, whole genome shotgun sequence.
ACCESSION   DBMDXG010000296 DBMDXG010000000
VERSION     DBMDXG010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8121_length_2732_cov_2.515773"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="ACZPD3_08120"
     CDS             <1..511
                     /locus_tag="ACZPD3_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HIR1415988.1"
                     /translation="YDEDIKGKYDYKLTDTFMGFHCGNTPACKMCDSRAVKYQLIQHR
                     LLEPAGSEPDFTRGTLEGDIAASDITFYRLQCNSEGELVSYVAEGEVLDVPTRSFGGI
                     GIFAIKEMGRFYRHVLIEGNYPHHGAVMFGHYGKAFYEVVKFLGLDVKKIGYNQPAGV
                     RYPTENPWG"
     gene            656..2113
                     /gene="xylB"
                     /locus_tag="ACZPD3_08125"
     CDS             656..2113
                     /gene="xylB"
                     /locus_tag="ACZPD3_08125"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR1415989.1"
                     /translation="MYYIGIDLGTSAVKLLLMEESGKICNIVSREYPLFFPHPGWSEQ
                     KPEDWFAQSMEGMKELTKDIDRAQVAGIGFGGQMHGLVTLDKDDNVIRPAILWNDGRT
                     GEETEYLNTVIGKDKLSQYTANIAFAGFTAPKILWMQKNEPENFKKVVKIMLPKDYLA
                     YRLSGSFCTDVSDASGMLLLDVKNRCWSKEMLEICGITEEQLPKLYESWEVVGTLKPE
                     IAKELGFSENVKVVAGAGDNAAAAVGTGTVGDGQCNISLGTSGTVFISSKNFGVDENN
                     ALHSFCHADGSYHLMGCMLSAASCNKWWAEEILQTKDFAAEQAPIQKLGENHVFFLPY
                     LMGERSPHNNPDARGVFFGMSMDSTRADMTQAVLEGVAFGLRDSLEVARGLGIKIERT
                     KICGGGAKSPLWKKIIANVMNLKVDVLENEEGPSMGGAMLAAVGCGAYPDVETIGKKF
                     AKVVDTVEPDPELVAKYEERYQKFRTLYPAMKPLF"
     gene            complement(2225..>2732)
                     /locus_tag="ACZPD3_08130"
     CDS             complement(2225..>2732)
                     /locus_tag="ACZPD3_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HIR1415990.1"
                     /translation="IDNCILDNQIFERYIYQKEQLLLAVPKNYSINTRLQKYQIPIEE
                     IRNGQFRSSHIPSVPLNKFENEPFIILRSDNDTGKRALTICQENHFSPSVVFRLDQQM
                     TAYNIACLGMGITFIGDLLLSRVPTNSELVFYKLPGQSSKRTVFFYWKKGRYISRAME
                     EFLKLPFS"
BASE COUNT          827 a          494 c          672 g          739 t
ORIGIN      
        1 ctatgatgaa gatattaaag gaaaatacga ttataagctt actgatacat ttatgggatt
       61 ccattgtgga aatactcctg catgcaagat gtgtgattcc agagctgtta aatatcagct
      121 gatccagcac agacttcttg agccggcagg atctgaacca gacttcacaa gaggaactct
      181 tgaaggtgat atcgctgcat ctgacattac attctatcgt ttacagtgca attccgaggg
      241 cgagctggtt tcttatgttg cagaaggtga agtactggat gtacctacaa gatcattcgg
      301 tggtatcggt attttcgcaa tcaaagaaat gggaagattc taccgtcacg tactgatcga
      361 gggcaactat ccgcatcatg gagcagttat gtttggacat tacggaaaag cattctatga
      421 ggtagtgaaa ttccttggac tggatgttaa gaagattgga tataaccagc cggcaggtgt
      481 aagatacccg acagaaaatc catggggtta atgagaaatc agtaaaacgg attattaatg
      541 ttcatgtgaa gtgaatgaat ctgaatacag aataaataaa ctgacactat attaaaggca
      601 tcccggggct ttgctctggg atgtattaga ttagaaatag aaaaggagag cacttatgta
      661 ttatattggt attgaccttg gtacttccgc tgtaaaactt cttcttatgg aagaatcagg
      721 aaaaatctgt aacatcgtat ccagagaata tcctttattc ttcccacatc cgggctggtc
      781 tgagcagaaa ccggaagact ggtttgcaca gagtatggaa ggtatgaaag agctgacaaa
      841 ggatatcgac agagcgcagg tagcaggaat tggttttggc ggccagatgc atggtctggt
      901 aacacttgac aaggatgata atgtgatccg tcctgcaatc ctctggaatg acggacgtac
      961 aggcgaagag acagaatatc tgaatacagt gatcggaaaa gacaaacttt cacagtatac
     1021 agcaaatatt gctttcgcag gcttcacagc accgaagatc ctctggatgc agaaaaatga
     1081 gcctgagaac tttaagaagg tagtaaagat tatgcttcct aaggattatc tggcatatcg
     1141 tctgtccggt tctttttgta cagatgtttc tgatgcttcc ggtatgcttc ttcttgatgt
     1201 gaagaaccgc tgctggtcaa aagaaatgct ggagatctgt ggaatcacag aagagcagct
     1261 tccgaaactg tatgagagtt gggaagtagt aggaactctg aaacctgaga ttgcgaaaga
     1321 acttggattc agtgagaatg taaaagttgt tgcaggtgca ggtgacaatg cggcagctgc
     1381 tgtaggaaca ggtacagttg gtgacggtca gtgtaatatt tctcttggaa catccggaac
     1441 tgtgtttatt tccagtaaaa actttggtgt ggacgaaaat aacgcacttc attctttttg
     1501 tcatgcagat ggaagctatc atctgatggg atgcatgctc agcgcagctt cctgtaacaa
     1561 atggtgggca gaggagattc ttcagacaaa ggatttcgca gcagagcagg ctccaattca
     1621 gaagctggga gagaatcatg tattcttcct tccatatttg atgggagaac gttcacctca
     1681 taataatcct gatgcaagag gtgtattctt cgggatgtcc atggacagca caagagcaga
     1741 catgacacag gctgttctgg aaggtgttgc ctttggtctt cgtgattccc tggaagttgc
     1801 aagaggcctt ggaatcaaga ttgagagaac caagatctgc ggtggcggag ctaagagccc
     1861 actgtggaag aagattattg caaacgttat gaacctgaag gttgatgttc tggagaacga
     1921 agaaggaccg tccatgggtg gagctatgct ggcagcagta ggctgtggtg cttacccgga
     1981 tgtagaaacc atcggtaaga aatttgccaa agttgttgat acagtagaac cggatcctga
     2041 actggttgca aaatatgaag agagatatca gaagttcaga actctgtatc cggcgatgaa
     2101 gccattgttc taatcgaata tctgcctgac atactggggt tgttgtgctt atttagcaca
     2161 acaaccccat tttgacgtac atctgatgat gaccaatcac catagcctat ttgattatga
     2221 catatcagga aaagggcagc ttcaggaatt cttccatggc tctgctgata tagcggcctt
     2281 tcttccagta aaaaaacaca gtgcgtttac tgctctgacc gggcagctta taaaaaacaa
     2341 gttctgaatt tgttggaact ctggagagca gaagatcccc gatgaaagtg atacccattc
     2401 ccaaacatgc aatattatag gcagtcatct gctgatccag gcgaaatact acagaaggcg
     2461 aaaagtgatt ttcctgacaa atggtaagtg cacgcttacc ggtatcatta tctgaacgaa
     2521 gaataataaa tggttcgttc tcgaatttgt ttaatggaac agaaggaata tgagaagaac
     2581 ggaactgccc gttccggatt tcctcaatag gaatctgata tttctgcagt ctggtattga
     2641 ttgaataatt tttgggaact gcaagaagaa gttgttcttt ctggtagata taacgttcaa
     2701 aaatctggtt atccagaata cagttatcaa tc
//
LOCUS       DBMDXG010000297         2732 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8122_length_2732_cov_1.693273, whole genome shotgun sequence.
ACCESSION   DBMDXG010000297 DBMDXG010000000
VERSION     DBMDXG010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8122_length_2732_cov_1.693273"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="ACZPD3_08135"
     CDS             complement(<1..1029)
                     /locus_tag="ACZPD3_08135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR1415991.1"
                     /translation="MKSTERLIFHIDVNSAFLSWESARRVSQGLPDLREIPSCVGGDP
                     KKRTGIVVAKSIPAKKYGIQTGEPVAMALRKYPNLVIVPSDFELYDKCSRAFKAICAS
                     FAPVMESFSIDEVFLDMTGTSLIYPDPVAAAHELKGKIHRELGFTVNVGISTNKLLAK
                     MASDFQKPDRVHTLFPDEIPEKMWPLPIRNLLFLGKASEQKLLNQGIRTIGDLARERA
                     SNVQYWLGEKTGHQLWQYARGIDDSPVKAVPDEAKGFSVETTFNDDITSIEQVFPILL
                     EQCDVLATRMRRKDKKCNCISVTFRTLDFRNRSHQTKLENATDLTDEIYINATRLFKE
                     FWKGQPLRL"
     gene            1792..2505
                     /locus_tag="ACZPD3_08140"
     CDS             1792..2505
                     /locus_tag="ACZPD3_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR1415992.1"
                     /translation="MYLSDIHTHSIASGHGTTCTISDMAKAASKKGLKLLGISDHGPG
                     TLASGTSSYFRSLPFYPRKRFGIDILYGVELNILDSAGHTDLSDELLNNLDYAIISMH
                     RQNYRSGSVSQNTEAYINAMKHPAVKILGHCDDTHFPVDYETLARAALRENVIFEINE
                     ASLAPGGYRGDTRANAARILYLCQKYQLPIILSSDSHGKEHVGDFTYAEEFVHQLMFP
                     ETLILNNQLPKLKVFLQTR"
     gene            2665..2732
                     /locus_tag="ACZPD3_08145"
                     /pseudo
     CDS             2665..2732
                     /locus_tag="ACZPD3_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          751 a          631 c          495 g          855 t
ORIGIN      
        1 cagacgtagc ggctgtcctt tccagaattc tttaaaaagt cttgtagcat ttatataaat
       61 ttcatctgta agatctgtag cattttccag ctttgtctgg tgtgaacggt ttctgaaatc
      121 caaagtacgg aaagtcacgg aaatacagtt gcatttctta tcttttctgc gcattctggt
      181 tgccagaaca tcacactgct ccaaaagtat gggaaacacc tgctctatgg aagtaatatc
      241 atcgttaaaa gtagtttcca cgctgaaacc ttttgcctca tcaggcactg cttttacagg
      301 ggaatcatcg atacctctgg catattgcca tagctgatgt cccgtttttt ccccaagcca
      361 gtactgcaca ttggatgctc tttctcttgc cagatcaccg atggtacgga ttccctgatt
      421 cagaagcttc tgctcggaag cttttcccag aaacagcaga ttacggatag gaagaggcca
      481 cattttctcc gggatttcat ccgggaacag agtgtgtact ctgtccggct tttgaaaatc
      541 tgatgccatc tttgccaaaa gcttattggt agaaattcct acatttactg taaatcccag
      601 ttctctgtga atcttgcctt tcagctcatg agcagcagct acagggtcag gatagatcag
      661 agaagtacct gtcatatcca gaaatacctc atcaatggaa aaagattcca tcacaggtgc
      721 aaaggaagca cagattgctt taaatgcccg ggaacattta tcgtataatt caaaatcact
      781 gggaacgatc accagattgg gatattttcg aagagccatg gctactggct cgccggtctg
      841 gatcccgtat ttttttgcag ggatagattt ggcaaccacg atacctgtcc gtttctttgg
      901 atctcctcct acacaggaag gaatttctct gagatccggc agcccctggc tcacccttct
      961 ggctgattcc caggaaagaa atgcactgtt tacatcaata tgaaaaatta accgttccgt
     1021 acttttcact gttcttcctc caatatatga attgcgctct tcatatctga tgatttcatt
     1081 ttaccctgat ctgctctcca gggtaagaac ctcgtttgtc caaaaaagtg tctttatttg
     1141 catcatcttg tctgagctgt tactgtattt atatttgttt gatttcactc tgctatcaga
     1201 gttttgaaca acaacgccgc acttgccata cggcagtgcg gcgtctgtat ttcgctaccg
     1261 gtcacattgt tcccggtaac tttttttact taattctgag gattcatttc ctctcgatat
     1321 atacttatcg attagtcata aagattagga gcaatttctt cgctgtccat atttgtggaa
     1381 tcataccagt atccagttca gcctccagcc attcataaga gaattccggg aatgtctgat
     1441 gtttaaaatt tttccatgtt actcctttct catgaagtaa ctgtctacac ataagcaaac
     1501 agtgccatcg ctgtcattaa tcagcaataa gcccccgtgt tttcatatag ttccattata
     1561 ccttctcatt ttcccgaagt caacataatt taacatgtat cagccgttta tttgtgttgc
     1621 tttttatttg atttttgttg tatttcagtt gtttttattt tgtttcctca ttgacattta
     1681 aatcctcatc tgtcatactt gaatataacg aatgtaccat caaacagcag ctttgcatct
     1741 tccgtaaggc tcttatcata tatagaaacc gctctggaaa ggagacaaac aatgtatctt
     1801 tctgacatcc acacacattc tatcgccagc ggtcacggaa ccacctgtac catctctgac
     1861 atggcaaaag cagccagcaa aaaaggactg aaactccttg ggatttctga ccacggcccg
     1921 ggaactctgg cttccggaac ttcttcttat ttcagaagcc ttccttttta tcccagaaaa
     1981 agattcggca tagatattct ttacggagtt gaactaaaca tcctagacag tgcaggacat
     2041 acagatctgt cagatgaact gctgaacaac ctggattacg ctatcattag tatgcatcgg
     2101 cagaactacc gttcaggctc tgtatctcag aatacagaag cttacatcaa tgcaatgaaa
     2161 catcctgctg taaaaatact cggccactgc gacgatacac atttccctgt agattatgaa
     2221 acgctggcaa gagcagcttt acgggaaaat gttatttttg aaataaacga agcttccctc
     2281 gcacccggag gatatcgggg agacacaaga gccaatgccg ccagaatcct gtatctctgt
     2341 cagaaatatc agcttcctat tattttgtcc agcgacagcc acggaaaaga acatgttgga
     2401 gatttcacct atgccgaaga attcgttcac cagctcatgt ttccggaaac tcttatcctt
     2461 aacaaccagc ttcccaaatt aaaagttttc cttcagacac gttaaaagaa tgttcctcaa
     2521 agggacattc ttttatttta tgcattgttt ttttcattgt gttattttgt ttattttcta
     2581 taatttctta tcaaaaaaga tgacatttta gtacatttta actaacaaag ctcacttaac
     2641 catttacata tctcgtgata gatcagtctt actgtaggaa ccaccgagga ccccacagta
     2701 tcccgaatga ctatcgcaac agtcagcgat ga
//
LOCUS       DBMDXG010000298         2718 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8179_length_2718_cov_1.330531, whole genome shotgun sequence.
ACCESSION   DBMDXG010000298 DBMDXG010000000
VERSION     DBMDXG010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_2718_cov_1.330531"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            324..1364
                     /gene="hrcA"
                     /locus_tag="ACZPD3_08150"
     CDS             324..1364
                     /gene="hrcA"
                     /locus_tag="ACZPD3_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR1415993.1"
                     /translation="MDQELDDRKKKILKAIIKTYMETGEPVGSRTISKFADLNVSSAT
                     IRNEMSDLTDMGYIVQPHTSAGRIPSDKGYRLYVDELMKEKESEVQEIKDLMIEKTDK
                     LDKMLKQVAKVLASNTNYATMISVPQYSGSKIKFIQLSRVTPLQLVAVVVSDNNVIRN
                     QIINLDEEMDDQTILKLNLLLNTNLNGVPIQDINLGMIARLKEQAGVHGSVVGTVLDA
                     VADTIQVDEDDMQIYTSGATNIFKYPELADTSKASELISAFEEKQDLVDLVKERMSDT
                     ENTGIQVYIGDEMPVQTMKDCSVVTATYELGKGMHGTIGIIGPKRMDYANVVDSLKAL
                     KVQLDELIKKES"
     gene            1382..2026
                     /gene="grpE"
                     /locus_tag="ACZPD3_08155"
     CDS             1382..2026
                     /gene="grpE"
                     /locus_tag="ACZPD3_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR1415994.1"
                     /translation="MSEETKNTAQEEETTDTPVTEETVENEPEVVENGEAETEEIPVE
                     DGDEEASQDDKKDSKSKTSFFGKKKKEKDKFEQQIEELTDRLKRNMAEFDNFRKRTEK
                     EKSSMYIIGAKDIVEKMLPVVDNFERGLAQAPEGDSFADGMKMIYKQLITTLDELGVK
                     PIEAVGKEFDPNFHNAVMHVEDEEAGENIVVEEFQKGYTYKDFVVRHSMVKVAN"
     gene            2087..>2718
                     /locus_tag="ACZPD3_08160"
     CDS             2087..>2718
                     /locus_tag="ACZPD3_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR1415995.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGQPTVIANTEGARTTPSVVAFTK
                     TGERLVGEPAKRQAVTNAERTISSIKREMGTDYRVTIDDKKYSPQEISAMILQKLKKD
                     AEGYLGEKVTEAVITVPAYFNDAQRQATKDAGKIAGLDVKRIINEPTAAALAYGLDNE
                     KEQKIMVYDLGGGTFDVSVIEIGDGVIEVLSTAGNNRLGGDDFDQKVTDY"
BASE COUNT          952 a          531 c          641 g          594 t
ORIGIN      
        1 gttatgagcc ggctgcatca cctgtctgag cggagtctgc gacggagcga gtttgacaag
       61 ccggctcatg cctttagcag atttctgcag acttagtatc gcttagcgtc cgaaaccgca
      121 cacaatatca ataatttctc cccgcgtccc ctttctataa cataatctta ataaccccta
      181 ttgttcataa aataacaaaa aattcacaaa ataatattga caaaccaaat caagagtgct
      241 atcttatgag cataaggtgt tagcactcct aaaacatgag tgctaacaaa ccaaaaacaa
      301 aggcagtaaa ggagtggaac ttcatggatc aggaattaga tgatcgtaaa aagaaaatac
      361 taaaagccat catcaaaacc tacatggaaa caggcgaacc ggtgggttcc cggaccattt
      421 ccaagtttgc agatttaaat gtaagctctg ccaccatccg gaatgagatg tcagacctga
      481 cagatatggg atatatcgta cagccccaca cctcagccgg aagaattcct tctgataaag
      541 gttatcgcct ctacgtagac gagctgatga aagaaaagga atcagaggtt caggaaatca
      601 aggatctcat gatagagaaa actgacaaac tggataaaat gctgaagcag gtagcaaagg
      661 tactggcctc caataccaac tatgcaacaa tgatctctgt tccacagtac agcggaagca
      721 agattaaatt catccagctt tcccgtgtga caccccttca gcttgtggca gtagtagtga
      781 gtgataacaa tgtcatccga aaccagatca tcaatctgga tgaggagatg gacgatcaga
      841 caatcctgaa attaaatctt cttcttaaca ccaacttaaa tggggtgccc attcaggata
      901 tcaacctggg aatgattgcc cgcctgaaag aacaggcagg tgtacatggc tctgtggttg
      961 gcacagtcct tgatgcagtg gcagacacca tccaggtaga cgaagacgat atgcagatat
     1021 atacttcagg agcaaccaac attttcaagt atccggagct tgcagacaca agcaaagcga
     1081 gcgagctgat ttcagcattt gaagagaagc aggatctggt agacctggtg aaagagagaa
     1141 tgtctgatac cgagaacaca ggaattcagg tgtacattgg tgatgagatg ccggtgcaga
     1201 ccatgaaaga ctgcagtgta gtcacagcca cttacgaact tggaaaagga atgcatggta
     1261 ccatcggtat catcggtccc aagcgtatgg attatgcaaa cgtagttgac agcctgaagg
     1321 ctttgaaagt ccagctggac gaactgatta agaaagagag ttagaaaggc ggtaatagag
     1381 agtgtcagaa gaaacaaaga acacagctca ggaagaagag acaacagata ctccggtaac
     1441 agaagaaact gttgagaatg aacctgaagt tgtagagaac ggcgaagcag aaacagagga
     1501 aatcccggta gaggacggag acgaggaagc ttcccaggat gacaagaaag acagcaaatc
     1561 aaaaacttct ttcttcggca agaagaaaaa agaaaaagat aaatttgaac agcagattga
     1621 agaacttaca gacaggctga aacgaaacat ggctgaattc gataacttcc gtaagagaac
     1681 ggagaaagag aaatccagta tgtatatcat cggagccaag gacatagttg agaaaatgct
     1741 tccggttgtg gataacttcg agagaggtct tgcacaggca cctgaaggag attcttttgc
     1801 agatggcatg aagatgatct acaaacagct gatcaccaca ttagatgagc tgggcgtgaa
     1861 accgattgaa gcagtgggta aggaatttga tccgaacttc cacaacgcag taatgcatgt
     1921 ggaggatgaa gaagctggtg agaatattgt ggtagaagaa ttccagaagg gttacaccta
     1981 caaggatttc gtagtccgcc acagtatggt gaaagtagcc aactaattta catgaaaata
     2041 tagaatatat caaatctagt tgaaaataaa caggaggaac aagattatgg gaaaaatcat
     2101 tggtattgac ttaggtacaa caaacagctg tgtagccgta atggaaggcg gacagccaac
     2161 agttattgct aatacagaag gcgcaagaac aacaccatca gttgtcgctt tcacaaaaac
     2221 aggagaacgt cttgtaggtg aacctgcaaa acgtcaggca gtaacaaacg cagagagaac
     2281 aatctcttcc attaaaagag agatgggtac tgactatcgt gtaacaatcg acgacaagaa
     2341 atattctcca caggaaattt ccgctatgat tcttcagaaa ctgaaaaaag atgcagaagg
     2401 ataccttggt gagaaagtta cagaagcagt tatcacagtt cctgcatatt tcaatgatgc
     2461 tcagcgtcag gcaacaaaag atgccggtaa aatcgcaggt cttgatgtaa aacgtatcat
     2521 caacgagcca acagcagcag ctcttgcata tggtcttgac aatgaaaaag aacagaagat
     2581 tatggtatac gatttaggtg gcggtacatt cgatgtatct gtgatcgaaa tcggtgacgg
     2641 cgttatcgaa gtactttcca cagcaggtaa caaccgtctt ggtggtgatg acttcgacca
     2701 gaaagttaca gattacat
//
LOCUS       DBMDXG010000299         2707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8216_length_2707_cov_1.921335, whole genome shotgun sequence.
ACCESSION   DBMDXG010000299 DBMDXG010000000
VERSION     DBMDXG010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8216_length_2707_cov_1.921335"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="ACZPD3_08165"
     CDS             <1..434
                     /locus_tag="ACZPD3_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR1415996.1"
                     /translation="FYMDFNGMEVFHNEDDSYLRSVHSLVDKNAKDYLNSVYINKNVL
                     PERIEIKSRDSAGYVTEILADGKVLEGEAFRKGMGLTSSNFTIQKSGKEIRFLCRGKG
                     HGLGFSQYGGNEMAKESANAKEILQYYFPEMDVLNIKSVNC"
     gene            496..1389
                     /locus_tag="ACZPD3_08170"
     CDS             496..1389
                     /locus_tag="ACZPD3_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR1415997.1"
                     /translation="MMNNKVTRIIANSVGLAAVAALGITVYQLGTSPVKEKTPEEKTE
                     NVGESTQDTEEQLSEDAGTNHVESENQWISDDGEDLDDTKVTFQKQGMYGETAQNTVD
                     NNESDNTADVDKTDNAVNSQQAEESSVNANMKSQTISEDQADEATDVSASALNLATVN
                     FSEDTLMEWPVNGNVLLDYSMDQTTYFPTLDQYKLSPAIAVGAVEGAPVVAAVNGKVY
                     SIEQNAQTGTTLTMELGNGYQAVYGQLTDLTVSEGDTIKKGTTIGYIAQPTKYYSTEG
                     TNLYFAMKKNGEPIDPIEYLP"
     gene            1547..1651
                     /locus_tag="ACZPD3_08175"
     CDS             1547..1651
                     /locus_tag="ACZPD3_08175"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1415998.1"
                     /translation="MDIRLGAKSPTNCDIHLAESIFQTRSSIIKYCWL"
     gene            1700..2692
                     /locus_tag="ACZPD3_08180"
     CDS             1700..2692
                     /locus_tag="ACZPD3_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR1415999.1"
                     /translation="MNINEIAKLAGVSRATVSRYLNNGYVSEEKKKNISRVIEETGYQ
                     PSSQAQRLRTKKTKLVGVILPKIDSNTISREVAGISDILTKKGYQLILANTNNSVEEE
                     LKYLSLFRDNQVDGVIFIATILTKQHREMMKGFKVPIVLLGQQLDGYPCIFQDDYKAA
                     VNLTEQMVKTGKKFGYITVTDKDEAVGRQRKSGVEKVLGENQITLESGCVKCGNFTLE
                     SGYEKAKELFTEHPDVDTLICATDTMAVGAANWLKENGYQIPQQVQIAGMGDSFLGKI
                     IEPKLTTVHFFYKTSGMESAKVLVDLIESKETSSVHKEIKMGCKVVLRESHRNI"
BASE COUNT          969 a          415 c          647 g          676 t
ORIGIN      
        1 agttctacat ggacttcaat ggaatggaag ttttccacaa cgaagatgat tcgtatctca
       61 gatccgtaca cagtcttgtg gataaaaatg caaaagatta tttgaatagt gtatacataa
      121 ataaaaatgt ccttccggaa agaattgaaa taaaatccag ggacagtgcc ggttatgtta
      181 cagaaattct ggctgacggg aaagtgttgg aaggcgaggc attcagaaaa ggaatgggac
      241 tcacgtcctc aaattttaca attcagaagt ctggaaagga aatacgattt ttatgtagag
      301 gaaaaggtca tggattagga ttctcacaat atggagggaa tgagatggca aaggagtctg
      361 cgaatgcaaa agagatctta caatattatt ttcctgagat ggatgtgctg aacataaaat
      421 ctgtaaattg ctgaatagat acgaatattc tggacaccct atagatatga agatgaaaac
      481 agaggtgaaa ggaatatgat gaataacaaa gtgacaagaa tcattgccaa ttccgttgga
      541 ctggccgctg tagcagcatt gggaattaca gtttatcagt taggtacctc cccggtcaaa
      601 gagaaaactc ctgaagaaaa aacagaaaat gtcggtgaaa gtacacagga tacggaagaa
      661 caattgagtg aagatgcagg aacgaatcat gtggagtcag aaaatcagtg gatatccgat
      721 gacggggaag atttggatga tacaaaggtt acattccaaa aacaaggaat gtatggggaa
      781 acagctcaga atactgtgga taataatgaa tcagataata ctgcagatgt ggataagact
      841 gacaatgctg taaattctca gcaagcagaa gaatcatcag taaatgcaaa tatgaaatcc
      901 cagacgatat cagaagatca ggcagatgaa gcaacagatg tttcggcatc ggccctgaat
      961 cttgcgacag taaatttttc ggaagataca ttaatggagt ggccggtgaa tggaaatgtt
     1021 ctccttgatt acagtatgga tcagactact tattttccga cgcttgatca gtataaattg
     1081 agtccggcta ttgctgtcgg tgcagtagag ggagcacctg tggtagctgc tgttaatggc
     1141 aaggtttatt ctatagagca gaatgcgcag acgggtacta cactgacaat ggaacttgga
     1201 aatggatatc aggctgttta tggacagctt acagatctga cagtgtcaga aggagataca
     1261 attaaaaagg gaacgacgat aggatatatt gcacagccta caaaatatta cagcacagaa
     1321 ggaaccaatc tttattttgc aatgaaaaag aatggagaac caatagatcc gattgaatat
     1381 cttccataat gaaatagaag aaaaaaacag ggttgccgca gattggcagc ccttaatttg
     1441 gtatagggcc aagacagtaa cgtataaaca gtactttgat atttgcaaca gaaatgatat
     1501 gtgtagtata atacaaaata tgtagtgatt cagcagttaa caattcgtgg atatcagatt
     1561 aggggcaaaa tctcccacaa attgtgacat acatcttgcg gaaagcattt ttcagacacg
     1621 ctctagcatc ataaaatact gctggttata aaaatacttt cggaaattat acagacagca
     1681 tgaaagagga gctttttata tgaatattaa tgagattgcc aaactggcag gggtctccag
     1741 agctaccgta tccaggtatc tgaacaatgg ttatgtcagt gaggaaaaga aaaaaaatat
     1801 cagcagagtt attgaagaaa ccgggtatca gccttcttca caggcgcaga ggctgcgtac
     1861 taagaagaca aaactggtcg gagtaattct gccaaagata gattccaata cgatcagccg
     1921 ggaagttgcc ggaatcagtg acatacttac caaaaaagga tatcagctga tactggcaaa
     1981 tacaaataac agtgttgaag aagaattgaa atatctttcg ctgttcaggg ataatcaggt
     2041 cgatggtgtt atttttattg ccaccatatt gacaaaacag cacagggaaa tgatgaaggg
     2101 atttaaagtt ccgattgttt tacttggaca acagctggat ggttatccat gtatttttca
     2161 ggacgattat aaggctgctg taaatcttac tgaacagatg gtgaagacag gaaagaagtt
     2221 tggatatatt acagttacgg ataaagatga ggcagttggc aggcagcgaa aatcaggtgt
     2281 ggagaaggtt cttggagaaa atcagatcac attggaaagt ggatgtgtaa aatgcggaaa
     2341 tttcacatta gagtcaggat atgagaaagc gaaagaactt tttacggagc atcccgatgt
     2401 agatacattg atctgtgcaa cagataccat ggcagttgga gcggcaaatt ggctgaagga
     2461 gaacggatat cagattcctc aacaggttca gattgccgga atgggagaca gttttctggg
     2521 aaagatcata gaaccaaagc tgactactgt acattttttt tacaagacaa gtggaatgga
     2581 atcagcaaaa gtgctggtag atttgataga atctaaggaa acatcatctg tacataaaga
     2641 aataaaaatg ggatgtaagg tggtactcag agagtctcac cgaaatatat gactgaacat
     2701 gaaagag
//
LOCUS       DBMDXG010000300         2705 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8226_length_2705_cov_1.444700, whole genome shotgun sequence.
ACCESSION   DBMDXG010000300 DBMDXG010000000
VERSION     DBMDXG010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8226_length_2705_cov_1.444700"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            41..1231
                     /locus_tag="ACZPD3_08185"
     CDS             41..1231
                     /locus_tag="ACZPD3_08185"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR1416000.1"
                     /translation="MNVLVINCGSSSLKYQLINSDSEAVLAKGLCERIGIDGRLVYQK
                     TGCDKEITEAAMPTHKEAIQMVLDALTNDKTGAIGSLKEVNAVGHRVVHGGEKFAKSV
                     VITDEVISAVEECNDLAPLHNPANLIGIRVCSELMPGVPQVAVFDTAFHQTMPAKAYL
                     YGLPIEYYKNYKVRRYGFHGTSHSFVSKRAVEFLGLDKDNSKVIVCHLGNGSSISAVV
                     NGECVDTTMGLTPLEGVVMGTRSGNIDPAIMEFIAKKENLDIAGMMNVLNKKSGLLGL
                     SGGLSSDFRDLNDAAQSGNEDAANAIDVLCYGIAKFVGGYVAAMNGVDAIVFTAGIGE
                     NAIPVREKVVSYLGYLGVTLDKEANGVRGEEIVISTPDSKVKVAVIPTNEELAICRET
                     VALV"
     gene            1506..2033
                     /locus_tag="ACZPD3_08190"
     CDS             1506..2033
                     /locus_tag="ACZPD3_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR1416001.1"
                     /translation="MQIHLSDISSSEGMRIQKTAEFGMDTITFQSGSFPVLAKEPIEL
                     TITNTGDRNLEIRGTGKITVGIPCDRCLEEVSTEIPLEIERKLDMKLTDEDRVNDLDE
                     SSYLTGMDLDVDQLVYLEVLMSWPLKVLCREDCKGICSQCGKNLNDGPCGCVEEPKDP
                     RMAAISDIFSKFKEV"
     gene            2038..2220
                     /gene="rpmF"
                     /locus_tag="ACZPD3_08195"
     CDS             2038..2220
                     /gene="rpmF"
                     /locus_tag="ACZPD3_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR1416002.1"
                     /translation="MSICPKNKSSKARRDKRRANWKMSAPNLVKCSKCGELMMPHRVC
                     KACGSYNKKEIIKVED"
     gene            2306..>2705
                     /locus_tag="ACZPD3_08200"
     CDS             2306..>2705
                     /locus_tag="ACZPD3_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1416003.1"
                     /translation="MNEKEKVNQITEGVIWKQLLLFFFPIVFGTFFQQIYNTADTIVV
                     GRFVGKQALAAVGGSASQIANLIVGFFVGLSSGAAVVISQFYGAKDKKNLSKALHTAF
                     AFSIVAGIVLTVVGIFLTRPALMLMKTPADV"
BASE COUNT          829 a          485 c          650 g          741 t
ORIGIN      
        1 atggacagta ttcgaaattt gctgccggca gaatatgaaa atgaacgtat tagtaatcaa
       61 ctgtggaagt tcatccctta aatatcagct gatcaactct gattcagaag cagttcttgc
      121 aaaaggtctc tgcgagagaa tcggtatcga tggaagatta gtatatcaga aaacaggctg
      181 tgacaaagag atcacagaag ctgcaatgcc tacacataaa gaagcaatcc agatggtact
      241 ggatgccctc acaaatgata aaacaggtgc aatcggaagc ctgaaagaag taaatgctgt
      301 tggacatcgt gttgtacatg gtggtgagaa atttgccaaa tctgtagtga tcacagatga
      361 agtaatctca gcagtagaag aatgcaatga ccttgcacca cttcataacc ctgcaaacct
      421 gatcggtatc cgtgtatgct ccgagcttat gccgggtgta cctcaggtag cagtattcga
      481 tacagctttc catcagacaa tgcctgcaaa agcatatctc tatggtcttc caatcgaata
      541 ttacaagaac tacaaagtaa gaagatatgg tttccacgga acatcccaca gctttgtttc
      601 caaacgtgct gttgaattcc ttggccttga caaagataac tccaaagtta tcgtctgcca
      661 ccttggaaac ggttcttcca tcagtgctgt tgtaaacggt gagtgtgttg atacaacaat
      721 gggtcttact ccacttgaag gtgttgttat gggaacacgt tccggtaaca ttgatcctgc
      781 tatcatggaa ttcattgcta agaaagagaa ccttgacatc gcaggtatga tgaatgttct
      841 caataagaaa tccggtcttc ttggattatc cggcggatta tccagtgact tccgtgacct
      901 gaacgatgca gctcagagcg gaaacgaaga tgcagctaac gcaatcgatg ttctctgcta
      961 tggtatcgct aaattcgttg gcggatatgt tgcagctatg aacggtgtag atgccatcgt
     1021 atttacagca ggtatcggcg agaatgctat tcctgtacgt gagaaggttg taagctatct
     1081 tggatacctt ggagttacac ttgacaaaga agctaatgga gttcgcggag aagagatcgt
     1141 tatctctaca ccggattcca aagttaaagt tgctgtaatt cctacaaacg aagaacttgc
     1201 aatctgcaga gagacagtag ctctggttta attttcacag ataataaagt gtctgttttg
     1261 tgtccaaacg agagaaatat aactggataa aagttatatt tatatagaag ggaagggtgc
     1321 atctgtcagg agcacccttc tttgtgcccg taaggcatat ggaaattacc tgataataat
     1381 gccgccggat gataaaatac ttcgtaaagg aatattactt gacaaaatgt gaaatttttc
     1441 taatttatcg ttgacaaaag cagaggcggt atgtataata aaacaagtgt ggataggaga
     1501 aaagtatgca gatacattta tcagatattt catccagtga aggaatgcgt attcagaaga
     1561 ctgcagagtt tggaatggat acgatcacct ttcagtcagg aagctttccg gtacttgcaa
     1621 aagaacccat agagcttacg atcacgaata caggtgacag aaatcttgaa atcagaggaa
     1681 cgggtaagat taccgtaggt attccctgtg acagatgtct ggaagaggtt tcaacagaga
     1741 ttcctttgga gattgagcga aaacttgata tgaagcttac ggatgaagac cgtgtgaatg
     1801 atctggacga gagttcttat ctcacaggta tggacctgga tgtggaccag ctggtctatc
     1861 ttgaggtact gatgagctgg cctttgaagg ttttatgtag agaagactgc aaaggaatct
     1921 gtagccagtg cgggaaaaac ctcaatgacg gtccctgtgg atgcgtcgag gaacctaagg
     1981 atccccgtat ggcagctatc agtgatatat ttagtaaatt taaggaggtg taacataatg
     2041 tccatctgtc cgaagaataa atcttccaaa gcaagacgcg ataagagaag agcaaactgg
     2101 aaaatgagcg ctccgaacct tgtaaaatgc agcaaatgtg gcgaactgat gatgccgcac
     2161 agagtatgca aagcttgcgg aagctacaac aaaaaggaaa tcattaaagt tgaagactaa
     2221 tttttaatga ttgcaaagta tggcaggttc tcccgtttgg gatgcctgct tttttgtgta
     2281 ttattttatg gatgaggaaa aagtaatgaa tgagaaggag aaggtaaatc agatcacaga
     2341 aggtgtgatc tggaaacagc tgctgttatt tttctttcct attgtatttg gaacattttt
     2401 tcagcagatt tataatacgg cggatactat tgttgtaggt cgtttcgtag gaaaacaggc
     2461 acttgctgca gttggaggtt ctgccagtca gatcgccaat ctgatcgttg gcttttttgt
     2521 agggctttct tcaggggcag cagttgtgat ctcgcagttt tatggggcaa aagataagaa
     2581 gaatctgagt aaggcactgc atacagcatt cgcattctcc attgtagcgg gaattgttct
     2641 tactgtagtg gggatttttc tgaccagacc tgcattaatg cttatgaaga cacctgcaga
     2701 tgtag
//
LOCUS       DBMDXG010000301         2699 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8261_length_2699_cov_1.274827, whole genome shotgun sequence.
ACCESSION   DBMDXG010000301 DBMDXG010000000
VERSION     DBMDXG010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8261_length_2699_cov_1.274827"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="ACZPD3_08205"
     CDS             <1..1658
                     /locus_tag="ACZPD3_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR1416004.1"
                     /translation="SKFSDANASDTEKNLYAKFQQKQQQVFDTITNRLTAENPPAVKD
                     EDDQIQEYLTYICDDLLRDTLGVISKNAIDTSDSTYQKWTTDKDISLKDYLTYAASQN
                     WIDISKFSTEGDYLDSDEVYQALTDYLIDYLKKDTNFSKLLYKYMLQEDTISGSEICL
                     VLYEQGVLSKDDGSYEALASGSMTAYDFMINKIYTLEIEPAQLALEPCSASAVITDVK
                     TGDVLACVSYPGYDNNRLVNNMDTDYYAKLSTDKSSPFFNKATQQTAAPGSTFKILST
                     IAGMSEGVIDDGTYINCTGSFDLVTPPINCWNKQGHGEIEIREAIEQSCNTYFNMVGF
                     KLGQDADGNFSENRSLSVLQKYASEIGLDKKTGIEITESAPHVSDSYAVPSYIGHGTN
                     AYTTSQLARYATAIATSGNVYDLTLLDRQTDSKGNTLKKYEPDIINTVDVSQNVWDDI
                     HDGMYRVVQTHRQFDGLGVDVAGKTGTAEVNIYHPNHGLFMGYAPASDPEYAIAVRIE
                     NGYTSGNACLAADDIFKYIFELTDEKSILTGVAASDTSDTSND"
     gene            1719..2507
                     /gene="minD"
                     /locus_tag="ACZPD3_08210"
     CDS             1719..2507
                     /gene="minD"
                     /locus_tag="ACZPD3_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR1416005.1"
                     /translation="MSEVIVITSGKGGVGKTTTVANIGTGLAMLGKKVVVVDTDIGLR
                     NLDVVLGLENRIVYNLVDVINGSCRLRQALIKDKRHPELCLLPSAQTKDKSAVSPEQM
                     IKLTDDLREQFDYILLDCPAGIEQGFKNAIAGADKALVVTTPEVSAIRDADRIIGLLE
                     ANEIRDISLIINRLRPDMIARGDMMSVDDVTDILAVDLIGTILDDEQIVIATNQGEPL
                     SGKSSQAEEEYMNICKRLLGEEIPFADINRKQGLLRKIGSFFKK"
     gene            2533..>2699
                     /gene="minE"
                     /locus_tag="ACZPD3_08215"
     CDS             2533..>2699
                     /gene="minE"
                     /locus_tag="ACZPD3_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR1416006.1"
                     /translation="MKAFFKEKKRSAGYARDRMKLLLISERIDCSPQMMKMLRNDMIH
                     TVKKYLTIDEE"
BASE COUNT          840 a          515 c          627 g          717 t
ORIGIN      
        1 tcagtaaatt cagtgatgcc aacgcctccg atacagaaaa aaatttatat gccaaatttc
       61 aacagaaaca gcaacaggtt tttgatacaa taactaacag gctgacagct gagaatccac
      121 cggccgtaaa agatgaagat gatcagatcc aggaatatct tacttatatc tgtgatgatc
      181 ttctcagaga tacgctgggg gttatctcaa agaatgccat tgatacctct gacagtactt
      241 atcagaaatg gacaaccgat aaagatatca gtctgaaaga ttatctgaca tatgcggcaa
      301 gtcagaactg gatcgatatt tccaaatttt ctacagaagg agattatctg gattccgatg
      361 aagtatatca ggctcttaca gattatctga tcgattatct taaaaaagac accaatttct
      421 caaaacttct gtataaatat atgcttcagg aggacaccat ttccggaagc gagatctgtc
      481 tggtgctgta tgaacaggga gttttatcaa aagatgatgg cagttacgag gctctggcct
      541 ccggctccat gacagcttat gactttatga tcaataaaat ttatactctc gaaatagaac
      601 ctgcacagct tgcgctggag ccatgttctg cttctgcagt aatcacagat gtcaaaacag
      661 gggatgttct tgcatgcgta tcctacccgg gatatgacaa taaccgtctg gtaaataaca
      721 tggatacgga ttattatgcg aaattatcta cagataaatc cagtccgttt ttcaataaag
      781 caacccaaca gacagcagca ccgggttcta catttaagat tctgtctaca attgccggaa
      841 tgtctgaagg agtgatcgat gacggaacat acattaactg taccggctcc tttgatcttg
      901 taacacctcc gatcaactgc tggaacaaac agggacatgg agagatcgag atcagggagg
      961 cgattgagca gtcctgtaac acttatttca acatggttgg ttttaaactt ggtcaggatg
     1021 cagacggaaa cttttcagaa aaccgaagcc tgagtgtact gcaaaaatat gcttcagaga
     1081 ttggcctgga taagaaaaca ggtatcgaga tcacagaatc cgcacctcat gtgtcagatt
     1141 cctacgcagt cccgtcttat atcggacatg gtaccaatgc ttataccacc agtcagctgg
     1201 cacgttatgc aacagcgatc gctaccagtg gaaatgttta tgatctgact ttacttgaca
     1261 gacagacaga ttccaagggt aatactttaa aaaaatatga gccggatatc attaatacag
     1321 ttgatgtatc acagaatgtg tgggatgata ttcacgacgg aatgtaccgg gtagtacaga
     1381 ctcacagaca gtttgacgga cttggcgtgg atgtggcagg taaaaccggt acagccgagg
     1441 taaatattta tcatccaaac catggtttgt ttatgggata tgccccggct tcagatcctg
     1501 aatatgcgat tgcagtgcgt attgagaatg gatatacttc cggtaatgcg tgtcttgcag
     1561 ctgatgatat ttttaaatat atctttgaat taactgatga aaagtctatt cttaccggtg
     1621 ttgcggcaag tgataccagt gacacttcga atgactgaaa ctaccataca ggagaatacc
     1681 agaagaatgg aaattttttc agggaggaaa ttgtaactat gagtgaagtc attgtgatta
     1741 cttccggcaa aggcggagta ggaaaaacca caactgtcgc caatatcgga acaggtctgg
     1801 ctatgctggg caaaaaagtt gttgtggttg atacagatat cggtctgcgg aatctggatg
     1861 ttgttctggg cctggagaac aggattgtct ataatctggt ggatgtcatt aacggaagct
     1921 gccgtctgcg acaggctctg atcaaggata aaagacatcc ggaattatgt cttctgccat
     1981 ctgcgcagac aaaagacaaa tctgctgtgt ctccggaaca gatgataaaa ctgaccgacg
     2041 atctgagaga acagtttgat tatattcttc tggactgtcc ggcaggaatt gaacagggat
     2101 ttaaaaatgc gattgcaggt gctgataaag ccctggtagt taccacgccg gaggtttctg
     2161 cgatacgtga tgcagatcgg atcatcggac tcctggaagc caacgagatc agagatatca
     2221 gcctgatcat caatcgcctg cggccggata tgattgcaag aggtgatatg atgtccgtag
     2281 atgatgtaac ggatatcctt gctgttgatc tgatcggaac aattctggat gatgaacaga
     2341 ttgtgattgc cacaaatcag ggggagcctc tttccgggaa atcttctcag gcagaagaag
     2401 aatacatgaa tatctgtaaa cgccttttag gtgaagagat tccgtttgca gacattaaca
     2461 ggaagcaggg gttattaaga aaaataggta gttttttcaa aaagtagcct gtatttataa
     2521 gggggaatct gtgtgaaagc tttttttaaa gaaaagaagc gttcagccgg atatgcaaga
     2581 gaccgcatga agcttctgct gatctcggaa cgtatcgact gctctccaca gatgatgaaa
     2641 atgttgcgga atgatatgat acataccgta aaaaaatacc ttactattga tgaagaaca
//
LOCUS       DBMDXG010000302         2695 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8269_length_2695_cov_2.273323, whole genome shotgun sequence.
ACCESSION   DBMDXG010000302 DBMDXG010000000
VERSION     DBMDXG010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8269_length_2695_cov_2.273323"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..317)
                     /locus_tag="ACZPD3_08220"
                     /pseudo
     CDS             complement(1..317)
                     /locus_tag="ACZPD3_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706950.1"
                     /note="Catalyzes the formation of PRPP from ATP and ribose
                     5-phosphate; frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
     gene            complement(321..1304)
                     /locus_tag="ACZPD3_08225"
     CDS             complement(321..1304)
                     /locus_tag="ACZPD3_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1416007.1"
                     /translation="MPLQNYQYDTIMREYSKTQSQNRRILEERTQEIYKKIPRIHEID
                     EEVATLSAKKARALLSGESSGLEDLKAAISLLSQERNALLVCNSYPEDYLELPYKCPV
                     CQDTGYVGSQKCTCFKKAEIELLYTQSNLKEILKKENFDHFSFDYYSDTMKNEATGLT
                     ERETARRAYDIARGFVRNFDSSFENLFLYGDTGVGKTFLSHCIAHDLLESAHCVMYFS
                     AFDLFELLADSKFSRDKTEGQEFVFDSDLLIIDDLGTELTNSFVSSQLFLCINERIMR
                     RKSTIISTNLKLENFSDTYSERTFSRIASNYRMVKLEGKDIRIQKIFLGGK"
     gene            complement(1373..2455)
                     /locus_tag="ACZPD3_08230"
     CDS             complement(1373..2455)
                     /locus_tag="ACZPD3_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR1416008.1"
                     /translation="MAMITLQNSRNAEVTVLTNNFIDNYMPGANGEFVKVYIYLLRLL
                     SDTSVPFSLEQMADHFFCTERDIIRALKYWEKEKLLTLTYRNNEDIADIILNVPPVKS
                     AASDTPVSAAPVTKTETQTSSAPAQPVKQTTRSATALSPDRVKELKQNDEIVQLLYIA
                     EQYLGKTLTPTEMKKILFFYDELKFSPDLIEYLIEYSVSRGHKSMRYIETVALAWADE
                     GITTVTMAKEANSRYAKEYFTIFKSMGISGRNPVDTEISLMNTWLNDYGFTMDIIQEA
                     CSRTVLSTGQPSFQYADKILSGWKDKNVRTLADVRLLDAQHQRQKLEKNTQRKAASKP
                     AASNRFNNFHQRQYDFNEYEKKLLNQ"
BASE COUNT          714 a          568 c          572 g          841 t
ORIGIN      
        1 cgtatggtcc gatgctgtag gtaagactgt agttatacat caaccatcag atagatatcg
       61 tcaccacgta cagattccat gatcgtacat ttcgcctcac cggaaccgaa acgtgctgtc
      121 tggaccggta tcagatagga atctctctga taaccctcaa atgcaaaaga atcaagctct
      181 tcgtggctgc gctctttacg ccactgtatc agccattcat ttactttttc acccagatcc
      241 ttacagctga tcagagggat cagaccaagt ctgcccaccg gaagtgtagt taaatcttta
      301 gttgttgcct gtgccatgtt ttattttcct cctaaaaaaa ttttctgtat acggatatcc
      361 tttccttcca gcttcaccat acggtagttg ctggcgattc tcgaaaaggt tcgttcggaa
      421 taagtatccg agaaattttc cagtttcaga tttgttgata tgatcgtaga cttcctgcgc
      481 atgatgcgtt catttatgca caaaaacaac tgggaagaga caaagctgtt tgtaagttca
      541 gttcccaggt cgtcgatgat cagcagatcg ctgtcaaata caaattcctg tccttctgtt
      601 ttatctctgg agaatttgga gtctgccagc agttcaaaca gatcaaatgc ggaaaagtac
      661 ataacgcaat gtgctgattc cagaagatca tgagcaatac agtgagaaag aaaagtctta
      721 cccacaccgg tatcaccgta aagaaacaga ttctcaaagg aactgtcaaa atttctcaca
      781 aaacctctgg caatatcata agctctccgg gccgtctctc tttctgtcag tccggtagct
      841 tcgttcttca ttgtatcaga ataataatca aaagaaaagt ggtcaaagtt ttcttttttg
      901 agaatttctt ttaaattgga ctgcgtatag agcagttcta tctctgcttt cttaaaacag
      961 gtgcattttt ggcttccgac gtaaccggta tcctgacaga ccggacactt atatggaagt
     1021 tccagataat cctccggata tgagttacat accagcaggg cattgcgctc ctgggataga
     1081 agagagattg cagctttaag gtcctcaaga cctgatgatt ctccgcttaa gagtgctctt
     1141 gcttttttgg cgcttaaggt ggcaacctct tcatctattt cgtggatgcg cgggattttt
     1201 ttataaatct cctgagtacg ctcctccaga atacgtctgt tctgagactg ggtcttgctg
     1261 tattcccgca tgatcgtatc atactgataa ttctgtaaag gcatgacttc acaccctttc
     1321 gtttgttttt ttgtagctgt tcagtgactt tcagacgatt atgtcactgc cgttactgat
     1381 tcagtaattt cttttcatat tcgttaaagt catactgtcg ctgatggaaa ttgttaaacc
     1441 ggtttgatgc agccggtttg gaagctgctt ttcgctgggt attcttttcc agcttctgtc
     1501 gctgatgctg agcatcgagg agccggacat ctgcaagtgt acggacattt ttatctttcc
     1561 atccggaaag gattttatct gcatactgga aacttggctg tccggtagag agaactgtac
     1621 ggctgcaggc ttcctggatg atatccatgg taaaaccata atcattcagc caggtgttca
     1681 taagagaaat ctctgtatct accggatttc gtccgctgat tcccattgat ttaaaaatag
     1741 taaagtattc tttggcataa cgggaatttg cctcttttgc catggtgaca gtggtgattc
     1801 cctcatctgc ccaggcaaga gcaacggttt ctatgtaacg catactctta tgcccgcggc
     1861 tgacactgta ttcgatcagg tactcgatca gatcaggcga gaatttcagc tcatcataga
     1921 aaaacaggat ctttttcatc tcggtaggag tgagcgtttt gccgagatac tgttctgcga
     1981 tatagaggag ctgtacgatc tcatcatttt gctttaattc ttttacacgg tcaggagaca
     2041 gtgcagtggc tgatctggtg gtctgcttca caggctgtgc cggcgcagaa gaagtctggg
     2101 tttctgtttt tgtaacaggg gctgccgaaa caggtgtgtc ggaagcagca gattttacag
     2161 gtggtacatt caatatgata tctgctatat cttcgttatt tctgtaggtt aaagtaagga
     2221 gtttttcttt ctcccagtat ttcagagcgc ggataatgtc acgttctgta cagaagaaat
     2281 gatcagccat ctgctccaga ctgaagggta cagaggtgtc agaaagaagc ctcagaagat
     2341 agatatatac tttcacaaat tctccattgg ccccaggcat atagttgtct atgaaattgt
     2401 tggttaatac agttacttcc gcattgcggg aattctgtaa agtgatcata gccatgaatc
     2461 tccactctct ttcataataa taagatgtta gggtcaaaag gtattttgcc cctaacctga
     2521 tatccacgaa ttgctccgct gcaaagcagc tcccacaatt ctcataataa taagatgtca
     2581 gagtaaaaaa agatattttt cctctgactt gatatctatg aattatagca caatattttt
     2641 taaaagagaa taggggattt ttgtcgaaga tttcatccac atttgaatgt ggata
//
LOCUS       DBMDXG010000303         2695 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8271_length_2695_cov_1.814572, whole genome shotgun sequence.
ACCESSION   DBMDXG010000303 DBMDXG010000000
VERSION     DBMDXG010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8271_length_2695_cov_1.814572"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="ACZPD3_08235"
     CDS             complement(<1..1323)
                     /locus_tag="ACZPD3_08235"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR1416009.1"
                     /translation="MSKFLVIAEKPSVAQSYAKNLSAYKREDGYLEGESCIVSWCLGH
                     LAEYAQPEEYDPKYEKWQFDDLPILPEAWKLKVSKDKKKQFDVLKGLMTRSDVEYLVN
                     GCDAGREGELIFQRVYVLAGCRKPVKRLWISSMEDAAIQKGFQTMKSEEEYKNLCMAA
                     VCRAQADWLIGMNGTRAYTTRYFKRLVVGRVQTPTLAMIAERQERIEHFQKEAFYKVA
                     LTDGKLTVVSENIANEEAADLLAALCNGSTAVVTQMKKEDKKAFPPRLYDLTSLQREA
                     NRYFGYTAKRTLEMLQELYEEKLVTYPRTDSQFVTEDMKDSVEELVGKMPVLLSFVDY
                     GQLGHGIKRVINNAKVSDHHAILPTKEAVEKGISDLPADKKNLMMLICQQLVQATGEE
                     YLYEQTDITVKCQKHDFKARGKIPVQMGFKEVEKVFKHLCVKAEPVEEK"
     gene            complement(1406..2089)
                     /locus_tag="ACZPD3_08240"
     CDS             complement(1406..2089)
                     /locus_tag="ACZPD3_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HIR1416010.1"
                     /translation="MRNKVVKKLLMIAMAVTMISGTSLATVYANANPPAEETTTAVVE
                     ETTTEKSKDTEKAEDTGRVTGESNNSAFSTPGNAQLVDDKENDDTKQFLTIQTKKGNT
                     FYMVIDRSSNTENVYMMSLVDENDLAEFLDETEKEKETEESTVVIPETTPETTPVAEE
                     ETEVKKEEKSGMNVKGLAAIVVAAILGFAGIAVYKKHGRSKDEDYVSEQLEFSDGPYV
                     NEEDDEKEE"
     gene            complement(2079..2408)
                     /locus_tag="ACZPD3_08245"
     CDS             complement(2079..2408)
                     /locus_tag="ACZPD3_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HIR1416011.1"
                     /translation="MNMKLKKILDDIQKTEDKIAELQEHLKRQNTLKQQMEDMEIIKS
                     FRSMKLDSRELLVLLDGIQKGTVTIQMNEDGGITVEDTKETPQKESDNRPPVGQVAER
                     EVLDDEE"
     gene            complement(2426..>2695)
                     /locus_tag="ACZPD3_08250"
     CDS             complement(2426..>2695)
                     /locus_tag="ACZPD3_08250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR1416012.1"
                     /translation="WVINNCGNGWNVGRCTADELRSHCSQVSPSEAKPGDLIFFQGTY
                     STPGASHVGIYVGNNMMIHCGKPVQYTSIASAYWQEHFMAFGRLH"
BASE COUNT          600 a          723 c          440 g          932 t
ORIGIN      
        1 cttttcttcc actggttccg ctttgacaca cagatgttta aatacctttt ctacttcctt
       61 aaatcccatc tgtactggaa tcttccctct ggctttaaaa tcatgtttct ggcactttac
      121 cgtaatatct gtctgctcat acagatattc ttctccggtt gcctgtacca gctgctgaca
      181 gatcaacatc atcagattct ttttatctgc cggtaaatca gagattcctt tttctacagc
      241 ttctttcgtc ggaagaatcg catggtgatc agataccttt gcattgttga ttaccctttt
      301 tatgccatgc cccaactgtc cataatctac aaacgaaagt aatactggca tttttcctac
      361 cagctcttcc accgaatctt tcatatcctc cgttacaaac tggctgtctg ttcttggata
      421 agtaaccagc ttttcttcat ataattcctg taacatctcc agtgtgcgtt ttgctgtata
      481 tccaaagtaa cggttggctt ctctctgcag gcttgtcaaa tcataaagtc ttggcggaaa
      541 tgctttttta tcctccttct tcatctgtgt gaccacagcg gtcgaaccat tacataacgc
      601 tgccagcaga tccgcagctt cttcatttgc aatgttttcg gaaactactg taagttttcc
      661 atctgtcagt gctaccttat aaaacgcttc tttttgaaaa tgctcaatcc tctcctgccg
      721 ttctgcaatc atggcaaggg ttggggtctg taccctgcct acaaccaatc gtttaaaata
      781 tctcgtggtg tatgctctgg ttccattcat tccgatcagc cagtcagcct gtgccctgca
      841 caccgcagcc atacagagat tcttgtattc ttcctcactt ttcattgtct ggaagccttt
      901 ctgaatggcg gcatcttcca ttgagctgat ccacagacgt tttactggtt tcctgcatcc
      961 ggcaagcaca tacacccttt gaaagatcag ttctccttct cttcctgcat cacagccatt
     1021 taccagatat tccacatcgc ttcgggtcat cagacctttt aagacatcaa actgcttctt
     1081 tttatccttg gaaactttca acttccatgc ttctggaagg attggcagat catcgaactg
     1141 ccatttttca tatttcgggt catattcctc tggctgtgca tactctgcca gatgtcctaa
     1201 gcaccagctg acgatacagc tttctccctc cagataacca tcctcccgct tataagcgga
     1261 cagatttttc gcatagcttt gtgctacact tggcttctct gcgatcacta aaaatttaga
     1321 cataatcctt tcctttctat caaaaaggca gccaaaattt ctggctgccc ctgtccttat
     1381 tactgtttcc atctcttaaa aacgcttatt cttctttttc atcgtcctct tcatttacat
     1441 acggaccatc tgaaaattca agctgttcac ttacataatc ttcgtcctta ctacgtccat
     1501 gtttcttata gactgcgatt cctgcaaagc ctaagattgc tgcaactacg atagctgcaa
     1561 gacctttcac attcattccg cttttctctt ctttcttcac ttcggtttcc tcctctgcta
     1621 ctggtgtagt ctccggtgtg gtttccggga tcactacggt tgattcttca gtttccttct
     1681 ctttttctgt ttcatccaga aattctgcca gatcattctc atcgaccaga gacatcatat
     1741 acacgttctc tgtattactg gaacggtcaa tgaccatgta aaaagtattc ccctttttcg
     1801 tctggatagt aaggaactgt tttgtatcat cattttcctt atcatctaca agctgggcat
     1861 ttcccggtgt agaaaaagca gaattattac tctctccggt aactcttccc gtatcctctg
     1921 ctttctccgt atctttactt ttttctgttg ttgtctcttc caccaccgct gtcgttgtct
     1981 cttctgccgg tggatttgca tttgcataaa cagtagcaag ggatgttccc gaaatcattg
     2041 ttaccgccat agcaatcatc agaagtttct ttaccacttt attcctcatc gtctaaaacc
     2101 tctctttctg ccacctgccc cacaggtggt ctgttatcgc tttctttttg gggtgtctcc
     2161 ttggtatctt ccactgtgat accgccgtcc tcattcatct gaatcgtaac ggttcctttc
     2221 tgaatcccgt caagcagtac cagcagctcc cgactgtcca gcttcataga acgaaatgat
     2281 ttgataatct ccatatcttc catctgctgt tttaaagtat tctgccgttt cagatgttcc
     2341 tgaagctctg caatcttatc ctctgttttc tgaatatcat ccagaatctt ttttaatttc
     2401 atgttcatct gctctgtcac ctcctctaat gcagacgacc aaatgccata aagtgttcct
     2461 gccagtaggc acttgcgata cttgtatatt gcaccggctt accacaatgg atcatcatgt
     2521 tatttccaac ataaattccc acatggcttg ctcccggtgt actgtatgta ccctggaaga
     2581 agatcaggtc tcccggtttt gcttctgatg gagatacctg cgaacagtgc gaacgaagtt
     2641 catctgctgt acatcttccg acattccagc catttccaca gttattgata accca
//
LOCUS       DBMDXG010000304         2695 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8274_length_2695_cov_1.331920, whole genome shotgun sequence.
ACCESSION   DBMDXG010000304 DBMDXG010000000
VERSION     DBMDXG010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8274_length_2695_cov_1.331920"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="ACZPD3_08255"
     CDS             <1..561
                     /locus_tag="ACZPD3_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416013.1"
                     /translation="LRIPTPNFIVFYNGTEKKEDRWVDYLSESYENMSGEPNLELKVI
                     TLNINVGHNKKLMEECRTLREYAQYVDKVRRYSNEMELNTAVERAVSESIQEGILKEF
                     LQKNRAEVVAMSIFEYNEEEEKRKLRKAEYEAGMAEGVMKTKKETAISLAEMGLSVQQ
                     IAQGVKVEEKTVHKWLNEKKNMKTTY"
     gene            757..>2695
                     /locus_tag="ACZPD3_08260"
     CDS             757..>2695
                     /locus_tag="ACZPD3_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR1416014.1"
                     /translation="MEKLVIGILAHVDAGKTTLSEGILYLTGKIRKLGRVDHKDAYLD
                     TYNLERERGITIFSKQAEFELGNRGITLLDTPGHVDFSAEMERTLQVLDYAILVINGA
                     DGVQGHTMTLWRLLARYQIPTFLFINKMDQDGTDKEKLLAELKKRLSDNCADFTWENT
                     SGIENSTDETAEKAEDEISDLQSRFLEDISVCDEELLEKYLETEEISTSDIRKVIKER
                     KLFPCFFGSALKMTGVEEFLHGLEKYCETPEYPSEFGAKIFKIARDDQGNRLSYMKIT
                     GGTLKVKELLTDTEKADQIRIYSGAKFELAKEAPAGTICAVTGLSQTHPGQGFGIERE
                     SEMPVLEPVLNYRILLPEDCDVHQMLKKLKELEEEEPELHIVWNEQLGEIHAMLMGEV
                     QIEILKHLIWERFHVAVEFGTGNIVYKETIAEPVEGVGHFEPLRHYAEVHLLLEPGEP
                     GSGLQFFTACSEDVLDRNWQRLILTHLEEREHPGVLTGSPITDMQITLITGRAHLKHT
                     EGGDFRQATYRAVRQGLKKAKSVLLEPYYEFRLEIPGDMIGRAMTDIQKMNGTFQQPE
                     ADEDDMMVLKGSAPVSMMRDYQTQVTSYTKGRGRLFCSLKGYAPCQNQDEIVEEIGYD
                     SERDLDNPTGSVFCAHGAGFVV"
BASE COUNT          895 a          452 c          699 g          649 t
ORIGIN      
        1 ctgagaatac ccacaccgaa ttttatcgta ttttataatg gaacagagaa aaaagaagat
       61 aggtgggtag attatctgtc agaatcgtat gaaaatatgt caggagaacc aaatcttgaa
      121 ctaaaggtca tcacattaaa tatcaatgta gggcataata aaaaactaat ggaagaatgc
      181 cggacactaa gagaatatgc tcagtatgtt gacaaagtac gcagatattc taatgaaatg
      241 gaactgaata cggcagtaga acgtgcagtg agcgaaagca tccaggaggg aattctcaaa
      301 gaatttctgc aaaagaatcg agcggaggta gttgctatga gtatatttga atacaatgag
      361 gaagaggaaa aaaggaaact caggaaagct gagtatgaag ctggtatggc agaaggagta
      421 atgaaaacta aaaaggaaac agcaatttca ttggctgaaa tgggattatc tgtgcagcag
      481 attgctcagg gagtaaaagt agaagaaaag actgtacata agtggctgaa tgaaaaaaag
      541 aatatgaaaa ccacatatta aaaataatgg aagcggagtc cacattaact gtaaatgtgg
      601 gctcttttgg actaaatgaa cacataaaaa cacaacccta ttggatttat ctatatcagt
      661 atgttaaaat aattaattga taaaggattt taacagatga agcgataaaa gaataactac
      721 tgattttata aagaaaaagg agtttttaaa tatactatgg agaaacttgt tattggtatc
      781 cttgcccacg tagatgcagg taagactacg ttgtcggagg ggattttata tttaacggga
      841 aaaatccgta agctggggcg tgtggatcat aaggatgcgt atcttgacac ctataacctg
      901 gagcgggagc gtggaattac gattttttcc aagcaggcgg agtttgaatt gggaaacagg
      961 gggataactc tgctagatac gccggggcat gtggatttct cggcggaaat ggagaggaca
     1021 ctgcaggttc tggattatgc gattcttgtg ataaatggag cggatggtgt gcaggggcat
     1081 actatgacct tatggagact tctggcaaga taccagattc cgacgttcct gtttatcaat
     1141 aaaatggatc aggacggaac tgataaagag aaacttctgg cagaattaaa gaagcgatta
     1201 agtgataatt gcgctgattt cacctgggaa aacacaagtg gaatagaaaa tagtactgac
     1261 gaaacagcag agaaagcaga agatgaaata tcagatctgc agtctcggtt tctggaagat
     1321 atttccgtct gcgatgaaga actgcttgaa aaatatctgg aaacagagga aatatcaaca
     1381 tcagatatcc gaaaagtaat caaagagcga aaacttttcc catgtttctt tggctctgcg
     1441 ctgaaaatga caggtgtaga agaattcctc catggtttgg aaaaatactg tgagacacca
     1501 gaatatccat ccgagtttgg cgcaaaaata ttcaagattg ccagagacga tcagggcaat
     1561 cgtttaagct atatgaaaat cacaggtgga acattgaagg tgaaggaact tctcacagat
     1621 acagagaagg cagatcagat ccgtatctat tccggtgcga aatttgagct tgcaaaggag
     1681 gctcctgcag gaaccatctg tgcagtaacc ggtctttccc agacacatcc gggacagggc
     1741 tttgggattg aacgggaatc cgaaatgccg gttctggaac ctgtattaaa ttacaggatt
     1801 ctgcttcccg aggactgtga tgttcaccag atgctgaaga aactcaaaga gctggaagag
     1861 gaggaaccgg aacttcacat tgtatggaac gaacagcttg gagaaatcca tgccatgctg
     1921 atgggtgagg ttcagatcga gatattaaag catctgatct gggagcgttt ccatgtagca
     1981 gtagagtttg gcacaggaaa tatcgtatat aaagaaacaa tcgcagagcc ggtagaaggt
     2041 gtaggccatt ttgaaccgct gcgtcattat gcagaagtac atctgcttct ggaaccggga
     2101 gagccgggga gcggtctgca gttttttact gcatgcagtg aggacgtgct ggaccgtaac
     2161 tggcagcgtc tgatcctgac tcatctggaa gaaagggagc acccgggagt actgaccggt
     2221 tctccaatta cagatatgca gattacactg attacaggac gcgcccatct gaaacacaca
     2281 gagggtggcg atttccggca ggcaacgtac agggctgtgc gtcagggatt aaagaaagca
     2341 aaaagtgtac tgcttgagcc atattatgag ttcagacttg agattccggg ggatatgata
     2401 gggcgtgcca tgactgacat tcagaaaatg aatggaactt tccagcagcc ggaagcagat
     2461 gaggatgata tgatggttct gaaaggcagt gcacctgtat caatgatgcg agattatcag
     2521 acacaggtaa cctcctatac aaagggacgg ggcagattat tctgttcatt gaagggctat
     2581 gccccatgtc agaatcagga tgaaattgta gaggagattg gatatgattc cgaacgtgac
     2641 ttggacaatc ccacgggttc tgtattctgt gctcatggag caggatttgt ggtgc
//
LOCUS       DBMDXG010000305         2686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8314_length_2686_cov_1.258414, whole genome shotgun sequence.
ACCESSION   DBMDXG010000305 DBMDXG010000000
VERSION     DBMDXG010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8314_length_2686_cov_1.258414"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(3..779)
                     /gene="cbiQ"
                     /locus_tag="ACZPD3_08265"
     CDS             complement(3..779)
                     /gene="cbiQ"
                     /locus_tag="ACZPD3_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HIR1416015.1"
                     /translation="MIVIDKLCYQSKLRYENAGEKFAFAVITLCICVMSRSAAVAGIV
                     LAATGILTVFYGGVPVFRYLKFLTAPLAFLLLSTVAIILNIRKTPLDLFAIPVGTWYL
                     TSSLDSCLYALQLILTALAAVSCLYFLSFTTPVPDILDVLKKLHCPKILIELMLLIYR
                     FIFVLLETASAISMSQDCRLGNKNYRTALKSFGMMGSVLMIRAISRSNRLYDAMEARC
                     YDGTIHVLSESRPPKKKVILAIILFDGALFLFALWRRFYL"
     gene            complement(816..1115)
                     /locus_tag="ACZPD3_08270"
     CDS             complement(816..1115)
                     /locus_tag="ACZPD3_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR1416016.1"
                     /translation="MKKTVIAAIVAIFLIAFVPLFVLKDAEFGGSDDAGSVVVEEVDS
                     SYEPWATPVFEQLLGGELPGEVESLLFCIQTGIGVGIIAFIMGRFVERKKWMKDQ"
     gene            complement(1117..1863)
                     /locus_tag="ACZPD3_08275"
     CDS             complement(1117..1863)
                     /locus_tag="ACZPD3_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR1416017.1"
                     /translation="MSKKQKKILVLAGAFALAFGIVPNVSAMHIMEGYLPGSFCIAWG
                     VLCVPFLIAGFMSIKKTLNEHRNLITMLAMSGAFIFVISSLKIPSVTGSCSHMTGTGL
                     GAILFGPAAVSILGLIVLLFQAILLAHGGLTTLGANTFSMAIAGPFVSYGIYVLCKKM
                     NVNKRVAVFLAAALGDLFTYCVTAGQLALAHNADTTFGAALGKFMAIFAPTQIPLAII
                     EGIITVVIFMGLETYAKPELRAMGILKEAK"
     regulatory      complement(2019..2210)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2456..>2686
                     /locus_tag="ACZPD3_08280"
     CDS             2456..>2686
                     /locus_tag="ACZPD3_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR1416018.1"
                     /translation="MAEILVCDDDKAIVEAIEIYLSQEGHHILKAYDGEEALRTLREN
                     PVDLLIIDVMMPKLDGIRATLKIREENPLPIII"
BASE COUNT          831 a          598 c          605 g          652 t
ORIGIN      
        1 attcataaat aaaatctcct ccacagagca aataaaaata atgcgccgtc aaacaggata
       61 atagccagaa tcaccttttt tttaggcggg cgggattctg acagaacatg gatagttccg
      121 tcataacacc gggcttccat tgcgtcataa aggcggttgg agcggctgat ggcgcgtatc
      181 atcagaacag accccatcat accgaaggac ttcagagctg tccggtagtt tttatttcca
      241 agcctgcagt cctgagacat ggaaatcgcg ctggcagttt ccagcagaac aaaaataaaa
      301 cgatagatca gaagcataag ctcgatcagt attttgggac agtgcagttt ctttaacaca
      361 tccaggatat ccggtaccgg agtggtaaag gaaagaaagt acaggcagga aaccgctgca
      421 agtgcagtaa ggatcagctg cagggcatac aggcaggaat ccagactgga ggtaagatac
      481 catgttccca caggaatggc gaacagatcc agaggagttt tccggatatt cagtataata
      541 gccacagtgc ttaaaagtaa aaaggccagt ggggcagtaa ggaacttcag atagcgaaaa
      601 acagggaccc caccataaaa aacggtaaga atccctgttg ctgctaaaac aatccctgcc
      661 actgccgcgg aacgactcat tacgcagata cacagcgtaa tcacagcgaa ggcaaatttt
      721 tcaccggcat tttcatatct aagctttgac tgataacaaa gcttgtcaat gacaatcata
      781 aaaatctcct ttcatcagag aaggccggat tttgatcatt gatccttcat ccattttttg
      841 cgttctacaa aacgtcccat tataaaggcg ataattccga ctccgatacc tgtctggata
      901 cagaaaagga gactttccac ctcgcccgga agctctccgc caagaagctg ttcaaaaaca
      961 ggcgtagccc atggctcata agaggaatcg acttcttcca caacaacact tccggcatcg
     1021 tcagaaccgc cgaactcggc atccttcagt acaaacagcg ggacaaaagc aatcagaaaa
     1081 atggcaacga ttgcagcgat tacagtcttt ttcatattat tttgcctcct tcagaattcc
     1141 cattgcgcga agctccggtt ttgcataggt ttcaagaccc atgaaaatta ctacagtgat
     1201 gataccttcg ataatggcaa gaggtatctg tgttggtgcg aaaatagcca tgaatttgcc
     1261 cagggcagct ccgaaggtag tgtctgcgtt atgagcaaga gcaagctggc cggctgtgac
     1321 gcagtaggta aacagatcgc ccagagcagc cgcaaggaaa acagcaaccc gtttatttac
     1381 attcattttt ttgcacagaa catagattcc ataagaaaca aacggaccgg caatagccat
     1441 ggaaaaggtg tttgctccaa gtgtggtaag tcctccgtgt gccagaagaa tcgcctggaa
     1501 cagaagaacg atcagtccca gaatggaaac agcggccgga ccgaagagaa tggctccgag
     1561 accggttccg gtcatatgtg agcagcttcc tgtgacggac ggtatcttca gtgaagaaat
     1621 cacaaagata aaggcgccgg acatggccag catggtgatc aggtttctgt gttcattcag
     1681 agtctttttt atggacataa atcctgcaat aagaaagggt acgcataaaa caccccatgc
     1741 gatgcaaaag cttcccggaa gataaccctc catgatatgc atggcactga cgtttggaac
     1801 aatgccgaaa gcaagagcaa aggctccggc aagtacaaga attttctttt gttttttact
     1861 cattttcttc tcccttctga tcaaacagaa ctaaaataaa aaaacctgtg cggttatacc
     1921 gacaggccat caggagaaca agaggtaata gcaggaaaat agaattgatc tgctatgagc
     1981 caacctgttc ttacacaagg ccatcgttcg taacctgtgt aaatttcatg taaaaggcag
     2041 gccttctgac ttgggcatca tcactgcatt tcgtcttccc gggtatcttc agatattcag
     2101 tgacataatg aaatgcggct cttccgttac agcggcgtga ccgtgtgaga ctttcactca
     2161 actttcctat tctcctgcat aagcagacac cgttttacat tcaattctga atttattgta
     2221 gcataaaccc cagtgttgtc aaggaaaaac gcagcaggga tgcaggaaac cgcagcggtc
     2281 tttttttctg gatgacacta tgatatactg gttcaggaat gaaaaaaaca taagaaattc
     2341 ttaataattt acccctttta tcttaaacac catttgatat tcttagtata acgaatcaca
     2401 gaaaagagac tgtgatccgg tgtgaattta attttgtttc aggaggtaca gagacgtggc
     2461 agaaatactg gtttgtgatg atgacaaggc gatcgtggag gcaatagaga tctatctttc
     2521 acaggaaggt catcatattc ttaaagctta tgacggagag gaggctcttc ggacactgcg
     2581 ggaaaatccg gtagatctgc tgatcattga cgttatgatg ccaaagctgg acggaatccg
     2641 ggcgactctt aagatcagag aggaaaaccc gcttcccatt attatt
//
LOCUS       DBMDXG010000306         2678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8345_length_2678_cov_1.475747, whole genome shotgun sequence.
ACCESSION   DBMDXG010000306 DBMDXG010000000
VERSION     DBMDXG010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8345_length_2678_cov_1.475747"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            208..1125
                     /locus_tag="ACZPD3_08285"
     CDS             208..1125
                     /locus_tag="ACZPD3_08285"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR1416019.1"
                     /translation="MDTVVSETLYTTGEDITADVISALKDVEENWISWTKESSQIYQI
                     NQNAGNTTTVSDETATCLKQVLDLSKASDGAMDPTMGRVIRLWDIDGEDPHIPSDDEL
                     NSLLENVGYDKVTLDGNKVTMPEGVTLDLGAAGKGIGCDAAQKILDADKNVSGMILNL
                     GGSSVMSYGSKPDGSAWQVAVTDPRDTEGDYLGVVTLNGTEFLSTSGDYEKYFIEDGV
                     RYHHILNPATGYPARSGLTSVTVVCDDGLNADGLSTACFVLGKEKAEELLKKYNADGL
                     FVDDSDHVWMTEGMKERFQLLKDTYSIGE"
     gene            1376..2629
                     /locus_tag="ACZPD3_08290"
     CDS             1376..2629
                     /locus_tag="ACZPD3_08290"
                     /inference="COORDINATES: protein motif:HMM:NF026239.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding Tp47 domain C-containing
                     protein"
                     /protein_id="HIR1416020.1"
                     /translation="MRKGNLIKMAAPAILSAAMMVSGLPVMAADFTSDIAVETEAQTD
                     EFDAADDTADAFAGDEETPFVDDQTAAEDEFAATAETGYKYVYAGLTWAQYWASEGVY
                     NAANTASNATKDSHDELDKGGFDTVTRATVNHGLHRGSYQCEAIMYDKNGGSYEISYW
                     TSANDAVLTDGTTVKLNQPERGQITKADGSVVEFDHYNVVGLKYVPVAVKAEDYEAFK
                     SQFKVVEDGSTVAGGFGENNLQSYSAVADVTAETNGLKEAVKGSDGTFTFKARTNGTG
                     SGLKDQALQTASNVTATVKEASGSYGEFLRVDITGDGYGALGAKMYAVRWDYYGNGDK
                     VLASYGTKFAADNWMHKAMGIQLGLTDSYRCQLPKGTDGTGKWKITVYAMGYADTVFE
                     VNATDANIVKPEAGEADTTALKPPR"
BASE COUNT          864 a          503 c          643 g          668 t
ORIGIN      
        1 actatattgg tagacatact atccgtacag tgatatagtt tatagagtta gttgataata
       61 acaataagag gataaggaat cagaaaatat atgacaaaca gacactcttt tgtactggga
      121 ctttttatta cagcctccct tttatgcgca tcccggactg tatggggaaa ctctgaaata
      181 aacacataca ccaaaacaga ctttgctatg gacaccgtag tatcagagac tctttacaca
      241 acaggtgagg acatcacagc agacgtaatc tctgcattaa aagatgtgga agaaaactgg
      301 atctcctgga caaaagaaag ttctcagata taccagatca atcaaaatgc cgggaataca
      361 acaacggtat ctgatgaaac tgccacctgt ttaaaacagg tactggattt gtcgaaagct
      421 tctgacggag ccatggatcc tactatggga agagtgatcc gcctgtggga cattgacgga
      481 gaagatccgc acattccttc agatgatgaa ttaaattccc tgcttgaaaa tgtaggatat
      541 gacaaggtga ctctggatgg aaataaagtt accatgccgg agggagtaac ccttgatctg
      601 ggggctgcag gtaagggaat cggctgtgat gcagcacaga agattcttga tgcagataaa
      661 aatgtatcag gcatgatcct gaatctgggc ggcagcagtg ttatgagcta tggaagcaaa
      721 cccgacggct ctgcctggca ggtagctgtc actgatccgc gggatacaga aggtgattac
      781 ctgggagtag ttactctgaa tggtactgaa tttttatcta cttccggaga ttacgaaaaa
      841 tactttatag aagatggtgt tcgatatcat catatactga atcctgctac aggatatcct
      901 gcaagaagcg gtctcacatc tgtgacggtt gtgtgtgatg atggcctgaa cgcagacgga
      961 ctttccactg catgctttgt actgggaaaa gaaaaggcag aagaactttt gaagaaatac
     1021 aatgcagacg gactgttcgt agatgattct gatcatgtct ggatgacgga aggcatgaaa
     1081 gaaagatttc aattactgaa agatacttat tccatcggtg aataggttta tcttatataa
     1141 agatttctga actgtcaaaa atttcagttt tccggacagt ttggaaatat atttattgtt
     1201 actgttcact ccgttcacag taacatagcc aaaattcatt ccagattgcc tgcggcaatg
     1261 gaattttggc ttgtatgtct cgggattttg gcatattcat gccaaaacac ctcgcgggat
     1321 agtggtgtgt gaacagtaac tatttattaa ctcatattca ggaggattta aaggtatgcg
     1381 taagggaaat ttaatcaaaa tggcagctcc tgcaatttta agtgcagcaa tgatggtttc
     1441 aggattaccg gttatggctg cggattttac atctgatatt gctgtggaga ctgaggcaca
     1501 gacagatgag tttgatgcag cagatgacac cgctgatgct tttgcgggcg atgaggaaac
     1561 accttttgtt gacgaccaga cagcagcaga agatgagttc gctgcaacag cagaaacagg
     1621 ctacaaatat gtctatgcag gtctgacatg ggcacagtac tgggcatctg agggagttta
     1681 caatgctgca aacacagctt ccaatgcaac aaaagacagc catgatgaac ttgacaaggg
     1741 cggtttcgac acagtaacca gagctactgt aaaccatggc ctgcacagag gaagctatca
     1801 gtgtgaagca atcatgtatg acaaaaacgg cggttcttat gagatttcct actggacctc
     1861 agctaatgat gcagttctta cagacggaac aactgtaaag ctgaatcagc ctgagagagg
     1921 acagatcaca aaagctgacg gaagtgttgt agaattcgac cattacaacg tagtaggcct
     1981 gaaatatgta cctgtcgcag taaaagcaga agactatgaa gctttcaaat cccagttcaa
     2041 ggttgtggaa gacggaagca cagttgccgg aggatttgga gaaaataatt tacagtccta
     2101 tagtgcagtt gctgatgtta ctgctgaaac aaacggctta aaagaagctg taaaaggttc
     2161 cgatggaaca ttcacattta aggcaagaac aaacggtaca ggttccggac ttaaagatca
     2221 ggcactccag acagcatcca atgttaccgc aacagtaaaa gaagcaagtg gaagttacgg
     2281 cgaattctta agagtagata ttaccggtga cggatatgga gctcttggtg ctaaaatgta
     2341 tgctgtaaga tgggattact acggaaatgg tgataaagtt ctggcatctt acggaacaaa
     2401 atttgcagca gataactgga tgcacaaggc aatgggaatc cagcttggtc tgacagattc
     2461 ctatcgttgc cagcttccaa aaggaacaga tggaaccggc aaatggaaaa taaccgtata
     2521 tgcaatggga tatgcagata ctgtatttga agttaatgca acagatgcaa acatcgtaaa
     2581 accggaagca ggagaagcag atacaacagc attaaaacca ccacggtgaa atactcccta
     2641 ctgtcagcag cgctgccgct gctactacag taaaaata
//
LOCUS       DBMDXG010000307         2667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8379_length_2667_cov_1.815666, whole genome shotgun sequence.
ACCESSION   DBMDXG010000307 DBMDXG010000000
VERSION     DBMDXG010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8379_length_2667_cov_1.815666"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="ACZPD3_08295"
     CDS             <1..293
                     /locus_tag="ACZPD3_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1416021.1"
                     /translation="ILGYTNGEISRLYILSTSMVVVLCLLVSLPIETAVMKVLFREMM
                     LSSISGWITLWIDPMIYVQMFAAGIITYGIVALLEFRRVKKVPMDEALKNVE"
     gene            402..833
                     /locus_tag="ACZPD3_08300"
     CDS             402..833
                     /locus_tag="ACZPD3_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HIR1416022.1"
                     /translation="MRKRTTSRAKETIKEATTKATEAVKETVEKTTVKASEVIEEAKD
                     AAADIAKAPVVEEAKETVKKAVKEVAKNIDIKKFAETTIELPGFAVTMSSIEEAIKKD
                     IKDKGIEGKEISVYVNVEQKAAYYTVDGQGSDDYRIDLNTL"
     gene            complement(991..2373)
                     /locus_tag="ACZPD3_08305"
     CDS             complement(991..2373)
                     /locus_tag="ACZPD3_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1416023.1"
                     /translation="MINTLSAGKDNFYRQMLKLTIPIIIQNLLSAAVNSADVIMLNYV
                     GQSAISAVSLAANYSNILFMVYYGLGTGASLLCAQYFGKNNMQAIHAIEGIALRFSII
                     ISGIVALIAFTAPQLMMKIFTSDQELISIGSSYLRIMGITYLCWGITEIYLAILRSIG
                     RVTISMALNMLAFILNIILNATFIFGLFGAPKLGATGVAIATAASRLIELVACVIVSF
                     LSKDVKLNLTFMFIRSKVLFGDFVRLSLPALGNDVSWSVAFSMYSVILGHLGTDAVAA
                     NSLVTVVRNIGSVFCFAIASAGTILLGNVMGKGDLEKSKVYASRMLKMTIIAGAIGGV
                     IVFAITPLVLRFASLSDGAMHYLKYMLLINTYYIMGSAVNTALIAGVFRAGGDTKFGL
                     ICDTIDMWVYAVPLGFFAAFVLKLPVLWVYFLLCTDEFVKWPWVFHHYRQGKWAQNIT
                     REDLFEQKAS"
BASE COUNT          891 a          568 c          573 g          635 t
ORIGIN      
        1 aaatcctggg ctatacaaat ggcgagatca gcaggctgta cattctgtct acttcaatgg
       61 tagtagtgct ctgtctgctt gtcagtcttc caatagaaac ggcggttatg aaggttctgt
      121 tcagggagat gatgctgtca agcatttccg gctggattac gctctggatc gacccgatga
      181 tctatgtgca gatgtttgca gcaggaatca ttacatacgg aatcgtggcc ttgctggaat
      241 ttcggagagt gaagaaggtt cctatggatg aagccttaaa aaatgtcgag taggacaaaa
      301 tttgataaaa atttgacatt atctggaatt tgtgatacgg ttacacaaga aactgttaga
      361 gcgacacgct ctagaccaat cagttggaat aggagattat tatgagaaaa agaacaactt
      421 caagagcaaa ggaaactatt aaagaagcaa caacaaaggc tacagaagca gtcaaagaga
      481 cagttgagaa aacaactgta aaagcttcag aagtaattga agaagcaaaa gacgcagcag
      541 ctgatattgc gaaggctccg gttgtagaag aagcaaaaga aactgtaaag aaagcagtaa
      601 aagaagtggc taaaaatatc gacatcaaaa aattcgcaga aacaactatc gaattacctg
      661 gttttgcagt tacaatgagc tccatcgagg aagcaatcaa aaaagacatc aaagacaaag
      721 gcatcgaagg aaaagaaatc agcgtttatg taaatgttga gcagaaagcc gcttactaca
      781 cagtagacgg acagggctct gacgattaca gaatcgacct gaatacatta taatctgtaa
      841 gcatttttta taaaccgaaa atggatgtct gcagggatcc ggaaagaaaa tctgcagaca
      901 taaaaacagc aggattgttc atagaatgaa cagacctgct gttttgctta tttatgatag
      961 aaccacactg cggcggatat ggatgatatc tcaggaagct ttctgctcaa acagatcctc
     1021 ccttgtaata ttctgtgccc atttcccctg gcggtaatga tggaataccc atggccattt
     1081 cacaaactca tctgtacaga gcaggaagta aacccacaat accggaagct tcaggacaaa
     1141 ggccgcaaaa aatcccagag gtaccgcata tacccacata tcaatggtat cgcagatcag
     1201 tccgaacttc gtatcaccgc ctgcgcggaa taccccggcg atcagggctg tgtttaccgc
     1261 agaacccata atataatagg tattgataag cagcatgtac ttcaaataat gcattgcccc
     1321 gtcacttaag gatgcaaatc tcaggaccag aggcgtgatg gcaaatacga tcacaccgcc
     1381 aatggcaccg gctataatgg tcatcttcag cattctggaa gcatacacct tcgatttctc
     1441 cagatcccct tttcccatta catttccaag aagaatcgtt cccgcactgg caattgcaaa
     1501 gcagaacaca gaaccgatat tgcggacaac cgtaaccaga gagttggctg caacggcatc
     1561 tgttccgaga tgtcccagga tcacagagta catggagaaa gcaacactcc aggaaacgtc
     1621 atttccaaga gcgggaagtg acagccggac aaagtcgcca aacagcacct tactgcggat
     1681 aaacataaag gtcaggttca atttaacatc tttgctcagg aaagaaacga tcacgcatgc
     1741 taccagttcg atcagtctgg aagcagcagt tgcaatggca acacctgtag cccccagctt
     1801 cggtgcgccg aaaagcccga agataaaagt ggcattcagg ataatattca atataaaagc
     1861 cagcatattc aatgccatac tgatcgttac acgtccgata ctgcgcaaaa tcgccagata
     1921 aatctctgta attccccagc agagatatgt aattcccata atcctaagat aagaagaacc
     1981 gatagagatc agttcctgat cagaagtaaa aatcttcatc ataagctgag gagcagtaaa
     2041 tgcgatcagt gctacgattc ccgaaattat gatggaaaag cggagggcaa ttccctcgat
     2101 cgcatgaata gcctgcatat tattttttcc gaaatactgc gcacacagaa gagatgcgcc
     2161 tgtaccaagt ccataatata ccataaacag gatattggag taattggcag ccagagacac
     2221 cgcagaaatc gcagactgtc ccacataatt aagcataatt acatctgcgg agttaaccgc
     2281 tgcactgagc agattctgga taatgatcgg aatcgtcagt ttaagcatct gccggtaaaa
     2341 attatcctta ccggccgata atgtgtttat catatttacc cccgtaataa gtatttgccc
     2401 gatcaggcag ttttattagt ctgctttcag cataatcctt ccggcagaaa aacgctatcg
     2461 gtattttgac tgatttctac gttattttga cttttgagtc cttaaacaaa tcatctttgc
     2521 ttataatatc ttgcacaaaa gaagtcggac tgatataatt cgtatgtata ggtaattgcg
     2581 aaactcgcgg tgctcgttcg taaccttgaa ccaaaacaag gaagaattcg cgaagagcac
     2641 gacttgtgga atgaaaaatg gataatc
//
LOCUS       DBMDXG010000308         2658 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8415_length_2658_cov_1.235432, whole genome shotgun sequence.
ACCESSION   DBMDXG010000308 DBMDXG010000000
VERSION     DBMDXG010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8415_length_2658_cov_1.235432"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="ACZPD3_08310"
     CDS             complement(<1..504)
                     /locus_tag="ACZPD3_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIR1416024.1"
                     /translation="MQKIQFYYFTGMEAEQYSFYRVPKVLFTEPCFKTLSCEAKVLYG
                     LMLDRMSLSIKNRWLDSEDRVYIIFTVEEIAELMNCGTQKAVKLVKELDSSNGIGLIE
                     KKRLGLGKPNVIYVKNFMIREVPDQKSTGSCADSQSEQEDHKKIADLQSKQKTYKKCA
                     DLQQEAEN"
     gene            complement(434..1390)
                     /locus_tag="ACZPD3_08315"
     CDS             complement(434..1390)
                     /locus_tag="ACZPD3_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1416025.1"
                     /translation="MRRPKKDIQLTSYDELLGLEETSEKSMNQVVEIELEKLYPFKNH
                     PFHVNDDEKMAETVESIKTYGVLTPALVRPRPDGGYEMISGHRRKRGCELCGKTTLPA
                     LVRNYTDDEAVIIMVDSNIQRENLLPSEKAHAYKMKYDAMKHQGSKGEKYTADMVGEA
                     AGDSGRTVQRYIRLAALSDALLEYVDNNKIPMIVGEKLSYLKPEEQEWLLEVIANSSI
                     FPTKQQAEQLKECSANGKLNQSYIYAVLLKKESSKINVTIPAKKIGNYFPETYSKEQI
                     EEVIFMLLDKWKENKEGVADAEDTVLLFYRDGSGTIQLLSCP"
     gene            complement(1383..2159)
                     /locus_tag="ACZPD3_08320"
     CDS             complement(1383..2159)
                     /locus_tag="ACZPD3_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1416026.1"
                     /translation="MCKVIAIANQKGGVAKTTTTINLGAGLVKSGKKVVLVDADPQGH
                     LTMGLGFPKNLKVTLKSMMENIIMGLEFDPKEAILHHEEGVDLIPSNKLLAGMDMSLF
                     TVEDREKVLKEYLELLKDEYDYILIDCMPSLGMLTINALSAANSVLIPVQPQYYAADG
                     LMELLKVVKGIHQRFNPDLQIEGILFTMDNCRYNNAKRNKQAIKTTYGSDIKIFEQTI
                     PRTESLAETASEGVSIFAYDEKSKGADSYLELVQEVLKHA"
     gene            complement(2175..2339)
                     /locus_tag="ACZPD3_08325"
     CDS             complement(2175..2339)
                     /locus_tag="ACZPD3_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416027.1"
                     /translation="MLKIRLQGTLKDIQWFRRILEKHKELDVLEVSDAYANKGTSKYF
                     RVYVEVEEKE"
     gene            complement(2326..>2658)
                     /locus_tag="ACZPD3_08330"
     CDS             complement(2326..>2658)
                     /locus_tag="ACZPD3_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6075 family protein"
                     /protein_id="HIR1416028.1"
                     /translation="YCLGIDRDTRENVNKIYNFKTGCVKTESLQEGWQTSGSLRIVRM
                     AFNLYCNGTPSVGDYEAEEDQLKECQYYTVEDLFCCGYARYFWEAIKIRYPEYCFYKD
                     WEDMYAEN"
BASE COUNT          657 a          656 c          411 g          934 t
ORIGIN      
        1 gttttctgcc tcttgttgca agtccgcaca tttcttatat gttttctgtt tcgattgcaa
       61 atctgcaatt tttttatgat cttcctgctc agattgcgaa tctgcacagc tacctgtcga
      121 cttttgatct ggaacctctc tgatcataaa atttttcaca taaataacat tcggttttcc
      181 cagtcccaga cgtttcttct ctatcaatcc aatcccatta ctgctgtcca gctcttttac
      241 cagtttcact gccttttgag ttccacagtt catcaactct gcaatttctt ccacagtgaa
      301 aataatgtat actctgtcct ctgaatccag ccatctgttc ttaatgctca ggctcatacg
      361 gtccagcatc agaccgtata atacctttgc ttcacaagaa agagttttaa agcatggttc
      421 agtaaacagc actttaggga cacgataaaa gctgtattgt tccgcttcca tccctgtaaa
      481 ataataaaac tgtatcttct gcatctgcta ctccttcttt gttttctttc catttatcca
      541 aaagcatgaa aatgacttct tctatctgtt ctttagaata cgtctccgga aaataattgc
      601 cgattttctt tgccgggatc gtcacattga tcttactgct ttcctttttt aataaaacag
      661 catagatata actctggttt aattttccat ttgcactgca ttcttttaac tgttctgcct
      721 gttgctttgt cggaaagata ctgctgttgg caatcacttc cagaagccac tcctgttcct
      781 ctggcttcag ataagatagc ttttctccta caatcatggg aattttatta ttgtccacat
      841 attccagcag agcatcggat aatgccgcca gacggatata tctctgtaca gttctcccac
      901 tgtctcctgc ggcttcccca accatatctg ctgtatattt ctcaccttta cttccctgat
      961 gcttcattgc atcatatttc atcttatagg catgagcttt ctcacttggc agtagatttt
     1021 cacgctgaat gttggaatcg accataataa tgaccgcttc atcatccgta taatttcgga
     1081 caagagctgg caatgtagtc tttccacaca gttcacagcc tctttttctt ctatgcccgg
     1141 aaatcatctc atatccgcca tctggtcgag gtcttaccag tgcaggagtc aaaacaccgt
     1201 atgttttaat gctctctacg gtttctgcca ttttttcatc atcgtttaca tggaacggat
     1261 gatttttaaa gggatagagc ttttccagtt ctatttctac aacctggttc atactctttt
     1321 ccgatgtttc ctctaatccc agtaattcat cataagaggt aagctgaata tcttttttcg
     1381 gtctacgcat gtttcaacac ctcctgaacc agttccagat agctgtctgc ccccttgctt
     1441 ttttcatcat aggcaaagat acttacaccc tctgatgctg tttctgcaag gctttctgtt
     1501 ctcggaatcg tctgctcaaa aatcttaatg tcacttccgt atgtagtttt gattgcctgt
     1561 ttatttctct ttgcattatt gtaacggcaa ttatccattg tgaataaaat gccttctatc
     1621 tgtaaatctg gattgaacct ctgatgaatt ccctttacca ctttgagtaa ttccatgagt
     1681 ccatctgccg cataatattg tggctgcacc ggaatcagaa cactgttcgc tgcactgaga
     1741 gcattgatgg tcagcattcc cagagatggc atacagtcaa tcagaatata atcatactcg
     1801 tccttcagca gttccagata ctctttcagc accttttctc tgtcctctac tgtaaataag
     1861 gacatatcca ttccggcgag caatttattg gacggaatca ggtcaacccc ttcttcatgg
     1921 tgaagaattg cttcttttgg atcaaattct agtcccatga tgatattttc catcatgctc
     1981 tttaatgtta ccttgagatt tttagggaat cccaatccca ttgtcaagtg cccctgtgga
     2041 tcagcatcta ccaggactac ttttttccct gacttcacca gtccggctcc cagattgatt
     2101 gttgtcgttg ttttggctac tccgcctttc tgatttgcaa tagcaattac tttacacata
     2161 tactttcctc cgatttactc tttttcttct acttcaacat aaacacgaaa atatttgctt
     2221 gttcctttgt tcgcataagc atccgatact tccagaacat ccaattcttt atgcttttcc
     2281 aaaatacgac ggaaccattg aatatccttt aaagtaccct gtaatctaat tttcagcata
     2341 catatcctcc cagtctttgt aaaaacaata ctctggataa cggattttaa tggcttccca
     2401 aaaataccgg gcatatccgc agcaaaacag atcttctacg gtataatact gacactcttt
     2461 taactgatct tcctctgctt cataatctcc gacacttggt gtcccattgc agtaaagatt
     2521 aaatgccatg cggacgatcc gtaaacttcc actggtctgc caaccttcct gcagggattc
     2581 cgtttttaca catccagttt taaaattata gattttattc acattttctc ttgtgtctct
     2641 gtcgataccc aagcaata
//
LOCUS       DBMDXG010000309         2653 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8427_length_2653_cov_1.815831, whole genome shotgun sequence.
ACCESSION   DBMDXG010000309 DBMDXG010000000
VERSION     DBMDXG010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8427_length_2653_cov_1.815831"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..831)
                     /locus_tag="ACZPD3_08335"
     CDS             complement(181..831)
                     /locus_tag="ACZPD3_08335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR1416029.1"
                     /translation="MMTKTIIFDYDGTIHHTLGIYEPAFRETYQWLTEQKVTEEREIG
                     SVEIAGWLGLNSKEMWNTFLPELDQSYKEQASRMVGDLMVRQIRKHKAVWYPGAEEML
                     TALKNRGYHLVILSNCKASYREAHWKEFGMERWFDRFYDCESYRFRPKTEIVQEIIRE
                     YSGPYLVVGDRRQDLECARSCKSPFIGCLYGYGEKGELNGADYYAEHIEDILQMVI"
     gene            complement(847..1320)
                     /gene="ribH"
                     /locus_tag="ACZPD3_08340"
     CDS             complement(847..1320)
                     /gene="ribH"
                     /locus_tag="ACZPD3_08340"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR1416030.1"
                     /translation="MRQINLVEGKVVAPEGMKVGIVAARFNEIIVNKLLGGAVDGLVR
                     HGVEEENITAAWVPGAFEIPITAQKMAQSGKYDAIICVGAVIRGDTSHYDLVCNESAK
                     GIAQVELATGIPVLFGVITTENIEQAIARAGSKAGNKGYDCALSAIEMVNLMKQL"
     gene            complement(1350..2567)
                     /locus_tag="ACZPD3_08345"
     CDS             complement(1350..2567)
                     /locus_tag="ACZPD3_08345"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR1416031.1"
                     /translation="MSQNYQYNTIEEALRDLKEGKIVLVTDDPDRENEGDLICAAEFA
                     TRENINFMATYAKGLICTPMSAEIAVRLNFPPMVAENTDNHSTAFTVAVDHADTTTGI
                     SAEERSYTIMKCVDDQSKPEDFRRPGHVFPLISRKGGVLVRNGHTEATTDLMRLAGLK
                     ECGVCCEVMKEDGTMMRTSQLWEMAKEHNLTFITIRDLQDYIRIHEKHVKEEAVANLP
                     TQYGDFKMYGYINDITGEHHLALVKGDLGDGEDVLCRVHSECLTGDAFGSLRCDCGLQ
                     LQTAMRQVEAEGRGIILYMRQEGRGIGLINKIKAYALQEQGYDTVEANVKLGFAPDLR
                     EYWVGAQILSDLGVKSLRLLTNNPDKVYGLGEFGLKINERVPLEIPAQKYDRKYMKTK
                     QEKMGHIFKEINL"
BASE COUNT          627 a          723 c          423 g          880 t
ORIGIN      
        1 acttgagaac tttactgtac agatggggtt aggaaagtgg attacctcta tcactatgac
       61 tatcctaaaa tcatccccgc ctcagaattt gcaacaaatt gttgcaaatt ttctatatcc
      121 ttttatttta cagaagtact tatttttaac ttttttcctt cagattttat attgcaaaaa
      181 tcatatcacc atctgcagta tatcctctat atgttctgca taataatcag ccccattcag
      241 ttcccctttc tcaccatatc catacaggca tcctataaaa gggcttttgc aggacctggc
      301 acattccaga tcctgtctcc tgtctccgac taccagataa gggccactat attcccggat
      361 gatttcctgc acaatttctg tctttggacg gaatctgtat gattcacagt cataaaaacg
      421 atcaaaccat cgttccatcc caaattcctt ccagtgtgcc tcacgatatg aagctttaca
      481 gttacttaag atcacaaggt gatatccccg gttcttcaaa gctgttagca tctcctccgc
      541 tcccggatac cacactgcct tatgtttcct gatctgtctt accatcagat ctcctaccat
      601 cctgcttgcc tgctctttat agctctgatc cagttccggc aaaaatgtat tccacatctc
      661 cttactgtta agtcccagcc agccggcaat ttccaccgag cctatctccc gttcttcggt
      721 aaccttctgt tctgtcagcc actgataggt ttcacgaaat gccggttcat agattccaag
      781 ggtatgatgg atcgttccat cataatcgaa tatgatcgtt tttgtcatca tttattttcc
      841 tgtcttttag agctgcttca tcagattgac catctcaatc gcagataatg cacaatcata
      901 gcctttattt cccgctttgc ttcctgcacg cgcaatagcc tgctcgatat tttcagtcgt
      961 gatcactccg aataaaaccg ggattcctgt tgcaagttca acctgtgcga ttcccttggc
     1021 agattcatta cacaccagat cataatgtga agtatctcca cggatgacag ctccaacgca
     1081 gataattgca tcatattttc ctgactgtgc catcttctgt gctgtaatcg gaatctcaaa
     1141 tgctcccggt acccatgctg cagtaatgtt ctcttcttct actccatgtc ttaccagtcc
     1201 gtcgactgcc cctcccaata atttattcac aataatttca ttgaatctcg ctgccacgat
     1261 accaaccttc attccctccg gtgctactac ttttccttct actaaattga tctgtctcat
     1321 tttttcttcc tcctgatatt tgctgttttt tataaattta tttctttaaa aatatgaccc
     1381 atcttttcct gcttcgtctt catatacttc cggtcatact tttgtgccgg aatctccaga
     1441 ggtactctct cattgatctt cagtccgaat tcgccaagtc catagacttt atccggatta
     1501 ttggtcagta atcttaatga tttcacacca aggtctgaca gtatctgtgc cccgacccag
     1561 tactctctga gatccggtgc aaaccctaac ttcacattag cctccacagt atcgtatccc
     1621 tgctcctgaa gcgcatatgc tttgatctta ttgatcagtc cgattcctcg gccttcttgt
     1681 ctcatataca gaatgattcc tcggccttct gcttctacct ggcgcatcgc tgtctgcagc
     1741 tgcaggccac agtcacatct gagcgatccg aatgcatccc ctgtcagaca ctccgaatgt
     1801 acccggcaaa gcacatcctc accatctccc agatcacctt ttaccagtgc cagatgatgc
     1861 tctcctgtaa tatcattgat ataaccatac attttaaaat caccgtactg cgttgggagg
     1921 tttgcaacgg cttcctcctt tacatgcttt tcatgtattc ttatataatc ctgcagatca
     1981 cggatcgtaa taaatgtaag gttatgctct tttgccattt cccatagctg tgatgtacgc
     2041 atcatagttc catcttcttt cataacttca cagcacacgc cgcattcttt cagtccggca
     2101 agtctcataa gatctgttgt tgcctctgta tggccatttc gtaccaaaac cccgcctttt
     2161 ctggaaataa gcggaaacac atgtcccggt cttctgaaat cctccggttt tgactgatca
     2221 tccacacact tcatgatagt ataggagcgc tcttccgcag agatacctgt ggttgtatct
     2281 gcgtgatcta ctgccacggt aaatgctgta ctgtgattgt ccgtattctc cgcaaccatc
     2341 ggcggaaagt tcaatctcac cgcaatttct gcactcatcg gtgtacagat aagacccttt
     2401 gcataggtcg ccatgaaatt aatattttct ctggtcgcaa actctgccgc acatatcaga
     2461 tctccctcat tttctctgtc cggatcatct gtgaccagaa ctatttttcc ttccttaaga
     2521 tccctaagtg cttcttcaat tgtgttgtac tgataatttt gtgacatagt gtatatcctc
     2581 ctgttctaaa acccgaattc ttcaagaaac tccatcgtaa ttccggattc ttttttctct
     2641 tcttcgtttt ttc
//
LOCUS       DBMDXG010000310         2642 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8472_length_2642_cov_1.398268, whole genome shotgun sequence.
ACCESSION   DBMDXG010000310 DBMDXG010000000
VERSION     DBMDXG010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8472_length_2642_cov_1.398268"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="ACZPD3_08350"
     CDS             complement(<1..178)
                     /locus_tag="ACZPD3_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR1416032.1"
                     /translation="MVHFVGAGPGAPDLITVRGKQYLEEADVVIYAGSLVNPKLLEYT
                     KDICTIYNSAKMTLE"
     gene            complement(204..1370)
                     /gene="cbiD"
                     /locus_tag="ACZPD3_08355"
     CDS             complement(204..1370)
                     /gene="cbiD"
                     /locus_tag="ACZPD3_08355"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR1416033.1"
                     /translation="MKNGLEDYYVIRGNKKMRFGYTTGSCAAAACKGAAEILLGGVMH
                     KAVTLMTPKGILLTLELKDIRIEGNQVTCAIQKDAGDDPDTTNGILVYATVQKTKEPG
                     IILDGGVGVGRVTKAGLSQKIGEAAINPVPKAMILREATEIAEKYDYEGGLKIIISVP
                     EGVEIAKKTFNPRLGIVGGISILGTSGIVEPMSEAALVQSINVEMKQHFSQGEEYLLV
                     TPGNYGADYLREHMDLPYEKNIKCSNYVGETIDMAIDMGVKGILFIAHIGKFVKVAAG
                     IMNTHSHSADARMEVLASNAIRAGASLECAKEILNASTTDEAIDILEKYQILQKTMKE
                     ILDRIQFYLNHRSYEQILLGAVIFNNTYGYLGQTADAEKLIKLINAQNDETQLQ"
     gene            complement(1692..>2165)
                     /locus_tag="ACZPD3_08360"
     CDS             complement(1692..>2165)
                     /locus_tag="ACZPD3_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1416034.1"
                     /translation="GIGLMTVVGQCLGAGRKDEAVYYIKKLSVLAEIIVVASCLLVFA
                     LTIPVTKLGGMESESARMCFHMISWITVVKPIVWTLAFIPAYGLRAAGDVKFSMLTSC
                     ITMWTFRFCLCVYLIRFRGFGPMAVWIGMFTDWTVRGIVFSLRFHSRKWLKHKVV"
     assembly_gap    2166..2175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2176..>2642)
                     /locus_tag="ACZPD3_08365"
     CDS             complement(<2176..>2642)
                     /locus_tag="ACZPD3_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1416035.1"
                     /translation="FYTALSFPFIALYDAGASIFRSQGNTRGPMTVSVISNVINIGGN
                     AALIWGFNMGVAGAAIATLASRVFCAVVVLWQLRMDRQPIVVRNYRQIRPDGKMIRRV
                     LALGIPSGIENSMFQLGKLAIQSSVSTLGTVAIAAQAMTNILENLNGIAAIGVG"
BASE COUNT          693 a          672 c          480 g          787 t
ORIGIN      
        1 cctccagtgt catttttgca ctgttgtaga tggtgcagat gtcctttgtg tattccagaa
       61 gctttggatt taccagtgat cctgcataga tcaccacatc tgcttcctcc agatactgtt
      121 ttcctcttac tgtgataaga tccggtgctc ccggacctgc tcctacgaaa tgtaccataa
      181 tttatcattc tccttttata aatttattgt aactgtgttt catcattctg tgcattgatc
      241 agctttatca gcttctctgc atctgcagtc tgccccagat acccataagt attattaaaa
      301 atcactgccc ccagaaggat ctgctcatag gatctgtgat ttaaataaaa ctggatcctg
      361 tccaagatct ctttcatggt cttctgaagt atctgatatt tttccagaat gtctattgct
      421 tcatctgtag tagatgcatt caggatttcc tttgcacact ccaacgatgc tcctgcccgg
      481 atcgcattgg atgccagcac ttccatcctg gcatctgcac tgtgggaatg ggtattcatg
      541 attcctgctg caacttttac aaactttccg atatgagcaa tgaaaaggat tcccttaact
      601 cccatatcaa ttgccatgtc aatggtctca cctacataat tgctgcattt aatatttttt
      661 tcataaggga gatccatatg ctcccgcaga taatctgccc cgtaatttcc cggtgttacc
      721 agaagatatt cttctccctg ggaaaaatgc tgcttcatct ctacattgat actctgtaca
      781 agagcagctt cgctcatagg ttctacaatc ccggaagttc ccagaattga aattcctccc
      841 acaattccca gacggggatt aaaggttttc tttgcaattt ccacaccttc aggaacggag
      901 atgatgattt tcagaccacc ctcataatca tatttctcag cgatttctgt ggcttcacgc
      961 aaaatcatgg cctttggcac aggattaata gctgcttccc cgattttctg ggaaagtccg
     1021 gcttttgtta cccttcccac accaacacct ccgtcgagga tgattcccgg ttcttttgtt
     1081 ttctgcaccg tggcataaac cagtattccg tttgtagtat ccggatcatc tcctgcatcc
     1141 ttctgaattg cacaggtcac ctgatttcct tcaatccgga tgtctttcag ttccagtgta
     1201 agcaggattc ccttaggagt catcaaggtg acagccttat gcatgacacc acccagcagg
     1261 atctctgcag cacccttaca tgcagctgct gcgcaggagc ctgttgtata gccaaacctc
     1321 atttttttat tgccacgtat cacgtagtaa tcttccagtc cgtttttcat gatatctcct
     1381 ttgctgcatt gttgtcacat cctgtctgca gattactata atgtcattta tataacatct
     1441 acataaaaag gagggatttc tgtgtgcaac aaaatccctc ctgaaagctc ttttcccgaa
     1501 atatcctctg gttgccgcag atcatatttc ttatatacaa tgaaaagcag acagtatctg
     1561 gctgttacag taacggactt gcgcaggaat ttcacctgac ttcctgtaat gcttttccag
     1621 aattatttcc gagaatcatt atataatttt gagcagtaaa agtcaacgca tttcatggct
     1681 ataccacctt gttatactac cttatgcttc agccatttcc tactgtggaa gcgaagagaa
     1741 aatacgattc cccgcactgt ccagtcagta aacattccaa tccatacagc cattgggccg
     1801 aagcctctga aacggatcag atatacacaa agacaaaaac ggaatgtcca catagtgata
     1861 caggatgtaa gcattgaaaa ttttacatct ccggctgctc tgaggccata agcaggaata
     1921 aacgccaggg tccatacaat aggtttgaca accgttatcc aggatatcat atggaaacac
     1981 attcttgcac tttccgattc cataccaccc agctttgtga ccggaatagt taaggcaaac
     2041 accaaaagac agcttgcaac aacaatgatt tctgcaagaa cacttaattt cttaatatag
     2101 taaactgcct catcctttct gcccgctccc agacactgtc ctactacagt catcagaccg
     2161 attccnnnnn nnnnncctac accgatagct gcaataccgt taaggttctc aaggatattc
     2221 gtcattgcct gagctgcaat ggctaccgtc cccagtgtag atacggaaga ctgtattgcc
     2281 agctttccaa gctgaaacat gctgttttct atgccggacg ggattcccag agccagaact
     2341 cttctgatca tttttccatc cggacggatc tgtctgtaat ttctgactac aataggctgt
     2401 ctgtccatac gaagctgcca gagtaccact acagcacaga agactctgga tgcaagagtt
     2461 gcgatggcag ctcctgccac tcccatattg aatccccaga tcagcgccgc atttccgccg
     2521 atattaatca catttgaaat aaccgatact gtcattggcc ctctggtatt tccctgggat
     2581 cggaatatgg atgctcccgc atcataaagt gctataaacg gaaaagacaa cgccgtatag
     2641 aa
//
LOCUS       DBMDXG010000311         2641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8475_length_2641_cov_1.535039, whole genome shotgun sequence.
ACCESSION   DBMDXG010000311 DBMDXG010000000
VERSION     DBMDXG010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8475_length_2641_cov_1.535039"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /gene="fliB"
                     /locus_tag="ACZPD3_08370"
     CDS             complement(<1..515)
                     /gene="fliB"
                     /locus_tag="ACZPD3_08370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HIR1416036.1"
                     /translation="MQYTFPNYYKEFSCIAGACPDTCCAGWQIVIDDQTLKKYQHFKG
                     PFRNRLHNDIDWKEHVFRQYNRRCAFLNEENLCDIYTEAGPEMFCRTCRNYPRHIEEF
                     EGLREISLSLSCPEAARILLSQKEKVHFITKEKKTKEEVYDDFDYFLFTALMDTRDML
                     IDIIQDRAVPM"
     gene            complement(520..1359)
                     /locus_tag="ACZPD3_08375"
     CDS             complement(520..1359)
                     /locus_tag="ACZPD3_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1416037.1"
                     /translation="MSSQSFRFSPEHPTAQNITLLNTSSSRYEGDWPSIPHSHAFTEL
                     FYVRDGQGDFLIEDQIFPISKDDLVIINPHINHTEISKGSPPLSYFTVGVDGLCFSFN
                     GHKEYRIFNCREKKTDLLFYFNSLFQELDRQSEGYETICSHMLDILILQLRRITDSPF
                     ELITAQHPSKECAHIKRYLDSNYGENITLDHLSELSHLNKYYLSHEFTKYYGISPINY
                     LNRKRIEVCKELLENTDYGISDIAHLTGFSSQSYLSQSFRKSCGMTAGTYRKLKKKRK
                     DPI"
     gene            1752..>2641
                     /locus_tag="ACZPD3_08380"
     CDS             1752..>2641
                     /locus_tag="ACZPD3_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase N-terminal domain-containing protein"
                     /protein_id="HIR1416038.1"
                     /translation="MGKGRVTIPTDLDVVPQTLEMIEEWGADAIRDCDGTEFPQKLKD
                     TGAKIYATYYTTRKDNAWAKAHPEEIQQMYIMTSFHTAVADTLEIHLMDHLYPNMLAV
                     NTRDDIRRWWEVMDRTTGEPVLTDQWSYEEETGNVVILSAKRFHEYTVSFLAYIMWDP
                     VHMYNAVVNDWKDVEPQITFDVRQPKTRAHSLERLRRFLDTHEYVDVVRFTTFFHQFT
                     LIFDELAREKYVDWFGYSASVSPYILEQFEKEVGYPFRPEYIIDQGYMNNTYRIPSKE
                     FKDFQAFQRREVAKLAKEMV"
BASE COUNT          830 a          476 c          581 g          754 t
ORIGIN      
        1 tgcatgggta cagctctgtc ctggatgata tctataagca tatcacgggt gtccataagt
       61 gcagtgaaca ggaaataatc aaagtcatca tagacttctt cttttgtctt tttttctttt
      121 gtgataaagt gtaccttttc tttttgggaa agtaaaatcc ttgctgcctc cggacaggag
      181 agcgacaggg aaatctcacg cagaccttca aattcttcaa tatgtctagg ataatttctg
      241 caggttctgc agaacatttc cggtcctgct tctgtgtaga tatcacacag gttctcttcg
      301 ttgagaaaag cacagcgtct gttatactgg cggaaaacat gttctttcca gtcaatatca
      361 ttgtgcaggc gattgcggaa tggaccttta aagtgctggt acttcttcag tgtctgatcg
      421 tcaatcacga tctgccatcc tgcgcagcag gtatccggac aggctcctgc aatgcaggaa
      481 aattctttat agtagtttgg aaatgtgtat tgcatgatat taaataggat cctttctttt
      541 tttcttaagt ttacggtaag ttcctgcagt catgccacag gatttgcgaa agctctggga
      601 gagataactc tgagaggaga atcctgtgag atgcgcaata tctgaaatcc cgtaatcagt
      661 attttccaga agctccttgc acacttcgat gcgttttcgg ttcagataat tgataggaga
      721 aatcccataa tattttgtaa attcatgact cagataatac ttattaagat gactgagttc
      781 tgagaggtga tccagagtaa tattttcccc gtaattggag tcaagataac gcttaatatg
      841 agcgcactct ttacttggat gctgggcggt gatcagttca aatggtgaat ccgtaatgcg
      901 ccgaagctgc agaatcagaa tatccagcat atgactgcag atggtttcat atccttcaga
      961 ctgtctgtcc agctcctgaa acagagaatt aaagtaaaat aacaaatctg ttttcttttc
     1021 acgacagtta aaaatgcgat actctttatg accgttaaag gaaaaacata gtccgtccac
     1081 acctactgta aaataactca gaggaggact ccctttggag atttcagtgt gattgatatg
     1141 tggattaatg atgaccaaat cgtcctttga gattgggaaa atctgatcct cgatcagaaa
     1201 atctccctga ccgtcacgta catagaacaa ttctgtaaag gcatgagagt gaggaatact
     1261 gggccagtca ccctcgtagc gggaagaaga ggtatttaaa agcgtaatat tctgagctgt
     1321 tgggtgttcg gggctgaaac gaaatgattg tgagctcatg gcagattctc ctttcttctg
     1381 tttacggcag atgagtatat ttgtagcaat tttgttggcg gattctgaat gatagtattt
     1441 tatatcgcaa aagatataac tgagattctt caggataatt agaataagtg cataaaaaat
     1501 gataaaaata aaatcaacat cagttattaa ggtaaaaaat agaaggcttt tcagatagct
     1561 gataacatat tttctataat accataattt ttttgtaaga gcaagatttc taaaaaacaa
     1621 agcaaaaagt gaattgaaaa aaattttgaa agaaagtatt ttataaacag atagactgaa
     1681 aataatgtga ttacagaagc gaacggacag taattatccg cttgtcagca aaatgaagga
     1741 ggtaacagaa aatgggaaaa ggtcgtgtaa ccataccaac tgacctggat gtagttccac
     1801 agacactgga aatgatagaa gagtggggcg ctgatgctat acgtgactgt gacggaacag
     1861 aatttccgca gaaattaaaa gacacaggtg ccaaaattta tgcaacctac tatacaactc
     1921 gtaaggacaa tgcgtgggcg aaagcacatc cggaagaaat ccagcagatg tatattatga
     1981 catctttcca cacagcggtt gcagatacac tggagattca tctgatggat cacctctatc
     2041 cgaatatgct tgcagtcaac acaagagatg atatccgcag atggtgggaa gtaatggatc
     2101 gtaccacagg agagccggta ctgacagatc agtggtcata tgaagaagag acaggaaatg
     2161 tagtgatctt atctgcgaaa cgcttccatg aatatactgt aagtttcctg gcatatatta
     2221 tgtgggatcc tgtacatatg tacaatgcag tagtaaatga ctggaaagac gtagaaccgc
     2281 agattacatt tgatgtgcgt cagccgaaaa caagagcaca ctctctggaa cgtctgcgca
     2341 gatttctgga tactcatgag tatgtggatg ttgtgcgctt cactacattt ttccatcagt
     2401 ttactcttat atttgatgaa cttgcaagag agaaatatgt agactggttt ggttattccg
     2461 cttcagtcag cccatatatt ctggagcagt ttgagaaaga agtgggctat cctttcagac
     2521 cggaatatat cattgatcag ggatacatga acaataccta tcggattccc tcaaaagaat
     2581 tcaaagattt ccaggcattt cagagacgcg aagttgcgaa gcttgccaaa gagatggtag
     2641 a
//
LOCUS       DBMDXG010000312         2638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8487_length_2638_cov_2.418210, whole genome shotgun sequence.
ACCESSION   DBMDXG010000312 DBMDXG010000000
VERSION     DBMDXG010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8487_length_2638_cov_2.418210"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="ACZPD3_08385"
     CDS             <1..343
                     /locus_tag="ACZPD3_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1416039.1"
                     /translation="RLLASMSGKENIDIEKALKVVGLECAGRKKVGKYSMGMRQRLGI
                     AQAIMENQSILILDEPMNGLDNKGVDEIRKLILDLKKEGRSIILASHNREDIQILCDQ
                     VYEMDNGKIIG"
     gene            complement(454..1380)
                     /locus_tag="ACZPD3_08390"
     CDS             complement(454..1380)
                     /locus_tag="ACZPD3_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR1416040.1"
                     /translation="MPLKNSLFLLLAAIIWGIAFVAQSVGMEYVGGFTFSAVRSLLGS
                     VVLIPLILISGRKNSADTAATKANSFVGSSDGTVSSIRKRKDLIIGGISCGICLCLAS
                     NFQQFGIKYTTVGKAGFITACYIVIVPIIGLFLGKKCSKFIWAAVAMALIGLYLLCIT
                     DGFSIGKGDLLVLVCAFLFSIHILVIDHFSPKADGVKLSCIQFLTCGILSGIPALLFE
                     HPEFSAICRAWMPILYAGIMSCGVAYTLQIIGQKNMNPTVASLILSLESCISVLAGWV
                     LLGQQLSAKEILGCVIMFAAIILAQLPQKQAE"
     gene            complement(1471..1587)
                     /locus_tag="ACZPD3_08395"
                     /pseudo
     CDS             complement(1471..1587)
                     /locus_tag="ACZPD3_08395"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            1546..1686
                     /locus_tag="ACZPD3_08400"
                     /pseudo
     CDS             1546..1686
                     /locus_tag="ACZPD3_08400"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            complement(1831..>2638)
                     /locus_tag="ACZPD3_08405"
     CDS             complement(1831..>2638)
                     /locus_tag="ACZPD3_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR1416041.1"
                     /translation="LLITAILLVKNVKGNILWGILITWGLGIICQFIGLYKPDAAAGW
                     YSLLPDFSNGISIPSMAPTFMKMDFSIVFTLDFVVIMFAFLFVDMFDTLGTLIGVASK
                     ADMLDKEGKLPNIKGALLSDAVGTTVGAMCGTSTVTTFVESASGVAEGGRTGLTSLVA
                     AILFGLSLLLSPIFLAIPSFATAPALIIVGFLMLTSVTKIDFNDMTEAIPAFIAIIAM
                     PFLYSISEGISMGVISYVIINVVTGKAKEKKISVLMYVLAVLFILKYIFI"
BASE COUNT          823 a          489 c          649 g          677 t
ORIGIN      
        1 tagattactt gcatcaatgt cgggaaaaga aaatatagac attgaaaaag cattaaaagt
       61 tgtaggatta gaatgcgctg gtagaaaaaa agttggaaag tattctatgg ggatgagaca
      121 gagacttgga attgcacagg caattatgga aaatcagagt attttgattt tggatgagcc
      181 catgaatggt ttggataata aaggggtaga tgaaatacgt aagttgattt tggatttgaa
      241 aaaagaggga cggtcgatca ttctggcaag tcataacaga gaagatattc aaatcttgtg
      301 tgatcaagtg tacgaaatgg ataatggaaa gattataggg tgatattata gcaattccac
      361 aaaataatgc aatcaagaca gttcagaaga gcgcgaagtc ctgattgcat tattttatgg
      421 aattgctata gaaaatacat atgctaaggg gtattattct gcctgtttct gtggcagctg
      481 tgcgaggatg atcgcggcaa acatgatcac gcatccgagg atttcttttg cacttaactg
      541 ctgtccaagg agaacccatc cggcaaggac ggagatgcag gattccaggc tgaggatcag
      601 ggatgccaca gtcggattca tgttcttctg tcctatgatc tgcagggtgt aggctacacc
      661 gcaggacata atacctgcat agaggattgg catccatgcc cggcagattg cagaaaattc
      721 cggatgctca aacagcagtg caggaatacc ggaaaggatt ccgcaggtca ggaactggat
      781 gcaggaaagt tttactccgt cagctttcgg agaaaagtgg tcaatgacca gaatgtgtat
      841 ggagaataag aatgcgcata ccagtacaag caggtcacct tttccgatgg agaatccgtc
      901 tgtaatgcac agaaggtaaa gaccgatcag tgccattgca acagcagccc agatgaattt
      961 gctgcatttt ttgccaagaa acagaccgat gatcggtaca attacgatat aacaggcggt
     1021 aataaatccg gcttttccga cagtggtgta tttaatgccg aactgttgga agttgcttgc
     1081 cagacaaagg cagattccgc aggaaatacc gccgatgatc aggtcttttc gtttccggat
     1141 gctggataca gtgccgtcgg atgagccaac gaaactgtta gcttttgtag cagcagtatc
     1201 ggcggagttt ttgcgtccgg aaatgagaat aagcggaatc agtacaactg acccgagcag
     1261 ggaacggact gcactaaatg taaatccacc gacatactcc attcctacgc tctgagccac
     1321 aaaggcgatg ccccagataa ttgctgccag gagcagaaac agggaattct ttaaaggtat
     1381 ctgttgtttt tgcatttgta aaaaatcctt ccttataaat agtgatgtgt aaatgaaatg
     1441 tataaaatgt ggataatgca aaatatttat tcatacagtg tggacagaag cagtagagtg
     1501 tgtctgaaaa atgctttctg caagatgtgc gtcacaattt gtgggatatt ttgcccggat
     1561 gagggcgccg tagcgggcta cggcaactga attcgggtga aatatcctgc aaagtggggc
     1621 gtgcagattg cgggaatgat ttttcagaca tgctctagaa actgcaaata tcccgctgat
     1681 ttataaaatg aataaacact ttcataatta tacaacacag accaggaaag tgaaagaaaa
     1741 ttatggtaac aaaaaagaag cccctgcagg aggcttcttt ttcgcaaatg tgtaaatttg
     1801 cgtttttagt tttgttgttg actttccgga tcagataaag atatatttca gaatgaacag
     1861 cacagcaagt acatacatca gtacactgat cttcttttct tttgctttac ctgtaacaac
     1921 gttgatgatc acataggaga taacacccat ggagatacct tcagaaatgc tgtacaggaa
     1981 tggcattgcg atgatcgcga taaatgccgg gattgcttca gtcatgtcgt tgaagtcaat
     2041 cttggttact gatgtgagca tcaggaatcc aacgatgatc agagccggtg ctgtggcaaa
     2101 ggatgggatt gccaggaaga tcggggataa cagcagtgat aatccgaaaa ggatggctgc
     2161 taccagggaa gtaagtcctg tacgtccgcc ttcagcaaca ccggaagcac tctctacgaa
     2221 agtagtaact gtggaagtac cacacatagc acctactgta gttcctactg cgtcagaaag
     2281 aagagcgcct ttgatgtttg gaagtttgcc ttctttgtca agcatatcag ctttggaagc
     2341 aacaccgatc agggttccca gagtatcaaa catatctaca aacaggaatg caaacataat
     2401 gactacgaaa tcaagtgtga agacaatgga gaagtccatt ttcataaatg taggagccat
     2461 gcttggaatg gagattccgt ttgagaaatc cggcaggaga ctgtaccagc ctgctgctgc
     2521 atccggtttg taaagtccga taaactgaca gatgattcca agtccccatg tgatcaggat
     2581 accccagagg atatttcctt ttacattctt tacgagaaga atggcggtga tcagaagt
//
LOCUS       DBMDXG010000313         2636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8498_length_2636_cov_1.375937, whole genome shotgun sequence.
ACCESSION   DBMDXG010000313 DBMDXG010000000
VERSION     DBMDXG010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8498_length_2636_cov_1.375937"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2449)
                     /locus_tag="ACZPD3_08410"
     CDS             complement(<1..2449)
                     /locus_tag="ACZPD3_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR1416042.1"
                     /translation="MAASKSKNTGHSGRNTKKKTAMEQEVGRFQTEIILFVILAACVI
                     LMASNFGAGGFVGDAISNFCFGLMGLMAYLFPIVFFLESAFILINKTNRLAYKKLAAA
                     FVMFLFLCGAAQLLTDGYMQSTTLLDYYALSADYKTGGGLIGGAICISVTSAFGTIGG
                     YVIIILAFVVCMIIITQRSLLDFVTRLVIKVCDFVKDRHMRYMQGQPERERYREERAR
                     ERPLIREQRREERVRRLEEEIGDDEFSDDDFLMDPSEAKKMKKGGFLEGTKLTGYSEQ
                     KPKKKGKLLKKAADTDKAAADTAEAAEDTENTGIKSSDDADNKPYIDAPMNFEIHRAD
                     TSPDTYEADNESQQQEDLPFEDAVTDRKKSVVSEPEEDLPPFQDEKAVRPPSKNPKSS
                     EKEIQSGIVNIQHEITRQEAAVKKEYKFPALNLLKKGSSKAQGDSDAYLRKTAKKLQE
                     VLHNFGVNVTVTNVSCGPTVTRYELQPEMGVKVSKIVGLADDIKLNLATPDIRIEAPI
                     PGKAAVGIEVPNKENSTVMLRDLLQSEEFQKAKSKLSFAVGKDIAGKTVVADIAKMPH
                     LLIAGATGSGKSVCINTLIISILYKANPDEVKLIMIDPKVVELSVYNGIPHLFIPVVT
                     DPKKAAGALNWAVQEMTNRYNTFAEYGVRNLDEYNKKAEQIKAAGAEEEPVKMPQIVI
                     IVDELADLMMVAPGEVEDAICRLAQLARAAGIHLIIATQRPSVNVITGLIKANMPSRI
                     AFSVSSGVDSRTILDMNGAEKLLGKGDMLFYPQGYQKPARLQGAFVSDDEVSAVVEFL
                     ADKNPGVQYNQQIEQQVN"
BASE COUNT          660 a          661 c          504 g          811 t
ORIGIN      
        1 aatttacctg ctgctcgatc tgttggttat actggacacc cggattttta tctgcaagaa
       61 attcaaccac agcacttacc tcatcatcag atacaaatgc accctgaagt cttgcaggct
      121 tctgatatcc ctgaggataa aagagcatat caccttttcc aagaagcttc tcggctccgt
      181 tcatatccag aattgtcctg gaatccacac cggaagatac agagaaagca attctggaag
      241 gcatattcgc cttgatcaga ccggtgataa catttacaga cggtctctgt gtggcaatga
      301 tcagatggat acctgccgca cgtgcaagct gggccaggcg acagatagca tcctccacct
      361 ctccgggcgc aaccatcata aggtctgcaa gctcatccac gataatcacg atctgcggca
      421 ttttcacagg ttcttcttca gctcctgccg ccttgatctg ctctgctttt ttattatatt
      481 catccagatt acggacaccg tactccgcaa atgtattgta acggtttgtc atctcctgta
      541 ctgcccagtt taatgcgcct gctgctttct tcggatctgt cacaaccgga ataaacaaat
      601 gtggaattcc attatacaca ctgagctcta ctactttagg atcgatcatg ataagcttta
      661 cttcatccgg atttgccttg tacaggatac tgatgatcag tgtattgata catactgatt
      721 tacctgaacc ggttgcacct gcgatgagaa gatgtggcat tttggcaata tccgcaacta
      781 cagtcttacc tgcaatatcc tttcctactg caaaagaaag tttggatttt gccttctgaa
      841 attcttctga ctggagcaga tctctgagca taaccgtact gttctcttta ttgggaacct
      901 caatgcccac tgctgctttg ccggggatcg gtgcttcaat acggatatca ggagtggcaa
      961 gattcagttt gatgtcatct gcaagaccta caattttgct gaccttcact cccatctcgg
     1021 gctgaagttc ataacgggta actgttggtc cgcagctcac attagtgaca gtcacgttca
     1081 cgccgaaatt gtgaagtact tcctggagtt tttttgcagt cttacgcaga taggcatcag
     1141 aatccccctg agctttgctg cttcctttct tcagcagatt taaggctgga aatttatatt
     1201 ctttttttac ggctgcttcc tgtcgggtga tctcatgctg aatgtttaca attccgctct
     1261 gtatctcttt ttcagaagat ttggggtttt tcgaaggcgg ccgcactgcc ttctcatcct
     1321 gaaaaggggg cagatcttcc tccggttctg aaaccacaga tttcttccgg tcagtgacag
     1381 catcttcaaa tggaaggtct tcctgctgtt gtgattcatt atctgcctcg tatgtatccg
     1441 gggatgtatc cgctctgtga atctcaaaat tcatcggagc atcgatgtat ggtttattat
     1501 ccgcatcatc agaggattta atccctgtgt tctcggtatc ctccgcagct tcggctgtat
     1561 ctgctgctgc tttatctgta tctgctgctt ttttcagaag tttccccttt ttctttggct
     1621 tctgttcaga atagcctgtc agcttggttc cttccagaaa gccgcctttt ttcatttttt
     1681 ttgcttccga aggatccatg agaaagtcat catctgaaaa ttcatcatcg ccaatttctt
     1741 cctccagtct gcggactctt tcttcacgcc gctgttctct tataagcgga cgttctctgg
     1801 cacgttcttc tctatatcgc tcacgttcag gctgcccctg catatagcgc atatgtctgt
     1861 cttttacaaa atcacacacc tttataacca gccgcgttac aaaatcgagc agagagcgct
     1921 gtgtgataat gatcatacat acaacaaatg ccagtataat gatcacatat ccgccgatag
     1981 ttccaaaagc agatgtgaca gaaatacaga tcgctcctcc gatcagacca ccgcctgttt
     2041 tataatctgc gcttaaagca taataatcca gcaaagttgt actctgcata tatccgtctg
     2101 tgagaagctg cgctgcccca cacagaaaca gaaacatcac aaacgccgct gcaagtttct
     2161 tatatgccag ccggtttgtt ttattgatca gaataaatgc tgattcaagg aaaaatacga
     2221 ttggaaacag atatgccatc agtcccatca gtccgaaaca gaaattactg atcgcatctc
     2281 ccacaaaacc acctgctcca aaattgcttg ccatcaggat cacacatgcg gccaaaatca
     2341 caaataatat gatttctgtc tgaaatctgc cgacttcctg ttccattgct gtcttctttt
     2401 ttgtatttct tcccgaatgc ccggtattct tactctttga tgctgccatt ctaaggctcc
     2461 cctccttgat cttctcgtaa atatcttccc tttacagggc aaaatttaat gccagtgtaa
     2521 taataccgac tataaatgaa taatatgcaa aatagcgtaa ttttacattc tgagtcatgg
     2581 ctattgtatt tcggataaca agacatcccg caaaaccggc gatgatcatt gcaaat
//
LOCUS       DBMDXG010000314         2634 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8506_length_2634_cov_1.055270, whole genome shotgun sequence.
ACCESSION   DBMDXG010000314 DBMDXG010000000
VERSION     DBMDXG010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8506_length_2634_cov_1.055270"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="ACZPD3_08415"
     CDS             complement(<1..1512)
                     /locus_tag="ACZPD3_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HIR1416043.1"
                     /translation="MQSSNQLQDMLHSINRKSYPAYKSLKGAYQFNKYVLSIDHVQGD
                     PFASPSHISVKIFHREAGFPAEYYKDKLTRITLADYLTRRFEQQVNRYTFRAKGSGKS
                     GLISVTRCGQEVLERTACEITDQGIIARFFVGFPANGRTINAGELEKIFFELLPVCVE
                     KAFVYRNLSGKDLENTIFLAEDQAYIREELKKRFLVAFVNDGAILPRESGISSKPMKG
                     SVTFSSPESLRVTMALPHKGKITGMGIPKGITLIVGGGYHGKSTLLNALELGVYNHIQ
                     GDGREYVITDSTAQKLRSEDGRFVKDVDISLFINDLPNKKDTTCFSTEDASGSTSQAA
                     GIVEGIEAGSKVFLLDEDTSATNFMVRDAFMQHVISREKEPITPFLERARDLYEKEGI
                     STILVAGSSGAFFHIADTVIQMDNYIPVDITEKVRELCRDYPLNENTAAGFKAPQSHR
                     IMSKNTPLNGSKKDYYGHFKVQDKPERLKVKVHGRDGFSIGKQEVDLRYVEQLI"
     gene            complement(1766..2194)
                     /gene="tnpA"
                     /locus_tag="ACZPD3_08420"
     CDS             complement(1766..2194)
                     /gene="tnpA"
                     /locus_tag="ACZPD3_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HIR1416044.1"
                     /translation="MDNKSLSHTRWKCQYHIVFIPKYRKKVLYGKVREDVREIISTLC
                     KYKSVEIIAGAVCIDHVHLSVAIPPKFSVSDFMGYLKGKSTLMLYDRHPELQSKWDKA
                     FWARGYYVETIGNITDAAVQKYINEQAEESRREDSSSTAL"
     gene            complement(2327..>2634)
                     /locus_tag="ACZPD3_08425"
     CDS             complement(2327..>2634)
                     /locus_tag="ACZPD3_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR1416045.1"
                     /translation="YGSILPALRAVYCGQTVFGTEIVSRIPDLLQGEKKFDYGKYDIN
                     EREFEMIELIANGYSNKEIAAELFLSEGTVRNYLSMILEKLQLRDRTQVAVFYYQHK"
BASE COUNT          668 a          681 c          468 g          817 t
ORIGIN      
        1 gatcagctgt tccacatatc gcagatccac ttcctgtttt ccaatggaaa aaccatctct
       61 gccatgtacc tttactttca gcctttccgg cttatcctgc accttaaaat gaccgtaata
      121 atccttcttg gaaccgttca acggcgtatt ttttgacatg atgcgatggc tttgtggtgc
      181 tttaaatcca gctgccgtat tctcatttaa aggataatcc ctgcaaagtt cccggacttt
      241 ttctgtgata tctactggta tataattgtc catctggatc acggtatctg caatatggaa
      301 aaaagccccg gagcttccgg caaccagaat tgtggaaatc ccctcctttt catacagatc
      361 ccgggcgcgt tccaggaatg gggtgatcgg ctctttttcc cggctgatca catgctgcat
      421 aaaggcgtcg cgcaccataa aattagtagc agatgtatct tcatccaaca gaaatacctt
      481 gcttccagcc tctattccct ccacgattcc tgctgcctgg gaagtacttc cgctggcatc
      541 ttccgtagaa aaacaggtgg tatctttttt attgggaagg tcatttataa acagggaaat
      601 atccacatct ttcacaaatc tgccatcctc agaacgcagc ttctgggctg tggaatccgt
      661 gatcacatat tctcttccat ctccctgaat gtggttatac acgcccagtt ccaatgcatt
      721 cagaagggta gatttgccgt gataaccacc accaacgatc aacgtgattc ccttaggaat
      781 tcccattccg gttatttttc ccttatgggg aagcgccata gtcacccgaa gactttccgg
      841 cgaagaaaat gtcacgctcc ccttcatcgg cttagatgaa ataccgctct ctcttggcag
      901 aatcgcgccg tcatttacaa aagcaaccag gaaacgcttc tttaattctt ccctgatata
      961 cgcctgatct tctgccagga aaatagtatt ttccagatct tttccactta aatttctgta
     1021 tacaaatgct ttttccacac aaaccgggag aagttcaaag aatattttct ccagttctcc
     1081 cgcatttata gtacgtccat ttgccggaaa tcctacaaaa aaccgggcga taatcccctg
     1141 atctgtaatc tcacaggccg ttcgctccag cacctcctga ccgcatctgg tcacagaaat
     1201 aagtccgctt tttccagagc cttttgcccg gaaagtatag cggttcacct gctgttcaaa
     1261 ccgtctggta agatagtctg ccagagtaat tctggtcagt ttatctttat aatattccgc
     1321 tggaaatcca gcttctctgt gaaaaatttt cacactgata tgggacggtg acgcaaatgg
     1381 atcgccctgt acatgatcaa ttgacaaaac atatttgtta aactgatatg cccctttcag
     1441 ggatttatag gcaggatagc tcttccgatt gatggaatga agcatgtcct gcagctgatt
     1501 tgatgactgc ataagtacct ccattgtcta taaaaatgaa tatcgccaat cactgatatt
     1561 actgtctaat tcttcaccgc actcagcagt aaagccacta cggcattcaa acatacatga
     1621 agcacgagcc tattcccacc ccttctcata tatgtttcct agttagcaac caccagttca
     1681 actggtggtt tatccttggc cctccaaagg gccattgtta ctgctcgccc caaagggcgg
     1741 tatcgcaagc acttttactg gcccgctata aagcggtact actggaatct tctcttctcg
     1801 attcctctgc ttgctcattt atatatttct gaactgctgc atctgttata ttgccgattg
     1861 tttcaacata atatcctcta gcccaaaagg ctttatccca tttactttgc agttctggat
     1921 gtctgtcgta aagcatcaat gtgcttttcc ctttcagata tcccataaag tctgatacac
     1981 taaatttcgg tggaattgct acgcttaaat gcacatgatc gatacatacg gctcccgcaa
     2041 ttatttctac acttttatat ttgcatagag ttgatattat ttctctcaca tcttctcgta
     2101 cctttccata caatactttc ttcctatatt taggaatgaa tacgatatga tactggcatt
     2161 tccagcgagt atgtgataga cttttattgt ccatttggac cgcctccttt gattttgagt
     2221 ttggcttgcg acaccattct catcatatca caggaggctt tattttgtta tcctcaccgc
     2281 ttccgcctac tccattctcc ccagcatagc tgggggatta ggcttttcat ttatgctggt
     2341 aataaaaaac tgccacctgt gtacggtcac gcaactgcag tttttccaga atcatgctca
     2401 gataattccg cacggttcct tcactcagga aaagctctgc cgcaatctcc ttattgctgt
     2461 acccatttgc gatcagctct atcatctcaa attctctctc gttaatgtca tattttccat
     2521 aatcaaactt cttttctccc tgcaaaaggt ctggaatcct ggaaactatt tctgtcccaa
     2581 atactgtctg cccacagtaa accgcccgaa gtgcaggcag aatactgcca taat
//
LOCUS       DBMDXG010000315         2623 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8541_length_2623_cov_1.663362, whole genome shotgun sequence.
ACCESSION   DBMDXG010000315 DBMDXG010000000
VERSION     DBMDXG010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8541_length_2623_cov_1.663362"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            244..1050
                     /locus_tag="ACZPD3_08430"
     CDS             244..1050
                     /locus_tag="ACZPD3_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR1416046.1"
                     /translation="MKKLKVFSISAILIAICCSFLFSASVSAASKKRNKFDHKPSGNI
                     YYYDENGHTVKGLVTIRGKKYYFNETGIQQNGWQKIKGDYYFFQIRNGCYASMVTSRR
                     VNGIYLTKSGEARYNSEEKRKLNLMVTANQVMRRVTKRNMSKPEKLWRCYLKAVSYGY
                     GGTGNDYDFRYYYSNWDVSYAEDMFYRGHGDCFAFASAFAYLANAVGFEAKVISSGGH
                     GWAEIKGEVCDPNWAKGTGHIERYYRMSYDLSGVDGRPYYRGNRAYVITI"
     gene            1334..>2623
                     /locus_tag="ACZPD3_08435"
     CDS             1334..>2623
                     /locus_tag="ACZPD3_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1416047.1"
                     /translation="MQNNKNFLGTEPVGKLLLKLSIPTVIAQLINMLYNIVDRIYIGH
                     IPGEGSLALTGVGVCMPIIMIVSAFAALVSSGGAPRASIYMGKQDNKSAENILGNCFI
                     LQIIISVILTAILLIWGRDLLLAFGASENTISYATDYMHIYAFGTLFVQLTLGMNAFI
                     TAQGFTTFSMVSVLIGAVCNIVLDPVFIFVFHMGVRGAALATVISQAISTLWVVSFLC
                     GKKTLLHLRKKHLHLEAKVVLPCIALGLAAFIMQSSESIVTVCFNSSLLRYGGDIAVG
                     AMTILTSVMQFAMLPLQGIAQGAQPISSYNYGAKNADRVKKTFRLLLITCLSYSVLLW
                     AAVQLVPRVFVSIFTADTDLIGFTAPMLKIYLGGLFLFGIQIACQMTFTSLGKAVNSI
                     IVAVVRKFVLLIPLIYIMPHMISDSTTGVYMAEPIADI"
BASE COUNT          707 a          563 c          547 g          806 t
ORIGIN      
        1 tccagattgc ctgcggcaat ggaattttgg cttgtatgtc tcgggatttt ggcatattca
       61 tgccaaaaca cctcgcggga tagtggtgtg tgaacagtaa cgctttttct ccttaattga
      121 atattgcgca cagttaaatt gtcttatata ataaaatata tcttatgatt agagcagata
      181 atttcttcat tgtacattat ttatcagaaa taaatatctg ctcataaaga acggaggtgc
      241 tctatgaaaa aattaaaagt attttctatc tcagcaattc ttattgctat ctgttgctct
      301 tttttatttt cagcttcagt atccgcagca tcaaaaaaac gtaacaaatt tgaccacaaa
      361 cccagcggaa acatttatta ctatgatgaa aacggacata ctgtaaaggg acttgtcacg
      421 atccgcggaa agaaatatta ttttaatgaa acgggaatcc agcagaacgg ctggcagaag
      481 atcaagggag attattattt cttccagatc cgcaacggct gttatgcctc catggtaact
      541 tcccggcgtg taaatggtat ttatcttacc aaaagtggag aagcacgcta taacagtgaa
      601 gaaaaaagaa aactgaacct tatggtcaca gctaaccagg tcatgcgtcg tgtgaccaaa
      661 agaaatatgt ccaaaccaga gaaattatgg agatgttatc tgaaggcagt cagctacgga
      721 tacggtggca ccggcaatga ctacgatttc cgttactatt actcaaactg ggatgtcagc
      781 tatgcggagg atatgttcta ccgggggcat ggcgactgtt tcgcttttgc atctgcattt
      841 gcttacctgg ctaacgcagt cggatttgaa gccaaggtta tctcatcagg cggtcatgga
      901 tgggctgaga tcaaaggaga agtctgcgat ccgaactggg caaaaggcac aggacatatc
      961 gaaagatatt accgtatgag ttatgacctc agcggtgtag acggacgtcc ctattataga
     1021 ggaaacaggg cgtatgtaat tacaatctga tctcatccgg acattttctg acatatcagg
     1081 cagataaaac tatacgcttc gctgcctgat ttatagtaat cctctaaaat aacgcatctt
     1141 aatccaaaaa gttccgggta tctgtatctg ccagatgtcc cggaactgtt actgttcact
     1201 ccgttcgtta ctgttcactc cgttcacagt aacgttgccc aaattctcta tcctgagttg
     1261 actttccttt ctttatatcg tatcattaaa tattattcat tcaaaaatat ttttaagaaa
     1321 gaggttatca tccatgcaga ataacaaaaa ttttctgggc acagaacctg ttggaaagct
     1381 tcttttaaag ctatccattc caacagtgat cgcccagctc atcaacatgc tttataacat
     1441 tgtagaccgt atttacatcg gccacatccc gggggaaggc agcctggcac ttaccggtgt
     1501 gggagtctgt atgcccatca tcatgatcgt ctccgcattt gcggcactgg tcagttccgg
     1561 cggcgcacca agggcttcta tttacatggg aaaacaggat aataaatctg ctgagaatat
     1621 attaggcaac tgttttattc ttcagattat tatctctgtt atcctcactg ccattttact
     1681 gatctggggc agagatttat tactggcttt tggcgccagt gaaaacacca tcagttatgc
     1741 aacagattat atgcatattt acgctttcgg taccttgttc gtacaattaa cccttggtat
     1801 gaatgcattc attactgcac agggatttac cacattttcc atggtttccg tcctgatcgg
     1861 tgctgtctgc aatattgttc tggatcctgt gtttatcttt gtttttcata tgggggtacg
     1921 tggtgctgca ctggcaactg ttatctcaca ggcgatttcc acactctggg tagtttcatt
     1981 cctctgcggc aaaaagacac tgcttcacct caggaaaaag catctccacc tggaggcaaa
     2041 ggtcgttctt ccctgtatcg cgctgggact tgctgctttt atcatgcagt caagcgaaag
     2101 cattgtgact gtctgcttca attcatccct gcttcggtac ggcggtgata ttgcagtagg
     2161 agccatgacc atcctgacca gtgttatgca gtttgccatg cttcctcttc agggaattgc
     2221 gcagggagca cagccaattt ccagttataa ttacggtgca aaaaatgcag acagagtaaa
     2281 gaaaacattc cgtttgcttc tgatcacctg tctgtcatat tctgtacttc tctgggctgc
     2341 tgtacagctt gttcccagag tatttgtaag tatttttact gctgatacag atcttattgg
     2401 atttactgca ccaatgctga agatctatct gggtggtctg ttcctcttcg gtatccagat
     2461 tgcctgccag atgaccttta cctcccttgg aaaggctgta aactctatta ttgttgctgt
     2521 tgtccgtaag tttgtactgc tgatcccgct gatctatatt atgcctcata tgatatctga
     2581 ttcaacaaca ggcgtatata tggctgaacc cattgcagat att
//
LOCUS       DBMDXG010000316         2622 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8543_length_2622_cov_2.545022, whole genome shotgun sequence.
ACCESSION   DBMDXG010000316 DBMDXG010000000
VERSION     DBMDXG010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8543_length_2622_cov_2.545022"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="ACZPD3_08440"
     CDS             <1..601
                     /locus_tag="ACZPD3_08440"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR1416048.1"
                     /translation="EEFEEKPMVSSYNTISTGIYVIRRRQLIELIERAALEGRHDFVK
                     DILIRYKNLKRIYGYKIDTYWSNISTAEAYYKTNMDFLKPEIRNYFFKQEPTIKTKID
                     DLPPAKYNPGAQVKNSLVASGCIINGTVENSVLFKDVFVGNNCVIKNSVILNNVYLGD
                     NTHIENCIVESRDTIRANSYYCGDGEVKIVVEKNDRYIL"
     gene            651..938
                     /gene="spoVG"
                     /locus_tag="ACZPD3_08445"
     CDS             651..938
                     /gene="spoVG"
                     /locus_tag="ACZPD3_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIR1416049.1"
                     /translation="MNITDVRVRKVAKEGKMKAVVSITIDDEFVVHDIKVIEGEKGLF
                     IAMPSRKATDGEYRDIAHPINSATRDRIQTIILDKFQEVMDAEPEEAAEAE"
     gene            1134..2456
                     /locus_tag="ACZPD3_08450"
     CDS             1134..2456
                     /locus_tag="ACZPD3_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcsB-like coiled-coil domain-containing protein"
                     /protein_id="HIR1416050.1"
                     /translation="MKKKVFSLLLAGMLTFSMGTTAYATEDAIADMQAQKQQAEAGLA
                     QTQANIDSLQSKKQELENYLSDLNTQYEDLTNAISELSIQAGEKENELKQLHTELKKA
                     KKALNKQYDDMKLRIQYMYENGGTSALETLLSSKDLSEFLNNAESVAKISQYDRTMLE
                     KCENLQNTIKDQETTAEEEKAAIDQLLEERAAKQQEVQNLAASTSDNISSYVSQISAS
                     QEEAAALTAEINNADNSIAQLVQQAEEEKAAREAAQAQAEAEAAAAQEQDAEESSDDY
                     EEDTDSYEEDSSDSQDSSETEGSEDTSEDSGDSDDEDTYEENDADFYEGDSDSNESED
                     TSDSQASSDSSSGQGKYLGNFTLTAYCNCAQCCGTAGNLTASGTVPTAGRTVAMAGVP
                     FGTQLLINGNVYTVEDLGTPYGHVDIYCDSHSEALSFGLQSAEVYQLN"
BASE COUNT          896 a          455 c          639 g          632 t
ORIGIN      
        1 tgaggaattt gaagagaagc caatggtttc ttcttataat acaatttcaa caggtatcta
       61 tgttatccga agaagacagc tgatcgagct gatcgagaga gctgcgctgg aaggcagaca
      121 tgattttgta aaggatattc tgatccgcta caagaatctt aagagaatct atggatataa
      181 gattgacaca tactggagca atatctccac agcagaagca tattataaga caaatatgga
      241 tttcctgaaa ccggagatca gaaactattt cttcaaacag gaaccgacaa ttaagacaaa
      301 gattgatgat ctgcctcctg caaaatacaa tccgggagca caggtgaaga acagcctggt
      361 tgccagcgga tgtattatca atggtacggt ggagaattcc gttcttttta aagacgtatt
      421 tgtaggaaat aactgcgtga tcaagaactc tgtgatttta aacaatgtat atctgggaga
      481 taatactcat attgagaact gcattgttga aagccgggat actattcgtg caaattcgta
      541 ttactgcggc gacggtgaag tgaagatcgt cgtggagaaa aatgacagat atatactgta
      601 aataaaacag ccggatagat tcggtaaatc gaaaggagta aatttgtatc atgaatatta
      661 ctgacgtgcg tgtgaggaag gtagcaaaag aaggaaagat gaaagcggtt gtttctataa
      721 caatcgatga tgaatttgta gtacatgata tcaaggtgat tgaaggagaa aaagggttat
      781 ttatcgctat gccaagccgt aaggcaacag acggagaata tcgtgatatt gcacatccga
      841 tcaattctgc aacacgtgac aggatccaga cgatcattct ggacaagttc caggaagtta
      901 tggatgcgga accggaagaa gctgcagaag cggaataacc ggtgaataaa gaactgtaaa
      961 caaataagaa gaagcacagg cactgtctct gaaaaagggg cagtgcctgt tttggctgag
     1021 cattagtgaa gataaaatgt tacataatta ttacataaat taataaaaag tgttgatttt
     1081 tttacgaaat atgatatact gggtttcaga ttaacgagaa aggtgattaa tttatgaaga
     1141 aaaaagtttt ttcattactg ctggccggaa tgcttacttt ttccatggga acaactgcat
     1201 atgcaacaga agatgcgatc gctgatatgc aggctcagaa acagcaggca gaagccggac
     1261 ttgcccagac acaggcgaat attgacagcc ttcagagcaa gaagcaggag ctggagaact
     1321 atctgtctga tttgaataca cagtatgaag acctgactaa tgcaatttct gaactcagca
     1381 tacaggccgg agagaaagag aatgagctga aacagcttca cacagaatta aagaaagcca
     1441 agaaggcatt aaataaacaa tatgatgata tgaaactccg catccagtat atgtatgaga
     1501 atggcggtac atctgcatta gagactttgc tgtcatctaa ggatctttct gagttcctca
     1561 acaatgcaga gagtgttgcg aagatttccc agtatgacag gactatgctg gagaaatgcg
     1621 agaaccttca gaacactatc aaagatcagg aaacaactgc tgaggaagag aaagctgcta
     1681 ttgatcagct tcttgaagag agagcggcca aacagcagga ggttcagaat cttgcagcaa
     1741 gcacaagtga taacatctct tcctatgtaa gccagatcag cgcaagccag gaagaagcag
     1801 cagcactgac tgcagaaatc aacaatgcag ataacagcat tgctcagctt gtgcagcagg
     1861 cagaagagga aaaagcagcc agagaggcag cgcaggcaca ggccgaagca gaggcggcag
     1921 cggcacagga acaggatgca gaagagagtt ctgatgatta tgaagaagat acagattcct
     1981 atgaggaaga ctcttcggac agtcaggatt cttcggagac tgaaggatct gaggatactt
     2041 ctgaagattc aggggattct gatgatgaag atacatacga agagaacgat gcagatttct
     2101 atgagggaga ctctgacagc aatgaatcag aagatacatc tgacagccag gcatcctcag
     2161 acagttcttc cggtcaggga aaataccttg gcaactttac actgactgca tattgcaact
     2221 gtgcacagtg ctgcggtaca gcaggtaacc tgacagccag cggaacagtt ccgacagcag
     2281 gacgtactgt ggctatggcg ggagtaccct ttggaacaca gcttctgatc aacggaaatg
     2341 tatacacagt agaggatctg ggaacacctt atggacatgt tgatatttat tgtgacagcc
     2401 attcagaagc attaagcttc ggacttcagt ctgctgaggt ttatcagttg aactgataac
     2461 aaaacaataa tgtgattaag aactctggta aaacagggtt ctttttttca gatttttgtc
     2521 cggattgcga taacataggt ggtttaactt gaggattcag atgcctgatg ataaaatgat
     2581 atatcagatt tatatacggt aaaatataat tggaatggag at
//
LOCUS       DBMDXG010000317         2621 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8549_length_2621_cov_2.057143, whole genome shotgun sequence.
ACCESSION   DBMDXG010000317 DBMDXG010000000
VERSION     DBMDXG010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8549_length_2621_cov_2.057143"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(123..1436)
                     /locus_tag="ACZPD3_08455"
     CDS             complement(123..1436)
                     /locus_tag="ACZPD3_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR1416051.1"
                     /translation="MSKKIERKDRHFKFETLQLHVGQESPDPVTDARAVPIYQTSSYV
                     FRNCDHAAARFGLADAGNIYGRLTNPTEDVFEKRIAALEGGSAALAVASGAAAITYTI
                     ENLAQQGDHIVAAKNIYGGTTNLLEHTLPAYGITTTFVDVFDLEEVENAIQDNTKAVY
                     IETLGNPNSDVVDIEAIAKIAHAHKIPLVVDNTFATPYLVRPIEYGADIVVHSATKFI
                     GGHGTTIGGVIVESGKFDWEASGKFASLTEPNPSYHGVSFTKACGPAAFVTKIRAILL
                     RDTGATISPVSSFIFLQGLETLSLRVERHVENALKVVQYLNNHPQVEKVHHPSVSTDP
                     VQQELYKKYFPNGGGSIFTFEIKGDAQKAKDFIDNLELFSLLANVADVKSLVIHPATT
                     THSQCTEEELLDQGIKPNTIRLSIGTENIDDIIQDLDEAFKAVAE"
     gene            complement(1706..>2621)
                     /gene="mnmA"
                     /locus_tag="ACZPD3_08460"
     CDS             complement(1706..>2621)
                     /gene="mnmA"
                     /locus_tag="ACZPD3_08460"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1416052.1"
                     /translation="DARNVCYRMGIRYYVFNFSERFKEDVMDRFVDAYEHGATPNPCI
                     DCNRYLKFDKMFQRMRELEYDYIVTGHYARVEYDEEKNRYLLKKAVDDTKDQSYVLYM
                     LTQEQLAHISLPLGGLRKTEVREIAEKHGFVNARKHDSQDICFVPDGDYAKFIEQYTG
                     RKSIPGDFVDTEGNILGKHKGIIHYTLGQRRGLGIPAASRLYVCDISPKTNQVVLGNN
                     EDLFHSELTATKVNLISCESLKEPMRLKAKIRYRHPEQEAVAWQTEDGVLHVRFDKPQ
                     RAITRGQAVVLYDGDIVVGGGVIENCIK"
BASE COUNT          711 a          592 c          497 g          821 t
ORIGIN      
        1 gtaaaccata caattgaaac attccaaaaa aaagaccatc tttcagatat gggggaaaat
       61 ccggaagatg gtctttttat tcatgtttta tttatacaat atataaggat gttatatctg
      121 tattattcag caactgcctt aaatgcttca tcaagatcct gaatgatatc gtcaatattc
      181 tctgtaccaa tggaaagacg aattgtattt ggtttgatac cctgatcgag aagttcttct
      241 tctgtgcact ggctgtgtgt tgttgtagcc ggatggataa caagagattt aacatccgct
      301 acattggcaa gcaatgagaa cagttccaga ttatcaatga agtcctttgc cttctgcgca
      361 tcacctttaa tctcaaatgt aaagatggaa ccgccgccat tcgggaagta tttcttataa
      421 agctcctgct gaaccggatc tgtggataca gacggatgat gaactttctc tacctgcgga
      481 tggttattta agtactgaac aactttcagt gcattctcta catggcgttc aacacgaagt
      541 gacagtgttt caagtccctg aaggaaaatg aatgaactta ccggagagat ggttgcacct
      601 gtatcacgaa gaagaattgc acgaatcttt gtaacaaatg ctgccggtcc gcatgccttt
      661 gtaaagctta cgccatgata gcttgggttc ggttctgtca gagatgcgaa tttacctgat
      721 gcttcccagt caaatttacc actctcaacg atcacacctc cgattgttgt accatgtcca
      781 ccgatgaatt tagttgcaga atgaacaacg atatctgcac catattcgat tggtcttaca
      841 aggtaaggtg ttgcaaatgt gttatccact acaagcggaa tcttatgtgc atgagcaatc
      901 tttgcgatag cttcaatatc aactacatca gagttcgggt ttccaagagt ctcgatgtaa
      961 acagcttttg tgttatcctg aattgcgttc tctacttctt caagatcaaa tacatcaacg
     1021 aatgttgttg tgattccata agctggaagt gtatgctcta gaaggtttgt tgttccaccg
     1081 taaatattct tggctgctac aatgtggtca ccctgctgtg caagattctc aattgtatat
     1141 gtgatcgctg ctgcaccgga tgctactgca agtgccgcag aaccgccttc aagtgctgcg
     1201 attcttttct cgaatacatc ttcagtcggg tttgtaagac gtccgtaaat gttacctgca
     1261 tctgcaagtc cgaaacgtgc agctgcatga tcgcagtttc tgaatacata agaagatgtc
     1321 tgatagattg gtactgctct tgcatctgta accggatccg gactctcctg tcctacgtga
     1381 agctgtaatg tttcaaattt gaaatgtctg tcttttcttt cgattttttt gctcatgtgt
     1441 atatcctcat ctttctctga tttataataa tctttttctc tgcaaaactg attccggctc
     1501 ttaagcccct tatctgcaag tcatttttgt ttctacgatt ttcatatctg tttgatatga
     1561 tttagcatag ttgtatatta ccatttaatt cctagtacgt caataggaaa ttttctatag
     1621 cgtttaatag ctaataacta taacgtttcc aaaaaacagt gcagataaga cagtccgaca
     1681 aaatgcgaaa gacaaggatg atctgtcatt taatacaatt ttcaatcacg ccgccgccta
     1741 caacgatatc accatcatat aacacaactg cctgccctct ggtgatagct ctctgtggtt
     1801 tatcaaaacg cacgtgcagt actccatctt ccgtctgcca tgctacggcc tcctgctccg
     1861 gatgacggta acggatcttt gctttcagtc tcattggctc cttcagagat tcacaggaaa
     1921 tcagattgac ctttgttgca gtcagttccg aatggaaaag atcctcatta tttcccagaa
     1981 ccacctgatt ggtctttgga gaaatatcac acacatacag tctggatgct gccggaatac
     2041 caagtcctct tctctggcca agtgtatagt ggatgattcc tttatgttta ccaagaatgt
     2101 ttccttctgt atctacaaaa tcgcccggga ttgatttacg tcctgtgtac tgttcaataa
     2161 actttgcata atcaccgtcc ggtacgaaac agatatcctg gctatcatgc tttctggcat
     2221 taacaaagcc atgcttctct gcgatctcac gcacttccgt tttgcgaagc ccgccaagag
     2281 gcagggaaat atgcgcaagc tgctcctgtg tcagcatata aagcacataa ctctgatcct
     2341 tcgtatcatc cacagctttc tttaataagt aacggttctt ctcctcatca tattccacac
     2401 gtgcataatg tccggttaca atataatcat actccagttc acgcattctc tgaaacatct
     2461 tgtcaaattt cagataacga ttgcagtcaa tgcagggatt tggcgtggct ccatgctcat
     2521 aggcatccac aaagcggtcc ataacatcct ctttaaacct ctcggaaaag ttaaaaacat
     2581 agtacctgat ccccattctg tagcagacat ttctggcatc c
//
LOCUS       DBMDXG010000318         2591 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8649_length_2591_cov_1.773092, whole genome shotgun sequence.
ACCESSION   DBMDXG010000318 DBMDXG010000000
VERSION     DBMDXG010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8649_length_2591_cov_1.773092"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(123..1436)
                     /locus_tag="ACZPD3_08465"
     CDS             complement(123..1436)
                     /locus_tag="ACZPD3_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR1416053.1"
                     /translation="MRKGKGIALLPIGVFLVLYLGLGILFEYVMEIPMGFYNVPIVVA
                     FLAAILVACLQNRSLDFDKKLEIMAQGVGDKNIITMLLIFLAAGSFVGVVGRSSAESV
                     AYCMLSLIPARFSVSVLFIVACFVSVAMGTSVGTITLLTPIAAAVSTASGFDLAFCVA
                     SVMGGAMFGDNLSFISDTTIAACNGQGCEMKDKFRENFWIALPAAVATLILILILSFQ
                     TEIQGRVIQPYHLTQVIPYVLVLIGGIVGINVFVVLLTGIVSGAFIMLIGGHTTPVEI
                     LKNMGSGVSGMFETCMVAILVAAMCALIREYGGFDALLGWIHKIFRGKKGGQLGMGLL
                     VGTMDIATANNTVAIVMANPIAKEMAEEYGITPRKTASILDTFSCVFQGVIPYGAQML
                     VAISAVNELGGEISAFQIMPKLFYPMLLLFSSLITIMRGSDRTGA"
     gene            complement(1527..2381)
                     /locus_tag="ACZPD3_08470"
     CDS             complement(1527..2381)
                     /locus_tag="ACZPD3_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR1416054.1"
                     /translation="MKKQLNYADIIKEALILTGAVAIIAAAVYFFLVPSHTSVSSISG
                     LGIVLSNFVPLSLSAITMILNVVLLIIGFITCGREFGVKTVYTSIVLPLFLGLFEKLF
                     PDFGSMTNSQELDVLCYILVVSVGLSILFNRNASSGGLDIVAKIMNKYLHMELGKAMS
                     LSGMCVALSAALVYDKKTVVLSILGTYFNGIVLDHFIFDHNIKRRVCVITQKEEELRK
                     FIIEDLHSGATIYEATGAYNMKKRNEIITIVDKTEYQKLMAYINHEDPKAFVTVYNVS
                     DMRYQPKL"
BASE COUNT          766 a          630 c          480 g          715 t
ORIGIN      
        1 acagccgatc cgctgctcta ctgccctgtc tgtgcttcct ttatactgct tccacaagga
       61 ggaaggtcaa gtattttttg aaagaaattg aaaaaaatat agcttccggc agggggaatc
      121 ttttaagctc ctgtacggtc tgagcctctc ataattgtga tcagagaact gaacagcaat
      181 aacatgggat aaaataattt cggcatgatc tgaaatgcag aaatctcacc acccagttca
      241 ttcactgctg agattgcaac cagcatctgt gcaccatatg gaatcactcc ctggaaaaca
      301 catgaaaaag tatccagtat ggaagctgtc tttctcggtg taatcccata ctcctctgcc
      361 atttcctttg cgatagggtt tgccattacg atcgccactg tattattggc agtggcaata
      421 tccatagttc ctaccagaag tcccattcca agctgtccgc ctttctttcc gcggaaaatt
      481 ttatggatcc agccaagaag tgcatcgaat ccaccatact cacgtatcag cgcacacatg
      541 gcagctacca gaatcgccac catacaggtc tcaaacattc cggatacacc ggatcccata
      601 tttttcagga tctctacagg tgtggtatgt cctccgatca gcataataaa tgctccggat
      661 acgattcctg tcagcaatac cacaaacaca ttgatcccaa caattcctcc gatcaatacc
      721 agcacatacg gtattacctg agtcagatga tagggctgga tcacacggcc ctggatttct
      781 gtctgaaatg acaggatcag gattaagatc agtgtggcaa cggctgccgg aagagcgatc
      841 cagaagtttt ccctgaattt gtctttcatc tcacatccct gaccattaca tgcagcaata
      901 gtcgtatctg aaataaaaga aagattgtcc ccaaacatgg ctcctcccat aacagaagcc
      961 acacaaaatg ccagatcaaa tcctgatgct gtggaaactg ctgctgcaat cggtgtcaga
     1021 agggtaatgg ttcccacaga ggttcccata gccacagaca caaaacaggc aactataaac
     1081 agcacagaca ctgaaaatct ggcaggtatc agactcagca tacaatatgc aacactctca
     1141 gcactgcttc ttcccacaac tcctacaaaa gatccggctg caaggaagat cagaagcatg
     1201 gtgatgatat ttttgtcccc aactccctgt gccatgatct ccagtttctt atcaaaatcc
     1261 agcgatctgt tctgcagaca ggcaaccagg attgcagcaa gaaatgctac tacaataggc
     1321 acattataaa atcccatggg gatttccatc acatactcaa acaaaatgcc cagtccaagg
     1381 tataatacaa gaaatacacc aatgggaagc agggcaattc cttttccttt tctcatcttt
     1441 catcctcttt ctaccataat ctctttatgg ttatataatc actctgctat tttagcatac
     1501 ccacagcttt cctgccataa tatttctcac agcttgggct gatagcgcat atccgataca
     1561 ttgtaaaccg tcacaaaagc ctttggatct tcatgattga tgtaagccat cagcttctga
     1621 tattctgttt tgtctactat cgtgatgatc tcattgcgtt tcttcatatt ataggcacct
     1681 gtcgcttcat agattgtcgc tccgctgtgc agatcttcga taatgaattt acgcagttct
     1741 tcctctttct gagtgatgac gcagacacga cgtttaatat tatggtcaaa gataaagtga
     1801 tccagaacta ttccattaaa gtatgttccc agaatactca gcaccactgt tttcttatca
     1861 tatacaagcg ctgcggaaag agccacacac ataccggaaa gagacattgc ttttcccagt
     1921 tccatgtgga gatatttgtt catgatcttt gccacaatat ccagtccgcc ggaggatgca
     1981 tttctgttaa aaagaatgct cagtcctacg ctgaccacca ggatatagca cagcacatcc
     2041 agctcctgac tgttggtcat agagccaaaa tccgggaata atttctcaaa taatccgaga
     2101 aataagggca aaacgatact ggtgtaaact gtttttacgc caaattcgcg tccgcaggtg
     2161 atgaagccaa tgatcagaag taccacattc aaaatcatgg tgattgcaga caatgataaa
     2221 ggtacaaagt ttgataacac gataccaaga ccggagatac tgcttacaga ggtatggctc
     2281 ggcaccagga agaaataaac tgctgccgca atgattgcta ccgcacctgt caggatcagc
     2341 gcttccttta tgatatctgc ataatttagt tgttttttca tagttagatt tcctgtcttt
     2401 ctttccatat tcttgttcta ttgtaacata tgggaagaga atgtccatca gcaattacaa
     2461 aaagaggctg ccttccatga caaccccttt gtaaatcctt ataacctata ggactgctat
     2521 aaaattcgct ttcatatttt agatcgtgtt tgaaaaacac tctaagattt tatcctaacg
     2581 gctctgtatg t
//
LOCUS       DBMDXG010000319         2585 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8670_length_2585_cov_1.655797, whole genome shotgun sequence.
ACCESSION   DBMDXG010000319 DBMDXG010000000
VERSION     DBMDXG010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8670_length_2585_cov_1.655797"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="ACZPD3_08475"
     CDS             <1..201
                     /locus_tag="ACZPD3_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HIR1416055.1"
                     /translation="ANGAATKKMYLTVSHTEDGGMNFSYHYQTAHLEEHDMELLYYYM
                     MRILFKGIAEPDMSIGEIMELV"
     gene            228..458
                     /locus_tag="ACZPD3_08480"
     CDS             228..458
                     /locus_tag="ACZPD3_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1416056.1"
                     /translation="MNQEMEFKNIVAQYSKVAPEEMNNEMRFREDLGFSSLDFMSFLG
                     ELEDTFDLELDESEVLKITTLGEALNLLEELQ"
     gene            564..2108
                     /locus_tag="ACZPD3_08485"
     CDS             564..2108
                     /locus_tag="ACZPD3_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1416057.1"
                     /translation="MLIRNILEESAHKFDEVKAVKWLNKKEIMERSYGELMENVVSTR
                     KGLLAEGFEGKHIALIGTSSVEWMESYLGIITGCTTAVPLDAALPCEDLIDLLNRSDS
                     VALFLSPKLKPYLDAFLANCPKLQKVWMLQEEVEDAPAKVYGIGELRNAGKSTSADSV
                     CPDAEDIATIIFTSGTTGKSKGVMLTQKNLASNVEAVKITAEPGTAMLSVLPIHHAFC
                     LVMDWLKGFSLGATLCINDSLLHMVRNMSIFKPDIMLMVPMMIETIYKRLTAADPSIS
                     KTVLAEKVFGGKLRIIFTGGAHLDPYYIDRFAEYGVEVLEGYGMSECSPVISNNTPEN
                     HKKGSIGKPLENAEIRFENGEILVKGSSVMKGYYQMPDETAETLKDGWLHTGDKGYMD
                     EDGYLFINGRVKNLIILSNGENVSPEEIENKLALNPLIGEVIVTGEDNGLTARIYPEQ
                     AVVEAKALDAEAIQAQLQAFLDEYNRNQPTYRRITGLVVRKNPFIRNTTKKIRRQDVL
                     IDEPLE"
     gene            complement(2118..>2585)
                     /locus_tag="ACZPD3_08490"
     CDS             complement(2118..>2585)
                     /locus_tag="ACZPD3_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1416058.1"
                     /translation="ITKTAGIARSTFYNHYQDIPSLISAVEDKTIHDVFSMMENFQPK
                     NDRDICSSYFLTLCRYTMENPFLSGLLSTPHGNDLFEKMLTMLHHYVTETTSNSRPDR
                     HTKEEVSYVITCAIGSTIGVLHKWSRDNFNLAPEVIADILSEVFLSGMLPLLS"
BASE COUNT          812 a          475 c          630 g          668 t
ORIGIN      
        1 gcaaacggtg cagccaccaa gaagatgtat ctgaccgtat cccatacaga agatggaggt
       61 atgaacttct cctatcatta ccagactgct catctggagg aacatgatat ggaactgctc
      121 tattattata tgatgcgtat tcttttcaaa ggaatcgcag agccggatat gagcatcggc
      181 gaaattatgg aactggttta actaaatcaa tatcaggagg aaatgcaatg aatcaggaaa
      241 tggaatttaa aaatatcgta gcacagtaca gcaaggtagc accggaagaa atgaataatg
      301 agatgagatt tcgcgaggat ctgggtttca gttctctgga ttttatgtcc tttttaggtg
      361 agcttgagga tacctttgat ctcgaacttg atgagagcga agtgttgaag atcacaactc
      421 ttggtgaagc cttaaatctg ctggaagaac ttcagtaatt tttttcgaag taaaacttat
      481 aaagatgtca gggtcaaaga tattttgtac ctgacaggat atccataact tgaacagttc
      541 agaaaataat gggggaacag agaatgctga tcagaaatat attagaagaa agtgcacata
      601 aatttgacga agtaaaagca gtaaaatggc tcaacaaaaa ggaaatcatg gaaagaagct
      661 atggcgaact gatggaaaat gtagtttcca ccagaaaagg tcttcttgca gaaggctttg
      721 agggcaaaca cattgcactg atcggaacca gttctgtaga gtggatggaa agttatctgg
      781 gaatcatcac cggatgtacc actgcagtac cgctggatgc agcacttccc tgtgaggatc
      841 tgatcgattt gcttaaccgt tccgattccg tagctctttt cttaagtccg aagcttaaac
      901 catatctgga tgcatttctt gcaaactgcc cgaaacttca gaaggtgtgg atgcttcagg
      961 aagaggtaga agatgcaccg gcaaaggtat atggtattgg tgaacttcga aatgcaggaa
     1021 agagcacatc agcagattct gtctgcccgg atgcagagga tattgcaacc atcattttca
     1081 cttccggaac aacaggaaag agcaagggtg ttatgctgac acagaaaaat ctggcttcca
     1141 atgttgaggc agtaaagatt acagcagaac cgggaactgc catgctcagt gtgcttccaa
     1201 tccatcatgc attctgtctg gttatggact ggctgaaggg attttcgctg ggtgcaactc
     1261 tttgtatcaa tgattccctg cttcatatgg taaggaatat gagtattttc aaaccggata
     1321 ttatgcttat ggttccaatg atgatcgaga cgatttataa gagactgact gccgctgatc
     1381 cgtcaatctc gaagactgtt ctggcagaaa aggtattcgg cggaaaactg cgcattatct
     1441 ttacaggtgg tgcacatctg gatccgtact atattgaccg ttttgcagaa tatggcgttg
     1501 aagtactgga aggctatgga atgtcagaat gctctcctgt tatcagcaat aatacaccgg
     1561 aaaaccataa aaaaggttct atcggaaaac cacttgaaaa tgcagagatc agatttgaaa
     1621 acggtgagat cctggtaaag ggaagcagtg ttatgaaggg atattatcag atgcctgatg
     1681 agacagcaga gactttaaag gatggctggc tgcataccgg tgacaaggga tatatggatg
     1741 aagacggtta tctgtttatc aatggacgtg tgaagaattt gatcatcctg tccaatggtg
     1801 aaaatgtatc tccggaggag attgagaata agctggctct gaatccgctg ataggagaag
     1861 tgatcgtcac aggtgaggac aatggactga ccgcccgcat ctatccggag caggctgttg
     1921 tggaagcgaa agcattggat gcagaggcaa ttcaggcaca gcttcaggca tttctggatg
     1981 aatataacag aaatcagccc acctatcgcc gcattacagg attggttgtc cgcaaaaatc
     2041 cgtttatccg caacacaaca aaaaaaatca ggagacagga tgtcctgatt gatgagcctc
     2101 tggaataaga cagaggctta agataaaagt ggaagcatac cggataaaaa aacctcagac
     2161 agaatatcag cgatcacttc cggtgccaga ttaaaattat cccggctcca tttgtggaga
     2221 acgccgatgg tactgccgat ggcgcaggtg atcacatagg aaacttcttc ctttgtatgc
     2281 ctgtcaggcc tggaattaga tgtagtttct gtaacataat gatggagcat agtcagcatt
     2341 ttttcaaaca gatcattgcc atgaggcgtg ctcaaaagac cggacagaaa aggattttcc
     2401 atagtgtatc ggcacagagt cagaaaatag gaactgcaga tatcccggtc attttttggc
     2461 tggaaatttt ccatcatgga aaaaacatca tgaatagttt tatcctcaac tgcagagatc
     2521 agtgaaggga tgtcctgata gtgattataa aaggtacttc tggcaatacc agctgtttta
     2581 gtgat
//
LOCUS       DBMDXG010000320         2583 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8679_length_2583_cov_1.187752, whole genome shotgun sequence.
ACCESSION   DBMDXG010000320 DBMDXG010000000
VERSION     DBMDXG010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8679_length_2583_cov_1.187752"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="ACZPD3_08495"
     CDS             <1..335
                     /locus_tag="ACZPD3_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1416059.1"
                     /translation="TAVVGSMLNIKPVLHVDNDGKLTAIGKVRGRKKSLQELVKLMDE
                     KIGSYHDTCHTIFISHGDCEEDANYVAEKVKEKYQINTIIMNQVGATIGAHSGPGTMA
                     LFFVGDER"
     gene            431..>766
                     /locus_tag="ACZPD3_08500"
     CDS             431..>766
                     /locus_tag="ACZPD3_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR1416060.1"
                     /translation="MKLLKKAFAFTFIFMLSVSGLTGYQVNASEEQKHLDILFTHDLH
                     SHLNSFQTIVDGTQQETGGFARLKTLINEHEKENTDTLILDGGDFSMGTLIQTVYDTE
                     SAELRMLGYL"
     assembly_gap    767..776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <777..2361
                     /locus_tag="ACZPD3_08505"
     CDS             <777..2361
                     /locus_tag="ACZPD3_08505"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR1416061.1"
                     /translation="LGNHEFDYGSDGLADMLNAAVSSGENLPRMVVCNVDWDAMKKAG
                     LSEGQKQIYEAFQTYGVKDYTVIQKGDVKIAVLGVFGKDSLDCAPTCELLFKDPSEAA
                     RETVEEIKKNEDVDMIACVSHSGTWEDEKVSEDEILAKNVPDIDLIVSGHTHTQLTEP
                     ILQGDTCIVSCGEYGKNLGTLSMTQKENGRWEPDTYELVPVTDKIKADEATQKKIDEL
                     SDTVDTNYLSNFGYTREEILAENDIEFNSLSEMETKHEELNLGDIISDAYVYAVENTG
                     DSDGEKVDVAIVPAGTVRDTYTKGDITVEQVYNSFSLGTGKDGLAGYPLISAYLTGKE
                     LKTVAEVDASISDFMTIARLYCSGMNFTYNPHRMILNKVTDCYLTGKDGEREEIQDDK
                     LYHVVTDLYTGRMLGSVLDKSYGLISIVPKDKNGNPIENLEDYAVTDGKKELKAWAAI
                     AEYMQSFEDTDKDGIANVPEYYDTTHERKVIDNSKNIINLVKYPNKFAVAIVGICIVT
                     VGVILLLIFGVVKIIRRRR"
BASE COUNT          864 a          454 c          609 g          646 t
ORIGIN      
        1 ctactgcagt agtaggaagc atgctgaata ttaaaccggt ccttcatgta gacaacgacg
       61 gaaaactcac tgccatcggt aaagtccgcg gccgtaaaaa atcccttcag gagctggtaa
      121 agcttatgga tgagaaaatc ggcagctatc atgacacttg ccataccatc tttatcagtc
      181 atggcgactg cgaagaagat gcaaattatg tggcagagaa agtaaaagaa aaataccaga
      241 tcaacacgat tatcatgaat caggtaggag ccaccatcgg cgctcactca ggtccgggaa
      301 caatggcgtt gttctttgtg ggagatgaaa gataaattag ttttttttgt aaaatggttt
      361 catggtatca ttatcctgcg ggaagataaa tgatactgtg gaaccacttt tgatattaca
      421 ggagaatttt atgaaattat taaaaaaagc attcgcattc acatttatat ttatgctgag
      481 cgtttcagga ctgacaggat atcaggtaaa cgcctcagag gagcaaaaac atctggatat
      541 cctgttcacc catgatctgc attctcattt gaacagtttt caaacgattg tagacgggac
      601 acagcaggaa accggcgggt ttgcccggct gaaaacattg atcaatgagc acgagaaaga
      661 gaatacagat acactcatac tggatggcgg agacttctcc atgggaactc tgatacagac
      721 tgtatatgac acagagtccg ccgaactgcg gatgcttgga tatctgnnnn nnnnnntctg
      781 ggaaatcatg aatttgatta cggctccgat ggtctggcag atatgctgaa cgccgcagtc
      841 agttcaggcg aaaaccttcc caggatggtt gtgtgtaatg tggactggga tgcaatgaaa
      901 aaagccggcc tcagtgaagg gcagaaacag atttatgaag catttcagac atatggagta
      961 aaagactata cggtaattca aaaaggtgat gtaaagattg cagtactggg tgttttcggc
     1021 aaggattctc tggattgcgc tcctacctgc gagcttcttt tcaaagaccc gtcagaagca
     1081 gccagagaaa cagtagagga aataaaaaag aacgaagatg tagacatgat cgcctgtgta
     1141 tcacacagtg gaacatggga ggacgaaaaa gtatcagagg acgaaatcct tgcaaagaat
     1201 gttccggaca ttgatctgat cgtcagcgga catacccaca cacagttgac agaaccaatc
     1261 ctgcagggag atacctgcat cgtatcctgt ggagaatatg ggaaaaatct gggaactctt
     1321 tccatgactc agaaagagaa tggacgatgg gaaccggata cctatgagct tgttccggtt
     1381 acggataaaa tcaaggcaga tgaagccaca cagaagaaaa tagatgaatt gtcagatact
     1441 gttgacacca attatctttc caattttggt tacaccagag aggaaattct ggcagagaat
     1501 gacattgaat ttaacagcct cagtgagatg gaaacaaagc atgaggagtt aaatctggga
     1561 gatatcatat cagatgctta tgtatatgca gtagagaata caggagactc tgacggagaa
     1621 aaagtagatg ttgcaatcgt acctgccgga actgtaagag atacttatac aaaaggcgat
     1681 ataacagtgg agcaggtata taattccttc tcgctgggaa caggaaagga cggacttgca
     1741 ggttatcctc tgatcagtgc atatttaacg ggaaaagaac tgaaaacagt ggcagaggta
     1801 gatgcctcaa tttcagattt tatgacaata gccagattat attgcagtgg aatgaatttc
     1861 acctataatc cccaccgcat gatcttaaat aaagtaacag actgttatct gacaggcaag
     1921 gacggggaaa gggaagaaat acaggacgat aaattatatc atgttgtcac cgacttgtac
     1981 actggccgta tgcttggttc tgttctggac aaatcctatg gtctgatatc catcgtcccc
     2041 aaagataaaa acggcaatcc catagagaat ctggaagatt atgcagtcac ggatggaaaa
     2101 aaagaactga aagcctgggc tgcaattgca gagtatatgc aatcctttga agacacagac
     2161 aaagacggaa ttgcaaatgt gccggaatat tatgatacaa cacacgaaag aaaagttata
     2221 gacaacagca aaaacatcat aaatctggta aaatatccga ataaatttgc cgtagcgatt
     2281 gtcgggatct gtattgtgac tgttggggtg attctgctgt tgatctttgg ggttgtaaag
     2341 attatcagaa gaaggagata gtattttact ataaacatag ccacacatgt gattgcaaaa
     2401 ggcttccggg atgcatatga tattgctgta tatagttata ctggaaagta gcgaatattc
     2461 aattagtgaa tgaaaatgta gacatattgt ggatgcagat ttctgcattt tgtgttagga
     2521 tagagtagta aatacaactt aagaattgta aagatgattc gcagattatt tatttcaaac
     2581 att
//
LOCUS       DBMDXG010000321         2576 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8709_length_2576_cov_1.320404, whole genome shotgun sequence.
ACCESSION   DBMDXG010000321 DBMDXG010000000
VERSION     DBMDXG010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8709_length_2576_cov_1.320404"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="ACZPD3_08510"
     CDS             complement(<1..638)
                     /locus_tag="ACZPD3_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416062.1"
                     /translation="MRKSDWAKWLIVIPAMLGIVLVTYVDRITDIWGFGAFICQLIAI
                     LELAYGMRIAMLAQSRKKSYRLTPEERHEYAQYLYEKQYQRYPAVANQMLLVMARMSI
                     LLDNYERATQELEDICIDKFNPAQLKVYYYLKVVTAVVAGDTEGLQESQMRYAGIPDT
                     KCVYPPETELAEWIEQRDTVQMAEALKKAVSDKKEHPVRISIITLILAYSTF"
     gene            complement(845..2098)
                     /locus_tag="ACZPD3_08515"
     CDS             complement(845..2098)
                     /locus_tag="ACZPD3_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1416063.1"
                     /translation="MEIKRDAYLQQLIERKDNGMIKVITGIRRCGKSFLLFTIFKRYL
                     LENGIGSDHIIEIALDGIENEELRDPKMCFKYIKDAVKDDGKYYLLLDEVQFMPRFEE
                     VLNSLLRMSNIDVYVTGSNSRFLSSDIVTEFRGRGDEIRIYPLSFAEFYSVYDGDYDD
                     AWDDYMIYGGLPQVVGFQSERQKADYLKNIFANVYLKDVIERNKIQNVDEIGILVDVL
                     ASAIGAPTNPSKIANTFASQRQMTYTNKTISNHIDYLADAFLISKASRYDIKGRKYIG
                     ANLKYYFTDLGLRNARLNFRQQEPTHIMENIVYNELLIRGYNVDVGVVDIFDKDKEGK
                     RVRKQLEVDFVVNQGNQRYYIQVAYDMTSEEKQTQEFNSLRNIPDSFKKIVIVNGSKK
                     PWRNDEGFVIMGMKYFLLNANSLEF"
     gene            2260..2469
                     /locus_tag="ACZPD3_08520"
     CDS             2260..2469
                     /locus_tag="ACZPD3_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1416064.1"
                     /translation="MILPLQAIVGFFPNPERKGNIHYFSDNELEVLRIIECLKKSGLG
                     IKDIKQFFIWVSEGNSSYEKKKRII"
BASE COUNT          765 a          612 c          417 g          782 t
ORIGIN      
        1 aagaaggtac tatatgcaag aatcaaagta atgatactga tccgtaccgg atgctctttc
       61 ttatccgaca ctgccttttt cagtgcttct gccatctgca ctgtatctct ctgctctatc
      121 cactcagcaa gctcagtttc tggcggatat acacatttag tatccggaat ccctgcataa
      181 cgcatctggc tctcttgaag cccttctgta tctcctgcca ctactgctgt aaccactttc
      241 aggtaataat acaccttcaa ctgtgccgga ttaaatttat ctatacaaat atcttccagc
      301 tcctgtgtag cccgttcata attgtcaaga agtatactca tccttgccat aaccaaaagc
      361 atctgattcg ccacagcagg gtaacgttga tattgtttct catataaata ctgtgcatac
      421 tcatgtcgtt cttccggtgt taaccggtag gacttctttc tactctgtgc cagcatcgcg
      481 attcgcatac cataagccaa ttctaaaatc gcaatcagct ggcagataaa tgcaccaaat
      541 ccccagatat ctgttatacg atccacatag gttaccaaca caatgcctag cattgccgga
      601 atcacgatca accattttgc ccaatctgat tttctcacac taacactctc ctgaatagtt
      661 tttctgaata tagtatatta ccgcacacac caatcaacca taatctgcgg aacagtctcg
      721 tttgtacgga taaaaaaata aactgagcct cggctgttcc aacatgaaat cagccgtggc
      781 cttatttata tcatttcact cttttttgaa atagaacaac accacagcaa caactcaaat
      841 cgacttaaaa ttccagacta ttcgcattca gcaagaaata cttcattccc atgatgacaa
      901 aaccttcatc atttctccaa ggctttttgc tgccgtttac aatgacgatc tttttgaaag
      961 agtccgggat attacgcaga gaattgaact cctgcgtctg tttttcttcc gaagtcatgt
     1021 catatgcaac ctggatatag tatctctggt ttccttgatt caccacaaag tcaacctcca
     1081 actgcttgcg aacacgtttg ccctctttat ccttatcgaa gatatccacg acacccacat
     1141 caacattgta gccacggatc agcagctcgt tataaacaat gttctccatg atatgagtag
     1201 gctcctgctg tctaaaattc aagcgagcat ttctcagtcc cagatcagtg aaatagtatt
     1261 tcagatttgc accgatatat tttcgtccct taatgtcgta acggcttgcc ttggaaatca
     1321 aaaacgcatc tgccaaatag tcaatatggt ttgagatagt tttattggta taagtcatct
     1381 gacgctgact ggcaaaggta ttcgcaatct ttgaagggtt ggtcggtgcg ccgattgcag
     1441 atgcaagcac atcaaccaga ataccaattt catccacatt ttgaatttta tttctttcta
     1501 taacatcctt cagataaaca ttagcaaaaa tgttcttcag atagtctgct ttctgtcgtt
     1561 cactctggaa accaacaacc tgcggcaatc ctccgtagat catgtagtca tcccaggcat
     1621 catcataatc accgtcatag acagaataaa actcagcaaa ggagagagga tagattctga
     1681 tttcatcacc tctgccacgg aactcggtca caatgtcact ggacaggaac ctggaattac
     1741 ttccggtgac atacacatca atgttgctca tgcgtagcaa actgttcaga acttcctcaa
     1801 atcggggcat gaactgtact tcatccagaa gaagataata cttcccatca tctttcacag
     1861 catccttgat atacttaaaa cacatcttgg gatctcttaa ttcctcattc tctataccgt
     1921 ccaatgcaat ttcaataata tgatcagagc caatgccatt ctccagtaaa tatctcttaa
     1981 agatggtaaa cagcaaaaag gattttccgc atctacgaat gccggtgatt acctttatca
     2041 ttccattatc ttttcgctca atcaactgct gcaggtaagc gtctctttta atctccatca
     2101 tcactctccc cgtttctgtg catctgcact ttttttagta atgatattct atcataaaac
     2161 aagatacgtt tcaatattca ttcctatttt atgtgtatat gcatattttt atgtaatgta
     2221 ctaatacaaa tataagtcgc cttagctgcc ttagcgtttg tgatccttcc attgcaggcc
     2281 attgtaggct ttttccctaa tccggaacgt aagggaaaca tccattattt cagcgataat
     2341 gaattggaag tgcttcgtat tattgaatgc ctgaagaaat cagggcttgg gatcaaagat
     2401 ataaagcaat tttttatctg ggtatctgaa ggcaactcca gctatgaaaa aaagaaaaga
     2461 attatttgaa gcccgaaaat cagccgttga aactgaaatc caagaacttc aaaaaaactc
     2521 tctccctttt aaaatccaaa tgctggtatt atgaaacagc aatgaaagat gataag
//
LOCUS       DBMDXG010000322         2574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8716_length_2574_cov_2.419733, whole genome shotgun sequence.
ACCESSION   DBMDXG010000322 DBMDXG010000000
VERSION     DBMDXG010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8716_length_2574_cov_2.419733"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="ACZPD3_08525"
     CDS             complement(<1..184)
                     /locus_tag="ACZPD3_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416065.1"
                     /translation="MKMIADLHIHSRFSMATSKEGTPENLDFWARKKGISLIGTGDFT
                     HPVWREELKERLVSEGN"
     gene            complement(261..848)
                     /locus_tag="ACZPD3_08530"
     CDS             complement(261..848)
                     /locus_tag="ACZPD3_08530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR1416066.1"
                     /translation="MDMDKTEKNQEKFTCGEFEYQVIRSARKTMTLEVRRDGNVIVRA
                     PLRTGLPRIKRFVNQKQEWVLGCLERTKEYREQKPLSADLSESKRNVYIRKAKETITK
                     RVSYFARLMGVSYRNITIREQKTRWGSCSSEKNLNFNWKLILAPPEVLDYVVVHELCH
                     LKEMNHSKAFWDEVGKVMPEYETYKLWLKENGWKL"
     gene            complement(908..1711)
                     /locus_tag="ACZPD3_08535"
     CDS             complement(908..1711)
                     /locus_tag="ACZPD3_08535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1416067.1"
                     /translation="MDKLLDLTRDFDAAKAAFEAYLDEYDRADDKIHLKIVHTYGVVD
                     AAEDIARRMGLGEEDVQLAKVIGLLHDIGRFEQIKRFDSFEPGTMEHAVYGAQLLFGP
                     EKMIRRFVKDDRFDSLICTAIEKHSDFKLEGITDERTLLHAKLIRDADKLDNCRVKLE
                     ESMETLLGVDEKGVGEGVIAPKVWESCMAKESVLSSDRVSKVDYWISYIAQYYDINFP
                     ETYEIMREHDYVKRIMDRVPYALPETQEKMDILAAEMGEYMDERIRNKK"
     gene            complement(1751..>2574)
                     /locus_tag="ACZPD3_08540"
     CDS             complement(1751..>2574)
                     /locus_tag="ACZPD3_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3878 family protein"
                     /protein_id="HIR1416068.1"
                     /translation="LKNARMTGNYVRDYEGEFEGSIEKADRDLCEAEYILVIHQGQNV
                     FTVFFEDILLETQLYNYGELGHFWVKGYENLRVMEYQIAILRDKYEYLGEKYCTEYEG
                     KLAMLRDFPPLNYLFYPAVPEKYIVPVDNPWEVTAEALAVMQELATEAGDEKLGKMLR
                     RYEKNSDISNAKKIAGMLCRSSHLPVITLLGEKIREAASVYPDRDFGRKQNKYLHELM
                     EKAERRKEELEAENVQTLIYREEPFIYDCDSISFQVYLMIVRKGVWKQKIMVEKI"
BASE COUNT          621 a          697 c          381 g          875 t
ORIGIN      
        1 cgtttccctc tgaaacaagt ctctccttca gttcttctct ccataccgga tgcgtgaaat
       61 cacctgttcc aatcagtgaa attcctttct ttctcgccca gaaatccaga ttttcaggcg
      121 tgccctcttt gcttgtcgcc atggaaaatc tggaatgaat gtgcagatca gctatcattt
      181 tcattaacat ttatcccctt aaattcacat caaagtattt cttttccaga gttataaaat
      241 atcaacaccc aaagatatat ttataacttc catccgtttt ctttcagcca caacttgtac
      301 gtctcatact ccggcattac tttcccgacc tcgtcccaga atgccttcga atgattcatt
      361 tccttcagat ggcagagttc atgtaccact acataatcca gcacttccgg aggcgccagt
      421 atcagtttcc agttaaagtt cagattcttc tcactgctac agcttcccca tcttgttttc
      481 tgttctctga tggtgatatt gcggtaagaa acgcccatga gtctggcaaa ataagaaact
      541 cttttggtaa tcgtttcttt tgcttttcgt atatagacat ttcgtttcga ttcgctcaga
      601 tctgcgctca gcggcttctg ttcccggtat tcttttgtcc gttccagaca tcccagaacc
      661 cactcctgct tctggtttac aaatcttttg atcctcggca gacctgtacg gagaggtgcg
      721 cgaacgatta cgtttccatc cctgcgaacc tccagagtca tggtttttct ggcacttctt
      781 atgacctgat attcaaattc tccgcaggtg aatttctcct gattcttctc tgttttatcc
      841 atatccattt tgttcactct tgtcttttca tatgtcccca cccactgttt attgcttttc
      901 gcaattttta ttttttgttt cgaattctct catccatata ttcccccatc tctgctgcca
      961 gaatatccat cttttcctgt gtttccggca atgcataagg aaccctgtcc atgatccttt
     1021 tcacataatc atgttcacgc atgatctcat aggtttccgg gaaattgatg tcataatact
     1081 gagcaatata ggaaatccag tagtccactt ttgaaactct gtctgaagat aatacagatt
     1141 cctttgccat acaggactcc catacctttg gtgcgattac tccttcacca acacccttct
     1201 catccactcc cagaagtgtt tccattgact cttccagctt taccctgcaa ttatccagct
     1261 tgtctgcatc tcggattaac tttgcatgaa gcagggttct ctcgtccgta attccttcca
     1321 gtttgaaatc actgtgcttc tcaatcgcag tgcagatcaa actgtcaaac ctgtcatcct
     1381 tcacaaatct gcggatcatt ttctccggtc cgaacagaag ctgtgcacca tatactgcat
     1441 gctccatggt tcccggttca aagctgtcaa atcgtttaat ctgttcaaat cttccaatat
     1501 catggagcaa tccgattacc ttcgccagct gtacatcttc ctcacccagc cccatacggc
     1561 gggcaatgtc ctctgctgca tcaaccactc cataagtatg tacgatcttc agatgaatct
     1621 tatcatctgc tctgtcatat tcatccagat atgcttcaaa agcagctttc gctgcatcaa
     1681 agtctcttgt taagtctaat aatttatcca ttattattat aatcccttct ttattcctgt
     1741 tcggactact ttatattttc tcaaccatta ttttctgctt ccatacacct tttctcacaa
     1801 tcataagata tacctgaaac gatatggaat cacagtcata aatgaaaggt tcttctctgt
     1861 agatcagagt ctgaacattc tctgcttcca attcttcttt tcttcgttca gctttttcca
     1921 tcaattcatg tagatattta ttctgttttc tgccaaaatc tctgtccgga tagaccgatg
     1981 ctgcttccct gattttttct cccagcagag taatcaccgg taaatgactg ctcctgcaaa
     2041 gcattccggc tatctttttg gcatttgata tatccgaatt tttttcatag cgcctgagca
     2101 tttttcccag tttttcatct cctgcttccg ttgccagttc ctgcataact gccagtgctt
     2161 ccgccgttac ctcccatgga ttgtccaccg gaacaatata cttctccggt actgccggat
     2221 aaaaaagata atttaacggt gggaaatcac gcagcattgc cagctttcct tcatactcag
     2281 tgcaatattt ttctcccaga tactcatatt tatcacgcaa tatggcaatc tgatactcca
     2341 ttaccctgag attttcatag cccttcaccc agaaatgtcc cagctctcca taattataca
     2401 actgtgtttc cagcaaaata tcttcaaaga atacagtaaa cacattctgc ccctgatgaa
     2461 tcaccaaaat atactctgcc tcacataaat ccctgtccgc tttctctata gagccctcaa
     2521 actctccttc ataatccctg acataatttc ccgtcatcct ggcatttttc agaa
//
LOCUS       DBMDXG010000323         2570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8737_length_2570_cov_1.528554, whole genome shotgun sequence.
ACCESSION   DBMDXG010000323 DBMDXG010000000
VERSION     DBMDXG010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8737_length_2570_cov_1.528554"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(48..842)
                     /locus_tag="ACZPD3_08545"
     CDS             complement(48..842)
                     /locus_tag="ACZPD3_08545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR1416069.1"
                     /translation="MRLCSIASGSSGNCIYVGSDNTHLLVDVGISGKRVEQGLNSLEL
                     TGKDIDGILITHEHSDHIKGLGVIARKHQIPVYATEGTVEALSHMNLGKMPEGIFREI
                     HEDEPFEINDLTVNPFTIPHDAAQPVGYRVECGEHSVGIATDLGKYNEYIVGNLQNLD
                     ALLLEANHDIRMLQVGKYPYYLKQRILGDRGHLSNENAGRLLCRLLHDNMKGIFLGHL
                     SRENNYEELAYETVCSEVTLGDNPYKSRDFRIQVAQRDCISEVITV"
     gene            complement(829..1431)
                     /gene="coaE"
                     /locus_tag="ACZPD3_08550"
     CDS             complement(829..1431)
                     /gene="coaE"
                     /locus_tag="ACZPD3_08550"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161325.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR1416070.1"
                     /translation="MMITIGITGGVGAGKSTVLDFLEEKYQAYVMKADEIGHLVMEPG
                     QSCYEPVIALFGRQIIKNDKTIDRRQVSDVVFSHPELLEKLNQIIHPAVKQYIREQLA
                     VKKQQEQKICVVEAALLLEDHYQEFCDTIWYIHTDEEIRIRRLMENRGYTREKSVSII
                     ASQAPETFFRENADYVVVNNGDFAQTRRQIEEGIRKYETL"
     gene            complement(1431..>2570)
                     /locus_tag="ACZPD3_08555"
     CDS             complement(1431..>2570)
                     /locus_tag="ACZPD3_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR1416071.1"
                     /translation="KSEEVKSYGEKLSVRIGELEKQIWQQAGEEFNINSPKQMGVILF
                     EKLGLKGGKKTKTGYSTAADILEKLAPEYPIVKDILEYRQLAKLKSTYADGLVGEIAE
                     DGRIHSTFNQTITATGRISSTEPNLQNIPVRMELGRLIRKVFVPEEGYVFLDADYSQI
                     ELRVLAHMSGDEKLIQAYKEAQDIHRHTASEVFHVPFDEVTDLQRRNAKAVNFGIVYG
                     ISSFGLSQDLSITRKEAAEYIERYFETYPKIKGFLDGLVEEGKEKGYVTTMFGRRRPI
                     PELKSGNFMQRSFGERVAMNSPIQGTAADIIKIAMNRVYRRLKDENLKSRLVLQVHDE
                     LLIETCKEEIPQVSAILEEEMKGAAKLSVELEVDMHQGNNWYEAK"
BASE COUNT          627 a          680 c          439 g          824 t
ORIGIN      
        1 ttcctactac tgtgatgatt gtctttttca taagttcttt gtcctttcta cactgtaatt
       61 acttctgata tgcaatccct ctgtgccacc tgaattctga aatcacgcga cttataaggg
      121 ttgtcaccta aggttacttc tgaacagacg gtttcatatg ccagttcctc atagttattt
      181 tccctgctca gatgtccaag gaaaatccct ttcatattgt catgcagcag cctgcataaa
      241 agcctgcctg cattctcatt ggaaagatgt cccctgtctc caaggattct ctgtttcaga
      301 taatagggat attttccaac ctgcagcata cggatgtcat gatttgcttc cagaagaagc
      361 gcatccagat tctgcagatt accgactatg tactcattat attttccaag gtctgtagca
      421 atccccacag aatgctctcc acactctacc cggtatccta caggctgtgc agcatcatga
      481 gggatcgtaa aaggattcac tgtcagatca ttaatctcaa aaggctcatc ttcgtgtatc
      541 tcacgaaaaa tcccttccgg catttttcca agattcatgt gagataaagc ttccacagtt
      601 ccttctgtcg cataaaccgg tatctgatgt ttccttgcga tcacgcccag acctttgata
      661 tgatccgaat gttcatgtgt aatcaagata ccatcaatat cttttcctgt caattccaga
      721 gaattcaatc cctgttctac tctttttccg ctgatgccta catccaccag aagatgggta
      781 ttatctgagc ccacataaat acagtttccg ctgcttccgc tggcaatgct acaaagtctc
      841 atattttctg attccttcct caatctgccg tctggtctgt gcaaaatctc cgttatttac
      901 cacaacatag tctgcatttt cccgaaaaaa agtctccggt gcctgactgg caatgatact
      961 gactgatttc tctcttgtat aaccgcggtt ctccatcagt ctcctgatac ggatctcttc
     1021 atccgtatga atataccata tcgtatcgca aaactcctga taatgatctt ccagaaggag
     1081 tgcagcttcc accacacata tcttctgttc ctgctgtttc tttacagcta actgctctct
     1141 gatgtactgt ttcacagccg gatggatgat ctggtttaat ttctccagaa gctctggatg
     1201 tgaaaatact acatccgaaa cctgtctgcg gtcaatagtt ttatcatttt ttatgatttg
     1261 tcttccaaat agtgcaatca ccggttcata acaggactgc cccggctcca tcaccagatg
     1321 cccgatctca tctgctttca tcacatatgc ctgatacttt tcttcaagaa aatcaagcac
     1381 tgtacttttt ccggcaccca ctccgccggt aattcctatt gttatcatca tcacttcgcc
     1441 tcataccagt tattaccctg atgcatatcg acttccagtt ctacggaaag ctttgcggca
     1501 cctttcatct cttcttcaag aatagcagaa acctgcggga tttcctcttt acaggtctct
     1561 atcagaagtt catcgtgtac ctgaagtaca agacgggatt taagattctc atcctttaat
     1621 cgtctgtaca cacggttcat tgcaatttta atgatatccg ccgcagttcc ctgtattgga
     1681 gagttcatgg ctactcgctc cccaaaagac ctctgcataa aattaccgga tttcagttcc
     1741 gggatcggcc tgcgtctgcc aaacatggta gtcacatatc ctttttcttt tccctcttcc
     1801 accagtccgt caagaaaacc tttgatcttt ggataagttt caaaatatct ttcaatatat
     1861 tcggctgctt cttttcgtgt gatacttaaa tcctggctga gcccaaagga actgatccca
     1921 taaacgattc caaaatttac tgccttggca tttctcctct gcagatctgt cacctcatca
     1981 aagggcacat ggaatacctc tgatgctgta tgtctgtgga tatcctgggc ttctttatat
     2041 gcctggatca gtttctcatc ccctgacata tgcgccagaa cacgaagctc gatctgggaa
     2101 tagtccgcat ccagaaatac atatccctct tccggaacaa ataccttacg gatcagtctt
     2161 ccaagctcca ttcttacagg gatattctga agattcggct ctgtgctgct gatccttcct
     2221 gttgctgtaa ttgtctggtt aaaagtagaa tgaatcctgc cgtcctctgc gatctcacca
     2281 acaagcccgt ctgcataagt agatttcagt ttcgcaagct gacggtactc cagaatatcc
     2341 tttacaatgg gatactccgg tgcaagcttt tccagaatat ctgccgctgt ggaataacct
     2401 gtctttgttt tctttccacc cttaaggcca agcttttcaa acaggatcac tcccatctgt
     2461 ttcggagaat tgatattaaa ctcttcccct gcctgctgcc agatctgctt ctccagttct
     2521 ccaatacgta cacttagttt ctcaccgtag cttttcactt cttcactctt
//
LOCUS       DBMDXG010000324         2566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8754_length_2566_cov_1.776065, whole genome shotgun sequence.
ACCESSION   DBMDXG010000324 DBMDXG010000000
VERSION     DBMDXG010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8754_length_2566_cov_1.776065"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="ACZPD3_08560"
     CDS             <1..984
                     /locus_tag="ACZPD3_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR1416072.1"
                     /translation="YLDEEHIEWLKRYLQDYENAFILISHDIPFLNSVINLVYHMENQ
                     ELNRYVGDYDHFQEVYEVKKAQLEAAYRRQQQEISELKDFVARNKARVSTRNMAMSRQ
                     KKLDKMDVIELAAEKPKPEFHFKYGRTPGKYIFETKDLVIGYNEPLSRPVTLSMERGN
                     KIALVGANGIGKTTLLKSILGLIPSLKGSCELGENLQIGYFEQEVKGDNKTTCIDEIW
                     AEFPSYTQYEVRSALAKCGLTTKHIESQVRVLSGGEQAKVRLCKLVNKETNILLLDEP
                     TNHLDVDAKEALKQALIEYKGSILLICHEPEFYRDVVSQVWDCSKWTTKVL"
     gene            1012..2376
                     /locus_tag="ACZPD3_08565"
     CDS             1012..2376
                     /locus_tag="ACZPD3_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR1416073.1"
                     /translation="MSKAKTVDLTSGPILKTLAELALPIMISSMLGTAYSIMDMAWIG
                     LLGAKAVAGVGVGGMYVWLSQGLASLSRMGGQVNTAQACGRKDYDQARSYAAGSLQLT
                     ALSGILFAAVCVIFIQPLLGFFNLTDAETYTAAKSYTLITCGLILFSYLNLTLTGLST
                     AQGDSRTPLLANFIGLAGNMILDPLLILGIGPFPRLEVAGAAIATVSSQILVFVVMVL
                     RIRHSRLEPNVLQHLNLLSVFPKEYYKHIFRIGFPTAIQGSIYCFISMILTRMVSGYG
                     AAAIATQRVGGQIESVSWNTADGFASALNAFIAQNYGARKKDRIRKGYSLSFRVLTIW
                     GLFVTAAFVFLPEPIARLFFHEKEALDTAVNYLVIIGFSEVFMSIELMTVGALSGLGR
                     TRLSSTISVILTGSRIPLALILTHAGMGLNGVWWALTISSIVKGIVFTLTFRHISRRL
                     PEKV"
BASE COUNT          651 a          527 c          639 g          749 t
ORIGIN      
        1 tatctggatg aggaacatat cgaatggctg aagcgttatc tgcaggatta tgagaatgca
       61 tttattctta tctcccacga tattccgttt ctgaattctg ttatcaatct ggtttatcat
      121 atggagaatc aggaactgaa ccgttatgtc ggggattatg accatttcca ggaagtgtat
      181 gaggtgaaga aggctcagct tgaggctgct tacagacgcc agcagcagga aatcagtgaa
      241 ctgaaggatt tcgtcgcaag aaataaggcc agagtttcta caagaaatat ggcaatgtcc
      301 agacagaaga agctggataa gatggatgtg atcgagcttg ctgccgagaa gccgaagcct
      361 gagtttcatt ttaaatacgg acgcactccg gggaaatata ttttcgagac gaaggatctg
      421 gttattggtt ataatgaacc tctttccaga cctgttactc tctctatgga aagaggaaat
      481 aagattgcgc tggttggtgc aaacggtatc ggtaagacaa ctttactgaa gagtattctg
      541 gggttgatcc cgtctctgaa gggttcctgc gaactcgggg agaatcttca gattggatat
      601 tttgagcagg aagtaaaggg tgataacaag actacctgta ttgatgagat ctgggcggag
      661 tttccgtctt atactcagta tgaggtgcgg tctgctctcg ccaaatgcgg tctgactacc
      721 aagcatattg aaagtcaggt acgtgtactg agtggcgggg aacaggcgaa ggtgcgtctg
      781 tgtaagctgg tgaataaaga aacgaacatt ctgcttctgg acgagcctac caatcatctg
      841 gatgtggatg caaaagaagc tttgaaacag gcattgatcg aatataaggg aagcattctc
      901 ctgatctgcc atgaaccaga gttttaccgc gatgtggttt ctcaggtatg ggactgcagt
      961 aaatggacta caaaagtatt ataataatta gtatatgaag ggttattaca aatgagtaaa
     1021 gcaaaaactg tggatctgac ttccggtccg atcctgaaaa ctctggcaga gctggctctg
     1081 cctatcatga tttcttccat gctgggaaca gcatacagta ttatggatat ggcatggatc
     1141 ggactgcttg gtgcaaaggc ggttgcaggc gtgggagtag gcggaatgta tgtctggctc
     1201 tcccaggggc ttgcatccct gtcaagaatg ggcggacagg taaatacagc tcaggcatgc
     1261 gggcgcaaag attatgacca ggcacgttcc tatgctgccg gttctctgca gctcactgca
     1321 ctgtccggca ttttgtttgc tgctgtctgt gttattttca ttcagcctct gctgggattt
     1381 tttaatctga ccgacgcaga aacttatact gctgccaaat cttatacatt gatcacctgt
     1441 ggactgattc ttttttctta tctgaatctg acgctgacag gactctctac tgcgcaggga
     1501 gattcccgca ctcccctgct tgcaaacttt atcgggcttg caggaaatat gattctggat
     1561 ccgctgctga tccttggcat cggacctttc ccgaggctgg aggttgccgg agccgctatt
     1621 gcgacagtaa gttcacagat tctggttttt gtagtgatgg ttctgcgtat ccggcattcc
     1681 agactggagc caaatgtttt acagcatctg aatctgctgt ctgtttttcc aaaagaatat
     1741 tataagcaca ttttcaggat cgggtttccc acagctatac agggaagcat ttactgtttc
     1801 atctccatga ttctgacccg tatggtttct ggttatggtg ctgccgctat tgcaacgcag
     1861 cgtgttggcg gacagattga atccgtatcc tggaatactg ctgatggttt tgcttcagca
     1921 ctgaatgctt ttattgccca aaactacggt gcacgaaaaa aagaccgtat ccgtaaaggt
     1981 tacagtctgt catttcgcgt tctcacaatt tggggcctgt ttgtcactgc agcttttgtc
     2041 tttcttccgg aaccaattgc caggttattt ttccacgaaa aagaagctct ggatactgct
     2101 gtcaattatc tggtcattat cggattcagt gaagtcttta tgtccattga gctgatgaca
     2161 gtgggcgcat tatcaggact tggacgcacc agattatcca gtacgatcag tgtgatcctg
     2221 acaggctcac gaatcccgct ggcattgatc ctgacacatg cgggaatggg attaaatggt
     2281 gtatggtggg cgcttacaat ctcttctatc gtgaagggaa tcgtgtttac actgacattc
     2341 cggcatatca gcaggcggtt gcctgaaaag gtctaaaggt ctcttaatat agctgtcaaa
     2401 ctcaaaagag gactgttcag aaaaatttct gtcagtcctc tttttactgt ttctattatt
     2461 tcgcaacaaa gtttacgatc ttgttcggta cataaatctc tttgataagg ttcttacctt
     2521 ccaggaagga agcaattctc tcgtctgctt tggcgatctc gaatgc
//
LOCUS       DBMDXG010000325         2563 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8764_length_2563_cov_2.927295, whole genome shotgun sequence.
ACCESSION   DBMDXG010000325 DBMDXG010000000
VERSION     DBMDXG010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8764_length_2563_cov_2.927295"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="ACZPD3_08570"
     CDS             complement(<1..302)
                     /locus_tag="ACZPD3_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR1416074.1"
                     /translation="MDREEVLCPPFLLEKDVKCSMENMIAVLVIVILVGSAAAYLIKA
                     KRSGVKCVGCPAGGNCSSKNKKKKKKLTGPVIAKKTMFITGMHCEQCVQSVTDSLNR"
     gene            complement(325..2331)
                     /gene="feoB"
                     /locus_tag="ACZPD3_08575"
     CDS             complement(325..2331)
                     /gene="feoB"
                     /locus_tag="ACZPD3_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HIR1416075.1"
                     /translation="MRIAFAGNPNSGKTTMYNALTGRNERVGNWAGVTVERKESPIKK
                     GYYDGTEELVAVDLPGAYSMSPFTSEESITSGYVKNENPDVIINIVDATNLSRSLFFT
                     TQLLELGIPVVVALNKSDIVNKKQTKIDEKILSEKLGCPVIKTISTSSGHEGLKETVN
                     AAAALRGKGQKAPYVQGDIDFKDKTAVEAADRKRFEFVNGIVKQVEKRKVFTKDRNVQ
                     DKIDGIITHKIIGIPIFAAVIFLVFYISQTTLGTWIADWLVAWIETFQGWVGGLMENA
                     NPLLYALLVDGIIGGVGAVVGFLPLVMVMYFLIALLEDCGYMARATVVLDPIFKRVGL
                     SGKSVIPMVIGTGCAIPGVMACRTIRNERERRTTAMLTPFMPCGAKIPVIALFAGAFF
                     ADAWWVSATMYLVGILLVLLGALLVKKITGQKYRKSFFIIELPEYKFPSLKRACGSML
                     ERGKAYIVKAGTIILVCNTVVQIMQSFNWQFQLVEEGAEGTSILASIAHPFAILFIPL
                     GFGVWQLAAAAVTGFIAKENVVGTLAVVYGITNLIDTDELALVGSGNEVATVMGLTKV
                     AALAYLMFNLYTPPCFAALGAMNSEMKSGKWLLGGICLQLATGYTVAFGVYQIGTLIT
                     TGSFGTAFIPGLIAVIVFALIILWRIRKSDKEFASEYSLHSVKS"
     gene            complement(2434..>2563)
                     /locus_tag="ACZPD3_08580"
     CDS             complement(2434..>2563)
                     /locus_tag="ACZPD3_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HIR1416076.1"
                     /translation="FSLGCYSGEPITVVSHLKGGCVVSIKDGRYNIDTDLAKAISI"
BASE COUNT          721 a          652 c          434 g          756 t
ORIGIN      
        1 cgattcaagc tatctgtcac actttgtaca cactgttcac aatgcattcc tgtaataaac
       61 atggttttct ttgcgatgac cggacccgtc aatttctttt ttttcttttt atttttgctg
      121 ctgcaatttc ctcccgctgg acatccaaca catttcactc cgcttctttt tgccttaata
      181 agatatgcag ctgcactccc tactaagatt acaataacaa gaactgctat catattttcc
      241 atagagcatt tcacatcctt ttctaataaa aatggtggac aaagtacttc ttctctgtcc
      301 accgtctgct ataactttta tcctttatga ttttacagaa tgcaggctgt attctgaagc
      361 aaactcttta tctgacttac ggattctcca gagaataatc aaagcaaata caataaccgc
      421 gataagtccc ggaataaatg cggtgccaaa agatcctgtt gtgatcaatg taccaatctg
      481 atatacacca aaagctacgg tataccccgt tgcaagttgg agacagattc ctccaagcag
      541 ccattttcca ctcttcattt ctgaattcat cgctccaaga gctgcaaaac aaggtggtgt
      601 gtaaaggtta aacatcaaat aagcaagagc tgctaccttt gtcagtccca taacagttgc
      661 tacttcattc ccacttccaa ccaacgccag ttcatctgta tctattaagt ttgtgatacc
      721 gtaaactact gcaagcgtcc caactacgtt ttcttttgcg ataaaacctg tcactgccgc
      781 tgctgcaagc tgccacactc caaatccaag cggtatgaac aaaatcgcaa acggatgggc
      841 aatggatgcc aaaatagatg tcccttctgc accttcttct acaagctgga actgccagtt
      901 aaagctctgc atgatctgta ccaccgtatt gcataccaga atgatcgttc ctgcttttac
      961 gatatatgct tttcctcgct ccagcataga gccgcatgcc cttttcaagc ttggaaactt
     1021 atattccgga agttcaataa taaagaaaga ttttctgtat ttttgtcctg taatcttttt
     1081 cacaagtaaa gctccaagca aaactaagag aatacccacc agatacatag ttgctgaaac
     1141 ccaccatgcg tccgcaaaga atgcacctgc aaaaagagca atcactggga ttttagctcc
     1201 acatggcata aatggtgtaa gcatcgctgt ggtacgacgt tctctttcgt tacggattgt
     1261 acggcaagcc ataacgcccg gaatcgcaca tcctgtacca atgaccatag gaatgacaga
     1321 tttaccggaa agccctactc tcttaaaaat cggatccaga actactgtag ctcttgccat
     1381 gtatccacaa tcttccaata atgcaattaa gaagtacatg accataacaa gcggaaggaa
     1441 accgacaact gcaccaacgc caccgatgat accatctaca aggagtgcat acagaagcgg
     1501 gtttgcattt tccatcagtc cgccgaccca tccctggaaa gtctcgatcc atgccaccag
     1561 ccagtctgcg atccaggtac cgagcgttgt ctgggatatg tagaacacaa ggaagataac
     1621 tgctgcgaaa atcggtatac cgataatctt atgtgtgatg ataccgtcaa tcttatcctg
     1681 tacgtttcta tcctttgtaa aaacttttct cttctctacc tgctttacaa ttccattcac
     1741 aaactcaaaa cgctttctgt cagccgcttc tactgccgtt ttatctttga aatcaatatc
     1801 cccctgcaca taaggtgctt tctgaccttt tcctcttaat gcagccgcag catttacagt
     1861 ctctttgagt ccttcatgtc cggaagaagt ggaaatcgtc ttgattaccg gacagcccag
     1921 cttttcagac agaatcttct cgtcaatttt tgtctgtttc ttattaacga tatcgctctt
     1981 attcagtgcg acaactactg gaatcccaag ttccagaagc tgtgtggtaa aaaacaggct
     2041 tctgctcaga ttcgtagcat ccacgatatt gatgatcaca tccggattct cattcttgac
     2101 gtatccgctg gtaatgcttt cttccgatgt aaaaggagac atagaataag cgcccggcaa
     2161 atctaccgca actaactcct ccgtcccgtc ataataacct ttcttgatcg gactttcttt
     2221 tctttcgacc gtaacgcctg cccagttccc gacacgttca ttcctgccgg taagtgcatt
     2281 gtacatggtc gtctttccac tgttgggatt tcctgcaaaa gcaatcctca taattctcct
     2341 ccttttcgac gtctttaatt atacgcatct tatctttatt agtatgcact aacctttgtg
     2401 caaaaaaaaa tagtggaaac tactattttt ttattatatt gaaatggctt tcgccagatc
     2461 agtatcaata ttataacgcc catccttaat ggagactaca cagccaccct tcagatgaga
     2521 aaccactgta attggttcac cactgtaaca gccaagagaa aac
//
LOCUS       DBMDXG010000326         2546 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8840_length_2546_cov_1.692025, whole genome shotgun sequence.
ACCESSION   DBMDXG010000326 DBMDXG010000000
VERSION     DBMDXG010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8840_length_2546_cov_1.692025"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="ACZPD3_08585"
     CDS             complement(<1..386)
                     /locus_tag="ACZPD3_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR1416077.1"
                     /translation="MMTEVIIGSRESKLAVLQSEMVKSYIEQKNREKNAGSEITVNIL
                     TMKTTGDIILDRTLDKVGGKGLFVKELDRALLDGKSNLSVHSLKDMPMEVPEELPLLA
                     FSKREDPRDVLVLPEGVAELDPDKPLG"
     gene            complement(376..873)
                     /locus_tag="ACZPD3_08590"
     CDS             complement(376..873)
                     /locus_tag="ACZPD3_08590"
                     /EC_number="1.3.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 dehydrogenase/sirohydrochlorin
                     ferrochelatase family protein"
                     /protein_id="HIR1416078.1"
                     /translation="MKNKPYFPMFIDLSDKNIVVVGGGNIATRRVKTLLSFTRNIRVI
                     APKVTMEMMELGKAGYVELITRPVKRTDFAMAYMVIAATNDRKLNDEIHRICRQEGVY
                     VNVASDREQCDFYFPGIYMEEGLVVGITASGLDHKKARRIREEIQNALERVAQEDDNE
                     EENDD"
     gene            complement(863..2092)
                     /gene="hemA"
                     /locus_tag="ACZPD3_08595"
     CDS             complement(863..2092)
                     /gene="hemA"
                     /locus_tag="ACZPD3_08595"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR1416079.1"
                     /translation="MSISMIGIDHNMAPVDIRAKFAFTKKNAGEAMEKIKNQNGIYGC
                     VILSTCNRLEVWASVDDEVDVCLYDCLCRIKGITEDSYRQYFVERKDQEAVEHLFYLT
                     SGLKSQIIGEDQILTQVKDALNLARENFAADGVLEVLFRMAATAGKRIKTEVPFSHGN
                     PSVIHQAIGFLAEKGYSLRDKTCMVIGNGEMGKVAAQALQEAGADVTVTIRQYRSGVV
                     NIPLGCKRINYGDRMEYMPQCDLVVSATASPNFTLKEELFQGIRTKDDLILIDLAVPR
                     DIEPSVGKIQGVTLYDMDSFRIEEVPAELQENLEAAGAIVREQMDEFFQWLDGRDLIP
                     RIQDIKDEAVNDLNLRIAKILKKTPMEEDDRQNLVHAVDTAAGKVVNKLIFGLRDSLN
                     QEIFLECVAGLEKIYEE"
BASE COUNT          651 a          689 c          425 g          781 t
ORIGIN      
        1 cccagaggtt tatccggatc cagctccgcc actccctcag gaagcaccag tacatctctt
       61 ggatcctcac gctttgaaaa ggccagaagc ggaagctctt ccggcacctc cataggcata
      121 tccttaagac tgtgtacaga cagattgctt ttaccatcca gcaatgccct gtccagttct
      181 tttacaaaca atccttttcc gcccacctta tccaaagtac ggtccagaat aatatctcct
      241 gttgttttca tagtcaggat atttactgtt atttcagagc cggcattttt ttcacggttt
      301 ttctgctcta tgtaactttt taccatctca ctctggagaa ctgccagctt actctctctg
      361 cttccaatga tcacctcagt catcattctc ctcctcatta tcatcttcct gcgcaaccct
      421 ctccagggca ttctggatct cttctcttat cctgcgcgct tttttatggt ccagaccact
      481 ggctgtaatt cccactacca gtccctcttc catataaatt cccgggaaat aaaaatcgca
      541 ctgctccctg tcactggcta cattaacata aacgccttcc tgcctgcaga tcctgtggat
      601 ctcatcattt aactttctgt cattggtagc tgcgatcacc atatatgcca tcgcaaaatc
      661 tgtacgcttc accggacgtg tgatcagctc tacatagccg gctttgccaa gctccatcat
      721 ctccatggtg accttcggag caatgactct gatatttctg gtgaatgata aaagggtttt
      781 cactcgacga gttgcaatgt ttccgccgcc tacaaccacg atattcttat ctgataagtc
      841 tataaacatt ggaaaatacg gcttattctt catatatttt ctccagtcct gccacacact
      901 caaggaaaat ctcctgattc agactgtcac gcagaccaaa gatcagttta tttactactt
      961 ttcctgctgc cgtatccact gcatggacca gattctgcct gtcatcctct tccataggtg
     1021 ttttctttaa tatctttgca atccttaaat tcaggtcatt gactgcctca tctttaatat
     1081 cctgaattct tgggatcagg tctcttccat ccagccattg gaaaaattca tccatctgct
     1141 ctcttacgat cgcgcctgct gcctccagat tttcctgaag ctctgcagga acttcctcta
     1201 tacggaagct gtccatgtca taaagtgtga ccccctggat ttttccaaca gatggctcta
     1261 tatctctggg aactgccaga tcgatcagga tcaggtcatc ctttgtcctg attccctgga
     1321 acaattcttc ttttaatgta aaattcgggc ttgcagttgc acttactaca agatcacact
     1381 gtggcatata ctccattctg tcaccgtaat taatacgctt gcagcccagc ggaatattca
     1441 caactccgct tctgtactga cggatggtca ctgttacatc tgcacctgcc tcctgcaagg
     1501 cctgagcagc caccttgccc atctcaccgt taccgatgac catacaggtc ttatccctga
     1561 gagaataccc cttctctgcc agaaatccga tggcctgatg gatcactgac ggatttccat
     1621 gggaaaaagg aacctctgtc ttaattctct ttcctgctgt ggcagccatt ctgaacagaa
     1681 cctccaaaac accatctgcc gcaaaattct ctcttgccag attcagggca tccttcacct
     1741 gtgtaaggat ctgatcctcc cctatgatct gtgatttcag accactggta agataaaaaa
     1801 gatgctcaac tgcctcctga tccttacgct ctacaaaata ttgtctgtaa gaatcctcag
     1861 taattccctt tatcctgcag aggcagtcat aaagacagac atccacctca tcatccacac
     1921 ttgcccagac ctccaggcgg ttacaggtgg acaggatcac gcatccatat attccatttt
     1981 gattctttat tttttccatt gcttcaccgg catttttctt tgtaaatgca aatttcgcac
     2041 gaatatccac cggtgccata ttatggtcaa ttccaatcat ggaaatactc ataacttcta
     2101 actctccttc gtatttcacc attcctcatt catttcatat caaatcaaat atcccactgg
     2161 caacagggta tttgatttga taagaaatcc ggacagctaa aaagcactgt cagagatatc
     2221 ataatcattt cctgttaaga tgtcaattgt aaacacggaa aaaactcagc attagagtaa
     2281 aggatgttac tgttcacata ccactatccc gagacataca agccaaaatt ccattgccgc
     2341 aggcaatctg gaatgaattt tggctaagtt actgtgaacg gagtgaacag taactaaagg
     2401 ataacgtgac aagacctcat taccgttcgt tctgcatccg gtaatgaggt cagaatattc
     2461 tgtgtttact gtttatctat tctcatcaaa actttacatt tctttctgta acaggcgatt
     2521 ccatatttta tgattgtatg aattcc
//
LOCUS       DBMDXG010000327         2545 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8846_length_2545_cov_1.152619, whole genome shotgun sequence.
ACCESSION   DBMDXG010000327 DBMDXG010000000
VERSION     DBMDXG010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8846_length_2545_cov_1.152619"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="ACZPD3_08600"
     CDS             complement(<1..183)
                     /locus_tag="ACZPD3_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HIR1416080.1"
                     /translation="MRMMKNNRNETIMVIGIDHGYGNMKTATRCFPSGVARYDEEPIF
                     QNNLLVYNGMYYQIGEE"
     gene            complement(378..>1878)
                     /locus_tag="ACZPD3_08605"
     CDS             complement(378..>1878)
                     /locus_tag="ACZPD3_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HIR1416081.1"
                     /translation="KEYLGKLDNQKEVKKLLEQYILRFERNPKEEPELSRFHLFDAKG
                     NVKGVRDMEIVDYLVENVQFFVVGITPYYYEHGVFLEDHDGVRMKYRIQKLIYRDQVQ
                     SGVIKRIYNLLITQPKVHREAYELNKQPVRWINFKNGYYDPVTGEMLEHNPDYLTINQ
                     IPFPYYPEDREQVLQGGENIKKYLASSLPNKEEQKTFWEYFGYCMTQDTQFQKFLTLK
                     GNGGTGKSVAVSLIQHVVGITNMSSISLQDLNKRFYATGMYGKLLNACADIPCKAMEN
                     TDVLKKAVGEDTLIYEKKGQDAIHFHSYAKLLFSTNEMPQNLEDKSDAFYRRLLILDM
                     NRVVKSGEKDLHLEEKVQAESDYAIHMAMLALKKLYEQGKFTESEHSKECVREVQRSS
                     DSICAFIDETLVRAKGKRLKRSEVFNMYEEYCKENGRQGHGKSNFFRNMTDKGFLLKQ
                     YNGEFYYQDIAVKEEDFCPVDPEERIPFEETDTDYKQLQLNMNQGIKGI"
     assembly_gap    1879..1888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1889..2101)
                     /locus_tag="ACZPD3_08610"
     CDS             complement(<1889..2101)
                     /locus_tag="ACZPD3_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HIR1416082.1"
                     /translation="MISLNKKFLKHYMETNPEGTAQTYIFLVDNQDIALNIVMSGYQA
                     LCLVQEDDGYYFSADSFIEEMRTIQFT"
     gene            complement(2220..>2545)
                     /locus_tag="ACZPD3_08615"
     CDS             complement(2220..>2545)
                     /locus_tag="ACZPD3_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIR1416083.1"
                     /translation="NGYLIPNLTYKSGEQMEQLGKYGFLRRDYLKNHRNSTYQVMLLQ
                     DTIGEHLQEVDKAAREREEIILKQLEEKDPLPDKEKDQMAWVRAANQHRAIAEEIILK
                     ELIYV"
BASE COUNT          676 a          607 c          379 g          873 t
ORIGIN      
        1 ttcctctccg atctggtaat acattccatt gtaaacaaga agattattct gaaaaattgg
       61 ctcctcgtca tatctggcta caccggacgg aaagcatctg gtagctgttt tcatgttgcc
      121 atagccgtga tcaatcccaa tcaccataat tgtttcattt ctgttatttt tcatcattct
      181 cataatcata cctcatactt tcttaatgtt tatttttgac tttctcccaa attcgggaaa
      241 acttatattt gtatcagcta aaacgttgat acatgaaaaa gggcagtcag tgtaaaaaaa
      301 gcaaaatttt atgtactctg gcagctatct ttgttatttc tcctgctccg gtaacatttc
      361 tttttgctta ttttgcccta aatgccctta attccctgat tcatatttaa ctgtaactgt
      421 ttataatctg tatctgtttc ttcaaaaggt attctttctt ctggatctac aggacaaaaa
      481 tcttcttcct taactgcaat atcctgataa tagaactcac cattgtactg cttcaagaga
      541 aatcctttat ccgtcatatt cctgaaaaag ttggatttac catgcccctg tctgccgttc
      601 tctttgcaat attcctcgta catattaaac acttcacttc gtttcagacg ttttccttta
      661 gcacgcacca atgtctcatc aataaaagca caaatactgt cagatgaccg ctgtacctct
      721 cttacacatt ctttgctgtg ttcactctcc gtaaactttc cctgttcata caatttcttc
      781 agtgcaagca tcgccatatg aattgcataa tcagattctg cttgtacctt ttcctctaag
      841 tgcagatctt tttcaccact ttttactacc cggttcatat cgagaatcag tagtctacga
      901 taaaaagcat ctgacttgtc ctcaagattt tgtggcattt cattggtaga aaaaagcagt
      961 tttgcataag agtggaaatg aattgcatcc tgtccttttt tctcataaat cagtgtatcc
     1021 tcaccgactg ctttctttaa tacatctgtg ttctccattg ctttgcaggg aatatccgca
     1081 caggcattta acagctttcc gtacattcca gtcgcataga atcgtttgtt caaatcctgt
     1141 aaagaaatgc tggacatatt ggtgattcca actacatgct ggatcagcga tactgccaca
     1201 gactttccgg ttccaccatt tcctttcagt gtaaggaatt tctgaaactg cgtatcctgt
     1261 gtcatgcagt atccaaaata ctcccagaat gtcttctgtt cttctttgtt gggaagagac
     1321 gatgcaagat actttttgat attttctcca ccctgcaaca cctgctccct gtcctccgga
     1381 taatagggaa atggtatctg attgattgtc agataatctg gattgtgttc cagcatttca
     1441 cccgtaaccg gatcataata tccattttta aagttaatcc accgcacagg ctgtttattt
     1501 agttcatatg cctctctgtg gacttttggc tgtgtgatca gcagattata gattcttttg
     1561 atcacgccac tctgtacctg atctcgatag atcagcttct gaatacggta tttcattctt
     1621 accccatcat ggtcttccag gaaaacgccg tgttcataat aataaggtgt gattcccacg
     1681 acaaaaaact gaacattctc caccagataa tctacaatct ccatatcccg gacacctttc
     1741 acatttcctt ttgcatcaaa taagtgaaaa cggctcagtt ccggttcttc tttcggattt
     1801 cgctcaaacc gaagaatata ctgttccagt aatttcttaa cttctttctg attatccagt
     1861 ttgccaagat actctttcnn nnnnnnnnag taaactgaat agttctcatt tcctctataa
     1921 aagaatctgc actgaaataa tacccatcat cttcctgcac taaacaaagt gcctgataac
     1981 ctgacattac aatattcaac gctatatcct gattgtccac caaaaagata taggtctgtg
     2041 ctgtcccttc tggattggtt tccatataat gcttcaaaaa cttcttgttc aaacttatca
     2101 cttcctttcc aggcaaaaaa agacgcaagc cacatttcta tgactcacgc ctttctctgc
     2161 acttatttac tttacagttc caattcgcta cgcactgcgt atccttttag aacttgcatt
     2221 taaacataaa tcaattcctt gagaataatc tcttccgcaa tcgctctgtg ctgattagca
     2281 gctcttaccc atgccatctg atctttctcc ttatccggca atggatcttt ttcttccaac
     2341 tgtttcagaa taatttcttc ccgttctctg gcagccttat ccacttcctg cagatgctca
     2401 ccaatcgtat cctgcaaaag catcacctga taggttgaat ttcgatggtt cttcagataa
     2461 tctctacgaa gaaaaccata cttgccaagt tgctccatct gctcaccgga tttataggtg
     2521 agatttggaa tcagatatcc attta
//
LOCUS       DBMDXG010000328         2543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8853_length_2543_cov_1.580672, whole genome shotgun sequence.
ACCESSION   DBMDXG010000328 DBMDXG010000000
VERSION     DBMDXG010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_2543_cov_1.580672"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="ACZPD3_08620"
     CDS             complement(<1..754)
                     /locus_tag="ACZPD3_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR1416084.1"
                     /translation="MRKNRQTAKYHIKSYIIAGSLAGAISMALALPVPVMAAQQSLAS
                     VTGQAQIQPMGDGSGKYMMKSDGFYCLDVNGAGSTQAEIHYFQDYEIDGTVFDGYYYH
                     DADGKFKACSPHMEHLKGVAVFGDKTDEEADTQNAQEAEKFDGYYFVNNLGRLSAAPQ
                     VRYIDNLAIDGITLNGYYYFDENGRLVTEPGIHSLEMDCYEMNFDGSYYFGGTNGALL
                     QESTVTDDGFIVDDTGKIVNMDDLGMDNLKPQL"
     gene            complement(880..>2543)
                     /locus_tag="ACZPD3_08625"
     CDS             complement(880..>2543)
                     /locus_tag="ACZPD3_08625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR1416085.1"
                     /translation="RAVGAGLGENIFYLTAKTITRTVAEEAFSILKEHGLKFKVITIT
                     AKEKLCFCDKTECNPENCLWARGHLDRVNDAVFELWTTQDSYDRDTLLEYAKKWQVCP
                     FEMCLDLAVWVDAVICDYNYVFDPNVYLKRFFGEGTSGEYIFLIDEAHNLVDRGREMY
                     SAHVDRADVLEAKRLAGDYSKGLVRALEKVNRQLRTLEKECTEYEILPNPGAVSLGML
                     QVMGEMDKLLEKLHGKELPEQLLEFYFCVRDFLNIDELLDENYVVYTEMGEGGKVILR
                     LFCVNPAANIHRCLEKGKSAVFFSATLLPMDYYRALLSTRKDDYGIYVTSPFRQENRC
                     ILTGRDVSSRYTRRGYEEYHRIASYIARTVWTRKGNYMVFFPSYKFMEDVLEVYENEF
                     SAEWVRCISQTSGMNEREKEEFLEEFSASEGTLVGFCVMGGIFSEGIDLMGEKLIGAI
                     IIGTGLPQIGTEREILRQYYDKKGVNGFDYAYRYPGMNKVLQSAGRVIRTQEDTGIIL
                     LLDERFAGKDYRNLFPAEWSDRGNCTLNTVEEQLGSFWNRIREKN"
BASE COUNT          661 a          719 c          407 g          756 t
ORIGIN      
        1 caagctgagg cttcagatta tccatcccaa gatcatccat attcacgatc tttcccgtat
       61 catccacaat aaaaccatca tccgtcactg tgctttcctg aagcagggcg ccatttgtac
      121 cgccaaaata atagcttccg tcaaaattca tttcataaca gtccatctca agtgaatgga
      181 tacctggctc ggttaccagt ctgccatttt catcaaaata ataatatccg ttcagagtaa
      241 tcccgtctat tgccagattg tcaatatacc ttacctgcgg tgctgctgac agacgcccca
      301 gattgttcac aaagtaatat ccgtcaaatt tctctgcctc ctgggcattc tgtgtatcag
      361 cttcctcatc tgtcttatcc ccaaacactg caacaccttt cagatgctcc atatgaggac
      421 tgcaggcttt gaatttccca tctgcatcat ggtaataata tccgtcaaat acagttccgt
      481 ctatctcata atcctgaaaa taatggatct ccgcctgggt acttccggcg ccattcacat
      541 ccagacagta aaatccgtca ctcttcatca tatatttgcc acttccgtct cccatgggct
      601 gaatctgtgc ctgcccggtg acagatgcaa ggctctgctg tgcagccata acaggaaccg
      661 gcaaagccag tgccatggaa atggctccgg ccagacttcc ggctatgata taacttttaa
      721 tatgatattt cgctgtttgt ctattttttc tcatgcaaaa ctccttttac ggtttctttt
      781 cacatcccat tcgcagtaag caaagtatat attgttttat tcaggatagc aagattttta
      841 tcttttttcc agcaaagacc agaatctcag tatcagtgtt cagttctttt ctcttatcct
      901 gttccagaag cttcccagct gttcttccac agtatttaaa gtacagtttc ccctgtcaga
      961 ccactcagca ggaaacagat tcctgtaatc tttccctgca aatctctcgt ccagcagaag
     1021 tatgatccct gtgtcctcct gggttctgat cacacgcccg gctgactgca gcaccttatt
     1081 catccccgga tatctgtatg cataatcaaa tccattcaca ccctttttat cataatactg
     1141 cctgaggatc tctctttctg ttccaatctg aggaagacca gtccctatga tgattgctcc
     1201 gatcagcttc tctcccatca gatcaatccc ctctgaaaaa atcccgccca tcacgcaaaa
     1261 tcctaccagc gttccctctg atgcagaaaa ttcctccaga aattcttctt tctccctttc
     1321 attcattccc gatgtctgcg aaatacaacg aacccattcc gctgaaaact cattctcata
     1381 cacctccaga acatcttcca taaatttata agaaggaaag aataccatat aatttccttt
     1441 tcttgtccat acagttctgg caatgtaaga tgcgatcctg tgatattcct catatcctct
     1501 tctggtatac ctgctgctca catcccgtcc tgtgagaatg cacctgttct cctgcctgaa
     1561 aggagaagtc acataaatcc catagtcatc ctttctggtg cttaaaagtg cacggtaata
     1621 atccatggga agcagagtcg cagaaaagaa caccgcactt tttccctttt ccagacatct
     1681 atgaatattt gctgcaggat ttacacagaa aagccgcaaa atcacctttc ccccttctcc
     1741 catttctgta taaaccacat aattttcatc cagaagttca tcaatgttca ggaaatctct
     1801 cacacagaag taaaattcca gcagctgctc cggaagttcc tttccatgaa gtttctccag
     1861 gagcttatcc atctccccca tcacctgaag cattcccaga gaaactgctc cgggatttgg
     1921 caggatttcg tactctgtac actccttttc cagagttcga agctgtctgt tcactttttc
     1981 cagtgctcgc accagacctt tgctgtaatc cccggccagt ctcttcgcct ccagtacatc
     2041 cgctctatcc acatgagcac tgtacatttc ccttcccctg tccaccagat tgtgagcttc
     2101 atcgatcaga aaaatgtatt ctccggaagt accctccccg aaaaatcttt tcagataaac
     2161 attgggatca aatacataat tataatcaca gatcacagcg tccacccaga ctgccagatc
     2221 caggcacatt tcaaagggac acacctgcca tttcttcgca tattctagca gagtatccct
     2281 gtcatagctg tcctgcgtag tccacagttc aaaaacagca tcattcactc tgtccagatg
     2341 tcctctcgcc cacagacagt tctcaggatt gcattctgtt ttatcgcaga agcacagctt
     2401 ttccttcgcg gtaatggtta tcactttaaa tttcagtcca tgctccttta atatggagaa
     2461 agcttcttca gccacagtcc tggtaatggt ctttgctgta agatagaaaa tattttcccc
     2521 cagtcctgca ccaactgctc tca
//
LOCUS       DBMDXG010000329         2543 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8855_length_2543_cov_1.174857, whole genome shotgun sequence.
ACCESSION   DBMDXG010000329 DBMDXG010000000
VERSION     DBMDXG010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8855_length_2543_cov_1.174857"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            525..>2543
                     /gene="mutS"
                     /locus_tag="ACZPD3_08630"
     CDS             525..>2543
                     /gene="mutS"
                     /locus_tag="ACZPD3_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR1416086.1"
                     /translation="MAMSPMMQEYCKTKEQYKDCILFYRLGDFYEMFFDDALLVTKEL
                     EITLTGKDCGLEERAPMCGVPYHAAETYINRLIERGHKVAICEQVEDPKKAKGLVKRE
                     VVRIVTPGTTLDAAALDETKNNYLMSIVSMEEHFGCAIADITTGDCFLTEVDKPQKLL
                     DEINKFVPAEIICNDSFYMSNIDTDDLQNRLGICVFSLDSWYFDDELCRRTLKDHFHV
                     GSLEGLGVGDYDCGIIAAGALFLYLKETQKTALSHMTTIRPYAAEKYMLIDSSSRRNL
                     ELVETLREKQKRGSLLWVLDKTKTAMGARTLRSYVEQPLIDRDEIEQRLEALEELNKN
                     GMLRDEIREYLGPVYDLERLISRISYKSANPRDLIAFASSLEMLPYIKQVLKEFKTPL
                     LQKIYEDMDSLEDVTDLIKRAIVEDPPLAQKDGGIIKEGYNEDVDKFRRSRTDGKKWL
                     SELEAKERERTGIKTMKIKYNRVFGYSLEITNTFKDLVPDNYIRKQTLTNAERYITQE
                     LKELEDLILGAEDKLYALEYELFCDVRDAVGKEVMRIQKTAKAVAALDVFASLALVAE
                     RNHFVRPKTNTTGVIDIKNGRHPVVEQMIENDMFIANDTYLDNHKKRVSIITGPNMAG
                     KSTYMRQTALIVLMAQIGSFVPAEKANIGIVDRIFTRVGASDDLASGQS"
BASE COUNT          815 a          455 c          598 g          675 t
ORIGIN      
        1 tttcaacctg tatgcattca gaaattcaac tggtgactgt tgcatataat gcttaaaaat
       61 ctggcagcac ttactaggaa acgacttctg aggaatccgg cataagctct gttctgatta
      121 aatccaaaaa actcacctcg tgtacgattt ttataaaaat caaagactat tctgatagat
      181 ttccatatat gagaatacta taattatagc atctgcaaaa gtaaaaaatc aatcggaaac
      241 aatgtagtga ttggcgcagg aagtgtggta tccaaagata tccctgataa tatgagcgca
      301 gctgccctgg aaagtaaact gatcgtgact acgctgaaat aaattataaa gggatggtga
      361 aggaggatgt ttagtaacat atagtcaaca tgatagaata aatgtttata ctctatcttg
      421 catcttctat ttattcatgt taaaatctta agatgtcaaa aatacaatat atacgagata
      481 aaaaacagca gtgagctgtt atgaagatag gagataacaa ttaaatggca atgagtccga
      541 tgatgcagga atactgcaag acaaaggaac aatataaaga ctgcattcta ttttaccgtc
      601 tgggagattt ctacgagatg ttttttgatg atgcgttgct tgtcaccaaa gagctggaaa
      661 tcacacttac aggaaaagac tgcggattag aggagagggc acccatgtgt ggtgtccctt
      721 atcatgctgc agagacttat atcaatcgtc tgatcgagag aggacataag gtagcaatct
      781 gtgagcaggt agaggatcct aaaaaagcca aaggactggt aaagcgtgag gttgtaagga
      841 tcgtaacccc aggaaccaca ctggatgctg ctgctcttga tgagacaaag aataattacc
      901 tgatgtctat tgtgtctatg gaagaacatt tcggatgcgc tattgcagac attacaacag
      961 gtgactgttt ccttacagag gtggacaaac ctcagaaact tcttgatgag atcaataaat
     1021 ttgttcctgc agagattatc tgtaatgatt ctttttatat gtctaatata gatacagatg
     1081 acttgcagaa ccgtcttggg atctgtgttt tttcattgga ttcctggtat ttcgatgacg
     1141 agttgtgccg ccgcacacta aaagatcatt ttcatgtagg ttctctggag ggattgggcg
     1201 ttggtgatta tgactgcggg atcattgctg caggagctct tttcttatat ctgaaagaaa
     1261 cccagaagac agccttgtcc catatgacaa cgatccgtcc ctatgcagca gagaaatata
     1321 tgcttatcga cagttccagc cgcagaaatc tggaacttgt ggaaaccctg agagagaaac
     1381 agaaaagagg ttctcttctt tgggtactgg ataagacaaa aacagcaatg ggagccagaa
     1441 ctctccgcag ttatgtggaa cagccactga tcgacagaga tgagattgaa caacgtctgg
     1501 aagcactgga agagttaaat aaaaacggaa tgctccggga tgagatcagg gaatatctgg
     1561 gacctgttta tgacctggaa cgactgatca gccgtatcag ctataaatct gcgaatccaa
     1621 gagatctgat cgcatttgct tcttcgctgg aaatgcttcc ttatataaaa caggttttaa
     1681 aagaatttaa aacacctctg cttcagaaaa tctatgagga tatggattcg ctggaggatg
     1741 tgacagatct gatcaagcgt gccatcgtgg aagatccgcc tcttgcacag aaggatggag
     1801 gcattattaa agagggctat aatgaagatg tagataaatt ccgccgttcc aggacagatg
     1861 gtaagaaatg gctttcagaa ctggaggcca aagagcggga gcgtacaggc atcaaaacaa
     1921 tgaagatcaa atataatcgc gtatttggtt attcactgga gatcaccaat acttttaaag
     1981 atctggtgcc ggataattat atccgtaaac agacgcttac caacgcagag cgatacatta
     2041 cccaggagtt aaaagaactg gaagacctga tccttggggc agaggataag ctgtatgctc
     2101 tggaatatga actgttctgt gatgtgagag atgcagtcgg gaaagaagtt atgcggatcc
     2161 agaagactgc caaggcagtg gctgcactgg atgtgtttgc ttctcttgcc ctggttgcgg
     2221 aaagaaatca ttttgtacgt ccgaagacaa atacaaccgg tgtgatagat attaaaaacg
     2281 gccgtcatcc tgttgtggaa cagatgatag agaacgatat gtttattgcg aatgatacat
     2341 atctggataa ccataagaaa agggtatcca ttatcacagg tccgaacatg gcaggtaagt
     2401 ctacctacat gagacagact gccctgatcg tactgatggc acagatcgga agttttgtcc
     2461 ctgctgaaaa agcaaatatt ggaattgttg acaggatttt cacacgtgta ggtgcctctg
     2521 atgatctggc aagtggtcag agt
//
LOCUS       DBMDXG010000330         2521 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_8940_length_2521_cov_1.779339, whole genome shotgun sequence.
ACCESSION   DBMDXG010000330 DBMDXG010000000
VERSION     DBMDXG010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8940_length_2521_cov_1.779339"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="ACZPD3_08635"
     CDS             complement(<1..559)
                     /locus_tag="ACZPD3_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416087.1"
                     /translation="MRRNEQEQLEKEIKDVRDRNSKLVMQVNQSSVEAVEQAKKKQEE
                     AEKQARQAEYQIEREKNRADMEIQRARRKAKSEIADMKENQFFWDWGYLCVIFFSFLQ
                     NGAFQQDVLQLIMIPINWCKEYIAWFERLEYISDTAGEMLFKGFFSMLMITAGILGCV
                     FLVWAGVEQYKKIWDDLYKMVLISSI"
     gene            complement(495..1736)
                     /locus_tag="ACZPD3_08640"
     CDS             complement(495..1736)
                     /locus_tag="ACZPD3_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HIR1416088.1"
                     /translation="MAINKTINKRTNTHGAMRNCIEYVLRQDKTSEQLTYVTGPYCHD
                     EINYDLVYRAFLEEKKVWDKDSGRMYAHNIISWHKDEQITLEQAFEFGKEFAEKWFQG
                     FQTLVAVHKDKDHIHCHLVTNSVSYEDGRKLHNTKKDLERMKQLTNQMCRERGLTVVE
                     KGKHFDGSQIEKGEVIAWSKDKYNLFRQQVKDSFVADCAMAVLKALENCISKEKFIEK
                     MKQFGWRVNWTEKRKHITFQNQDGKKVRDSNLSKTFHLDISKEALEHEFNGNYERIRA
                     EAERTAGADEELIGYYRQVEAACEGTGRNTGESDGREGRVTGEKSEDERVYPGISEKN
                     TQSENGKTEAILRESRNARRNAEVECRNSADDRRIVRNAETQSVFTAEQRRLEEQKRL
                     DEKERARAARKRNKRRSGPEL"
     gene            complement(1685..2065)
                     /locus_tag="ACZPD3_08645"
     CDS             complement(1685..2065)
                     /locus_tag="ACZPD3_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein"
                     /protein_id="HIR1416089.1"
                     /translation="MRKRNHTVTIRMNKAEYELLQSKVKESGRTQQEVVIKAIADLKI
                     ASTEEVEELKRLNQMFADILSQLRGATTNINQIARKLHTDGEIPNDSMLYFLNKNILK
                     YRKESEKIWLLIRRLISGQIHMEQ"
BASE COUNT          568 a          645 c          421 g          887 t
ORIGIN      
        1 aaatactgga tattaaaacc attttatata ggtcatccca tatcttctta tattgttcaa
       61 ctccagccca aacaaggaat acacatccga gaatcccagc agtaatcata agcatagaga
      121 aaaacccttt aaagagcatt tctccggcag tatcgcttat atattccaac cgttcaaacc
      181 atgctatata ttctttgcac caatttatcg gtatcattat tagttgcagc acatcttgtt
      241 gaaatgctcc attttgaaga aacgaaaaga aaattacaca aagatatccc cagtcccaaa
      301 agaactgatt ttctttcata tcagcaattt ccgattttgc ttttctcctg gctctttgta
      361 tctccatatc tgcccggttc ttttcccttt caatctggta ctctgcctgc cttgcctgtt
      421 tttctgcctc ttcctgtttc ttttttgcct gttcaaccgc ttccaccgat gattggttca
      481 cctgcattac taatttagag ttccggtccc gaacgtcttt tatttctttt tcgagctgct
      541 cttgctcgtt ccttctcatc aagccgtttc tgttcctcaa gtcgtcgttg ctctgctgta
      601 aagactgatt gagtttccgc atttcggaca attcttctgt catctgctga atttcggcat
      661 tcgacttctg catttcttct tgcatttcgt gattctcgaa gaatagcctc tgtttttcca
      721 ttttcagact gtgtgttctt ttctgaaatt ccaggataaa ctcgttcatc ttctgatttc
      781 tcacctgtaa ctcgtccctc tcttccgtca ctttctccag tgtttctgcc tgttccttca
      841 caagctgctt ccacctgtcg gtagtaccca atgagttctt cgtctgctcc tgctgttcgc
      901 tctgcttcgg ctcgtatcct ttcgtaattt ccattaaatt catgctccaa cgcctcctta
      961 ctgatatcca gatgaaacgt tttggacaga ttgctgtcac gcactttctt cccatcctga
     1021 ttctggaatg tgatatgctt tcgcttttcc gtccagttca ccctccatcc gaactgtttc
     1081 attttttcta taaacttttc tttgctgata caattttcca gtgcctttaa taccgccatt
     1141 gcacagtccg ctacaaaact gtcctttacc tgttgacgga acaggttata tttatctttg
     1201 ctccatgcaa tgacttcccc tttttcaatc tggcttccat caaaatgctt tcctttctcg
     1261 acaactgtca gtccacgttc tcggcacatc tgattggtaa gctgtttcat acgttccaga
     1321 tcctttttgg tgttatgcag ttttctccca tcttcataac tcacagaatt tgtcaccaga
     1381 tgacagtgaa tatggtcttt atccttatgg actgccacta aggtctgaaa tccctgaaac
     1441 catttttctg cgaattcttt tccaaattca aatgcctgtt ccagagtaat ctgctcgtcc
     1501 ttatgccagg aaataatgtt gtgagcgtac attctcccag aatctttatc ccacaccttc
     1561 ttttcctcta aaaatgctct gtacaccaga tcataattga tctcatcatg gcagtacggt
     1621 ccagtcacat aggtaagttg ttcactagtc ttatcctgcc gcagcacata ttcgatgcag
     1681 tttctcattg ctccatgtgt atttgtccgc ttattaatcg tcttattaat agccatatct
     1741 tttcactctc cttcctgtac ttaagaatat ttttattaag gaaatacagc atactgtcat
     1801 tcggaatttc tccatctgta tgcagctttc ttgcaatctg attgatattt gtagtagctc
     1861 cacgaagctg actgagaata tctgcaaaca tctggttcaa tcttttcagt tcttccactt
     1921 cctctgtaga agctatcttc aagtctgcaa ttgcttttat cacaacttcc tgctgtgttc
     1981 ttccggattc ctttactttg ctttgaagta gctcatattc cgctttattc atccgtatag
     2041 ttactgtatg atttcgtttt ctcataggct tttatttcct ttctctactt gatttctgct
     2101 ctatatttac aaccatagcg ttatgtctaa attaacaggg gattccataa ggggatgtgt
     2161 aacgttcccc tttggtagat gcaagacaac gtcttagcca atccgtggca atctgagaag
     2221 aagtatccag tggatatttc taacacagaa ttgtcaccat tggcgtgccg gggttccaag
     2281 gggcagacgc ccatggcaag tttaggaaca gcccaaaggc tggctccggt gcagagtggc
     2341 ttgccacttt gcgaaacttg cctgtcatct ccccaccact gaaccgacac ccaccaagcc
     2401 atattttcca ccctacgtta tcagatcaaa atggagatga cggtggtttt gtggtgcatg
     2461 gccagcctga tctgctggga gagctatatg cccctggccc gggtcgccct gtacgagaag
     2521 g
//
LOCUS       DBMDXG010000331         2501 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9003_length_2501_cov_1.653750, whole genome shotgun sequence.
ACCESSION   DBMDXG010000331 DBMDXG010000000
VERSION     DBMDXG010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9003_length_2501_cov_1.653750"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="ACZPD3_08650"
     CDS             complement(<1..610)
                     /locus_tag="ACZPD3_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HIR1416090.1"
                     /translation="MDNYEKQVYTGRELFLKYNQDKLIEKYGLKHDEEYLYLKYIETE
                     YRINRRNGAIEYATGEEWTDCREYTVVMTIYDFLCCSGQEILPPLTGQWQPVGRFVTA
                     GSSPSTDPFVEKYARAFSGKVEEVKQACICLGGKQTKRLAGADLTFEMPVLPEFSVLF
                     QFWDGDEEFPPKILLLWDKVSLSYLHFETTYYLQGDLLKAILQ"
     gene            complement(687..1619)
                     /locus_tag="ACZPD3_08655"
     CDS             complement(687..1619)
                     /locus_tag="ACZPD3_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR1416091.1"
                     /translation="MMFLQVIILLAGFLFLVKGADWFVEGAASIAKKLGIPQLIIGLT
                     IVAMGTSMPEAAVSITAAINKNAGITIGNVVGSNILNIFIILGITAVITNVAIQKSTL
                     LYEIPFMTVITIILLIFGITGSEVTFIEGVIFWILFLIYLGYLFVMAKKGNDQEEAEA
                     KDNPVWKCMLLMVIGGILVVKGSDFAVSGATEIARYFGMSERFIGLTIVALGTSLPEL
                     VTSVTAARRGNTGIAIGNIVGSNIFNILFVIGTTALICTVPFESKFIIDTVIAVLCGV
                     ILWIGTFRHKELRKPCGVVMLLCYVAYFLYLCLV"
     gene            complement(2077..>2501)
                     /locus_tag="ACZPD3_08660"
     CDS             complement(2077..>2501)
                     /locus_tag="ACZPD3_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416092.1"
                     /translation="LLNGHTEIFGAEIGTLIYGAGKGIIRSFQDFDLCAEPYMKHPKN
                     TIYYFGDMDYEGIGIYENLAEKFRSRWKIIPFVPAYQAMLGKAEQIIELPETKEHQNR
                     NISTQFFSCFDEIMVKKMEAVLDKDRYIPQEILNTADF"
BASE COUNT          764 a          600 c          385 g          752 t
ORIGIN      
        1 tctgcagtat agccttcaga agatccccct gaagataata ggtcgtctca aaatgaagat
       61 atgataacga tactttatcc cacaacaata agatctttgg tgggaattct tcatccccat
      121 cccagaactg aaacaacaca gagaactccg gaagaacagg catttcaaat gtcagatctg
      181 ccccagccaa acgtttcgtc tgctttcctc ccagacagat acaggcctgc tttacctctt
      241 ccactttgcc ggaaaatgct cttgcatact tttccacaaa gggatctgtt gacggactgc
      301 tcccggcagt tacaaacctg cccacaggct gccactgccc ggtaagagga ggcagtatct
      361 cctgcccgga acagcagaga aaatcataga ttgtcataac aactgtatat tctctgcagt
      421 ccgtccattc ttcgcctgtg gcatattcaa tagcaccgtt tctgcgattg atccgatatt
      481 ctgtttcaat gtatttcaaa tacagatatt cttcatcatg tttcaaacca tatttctcta
      541 tcagcttatc ctgattatat tttaaaaaca actctcttcc tgtatatacc tgtttttcat
      601 aattatccat agtatacttt attccatttc ttattttaat cgaatcaagt gagtcccctg
      661 cggaggttgc gaaaagcaat tattcgttat accagacata aatacaggaa atatgcgaca
      721 taacacaaca gcataaccac accacagggt tttcttaatt ctttatgtct gaatgtacca
      781 atccagagta ttactccgca cagaactgca attacagtat caataataaa tttactctca
      841 aacggcacag tacaaatgag tgctgttgta ccgataacaa acaaaatatt aaaaatattg
      901 cttccgacta tatttccaat ggcaattcct gtatttcctc ttctggctgc tgttactgat
      961 gtgacaagtt ccggaagaga tgttcccagt gcaacaattg tcagcccgat aaatctctca
     1021 ctcatgccaa aataacgggc aatctccgtt gctccgctga cagcaaaatc actgcccttt
     1081 acaaccagaa ttccaccgat taccatcagg agcatacatt tccataccgg attatccttt
     1141 gcttctgctt cttcctgatc gttccctttc tttgccatca caaacagata tcccagatat
     1201 atcagaaaca gtatccagaa aatcacacct tcaataaaag tcacctctga acctgtgata
     1261 ccaaaaatca gcaaaataat tgttatcact gtcataaacg gaatttcgta aagaagcgtg
     1321 gatttttgaa ttgctacatt cgtaattact gctgtgattc ccaggatgat gaaaatattc
     1381 aggatattac ttccgactac atttccaatg gtaatacctg cattcttatt tattgcagca
     1441 gtaatactga ctgctgcctc cggcatgctg gttcccattg ctacaatggt taatccaatg
     1501 atcaactgtg gaattcccag ctttttcgca atactggctg caccctccac aaaccagtct
     1561 gcacctttca ccagaaacaa aaaacctgcc aacaaaataa taacctgtaa aaacatcatt
     1621 tctacctctc tttcttttat tacgtactca caaaatcaaa tataaaaaga ttccgggaat
     1681 acatttattt tgcagtaggt ataaaaaaaa gactcctact gcattcacag taaaagtctc
     1741 gcaatttcat cactttccga gttatatgtt tcaataactg tcctgacgaa gaagtgaacc
     1801 gaatctaccc ggcaagctac tcccttttca caggtatatt ctaacagact gtctcttaaa
     1861 aatcaattat tttttatcat aagattctcc ataatccata atatgttact gttcacacac
     1921 cactatcccg cgaggtgttt tggcatgaat atgccaaaat cccgagacat acaagccaaa
     1981 attctattgc cgcaggcaat ctggaatgaa ttttggctac gttactgaga acggagtgaa
     2041 cagtaatgta gcctcccctc tgtgtcagaa tctgcgtcag aaatctgcag tatttaaaat
     2101 ttcctgaggg atatatctgt ccttatccag aacagcttcc atctttttga ccattatttc
     2161 atcaaaacag gagaaaaatt gtgtactgat atttctgttc tgatgctcct ttgtctccgg
     2221 aagttcaatt atctgttctg cttttccaag catagcctga tatgccggca caaaaggaat
     2281 aattttccat ctgctgcgaa atttttctgc cagattttcg tatataccga tgccctcata
     2341 atccatatcc ccgaaataat aaattgtatt cttcggatgt ttcatatagg gttccgcaca
     2401 cagatcaaaa tcctgaaaag aacggataat accttttcct gcaccataaa tcagagttcc
     2461 gatttctgca ccaaaaattt cagtatgtcc attcagaaga t
//
LOCUS       DBMDXG010000332         2473 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9109_length_2473_cov_1.362142, whole genome shotgun sequence.
ACCESSION   DBMDXG010000332 DBMDXG010000000
VERSION     DBMDXG010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9109_length_2473_cov_1.362142"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            77..1225
                     /locus_tag="ACZPD3_08665"
     CDS             77..1225
                     /locus_tag="ACZPD3_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR1416093.1"
                     /translation="MLQKLYVFFRKETVLSIAVILALLSMFWVRPDKAYFTYIDFRTL
                     GLLFCLMAIVAGLKAVGVFDILAKKLLMGTSGTVGVIRLLVLLCFFLSMVITNDVALI
                     TFVPLALIIVHKLPKELGNYWLLKIVAMQTIAANLGSMLTPIGNPQNLYLYARAGMSA
                     AELITLMLPYSATALILLLIWIQVAAAKAPHVCGSEKNKTLLGFSDRKELNMEYLAAY
                     LILFTICLLTVARIIPYQIPLVLVLIYMLLRNRENISRVDYSLLATFIALFIFIGNLG
                     RIPQFSSFLERIMAGRETLTAVLASQIMSNVPAALLLSGFTDNYRALIVGTNIGGLGT
                     LIASMASLISFKYIAKENRNLRGKYLGIFTASNIIFMIFMLILYFFLR"
     gene            complement(1415..2182)
                     /locus_tag="ACZPD3_08670"
     CDS             complement(1415..2182)
                     /locus_tag="ACZPD3_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005330942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR1416094.1"
                     /translation="MGKIVQTAGRNTLGEFAPEFAHFNDDVLFGENWNNQDIDVKTRS
                     IITVVALMASGITDSSLRYHLQNAKNHGVTQKEIAAVITHAAFYAGWPKAWAVFNLAK
                     EVWETGEGDLPYEEEAMRAHAKEMVFPIGAPNDGFAQYFSGRSFLAPISTSQVGIFNV
                     TFEPGCRNNWHIHHAKSGGGQILVCVAGRGYYQEEGKDAVEMKPGDCINIPAEVKHWH
                     GAAPDEWFSHLAIEVPGENSSNEWLEPVSDEEYRKLK"
     gene            complement(2211..>2473)
                     /locus_tag="ACZPD3_08675"
     CDS             complement(2211..>2473)
                     /locus_tag="ACZPD3_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005426704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR1416095.1"
                     /translation="MDMSSYDEILLGFPIWWYVAPTIINTFLEAYDFSGKKIVLFATS
                     GGSGFGNTVKELQSSASDAVITEGRLLNCGTKQEITEWVNSL"
BASE COUNT          656 a          560 c          454 g          803 t
ORIGIN      
        1 aaaatgatgt tctttttttc tggatgtttc tgtgatatac ttttacgcgg tcgtatcaga
       61 ccggaaagga aatattatgt tacaaaaatt atatgttttt ttcaggaaag aaaccgtttt
      121 gtcaattgca gtgatcctgg cgcttctttc tatgttctgg gtcagaccgg ataaggctta
      181 ttttacttat atcgatttca ggactctcgg gctcctcttc tgtttgatgg caattgtagc
      241 tggtttgaag gctgttggtg tttttgatat acttgccaaa aaattgctga tgggaacatc
      301 gggaactgtg ggtgtgatca ggctgttggt actcttatgt ttcttcctgt caatggtgat
      361 caccaatgat gtggcattga ttacttttgt tcctcttgca ctgatcattg tgcataaact
      421 gccaaaggaa ctgggcaatt actggcttct taaaatagta gccatgcaga caatcgctgc
      481 taatctcgga agcatgctca caccaatcgg aaatccgcag aacctttatc tatatgccag
      541 agcagggatg tccgcggcag aattaataac cctgatgctt ccctattccg ccacagcact
      601 tatccttctt ctgatatgga tacaggtagc cgctgccaaa gcacctcacg tatgcggttc
      661 cgaaaaaaat aaaactcttt tgggattctc tgacagaaaa gaacttaaca tggaatacct
      721 ggccgcttac ctgatcttat ttacaatctg cctgctcact gttgcacgta tcattccata
      781 ccagattcct cttgtattgg ttcttatata tatgctgcta agaaacaggg aaaatataag
      841 cagggtggat tactccctgc ttgccacctt tatcgcatta tttattttta tcggcaacct
      901 tggaaggatt ccgcaattca gcagttttct ggaaagaatc atggcaggaa gggaaacact
      961 tactgctgtt cttgccagtc agattatgag caatgttccc gctgcgcttc ttttatccgg
     1021 ctttacagat aattaccggg ctttaattgt cggaaccaat attggaggtc tcggcactct
     1081 gattgcatcc atggcaagcc tgataagttt caaatatatt gcaaaagaaa acaggaatct
     1141 gagagggaag tatttgggaa tattcacagc ctccaatatt atattcatga tattcatgtt
     1201 aatattgtat ttttttctta gataaaggtt ttcccggaat tacaaaagct atagcaattc
     1261 tttcttcgca aaaaattctc cgcggcactc tcccatcctg gaagaaaagc cgcggagaaa
     1321 taaatatatt ttcggtgcca atacaaagat cattaatctt tacaccactt tacaactttc
     1381 aaatcgtctt ataaatatag ttttttcaac cttcctattt tagttttcta tattcttcat
     1441 cacttaccgg ttcaagccat tcattggaac tattttcgcc cggaacttct attgccagat
     1501 gtgaaaacca ttcatccggt gcggcgccat gccagtgttt cacttctgcc ggaatattga
     1561 tgcagtcgcc tggcttcatt tctacagcat ctttcccttc ttcctgataa tatcctcttc
     1621 cggcaacaca taccaggatc tgtcctcccc cactttttgc atgatggata tgccagttat
     1681 ttctgcatcc tggttcaaat gtcacattga aaattccaac ctgagaagta gaaatcggtg
     1741 caagaaaact cctgcccgaa aaatactgtg caaagccatc gtttggtgca ccaattggaa
     1801 ataccatctc ttttgcatgc gcccgcatcg cttcctcttc gtatggcaaa tctccctcgc
     1861 ctgtttccca cacttccttt gcaagattga aaacagccca tgcttttggc caacctgcat
     1921 agaatgcggc atgtgtgatc actgcagcaa tctctttttg tgtcacacca tgattttttg
     1981 cattttgaag atgatatctt aaagaagaat ccgtaattcc ggaagccatc agagcaacca
     2041 ctgtaataat gcttcttgtt tttacgtcga tgtcctggtt attccagttt tcgccgaaaa
     2101 gtacatcatc gttaaaatgt gcaaattccg gtgcgaattc accaagtgta tttcttcctg
     2161 ctgtctgtac tatttttccc atattctgta tacctccata acgctgttat ttataaagag
     2221 tttacccatt cagtgatttc ctgtttcgtt ccacaattta acagtctgcc ttctgtaata
     2281 actgcgtctg atgcagatga ctgcaattct ttcacagtgt tcccaaatcc acttcctccg
     2341 gatgttgcaa aaagcacaat ctttttacca ctgaaatcgt aagcttctaa aaatgtattg
     2401 atgattgtcg gagccacata ccaccagatt ggaaatccca gaaggatctc gtcataagaa
     2461 ctcatatcca tct
//
LOCUS       DBMDXG010000333         2468 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9124_length_2468_cov_1.587664, whole genome shotgun sequence.
ACCESSION   DBMDXG010000333 DBMDXG010000000
VERSION     DBMDXG010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9124_length_2468_cov_1.587664"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1838
                     /locus_tag="ACZPD3_08680"
     CDS             <1..1838
                     /locus_tag="ACZPD3_08680"
                     /inference="COORDINATES: protein motif:HMM:NF039514.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HIR1416096.1"
                     /translation="FTYNNTSLADDKKAFIYASIVGNRGRQPQTYADYRDHMKIFAMR
                     KAKEGRMNENYAVLYQEFLSNPKTEGQAKLLAQKLFTHRLYCDDKKIRYVIVRHEQLR
                     EEEIYPCIQGVAYPRIYTDDAVILFQDGKQRRYVSTVDYNIKKLFDERELTDKVLGFH
                     IEEPGLVLHCSEHTELKPENLQAFQRIPESEEFSDEYQKCIREKLLSYYSEHTRAEEA
                     DNYLRQMDYKKYAAVDRTALLEVLISRGMYQQAMSIVSQFGYEGIRIESQLKLTSRML
                     TRCEMEEDDELLALASDVYRRGKYDEVILKYLMEYRFGPVDELISVWKSAQGFEMDTY
                     ELEEKLLGLLMFTSDYRKEGEKILEDYVHHSGKERITGAYLTQTAYGAFVKEYPMSVF
                     VRSLLERAYDEKWPVDFVCSLALLEAYSKEKKLEKKQLCNAEEILQKCVKQGMYFAFF
                     RKLPASVLSPYQLDDKTFVEYHADPSAKVTLYYALDAGLGNQVKYQTEPLRDLYEGIF
                     AKSFTLFYGETLRYYFESVTGERVNRTQERVLTMNKIEGTPVSKYHMINQMLSARRLD
                     KKKEVFSQVKKYLRQEQYVKKMFVIEKEEQEQIVLKPGGTNERNS"
     gene            1822..>2468
                     /locus_tag="ACZPD3_08685"
     CDS             1822..>2468
                     /locus_tag="ACZPD3_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HIR1416097.1"
                     /translation="MNEIRDLIVGIDFGKEYSQICYYDRKGDEARSLSMKAGGNQYEA
                     PCILCWRPEQKDYCVGLEADYFVREKGGVMIDDVYGICEKSDSVLVEGQELAPWEILA
                     QFLQGMLKFLGVAELVKNTKCVAVTLPGLTEIQVENFQKAFEIIGFPHEKYMLLDYGE
                     SFYYYVLSQKRETWNRSVGWYAFTPENVSFRKMTMNGATKPVTVKLEEAVETELPA"
BASE COUNT          781 a          407 c          645 g          635 t
ORIGIN      
        1 attttaccta taataatact tctcttgcag atgataaaaa ggcatttatt tatgcaagta
       61 ttgtgggaaa cagaggccgt cagccacaga catatgcgga ttacagggat catatgaaga
      121 tttttgccat gcgcaaggcg aaagaaggac ggatgaatga gaattatgcg gtcctttatc
      181 aggaattttt aagcaatccc aaaacagaag gacaggcaaa actgctcgcg cagaagctgt
      241 ttacacacag actgtactgt gatgataaga agatacgcta tgtgatcgtg cgccatgaac
      301 agctcaggga agaagaaatt tacccatgta tccagggcgt agcttatcca agaatttata
      361 cagatgatgc ggtgatcctg tttcaggatg ggaagcagag aagatatgta tcaacagtag
      421 attataatat caagaaatta tttgatgagc gtgaactgac agataaggtt cttggttttc
      481 atattgaaga gccgggactg gtacttcact gcagtgagca tacagaactg aaaccggaga
      541 atcttcaggc attccagagg atcccggagt cagaggaatt ttcagatgaa tatcagaaat
      601 gtatcagaga gaagctgctt tcttattatt ccgaacacac cagagcagaa gaagcagata
      661 attatctgag acagatggat tataagaaat atgcggctgt agacagaaca gctcttctgg
      721 aagtactgat ctccagagga atgtatcagc aggcaatgtc cattgtttcc caattcggat
      781 atgaaggaat ccgcatagag agccagctga agctgaccag cagaatgctg acaagatgtg
      841 agatggagga ggatgacgaa ttgcttgccc ttgcatcgga tgtatacaga cgcggaaaat
      901 atgatgaagt gatcctgaag tatcttatgg aatacagatt tggtcctgta gatgaactga
      961 tatctgtatg gaaaagtgcg cagggctttg agatggatac ttatgaactg gaagagaagc
     1021 ttcttggcct tctgatgttt acttctgatt acaggaaaga gggagagaag atcctggagg
     1081 attatgttca tcacagcgga aaggaacgta ttacaggagc atatcttacg cagactgcat
     1141 atggtgcatt tgtaaaagaa tatccgatga gtgtttttgt aagaagtctg ctggagcggg
     1201 cttatgatga gaagtggccg gttgattttg tatgcagtct tgctcttctg gaagcgtatt
     1261 ccaaagaaaa aaaactggag aaaaaacagc tttgcaatgc agaggagatt cttcagaaat
     1321 gcgtgaaaca gggaatgtac tttgcgttct ttagaaaact tccggcatct gtcttaagtc
     1381 cctatcagct ggatgataaa acctttgtgg aataccatgc agatccgtct gcaaaggtga
     1441 cactctatta tgcactggat gcaggcctcg gaaatcaggt gaaatatcag acagagcctt
     1501 tgcgtgattt atatgaaggt atttttgcaa aatcatttac attgttctat ggggagacat
     1561 tgcggtatta ttttgaatca gtaacaggag agcgggtaaa tcggacacag gagagggtac
     1621 ttacaatgaa taagattgag ggtacacctg taagcaaata tcatatgatc aatcagatgc
     1681 tctctgcacg cagactggat aaaaagaaag aagtgttttc acaggtgaag aagtatcttc
     1741 gccaggaaca gtatgtgaag aagatgtttg tgatcgagaa agaagagcag gaacagattg
     1801 tcctgaaacc gggaggaaca aatgaacgaa attcgtgatt tgatcgtggg aattgatttt
     1861 ggcaaagagt attctcagat atgttattat gacagaaaag gagacgaggc acgttctctt
     1921 tctatgaaag cgggtggaaa tcagtatgag gctccctgta ttctgtgctg gagaccggag
     1981 cagaaagact attgcgtagg actggaagca gattactttg tccgggaaaa gggcggagtc
     2041 atgatcgatg atgtctatgg aatctgtgaa aaaagtgatt ctgtgctggt ggaaggacag
     2101 gaacttgcac catgggagat cctggctcag tttctgcagg gaatgcttaa atttctggga
     2161 gtggcagaac tggtcaaaaa tactaaatgt gtggcggtga cactgcccgg gctgacggaa
     2221 attcaggttg agaatttcca gaaagcattt gagataattg gatttccaca tgagaaatat
     2281 atgctgctgg attatgggga aagcttctat tattatgtgc tctcacagaa aagagaaacc
     2341 tggaaccgca gtgtgggatg gtatgccttt acaccggaga acgtgtcttt tcgcaagatg
     2401 acaatgaacg gtgcaacaaa gcctgtcact gtaaaactgg aagaagctgt ggagacagaa
     2461 cttcctgc
//
LOCUS       DBMDXG010000334         2454 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9177_length_2454_cov_1.239269, whole genome shotgun sequence.
ACCESSION   DBMDXG010000334 DBMDXG010000000
VERSION     DBMDXG010000334.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9177_length_2454_cov_1.239269"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            261..1643
                     /locus_tag="ACZPD3_08690"
     CDS             261..1643
                     /locus_tag="ACZPD3_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR1416098.1"
                     /translation="MKKNLHIISRVLPDSIGEELELEPGDALLSINGQPVEDVFDYRY
                     LMNDEFVTLLIRKKNGEEWELEVEKEYEDDLGVEFENSLMDEYRSCSNHCIFCFIDQM
                     PPGMRETLYFKDDDSRLSFLQGNYVTLTNMSDYDLDRIIKFHLSPINVSFQTMNPKLR
                     CKMLHNRFAGDALAKVDRLYKGDVTMNGQIVLCKGINDRDELEYSLEKLSEYAPVLQS
                     VSIVPVGLSRYRKGLYPLESFDKEDARYLISQVERWQKIMVKKHGIHFVHASDEWYIL
                     AGYELPEEGRYDGYLQLENGVGMMRLLETEVKERLEQLEGDDREVNATVATGRLAAPY
                     IGKMIKLVQKKFPNVQAEVYAVKNNFFGEKITVSGLITATDLMDQLAQRNLGEKVLIP
                     CNMLRSGEDVFLDDLTVEDVRQALKTEVVVVDEPGADLVNCLIEVPDHKKIRRRQIYE
                     QTGSSDSGQT"
     gene            1606..>2454
                     /gene="der"
                     /locus_tag="ACZPD3_08695"
     CDS             1606..>2454
                     /gene="der"
                     /locus_tag="ACZPD3_08695"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR1416099.1"
                     /translation="MSKPVVAIVGRPNVGKSTLFNALAGEKISIVKDTPGVTRDRIYA
                     DVTWLDKTFTMIDTGGIEPDSSDIILSQMREQAQIAIDTADVIIFITDVHQGLVDSDA
                     KVADMLRRSGKPVVLVVNKVDSIQKFMMDVYEFYNLGIGEPIPISAANRMGLGDMLDE
                     VAKHFPEDADTEEDDEIPRIAIVGKPNVGKSSLVNKLLGEDRVIVSDIAGTTRDAVDT
                     RVKWQGKDYIFIDTAGLRRKGKVKEEIERYSVIRTVTAVERADVVIVMIDASEGVTEQ
                     DAKIAGI"
BASE COUNT          749 a          419 c          638 g          648 t
ORIGIN      
        1 ataacatttg ccctaaaata cgtcaaggaa tttattgatt ttttctttta caattgttat
       61 gatataaaag atagtggatt ctgggcgcag gtatattttg tccggaatta ctgtcatatg
      121 atatcaggag taaatgtcag aaagttactg tgcctgcaca gtaaataaat cagcggctcc
      181 tgaatgatcg agaaacaaaa tgtgccggaa aagtttatcc cacacacaat ttccggttac
      241 attccaatgg agtaaaattc atgaaaaaaa atttacacat aatatccaga gttcttccgg
      301 acagcatcgg agaagaactg gaactggaac cgggagatgc gctgctttcc attaacggcc
      361 agccggtaga ggatgtgttt gattatcgtt atctgatgaa tgacgaattt gttacgctcc
      421 ttatccgaaa gaagaatggc gaggaatggg aactggaagt agaaaaggaa tatgaagatg
      481 atcttggcgt ggagtttgag aacagtctta tggatgagta ccgctcctgt tccaatcact
      541 gtattttctg ttttatagac cagatgccgc ccggtatgag agaaacttta tattttaaag
      601 atgatgattc cagactgtca ttcctgcagg gaaactatgt tacccttacc aatatgagtg
      661 actatgatct ggatcgtatt atcaagtttc atctttcccc gatcaatgta tcgtttcaga
      721 ctatgaatcc taagcttcgc tgtaaaatgc tccataatcg atttgccgga gatgcccttg
      781 caaaagttga caggctttat aaaggtgatg tgaccatgaa cgggcagatt gtgctctgca
      841 aggggattaa tgacagggac gaactggaat acagtctgga aaaactgtcg gaatatgcgc
      901 ctgtactgca gagtgtttca atcgtacctg taggcctttc aaggtatcga aaaggtctgt
      961 atccactgga atcttttgat aaagaggatg caaggtatct gatcagtcag gtagagcggt
     1021 ggcagaagat catggtgaag aagcatggta ttcattttgt acatgcttcg gatgaatggt
     1081 atattcttgc aggatatgaa cttcctgagg agggcagata tgacggatac cttcagctgg
     1141 agaatggtgt tggtatgatg cgtctgcttg aaacagaggt aaaagagaga cttgagcagc
     1201 ttgaaggcga tgacagagag gtaaatgcca ctgttgcaac aggacggctg gcagcgcctt
     1261 atataggtaa aatgataaaa ctggtacaga agaaatttcc gaatgtccag gctgaagtat
     1321 atgcagtaaa gaataatttc tttggagaga agatcacagt ttccggtctg attacagcta
     1381 ctgatcttat ggatcagctt gcgcagagaa atctgggaga aaaagtactg atcccatgca
     1441 atatgctcag aagcggcgag gatgtttttc tggatgacct gacggtggaa gatgtcagac
     1501 aggctcttaa gacggaagtt gtagtggtag atgaaccggg tgctgacctg gtaaactgtc
     1561 tgatcgaagt accagaccat aaaaagataa gaaggagaca gatatatgag caaaccggta
     1621 gtagcgatag tgggcagacc taatgtaggt aaatccacat tgtttaatgc gctggcaggt
     1681 gagaagattt ctattgtaaa agatacaccc ggcgttacaa gagacagaat ttatgcagat
     1741 gtgacatggc tggacaagac atttaccatg atcgatacag gcggtattga gccggacagc
     1801 agtgacatca tcctttctca gatgagagag caggcacaga ttgccattga tacggcagat
     1861 gttatcatct ttatcacaga cgttcatcag ggactggtgg attccgatgc gaaggtggca
     1921 gatatgctcc gacgcagcgg caaaccggtt gtccttgttg tgaataaggt agacagtatc
     1981 cagaaattta tgatggatgt ttatgagttc tataaccttg gtatcggtga accgattccg
     2041 atttctgcgg caaaccgtat ggggctgggt gatatgctgg atgaagtggc aaaacatttc
     2101 cctgaggatg cagatacaga agaagatgat gaaatcccga gaattgccat tgtgggaaaa
     2161 ccaaacgtag gaaaatcgtc attggtaaac aaacttctgg gagaagaccg tgtgattgtt
     2221 tctgatattg ccggaactac aagagatgcg gtagatacca gagtaaaatg gcagggaaag
     2281 gattatatct ttattgatac tgccggactt cgccgtaagg gaaaagtcaa agaggagatt
     2341 gagagataca gtgtgatccg tacagtgact gcagtagaac gtgcggatgt tgtcattgta
     2401 atgatcgatg cgtcagaggg tgttacagaa caggatgcca agattgcagg tatt
//
LOCUS       DBMDXG010000335         2439 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9234_length_2439_cov_1.791702, whole genome shotgun sequence.
ACCESSION   DBMDXG010000335 DBMDXG010000000
VERSION     DBMDXG010000335.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9234_length_2439_cov_1.791702"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2216)
                     /locus_tag="ACZPD3_08700"
     CDS             complement(<1..2216)
                     /locus_tag="ACZPD3_08700"
                     /inference="COORDINATES: protein motif:HMM:NF017183.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HIR1416100.1"
                     /translation="MKSNYKIFTAVFLSAIMTAAAPLTVCGAEVFSAGESFSDGTGSI
                     PAILTDMVLPDGEIGTEYQVQLEADTDVNWSLEEGSELPAGFTLSSGGVISGMPQEEG
                     SFSFTVQADNGIGVSSQELSLYVAQREQEPVIKQYELTSGTALIDLGIHAEGEQSRKR
                     LFLYNAGTDSLHLSDFPQSRYFDFSYAPIFDTNTDEEMQPGSCAAVDIDDKKNLPAGN
                     YEETVTFETKEGASCKVTLKVIVGASSEKDYELSIETGTEEEFTESDFYDPEFRVEKY
                     VVVRNSGKKETRIRIDTSGLKNFEVDDQMAKWTEDYEEQDSKKLLKPGETLYFYILPK
                     QEYGIYDETFAFLADDGSRYPLHVTMNREKNPTEKKQLEITEKSAGGFPTMQWGYKTL
                     PEAKTYILKNVTDTDMKLSFNRSKECSVLLSGNAYLAPGESTELRLWPKLGLSVGKYS
                     FQVTVTAKTVAGEHLTTQNLYNSFIVGDRTYQGLADAVAPVTGITNGAEKTADALHLP
                     KSLEVYGAEVNGEKTTFSASVKWDVENCAYDPKSKAAQSFLVNGSLELNEDENNEGLD
                     TAVQIMVQVDAYQTLNRPVIDTRWTRVITNYAMLYLRDLSNEADGYQFVTAKSQKDLK
                     KGNYTAQTKIANSELNQYPELKYIPEGTHSLYCRAYKENSSHVMEYGEWSDGVPVTVK
                     VKTPDAPVVQKVQVKKNDVRIVLNSKGEEPDGYDVVAYRKVRRTPDWCRYHSGHSG"
BASE COUNT          638 a          639 c          401 g          761 t
ORIGIN      
        1 ctaccggaat gccctgaatg gtatctacac caatctggcg tccgacgaac tttacggtaa
       61 gcaacaacat cgtaaccgtc cggttcctca cccttgctgt taagtactat tctgacatca
      121 tttttcttca cctgcacctt ctgaaccaca ggtgcgtccg gagtctttac ctttaccgta
      181 accggaaccc cgtctgacca ttccccatat tccataacat ggctgctgtt ctctttgtat
      241 gccctgcaat aaagactatg tgttccctcc ggaatatact tcagctcagg atattgattc
      301 agttcagaat ttgcaatttt tgtctgcgca gtataattcc cttttttcag atccttctga
      361 gatttcgctg tcacaaactg atagccgtcc gcttcattgg aaagatctct cagataaagc
      421 attgcataat ttgtgatcac tctggtccac cgcgtatcaa taactggtct gtttaatgtc
      481 tgataagcat ccacctgtac cattatctgt acggcagtgt ccagcccttc attattttca
      541 tcctcattca gttccagaga tccgttgacc agaaaacttt gagctgcctt gcttttcgga
      601 tcataagcac aattctccac atcccacttc acagacgcag aaaatgttgt tttctctcca
      661 ttcacctcgg cgccatagac ttccagcgac ttcggcagat gcaaagcatc agcagttttt
      721 tctgcaccat tggtaattcc ggtcacaggc gcaacagcat ctgccagtcc ctgataagtc
      781 cggtctccta caataaaaga attatacaga ttctgcgttg tcaggtgttc tcctgccact
      841 gttttcgcag tcacagtcac ctgaaaacta tattttccca ccgaaagccc cagtttgggc
      901 cagagtctca gttctgtgct ctccccaggt gccagataag catttccaga taataacaca
      961 ctacattctt tactacggtt aaaagacagc ttcatatctg tatcagtaac atttttcaga
     1021 atatatgtct ttgcttccgg aagtgtctta tagccccact gcattgtcgg aaaacccccg
     1081 gcagactttt cagtaatttc cagctgcttc ttttctgtcg gattcttctc tctgttcatt
     1141 gtaacatgaa gaggatatct gcttccgtca tctgcgagaa atgcaaacgt ttcatcataa
     1201 attccgtatt cctgcttcgg caatatataa aaataaagcg tttctcccgg tttcagcagc
     1261 tttttggaat cctgttcctc ataatcttct gtccactttg ccatctggtc atccacttca
     1321 aaattcttaa gaccagaagt atctatccgt attctggttt ccttttttcc tgaatttctg
     1381 accactacat acttctccac acgaaattcc gggtcataga aatcgctttc tgtaaattct
     1441 tcttctgtac ctgtttctat actcagctca taatcttttt cagaggaagc tcccacaatt
     1501 actttcagag taaccttaca ggaagcaccc tcttttgtct caaatgtcac tgtctcttca
     1561 taatttccgg caggcagatt cttcttatca tcaatgtcca cagccgcaca acttccgggc
     1621 tgcatttctt catccgtatt tgtatcgaaa atcggtgcgt aggaaaaatc aaaatatctg
     1681 ctctgcggaa aatcagagag atgaagcgaa tctgttcctg cattataaag aaataaccgc
     1741 ttacgtgact gttctccctc tgcatgaatt cccagatcga tcaaagcagt acctgatgtc
     1801 agctcatact gctttataac aggttcctgc tcacgctgtg caacataaag gctcagttcc
     1861 tgtgaagaaa ctccaatacc attgtccgcc tgtacagtaa agctgaaact cccttcctcc
     1921 tgtggcattc cactgatcac tcccccggaa gacaacgtaa atcccgccgg aagctcactt
     1981 ccctcttcca gactccagtt cacatctgta tcagcttcaa gctgtacctg atattccgtt
     2041 ccaatctctc catccggcag aaccatatct gtcaatatgg caggaatact ccccgttcca
     2101 tcactgaaag attctcccgc cgaaaaaact tccgccccac atactgttaa cggagctgct
     2161 gctgtcataa ttgccgataa aaaaactgct gtaaaaattt tgtaattact tttcattctt
     2221 ctcctccaga taataaaata taaaagtatc aatttaccta attaatatta atataccact
     2281 ggacgggaat gtttctgtaa ttacggaatg acatgttgta taatggtcat gaatatcaga
     2341 aaagccagta atgtttcggg tatatgtcaa aaacattact ggtctttttt ataagattat
     2401 tccacacttt ttattcgata cagggaatct cataattcc
//
LOCUS       DBMDXG010000336         2436 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9249_length_2436_cov_1.371734, whole genome shotgun sequence.
ACCESSION   DBMDXG010000336 DBMDXG010000000
VERSION     DBMDXG010000336.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9249_length_2436_cov_1.371734"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="ACZPD3_08705"
     CDS             complement(<1..1305)
                     /locus_tag="ACZPD3_08705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR1416101.1"
                     /translation="MQDRKAPADKEKNGIQVPFKSAIKNSSRRPKKPTSKLKIIPLGG
                     LEQIGMNITAFEYEDSIVVVDCGLAFPEDDMLGIDLVIPDVTYLKENISKVKGFVITH
                     GHEDHIGALPYVLKDVNVPVYSTKLTIALIANKLKEHNMTNTTKLKEVRHGQVINLGD
                     FSIEFIKTNHSIQDASALAIYSPAGIVVHTGDFKVDYTPVFGDAIDLQRFAEIGRKGV
                     LALMCDSTNAERTGFTMSERSVGHVFDNLFNEYKTNRIIIATFASNVDRVQQIINTAY
                     RFGRKVAVEGRSMVNVITTAAELGYLRIPDQTLIEIDQVKNYPDEQVVLITTGSQGES
                     MAALSRMAANIHKKIVIKPNDTIIFSSNPIPGNEKAVSKVINELSMKGAKVIFQDVHV
                     SGHACQEEIKLIYSLVKPKYAIPVHGEYRHLTAQKNVVEELGI"
     gene            complement(1469..1726)
                     /locus_tag="ACZPD3_08710"
     CDS             complement(1469..1726)
                     /locus_tag="ACZPD3_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR1416102.1"
                     /translation="MEKIKFQLEDGTEAEFYVEEQTRIGGVAYLLVSDSLDDEATAYI
                     FKDVSEDDSQEACYEMVEDEDEMQAVFKVFEQMLDDVDLEM"
     gene            complement(1739..2161)
                     /gene="ruvX"
                     /locus_tag="ACZPD3_08715"
     CDS             complement(1739..2161)
                     /gene="ruvX"
                     /locus_tag="ACZPD3_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR1416103.1"
                     /translation="MRILGLDYGSKTVGVAISDPLGITAQGVETIWRKQENHLRQTLA
                     RIEELVSEYHVEKIVLGYPKNMNNTIGERAQKSLEFKEMLETRTGLEVIMWDERLTTV
                     EANRTLMEASVRRENRKQYLDQLAAVFILQGYLDSLSC"
     gene            complement(2163..2435)
                     /locus_tag="ACZPD3_08720"
     CDS             complement(2163..2435)
                     /locus_tag="ACZPD3_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR1416104.1"
                     /translation="MAENNLGNTQYFRTKPDQELQVKEVLDLVYTAMDEKGYNPVNQI
                     VGYIMSGDPTYITSHKGARSMIMKVERDELVEELLNEYIKNKSWER"
BASE COUNT          611 a          594 c          453 g          778 t
ORIGIN      
        1 gattccaagt tcctcaacta catttttctg tgcggtcaga tgtctgtatt caccatgaac
       61 agggattgca tatttcggtt ttaccagaga atagatcagc ttgatttctt cctggcaggc
      121 atgtccggaa acatggacat cctggaaaat aaccttcgca cccttcatgg aaagctcatt
      181 gataaccttg gataccgctt tctcatttcc cggaatcgga ttggaactga aaataatagt
      241 atcattcggt ttgatcacta ttttcttatg gatgtttgca gccatacggg aaagagctgc
      301 catggattca ccctgacttc ctgttgtgat cagcactacc tgttcatccg ggtaattctt
      361 aacctgatcg atctcaatca gagtctgatc cggaatgcga aggtatccaa gctctgctgc
      421 agttgtgata acatttacca tactgcggcc ctctactgca accttgcgtc caaaacgata
      481 agctgtgtta atgatctgct gtacgcggtc tacattggat gcaaaagtgg caataatgat
      541 tctgtttgtc ttatattcat tgaaaagatt atcaaacaca tgtcccacag aacgttcaga
      601 catggtaaat ccggttctct ctgcattagt actgtcacac atcagtgcca gtactccctt
      661 tcttccaatc tctgcaaaac gctgcaaatc aatggcatct ccgaatacag gagtataatc
      721 tactttaaag tcgccggtat gcactacgat gcctgccgga gaatagatcg caagtgcaga
      781 tgcatcctgg atactatggt ttgtcttgat aaattcaatt gaaaaatcac caagattaat
      841 gacctgtcca tgccttactt cctttaactt ggtagtattg gtcatattat gctctttcag
      901 cttatttgca ataagggcaa tggtcagctt cgtggaataa acagggacat ttacatcttt
      961 gagtacatac ggcagcgcac cgatatggtc ctcatgtcca tgagtgatca caaagccttt
     1021 tactttggaa atattctctt ttaaataggt gacatccgga atgacaaggt caattccgag
     1081 catatcatct tccgggaaag caagaccgca gtctaccaca acaatgctgt cttcatattc
     1141 aaatgctgtg atattcattc caatctgttc cagaccaccc agaggaatga tctttaattt
     1201 agatgttggt ttcttcggtc tgcgggaaga attctttatc gcagacttaa atggtacctg
     1261 tattccgttc ttctctttat ctgccggagc cttgcggtcc tgcattggtt ttcttccata
     1321 agagtttctg tgctgtggtt tatatttata attcttcggt ctgttgtatg gcctcttctg
     1381 accatgtccg aactgtccgt catgattttc acgcgcttca ttattaaact ggttgttatt
     1441 ttcgttactc aagtactaca cctccatgct acatttcaag gtctacgtca tctaacattt
     1501 gttcaaatac tttaaaaaca gcctgcatct catcctcgtc ctcgaccatt tcatagcagg
     1561 cttcctgact gtcatcctct gacacgtctt taaaaatata ggccgtcgcc tcatcatcca
     1621 gagaatcaga taccagaaga tatgcaactc ctccgatcct ggtctgctcc tctacataaa
     1681 actccgcttc tgttccgtcc tcaagctgaa atttaatttt ctccatctta ttaatctcct
     1741 aacaggacag ggaatccaga tatccctgca aaataaaaac agcagccagc tgatccagat
     1801 actgcttgcg gttctctctg cgcacactgg cttccattaa agtacgattg gcttctactg
     1861 ttgtcagacg ctcgtcccac atgatcactt caagacctgt acgtgtctcc agcatttctt
     1921 taaattccag tgatttttgg gcacgttctc caatcgtatt gttcatgttc ttcggatatc
     1981 caagcacgat cttctccacg tgatattccg aaacaagttc ttcaatacgg gcaagtgtct
     2041 ggcgcaggtg gttctcctgt ttacgccaga tcgtctctac accctgagcg gtgatcccca
     2101 gcggatcact gatggctaca ccgactgttt ttgatccata atctaatccc agaattctca
     2161 tatcagcgtt cccaggattt gttcttaata tactcgttta aaagttcctc taccagttcg
     2221 tcgcgctcta ccttcatgat catgcttctt gcgcccttgt gactggtaat ataagtagga
     2281 tctccggaca taatatatcc aacgatctga ttgaccggat tgtatccttt ctcatccatt
     2341 gctgtgtaaa cgagatccag aacttctttt acctgaagct cctggtctgg ttttgttctg
     2401 aaatattggg tattacccag attgttctct gccatt
//
LOCUS       DBMDXG010000337         2429 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9271_length_2429_cov_2.928694, whole genome shotgun sequence.
ACCESSION   DBMDXG010000337 DBMDXG010000000
VERSION     DBMDXG010000337.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9271_length_2429_cov_2.928694"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="ACZPD3_08725"
     CDS             <1..174
                     /locus_tag="ACZPD3_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HIR1416105.1"
                     /translation="EFGLKVSSLYISQVKRKCGIEVGENYNLPKSENARVPQCPKEKE
                     DAIKAALKYYAMI"
     gene            206..577
                     /locus_tag="ACZPD3_08730"
     CDS             206..577
                     /locus_tag="ACZPD3_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HIR1416106.1"
                     /translation="MKSTFEKMGGTYTLGADGIYYPNLVSTDEEPHYGKYGMLRKTYL
                     KEHRPAMYSLYMLEDRLTEHLNAVDDETQEKMDILVSQMMEKQGITEELKARDQMEWV
                     RAVNNVRNAAEEIVLKELIYR"
     gene            complement(655..2364)
                     /gene="mobQ"
                     /locus_tag="ACZPD3_08735"
     CDS             complement(655..2364)
                     /gene="mobQ"
                     /locus_tag="ACZPD3_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HIR1416107.1"
                     /translation="MAIYHMQAKVVSRGSGRSAVAASAYMSCSRMYNDYDGIQHDYTR
                     KQGLIYQEVMLPPMAPLEWNDREQLWNAVEENEKTKDSRLAREFVVALPFELDKDSNI
                     SLLQDFIKKNFVDMGMCADFAIHDTDGHNPHAHILLTVRPLNENGTWQYKTEKEYLCI
                     KDGEEKGFTASEFKTAQKQGWEKQYRYKVGKKKEYLTSSVAQEKGYERIDKHPKSSRY
                     GRQNPISQQWNSDEQLCIWRANWADAVNKMLARNQINATIDHRSFADQGITEQPTIHE
                     GYIAQNMEKKGMIADRCEINRQIRADNKMLRELKAKVAKLAEAVEKSIPIIAETLEAI
                     RNHMIFIQYHLLHNEMQKEVIHDWMNHFNPILNKYNTVKKELKAKVTERKELNVQKDK
                     TSILNPIRHIKLNQQLTTITEEIEELKSRKEQLIFQAECSTNKDMTNLSKKYDQMNKN
                     LDILYSQDTSLKKQLEKDAAAFREEKFRPEPEQYTELLDTRIQIRPDFRDKLIEQLKG
                     TFGKYYDYHRRDIAANEVDYLNVEDPDVFSHRAWELEYQRKQEIRRNQPARTKKRSYD
                     MEL"
BASE COUNT          659 a          481 c          518 g          771 t
ORIGIN      
        1 gaatttggct tgaaggtttc aagtttatat atttctcagg taaaacgcaa atgtggaatt
       61 gaagtgggag aaaactataa tcttccaaaa tcagaaaatg caagagttcc acaatgcccg
      121 aaagagaaag aagatgctat caaggctgcc ctgaaatatt atgcgatgat ctaaggacat
      181 cgctgatttc aaggaggaat aacacatgaa aagcacattt gaaaaaatgg gtggaaccta
      241 cacacttggc gcagacggaa tttactatcc gaatcttgtc agtacagatg aagaaccgca
      301 ttatgggaaa tacggaatgt tgcggaaaac atatctgaaa gagcatcgtc cggcaatgta
      361 ttcactgtat atgttggaag acagattgac agaacatctg aatgcggtgg acgatgaaac
      421 acaggagaaa atggatattc tggtgagtca gatgatggag aagcagggca ttacggaaga
      481 attgaaagct cgtgaccaga tggaatgggt tagagcggta aataatgtcc ggaatgctgc
      541 agaagagatt gtgttgaaag aattgattta caggtaaaca aagggctgaa caggtaggac
      601 gaaaaatcct atttgttcag ctcttgtgtt tataatgggc aaagaagaac caaattataa
      661 ttccatatca tacgaccttt tcttagttcg agcaggctga tttcgccgta tttcctgttt
      721 cctctgatat tcaagttccc aggcacgatg ggaaaaaaca tcaggatctt ccacattgag
      781 ataatctacc tcattggctg caatatcacg gcggtgatag tcataatact taccaaaagt
      841 acctttgagc tgttcgatca gcttatcccg aaaatcagga cgtatctgga ttcgggtgtc
      901 cagcaattct gtatactgtt ctggttcagg acgaaatttt tcttcccgga aagcggcggc
      961 gtctttttca agctgctttt tgagcgaggt gtcctgagaa tacaggatat ccagattttt
     1021 attcatctgg tcatatttct tggatagatt cgtcatatct ttattggtag agcattctgc
     1081 ctggaagatt agctgctctt tccttgattt cagttcttcg atttcttcgg taatagtggt
     1141 aagctgctga tttaatttta tatgccggat gggattcaaa atactggttt tatccttttg
     1201 cacattcagt tctttccgtt ccgtaacttt agctttgagt tcctttttaa ctgtgttgta
     1261 tttattcagt attggattga aatgattcat ccagtcatgg atcacttctt tttgcatctc
     1321 attatgaagt aaatgatact gtataaaaat catatgattg cggatcgctt ctaacgtttc
     1381 agctattatt ggaatagact tttctacagc ttcagccagt tttgctacct ttgcttttaa
     1441 ttctcggagc attttgttat ccgcacgaat ctgacggttg atttcacagc ggtctgctat
     1501 catgcctttc ttttccatat tctgggcaat gtagccttca tggatggttg gctgttcggt
     1561 gattccctga tctgcaaagc tgcggtgatc aatggtagca tttatctgat tacgtgcaag
     1621 cattttattt acggcatctg cccagtttgc ccgccagata caaagttgtt catcactgtt
     1681 ccattgttgg gaaataggat tctgtctgcc atatcggctg ctcttggggt gtttatcaat
     1741 tcgttcatat cctttttcct gtgcaacaga agaagtcaga tattctttct ttttcccaac
     1801 tttatagcga tattgctttt cccatccctg tttctgagca gttttaaatt cagaagcagt
     1861 aaaccctttt tcttccccat ctttgataca gagatattct ttttctgttt tatactgcca
     1921 tgttccattt tcatttaatg gtcggacagt aagaagaatg tgtgcatggg gattgtgacc
     1981 atctgtatcg tgaatggcaa aatcagcaca catccccata tctacaaaat tttttttaat
     2041 aaaatcttga agaagagaaa tattgctgtc tttatccaac tcaaagggga gtgcgacaac
     2101 aaattctctt gcaagtcggc tgtctttggt cttttcgttt tcttccacag cattccagag
     2161 ttgttcccgg tcattccatt ccaatggagc cattggagga agcatcacct cctgatagat
     2221 cagcccctgc tttctggtat agtcatgctg gatgccgtca taatcattgt acatgcggct
     2281 acagctcata taagcagaag cagcaacagc agaccgaccg gaaccgcggc tgacgacttt
     2341 ggcttgcata tgataaattg ccatatctgg cacctccttt cgatgttgcc tgcaacagcg
     2401 gataaagccc tcggcagagc gcacgagcc
//
LOCUS       DBMDXG010000338         2428 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9278_length_2428_cov_2.067899, whole genome shotgun sequence.
ACCESSION   DBMDXG010000338 DBMDXG010000000
VERSION     DBMDXG010000338.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9278_length_2428_cov_2.067899"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     misc_feature    127..269
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            311..814
                     /gene="rplJ"
                     /locus_tag="ACZPD3_08740"
     CDS             311..814
                     /gene="rplJ"
                     /locus_tag="ACZPD3_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR1416108.1"
                     /translation="MAKVELKQPIVDEIINNVKDAESVVLVNYSGLTVEQDTALRKEL
                     REAGVVYKVYKNTMMHRAFEGTQCEELIQHLHGTNAIAISATDATAPARILDKFAKKA
                     PALELVAGIVEGNYNDQAGIQALAGIPSREELLGKLLGSIQSPITNFARVLNQIAEKN
                     GSEAAAE"
     gene            925..1296
                     /gene="rplL"
                     /locus_tag="ACZPD3_08745"
     CDS             925..1296
                     /gene="rplL"
                     /locus_tag="ACZPD3_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR1416109.1"
                     /translation="MAKLTTEEFIAAIKELSVLELNDLVKACEEEFGVSAAAGVVVAA
                     AGAAEAAEEKDEFDVELAEVGPNKVKVIKVVREVTGLGLKEAKEMVDGAPKVVKEGAS
                     KAEAEDIKTKLEAEGAKVNLK"
     gene            1384..>2428
                     /locus_tag="ACZPD3_08750"
     CDS             1384..>2428
                     /locus_tag="ACZPD3_08750"
                     /inference="COORDINATES: protein motif:HMM:NF024627.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR1416110.1"
                     /translation="MMKRKTAVIFGIIIAAQLCLIPYAGVKVQAAELEEGQMFEDSSE
                     DSETFGDVSIPDTQSAEKTEENEFGDDDGFSDGDVETFSAEDADQSCENMQELVLNVQ
                     DGEDITVKLNTLLAQARDRATDEKQCKVIVPPGNYTLTGTLHMYSNIYLYAEGATITK
                     TSPRKEILLRLGDTQKSAGGYEGYRNITIDGGTWDSNYECVEDKGGPGGFVGFRIGHA
                     TNVTVKNVTFLNNLKSHFLELAGVKNAEITGCTFRGYWKEFTGGGQECIQLDACMPRI
                     FPGYLPYDGSVCENIVIKDNTFEDVFAGIGSHSMMFDKPYKNITISNNRFNNLKKRAI
                     WCLNYQDTVVTGNT"
BASE COUNT          819 a          437 c          575 g          597 t
ORIGIN      
        1 attcccattt tcgacggact ggacaaacgc tacaagatgc gtaaggcaac cattgacata
       61 gagaacatca aactcgctca acaaaaaacc gaaaaaacct cttgacaacc caaatctcat
      121 atgctatctt ataccagaac gctgccgaag acagtaggtg ccgcaaggca taagttgtaa
      181 acgcctaccg aggactaagt attctttata tttttatgta agattacgca agccctactg
      241 ccagacagta aggcttttta tttactcatt tgcagcggta tacaaaatct ataaggaggt
      301 gtaccatttc atggcaaaag tagaacttaa acaaccaatc gtagacgaaa ttatcaacaa
      361 cgtgaaagat gcagaatctg tagtacttgt taactacagc ggtcttacag ttgaacagga
      421 tactgcttta cgtaaggaat taagagaagc tggtgttgta tacaaagtat acaaaaacac
      481 aatgatgcat cgtgcattcg aaggtactca gtgcgaagaa cttatccagc acttacatgg
      541 aacaaacgct atagcaatct ctgctacaga cgcaactgct cctgcaagaa tccttgataa
      601 attcgcaaag aaagcaccgg ctttagaatt agttgcaggt atcgttgaag gaaactacaa
      661 cgatcaggca ggtattcagg cactggctgg aatcccgtcc agagaagaac ttctcggcaa
      721 actgcttgga agcatccagt ctccgattac aaacttcgct cgtgtgctta atcagatcgc
      781 tgagaaaaac ggctctgaag cagcagctga ataatttttg atgccagagg cataaaacga
      841 atctagtaac tattcgaaaa tcaccatcca cgaggatgct gaagagttac cgaattaaat
      901 aatattatat ggagggaatt taaaatggca aaattaacaa cagaagaatt tattgcagca
      961 atcaaagaat tatctgtatt agaattaaac gacttagtaa aagcttgtga agaagagttt
     1021 ggtgtatccg cagcagcagg tgttgtagta gcagcagctg gagcagcaga agcagcagaa
     1081 gagaaagatg agttcgatgt agaacttgca gaagttggac caaacaaagt taaagttatc
     1141 aaagttgttc gtgaagtaac tggcttaggt cttaaagaag ctaaagaaat ggttgacgga
     1201 gctcctaagg ttgttaaaga aggcgcatcc aaagcagaag ctgaagatat caaaacaaaa
     1261 cttgaagctg aaggcgctaa ggttaacctt aaataattat cttcctatta tatatagaag
     1321 atttgcaagc aggaatgcca ctggtattcc tgcttttttt acagagccgg aggattacag
     1381 actatgatga aaagaaaaac ggcagtcata tttggtatca tcattgcagc tcagctttgc
     1441 ctgattccat atgcaggtgt aaaagtacag gcggctgaac tggaagaagg acagatgttt
     1501 gaagattcat cagaagattc agagactttt ggagatgtgt caataccgga tacgcagtcg
     1561 gcagagaaaa ctgaagagaa tgaatttggg gatgacgacg gtttcagtga tggagacgtt
     1621 gaaacttttt ctgcggagga tgcggatcag tcctgcgaaa atatgcagga acttgtgctg
     1681 aatgtgcagg atggagagga tattactgta aagttaaata cactgctggc acaggccagg
     1741 gacagggcaa cggatgaaaa gcagtgcaaa gttatcgtgc cgcccggaaa ttatacactt
     1801 acgggaactt tgcatatgta cagcaatatc tatctatatg cagagggtgc cacgataaca
     1861 aagacttcac cgcgaaaaga aatcctgctc cgtcttggcg atacacagaa atctgcaggc
     1921 ggatacgagg gataccgtaa tataactatc gatggcggaa cctgggattc aaactatgaa
     1981 tgtgtggaag acaagggagg acctggaggg tttgtaggtt ttaggatcgg tcatgctacg
     2041 aatgttacag tgaagaatgt tacatttctc aataacctta aatcgcattt tctggaactt
     2101 gcaggagtga agaatgcgga aattacagga tgtacgttca ggggctactg gaaagagttt
     2161 acaggtggcg gacaggaatg tatccagctg gatgcctgta tgccgagaat tttcccaggg
     2221 tatcttccat atgacggaag tgtctgtgag aacattgtga taaaggataa tacctttgaa
     2281 gatgtatttg ccggtattgg aagccacagc atgatgtttg ataaaccgta taaaaatatc
     2341 acgatcagta acaaccggtt taataatctg aaaaagagag caatatggtg cctgaattat
     2401 caggatacgg ttgtgacggg aaatacca
//
LOCUS       DBMDXG010000339         2412 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9345_length_2412_cov_1.716140, whole genome shotgun sequence.
ACCESSION   DBMDXG010000339 DBMDXG010000000
VERSION     DBMDXG010000339.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9345_length_2412_cov_1.716140"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="ACZPD3_08755"
     CDS             complement(<1..131)
                     /locus_tag="ACZPD3_08755"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1416111.1"
                     /translation="MCVTICGRFCPDEGVVAGYDNRIRVKYTAKWGVQIAGMIFQTGS
                     "
     gene            69..383
                     /locus_tag="ACZPD3_08760"
                     /pseudo
     CDS             69..383
                     /locus_tag="ACZPD3_08760"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
     gene            347..1537
                     /locus_tag="ACZPD3_08765"
     CDS             347..1537
                     /locus_tag="ACZPD3_08765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR1416112.1"
                     /translation="MAALFLAPLYILINAYVVRWMIRWMGACHRLFQTMAFRASFIGV
                     YIILATALLTGFLIKKPANLHRILKHTGNYFLGTFIYILLVIAVVDFGRLILKYIFHA
                     PFIGHRSTFVITGLICTILIISLSVYGILHVTHVKTTPYEINVEKTVDGMDSLKIVLL
                     ADTHFGYSMGTAQAKRIVKKINTENPDVVCIAGDIFDNEYDAIYKPDKLKEILKGIKS
                     KYGVYACWGNHDLNEPILAGFTFNGTEDNYNDPRMEEFLTDAGIHLLDDEAVLIDNKF
                     YIVGRRDASRCEKVEGSRATPSQLTESLDQNKPIIFIDHQPKELDETAAAGVDLDLCG
                     HTHDGQIFPGNLIIHLFWENSCGYLRKGTMHNIVTSGAGIWGPNMRVGTNSEICPITV
                     HFNN"
     gene            complement(1739..2164)
                     /locus_tag="ACZPD3_08770"
     CDS             complement(1739..2164)
                     /locus_tag="ACZPD3_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416113.1"
                     /translation="MRLLKKYFNRENKFSLKERFNMVMTVVTCFFMGVMSENGRGMQG
                     IKGLIDGIGGLAVRMNGWLSVVTGWELGAHEAYEIYEKGIVGRFFQGKMLEGIDVVKR
                     GQALVERAGLRGRRGNRIRDVSHEKGLPCMERWGCRGSP"
     gene            complement(2291..>2412)
                     /locus_tag="ACZPD3_08775"
     CDS             complement(2291..>2412)
                     /locus_tag="ACZPD3_08775"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1416114.1"
                     /translation="CDAHLAESLFQTRSRTCTIMKSAQFAEEFFQTYSGGCLL"
BASE COUNT          669 a          651 c          416 g          676 t
ORIGIN      
        1 gagcctgttt gaaaaatcat tcctgcaatc tgcacgcccc actttgcggt atatttcacc
       61 cgaattcggt tgtcgtagcc cgctacgacg ccctcatccg ggcaaaatct cccacaaatt
      121 gtgacgcaca tcttgcagaa agcctttttc aaacacgctc taggtttcaa gaatctttct
      181 cgctttgccg cataagcaac agctctcctg tctatctcat ttctccttat tatatgcaca
      241 attgcaaaaa cttattaacc gtagtatact gtacccattg ttgcgcagac acggtaacaa
      301 agcgtgtaac tgctaatttt caaagtaaat ataacggagg tttttcatgg ccgcactctt
      361 tctcgcaccc ctatacatcc tgatcaatgc atacgttgtc cgttggatga tacgatggat
      421 gggcgcctgt cacagacttt ttcagacaat ggcatttcgc gcttccttta taggagttta
      481 cataatatta gcaacagccc tgctcaccgg ctttctcatc aaaaagccgg ccaacctgca
      541 ccgtattctc aagcatacag gtaactattt tctcggaact ttcatctata ttctgctggt
      601 aatcgctgtc gttgattttg gccgtctgat actgaaatac atattccatg ccccgtttat
      661 cgggcaccgc agcactttcg tcatcacagg cctgatctgc accatactga tcatcagcct
      721 cagtgtttac ggaatccttc atgtaaccca tgtgaaaact acaccttatg agataaacgt
      781 tgaaaaaaca gttgacggca tggattccct gaaaatagtc cttctcgctg atacccattt
      841 cggctacagc atgggaaccg cccaggcaaa acgaattgtg aaaaagatca atactgaaaa
      901 tccagatgta gtctgtatcg caggtgatat tttcgataat gaatatgacg ccatttacaa
      961 accagataaa ttaaaagaaa ttttaaaagg aattaagtcc aaatatggag tttatgcctg
     1021 ctggggcaat cacgacctga acgaaccaat tctggcaggt ttcaccttca acggaacaga
     1081 agataactac aatgatccca gaatggaaga atttcttaca gacgcaggaa tccacctgct
     1141 agacgatgaa gctgttctga tcgacaacaa attttatatt gtcggccgcc gggatgcctc
     1201 ccgctgtgaa aaagtagaag gctccagagc cactccatcc cagcttaccg aatcgctgga
     1261 tcagaacaaa ccaattattt ttattgacca tcagccaaaa gaattagacg aaacagctgc
     1321 tgccggtgtt gaccttgacc tttgtggtca cacccatgac ggtcagattt tccccggaaa
     1381 cctgattatc catctattct gggaaaattc ctgtggctac cttcgaaaag gaactatgca
     1441 caacatcgtc acttccggag ccggaatctg gggaccgaac atgagagtcg gcaccaacag
     1501 cgaaatctgt ccgattacag ttcattttaa caattaatgt aactattccg cagatgctat
     1561 accgcgagat gttttcgtga acatagttca cagttaccaa ttaatttttc aaatattgcc
     1621 ggttcagatt ttaagccatt ccaaccatgc ttcactgtat ttatatacag agccaaccat
     1681 tacatatcca cctgacaata taggtttcat agttacatta aatcaaccga ttatgcctct
     1741 acggactgcc tctgcacccc catcgttcca tgcaaggcag tcctttctca tgtgaaacat
     1801 cccgtatccg atttcccctt cttcctcgca gcccagccct ttccactaaa gcctgtccac
     1861 gcttcaccac atctattcct tccagcatct ttccctggaa aaatcttccc actatacctt
     1921 tttcatatat ctcataagct tcatgcgcgc ccagctccca gccagtcacc acactcagcc
     1981 atccattcat ccttacagcc agtccgccga ttccatcaat cagtcccttt attccctgca
     2041 taccccgtcc attttcactc atcactccca tgaaaaaaca agtcaccacg gtcatcacca
     2101 tattgaaacg ttctttcaaa ctgaatttat tttcccgatt gaaatatttt ttcaataatc
     2161 tcataaaggc cctccttctt gtcatgcctg tccaaaatcc aaaagcttat caccgctgct
     2221 gtctcctaat gcaattaact ttgtaccatt tcgggcactt tttctgcctc cggcagactg
     2281 cacggacagc tcacagcaaa cagccgccag aatatgtttg aaaaaactcc tctgcgaact
     2341 gcgcactttt catgattgtg caggttctag agcgtgtttg aaaaaggctt tctgcaagat
     2401 gtgcgtcaca at
//
LOCUS       DBMDXG010000340         2407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9371_length_2407_cov_1.487858, whole genome shotgun sequence.
ACCESSION   DBMDXG010000340 DBMDXG010000000
VERSION     DBMDXG010000340.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9371_length_2407_cov_1.487858"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="ACZPD3_08780"
     CDS             complement(<1..299)
                     /locus_tag="ACZPD3_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-binding protein"
                     /protein_id="HIR1416115.1"
                     /translation="MRKKVLSAVLAATMVAGMMGNVVSVSAEEKYDLTLYSVNTTDPD
                     FDDWLANVEEATGLNINVIAAPTDTDTRQQKITTILSTGDSSVDIVEINDEMSAA"
     gene            573..>2407
                     /locus_tag="ACZPD3_08785"
     CDS             573..>2407
                     /locus_tag="ACZPD3_08785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1416116.1"
                     /translation="MKLNLKKINYHKWIPSRFSSFQAKLLIAFLVATLLPLTLVALVS
                     YHVSYNLARDRITNSALMSDEQLIFQLNSRLNQTENVADTIQYQMYAFEHSSPDQVGT
                     MQALTSMRSNISLYKSTFDFFHIYVFLKPEQTGADEGIYFFSTDKLFNYGISESELHS
                     IGSSSLWRLKKNTTLPRVISASKRSANTLLCFRALQDKSSNVLEYAYCIALDCDEFSR
                     YLQTSASDTAISSYILTPQGQIAAHSDSSRNQTWISDSIKQMFLENTNSFFKDNDIYY
                     NCRTLDNGWLHITEIPENYIKSNTQVLIKTLLITVIISLPLTIFIVILFSRKLTSRIA
                     ALSYAMESFHLGHDPEHLSIITLPHPADASNYDEIDNLGITFEDMQHTIAQNLKSIVS
                     LSINEERLKYQLLQSQINPHFLYNILGTIRTCQALGKLDIADQMLTNLTAFYRLTLRK
                     SKELIPIKDELEIARLYLEMEKLCHKDNLNWEINAEDGIENFTICKFTLQPFLENSIL
                     HGLSADTPEVFISIQVLYGDDTVVISIEDNGAGMSPETLEQLRYAIDNKVINYEKHFG
                     ISNVSARISNPLYGNGSVRIDSHPGNGTYVTIEFQQMEDTDEENN"
BASE COUNT          728 a          541 c          401 g          737 t
ORIGIN      
        1 aatgcagcac tcatttcatc gttgatttcg acaatgtcta cggaagaatc tcctgtagaa
       61 agaatcgttg taatcttctg ctgacgtgtg tctgtatctg tcggagctgc aataacattg
      121 atatttaaac ccgttgcctc ctctacattt gcaagccagt catcaaaatc cgggtcagta
      181 gtgttaacgc tgtataaagt aagatcatat ttctcctctg ctgatacact tacaacattc
      241 cccatcattc ctgccaccat tgttgcagct aaaactgcac ttaacacttt tttcctcata
      301 ttctctcctt ttccgaacag ttctctcgca tcaactgttc tgtgttattt ggtaattaaa
      361 gaataacaaa aacactcttt ttactaaata caatttcttt tgattgattt atattatttt
      421 tcgttctatt gtttttttca ttctgatttt ttataataca gatagcagat gaacactgtg
      481 ttgcatcttt acagaattgc tgtataatat ggcaatcttc aaaaagatac ctgataaaat
      541 caaacctatt catataagga gtattatgtg taatgaaact caacttgaaa aaaataaatt
      601 atcataaatg gattccttcc agattttcct catttcaggc aaaactcctg attgcctttc
      661 tggttgcaac cctgcttcct ctgacacttg ttgcactggt cagttaccat gtttcctata
      721 atcttgccag agaccgaatc acaaactctg cattgatgtc tgatgaacag cttatttttc
      781 agcttaacag ccgtctgaac cagaccgaaa acgttgctga caccatccag tatcagatgt
      841 acgcttttga acactcttcc ccagatcagg ttggcaccat gcaggcatta acatccatga
      901 gaagcaatat ttcattatat aaatctacat ttgatttctt tcatatatat gtttttttaa
      961 aaccggaaca aaccggagct gatgagggta tatatttctt ttccacagat aaacttttca
     1021 attatggaat cagcgaatct gaattacatt ccataggttc ttcttccctg tggcggttaa
     1081 agaaaaatac caccttaccc agagttatat ctgcttccaa aagaagcgca aatacactgc
     1141 tgtgttttcg tgctcttcag gacaaatcca gcaatgttct cgaatatgct tattgtattg
     1201 cacttgactg cgatgaattt tcccggtatc ttcagacttc tgcttctgat accgcaattt
     1261 ccagttacat tctgacacca cagggacaaa ttgcagctca cagtgattct tcccgtaatc
     1321 agacctggat ttcagattca ataaaacaga tgtttctgga aaataccaat tccttcttta
     1381 aagacaatga catctactat aactgccgca ctctggataa cggttggctg catattacgg
     1441 aaatcccgga aaactatatc aaaagcaata cacaggtact gatcaaaaca ctgttgatca
     1501 ctgtaattat ttctctgccg ctgactattt ttattgtaat ccttttttcc aggaaactga
     1561 caagcagaat tgccgcactg tcctatgcca tggaatcctt tcatctcgga catgatccgg
     1621 agcacctttc catcatcaca ctcccgcatc cggccgatgc ttccaattat gatgaaatag
     1681 acaatctggg gattaccttt gaagatatgc agcacacaat tgcccagaat ctgaaatcaa
     1741 ttgtcagtct ctccattaat gaagaacggc tgaaatatca gttattacaa tcacagatca
     1801 atccacattt tctctacaat atccttggaa ccatccgaac ctgccaggca cttggcaaat
     1861 tggatattgc agatcagatg ctgacaaatc tgactgcctt ttaccgactg acgctgagaa
     1921 aatcaaaaga actgataccg atcaaagacg aactggaaat tgcccgcctg tatctggaaa
     1981 tggagaaact ctgccataaa gataatctta actgggagat caatgccgaa gacggaatag
     2041 aaaatttcac gatctgcaaa tttacattac agccattcct ggagaattct atcctgcacg
     2101 gtctttcagc cgatacccct gaagttttta tttccattca ggtactctac ggtgatgata
     2161 ccgtagtgat atccattgaa gacaatggag ccggaatgtc tccggagaca ctggaacagc
     2221 tccgatatgc catagacaac aaagtcataa attacgaaaa acattttgga atttcaaatg
     2281 taagcgcccg catttccaat cctttgtacg gtaacggctc tgtgcgtatt gacagccatc
     2341 ccggaaacgg tacctatgtc acaattgaat ttcaacaaat ggaggataca gatgaggaaa
     2401 ataatga
//
LOCUS       DBMDXG010000341         2376 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9498_length_2376_cov_1.405275, whole genome shotgun sequence.
ACCESSION   DBMDXG010000341 DBMDXG010000000
VERSION     DBMDXG010000341.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9498_length_2376_cov_1.405275"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="ACZPD3_08790"
     CDS             complement(<1..1045)
                     /locus_tag="ACZPD3_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR1416117.1"
                     /translation="MAKWVYMFTEGNANMRNLLGGKGANLAEMTNLGLPVPQGFTITT
                     EACTQYYEDGRQINDEIMAQIMEAITKMEGVTGKKFGDKENPLLVSVRSGARASMPGM
                     MDTILNLGLNEDVVNVLAEKSGNARWAWDCYRRFIQMYSDVVMEVGKKYFEELIDEMK
                     AKRGVKQDVELTAEDLHELAEQFKAEYKAKIGADFPTDPKEQLMGAIKAVFRSWDNPR
                     ANVYRRDNDIPYSWGTAVNVQMMAFGNMGDDCGTGVAFTRDPATGANGLFGEFLTNAQ
                     GEDVVAGVRTPMHISEMEQKFPEAFVQFKQVCETLEKHYRDMQDMEFTVEHGKLYMLQ
                     TRNGKRTAKAALKI"
     gene            complement(1402..>2376)
                     /locus_tag="ACZPD3_08795"
     CDS             complement(1402..>2376)
                     /locus_tag="ACZPD3_08795"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR1416118.1"
                     /translation="EKIFEILNKLDIYAFIYIGGNDSMDTIKKLSDYAILTGQTQKFL
                     GVPKTIDNDLALTDHTPGFGSAAKYIGASTKEVIRDALGLTYKKNMITIMEIMGRNAG
                     WLTGATALAKSEDCDGPDLIYLPEVPFDVEKFLAKVKDLLNKKASIVIAVSEGIKLAD
                     GRYVCELGSVGDYVDAFGHKQLQGTATYLANFLAAECGCKTRAVELSTLQRSASHMAS
                     RVDIDEAFMVGGAAVKAADEGDTGKMVVIDRVSDDPYMAATGIYDVHRIANEEKLVPR
                     EWMNKDATNVTKDFVDYIKPLIQGDYQPIMVNGMPRHLVLNMKKGRKK"
BASE COUNT          608 a          559 c          427 g          782 t
ORIGIN      
        1 caattttcag agctgccttt gcagttctct taccgttacg tgtctgaagc atatacagtt
       61 taccatgctc tacagtaaac tccatatcct gcatgtctct gtagtgtttt tcaagtgttt
      121 cgcaaacctg tttgaactgt acgaatgctt ccgggaattt ctgttccatt tcggagatgt
      181 gcataggtgt acgaacaccg gcaacaacgt cttcaccctg agcatttgta aggaattcac
      241 caaacagacc gttagctcct gtagcagggt cacgtgtgaa ggcaacacct gtaccacagt
      301 catcacccat gttaccgaat gccatcatct gtacgttaac agctgtaccc caggaatacg
      361 ggatatcgtt gtcacgacga tatacgttcg cacgtgggtt gtcccaggaa cggaatacgg
      421 ctttgatagc acccattaac tgttccttag gatcagttgg gaaatcagca ccgatttttg
      481 ctttatattc agctttgaac tgttcagcta attcatgaag gtcttctgca gtaagttcaa
      541 catcctgctt aacacctctt ttagctttca tctcatcgat aagttcttcg aaatatttct
      601 ttccgacttc cataactaca tcagagtaca tctggatgaa tcttctgtag cagtcccatg
      661 cccaacgagc attgccggat ttttcagcaa gtacgttaac aacgtcttca tttaaaccaa
      721 ggttcaggat agtatccatc atacctggca tagaagctct cgcaccagaa cgaacggaaa
      781 caagcagagg attttctttg tcaccgaatt tctttccggt aactccttcc attttggtga
      841 ttgcttccat gatctgagcc atgatctcat cattgatctg tcttccgtct tcatagtact
      901 gagtacaagc ttctgttgtg attgtgaagc cctgaggtac aggaagacca aggtttgtca
      961 tttcagcaag gttggcacct ttaccaccca gaaggtttct catattagcg ttaccttctg
     1021 taaacatgta aacccatttt gccattttac attttcctcc taaattattg ttcttttgta
     1081 actattcagc attctgttgt gaagtaactg aacagccaca cctttcgcac ataagtaaat
     1141 atatgtttct tatacgcaca taattatttt atagaatttg tgtcatttcg tcaaggttat
     1201 tctgctaaaa ttagctaaaa tttttataca ttctgcataa tgtgatttat tttattattc
     1261 ttattattgt taaggttttc tcagtccttt attctggtct ttatctacat ttttctctca
     1321 catctcatat ataacaggaa aaaacgggaa gcaaaacaga taagttgatc tgcccggctg
     1381 cccgtttata atttttatca attatttctt tctgcctttt ttcatgttca gtaccagatg
     1441 tctcggcata ccatttacca taataggctg ataatctccc tggatcagag gtttaatata
     1501 gtctacaaaa tccttagtta cgtttgtagc atctttattc atccattctc tcggaacaag
     1561 tttctcctca tttgcgattc tgtgtacatc gtagatacct gtagctgcca tgtagggatc
     1621 atcggaaaca cggtcgataa caaccatctt acctgtgtca ccttcatctg cagctttaac
     1681 tgcagcacca cctaccatga atgcttcatc aatatccact ctggatgcca tgtgggaagc
     1741 acttctctgt aatgtggaaa gttcaacagc tcttgtcttg catccgcact ccgctgccag
     1801 gaagtttgcc agataagtgg ctgttccctg aagctgtttg tgtccaaatg catccacata
     1861 gtctcccacg cttccaagct cacatacata tcttccgtct gccagcttga ttccctcgga
     1921 aactgcgatt acaatagatg cttttttatt cagaagatct tttacttttg caaggaattt
     1981 ctcaacatca aaaggcactt ccggcagata gatcaaatcc ggtccgtcgc agtcctcaga
     2041 ttttgccagt gctgtggcac ctgtaagcca tccggcatta cgtcccatga tctccatgat
     2101 cgtaatcata tttttcttat aagtaagtcc cagtgcatcg cggatcactt cttttgtgga
     2161 tgcgccgata tattttgcag cacttccgaa tccgggtgta tggtctgtca gcgccaggtc
     2221 attgtcgatt gttttcggaa caccaaggaa cttctgagtc tgtcctgtga gaatcgcata
     2281 atcagacagt ttcttgatcg tatccatgga atcatttccg ccaatgtaga taaacgcata
     2341 gatatccagt ttatttaaga tctcaaaaat tttctc
//
LOCUS       DBMDXG010000342         2370 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9519_length_2370_cov_1.281622, whole genome shotgun sequence.
ACCESSION   DBMDXG010000342 DBMDXG010000000
VERSION     DBMDXG010000342.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9519_length_2370_cov_1.281622"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            53..1597
                     /locus_tag="ACZPD3_08800"
     CDS             53..1597
                     /locus_tag="ACZPD3_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR1416119.1"
                     /translation="MLIRNILEESVQKFGEVKAVKWLNRKEVLEKSYSEMMKNVISTR
                     KGLLAEGFEGKHIVLIGTSSVEWIESYLGIITGCATAVPLDAALPCEELIDLINRSDS
                     EALFLSPKHRPYLEAFLANCPKLQKVWMLQEEVEDLPSGVYSINELRDMGKSAAEDAS
                     CPDAEAIATIIFTSGTTGKSKGVMLTQNNLASNVEAVKISAEPGTAMLSVLPIHHAFC
                     LVMDWLKGFSQGATICINDSLLHMVRNMSIFKPDIMLMVPMMIETIYKRLAAADPEIP
                     KAVLAEKVFGGKLQTIFTGGAHLDPYYIDRFAEYGVQILEGYGMSECSPVISNNTPEN
                     HKPGSIGRPLENVEIKFENGEILVKGTSVMKGYYQMPEETAETLKDGWLHTGDKGYMD
                     EDGYLFINGRVKNLIILSNGENVSPEELENKLALNPLVAEVIVTGEENGLTARIYPEQ
                     AVVEAKALDTEMIRLQLQAFLDEYNKNQPTYRRITGLVIRKNPFIRSTTKKIRRQDVL
                     IDEPQA"
     gene            complement(1557..1691)
                     /locus_tag="ACZPD3_08805"
     CDS             complement(1557..1691)
                     /locus_tag="ACZPD3_08805"
                     /inference="COORDINATES: protein motif:HMM:NF042606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6783 domain-containing protein"
                     /protein_id="HIR1416120.1"
                     /translation="MPAKWGVQIAGMLFQTGIRKTRLSNSLRRVFLMPVAHQSGHPVS
                     "
     gene            complement(1707..2285)
                     /locus_tag="ACZPD3_08810"
     CDS             complement(1707..2285)
                     /locus_tag="ACZPD3_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1416121.1"
                     /translation="MQNPVPDERYSVADEAILGAFFKLLKKKTPDQITVSDVTKTAGI
                     ARSTFYNHYQDIPSLVSAVEEKTIQDVFSIMEKFHPENDRDICQSYFLTLCRYTMENP
                     FLSSLLSTPHGNDLFEKMLTMLHHYVTATTSSARPGRHTKEEISYVITCAIGSTIGVL
                     HKWTRDHFDLAPEVIAEILTQVFLTGMLPLLS"
BASE COUNT          724 a          451 c          588 g          607 t
ORIGIN      
        1 acagtaagac ttaacagagt gtcagttcac agagcggtga aggaggaaaa atatgctgat
       61 caggaatata ttagaagaaa gtgtacagaa atttggcgaa gtcaaagcag taaaatggct
      121 taatcgaaaa gaagttctgg agaaaagcta cagcgaaatg atgaaaaatg tgatttctac
      181 cagaaaagga cttcttgcag aagggtttga gggaaaacat attgtattaa tcggaaccag
      241 ttctgtagaa tggatcgaaa gctatctggg gattattaca ggatgcgcga cagcggtgcc
      301 gctggatgct gcactcccgt gtgaagaact gattgacctg ataaatcgct ccgattccga
      361 agcactcttc ttaagtccga aacacagacc atatctggaa gcatttcttg caaactgtcc
      421 caagcttcag aaagtatgga tgctccagga agaggtggag gatctgcctt caggagtgta
      481 cagtatcaat gagctgagag atatgggaaa gagtgcagca gaagatgctt cctgtccgga
      541 tgcagaagct attgcgacta ttatctttac ttccggaacc acaggcaaga gcaaaggggt
      601 tatgctgaca cagaataatc tggcatccaa cgttgaggca gttaagattt cagcagagcc
      661 tggaactgcc atgctcagtg tacttccaat ccatcatgca ttctgccttg tcatggactg
      721 gctgaaagga ttttctcagg gggcaactat ctgtatcaat gactccctgc ttcatatggt
      781 cagaaatatg agcattttta aaccagatat catgctcatg gttccgatga tgattgaaac
      841 aatttataag agactggcgg ctgctgatcc ggaaatcccg aaggctgttc tcgcagaaaa
      901 agtattcggt ggaaaactgc agacgatttt taccggcggg gcacatctgg atccgtacta
      961 tattgaccgt tttgcagaat atggcgtcca gatactggaa ggatatggca tgtcggaatg
     1021 ttctcctgtt atcagtaata atacaccgga aaatcataag ccgggttcta tcggaagacc
     1081 acttgagaat gtggagataa agtttgaaaa tggggagatt ctggtaaagg gcacaagtgt
     1141 tatgaaaggg tactatcaga tgccggagga gacagcggaa acactgaagg atggctggct
     1201 gcatacaggc gacaagggct atatggatga agatggatac ctctttatta atggtcgtgt
     1261 gaaaaatctg atcattctct ccaatgggga aaatgtatca ccggaagaac ttgagaacaa
     1321 gcttgcgctg aacccgcttg ttgcggaagt gattgtgaca ggtgaagaga atggtctgac
     1381 agcacgcatc tatccggaac aggcagttgt ggaggcaaag gcactggata ctgagatgat
     1441 tcgtttacag cttcaggcat ttctggatga atacaataaa aatcagccga cctaccgtcg
     1501 tattacaggt ctggtgatcc ggaaaaatcc gtttatacgc agtacaacaa agaaaatcag
     1561 gagacaggat gtcctgattg atgagccaca ggcataagaa aaacccgtct gagtgaattg
     1621 cttagacggg tttttcttat gcctgtttga aagagcattc ccgcaatctg cacgccccac
     1681 tttgcgggaa tgcttttaca aacacgtcag gataaaagtg gcagcatgcc agtcaggaaa
     1741 acctgagtca gaatctctgc aatgacttct ggtgcaagat caaaatgatc ccgggtccat
     1801 ttgtgaagaa caccgatcgt gctgccgatc gcacaggtga tcacataaga tatttcctct
     1861 tttgtgtgtc tgccaggtct ggcactggag gtagtagcgg tgacatagtg atgaagcata
     1921 gtcagcattt tttcaaagag atcattccca tggggggtgc tcagaagact ggaaaggaaa
     1981 ggattttcca ttgtatagcg gcaaagtgtc agaaaatagg actgacagat atcgcggtca
     2041 ttttcaggat gaaatttttc cattatagaa aaaacatcct ggatcgtctt ttcctcaaca
     2101 gcggatacaa gagaaggaat gtcttgataa tgattataaa aagtgcttct cgcgatacca
     2161 gctgtcttag tgacatcgga tactgtgatc tgatcaggag tttttttctt tagaagttta
     2221 aaaaaagcac ccaggatggc ttcatccgcc acagaatatc tttcgtcagg aactggattt
     2281 tgcatagtcg cttccctccc aatattttat caataaatat aagaatatga gtggcaaaag
     2341 accttttgac tctcatatca tgtatatatg
//
LOCUS       DBMDXG010000343         2368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9529_length_2368_cov_1.195412, whole genome shotgun sequence.
ACCESSION   DBMDXG010000343 DBMDXG010000000
VERSION     DBMDXG010000343.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9529_length_2368_cov_1.195412"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="ACZPD3_08815"
     CDS             <1..191
                     /locus_tag="ACZPD3_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416122.1"
                     /translation="VAVAMDTVMAIDMVITTVTAQDTTKVPTENRSATMAKAQAAVSF
                     FLYLFRLKEDVRQTLRIF"
     gene            complement(336..1592)
                     /locus_tag="ACZPD3_08820"
     CDS             complement(336..1592)
                     /locus_tag="ACZPD3_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR1416123.1"
                     /translation="MYLQQYEVSMTILDYFYPRLKNYIDFDFTDQLSPPRLKKDGNYY
                     ATYVSEIRQEHAVLSPDRCSVVLKEIPASLTEIYRKISSRWSPYIETVYGVLSENGHS
                     LAINEFIQKPACLSYPTKELLEMRSLTLEDFILKMRTLSEKEALIFLLQLCEALENIS
                     ELSLVHGDVSPQNILLTDVLPLNSQKQQPTSSTGKSITELCRNVAVKLIDFDIAREEK
                     GSHHMVTTVAGTNPYAAPEILDYQTPTDRVDIYSLGCVFAFMLTGKSPKQMSREEFQS
                     SLSKSVRKIINKCTADYSRRYKDVTHLKKDISALLSTSTMPVPEFFHILPGLRSGKPL
                     EICIFSLIYCALFHLLIFLICPSPSLAWILPAVAVSVILTTDVFHLGRHFAFYQLCLE
                     QQPEWCYLFKFLIGFVLPFTVLRMIS"
     gene            complement(1750..2169)
                     /locus_tag="ACZPD3_08825"
     CDS             complement(1750..2169)
                     /locus_tag="ACZPD3_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416124.1"
                     /translation="MTKKLSTNDIFAKIEKGEPVDVKEVEFVSPDTMRDKLYAKITEM
                     PSSINHFADLCGIYPSHLHEFLKGTKNLGRDKLIIVCIVLKLNLRETREVLKCTLQSD
                     FYPKNQRDFIIMSGIKAHRSVDDINELLMNNGLSILS"
BASE COUNT          722 a          427 c          491 g          728 t
ORIGIN      
        1 aagtggcggt cgctatggat accgttatgg ctatcgatat ggttatcact acggttacgg
       61 ctcaggatac cactaaggtt ccgacggaaa acagatcggc tacaatggca aaagcacagg
      121 cagcggtaag ctttttcctg tatttgtttc gtttaaagga agatgtccgg cagactctga
      181 gaatattctg accgtattgt gtggagttga tttctgtttc tttttcatat ccggaggatt
      241 taaatgtctc cagtatctgc tgtcttttca gaatttctat aacagatgaa tccataatgt
      301 ataacctctt tgcttatagc aattctataa gttttttatg atatcattct caaaacagta
      361 aacggcagca caaatccgat cagaaactta aaaagataac accattcagg ctgctgttca
      421 aggcaaagct gataaaaggc aaagtgtctg ccaagatgaa aaacatcagt agtcaggatt
      481 acagaaactg ctactgcagg aaggatccag gcaagtgaag gtgaggggca gatcaggaat
      541 atcagaagat gaaaaagtgc acagtaaatc agggaaaaaa tacatatttc caatggtttt
      601 ccactccgaa gacctggcag tatgtggaaa aattccggaa caggcattgt ggaagtggac
      661 aaaagagcgg aaatgtcttt tttcaaatgt gtaacatcct tatatcttct ggaataatcg
      721 gcagtacatt tattgattat tttgcggact gatttagaca gactgctttg aaattcttct
      781 ctgctcatct gtttaggtga tttaccggtg agcataaagg caaaaacaca tccgagagaa
      841 tagatatcga ctctgtctgt aggtgtctgg taatccagaa tctccggtgc agcataggga
      901 ttggtacctg ctacggtagt gaccatatga tggctcccct tttcttctct ggcaatatca
      961 aagtctatca gtttcacagc tacattccgg catagttctg tgatactttt tccggtggaa
     1021 gatgtgggct gctgcttttg ggaattcagg ggaaggacat ctgtaagcag gatattctga
     1081 ggagaaacat caccatgtac cagagaaagt tccgaaatat tttccagagc ttcgcaaagc
     1141 tgaagaagga aaataagtgc ttctttttcg gagagtgtac gcattttcag aataaaatcc
     1201 tccagggtga ggcttcgcat ttccaaaagc tcctttgtgg gataactgag gcaggcgggt
     1261 ttctgtataa attcgttgat tgccagagaa tgtccgtttt ctgagagaac accatacaca
     1321 gtttcgatat aaggcgacca tctggaactg atttttctgt aaatttcagt taaggaagca
     1381 ggaatctctt tcaggactac actgcatctg tcaggcgaaa ggacagcgtg ttcctgcctg
     1441 atttcagaaa cataggtagc atagtaattt ccatcttttt ttaatctggg cggagaaagc
     1501 tgatctgtaa aatcaaaatc aatataattt ttaagcctgg gatagaaata atctaaaata
     1561 gtcatagaaa cctcatattg ctgtaaatac atataacgcc tgctctctga gaagaaaaca
     1621 ggcgtgtttt tcggtaagtg atcgtgtcag ctgtaataca ttgtatcact tccggatcaa
     1681 aatataaatg attttctgtg aaaaatatat agcaattccc gttgctgcag gaatacgatg
     1741 agcaataaat caggataaga tagaaagtcc attgttcatg agaagttcat tgatgtcatc
     1801 tacacttcta tgtgctttaa tacctgacat aataataaaa tctctctggt tttttggata
     1861 aaagtcagat tgcagtgtac attttaatac ttctctggtt tccctgagat ttaatttcag
     1921 aacgatacag acgatgatca gtttgtcccg gccgagattc ttggttcctt tcagaaattc
     1981 atgaagatgg gaaggataaa taccacacag atctgcaaaa tgattaatgc tggaaggcat
     2041 ttctgtgatc ttggcatata gtttatccct cattgtatca ggagaaacaa attctacttc
     2101 ttttacatct accggttctc ctttttcaat tttggcaaag atatcatttg tgcttagttt
     2161 cttagtcata atttcccctt taagttactg tatccggcaa taacttcctt aaaattcgtg
     2221 gatatgatgt cagtgctaaa acattcttaa cctgacatta tatatgtact atatatctca
     2281 aagtgaatct ttgtaaagag aaaacgggga attatatact gacagtgtat gaaaacgttg
     2341 ctattcactc tgttctcagt aaaacttt
//
LOCUS       DBMDXG010000344         2365 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9542_length_2365_cov_1.979682, whole genome shotgun sequence.
ACCESSION   DBMDXG010000344 DBMDXG010000000
VERSION     DBMDXG010000344.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9542_length_2365_cov_1.979682"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="ACZPD3_08830"
     CDS             <1..309
                     /locus_tag="ACZPD3_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR1416125.1"
                     /translation="YDNDMIGLVFKNDSKDKQYTMEVYTTDGKLKFKENFNIPYTTIK
                     LSGGNILMYNSSQMCVMNSRGVQKYLGSVDGTIKDFFKIGMNRYLLVLDSGVEIIKLS
                     "
     gene            320..1018
                     /locus_tag="ACZPD3_08835"
     CDS             320..1018
                     /locus_tag="ACZPD3_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014704.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR1416126.1"
                     /translation="MALTWAGVITILFLAAACVRGYRRGLIKELVSLVCVFLSMAIVW
                     FINPYVNEFIRENTSIYEKVQESCREFVGEEYSTWTGSGESQTEFINEMNLPELLRNG
                     LVQNNNSDSYQYLAVTTFSDYIAQYLARMAVNGISFLISLLMSTIMVRSITWMLNLVT
                     RLPVLHGMNKVAGALLGAVKFLIVIWIIFLALTIVCNTKVGEAALQIIKKDCILSFIY
                     DRDILIRIFMSIFY"
     gene            1353..>2365
                     /locus_tag="ACZPD3_08840"
     CDS             1353..>2365
                     /locus_tag="ACZPD3_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_173702612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR1416127.1"
                     /translation="MNNKGNRKHLTFEQRVEIEKALTENKSFAEIGRIIGKDSSTISK
                     EVRLHARVKERPDPGYTNPPCIHRKKCKMTCLCDEQCGTMCKICKKQNMRCIDVCPAY
                     ETAECEKLKKPPYVCNGCAKKSHCLMPRMFYSSKYAHDEYRSVLVDSRVGINQTPESI
                     QSMNDLLVPLIRDKHQSIGHIYATHAEELGCSRRTLYSYINDCVFDIRNGDLRRSVRY
                     KKRRKPTKAGSKDRSYRQGHNYEDFQDYMKEHPDTNVVEMDCVEGKKGESRSILTFTF
                     RNCNLMLMFLLEYQDQECVLEVFVWLETVLGQEAFKKLFPVILTDGGSEFSARKEMEE
                     FCDG"
BASE COUNT          734 a          395 c          547 g          689 t
ORIGIN      
        1 tatgataatg atatgattgg tcttgtgttt aagaatgaca gtaaagacaa acagtataca
       61 atggaagttt atactacaga tggcaaattg aaattcaaag aaaacttcaa tattccatat
      121 acgacgatta aattaagcgg tggaaatatt ctgatgtaca acagttccca gatgtgtgta
      181 atgaacagca ggggagtaca gaaatatctt ggcagtgtgg atggaacgat caaagatttc
      241 ttcaaaatcg gaatgaacag atatcttctg gtattggaca gcggtgtaga gataataaaa
      301 cttagttagg aggatgaaaa tggctttaac gtgggccgga gtcattacga tactttttct
      361 ggcggctgca tgtgtcagag gttataggcg gggactgata aaagaactgg tttcgcttgt
      421 atgtgttttt ctgtcgatgg cgatcgtgtg gtttataaat ccttatgtaa atgagtttat
      481 tcgggaaaat acatcaattt atgaaaaagt acaggaaagc tgccgggagt ttgtaggaga
      541 ggaatacagc acatggacag gcagtggaga aagccagact gaatttataa atgaaatgaa
      601 tctcccggaa ctgctgagga atggactggt tcagaataat aattctgaca gttaccagta
      661 tttggctgta actacatttt cggattatat tgctcaatat ctggcgagaa tggcagtgaa
      721 tggaatttct ttcctgattt ctctgctgat gtcgacgatt atggtgagga gtatcacatg
      781 gatgctgaat cttgtgacga gattacctgt gttgcatggc atgaacaagg tagcaggagc
      841 gttgctgggg gcagttaaat tccttatagt aatatggatt atctttctgg ctctcaccat
      901 tgtgtgcaat acaaaggttg gtgaggcggc ccttcagatc attaagaaag attgcatcct
      961 gagtttcata tatgacagag atatcctgat caggatattt atgagtatct tctattaaaa
     1021 aggtttggta tctgtggacc gtaggtatcg gatccaaatg tcttcctgcg caaatgcgaa
     1081 cgatttttga ataattgcgc gtcgcattct cgttacttag tgatgagtta gacgcgcaaa
     1141 ttttttaccc aaaggaaaac atatgtttac ttttagcgct atatgtgcta aaatatagcc
     1201 atggacttaa atgtattttt aacttccagt tggcaagagt aaattccagt ctcatagaaa
     1261 ttacttctgc acatatatat tgtcattgat gaccattgct atgtatactg tacttgcaac
     1321 tgattgcctg cctgtgcaga ttggagataa atatgaataa taaaggcaat cggaaacatt
     1381 taacatttga gcagcgtgtt gagattgaaa aagctcttac tgagaataaa agctttgctg
     1441 aaattggaag aatcataggc aaagattcct caacaatatc taaggaagta cgtcttcatg
     1501 cacgtgtcaa agagcgtccg gatccgggtt ataccaatcc tccctgcatt catcgaaaaa
     1561 aatgtaaaat gacctgtctc tgtgatgagc aatgtggaac tatgtgtaaa atctgtaaaa
     1621 agcaaaatat gcgttgtata gatgtatgtc ctgcatatga aacggcggaa tgtgagaagc
     1681 ttaaaaagcc gccatatgta tgtaatggat gcgctaaaaa aagccattgt ctcatgccaa
     1741 gaatgtttta ttcttccaag tatgctcatg atgaatatcg cagtgtgctt gttgatagca
     1801 gagttggtat caatcagact cccgaaagta ttcagtccat gaacgattta ctggtcccac
     1861 tgatcaggga taaacaccag tccatcggac acatttatgc tacccacgca gaggaattag
     1921 gctgctccag aagaacactt tactcttaca ttaatgattg tgtatttgat atccgcaatg
     1981 gtgatttaag acgttctgtg cgctataaaa agcgcaggaa acctacgaag gcaggatcaa
     2041 aagaccgttc ttaccgccaa ggtcataact acgaagattt tcaggattat atgaaggaac
     2101 atccagatac caatgtcgtg gaaatggact gtgtggaagg taagaaaggc gaaagcagat
     2161 ctatcttgac ctttacattc cgcaactgca atcttatgct catgttcctt ttggaatatc
     2221 aggatcagga atgcgtcctg gaggtctttg tatggctgga aacagttttg ggacaggagg
     2281 cattcaaaaa gctttttcca gtgatcctca cggatggagg ttctgaattc tccgcccgaa
     2341 aagagatgga agaattctgt gacgg
//
LOCUS       DBMDXG010000345         2365 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9545_length_2365_cov_1.472173, whole genome shotgun sequence.
ACCESSION   DBMDXG010000345 DBMDXG010000000
VERSION     DBMDXG010000345.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9545_length_2365_cov_1.472173"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            274..>2365
                     /locus_tag="ACZPD3_08845"
     CDS             274..>2365
                     /locus_tag="ACZPD3_08845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR1416128.1"
                     /translation="MSDIYSTLNPQQQEAVYHTEGPLLILAGAGSGKTRVLTHRIAYL
                     IDKKGINPWNILAITFTNKAASEMRERVDKIVGFGSESVWVSTFHSTCVRILRRYIDR
                     LGYDTRFAIYDTEDQKTLMKEVCRKLNIDTKKTKERSLLEQISHAKDELITPDEMELN
                     AGEDFVKKKVAEVYREYQAALRRNNALDFDDLIVKTVELFQNCGDVLENYQERFRYIM
                     VDEYQDTNTAQFKFISLLASKYENLCVVGDDDQSIYKFRGANIGNILGFEHVFPDAKV
                     IRLEQNYRSTKNILNAANAVIANNTSRKSKTLWTENSEGERIHFRQFMNGYEEAEYVV
                     GEISRAHRENGAKYKDCAVLYRTNAQSRLFEEKCLLANIPYKIVGGVNFYARKEIKDL
                     LCYLKTIDNSRDDLAVRRIINVPKRGIGATTLGRIQDYADKMSVSFYDALRVAEEVPS
                     IGRSLSKIDGFVTFIQSLKSKAESYTVRELLEEVIELTGYVAELQAEDTDESKARIEN
                     IDELISKTESYQEAMEEQGQTATLSGFLEEIALIADIDSVDPDQDYVLLMTLHSAKGL
                     EFPRVFLAGMEDGMFPSYMSIISDDRSDLEEERRLCYVGITRAMENLTLTSARQRMLR
                     GEVQYNKVSRFVREIPRELVDLGQEAQEKKKKIEEMIQADRNLAKMKMAFETKTFKPR
                     EFKVTKAATLDYEVG"
BASE COUNT          762 a          399 c          578 g          626 t
ORIGIN      
        1 attatatagt tttaaatact acttgtaaat gtttttttca ctaattacaa aatttatatc
       61 cgcttgaatt tacaggaatc agttgtaggc gaacagattt ttgtgtataa tcataaagat
      121 atatgcctgt tcaggcacaa aaaatatttc gagagatgta aacggtaacc ggtacatatt
      181 ttgaatctga aagattgttg caggaatcaa cttgttgatc agcatggttg tttgtaaaat
      241 aatgtaccgt acaggaagaa gacaggagaa aatatgagcg atatttatag tacattaaat
      301 cctcagcagc aggaagcggt ttaccataca gaaggtccac tgctaatcct tgcgggagcg
      361 ggttcgggaa agacaagagt acttacccac cgcattgctt acctgataga taaaaaggga
      421 atcaatccct ggaacattct ggctatcaca tttacaaata aagcagccag tgagatgaga
      481 gaacgtgtag ataaaatagt gggatttggt tccgagagcg tatgggtttc cacattccat
      541 tctacctgtg tgcgtattct gcgtcgttat attgaccgtc ttgggtatga taccagattt
      601 gctatttatg acacagaaga tcagaaaact ctgatgaaag aagtatgcag aaaactgaat
      661 attgacacca agaaaacaaa ggaacgttct cttctggaac agatttccca tgcaaaagat
      721 gaactgatca caccggatga gatggaactg aatgcaggtg aggactttgt taaaaagaag
      781 gttgctgagg tatatcggga atatcaggct gctttgcgca gaaataatgc gctggatttt
      841 gatgatctga tcgtaaagac cgtagagctt tttcagaact gcggagatgt tctggaaaat
      901 tatcaggaac gtttcagata tatcatggtg gatgagtatc aggataccaa tactgcacag
      961 tttaaattta taagcctgtt ggcctctaaa tatgaaaacc tctgtgtagt cggtgatgat
     1021 gaccagtcta tctataaatt ccgcggtgca aatattggaa atattcttgg gtttgagcat
     1081 gtttttccag atgcaaaagt tatccgtctg gaacagaatt accgttccac aaagaatatt
     1141 ctgaatgcag ccaatgcggt gatcgcaaat aatacctcac gtaaatcaaa aacactctgg
     1201 actgagaatt cggaggggga acgaatccat ttccgccagt tcatgaacgg atatgaggaa
     1261 gcagagtatg tggtaggcga gatttccaga gctcacagag agaatggtgc gaagtataaa
     1321 gactgtgcag ttctgtacag gaccaatgca cagtcacgac tctttgaaga gaagtgtctt
     1381 cttgcgaata tcccttacaa gattgtaggc ggtgtgaatt tctatgcgcg taaagaaatc
     1441 aaagacctgt tatgttatct gaagacaatt gacaattcca gagatgatct ggcagtccgc
     1501 cgtatcatca atgtaccgaa gcgtggaatc ggagccacaa ctcttggcag aatacaagat
     1561 tatgcggata aaatgagtgt gagtttctat gatgccttgc gtgtggcaga ggaggtcccg
     1621 tctattggaa gaagcctttc aaagattgat ggatttgtaa cctttattca gagtttaaag
     1681 agtaaggcag agtcttatac agtacgtgaa cttctggaag aagtgattga acttaccgga
     1741 tatgtggcag agctgcaggc tgaagataca gatgaatcga aggcaagaat cgagaatatt
     1801 gatgaactta tttccaagac agaatcctat caggaagcaa tggaagaaca gggacagact
     1861 gccacacttt caggttttct cgaggagatt gcactgatcg cagacattga cagcgtggat
     1921 ccggatcagg attatgtgct tcttatgaca ctacatagtg ccaaaggtct ggagtttccg
     1981 agagtatttc tcgcaggaat ggaagatggg atgtttccgt cttatatgtc cattatatca
     2041 gatgacagat ctgatctgga agaagagaga aggctctgtt atgtgggtat cacaagagct
     2101 atggagaatc tgacgctgac aagtgccaga cagcggatgc tccggggaga agtacagtat
     2161 aataaagtat ccagatttgt acgtgaaatt ccgcgggagc ttgtggatct gggacaggaa
     2221 gctcaggaaa agaagaaaaa gattgaagag atgattcagg cagatagaaa ccttgcaaaa
     2281 atgaaaatgg cttttgagac gaaaacattc aagcccagag aatttaaagt gacgaaggct
     2341 gctacactgg attacgaagt gggag
//
LOCUS       DBMDXG010000346         2358 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9578_length_2358_cov_2.261852, whole genome shotgun sequence.
ACCESSION   DBMDXG010000346 DBMDXG010000000
VERSION     DBMDXG010000346.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9578_length_2358_cov_2.261852"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            237..1463
                     /gene="sstT"
                     /locus_tag="ACZPD3_08850"
     CDS             237..1463
                     /gene="sstT"
                     /locus_tag="ACZPD3_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HIR1416129.1"
                     /translation="MKKVWDKWTRIALVKRILVGLILGAILGIAVPQATGIAILGDVF
                     VSALKAIAPLLVFFLVISSLCNAGKSHGGVIKTVIILYMFSTVLAAVIAVFASMAFPV
                     KMTLANAATDTSAPQGIVEVLNNLLLNVVANPVSSLVNANYVGILMWAVLLGLAFRAA
                     DKMTKKVLADVADGISMVVTWIINLAPFGIFGLVFNTVSTNGLDIFTTYGKLLLLLVG
                     CMLFIYFVTNPLLVYWCIRQNPYPLIFHCLKRSALTAFFTRSSAANIPVNMKVCEEMG
                     LDRDTYSVTIPLGATINMDGAAITITVMTMATAFTLGIHVDIPTAIILSLLAALSACG
                     ASGVAGGSLLLIPMACSLFGISDDISMQVVAVGFIIGVVQDSVETALNSSSDLLLSAS
                     AEFRQWRLEGKEIKFK"
     gene            1735..1986
                     /gene="spoIIID"
                     /locus_tag="ACZPD3_08855"
     CDS             1735..1986
                     /gene="spoIIID"
                     /locus_tag="ACZPD3_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HIR1416130.1"
                     /translation="MKDYIEERAVEIANYIIDTKATVRQAAKKFGISKSTVHKDCTDR
                     LSQLNPGLACEVRQVLDVNKQERHIRGGLATREKYLHQH"
     gene            2093..>2358
                     /locus_tag="ACZPD3_08860"
     CDS             2093..>2358
                     /locus_tag="ACZPD3_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylesterase"
                     /protein_id="HIR1416131.1"
                     /translation="MALMEVNFFSKALMRPVTMNVILPVDKVFFGEETEEENKPFKTL
                     YLLHGVMGNYTDWVTGTCIKRWAEEKNLAVVMPSGANMFYMDHP"
BASE COUNT          679 a          421 c          576 g          682 t
ORIGIN      
        1 cgaacggcga gccgtatgtg gagcagtata atgagacgga tcatcaacag gagaaaagaa
       61 cagtagttgt atagtagcgc tttatgaaaa tacagtttgt attacctacg aaatccttgt
      121 aaaaggatat gaatttttgc tgaccgggta gagaaatcct tgcacttttt taaaaattcc
      181 tttataatgt accggattag tgttttaaca aactaaaggg aaagaggatt aaagaaatga
      241 aaaaagtttg ggacaaatgg accaggattg ctcttgtaaa gagaatcctg gttggcctga
      301 ttctgggtgc gattctggga attgctgtac cgcaggcaac aggaatcgca attctgggtg
      361 atgtctttgt aagcgcgtta aaggcaatcg cacctttgct ggtattcttc ctggttatca
      421 gttccctgtg taatgcagga aaatctcacg gaggagttat taagacagtt atcattctct
      481 atatgttcag tacagttctg gcagcagtga ttgcagtatt tgcgagtatg gcatttcctg
      541 taaagatgac acttgcaaat gcagcaacag atacaagtgc accacagggt atcgttgagg
      601 tattaaataa cctgcttctg aacgtagttg ccaacccggt atcttcattg gtaaatgcaa
      661 actatgtagg gatcctgatg tgggctgttc ttcttgggct tgcttttcgt gcagctgata
      721 agatgacaaa gaaggttctg gctgatgttg cagatggtat ttccatggta gtaacctgga
      781 tcattaatct tgcacctttt ggaatttttg gtctggtatt taacacagta tctacaaacg
      841 gtctggatat ctttacgact tatggaaaac tgttgcttct tcttgtagga tgtatgctgt
      901 ttatctattt tgtgaccaat ccgcttctgg tttactggtg tatccgccag aatccatatc
      961 cgctgatttt ccactgcctg aagagaagtg cgctgacagc tttctttaca agaagttccg
     1021 cagctaatat tccggtaaac atgaaagtct gtgaagagat gggactggac agagatactt
     1081 attctgtaac cattccgctt ggtgcaacta ttaatatgga tggtgcggct atcactatta
     1141 cggttatgac aatggcaact gcatttactc ttggtattca tgtagatatt ccgacagcaa
     1201 ttatcctgag tcttcttgca gctctgtcag catgtggcgc ttccggtgta gcaggtggat
     1261 cacttcttct gatcccgatg gcatgctccc tgttcggaat ttcagatgac atttccatgc
     1321 aggttgttgc agtaggattt atcattggcg tagttcagga ttctgtagaa acagctttga
     1381 actcttcttc agatcttctg ttatctgctt ccgcagaatt cagacagtgg agacttgaag
     1441 ggaaagagat taaatttaaa taagattgca atgagcgaaa cggtcgtccg gtatggacgg
     1501 ccgttctttt atgtcatagg aaattactct gtaatgttcc tataacataa aaaagccctt
     1561 cgggcaggat cgcactgcga cggagaggaa ttatgtcgct gaagcgaccc gccgcaggcg
     1621 gagaatcctg caagcaggat tctttcatat aaggcacaac tataaaaatg attgctacat
     1681 attcgggaat ttctgtgcat acactgtaaa aacagcgtct tcggaggctt atccgtgaaa
     1741 gattatatcg aggagcgggc agtggaaatt gcaaattata ttattgatac aaaggcaacg
     1801 gtgcgtcagg cggcgaagaa attcgggatt agtaagagca ctgtacataa ggactgcact
     1861 gaccgtctca gtcagctcaa tccgggactg gcatgtgaag tgagacaggt tctggatgtg
     1921 aacaagcagg agcggcatat cagaggtggg ctcgctacaa gagaaaaata tttacatcag
     1981 cattagagcg taaaagaagc tgctgtcgga tatgacagta gcttttttca ggtgtttttt
     2041 gtataataaa atgctgtaaa ggctttgcaa aaacataagg gaggataaga agatggcatt
     2101 aatggaagtg aacttctttt caaaagcact gatgcgtccg gtaacgatga atgtaatcct
     2161 gccggttgat aaagtgtttt ttggagaaga aacagaagaa gagaacaaac cgtttaagac
     2221 tttgtatctg cttcacggag taatgggaaa ttacacagac tgggtaacag gaacatgtat
     2281 taagcgatgg gcagaagaaa agaatctggc agtagttatg ccttccggag caaatatgtt
     2341 ctatatggat catccgaa
//
LOCUS       DBMDXG010000347         2358 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9579_length_2358_cov_1.571998, whole genome shotgun sequence.
ACCESSION   DBMDXG010000347 DBMDXG010000000
VERSION     DBMDXG010000347.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9579_length_2358_cov_1.571998"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            300..527
                     /locus_tag="ACZPD3_08865"
     CDS             300..527
                     /locus_tag="ACZPD3_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416132.1"
                     /translation="MNIGNIMKITGAWNTFKSNHPKFPAFCQAVSRKGLKEGSIIEIA
                     ITTPEGEKIETNLKVKDSDLELLKQLSDLKM"
     gene            659..1249
                     /locus_tag="ACZPD3_08870"
     CDS             659..1249
                     /locus_tag="ACZPD3_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR1416133.1"
                     /translation="MRKKSHILLARYLADQMQTTASLQSHRKAFCLGSILPDIKPSFI
                     TRRHEFYGTFEDVKNRMKELADIRPDESNQRVYWRRFGEVIHYMADYFTFPHNKTYTG
                     SFSQHNHYEKVLKNRLKECIQQGEAYAYLEPAIRFADFPTLIDYIEATHEKYLNKLRS
                     VEEDIRFILNMCFQVVQGLIQICIGNKNFAGAIQAA"
     gene            1600..>2358
                     /locus_tag="ACZPD3_08875"
     CDS             1600..>2358
                     /locus_tag="ACZPD3_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR1416134.1"
                     /translation="MKNKYLTRFLCLTLMSGMVLSGPASVLAAEDTAAYTADGSDFSD
                     SGDFDSGSEDPAPADPTPAEPTPAPVDPTPADPTPAPVDPTPTPAEPTPAPADPTPTP
                     TDPTPTPANPTPTPSSQITKAAQDVIDRIKELNAQTITLEKKATVQSIRTAYNALSDT
                     EKAQVSNYNLLVQAEATIAQLEAQKNNANTNNPFTSNTSAAQTGTPVYYASNIHAGKD
                     FYLDSLKNNYNLTFSDDFASVMDEIEKEYKEKNKV"
BASE COUNT          775 a          486 c          519 g          578 t
ORIGIN      
        1 aatctactat ctggaaaatg gtaaatatga tttgaaatac agttacattc ttcaggatga
       61 ttcagaagaa gaacattata atgcagatac agtggtgaca ctgaaagatt ttccgaaaat
      121 ctccatgaca ttggcagaga tgtttgagaa tgtagaataa tatgagcagc atgaaagccc
      181 tcgggagtta atcctggggg gcttttggta tagccaaatc cgttgaagta tgttattata
      241 tgcatcagtt ctcagaatcg ggaatattca gctgagataa gaaaggacac aacaatcata
      301 tgaacatagg aaatatcatg aagatcacag gcgcatggaa cacctttaag agcaatcatc
      361 ccaagttccc ggcattctgt caggcagttt cacgcaaagg acttaaagaa ggaagcatta
      421 ttgaaattgc gatcaccact cctgagggag agaagataga gacaaacctg aaagtaaagg
      481 acagtgacct ggaattactg aaacagttgt ctgacctgaa aatgtgaaaa agtttacaaa
      541 aattttattt gaataacagg tataaatctg ctatagtatt ctatgttggt taagttgatt
      601 attattcgtt cccatatata agaatgatag gttctcaaaa gaaagaagag gaagaaaatt
      661 gcgtaaaaaa tcacatattt tattagcaag ataccttgcg gatcagatgc agaccacagc
      721 aagcctgcag tctcacagaa aggctttctg cctgggcagc attctgccgg atatcaaacc
      781 gtcctttatc accaggagac acgagttcta cggaacattt gaagatgtca agaaccggat
      841 gaaagaactg gcagatatca gaccggatga gagtaaccag agagtttact ggcggcggtt
      901 cggcgaagtg atccactata tggcggatta ttttaccttt cctcataaca aaacatatac
      961 aggaagcttc agccagcata atcattatga gaaagtactt aagaacagac tgaaagaatg
     1021 tatacagcag ggagaagcat atgcatatct ggaaccggcc atccgcttcg cagatttccc
     1081 caccctgatc gattatatcg aagctactca tgagaaatat ctcaacaagc tccgcagcgt
     1141 ggaagaggat atccgcttta tcctgaatat gtgttttcag gtagtgcagg gactgataca
     1201 gatctgcatt ggtaataaga actttgcagg agccattcag gcagcctgac agagatacgc
     1261 taagaataca ggaaggctgt gtggaaatca tatccgcaca gccttcctgc attctgaaac
     1321 atatcttcct cactgcttgt tgcttttcgc aacttctgca gggggactta cttgattcgg
     1381 ttaaaatgtg acaaaaaaat gaattttggt caaatctgga atttccctgc cctgagctgt
     1441 tgatttcaaa aaatgattat gctagaataa aacaggttaa tatttcggga taattcactt
     1501 atgaaataac ataataatgt gaaaagtaat ttagacagta tgcccttata aacagggggt
     1561 aaggacatgc tcacagtaag aagggagcaa tttagatata tgaaaaacaa gtatcttacc
     1621 agattcttat gtctgacatt gatgtcagga atggtattat ccggtcctgc gtccgtactt
     1681 gcagcagagg atactgctgc gtatacagca gacggttctg atttcagcga ttccggtgat
     1741 tttgattcag gatcagagga tccggcgccg gcagatccga ctcctgcaga gccaacacct
     1801 gcgccggtag atccgacacc tgcagacccg acacctgcac cggtagatcc gacaccaaca
     1861 cctgcagagc caacacctgc accggcagat ccgactccga caccaacaga tcctactccg
     1921 acacctgcga atcctacacc aacaccgtct tcacagatta cgaaggcagc ccaggatgtg
     1981 atcgacagaa tcaaagagtt gaacgcacag accatcacgc tggagaagaa agccactgtt
     2041 cagagcatcc gcactgcata taatgccctg tctgatacag agaaggcaca ggtttctaat
     2101 tataatctgc tggttcaggc agaagcgact attgctcagc tggaagcgca gaagaacaat
     2161 gcgaatacga ataatccatt tacgagtaat accagtgctg cacagaccgg aactccggta
     2221 tattatgcgt ccaatatcca cgcaggtaag gatttctatc tggacagcct gaagaataac
     2281 tacaatctga ccttctcaga tgatttcgct tctgttatgg atgagatcga gaaggaatat
     2341 aaagagaaga acaaagta
//
LOCUS       DBMDXG010000348         2356 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9586_length_2356_cov_1.759202, whole genome shotgun sequence.
ACCESSION   DBMDXG010000348 DBMDXG010000000
VERSION     DBMDXG010000348.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9586_length_2356_cov_1.759202"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="ACZPD3_08880"
     CDS             <1..618
                     /locus_tag="ACZPD3_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR1416135.1"
                     /translation="QAFGKYRIYPKKWNIHLLSVSSHKIHGPKGVGFLYINSKAKVQP
                     LILGGGQQNGMRSGTDNVPGIAGLGVAAKMMYQNFDEKVEHLYQLKERMAEGLSKIDD
                     VVINGMPVREGAPHILSVSFLGIRSEVLLHTLEDRNIYVSAGSACSSHKRKPSATLSA
                     MGMSNAQIENTVRISFSEENTFEEVDYCLEVLNEVLPMLKRYARR"
     gene            804..1985
                     /gene="thiI"
                     /locus_tag="ACZPD3_08885"
     CDS             804..1985
                     /gene="thiI"
                     /locus_tag="ACZPD3_08885"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR1416136.1"
                     /translation="MIFHAFLIKYAEIAIKGKNRYIFEDALVKQMNIALSKVEGEFEV
                     KKEQGRIYVFCPEQYDYDETVEALQHVFGIVGICPVVIYEDQGFEQMAKDVVSYMKNC
                     HPNYDGSFKVYTRRAKKSYPITSMEVSAELGGRILDEFPDASVDVHEPDLTLSVEIRD
                     KIYVYSQTIKGAGGMPIGTNGKAMLLLSGGIDSPVAGYMIAKRGVKIDAVYFHAPPYT
                     SERAKQKVVDLAKQVAKYSGPIRLHVVNFTDIQLYIYDQCPHDELTIIMRRYMMRIAE
                     HFAKESRCLGLITGESIGQVASQTLQSLAATNEVCTLPVYRPVIGFDKQEIVERSWEI
                     GTYETSIQPFEDCCTIFVAKHPVTKPNLNVIHRSETKLEEKIDELMKTALETVEIIEI
                     D"
     gene            complement(2101..2337)
                     /locus_tag="ACZPD3_08890"
     CDS             complement(2101..2337)
                     /locus_tag="ACZPD3_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR1416137.1"
                     /translation="MKTVQISLNSIDKVKSFVNEITKYDNDFDLVSGRYVIDAKSIMG
                     IFSLDLSKPIDLNIHADGAALDTVMAIVGKYVTE"
BASE COUNT          780 a          480 c          516 g          580 t
ORIGIN      
        1 caggcattcg gtaaatatcg tatttatccg aagaaatgga atatccatct tctgtctgtc
       61 agctcacata agatccatgg accaaaagga gttggattcc tgtacatcaa cagcaaggca
      121 aaagtgcagc ctctgattct cggcggcggc cagcagaatg gcatgcgttc cggaacagac
      181 aatgtaccgg gaatcgcagg acttggtgtt gcagcgaaga tgatgtacca gaactttgac
      241 gagaaagtag agcatcttta ccagctcaaa gaacgcatgg cagaaggact ttctaagatt
      301 gacgatgtag taatcaacgg aatgcctgtc agagaaggcg caccgcatat cttaagcgtc
      361 agctttctgg gaatcagaag cgaggtattg cttcatacct tagaggacag aaacatttac
      421 gtatcagcag gaagcgcatg ctccagccat aagagaaaac caagcgcaac actttcagca
      481 atgggaatga gcaatgcaca gatcgagaac actgtacgta tcagcttctc agaggagaat
      541 acattcgaag aagttgacta ctgcctagaa gttctgaatg aagtactgcc gatgctgaaa
      601 cgctatgcac gcagataaca aataatagtt ataagtgata tttggaatgg aacctgagat
      661 aagctcagga atatttgaaa aggttgttaa tagcatttat atgaattcta tttgaaaatt
      721 acagatatta aatccagaaa aatattaatt ctaatattat ataaaacaat ttattataag
      781 caaaataaga gaaggagaaa caaatgatat tccacgcatt tttaataaaa tacgcagaaa
      841 tcgccatcaa gggcaagaac agatacatct tcgaagatgc ccttgtaaag cagatgaaca
      901 tcgccctttc caaagtagaa ggtgagtttg aagtaaagaa agaacaggga agaatctatg
      961 tattctgccc ggagcagtat gactatgatg aaactgtaga agccttgcag catgtattcg
     1021 gtattgttgg aatctgccct gtagtgatct acgaagatca gggattcgag cagatggcaa
     1081 aggacgtagt ttcctacatg aagaactgcc atccgaacta cgacggaagt ttcaaagtat
     1141 atacaagaag agcaaagaaa tcttatccga tcacatccat ggaagttagt gcagaactcg
     1201 gcggtcgtat cctggatgag tttccggatg caagcgtaga tgtacatgaa ccggacctga
     1261 ctctttccgt agaaatccga gacaagatct atgtatattc ccagaccatc aaaggcgcag
     1321 gcggaatgcc aatcggcaca aacggaaaag ctatgcttct cctttctggc ggaatcgaca
     1381 gcccggtagc aggttacatg atcgcaaaac gtggtgttaa gattgacgct gtatatttcc
     1441 acgctccgcc atacacaagc gaaagagcaa aacagaaagt tgtagacttg gcaaaacagg
     1501 tagcaaaata cagtggaccg attcgcctcc atgtggtaaa cttcacagat atccagctct
     1561 atatttacga ccagtgtccg cacgatgagc tgactatcat tatgcgtcgt tacatgatgc
     1621 gtattgcaga gcatttcgca aaagaaagca gatgtctcgg tctgatcact ggagaaagca
     1681 tcggtcaggt tgccagccag accctgcaga gccttgcagc aacaaacgaa gtctgcacac
     1741 tccctgtata ccgtccggtt atcggattcg acaaacagga aatcgtagaa agatcctggg
     1801 aaatcggaac ctatgaaaca tccattcagc cattcgaaga ctgttgtact attttcgtgg
     1861 caaaacatcc ggttaccaaa ccaaacctga acgtgatcca ccgttctgaa acaaaactgg
     1921 aagaaaaaat tgacgaactc atgaagacag cgctggagac tgttgagatt attgagattg
     1981 attaatatcg attacaaaca ttcggaatct gctgacgcta cttccttatg tttgaacttg
     2041 aaactctggt atacaaaaaa agaggaactg gtaaacagtt cctcaatgac tgcgaaaaaa
     2101 ttattctgta acgtatttgc ctacgattgc cataacggta tcaagagcag cgccatcagc
     2161 gtggatgttt aaatcgattg gtttggataa atcaaggctg aaaataccca tgatagattt
     2221 tgcgtcgatt acatatcttc cggatactaa atcgaagtca ttatcgtact ttgtaatctc
     2281 attcacgaat gactttacct tgtcgataga atttaaagaa atctgtacag tcttcatgtt
     2341 accatccctc cttgat
//
LOCUS       DBMDXG010000349         2355 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9592_length_2355_cov_1.743567, whole genome shotgun sequence.
ACCESSION   DBMDXG010000349 DBMDXG010000000
VERSION     DBMDXG010000349.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9592_length_2355_cov_1.743567"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="ACZPD3_08895"
     CDS             complement(<1..598)
                     /locus_tag="ACZPD3_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR1416138.1"
                     /translation="MTLTQMNYIITISETGSLNKAAEALYISQPSLTNAVKELEKELG
                     IIIFNRSGRGVTLTNDGTEFLMYARQIYGQYESVVEKYSEGGSYKKKFGVSTQHYSFA
                     VKAFVDMVQKFDVSEYEFAIRETKTADVISDVSTMKSEVGVLYLSDFNRKALLKLLHS
                     ANLEFHHLIDCQAYVYLWKNHPLANEKSISYSQLAKYPC"
     gene            complement(787..1908)
                     /locus_tag="ACZPD3_08900"
     CDS             complement(787..1908)
                     /locus_tag="ACZPD3_08900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR1416139.1"
                     /translation="MKVVVAVDSFKGSMTSMEAGMAVKAGILAAKKDAEVIVKPLADG
                     GEGTTDALIEGLKGERVDVTVTGPYHEPVQAYYGYLRESNTAVMEMATAAGITLSDKK
                     EPMTATTYGVGELILHAIGRGIRNFIIGIGGSATNDGGVGMLRAFGYKFLDEKGEDVG
                     EGGQALARIASVEISDKKELLSQCNFRIACDVTNPLCGSQGATYIYGPQKGVTPDILP
                     VLDAGMKNYAEVTAKVIGKDNQNAAGAGAAGGLGFAFLNYLDGELTPGIQLILDAVHI
                     EDEMKDADVVVTGEGRLDHQTAMGKAPVGVAKLGRKYGAKTIAFAGSVTKEAVACNEA
                     GIDAFFPIVRGISTLEEAMDPDTAKANMAATAEQVFRLL"
     gene            complement(1937..>2355)
                     /locus_tag="ACZPD3_08905"
     CDS             complement(1937..>2355)
                     /locus_tag="ACZPD3_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024060.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HIR1416140.1"
                     /translation="WGFTCIRRSVYMKPTILLINFQDQQKLRKLKMALLPFKLRIKTI
                     EPQDYSQPIGYLAGVKEVSQVQIPSALIPQEQMEKEMLILAGITGNLFDQVLYTLRKA
                     GTPVDYKAVLTEHNQNWNCMQLYKELEKEHNMYHPS"
BASE COUNT          600 a          625 c          416 g          714 t
ORIGIN      
        1 agcaggggta tttagccagc tggctgtagc tgatggattt ctcatttgcc agtggatgat
       61 ttttccacaa atatacatat gcctgacagt caatcaaatg atgaaactcc agattcgcag
      121 agtggagaag ctttaacagt gcctttctgt tgaaatcact gagataaagt actcccacct
      181 cgcttttcat ggtgctgaca tcactaatca catctgcagt ctttgtctcc ctgatggcaa
      241 attcatattc cgacacatca aacttctgca ccatatccac gaatgctttc actgcaaaag
      301 aataatgctg ggtggaaacg ccgaatttct tcttatagga accaccctca ctgtatttct
      361 caaccacact ttcatactgt ccgtatatct gacgggcata catcagaaat tctgtcccgt
      421 cattggtcag tgtcacacca cggccgctgc ggttgaatat aatgattcca agctccttct
      481 caagctcctt cactgcattg gtcagagaag gctgggaaat atacaacgcc tccgcagctt
      541 tatttaatga accagtttca gaaattgtga tgatataatt catctgtgtt agtgtcatgt
      601 tgtctctccc ttacatatcc ttagatcttt taatcaactt ttttcaaagt attttctctg
      661 gctgtaataa ttttactata tcatctgaag tttctcaaca ttgcaatttc tgcaaacaaa
      721 aatctcctgt gcagacatat caaatcgatt tcctatctgc ttcagaagat tttttatgaa
      781 aaataattac aacagtctga atacctgctc tgcagttgca gccatatttg cttttgcagt
      841 gtccggatcc attgcttctt ccagtgtgga aattccgcgc acgattggaa agaaggcatc
      901 tattcctgct tcattgcagg caactgcctc cttcgtaaca cttcctgcaa atgcaatggt
      961 ttttgcgcca tatttcctgc caagcttcgc aacgcccacc ggggcttttc ccatggcagt
     1021 ctgatgatcc agacggcctt cccctgtcac aaccacatct gcatccttca tctcatcctc
     1081 tatatggaca gcatccagaa taagctgtat tcccggtgtc agctctccat ccagataatt
     1141 cagaaatgca aatccaagtc cgcctgccgc acctgcacct gctgcattct ggttatcttt
     1201 accaatcact ttggcagtaa cctctgcata gtttttcatg cctgcatcca gaaccggaag
     1261 gatatcgggg gtaactccct tctgcggtcc atagatatat gtagctccct gagaaccgca
     1321 gagaggattg gtcacatcac aggcaatcct gaaattacac tgggaaagaa gttccttctt
     1381 gtctgaaatt tctacagatg caatccttgc aagtgcctgt ccaccttccc ctacatcttc
     1441 tcccttttca tccagaaatt tatatccaaa ggctctcagc attcccactc caccgtcatt
     1501 agtggcgctt ccgccaatac cgataatgaa gttgcggata cctctgccga ttgcatgaag
     1561 gatcagttct cccactccat aggtggtagc ggtcataggc tccttcttat ctgaaagtgt
     1621 gatacctgct gctgttgcca tttccataac tgcggtatta ctttccctga gatatccata
     1681 atatgcctga accggctcat gataaggacc tgtaactgtc acatccacac gttctccctt
     1741 cagaccttcg atcagcgcat ccgtagttcc ctctccaccg tcagccagag gttttacgat
     1801 cacttctgcg tccttttttg ctgcaagaat acctgctttg acagccattc ctgcttccat
     1861 ggaagtcata cttcccttaa atgaatcaac tgcaactaca accttcatag ccaaaacctc
     1921 caactatttc tattatttaa gacgggtgat acatattatg ttctttctcc agttctttat
     1981 aaagctgcat acagttccag ttctgattat gctccgtcag aactgccttg taatccacag
     2041 gtgtacctgc tttccgcaag gtatagagca cctggtcaaa cagattcccc gtgattcctg
     2101 caagaatcag catttccttc tccatctgct cctgcgggat cagcgctgat ggaatctgca
     2161 cctgagaaac ctctttcact cctgccagat atccgattgg ctgactatag tcctgaggtt
     2221 ctatggtttt aatccgaagc ttaaaaggca gcaacgccat ctttaacttg cgaagcttct
     2281 gctgatcctg aaaatttatt aataaaatag ttggtttcat ataaacactc cttctgatac
     2341 aagtaaatcc ccacc
//
LOCUS       DBMDXG010000350         2355 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9594_length_2355_cov_1.454747, whole genome shotgun sequence.
ACCESSION   DBMDXG010000350 DBMDXG010000000
VERSION     DBMDXG010000350.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9594_length_2355_cov_1.454747"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /gene="hflK"
                     /locus_tag="ACZPD3_08910"
     CDS             <1..700
                     /gene="hflK"
                     /locus_tag="ACZPD3_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR1416141.1"
                     /translation="DVDFFVEYRILEPVKYLYNSEEPEDILKNISQSCIRTVIASYDV
                     DEVLTTGKGEIQSKIKEMILKQMEEQDLGIQLVNITIQDSEPPTQEVMKAFKAVETAK
                     QGKETALNNANKYRNEKLPEAEAEADQIIQDAEAQKQVRINEAEAEVARFNAMYEEYV
                     KNPEITKKRMFYEAMEDVLPGMKIVIDNGDGVQKVLPLDSFTGDSSENSTETLNTDPQ
                     SAQDTQNINDSENE"
     gene            786..1652
                     /gene="hflC"
                     /locus_tag="ACZPD3_08915"
     CDS             786..1652
                     /gene="hflC"
                     /locus_tag="ACZPD3_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0098797 - plasma membrane protein complex
                     [Evidence IEA];
                     GO_process: GO:0052547 - regulation of peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR1416142.1"
                     /translation="MKGKKIGILIGVSAVVVAVGASVTVTQQNEYKLIRQFGKVDRVI
                     SSSGISFKIPFIESTQSLPKETLLYDLAASDVITKDKKTMISDSYVLWKISDPLKFAQ
                     TLNSSVESGESRINTAVYNATKNAISSMSQDQVITSRDGELSDMVMEAIGTNMDQYGI
                     ELLKFETKQLDLPDDNKEAVYERMISERDNIAATYKAEGNSEAKVIRNKTDKEVAIQI
                     SDAKKQAEILGAEGEQEYMKILAQAYGEEDRSEFYSFVRSLDALKTSMKGEDKTVILS
                     ADSPIAQIFEGK"
     gene            1810..2163
                     /locus_tag="ACZPD3_08920"
     CDS             1810..2163
                     /locus_tag="ACZPD3_08920"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR1416143.1"
                     /translation="MLTCIRSKRQRDAAEYALRTIQSSCIRPYIKTIYLYGSCARGEE
                     KYSSDVDLFLELSESFRSRPELKKYLYLLKSEVSSEELDDAETDLKIVVGDDWKRNKM
                     LYYTNVRKEGIQIWP"
     gene            2154..>2355
                     /locus_tag="ACZPD3_08925"
     CDS             2154..>2355
                     /locus_tag="ACZPD3_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR1416144.1"
                     /translation="MALNSYFDFAENDFRYFKASYDAGIVANMMGAMAQGICEKYMKH
                     LISEYYKPDDAIQQKDFENILRT"
BASE COUNT          850 a          347 c          572 g          586 t
ORIGIN      
        1 tgatgtggat ttctttgttg agtacagaat tttagaaccg gtaaaatatc tttataattc
       61 agaggaaccg gaagatattc taaaaaatat ttctcaaagc tgtatccgta cagtaatcgc
      121 aagttatgat gtggatgaag tgcttaccac cggaaaaggt gagatccaga gtaagattaa
      181 agaaatgatc ctgaagcaga tggaggaaca ggatctgggg attcagcttg tgaatatcac
      241 aatccaggat tcagaaccgc ccacacagga ggttatgaaa gcatttaaag cagtagaaac
      301 tgccaagcag ggaaaagaaa ctgctttgaa taatgccaat aaatacagaa acgaaaagct
      361 tccggaggct gaggcagaag cagaccagat cattcaggat gctgaggcgc agaagcaggt
      421 aaggattaat gaggcggaag ctgaggttgc acgttttaat gccatgtatg aggagtatgt
      481 taagaatccg gagatcacaa agaagcgtat gttctatgag gcaatggaag atgtgctgcc
      541 gggaatgaag attgtgattg ataatggaga tggagtgcag aaggttctgc cgttagattc
      601 ttttacaggg gatagttcag agaattcaac agaaacactg aacacagacc cgcagagtgc
      661 tcaggataca caaaatataa atgattctga gaacgaataa agaaatgaca gaaaatattt
      721 catataaaag tgttttggta aatgaaatac gaaggataga gatataattt ggaaaggact
      781 aatttatgaa aggtaagaaa attggaatat taattggtgt gtctgcagtg gttgtagctg
      841 tcggagcgtc ggtaacagta actcagcaaa atgaatataa gctgatccgt cagtttggta
      901 aggttgacag ggtgatcagc tcatcgggaa tcagttttaa gattccattt atagagagta
      961 cacagtcatt gccaaaggaa acactgctgt atgatctggc agcatctgat gtaattacaa
     1021 aagataagaa aacgatgatc agcgacagct atgttctctg gaaaatcagc gacccgctta
     1081 aatttgcaca gacattaaac tcttctgtgg aaagtggtga aagccgaatt aatactgccg
     1141 tgtataatgc cactaaaaat gcgatcagca gtatgtcaca ggatcaggtg atcacaagtc
     1201 gtgacggaga actgtctgat atggttatgg aggcaatagg aacaaacatg gatcagtatg
     1261 gaatcgaact tctcaaattt gaaaccaagc agctggattt gccggatgat aataaggaag
     1321 ctgtatatga acgaatgata tctgaacgag ataatattgc agcgacttac aaggcagagg
     1381 gaaactctga agcaaaggtg attcgaaata aaaccgacaa agaagtagca attcagattt
     1441 ctgatgctaa aaaacaggca gagatcctgg gggcggaagg ggagcaggaa tatatgaaaa
     1501 ttcttgcgca ggcttatgga gaagaggaca gaagtgagtt ttattcattt gtgagaagcc
     1561 tggatgcgtt gaagacatct atgaaaggtg aggacaaaac agtgatactg tctgcagatt
     1621 caccaatcgc tcagatcttt gaaggaaaat aaaagaaagt aacaataata cactctaatt
     1681 tttcaccaaa taaatggaag atagacatag aagataagga atgttataat ttttttacaa
     1741 agaaatcctg aagcaaaaaa tgtaagtaca atctggaaat aatatacaga gaaaacggag
     1801 ggatagaaaa tgctgacatg tatcagatca aaacggcaga gagatgcagc ggagtatgct
     1861 ttacgaacta ttcagtcatc atgcataagg ccttatatta agacaatata tctgtatggc
     1921 agctgtgcac ggggagaaga gaaatatagc agtgatgttg atctgtttct ggaactgtca
     1981 gagtcattcc gctcccggcc ggaacttaaa aaatatcttt atcttctaaa atcagaagta
     2041 tcctctgagg aattggatga tgcagaaacg gatctgaaaa ttgtagttgg cgatgactgg
     2101 aaaagaaaca aaatgctgta ttatacaaat gtgcgaaagg agggaattca gatatggcct
     2161 taaatagtta ttttgatttt gcagaaaacg attttcgata ttttaaggct tcttatgatg
     2221 cggggatagt ggcaaatatg atgggtgcca tggcacaggg aatatgcgaa aaatatatga
     2281 agcatctgat ctcagaatac tataaaccgg atgatgctat acaacagaaa gattttgaaa
     2341 atattttgcg tacac
//
LOCUS       DBMDXG010000351         2345 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9632_length_2345_cov_1.428253, whole genome shotgun sequence.
ACCESSION   DBMDXG010000351 DBMDXG010000000
VERSION     DBMDXG010000351.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9632_length_2345_cov_1.428253"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="ACZPD3_08930"
     CDS             complement(<1..405)
                     /locus_tag="ACZPD3_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1416145.1"
                     /translation="MNKVKKMLYSQKVAPYVFVIPFVISLLVFWLYPLISGIVMSFQD
                     ISFGGSQWVGLKHYQKLASDKFFKTAVFNSVEYMLLTLLLLIPIPMMLAVLMESRLTK
                     AKGVWKVIMYIPALTSVVISGMYMITFHTPLAL"
     gene            complement(672..2066)
                     /locus_tag="ACZPD3_08935"
     CDS             complement(672..2066)
                     /locus_tag="ACZPD3_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1416146.1"
                     /translation="MKRRKLFALLMTAAMAVSSMSMAVNVFAEEDTTEEAAESEEPAE
                     GEPTAVTTVGPDDGTKFEMWSFVDLHNEFYGQMVDKWNEENPDKQIQITFSTYPYSDM
                     HNKLMMSLQAGSGAPDLCDIEIGQFPNYSGDDSPLYSMNDALAPYEDTMVQSRLDVYS
                     KADGTRLGVPFHVGATVMYWNADLLESYGLDYKSVKTWDDYTKLGEELKEASNGEVYL
                     TSVDTGGVDWMWLAMAENGEDWTGGPDGTVNVQLDSVKEMLTMQQNWLNDGIAMVSTD
                     GHVDLEAGFSNIMEGKIASFPKAMWYMSRFKDYMPEMEGKYDITTCPVFEEGQKCSVG
                     IGGTGTVVTNQCENPELAAEWLAWAKCSEEGENLIWNELGFDVCNTALWSDEDFAYDE
                     SNTYNTFFRVKPYEVLNELAENDAIGTIYTTKNSPTLNDYMCTTTLNNVLEDGMDVDE
                     ALQDAQDYLDFECE"
BASE COUNT          644 a          531 c          406 g          764 t
ORIGIN      
        1 caaagccaaa ggtgtatgga aagtgatcat gtacattccg gaaataacta cagatgtaag
       61 tgccggaatg tacatgatca ctttccatac acctttggct ttggtaagac ggctttccat
      121 cagtactgca agcatcatcg ggatcggaat cagcaggagc agtgtcagaa gcatgtattc
      181 cacactgtta aatacagctg tcttgaagaa cttatcggaa gccagtttct gataatgctt
      241 cagaccaacc cactgagaac cgccaaatga aatatcctga aaactcatta caataccact
      301 gatgagcgga tataaccaga ataccagaag gctgataaca aacggaatta cgaatacgta
      361 gggtgccacc ttctgagaat agagcatctt tttcacttta ttcacaatga attcctcctc
      421 ttctaaaaat agcagtaact tttcagcatc atatggaatc atggtttact gaacagttac
      481 agataatttc tttttttctt attaccatta tcctgaaaag aaatcagact ccacatcctg
      541 tgaaaatctc atttctcacc aggataacag taaaggaggt cagggaatgc gtttccctgg
      601 cctcccatat catttcggtc actgcagtaa ccgttcatta ttcacttgat cagtgaacta
      661 ttatcatctt attattcaca ttcgaaatca agataatcct gtgcatcctg aagcgcttca
      721 tctacatcca tgccatcctc taatacattg ttcagagttg ttgtacacat gtaatcgttc
      781 agtgtcggag agttctttgt tgtgtaaata gttccgattg catcgttttc agcaagttca
      841 ttcagaactt cgtatggttt tacacggaag aatgtgttat aagtattgga ttcatcatat
      901 gcgaaatctt cgtcagacca taatgctgtg ttgcagacgt caaatccaag ttcgttccag
      961 atcaggtttt caccttcttc agagcattta gcccatgcaa gccattcagc tgcaagttca
     1021 ggattctcac actgatttgt tacaacagta cctgtaccac cgataccaac ggagcatttc
     1081 tgtccttctt caaatacagg gcatgttgtg atgtcatatt tgccttccat ctccggcatg
     1141 taatctttga aacggctcat gtaccacata gctttcggga aggaagcaat tttaccttcc
     1201 atgatgttgg agaaaccagc ttcaaggtca acatgtccgt ctgtggatac cattgcgatt
     1261 ccatcgttta accagttctg ctgcattgtg agcatttctt ttacagaatc aagctgtacg
     1321 tttacagttc cgtccggacc gcctgtccag tcttcaccat tctcagccat tgcaagccac
     1381 atccagtcaa caccgcctgt atcaacagaa gttaagtaca cttcaccgtt ggaagcttct
     1441 tttaattctt caccaagttt tgtataatca tcccatgtct ttacagattt gtaatctaat
     1501 ccataagatt caagaagatc tgcgttccag tacataacag ttgcacctac atggaaagga
     1561 acaccaagtc ttgttccgtc agctttggaa taaacatcaa gtcttgactg caccattgta
     1621 tcttcataag gagcaagtgc atcattcatg gaatacagag ggctgtcatc accggaatag
     1681 ttcgggaact gtccgatctc gatatcgcag aggtcaggag caccggatcc tgcctgtaag
     1741 gacatcatta atttgttatg catatcagag tatggatatg tactgaatgt aatctggatc
     1801 tgtttgtccg gattctcttc attccactta tctaccatct gaccataaaa ctcattatgc
     1861 aggtctacga atgaccacat ctcgaatttt gttccatcat caggacctac ggttgttact
     1921 gctgtaggct ctccttcagc cggttcttcg ctctcagcag cttcctctgt tgtgtcttct
     1981 tctgcaaaga cattcactgc catgctcata ctggacactg ccatagccgc agtcataagt
     2041 aacgcgaaca attttcttct cttcataaaa agatcctcct tttttgcatt tctttgcgct
     2101 cttccggttt caatgatgcc attgtacaaa ccccataatc atttcgtatt tttttaaaat
     2161 atatagtcca tctttttgtt atggtcattt tgcacaactt ttttattttc agctccggtt
     2221 tatgttgata ttatataatt ctttcctgta aatgtcaata tatttttgat ttatttttat
     2281 tattttagtt ttatctaaaa taaatgcccg ttatttattc atcaatctat ctattttttt
     2341 gctat
//
LOCUS       DBMDXG010000352         2344 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9637_length_2344_cov_1.604547, whole genome shotgun sequence.
ACCESSION   DBMDXG010000352 DBMDXG010000000
VERSION     DBMDXG010000352.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9637_length_2344_cov_1.604547"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="ACZPD3_08940"
     CDS             complement(<1..429)
                     /locus_tag="ACZPD3_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR1416147.1"
                     /translation="MEVLLAGNTGYVTETFIEESFPECDVVVLGNEMLKSNRKKNILS
                     RPFIKDEKELKEIFETYEFERIVYFSNYLTMHGRMTGELEQLRQILQLCKKNKEIRML
                     YLTGQESIYNITSGKTLLVSAAENVVMEYGNMYQINTKILR"
     gene            731..>2344
                     /locus_tag="ACZPD3_08945"
     CDS             731..>2344
                     /locus_tag="ACZPD3_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR1416148.1"
                     /translation="MEKEKTNPVLGYRTAGSAAETATGDMLYREMKAIGLTDVTRDTF
                     SLDGWEFEKAVLKFTDDDGQEHTFQMGGYQTNFETDGFEDYELVYLGKGTAADYEGIN
                     VKGKLVMVEINQRDEWWISFPVYQAYLRGAAALIAVQANGYGEIAESALNAQDIAGPD
                     FAPAFSLSQADARILKRSLRNKISACEDNTTDSEDTHNTPEQDAALLRRSSLKVSLDA
                     RSRVIPDTTAGNITGKIQGTETDSMILLSAHYDSYFDGFQDDNAAVAMMLGIARSLVK
                     GGYKPAHTLVFCAMAAEEWGIIDSKYDWSTGAYNQVFRVHPDWQGKVIADLNFELPAH
                     AHNRQDAIRCTYEYADFIRQFTDSLTVPKEAYPDGITVLSPIETWSDDFSMAIAGIPS
                     TVNDFSAGPFMQNYYHSQYDNQDVYQEAVYQFHHECYLKLIMAIDRLVLPPMNFSNTM
                     NAVTESIHENALSFADPEQNVLLRNLQTITASAENIYDKICQINAEYATLSDSDTRIA
                     FRHKWEYAGLELLKTFRKAQDYLVRLNWQD"
BASE COUNT          673 a          557 c          460 g          654 t
ORIGIN      
        1 tctcagaatc ttggtgttta tctgatacat atttccatat tccataacta cattttctgc
       61 tgcggatacc agaagtgttt ttccacttgt aatattataa atgctctcct gcccggtcag
      121 atacaacatt cttatttcct tattcttttt gcataactga agtatctgtc tcagttgttc
      181 cagttctccg gtcatccttc catgcatggt cagataattt gaaaaataaa caattctttc
      241 aaactcatat gtttcgaaaa tctctttcag ttctttctca tcttttatga acggacgaga
      301 taatatattc ttttttcgat ttgatttcag catctcattt cccagtacaa ctacatcaca
      361 ttcaggaaat gattcttcta taaaagtttc tgttacatat cctgtatttc ctgccaacaa
      421 tacttccata tttattcacc tcaataaagt gaagtataac aaagttattt tttcttttca
      481 atatcagcat ctttacagaa aatagctttt ttctcaacaa ctgttgtctt gttcctgctg
      541 ctgttctatg ctaaaatatt tccagtataa aatcataaac cacagacaga tcagtaccct
      601 gacatttccg gcactgatca tatctgatca tattatacaa actgtcagga aagaggcgat
      661 tacattgaac attgaagatt cctgtatttc ctgcattgac acagactggt cctaccagct
      721 tgccagacgc atggaaaaag agaaaacaaa ccctgttctg ggctatcgta ctgcaggttc
      781 cgctgctgaa acagctacag gagatatgtt atacagggaa atgaaagcta tcggacttac
      841 cgatgtaacc agagatacat tctcactgga cggatgggaa ttcgagaagg cagtcctgaa
      901 atttacagat gatgacgggc aggaacacac ctttcagatg ggcggatatc agacaaattt
      961 cgagacagac ggatttgaag attacgaact cgtttatctg ggaaaaggaa ctgctgcaga
     1021 ttatgaaggc ataaacgtaa aaggaaagct ggttatggtt gagatcaacc agcgagacga
     1081 atggtggatc agcttccctg tttatcaggc ttatctgcgc ggcgctgctg ccctgatcgc
     1141 agttcaggca aacggctacg gagaaatcgc agagtctgcg ctgaatgcac aggatatcgc
     1201 aggtcctgat ttcgcacctg cattctctct gtctcaggca gatgcacgta tcctgaagcg
     1261 ttctctccga aataaaattt ccgcctgcga agacaacacc actgattcag aagataccca
     1321 taacactccg gaacaggatg ctgctttatt acgcagatcc tcattaaaag tatcactgga
     1381 tgcccgttcc agagtgattc cggacacaac agcaggcaat atcacaggta aaatacaggg
     1441 tactgagaca gactctatga tcctgctgtc tgcacactat gattcctatt tcgatggatt
     1501 ccaggacgat aatgcagcgg tcgccatgat gctcggcatt gcccgttcac ttgtaaaggg
     1561 cggctataag cctgcccaca ctctcgtttt ctgcgcaatg gctgccgagg aatggggaat
     1621 cattgattcc aaatacgact ggtctacagg agcctacaat caggtattcc gcgtacatcc
     1681 tgactggcag ggtaaagtga tcgcagacct gaactttgaa cttcctgcac acgcacacaa
     1741 tagacaggat gccatccgct gtacctatga atatgcagat ttcatcagac agtttacaga
     1801 cagcctgact gtcccgaagg aagcatatcc tgacgggatc acggttctct cccccatcga
     1861 gacatggtct gatgatttct ccatggcaat cgcaggtatc ccatccacag tcaatgattt
     1921 cagcgccggg cccttcatgc agaattatta tcattcccaa tatgataatc aggatgttta
     1981 ccaggaagct gtctatcagt ttcatcatga atgctatctg aaactgatca tggccattga
     2041 cagacttgtc ctgccaccga tgaatttcag caataccatg aacgcagtca cagaaagtat
     2101 ccacgaaaat gcactctcat ttgctgatcc tgaacagaat gtcctgctca gaaacctgca
     2161 gacaattacc gcttctgccg aaaatattta tgacaaaatc tgccagatta atgcggaata
     2221 tgccacactt tcagactccg acacccgcat tgctttccgc cacaaatggg aatatgccgg
     2281 actggagctg ctgaaaactt tccgcaaggc acaagactat ctggtacgtc tcaactggca
     2341 agat
//
LOCUS       DBMDXG010000353         2341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9655_length_2341_cov_1.130804, whole genome shotgun sequence.
ACCESSION   DBMDXG010000353 DBMDXG010000000
VERSION     DBMDXG010000353.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9655_length_2341_cov_1.130804"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="ilvC"
                     /locus_tag="ACZPD3_08950"
     CDS             <1..832
                     /gene="ilvC"
                     /locus_tag="ACZPD3_08950"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR1416149.1"
                     /translation="KVYTAAEAAKQADIIMILINDELQAKLYKESIEPNLEEGNMLMF
                     AHGFNIHFGCIKPPKNVDVTMIAPKAPGHTVRSEYQAGKGTPCLIAVEQDYTGKAQDL
                     ALAYGLGIGGARAGLLETTFRVETETDLFGEQAVLCGGVCALMQAGFETLVEAGYDPR
                     NAYFECIHEMKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKIITEDTKKAMKKILS
                     DIQDGTFAKDFLLDMSDAGAQVHFKAMRKKASEHQSEKVGEEIRKLYSWNGEDKLINN
                     "
     gene            complement(1068..>1261)
                     /locus_tag="ACZPD3_08955"
     CDS             complement(1068..>1261)
                     /locus_tag="ACZPD3_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416150.1"
                     /translation="ANSSKNKTYKWISSLSERFSLVEMIATGIVYLKDNRTDTYQPFI
                     SGNGKYCRYDVEKGQIVEL"
     assembly_gap    1262..1271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1272..1579)
                     /locus_tag="ACZPD3_08960"
     CDS             complement(<1272..1579)
                     /locus_tag="ACZPD3_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416151.1"
                     /translation="MNKELYDEAIRSNILSRKLIEQLMESMNYSNISFINWTVEVLKI
                     IRTRLERGDKITDEVSGITYDIKSFRNFVSTNFSSYITSQVFDAPDKAEKVYFSLEAT
                     "
     gene            1943..2128
                     /locus_tag="ACZPD3_08965"
     CDS             1943..2128
                     /locus_tag="ACZPD3_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416152.1"
                     /translation="MEIEEEFISGFCRTCNGGQTVCCEYTMEGDKRTLTFMDCAHDRC
                     VNYAACEIYKQAHEMER"
BASE COUNT          733 a          403 c          501 g          694 t
ORIGIN      
        1 taaggtatat acagcagctg aagcagctaa acaggcagat atcatcatga ttcttatcaa
       61 tgatgaatta caggctaaac tttacaaaga atccatcgag ccaaaccttg aagaaggcaa
      121 catgttaatg tttgctcatg gtttcaatat ccatttcgga tgcatcaaac caccaaagaa
      181 tgttgatgtt acaatgatcg ctcctaaagc acctggtcac acagtaagaa gtgagtatca
      241 ggctggaaaa ggaacacctt gtcttatcgc tgtagagcag gattacacag gaaaagctca
      301 ggatcttgca ctggcatatg gtcttggaat cggcggagca agagctggtc ttcttgaaac
      361 tacattccgt gtagagacag agacagacct ctttggtgag caggctgtac tttgcggtgg
      421 tgtttgcgca cttatgcagg ctggtttcga aacacttgtt gaagctggtt atgacccaag
      481 aaatgcatac tttgaatgta ttcatgaaat gaagctgatc gtagacctga tctatcagtc
      541 aggattcgca ggaatgagat attctatctc caacactgct gaatatggtg attacatcac
      601 aggacctaag atcatcacag aagatacaaa gaaagctatg aagaaaattc tttctgatat
      661 ccaggatggt acatttgcaa aagacttctt acttgatatg tctgatgcag gtgcacaggt
      721 tcacttcaaa gctatgagaa agaaagcttc tgagcatcag tctgagaaag ttggcgaaga
      781 aatccgtaaa ctttacagct ggaatggtga agacaaactt atcaacaact aatctgaata
      841 tgattaaatt aaaatcccct gctgaatggc aggggatttt ttatgtcggg caggatcgca
      901 ctgcgacgga gaggaattat gtcgctgaag cgacccgccg caggcggaga atcctgcaag
      961 caggattctt tcttgtatgt actgactcaa tgattgttgt ttgttctgta ttatgcaatt
     1021 cttaagatta tgcataatat aacaagatgc aaatcaatct cagcatttta taattcgaca
     1081 atctgtcctt tttctacgtc atatctgcag tacttaccat ttccggaaat aaatggctga
     1141 tatgtatctg tcctgttatc tttaagatat acaattccgg tagcaatcat ctccactaat
     1201 gaaaagcgtt cgctgagact ggaaatccac ttatatgttt tgttctttga tgaatttgcc
     1261 annnnnnnnn ntcggtggct tccagagaaa aatatacttt ttctgcctta tcaggagcat
     1321 caaagacctg acttgtaata taggaagaaa aattggtgct tacaaaattt cggaaagatt
     1381 ttatatcata agtgattccg gatacttcgt ctgtgatttt atctccgcgt tccagacgag
     1441 ttctaataat ctttagtacc tctacagtcc agttgataaa agagatattg ctgtaattca
     1501 tactttccat caactgttcg attagtttgc gtgacaggat attggagcgt attgcctcat
     1561 cataaagttc tttgttcatg acattttccc tcttttttga ttatttgtaa ctattcagca
     1621 gtctactatc ccgcgaggtg ttttggcatg aacatgccaa aatcccgaga catacaagtc
     1681 aaaatcccat cgccgaaggc gatttttaat ggattttgac tcgtaactgt gaaggtactg
     1741 aacagttacg attattttat gtttgttata gtgtttccgc acatttctat gttatactga
     1801 ttataaatct gtttgttgta aaatgcaaca aaaaacggac gaataaataa aaatatttgt
     1861 gaacagtaac gaatatattg ttgcctgtat tttggtttct cggaacgata atgagacata
     1921 aggaaaattg aggagggata aaatggagat agaagaagag tttatttcgg gattttgcag
     1981 aacatgtaat ggagggcaga ctgtctgctg cgaatatacg atggaaggag ataaaagaac
     2041 cctcacattt atggattgtg cacatgacag atgtgtgaat tatgctgcct gtgaaatcta
     2101 caaacaggca catgaaatgg aacgataaga atatgtgagg gtgtacgaaa agcagtgaac
     2161 tgcaaaatgt attgctgaga acggagggga tagtaatccg caaatgctgt aattatcgga
     2221 ttaaccggag ttggaatcaa acatatatga aaaataggca tctttgaatg cagaatagtt
     2281 atttctggaa atagggacag aggaaatgcc ggtagtgacc atagttcttg tgaactgttt
     2341 t
//
LOCUS       DBMDXG010000354         2340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9657_length_2340_cov_1.697186, whole genome shotgun sequence.
ACCESSION   DBMDXG010000354 DBMDXG010000000
VERSION     DBMDXG010000354.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_2340_cov_1.697186"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            139..405
                     /gene="tenpIN"
                     /locus_tag="ACZPD3_08970"
     CDS             139..405
                     /gene="tenpIN"
                     /locus_tag="ACZPD3_08970"
                     /inference="COORDINATES: protein motif:HMM:NF047358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system TenpIN family
                     toxin"
                     /protein_id="HIR1416153.1"
                     /translation="MEIDYAVYSLSDEFYEKYPNPPYKELLKKKERRYACLLIQSHYG
                     YFICIPYRTEISHKYAYHFRKSSRSQKHRSGLDYTKIAIIKDIS"
     gene            539..1249
                     /gene="tsaA"
                     /locus_tag="ACZPD3_08975"
     CDS             539..1249
                     /gene="tsaA"
                     /locus_tag="ACZPD3_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR1416154.1"
                     /translation="MVPIAHIENNFPTKFGIPRQSGRVGALKARIVFEPEYRNVDACR
                     GLEEFSHIWLIWEFSEAKRTKWSPTVRPPRLGGNVRKGVFATRSPFRPNSIGLSCVKL
                     EKVALDEPDSPVLYVEGADLMNGTPIFDIKPYIPYADCHPEATGSFTEYSKDHHLNVE
                     FPQELLERIPGESREALIAVLADDPRPAYQNDPERSYGMPFGEKDIHFRVDGDILRVY
                     NVTEFVKKQQESSADCGK"
     gene            1395..>2340
                     /locus_tag="ACZPD3_08980"
     CDS             1395..>2340
                     /locus_tag="ACZPD3_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR1416155.1"
                     /translation="MKKIVKFGGSSLANAEQFQKVGEIIRSDESRRYVVPSAPGKRFD
                     GDTKVTDLLYKCYNTAVEGEDFIPILQEIKGRYYEIIRGLNLDLSLEDEFAQIEADFK
                     AQAGTDYAASRGEFLNGKVMAAYLGYEFVDAADVIRFDKNGNLDAEKTDRLLSKKLAK
                     CEHAVIPGFYGAGEDGKVKTFSRGGSDVTGSLVAKAIKADLYENWTDVSGFLVTDPRI
                     VKNPEVIESITYRELRELSYMGATVLHEDAIFPVRKEGIPINIKNTNRPEDKGTFIVE
                     STCKKPKFTITGIAGKKGFCSINIEKSMMNSEVGFGRKV"
BASE COUNT          742 a          403 c          559 g          636 t
ORIGIN      
        1 cggtaaaata aatataaccg agagatgaga tgagtgacgg ttctgactca tcggtaaaat
       61 aaaatatgac cgagcgaaga gtataacgag agggagttca ttttccctct cgtttatatg
      121 tatataagga gaagaaaatt ggaaattgac tatgctgttt atagcctttc agatgaattt
      181 tacgagaaat atccgaatcc gccatataaa gaacttttaa agaaaaagga aagaagatat
      241 gcttgtttac ttattcaaag tcattatgga tattttatat gcattccata tagaacagag
      301 atttcccata aatatgcata tcactttcgc aaatcgtctc gttcgcaaaa acatagatca
      361 ggacttgatt atactaaaat tgctattata aaagatattt cgtaactatt cagcagtcta
      421 ctatcccgcg aggtgttttg gcatgaacat gccaaaatcc cgcaagcagg attctttctt
      481 gtggattttg actcgtaact gtgaaggtac tgaacagtta caaaaggaga ataatacgat
      541 ggttccaatc gcacatatag aaaataattt tccaaccaaa ttcggtattc ccaggcagag
      601 tggccgtgta ggggcgctta aagcgagaat cgtattcgaa ccggaatatc gcaatgtgga
      661 tgcctgtcgt ggtctggagg agtttagtca tatctggctg atctgggagt tttcggaggc
      721 aaagaggaca aagtggtcac cgactgtccg cccgcccaga cttgggggaa atgtgagaaa
      781 aggagttttt gccacaagat ctccctttcg tcccaattcc ataggattat cctgtgtgaa
      841 gcttgagaaa gtagctctgg atgagccgga cagccctgta ctttatgtag aaggtgcaga
      901 tctgatgaat ggaacgccga ttttcgatat taaaccatat attccttatg cagactgcca
      961 tccggaagca accggaagtt ttaccgaata ttccaaagat catcatctga atgtagaatt
     1021 tccccaggag cttctggaaa gaatacccgg ggaaagcaga gaggctctga tcgcagttct
     1081 tgcagatgat ccccgtcctg cttatcagaa tgatccggaa cggtcttacg gaatgccttt
     1141 tggagagaag gatattcatt ttcgggtgga tggagatatc cttcgtgttt ataatgtgac
     1201 agaatttgta aaaaaacagc aggaatcttc ggcagattgt ggaaaataac cattttcgga
     1261 tatttctgta agaaaggctt gcaaaattgg cgtttattca atacaatatt aaacagactt
     1321 tatgttggta gagtgacaaa gactgatggg agatcggtct gaaaatattt agtgacatat
     1381 gaggagcagg aaaaatgaag aaaattgtta aattcggagg aagttctctg gcaaatgcag
     1441 aacagtttca gaaagtaggg gagatcatca gaagtgatga gagcagacgt tatgtggttc
     1501 cgtccgctcc cggaaagaga tttgacggag atacaaaggt tacagattta ctttataaat
     1561 gctataatac tgcagttgaa ggggaggatt ttataccgat cctgcaggaa atcaagggaa
     1621 gatattatga gatcatccgt ggactgaacc tggatctttc tctggaagat gaatttgctc
     1681 agatcgaagc tgatttcaag gcacaggcag gcacagacta tgcagcatcc agaggagaat
     1741 ttctcaatgg taaggtgatg gcagcatatc tgggttatga atttgttgat gcagcagatg
     1801 tgatccgttt tgataagaac ggaaatctgg atgccgagaa gacagacaga cttctgtcca
     1861 aaaagctggc aaaatgtgaa catgccgtta tccctggatt ttatggcgct ggtgaagatg
     1921 gaaaagttaa aactttctcc agaggaggtt ctgatgtaac cggttctctt gtggcaaagg
     1981 ctattaaggc agacctctat gaaaactgga cagatgtttc cggtttcctg gtaacagacc
     2041 cacgtattgt taaaaatccg gaagtgattg agagcattac ctacagagaa ctcagagagc
     2101 tttcttatat gggtgcaact gttcttcatg aagatgcgat cttcccggtt cgtaaagaag
     2161 gaattccgat caatatcaaa aacacaaaca gaccggaaga caagggtact tttattgttg
     2221 agagtacatg caagaaaccg aaatttacaa tcacaggtat tgcaggtaag aagggattct
     2281 gttctatcaa tattgagaaa tccatgatga acagtgaggt tggtttcgga cgtaaggtac
//
LOCUS       DBMDXG010000355         2335 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9686_length_2335_cov_2.002238, whole genome shotgun sequence.
ACCESSION   DBMDXG010000355 DBMDXG010000000
VERSION     DBMDXG010000355.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9686_length_2335_cov_2.002238"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            11..328
                     /locus_tag="ACZPD3_08985"
     CDS             11..328
                     /locus_tag="ACZPD3_08985"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR1416156.1"
                     /translation="MAVRVSKTDFEEKVLKEKLPVLVDFYSDSCLACKKLAPVLGNAE
                     DDYEDKIKVYKVNTNFDVELAEQYEVQANPTLILFKDGQAKDRKTGALKQAELNSWIE
                     GLL"
     gene            357..563
                     /gene="thiS"
                     /locus_tag="ACZPD3_08990"
     CDS             357..563
                     /gene="thiS"
                     /locus_tag="ACZPD3_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR1416157.1"
                     /translation="MFITVAGEKKEVEDGLTLPELIAQENVEMPEYVTVSINEEFVAS
                     EDKESTVLKEGDNVEFLYFMGGGC"
     gene            564..1400
                     /locus_tag="ACZPD3_08995"
     CDS             564..1400
                     /locus_tag="ACZPD3_08995"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR1416158.1"
                     /translation="MAMTDEQIERYSRHIILKEVGVKGQKKLLKGKVLIIGAGGLGAP
                     AAMYLAAAGVGTIGIVDADEVDLSNLQRQIIHSTADIGKAKVKSAKETMNAMNPDVEV
                     KTYRMFVDASNIRELIREYDFIIDGTDNFPAKFLINDACVLEKKPFSHAGIIRFKGQL
                     MTYVPGEGPCYRCVFKNPPPKDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYLIGVGDL
                     LTGYLLTFDALTMEFHKVKLPKDTHDCAVCGDHPTILEPIDYEQEVCEETAGRFATRD
                     EQ"
     gene            1437..1868
                     /locus_tag="ACZPD3_09000"
     CDS             1437..1868
                     /locus_tag="ACZPD3_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR1416159.1"
                     /translation="MAIIRMRDHLYDEIVNYAKEHLPEEACGLLAGVETGEGREIRKV
                     YFLENKDHAEDHFTLDPRDQINAIRDMRANGLKPLGNWHSHPSSPSRPSVEDIRLAFD
                     SKASYLILSLMADYPVLNSFHIEGGEWTKEDLRIYSEEYYF"
BASE COUNT          765 a          405 c          594 g          571 t
ORIGIN      
        1 tggaagactg atggcagtac gggtaagcaa gacggatttt gaagagaaag tcctgaagga
       61 aaaacttccg gttctggtgg atttttattc agattcctgc ttggcgtgta aaaagctggc
      121 accggttctc ggaaatgcgg aagacgatta tgaggataag attaaagtat ataaagtaaa
      181 tacgaacttt gatgtggagc tggcagagca gtatgaagtg caggcaaatc cgacgttgat
      241 cctttttaaa gacggacagg cgaaagaccg gaaaacagga gcattgaaac aggcagagtt
      301 aaacagctgg attgaaggat tactctaaaa agagagcagg cataaaggag aaaaatatgt
      361 ttatcacagt agcaggagag aaaaaagaag tagaagacgg actgacactt ccggaactga
      421 tcgcacagga aaatgtagag atgccggaat atgtaaccgt ttctattaat gaagaatttg
      481 ttgcatcaga ggataaagag tctaccgtat taaaagaggg agacaatgta gaatttcttt
      541 atttcatggg aggtggatgc taaatggcaa tgacagatga acagatcgag cgttattccc
      601 gtcacatcat cttaaaagaa gtgggtgtaa agggacagaa gaagctcctg aaaggaaaag
      661 tactgatcat cggtgcaggc ggtcttggtg cacctgcggc aatgtatctg gcagcagcag
      721 gtgtgggaac aatcggtatt gtggatgcgg acgaggtaga tctttccaat ctgcagagac
      781 agatcatcca cagcacagca gatatcggaa aagcaaaggt aaaatcagca aaggaaacca
      841 tgaatgccat gaatccggat gtggaagtaa aaacttaccg gatgttcgta gacgcatcca
      901 acatccgcga actgattcga gaatatgatt ttattattga cggaactgat aatttcccgg
      961 caaaatttct gatcaatgat gcatgtgtac tggagaaaaa accattttcc catgcaggta
     1021 tcattcgttt taaaggtcag ctgatgacct atgtgcccgg ggaaggtcca tgttaccgct
     1081 gcgtattcaa gaatccgccg cccaaggatg cagttcctac ctgtaagcag gcaggtgtca
     1141 tcggagcaat gggaggtgtc atcggttccc tacaggcaat ggaagcaatc aagtatctga
     1201 tcggtgtagg cgatcttctg acaggatatc ttctgacttt tgatgcgctg acaatggaat
     1261 tccataaggt taaactgccg aaggatactc atgactgtgc agtatgcgga gatcatccga
     1321 caatcctgga accgatcgac tacgagcagg aggtctgtga ggaaacagca ggccgatttg
     1381 ccacaagaga tgagcagtaa atacaagaaa aacctgatgc aaaacaggag aaaagtatgg
     1441 ctataataag aatgagagat catctctatg atgagatcgt taattatgca aaagagcatc
     1501 ttccggagga agcctgcggg cttttggccg gagtggaaac cggggaggga agggaaatcc
     1561 gaaaggtata ttttctggag aataaagatc atgcagaaga tcacttcacc ctggatccca
     1621 gagaccagat aaatgcgatc agggatatgc gtgccaatgg attgaaaccg ctgggaaact
     1681 ggcattccca tccgtcttct ccatcccgtc cgtcagtgga ggatatccgt cttgcttttg
     1741 attcaaaggc aagttatctg atcctgtcac tgatggcgga ctatccggta ttgaacagct
     1801 ttcatattga aggtggggaa tggacaaaag aggatctaag gatttattca gaagaatatt
     1861 atttttaaag atttacagac aggataacag aggcagaaat gtttgaagca ggattatttg
     1921 tggaaacatt tctgcctctg tttatactgt cagcatataa aaattccgta cattgtaaaa
     1981 gtatagtaat cctcgtaaat aatgcatctt aatccagccc atcatagcat gaaatttcca
     2041 taataaagtc gtggcaaaat catgtcaact gcagaataag gcttgaaaga ttatgcaaaa
     2101 taatgtaaac tcaaagagtt aagatatgta gacattatgt atgcctgtaa cgtttaagta
     2161 tagtatattc ccacaatggc tggacggttg cagataacag atcggttact ataataattt
     2221 gagcatctcc ggtgcggata tccggagatg ctttgctttg gaaattgtgt gtgaggggta
     2281 taaaaaaagt atgagaaaag attctgacaa tgcaaaactg ttggaaccgg gggaa
//
LOCUS       DBMDXG010000356         2314 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9777_length_2314_cov_1.527338, whole genome shotgun sequence.
ACCESSION   DBMDXG010000356 DBMDXG010000000
VERSION     DBMDXG010000356.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9777_length_2314_cov_1.527338"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="ACZPD3_09005"
     CDS             <1..557
                     /locus_tag="ACZPD3_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR1416160.1"
                     /translation="AFRNEEVSRIILTRPAIEAGEKLGFLPGDLQSKVDPYLRPLYDA
                     LYQIMGADSFAKNMERGLIEVAPLAYMRGRTLDNAFIILDEAQNTTPAQMKMFLTRIG
                     FGSKVIITGDASQKDLPRDTTSGLDVAMKVLKKIDDIGFCQLTSKDVVRHPLVQQIVQ
                     AYDAYEKKQKPERPARRRNGVNER"
     gene            554..1048
                     /gene="ybeY"
                     /locus_tag="ACZPD3_09010"
     CDS             554..1048
                     /gene="ybeY"
                     /locus_tag="ACZPD3_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR1416161.1"
                     /translation="MNMQIDYETERELGIDYETLAVKVADKVLEMEKCPYDAQVNLVL
                     TDNEEIERVNTEFRDIARPTDVLSFPMIPFETPAGYDIVEEDESYFDLDTDELLLGDI
                     MISVDKVFAQAEEYGHSVTREFCFLVAHSMLHLLGYDHMTPGEAAVMETKQAKALEEL
                     GITR"
     gene            1153..>1848
                     /gene="proB"
                     /locus_tag="ACZPD3_09015"
     CDS             1153..>1848
                     /gene="proB"
                     /locus_tag="ACZPD3_09015"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR1416162.1"
                     /translation="MNYRERLKDKKRIVIKIGSSSLTHSETGRLNLRKLEVLARELSD
                     LRNQGKDVILVSSGAIATGVAALGMHEKPTELKGKQACAAVGQARLMMIYQKLFSEYN
                     QLSAQILMTKNTMVNNVNRKNAQNTFDELLSLGVIPIVNENDSISTYELQNLEKFGDN
                     DTLSAMVAALVRADLLILLSDIDGLFTDDPNTNPDAKFIDVVENLDDNLLNMGKGTSG
                     SKVGTGGMATKLTA"
     assembly_gap    1849..1858
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1859..2025
                     /locus_tag="ACZPD3_09020"
     CDS             <1859..2025
                     /locus_tag="ACZPD3_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR1416163.1"
                     /translation="AAQIASAAGVDMVIANGADFHIIHKITEGRRYGTLFVSQSKEEV
                     YLIDIIDRLL"
     gene            2119..>2314
                     /locus_tag="ACZPD3_09025"
     CDS             2119..>2314
                     /locus_tag="ACZPD3_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIR1416164.1"
                     /translation="MEVEKIDRINALAHKAKSVGLTEEEKKERELLRKEYIASVRMNL
                     RAQLDNVDVQQEDGTIINLGE"
BASE COUNT          725 a          447 c          565 g          567 t
ORIGIN      
        1 cagccttccg aaatgaggag gtcagcagga tcatcctgac acgtcctgct attgaggccg
       61 gtgagaagct gggatttctt ccgggagatc tgcagagcaa ggtagatcct tatctgagac
      121 cactctatga tgcactgtac cagatcatgg gagcggacag ctttgcgaaa aatatggagc
      181 gcggcctgat cgaggttgca ccgcttgcat atatgcgcgg acgtacactg gataatgcat
      241 ttatcattct cgatgaagca cagaatacta ctccggcaca gatgaagatg ttcctgacac
      301 gtatcggatt tggttccaag gttatcatca ccggagatgc ctcacagaaa gatcttccaa
      361 gagataccac atccggactt gacgtggcaa tgaaagtact gaaaaaaata gatgacattg
      421 gcttctgcca gcttaccagc aaggatgttg tccgtcatcc tctggtacag cagatcgtac
      481 aggcttatga tgcctatgag aagaaacaaa aacctgaaag accggcaaga cgcagaaacg
      541 gggtaaatga acgatgaaca tgcagattga ttatgagaca gagcgtgaac tggggatcga
      601 ctatgagaca ctggcagtaa aggtagctga caaggttctt gaaatggaaa aatgtcccta
      661 tgatgcacag gtgaatcttg tacttacaga taacgaagag attgagagag ttaatactga
      721 gtttcgcgat atcgcaagac ctacggatgt tttatccttc cctatgattc cttttgagac
      781 accggcagga tatgatattg tggaggaaga tgaatcttac tttgatctgg atacagacga
      841 acttcttctt ggagatatca tgatctcagt tgacaaggta tttgcgcagg cagaggaata
      901 cggacacagt gtgacaagag aattctgttt tctggttgca cacagtatgc tgcatctgct
      961 gggatatgac catatgactc ccggggaagc ggcagtcatg gagacaaaac aggcaaaagc
     1021 cctggaggag cttgggatta cacgctgatc ttcaaattct gttattgcag gcaatttctg
     1081 atggattttg tctcgtaact gtaaagacac tgaacagtta ctaaaaatat atttatatgg
     1141 agtaacaaaa agatgaatta ccgggaacga cttaaagata aaaaacgtat tgtaattaag
     1201 atcggttctt cttccctgac tcattcggag acaggaagac tgaaccttcg caaactggaa
     1261 gtgctggcaa gagagctcag tgaccttcgc aatcagggaa aagatgtcat actggtttct
     1321 tcaggagcta tagcaacagg tgtggcagct cttggaatgc atgagaaacc cacagaactg
     1381 aaaggaaaac aggcatgtgc agcagtagga caggccagac tgatgatgat ttatcagaaa
     1441 cttttttctg aatataatca gctttccgct cagatcctta tgaccaaaaa tactatggtt
     1501 aataatgtta accgaaagaa tgctcagaat actttcgatg aactgctcag tctgggagtg
     1561 attcccattg taaacgagaa tgactccatt tccacctatg agcttcagaa tctggagaaa
     1621 ttcggagata atgatacatt gtctgcaatg gttgccgcac ttgtacgggc agatctgctg
     1681 atcctgttat cagatattga tggtcttttt acagatgatc ccaatactaa tcctgatgca
     1741 aaattcatcg atgtggtaga aaatctggat gataatctgc ttaacatggg aaaaggtact
     1801 tctggaagta aggtaggaac cggcggaatg gctactaagc tgacagcann nnnnnnnnca
     1861 gcagcacaga tcgcttctgc agcaggcgta gatatggtga tcgcaaacgg agccgatttc
     1921 catattatcc ataagatcac agagggacgc aggtacggta cgctgtttgt aagccagtcc
     1981 aaagaagaag tttatctgat cgatattatt gacagactgt tataataccg gaaatttacc
     2041 ggtgccaaat gacattcaca gagtaaatgg ctgtgtttac agacagactg ccgtagacaa
     2101 aaaacaggag gaagaactat ggaagttgag aaaatcgacc gcattaatgc acttgcacat
     2161 aaagccaaaa gtgtggggct tactgaagaa gagaaaaaag aaagagaact gctgcgcaag
     2221 gaatatattg catctgtccg catgaacctt cgcgctcagc tggacaatgt ggatgttcag
     2281 caggaagacg gaacaatcat taatctgggt gaaa
//
LOCUS       DBMDXG010000357         2309 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9797_length_2309_cov_2.284873, whole genome shotgun sequence.
ACCESSION   DBMDXG010000357 DBMDXG010000000
VERSION     DBMDXG010000357.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9797_length_2309_cov_2.284873"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="ACZPD3_09030"
     CDS             complement(<1..229)
                     /locus_tag="ACZPD3_09030"
                     /inference="COORDINATES: protein motif:HMM:NF039334.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6050 family protein"
                     /protein_id="HIR1416165.1"
                     /translation="MTIEDVIKKSILPLAFMLFWFWMVSTIMKVSGQTDLFWWIFLSG
                     LPFGIHKMRLILIPKGMDITATLGMAALSVII"
     gene            complement(219..386)
                     /locus_tag="ACZPD3_09035"
     CDS             complement(219..386)
                     /locus_tag="ACZPD3_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416166.1"
                     /translation="MALKIVRRYDANGRELTEKDMETFRLDSPVVRKILANLNREVAE
                     GIREREKKGDD"
     gene            complement(522..1427)
                     /locus_tag="ACZPD3_09040"
     CDS             complement(522..1427)
                     /locus_tag="ACZPD3_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR1416167.1"
                     /translation="MPSKKRATPVSLQPLDALFGTNEETTNGISEIAIGSLHPFTNHP
                     FQVRDDKKMEELAESITQYGVLVPGIVRLRESGGYELVAGHRRKRACELSGLEKMPVI
                     IKDLTDDEATVIMVDSNIQREELLISEKAFAYKMKYEALKRQGKRSDLTSCQVGKKLA
                     AEEVSQNTGDSSRQILRYIHLTELVAELLELADEKKLPFNTAVELSYLRTEEQQILFQ
                     YMSNHNMVPSMKQAKELKQISKEQMLTYSEIDQICMNESTEKVQVQIPAKKLKQYFPE
                     TYTKDQMEEIIFMLLASWAEREGKE"
     gene            complement(1417..2202)
                     /locus_tag="ACZPD3_09045"
     CDS             complement(1417..2202)
                     /locus_tag="ACZPD3_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR1416168.1"
                     /translation="MAKVISIVNQKGGTGKSACTANLAVGLAQKNMKVLIVDADPQSD
                     VSAGFGYRDCDESNETLTALMDTVMKDEDIPSDCYIRHQAEGIDIICSNIGLAGTEVQ
                     LVNAMSREYVLKQILYGIKDQYDAIIIDCMPSLGMITINALAASDEVLIPVEASYLPI
                     KGLQQLLKTIGKVRKQINPKLQVGGILFTMVDAHTNDARNNMELLRNVYGSQIHIFDN
                     YIPFSVRMKEAVREGQSIFSYDPKGKATEAYRRVTEEVLKDAI"
BASE COUNT          608 a          558 c          341 g          802 t
ORIGIN      
        1 caatgatgac acttaatgca gccatcccaa gtgttgctgt tatatccatt ccttttggaa
       61 tcaggatcag ccgcatttta tgtattccaa atggaagtcc ggaaagaaat atccaccaga
      121 acaagtctgt ctgtccactg actttcatga tggtggatac catccagaac cagaatagca
      181 taaatgccag tggcaatatt gattttttaa ttacgtcttc aatcgtcacc ttttttctcc
      241 ctctctctga ttccttccgc cacctcccga ttaagatttg ccaatatttt tctaaccaca
      301 ggagaatcca atcgaaaggt ttccatatct ttctcagtaa gctcccttcc gttcgcatca
      361 tatcttcgta caatctttaa agccatactt tcacttcctt cctgttgata atcctaatgt
      421 tttcaacaaa tgtttaaact tctctctttt catccgttta acacgtatct tttctatctt
      481 cttcagataa acttgtattc tacttgccgt atcttcattg ctcattcttt cccctctctt
      541 tctgcccacg atgccagaag cataaatata atttcttcca tctgatcctt tgtatatgtc
      601 tctggaaaat attgtttcaa tttcttcgcc ggaatctgca cctgaacttt ttcagtactt
      661 tcattcatgc aaatctggtc aatctctgaa tacgtcaaca tctgctcttt tgaaatctgc
      721 ttcaattcct ttgcctgctt catggacggt accatattgt gattactcat atactgaaat
      781 aatatctgct gctcttcagt tctcaaataa gaaagttcaa cagctgtgtt aaagggaagc
      841 ttcttctcgt ctgccaattc taatagctct gctactaact ctgtcagatg aatatatcga
      901 agaatctgtc tggaactatc tcctgtattc tgactgactt cttctgccgc caacttcttg
      961 ccaacttggc aagaagttaa atcagatcgt tttccctgac gctttaacgc ctcatatttc
     1021 attttataag caaatgcctt ttcgctgatc agcagctctt ctcgttgaat gttggaatct
     1081 accataatca ccgtcgcttc atcatctgta agatctttga tgataaccgg cattttctct
     1141 agtccagaaa gttcacatgc tcgttttcgc cgatgaccag ctaccagctc ataaccaccg
     1201 gactctctca accgcacaat accaggaacc aacacgccat attgtgtaat actttccgct
     1261 aattcttcca ttttcttatc atctcttact tgaaacgggt gattcgtaaa tggatgaaga
     1321 cttcctattg ctatctcact aattccattg gttgtctctt cgtttgttcc aaacaatgca
     1381 tcaagcggct gtaaggaaac gggagttgct ctttttttag atggcatctt tcagcacctc
     1441 ctccgtcact ctccgataag cttctgtagc tttgcctttc ggatcataag agaaaatact
     1501 ctgtccttct ctgactgctt ccttcattct tacagaaaat ggaatataat tatcgaagat
     1561 atgaatttga cttccatata catttctgag aagttccata ttattcctgg catcattcgt
     1621 atgagcatcg accatcgtaa acagaattcc accgacttgc agcttcggat tgatttgctt
     1681 acggacctta ccgattgttt ttaataactg ctgcaatcct ttgattggca ggtaagacgc
     1741 ttcaaccgga attagtactt catctgatgc agccagtgca ttgattgtga tcattcccaa
     1801 tgatggcata caatcaatga taattgcatc atattgatcc ttgataccat ataaaatctg
     1861 tttcaataca tattccctgc tcattgcatt taccaactgt acttccgtac ctgccagtcc
     1921 aatgttagaa cagataatgt ctattccttc tgcctgatgc ctgatataac agtctgaagg
     1981 aatatcttca tctttcatta ctgtatccat aagagctgta agtgtttcat tactttcatc
     2041 acaatcacga tatccaaatc ctgcggatac atcagactgt ggatcggcat caacgattag
     2101 cactttcata ttcttttgtg ctaatcctac tgctaaatta gctgtacatg ctgattttcc
     2161 cgttccccct ttttggttta caatagaaat tacttttgcc ataatctttc ccttctttct
     2221 tgttaaataa aaagactgaa tcagacagct gagatataaa tctcatgcga gctaattcag
     2281 tccaaaacat atttttattc aattatcac
//
LOCUS       DBMDXG010000358         2300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9851_length_2300_cov_1.365166, whole genome shotgun sequence.
ACCESSION   DBMDXG010000358 DBMDXG010000000
VERSION     DBMDXG010000358.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9851_length_2300_cov_1.365166"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(343..1500)
                     /locus_tag="ACZPD3_09050"
     CDS             complement(343..1500)
                     /locus_tag="ACZPD3_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1416169.1"
                     /translation="MSEKIADVIKETENNKADRQEQYFFRTDQLTVGYDGKPLIREIN
                     IQLKKGEILTLIGPNGAGKSTILKSITRQLATISGTVYLDKEKMAKMTNKEVSQKLAV
                     VLTERMRPELMTCEDIVATGRYPYTGTLGILSAEDKTKVKKSMETVHAWDLKDRDFTA
                     ISDGQRQRILLARAICQEPEIIVLDEPTSFLDIRHKLELLTILKQMVLDHQLTVIMSL
                     HELDLAQKISDKVICVHGEYIEKYGAPEEIFTSEYIRKLYGITRGSYNAAFGCVEMDP
                     PRGEPQVFVIGGNGSGIPYYRKLQRQGIPFAAGVLHTNDVDCQVAGALADQVITEKPF
                     ESISQESYDKAVELMKKCQKVICPLKDFGTVNAANRELLRLARELGILAEL"
     gene            complement(1579..>2300)
                     /locus_tag="ACZPD3_09055"
     CDS             complement(1579..>2300)
                     /locus_tag="ACZPD3_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR1416170.1"
                     /translation="SGAKLVVALLMIYFLSRSISMGSAAMIIAAFIGSMISMGFVLLV
                     AKKVHNMSMLVISGVMIGYICSAVTDFVVTFADDSNIVNLHNWSLGSFSGMSWGNVKV
                     MAIVVLLTMVIVFFMSKPIGAYQLGEVYAQNMGVNIKLFRIGLILLSSILSACVTAFA
                     GPISFVGIAVPHIVKSLLKTARPLLVIPACFLGGAVFCLFCDLIARTVFAPTELSISS
                     VTAVFGAPVVIYIMIRRQKRN"
BASE COUNT          634 a          543 c          454 g          669 t
ORIGIN      
        1 ctatgtgcac cgaaggtatc ataaaaggca atgaaaaagt cattaatgac ctgttcaatg
       61 ttatcctgct cattgtatgc cgggataaca atgtataact tatccatatt tctctccttt
      121 ggatgattga tggtatccat tatagcatac atatacatga aataataata ttatttcgaa
      181 aacgatgtac tatagctgtt actgttcact gctaatatcc cgcgaggtgt tttttgtgag
      241 cgaaggtcac agaaaacccg agacatactg cgcgaattta tgcgattagc ataaattctg
      301 tgcatcgcga agcgtgttgc tgggaacgga gtgaacagta acctatagct ctgccagtat
      361 tcccagctcc cgcgcaaggc gaagcagttc cctgtttgcg gcattcactg ttccgaagtc
      421 tttcagagga cagataactt tctgacattt tttcatcaat tccacagcct tatcataaga
      481 ttcctgtgaa atactttcga aaggtttctc tgtaatcacc tggtctgcaa gtgcaccggc
      541 tacctgacag tctacatcat tcgtgtgaag cactcctgcc gcaaacggaa ttccctgtct
      601 ctgcagtttt cgataatacg gaattccgct tccattaccg ccaattacaa acacctgagg
      661 ttcacctctg ggcggatcca tctccacaca tccaaaagca gcattatagc ttcctctggt
      721 aattccatac aacttccgaa tatactcaga agtaaagatt tcttccggag caccatattt
      781 ttctatatat tctccatgta cacagataac tttatcagat attttctgtg caagatccaa
      841 ctcatgcagc gacatgatca ctgtaagctg atgatccagc accatctgtt tcaaaatggt
      901 aagaagttcc agcttatgac ggatatccag aaaagaagtc ggctcatcca gtacaatgat
      961 ctccggctcc tgacagatag cccgggcaag aaggattcgt tgtctctgac catcactgat
     1021 ggctgtaaaa tccctgtctt ttaaatccca tgcatggacg gtttccattg atttctttac
     1081 ctttgtctta tcctctgcgg agagtattcc caaagttcct gtataaggat aacgccctgt
     1141 ggctacaatg tcttcgcagg tcatcagttc cggacgcata cgctctgtga gcacgactgc
     1201 cagtttctga gagacctctt tattggtcat ctttgccatt ttctctttat ccagataaac
     1261 agtaccgctg atcgtggcaa gctgtctggt gatgctttta agaatagtgg attttccggc
     1321 accgttagga ccgatgagag tgaggatttc tccctttttc agctgaatat tgatttctct
     1381 gataagaggt tttccgtcat atcccacagt cagctgatcc gtacggaaaa aatactgttc
     1441 ctgccggtct gctttgttat tttctgtttc ctttattaca tctgctattt tctctgacat
     1501 attttttcct gttttccttt ttatgctgca tcttatagta atctgcccgg atagcgtatt
     1561 ctgctcctgc cttttatatc aatttctctt ctgtctgcgg atcatgatat agattacaac
     1621 cggtgcgcca aacactgctg ttacggaact gatactgagt tctgtaggtg caaatacggt
     1681 tctggcgatc agatcacaga aaagacagaa tactgcccct cccagaaagc aagccgggat
     1741 cacaaggagc ggtctggctg ttttcagcag gctttttaca atgtgaggca ctgcaatgcc
     1801 aacaaaagaa attggtcctg caaaagctgt cacacaggca gacagaatac tggataagag
     1861 aatcagtcct atgcggaaca gcttgatatt cactcccata ttctgggcat aaacttcacc
     1921 cagctgatag gcaccgatag gcttagacat aaagaacacg atcaccattg tgagcagaac
     1981 tactattgcc attactttca cattacccca ggacattcct gaaaaacttc ccagtgacca
     2041 gttgtgaaga ttgacgatat tggagtcatc cgcaaaggta actacgaaat ctgtaactgc
     2101 agaacagatg taaccgatca tgacaccgct gataaccaac atggacatgt tgtggacttt
     2161 ttttgcaacc agcaggacga atcccatgga aatcatagaa ccgataaacg ctgcaatgat
     2221 catggctgca gaacccatgg aaatagatct gctcagaaaa taaatcatca gaagtgcaac
     2281 taccagcttg gctcctgaag
//
LOCUS       DBMDXG010000359         2298 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9862_length_2298_cov_1.203914, whole genome shotgun sequence.
ACCESSION   DBMDXG010000359 DBMDXG010000000
VERSION     DBMDXG010000359.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9862_length_2298_cov_1.203914"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="ACZPD3_09060"
     CDS             <1..312
                     /locus_tag="ACZPD3_09060"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HIR1416171.1"
                     /translation="SYITIEDLNVKGMMKNRHLSKAVASQKFYEFRTKLQAKCREKGI
                     ELRVVDRWYSSSKTCHCCGAVKKDLKLSDRIFKCSCGYVEDRDFNAALNLRDVTTYEI
                     A"
     gene            508..1752
                     /locus_tag="ACZPD3_09065"
     CDS             508..1752
                     /locus_tag="ACZPD3_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012308.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HIR1416172.1"
                     /translation="MIRKQLLIGILAAMTVALPAVGIYAGSAEASEIQSSNGDTVSGN
                     KGASIDKAVTLQNSVKMTDQISAAVNGENIMFSPTSLNFALGMIAEGAKGETKEVLCN
                     YLGTDDFASYAKEYLNKIKEYNTEDESYGYKSKLKIADAVWVDNNLTLQEEFKNSVTN
                     GFGAEVENVDFSAAEKTCGIINSWCDKNTEGLIPKIITPDLINDTTGLCLTNSLYFES
                     GWSGEPWNVSDTEEKFGNNEKTKYMTCAGDRYYENDKATAFDREYANGLSFVGILPVD
                     EGDFTLEDLDIGGLLKSQPEYDEVQCKMPKLDFETTAILNDILSSLGLDNIFFSNADF
                     SGIADKNVNVDTILQKTKLELDENGTKAAAVTAVIMECMSAVEEKEPVIKNVELTRPF
                     AFLIYDSSNDEILFMGKVMTVS"
     gene            complement(1792..>2298)
                     /locus_tag="ACZPD3_09070"
     CDS             complement(1792..>2298)
                     /locus_tag="ACZPD3_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OLD family endonuclease"
                     /protein_id="HIR1416173.1"
                     /translation="LMIRDGDGKDAEELASSLCRYYEARNREDMDRLPRVTRENVLIL
                     KYYSFENYFLDPKIMEKIGVIKSEDDFYEILLKKWNEYLYKLKSGQHLTEMIGHALKD
                     TTDIREHMEEIRICLRGHNLYDIFYGRFRKNETEILKSYIEEAPRDTFKDILDAIDRF
                     VYFENRKK"
BASE COUNT          750 a          422 c          496 g          630 t
ORIGIN      
        1 tcttatataa cgattgaaga cttaaatgta aaaggcatga tgaaaaacag acatctttca
       61 aaagcagttg catcacagaa gttttatgaa tttagaacaa aacttcaagc caaatgcagg
      121 gaaaaaggaa ttgaacttcg agtagtagat agatggtatt catcgtctaa aacctgtcac
      181 tgttgcggtg ctgtcaagaa agatttaaaa ctttcagata ggatctttaa atgcagttgt
      241 ggttatgtcg aagataggga tttcaatgct gctttaaatc tgagagatgt cacgacttac
      301 gaaattgcat aacgaaacgc aaacgtgagt atgtaccgaa ggctatttcg ggaatttacg
      361 actatggagt gtacaagaac ctgtgagtag atatgatctc gttcaatcca aagcatacac
      421 gatgaagtag taagtagaac tcgtgagagt ctacattatc tcgatgtgcg tatatttgca
      481 cacgctttga gtggcaggaa atgccttatg ataagaaaac aactcttaat cggaatactg
      541 gcagctatga ctgtcgcact gccagctgtc ggcatatatg ccggttccgc agaagccagt
      601 gaaatccaaa gcagcaatgg agacacggtg tccggtaata aaggagccag tatcgacaaa
      661 gccgtgacat tacagaattc cgtaaaaatg acagaccaaa tctctgcggc agtcaatgga
      721 gaaaatatta tgttcagtcc cacaagcctt aattttgcgc tgggcatgat cgcagaaggt
      781 gcaaaaggag agacaaaaga agttctatgt aattacctgg gaaccgatga ttttgcatct
      841 tatgcaaagg aatatctgaa caagattaaa gaatataata ctgaagatga gagttacgga
      901 tataagtcaa aattaaagat tgcagatgca gtctgggtgg ataataatct gacattacag
      961 gaagaattta aaaattcagt aacgaacggt tttggggctg aagtggaaaa tgtagatttt
     1021 tccgctgcag agaaaacatg cggtatcatt aattcctggt gtgacaaaaa tacggaaggg
     1081 ctgatcccga aaatcataac cccggatctg atcaatgata ctaccggact gtgcttaact
     1141 aattcactat attttgagtc cggatggagt ggagaaccct ggaatgtttc tgatacagaa
     1201 gagaagtttg gaaacaatga aaagacaaaa tacatgacct gtgccggaga caggtattat
     1261 gaaaatgata aggcaacagc atttgacagg gagtatgcaa acggactgag ttttgtcgga
     1321 atcctgcctg tcgatgaagg cgattttaca cttgaggatc tggatattgg tggacttctg
     1381 aaatctcagc cagagtatga cgaagtacaa tgtaagatgc caaaacttga ttttgagact
     1441 accgccatcc taaatgacat actctcaagc ctggggcttg acaatatatt tttcagcaac
     1501 gctgatttct ccggtattgc agacaaaaat gtaaatgtag atacgatact gcagaaaaca
     1561 aaactggaac tggatgaaaa cggcacgaaa gcagcagcag tgacagcagt tatcatggaa
     1621 tgtatgtcag ctgtagagga gaaagaacca gttatcaaaa atgtggaact cactcgtcct
     1681 tttgccttcc tgatctatga cagtagcaat gacgaaatac tgtttatggg aaaagtaatg
     1741 acagtttcct gatgaaaagg ggatgccatg cgtggcatcc ctcttcatga attatttttt
     1801 tctgttctca aaataaacaa aacggtcaat tgcatccaga atatctttaa aagtatccct
     1861 tggagcctcc tctatatagg atttgaggat ttcagtttcg tttttacgga atcttccgta
     1921 aaagatatca tataaattat gtccgcggag acagatacgg atttcttcca tatgttctct
     1981 gatatctgta gtatctttga gagcatggcc gatcatttct gtgagatgct gaccgctttt
     2041 taatttgtat agatattcat tccacttttt caacaggatt tcgtagaaat catcctcact
     2101 ttttatcact ccgatttttt ccattatctt cggatccagg aaataattct caaaggaata
     2161 atacttcagg atcagaacat tttctcgagt cacacgcggc agtctgtcca tatcctcacg
     2221 gttacgagcc tcataatacc tgcacaggga agaagccagt tcttctgcat ctttaccatc
     2281 cccatcccgg atcatcag
//
LOCUS       DBMDXG010000360         2292 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9891_length_2292_cov_1.623916, whole genome shotgun sequence.
ACCESSION   DBMDXG010000360 DBMDXG010000000
VERSION     DBMDXG010000360.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9891_length_2292_cov_1.623916"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="ACZPD3_09075"
     CDS             complement(<1..148)
                     /locus_tag="ACZPD3_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005428586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR1416174.1"
                     /translation="MKLLKDRILKDGVVKPGNILKVDSFLNHQMDITLINEIGKEFKR
                     RFSDC"
     gene            501..728
                     /locus_tag="ACZPD3_09080"
     CDS             501..728
                     /locus_tag="ACZPD3_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416175.1"
                     /translation="MKEYELIWEIFNKCPRNQMRDVFVEELELEDPEEYIRNKFKGKE
                     VSYEKTVLEDGTIIFDITTSQIKQRCSFTEI"
     gene            848..1195
                     /locus_tag="ACZPD3_09085"
     CDS             848..1195
                     /locus_tag="ACZPD3_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR1416176.1"
                     /translation="MAEEQKTHTHVLEDGTVVEHSHGEHGHHHSHAHTKAVLNRMSRA
                     IGHMESIKRMIEDGRDCAEVLIQLSAVKSAINNTGKIILQDHIEHCIVDAVEHGDKEA
                     IKELEVAIDRFVK"
     gene            1496..>2292
                     /locus_tag="ACZPD3_09090"
     CDS             1496..>2292
                     /locus_tag="ACZPD3_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR1416177.1"
                     /translation="MAKECNNTSCDKASCEGCSSSKKQQSFQAEMNAQSNVKHVIGVV
                     SGKGGVGKSFVTGSLANMMAAQGYKVGILDADITGPSIPKMYGLKGAAMANDEGIYPM
                     ITKNGIKVMSINLLLPTEDTPVIWRGPVLANMVKQFWTDVIWGDVDYLFVDMPPGTGD
                     VPLTAFQSLPIEGIVIVTSPQDLVKMIVKKAFNMAEMMKIPVLGIVENYSYVKCPDCG
                     KEIKVFGESHIDEIAAELKVPVLGKMPIDMDYATKADGGFFAAIDNQ"
BASE COUNT          751 a          392 c          538 g          611 t
ORIGIN      
        1 gacagtctga aaaacgtctt ttgaattctt ttccgatttc attgatcaga gtaatatcca
       61 tctggtgatt cagaaaactg tctactttta aaatatttcc cggttttaca actccatcct
      121 taagaattcg gtcttttaaa agcttcatag atttctctcc tttatatatg taattagttt
      181 gtagctgtcg gtaactatcc ggtacactgc ttcccgaaag tactgaatag ttacagttac
      241 tgtgaatatg cagaattcaa aaaaacatca ggctgcaaaa tgaacagtaa cattgctgtt
      301 ttgataatta tagactatga tacgctataa ttcgtcttca tgcaattatt tttgacaaaa
      361 aatctaaaaa taattataat agtctcagac tggttgggaa aaaccgcagg acaatctgtg
      421 cagattgccg taaatcagcc gaagagggct aaatagtgaa acggattgtg aatatccgtc
      481 ttacggactg gagatggaat atgaaagaat atgaactgat atgggaaatt tttaataaat
      541 gtccgcggaa tcagatgcgt gatgtttttg tggaagagct ggaacttgag gatccggaag
      601 aatatatcag aaataaattc aaaggaaaag aagtttccta tgaaaaaact gtcctggagg
      661 atggaaccat tatttttgat attacgactt cacaaataaa acagcgttgc tcctttacag
      721 aaatttgaca aatgtggtat aatatcattt acgctgtacg gaagtgctgt aagcaggaaa
      781 gccacagaca gtgtgtgagg atataaagac agataagcgg taaagaatcg agagaaaggg
      841 gaatgttatg gccgaggaac aaaagacaca tacgcatgta ctggaggatg ggacggtagt
      901 tgaacattct catggggagc atgggcatca tcacagtcat gcgcatacaa aggcagtgct
      961 gaatcgtatg tccagagcaa tcggacatat ggaatctatc aaacgtatga tagaggacgg
     1021 aagagactgt gcggaagttc tgatacagct ttcagcagtg aaatctgcta tcaataatac
     1081 aggtaagatc attctgcagg atcatatcga acactgtatc gtagatgcag tggaacatgg
     1141 tgataaagag gcaattaaag aacttgaggt agcaattgac agatttgtaa agtaatatgc
     1201 gtactctgga gcttgtttga aaaatgtttt acgcaagatg tgcgtcacaa tttgtgggaa
     1261 tgatttttca gacacgctcc acactggcaa tagagaatat tttgttattg ttcacacacc
     1321 actatcccgc gaggtgtttt gacatgaata tgccaaaatc ccgaggcata caagccaaaa
     1381 ttccattgcc gtaggcaatc tggaatgaat tttggctacg ttactgtgaa cggagtgaac
     1441 agtaacaata ttttgtcagt acagcagtgc gtaaatataa aggagaagat taattatggc
     1501 aaaagaatgt aacaacactt catgcgacaa agcaagctgc gaaggttgtt ccagcagcaa
     1561 gaaacagcag agctttcagg cagaaatgaa tgctcagtca aatgtaaaac atgtgatcgg
     1621 ggttgtcagc ggaaagggtg gagttggtaa atccttcgta acaggctctc tggcaaacat
     1681 gatggcagca cagggataca aagtcggtat cttggatgcg gatatcacag gaccgtccat
     1741 tccgaaaatg tatggattaa agggtgcagc aatggcaaat gatgaaggta tctacccgat
     1801 gatcacaaaa aacgggatca aggtaatgtc catcaacctt ctgcttccta cagaggatac
     1861 tcctgtaatc tggagaggtc cggttcttgc aaatatggta aaacagttct ggacagatgt
     1921 tatctggggc gacgtagact atctgtttgt ggatatgcct cctggaacag gtgatgttcc
     1981 gcttacagca ttccagtctc tgccgatcga aggaatcgtg atcgtaacat cacctcagga
     2041 tctggttaag atgatcgtta agaaagcctt taatatggct gagatgatga agataccggt
     2101 acttggaatc gtagagaact acagctatgt aaaatgtccg gactgcggaa aagagatcaa
     2161 agtctttggt gagagccata ttgatgagat cgcagcagag ctgaaggttc ctgtacttgg
     2221 aaaaatgccg attgatatgg attatgcaac caaggcagac ggcggattct ttgcagcaat
     2281 cgataatcag ta
//
LOCUS       DBMDXG010000361         2289 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9912_length_2289_cov_1.794790, whole genome shotgun sequence.
ACCESSION   DBMDXG010000361 DBMDXG010000000
VERSION     DBMDXG010000361.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9912_length_2289_cov_1.794790"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="panC"
                     /locus_tag="ACZPD3_09095"
     CDS             complement(<1..570)
                     /gene="panC"
                     /locus_tag="ACZPD3_09095"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR1416178.1"
                     /translation="MQVTKTVEETRKQIKAWKKEGKTIGLVPTMGFLHEGHASLIKKC
                     REQNDIVVVSDFVNPTQFGPTEDLEAYPRDFERDSKLCESLGTDLIFCPEPSEMYHDP
                     HAFVSIDTLSETLCGKTRPIHFKGVCTVVTKLFHIVAPDRAYFGQKDAQQLAIIRKMV
                     QDLNFDIEIVGCPIVREEDGLAKSSRNTYL"
     gene            complement(680..1072)
                     /gene="panD"
                     /locus_tag="ACZPD3_09100"
     CDS             complement(680..1072)
                     /gene="panD"
                     /locus_tag="ACZPD3_09100"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR1416179.1"
                     /translation="MTIEMLKGKIHRATVIQAELDYVGSITVDEELLEAAGILEYEKV
                     QIVDVNNGSRFETYTICGKRGSGMICLNGAAARCVSTGDKIIIMAYAGYEPEEARTHK
                     PAVVFVDEENKISRVTNYEKHGLLKDMA"
     gene            1902..2033
                     /locus_tag="ACZPD3_09105"
     CDS             1902..2033
                     /locus_tag="ACZPD3_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416180.1"
                     /translation="MQREDFSNRRNYEREFRTVSANIDMILGKSYEQEQQIEKEQNL"
     gene            2098..>2289
                     /locus_tag="ACZPD3_09110"
     CDS             2098..>2289
                     /locus_tag="ACZPD3_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6219 family protein"
                     /protein_id="HIR1416181.1"
                     /translation="MKAHKYWSLGALAAMIGTFYTGYKNMKTAHKYFACSSLLCMIMA
                     IYSGHKMISGKSRKKKNSVS"
BASE COUNT          644 a          499 c          404 g          742 t
ORIGIN      
        1 taaatatgta tttctggagg attttgccag tccgtcctct tcacggacaa tcgggcagcc
       61 tacgatttct atatcaaaat tcagatcctg gaccattttg cggatgatgg ccagctgctg
      121 agcatccttc tgtccgaaat atgctctgtc cggggctaca atatggaaca gcttggttac
      181 aactgtgcac acacctttga aatggatagg acgtgttttt ccacaaagtg tctcagataa
      241 tgtatcgata cttacaaatg catggggatc atgatacatt tcagaaggtt cagggcagaa
      301 gatcaggtct gtgccaagac tttcacagag tttgctgtcg cgttcgaaat ctcttggata
      361 tgcctcaaga tcctctgtcg gaccaaactg tgttggattg acaaagtcag acacaactac
      421 aatgtcgttc tgttctctgc attttttgat cagacttgca tgtccctcat gaagaaatcc
      481 cattgttgga acaagaccta tagttttacc ttcttttttc catgctttga tctgttttct
      541 tgtttcttct actgtttttg ttacctgcat tgttatctcc tctttctcgg tcaatggacc
      601 ggtatcatag attttgagat agcctctttt ctgaattcac tttcatctta tgatagcctc
      661 taatctgata tcaacgtttt caggccatgt ctttcagcag accatgtttc tcataattgg
      721 tcactctgct gatcttattc tcttcatcta caaatacaac tgcaggctta tgagtgcgtg
      781 cctcttcagg ctcatatcct gcgtaagcca tgatgataat cttgtcacct gtgcttacac
      841 atcttgctgc tgctccgttc agacagatca taccgcttcc gcgttttccg cagattgtgt
      901 atgtctcgaa tcgactgcca ttgttcacat ctacaatctg aactttctca tattccagta
      961 ttccggcagc ttccagcagt tcttcatcca cagtaatgct tcccacataa tccagttcag
     1021 cctggataac agttgctctg tggatttttc cttttaacat ttctattgtc atcttatata
     1081 gtcctttcct gcgggaaaaa tcagagtcaa gcagcaccat aaaaaggaaa tataccttta
     1141 tgatctgcaa aaaaagcgca tcaggataca ctttgaccct gaaacgcttc tgattgattc
     1201 cctgtatatt gagtcctgtt tctgatacat aaaatatgtt ttactattcc atagattatt
     1261 ggaaatctga ttttttgtat cataaatacc ggctcgtatc ttttaaaatt acatacatcc
     1321 ctccgttccc gtttccggat tccggctggc tgcttttata taagattcca gtgatttcag
     1381 ccagagtata gttttgagtc caaatactat ctctgagtgg tattattaca tctggtcagt
     1441 tatttgtcaa taaattgttt atttactggt tactgttcac tccgcgtcca gtaatacact
     1501 gcgaaataca ataaatatga atctccttgt gcaaagccca aggagtatcg tttgcttcgc
     1561 agacttaatt tatgctaatc acataaggaa ccaaaaaatc actcatctga caaacaatca
     1621 gacggatagc ctcatccaac cgtaccttcc cgggagttat ttccaattcg ttcatatgtg
     1681 caaagaaatt tttccgaaag acttcatacg gcatcaataa ctctgccgca aacaggtttg
     1741 taatctgaat gtcagaaact aaacagctat taccataaaa ataaaaagtt tctctttatg
     1801 atacagctct ccgaacactg aaagaaaaat ctgtcggaaa taagctgcct tccacgaaat
     1861 ccctctatgc tgaaaaagac cgacttattg aattgcgaaa tatgcaacgg gaagattttt
     1921 ctaaccgccg gaattatgaa cgggagtttc gtaccgtgtc tgccaatatt gatatgatcc
     1981 ttgggaagag ctacgaacag gaacagcaga ttgagaaaga acagaattta taaatttcct
     2041 gttttcatgc tttctaaaaa gaggtattat ataaaaaaac gcaggaggta tattcaaatg
     2101 aaagcacata aatattggtc acttggtgca ttggcagcca tgattggaac cttttatacc
     2161 ggctataaaa acatgaaaac tgctcacaaa tattttgctt gcagctctct tctctgcatg
     2221 attatggcta tctactccgg tcataaaatg atttctggta aatccagaaa aaagaaaaat
     2281 tctgtttct
//
LOCUS       DBMDXG010000362         2278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9964_length_2278_cov_2.045935, whole genome shotgun sequence.
ACCESSION   DBMDXG010000362 DBMDXG010000000
VERSION     DBMDXG010000362.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9964_length_2278_cov_2.045935"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="ACZPD3_09115"
     CDS             complement(<1..572)
                     /locus_tag="ACZPD3_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIR1416182.1"
                     /translation="MNLDKLFHLKENHTDVKTEVMAGITTFMTMAYILAVNPNILEAS
                     GMDRGAVFTATALSSFIATCLMALLSNYPFVLAPGMGLNAYFAYTVVLGMGYNWQQAL
                     AAVFVEGIIFILLSLTNVREAIFNAIPMNLKHAVSVGIGLFIAFIGLQNAKIVVNNDS
                     TLVSVFSFKSSVSGGTFNTEGITVLLALIGL"
     gene            complement(735..2012)
                     /locus_tag="ACZPD3_09120"
     CDS             complement(735..2012)
                     /locus_tag="ACZPD3_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR1416183.1"
                     /translation="MNERTFVLKGTICYSNSLTELSITENGYLVCEDGRCAGVFDELP
                     EKFAGISCTDFGDELIIPGLTDLHLHAPQYTFRASGMDLELLDWLNTYTFPQEARYED
                     TEFAKEAYSIFTEDMKKSPNTRACIFGTLHVPATEILMEQLDKTGIKAMVGKVNMDRN
                     GSPQLQEESAQASADATVQWIKDTLDKFENVKPILTPRFTPSCSDALMEKLSEIQKKY
                     HLPMQSHLSENFGEIAWVKELCPNTHFYGEAYSQFDLFGGDCPTIMAHCVHSTDEEIA
                     LMKKQGVYIAHCPQSNTNLASGISPARRYLDEGLHIGLGSDIAGGTSVSILRAMADAI
                     QVSKLYWRLVDLSMKPLTVEEAFYMGTEGGGSFFGKVGSFKEGYEFDAVVLNDSTIPT
                     PLKLSPKDRLERLIYLSDDRNITAKYVAGRKIL"
     regulatory      complement(2084..2179)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT          673 a          515 c          455 g          635 t
ORIGIN      
        1 agtccgatca atgcaagcag aacagtgatt ccctcagtat taaatgttcc gccggatacg
       61 gaagatttaa aggaaaatac gcttaccaga gtagagtcat tgttaactac gatctttgca
      121 ttctgcagtc cgataaaagc aatgaaaaga ccgataccga cggatactgc atgtttcagg
      181 ttcattggaa tagcgttaaa aatcgcttca cgcacattag tgagtgataa aaggataaaa
      241 ataatacctt ctacaaatac agctgccagt gcctgctgcc agttgtatcc catgccaaga
      301 acaactgtgt atgcaaagta ggcatttaag cccatgcccg gtgcaagaac gaacgggtag
      361 ttagaaagca gtgccatcag gcaggtggca ataaaggatg acagtgcggt cgctgtaaaa
      421 acagctcctc tgtccatgcc ggacgcttcc agaatgtttg ggtttacagc cagaatgtat
      481 gccattgtca tgaatgtggt aatacctgcc ataacttcgg tctttacatc tgtgtggttc
      541 tctttgagat ggaataattt gtcaagattc ataatctttc ccccttttga ttttaaaaat
      601 tgccggctgt tttgtggtgt ctcagataat catcccgaaa aaacagggtg aaactcctgg
      661 aatcaatcgt ctcggcatat cgttttgtta acgatatgcc ggaaaaacac catcagaatc
      721 tgtatttcct gcagttacag aatctttctt ccggctacat atttagcggt aatattgcgg
      781 tcatcggaaa ggtagatcag tctttcgagt ctgtccttcg gagagagctt cagtggagtt
      841 ggaatagtgc tgtcattaag cacaaccgca tcaaattcat acccctcctt aaagcttccc
      901 acctttccaa agaaggaacc gcctccttct gttcccatat aaaaggcttc ttcaacagtg
      961 agcggcttca tggaaagatc aaccagccgc cagtacagct tggaaacctg aatagcatca
     1021 gccatggcac gcagaataga aacggaagtt ccacctgcaa tgtcagagcc cagtccgatg
     1081 tgtaagcctt cgtccagata acgtctcgca ggagagatac ctgaggcgag atttgtgttg
     1141 gactgcggac aatgagcaat ataaacgccc tgctttttca taagggcaat ttcttcatcc
     1201 gtagaatgaa cacaatgagc cataattgtc ggacagtcac cgccgaaaag atcaaactgg
     1261 ctatatgctt ctccataaaa atgtgtattc gggcaaagct ccttcaccca ggcaatttca
     1321 ccgaaattct cagaaaggtg agactgcatc ggcaggtgat actttttctg aatctccgaa
     1381 agcttctcca tcagtgcatc agaacaggac ggagtaaatc tcggagtaag gatcggcttt
     1441 acattttcaa atttatcaag cgtatcctta atccactgaa ccgtagcatc agcagaagcc
     1501 tgagcactct cttcctgcaa ctgcggagaa ccattacggt ccatatttac cttgccgacc
     1561 atagccttta ttccggtttt atccagctgt tccatcagga tctcggtagc agggacatgc
     1621 agagttccga aaatacatgc tcttgtattc gggctcttct tcatatcctc agtgaaaata
     1681 gaataagcct cttttgcaaa ctctgtatct tcataacgtg cttcctgcgg aaaagtataa
     1741 gtattcagcc agtccagcag ctcaagatcc atacccgagg cacgaaacgt atactgcgga
     1801 gcatgaagat gcagatcagt cagtcccgga atgatcagtt catcgccaaa atctgtacat
     1861 gagattcctg caaatttctc aggaagctca tcaaacaccc ctgcacatcg cccatcttca
     1921 catactaaat atccattttc agtgatggaa agttctgtca gagaattact gtagcaaatt
     1981 gttcccttta aaacaaaagt ccgttcgttc ataaccatac ctcctttttg tggttaagga
     2041 aaggcaaaaa aaatctaccg atgctgcctt tacctcattc tttctccctg gggagagaac
     2101 ttatagacaa ctattacggt agttggtaga aacgtccaac cgattatgga catatataag
     2161 tttacttata ttgtttataa aataattata aaatatttgt agttaactgt caattacttt
     2221 ctgcaattta atttaatatt ctttttgcat tgctatttga taattcatag tctataaa
//
LOCUS       DBMDXG010000363         2277 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9972_length_2277_cov_1.751379, whole genome shotgun sequence.
ACCESSION   DBMDXG010000363 DBMDXG010000000
VERSION     DBMDXG010000363.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9972_length_2277_cov_1.751379"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            568..1512
                     /gene="nikB"
                     /locus_tag="ACZPD3_09125"
     CDS             568..1512
                     /gene="nikB"
                     /locus_tag="ACZPD3_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HIR1416184.1"
                     /translation="MRKYAFRRLLQLIPILFAITFLSYGMMRIAGSDVVTQKMENTGQ
                     ILSEDKLNAAREQLGLDKPFLTQYFVWLGKLLHGDMGNSYVSSLPVFDTFISKLPATL
                     LLTVTSILLTIIISIPLGILSAVKQNTITDYLIRLCSFIGNSLPNFFVSLLLMYFLAI
                     RLRIFPVISKDVSLKSVAMPAITLAIAMSAKYLRQVRATVLDELSKDYVAGAKARGVK
                     FSVTLWKSIMKASLVTIITLLMLSVGNLLGGTAIVESIFMWDGVGKMAVDAISMRDYP
                     IIQAYVMWMAIIYVVVNLLTDLSYRFLDPRIRLGGVKQ"
     gene            1509..>2277
                     /locus_tag="ACZPD3_09130"
     CDS             1509..>2277
                     /locus_tag="ACZPD3_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1416185.1"
                     /translation="MSEKQNKNVTVRVQKIKKNHIKQKLIFFLILAIGLVILAIFSEH
                     LCPYDPYAQDLASALQPPSLQHPMGTDTYGRDMLSRVISGARASIFSTFILVAVIAVL
                     GTAVGVCCGYFGGAADAVMMRISDVCLAFPGLVFAMAIAAILGGGIQNAVIALAVVSW
                     PKYSRIARGQTLALKEEPYIHAAILAGDSSGQIMLRHILPNMLGPVLVTAMLDIGTMM
                     MELAGLSFLGLGAQIPMAEWGSMMSSGRSMIQTYPWVV"
BASE COUNT          629 a          420 c          550 g          678 t
ORIGIN      
        1 gagaagaaat ggatgagagt gtggcagagc ttgtggagtt tttaaagagc agactgccga
       61 agcattaata aaaattgcag ttgacttttt aacttatttt gcatatacta atagtagcta
      121 agaaatgaag tgaaatttcc atgaagcgaa acgaaagccc tcagagttgc cgctctgagg
      181 gctttcttgc tcgttacggg gagctgttcc gtttagactg ttgctgtcta atgtccgctg
      241 tccagccatt tgcatatgta gtagctaact acgcctgcta tgacagagac aagaaatgaa
      301 gtaaaaattg cttccatgaa actcacctcc ttcctgctgg aggttcgaca gctcttgtag
      361 tataacatag tttagaacaa aatgggatgg ttgttttaaa ttattgctga tattaaaaaa
      421 acctattccc aaaatgacga tagatattca aattaagaat agcttataac ccccagggtg
      481 ggttgtgggc tatttttgct tttgttaaac tgaacggtgg caaattagga gtacaatttt
      541 caaaaattat aaaatggaga taatcaaatg agaaaatatg cattcagacg actgctccag
      601 ctgattccca ttttgtttgc gattactttt ctctcctatg gcatgatgcg gattgcggga
      661 agtgatgtag tgacacaaaa aatggaaaat acaggacaga tcctgtcaga agataagtta
      721 aatgcagcca gagaacagct gggactggac aaaccatttc tgacccagta ttttgtgtgg
      781 cttgggaaat tgcttcatgg agatatggga aacagctatg tatccagtct tccggttttt
      841 gatacgttta tatcgaagct tccggcaacg ctgcttctta cagtgacatc tattttgctg
      901 actatcatca tatcaatccc tctggggatt ctgtcagcag taaaacagaa tacgatcacg
      961 gattatctga tccgtttatg cagctttata ggaaacagtc ttcctaattt ttttgtatca
     1021 ttgctgttaa tgtattttct tgcgatccgg ttgcggattt ttccggttat atccaaagat
     1081 gtaagtttaa agagtgtggc aatgccggcg atcactctgg caattgccat gtcagccaag
     1141 tatctccgcc aggtccgtgc aactgtgctg gatgaattaa gcaaagacta tgtggcagga
     1201 gcgaaagcaa gaggagtaaa gttttctgtc actctttgga aaagtattat gaaggcttcg
     1261 ctggtaacca tcattacctt gctgatgctg tcagtgggaa atctgctggg aggaactgca
     1321 atcgtagaat caatctttat gtgggatgga gttggaaaga tggcagtaga tgccatctcc
     1381 atgcgtgatt atcctattat tcaggcctat gttatgtgga tggcgatcat ctatgttgta
     1441 gtaaatctgc ttacagatct gtcataccgg ttcctggatc cgagaatccg tctgggaggt
     1501 gtgaaacaat gagtgaaaag cagaataaaa atgtgacagt gcgtgtacag aagattaaga
     1561 aaaaccatat aaaacaaaag ctgatctttt ttcttattct tgcaataggg cttgtaatac
     1621 ttgcaatttt ttcagagcat ctctgtccat atgaccctta tgcacaggat ctggcaagtg
     1681 cacttcagcc ccccagtctt cagcatccta tgggaacaga tacatatggg agagatatgc
     1741 tgtccagagt gataagtggt gcaagagcca gtattttctc tacctttatc ctggttgctg
     1801 tgatcgctgt gctgggaaca gctgtgggtg tatgctgcgg atattttggc ggagctgcag
     1861 atgctgttat gatgaggatt tcagatgtat gtcttgcttt ccctgggctt gtatttgcca
     1921 tggcgatcgc ggctattctt gggggaggca ttcagaatgc tgttatagca ctggcagtag
     1981 taagctggcc gaaatattca cgtattgcca gagggcagac tctggcatta aaagaggaac
     2041 cctatattca tgctgccata cttgcaggtg acagctcagg tcagattatg ctcaggcata
     2101 ttcttcccaa tatgctggga cctgttctgg taacagccat gcttgatatc ggtaccatga
     2161 tgatggaact tgcaggtctg tcatttcttg gactgggagc acagattccc atggctgagt
     2221 ggggaagtat gatgagtagc ggacgaagta tgatccagac atatccatgg gtagtat
//
LOCUS       DBMDXG010000364         2274 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_9993_length_2274_cov_1.477220, whole genome shotgun sequence.
ACCESSION   DBMDXG010000364 DBMDXG010000000
VERSION     DBMDXG010000364.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9993_length_2274_cov_1.477220"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="ACZPD3_09135"
     CDS             complement(<1..265)
                     /locus_tag="ACZPD3_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR1416186.1"
                     /translation="MDSLQLPDSLEVLDESAFFKCCNLTSVVIPPNVRYLGKWIFHGC
                     NRLQYLEIRHDPEFIGEWIINKAATIRCYKGSKVDRYCQQSGFK"
     gene            complement(301..804)
                     /locus_tag="ACZPD3_09140"
     CDS             complement(301..804)
                     /locus_tag="ACZPD3_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HIR1416187.1"
                     /translation="MSHSLTLTRRNCGQYIEKNMELPQNCRAISAETFFNRPDIRSVV
                     FPYEMEQIGNKAFQGCSRLSRIELPEHLQKLGIGVFSDCISLKKVKIPASLTSIPKNA
                     FNNGRKLNSLEFAPNSKLTFIGPNAFSECATLTEVVFPASLEEIDDRAFYKCKKLSCI
                     SFPGGGI"
     regulatory      complement(1008..1210)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1714..>2274
                     /locus_tag="ACZPD3_09145"
     CDS             1714..>2274
                     /locus_tag="ACZPD3_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416188.1"
                     /translation="MNNTIFDDVFRTMVEKIPRLVIPLINEVFHTSYPEDVEITQLRN
                     EHQQADGEVITDSCLLIGKKMYHIECQSTDDTTMAVRMIEYDFAIAIEYAAKQGRRYE
                     IEFPRSCVLFLRSSGNTPDFLETNVIFPDGRKQMYRVPTVKMADYTAESIFEKNLLML
                     LPFYIMRYEKRAHDMRENPRLFQTLLN"
BASE COUNT          671 a          415 c          528 g          660 t
ORIGIN      
        1 ctttgaatcc tgactgctgg cagtatctgt ctactttaga acctttataa cagcggatcg
       61 tagccgcttt gttgatgatc cattccccga taaattcagg atcatggcga atctccagat
      121 actgcaggcg gttacatcca tgaaagatcc attttccaag gtatctgaca ttgggcggaa
      181 tcaccacaga ggtcagatta cagcacttaa aaaaagcact ctcatcaaga acttccagcg
      241 aatcaggcag ctgcagcgaa tccattccgc agaaatagaa agcctgcttt ccgataaccc
      301 ttaaatcccc ccccccggaa aagaaataca actcagtttc ttacatttat aaaaagccct
      361 gtcatcaatt tcttccagag aggcagggaa aacaacttcc gtaagagtag cgcattctga
      421 aaaggcattc ggaccgataa atgtcagttt gctgttcggg gcgaactcca gactgttcag
      481 tttccggcca ttgttaaatg cgttttttgg aatactggta aggctggcgg gaattttgac
      541 ttttttcaga gaaatgcagt ctgaaaatac ccctatgcca agtttttgca gatgttcagg
      601 aagctctatt ctgcttaacc gggaacatcc ctgaaaggct ttgtttccaa tctgttccat
      661 ttcataggga aataccacag aacgaatatc aggtctgtta aaaaaggttt ctgctgagat
      721 agcgcggcag ttttgtggaa gttccatatt cttctcaata tactgtccgc agttacgacg
      781 cgtaagtgtc agagaatgtg acataaaaaa atactccttt gctgatagac aatgttgttt
      841 tctctgtatg ggagtagtat ggactatgat aaaaataaca aggtaccgat aactgccggt
      901 gttctatatt ccttaaaagg aacagagaaa acatatgtaa ggatgatact tctgtaaaaa
      961 agagaacact aaagaagatc acccttccat ttcctattgc gtgtagttaa tggcggcagt
     1021 ttatagcagg tattctgact cacgcagtaa cgccatgtct ctccttctcg cataacgatg
     1081 caatggatat gagatatggc tgcacgtata cagcagcacg actgttcagg attctgacct
     1141 gattccctct tagctgaaag tccgacattt ctataccgtg actgacagaa ctaaaaaccg
     1201 gtaattgtca atgaatgttc gattaaattg tattatacac ttttttggag aaaagacaac
     1261 aaattttttg agggggagac aaaatcaggg aggggacgcg cagggaaagc gtttcttttc
     1321 tgtgcgctgg ttattggatg tgtggggatt tgtatgattt gcaggtaatg tttttggttt
     1381 tgaaatagta gttgaatagt aattgaacag taatgtgtga aatagtgctt gatttttgaa
     1441 acagcgtatt ttagactata gattatagtg aaaatcttat attactcgtg ccaggtctgg
     1501 cggttcataa cgggtttctc tggcgttcgc cgacattttc acaaagataa tttaaatttt
     1561 atagtggtaa agcaaacgtt cctgaatatt ataacattct gcccgttggc tttgcctgaa
     1621 cgggaggtgg gaataaaaca gttcatggtt gattaagggt tgttttggag gttctcaaag
     1681 ctctgggaaa atgttataat aaggaggaat taaatgaaca atacgatttt tgatgatgtg
     1741 ttccgcacaa tggttgaaaa gataccacgg ctggttatac cattgatcaa tgaagtgttt
     1801 catacatcct atccggaaga tgtagagatc acgcagcttc gcaatgagca ccagcaggcg
     1861 gacggggagg tcatcacgga ttcctgtctt ctgatcggga agaagatgta tcatattgaa
     1921 tgtcagagta cggatgatac gacaatggca gtgcgtatga tagagtatga ctttgcgatt
     1981 gctattgaat atgcggcaaa gcagggacga aggtatgaga tagagttccc gagatcctgc
     2041 gtgttgtttt tgagaagttc ggggaatacg ccggattttc tggaaacgaa tgtgattttt
     2101 ccggatggga gaaagcagat gtaccgcgta ccaactgtaa aaatggctga ttatacagcg
     2161 gaaagtattt ttgagaaaaa tctgctgatg ctgcttccgt tttatatcat gagatatgag
     2221 aaaagagccc atgatatgag ggagaatccc cggttatttc agacattgtt gaat
//
LOCUS       DBMDXG010000365         2266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10030_length_2266_cov_1.893303, whole genome shotgun sequence.
ACCESSION   DBMDXG010000365 DBMDXG010000000
VERSION     DBMDXG010000365.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10030_length_2266_cov_1.893303"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            83..979
                     /gene="rfbA"
                     /locus_tag="ACZPD3_09150"
     CDS             83..979
                     /gene="rfbA"
                     /locus_tag="ACZPD3_09150"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR1416189.1"
                     /translation="MKGIILAGGSGTRLYPLTMVTSKQLLPIYDKPMIYYPMSVLMNA
                     GIRDILIISTPQDTPRFEELLGDGHQFGVHLTYAVQPSPDGLAQAFIIGEEFIGNDTV
                     AMVLGDNIFAGHGLKKRLKAAVKNAESGEGATVFGYYVDDPERFGIVEFDHEGKAISI
                     EEKPEHPKSNYCVTGLYFYDNRVVEYAKNLKPSARGELEITDLNRIYLEKGELNVELL
                     GQGFTWLDTGTHESLVEATNFVKTMESHQHRKIGCLEEIAYLNGWITKEDVLKVYEIL
                     KKNQYGQYLKDVLDGKYLDVLH"
     gene            1156..2184
                     /gene="rfbB"
                     /locus_tag="ACZPD3_09155"
     CDS             1156..2184
                     /gene="rfbB"
                     /locus_tag="ACZPD3_09155"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR1416190.1"
                     /translation="MNIIVTGGAGFIGSNFIFHMLKKYPDYRIICLDCLTYAGNLSTL
                     APVMDNPNFRFVKESITDREAVYKLFEEEHPDMVVNFAAESHVDRSIENPEVFLTTNI
                     IGTAVLMDACRKYGIKRYHQVSTDEVYGDLPLDRPDLFFTEETPIHTSSPYSSSKASA
                     DLLVLAYHRTYGLPVTISRCSNNYGPYHFPEKLIPLMIANALNDKPLPVYGTGENVRD
                     WLYVEDHCRAIDLIIHKGRVGEVYNVGGHNEMTNIDIVKIICKELGKPESLITYVADR
                     KGHDMRYAIDPTKIHNELGWLPETKFADGIKKTIKWYLDNKEWWETIISGEYQNYYEK
                     MYANRGEA"
BASE COUNT          710 a          501 c          485 g          570 t
ORIGIN      
        1 gttttgctta tcatttacac agatagataa ctgacaaaat atcgctccga ccgcatactg
       61 ccaataacaa aggagaatac acatgaaagg tattatttta gcaggcggtt ccggcacacg
      121 cctttatccg cttacaatgg taacatccaa acagcttctg ccaatctatg ataaaccaat
      181 gatctactat cccatgtctg tactgatgaa tgcagggatc cgcgatatcc tgatcatctc
      241 tactccgcag gatactcctc gttttgagga acttcttggt gacggacatc agttcggtgt
      301 ccatctcacc tatgcagtac agccaagtcc ggacggactt gcacaggcat ttatcatcgg
      361 tgaagaattt atcggtaatg atacagttgc catggttctc ggcgataata tcttcgcagg
      421 acatgggctg aagaaaagac tgaaagctgc tgtaaagaac gcagaatccg gcgagggtgc
      481 aactgttttc ggatactatg tagatgatcc ggagagattc ggtattgtag aatttgacca
      541 tgaaggcaag gctatctcaa ttgaagagaa accggagcac ccgaagagca actactgtgt
      601 cacaggttta tacttctatg acaatcgtgt cgtagagtat gcaaaaaatc tgaaaccaag
      661 tgccagaggc gaacttgaga tcacagatct gaaccgcatc tatctggaaa agggcgaatt
      721 aaacgtagag cttctcggac agggattcac atggctggat acaggtacac acgagagcct
      781 tgtggaagcc accaactttg taaaaacaat ggaaagccac cagcaccgca aaatcggttg
      841 cctggaagag attgcatatc tcaatggctg gattacaaag gaagatgtgc tgaaggttta
      901 tgaaatactg aagaaaaatc agtatggtca gtacttaaaa gacgttctgg acggtaaata
      961 tctggatgta ttgcactaac cgaagctttt agacctgtac aagcgaattc gcagtcaagt
     1021 gagtgatata tcaaattttc aaacagttat ttgatatatc acccgccatg cagcaaggcg
     1081 gataatttta tctgcctgac tctataacag aatcttacag atattcatat tatatatttt
     1141 ttaaggagaa tcactatgaa cattatcgta accggcggtg ccggatttat cggaagcaac
     1201 tttatttttc acatgttaaa aaaataccca gattaccgca tcatctgtct ggactgtctg
     1261 acctatgcag gaaacctttc cactcttgca cctgtcatgg acaacccaaa tttccgtttc
     1321 gtaaaagaaa gcatcaccga ccgcgaagca gtttacaaac tttttgagga agaacaccct
     1381 gatatggttg taaacttcgc tgcagagagt cacgttgatc gctccatcga aaacccagaa
     1441 gttttcctta ccacaaatat tatcggtact gctgttctca tggatgcatg ccgcaaatac
     1501 ggtatcaaac gttatcatca ggtatctact gatgaagttt acggagatct tccattagac
     1561 agacctgacc tgttcttcac agaggaaact ccgatccaca caagcagccc ttacagctct
     1621 tccaaggctt ctgcagacct tctggtgctg gcttaccaca gaacctacgg actgcctgta
     1681 accatcagcc gctgctccaa taattacgga ccatatcact tcccggaaaa actgattccg
     1741 ttaatgatcg ccaatgcatt aaatgacaaa ccacttccgg tatacggaac aggcgaaaac
     1801 gttcgtgact ggctctatgt agaagatcat tgccgcgcaa tcgacctgat catccacaaa
     1861 ggacgtgttg gagaagttta caacgtcggc ggacacaatg aaatgacaaa cattgatatc
     1921 gttaagatca tctgcaaaga acttggcaaa ccagaaagcc tgatcaccta cgtagcagac
     1981 cgtaaaggtc atgacatgcg ttatgcaatc gacccgacta agattcacaa cgaacttggc
     2041 tggttaccgg aaacaaaatt tgcagacggt atcaagaaaa caatcaaatg gtatctggat
     2101 aacaaagaat ggtgggaaac aatcatctcc ggtgaatacc agaactacta tgaaaaaatg
     2161 tacgcaaacc gcggggaggc ataatttatg agaatttttg taacaggtgt aggcggtcag
     2221 cttggacatg atgttactct gaacggctct gaaggtgtgt aatcat
//
LOCUS       DBMDXG010000366         2261 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10063_length_2261_cov_1.697222, whole genome shotgun sequence.
ACCESSION   DBMDXG010000366 DBMDXG010000000
VERSION     DBMDXG010000366.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10063_length_2261_cov_1.697222"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(181..2208)
                     /gene="ligA"
                     /locus_tag="ACZPD3_09160"
     CDS             complement(181..2208)
                     /gene="ligA"
                     /locus_tag="ACZPD3_09160"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007037890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR1416191.1"
                     /translation="MKQNQNEHLSQEAIRIHHLVKQLNQYRHEYYNMNAPSVSDAVYD
                     CLFDELDALEKRTGIILSNSPTQTVGYQAVSQLDKVHHPIPLLSLDKTKQITEIHDFM
                     KKQKSLLMLKLDGLTIKLTYENGRLIEASTRGDGDEGEIVTHNASAFRNIPLSIPYQK
                     RLVISGEAFIHIHDFERLQNTLLDSNGNPYRNARNLAAGSARCLDANICKERSVSFYA
                     FHVLEGLDESSELSKSREARLTALHPLGFDICPFLVLSSEASLETLNEAMATLRDLAS
                     ELDIPIDGLVLRYDDLPYSVACGRTGRFYKDGKAFKFEDDTFKTIFRSIEWQTGRTGE
                     IAPVALFDPIEIDGSTVSRASLHNLTFIQDLELYPGCRILVSKRNMIIPHIEENLDRG
                     HYQDMTPRTCPCCGSPTRIYSRSSGSERIVQTLHCDNPNCSQQILQRFVHFCEKKAMN
                     IIGISEATLKQFIDLGFLKCFQDIYHLDRFSDQIADMDGFGKKSYERLWNSINESRNT
                     TFVRYLVAMDIPMIGRTASRKLEQYFHGNLLELELAAMSRFDFTCLEDFGETMSSNII
                     EWFHDRENLTLWRNLQKEMHFKEREETIMTETKNNPFAGCTIVATGKLENFTRDSINS
                     KIISLGATAGSSVTKKTDYLICGEKAGSKLAKAQQLGVKVLSEQEFLNMIA"
BASE COUNT          589 a          460 c          513 g          699 t
ORIGIN      
        1 gaagctgatc gtaaatgatt cttttcagat acataattca gcgctccttt cgttcgatta
       61 tttctattat aacaaaacat ggagagataa tatagtagaa aatagctctc tttacggtat
      121 attaaaagcg ggaagatacc tatgctgtcc cttcccgctt cattttatac gctttcagta
      181 ttatgcaatc atatttaaaa attcctgttc gctcagaacc tttaccccaa gctgctgtgc
      241 cttcgccagt ttgctgccgg ctttttctcc gcagatcagg taatcggttt tctttgtgac
      301 ggagcttccg gcggtggcgc ctaagctgat gatcttgctg ttaatgctgt cacgggtaaa
      361 gttttcaagc tttccggttg ctacgatggt acatcctgca aatgggttat ttttagtttc
      421 tgtcatgata gtttcctctc tttctttaaa gtgcatttct ttttgtaagt ttctccataa
      481 ggtaagattt tcacggtcat ggaaccattc gataatattg ctgctcatag tttcaccaaa
      541 gtcttccagg caggtaaagt caaatcggct catagctgca agctccagtt ccaaaagatt
      601 tccgtgaaag tattgttcca gtttccggct ggcagttctg ccgatcatgg gaatatccat
      661 ggcaaccaga taacggacaa aggtggtatt gcggctttcg ttaatggagt tccagagccg
      721 ttcataggat tttttaccga atccgtccat atcagcaatt tgatctgaaa agcgatccag
      781 gtgataaata tcctgaaagc atttcagaaa gcccaggtca ataaactgtt ttaaggtggc
      841 ttcagaaata ccgatgatat tcatagcttt cttttcacag aagtgaacaa agcgctgaag
      901 aatctgctgg ctgcagtttg ggttatcgca gtgtagtgtc tgtacaatcc gttccgaacc
      961 ggaacttctg gaatagatac gggtaggact gccgcagcag gggcatgttc tgggtgtcat
     1021 atcctgatag tggccgcgat ctaagttttc ttcgatatgg ggaataatca tatttcgttt
     1081 ggataccaga atccgacatc cgggatagag ttccagatct tgaataaatg tcagattatg
     1141 cagacttgct ctggatacag tagatccgtc aatttcaatc ggatcaaaca gtgccacggg
     1201 agcaatctct ccggttcgtc cagtctgcca ttcaatcgaa cgaaaaatgg ttttaaatgt
     1261 atcatcttca aatttaaaag cttttccgtc tttatagaaa cgtccggtac gcccgcaggc
     1321 gacagagtaa ggcaggtcat catatcggag aaccaggccg tcaatgggaa tatccagttc
     1381 agaagccaga tcacggagcg ttgccatggc ttcattcagt gtttcaaggg atgcttctga
     1441 tgaaagtacc agaaaaggac agatgtcgaa tcccagagga tgtaatgctg taaggcgtgc
     1501 ttcgcggctt tttgaaagct ccgaagattc gtccagtcct tccagaacat gaaatgcata
     1561 gaaggaaaca gaacgttctt tgcagatatt ggcatccaga cagcgagcag agccggcggc
     1621 cagatttctg gcgtttcggt aagggtttcc attgctgtcc aatagggtat tttgcagccg
     1681 ttcgaagtca tgaatgtgga taaatgcttc tccacttata acaagacgtt tctggtaagg
     1741 aatagataaa ggaatattcc ggaaagccga agcattgtgg gtaacgattt ctccttcatc
     1801 gccatcgcct cttgtagaag cctcaatgag ccggccattt tcatatgtga gcttgatggt
     1861 aagtccatcc agcttcagca taagaaggga tttctgcttt ttcataaaat catggatttc
     1921 tgttatctgt tttgttttgt caagagagag cagtggtatc ggatggtgta ctttgtctaa
     1981 ctgactgaca gcttgatatc cgacggtctg tgtcggagaa ttactcagga taattccggt
     2041 tcgtttttct aatgcatcta attcatcgaa aagacaatca tagactgcat cggatacact
     2101 gggagcattc atgttgtagt attcgtgccg gtactggttg agctgtttca ccagatgatg
     2161 aatgcgtatt gcctcctggg agagatgttc attttgattt tgtttcatat gcgcctccat
     2221 tattttttta ttgtcattga atgtgtttga aagataattc t
//
LOCUS       DBMDXG010000367         2243 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10163_length_2243_cov_1.823529, whole genome shotgun sequence.
ACCESSION   DBMDXG010000367 DBMDXG010000000
VERSION     DBMDXG010000367.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10163_length_2243_cov_1.823529"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="ACZPD3_09165"
     CDS             <1..211
                     /locus_tag="ACZPD3_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR1416192.1"
                     /translation="ASFVLTQYNNEVYISARAIDEVNVQVMMEKMGGGGHMNIAGAQV
                     KASPDEVERMLKDIIDQEYQEENTK"
     gene            208..654
                     /gene="rplI"
                     /locus_tag="ACZPD3_09170"
     CDS             208..654
                     /gene="rplI"
                     /locus_tag="ACZPD3_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR1416193.1"
                     /translation="MKVILLEDVKALGKKGQIVNVSDGYARNMILPKKLGLEATPKNL
                     NDLKLQKANEEKVAKEVYEAAQAFAKDLETKEIILTLKTGEGGRTFGSVSSKEISEAA
                     KKQLNLDIDKKKLQLPEPIRTLGVTQVPVRLHPKVTGTLKVWVKEA"
     gene            675..2003
                     /gene="dnaB"
                     /locus_tag="ACZPD3_09175"
     CDS             675..2003
                     /gene="dnaB"
                     /locus_tag="ACZPD3_09175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR1416194.1"
                     /translation="MEEALIKRILPHSIEAEQSVIGSMILDRDAILVASEILTSDDFY
                     QKQYGIIFDAMVELCNEGKPVDLVTLQNRLKEKELPPDISSMEYVRDLISAVPTSANV
                     KYYAKIVSEKAVLRRLIKANEEIANTCYLEKENTEIILEEAEKKLFNILQRRNNEEYV
                     PIQQVVLNAINNIEKASKLKGSVTGIPTGFMDLDYKTSGMHPSDLVLIAARPSMGKTA
                     FVLNIAQYMAFRKDVTVAIFSLEMSKEQLVNRLLAMESHVDSQNMRTGNLKDEDWTKL
                     VEGADIIGKSNLIIDDTPGISIAEMRSKCRKYKLEHNLGIIMIDYLQLMSGSGKSDSR
                     QQEISDISRSLKALARELNVPVIALSQLSRAVEQRPDHRPMLSDLRESGAIEQDADVV
                     MFIYRDDYYHKDTEKKDIAEIIIAKQRNGPIGTVELVWLPRYTQFVNMKK"
BASE COUNT          750 a          410 c          533 g          550 t
ORIGIN      
        1 ggcatcgttt gtactgactc agtacaataa tgaagtttat atcagtgcca gggctattga
       61 cgaagtcaat gtacaggtaa tgatggaaaa gatgggcgga ggcggtcata tgaatattgc
      121 cggtgcccag gtcaaggctt ctccggatga ggtggagaga atgcttaaag acatcataga
      181 tcaggaatat caggaggaaa acacaaaatg aaagtaatct tacttgaaga cgtaaaagca
      241 cttggaaaaa agggacagat cgtaaatgta agtgacggat atgcaagaaa catgatcctt
      301 cctaagaaac tggggctgga ggcaactcct aagaatttaa atgatctgaa acttcagaaa
      361 gcaaacgaag agaaggtcgc aaaagaagta tatgaagcag cacaggcatt tgcaaaggat
      421 ctcgagacaa aagaaatcat ccttacactg aaaacaggag aaggcggaag aacctttggt
      481 tccgtttctt ccaaagaaat ctctgaagca gcaaagaaac agttgaatct tgacattgat
      541 aaaaagaaac tgcagcttcc tgagccaatc cgtacacttg gtgttaccca ggtgcctgtc
      601 agattacatc ctaaggtaac aggaactctt aaagtatggg taaaagaagc ataagtcttc
      661 cggagggata aagaatggaa gaagcgctga ttaaaagaat actcccccac agtatagagg
      721 cagagcagtc tgtaatcggc tcaatgatcc tggacagaga tgcgatcctt gtggcatctg
      781 agatacttac cagcgatgat ttttatcaga aacaatatgg catcatcttt gatgcaatgg
      841 tggaattgtg taatgaaggg aaaccggtag atctggtgac ccttcagaat cgtttaaagg
      901 agaaagaact cccgccggat atcagcagta tggaatatgt aagagatctg atctcagcgg
      961 ttcctacttc tgcaaatgtg aagtactacg caaagatcgt cagtgagaaa gcagttcttc
     1021 gcagacttat caaggcaaat gaagagattg ccaacacctg ctatctggag aaggagaata
     1081 cagaaattat tctggaagaa gcggaaaaaa aactgttcaa tattctccag agacgcaaca
     1141 atgaagaata tgttccaatc cagcaggtag ttctgaatgc cattaataac attgagaaag
     1201 cgtctaagct taagggttct gtaacaggta ttcccacagg ctttatggat ctggattata
     1261 aaacatccgg tatgcatcct tctgatctgg tgctgatcgc agcccgtcct tccatgggga
     1321 aaacagcctt tgtactgaac attgcacagt atatggcatt ccgaaaggat gtcacagttg
     1381 ccatattcag tctggaaatg tcaaaggaac agctggtgaa ccgacttctg gcaatggaat
     1441 ctcatgtaga ttcccagaat atgagaacag gtaatctgaa agacgaggac tggacaaaac
     1501 tggtagaagg agcagatatt atcggtaaat ccaatctgat cattgatgac acgccgggta
     1561 tttccattgc ggaaatgcgc tccaaatgca ggaaatataa gctggaacat aatcttggga
     1621 ttatcatgat cgactatctg cagctgatga gcggaagtgg aaaaagtgat tccagacagc
     1681 aggaaatttc agatatttcc cgttctctga aagctcttgc cagagagctg aatgtgccgg
     1741 tgatcgcatt gtctcagctc agccgtgcgg tggaacagcg tccggatcac agaccaatgc
     1801 tttcggatct tcgagaatcc ggagccattg aacaggatgc cgatgtggta atgtttatct
     1861 acagagatga ctattatcat aaggatacag agaagaagga cattgcagag atcatcattg
     1921 ccaaacagag aaacggtccc atcggaacag tagaactggt atggctgccg agatatacac
     1981 agtttgtgaa tatgaagaaa taatctatct cagaggagtt tgatctttga cttgaataat
     2041 attatagcaa tcctctaaaa taatgcatct taatccagcc catcagagtg tgaaaacctg
     2101 ctgaattgtc ttaaatgcat tatttacgag gattgctata gaaaaatttt acagggaaac
     2161 agtatcaggg ggagaatccg gtactgtttc cctgtttttt gtgtcaaaat aaagttttat
     2221 agggaagttt gtaaatcgaa act
//
LOCUS       DBMDXG010000368         2239 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10181_length_2239_cov_1.180543, whole genome shotgun sequence.
ACCESSION   DBMDXG010000368 DBMDXG010000000
VERSION     DBMDXG010000368.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10181_length_2239_cov_1.180543"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="ACZPD3_09180"
     CDS             <1..1489
                     /locus_tag="ACZPD3_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1416195.1"
                     /translation="GMRNEVLRMERVTYKEDEVVKLEDFNLQIFQGEIMGFMPVNSHG
                     KTALLKLLERNLPLYDGYIYYGGEKVNTWKENYKAANRISIIQEKSSLAGNLNIADNI
                     FVLRPGFRQWVIRTKILNRQMEPFLEDIGMDIPLNKDVEKLSTFERIIVELLRAVIMG
                     YHLIVLDEVGALVSDDELKKLHGIIRRYVKKGFSFLYICSHLEEISSICDRCALFTNG
                     RIQKILEREELFNDPPVTYMTEYNDMVRYHTEKREGQQAAPVVFQWKGYGEDAACNFS
                     FDVHKGECLAVQIMDARGMEELRKILTGDILPESGEILLNGKQTEIPGNGRIAVIQER
                     TTKTMIFPKLDYMNNLCICLAEKVPSIWHNKRLQKSIRNEYSPILGTDVFEMPVEELS
                     EKQKYQLVYTRVFLQKPEILFCIQPFCGADLAHRMFIWSMLEKFLDKGISVVILSLNL
                     SDSLAMADRLLILGENGKKKEIVRENFHKITSSVPWLHRYPSERK"
     gene            complement(1614..>2239)
                     /locus_tag="ACZPD3_09185"
     CDS             complement(1614..>2239)
                     /locus_tag="ACZPD3_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR1416196.1"
                     /translation="NPTLVQQVGQAGAEEVKENNLAVWLTPAVNIHRNPLCGRNFEYY
                     SEDPLIAGKMGAAMVKGIQSQHIAASVKHFAANNKETNRKHSDSRVSERALREIYLKG
                     FEIIVKEAQPWTIMSSYNAINGHRASENRELLEDVLRGEWGFKGMVTTDWWTRAEHYK
                     EIKAGNDVKMGTGFPERVKKAMEMGQLGRPELEHCARRVLELILKID"
BASE COUNT          616 a          444 c          552 g          627 t
ORIGIN      
        1 tggtatgaga aacgaagttc tcagaatgga gcgtgtgacg tataaagagg atgaggttgt
       61 gaagctggag gacttcaatc ttcagatttt tcagggagag attatgggat ttatgcctgt
      121 taacagtcat ggaaaaacag ctcttctgaa gcttctggaa cggaatcttc ccctctatga
      181 cggttatatt tattatggag gggaaaaggt aaacacctgg aaagagaatt acaaggcagc
      241 aaacaggatc agcattatcc aggagaaaag ttctctggca ggaaatctga acattgcaga
      301 taatattttt gtcctgcgtc cgggctttcg ccagtgggtg atccgcacaa agatcctgaa
      361 ccggcagatg gaaccttttt tagaagatat cggaatggac attcccctta ataaagatgt
      421 ggagaagctt tctacttttg aacggatcat tgtagaattg ctccgggctg tgattatggg
      481 atatcatctg atcgtgctgg acgaagttgg agcccttgtc agtgatgatg agttgaaaaa
      541 gctccatgga attatccgtc gttatgtgaa aaaagggttt tcttttcttt atatctgttc
      601 tcatctggaa gagatttcca gtatctgtga cagatgtgcg ctgtttacca atggaagaat
      661 ccagaagatt ctggaaagag aggagctgtt caatgatccg cccgtaactt acatgacgga
      721 atataacgac atggtccgtt atcatacgga aaaaagggaa gggcagcagg ctgcaccggt
      781 tgtttttcaa tggaagggct acggagagga tgcagcctgc aatttttcct ttgatgtgca
      841 taaaggagaa tgcctggccg tacagattat ggatgccagg ggaatggagg agctacggaa
      901 gattctgacc ggagatattt taccggaaag tggagaaatc ctgttgaatg ggaagcagac
      961 agagattccg ggaaatggca ggattgcagt aatccaggaa cgtactacaa aaaccatgat
     1021 ttttccgaaa ctggattata tgaataatct ctgtatttgt ctggcagaaa aagttccttc
     1081 aatctggcac aacaaacgtc ttcagaaaag tattcggaat gaatacagtc ccatattagg
     1141 gacagatgtt tttgaaatgc cggtggagga actgagtgaa aagcagaagt atcagctggt
     1201 gtatacaaga gtatttttgc agaaaccgga gattctgttt tgtattcagc ctttctgcgg
     1261 cgctgatctt gctcacagga tgtttatctg gagtatgttg gaaaagttcc tggataaggg
     1321 gatttcggtg gtgatcttat ctttgaattt atcggattcc ctggctatgg cagatcgcct
     1381 gttgattctt ggggaaaatg ggaaaaagaa ggaaattgta agagaaaatt tccataagat
     1441 tacctcgtcg gtgccctggc tgcataggta tccgtcagag agaaaataaa atgtataaaa
     1501 cataatacta ataattcgga ttgtattttc aaacacgctc cagagcctaa ataagaagcc
     1561 catggcaaca gaaataaaat tgccatgggt ttctttataa ctaaaattca atttcagtca
     1621 atcttcagaa tcagttccag cacccgtctt gcacagtgct ctaattccgg gcggccaagc
     1681 tgtcccattt ccatggcctt tttcacacgc tcggggaaac cggttcccat ttttacgtca
     1741 tttccggcct tgatttcttt gtaatgttca gctcttgtcc accagtcagt tgtaaccatg
     1801 cccttgaagc cccactcgcc gcggagaaca tcttccagaa gctcgcggtt ctcagaagcg
     1861 cggtgcccat tgattgcatt gtaggaggac atgatggtcc atggctgtgc ctcttttaca
     1921 atgatctcga aacctttcag atagatctcg cgaagtgcac gctcggaaac acgggaatcg
     1981 ctgtgctttc tgttggtctc tttgttattt gctgcgaaat gctttacaga agctgcaatg
     2041 tgctgggact ggataccttt taccatagct gcgcccatct ttccggcaat cagcggatcc
     2101 tcggaatagt actcgaagtt tcttccgcaa agtgggtttc tgtggatatt cacagcaggg
     2161 gtaagccata ctgcaagatt gttctccttc acttcctcgg caccagcctg tcccacctgc
     2221 tgaacaagtg tcgggttcc
//
LOCUS       DBMDXG010000369         2233 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10220_length_2233_cov_1.612570, whole genome shotgun sequence.
ACCESSION   DBMDXG010000369 DBMDXG010000000
VERSION     DBMDXG010000369.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_2233_cov_1.612570"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="ACZPD3_09190"
     CDS             <1..1274
                     /locus_tag="ACZPD3_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR1416197.1"
                     /translation="QLLTDTIAEDNTLSEDERKNLSGECDLIMSFLMYNDITGMSVLH
                     RQASSKMTRPAISIRKTGSWTFGSPSVLMMFHRRSGTLDAELTAMNECMPHYYRITQG
                     HGQGAELLMNAEAAFMQGNFSDAQILLEQTYSTIASNGQHNISLCCDFLAARLSLFQE
                     GVTFVKIPEVKRKELLSLHNMMWLNIFDSTYAYYYALIRMPEKIPALFKDHMLSTVSF
                     LSPCRPMMEMIENQVFLSQKMYAKVIGRSETLLPFCEKMHYELVSLHVQIQTASAYAM
                     LGKHHDARQLLQKALGHAMPDGFLIPFVENYTYIKDVLGSINSIASEPFTDRILSLGS
                     VYEQHCLRLSSRNSRPEILNMLNSREAEIAALITDRLSNREIAERLFLSEGTVKQYIN
                     QIYSKLMINGDTRTKRKQLAELISSINKGLT"
     gene            complement(1354..1569)
                     /locus_tag="ACZPD3_09195"
     CDS             complement(1354..1569)
                     /locus_tag="ACZPD3_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416198.1"
                     /translation="MRAAIKEAGIDDIGLCTDEKIHTTLAMVHTYPDGDRDFSFYRNP
                     GADMMLNKTEIPEDILKETEMQISKKL"
     gene            complement(1798..>2233)
                     /locus_tag="ACZPD3_09200"
     CDS             complement(1798..>2233)
                     /locus_tag="ACZPD3_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HIR1416199.1"
                     /translation="EYNNVVIKDTRLTLDQIEGRTVDLELVIRPADKDNLYKKFELCF
                     AENEKYHSTLCFRPDESVLKIDRKFSGSERALVHQSSCLVNGDSNELKLRVILDKFSV
                     EVFINDGEQVMSAVILTKQEAKGISFFADGAAKLDIVKYDLL"
BASE COUNT          638 a          519 c          414 g          662 t
ORIGIN      
        1 agcagcttct gacagatacc atagctgaag ataacacttt gtccgaagat gagagaaaga
       61 atctctccgg agaatgtgat cttattatga gttttctgat gtataacgat atcaccggta
      121 tgagcgttct gcaccgtcag gccagttcta aaatgaccag acctgccatc agtatccgca
      181 aaacaggaag ctggaccttc ggttctcctt ctgttctgat gatgtttcac cgcagatccg
      241 gaaccctgga tgcagaactg acagctatga atgaatgtat gccccactat tacaggatta
      301 cccagggaca tggacagggt gcggaattgc tcatgaatgc agaggcagca tttatgcagg
      361 ggaatttttc agatgcacag attctgctgg agcagactta ttccaccatt gcctcaaacg
      421 gacagcacaa tatttcgctc tgctgtgatt tccttgctgc aaggctttcc ctttttcagg
      481 agggtgtcac ctttgtgaag atccctgaag taaagcgtaa ggagcttctg tctctccaca
      541 atatgatgtg gctgaatatt tttgacagta cttatgctta ctactatgca ttaattagaa
      601 tgccggaaaa gattcctgca ttatttaaag accatatgtt atccactgtc agctttcttt
      661 ctccctgcag acctatgatg gaaatgatcg agaatcaggt ctttctttca cagaaaatgt
      721 acgcaaaagt tataggcaga agcgaaacgc ttctgccctt ctgtgagaaa atgcactatg
      781 aacttgtgtc tctccacgtt cagattcaga ctgcttctgc ttacgcaatg ctgggcaaac
      841 atcatgatgc acgacagctt ctgcagaaag ccttaggaca cgccatgccg gatggctttc
      901 tgattccttt tgtagaaaat tatacctata tcaaggacgt gctgggcagt attaattcca
      961 tagcatcaga gcctttcaca gaccggatcc tttcccttgg ttctgtttat gaacaacact
     1021 gcctccgcct gtccagccgt aattccagac ctgagatcct aaatatgctg aacagcagag
     1081 aagcagaaat cgcagcttta atcaccgacc gtctgagcaa ccgcgaaatt gcagaaaggt
     1141 tatttctctc tgaaggcact gtgaagcaat atatcaacca gatttattcc aagttgatga
     1201 tcaacggtga tacacgcacc aaaaggaaac aactggcaga gctgatttct tccataaaca
     1261 agggccttac ctgacacttt gagaaaaaaa agcaaggaaa ttcacatgac caaagtcttg
     1321 cgatttcctt gctatttggc aagaattaca aatttaaagt tttttactta tttgcatttc
     1381 tgtttccttg agaatatcct cgggaatctc agttttattc agcatcatgt ctgctcccgg
     1441 attacgatag aaggaaaaat ccctatctcc atccggatat gtatgtacca tagcaagcgt
     1501 agtatgaatt ttttcatctg tacataagcc aatgtcatca attcctgctt cttttatggc
     1561 tgctctcaac tgatctccaa aaaacattac caaccttacc aataaatgct acattatgtc
     1621 ccaatttata gcaatcctct gaaataatgc atcttaatcc agcccatcag agtgtgaaat
     1681 cctgcgtcag atatctttgc cgtgcgtcag cacgcttgca gatatcttcc ttgtctttcg
     1741 cactctactg aactgtctta aatgcattat ttacgaggat tgctatagaa cgctttatta
     1801 taataaatca tatttcacta tatcaagctt agctgcacca tcagcaaaga aagaaatccc
     1861 ctttgcttcc tgctttgtaa gaataaccgc agacataacc tgctcgccgt cattaataaa
     1921 tacttccaca ctaaacttat ccaaaatcac acgcagtttt agttcattgc tgtcaccgtt
     1981 taccagacaa ctgctctgat gaaccagcgc ccgttcgctt cctgagaatt ttctatcgat
     2041 ttttaaaaca gattcatccg gacggaagca taatgtagaa tgatatttct cattctctgc
     2101 aaaacagagt tcaaattttt tataaagatt gtctttatct gccggtcgta tcacaagttc
     2161 cagatctacc gttcgtcctt caatctgatc cagtgtgagc ctggtatctt ttatcacaac
     2221 attgttatac tcg
//
LOCUS       DBMDXG010000370         2231 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10228_length_2231_cov_2.153521, whole genome shotgun sequence.
ACCESSION   DBMDXG010000370 DBMDXG010000000
VERSION     DBMDXG010000370.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10228_length_2231_cov_2.153521"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..700
                     /gene="dapB"
                     /locus_tag="ACZPD3_09205"
     CDS             29..700
                     /gene="dapB"
                     /locus_tag="ACZPD3_09205"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR1416200.1"
                     /translation="MVSDVADQGKNSYPVYTNMEECQVEADALIDFSSAKATDALLAY
                     CEKRKLPVVLCTTGLSEEQLAKVEETSKNVAVLKSANMSLGINTLMKLLQDAAKVLAT
                     AGFDMEIVEKHHRLKLDAPSGTALALADSINEAMDNQYHYIYDRSQRREMRDDKEIGI
                     SAVRGGTIVGEHEVIFAGPDEVIEFKHTAYSKAVFGKGAVEAAKFLAGKPAGRYDMSD
                     VISAK"
     gene            complement(849..2108)
                     /locus_tag="ACZPD3_09210"
     CDS             complement(849..2108)
                     /locus_tag="ACZPD3_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HIR1416201.1"
                     /translation="MKRKRNHSLRSSVFIFLAALFLLFTCSVSTIYASTLQKPDIAAS
                     GKFVKDGDYWIYRYDDKTIAKNVFLKIDKKTYYFNKLGHRWCSWHTIKGKNYYFGTRS
                     QGYLIKNSLIKYKGNYYYVGKDGAMVTGWYTDKSGKKYYFGKDGKAVTGKHKIKGTYY
                     YFNQNGTVTHTGLNYSLSSDCALLMNADTGQIIYGKNENVAHANASTTKIMTCILALE
                     NCKLNEKVKFSPYAASIEPSKLYANAGEIFYLKDLLYSLMLPSHNDTAVAIAEHVSGS
                     TAKFVKLMNKKAAEIGCTNTHFATPNGLDAGYNHYTTASDLAKIAQYAIKKPMFRKLV
                     STGYYSFSNLNTGRSYSIGTTNALLGNVPGVQGMKTGYTNKAGYCFVGLSYSARGNTY
                     ISVVLGADSSSARWQDSRILLTYAYNH"
BASE COUNT          654 a          399 c          519 g          659 t
ORIGIN      
        1 tcctaagatg tagggaaggt aataggattt ggtatcagat gtggcagatc agggaaagaa
       61 cagctaccct gtatatacaa atatggaaga atgtcaggta gaggcagatg cccttatcga
      121 cttctcttct gcaaaagcaa cagatgcact tcttgcttac tgtgagaaac gcaaactccc
      181 ggttgtatta tgtacaaccg gtttaagcga ggagcagctc gcaaaggtgg aagagacatc
      241 taagaatgtc gcagttctga aatctgcaaa tatgtctctt ggaatcaata ctctgatgaa
      301 actgcttcag gatgcggcaa aggttctggc aacagcagga tttgacatgg agattgttga
      361 gaagcatcac agactgaaac tggatgcacc aagcggaaca gcacttgcac tggctgacag
      421 cattaatgag gcaatggata accagtatca ttatatctat gaccgcagcc agagacgtga
      481 gatgcgggat gataaggaaa taggtatctc agcagttcgc ggtggaacaa tcgtaggaga
      541 acacgaagtg atcttcgcag gtccggatga agtgattgaa tttaagcata ctgcatattc
      601 caaagctgta tttggaaagg gtgctgttga agctgcgaaa ttccttgcag gcaaaccggc
      661 aggaagatat gatatgagcg atgttatcag tgcaaaataa tcaggaatgc agtaaaaatt
      721 gataactggt aattttcagt atcttcatag cagtgaaata aaatatataa aaggaaagtg
      781 tatctgatgt ttcagaacac tttccttttt ttgtaatatt ggcctgtatg ctgctataat
      841 aattctgatc aatgattata tgcgtaagtc agcaatattc tggaatcctg ccagcgggca
      901 gaagatgaat ctgcacccag gacaacagaa atataggtgt ttccccttgc ggaataagaa
      961 agtccgacaa aacaatagcc tgctttgttg gtatagcctg ttttcatacc ctgaactccc
     1021 ggaacattgc cgagcagggc gttggtggtg ccgatagaat agcttctgcc ggtgttgaga
     1081 ttagagaaag aataatagcc ggtgcttacc agtttgcgga acataggctt tttgatcgca
     1141 tactgggcaa tttttgccag gtcagaagct gttgtgtaat ggttataccc tgcatccaga
     1201 ccatttgggg ttgcgaaatg tgtattcgta catccgatct ctgctgcttt tttattcatt
     1261 aatttaacaa actttgcagt ggaaccgctg acgtgttcgg caatggcaac tgcggtgtcg
     1321 ttgtgggagg gaagcatcag ggaataaagc agatctttca gataaaatat ttctcctgca
     1381 tttgcataaa gcttgctggg ttcaatagat gctgcgtaag gggagaactt tactttttcg
     1441 tttaatttgc agttttcaag tgcaaggata caggtcataa tcttggttgt gctggcgttg
     1501 gcatgagcaa cattttcatt tttgccataa ataatctgac cggtatctgc attcatgaga
     1561 agtgcacagt cagaggaaag ggaataattt aatccggtgt gggtgacagt accattctgg
     1621 ttaaagtaat agtaagttcc cttgatcttg tgttttcctg taactgcttt tccgtctttg
     1681 ccgaaatagt atttctttcc ggatttgtca gtgtaccatc cggtaaccat agcaccgtct
     1741 ttaccgacat agtaataatt tcctttatat ttgataagag aattctttat cagatatccc
     1801 tgagatctgg ttccgaaata ataatttttg cctttgatcg tatgccagct gcaccagcgg
     1861 tgtccgagtt tgttaaaata ataggtcttt ttgtcaattt tcagaaatac atttttggcg
     1921 atggttttat catcatagcg gtaaatccag taatcgccgt cttttacaaa ttttcccgat
     1981 gctgcgatat caggtttctg aagtgtggat gcataaatag tagaaacact gcaggtaaac
     2041 agcaggaaaa gtgcagccag aaatatgaaa acagagctgc gaagtgaatg gttccttttt
     2101 cttttcatga taacctcctg taaatatgag ttatagatat tccgtaaaat actgcagtca
     2161 atccatctca ttaatgagtg aaatcctgct atagcaatgt gttagtgtga cgacaagatt
     2221 gtttgagaaa t
//
LOCUS       DBMDXG010000371         2218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10300_length_2218_cov_1.701464, whole genome shotgun sequence.
ACCESSION   DBMDXG010000371 DBMDXG010000000
VERSION     DBMDXG010000371.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10300_length_2218_cov_1.701464"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="ACZPD3_09215"
     CDS             <1..930
                     /locus_tag="ACZPD3_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HIR1416202.1"
                     /translation="HPARENHRIIPITVNDSPWGFQYSPYVYYNEHCIVFNSQHVPMK
                     IEKNTFIKLFDFVKLFPHYFLGSNADLPIVGGSILSHDHFQGGHYTFAMAKAPIEKHV
                     TIPGYEDVEAGIVKWPLSVLRIRHKDEKRLIELATHVLEAWRGYTDESAFIFAETDGE
                     PHNTITPIARRSGDMFELDLTLRNNITTDEHPLGVYHPHAEYHHIKKENIGLIEVMGL
                     AVLPARLKEELELLAEYILEGKDISSNENIEKHAAWVAEFLPKYDNLDKDNIQDVLRK
                     EVGNVFVHVLEDAGVYKCTAEGREAFMRFINKL"
     gene            1124..>2218
                     /locus_tag="ACZPD3_09220"
     CDS             1124..>2218
                     /locus_tag="ACZPD3_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR1416203.1"
                     /translation="MYRKRHKRGKRLASVFLTVALITATVPVQAAEFGTPDSFEEIFS
                     SGEEDSCITDTENTLPAASDSEKVMASELSPTPTVTSTPEGTPALTETPTPSVTPMPS
                     STPTPSVTPSPSPTPTINPEKEVMLRFIDGEGNECEQLRTVMSWGESLILPNVPDTGA
                     PDMWKLEKNEKLGDAITLKGGDILTLKKGESWNLFLEKGILNFYMPKKCTVSLYNNSG
                     TSVFSNGILQAYETKNVILPDMPSSKYINYGWTDTKGSSAVKYELNSEFTVTGDTDFY
                     IVRRTALQVNFKTNTGASNSKFTRLSQKVGKGLTVTMPQVPVKTGYQSLGWSKNKKAS
                     KADYKAGQNVTVSKTLTLYAVYKKLPYTVTF"
BASE COUNT          708 a          432 c          502 g          576 t
ORIGIN      
        1 catcctgcca gagagaatca cagaatcatt cctatcactg taaatgacag tccctgggga
       61 tttcagtatt ccccatatgt gtattacaat gagcattgca tcgtctttaa cagtcagcat
      121 gttccgatga agattgagaa gaatacattt atcaaacttt ttgattttgt aaaacttttc
      181 ccgcattact tcctgggatc caatgcagac ctgcctatcg taggcggttc tattttaagt
      241 cacgatcatt tccagggcgg acattatact ttcgcaatgg caaaagcacc gatcgagaaa
      301 catgtgacta ttccgggata tgaggatgta gaagcaggta ttgttaaatg gcctctttca
      361 gttttaagga tccgtcataa ggatgagaag agactgatag agcttgctac acatgttctg
      421 gaagcgtgga gaggttatac agacgaaagt gcgtttatct ttgctgaaac tgatggagaa
      481 ccgcacaata cgatcacacc gatcgcccgc agatccggag atatgtttga actggatctc
      541 acacttagaa ataacattac tactgatgag catcctcttg gtgtatacca tccgcatgca
      601 gagtatcacc atatcaagaa agagaatatt ggtctgatcg aagtaatggg acttgccgta
      661 cttccggcaa gattaaaaga agaactggaa cttctggcag aatatattct ggagggaaaa
      721 gacatttctt ccaatgagaa tattgagaaa catgcagcgt gggttgcaga attccttccg
      781 aaatatgaca atcttgataa agacaacatt caggatgttc tcagaaaaga agtcggtaat
      841 gtattcgttc atgtacttga ggatgccggc gtatataaat gtactgctga aggcagagaa
      901 gcatttatga ggtttatcaa taaattataa ggaatatttt caaccataag aaaacagtgt
      961 ttacatagca ggatgacaaa tctggtatta tttacatatg agcaggttgt ggcggttctg
     1021 atcaaaagcc gggcagtctg aagatgtttt tgaatgattg ggaatgcata gagaacgtcc
     1081 cgcatatttt atgttgggac gtttttgcaa aatggaggta aatatgtaca gaaaaagaca
     1141 caaaagagga aagcgtctgg caagtgtgtt tctgactgtg gcactgatca ctgcaacagt
     1201 tcccgtacag gcagcagagt tcggcactcc ggacagtttt gaagagattt tcagttcagg
     1261 ggaagaagat tcgtgcataa ctgatacaga aaatactttg ccggcagctt ctgattcgga
     1321 gaaagtaatg gcatcggaac tgtcaccaac accaacagtg acttcgacac cggagggtac
     1381 acctgcactg acagaaacac cgactccttc ggtcacaccg atgccgtctt caacaccgac
     1441 tccttcagtc acaccatcac catctcccac gccgacgatt aatccggaga aagaggtaat
     1501 gctccggttt atagacggag aaggaaatga atgtgaacag ctgcgaacag tgatgtcctg
     1561 gggtgaatct ttgatccttc ccaatgtacc ggatacaggt gcaccggata tgtggaaact
     1621 ggaaaaaaat gaaaaacttg gagatgcgat cactctgaag ggtggagata ttcttactct
     1681 gaagaaaggc gagagctgga atctgtttct cgaaaaaggc atcctgaact tctatatgcc
     1741 aaagaaatgt acggtttccc tttataataa ttcaggaacc agtgtgtttt ccaatggaat
     1801 tctgcaggca tatgagacaa aaaacgtcat acttccggat atgccatcat ctaaatacat
     1861 taattatgga tggacagata caaagggaag ttctgctgta aaatatgaac tgaattcaga
     1921 atttacagtg accggtgaca cagattttta cattgtgcgc cgtacagcac ttcaggtgaa
     1981 ttttaagacc aatacagggg caagcaacag taaatttacc agactgagtc agaaagtcgg
     2041 aaaaggactt acagttacaa tgccgcaggt acctgtcaag acaggatatc agtctctggg
     2101 atggtcaaag aataaaaaag cctccaaagc tgactataaa gcaggccaga atgtgactgt
     2161 atcaaaaaca ctgactctct atgctgtata taagaaattg ccttacacag ttactttc
//
LOCUS       DBMDXG010000372         2211 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10328_length_2211_cov_1.913744, whole genome shotgun sequence.
ACCESSION   DBMDXG010000372 DBMDXG010000000
VERSION     DBMDXG010000372.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10328_length_2211_cov_1.913744"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="ACZPD3_09225"
     CDS             <1..472
                     /locus_tag="ACZPD3_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR1416204.1"
                     /translation="ETIISTVDSVKTADFKMSFDPTLVRGMSYYTGPIFEISMDEFGG
                     SVGGGGRYDEMIGKFTGNNTSACGFSIGFERIVMLLLERNYEIPTKNGKKAYLIEKNM
                     PADKMLTILKQAQEERNAGTQINISIMKKNKKFQKDQMTAEGYTEFVEFFKDRM"
     gene            569..>2211
                     /gene="aspS"
                     /locus_tag="ACZPD3_09230"
     CDS             569..>2211
                     /gene="aspS"
                     /locus_tag="ACZPD3_09230"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR1416205.1"
                     /translation="MAESMQGLHRTCRCAEVTKEMIGKEVVLMGWVQKARDKGGIIFV
                     DLRDRSGIMQLIFENGSIDEEGFAKAGKLRSEFVIAVTGVVEERGGAVNKNLATGEIE
                     LRVKSLRVLSEAEVPPFPIEENSKTKDEIRLKYRYLDLRRPDLQKNIMLKSKVMMITR
                     QFFAKEGFLEIETPMLGKSTPEGARDYLVPSRVHPGHFYGLPQSPQLYKQLLMCSGYD
                     RYIQIARCFRDEDLRADRQPEFTQIDMELSFVDVDDVIDVNERFLAYLFKEVLDIDVK
                     LPIQRITWQEAMDRFGSDKPDMRFGMELHDVSDVVKNCGFSVFTSALENGGSVRGINA
                     EGQGAMPRKKIDKLVEFAKGYGAKGLAYIAIAEDGTRKSSFAKFMTDEEMDALVAAMD
                     GKAGDLLLFAADKKKLVYDVLGALRLELAKQMDLLDKNEYRFVWVTEFPLLEWSEEEN
                     RFTAMHHPFTMLMEEDLPLLDTDPGKVRAKAYDIVLNGNEIGGGSVRIHQDDIQERMF
                     EALGFTKEAAHEQFGFLLDAFKYGVPPHAGLAYGLDRLV"
BASE COUNT          689 a          379 c          548 g          595 t
ORIGIN      
        1 tgaaacaatc atttctactg tagattctgt taagacagca gattttaaga tgtctttcga
       61 tccgactttg gtacgtggaa tgtcttatta tacaggaccg atttttgaaa tctctatgga
      121 tgagtttggc ggcagcgtag gcggcggtgg acgctatgat gagatgatcg gtaaatttac
      181 aggaaacaat acatctgcct gcggattttc aattggattc gagagaatcg taatgcttct
      241 tcttgagaga aattatgaaa tcccgacaaa gaatggtaag aaagcatatc ttatcgagaa
      301 gaatatgccg gcagacaaga tgctgacgat tctgaaacag gctcaggaag agcgtaatgc
      361 cggaacacag atcaatattt ctattatgaa gaagaataag aaattccaga aagatcagat
      421 gacagcagag ggctatacag agtttgtaga attctttaaa gacagaatgt aataattcgt
      481 aactgttcag caacattgcg agatagcaga ctgccggata gttacaacaa ttcttataat
      541 atagaaagca tgaaaagagg aaagaaacat ggctgaaagt atgcagggac tgcacagaac
      601 atgccgatgt gcagaagtaa caaaggaaat gatcggcaaa gaagttgtcc ttatgggatg
      661 ggttcagaaa gcccgcgata aaggcggaat tattttcgta gatttaagag accgttccgg
      721 tatcatgcag ctgatttttg agaatggcag cattgatgaa gaaggttttg caaaagcagg
      781 taagctgaga agtgaattcg taatcgctgt taccggtgtg gtagaagaaa gaggcggcgc
      841 tgttaacaag aatcttgcaa caggtgaaat tgagcttcgc gtaaaatccc ttcgtgttct
      901 gtctgaagca gaggtacctc cgttcccaat tgaagagaac agcaagacaa aagatgaaat
      961 ccgtctgaaa tatcgttatc ttgacttaag aagacctgac cttcagaaga atattatgct
     1021 taagagcaaa gtcatgatga ttacaagaca gttcttcgca aaagaaggat tccttgaaat
     1081 tgaaactccg atgcttggta agagtacacc ggaaggtgca agagattatc tggtaccgtc
     1141 tcgtgttcat ccgggacatt tctatggtct gccacagtct ccgcagctgt acaaacagct
     1201 tctgatgtgc tctggttatg accgttatat ccagattgca agatgtttcc gtgacgaaga
     1261 ccttcgtgcg gacagacagc cggaatttac ccagattgat atggaactct cttttgtaga
     1321 tgttgatgac gtaattgatg taaatgagag attccttgct tatctgttca aggaagtact
     1381 tgatattgat gtaaaacttc caatccagcg tatcacatgg caggaagcta tggacagatt
     1441 tggttctgat aaaccggata tgcgtttcgg aatggaactt catgatgtaa gtgatgtagt
     1501 taaaaactgt ggattcagcg tgtttacttc tgcgttggaa aatggcggca gtgttcgtgg
     1561 tatcaatgca gaaggacagg gcgctatgcc acgtaagaaa attgacaaac tggttgagtt
     1621 tgccaaaggt tatggtgcaa aaggccttgc atacattgca atcgctgaag atggcacaag
     1681 aaaatcttct tttgctaaat tcatgacaga tgaagagatg gatgctctgg tagcagctat
     1741 ggatggtaaa gcaggagact tacttctgtt tgcagcagat aagaagaaac tggtttatga
     1801 tgtacttggc gcacttcgtc tggaacttgc aaagcagatg gatcttcttg ataagaatga
     1861 gtatcgtttc gtatgggtaa cagagttccc gcttcttgaa tggagtgaag aagagaatcg
     1921 tttcacagca atgcatcatc catttaccat gcttatggaa gaggaccttc cacttctgga
     1981 tacagatcct ggaaaagttc gtgcaaaagc atatgatatc gtattaaacg gtaatgaaat
     2041 tggtggagga agtgtcagaa tccatcagga tgatattcag gagagaatgt ttgaagcact
     2101 tggattcaca aaagaagctg ctcacgagca gtttggattc cttctggacg cattcaaata
     2161 tggagttccg cctcatgccg gactggcata cggacttgac cgtctggtaa t
//
LOCUS       DBMDXG010000373         2209 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10339_length_2209_cov_1.733397, whole genome shotgun sequence.
ACCESSION   DBMDXG010000373 DBMDXG010000000
VERSION     DBMDXG010000373.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10339_length_2209_cov_1.733397"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            144..758
                     /gene="yedF"
                     /locus_tag="ACZPD3_09235"
     CDS             144..758
                     /gene="yedF"
                     /locus_tag="ACZPD3_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HIR1416206.1"
                     /translation="MKHEVNAMGKQCPIPVVMTKKVIDNAAVGDEIEILIDNETAVNN
                     LSRLANKTGCTFVSEKLGDKKYQVKMAVKTEQTGGTLEEEEFVCEAPHKKVTVAVISS
                     NVMGNGDDELGKILIKGFIYALSQMETHPDTILFYNGGAKLTTEDSESLEDLKKMEEE
                     GVEILTCGTCLKHYGLMEKLMVGKVTDMYTIAERMTGADKVIRP"
     gene            complement(816..2027)
                     /locus_tag="ACZPD3_09240"
     CDS             complement(816..2027)
                     /locus_tag="ACZPD3_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR1416207.1"
                     /translation="MKKNFFLHSLPFRLLVGVAFGICIGLLLNIIDESAITTAVLNVV
                     VTSKYILGQLISFSVPLIIIGFIAPSITRLGNNASRMLGVAVSIAYASSLGAALFSAL
                     SGYLLIPHLSFSSTASHLKALPDVVFELSIPQIMPVMSALVLAIMLGLASAWTKASLI
                     SSFLEEFQKIILAIVSRVIIPILPYFIGLTFCSLAYEGTITRQLPVFLQVIVIVLIGH
                     FIWIALLYILAGIYSRENPLKVVKHYGPAYLTAIGTMSSAATLPVALQCAHRAKPLRK
                     DLVDFGIPLFANIHLCGSVLTEVFFCMAISKILYGAVPAPGAMVLFCVLLGIFAIGAP
                     GVPGGTVMASLGIITGILKFGDSATALMLTIFALQDSFGTACNVTGDGALTLILTGYA
                     RYHNIREQKLQ"
BASE COUNT          762 a          383 c          531 g          533 t
ORIGIN      
        1 gcaacctact tgggatttat ttgtatcgcc tcaatattaa tttggttaaa ataaacaatt
       61 taaaatgttt ttcagacacg ctctagggag attgcgaaga gtagtaagca gtgaatataa
      121 agaataacaa gaggtgacag aaaatgaaac atgaagtaaa tgcaatggga aaacagtgcc
      181 cgattccggt agtgatgaca aagaaggtta ttgataatgc ggcagtcggt gatgagattg
      241 aaattctgat cgataatgaa accgcagtga ataatctgag cagacttgca aataaaacag
      301 gctgtacgtt tgtatctgaa aaactgggag acaaaaagta tcaggtgaag atggcagtaa
      361 agactgaaca gacaggtggt accttggaag aagaggaatt tgtctgcgaa gcaccgcata
      421 aaaaagtgac ggtagcggta atctcctcaa atgttatggg aaatggtgat gatgaactgg
      481 gaaagatttt gatcaaagga tttatctatg ccctttctca gatggaaaca catccggata
      541 caatcctttt ctataatgga ggtgcaaaac ttacaacaga agattctgaa agtctggaag
      601 atctgaaaaa gatggaagaa gagggcgtgg aaatcctgac ctgcggtacc tgcctgaagc
      661 attatggact gatggagaaa ctgatggttg gaaaagttac cgatatgtat acgattgcag
      721 agcgtatgac aggtgcagat aaggtgattc gtccgtaaga aaatgaaggc catcatatct
      781 gagaaaaaag acagatatga tggtattttg aacttttatt gcaatttctg ttctctgata
      841 ttgtggtatc tggcatatcc ggtaagaatc agagtcaggg caccatcgcc tgttacgtta
      901 caggctgtcc caaaactgtc ctgaagggca aagatagtca gcatcagagc tgtagcagaa
      961 tcgccgaatt tcaggatacc ggtgataata ccgagagatg ccataacagt gcctcccggt
     1021 acacccggag caccaatggc aaaaattcca agcaggacac agaaaagaac catggccccc
     1081 ggcgcaggaa ctgcaccata aagaattttg gagattgcca tacagaagaa tacttctgtg
     1141 aggacagaac cacagagatg aatattggca aacagcggaa taccaaaatc taccagatcc
     1201 tttcggagag gttttgccct gtgtgcgcat tggagggcga ccggcagtgt ggcagcagat
     1261 gacatggtac ctatggctgt gaggtaggcg ggaccataat gtttcacaac tttcagagga
     1321 ttttctctgg aataaattcc tgcaagtata tatagaagag ctatccagat aaaatgtccg
     1381 atcagaacaa tgacgattac ctgaaggaat acaggaagct gtctggtgat tgttccttcg
     1441 tatgccagtg aacagaaagt cagtccgatg aagtatggga gaatcggaat aatgactctt
     1501 gatacaatag ccagtatgat cttctgaaat tcttcaagaa aagaagaaat cagactggct
     1561 tttgtccatg cagatgcaag tcccagcata atggccagta caagtgcact catgacaggc
     1621 attatctgtg gaatggaaag ttcaaaaact acatccggca aagctttgag atgactggca
     1681 gtggagctga aggacaaatg tgggatcagc aggtatccgg ataatgcaga gaataaagca
     1741 gctcccagtg aggaagcata agcaatactg actgcaacac ctaacattct ggaagcattg
     1801 tttccgagtc tggtaataga tggtgcgata aatccaatga tgatcagcgg aacagaaaag
     1861 ctgatcagct gaccaagaat atacttcgat gttacaacca cattcagtac tgcagtcgta
     1921 atggcagatt catctattat attaagaaga agtccgatac agattccaaa agccacgccg
     1981 acaagcagtc tgaagggcag actgtgtaga aaaaagtttt ttttcataat aatccccctt
     2041 tttctgtcaa ttataatata taatgatgtc atggtcaaaa ggtattttat atcgaaacag
     2101 gagagagtta tgtacagaaa aatttatcaa attttggaag aaacaggaaa agtaaaaaca
     2161 gcaatggttt taaacgggaa tcataagggc gaaaaatgtc tgataaaag
//
LOCUS       DBMDXG010000374         2189 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10448_length_2189_cov_1.687739, whole genome shotgun sequence.
ACCESSION   DBMDXG010000374 DBMDXG010000000
VERSION     DBMDXG010000374.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10448_length_2189_cov_1.687739"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            163..1398
                     /gene="hflX"
                     /locus_tag="ACZPD3_09245"
     CDS             163..1398
                     /gene="hflX"
                     /locus_tag="ACZPD3_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR1416208.1"
                     /translation="MEEFGKLEERVVLVGVQTDDHDNVEESLDELKELASTAGAVTVG
                     RIIQNRESVHPGTYIGKGKIEEVRALVYAMDATGIICDDELSPAQLNNLERELDCKVM
                     DRTLLILDIFAARAITSEGKIQVELAQLRYRSARLVGLRESLSRLGGGIGTRGPGEKK
                     LETDRRLIRTRISALKQELSQVEKHRELIRSSRARGNMKTAAIVGYTNAGKSTLLNTL
                     TGSEVLSEDKLFATLDPTTRLLNLKDGQQILLTDTVGFIHKLPHHLVEAFKSTLEEAK
                     YADYIIHVVDSSNPQAEMQMHVVYETLKELGVMGKKIITLFNKQDAPGACVLRDFKSD
                     YTLKVSAKTGEGLADLNDLLEKLLAEEQIYVERLFPYQEAGKIQLIREYGQLISEEYT
                     EEGIAVKARVPKEIYARVV"
     gene            1614..>2189
                     /gene="nrdD"
                     /locus_tag="ACZPD3_09250"
     CDS             1614..>2189
                     /gene="nrdD"
                     /locus_tag="ACZPD3_09250"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR1416209.1"
                     /translation="MFQVVKRDGEVDEFKIGKITAAIHKAFDAKEKNYSEEMIDLLGL
                     RVTSDFQKKITDNKITVEEIQDSVENVLIQAGYADVAKAYILYRKQREKVRNMKSTIL
                     DYKEIVNSYVKVEDWRVKENSTVTYSVGGLILSNSGAVTANYWLSEIYDNEIADAHRN
                     ADIHIHDLSMLTGYCAGWSLKQLIQEGLGGIE"
BASE COUNT          692 a          385 c          572 g          540 t
ORIGIN      
        1 caattgcacg tgcctcatag aatgcaatca tgtcttccga ttcagactgc tttgcagaaa
       61 gcacaggaat atctggaatt atacccggaa gataaaactg ttaagaagga acttgcattt
      121 cttaaaacgc gtgtgtaata tccatctgac tgaggagaat ttatggaaga atttggaaag
      181 cttgaagaac gggtggttct tgtgggagtg cagacggatg atcatgataa tgtggaagaa
      241 tccctggatg aactgaagga gcttgcatca actgcgggag ctgttacagt gggaagaatc
      301 attcagaatc gtgagtctgt gcatcccgga acttatattg gaaaaggaaa gatagaggaa
      361 gtacgtgcac tggtatatgc tatggatgca accggaatta tctgtgatga cgaactttct
      421 cctgcacagc tgaataatct ggagcgggaa ctagactgta aggtgatgga taggacatta
      481 ttgattctgg acatttttgc agcgagagct ataaccagtg agggtaaaat acaggtagag
      541 cttgcacagc ttcgctacag atctgcccgt ctggtcggtc tcagggaatc tctttcccgt
      601 ctgggaggtg gaatcggaac cagaggtccg ggagagaaaa agctggagac agaccgtcgt
      661 cttattcgta caaggatctc tgctttgaag caggaattat cccaggtgga gaaacacagg
      721 gaactgatcc gttccagccg ggccagaggc aatatgaaga ctgctgcgat cgtaggatat
      781 acaaatgcgg gaaaatctac gctgctgaac acactgacag gctcagaggt tttatcagag
      841 gataaactgt ttgctacact ggatccaact accagacttt tgaatttgaa ggacggacag
      901 cagattcttc tcacagatac ggttggattt atccataagc ttccgcatca tctggtcgaa
      961 gcattcaaga gcacattgga agaggccaaa tatgcagatt atattatcca tgtggtagat
     1021 tcttccaatc cgcaggcaga gatgcagatg catgtggtat atgagacatt aaaagaactg
     1081 ggagtgatgg gaaagaagat catcacactc tttaataagc aggatgcgcc gggggcatgc
     1141 gtactgcgag attttaaatc agactatacg ctgaaggtct ctgcaaagac aggcgagggt
     1201 cttgcagatc tgaatgatct tctggagaag cttcttgctg aagaacagat ttatgtggag
     1261 cgccttttcc cttatcagga agcaggaaaa atccagctga tccgcgaata tgggcagctt
     1321 atctcagagg aatacacaga ggaagggatt gctgtgaagg caagggtgcc gaaagaaatc
     1381 tacgccagag ttgtataaaa aaatattttg aaaaataaga aaataccata tattgtgcct
     1441 tttgcgaaaa cgaggaacat tatgtggtat tttgcgtgtt gacgaaccgg tgatactctg
     1501 atacaatgaa cctacagtgt tactcagagc aaagtggaca gtaacgacag aaatatctcc
     1561 ggtaagaata tgcaggagaa acagattaac aagagaaaat gggggcatta gatatgtttc
     1621 aggtagtaaa acgtgatggc gaagtggatg agtttaaaat aggcaagatc acagcggcta
     1681 tccataaagc ctttgatgca aaagagaaaa attatagcga agagatgatt gatctcttag
     1741 ggctgcgtgt tacttctgat tttcagaaaa agattacaga taataagatt acagtagaag
     1801 aaattcagga cagtgtggag aatgtgctga tccaggccgg atatgcagat gtggcaaaag
     1861 cctacattct ttaccgcaag cagagggaaa aggttcgcaa catgaagtca acgattctgg
     1921 attacaaaga aatcgtaaac agttatgtga aggttgagga ctggcgtgta aaggagaact
     1981 ccacagttac ttattcagtt ggaggtctga tcttaagcaa ctccggtgca gttactgcaa
     2041 attactggct gtctgagatt tatgacaatg aaatcgcaga tgcacacaga aatgcggata
     2101 ttcatattca tgacttgtcc atgctgaccg gatattgtgc aggatggtca ctgaaacagc
     2161 tgatccagga gggacttggc ggtattgaa
//
LOCUS       DBMDXG010000375         2178 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10516_length_2178_cov_1.628310, whole genome shotgun sequence.
ACCESSION   DBMDXG010000375 DBMDXG010000000
VERSION     DBMDXG010000375.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10516_length_2178_cov_1.628310"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(163..1041)
                     /gene="hslO"
                     /locus_tag="ACZPD3_09255"
     CDS             complement(163..1041)
                     /gene="hslO"
                     /locus_tag="ACZPD3_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR1416210.1"
                     /translation="MNDYIIRAIAANDQIRAFAAVTTETVETARQDHNTSPVATAALG
                     RLLTAGAMMGTMMKGDKDILTLQIKAGGPLEGITVTADSKGRVKGYVGNPDVCIPANS
                     KGKLDVAGAVGVGFMNVIKDMGLKEPYVGQVALQTSEIAEDLTYYFATSEQVPSAVGL
                     GVLMNKDNTVRQAGGFIVQLMPFAEESTIAKLEENVQKITSVTNLLEEGHTPESLLEK
                     VLEGFDMEINEKVPTEFYCNCSRERVEKALISIGRKELNEMIQEGKSIEMNCHFCNKN
                     YEFTVEELKEILRKCK"
     gene            complement(1159..1971)
                     /locus_tag="ACZPD3_09260"
     CDS             complement(1159..1971)
                     /locus_tag="ACZPD3_09260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HIR1416211.1"
                     /translation="MESYGRFAGVYDVFMDNVNYREWADYIIETLAQDGIRDGLVLEL
                     GCGTGTVTEMLADAGYDMIGIDNSEEMLAEAMEKRAESGHDILYLLQDMQDFELYGTV
                     RAVISVCDSMNYLTDEEDLEYLFALVNNYLDPGGLFIFDMNTVHKYRDVIGDTTIAED
                     REDGSFIWENSYDRENALNVYELALFLPREDGLYEKCEEEHVQKAYSIEAIKAMIVKA
                     GMELVAVCDAYTHNPGDENCERLTFVAREHGKSAQNGYHGRIPEKPETHRAD"
     gene            2077..>2178
                     /locus_tag="ACZPD3_09265"
     CDS             2077..>2178
                     /locus_tag="ACZPD3_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIR1416212.1"
                     /translation="MVSVSTKKDAEVIVKPLADGGEGTTDALIEGLKG"
BASE COUNT          550 a          592 c          356 g          680 t
ORIGIN      
        1 tccatctcat ggatcagtct gataatctca tttccattgg cattgcagtc tgcaactgtg
       61 atataaggtg ttgcggctgc tgttttgata taaccgtttt tcattttcta cctcttcttc
      121 tctgtgctcc ggcagatcca tctgtcagta cattggcatc tcttatttac atttacgcag
      181 gatttctttc agttcttcca ctgtaaattc ataattctta ttacagaaat ggcagttcat
      241 ctcaatggac ttaccttcct ggatcatttc attgagttct tttcttccaa tgctgatcag
      301 agctttctcc actctttctc tgctgcagtt acagtaaaat tccgtaggaa ctttctcatt
      361 gatctccata tcaaagccct ccagaacttt ctcaagcaga ctctcaggag tatgcccctc
      421 ttccagaaga tttgtgacag atgtgatttt ctgcacattt tcctcaagct tcgcaattgt
      481 gctttcttcc gcaaacggca tcagctgcac gataaatccg ccagcctgac gtactgtatt
      541 atctttattc ataagaactc caagaccaac tgctgatggt acctgttcac ttgtagcaaa
      601 ataataggta agatcttctg cgatttcgct ggtctgaagc gcaacctgtc caacataagg
      661 ctctttcagt cccatatcct tgatcacgtt cataaatcct acaccgacag cacctgcaac
      721 atccagtttt cccttgctgt ttgccgggat acatacatca ggatttccca catatccctt
      781 tacacgacct ttggaatctg ctgtcacagt aattccttcc agaggacctc ctgccttaat
      841 ctgaagtgtg aggatgtcct tatccccctt catcatggta cccatcatgg cacctgctgt
      901 aagaagacgt cccagtgctg ctgtggctac cgggcttgta ttgtgatcct gtctcgctgt
      961 ttctactgtt tctgtagtaa ctgccgcaaa tgcccgaatc tgatcattcg ctgcaattgc
     1021 tctgataata taatcgttca ttcgtttatt actcctgtta tttctttcat attattttca
     1081 ttctgtccat ggcttaacat caaatcaata gcaatccccg taaacaatgc attaattaag
     1141 aggcctgcta taatcgcatc aatccgcgcg atgtgtctca ggtttctccg gaatccttcc
     1201 atgatatccg ttctgtgcac tttttccatg ctctcttgca acaaaagtca gacgttcaca
     1261 attctcatca cccggattat gagtatatgc atcacagact gccaccagtt ccattcctgc
     1321 tttcacaatc atagccttga ttgcttctat ggaatatgct ttctgtacat gttcctcctc
     1381 acacttctca tacaatccgt cttctctcgg aagaaaaagt gccagttcat atacattcag
     1441 ggcattttcc ctgtcataac tgttctccca gataaagctt ccgtcctctc tgtcctcagc
     1501 aatagtcgta tcgccgatca catcccggta tttatggacc gtattcatat caaaaatgaa
     1561 cagcccgccc ggatccagat aattatttac cagcgcaaaa agatactcca gatcttcctc
     1621 gtccgtcaga taattcatac tgtcacagac actgatcaca gcacgaaccg taccatataa
     1681 ctcaaaatcc tgcatatcct gcaaaagata cagaatatca tgaccggatt ctgcccgctt
     1741 ctccatagcc tctgcaagca tctcctcaga attatcgata ccgatcatat catatccggc
     1801 atctgcaagc atctctgtca cagtacctgt tccacatccc agctccagaa ccagaccatc
     1861 cctgatcccg tcctgagcca gtgtttctat aatatagtct gcccattcac gatagtttac
     1921 attatccatg aaaacatcat aaacaccggc aaatcttcca tatgattcca ttcatcagcc
     1981 ctcattatct acctgtttta ctgttcactc cgttcacagc aacacatatt ttccctgcta
     2041 ttctaccaca acatccgttc ttatgtaaat aacaggttgg tctctgtcag cacaaaaaag
     2101 gacgcagaag tgatcgtaaa acctctggct gacggtggag agggaactac ggatgcgctg
     2161 atcgaaggtc tgaaggga
//
LOCUS       DBMDXG010000376         2171 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10548_length_2171_cov_1.997101, whole genome shotgun sequence.
ACCESSION   DBMDXG010000376 DBMDXG010000000
VERSION     DBMDXG010000376.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10548_length_2171_cov_1.997101"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(20..1141)
                     /locus_tag="ACZPD3_09270"
     CDS             complement(20..1141)
                     /locus_tag="ACZPD3_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HIR1416213.1"
                     /translation="MKVALICFSLTGQQTGERLCRGLEAAGMTAELDKKSKYLLDSIQ
                     ISTSAWAGEKFSDSDALIFIGATGIAVRSIAPYVASKKSDPAVLVVDECGKFVISLLS
                     GHLGGANELALKTAEILEAIPVVTTATDLHHRFAVDVFAKKNNCNIFNMKAAKEVSAT
                     LLAGKKVGFYSEFPTDGELPEGLIRCDEYGNSVSSMDDRSEEETQKSSDFDGTGTDCT
                     NIDCGVAVTVHTSCNPFISTTQVVPKCLTLGMGCRKDKDARGIAEAAQKVLDRSGFHK
                     EAFEQIASIDLKKEEKGILSLSQDWQIPFVTYTEEELKQVPGEFTPSPFVKKITGVDN
                     VCERSAVLASGNGRLLQRKTGENGVTTAVAAREWRIHFE"
     gene            complement(1215..1991)
                     /gene="cobM"
                     /locus_tag="ACZPD3_09275"
     CDS             complement(1215..1991)
                     /gene="cobM"
                     /locus_tag="ACZPD3_09275"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR1416214.1"
                     /translation="MVHFVGAGPGAPDLITVRGKQYLEEADVVIYAGSLVNPKLLEYT
                     KDICTIYNSAKMTLEEVIHVIEKAEAEGKTTVRLHTGDPCIYGAIREQMDILDEKNIT
                     YDYCPGVSAFCGAASALNLEYTLPDISQSVIITRMEGRTPVPSKESIQSFAAHQATMV
                     VFLSTGMLEELSRRLIEGGYTADTPAAIVYKATWPEQKTFVCTVGTLAKTAAENNITK
                     TALMIIGDTVAAAHYDRSKLYDPEFTTEFREAVKSKTHHS"
BASE COUNT          524 a          566 c          410 g          671 t
ORIGIN      
        1 gattccgatt acatatattt tactcaaaat gtatcctcca ttccctggca gccactgctg
       61 tggtcacacc attttctcct gtttttctct gcagcagtct gccatttcca ctggcaagga
      121 ctgcacttct ctcacagaca ttgtccacac ctgtgatctt ttttacaaaa ggtgagggag
      181 taaattctcc aggcacctgt tttaattctt cttctgtata agtcacaaaa ggaatctgcc
      241 agtcctgtga caatgacagg attccctttt cttctttctt cagatcgatg cttgcgatct
      301 gctcaaatgc ttctttatga aatccggatc tgtccagcac cttctgcgct gcctctgcga
      361 ttccacgggc atccttgtct ttcctgcagc ccatacccag agtcagacat ttcggaacta
      421 cctgtgtggt agaaataaaa ggattacagg aagtatgtac agtaactgca actccgcagt
      481 ctatattcgt acaatctgtc cctgttccat caaaatcaga gcttttctgt gtttcctctt
      541 ctgatctgtc atccatactg ctgacagaat tcccatattc gtcacaccgg atcagcccct
      601 ccggcagttc accgtccgtt ggaaactcac tgtaaaatcc aactttcttt cctgccagaa
      661 gtgtagcaga gacttccttt gctgctttca tgttaaagat attgcaattg tttttctttg
      721 caaaaacatc tactgcaaaa cggtgatgca ggtctgtggc tgttgtgacc actggtattg
      781 cttccaaaat ttctgctgtt tttagcgcca gttcattggc accgcccaga tgtcctgaca
      841 gaagggaaat gacaaacttt ccacactcat ctacgaccag caccgcaggg tctgacttct
      901 ttgacgctac ataaggcgca atgctgcgga cggcaattcc ggtggcaccg ataaagatca
      961 gtgcatctga atcagaaaat ttctctcctg cccatgcaga ggtactgatc tgaatagaat
     1021 ccagaagata tttacttttt ttatccagtt ctgctgtcat accggcagct tccagacccc
     1081 ggcataatct ctccccggtc tgctgaccgg taaggctgaa gcatataagt gcaaccttca
     1141 ctgttatatt ccttctcttt ctcctgttta cagtaatctc ataaacgata gcaattcctt
     1201 aaggttacca gattctatga gtgatgtgtt tttgatttca cagcctcacg aaattctgta
     1261 gtaaactccg gatcatacag cttggagcgg tcatagtgtg ctgctgcaac tgtatctccg
     1321 atgatcatca gagcagtttt tgtaatatta ttttctgcag ccgtttttgc cagtgttccc
     1381 actgtgcaga cgaaggtctt ctgttccggc caggttgcct tatatacaat ggctgctggt
     1441 gtatctgctg tataaccgcc ttcaataaga cgtctgctca gctcttccag cattcctgta
     1501 cttaagaata caaccatagt tgcctgatga gccgcaaatg actgaatgct ttccttggac
     1561 ggaaccggag ttctgccttc cattcgtgta atgatcacac tctgagaaat atccggcaga
     1621 gtatattcca gattcagggc acttgcagca ccgcagaatg cactgacacc cggacagtaa
     1681 tcataagtga tatttttctc atccagaata tccatctgtt cacggatcgc accgtaaata
     1741 catggatctc cggtatgaag gcggacagtt gtctttccct ctgcttccgc cttctcgatc
     1801 acatggatca cttcctccag tgtcattttt gcactgttgt agatggtgca gatgtccttt
     1861 gtatattcca gaagctttgg atttaccagt gatcctgcat agatcaccac atctgcttcc
     1921 tccagatact gttttcctct tactgtgata agatccggtg ctcccggacc tgctcctacg
     1981 aaatgtacca taatctatca ttctccttat tataaattta tttcaactgt tcaatacttt
     2041 cacagttgtg agtcaaaatc cattcaagcg gatattcgca atccgcttcg ctacttgctc
     2101 ataaccgaat aactgctccg cagtttatca gaaggagctt gcactcctcc tgatacaagc
     2161 aagtccccac c
//
LOCUS       DBMDXG010000377         2170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10552_length_2170_cov_1.500725, whole genome shotgun sequence.
ACCESSION   DBMDXG010000377 DBMDXG010000000
VERSION     DBMDXG010000377.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10552_length_2170_cov_1.500725"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="ACZPD3_09280"
     CDS             complement(<1..798)
                     /locus_tag="ACZPD3_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1416215.1"
                     /translation="MKKKIATILTAAVIGATAFTTIVSAASGDITVVSREDGSGTRGA
                     FVELFGIEEEKDGEKVDMTTDEASVTNSTSVMMTTVAGDENAIGYISLGSLDDTVKAV
                     KIDGVEATVDNVSNDSYKIARPFNILTSDKESDAAKDFVNYIMSSDGQKIVEDNGYIK
                     EAADAKAYEAADGVSGKVVVAGSSSVTPVMEKLAEGYEAVNKDVTVEVQQSDSTTGVN
                     MAAEGTADIGMASRDLKDEEKDLGLTATVIARDGIAVIVNKDNDVDEL"
     gene            1114..1506
                     /locus_tag="ACZPD3_09285"
     CDS             1114..1506
                     /locus_tag="ACZPD3_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR1416216.1"
                     /translation="MKNLRCEKAVEKKHNGYNCAQAVACSFCKEASMDEDTLKKITQG
                     FGAGLGTMAGTCGAISGAAVVAGLINQDKAGTSQTVRSVMNQFKQQNGTVICKDLKGV
                     ETGKVIRSCDDCVRDAVKFLEDALKSEN"
     gene            complement(1644..2129)
                     /locus_tag="ACZPD3_09290"
     CDS             complement(1644..2129)
                     /locus_tag="ACZPD3_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416217.1"
                     /translation="MEKCYSIWICAEDVPKKLQNTLTEYSFSKKDIIGVADEPEEDYD
                     LLTVIIIRLGKETEEKGIFDYLKGLFTGDIKRIQRYSHIEWSEPFQEEASKMTGFGDM
                     IYERGIQQGMQQGIQQGRHEGMILGALMSGKTPEEVSKMLNLPLEEIKKVQEQQMTVN
                     K"
BASE COUNT          582 a          476 c          376 g          736 t
ORIGIN      
        1 aagttcatca acatcattgt ctttgtttac aataactgcg ataccatctc ttgcgataac
       61 tgtagctgta agaccaaggt ctttctcttc atctttcagg tcacgggatg ccataccgat
      121 atctgctgtt ccttctgctg ccatgtttac acctgttgtg gaatcactct gctgaacttc
      181 tacagttacg tctttgttta ctgcttcata gccttctgca agtttctcca taaccggtgt
      241 tactgaagaa gaacctgcaa ctactacttt accggaaact ccgtctgctg cttcataagc
      301 cttagcatct gctgcttctt tgatatatcc gttgtcctct acgatcttct gtccgtcaga
      361 gctcataatg tagtttacaa aatcctttgc tgcatcgctt tctttatcag atgtaaggat
      421 gttgaatgga cgtgcgatct tgtaagaatc attggaaaca ttatctacag tagcttctac
      481 accgtcgatc ttaactgcct ttactgtatc atccagagaa ccaagtgaga tataaccgat
      541 tgcattttca tcacctgcaa cagttgtcat cataacagat gtgctgtttg ttacacttgc
      601 ttcatctgta gtcatatcta ctttctcacc gtctttttct tcttcaattc caaacagttc
      661 tacgaatgct ccacgtgtac cggaaccatc ttcacgggat acaactgtga tgtctccgct
      721 tgctgcactt acgattgttg tgaaagctgt tgctccgata actgctgctg taaggattgt
      781 tgcgattttt ttcttcatga taattttctc ctcttcccat gagccatgtt tttaatataa
      841 agtgaaaggc tcatttcctt tttaatatct ttatgtttta taacttgttg cctcatttac
      901 aagtgctatt atagaaatcc aaagttaaat gtaaaaccac gctaaagtaa aatctttgta
      961 aattgaaaat ttttgtgaag gttgtaaaaa caggttcaag gtgctatcat aaactatatc
     1021 ttcaagtccc cacccactgc ttattacttt tcgcaagtca agcaggggac ttacttgatt
     1081 cggttaaaat ttatacacag gaggatttta tatatgaaaa atttaagatg tgaaaaagca
     1141 gtagaaaaga aacataacgg ctataactgt gcacaggctg tggcctgttc tttctgtaaa
     1201 gaggcatcta tggatgagga tacactcaaa aagatcacac agggattcgg tgcaggtctt
     1261 ggcactatgg ccggtacatg cggagcaatc tccggtgctg cagttgtagc tggacttatt
     1321 aaccaggaca aagccggaac ctctcagact gtccgctctg tcatgaatca gtttaaacag
     1381 cagaatggaa ctgtaatatg taaagactta aagggtgtgg aaaccggaaa ggtaatccgc
     1441 tcctgtgatg actgtgtcag agatgcggtg aaattcctgg aagacgcatt aaaatcagaa
     1501 aattgatttc aggcagatat tcgcaatgca taaataccaa cgcaaaagga gctatgcacg
     1561 tataatgtgc atagcctctt ttatgattag cttgattaat gctcccagtt cccaattaat
     1621 tttatatctg taatttactt tcactacttg ttcacagtca tctgctgttc ctgaactttt
     1681 tttatttctt ccagaggcaa gttcaacatc ttcgaaactt cttctggagt ttttccactc
     1741 atgagagccc ccagaatcat tccctcatga cggccttgct gtattccttg ttgcattcct
     1801 tgctgtattc ctctttcata aatcatatct ccaaatcctg tcattttgct cgcctcctcc
     1861 tgaaatggtt ctgaccattc tatatgtgaa tatctttgta ttctctttat atcaccagta
     1921 aaaagccctt tcagataatc aaatattcct ttttcttctg tttcttttcc cagtcggatt
     1981 ataattactg ttaagagatc ataatcctcc tcaggttcat ctgctacccc tataatatcc
     2041 ttcttcgaaa aagagtattc cgtcaatgta ttctgcagtt tctttggcac atcctctgca
     2101 cagatccata tggaataaca tttttccaac tttgaataat ctgtagtctg cgtaatagta
     2161 cttaactgag
//
LOCUS       DBMDXG010000378         2168 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10565_length_2168_cov_1.367683, whole genome shotgun sequence.
ACCESSION   DBMDXG010000378 DBMDXG010000000
VERSION     DBMDXG010000378.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10565_length_2168_cov_1.367683"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="ACZPD3_09295"
     CDS             complement(<1..392)
                     /locus_tag="ACZPD3_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIR1416218.1"
                     /translation="MKAFLILEDGHVFKGTSIGSTRDVISEIVFNTSMTGYLEVMTDP
                     SYAGQAVCMTYPLIGNYGICYDDQESSKPWVDGFIVRELSRVPSNFRSVDTIQHFLTK
                     HDIPGIAGIDTRALTKILREKGTMNGMIT"
     gene            804..887
                     /locus_tag="ACZPD3_09300"
     tRNA            804..887
                     /locus_tag="ACZPD3_09300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:838..840,aa:Leu,seq:cag)
     gene            complement(940..2067)
                     /locus_tag="ACZPD3_09305"
     CDS             complement(940..2067)
                     /locus_tag="ACZPD3_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR1416219.1"
                     /translation="MKYIRKSFFLFWLIAVMLFGTVSASAASAKPETPVLSGTAAGNR
                     VTLNWNKVKKASGYQIFLYYKAYGKYKCVERIKNRNITSFTLTGSEDKLYTYKIRSYL
                     KQGNKTLYSPSSKALEIKTAPGKPVITRIRVREESGTLIKWKKIKTAEGYQIFRSESE
                     DRGYKRINIVSGNTTFSYADTGAVSGKTYYYRIRAYVRNQGNVVYSELSDPAEAVMRK
                     TIMIGDSRTDMMKDVVENDNITWICEVGMGYKWLRDTALKTLQEQMKGNEDIFVWLGV
                     NDVYNISNYISLLNEEIPKWKAQGADAYIVAVGQVTKDPYVTNEEIEDFNARMKKEVA
                     GAKYADLYSYLKKQGYKTTDGTHYDNETTWKIYRYLMSFVS"
BASE COUNT          588 a          486 c          361 g          733 t
ORIGIN      
        1 gtgatcatac cgttcatggt acctttctca cgaaggattt tcgttaaggc tctggtatca
       61 attccggcaa tacctggaat atcatgcttt gttaagaaat gctgaatcgt gtccacgctt
      121 ctgaaattac ttgggacacg ggataattcg cggacaataa agccgtccac ccatggcttt
      181 gatgactcct ggtcatcata acagattccg taattcccaa tgaggggata agtcatacat
      241 actgcctgtc ctgcatagga agggtcggtc attacttcca gataaccggt catggaagta
      301 ttaaagacga tttcgctgat aacgtctctc gttgaaccaa tgcttgtacc tttgaacaca
      361 tggccatctt ctaatatcag aaatgctttc atacaatctc cctttcttat ctttatattg
      421 tgtctatcac ttattttttc ctaaatcttc aagattgtat cacagcaaag tgcttttttc
      481 aaccttataa ggtactaaaa ataattaagt tgatattaaa aacttttttg taatttcagg
      541 cagatccaat cacatgcatt cactgctttc tcacatccaa atacctctat aaaatttaaa
      601 tcatcctctt ctctgtgcaa ctctacaccg gataactgct ccgcagttta tcagaagaag
      661 cttacacttc tacgtaacaa attgtgcgtt ttgatgatac tgtaaaaaaa attaaaaaaa
      721 tttcaaaaaa gctattgact tttatccata catcggttat tataaatgag cagtcgcgag
      781 agagcgattg ataaataaga aatgcagaag tggcggaatt ggcagacgcg caggcttcag
      841 gtgcctgtgg tagcaatatc gtgtgggttc aagtcccatc ttctgcatta tttttttact
      901 caaaaaagga aactgataca gtttcctttt tttctgttct tatgacacaa aagacatcag
      961 ataacggtaa atcttccatg tggtttcatt atcgtaatga gttccgtctg ttgtcttata
     1021 tccctgcttt ttcagataag aataaagatc tgcatatttc gcaccggcaa cttctttttt
     1081 cattctggca ttgaaatctt cgatttcctc atttgttaca taaggatctt tggttacctg
     1141 tcccactgcc acaatatagg catccgctcc ctgcgctttc cattttggaa tctcctcatt
     1201 cagcagactg atataatttg aaatattgta tacatcatta acaccaagcc agacaaaaat
     1261 atcttcattt cctttcatct gctcctgcaa ggtcttcagg gcagtatctc taagccattt
     1321 ataccccatt ccaacctcac atatccatgt gatgttatcg ttctccacaa catctttcat
     1381 catatctgtt ctggaatctc cgatcataat agtctttcgc attaccgcct ctgccggatc
     1441 actcagttca gaatatacaa catttccctg atttcgtaca tatgcccgta ttctgtaata
     1501 ataagtcttt cctgaaacag cacctgtatc tgcataactg aatgttgtat tgccggaaac
     1561 tatattgatt cttttatatc ctctgtcctc ggattctgac cggaatatct gatatccttc
     1621 tgcagttttg atttttttcc atttgatcag ggttccgctt tcttctctca ctcttatccg
     1681 ggtgatcaca ggttttccgg gagctgtttt tatctccagt gctttcgatg atgggctgta
     1741 cagagttttg tttccctgtt tcagataaga acgtatctta taagtgtaga gcttatcttc
     1801 agaacctgtc agtgtaaaac tggtaatgtt cctgttcttg atcctttcca cgcatttgta
     1861 ttttccgtag gctttataat acagaaaaat ctgatagccg gaggccttct ttactttatt
     1921 ccagttcaga gtcactctgt ttccggctgc tgttccggac aataccggtg tttccggttt
     1981 tgcagatgct gcggatgccg atactgttcc aaacagcatt accgctatca accaaaataa
     2041 aaagaaactc tttctgatat atttcacatg acattcctcc catctataac aattcatcta
     2101 tagcaatacc ggactgtcca cagtatttta cggaattgcc ataatcaaag agccggctct
     2161 gtaaaaga
//
LOCUS       DBMDXG010000379         2158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10618_length_2158_cov_1.401556, whole genome shotgun sequence.
ACCESSION   DBMDXG010000379 DBMDXG010000000
VERSION     DBMDXG010000379.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10618_length_2158_cov_1.401556"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="ACZPD3_09310"
     CDS             complement(<1..135)
                     /locus_tag="ACZPD3_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR1416220.1"
                     /translation="MKSLWNNFKVAFAMFSKIPMPRADWTKENMKYMFCFFPFIGTVI
                     G"
     gene            complement(181..723)
                     /locus_tag="ACZPD3_09315"
     CDS             complement(181..723)
                     /locus_tag="ACZPD3_09315"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR1416221.1"
                     /translation="MLTLVTGGSGSGKSAFAEDRVLSFGDAQRIYIATMHPFDEESHK
                     RIERHQKMRAGKGFETVECYTGLKNVKLPAGCVVLLECMSNLVANEMFEEQGAHDRTV
                     SEVTKGIENLLEQAAHVVIVTNEIFSDAVVFDGDMDSYLEYLGKINQAAAQRADEVVE
                     VIYGIPVFHKSADTCSNLCK"
     gene            complement(723..1775)
                     /gene="cobT"
                     /locus_tag="ACZPD3_09320"
     CDS             complement(723..1775)
                     /gene="cobT"
                     /locus_tag="ACZPD3_09320"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR1416222.1"
                     /translation="MTLEEAIAKIKPLDHNAMEIAQKRWDSIAKPLHSLGKLETLLIQ
                     IAGITGNAEVDLSRRGLIAMCADNGVVEEGVTQTGQEVTAIVAENFLKYDTSVGVMCK
                     QNHAEIFPVDMGMVTDTKVRTDHKIAYGTQNMTKGPAMTREQAVKGLEAGIDMVRELN
                     DKGYRILATGEMGIGNTTTSSAVASVLLKQPVEEMTGRGAGLTSEGLVRKINAIKKAI
                     ALNEPDPEDAIDVLAKVGGLDIAGMAGVFLGGAVYGIPVVMDGFISCVSALIAMRICP
                     AARDYILASHVSNEPAAHLILENMGKEAIIHADMCLGEGTGAVALFPILDLAAAVYHS
                     MSTFDDIHVEQYEELK"
     gene            complement(1800..>2158)
                     /locus_tag="ACZPD3_09325"
     CDS             complement(1800..>2158)
                     /locus_tag="ACZPD3_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR1416223.1"
                     /translation="GCTTMVNLVSFFILRKLKVELNIANIISIILAILFAYVVNSKYV
                     FQDKCETLKDHVQPFCKFVSARLITMVIEVGGVWLLVSVMGLNDMIGKFLTQFIVLIL
                     NYVFSKFFVFTTGKSK"
BASE COUNT          576 a          598 c          375 g          609 t
ORIGIN      
        1 gccgatcaca gttccgataa acggaaaaaa acagaacata tacttcatat tttcctttgt
       61 ccagtcagcg cggggcatgg gaatcttaga aaacatggca aatgccacct taaaattatt
      121 ccataaagat ttcattgatc aattcctctt tttcatagta tgtcaatcct gtcaaatata
      181 ttatttgcaa agattgctgc aggtatctgc agatttgtga aagactggta tcccatatat
      241 aacttccacc acttcatcag ctctctgcgc agcagcctga ttgatttttc ccagatattc
      301 cagataagaa tccatatctc catcaaacac aaccgcatct gaaaaaatct catttgtaac
      361 gattaccaca tgagcagcct gttccagcag attctcaatc ccttttgtaa cttccgaaac
      421 tgtccggtca tgggctccct gttcttcaaa catttcattt gccaccagat tcgacataca
      481 ttccagaagc accacacatc cggcaggcag tttcacattt ttgagccctg tatagcactc
      541 aactgtctcg aaaccttttc ctgcacgcat cttttggtgt ctctcgatcc ttttatggct
      601 ctcttcatca aaagggtgca tggtcgctat ataaatcctc tgtgcatccc cgaaagaaag
      661 caccctgtct tccgcaaaag cagattttcc gctcccgctt ccccctgtca caagcgtcag
      721 cattatttca gctcctcata ctgttccaca tggatatcgt caaaagtact catagaatga
      781 taaaccgctg ctgccagatc aaggatcggg aataaagcta ctgctccggt tccctcgccc
      841 agacacatat ccgcatgaat gattgcctct ttccccatat tctcaaggat cagatgggca
      901 gccggctcat ttgatacatg ggatgcaaga atataatccc tcgcagccgg gcagatacgc
      961 atggcgatca gcgcagaaac acaggaaata aatccgtcca tgactacagg aataccataa
     1021 accgcacctc ccagaaagac acctgccatt ccagcgatat caagaccgcc tacctttgcc
     1081 agaacatcaa tggcatcctc aggatccggt tcatttaatg caatggcttt tttgatcgca
     1141 ttgatcttgc gcacaagtcc ctcgctggtc agacctgcgc cgcgccctgt catctcttcc
     1201 accggctgtt tcagcaacac agaagcaact gcactgctgg tggttgtatt tccgattccc
     1261 atttcccctg ttgccagaat ccggtagccc ttatcattca gttcccttac catgtcaatg
     1321 cctgcttcaa gcccctttac agcctgttct cttgtcatgg caggaccctt ggtcatattc
     1381 tgtgtaccat aggcaatttt atggtctgtg cggacttttg tatccgtcac catccccata
     1441 tccaccggaa aaatctccgc atggttctgc ttacacatca caccgacaga tgtgtcatat
     1501 ttcagaaaat tctcggcaac gatggcagta acttcctgtc cggtctgtgt cacaccctct
     1561 tctaccacgc cattatccgc gcacatggca atcagacctc tcctggaaag atccacctct
     1621 gcgtttcctg taattcccgc gatctggatc aaaagagtct ccagttttcc aagagaatgc
     1681 agtggtttcg caatactgtc ccatcttttc tgtgcaatct ccatggcatt gtgatctaat
     1741 ggcttaatct ttgcaatagc ttcttccaga gtcatgagtt ttccctctct tttccttcat
     1801 tattttgatt tccctgttgt aaacacaaaa aatttactga atacataatt cagtatcaac
     1861 acaataaact gtgtcaggaa tttcccgatc atatcattaa gccccatcac tgacacaagc
     1921 agccatacac cgccaacctc gatcaccata gtgatcagtc tggcactgac aaatttgcag
     1981 aatggctgaa catgatcctt caatgtctcg catttatcct gaaaaacata cttcgaattt
     2041 accacatagg caaacaggat cgccaggatg attgaaataa tattggcgat attcagctcc
     2101 accttcagct ttctgagaat gaaaaagctg accagattta ccatagtggt acatccgc
//
LOCUS       DBMDXG010000380         2152 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10656_length_2152_cov_1.184788, whole genome shotgun sequence.
ACCESSION   DBMDXG010000380 DBMDXG010000000
VERSION     DBMDXG010000380.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10656_length_2152_cov_1.184788"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="ACZPD3_09330"
     CDS             complement(<1..493)
                     /locus_tag="ACZPD3_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1416224.1"
                     /translation="MKNLDKEEFRVKLEEINHLVEQKDYKGAMNVVDSIDWRRVKNVR
                     TLCVVGEIYAANKRYEDSKEIFLLAYHRASIGKNILYRLVEVSLKLGQVSEAVEFYQE
                     YREVAPNDNTQYILKYKILKVKKAPLAEQIKVLEDYKEKEFTEKWSYELAKLYYQDGD
                     KEKC"
     gene            complement(546..1652)
                     /gene="recF"
                     /locus_tag="ACZPD3_09335"
     CDS             complement(546..1652)
                     /gene="recF"
                     /locus_tag="ACZPD3_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541432.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR1416225.1"
                     /translation="MAYIESIKLSNFRNYESLELSFDKGTNIFYGDNAQGKTNILESV
                     YLCGTSKSHKGSKDREIIRFEQDEAHIRMMVGKDSMSYKIDMHLRKNKAKGVAINGLP
                     IKKARELLGVVNLVFFSPEDLNIIKNGPGERRRFMDAELCQLDKLYLTDLAGYNHVLN
                     QRNKLLKDMYKQPDLGATLDVWDMQLVNYGRKIIGKRREFVESLNEIIKEIHRNLTGG
                     IENIEVIYEPSVESEALEESLFRNRDRDLRMKMTSSGPHRDDLMVAVNGIDIRKYGSQ
                     GQQRTAALSLKLSEIYLVEKIIHDKPVLLLDDVLSELDSSRQNYLLESIHDIQTMITC
                     TGLDDFVSHQFTINKVFHVVAGHVYQPIGGPADK"
     gene            complement(1654..1869)
                     /locus_tag="ACZPD3_09340"
     CDS             complement(1654..1869)
                     /locus_tag="ACZPD3_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR1416226.1"
                     /translation="MEIRIRDEFIKLGQALKLAELVSDGVEAKYVINDGLVKVNGEVD
                     TRRGRKVYGGDVISYNGQDVKVLSGNE"
     gene            complement(2003..>2152)
                     /locus_tag="ACZPD3_09345"
     CDS             complement(2003..>2152)
                     /locus_tag="ACZPD3_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR1416227.1"
                     /translation="FLIDALKVIDDETIDIYLVNPKAPCFIRDEEETYTYLILPVNIN
                     QNQAR"
BASE COUNT          538 a          551 c          380 g          683 t
ORIGIN      
        1 gacatttctc tttgtcacca tcctgatagt acagttttgc aagctcgtaa gaccattttt
       61 cggtaaattc tttctcctta taatcctcca gaaccttgat ctgctctgca agaggagcct
      121 tttttacttt caggatcttg tatttcagga tgtactgcgt attatcattt ggcgcaactt
      181 ccctgtactc ctgataaaat tccactgcct cggaaacctg tcccagcttc agggaaactt
      241 ctacaagacg ataaagaatg ttcttaccaa tggaggctct gtgataagcc agcaggaaaa
      301 tttccttgct gtcctcatag cgtttattgg cagcatagat ttcaccaacc acacaaaggg
      361 tacgaacatt tttcacacgg cgccagtcaa tgctgtctac aacattcatg gctcccttat
      421 agtctttctg ttctactaaa tggttaatct cttcaagttt aacccgaaat tcctctttgt
      481 ccaaattttt caccaacttt ccatagttaa tgccagttta gcatatcgca tactacttcg
      541 caagcttatt tatctgcagg accccctatc ggctgataca catgccctgc cacaacgtga
      601 aacactttat tgatcgtaaa ctgatgactg acaaaatcat ccagaccagt acaggtgatc
      661 atggtctgaa tatcatgaat actctcaaga agataattct gacggctgct gtccagctcc
      721 gaaaggacat cgtccagaag cagaactggt ttatcatgaa tgatcttctc caccagataa
      781 atctccgaaa gtttcaggga aagggctgct gttctctgct gcccctgcga accatatttg
      841 cggatatcaa ttccgttcac agccaccatt aggtcatccc tgtgaggtcc ggacgaagtc
      901 attttcatcc gaagatcccg gtctctgttg cggaaaaggc tctcttccaa agcctcactc
      961 tccacgcttg gttcatatat cacttctata ttctcaatac caccggtaag atttctgtga
     1021 atttccttaa tgatctcatt aagggactcc acaaattctc ttcttttgcc aatgatctta
     1081 cggccataat ttaccagctg catatcccat acatccagag tcgccccaag atccggctgc
     1141 ttatacatat ccttcagcag cttatttctc tggttcagaa catgattgta tccggcaaga
     1201 tctgtgaggt atagtttatc cagctgacaa agctccgcgt ccatgaatct tcgtcgctct
     1261 cccggaccgt ttttgataat attcagatcc tccggggaaa agaaaaccag attcaccaca
     1321 ccgagaagct ctctcgcctt ctttatggga agcccgttga ttgccacgcc cttggcctta
     1381 tttttccgaa ggtgcatgtc aatcttataa gacatggaat cttttcccac catcatgcgg
     1441 atatgggctt catcctgttc aaagcggata atctcccggt ctttgcttcc tttatgggat
     1501 ttgctggttc cgcacagata cacagactcc aggatattgg tttttccctg tgcattatcc
     1561 ccataaaaaa tattagttcc tttgtcaaaa gaaagttcca gcgattcgta atttctgaaa
     1621 ttgcttaatt ttatggattc aatataagcc attttactcg tttccggaca gaaccttcac
     1681 atcctggcca ttataggaaa taacgtcacc gccataaacc ttgcggcctc ttctggtatc
     1741 tacttcgcca tttactttta caagtccgtc gttgatcaca tattttgcct caacaccgtc
     1801 tgatacaagt tcagcaagtt tcagtgcctg gccaagtttg ataaattcat ctctgattct
     1861 gatttccaat tttttgcctc cactcttctg gcatcacgac tgcaattcgt tttcggactg
     1921 tctccagcca ttatttgcag cccgtgttca cttccattcc tcttccaatt gtctgttacg
     1981 tttctctctg tggcaagtgt cattatcttg cctggttctg atttatgtta accggaagaa
     2041 tcagatacgt gtaagtttct tcctcatctc tgataaagca aggtgctttc gggtttacca
     2101 ggtaaatgtc gatggtctca tcatcaataa ccttcagtgc atcgatcagg aa
//
LOCUS       DBMDXG010000381         2142 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10718_length_2142_cov_1.454679, whole genome shotgun sequence.
ACCESSION   DBMDXG010000381 DBMDXG010000000
VERSION     DBMDXG010000381.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10718_length_2142_cov_1.454679"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="ACZPD3_09350"
     CDS             <1..957
                     /locus_tag="ACZPD3_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1416228.1"
                     /translation="SVNDEMISEFKHKGYLEPLEKNVMTEEVRDSFPQKYLESICEEN
                     GHIYSVPFQMDIMMFWVNQELLKQAGLDEIKNTGDFDILQKSLKNPDQYAYGDAWENT
                     HVYNSLSQFVNFWGGDYFDWTDPKTKDAARYMKSMLDEKNTSPAQFVDQYEQMEEKFI
                     RGKYGCVFMYTSALGTFLDADVYGKNKIHMAPLPVLHKKKATNIATWQYVLNNASENK
                     DAAVRFLQYAAGYEGSTEYAKIMKSYPARVDVIENEDIDLEGIDMIRKYLREYTLNAR
                     PLCENSMGAVSDMGKLFQGYVLGQCEEDSFFEQAQNCINTYY"
     gene            1045..1980
                     /locus_tag="ACZPD3_09355"
     CDS             1045..1980
                     /locus_tag="ACZPD3_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR1416229.1"
                     /translation="MQNQQPSTLKEIRVHGTQDFPCAFYQTGTRIKGNMVKHHWHEEV
                     ELLYFSGGEFCLEVNMERFNISEECFYVINPGELHSISVEKNGGCVENAVVFHMDMLS
                     SAYALDQIQTNLIQPVQNGSIQFPRCLRKNEPAFEPVRKSYEEIRNVFGGDSFRENYY
                     DGEAVTDDITRQLFIKSALLRILAVLSANNLFEVTEKNHDHRIETIKTTLTYIQENYK
                     EKIYIRDLAGLIGMNEQYFCRFFKKVIGRSPMEYVNEYRIKKAIHYLKESDLTVTEIC
                     LECGYNNLGNFLREFRKYTSTTPLQYRRHSLKETQ"
BASE COUNT          719 a          326 c          507 g          590 t
ORIGIN      
        1 tccgtgaatg atgagatgat ttctgagttt aagcataagg gatatctgga accactggaa
       61 aaaaacgtta tgacagaaga agtaagagac agctttccgc agaagtatct ggaaagcatt
      121 tgtgaagaga atggacatat atattctgtc ccttttcaga tggatattat gatgttctgg
      181 gtaaatcagg agttgctgaa acaggcagga ctcgatgaga taaagaatac cggtgatttt
      241 gatattctgc agaagagttt gaaaaatccg gatcagtacg cttatggaga tgcatgggaa
      301 aatacacatg tttataacag tctttcgcaa tttgtgaatt tctggggtgg cgattatttc
      361 gactggactg atccgaaaac caaagatgca gccagatata tgaaaagtat gctggatgag
      421 aaaaatacat cgcctgcaca gtttgtagat cagtatgagc agatggaaga gaaatttatc
      481 aggggaaaat atggatgtgt ttttatgtat acgagtgctc tgggtacatt tcttgatgct
      541 gatgtatatg ggaaaaataa aatacatatg gcaccgcttc ccgtgcttca taagaaaaaa
      601 gccactaata ttgcaacctg gcagtatgtt ttgaacaatg cttcagaaaa taaagatgca
      661 gctgtcagat ttcttcagta tgcagccggt tatgaaggca gtactgaata tgcaaaaata
      721 atgaaaagtt atcctgccag agtggatgtg atcgaaaatg aggatattga tctggaagga
      781 atagatatga tacggaaata tctccgggaa tatacattga atgcacgtcc gttatgtgaa
      841 aattccatgg gtgctgtctc agatatggga aaactgtttc agggatatgt tcttgggcaa
      901 tgtgaagagg acagtttctt tgagcaggca caaaattgta taaatactta ctattaaaaa
      961 ggcatcttcg gatgtccttt ttagttgcgg atttttgatg ccggatttat aataacaata
     1021 acaatgacaa caggaggcag cttcatgcag aatcaacaac catcaacttt aaaggaaata
     1081 cgtgtccatg gtacgcagga ctttccctgt gcattttacc agacaggaac aaggataaaa
     1141 gggaatatgg tgaaacacca ctggcatgaa gaagtagaac tgctttattt ttcgggtgga
     1201 gagttctgtc tggaagtaaa tatggaacgt ttcaacattt cagaggaatg tttttatgtt
     1261 attaatcccg gggaacttca cagtatttct gttgaaaaaa acggcggctg tgtggaaaat
     1321 gcggtggttt ttcatatgga tatgctaagc tctgcatatg ctttggatca gattcagacg
     1381 aatctgatcc agccggtaca aaatggcagt atccagtttc cgagatgtct gcgaaagaat
     1441 gaaccggcat ttgaaccggt aagaaaatct tatgaagaaa tccgtaatgt atttggagga
     1501 gattctttcc gggagaatta ttatgacggc gaggcggtta cggatgatat cacccgacag
     1561 ttgtttatta aatcagcgct gcttcggatt ctggcagttc tgtctgcgaa taatctattt
     1621 gaggtaacgg agaagaatca tgaccacagg attgagacga tcaagacaac actgacttac
     1681 atacaggaga attacaaaga gaaaatttat atacgtgatc tggcaggact gatcgggatg
     1741 aatgagcagt atttctgcag atttttcaag aaggtgattg ggcgttcgcc tatggaatat
     1801 gtgaatgagt acaggataaa aaaggccatt cattatctga aagagtctga tcttacagtt
     1861 acggaaatct gtctggaatg tggatataat aatctgggaa atttcctgag agagttcaga
     1921 aaatatacgt caacaacgcc gttacagtac cgcagacatt cattaaaaga aacacagtaa
     1981 ataagaatgc cggtaatata aaaaagtcaa aattttgtat ttttttatca aaaaaagata
     2041 agacagaagc cttggattct gatataattg ccgtaaatga aaaagagacc ttaggcgcta
     2101 aaggtaagag gcataatact gggaacaaag cagacaatag cg
//
LOCUS       DBMDXG010000382         2139 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10736_length_2139_cov_1.322866, whole genome shotgun sequence.
ACCESSION   DBMDXG010000382 DBMDXG010000000
VERSION     DBMDXG010000382.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10736_length_2139_cov_1.322866"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(77..610)
                     /locus_tag="ACZPD3_09360"
                     /pseudo
     CDS             complement(77..610)
                     /locus_tag="ACZPD3_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            complement(700..1287)
                     /locus_tag="ACZPD3_09365"
     CDS             complement(700..1287)
                     /locus_tag="ACZPD3_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR1416230.1"
                     /translation="MDIKVDHISKAYGEQQVLQDLSCVFPEGKTTCIRGRSGCGKTTL
                     IRLLLRLDVPNKGKIEGVSDRKLSAVFQEDRLCENLSAASNIRLVCTETITDRELEEA
                     YKAVALTEIWQKPVRELSGGMRRRVSILRALLAESDCVIMDEPLRGLDEKTRAKTIDY
                     ILKKTEGKTLIFVTHEEKEAVWLKADRTVDILTKH"
     gene            complement(1359..2138)
                     /locus_tag="ACZPD3_09370"
     CDS             complement(1359..2138)
                     /locus_tag="ACZPD3_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR1416231.1"
                     /translation="MTNDRAKTDNGTKYTGIRILAVLFWIAIWQFASMYLKQEILLAS
                     PVSVVRKLFELIFSGNFWQSVGFSFVRIVTGFLLAVLLGIFLAVWAYWSKTVEILTAP
                     VIAVVKSTPVASFIILCLIWIPSKNLSVFISFLMVLPVIYTNILEGIRQTDRKILEMA
                     KVFRVNLRRQIRYIYVSQVLPYFLSACRLSLGMCWKAGVAAEVIGVPSGSIGEKLYNA
                     KIYLNTPDLFAWTIVIIVISFVFEKCFLGIVSRVVYMIEHR"
BASE COUNT          626 a          537 c          336 g          640 t
ORIGIN      
        1 agattgcaca aaaaagatcc cttcatcaac catttctcac agccaacaaa gggattcacc
       61 agttatatcc atatttttaa tcaatcacat cattctgaaa tcttgacaaa tgttcataag
      121 gtaatatcag tcttcccctg aacagtccgc acccatcatt gatcagactg ttatctactg
      181 cggaaaacat atttacatcc agatctgcca gttccagctg caaaagacgc atgccccgtt
      241 cataggcttc atcgaaataa acgctctcgc cttctggaat agcatcacgt cttgcacgct
      301 catcctgctg tctcttctca tatcccagat gattcgccgg accaatttcc gcagtgtcat
      361 ccattttctg ggtacgcagc tgcacttcca tgaaagaacg ggaactgtta tcataaactg
      421 tgatatgtaa tgactgatat ccttccccgc tggttccaca gatataatcc cggtaataca
      481 tcttcacatc ttcattaaga agcgacgacg tacttttctt tactccatag gcagactcca
      541 ccgtaaaccc tcgttcttcc aggaacccgg gaagaacatt ggcaatctca taaagatagc
      601 ggatcgacgc cgtttcctga tcttctcctg gtttcagatg aataattata tacccaaaaa
      661 agcagaatga taagtaccag tttgggtttt tcattaattt taatgctttg tcaatatatc
      721 tactgttctg tcagccttta accaaactgc ttctttttct tcatgagtaa caaagatcaa
      781 cgtctttccc tccgtctttt tcaaaatata atcaattgtt tttgctctgg ttttttcatc
      841 aagacctctg agaggttcat ccatgatcac acagtctgat tcagccagca atgccctcag
      901 gatagatact ctcctgcgca ttcctccact cagttcacga accggtttct gccatatctc
      961 agtaagcgca actgctttat acgcctcctc cagttctctg tctgttattg tttctgtaca
     1021 taccagtctt atattagaag cagcgctcag attttcacac agacgatctt cctgaaatac
     1081 tgcactgagt ttcctgtcac ttaccccttc tattttcccc ttattcggaa catccagtct
     1141 caaaagaagc cgtatcagtg ttgttttgcc acatccggat cttcctctga tacaagtggt
     1201 tttcccttcc ggaaaaacac aggacaagtc ttgcagtacc tgctgttctc cgtatgcttt
     1261 actaatatga tctaccttta tatccatatc tcttctccgc ctgtaacaga cctcataaat
     1321 aatgcattta agaggattat cataaattaa aatttccgtt atctatgttc tatcatataa
     1381 actactctgc ttacaattcc aagaaaacac ttttcaaata caaaactgat cacaattatc
     1441 acaattgtcc acgcaaaaag atccggagta ttcagatata tttttgcatt atacagtttc
     1501 tcaccaatag aacctgaagg tactccgata acctctgctg ccactcctgc tttccagcac
     1561 attccaagag aaagcctgca tgctgagaga aaataaggca gtacctgcga tacataaata
     1621 tacctgatct gtcttctgag attcacacga aatacttttg ccatttccag tatcttcctg
     1681 tccgtctgtc gtattccctc aaggatattt gtatatatca ccggcagaac cataagaaag
     1741 gaaataaaaa ctgacagatt tttcgaagga atccatatca ggcacaaaat aataaaagat
     1801 gccacaggtg tagatttgac cactgcgatc acaggtgctg tcaggatttc cactgttttt
     1861 gaccaatatg cccacactgc cagaaagatt cccagaagca cagccagaag aaatcctgtc
     1921 acaatgcgta caaaactaaa tccgacagac tgccagaaat ttccgctgaa aatcagttca
     1981 aaaagctttc ggacaacaga aacaggagat gccagaagga tctcctgttt cagatacata
     2041 ctcgcaaact gccagattgc aatccagaaa agtactgcta atatgcgaat tcctgtatat
     2101 ttcgttccat tgtcagtttt tgctctatcg tttgtaata
//
LOCUS       DBMDXG010000383         2104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10952_length_2104_cov_1.226660, whole genome shotgun sequence.
ACCESSION   DBMDXG010000383 DBMDXG010000000
VERSION     DBMDXG010000383.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10952_length_2104_cov_1.226660"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            347..1717
                     /locus_tag="ACZPD3_09375"
     CDS             347..1717
                     /locus_tag="ACZPD3_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR1416232.1"
                     /translation="MKYDVVIIGAGPGGIFSAYELMKQNNDLKIAVFEAGNPLSKRHC
                     PIDGDKVKTCIKCKTCAIMSGFGGAGAFSDGKYNITNDFGGTLYEHIGKKDALDLMRY
                     VDEINVAYGGQDTKMYSTAGTKFKKLCMQNKLKLLDASVRHLGTDINYVVLENLYAKL
                     KEHVDFHFNTPVERLEVLEDRYRIITKNDTTDCSKCIVSVGRSGSKWMEQICKELDIP
                     TKSNRVDLGVRVELPAVIFSHLTDELYESNIVYRTEKFEDNVRTFCMNPYGIVVNENT
                     NGIVTANGHSYEDPSKQTENTNFALLVAKHFSEPFKDSNGYGESIARLSNMLGGGVIV
                     QRFGDLVRGRRSTVARIEEGMVRPTLAATPGDLSLVLPKRILDGIIEMIYALDKIAPG
                     TANDDTLLYGVEVKFYNMEVDIDENLESRYKGLYIIGDGSGVTHSLSHASASGVYVAR
                     QIVENL"
     gene            complement(1818..>2104)
                     /locus_tag="ACZPD3_09380"
     CDS             complement(1818..>2104)
                     /locus_tag="ACZPD3_09380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR1416233.1"
                     /translation="FYYAAEEDEDYKEINGKWLNEEPYGVVHRVASTGIVRGAASFCL
                     DWAYAQTQNLRMDTYSDNIPMQKLLEKCGFQYCGSFERLGMDKWMAYQKI"
BASE COUNT          630 a          439 c          428 g          607 t
ORIGIN      
        1 ttacccctaa cctgatatct tctcatatct ataatatatt ttgcttatca tatcctgcag
       61 aaagtcaggc ggatattcgc aatctccaca ggggacttac ctgattcggt taaaacatta
      121 tttataatgt tatcctataa gtccattcac aaaaacataa gattttttca tttatttatg
      181 atatacttat tactgtctcc ttcgttctca gtaacgcaga cttatctatg gcattctctt
      241 caggcgcata atatcatccg cttcgcaatt tatcagttaa atagtgcgtt caggagactt
      301 gctgcagatt tacaaaatat ttttacctga aaggatcgta gtatatatga aatatgatgt
      361 agtgatcatc ggcgcaggac ctggcggtat tttctctgcc tatgaactga tgaagcaaaa
      421 taacgattta aagattgctg tttttgaggc tggaaatcct ctcagtaaaa gacattgccc
      481 aattgatggt gacaaagtaa aaacctgtat caaatgtaaa acctgtgcca ttatgagcgg
      541 ctttggcggt gccggtgctt tctctgacgg caaatacaat atcacaaatg atttcggcgg
      601 aacactttat gaacatattg gaaagaaaga cgctcttgac ctgatgcgtt acgtagatga
      661 gatcaatgtt gcctacggcg gacaggacac aaaaatgtat tctacagccg gaaccaaatt
      721 caaaaaactt tgtatgcaga acaaattaaa gcttctggat gcttccgtac gtcatctggg
      781 aacagacatt aactatgtgg ttcttgagaa cttatatgca aaattaaaag agcatgttga
      841 ttttcatttc aacactcctg tagaacgtct ggaagtactt gaagacagat accgtatcat
      901 cacaaagaat gataccactg actgcagcaa atgcattgta tccgtaggca gaagcggaag
      961 caaatggatg gaacagatct gtaaagaact tgatatccca accaaatcaa accgggtaga
     1021 cctgggtgta cgtgtagaac ttcctgctgt tatcttctca cacctgacag acgaactgta
     1081 tgagagtaat attgtctaca gaacagaaaa attcgaagat aatgtgagga ctttctgtat
     1141 gaatccttat ggcatcgttg taaacgaaaa tacaaacggc attgtcacag caaatggaca
     1201 cagctatgaa gatccgtcca aacagacaga aaatacaaac ttcgcactgt tggtagccaa
     1261 acatttctcc gagccattta aagacagtaa tggatatggc gaaagcattg cccgtctttc
     1321 caatatgctg ggcggcggag tgatcgtaca gcgtttcggc gatctggtgc gcggaagaag
     1381 gagtacggtt gcacgtatcg aagaaggtat ggtacgacct actctcgcag ctactcccgg
     1441 tgatctgagt cttgttctcc cgaaacgtat ccttgacgga atcattgaaa tgatttatgc
     1501 tctggataaa atcgctcccg ggacagctaa tgacgacacc ctcctctatg gtgtggaggt
     1561 taagttctat aatatggaag ttgatattga tgaaaatctg gaaagcagat acaaaggcct
     1621 gtatatcatt ggagacggaa gcggtgtaac ccattccctg tcccatgctt ctgcaagcgg
     1681 tgtatatgta gcacgccaga ttgttgagaa tctgtaaaag accggatttc ttctgatgga
     1741 tacctccaac tacagcaatc aataacctta aagtccccat ctgtctctta cagttgaaac
     1801 agatagggac ttgtttatta tattttctgg tatgccatcc atttatccat tcccagacgt
     1861 tcaaatgaac cgcagtactg aaatccgcat ttttccagaa gtttctgcat ggggatgtta
     1921 tcgctgtatg tatccatacg gagattctgt gtctgagcat aggcccagtc cagacagaat
     1981 gatgcggctc ctcttactat tcctgtggac gctaccctgt gaactactcc atagggttct
     2041 tcattcagcc attttccatt aatttcttta taatcttcat cttcttccgc cgcataatag
     2101 aaaa
//
LOCUS       DBMDXG010000384         2101 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_10976_length_2101_cov_1.294500, whole genome shotgun sequence.
ACCESSION   DBMDXG010000384 DBMDXG010000000
VERSION     DBMDXG010000384.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10976_length_2101_cov_1.294500"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..1140
                     /locus_tag="ACZPD3_09385"
                     /pseudo
     CDS             209..1140
                     /locus_tag="ACZPD3_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1147..1842
                     /locus_tag="ACZPD3_09390"
     CDS             1147..1842
                     /locus_tag="ACZPD3_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase CobT-related protein"
                     /protein_id="HIR1416234.1"
                     /translation="MEDVRWPAEQLEEHHLEISNRIRNLFWTVSGDYDTEFEPDTEKY
                     VYSKQTVLYEAVKQGAFARYFDQKKLGMYLMKKLHFSAGEDMLLPLQRFRNYEEPRET
                     NERIFQFRAYANNRDGLALKTVGSSLMERPEKNKILIVLSDGKPCDMSIQRPGTRQPK
                     IYDGEKAVKDTAYEVRRARNQGIFVIGIFVGNEEELSVEKRIYGKDFAYIRNISNFSR
                     IVGTFLRRQTDME"
     gene            1859..2053
                     /locus_tag="ACZPD3_09395"
     CDS             1859..2053
                     /locus_tag="ACZPD3_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416235.1"
                     /translation="MGKIEFANCQDVRNQKKIQELDCPHCHEPGGIEAFVKDGILAED
                     GVCDNCGYILPEGTELEEVE"
BASE COUNT          702 a          343 c          507 g          549 t
ORIGIN      
        1 attacctgtt atgtggaaac ctgccgtatc cggcatggaa gaagcacata tctgtgcaaa
       61 atctaagtaa caggcgtatc cgccgatatt tggtctgccg cttaaagcgg ccggaagcac
      121 gtgactttag tcatgtgagg ttcacgattg agtaaacaga aatattatct gatttgatat
      181 aaccaattgc aaagaaggga aaatcttcat gagaagtcat tggacagaaa tagaaaaata
      241 tttagaagac cagaaggtag cgcaggaact gattggagaa ctgagagatt ttcacatgcg
      301 ttatgaggta gaggatcagg taaaggaacg agtagaaaaa ccggatatcc tattttatgg
      361 gaaaaagatt ctggaaatgt caacagcagc gcttcttcag ggagataatc tgttactttc
      421 cggtgcaaaa gctacaggaa aaatgtacta tgtgagacat tggcatggat attcggaaga
      481 ccggaatacg atatttcttt tcatgtaaat acagacagtg ccgatctgat tggaacggac
      541 acctttataa ataatgaggt tcgccttcgg aaaggctcca tctaccaatg cgcggaattt
      601 ggaggatttg gaatcctgga cgaaatcaat atggcaaaaa atgatgcggt ttctgtactg
      661 catgccacac tggatcatcg gcgcagaatc aatattccag gatataacag gattttactt
      721 catccggcaa cacgatttat cggcacgatg aattatggtt acgcaggtac cagagagcta
      781 aatgaagcat tggtttcaag gtttatggta atcgatatgc ctgaaatgga tgaggatagt
      841 gttctttttg tattaaggca acattttcca gatggggaaa aaagtgcact gaaagctttt
      901 gcaggactgt ttctggattt gcagatcaaa gcttaccatg gagaaatctc aacaaaatct
      961 cttgacctga gaggattggt ttctgctgta aaagcaacaa gaagcggact ttctccaatt
     1021 gatgcaatcc agatgggaat cataaataaa agttttgatg tatttgaaaa agagatcgta
     1081 gaagatgtgg tttctaccag aatcccatct tcctggaccg gaaaagacat atgggggtaa
     1141 acttgtatgg aggatgtcag atggccggcg gaacaactgg aagaacatca tctggagatc
     1201 agcaatcgta tcagaaatct tttttggaca gtcagtggtg attatgatac ggaatttgaa
     1261 ccggatacag aaaaatatgt ttattcaaaa caaacggttc tgtatgaggc agtgaagcag
     1321 ggtgcctttg cacgatattt tgatcagaag aaacttggca tgtatttgat gaaaaaatta
     1381 catttttctg ctggagaaga tatgcttttg cctcttcaga gatttcggaa ttatgaagaa
     1441 ccaagggaaa caaatgaaag aatctttcaa tttcgtgcat atgccaataa cagagatggg
     1501 cttgcactga aaacagtcgg ttcctctctc atggagcgac cggaaaaaaa taagatttta
     1561 atcgtattaa gtgatggaaa accctgtgat atgagcatac aacgaccagg aacccgccag
     1621 cccaagatct atgacggaga aaaagctgtg aaagatactg catatgaggt acgtcgggcc
     1681 agaaatcagg gaattttcgt aattggtatt tttgtaggga atgaagaaga gctctccgtg
     1741 gaaaagagaa tctatggaaa agactttgcc tatattcgta atatcagcaa tttttcccgt
     1801 atagtaggaa cttttttgcg aagacaaact gatatggaat gaaaatggag gaatcactat
     1861 gggtaaaatt gaatttgcca attgccagga tgtaagaaat cagaaaaaga tacaggaatt
     1921 agattgtcca cactgtcatg aaccgggagg cattgaagcc tttgtgaaag acgggatact
     1981 tgcagaggat ggagtatgtg ataactgcgg atatattctt ccagaaggaa cagaactgga
     2041 ggaagtggag taagacagtt tgaagtaatc ccgaatctca cctataaatc cggtgaacag
     2101 a
//
LOCUS       DBMDXG010000385         2080 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11096_length_2080_cov_1.803436, whole genome shotgun sequence.
ACCESSION   DBMDXG010000385 DBMDXG010000000
VERSION     DBMDXG010000385.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11096_length_2080_cov_1.803436"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            275..709
                     /locus_tag="ACZPD3_09400"
     CDS             275..709
                     /locus_tag="ACZPD3_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR1416236.1"
                     /translation="MQISTKGRYALRLMLDLAVHNTGELVKIKDISARENISEKYLEQ
                     IISSLKKAGYVKSLRGAQGGYMLAREPETYTVGTILRLTEGSMKPVACLEDEPNQCSR
                     AGECVTLRLWKMLDEAIEGVLDKVTLQDLKDWYEEMGNDYVI"
     gene            801..1730
                     /gene="cysK"
                     /locus_tag="ACZPD3_09405"
     CDS             801..1730
                     /gene="cysK"
                     /locus_tag="ACZPD3_09405"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR1416237.1"
                     /translation="MAKIYKNAAELVGNTPLLEVGNLEKELGLEARILVKLEYFNPAG
                     SAKDRIALSMIEDAEERGVLKPGAVIIEPTSGNTGIGLASLAAIKGYRVILTMPETMS
                     VERRNILKAYGAEIVLTDGTKGMNGAIAKANELAKEYENSFIPGQFDNPANPAIHKKT
                     TGPEIWRDTDGQVDVFVAGVGTGGTITGVGEYLKSQNPDVKVVAVEPATSPVLSQGKS
                     GPHKIQGIGAGFVPKALNTEVYDEVFPVENEDAFTVGKLIAKHEGILVGISSGAALYA
                     AIRLAKRPENKGKTIVALLPDSGDRYYSTPLFV"
     gene            1913..>2080
                     /locus_tag="ACZPD3_09410"
     CDS             1913..>2080
                     /locus_tag="ACZPD3_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR1416238.1"
                     /translation="MRKAIIAMSGGVDSSVAALLTKETGDECIGATMKLFHNEDIGVK
                     REKTCCSLDDVE"
BASE COUNT          674 a          341 c          521 g          544 t
ORIGIN      
        1 tctcagacct gtagtaattc ttcagacgga ggatggtgca gtggcagaat gcattcttaa
       61 gaataatgga tttaatgtat ttacttcagc agaagaactg gagaaataat actgtataag
      121 acagtgagag aatcacagga ctggggatgg ttctgtgatt ttttgtatat tcatatagga
      181 aagttgaata tatcttgacc atatgactgg gaatactata taataaaaga aaacaatgat
      241 aatccgacga aatttaatca ggaggttttg aaaaatgcag atttctacaa aaggaagata
      301 tgctctgaga cttatgcttg atcttgcagt tcataataca ggagaactgg tcaagatcaa
      361 agatatttct gcaagggaaa atatttcaga aaaatatctg gaacagatta tttcatcatt
      421 aaagaaggcg ggatatgtaa agagcctgag aggggcacag ggcggttata tgcttgccag
      481 agagccggaa acatatacgg tgggaactat tctgagactg acagaaggca gcatgaagcc
      541 ggtagcctgt ctggaggacg aaccgaatca gtgcagcaga gccggggaat gcgtcacact
      601 ccgtctctgg aagatgcttg atgaagctat tgagggagta ctggataagg ttacattgca
      661 ggatcttaaa gactggtatg aagagatggg aaatgattat gtaatttgat tttccctctt
      721 gacaacagtg gatttataga atattatgta tataacatat aattcatata tacttagtat
      781 gaaataagga ggacgaactc atggcaaaaa tctataaaaa tgcagcggaa ctggttggaa
      841 atactcctct ccttgaggtt ggaaatcttg aaaaagagct tgggctggaa gcgagaatac
      901 ttgtgaagct tgagtatttt aacccggcag gaagtgccaa agaccgtatc gccctgagta
      961 tgatcgaaga cgctgaagag agaggcgttc tgaagcctgg tgcagtgatc atcgagccta
     1021 catcaggtaa caccggaatc ggtcttgctt ctcttgctgc aatcaaagga tacagagtga
     1081 tacttacgat gcctgagaca atgagtgttg agagaaggaa tattctgaaa gcatacggtg
     1141 cagaaatcgt attgacagat ggaacaaaag gtatgaatgg cgcaatcgcc aaagcaaacg
     1201 aacttgcgaa ggaatatgag aacagtttta ttccgggaca gtttgataac cctgcaaatc
     1261 ctgcgatcca caaaaagacc acaggtccgg aaatctggag agacacagac ggacaggtag
     1321 atgtttttgt ggcaggtgtt ggtacaggcg gaacgatcac cggtgttggt gaatatctga
     1381 aatcccagaa tcctgacgtg aaagtagtgg cagtagagcc tgcaacttcc cctgtacttt
     1441 ctcagggaaa gagcggtccg cataagattc agggaatcgg tgcgggattt gttccgaagg
     1501 cattaaatac agaggtttat gatgaggttt ttccggttga gaatgaggat gcttttacag
     1561 ttggcaagct gatcgccaaa catgagggaa ttcttgttgg tatttcttcc ggtgcggctc
     1621 tctatgcggc aatccggctg gccaagagac cggagaataa aggaaaaaca atcgtagctc
     1681 ttcttccgga ttccggtgac cgttactatt ctactccatt atttgtgtaa aataaaaaag
     1741 ccctttttag ggcttttttt attttacata aatgtgttag aatagatatg ctgtacaaat
     1801 gatttttatg taactgttca gtattttgca gaacagctat cataacataa ataaaattat
     1861 cataaacaat aatattccgt gtaatcggat cacagaacag gatggaataa aaatgagaaa
     1921 agcaattatc gcaatgagcg gcggagtgga cagctctgtg gcagctctgc ttacaaagga
     1981 aaccggtgat gaatgtatcg gtgctaccat gaaattattt cacaatgagg acataggagt
     2041 aaagagagag aaaacctgct gttctctgga tgatgtggag
//
LOCUS       DBMDXG010000386         2073 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11139_length_2073_cov_2.154158, whole genome shotgun sequence.
ACCESSION   DBMDXG010000386 DBMDXG010000000
VERSION     DBMDXG010000386.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11139_length_2073_cov_2.154158"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            145..771
                     /locus_tag="ACZPD3_09415"
     CDS             145..771
                     /locus_tag="ACZPD3_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HIR1416239.1"
                     /translation="MEYKNQTENRAFQEMLQAAVKRLQNRSGEEIAAKSGAVFHSSRN
                     VLEIMSFYETIEIQLPEFYINSDIDEWHYLTLLHYLDMADGTEGSQKLITFGNLKDGL
                     IRGTKFDRTAEQKLEKLLQDKDPEKIQKACKNLGAEFTETKADLCAVFPVLPRYPVTL
                     KIWFADEEFSASGKIFLQDHADHYLSVEDAVTVGEILLQKLSEAFSSL"
     gene            complement(693..1340)
                     /locus_tag="ACZPD3_09420"
     CDS             complement(693..1340)
                     /locus_tag="ACZPD3_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR1416240.1"
                     /translation="MSEKAKKQDRRTRYTRQTIKDTFLELLKQKSFTKITVTEVCKNA
                     EINRGTFYLHYYDIYDVLSDIFNDMTQDMLTTVDHLFCLNQKSCSYPFCQKIQMESQY
                     RALFLDDAIAPILLEKIAENTKEGYVTWLMSHSLLTFEQAESVFYFQMNGCLSINRLM
                     LRNHCNDWKQIQTVIDKFIKSGLESFLIHDQREENASDNFCNKISPTVTASSTLR"
     gene            1509..>2073
                     /locus_tag="ACZPD3_09425"
     CDS             1509..>2073
                     /locus_tag="ACZPD3_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1416241.1"
                     /translation="MGHVIAVSGKGGVGKTTLCGLLIQYLCESGKRPVLAVDADANAN
                     LNEVLGVEPEVTLGELREEIERAGIDPRYQIPSGMTKQAYLEMRLSDAIAEEDDYDLM
                     VMGRTQGQGCYCFVNGLVQTQVQKLQSHYPYIVVDNEAGMEHISRGILPMMEVAILVS
                     DCSRRGVQAAGRIAKLMNELNFKPQKTG"
BASE COUNT          658 a          336 c          460 g          619 t
ORIGIN      
        1 aagttctcaa cagttctttt taatgtgttg ataaactgtg cattttttaa tgctttgatg
       61 attgtaaata atataattac aactataatg gagattaaag gaaaagtcaa cacagatact
      121 ataaatgttc acaaaggaga acttatggaa tacaaaaatc aaacagaaaa tcgtgcattt
      181 caggaaatgt tgcaggcagc agtaaaacgg ctgcaaaatc gttccggaga ggagattgct
      241 gcaaaaagcg gggctgtttt tcatagcagc cggaatgtac ttgagataat gagtttttat
      301 gaaacgattg aaattcaact tcctgaattc tatattaact cagacataga tgaatggcat
      361 tatctgacgt tgttgcatta tctggatatg gccgatggca cagaaggctc acagaagctc
      421 attacttttg gtaacctgaa agatggattg atacggggaa ccaagtttga ccggactgca
      481 gagcagaaac tggaaaagct tttgcaggat aaagatcctg agaaaattca gaaagcatgt
      541 aaaaacctgg gtgcagaatt cacagagaca aaggcggatc tgtgtgctgt ttttccagtt
      601 ctgccaagat atccggtaac tttaaagata tggtttgcag atgaagaatt ttctgcatct
      661 ggcaaaatat ttcttcagga tcatgccgat cattatctga gtgtagaaga tgcggtaaca
      721 gttggagaaa ttttgttgca aaaattatcg gaagcatttt cttctctctg atcatgaatc
      781 agaaaacttt ccagcccgga tttgataaat ttgtcgatta ctgtctgaat ctgtttccag
      841 tcattacaat gattgcggag catcagtctg ttgatggaaa ggcagccatt catttgaaaa
      901 taaaaaaccg attctgcctg ttcaaaggtc aggaggctgt gtgacataag ccaggtcaca
      961 tagccttctt ttgtattttc tgctattttt tccagaagaa taggggcaat tgcatcatcc
     1021 agaaacagtg ccctgtattg tgattccatc tgtattttct gacagaacgg ataggaacag
     1081 cttttttgat ttagacagaa taaatgatct acagttgtta acatatcctg agtcatatca
     1141 ttaaaaatat ctgataatac atcatagata tcgtagtaat gaagataaaa agtgccacgg
     1201 ttgatttcag catttttgca gacctctgta actgtaattt tcgtaaaact tttctgcttt
     1261 aacaattcta agaacgtatc ttttatggtt tgtctggtat agcgggtacg gcgatcttgt
     1321 tttttcgctt tttctgacat tatatactcc tgtattattt gggatcatat gattcctcaa
     1381 caatttttct tatatgttga gtaaccatac aaatctcaca gactgattat tgtaatttat
     1441 atagtaacaa ctataatgag gtttaaagaa aaagtcaaca gattgttcag aattttagga
     1501 ggaaaaatat gggacatgta attgcagtat caggaaaagg tggcgtagga aaaacaactc
     1561 tttgtggatt gttgatccag tatttatgtg aaagcggaaa acgtccggtc ctagctgtgg
     1621 atgcagatgc aaatgcaaat ttgaacgaag tgctcggtgt tgagccggag gtcacacttg
     1681 gggagctgag agaagaaatt gagcgggcag gcatagaccc acgatatcag attccctccg
     1741 gtatgacaaa acaggcatat ctggaaatgc gtctttctga tgcgattgca gaagaagacg
     1801 attatgattt gatggtaatg ggacgaaccc agggacaggg ctgctattgt tttgtaaatg
     1861 gccttgtgca gacccaggtt cagaaactgc aaagtcatta tccatatatt gtggttgata
     1921 acgaagcagg tatggaacat atcagcagag gaatcctgcc aatgatggaa gttgcgattc
     1981 ttgtgagtga ctgttccaga agaggtgttc aggcagccgg acgtattgca aagctgatga
     2041 atgaattaaa ctttaaacca cagaaaactg gat
//
LOCUS       DBMDXG010000387         2052 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11269_length_2052_cov_1.582266, whole genome shotgun sequence.
ACCESSION   DBMDXG010000387 DBMDXG010000000
VERSION     DBMDXG010000387.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11269_length_2052_cov_1.582266"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="ACZPD3_09430"
     CDS             <1..1674
                     /locus_tag="ACZPD3_09430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1416242.1"
                     /translation="HNIAMGSEVNGEKKYILVMSDRTSDRKMNQALSEAVHAAETANR
                     AKSIFLSNMSHDIRTPMNAIIGFTTLAASNIDDKKRVQDYLGKILSSSKHLLSLINDI
                     LDMSRIESGKIHLEETEVCLSDVLHDLKTIISGQIHAKQLELYMDAMDVTNEDVYCDK
                     LRLNQILLNLLSNAVKFTPAGGTVSVRLRQYPGKAKGSELYEFRVKDNGIGMSQEFVQ
                     KIFSPFERERTSTVSRTQGTGLGMAITKNIVDMMGGTIEVQTEQDKGTEFIVRLPFRT
                     QPEHHRIEKISELEGLKALVVDDDFNTCDSVTKMLVKVGMRSEWTVSGKEAVLRARQS
                     MELGDAFHAYIIDWRLPDMNGIEVTRQIRSLDDNTPIIILTAYDWSDIETEARTAGVT
                     AFCAKPLFMSDIRETLMAAIGQKQAQAEDKILPAADLDFRGRRILLVEDNELNSEIAV
                     EILKEYGFLVDTAENGAEAVEKIKNSTPGDYDLVLMDVQMPVMNGYEATRQIRALTDP
                     ALSGITILAMTANAFDEDRKKVLECGMDGFLSKPIIIEELIDTLQKNLD"
     gene            1745..1900
                     /locus_tag="ACZPD3_09435"
     CDS             1745..1900
                     /locus_tag="ACZPD3_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416243.1"
                     /translation="MQQKIREKVMESLKYLVSLVNDVLGMNKLQSGDLKDSVEYYGQC
                     HKIQSGG"
BASE COUNT          615 a          367 c          538 g          532 t
ORIGIN      
        1 cataatattg caatgggaag tgaggtaaac ggggaaaaga aatatattct ggttatgtca
       61 gaccgtacct ctgaccggaa aatgaaccag gctctttctg aggcagtcca tgcggcagag
      121 actgcaaata gggcgaagag tatttttctt tccaatatgt cccatgatat ccggacacca
      181 atgaatgcga ttattggatt tacaacattg gctgcaagta atattgatga caaaaaaagg
      241 gttcaggatt atctgggcaa aattctttct tccagtaagc atctgctttc actgatcaat
      301 gatatattgg atatgagccg gattgagagc ggaaagatcc atctggaaga aacagaagtc
      361 tgtctgtcgg atgtgcttca tgatctgaag acgatcatca gtggacagat tcatgcgaag
      421 cagctggagc tttatatgga tgccatggat gttacgaatg aggatgtcta ttgtgacaaa
      481 ttgcggctga accagatact gctgaatctt ttgtcaaatg cggttaagtt tactccggca
      541 ggcggaaccg tttctgtccg tctcagacag tacccgggaa aagcaaaagg cagcgaactg
      601 tatgaatttc gggtaaagga taacggaatt ggaatgagtc aggaatttgt acagaagatc
      661 ttttctcctt ttgagcggga gcggacttct acagtcagca ggactcaggg tacgggactt
      721 ggaatggcca ttaccaagaa catcgtggat atgatgggcg gtactattga ggttcagaca
      781 gagcaggaca aaggcaccga gtttatagtc cgtctgccat ttcggactca gcctgagcat
      841 catcgcatag agaagatttc ggaactagag ggtcttaagg cattggttgt agacgatgac
      901 tttaacacct gtgacagtgt aacaaagatg cttgtaaagg ttggaatgcg ttcggaatgg
      961 acagtctcgg gtaaagaggc tgttctccgt gcacgacagt caatggagct gggggatgca
     1021 tttcatgctt atatcatcga ttggcgtctg ccggatatga atggaattga agttacacgt
     1081 cagatccgca gtcttgatga caatacgccg atcattattc tgactgcata cgactggtca
     1141 gatatcgaaa cagaagccag aactgcagga gtgactgcat tctgcgcgaa acctctgttt
     1201 atgtcagata tcagagagac tctgatggct gccatcggtc agaaacaggc ccaggcagag
     1261 gataagattc ttccggcagc agatttagat ttcagaggca ggcgtatact tcttgtggaa
     1321 gataatgaac tgaacagtga aattgcagtg gaaattttga aagaatatgg attcctggtt
     1381 gatacagcag aaaatggagc agaggcagtg gagaaaatta aaaattcaac accaggggat
     1441 tatgatctgg tactgatgga tgtgcagatg cctgtaatga atggatatga ggctaccagg
     1501 cagatccgtg cgcttactga tccggcactg tcgggaatta ccattcttgc catgactgct
     1561 aatgcttttg atgaggatag aaagaaagtt ctggaatgcg gtatggatgg attcttatcc
     1621 aagccaataa ttattgaaga attgatcgat acgttacaaa agaatctgga ttaggtgcct
     1681 ggaacatcat aaaacccaca aggtagaaga gggaaatgga aatcagtacg cagatgatcc
     1741 acagatgcag cagaagatca gagaaaaggt aatggaatcc ctgaaatatc tggtgtctct
     1801 ggtaaatgat gtgctgggta tgaataaact acagagcgga gatttgaagg attctgtcga
     1861 atattatgga caatgccata aaattcagtc aggcgggtag tgtgctcatg gtcggggtta
     1921 aggaagagtt tctgccttta actgtcacaa gatcaaagtt tttatgtgtc acggataatt
     1981 cggcagccaa ttcgctgagc ttctttttca ttcctgacgg gtacagcaaa cgatttgttt
     2041 tcaaagcacc aa
//
LOCUS       DBMDXG010000388         2052 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11273_length_2052_cov_1.277294, whole genome shotgun sequence.
ACCESSION   DBMDXG010000388 DBMDXG010000000
VERSION     DBMDXG010000388.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11273_length_2052_cov_1.277294"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            54..1391
                     /locus_tag="ACZPD3_09440"
     CDS             54..1391
                     /locus_tag="ACZPD3_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR1416244.1"
                     /translation="MPEKMCKKKHLTSFQLIILGFAGVILLGSILLMLPVSSLDKVPT
                     PFHEALFTATSAVCVTGLVVKDSGSYWSVFGQTVILALIQIGGLGVVTVAAAVSLLSG
                     KKISLMQRSTMQDAISAPKVGGIVRLTRFILKGTLLIEAAGAMLLLPVFVSDYGLKGI
                     WMAAFHSVSAFCNAGFDILGTADNAFPSLTGYSGNILINVVIMLLIITGGIGFLTWDD
                     IYRNKMNFKRYRMQSKIILMTTVCLIVFPAFFFFACDLKNLPAGERLLAAMFQSVTTR
                     TAGFNTIDISEMSEASKAVMILLMLIGGSPGSTAGGMKTTTFTVLILNAIATFRSQEN
                     AGAFGRRLEYHVIKNAATIAMLYFTLFFCGGVAISVYEGLPLLDCLYEAASAVGTVGL
                     TLGVTPGLHVFSQVVLIILMYLGRVGGLTLIYAVFSGRNKGNAKLPLEKITVG"
     gene            1411..>2052
                     /locus_tag="ACZPD3_09445"
     CDS             1411..>2052
                     /locus_tag="ACZPD3_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR1416245.1"
                     /translation="MKNVLIIGLGRFGRHIAMQLNQLGHEIMAVDWNEERVDKVLPFV
                     TNAQIGDSTNAEFLQSLGIGNYDICFVTIGGSFQNSLETTSLLKELGAKLVISRAERD
                     VQEKFLLRNGADKVVYPEKQVAKWASIRYTDDHILDYMEVDASHAIFEVEVPEEWTGK
                     TVGGLDIRKRYNINILAVKNEGEFNIAISPDTYLEENSKLLVLGEYRALQKCFR"
BASE COUNT          579 a          352 c          506 g          615 t
ORIGIN      
        1 acgcattatg aagatattgc tatagaatca ttagtaagga gttatgatca tttatgccgg
       61 aaaaaatgtg taagaaaaaa cacctgacgt cttttcagct aattattctg ggatttgcag
      121 gtgtgattct tttgggaagc attttgctga tgcttccggt ttcctctttg gacaaagtgc
      181 cgacgccatt tcatgaggca ctttttacag ccacttcggc agtatgcgtt acaggacttg
      241 tagtaaagga cagcggaagt tactggtctg tgttcggaca gacagttatt cttgcgctta
      301 tacaaatagg tggacttgga gtagtaaccg tagcggcagc ggtttcgctt ctttcaggga
      361 aaaaaatatc tctcatgcaa agaagtacaa tgcaggatgc aatttcagca cctaaagttg
      421 gaggaattgt cagactgaca aggttcattt taaaggggac tcttttgatt gaagcagcag
      481 gagctatgtt attgctgccg gtttttgtat cggattatgg cttaaaggga atctggatgg
      541 cagcctttca ttctgtttct gctttttgta atgcaggatt tgacattctg ggaacagcag
      601 ataatgcctt tccgtctctg acgggctatt ccggaaatat ccttataaat gtggtaatta
      661 tgctgctgat cattacagga ggaattggtt ttcttacctg ggatgatatt tataggaata
      721 aaatgaattt taaacgttac cgcatgcaaa gcaagattat tctcatgact actgtatgcc
      781 tgattgtttt tccggcattt tttttctttg cctgtgattt gaaaaacctg cctgcgggag
      841 aacgcctgtt agctgccatg tttcaatcgg taactacaag aactgccggc tttaatacca
      901 tagacatttc agagatgagc gaggcatcaa aagcggttat gatactgtta atgttgatcg
      961 gtggttcgcc gggttctaca gcaggaggaa tgaaaacaac tacttttacg gtactgattc
     1021 tgaatgcaat tgcaaccttt cgcagtcagg aaaatgcagg ggcttttgga cggagactgg
     1081 agtatcatgt gataaaaaat gcagccacaa tagctatgct ctattttaca ttattctttt
     1141 gtggaggtgt tgcaatcagc gtatacgaag gtcttcccct tttggattgt ctgtatgaag
     1201 cagcttcagc tgtgggcacg gtaggtctga ccttgggagt cacaccggga cttcatgttt
     1261 tttcacaagt tgtgctgatc atactgatgt atctgggacg cgtaggcggc ctgaccctga
     1321 tttatgcagt attctcagga agaaataagg gaaatgcaaa gcttcctctg gagaaaatca
     1381 cagttggatg agaaggagaa aagtaatatc atgaagaatg tattgattat tggacttgga
     1441 agatttggaa gacatattgc catgcagctg aatcagcttg ggcatgaaat tatggcagtt
     1501 gactggaatg aagaaagggt ggacaaagtg cttccgtttg tgactaacgc ccagatcggg
     1561 gacagtacca atgctgaatt tttacaatcc cttggaattg gaaactacga tatctgtttt
     1621 gtgactattg gaggaagctt tcagaattct cttgagacca cttcccttct gaaagaactg
     1681 ggagcaaaac tggtaatatc cagagctgaa cgtgatgttc aggaaaaatt tctcttacgc
     1741 aatggtgcag ataaggtggt ttatccggaa aagcaggttg caaaatgggc atctatccgt
     1801 tacacagatg atcatatcct tgattatatg gaggtggatg cttcccatgc gatttttgag
     1861 gtagaagtgc cagaggaatg gactggaaaa actgtaggcg gactggatat cagaaagcgt
     1921 tacaatatta atattctggc agtaaaaaat gaaggagaat ttaatattgc aatttctccg
     1981 gatacgtatt tggaagagaa tagtaaatta ctggtgctgg gagaatacag agctcttcag
     2041 aagtgcttcc gc
//
LOCUS       DBMDXG010000389         2048 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11310_length_2048_cov_1.242424, whole genome shotgun sequence.
ACCESSION   DBMDXG010000389 DBMDXG010000000
VERSION     DBMDXG010000389.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11310_length_2048_cov_1.242424"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="ACZPD3_09450"
     CDS             complement(<1..1252)
                     /locus_tag="ACZPD3_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HIR1416246.1"
                     /translation="MDVKNYNRNRKKPVRKKGLRIVAFLLLFIGVVLAVFRYYKFMSK
                     SIYEESVSHLTEVLHQSDQMLRELTNKNLTYLHIWGENLQNISGEDEIRDYIKKAQED
                     AGFLEFFFLSSDGDYKMATGETGYLGLQENIEEDIRQGNDVIANAAVPGKSQLLVFAT
                     PKAHGIYQGFEYDAIAIAYENSDIVDVLDISAFDGNAQSFIIHPDGRVVIDHSSELWG
                     NVYNFFGFLSRHSDMSEKEINVLLEKFKAGRTDAMLLNLDGRNYYLVYEKSDIQDWMF
                     LGLVQADIVNASMNNLQFTTMLLVGAVVLCIAAFFISLIIQKNRKNLRRKDVEIRYRD
                     ELFQKLSMNVDDVFLMLDAQTYQTDYVSPNAEKLLGITVEQIRKDIRVLRKLHPADSE
                     DSQKNHLKEIPVHEQQEWDCEFIHR"
     gene            complement(1258..>2048)
                     /locus_tag="ACZPD3_09455"
     CDS             complement(1258..>2048)
                     /locus_tag="ACZPD3_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR1416247.1"
                     /translation="QEVDMQLTEYLKDIKPVIEENHMYIRIASSDFFSVSKDVVSKMI
                     SGEYDAGQAYQSFNSQLLEEETASEDIVLDSQKSYSNRFHSSGGNAAYSVMANTLRGI
                     YGSDVLIATGNSFTGNVLKAGYTEKMAGDMIMPNGLAAYSSKMSGAELKEIIKNFVEG
                     YEGGFIPFNRGSLPVVSGISVEINETEDGYTLSKVTKDGKTVRDEDTFTVTCLATPQH
                     METYPVDENMIFDGGATSVKDTWTAYVSGGNAVLAEPEDYITLR"
BASE COUNT          590 a          506 c          343 g          609 t
ORIGIN      
        1 tccgatgtat aaattcacaa tcccactcct gctgttcatg aaccggaatt tctttcagat
       61 gattcttctg cgagtcttca gaatctgcag ggtgcagttt tcttaaaaca cgaatatcct
      121 tgcgaatctg ttctactgta atacccagca gtttttccgc attagggctg acgtaatccg
      181 tctgatatgt ttgggcatcc agcatcagga aaacgtcatc cacattcatg gaaagctttt
      241 gaaacagctc atcacggtac cggatctcca catcctttct tctgagattt ttcctgtttt
      301 tctggataat aagactgata aaaaaggcag caatgcaaag tacaacagct ccaacaagaa
      361 gcatcgtggt aaactgcaga ttgttcatac tggcattgac aatatcagcc tggacaagtc
      421 ccagaaacat ccagtcctga atatccgatt tttcatagac cagataataa tttcttccat
      481 ccagattcag caacatcgca tcagtacgtc ccgctttaaa cttctccaaa aggacattga
      541 tctctttttc agacatatca gaatgcctac tcagaaaacc gaagaaatta tacacgttcc
      601 cccataactc ggaagaatga tcaatcacaa cacgaccatc cggatgaata ataaaactct
      661 gggcatttcc atcaaaagca gaaatatcca gtacatctac gatatcagaa ttttcataag
      721 caatggcaat tgcatcgtat tcaaatccct gataaattcc atgcgcctta ggtgtggcaa
      781 aaacaagcag ctgggatttt ccgggaactg ctgcattcgc tatcacatca tttccctgcc
      841 ggatatcctc ttcaatattt tcctgtaatc caagataccc ggtttcacct gtcgccatct
      901 tgtagtcacc gtcagatgat agaaaaaaga attccaaaaa gcctgcatct tcctgcgctt
      961 tctttatata atcacggatc tcgtcttcac cggaaatatt ctgcagattc tcgccccaaa
     1021 tatggaggta agtcaggttc ttatttgtca gttctctcag catctgatca gactgatgaa
     1081 gaacttccgt cagatgagaa acactttcct cgtaaataga tttcgacata aacttataat
     1141 atcggaaaac agccagaaca actcctataa agagaagaag aaaagcaact attcttaatc
     1201 cttttttcct gacaggtttc tttctatttc tattataatt cttaacatcc atttttctca
     1261 cctcagggtt atgtaatctt caggttctgc aagaacggca ttaccacctg aaacatatgc
     1321 tgtccaggta tctttcacag atgtagctcc tccatcaaat atcatatttt catctacagg
     1381 ataagtttcc atatgctgcg gtgtggcaag acaggtcacc gtgaaagtat cctcatcccg
     1441 aactgtcttt ccatcctttg taactttact taatgtatag ccatcttccg tttcattaat
     1501 ttccacagaa ataccactaa ccactggcaa agagccacgg ttaaacggaa taaatcctcc
     1561 ctcatagcct tctacaaagt ttttgattat ttctttcagc tcggccccac tcatcttgct
     1621 gctgtaagcc gccagaccat ttggcatgat catgtcacct gccatttttt ctgtgtaacc
     1681 ggctttcagc acatttcccg taaagctgtt tccggttgcg atcagcacat ctgacccata
     1741 gatgccacgt agcgtgtttg ccataacaga atatgctgca tttcctccac tgctgtggaa
     1801 acggttggaa tacgacttct gtgaatccag cacgatatcc tcagaagcag tttcttcctc
     1861 aagaagctga gaattaaatg actggtatgc ctgccctgca tcgtattctc ctgagatcat
     1921 tttggaaacc acatccttgg aaacagagaa aaagtcactt gacgcaatac ggatatacat
     1981 atggttttct tcgatcacag gttttatatc tttcagatat tccgtaagct gcatatccac
     2041 ctcctgcc
//
LOCUS       DBMDXG010000390         1995 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11637_length_1995_cov_1.567054, whole genome shotgun sequence.
ACCESSION   DBMDXG010000390 DBMDXG010000000
VERSION     DBMDXG010000390.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11637_length_1995_cov_1.567054"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="ACZPD3_09460"
     CDS             complement(<1..1440)
                     /locus_tag="ACZPD3_09460"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR1416248.1"
                     /translation="MTEENGVEKVKKRKNFFRSLRFRILIILIILGIVPGVIVTYTML
                     HNYQNRAVAMLTETVGDQCDILCNLIIRENYLNDTDSEVVNSKLELFSNVYNGRILLA
                     DRDFKIVSDTFHTEEGKTLLSSLAVACLKGEETSNYDARSKVLELAVPVQSPDVQQLQ
                     GVMLVSVSAVDIAETLAEMEQRGVMLIGSIVVLSVFLAWLLSTILVKPLARVTKAIED
                     LTDGMLDEEISVPDYTETELITDAFNKMVNRMKILDESRQEFVSNVSHELKTPLTSMK
                     VLADSLVGQQGVPEELYQEFMSDITAEIDRENKIITDLLSLVKMDKKAADVNITHMDI
                     NQLLEDILKRLRPIADRRNIDLILDCFRPVDADVDEVKFTLAVSNLVENGIKYNVDDG
                     WVRVSLDADHKYFYITVADSGMGIPEDSIERIFERFYRVDKSHSKEIGGTGLGLAITK
                     SSVAMHHGTIKVFSKEGEGTTFSVRIPLSY"
     gene            complement(1721..>1995)
                     /locus_tag="ACZPD3_09465"
     CDS             complement(1721..>1995)
                     /locus_tag="ACZPD3_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416249.1"
                     /translation="QCQNEKESMIEMEFQDLKYLKLFPTDPHYTCEILDSTILLKDDS
                     IIWCDCANGKDIKAENDAKVLICAAKLRWRSISGCMGNKEFFKSIK"
BASE COUNT          546 a          487 c          343 g          619 t
ORIGIN      
        1 ataagacaga ggaatacgca cagagaatgt agttccctct ccttctttac tgaatacttt
       61 gatcgttcca tggtgcattg caacagaact cttggtgatc gccagtccca gaccggttcc
      121 cccgatttcc ttggaatggg acttgtccac acgatagaaa cgctcaaaga ttctttctat
      181 actgtcctca gggattccca taccggaatc cgccactgtt atatagaaat atttatggtc
      241 tgcatccagt gacacgcgca cccatccatc atccacatta tatttaatgc cattctccac
      301 cagattgctg acagcaagag taaacttcac ctcatccaca tctgcatcca caggtcggaa
      361 gcaatccagg atcagatcga tattgcgcct gtctgcaatg ggacgcaagc gcttgaggat
      421 atcctccaga agctgattga tatccatatg ggtgatgttt acatctgctg ctttcttatc
      481 catctttacc agcgagagca gatctgtaat gatcttattc tctctgtcga tctcagcagt
      541 aatatcactc ataaactcct gatacagttc ctccgggacg ccctgctgtc caaccagaga
      601 atctgccaga accttcatgg aagtcagggg tgtcttcagt tcatgggaca cattggatac
      661 aaactcctgc ctggattcat caagaatttt catgcggttg accatcttgt tgaatgcatc
      721 tgtgatcagc tctgtctccg tatagtcagg aacactgatt tcttcatcca gcatgccatc
      781 tgtcaggtct tcaatggcct tcgtcactct tgcaagtggt tttacaagta ttgtagagag
      841 cagccatgcc agaaatactg aaagaacaac aatacttccg atcagcatca cgcctctctg
      901 ttccatctct gccagtgttt ctgcaatatc aacagccgaa acacttacca gcatcacgcc
      961 ctgaagctgc tgcacatccg gactctgtac aggaactgca agctccagta ctttacttct
     1021 ggcgtcataa ttgcttgtct cttctccttt cagacaggct acagccagtg aactgagaag
     1081 cgtctttccc tcctctgtgt ggaaggtatc acttactatc ttaaaatctc tgtcagccag
     1141 caggatacgt ccgttataca cattggaaaa cagttccagc ttgctgttta caacctctga
     1201 atctgtatca ttgagataat tctccctgat gatcagatta cataaaatat cgcactgatc
     1261 acccactgtc tctgtaagca ttgcaaccgc ccggttctga tagttatgaa gcatcgtata
     1321 tgttacaata acccccggca cgattcccag aatgatcagg atgatcagaa tacgaaaacg
     1381 cagacttctg aaaaaatttt ttcttttttt caccttttca actccgttct cttctgtcat
     1441 gcagtttgat gcatgctttc tgtctgttcg taaaacagtc attgtttatc gaatccttat
     1501 catcttatca cagaaaaatg ggcttttcta catcttagcg aaaagtaatt tattttttgc
     1561 agcaatcaat gcgcaagaca tatctcagga aatattacct caatcactct tttattatat
     1621 acgctcactt ttgcttctcc gaacagatat gacacaatgg gtacaaaaag aacggtcggt
     1681 ggggaatgcc aaccgttctc tattctacta attttatatt ttatttgata gatttgaaaa
     1741 attctttatt tcccatgcac cctgaaatag aacgccatcg aagtttcgct gcacatataa
     1801 gaactttcgc atcattttcc gcttttatgt cttttccgtt tgcacagtca caccagataa
     1861 tactatcatc ttttaataat attgtagaat ccaaaatttc acatgtataa tgaggatctg
     1921 ttggaaataa tttcaaatat tttaaatctt gaaattccat ttcaatcata gattcctttt
     1981 cattttgaca ttgaa
//
LOCUS       DBMDXG010000391         1993 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11649_length_1993_cov_1.796512, whole genome shotgun sequence.
ACCESSION   DBMDXG010000391 DBMDXG010000000
VERSION     DBMDXG010000391.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11649_length_1993_cov_1.796512"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(165..1367)
                     /locus_tag="ACZPD3_09470"
     CDS             complement(165..1367)
                     /locus_tag="ACZPD3_09470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR1416250.1"
                     /translation="MGMAVVTLKKGEGRLLKSGGMWIFDNEIDTVMGNFENGDIVLVH
                     DFDGYPMGRGFINTNSKITVRLMTRDENVDINEELLEKRVRDAWEYRKKVVDTGCCRL
                     IFAEADFLPGIVVDKFSDVLVVQSLALGIDRFKETIVELLRKVLAEDGITIRGVYERS
                     DVKVRKQEGMEMVKGFIGPEFPTLVQIEENGVKYEVDVKDGQKTGFFLDQKYNRLAIQ
                     KLCKGAKVLDCFTHTGSFALNAGIAGAESVTGVDASQLAVDQATANAALNGLSDSVKF
                     ICEDVFELLPELEEKGEKFDVVILDPPAFTKSRNSIKNAVKGYREINLRAMKLVKDGG
                     FLATCSCSHFMDYELFTKTIGQAAKNVHKRLRQVEYRTQAPDHPILWAADESYYLKFY
                     IFQVCNDR"
     gene            complement(1772..>1993)
                     /locus_tag="ACZPD3_09475"
     CDS             complement(1772..>1993)
                     /locus_tag="ACZPD3_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR1416251.1"
                     /translation="LEEAERLLDTDQYYYLMCTIHPDNQFSRHNMESHGYEVKRTALC
                     YGGLPRCILLKELTESGKSPAQVVKSSKQ"
BASE COUNT          542 a          501 c          310 g          640 t
ORIGIN      
        1 attcttactt gttttggttc aaggttacga acgagcaccg cgagtttcgc aattacctat
       61 ttattctcaa acatccggaa tcctctgctc tgctacagaa atccctgttt tctcaaaaat
      121 cttatcgtgc aagtacactc tggctctgat atcatattat ataattatct gtcattgcat
      181 acctggaaga tatagaactt cagataataa gactcatctg ccgcccacag gattggatga
      241 tccggtgcct gtgtcctgta ttctacctgg cgaagtctct tatgtacatt ctttgccgcc
      301 tgtccgatgg ttttagtaaa taattcataa tccataaaat gagaacagga acaggttgca
      361 agaaaacctc cgtcttttac cagtttcatg gcacgcagat taatttccct gtatcctttt
      421 actgcattct taatagaatt tctggatttg gtgaatgccg gaggatcaag aatcacaaca
      481 tcaaatttct cacccttttc ttccagttct ggaagcagtt caaaaacatc ttcacagata
      541 aatttcacac tgtcagataa accattcaat gccgcattgg ctgttgcctg atctaccgcc
      601 aactgagatg catccacacc ggttacactt tcagcacctg caattccggc attgagtgca
      661 aaggaacctg tatgtgtaaa gcagtccaga actttcgcac ctttgcaaag cttctgaatt
      721 gccagtctgt tatacttctg atccaggaaa aatcctgtct tctgcccgtc ctttacgtca
      781 acttcatatt ttacaccatt ttcttcaatc tgcacaagtg ttggaaactc tggtcctata
      841 aaacccttaa ccatttccat tccttcctgt ttgcgcacct tcacatcgct tctttcgtaa
      901 acaccgcgaa tagtgatccc atcttctgcc aggactttcc tgaggagttc tacgattgtt
      961 tctttaaaac gatcgatccc cagagccagt gactgaacta ccagtacatc cgaaaattta
     1021 tccaccacaa ttcccggaag gaaatctgcc tctgcaaaaa tcagacgaca gcatcctgta
     1081 tctaccactt tcttacgata ttcccatgca tcacgtacac gcttctccaa aagttcttca
     1141 ttaatatcaa cattttcatc tctggtcata agacgtaccg taatcttaga attagtatta
     1201 atgaaccctc ttcccatagg atatccgtca aaatcatgga caagtacaat atcaccgttc
     1261 tcgaaatttc ccatgactgt atctatctca ttatcaaaaa tccacattcc gccggatttc
     1321 agaagtcttc cctctccctt tttcagagtt actactgcca ttcccattcg tatttctcct
     1381 tgtatttttt atgtttatct tttgtcaatt atatgactct gttttctata gcagccctca
     1441 taaataagtt actgttcact ccgttcccag caacacgctt cgcgatgcac agaatttatg
     1501 ctaatcgcat aaattcgcgc agtatgtctc gggttttctg tgaccttcgc tcacaaaaaa
     1561 cacctcgcgg gatattacca gtgaacagta acataaataa tgcatttaag acagtttcag
     1621 atttcacact ctgatgggcc ggattaagat gcattattta cgaggattgc tataatcaca
     1681 tcattttatc acattcatct cattttttcc atacataaca aaaagcagct ggcaaaatcg
     1741 caaaaatttc accaactgct ttccattact attattgttt gctgcttttc acaacctgtg
     1801 caggggactt ccctgattcg gttaactcct taagaaggat acatcttggc agacctccgt
     1861 aacagagtgc cgttctctta acttcatatc catggctttc catattatgt ctgctgaact
     1921 gattatccgg atgtatggtg cacatcagat aataatattg gtctgtatcc agcaatctct
     1981 ccgcttcttc cag
//
LOCUS       DBMDXG010000392         1989 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11671_length_1989_cov_1.242585, whole genome shotgun sequence.
ACCESSION   DBMDXG010000392 DBMDXG010000000
VERSION     DBMDXG010000392.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11671_length_1989_cov_1.242585"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="ACZPD3_09480"
     CDS             complement(<1..832)
                     /locus_tag="ACZPD3_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HIR1416252.1"
                     /translation="MPNIELLEKSLPVAPIRIAALSGCQDLAKEVDKKLVKFRKELVE
                     RKQLSAIPQGYSEESFIVECECPRFGTGEGKGYIKESVRGTDLYIMVDVTNYSESYTV
                     CGHENHMSPDNHFQDLKRIISAATGKAHRINVIMPFLYEGRQHRRTKRESLDCALALK
                     ELSDMGVSNIITFDAHDPRVQNSIPLNGFDNFFPTYQFLKALIKSVPDLRVDNDHLMI
                     ISPDEGAMSRAVYFSNILGVDMGMFYKRRDYSTIVNGKNPIVAHEFLGDSVEGKDVVV
                     I"
     gene            1275..>1989
                     /locus_tag="ACZPD3_09485"
     CDS             1275..>1989
                     /locus_tag="ACZPD3_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR1416253.1"
                     /translation="MKKKKHIISRVLPGSIAQELELEPGDILAAINDTPVEDVFDYRY
                     LMNDEFVTLLIEKADGELWELEVEKEYEEDLGIEFENGLMDEYRSCTNRCIFCFIDQM
                     PPGMRETLYFKDDDSRLSFLQGNYVTLTNMSQKDIDRIIQYRLEPINVSFQTMNPELR
                     CKMLHNRFAGEALLKADQLYEAGITMNGQIVLCKGVNDGKELEFSLEKLSAYAPVLQS
                     VSVVPVGLSKFRQGLYPLEP"
BASE COUNT          553 a          396 c          468 g          572 t
ORIGIN      
        1 cgataactac aacatctttg ccttctacgg aatctccgag gaattcatgt gcaacaatag
       61 gattctttcc attgacgatt gtagagtaat cacgacgttt gtagaacata cccatatcta
      121 ctccaaggat attggagaaa tatacagctc ttgacatggc accttcatca gggctgatga
      181 tcatcagatg gtcattgtca acacgaagat ccggcacact cttgatcaga gcttttaaaa
      241 actgataagt agggaaaaag ttatcaaaac cattcagcgg aatagagttc tggacacgtg
      301 gatcatgggc atcaaaggta atgatattgg aaactcccat atcgcttaat tccttcagtg
      361 ccagtgcaca gtccagagat tctcttttag tacgtctgtg ctggcggcct tcataaagga
      421 acggcataat tacattgata cggtgtgcct tgcctgttgc agcggaaata attcgtttaa
      481 gatcctggaa atggttgtca ggagacatat ggttctcgtg tccgcataca gtataagatt
      541 cactgtaatt ggtcacatca accataatat aaaggtcagt gccacgaacg gattctttaa
      601 tataaccttt accttcaccg gttccgaaac gggggcattc acattctaca ataaatgatt
      661 cttcgctgta tccctgagga atagcactga gctgtttgcg ttcaaccagc tctttacgga
      721 attttacaag tttcttgtca acctcctttg caagatcctg gcaaccggaa agggctgcaa
      781 tacgaatcgg cgcaaccgga agggattttt caagtaactc tatattcggc ataattttcc
      841 tcctgagttt tgtttatatt ttgtttataa aaaataaaac cataatacat ttatatcatt
      901 tgccccaaag cccgtcaagg aatttattgt ttttcgcctg taaaaatcat attgtataca
      961 aaaatgtacg cgattcagtc agagcagaag aatgaacaga tctgtctgcc gtggattcga
     1021 taagagcgta ttttgccagg cgggttccgg gatttggtca gcagaatgcg aaaatgccct
     1081 gtggaatatt gccggtgaag ggctgctcct gctgtttctg tgtaggcggc tgctctttat
     1141 acttgacagc gcaggccggg gtgatataaa atttgcagtt gttttttatg tgtaaaattg
     1201 ttatgatgat tacagcttac agtacggaca gcaggcgaca gggtgtgtct gtacaggcgg
     1261 aagaaaggga ttttatgaaa aaaaagaagc atatcatctc cagggtcctg ccgggaagta
     1321 ttgcccagga actggagctt gagccgggag atatcctggc tgccataaat gacactccgg
     1381 tggaggatgt ttttgattat cgttatctga tgaacgatga atttgtgaca cttctcattg
     1441 aaaaagcaga cggcgagctg tgggaactgg aagtggaaaa agaatatgag gaagatctgg
     1501 ggatcgagtt tgaaaacggg cttatggatg agtaccgctc ctgcaccaac cgctgtattt
     1561 tctgctttat tgatcagatg cctccgggaa tgagagaaac tctttatttt aaggatgacg
     1621 attccagact ttcttttctt cagggaaatt atgtgactct gacaaatatg agccagaagg
     1681 atattgaccg gatcattcag taccgtctgg agcctattaa tgtttcgttt cagacaatga
     1741 atcctgagct tcgctgcaaa atgcttcata accgctttgc aggagaggct cttttaaagg
     1801 cagaccagct ttatgaggcc gggattacca tgaacggtca gatcgttctc tgtaagggcg
     1861 tcaatgatgg aaaagaactg gaattcagtc tggaaaagct ttctgcatat gcgcctgtgc
     1921 ttcagagcgt gtccgtggtt ccggtaggac tttcaaagtt tcgtcagggc ctgtatcctc
     1981 tggagcctt
//
LOCUS       DBMDXG010000393         1980 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11715_length_1980_cov_1.447046, whole genome shotgun sequence.
ACCESSION   DBMDXG010000393 DBMDXG010000000
VERSION     DBMDXG010000393.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11715_length_1980_cov_1.447046"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="ACZPD3_09490"
     CDS             <1..1487
                     /locus_tag="ACZPD3_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR1416254.1"
                     /translation="EVKMLEGQPVISIIGLGDQAVKEAAERIQAAIDESGYVFPKKRV
                     IISLAPSDKKKSGSHFDLAMAVGVLCQNGDIVVKNLKEYGFIGELSLDGRLRACRGIL
                     PMIIAGQKKGIKKIIVPEANLKEAGLVHGVEILGFHDLTGVIRCLEGKTSGGEIQVDE
                     KEKGSEESGTVLDFKDVKGQDELIEAVVLAAAGGHNMLMVGEPGCGKTMIAQRIPTIL
                     PEMTEEECLEVTKIYSISGLLPNGHALMKYRPFRAPHHNASLNALIGGGAYAMPGEVS
                     LAHNGVLFLDELAEFSRRTLDALRQPIEDKKVSISRVNGTHTFPSNFMFITAMNPCPC
                     GYYPGAKCKCTDYEIIKYRGKISGPIMDRIDIQKEVHPVDFFAIADQKSGASSEELRT
                     KVEKARKIQQERYAQEKGINCNAQMTTDLIQRYCVLDKDSLKLLRETSEKYGYSARVI
                     HKLLRLARTSADLDGEEKIRQKDIEKVLTCRELDKSNSKMVVVK"
     gene            1660..>1980
                     /locus_tag="ACZPD3_09495"
     CDS             1660..>1980
                     /locus_tag="ACZPD3_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR1416255.1"
                     /translation="MEKAKRIMDDCQNKNISILCWNDERYPLRAREPADAPPMLYYKG
                     NIKKMDQTVGIVGARRCSQKAKQETVFLASEYAKTRIAVVSGMAKGIDSYAHTACLNA
                     GGYTI"
BASE COUNT          652 a          347 c          530 g          451 t
ORIGIN      
        1 tagaagtaaa gatgctggag ggacagccgg tgatatcgat catcgggctt ggggatcagg
       61 ctgtaaaaga agcggcagag aggatccagg cagcgattga tgagagcggt tatgtttttc
      121 ctaaaaagcg tgtgatcatc agtcttgcac ccagtgataa aaagaaaagc ggttcgcatt
      181 ttgatcttgc aatggcggtt ggagtgctct gccagaacgg agatatagtg gtgaagaacc
      241 tgaaagaata tggctttatc ggagaactgt cattagatgg aaggctaaga gcctgcagag
      301 ggattctgcc gatgatcatt gccggtcaga agaaaggaat aaagaaaatt attgtaccgg
      361 aggctaatct taaagaggca ggactggtgc atggagtaga aattctggga tttcatgatc
      421 tgacaggcgt gatccgctgt cttgaaggaa aaacatcagg gggagaaatc caagtggatg
      481 aaaaggaaaa aggttcggaa gaatcaggga cagttctgga ttttaaagat gttaaaggac
      541 aggatgagct gatagaagcg gttgttctgg ctgcggcagg aggacacaat atgctgatgg
      601 tcggggagcc cggatgcgga aagaccatga tcgcgcagag gattccaacg atacttccgg
      661 aaatgacaga ggaagaatgt ctggaagtaa caaaaatata cagcatttcc ggtctgcttc
      721 ccaatggaca tgccctgatg aaatacagac cgtttcgtgc cccgcatcat aatgcatcat
      781 tgaatgctct gattggcggt ggtgcctatg caatgccggg ggaagtatca ctggcacata
      841 atggggtact gttcctggat gaacttgctg aattttcaag gcggaccctt gacgcattac
      901 gccagccgat agaagataaa aaagtatcta tttcgagagt gaacggaact catacatttc
      961 cttccaattt tatgtttatt acagctatga atccctgtcc gtgtggttat tatccgggtg
     1021 caaaatgtaa gtgtacggat tatgagatca taaaatacag gggaaagatc tctggcccta
     1081 tcatggaccg gatagatata cagaaagaag tccatcctgt agatttcttc gcaatagcag
     1141 accagaaatc aggggcttca tcggaagaat tgcgtacaaa ggtagaaaaa gcccggaaga
     1201 tacagcagga gagatatgcg caggaaaaag ggatcaattg taatgcacag atgacaacgg
     1261 acctcataca gagatattgt gtgctggata aagactcatt gaagctgctg agagagacaa
     1321 gtgaaaagta tggatatagt gcccgtgtga tccacaaact cttaagactt gccaggacca
     1381 gtgcggattt ggatggagaa gaaaaaatcc gtcaaaaaga tatagaaaaa gttctgacgt
     1441 gccgggagct ggacaaaagt aatagtaaaa tggtggtggt taaataatga tatactggat
     1501 atggcttacg cagataccat ttatcgggcc tgttacaacc agatacctga taaaagaact
     1561 gggagatgcg gaaaagattt atcaggctga ctatgaaacc ctaagtgaaa tgtcaggact
     1621 gtctgcaaga caaagagaaa gtacatcagg aatcattcat tggaaaaagc gaagaggatc
     1681 atggatgatt gtcaaaacaa aaatatctct atcttatgct ggaatgatga gcgttatccc
     1741 ttgcgtgcca gagaacctgc tgatgcacca ccaatgctat actataaagg aaacatcaag
     1801 aaaatggatc agacagttgg aatagtaggt gcaagaagat gcagccagaa agcaaaacag
     1861 gaaacggtat ttctggcatc agaatatgca aagacccgga ttgcagttgt cagtggaatg
     1921 gccaaaggaa tcgacagcta tgcacatact gcatgtctca acgccggtgg ctatacaata
//
LOCUS       DBMDXG010000394         1976 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11733_length_1976_cov_1.638933, whole genome shotgun sequence.
ACCESSION   DBMDXG010000394 DBMDXG010000000
VERSION     DBMDXG010000394.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11733_length_1976_cov_1.638933"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="ACZPD3_09500"
     CDS             complement(<1..1298)
                     /locus_tag="ACZPD3_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR1416256.1"
                     /translation="MEIRGIDVSAWQGKIDWKTVADYGMGFAILRITEAGNVIDSYFE
                     QNFSECRKYNIPVGAYKYSYAMTVAEIQSEARKVVEVLNGRKLQYPVWLDLEWNNQRS
                     LGAEQIHKLAEAFEKIITAAGYKFGIYCNVDWYLNVICSHLKKYDFWIARYPASDNGT
                     LQERLRPDFGVGWQYSSKAKIPGISGTVDRNIFYKDYNEAKDIKKENAVMTKSEAINV
                     VLGIAEEEIGYLEKKNNSKLDSKTGNAGSANYTKYWRDIKPSYQGQPWCAAFISWCFM
                     KAFGLDNAKKLLKHWPYVYCPTLGVLFVKNANPKVGDIVIFKHGGTFTHTGFVTKVAG
                     DRFWTIEGNTSGASGIVANGGGVCQKSYYNSNLPGTKFCTPDYSIVLSADKDETDKTT
                     NPEGGSYMFNPETVKAGDKNTSVLLSQEILRARGFKGKNG"
     gene            complement(1343..1636)
                     /locus_tag="ACZPD3_09505"
     CDS             complement(1343..1636)
                     /locus_tag="ACZPD3_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR1416257.1"
                     /translation="MEQITNYVKPELIVVAIALYFVGMALKQAQAVKDKYIPLILGGI
                     SIAICAIYVFATCTCGTGQDIAMAIFTAITQGILIAGLSTYVNQIVKQANKDE"
     gene            complement(1710..1865)
                     /locus_tag="ACZPD3_09510"
     CDS             complement(1710..1865)
                     /locus_tag="ACZPD3_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416258.1"
                     /translation="MSELKLVTRNIRINGIQHKASDMSEEEIKCLLIQRQDIILLNMN
                     YERKAAG"
BASE COUNT          459 a          471 c          357 g          689 t
ORIGIN      
        1 ttgccgtttt tgcctttaaa gcctctggct cttaatattt cctgtgagag aagcacagat
       61 gtatttttgt ctcctgcttt tactgtctct gggttaaaca tgtagctgcc tccttctggg
      121 tttgttgtct tgtctgtttc atctttatct gcagataaaa caattgaata gtccggtgta
      181 cagaattttg tccctggaag gttactgttg taataactct tctggcagac cccgccacca
      241 tttgccacga taccggatgc tccggaagta tttccctcaa tcgtccagaa cctgtctccg
      301 gctacttttg ttacaaagcc ggtatgggta aaggtaccgc catgtttaaa gataacaata
      361 tctccaactt ttggattggc attttttaca aataagacgc ctaaggttgg gcagtataca
      421 tacggccagt gttttaagag tttctttgca ttatccagac caaaagcttt catgaaacac
      481 caagagataa acgctgcgca ccagggctgc ccctgatagg atggttttat atctctccaa
      541 tattttgtat agtttgctga tccggcattt ccagtcttgc tgtcaagctt actgttattt
      601 ttcttttcca ggtacccgat ctcttcttct gcaattccca gaactacgtt gatagcttca
      661 ctctttgtca tgactgcgtt ttcctttttt atatcttttg cttcgttata atctttgtaa
      721 aatatatttc tatctacagt tccgctgatg ccaggtatct ttgctttact ggaatactgc
      781 cagcccacac caaagtccgg ccggagtcgt tcctgtaaag taccgttatc tgatgccgga
      841 taacgtgcaa tccagaaatc gtattttttc agatggctac aaattacatt caggtaccaa
      901 tccacattgc aataaatacc aaatttatat cccgctgccg tgataatctt ttcgaatgct
      961 tctgccaatt tatggatctg ttcagctccg aggcttctct gattattcca ttccagatcc
     1021 agccagaccg gatactgcag ttttcgcccg ttcagaactt ccactacttt tctggcttcg
     1081 ctctgtatct ccgcaactgt catggcataa gaatacttat atgccccaac cgggatattg
     1141 tatttccggc attcagagaa gttctgctca aagtagctat ctatcacgtt tcccgcttct
     1201 gtaatccgca ggattgcgaa ccccatgccg taatcagcaa ctgttttcca gtcaattttc
     1261 ccttgccagg cagatacatc aattcctctt atttccatgt ccgtctcctt tcatagagcg
     1321 aaaagggatg gtttctcatc ccttattcgt ctttatttgc ctgttttaca atctggttca
     1381 cgtatgtaga aagaccagca atcagtattc cctgtgtaat cgctgtaaaa attgccattg
     1441 caatatcctg tccggtaccg caggtgcagg tggcaaacac atagatcgcg cagattgcaa
     1501 tgctgattcc gccaaggata agcgggatgt acttatcctt tactgcctgt gcctgtttga
     1561 gtgccattcc tacgaaatat aaggcaatag ctactacgat gagttccggt tttacatagt
     1621 ttgtgatctg ttccatgatc attctccttt tctttccagg tcttctattc tatgattcgc
     1681 aaccttaata tgttcctcct ttctccgcct taaccggcgg cttttctttc gtaattcata
     1741 ttcagaagaa ttatatcctg tctctggatg agcaggcatt tgatttcttc ttctgacata
     1801 tcactggctt tatgctgaat tccattaata cggatatttc ttgttaccag ttttaattct
     1861 gacatcttcc tcacctcttc tttatggtat gggaaatgat atgtataggt tactgtttat
     1921 acaaatttaa gcagtttgtc gaacgacttt cgttgactct cctctcatat gctctt
//
LOCUS       DBMDXG010000395         1960 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11831_length_1960_cov_2.880581, whole genome shotgun sequence.
ACCESSION   DBMDXG010000395 DBMDXG010000000
VERSION     DBMDXG010000395.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11831_length_1960_cov_2.880581"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(145..1722)
                     /gene="guaA"
                     /locus_tag="ACZPD3_09515"
     CDS             complement(145..1722)
                     /gene="guaA"
                     /locus_tag="ACZPD3_09515"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR1416259.1"
                     /translation="MKRETVVVLDFGGQYNQLVARRVRECNVYCEIYSYKTDLEKIKA
                     MNPKGIILTGGPNSCYEADSPTCNKELFELGIPVLGLCYGAQLMMHVLGGKVEKADVS
                     EYGKTEVLVDKTDSKIFKDVSDKTICWMSHTDYISQVAPGFEIAAHTADCPVATAQNE
                     EKKLYAIQFHPEVLHTVEGKKMLSNFVLGVCGCAGDWKMDAFVEHTIREIREKVGDGK
                     VLLALSGGVDSSVAAGLLSRAIGKQLTCVFVDHGLLRKDEGDEVEGVFGPNGQFDLNF
                     IRVNAQQRYYDKLAGVTEPEAKRKIIGEEFIRIFEEEAKKIGAVDFLAQGTIYPDVVE
                     SGLGGESAVIKSHHNVGGLPDFVDFKEIIEPLRDLFKDEVRKAGLELGIPERLVFRQP
                     FPGPGLGIRIIGEVTAEKVRIVQDADFIYREEVDNAAAEYKKEHGEDPSWMPNQYFAA
                     LTNMRSVGVMGDFRTYDYAVALRAVKTIDFMTAESAEIPYAVLNKVMNRIINEVKGVN
                     RVFYDLTSKPPGTIEFE"
BASE COUNT          499 a          499 c          367 g          595 t
ORIGIN      
        1 aataactgat ttcacgaaat ttgcaggtga atctggaata taaacaagag aaaaaaataa
       61 tttttaaact ccatttgcac tccatttggt cttttcgaca ccccgcaaag cctttattta
      121 tgggcttttc cgcgattttt caagctactc aaactcaatc gtacccggtg gcttgctggt
      181 caggtcatag aatacgcggt taacgccctt aacttcgtta ataatgcggt tcataacctt
      241 attcagtact gcatacggaa tctcagcaga ttcagctgtc atgaagtcga tggtcttcac
      301 tgcgcggagc gctacagcgt aatcataagt tctgaagtca cccataacgc ctacacttcg
      361 catatttgtc agtgctgcaa agtactggtt cggcatccat gaaggatctt caccatgttc
      421 tttcttatac tcagctgcag cattgtctac ctcttcacga taaatgaaat ctgcatcctg
      481 tacaatacga accttctctg cagtaacttc accgataata cggattccaa gtcccggtcc
      541 cgggaatggc tgacggaata ccagtctctc cggaattcca agctccagac ctgctttacg
      601 aacctcatcc ttgaaaaggt cgcggagcgg ttcgatgatt tccttgaaat caacgaaatc
      661 cggaagtccg cctacattgt ggtgagattt gatcactgcg gactctccgc ccagaccact
      721 ctctacaacg tccggataaa tagttccctg tgccaggaaa tctaccgcac caatcttctt
      781 ggcctcttct tcaaaaatac ggataaattc ttcaccgatg atcttacgtt ttgcttcagg
      841 ctctgtaaca cctgccagct tgtcataata tctctgctgt gcattgacgc gaataaagtt
      901 caggtcaaac tgaccgtttg gtccgaatac accctcaacc tcatctccct catctttacg
      961 aagaagacca tgatctacaa atacacaggt aagctgcttg ccgattgctc tggaaagaag
     1021 tcctgctgca acggaagagt caacaccacc tgacagggca aggagaactt ttccatcccc
     1081 gaccttctcg cggatttctc tgatagtgtg ctcaacaaag gcatccattt tccagtctcc
     1141 ggcacatccg catacaccaa gtacgaagtt ggaaagcatc ttctttcctt ctactgtatg
     1201 aagcacttcc ggatggaact ggatcgcata cagcttcttc tcctcattct gtgcagttgc
     1261 taccggacag tctgctgtgt gagcagcaat ctcaaatcct ggtgcaacct gggaaatgta
     1321 atctgtgtgg ctcatccagc agattgtttt atcagatacg tctttgaaga ttttggaatc
     1381 tgtcttatct accagaacct ctgtctttcc atattcgctc acatctgctt tctcaacttt
     1441 tccgcccaga acatgcatca taagctgtgc gccatagcaa agtcccagaa caggaatacc
     1501 aagctcaaat aattctttat tgcatgtagg tgaatctgcc tcatagcagc tgttcggacc
     1561 accggtaagg atgattccct taggattcat tgccttgatt ttctcaagat cggtcttgta
     1621 tgaatagatt tcgcagtata cgttacattc tctcacacgt ctggcaacca gctgattgta
     1681 ctgaccgccg aaatccagta cgacgactgt ttctcttttc atgttttcct ccttgtttct
     1741 cattatttgt aactgtaaag atattgaacc gttaccattg aattttatta taaattacag
     1801 ttcggatttt tacaatgctt attttcagtt ttccaccttg tgttttcttc ctacgttatt
     1861 gttcactccg ttcacagtaa cgtagccaaa attcattcca gattgcctgc ggcaatggaa
     1921 ttttggcttg tatgtctcgg gattttggca tattcatgcc
//
LOCUS       DBMDXG010000396         1943 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_11936_length_1943_cov_1.161238, whole genome shotgun sequence.
ACCESSION   DBMDXG010000396 DBMDXG010000000
VERSION     DBMDXG010000396.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11936_length_1943_cov_1.161238"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(141..761)
                     /locus_tag="ACZPD3_09520"
     CDS             complement(141..761)
                     /locus_tag="ACZPD3_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416260.1"
                     /translation="MKFKKISIGLIALWFLFLTAATFWSVKEENKTLPSVWTEEAQEG
                     SIEYEFPVDLEVSAADQAIGYISMYDNVPESLLQEGRDLLVGKVCSVIRKDDLYELTV
                     DLYEKHSIGENVEGKIEITSEDVFPKVITRQAIHEGDFGKTCVYYIKRQKGAWGYENI
                     LEEKAVTCFPNRNSDFVVLLSEVDEPMVVSTSELTNGERVKLTEKD"
     gene            complement(778..1542)
                     /locus_tag="ACZPD3_09525"
     CDS             complement(778..1542)
                     /locus_tag="ACZPD3_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1416261.1"
                     /translation="MKKRYSNFFEKLICMGAVILAVIPLLFVAGKSVQVPEEYLILLF
                     KRPLYLRMMWNSLIITIPVLIGQLLLAPLAAYGFWQCRWKYKEILFYLYMIVMLMPLQ
                     LTLVPNYLVANWLGIQNSSLAIILPAMFQPLGVFLIRQQIQDFPQETLEAARLDGASE
                     YRIYRSIVKPNLSSAIAAALILIFIDNWNIVDQAVIFLREPYQQPLSVYLCQILEEQP
                     DIFYAASVFYAVPVLLIFLMGQDYLIQGISMSGVKA"
     gene            complement(1564..>1943)
                     /locus_tag="ACZPD3_09530"
     CDS             complement(1564..>1943)
                     /locus_tag="ACZPD3_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR1416262.1"
                     /translation="NISFSIVLFWSGINWIPQIYHEQCQLDGATGRQEFQYITWILLK
                     PTTLVVLLMSIVNSFKVFKEIYMLYGAYPSPYVYMLQHYMNNQFLSLNMQKLSAAAWC
                     MFLILGIFLGIIYRVQRKNLDYL"
BASE COUNT          607 a          471 c          329 g          536 t
ORIGIN      
        1 gggctacaat gcgaaaggta tcatgacgca ttattcggat gacaatgtgt tttaaagcct
       61 gtataaatcg gcacatgcat agaacataat gaaacagcct cacccctgaa agtcccggta
      121 gtgaggctgt attcttttcc ttaatctttt tctgttaatt tcacccgttc tccatttgtc
      181 agttctgatg tgcttacgac catcggttcg tctacttctg ataaaagtac cacgaaatct
      241 gaatttctat ttggaaaaca tgtcacagct ttttcttcca gaatattttc atacccccag
      301 gctcccttct gacgcttaat ataatagaca cacgtttttc caaagtctcc ttcatgaatc
      361 gcctgccgtg tgatcacttt tggaaaaaca tcttcagaag tgatttcgat ttttccctca
      421 acgttttcac caatcgaatg tttttcatac agatctaccg taagctcata caaatcatcc
      481 tttcgaatga cagaacacac ttttccaacc agaagatctc tgccctcctg cagcaaagat
      541 tccggaacgt tatcatacat gctgatatag ccaattgcct gatcagctgc gcttacttcc
      601 aaatcaactg gaaattcata ctcaatgctg ccttcctgtg cctcctctgt ccagacggaa
      661 ggaagcgttt tgttctcttc tttgaccgac caaaacgttg cggcagttaa aaacagaaac
      721 cacagagcga ttaaccctat acttattttt ttgaacttca tcgtactttc ttcccctcta
      781 cgctttcaca ccggacatac tgattccctg tatcaaatag tcctgtccca ttaagaatat
      841 caataaaact ggtactgcgt aaaaaacgga cgctgcataa aagatatccg gctgttcctc
      901 taaaatctgg caaagataca cggataacgg ctgctgatat ggctctctca aaaaaatgac
      961 cgcctggtct acaatattcc aattgtcaat aaaaatcagg attaatgcag cagcaatggc
     1021 tgaactcaaa ttaggcttta caatggaacg ataaattctg tattcgctgg ctccgtccag
     1081 tctggctgct tctaacgttt cctgcggaaa atcctgtatc tgctgacgaa tcagaaatac
     1141 ccccaatggc tgaaacattg ccggaagaat aattgccaag gacgaatttt gaatgcccag
     1201 ccaattcgcc actaaataat ttggcactaa agtcagctgc agcggcataa gcatgacaat
     1261 catgtaaaga tagaacaaaa tctccttgta tttccacctg cattgccaaa agccgtatgc
     1321 ggcaagcggt gcaagcaaaa gctgtccaat caaaaccgga atagtaataa tcagggaatt
     1381 ccacatcatc cgcaaataca aaggtctctt aaagagcaat ataagatact cttctggaac
     1441 ttgtaccgac tttccagcca caaacaacaa cggaatgact gccaaaatga cagctcccat
     1501 gcagatcagc ttttcgaaaa aattcgaata tctctttttc ataaaatcac ccacaaaaga
     1561 tctttacaaa taatccagat tcttcctctg gacccgataa atgatgccca gaaagattcc
     1621 cagtatgaga aacatacacc atgctgcagc agacaatttc tgcatattaa gtgacaaaaa
     1681 ctggttattc atatagtgct gcagcatata aacatacggt gatggatagg ccccatacag
     1741 catatagatc tctttaaaaa ccttaaaaga attcacgatc gacatcaaca gcacaactaa
     1801 cgtcgttggt tttaataaga tccatgtaat atattggaat tcctgccgcc ctgtagctcc
     1861 atccagctgg cactgctcat gatagatctg cggaatccag tttatcccgg accagaaaag
     1921 gacaatcgaa aaactgatat ttt
//
LOCUS       DBMDXG010000397         1926 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12042_length_1926_cov_2.078356, whole genome shotgun sequence.
ACCESSION   DBMDXG010000397 DBMDXG010000000
VERSION     DBMDXG010000397.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12042_length_1926_cov_2.078356"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="ACZPD3_09535"
     CDS             complement(<1..1713)
                     /locus_tag="ACZPD3_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HIR1416263.1"
                     /translation="MEKFQTSDIPKSPRIQKLVDALYEHMPVIESARAKLITESYKET
                     EGEPIITRRAKAFAHILHNIPIIIRDNELIVGSSTIAPRGCQTFPEFSYEWLEAELDT
                     VATRTADPFEIAEETKAELKEADKYWKGKTTSELATSYMAPEAIKAIEHNIFTPGNYF
                     YNGVGHVTVKYWEVLETGFEGIMEKAQKELDGCSVGDGNYARKSHFLEAVILSCKAVI
                     DYAGRYAKLAQEMAAQTSDPVRKQELFVIAENCSRVPAKGAQNFYEACQSFWFVQQLL
                     QMESSGHSISPGRFDQYMYPYYKKDMEAGTITREFAQELMDCIWVKLNDLNKCRDAAS
                     AEGFAGYSLFQNLIAGGQNKEGEDVTNDLSVMCIQASMHVHLPAPSLSVRVWNGSPHE
                     FLIKAAELTRTGIGLPAYYNDEVIIPALQNRGLSLEDAREYNIIGCVEPQKAGKTEGW
                     HDAAFFNMCRPLELVFSNGMDKGEMVGIPTGDVTQMKTFDEFFDAYKKQMEYCISLLV
                     NADNAIDVAHAERCPLPFLSCMIDDCLKEGKSVQEGGAVYNFTGPQGFGIANMADGLF
                     AIRKL"
BASE COUNT          491 a          488 c          348 g          599 t
ORIGIN      
        1 cagtttgcgg attgcaaaaa gtccatctgc catattggcg attccaaatc cctgaggacc
       61 tgtaaagttg taaacagctc caccttcctg gacagacttg ccttctttca gacagtcatc
      121 tatcatacag gaaaggaatg gtaacggaca gcgttccgca tgagctacat caatagcatt
      181 atctgcattc accagcaggg aaatgcagta ttccatctgt ttcttatatg cgtcaaagaa
      241 ttcatcaaag gtcttcatct gagtgacatc gccggtaggg attcccacca tctcaccttt
      301 atccatacca ttcgaaaaaa caagttccag aggtctgcac atattaaaga atgcggcatc
      361 atgccaccct tctgtcttac cggctttctg cggttccaca catccgatga tattgtactc
      421 tctggcatcc tccagggata agcctctgtt ctgcagtgcc ggaataatca cctcatcatt
      481 gtaatatgca ggaagtccga ttcctgttct cgtaagctcg gctgccttta tcaggaattc
      541 atgcggagaa ccattccata ctctcacaga aagtgatgga gccggaagat gcacatgcat
      601 ggatgcctga atacacataa cagaaagatc gttagttaca tcctctccct ctttattctg
      661 accacccgcg atcagattct ggaaaaggct atatcctgca aatccttccg ctgaagccgc
      721 atcacgacat ttattcaggt catttaattt aacccagatg caatccatca attcctgtgc
      781 gaactctcgg gtaatagttc ctgcttccat atccttctta taatatggat acatatactg
      841 atcaaagcgc cccggtgaaa tagaatgtcc actggattcc atctgaagaa gctgctgtac
      901 aaaccagaat gactgacatg cctcatagaa attctgtgca ccctttgccg gtaccctgct
      961 gcagttctct gcgataacaa aaagttcctg ttttcttacc ggatcacttg tctgtgccgc
     1021 catttcctgc gcaagttttg catatctgcc tgcataatcg atcaccgcct tacagcttaa
     1081 aatcactgcc tcaaggaagt gagactttct tgcataatta ccgtcaccga cactgcatcc
     1141 atccagctct ttctgagcct tctccatgat tccctcaaaa ccggtttcca gtacttccca
     1201 gtatttaact gttacatgtc ctacaccatt gtaaaaataa tttccaggtg taaaaatatt
     1261 gtgttcaatt gccttgattg cttccggtgc catataggaa gttgcaagtt cactggttgt
     1321 tttccctttc caatacttat ctgcctcctt aagttcggct ttggtctcct cagcaatctc
     1381 aaaaggatcc gcagttctgg tcgcaactgt atccagttca gcttccagcc actcataaga
     1441 gaactccgga aatgtctgac atcccctcgg cgcaatcgta ctgcttccga caatcagttc
     1501 attgtcacgg ataatgatcg gaatgttatg aagaatatgc gcaaaagcct ttgctctgcg
     1561 tgtgatgatc ggttctcctt ctgtttcctt ataagattct gtgatcagct ttgctctggc
     1621 tgattcgatc accggcatat gttcatacaa tgcatctacc agtttctgaa ttctgggaga
     1681 cttgggaata tctgatgttt gaaatttttc catgttactc ctttctcatg aataaaagct
     1741 gcacccatac cagaccccaa tacattccgg tatcattctg acagcaatat cctccatgtt
     1801 ttcatataga ttcattatac cttatggcta tcgcgaagtc aacatgaatc aacatgtaca
     1861 tcctgaactt ttatgttgtt ttttatttgt tttttcttct gttctggttg tttttgtttt
     1921 gttttt
//
LOCUS       DBMDXG010000398         1923 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12065_length_1923_cov_1.265093, whole genome shotgun sequence.
ACCESSION   DBMDXG010000398 DBMDXG010000000
VERSION     DBMDXG010000398.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12065_length_1923_cov_1.265093"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="ACZPD3_09540"
     CDS             complement(<1..1041)
                     /locus_tag="ACZPD3_09540"
                     /inference="COORDINATES: protein motif:HMM:NF040203.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR1416264.1"
                     /translation="MQNVNRKFKIWGALLFLLFFAVGVSMYFTAANVQAATKTGFVTI
                     NGDSYYINEDGSKQKGWLEVEGKKYYFNTKTGVQVKGWVTDSKGRKRYFSKQAGIMMT
                     GWVTDSKDQKRYFNPSTGFMQTKWLTLKGKRYYFYSNSGVAACKTFLTDSKKNTRYFT
                     SACYMLTGWTKNSSNEYRYFETEDGIMAKGFQTLDGKKYYFNTGSGKMAVGWTTIDGN
                     KYYFDKETGVMATGDVTIDGQKYHFNSNGILSNTTSPTGSRTIKNYLAGALQPVGQAL
                     YVWGGGWNDSTRKGTSQTMTDFYNSQSSSYDYNNYRDLSTANRAKGFDCSGFVGWSVR
                     RNAPESCTDPYSG"
     gene            complement(1393..>1923)
                     /locus_tag="ACZPD3_09545"
     CDS             complement(1393..>1923)
                     /locus_tag="ACZPD3_09545"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HIR1416265.1"
                     /translation="SQGHSDAVRRIKGVKDAKQYTIPVEAALKAVRNGENPDLTTRQK
                     HTRECFVVAEEGADLAQIENDIKTMPNYFSDYDTTVHFISEEELKRDHSGIPHGGFVI
                     RSGKTGWNDENNHVIEYSLKLDSNPEFTSSVLVAYARAAYRMNKEGQSGAKTVFDVAP
                     AYLCAADGAELRKHLL"
BASE COUNT          507 a          457 c          310 g          649 t
ORIGIN      
        1 gccagagtac ggatcagtgc agctctctgg cgcattcctc cggactgacc atcctacgaa
       61 accggaacag tcaaaacctt ttgctctgtt ggctgtgctg agatctctgt agttattgta
      121 atcataactg ctgctctgac tgttatagaa atcagtcatt gtctgacttg ttccttttct
      181 ggtggaatca ttccatccgc caccccatac gtacagagcc tgaccaacag gctgtaatgc
      241 acctgccaga taattcttga ttgttctgga accggtcgga cttgtagtat tgctcaatat
      301 accattggaa ttaaaatggt atttctgtcc atcaattgtt acatctccgg tagccatcac
      361 accggtttcc ttatcaaaat aatacttatt tccatcaatt gtcgtccagc ctacagccat
      421 cttgccgctg cctgtattaa aataatattt ttttccgtcg agtgtctgga aaccttttgc
      481 cataattccg tcttcagttt caaaatatct gtattcattg ctggaattct ttgtccagcc
      541 ggtaagcata taacatgcac ttgtaaaata tctggtattt ttcttactgt ctgtaaggaa
      601 tgtcttgcag gcagccactc cactgttact gtagaaataa tatctctttc ccttcaatgt
      661 cagccatttc gtctgcatga atccggtaga tggattaaaa tatcttttct gatcctttga
      721 atcagtgacc cagcctgtca tcatgatacc agcctgttta ctgaaatatc tctttcgtcc
      781 tttactgtca gtaacccagc cctttacctg aacaccggtc tttgtgttaa agtaatattt
      841 ctttccttca acttccagcc aacccttctg tttagagcca tcttcattta tgtagtatga
      901 atctccgtta atagttacaa aacctgtttt cgttgcagcc tgtacatttg ccgcagtaaa
      961 atacatggaa actcccacag caaaaaacag cagaaacaga agtgcccccc agattttaaa
     1021 cttcctgttt acgttttgca tacatacctt ccttttcttt tttcattatc agagcgtgtc
     1081 tgaaaagtat gaatcgtggg tatcgctctg atatctcaca tacttctcag atacgctcta
     1141 aggtttaaca aagtttttta caatgttatt acgtttctat gactctatta tattacactt
     1201 taaatatccc gtcaacagac aagtaaaatt ctgttaaaca ttcactaatt tttcacagtc
     1261 tgttccataa aaaaagagac aaagtccaca aatactttgt ctcttttcac aagataacac
     1321 tgtgaaatat aacctgtata cctgttttct ctttattttt tcaacagaca gaatattatc
     1381 ttatatccct gatcacaaca gatgctttct taattcagca ccatcagcag cacataaata
     1441 agcaggtgcc acatcaaata cggtcttcgc accgctctgt ccttctttat tcatacgata
     1501 tgcagctctt gcatatgcaa caagtacaga agatgtaaac tccggattgg aatccagctt
     1561 cagactatat tcgatcacat gattgttctc atcattccag cccgtttttc cggatcggat
     1621 cacgaaacca ccatgtggga ttccactgtg atctcgcttc agttcttctt cagaaataaa
     1681 atgtacagtt gtgtcatagt cagagaaata gttaggcata gtcttgatat cattctcaat
     1741 ctgtgccaga tccgcacctt cctcagcaac tacaaagcat tctcttgtat gtttctgtct
     1801 tgttgtaaga tccggatttt ctccgtttct cactgctttc agtgctgctt caacaggaat
     1861 tgtatactgt tttgcatctt ttacgccttt gatacggcgt actgcatctg agtgtccctg
     1921 gct
//
LOCUS       DBMDXG010000399         1921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12072_length_1921_cov_1.748352, whole genome shotgun sequence.
ACCESSION   DBMDXG010000399 DBMDXG010000000
VERSION     DBMDXG010000399.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12072_length_1921_cov_1.748352"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="ACZPD3_09550"
     CDS             <1..414
                     /locus_tag="ACZPD3_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR1416266.1"
                     /translation="YPVINRDLLITASLLHDIGKTKELSAFPLNDYTDEGQLLGHIYM
                     GAQMINDLARQIPEFPEVLKNELIHCILSHHGELEYGSPKKPALVEAVALNLADNTDA
                     RMETITEIFAANKSKKEWLGYNKLFESNLRRTDEV"
     gene            420..1784
                     /gene="rlmD"
                     /locus_tag="ACZPD3_09555"
     CDS             420..1784
                     /gene="rlmD"
                     /locus_tag="ACZPD3_09555"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR1416267.1"
                     /translation="MEFRKNDLVTLEIEDCGIDGEGIGKADGFTVFVKDAVIGDTVTA
                     KIIKAKKNYGYGRLMEVLKPSPYRVEPKCEFARQCGGCQLQALSYDQQLVFKTNKVKG
                     HLERIGGFTDIPMEPIIGMDELFHYRNKAQFPVGRNKEGKIVTGFYAGRTHNIIENRD
                     CALGVAENKEVLDRVIAHMEKYGIEPYNEATGKGLVRHVLIRYGYFTKEVMVCLILNG
                     NKLPKEEQLVKSLCEIPGMTSITINVNKKRSNVILGEEICLLWGQEYITDRIGDISYQ
                     ISPLSFYQVNPMQTQKLYAKALEYADLHGQETVWDLYCGIGTISLFLAQKAKFVRGVE
                     IVPAAIENAKENAKLNGLENTEFFVGKAEEVLPREYKKNGVYADVIVVDPPRKGCDET
                     LLETMVEMNPERIVYVSCDSATLARDLKYLCERGYELRKVCPVDQFGMTVHVESCALL
                     SKLE"
BASE COUNT          594 a          364 c          479 g          484 t
ORIGIN      
        1 tacccggtca tcaacaggga tttactgatc acagcatcac ttcttcatga tatcggaaag
       61 acaaaagaat tatctgcatt tccgttaaat gattatacag atgaaggtca gcttctggga
      121 catatttata tgggagcgca gatgatcaat gatcttgcgc gccagatacc ggaatttccg
      181 gaggtgctta agaatgaact gatccattgt atcctgtccc accatggaga actggaatat
      241 ggttctccga aaaaacctgc attggtggag gccgtagccc tcaatcttgc agataataca
      301 gatgcaagaa tggaaaccat cacagagatt tttgcagcaa acaagagcaa aaaagagtgg
      361 cttggatata ataaattgtt tgaatccaac ttaagaagaa cagatgaggt gtaatcttta
      421 tggaatttcg taagaacgat cttgttacgt tagagataga agattgcgga attgacggtg
      481 aaggtatcgg aaaagccgat ggcttcaccg tttttgtcaa ggacgcagtg atcggtgata
      541 cagtaacagc caaaattatc aaggcaaaga aaaattatgg ttacggccgt cttatggagg
      601 tgctgaagcc atctccgtac agagttgagc ccaagtgcga atttgcccgt cagtgtggcg
      661 gatgccagct tcaggcactt tcctatgacc aacagcttgt atttaagaca aataaggtga
      721 agggacatct ggaacgtatc ggtggcttta cagatatccc catggagccg atcattggca
      781 tggatgaact ttttcattac agaaataagg cacagtttcc tgtaggcaga aataaagagg
      841 gaaaaatcgt tacaggtttc tatgcaggaa gaactcacaa tattatcgaa aaccgtgact
      901 gtgcacttgg tgtagctgag aataaagaag ttctggaccg tgtgatcgca catatggaga
      961 aatacgggat tgaaccatat aatgaagcaa caggcaaagg gctggtgcgc catgtcctga
     1021 tccgttacgg atacttcaca aaagaagtga tggtctgtct gattctgaac ggaaacaagc
     1081 tcccgaaaga ggagcagctt gtaaaatctc tgtgtgagat tccgggaatg acaagcatta
     1141 ccatcaatgt aaacaagaaa cgcagcaatg tgattcttgg cgaagagatc tgtctgctct
     1201 ggggccagga atatatcaca gacaggatcg gagatatttc ctatcagatt tctcctctgt
     1261 ctttctatca ggtaaatcct atgcagaccc agaagcttta tgcaaaggca ctggaatacg
     1321 cagatcttca cggtcaggaa acagtatggg atctctactg tggaatcgga accatttccc
     1381 tgttcctggc acagaaagca aaatttgtcc gcggtgtgga aattgttcct gcagctatag
     1441 agaatgcgaa agaaaatgcg aaacttaatg gcctggagaa tacagagttc tttgtgggta
     1501 aggcggaaga agtgttgcca agagagtata agaaaaatgg cgtttatgca gatgtcatcg
     1561 tcgtagaccc gccaagaaaa ggctgtgatg aaaccctgct ggagaccatg gtcgagatga
     1621 acccggaaag gattgtgtat gtcagctgtg acagtgcgac gttggcaagg gatctgaagt
     1681 atctgtgtga gagaggatat gagcttagaa aagtatgccc ggttgatcaa tttgggatga
     1741 ctgttcatgt ggaaagttgc gctttattgt ctaaactaga ataaaaactg gatgagaagg
     1801 tgactagtac atcgttttcg aaataactat accactcact tgtctgtgaa tccgattgca
     1861 caggtctaaa agcctcagcg tagcccgcta cgcctgcgtt tttataccag cacactcgaa
     1921 t
//
LOCUS       DBMDXG010000400         1889 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12360_length_1889_cov_1.590045, whole genome shotgun sequence.
ACCESSION   DBMDXG010000400 DBMDXG010000000
VERSION     DBMDXG010000400.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12360_length_1889_cov_1.590045"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            155..697
                     /locus_tag="ACZPD3_09560"
     CDS             155..697
                     /locus_tag="ACZPD3_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR1416268.1"
                     /translation="MPLPKERIYTIDDIYALPDGERAELIDGQIYMMAPPNTRHQVIV
                     GELYATIRNYIKSKSGSCKPYVSPFAVFLNEDNKNYVEPDLTVVCSPDKVDEKGCHGA
                     PDWVIEVVSPATQSKDYGIKLFKYRMSGVREYWIINPMKGIVNVYDFENESGTGLYSF
                     DDEILVCIYPDLSIVISELL"
     gene            867..>1889
                     /locus_tag="ACZPD3_09565"
     CDS             867..>1889
                     /locus_tag="ACZPD3_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR1416269.1"
                     /translation="MSSKVACLYIRVSTEDQTELSPDAQKRLLLDYAQKNDMIVSGDF
                     IFTESVSGRHAQKRPEFQKMIALAKQPSHPIDVILVWKFSRFARNQEESIVYKSMLKK
                     DNVDVISVSEPLIGGPFGSLIERIIEWMDEYYSIRLSGEVLRGMKEKALQKGYQTSPC
                     LGYTAVGHGKPFVINESEYAIVSYIMDLYDNQNLDETAIARRCNDLGYRTKRGNLFER
                     RSVDRILGNPFYCGTVVWNGVEFEGSHEVRLSRERYEKRQKLITSRKRPVKARNVSAC
                     KHWLSGLLKCSVCGATLSYTGNNKCPYFQCWKYAKGFHKTSVALSVKKAEEAVISYFD
                     QILDGAE"
BASE COUNT          549 a          375 c          437 g          528 t
ORIGIN      
        1 aaaccgcccc tgctagtaac agggacggat caagaatctc cgaagagatc cagtacttta
       61 gcaaagatat tgtatcatct tcggagcagt tacacaagtc gaacatttgt atatatgtga
      121 tcacatcaat ggattaacga aaggagtttt cattatgcca ttacccaaag aacggattta
      181 tacaatagat gacatctacg ctcttcctga tggcgaacgt gcagagctga ttgatggaca
      241 gatctatatg atggcaccac ctaataccag gcatcaggta atcgtcggtg aactgtatgc
      301 tactatccgc aattacatta aaagtaaaag cggatcctgt aaaccatatg tctctccatt
      361 tgcagtgttc ctgaatgaag ataacaagaa ctatgtcgaa ccagacttaa cagttgtctg
      421 ctcaccggac aaagtagatg aaaaaggttg tcatggtgca cctgactggg taattgaggt
      481 tgtttctcct gctacccaga gtaaagatta cggaataaag ctgtttaaat accgtatgtc
      541 cggagtcagg gaatactgga tcattaatcc tatgaagggg attgtaaacg tttacgattt
      601 tgaaaatgaa tcgggtaccg gattatactc ttttgatgat gaaattctag tatgtatata
      661 tcccgattta tcaattgtga tctctgaatt attataataa aaaccgcccc tgttggtagc
      721 agggacggct caagaatctc cgaagagatt ccgtactttg gcaaagatat tgtatcatct
      781 tcggagcagt tacacaatca gaacatttgt gtggctgtta tttttatact taaaattaca
      841 tattttataa aaccgaggtg atatttatga gcagtaaagt ggcatgtctt tacatccgcg
      901 tctcgacaga ggaccaaaca gaactttctc ctgatgcgca gaaacgtctt ttgctggatt
      961 atgctcagaa gaatgacatg attgtttccg gggactttat ctttactgag agtgtttccg
     1021 gccggcatgc acagaagcgc ccggagtttc agaagatgat tgccctggca aagcagccct
     1081 ctcaccccat tgatgtaatc ctggtatgga aattcagtcg tttcgcccgt aaccaggaag
     1141 agtctatcgt atacaagagt atgctcaaaa aagacaatgt agacgtgatc agtgtatctg
     1201 agccactgat tgggggacct tttggcagcc tgatcgagcg tatcatcgaa tggatggatg
     1261 aatactactc cattcgattg tcaggtgagg ttttgcgtgg catgaaagaa aaagccctgc
     1321 aaaaaggcta tcagacatct ccctgtcttg gctatactgc agttggacat gggaaacctt
     1381 ttgtgattaa tgaatcagaa tatgctattg tctcttatat catggatttg tatgataacc
     1441 agaacttaga tgaaacagct attgccagac gctgcaatga ccttggatac cggacaaaac
     1501 ggggaaacct cttcgagcgg cgcagcgttg accggattct tggaaatccc ttctattgcg
     1561 ggactgttgt ctggaacgga gtggaatttg aaggaagcca tgaggtacgt ctttccaggg
     1621 aacggtatga aaaacgtcag aagctgatca cttcccggaa acgtccagtc aaggcgcgga
     1681 atgtctctgc ctgtaagcac tggctatccg gtcttttgaa gtgctctgtc tgcggggcca
     1741 cgctttctta caccggtaat aataagtgtc cttatttcca gtgctggaag tacgcaaagg
     1801 gatttcataa gacttctgtt gccttatcgg tcaaaaaggc tgaagaagct gtgataagtt
     1861 attttgatca gatcttagat ggagcagaa
//
LOCUS       DBMDXG010000401         1889 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12361_length_1889_cov_1.404362, whole genome shotgun sequence.
ACCESSION   DBMDXG010000401 DBMDXG010000000
VERSION     DBMDXG010000401.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12361_length_1889_cov_1.404362"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(119..337)
                     /gene="infA"
                     /locus_tag="ACZPD3_09570"
     CDS             complement(119..337)
                     /gene="infA"
                     /locus_tag="ACZPD3_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR1416270.1"
                     /translation="MSKADVIEVEGTVLEKLPNAMFKVELENKHVVLAHISGKLRMNF
                     IRILPGDKVTIELSPYDLSKGRIIWRDK"
     gene            complement(363..1118)
                     /gene="map"
                     /locus_tag="ACZPD3_09575"
     CDS             complement(363..1118)
                     /gene="map"
                     /locus_tag="ACZPD3_09575"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR1416271.1"
                     /translation="MSVSIKTEHEIELMREAGHLLEKVHDGLIPYIKPGVSTKEIDRI
                     GEQMIRDLGCIPNFLNYGGFPASFCISLNDEVVHGIPSEEKIIQEGDLVKIDAGLIYK
                     GYHSDAARTYAVGEVSPQARKLMDVTRECFFEGLKAARAGNHLNDISKAIGAHAAKYH
                     YGIVRDLVGHGIGTHLHEDPQIPNFPQKRRGVRLMPGMTLAVEPMINLGRADVAWLDD
                     EWTVVTMDGSLSAHYENTILITDGDPEILTLTK"
     gene            complement(1151..1795)
                     /locus_tag="ACZPD3_09580"
     CDS             complement(1151..1795)
                     /locus_tag="ACZPD3_09580"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR1416272.1"
                     /translation="MKIIMLGAPGAGKGTQAKKIAAKYQIPHISTGDIFRANIKNGTE
                     LGKKAKTYMDQGLLVPDELTCDLVVDRIQQPDAANGYVLDGFPRTIPQAECLTEALNK
                     LGSKVDYAIDVDVPDSNIVNRMSGRRACLKCGATYHVVHAAPKVEGVCDTCGEKLVLR
                     DDDQPETVQKRLNVYHEQTQPLIDYYTKEGILKSVDGTKDLEEVFADIVAILGE"
BASE COUNT          511 a          504 c          319 g          555 t
ORIGIN      
        1 gaaaccgcac atgaaatgaa ttatttttct acgcgtcccc ctttacagca caaaaaaaga
       61 gaaccagatc cctccagctc tctttctcat tactgcacca actgcagtcc aatatttctt
      121 acttatctct ccagataatc ctgcccttag acagatcata cggagacagt tcaatcgtaa
      181 ctttatctcc gggaagaata cgaataaaat tcatacgtaa cttaccactg atatgtgcaa
      241 gcaccacatg tttgttctcc agttccactt taaacatcgc attaggcagc ttttccagaa
      301 cagtgccttc cacttcgatt acatctgctt ttgacatttt aaacctcctg tatgtcgtcc
      361 aattatttcg taagagtcag aatctccgga tctccgtctg taataagaat cgtattctca
      421 taatgagcag aaagagaacc atccatagtt acaacagtcc actcatcatc cagccatgcc
      481 acatctgcac gtcccagatt gatcataggc tctacagcaa gtgtcatacc cggcatcaga
      541 cgaactcctc ttcgcttctg cggaaaattc gggatctgcg gatcctcatg aaggtgagtg
      601 ccgattccat gtccaaccag gtcgcggaca atcccataat gatatttggc agcatgagca
      661 ccgattgcct tggaaatatc attcagatga tttcccgcac gtgcggcctt gagtccctca
      721 aagaaacact ctctggtcac atccatcagc ttacgtgcct gcggtgaaac ttcaccgaca
      781 gcataagtcc tcgctgcatc agaatgatac cctttataaa taagccctgc atcaatcttt
      841 acaagatctc cctcctgaat gatcttctct tcagacggga ttccatgaac tacctcatca
      901 ttcagactga tacagaatga cgccggaaac cctccgtagt tcaggaaatt cgggatgcat
      961 cccagatcac gtatcatctg ttctccgata cgatcaatct cctttgtgct cactcccggt
     1021 ttaatgtaag ggatcagacc atcatgcact ttttcaagca gatgacctgc ctccctcata
     1081 agttcaattt catgttctgt cttgatagaa accgacattt tacctccaga tggtgtttaa
     1141 aaacacccaa ttactctccc aggatggcaa caatatctgc aaatacttct tcaagatctt
     1201 ttgtaccatc cactgatttt aaaattccct ctttagtata ataatcgatc agcggctgtg
     1261 tctgctcatg atatacgtta agacgtttct gaactgtttc aggctggtca tcatcacgaa
     1321 gcaccagttt ctctccacag gtatcacaaa cgccctcaac cttcggtgct gcatgtacaa
     1381 catgataagt agcaccacat ttcaggcagg cacgtcttcc ggacatacgg tttacaatat
     1441 tggaatccgg aacatccaca tcgattgcat aatcaacttt acttcccagt ttgttcagag
     1501 cctctgttaa gcactctgcc tgaggaattg ttcttggaaa accatccagc acataaccat
     1561 ttgctgcatc cggctgttgt attctgtcca caaccagatc acaggtcagt tcatcaggta
     1621 cgagaagtcc ctgatccata taagtttttg cttttttacc cagttctgtt ccatttttaa
     1681 tgtttgcgcg gaaaatatct ccggtagaaa tatgcggaat ctgatatttc gcagcaattt
     1741 tcttcgcctg agttcctttt cctgcccccg gtgcacccaa cataatgatt ttcataagtt
     1801 ataccctccg ttttcttttt tagcaaatca aggctgtgat gccatgcacc acaacctctc
     1861 atttttaatt gttcagaaag cctttgtaa
//
LOCUS       DBMDXG010000402         1866 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12527_length_1866_cov_1.577904, whole genome shotgun sequence.
ACCESSION   DBMDXG010000402 DBMDXG010000000
VERSION     DBMDXG010000402.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12527_length_1866_cov_1.577904"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="ACZPD3_09585"
     CDS             <1..999
                     /locus_tag="ACZPD3_09585"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR1416273.1"
                     /translation="KLQEGLYAGLLYGLDDPYSRYYTAEEYDSENSSNEGSYVGIGIL
                     MEKNKEGGVKIVECYEGGPGEKAGLEEGDIISAIDGEDITEDEVSDVADIVRNSDKDS
                     VVLTVHREGAEDAMEITVPVTDVELPSVFHEMLDSKIGYIRITQFTGVTGEQYQEAFD
                     DLQKQGMEKIIVDLRDNPGGLLDSVCDVLRKILPEGLIVYTEDKDGNREEEKCDGKNE
                     LRIPLAVLVNESSASASEIFAGAVQDYGIGTIVGTTTYGKGVVQSIRQLSDGSAIKLT
                     VANYYTPKGNNINKTGIKPDIEVSLDTSLLNKNKDEITHDEDNQLQEALKAVEKEK"
     gene            1177..>1866
                     /locus_tag="ACZPD3_09590"
     CDS             1177..>1866
                     /locus_tag="ACZPD3_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR1416274.1"
                     /translation="MSEYVITTDNNSDLPEEYLKDHGVGCMYLSYSMDGKNYTHGNFL
                     PEHEFYEAMRNGSMPTTAQVNPENAKALLEPYLKEGKDILHIAFSSALSGTYNSSRIA
                     AEELMEDYPDRKIIVVDSLGASLGQGLLVYLAQEKKEQGEDMETVAKWAEENRLHMVH
                     LFTVNDLNHLYRGGRISRTTAVVGSMLNIKPVLHVDNDGKLTAIGKVRGRKKLMDEKI
                     GSYHDTCHTIFI"
BASE COUNT          595 a          361 c          465 g          445 t
ORIGIN      
        1 aagcttcagg aagggcttta tgcaggtctt ctctatgggc tggatgatcc ttattccaga
       61 tattatacag cagaggaata cgattctgag aattcttcca atgagggaag ctatgtggga
      121 atcggcattc ttatggagaa gaacaaagaa ggcggagtaa agattgttga atgctatgag
      181 ggcggtcccg gagaaaaagc aggactggag gaaggggaca tcatcagtgc cattgacggg
      241 gaagatatca cagaggatga agtatctgat gttgcagaca ttgtccgcaa ttcagataaa
      301 gacagtgtgg tgcttactgt tcacagagaa ggtgcagaag atgccatgga aatcacagtc
      361 cctgtcacag atgtggaatt accgtctgtt ttccatgaaa tgctggattc taaaatcggc
      421 tacatccgca ttacacagtt tacaggcgtc accggcgaac agtatcagga agcttttgat
      481 gacctgcaaa aacagggaat ggagaaaata atcgtggatc tccgcgataa tccgggtgga
      541 cttcttgatt ctgtctgtga tgtcctgcgg aaaatccttc cggaaggact gatcgtttac
      601 accgaagata aggacggaaa cagagaagag gaaaaatgtg atggcaaaaa cgaactccgg
      661 attccgcttg cagtcctggt aaatgaaagc agcgccagtg catcagagat ttttgccggt
      721 gcagtgcagg attatggtat tggaacgatt gtcggaacaa ctacttacgg aaaaggcgtg
      781 gtacagtcca tccgccagct ctcagacgga agtgccatta aactgactgt tgccaactac
      841 tacactccaa aaggaaacaa catcaacaaa acaggaatca aacctgatat agaggtttct
      901 ctggatacca gcctgctgaa taagaataaa gacgagatca cccatgatga ggataatcag
      961 ctgcaggaag cgcttaaggc agttgagaag gaaaagtagt tgtttggctg atagtttgat
     1021 gttatactgt atatatctgt atttataata gaagcatttt tattatagat gaaaagccca
     1081 ttactgagca ggcagtggat tttgatttgt agttgctaag acactgaaca gttaatgggc
     1141 agaaagtgaa aaatatcaga aaaagagggc gacaacatga gcgaatatgt gatcaccacc
     1201 gacaacaatt cagatcttcc ggaggaatat ctcaaagacc atggagtagg ctgcatgtat
     1261 ctcagttatt ccatggatgg aaaaaattat acacatggaa atttccttcc ggagcatgaa
     1321 ttttatgagg caatgagaaa cggttccatg ccaaccacag cacaggtaaa tcctgaaaat
     1381 gcaaaagcac tgctggagcc ttacctgaaa gaaggaaaag atattcttca tattgcattt
     1441 tcctctgcct taagtggtac atacaatagc agccgtattg cagcagagga actgatggag
     1501 gactatccgg accgcaagat catcgtggta gattctcttg gcgcttccct tggtcagggg
     1561 cttctggttt accttgcaca ggagaagaaa gagcagggag aagatatgga gacagttgcg
     1621 aaatgggcag aagagaacag gcttcatatg gtacaccttt ttacagtcaa cgatttaaac
     1681 cacctatacc gcggaggccg catttccaga actactgcag tagtaggaag catgctgaat
     1741 attaaaccgg tccttcatgt agacaacgac ggaaaactca ctgccatcgg taaagtccgc
     1801 ggccgtaaaa agcttatgga tgagaaaatc ggcagctatc atgacacttg ccataccatc
     1861 tttatc
//
LOCUS       DBMDXG010000403         1843 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12714_length_1843_cov_1.351894, whole genome shotgun sequence.
ACCESSION   DBMDXG010000403 DBMDXG010000000
VERSION     DBMDXG010000403.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12714_length_1843_cov_1.351894"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            235..1272
                     /gene="plsX"
                     /locus_tag="ACZPD3_09595"
     CDS             235..1272
                     /gene="plsX"
                     /locus_tag="ACZPD3_09595"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR1416275.1"
                     /translation="MAEQVRVVVDAMGGDNAPAEPVKAAVEAVTERQDIKVILTGKQE
                     VIDKELAKYSGYPKNQIQVVNASQVIETAEPPVFAIRKKKDSSIVVGLNMVKKQEADA
                     FISSGSTGAVLVGGQVLVGRSKGVERPPLAPLIPTTKGVSLLIDCGANVDARPSHLVQ
                     FAKMGSIYMENVVGIKNPRVAIVNNGAEEEKGNALVKETFPLLKECKSINFIGSIEAR
                     DIPAGYADVVVCEAFVGNVILKLYEGVGSALVQKIKEGMMTSTRSKIGALLVKPALKE
                     TLKSFDATEYGGAPLLGLKGLVVKTHGSAKAIEIKHGIFQCVQFKQEKINEKIAERIL
                     EDQELLKSGAE"
     gene            1310..1537
                     /gene="acpP"
                     /locus_tag="ACZPD3_09600"
     CDS             1310..1537
                     /gene="acpP"
                     /locus_tag="ACZPD3_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR1416276.1"
                     /translation="MELEKIKAIIAEVLNIDADSITEDTTFVDDLGADSLDIFQIIMG
                     IEEEYDIELDNESVEQIQTVGDAVEAIRTIK"
     gene            1663..1806
                     /locus_tag="ACZPD3_09605"
                     /pseudo
     CDS             1663..1806
                     /locus_tag="ACZPD3_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
BASE COUNT          598 a          298 c          511 g          436 t
ORIGIN      
        1 ggtgggttta tctgaatgat ctggcggagc gaatcgaggc acaaaaaagt gaaaagtgat
       61 ttttttcaga tgctattgac tttgcagagg gttctttagt atgatacaga ttatggaaga
      121 ttgcaggagc tgctttgcag cggggcaatt tgcggatgcc gggtcagtga cagaaaatat
      181 cttttgattc tgacatctgt gagtgaacag taagtgaaca ggagatatga agatatggca
      241 gaacaggtca gagttgtagt ggacgcaatg ggcggggata atgccccggc agaacctgtc
      301 aaggctgcag tggaagctgt aacagagaga caggacataa aagtaattct tacaggaaaa
      361 caggaagtga tagataagga actggcgaaa tactccggat atccgaagaa ccagatccag
      421 gttgtcaatg catcccaggt gatcgagaca gcagagcctc cggtttttgc aatccgtaag
      481 aaaaaagatt catcaatcgt agtaggtttg aatatggtta agaaacagga agcagatgcc
      541 tttatttcat caggaagtac aggtgcggtt cttgttggag gacaggtgct ggtaggcaga
      601 agtaaaggcg tggagagacc gcctcttgca ccgttgatcc ctaccacaaa gggagtttcc
      661 cttctgatag actgcggtgc caatgtggat gcacgtccat cacatctggt acagtttgcg
      721 aagatgggtt ctatttatat ggagaatgta gtcggcatta agaatccaag agttgccatc
      781 gtaaataacg gggcggaaga ggagaaggga aatgcacttg taaaagaaac tttccccctt
      841 ctgaaagaat gcaaaagcat taatttcatc ggaagtatag aagccagaga tatcccggca
      901 ggatatgcgg atgtggtcgt atgcgaagca tttgtgggaa atgtgatcct taaattatat
      961 gagggagttg gttctgccct ggtccagaag atcaaagaag gaatgatgac cagcacaaga
     1021 agtaagatcg gtgcacttct ggtaaagcct gcactgaaag aaactctgaa atcctttgat
     1081 gcaacggaat atggcggagc accgcttctg gggcttaaag gacttgtggt aaagacgcat
     1141 gggagtgcca aagccattga gatcaaacat ggtattttcc agtgtgtaca gtttaaacag
     1201 gagaagatca acgagaagat cgcagagcgt atcctggagg atcaggaatt actgaagtcc
     1261 ggagcagaat agagcaggac aggaacagaa acaggaagga gggttgaaga tggaactgga
     1321 gaaaataaaa gctatcatag cagaggttct gaatattgat gcagattcga tcacagaaga
     1381 cactacattt gtagatgatc ttggtgcaga ctctcttgat atttttcaga ttattatggg
     1441 gattgaagag gagtatgaca ttgagctgga taatgaatct gtcgaacaga ttcagacagt
     1501 cggtgatgct gttgaagcaa ttcggacaat taaataaaaa tgtaactgtc cggcagtctg
     1561 aaatttgaca ggagcataat acaggcacac tgagcagtta caaaaaatga tgtcgcaggg
     1621 ttgtcagatg acagccctgc gcatatggag agggttagag gaatgaatag aaagacagaa
     1681 gaattagaag aaatcatagg atatcatttt aaaaataaac attatcttac acaggcactg
     1741 actcacagtt cctatgcaaa tgagaagaag ttggggaagc tgggcagcaa tgagagactt
     1801 tgcttccgat gttagatgag attgtgaaga tgacggaact gga
//
LOCUS       DBMDXG010000404         1836 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12767_length_1836_cov_1.712392, whole genome shotgun sequence.
ACCESSION   DBMDXG010000404 DBMDXG010000000
VERSION     DBMDXG010000404.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12767_length_1836_cov_1.712392"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="ACZPD3_09610"
     CDS             <1..1562
                     /locus_tag="ACZPD3_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005338479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIR1416277.1"
                     /translation="AIKMIKSKISDLDKMKIEEQKRAVRSGYDMDVLPSDLVTYGEEA
                     KNLLEDLQSRNERMFLLTALFLNTAETKQELENVVFQTAGIAQKYNCALKRLDYQQEP
                     GLMSCLPLAMNLVPIKRALTTTSTAIFVPFTTQELFMGGESIYYGLNALSNNLIMADR
                     KKLKNPNGLILGTPGAGKSFAAKREITNAFIVTKDDIIVCDPEGEYYPLFRAFHGQVI
                     RISPTSHDYINPMDINLDYADDDDPLSLKSDFILSLCELVVGGKNGLEPVEKTVIDRC
                     VRLVYQNFLSDPVPEKMPILEDLYNLLREQKEQEAQRLATALEIYVNGSLKVFNHRTN
                     VELSNRMVCFDIKDLGKQLKKLGMLIVQDQVWNRVTINRSANKSTRYYIDEFHLLLKE
                     EQTAAYSVEIWKRFRKWGGIPTGITQNVKDLLASREIENIFENSDFILMLNQASGDRQ
                     ILAKQLNISPHQLSYVTNSGEGEGLVFYGSTIIPFKDKFDNSLMLYALMTSKPDEVEK
                     RKKLGIGERDD"
BASE COUNT          561 a          339 c          473 g          463 t
ORIGIN      
        1 ctgccatcaa gatgatcaag agtaaaatct ctgacctgga caagatgaag attgaggagc
       61 agaagcgggc ggtgcgttcc ggttacgaca tggatgtgct gccgtctgac cttgtgacct
      121 atggggaaga agcgaagaac cttctggaag acctgcagtc caggaatgag aggatgttcc
      181 ttttaacggc actgttttta aatacggcag agacaaagca ggagcttgaa aatgtcgtat
      241 tccagactgc cggtattgca cagaaatata actgtgcatt gaaacgtctg gattatcagc
      301 aggagccggg gcttatgagc tgcctgccgc ttgctatgaa tcttgtgcct atcaaaaggg
      361 cacttaccac aacatctacg gcaatctttg ttccgtttac cacacaggaa ttgtttatgg
      421 gtggggaatc catttactat ggtttaaatg ccctttccaa taatctgatc atggcagaca
      481 gaaagaaact gaaaaacccg aatggtttga ttttgggtac accgggtgcc ggtaagtcat
      541 ttgcggcaaa gcgtgagatc accaacgcat ttatcgtgac aaaagatgac attatcgtgt
      601 gcgatccgga aggggagtat tatccgttgt tccgggcatt tcatggacag gttatccgca
      661 tttcaccgac cagccatgat tatatcaatc cgatggacat caatctggat tatgcagacg
      721 atgatgaccc actttcctta aaatccgatt ttatcctgtc tctttgtgag ctggtagttg
      781 gtgggaagaa tggactggag ccggtagaaa aaacagttat tgaccgttgt gtacgtctgg
      841 tgtatcagaa ttttctttct gatccagtac cggaaaagat gccgatctta gaggatttat
      901 ataatcttct ccgtgagcag aaggaacagg aggcacagcg tcttgcgact gcactggaaa
      961 tctacgttaa cggttctctg aaagtattca atcacagaac caacgtggaa ctgagtaacc
     1021 gcatggtatg ttttgacatc aaagacttgg gaaaacagct gaaaaagctt ggtatgctga
     1081 ttgtacagga tcaggtatgg aacagggtga cgattaaccg ttcagcaaat aagagtacca
     1141 gatactatat tgatgagttt catctgcttt taaaagaaga gcagacagct gcgtacagtg
     1201 tggagatctg gaagcgattc cgtaaatggg gcggtattcc gacgggtatc acacagaatg
     1261 taaaagacct gcttgccagc cgtgagatag aaaatatctt tgaaaattct gactttatcc
     1321 tgatgttaaa ccaggcatcc ggtgacaggc agattcttgc aaagcagctg aacatttcac
     1381 cacaccagct ctcttatgtt accaacagtg gagaaggcga gggactggtc ttttatggca
     1441 gtaccattat tccgtttaag gataaatttg ataattccct gatgctgtat gcgttgatga
     1501 cttctaagcc ggatgaggtg gagaagcgta aaaaacttgg tatcggagaa agagacgatt
     1561 aaaaaatata aaaaacaaaa tagaacaaaa gcaggggatg agccgggaaa ttgtttccgg
     1621 caatttttct gccgggataa agtgcaaagg ggatggtgag atttgcagga gcatgagtat
     1681 aaagcgagag ataaaaccgt ccagaagatg agccgtgatg gtcttagaga agagaatctt
     1741 cggaataaag aagaaaaaag gatcactgga cgggacatgg atacagaggt ttcccgtagt
     1801 gaaaaaaagg atgaactgga tttcagtaag agaagg
//
LOCUS       DBMDXG010000405         1817 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_12929_length_1817_cov_1.459790, whole genome shotgun sequence.
ACCESSION   DBMDXG010000405 DBMDXG010000000
VERSION     DBMDXG010000405.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12929_length_1817_cov_1.459790"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..48)
                     /locus_tag="ACZPD3_09615"
                     /pseudo
     CDS             complement(1..48)
                     /locus_tag="ACZPD3_09615"
                     /inference="COORDINATES: protein motif:HMM:NF027758.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            92..745
                     /locus_tag="ACZPD3_09620"
                     /pseudo
     CDS             92..745
                     /locus_tag="ACZPD3_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
     gene            766..1815
                     /locus_tag="ACZPD3_09625"
     CDS             766..1815
                     /locus_tag="ACZPD3_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR1416278.1"
                     /translation="MYNRKKRLFLTAVCLSLGFLTGCNVGNTKNYKQAAQDLEQGNYE
                     AALEEYETAISEGVKPAQSYRGAGVAKLKLGNYEEAITYFNDALKCDKVGKALKKDIL
                     SYRAVAYLKVKDYEAALEDCQTLAENYKMDADLYFLTGETALAMDSYEEASANFEQAY
                     GEDATYDRAIQIYGAYLNRDMEADGTRYLEAALSGTAKNAEDHCDRGRVYYYMDDYEN
                     AESELKQAIDGDNTEALVLLGMVYMDKGDSANAKAMFQQYVSQAENGAKGFNGLALCD
                     IEDGDYDSALSDIESGIHVAGAEDMQSLLFNEIVVYEKKLDFQTALQKAQEYLELYPE
                     DKTVKKELAFLKTRV"
BASE COUNT          587 a          280 c          492 g          458 t
ORIGIN      
        1 gttgaatata ggacgactgt catgccaatg agtacataag acaatagaaa caggtttccc
       61 ataaggagtg ttgtaatatc tggattttgg attgaatttc ggttttgctt atgatggtga
      121 tgcagatcgc tgtattgctg ttgaccataa gggaaatgtg gtcgacggtg ataagattat
      181 gtatgtatgt ggaaaatatc tcaaggagca gggcagatta aaagatgata cagtagtaac
      241 cactgttatg tcaaacctcg gtctgtacaa atctctggag agagagggta tgaaatatga
      301 gcagactgca gtaggagata aatatgtggc agagaatatg atggagaatg gatacagtct
      361 tggcggtgag cagtccggac atattatttt cagccgttac gctgcaacag gtgatggaat
      421 ccttacttct ctcatggtaa tggaagcgtg tgtagagaag aaagcaactc tgtgtgatct
      481 ggcaagggaa atgaaggttt atccacagct tctcagaaat gtccgggtgg cagacaagaa
      541 gactgcgaga gagaatccga aggtagttgc tgctgtagag gaagttgcaa agaaactggg
      601 aagtgatggg cgtattctgg tacgcgagag tggcacagag ccattgattc gtgttatggt
      661 agaagccgga acagatgaac tctgccttga gaatgttgat catgtagtaa aggtaatgga
      721 atccgaagga ttgctcattg actgatttac tggcaaagga attacatgta taatagaaaa
      781 aaaagattat ttctgactgc tgtctgctta agccttggat ttctgacagg ctgcaatgtg
      841 ggaaatacaa agaattataa acaggctgca caggatctgg aacagggaaa ttatgaggca
      901 gcacttgaag aatacgagac agcaatttct gaaggagtga agcctgcaca gtcgtacaga
      961 ggtgcaggtg tagcgaaact caagcttgga aactatgaag aagccataac atatttcaat
     1021 gatgcgctta aatgtgacaa agttggcaaa gcattaaaaa aagatattct gtcgtataga
     1081 gctgtggctt atctcaaagt aaaggattat gaggcagcat tggaagactg ccagactctg
     1141 gcagaaaact ataaaatgga tgcggatctg tacttcctta cgggagaaac agcattggcc
     1201 atggattcct atgaggaagc ttctgctaat tttgaacagg cctacggcga agatgcaact
     1261 tatgacagag ctatccagat ctacggggca tatctgaaca gggatatgga agcagacgga
     1321 acccgttatc tggaagctgc tttgtcagga actgcaaaaa atgcagagga tcattgtgac
     1381 cgtggcagag tttattatta tatggatgat tatgaaaatg ctgaaagtga actgaaacag
     1441 gccatagacg gcgataatac ggaggcactg gttctgcttg gtatggtata tatggataaa
     1501 ggagacagcg caaatgcaaa agcaatgttt cagcagtatg tatcacaggc agaaaacgga
     1561 gccaaaggat ttaacggact tgcactgtgt gatatagagg atggggatta tgacagtgcc
     1621 ctttctgata tagaaagcgg aatccatgtg gcaggagcgg aggatatgca gagtctgctg
     1681 tttaatgaga tcgtagtcta tgagaagaaa ctggattttc agactgcttt gcagaaagca
     1741 caggaatatc tggaattata cccggaagat aaaactgtta agaaggaact tgcatttctt
     1801 aaaacgcgtg tgtaata
//
LOCUS       DBMDXG010000406         1796 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13108_length_1796_cov_0.855457, whole genome shotgun sequence.
ACCESSION   DBMDXG010000406 DBMDXG010000000
VERSION     DBMDXG010000406.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13108_length_1796_cov_0.855457"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="ACZPD3_09630"
     CDS             complement(<1..751)
                     /locus_tag="ACZPD3_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR1416279.1"
                     /translation="MDHLRKITEEEQRILDGASEVDQGLYTSGKDFTVDSAKMLEEGK
                     LIAVRTHTRFVHFPSHRHNFIEVLYVCEGSLINIIGGKQVVIEAGELLFLNQFTRHEI
                     LPAGKNDIAINFMILPEFFDVAYTMAGSNNVLADFLVNVLRQNEEKGEYLHFRVAEVL
                     QIQNLLENMIYSLVTGRGDQNRINQTTMGLIFLYLLDSVQYAEMRVPNQYENMISMTT
                     LDYIEQKYRTATLTELCDMLHLPMHVLSKMIK"
     gene            complement(899..>1796)
                     /locus_tag="ACZPD3_09635"
     CDS             complement(899..>1796)
                     /locus_tag="ACZPD3_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR1416280.1"
                     /translation="FFEIAAASFKETVEAEGGTVEVVYPETATADAQIKVLDNLISQD
                     FDAICIAANDVNALQAKLTEAMDAGIKVSSFDSAPNKDSRQVFVNQAGTVAVGQALMD
                     AVLDISGGEGQFAILSATSQAANQNAWIDAMKKIMEEDEKYSKLELVEVAYGDDEPQK
                     STDQTAALLQNYPDLKVICAPTTVGIAAAAKYLQDNESTCKLTGLGLPSEMLEYTGDD
                     DAHSCPYFYLWDMQGLGKLSAYTTMALVKGDITGALDETFTAGDLGDYTITEADDKGT
                     EIVLGEPLQFTPENIEEYAELY"
BASE COUNT          464 a          445 c          352 g          535 t
ORIGIN      
        1 tttttatcat tttgcttaat acatgcatgg gaaggtgaag catatcgcat agctcagtca
       61 aggtggccgt gcggtatttc tgctcaatat aatccagagt tgtcatggat atcatatttt
      121 catattgatt aggcactctc atctctgcat actggacaga atccagaaga tacagaaaaa
      181 tcagtcccat ggtcgtctga ttgatccggt tctgatcccc tctgcctgtt accagagaat
      241 agatcatatt ttccagaaga ttctggatct gcagcacctc tgccacacga aagtgaagat
      301 actctccctt ttcttcattc tgccgtaata cattaacaag aaaatccgcc agtacattat
      361 tgcttcctgc catggtatag gccacatcaa aaaactccgg aagaatcata aaattaatgg
      421 caatatcatt ttttcccgca ggcaggatct catgtctggt aaactggttc agaaacagaa
      481 gctctcctgc ctcaataacc acctgctttc ctcctatgat attgataagc gagccttcgc
      541 acacataaag cacctcgata aaattatgcc tgtgagacgg aaaatgcaca aatctggtgt
      601 gggtccgcac tgcgatcagc tttccctcct ccagcatttt tgcactgtcc acagtaaagt
      661 cctttcctga tgtatacagc ccctgatcca cttctgacgc tccatcaaga atacgttgct
      721 cttcctctgt tatttttctt aaatggtcca aaagtgcctg ctgcatgttt ctaccgcctt
      781 cttactgatt ttttcttatc atagcacaaa taagaaagag acgccagctt cccggcgtcc
      841 ctcttcaaaa aggattctta aatggtatga aaaaacttct tatttttatt gctttatttt
      901 agtacagctc tgcgtactct tcaatgttct caggtgtgaa ctgaagcggc tctccaagaa
      961 caatctctgt tcctttgtca tcagcctctg taattgtata atcgccaaga tcgcctgctg
     1021 taaatgtctc gtcaagcgcg cctgtgatat cgccctttac aagtgccatg gttgtgtaag
     1081 cgctgagctt tccaaggccc tgcatatccc acagatagaa gtacgggcag gaatgagcat
     1141 cgtcatcgcc tgtgtactca agcatttcag aaggaaggcc aagacctgtc agtttacatg
     1201 tggattcatt atcctgaaga tattttgcag ctgctgcaat accgacagtt gtaggtgcgc
     1261 agataacctt cagatccggg tagttctgaa gaagcgccgc tgtctggtct gtagatttct
     1321 gaggctcatc atcaccataa gcaacttcta caagctccag tttgctgtat ttctcatctt
     1381 cttccataat tttcttcata gcgtcgatcc atgcgttctg gtttgcagcc tgggatgtgg
     1441 cagaaagaat tgcaaactgg ccttctccgc cggaaatatc aaggactgca tccataagag
     1501 cctgtcctac agccacagta cctgcctgat ttacaaatac ctgacggctg tctttattgg
     1561 gtgcagagtc aaaagaggat accttgatcc ctgcatccat agcttctgtc agttttgcct
     1621 gaagagcatt tacatcgttt gctgcaatgc agattgcatc aaaatcctgg gaaatcaggt
     1681 tgtcaagaac tttgatctga gcatcagcag ttgctgtctc cggatataca acctccactg
     1741 ttccgccttc tgcttccact gtttccttaa aggaagctgc tgcgatctca aagaac
//
LOCUS       DBMDXG010000407         1795 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13111_length_1795_cov_2.023022, whole genome shotgun sequence.
ACCESSION   DBMDXG010000407 DBMDXG010000000
VERSION     DBMDXG010000407.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13111_length_1795_cov_2.023022"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="ACZPD3_09640"
     CDS             complement(<1..1031)
                     /locus_tag="ACZPD3_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006781532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1416281.1"
                     /translation="MRKTWKKLAAMAVTASMVICGGAMGVSAATEEEKPEKVTVNVAY
                     MPDYASLNGLISAIELGYFDDENIDVQLTEFSDGPTIIQAMESGSIDVGYIGQGAHKL
                     CINGKADIFALAHVSNGDAVIGSKEKGTDTIEGLKGKTIAYSSGTSSEDILNKTLAKG
                     GMTMDDITAMDMDATNIVTAMLSGSVDACATWVPNSLKVLEELGDDGVQLADNKTFID
                     QTVSLASWIAMPKYAEENHDVLVRFARALYKGFDYRADHSHDDEVCGWIADKIKLEKN
                     SLLDQVEVADWTTSEFIRDHMDDVKGYYELQQKSFVESGDCEETPVDNYVLFDIMELA
                     EIVFGGSKLS"
     gene            complement(1125..>1795)
                     /locus_tag="ACZPD3_09645"
     CDS             complement(1125..>1795)
                     /locus_tag="ACZPD3_09645"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR1416282.1"
                     /translation="ICVVGPSGCGKSTLLNIIAGLDTPTSGAVYIDGKKIEGTGTERG
                     VVFQQYALFPWLTVLKNVMFGLKLQGKSDAEAKEIAMKYIKMVQLEDFVNHYPKELSG
                     GMKQRVAIARAYAVKPEVLLMDEPFGALDAQTRTQLQTELLKTWESERKTCFFITHDV
                     DEAIILAQRVIVMSARPGRIKDIVDIDIPYPRDQETKMSPRFMELKNHIWSQVYQEYL
                     EVRK"
BASE COUNT          469 a          435 c          289 g          602 t
ORIGIN      
        1 gatagtttcg aaccgccgaa tacaatttct gccaactcca ttatatcgaa aagaacataa
       61 ttatcaactg gtgtttcttc acagtcaccg ctctcaacaa atgatttctg ctgcagttca
      121 taatagcctt tgacatcatc catatgatcc ctgatgaatt cacttgttgt ccagtctgct
      181 acttctacct gatcaagaag ggaatttttt tcaagtttaa tcttgtcagc aatccagccg
      241 cacacttcat catcatggct gtggtctgca cgatagtcaa atcctttgta aagagctctt
      301 gcaaatctta caagtacgtc atgattttct tcagcatatt tcggcattgc aatccaactt
      361 gcaagagata cagtttgatc aataaatgtt ttgttatctg ccagctgtac accatcatct
      421 ccaagttcct caagaacttt caaagagttc ggtacccatg ttgcacacgc atcaacactg
      481 ccagaaagca ttgctgttac aatgtttgtt gcatccatat ccattgctgt gatatcatcc
      541 atcgtcatac ctccttttgc aagggtttta ttcaggatat cttcactgga tgttcctgaa
      601 gaataagcaa ttgtttttcc ttttaatcct tcaattgtat ctgttccttt ttccttactt
      661 ccgattacag catcaccatt agatacatgt gcaagtgcaa agatatcagc tttaccgttg
      721 atgcaaagtt tgtgggctcc ctgtccgatg tatccaacat caatagatcc agattccata
      781 gcctgaatga ttgtcggtcc atcggagaac tctgtaagct gtacatcaat attctcatca
      841 tcaaaatatc caagttcaat tgcagagata agaccattta aacttgcata atcaggcata
      901 taagctacat ttacagttac tttttccggt ttttcttcct ctgttgctgc acttacgccc
      961 attgctccgc cacaaatgac catagatgcg gttactgcca ttgctgctaa ctttttccat
     1021 gtttttctca tagttctact tcctttcttt tccatttttc agggtagtat ttaatatttt
     1081 ctctttattc tgaaccgttc cataccggca acggccaaaa ttcattattt tcttacttcc
     1141 agatactcct gatatacctg actccagata tgatttttca actccataaa tctcggactc
     1201 atcttagttt cctgatcacg tggatacgga atatcgatat ctacgatatc tttgatacgt
     1261 ccaggtctgg cactcataac gataactctc tgagccagaa tgatcgcctc atctacatca
     1321 tgtgtgatga agaaacatgt ttttctttca ctttcccatg ttttcagaag ttctgtctgc
     1381 agctgggttc ttgtctgggc atccagagct ccgaatggct catccataag aagaacttca
     1441 ggttttacag cataggctct tgcaattgca acacgctgtt tcataccacc ggaaagttcc
     1501 ttcggataat gatttacgaa atcttcaagc tgtaccatct tgatatattt cattgcgatt
     1561 tcttttgctt cagcatcgct cttgccctgc agtttcaatc caaacataac atttttcaga
     1621 actgtcagcc atgggaacaa tgcatactgc tggaatacaa cgcctctctc tgtacctgtt
     1681 ccctcgatct ttttgccgtc aatgtataca gctccactgg ttggagtatc cagtcctgcg
     1741 ataatattca gcaatgtaga tttaccgcat ccggaaggtc ctaccacaca gataa
//
LOCUS       DBMDXG010000408         1778 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13258_length_1778_cov_1.428742, whole genome shotgun sequence.
ACCESSION   DBMDXG010000408 DBMDXG010000000
VERSION     DBMDXG010000408.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13258_length_1778_cov_1.428742"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="ACZPD3_09650"
     CDS             <1..617
                     /locus_tag="ACZPD3_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PALP domain-containing protein"
                     /protein_id="HIR1416283.1"
                     /translation="NDNKVLYDFFRTGTYDRKRDFILTTSPSMDILISSNLERLIYRI
                     AGGDAKKCAELMQSLTAGGEYTITEDMKAQLADFYGNYCTEDETAKTIAEIFKDSHYV
                     IDTHTAVAAGVYDKYVKDTNDTTPTVIASTASPYKFTRSVMEALGADTDGKDDFALAD
                     ELSALSGVKLPQAVETIRTAPVLHNRVVDAPDMPKAVKDILGIR"
     gene            710..891
                     /gene="ssrS"
                     /locus_tag="ACZPD3_09655"
     ncRNA           710..891
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACZPD3_09655"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            970..>1778
                     /locus_tag="ACZPD3_09660"
     CDS             970..>1778
                     /locus_tag="ACZPD3_09660"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR1416284.1"
                     /translation="MSVSERKFGILQTGETVKIFHLENKSGAYAEVTDFGAILVKVCV
                     PDKDGTLTDVVLGYDDLASYEVNGCFFGSTIGRNGNRIGGAKFSVNGKEVVLAQNEND
                     NNLHSGPDGFEKKLWKVAEISDDKNSVIFNRISPDGENGFPGEFDVSVKYEFTEDNEL
                     RIHYQGICDEPTVANMTNHSYFNLNGEGSGTAMDQYLTIHAKYYTPVADSHSIPTGVY
                     GEVAGTPMDFRTAKKIGQDIEAEFDQLKFTGGYDHNYVTDNYAKGNRRLIAT"
BASE COUNT          579 a          340 c          411 g          448 t
ORIGIN      
        1 ccaatgataa caaggttctg tatgatttct tccgcacagg aacttatgac agaaagcgtg
       61 actttatcct tacaacctca ccgtccatgg atattctgat ctccagcaat ctggaacgtc
      121 tgatctaccg tatcgcaggc ggtgatgcaa aaaaatgtgc agagctgatg cagtctctta
      181 ctgctggcgg cgagtatacc atcacagaag acatgaaggc acagcttgca gacttctatg
      241 gcaattactg tacagaagac gaaacagcaa aaacaatcgc agaaatcttc aaagacagtc
      301 attatgtgat agatacccat actgcagttg cagcaggtgt ttatgacaaa tatgtaaaag
      361 ataccaatga taccacacca actgtcattg catccacagc aagcccatat aaatttacaa
      421 gaagtgtaat ggaagcactt ggagcagata cagacggtaa ggatgatttt gcactggctg
      481 atgaactgag tgcgctttcc ggagtgaaac ttccacaggc agttgagacg atccgcacag
      541 caccggtact tcataaccgg gtggtagatg cgccggatat gccaaaggca gtaaaagata
      601 tccttggaat ccgataatct ccatattata aaaaaatata attaattcta cattttatgt
      661 tgaaaaagaa catatactgg agtataataa aaatcagtta aaaacagtga atatcctgaa
      721 atgtacgagg ccccttcttt ttttgaacct acatcgactt taacgggaac ccaacatcgc
      781 aggccggatg tcaggccgtc attatgcagc ggaagacaga aggtctgttg aggataccca
      841 cctagctgcg gctaggcgtc aatgggaagg gaacggcatt tcgggaactg taaaaatgag
      901 ccgtagagag gctctttttt attatctttt ggatttaata ttattttatg atacgggagg
      961 ttggaaacaa tgagtgtttc tgaaagaaag ttcggcatat tacagactgg ggaaacagta
     1021 aaaatttttc atctggaaaa caaatccggg gcatatgcgg aagtgacaga ttttggagca
     1081 atcctggtta aggtctgcgt acctgataaa gatggtactt taacagacgt agtacttgga
     1141 tatgatgatc tggcatctta tgaagtaaat ggatgctttt tcggttcaac catcggcaga
     1201 aacggaaacc gcatcggcgg tgcgaaattc tctgtcaatg gcaaagaagt agttcttgct
     1261 cagaatgaga atgacaataa tcttcacagc ggtcctgacg gatttgaaaa aaagctctgg
     1321 aaagtggctg agatttccga tgataaaaac agtgttatat ttaaccgtat aagtccggat
     1381 ggcgagaatg gtttcccggg agaatttgat gtgtctgtaa aatatgagtt tacggaagac
     1441 aacgaactga ggatccatta tcagggaatc tgcgatgaac cgacagttgc caatatgaca
     1501 aatcattctt atttcaatct gaatggtgag ggaagcggaa ccgccatgga tcagtatctt
     1561 acaatccatg caaaatatta tacacctgta gccgacagtc attccattcc tacaggagtg
     1621 tatggggaag ttgcagggac tcccatggat ttcagaactg ccaaaaagat cgggcaggat
     1681 attgaagcag aatttgacca gctgaaattc acaggcggat atgaccacaa ttatgtgaca
     1741 gacaattatg caaaaggaaa cagaagactg atcgcaac
//
LOCUS       DBMDXG010000409         1764 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13391_length_1764_cov_1.835238, whole genome shotgun sequence.
ACCESSION   DBMDXG010000409 DBMDXG010000000
VERSION     DBMDXG010000409.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13391_length_1764_cov_1.835238"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            111..881
                     /locus_tag="ACZPD3_09665"
     CDS             111..881
                     /locus_tag="ACZPD3_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1416285.1"
                     /translation="MGHVIAISGKGGVGKTTLCGLLIQYLCESGKCPVLAVDADANAN
                     LNEVLGVEPEITLGELREEIERAGVDPRYQIPSGMTKQAYLEMRLSDAIAEENDYDLM
                     VMGRTQGQGCYCFVNGLVQTQVQKLQSHYPYIVVDNEAGMEHISRGILPMMEVAILVS
                     DCSRRGVQAAGRIAKLMNELNFKPQKTGLIVNRVPDGKLDAGTLEEIRNQGLELLGVV
                     PHDNQVYQYDCDGKPIIQLPKDSPVRSALGEIVKKLGL"
     gene            995..1171
                     /locus_tag="ACZPD3_09670"
     CDS             995..1171
                     /locus_tag="ACZPD3_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416286.1"
                     /translation="MGFFSGCMDARNMTSIEEVKCPKCSADIEVFVRDGKTVGDSVCD
                     NCGYTIKENQIIQV"
     gene            1318..>1764
                     /locus_tag="ACZPD3_09675"
     CDS             1318..>1764
                     /locus_tag="ACZPD3_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR1416287.1"
                     /translation="MIQIAIVEDEEIYVKQLTEYIRKYQTERGRSIKVTVFGDGEDIT
                     ENYSGGFDIILMDIQMRFMDGMTAAEKIRQMDQKVIIMFITNMIQYAVRGYEVDAMDY
                     VVKPVEYFSFSQKLDKAVGRMRSEVKEFLTIPIGEGVVKINIADIYF"
BASE COUNT          618 a          241 c          443 g          462 t
ORIGIN      
        1 gaggagccat acaaatctca cagattgatt attgtaattt atatagtaac aactataata
       61 agatttaaag aaaaagtcaa cagattgttc agaatattag gaggaaaaac atgggacatg
      121 taattgcgat atcaggaaaa ggcggcgtag gaaaaactac actttgtgga ttattgatcc
      181 agtatttgtg tgaaagtggg aaatgtccgg ttctggccgt ggatgcagat gcgaatgcca
      241 atttaaatga agtgcttggt gtggagccgg agatcacact tggagagctg agagaagaaa
      301 ttgagcgggc aggcgtagac ccacgatatc agatcccatc cggtatgacg aagcaggcat
      361 atctggaaat gcgtctttct gatgcgattg cagaagaaaa tgattatgat ctgatggtaa
      421 tggggcgaac ccagggacag ggctgctatt gctttgtaaa tggtcttgta cagacccagg
      481 ttcagaaact gcaaagccat tatccatata ttgtggttga taacgaagca ggtatggaac
      541 atatcagcag aggaatcctg ccaatgatgg aagttgccat tcttgtaagt gactgttcca
      601 gaagaggtgt tcaggcagcc ggacgtattg caaagctgat gaatgaatta aactttaaac
      661 cacagaaaac tggattaatc gtgaatcgtg ttccggatgg aaaactggat gccggaactc
      721 tggaagaaat ccgaaatcag ggactggagc tgctgggagt ggtgcctcat gataaccagg
      781 tttatcagta tgactgtgat ggaaagccga ttatacagct tccgaaagat tctccggtca
      841 gaagtgcact gggagaaatt gtaaagaagc taggactgtg agagagggag gttctcttta
      901 tgatttcagc cagacagcag tagatgagat aaaaaaataa aagaaaaatt aggttaagaa
      961 aataatagaa tatatacagg aaaaaggtga aaagatggga tttttttcag gatgtatgga
     1021 cgccagaaac atgacttcaa ttgaagaagt taaatgtccg aaatgtagtg cagatataga
     1081 agtatttgtg agagatggaa aaacagttgg cgacagtgta tgcgataact gtggatatac
     1141 gataaaagaa aaccagatta tacaggtgta atttgtaata ttacatccac ttgactcagg
     1201 aatgaagcag tgatatacta aaacaaaagt tatttataaa ataacagtaa aaacctggaa
     1261 ctatattgtg cgggcttgaa cagttacaaa aaatgcaatt cttgtggagg gtataggatg
     1321 atacagattg caattgtgga agatgaagaa atttacgtaa aacaattaac agagtatatc
     1381 cggaaatatc aaacggaaag gggaagatca ataaaggtta cggtatttgg tgatggagaa
     1441 gatatcactg agaattacag tggcggtttt gacattattc tgatggatat tcagatgcgg
     1501 ttcatggacg gtatgacagc agcagaaaaa atccgtcaga tggatcagaa agtaattatt
     1561 atgtttatta caaatatgat tcaatatgca gtacgtggat atgaagttga tgcaatggat
     1621 tatgtagtaa aaccagttga gtacttttct ttttcacaga aactggataa agcagttgga
     1681 cgtatgcgct ctgaggttaa agaatttctt acgattccga ttggggaagg agttgtaaag
     1741 ataaatatag cagatatcta tttt
//
LOCUS       DBMDXG010000410         1752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13497_length_1752_cov_1.059964, whole genome shotgun sequence.
ACCESSION   DBMDXG010000410 DBMDXG010000000
VERSION     DBMDXG010000410.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13497_length_1752_cov_1.059964"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            147..713
                     /locus_tag="ACZPD3_09680"
     CDS             147..713
                     /locus_tag="ACZPD3_09680"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR1416288.1"
                     /translation="MDKDYTILINRKHPLPEDFIPEHLVDIGLPFDAVPGEPKRLLEE
                     KTARSALELITRSRSEGLNLYCISGYRSYRRQKELFTGDSYVAPPGSSEHQSGLALDM
                     SCPAVQMELTEAFAETKEGIWLNRHAALFGFIIRYPKNKEHITGIPWEPWHIRYVTRS
                     LASYLSLTGLTLEEYHMLENPVDFSNLK"
     gene            996..>1752
                     /locus_tag="ACZPD3_09685"
     CDS             996..>1752
                     /locus_tag="ACZPD3_09685"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR1416289.1"
                     /translation="MSMNINHQLPLPDVLKEQYPLTDELRSVKSLRDSEIRKIFTGES
                     DKFVVLVGPCSADNEDTVCEYANRLKKVNDQVSDKLMIIPRVYTNKPRTTGDGYKGML
                     HQPEPDKAPDLLAGIIAIRKMHIRVLAETGLSTADEMLYPENRSYLDDILSYEAIGAR
                     SVENQQHRLTASGMDIPVGMKNPTSGDLSVMLNSVTAAQHSHHFIYRGFDVATSGNEL
                     AHTILRGGVNKFGQTQPNYHYEELVRLAELYNQW"
BASE COUNT          501 a          424 c          362 g          465 t
ORIGIN      
        1 acccccagaa agtttatgta gatctttctc agaagctcta tgatctgaaa aaaaatcttc
       61 tggaggaaat gagcgaaaag gcccttgatt aaaggggcat atcccctccc tgcctttcat
      121 atcataaaag gtaagggggg atttttatgg acaaagatta cacgattctc attaacagga
      181 agcatccgct tcctgaagat tttataccgg agcatctggt tgatatcgga ctgccctttg
      241 atgctgttcc cggagagcct aagcgtcttc tggaagaaaa aacagcccgc tccgctcttg
      301 agctgatcac acgcagccgc tccgagggac tgaatctgta ctgtatctcc ggttaccgtt
      361 cttacaggcg tcagaaagaa ctctttacag gtgattctta tgtggcgcct ccgggctcca
      421 gtgagcatca gagcggtctt gcgctggaca tgtcctgtcc tgcagtacaa atggagctga
      481 ctgaagcctt tgcagaaaca aaagagggaa tctggctgaa tcgccatgcc gcgctttttg
      541 gttttattat acgatatccc aaaaataaag aacatatcac cggtattcca tgggaaccct
      601 ggcatatccg ctatgtaacc agatctcttg cctcttatct ttcactgacc ggtctgaccc
      661 tggaagaata tcatatgctt gaaaatccgg tggacttttc caatttaaag tgatatactg
      721 tgaatgtttc gtaaataaaa gtttccactg ttgtttacac agtactgtcc cgcaggtttt
      781 cccacatttt attaaaaatc cgaaaccctg cagactgaat tttttatgaa aataatgtag
      841 aacatatttt aattgctgct gcaaacaaaa ccttactgtt catgaccttc acagcaactt
      901 ggtcaaatgc catgtgaact gtaacaataa aacactttgc agcttatctg cagttaacta
      961 ttttataact aatctactta cggaggaaat ctatcatgtc aatgaacatc aaccaccagc
     1021 ttcctttacc tgatgtgctg aaggaacagt atcccttaac ggatgagctc agatcagtaa
     1081 aatctctgag agacagcgag atccgcaaaa tttttaccgg agagtctgat aaatttgttg
     1141 ttctggtcgg tccctgctct gcagacaacg aagatactgt atgcgaatac gcaaaccgtc
     1201 tcaaaaaagt aaacgaccag gtttccgata aactgatgat cattccgaga gtctatacca
     1261 ataagccccg caccacaggc gacggctaca aaggcatgct ccatcagccg gaaccggaca
     1321 aggctcctga tctcctggca ggcatcattg ccatccgcaa aatgcatatc cgtgtgctgg
     1381 cagaaaccgg actctctacc gcagacgaaa tgctttatcc tgaaaatcgc agctatctgg
     1441 acgatattct gtcttatgaa gctatcggcg cccgctctgt agaaaaccag cagcaccgcc
     1501 ttactgcaag tggaatggat atccctgtgg gaatgaaaaa tcctaccagc ggagatcttt
     1561 ctgttatgtt aaactccgtt accgctgccc agcattccca ccattttatt taccggggat
     1621 ttgatgttgc taccagcgga aacgagcttg cccacaccat cctcagaggc ggcgtaaata
     1681 aattcggcca gactcagcca aattatcact atgaagagct tgtacgcctt gcagaattat
     1741 ataatcagtg ga
//
LOCUS       DBMDXG010000411         1748 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13534_length_1748_cov_1.345477, whole genome shotgun sequence.
ACCESSION   DBMDXG010000411 DBMDXG010000000
VERSION     DBMDXG010000411.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13534_length_1748_cov_1.345477"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="ACZPD3_09690"
     CDS             <1..905
                     /locus_tag="ACZPD3_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR1416290.1"
                     /translation="NQIVDNIQSRLEEIGKEKDVNFDVSYDNCNADANVMEQIISDFQ
                     ADNVDLMVGIATPVAMRMQSATEGTDTPVVFSAVSDPVGSGLVEDLDAPGANITGTSD
                     YLDTASIMKLIQAVNPDVKKIGLLYDIGQDSSTTAIQEAKDYLDKEGIEYVERTGTTT
                     DEVQLAADALVADGVDAVFTPTDNTIMTAELSIYEKFIDAGIPQYTGADSFALNGAFV
                     GYGVDYANLGQKTADMIADILMNDADPATTSVETFDNGTATVNTETCEGLGLDLETVK
                     KDFEPLCTQVNEIVTAESFDDVEK"
     gene            1081..>1748
                     /locus_tag="ACZPD3_09695"
     CDS             1081..>1748
                     /locus_tag="ACZPD3_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR1416291.1"
                     /translation="MTIATILSLGETALKLGLICSLTVLALFLSYSMLNVCDLSTDGC
                     FTLGAAVGAAVAVSGHPFLSIFAAMAAGICSGFVTAILQTKLGVDSLLAGIIVNTALY
                     SVNIAVMGGSSLINMNRTETVFSMTKETLKSTPLKGRGDIVVAFIAVAIVIVFLVFFL
                     RTRLGLAIRATGNNADMVKSSSINPVFTTIVGLCVANSFTALSGCLLSQSQKSVDINI
                     GQGMV"
BASE COUNT          524 a          329 c          406 g          489 t
ORIGIN      
        1 tgaatcagat cgtagacaac atccagtccc gtctggaaga aatcggcaag gaaaaagacg
       61 taaattttga tgtgtcctat gataactgta atgcagatgc caatgttatg gaacagatca
      121 tttctgattt tcaggctgat aatgtagatt tgatggtagg tattgcaaca cctgtagcaa
      181 tgcgtatgca gtccgcaaca gaaggaaccg atacaccggt tgtattttcc gcagtgtctg
      241 atcctgtagg aagcggcctt gtagaggatc tggatgcacc gggagcaaac attacaggta
      301 caagcgatta tctggataca gcatccatca tgaaactgat ccaggcagtg aacccggatg
      361 taaagaaaat cggactttta tacgatatcg gacaggattc ttctacaaca gccatccagg
      421 aagccaagga ttatctggat aaagaaggca tcgaatatgt agagcgtaca ggtaccacaa
      481 cagatgaggt acagcttgca gcagatgcac tggtggcaga tggcgtagat gcagtattca
      541 caccaactga caacacaatt atgacagcag agctttctat ctatgagaaa ttcattgatg
      601 caggtatccc acagtataca ggagctgact cctttgcact aaacggtgca tttgtcggat
      661 atggtgttga ttatgcaaac cttggacaga agacagcaga tatgatcgca gacatcctta
      721 tgaatgatgc agatcctgca acaacatctg ttgaaacttt tgataatggt actgcaacag
      781 taaacacaga aacctgcgag ggactgggac ttgatcttga aactgttaaa aaagatttcg
      841 agccgttatg cacacaggta aatgagatcg ttacagcaga gagctttgat gatgtagaga
      901 aataataagg atattcggac agcattttga ataaattcag aataagttct gggtttatgg
      961 aatccattgt gccggttgaa aagactgata tattttctga attaaaatga acagcagcag
     1021 atattttctg ttgctgttca ttgaatgtaa taaaataaat acaaactgga ggaaatcatc
     1081 gtgactattg cgactatact ctctcttgga gagaccgcgc tgaaacttgg cctgatctgt
     1141 tcattgacag ttcttgccct gtttctcagt tattccatgc tgaatgtctg tgacctttcc
     1201 actgatggct gttttacact gggagcagca gtgggagcag cagtggcagt cagcggacat
     1261 ccttttttat caatttttgc agctatggct gcaggtatat gctcgggctt tgtgacagcc
     1321 atacttcaga caaagctggg tgttgacagt cttcttgcag gaattatcgt taatacggct
     1381 ctttattctg tgaatattgc agtgatggga ggttcctccc tgatcaatat gaacagaaca
     1441 gagacggtat tttccatgac gaaagaaaca ttgaaaagta caccgctgaa aggaagagga
     1501 gatattgtag ttgctttcat tgcagtagca attgtgattg tcttccttgt atttttctta
     1561 aggacaagac ttggtctggc aatcagggca acaggaaata atgcagatat ggtaaaatcc
     1621 tcatctatta atccggtatt tactacgatt gtaggtctct gtgttgccaa ctccttcaca
     1681 gcactttccg gatgtcttct gtctcagtca cagaaatctg tagatatcaa tatcggacag
     1741 ggaatggt
//
LOCUS       DBMDXG010000412         1740 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13626_length_1740_cov_0.973764, whole genome shotgun sequence.
ACCESSION   DBMDXG010000412 DBMDXG010000000
VERSION     DBMDXG010000412.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13626_length_1740_cov_0.973764"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            466..765
                     /locus_tag="ACZPD3_09700"
     CDS             466..765
                     /locus_tag="ACZPD3_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1416292.1"
                     /translation="MAKNYDGMAVGAFELDNLVACFVALDAASKAANVTIQSVERNRL
                     KSGACVKIRGSVSDVNAAMEVALETAKPLGKIVSHTVIASPSADTEVALKMTINK"
     gene            790..1077
                     /locus_tag="ACZPD3_09705"
     CDS             790..1077
                     /locus_tag="ACZPD3_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR1416293.1"
                     /translation="MSYGALGLVEVVGSCNAVVVLDQMLKTSDVEFRTWHTKCGGHAT
                     IFLSGDIAAVKAAVDAVKGNPPCEIIAAAVISAPSEETSRLVKEEEEKHHK"
     gene            1224..1724
                     /locus_tag="ACZPD3_09710"
     CDS             1224..1724
                     /locus_tag="ACZPD3_09710"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR1416294.1"
                     /translation="MDYKSVKISEDAKIARQSVVIGDVTIGRDSCVLHYAVIRGDDAP
                     IVIGEESNVQENCTIHVSRNMPVHIGNNVTVGHNAVLHGCMIGDRTLIGMGAVVLDGA
                     RIGKDCIIGAGSLVTKNTVIPDGSLVMGSPAKIKRNLTWEEKLGNLENSKEYVSSNKE
                     SYFCAD"
BASE COUNT          590 a          266 c          430 g          454 t
ORIGIN      
        1 cagaatatta cagttacagc tgaaattata ttcagatatg gaatatcctg aaaatattgc
       61 agatttagaa aaaatttaac tgaatcaagt aagtcctctg cttcaattgc aaatatccgt
      121 ctgaccctac agcatcaaat gttacagcag gaggtaccgg ataattccgg gtcatttgat
      181 gcatgacgat taaaacctga caacagcaga ttgttgttct ccatattagt atatgttctg
      241 catagaagcc tgtgaatggt tgtaagagag aagatttatg aaaatagtga aaaataaaca
      301 atccaacaga taaactcttg aagatgatgt ggtattgtgt ggcattatgt gaatttttgt
      361 gagatttcag tagaaaatag cttgtgatta taatgaaaac aaggtatact agtataaaag
      421 acagcagtcg ggagaaccat tttcccgtct cgtaaggagg ataaaatggc aaaaaactat
      481 gatggcatgg cagttggggc atttgaactg gataacctgg tagcttgctt tgtcgctctc
      541 gatgcagcat ccaaggctgc aaacgtaacg attcaaagcg tcgaacgaaa ccgactgaaa
      601 agcggagcat gtgtaaaaat acgcggcagt gtttctgatg tgaatgcagc aatggaagtg
      661 gccttagaga cagccaaacc ccttggcaag attgtttctc acacagtgat cgcatcacct
      721 tccgcagata ctgaagtggc acttaagatg accattaaca aatagaacat gtcaggagga
      781 aatgagaaga tgtcatatgg agcattagga ttagtagagg ttgtgggaag ctgtaatgca
      841 gtagttgtcc ttgaccagat gctgaaaaca tcagatgtgg aattccgtac ctggcatacc
      901 aagtgcggag gtcacgcaac tatattctta agtggcgata tagcagcagt aaaggcagca
      961 gtagatgctg taaagggaaa cccgccctgt gaaattatag cagcagctgt aatctcagca
     1021 ccgtctgaag aaacatcaag actggtgaaa gaagaggaag aaaaacacca taaataatag
     1081 ctaagaggac tttctgtaaa tcagtaaatg aaatgcagaa agtctttttg gaatatgtat
     1141 aaagattctg cagtaaagaa gtgtcagggt attgaaaatg atgtgaaggt agtattaaat
     1201 caagaaggca ggtatttcgg aagatggatt ataagagtgt aaaaatatca gaagatgcaa
     1261 aaattgcaag gcagtcagtg gttattggag atgtaactat cgggcgtgac agttgtgttc
     1321 ttcattatgc tgtgatacgt ggcgatgatg ctccgatcgt gattggtgag gaatctaatg
     1381 ttcaggaaaa ctgtacgatt catgtaagcc gtaatatgcc ggtacatatt gggaataatg
     1441 tgacagttgg acataatgca gtgcttcatg gatgtatgat cggtgaccgt acattgatcg
     1501 gaatgggagc ggttgttctt gatggtgcca gaataggtaa ggattgtatc ataggagcag
     1561 gaagtcttgt gacaaagaat acagtgatac cggacggttc tctggtgatg ggaagtcctg
     1621 caaagattaa acgaaacctt acatgggaag aaaagctggg aaatcttgaa aacagtaaag
     1681 agtatgtttc ttcgaataaa gagtcgtatt tttgcgcaga ttaaaatagg aatcaaacaa
//
LOCUS       DBMDXG010000413         1723 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_13799_length_1723_cov_1.437731, whole genome shotgun sequence.
ACCESSION   DBMDXG010000413 DBMDXG010000000
VERSION     DBMDXG010000413.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_1723_cov_1.437731"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(78..443)
                     /locus_tag="ACZPD3_09715"
     CDS             complement(78..443)
                     /locus_tag="ACZPD3_09715"
                     /inference="COORDINATES: protein motif:HMM:NF014573.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR1416295.1"
                     /translation="MAEILGMFRKADVLVLATPVYFYGISAQMKTFIDRTYPIWQHLG
                     KKEVYYIISAGLGEDIIERPLGDLDGFVEHLEEYKIAGKIYAANVMDAGLVRNQSVFK
                     KAYDMGYSVQNAEKLRVGE"
     gene            complement(445..648)
                     /locus_tag="ACZPD3_09720"
     CDS             complement(445..648)
                     /locus_tag="ACZPD3_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416296.1"
                     /translation="MTFFTNSKKYAKKIKKAEKCVVYENTVRELFSCEQFKKGAEAKG
                     HQVNMVQSLRRLYEKWRYLCIKR"
     gene            complement(735..1508)
                     /locus_tag="ACZPD3_09725"
     CDS             complement(735..1508)
                     /locus_tag="ACZPD3_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR1416297.1"
                     /translation="MPRPIKCRKVCHFPNVLEFLPADDTEKKAPIILTVDEYETIRLL
                     DKKGYSQEQCAVSMQIARTTVQRIYEIARKKIADALIDGHPLRIEGGDFRICDGQSSD
                     CNLGGCYKQELYQKYAVEKGEGIMRIAVTYENGQIFQHFGHTETFKIYDVEEGKVVHS
                     EVVDTNGSGHGALAGVLNALNADVLICGGIGGGAQTALAAAGIKLFGGVSGDADEAVE
                     AFINETLAYNPNVKCSHHEHNHGEGHTCGEHGCVSHSCH"
BASE COUNT          453 a          420 c          283 g          567 t
ORIGIN      
        1 attccggggc aaaaataact acttgaattt ttttgctttt atgacatcta taattatccc
       61 tttgtttttt ataagcttta ttcgccaact ctcaatttct cagcattttg aacggaatat
      121 cccatatcat aggctttttt gaaaacactt tgatttctga ctaatccggc atccatcacg
      181 tttgctgcat atatctttcc tgcaatcttg tattcttcca gatgttctac aaaaccatcc
      241 agatcaccaa gcggtctttc aatgatgtcc tcgcccagac cagccgatat aatatagtaa
      301 acttcttttt taccaagatg ctgccagata ggataagtgc ggtcaataaa ggtcttcatc
      361 tgggcactga ttccataaaa ataaaccgga gtagccagta caagtacatc tgctttccga
      421 aacattccga gtatttcagc catgtcatct tttaatacac aggtaccgcc atttctcata
      481 cagccgtcgc aggctctgta ccatgttcac ctgatgtcct tttgcttctg cacctttttt
      541 gaactgctca cagctaaaca gttctctgac tgtattttca tataccacac atttctctgc
      601 cttcttaatc tttttggcat attttttact gttagtaaag aatgtcatgg ttcgacccta
      661 tgaagtacgg ccctataaag tatagatatt tatctacaat ctcccagtag ccgctcttag
      721 gcgaaccatt cttcttaatg acagctatgg cttacgcatc catgctcgcc acatgtatgt
      781 ccttccccgt ggttgtgctc gtgatgagaa cacttcacat ttggattata cgcaagtgtt
      841 tcattgataa aggcttctac cgcttcatct gcatctccgg aaactcctcc gaaaagctta
      901 ataccggctg ccgctaaagc tgtctgtgcg ccccctccga ttccgccaca gatcaaaaca
      961 tctgcattca atgcattaag aactcctgcc aatgcgccat gtccgcttcc gttggtatct
     1021 actacctctg aatgtaccac tttcccttct tctacatcat aaatcttaaa tgtttctgtg
     1081 tgtccaaagt gctggaaaat ctgtccgttt tcatatgtta ctgctattct cataatacct
     1141 tctccttttt ccactgcata tttttgataa agctcctgct tgtaacatcc tccaaggttg
     1201 cagtcgctac tttgaccatc gcagattctg aaatccccgc cctcaattct aagcggatgt
     1261 ccatcaataa gtgcatctgc tattttcttc ctggcaatct cgtaaattcg ttgaactgtt
     1321 gttcttgcga tctgcataga tactgcacac tgctcctgac tgtaaccttt cttgtccaaa
     1381 agacgaattg tttcatactc atctaccgtc agaataatgg gggctttttt ctccgtatca
     1441 tctgccggaa gaaattctaa aacattggga aaatggcaga cttttctgca ttttattggt
     1501 cttggcataa actgctcctt tcttgttata atataatagt tctataaagg gcatatgtca
     1561 ataacatttt tgacatatgc ctttttaacc aaatcaagta agtcccctgc ttcaattgcg
     1621 aagagcaaac gggcatcgga gagacttctg gaaagcagta cgaaatatct tgaagagaag
     1681 ctgaaactca cagtcaacag agagaagctt aatgagatac tga
//
LOCUS       DBMDXG010000414         1702 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_14008_length_1702_cov_1.004372, whole genome shotgun sequence.
ACCESSION   DBMDXG010000414 DBMDXG010000000
VERSION     DBMDXG010000414.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14008_length_1702_cov_1.004372"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            32..460
                     /locus_tag="ACZPD3_09730"
     CDS             32..460
                     /locus_tag="ACZPD3_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR1416298.1"
                     /translation="MSDTHGLLRTEVLDILQSCDCIFHAGDVDRPELLDTIRSLGALY
                     VVRGNNDGYWAQNLRRSLNFTVGNVKFFMVHDRKDVAWELGDTQVVIFGHSHQYFAKE
                     IDGRLWLNPGSCGRSRFGGDVTMAVMTVENGSWQVEKIVL"
     gene            630..1106
                     /locus_tag="ACZPD3_09735"
     CDS             630..1106
                     /locus_tag="ACZPD3_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416299.1"
                     /translation="MEKKEIDVKFIVGIVILLVIFGVVGGSFWNLVARQAQKDKQEEA
                     RLENEAIRAIYVEAGDILKEMVFVDMDKKTVFKAAIPKEGIYNKNDKLIAGDTLENGD
                     MVKIYGDRNMTRSIPAQYPGITKMKRNGRASLEELQPYLEIANELLCGDSEEEDKK"
     gene            1103..1465
                     /locus_tag="ACZPD3_09740"
     CDS             1103..1465
                     /locus_tag="ACZPD3_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR1416300.1"
                     /translation="MKYLRQFCIILLVSALGEGLHILLPLPVPASVYGLVLMLGALAS
                     GILKLEQVEEAADFLVEIMPVMFVPAGVGLLEAWGDLQPIWLPVVLITILTTVIVMVV
                     TGRSTQFVIRKDRGKDKK"
     gene            1462..>1702
                     /locus_tag="ACZPD3_09745"
     CDS             1462..>1702
                     /locus_tag="ACZPD3_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR1416301.1"
                     /translation="MINFIENSLFFGAALSLAGYEVGLLLKKKFKMALFNPLLIAILC
                     VMGMLQLMQVDYKTYNAGGQYISYLLTPATVCLAVP"
BASE COUNT          517 a          262 c          458 g          465 t
ORIGIN      
        1 gacagaaaat aagaaaatta aaattgggat tatttcagat actcatggtc ttttacggac
       61 agaagttctg gatatattac agtcctgtga ctgcattttt catgctggag atgtggacag
      121 accggagctt ctggatacta taagatcatt gggcgcttta tatgtagtgc gaggaaacaa
      181 tgatggatac tgggcacaga atctgcgaag aagcttgaac tttacagtgg gaaatgtgaa
      241 attctttatg gttcatgacc ggaaagatgt tgcctgggag ttaggggata cccaggtggt
      301 gatttttgga cacagtcatc agtattttgc aaaagaaatt gacggaaggc tgtggctgaa
      361 ccctggaagc tgcggcagaa gccgctttgg gggagatgtg accatggcag ttatgaccgt
      421 ggaaaacggc agctggcagg tggaaaaaat cgtgctctga acagttgcaa tgtggaaaaa
      481 aggaatatcc agaacacagt tttacaatta tgttgtagaa aaattgggaa aaacagggga
      541 gagaattgtc gaaagatgac attgcaattt taacaagata atgtataata gggcagaaga
      601 aaaagccatg aaagaaatga ggatttgcag tggagaaaaa agaaatcgat gtaaaattca
      661 ttgtaggaat tgtcattctt ttggtgatct ttggcgtagt aggcggctcg ttctggaatc
      721 tggttgccag acaggcacag aaagacaaac aggaggaagc ccgtctggaa aatgaagcca
      781 taagagctat ttatgtggaa gccggagata tactgaagga aatggtattc gtagatatgg
      841 ataaaaaaac tgttttcaag gcagcgattc ccaaggaagg aatttataac aaaaatgata
      901 aattaattgc cggggatacc ttggaaaatg gagatatggt gaaaatctac ggtgacagaa
      961 atatgacaag atccatccct gcacagtatc cagggattac aaagatgaag cgaaatggca
     1021 gggcttctct tgaggagctt cagccttatc tggaaattgc aaatgaactt ctttgtggag
     1081 acagcgagga ggaagacaaa aagtgaagta tctgaggcaa ttttgtatta ttttgctggt
     1141 ttcagcgttg ggtgagggat tacatatcct tttgcctctt ccggtaccgg ccagtgtata
     1201 tggtcttgtg ctgatgcttg gagcacttgc aagcggaatt ctgaaacttg agcaggtaga
     1261 ggaagcagca gattttctgg ttgaaattat gccggttatg ttcgttcctg caggtgtggg
     1321 acttttggaa gcgtggggtg atctgcagcc aatctggctt cctgttgtgc tcataacaat
     1381 cctgacaacc gtcattgtaa tggtagttac tggacgcagt actcagtttg tgatcagaaa
     1441 ggacaggggg aaagataaga aatgataaat tttatagaaa attccctgtt tttcggggcg
     1501 gcattaagtc tggctggata tgaggtcggt cttttgctga agaaaaagtt taaaatggcg
     1561 ctgtttaatc ctcttttgat tgcaattcta tgtgtaatgg gaatgcttca gcttatgcag
     1621 gtggattata agacctacaa tgcaggcggt cagtatatca gttatctgct tactcctgct
     1681 acagtatgtc ttgcggtacc gc
//
LOCUS       DBMDXG010000415         1697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_14056_length_1697_cov_1.807644, whole genome shotgun sequence.
ACCESSION   DBMDXG010000415 DBMDXG010000000
VERSION     DBMDXG010000415.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14056_length_1697_cov_1.807644"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="ACZPD3_09750"
     CDS             <1..278
                     /locus_tag="ACZPD3_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HIR1416302.1"
                     /translation="EIKKNNLAIRGSKEPRSPYGTFQNVFLSEKEMEDIQQTIPDWQD
                     YMERLSGYMASTGKQYQNHAATIISWARQDHPASRQRNYESEEYETL"
     gene            275..1132
                     /locus_tag="ACZPD3_09755"
     CDS             275..1132
                     /locus_tag="ACZPD3_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR1416303.1"
                     /translation="MTRFTEKENAITPNRELQPDEYFNETDHLIYCSKCNTPRQCRHE
                     LQGKVLIPSIRCKCQQEIFEQEEAQRKLHEKQMEIEHLKTSGLQDKALYDYTFARDNG
                     INPEIKLAHNYVSNWEEMKANASGLLIWGDVGTGKSFFAGCIANALLEKGVPVLMTNF
                     SRILNTLTGIHFEDRNQFINSLNRYSLLIIDDLGIERNSDFALEQVFNVIDSRYRSKK
                     PLIITTNLTLSELNNAADIAHKRIYDRILERCIPVRINNRNIRQDNATANLKQAKKIL
                     LNNHAPNQN"
     gene            1303..1524
                     /locus_tag="ACZPD3_09760"
     CDS             1303..1524
                     /locus_tag="ACZPD3_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HIR1416304.1"
                     /translation="MADKRIMTPEEKALLQARHRQEEAEARNRKKERDARTHRLVQEG
                     AILESIVPHIKEMDLDSLKRELMIRLRGM"
BASE COUNT          583 a          376 c          353 g          385 t
ORIGIN      
        1 aagagataaa aaagaacaat ttagcaataa gagggagtaa agagccacgc tcaccctatg
       61 gaacatttca aaatgttttt ctgtcagaaa aggagatgga agatatccag cagacaatcc
      121 ctgactggca ggattatatg gaacgcttat ccggttatat ggcttctacc ggaaagcaat
      181 atcagaacca tgcagctacc atcatcagtt gggcaagaca ggatcatcct gcttcccggc
      241 aaaggaatta cgaaagtgag gaatacgaaa cactatgaca agatttacag aaaaagaaaa
      301 tgctataaca ccaaacagag aacttcagcc tgatgaatat tttaacgaaa cagaccacct
      361 gatctactgt tccaaatgca atacgccaag acaatgccgg catgaattac agggaaaggt
      421 tcttattcct tccatccgct gtaaatgcca gcaggaaatc tttgagcagg aagaggccca
      481 gagaaaactt catgaaaaac aaatggaaat agagcatctt aaaaccagcg gcttacagga
      541 caaagcactc tatgactaca cttttgccag ggataacggc atcaatccgg aaataaaact
      601 ggcacacaat tatgtaagca actgggaaga gatgaaagca aacgcttccg gacttctcat
      661 ctggggtgat gtcggtactg gaaaatcttt ttttgcaggc tgcattgcca atgcccttct
      721 ggaaaaaggc gttcctgtac tgatgaccaa cttttcccgg attctgaata cccttaccgg
      781 aatacatttt gaagacagaa accagttcat caacagctta aaccgctaca gtcttctgat
      841 tattgatgat ctcggaattg agcggaactc tgactttgca ctggaacagg tattcaatgt
      901 gatcgacagt cgttaccgca gtaaaaagcc tctgatcata acgaccaatc tcactttatc
      961 cgagctgaat aacgccgctg atatcgcaca caaacgaatt tatgaccgta ttctggagag
     1021 atgtattcct gtccgtatca ataaccggaa tattcgtcag gacaatgcaa cagctaattt
     1081 aaaacaggcg aaaaaaatcc tgttgaacaa tcatgctccg aaccagaatt gactcgaaga
     1141 agtataacac aatgattact tttcactgac agtggtacta ctttctttcc tcatttatta
     1201 ccagaaatat gccactgtca gttatctaaa accatagtac caaaccaaaa gattttaaaa
     1261 ccaaaactgc ccggatacgg acataaaaag gaggtgccag atatggcaga taaaagaatc
     1321 atgacaccgg aagaaaaagc acttttacag gcaagacacc gccaggaaga agccgaagca
     1381 agaaatcgca aaaaagaacg tgatgccaga acacaccgat tagtccagga aggagccatt
     1441 ctggaaagta ttgttcccca tattaaagaa atggatttag attccctgaa acgggagctg
     1501 atgatccggc tacggggaat gtaaacgcac aagttctact cacgaagggc gcacttactc
     1561 accacctgat aaatcaggca gtggtatccc cttcgaggct cgtgcgctct gccgagggct
     1621 ttatccgctg ttgcaggcaa catcgaaagg aggtgccaga tatggcaatt tatcatatgc
     1681 aagccaaagt cgtcagc
//
LOCUS       DBMDXG010000416         1694 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_14097_length_1694_cov_1.035782, whole genome shotgun sequence.
ACCESSION   DBMDXG010000416 DBMDXG010000000
VERSION     DBMDXG010000416.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14097_length_1694_cov_1.035782"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(155..712)
                     /locus_tag="ACZPD3_09765"
     CDS             complement(155..712)
                     /locus_tag="ACZPD3_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR1416305.1"
                     /translation="MGLWELFVLAVGLSMDAFSVAVCKGLSVCQIRKKQILTVGLYFG
                     FFQGFMPLVGYLLGTGFQEKIQSIDHWIAFILLSLIGINMIRESGGEAENLDACFTFK
                     AMLPLAIATSIDALAVGVTFAFLKVQIIPAVSFIGITTFVISCAGVLIGNRFGARYKS
                     KAELAGGVVLILMGIKILLEHTILA"
     gene            complement(959..>1694)
                     /locus_tag="ACZPD3_09770"
     CDS             complement(959..>1694)
                     /locus_tag="ACZPD3_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR1416306.1"
                     /translation="ELEAKVLDIALMLHAEHGGGNNSTFTTHVVTSSGTDTYSSVAAS
                     IGSLKGPRHGGANLKVQDMFADIKAHVKNWTDEAEVEAYLCKILNKEAFDHSGLIYGM
                     GHAVYTLSDPREVILKKFARKLAQEKGMMEEFALYELVERLGSRLVMEQRKMFKNVCA
                     NVDFYSGFVYTMLGIPKELFTPIFAIARIPGWSAHRLEEILNAGKIIRPAYKYVGHHQ
                     EFVAMPDRDPCMEYPCMDEENDKNGD"
BASE COUNT          460 a          435 c          335 g          464 t
ORIGIN      
        1 cccctcagtt cctgaggatc gctttatggt actgaaaacg ccgcagctct gccggtcacc
       61 ctggttacgt acttatctgt aaaacccggt gtccgaaagc agttctgcgg cgttttttct
      121 gtaaaaatta aatatatgtt ctctccagat tatttcatgc cagaatggta tgctccaaca
      181 gtatcttaat tcccataagg atcagaacaa cacctccggc aagttctgct tttgatttat
      241 atctggctcc aaaccggttt ccgatcagga ctcctgcgca ggagatcaca aaagtagtga
      301 ttccgataaa gctgactgcc ggaatgatct gaaccttcag aaacgcaaag gtcactccca
      361 ctgccagcgc atcaatgctt gtagctatgg ccagaggaag cattgcctta aatgtaaagc
      421 aggcatccag attttctgcc tctcctccgg attcccggat catattgata ccgatgaggg
      481 acaaaagaat aaatgcgatc cagtgatcaa tgctctggat tttttcctgg aagccagttc
      541 ccagaagata gcccaccagg ggcataaatc cctgaaaaaa gccaaaatac agtcccactg
      601 tcagaatctg cttttttctg atctgacaga cagaaagtcc tttacagact gccacagaaa
      661 acgcatccat ggaaagtccc actgcaagta caaaaagttc ccataatccc attttatttt
      721 cctccaatgt aagtatttta tcagagaagc agtaagcaca gacgatctgt ttttcttgca
      781 cagggcgcaa aaaacgagac tatccgccct gtgcagataa gtctcgtcat ttcagatggg
      841 gccaggatct actccagaat gttgacctca tcataccatt tttcaaaaat cttatttttc
      901 catatgatac cgactactcc cttatgtcgg ttcagtatac cagaaataat tctgtttttc
      961 aatccccgtt tttatcattt tcctcatcca tacaaggata ctccatgcag ggatctctgt
     1021 ccggcatcgc aacaaactcc tgatgatgtc ccacatattt atatgcagga cggatgatct
     1081 tgccagcgtt aaggatttct tccagacggt gagcgctcca tccgggaatt cgggcaatgg
     1141 caaaaatagg tgtgaacagc tccttgggga tgcccagcat agtatacaca aagccgctgt
     1201 aaaaatccac attggcgcac acatttttaa acattttcct ctgttccata acaagacggc
     1261 ttcccagtcg ctccaccagc tcataaagag caaattcctc catcattccc ttttcctggg
     1321 caagcttgcg ggcaaatttt ttcaggatca cctcacgggg atctgacaga gtatatactg
     1381 catggcccat tccgtagatc agaccggaat ggtcaaaggc ttctttattc aggatcttac
     1441 agaggtatgc ctccacctct gcctcgtctg tccagttttt tacatgagct ttgatatcag
     1501 caaacatatc ctgcaccttc agatttgccc caccgtgtct tggccctttc agggaaccaa
     1561 tagaagctgc cacggaagaa taagtatctg ttcctgatga ggtaacaaca tgggtggtaa
     1621 atgtagagtt gtttccgcct ccgtgctctg catggagcat cagggcaata tccagaacct
     1681 ttgcttccag ctct
//
LOCUS       DBMDXG010000417         1657 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_14506_length_1657_cov_1.987789, whole genome shotgun sequence.
ACCESSION   DBMDXG010000417 DBMDXG010000000
VERSION     DBMDXG010000417.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14506_length_1657_cov_1.987789"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="ACZPD3_09775"
     CDS             complement(<1..298)
                     /locus_tag="ACZPD3_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HIR1416307.1"
                     /translation="MKKSKGIVVLLLTLILTVFFCFTAAVGIGPTGTGAAKHINTGLD
                     LAGGVSITYQAKESNPTSEEMSDTIYKLQKRVEQYSTEAQVYQEGSDRITVEIPG"
     gene            complement(410..>1657)
                     /gene="scfB"
                     /locus_tag="ACZPD3_09780"
     CDS             complement(410..>1657)
                     /gene="scfB"
                     /locus_tag="ACZPD3_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR1416308.1"
                     /translation="LGTEAIAEKLENKYNREDIETSVRECNKLKEDGMLFTKDVYENV
                     IEEFSNNRQTVVKALCLHIAHDCNLACRYCFAEEGEYHGRRALMSYEVGKKALDFLIA
                     NSGSRKNLEVDFFGGEPLMNWQVVKDLVKYGREQEKLHNKKFRFTLTTNGVLLNDEVM
                     EFCNKEMGNVVLSVDGRKEVHDYMRPFRKGAGSYDLIMPKFQKFAESRNQDKYYVRGT
                     FTHHNLDFSKDVLHLADLGFKQISVEPVVAADTEEYAIREEDIPQIMEEYDTLAKEMI
                     KREKEGKGFNFFHFMIDLTGGPCVYKRLSGCGSGTEYLAVTPWGDFYPCHQFVGEEQY
                     LMGNVDEGITKPEIQKEFGGCNVYTKKDCKDCFARFYCSGGCAANAFHFQGDIKGNYE
                     IGCELQRKRVECAIMIKAALACD"
BASE COUNT          401 a          420 c          310 g          526 t
ORIGIN      
        1 caccaggaat ttcaactgta atacggtctg atccttcctg gtatacctga gcctcagtac
       61 tgtactgctc tacacgcttc tgaagcttgt agatcgtatc tgacatctct tcgcttgtcg
      121 gattgctctc cttagcctgg taggtaatgc tgacaccacc ggcaagatca agacccgtgt
      181 tgatatgttt tgctgcaccg gttccggtag gaccgatacc tacggctgca gtaaagcaaa
      241 agaacacagt caggatcagt gtcagaagca gcactacgat tcctttactt ttcttcatag
      301 ttcttttccc ttcttgattt tttgtccgcg gtcatgttca tccgcctgcc agccattttc
      361 tgttatgata acagccgacg gcggtatcag tcagacagat tactgcccac taatcacatg
      421 ccagtgcggc ttttatcatg atggcgcact ccacacgttt tctctggagt tcgcatccga
      481 tttcataatt gcctttgata tctccctgga aatggaatgc atttgccgca catccgccac
      541 tacagtagaa acgtgcgaag cagtctttgc agtctttctt tgtgtaaacg ttgcatccgc
      601 cgaattcctt ctggatttcc ggctttgtga tgccttcgtc tacatttccc atgagatact
      661 gttcttcacc aacaaactgg tgacatgggt aaaaatcacc ccagggtgtt acagccaggt
      721 actctgttcc tgaaccgcag cctgacagtc tcttgtatac acaagggcca cctgtaaggt
      781 cgatcataaa atggaagaag ttaaatcctt ttccctcttt ctctctttta atcatttctt
      841 ttgcaagagt atcatactct tccatgatct gggggatatc ctcctcgcgg atcgcatatt
      901 cctccgtatc agccgcaact acaggctcta cagaaatctg tttgaatcca agatctgcca
      961 gatgaagcac atccttggag aaatcaagat tatggtgggt aaaagtacct cttacataat
     1021 acttgtcctg atttctggat tctgcaaatt tctggaactt cggcatgatc aggtcatagc
     1081 ttcctgcacc ttttctgaat ggacgcatgt aatcatgtac ttccttacgt ccgtccacac
     1141 tcagtacaac gttgcccatc tctttattgc agaattccat tacttcatca ttcagaagaa
     1201 ctccgtttgt tgttaaagtg aaacggaatt tcttattgtg aagcttttcc tgctctctgc
     1261 catattttac cagatctttt acaacctgcc agttcatcag cggctcgccg ccaaagaaat
     1321 ctacttccag gtttttcctg gaaccggagt tcgcgatcag gaaatcaaga gcttttttgc
     1381 cgacttcata agacataaga gctcttctgc catgatattc gccttcttcg gcaaaacaat
     1441 aacggcaggc cagattgcag tcatgagcaa tgtgcaggca caatgctttt acaacggtct
     1501 gccggttatt tgaaaattct tcaattacat tctcataaac atcttttgta aaaagcattc
     1561 catcttcttt cagcttatta cattctctta cagatgtctc aatatcttct ctgttatatt
     1621 tgttttccag tttctctgca atagcttctg tgccaag
//
LOCUS       DBMDXG010000418         1655 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_14533_length_1655_cov_1.463964, whole genome shotgun sequence.
ACCESSION   DBMDXG010000418 DBMDXG010000000
VERSION     DBMDXG010000418.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14533_length_1655_cov_1.463964"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     regulatory      60..153
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            333..1148
                     /locus_tag="ACZPD3_09785"
     CDS             333..1148
                     /locus_tag="ACZPD3_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR1416309.1"
                     /translation="MKGKRVIAGILLVGILATALAGCKNADVTKKEREKPVITLGSDS
                     YPPYNYLNEDGIPTGIDVELATEAFRRMGYQVDVVQINWEKKKELVESGEIDCIMGCF
                     SMEGRLDDYRWAGPYIASRQVVAVNESSDIYKLSDLEGKNLAVQSTTKPEGIFLNRTD
                     ERIPKLGNLISLGHRELIYTFLGKGYVDAVAAHEESIVQYMKDYDASFRILEDPLMVV
                     GIGVAFAKEDDRGICEQMDQTLEEMRQDGTSLKIIKKYLDDPQKYLEVDDLGY"
     gene            1132..>1655
                     /locus_tag="ACZPD3_09790"
     CDS             1132..>1655
                     /locus_tag="ACZPD3_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR1416310.1"
                     /translation="MILDIRRRIKVKRFWIFGILLGICVTAASLYYFFQAEKKEAENR
                     MVKTVNYVKVQCSTYTHYNEASESKSLLRAIESARQMSTNIDMETENGGRLSQEFLKE
                     NLQTLWVDGILVLDAEGKTVCKYSMDEALTNEITDYLQKDIIMDFTGYEERSYSERID
                     REDGSRIDIAACAR"
BASE COUNT          539 a          294 c          425 g          397 t
ORIGIN      
        1 aacatatttt cttatagaca tgatatagta tgagtgctat aataaaaata attttttatg
       61 gcatttattg gcaaaccggt tgaaaagccg gggcgcaaag ccagaaggag ctaaagtcag
      121 attgaaatct ggccatgcca gccggttgca tcatgcctca gtaataaaaa aaggtaaatg
      181 ttaaatgggc attatggaaa aagttcctgt aaaatttttt acaaggaact tttttttgtc
      241 ttcaagcgga tattcgcaac ctccgcaggg gacttacctg attcggttaa atttgatgga
      301 aaatagagaa gaacagcagg aatggagaaa agatgaaagg aaaaagagtg attgcaggca
      361 ttctgcttgt gggaatttta gcaactgccc tggcagggtg caaaaatgca gatgttacta
      421 aaaaagaaag agaaaagccc gttatcaccc tgggcagtga cagttatccg ccatacaatt
      481 atctgaatga agatggaatc cccacaggaa ttgatgtgga actggctaca gaagctttca
      541 gaagaatggg gtatcaggtg gatgttgtcc agatcaactg ggagaagaaa aaagaactgg
      601 tagaaagcgg agagatcgac tgtattatgg gctgtttttc catggaagga cgccttgacg
      661 attaccgctg ggcaggaccc tacatagcaa gccgccaggt agttgctgta aatgagagca
      721 gtgatatcta taaactaagt gatctggagg gaaagaacct tgccgtccag tccacaacca
      781 aaccggaagg catctttctg aaccggacag atgaaaggat ccccaaactg ggcaatctga
      841 tcagtcttgg gcacagggaa ctaatctata cctttctggg aaaaggatat gtagatgcag
      901 ttgctgcaca tgaggaatcc attgtccagt acatgaagga ttatgatgca agcttccgta
      961 tcctggaaga tccgctgatg gtggtcggga taggtgttgc ttttgcaaaa gaggatgaca
     1021 gaggaatttg tgagcagatg gatcagacgc tggaagaaat gcgtcaggat ggcacatccc
     1081 tgaaaatcat taaaaaatat ctggatgatc cccagaagta tctggaggtg gatgatcttg
     1141 gatattagga gaagaataaa agtaaagcga ttctggatat tcggcattct gcttggaatc
     1201 tgtgtgactg cagcttccct gtattacttt tttcaggcag agaaaaagga agcagagaac
     1261 aggatggtga aaactgtaaa ctatgtcaaa gtgcagtgtt ccacctacac ccattataat
     1321 gaagcttcgg aatctaaaag tctcctccgt gccattgaaa gcgccagaca gatgagtacg
     1381 aatatcgaca tggaaacaga aaacggcggc cggctaagcc aggagtttct gaaagaaaat
     1441 ctgcagaccc tctgggtgga tggtatcctt gtgttggatg cagagggaaa gacagtctgc
     1501 aaatacagca tggatgaggc tctgaccaat gagatcacag attatctgca aaaagacatt
     1561 atcatggatt ttaccggata tgaagaaaga tcttattcgg aacggattga ccgagaagac
     1621 ggatcacgta tcgacattgc agcctgtgcc agaaa
//
LOCUS       DBMDXG010000419         1637 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_14729_length_1637_cov_1.090495, whole genome shotgun sequence.
ACCESSION   DBMDXG010000419 DBMDXG010000000
VERSION     DBMDXG010000419.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14729_length_1637_cov_1.090495"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            100..1116
                     /gene="galE"
                     /locus_tag="ACZPD3_09795"
     CDS             100..1116
                     /gene="galE"
                     /locus_tag="ACZPD3_09795"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR1416311.1"
                     /translation="MAILVTGGAGYIGSHTVVELQNAGYDVVVLDNLSNASEKSLERV
                     SKITGKPVKFYKADILDRDALNEVFDKEDIDSCIHFAGLKAVGESVAKPWEYYENNIA
                     GTLTLVDVMRKHNVKNIIFSSSATVYGDPAIIPITEECPKGQCTNPYGWTKSMLEQIL
                     TDIQKADPEWNVVLLRYFNPIGAHKSGTIGENPNGIPNNLMPYITQVAVGKLKELGVF
                     GNDYDTPDGTGVRDYIHVVDLAKGHVKALKKIEDNSGLSIYNLGTGKGYSVLDIVKNF
                     EAATGVKIPYVIKARRPGDIATCYSDASKAEKELGWKAENGIKEMCADSWRWQSNNPN
                     GYDE"
     gene            complement(1199..1423)
                     /locus_tag="ACZPD3_09800"
     CDS             complement(1199..1423)
                     /locus_tag="ACZPD3_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR1416312.1"
                     /translation="MSDIKERIIAAVTIMSEKEAEKVWNLIQSAFFLSDVEEVKPEPD
                     ELEALRQYEAGNPDYQPCISTEELKKELNL"
     gene            complement(1429..>1637)
                     /locus_tag="ACZPD3_09805"
     CDS             complement(1429..>1637)
                     /locus_tag="ACZPD3_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR1416313.1"
                     /translation="FIQKWDKPTQLRLFTAIDKLPLEGDIQKLQGTSGFRLRVGNYRV
                     IFDVNGLIVDIIDVGNRGQIYKGV"
BASE COUNT          541 a          301 c          342 g          453 t
ORIGIN      
        1 ctgatactgt gaactaatat aggtattgtt gaaagagaaa aaaacagaaa ttgcaaaaaa
       61 caggtattgt agcaggaaga caggttagga ggaattatta tggcaatttt agttacaggt
      121 ggagcaggct atattggaag ccatacagtt gtagaattgc agaacgcagg atatgatgtt
      181 gtagttctgg ataacttaag caatgcaagt gagaaatctc tggaaagagt ttccaagatc
      241 acaggcaaac cagtgaaatt ctacaaagca gacatcctgg accgcgatgc tttaaatgaa
      301 gtttttgata aagaagatat tgattcctgt attcatttcg caggtttaaa ggcagtagga
      361 gaatctgttg caaagccatg ggaatactac gagaataata ttgcaggaac cttaacactg
      421 gtagatgtta tgagaaaaca taatgtaaag aacattatct tctcctcatc tgcaactgta
      481 tacggagacc cggcaattat cccgatcaca gaagagtgcc cgaaaggaca gtgcacaaat
      541 ccatatggat ggacaaaatc catgcttgag cagatcctga cagacattca gaaagcagat
      601 ccggaatgga acgtagttct tctccgttac tttaacccga tcggtgctca caagagcgga
      661 actatcggag agaatccgaa cggaatcccg aacaacctga tgccatatat cacacaggtt
      721 gcagtaggta aactgaaaga acttggggta ttcggaaatg attatgatac tccggacgga
      781 actggtgtga gagattacat tcacgtagtt gaccttgcaa aaggacatgt aaaagctctt
      841 aagaaaatcg aagacaactc cggtttaagc atttacaatc ttggtacagg aaagggatac
      901 agcgttcttg atatcgtgaa gaactttgaa gctgctacag gtgttaagat tccatatgtg
      961 atcaaagcac gtcgcccggg tgatatcgca acatgctatt ccgacgcttc caaagcagaa
     1021 aaagaacttg gctggaaagc agaaaacgga atcaaagaaa tgtgcgcaga ttcctggaga
     1081 tggcagtcca ataacccgaa tggttatgat gaataatcaa taatacaaag tataaacagc
     1141 ccccagacat ggaaaaaagt acatgtctga gggctgttat aacttttctg ttagcagatt
     1201 ataaattcaa ttcttttttt agttcttcgg tagaaatgca tggttgataa tcaggattgc
     1261 cagcttcata ttggcgtaat gcttctaatt cgtcaggttc aggtttgact tcttccacat
     1321 cagataagaa aaatgctgac tgaattaaat tccatacttt ttccgcctct ttttcactca
     1381 taattgtgac ggcagcaata attctttctt ttatatcaga catattactc acactccttt
     1441 atatatttgt ccgcgatttc ctacgtctat gatgtcgaca atgagaccat taacatcaaa
     1501 tattactctg taatttccaa cacgaagacg aaaaccggag gtaccctgta atttttgaat
     1561 atccccctct aatggtaatt tatcaatagc tgtaaatagt ctcagttgtg ttggtttatc
     1621 ccacttttgt atgaatt
//
LOCUS       DBMDXG010000420         1595 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_15191_length_1595_cov_1.528112, whole genome shotgun sequence.
ACCESSION   DBMDXG010000420 DBMDXG010000000
VERSION     DBMDXG010000420.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15191_length_1595_cov_1.528112"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="ACZPD3_09810"
     CDS             <1..1584
                     /locus_tag="ACZPD3_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR1416314.1"
                     /translation="EISTRFGEKTYRISMQRVSLGEVVAKSEFLENSNRNVSLIAMYL
                     YDDTELKSYIKKNEDNKLVVALAYLDNYEEALESVEDVRRSLLIALIDRKITKYFSNF
                     DGLVKKLEKDKYFLIMRQSSLEALKEQRFHILDEVKTVNIGNEMAITLSIGVGLNAST
                     YIQNYEYSRIAIEMALGRGGDQVVIKNGNNITYYGGKTQQMEKNTRVKARVKAQALKE
                     FMSTKDRVVVMGHKITDVDALGAAIGIFRAGKTLGKSVSIVVNDPTKSIRPLIAGYVN
                     NPDYEPSMFVDSEQAKDMVDNNTVVVVVDTNRPSYTECEELLHMTKTIVVLDHHRRGS
                     EVIENAVLSYVEPYASSACEMVAEILQYFSDDLRIRNMEADCLYAGIMIDTNNFTTRA
                     GVRTFEAAAFLRRSGADVTRVRKLLRDDLKSYQARAEAVRTAQIYREYYAIARCPSEN
                     LDSPTVIGAQAANELLNIAGVKASFVLTQYNNEVYISARAIDEINVQVMMEKMGGGGH
                     MNIAGAQVKPLQPDYGFGH"
BASE COUNT          488 a          274 c          416 g          417 t
ORIGIN      
        1 gagatcagta ctcgatttgg agagaagact tatcgtattt ccatgcagag ggtgtctctt
       61 ggtgaagtgg ttgccaaaag tgagtttctg gagaattcaa acaggaatgt cagcctgatt
      121 gccatgtatc tgtatgacga tacagaactg aagtcttaca ttaaaaagaa tgaagacaat
      181 aagcttgttg tagcgttggc ttatctggat aactatgagg aagcacttga gagtgtagag
      241 gatgtgagac gttcacttct gattgctctg attgaccgga agattacgaa atatttttcc
      301 aatttcgatg gtcttgtaaa gaaacttgag aaggacaaat atttcctgat catgcgccag
      361 agttcgcttg aggctctgaa agaacagaga tttcatattc tggacgaggt taaaacagtt
      421 aatatcggaa atgagatggc gatcactttg agtattggtg tgggactgaa tgcttccact
      481 tatattcaaa attatgagta ttcacgtatt gctatagaga tggcacttgg acgtggcggt
      541 gatcaggttg ttattaagaa tggaaataat attacctatt acggcggaaa gactcagcag
      601 atggagaaga atacccgcgt aaaagccagg gtaaaagcgc aggcactgaa agagtttatg
      661 agtacgaaag acagagtcgt tgtcatggga cataaaatca cagatgtgga tgcgttggga
      721 gcagcaatcg gtattttccg tgcaggtaaa acacttggaa aatctgtcag tattgtagtg
      781 aatgatccga caaaatctat cagaccgttg atcgcaggat atgtgaataa tccagactac
      841 gagccatcta tgtttgtgga cagcgagcag gctaaagata tggtagataa caatacagtg
      901 gtggttgtag ttgatactaa cagaccaagc tatacggaat gcgaggagct tctgcacatg
      961 acaaagacaa ttgttgtgct ggatcatcac aggcggggaa gcgaagtgat agagaatgcg
     1021 gtgctttcct atgtggagcc ttatgcatcc tcagcctgtg agatggtagc agagatcctg
     1081 cagtatttct cggatgacct gcgtatccgc aatatggagg ctgactgtct ctatgcggga
     1141 attatgatcg atacaaacaa ttttactaca agagcaggtg tacgtacctt tgaggctgct
     1201 gcattcttaa gaaggagtgg agcagacgtg accagagtac gtaaactgtt aagggatgat
     1261 ctgaaatctt atcaggcacg tgcagaagca gtgagaactg cacagattta cagagaatac
     1321 tatgccattg ccagatgtcc aagcgaaaat ctggacagcc ctacagtcat aggagcacag
     1381 gcagccaatg aacttctgaa tattgcaggg gttaaggcat cctttgtact gactcagtac
     1441 aataatgaag tttatatcag tgccagagct attgatgaaa ttaatgtaca ggtaatgatg
     1501 gaaaagatgg gcggaggcgg tcatatgaat attgccggtg cccaggtcaa gcctctccaa
     1561 ccggattacg ggttcggtca ttaagcatat tgacc
//
LOCUS       DBMDXG010000421         1561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_15626_length_1561_cov_1.753425, whole genome shotgun sequence.
ACCESSION   DBMDXG010000421 DBMDXG010000000
VERSION     DBMDXG010000421.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15626_length_1561_cov_1.753425"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(93..1274)
                     /locus_tag="ACZPD3_09815"
     CDS             complement(93..1274)
                     /locus_tag="ACZPD3_09815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR1416315.1"
                     /translation="MKKDGYYSSGEFARMAHVTLRTIRYYDKQNILKPSYVTESGARF
                     YTDEDFARLSQILLLKYLGFSLDDIREMTIDDSDYHFMENSLNIQLKLVRDRIEQMQL
                     VEKAIQDTTDAIRSHHAIDWNQMLDLIHLTGMEKSMKNQYQNASNISSRINLHSLYSQ
                     NKQGWFPWVYEQCQIHPGMRILELGCGDGVLWTQNISSLPGKVSVTLSDLSSGMLRDA
                     RRAIGRKDSRFSFEAFDCARIPHEDRSFDLVIANHVLFYCEDISAVCSEIQRVLTPGG
                     KLICSTYGKKHMQEVSRLVRDFDDRIVLSADKLYERFGRENGADILAPYFSRIFWESY
                     KDSLLVPDAEPLISYILSCHGNQNQYLLDKYKDFRGFVSRKTKDGFFITKDAGIFLCE
                     K"
BASE COUNT          432 a          330 c          362 g          437 t
ORIGIN      
        1 agttacgata tcatcctgac tctggtgatt gtattataaa aggtgacctt aggtcacctt
       61 caagtgcttt ttacaaaaaa gttaaatttt ggttattttt cacaaagaaa aatgccggca
      121 tccttggtaa taaagaaccc atcctttgtc tttcgcgata caaatcctcg gaaatctttg
      181 tatttgtcca gaagatactg attctgattt ccatggcagg agaggatata ggaaatcagc
      241 ggttctgcat cggggacaag aagagagtcc ttatagcttt cccagaaaat acgggaaaaa
      301 tacggtgcca ggatatctgc tccgttttct ctgccaaaac gctcatagag tttgtctgcg
      361 gagagaacga tccggtcgtc aaaatcccgg accagtctgc tgacttcctg catgtgtttt
      421 tttccatagg tactgcagat cagttttccg cccggagtga gcacacgttg aatttcgctg
      481 catacagcag agatatcttc acagtaaaaa agcacatggt ttgcaatgac cagatcaaag
      541 cttctgtctt catgaggaat tcttgcacag tcaaatgcct cgaaggaaaa acggctgtct
      601 ttgcgtccga tagcacgtct ggcatcacga agcattccgg atgaaagatc agaaagagta
      661 acggaaactt taccggggag gctggaaata ttctgtgtcc acagaacacc gtcgccgcat
      721 ccaagttcaa ggatacgcat ccccggatga atctggcact gctcataaac ccatggaaac
      781 catccctgtt tattctggga ataaaggctg tgcagattaa tgcgtgagga aatgttggag
      841 gcgttctggt attgattttt catgcttttc tccatgcctg tcaggtggat caggtcaagc
      901 atctgattcc agtctatggc atgatgggag cggatcgcat ctgtggtatc ctggattgcc
      961 ttttccacca gctgcatctg ctcgatacgg tcacgtacca gctttaactg gatgttcagg
     1021 gaattttcca taaaatgata gtctgagtcg tcaatggtca tttcccgtat gtcatccagg
     1081 gagaaaccca gatatttcag aagcaggatc tgggaaagtc ttgcaaaatc ctcatctgta
     1141 taaaaacgtg cgccggattc tgttacatag gagggtttga ggatattctg tttgtcataa
     1201 tagcggatag tgcggagtgt tacatgtgcc atccgtgcaa attctccgga agagtagtag
     1261 ccgtcttttt tcattgtaaa accttcttta agaaaaatag tcttgcccgc aaatgtggat
     1321 agaaacagtt gttcagcctg catttgcgta ttgctgatat gtgtagagta taacacagct
     1381 gaccggcagg ctacaagcat caggtattac cggatggata atatactgtc tgaatgtacc
     1441 actatcccgc gaggtgtttt ggcatcaata tgccaaaatc ccgagacata caagccaaaa
     1501 ttccattgcc gcaggcaatc tggaatgaat tttggctaag ttactgtgaa cggagtgaac
     1561 a
//
LOCUS       DBMDXG010000422         1545 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_15820_length_1545_cov_1.172438, whole genome shotgun sequence.
ACCESSION   DBMDXG010000422 DBMDXG010000000
VERSION     DBMDXG010000422.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15820_length_1545_cov_1.172438"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="ACZPD3_09820"
     CDS             complement(<1..177)
                     /locus_tag="ACZPD3_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR1416316.1"
                     /translation="MPTYNIKRNGWKNLHKNTRSTGSCYERKAADYLKQQGLFILRYN
                     YRCRFGEIDLIARDG"
     gene            complement(248..1003)
                     /locus_tag="ACZPD3_09825"
     CDS             complement(248..1003)
                     /locus_tag="ACZPD3_09825"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR1416317.1"
                     /translation="MKTIAEIKAEFAAADMSSYPALYEIYKEDTRSGVQKLIAQCRKK
                     EAALEAEKQRIENMKVYEHKYEHLGYLCGIDEVGRGPLAGPVVACAVILPQDSRILYL
                     NDSKKLSASKREELYDVIMREAVSVGIGMRSPERIDEINILQATYEAMREAVSKLEVV
                     PQLLLNDAVTIPQITIPQVPIIKGDAKSVSIAAASIVAKVTRDRMMEEYDKVFPEYGF
                     ASNKGYGSADHIAALKKYGETPIHRKTFITHFI"
     gene            complement(1000..>1545)
                     /gene="ylqF"
                     /locus_tag="ACZPD3_09830"
     CDS             complement(1000..>1545)
                     /gene="ylqF"
                     /locus_tag="ACZPD3_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR1416318.1"
                     /translation="QEACKEKIERDRRRGIKNRPIRAMVVGIPNVGKSTFINTFAGRA
                     CAKTGNKPGVTKGKQWIRLNKGVELLDTPGILWPKFEDQQVGIRLACVGSIKDDILNM
                     EELALWLIDYLRENYKGILAERYQITEEGTSVEVLEAIARARGCLKKQEELDYAKASL
                     ILFDDFRSGKMGRITLEWAPL"
BASE COUNT          365 a          404 c          275 g          501 t
ORIGIN      
        1 tccatctctg gcaatcagat cgatttctcc aaatctgcat ctgtaattat atctcagaat
       61 aaataatccc tgctgtttca gataatcagc agcttttcgt tcgtaacaac ttccggtaga
      121 ccgggtattt ttatgcaaat tcttccatcc atttcttttt atattatatg taggcatctt
      181 ttgttgttac tgtgcatcac tcagtgtatt gctgtgtact gaaggatagt aacccttttg
      241 cgactgttta tatgaaatgt gtaataaagg tctttctgtg gatcggggtt tctccgtatt
      301 ttttcagtgc cgcaatatga tcggcagaac catatccctt atttgatgca aaaccatatt
      361 ccgggaacac tttatcatat tcttccatca ttctgtccct tgtcactttt gcaacaatac
      421 ttgctgctgc aatggaaaca ctctttgcat ctcccttaat aatgggaacc tgtggaatgg
      481 tgatctgagg aatcgtgact gcatcattga gaagaagctg aggaaccact tccagtttac
      541 ttaccgcctc ccgcattgcc tcataagtag cctgaaggat attgatctcg tcaatacgct
      601 ccgggctgcg cataccgatt cctactgaaa ccgcttccct cataatcaca tcatacagtt
      661 cttctctttt ggaagcactg agtttcttgg aatcgttcag ataaaggatc cggctgtcct
      721 gtggcagaat tactgcacat gccacaaccg ggcctgcaag ggggcctctt cccacttcat
      781 caattccgca taaataacca agatgttcat atttatgctc atataccttc atattttcaa
      841 tacgctgctt ctccgcttcc agagctgctt cctttttgcg gcactgtgcg atcagctttt
      901 gcacaccact tcttgtatct tctttataaa tttcatataa agccgggtag ctgctcatat
      961 ctgctgcagc aaactcggct ttaatctcag ctattgtttt cataatggcg cccactccaa
     1021 tgtgatccgc cccatttttc cggaacggaa atcatcaaac agtatcagcg aagctttggc
     1081 atagtccagc tcttcctgct ttttcagaca ccctctggct ctggcaatcg cctccagaac
     1141 ttccacagaa gttccctcct ctgtgatctg atatctctct gccagaatcc ctttataatt
     1201 ttcgcgcaga taatcgatca gccagagtgc cagttcctcc atattcagaa tgtcatcttt
     1261 gatagaacct acacatgcca aacggattcc cacctgctgg tcttcaaatt tcggccacaa
     1321 aataccagga gtgtccaaaa gttctactcc cttattcaga cggatccact gttttccttt
     1381 cgtaactccc ggcttgtttc cggtctttgc acaggcccgt cctgcaaagg tattgataaa
     1441 tgtagatttt cctacattcg ggattcccac taccatagcg cggatcggac ggtttttaat
     1501 accgcggcgt ctgtctcttt ctatcttttc cttacatgct tcctg
//
LOCUS       DBMDXG010000423         1544 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_15830_length_1544_cov_1.020097, whole genome shotgun sequence.
ACCESSION   DBMDXG010000423 DBMDXG010000000
VERSION     DBMDXG010000423.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15830_length_1544_cov_1.020097"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /gene="spoIVA"
                     /locus_tag="ACZPD3_09835"
     CDS             complement(<1..1347)
                     /gene="spoIVA"
                     /locus_tag="ACZPD3_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR1416319.1"
                     /translation="MENFQVYRDIQARTGGDIYIGVVGPVRTGKSTFIRRFMELVALP
                     QMSDTKQAEIRDQLPLSGSGKIITTAETKFIPKEAVPITLGEDQQVKIRLIDSVGFLV
                     KGASGQTEDGKERMVKTPWFEQAIPFREAARIGTQKVIQEHSTIGIVVTTDGSFGELP
                     RDNFSEAEEKAIQELKKQQKPFIVLVNSQMPYKDAALKTAEEIQQKYKVTALTVNCDQ
                     LRKEDIARILEKVLYEFPVSQIQFFIPRWVEMLPLEHELKQQILSQIRDKMKSMQHIR
                     DITKESVKLSGPYVQDSLLEDVGLSDGTVKVRIRIKEEYYYRMLSQMSGIEMESEYEL
                     IHTMQELVHMKEEYVKVQAALEAVRGTGYGVVVPNLDEIEIAQPEVIRQGNKYGVKIK
                     SKSPSIHMIKANIETEIAPIVGTEQQAKDLIEREHYDRDKVLVVFLPDCHESLAGIV"
BASE COUNT          374 a          385 c          276 g          509 t
ORIGIN      
        1 aacaattccc gccaggcttt catgacagtc cgggagaaaa actaccagaa ccttatccct
       61 gtcatagtgc tcccgttcaa tcagatcctt tgcctgctgt tctgttccta ctatgggagc
      121 aatttcagtt tctatatttg cctttatcat atggatagac ggactctttg acttgatctt
      181 tactccatat ttattcccct gtcggatcac ttcaggctgt gcgatctcaa tctcatccag
      241 attcgggaca accacaccat aaccggttcc acgcactgct tccagtgcag cctgcacttt
      301 tacatattcc tctttcatat gtaccagttc ctgcattgta tggatcagtt catattcact
      361 ctccatttcg attccactca tctgactgag catcctgtaa taatattctt cctttatgcg
      421 tattcttacc tttactgttc catcggataa gcctacatct tccagaaggg aatcctgtac
      481 ataaggtccg gataatttta cagattcctt tgtgatatca cgtatatgct gcatactttt
      541 cattttatcc ctgatctgtg aaaggatctg ctgttttaat tcatgctcca ggggaagcat
      601 ttctacccag cgcggaataa aaaactgaat ctgactgaca ggaaattcat ataaaacctt
      661 ctccagaatc ctggcaatat cttcttttct caattggtca cagtttactg tcagagcagt
      721 taccttatat ttctgttgga tttcttccgc tgttttcaga gccgcatctt tatatggcat
      781 ctgcgaattt accagcacga taaatggttt ctgctgtttt ttcagttcct gaatagcctt
      841 ctcttctgcc tccgaaaaat tgtctctggg aagttcaccg aaacttccgt cagttgtgac
      901 cacaatacct attgtggaat gctcctgaat tactttctgt gttccgatgc gtgcggcctc
      961 gcgaaatggg attgcctgct caaaccaggg agtttttacc attcgttctt tcccgtcctc
     1021 cgtctgcccg gatgcaccct ttaccagaaa acccacactg tcgatcaggc ggatctttac
     1081 ctgctgatct tctccgagag tgatgggaac tgcttctttg ggaataaatt ttgtttctgc
     1141 tgtggtgatg attttgcctg atccgcttaa aggaagctga tctctgatct ctgcctgctt
     1201 tgtatctgac atctgtggca atgcaacaag ttccatgaat ctgcggataa atgtagattt
     1261 tcctgtgcgg acaggtccta cgacccctat gtatatatct ccccctgtac gggcctggat
     1321 atcacgatac acctgaaaat tttccataaa aatagccccc ttgcatattt acacataacc
     1381 ttcatgtatt gttataaaat atgcaaggaa gctttaaaat attctattta tcatttggac
     1441 atattgttac tgttcacaca ccactatccc gcgaggtgtt ttggcatgaa tatgccaaaa
     1501 tcccgagaca tacaagccaa aattctattg ccgcaggcaa tctg
//
LOCUS       DBMDXG010000424         1534 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_15913_length_1534_cov_2.159107, whole genome shotgun sequence.
ACCESSION   DBMDXG010000424 DBMDXG010000000
VERSION     DBMDXG010000424.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15913_length_1534_cov_2.159107"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="ACZPD3_09840"
     CDS             <1..1480
                     /locus_tag="ACZPD3_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Helicase associated domain protein"
                     /protein_id="HIR1416320.1"
                     /translation="ENQKLLEQLRRALEQAEGLDIVFKKHMEKKNGKYIVFCSGKEHM
                     DEMKEQTSTWFRRVDGEPRIYTAFYNDTATDREFREFKKDNSAHLKLLFCIDMLNEGV
                     HVDDVDGVILLRPTVSPIIYLQQIGRALSAGSRKTPVIFDLVNNFDSLYCIDSLKKEL
                     EEAFVMFPTTYGEGTHFSGRFQITDEIKDCRVLFQKLQANLSSAWETYYIAAKQWYQE
                     NGNLRVPKNYVTASGLTLGSWIQTQRRVYTGTVTGNLTEEKVRKLNEISMIWDVRDHG
                     WNEALTEMQAYYKEHGNLDIRARYETADGFKLGRWICNLRTKVKTKGLDQALTKEQQE
                     QLAALGMIWDRNTEKWEEYFEAAQEYYRQHGNLDVMAKYVTEEGLPLGRWLSDISNKV
                     SDENLRRVSLTDEQLERLKSIGFKQEKKTARQWNEKYELAKNYYEIHGDLNVPLSYCV
                     NDVKLGRWISNIRSKRKHPGSNGMALSQDRIRQLDNIGMNWK"
BASE COUNT          518 a          282 c          412 g          322 t
ORIGIN      
        1 agagaaccag aaactgcttg agcagctgcg ccgcgcactg gaacaggcag aagggctgga
       61 cattgtgttt aagaagcaca tggaaaagaa aaatggaaaa tatatcgttt tttgttcagg
      121 caaagaacat atggacgaaa tgaaagagca gaccagtacg tggtttcgcc gggtagacgg
      181 cgagccgcgg atctatacag cgttttataa cgatactgct acagacagag aattcaggga
      241 atttaagaag gataacagtg cacatctgaa gctccttttc tgcattgata tgctcaatga
      301 aggggttcat gtggatgatg tggacggtgt gattcttctg cgtccgacgg tgtcgccgat
      361 tatttacctt cagcagatcg gacgtgcatt gtcagcgggc agcaggaaga caccggttat
      421 ttttgacttg gttaataatt ttgacagcct ctactgcatc gactccctga aaaaagaact
      481 ggaagaggca tttgtaatgt ttcccacaac ctacggggaa ggaacgcatt tcagcggtcg
      541 ctttcagatc acagatgaga taaaggactg ccgggttctc tttcagaagc tacaggccaa
      601 cttaagctct gcatgggaaa cctactatat tgccgcaaag cagtggtatc aggaaaacgg
      661 aaacttgaga gtcccaaaga actatgtgac tgcctcggga cttacactgg gaagttggat
      721 acagacccag cgaagagtct atacaggcac ggtgacagga aatctgacag aagaaaaagt
      781 aaggaaactg aacgagatca gcatgatctg ggatgtcaga gaccatggct ggaatgaggc
      841 gctgacagaa atgcaggcat actataaaga acatggaaat ctggatatca gagcaagata
      901 tgagacagct gacggcttta agcttggcag atggatttgc aatctccgga caaaagtaaa
      961 aacaaaaggg ctggatcagg cactgacaaa ggagcagcag gaacagcttg cagcactggg
     1021 catgatctgg gacagaaata cggagaagtg ggaagaatac ttcgaagcag cacaggaata
     1081 ttacagacag catggaaatc tggatgttat ggcgaaatat gtcacagaag aaggtctccc
     1141 tcttggcaga tggctgtcag atatcagcaa caaggtatca gatgaaaatt tacgccgtgt
     1201 atcactcaca gatgaacagc tggagcgtct taagagcata ggctttaagc aggaaaaaaa
     1261 gacagccaga cagtggaatg agaaatatga gctggcaaag aattattacg aaatacatgg
     1321 agatctgaat gttccgctgt cgtactgcgt aaacgacgtg aagctgggaa ggtggatatc
     1381 caatataagg tccaaacgga aacatccggg atccaacgga atggcattaa gccaggacag
     1441 gatcagacag ctggacaata taggtatgaa ctggaaatag gaagagaaaa agaaaattat
     1501 ttacaggagt taggtatgta cagaaaaaaa agaa
//
LOCUS       DBMDXG010000425         1519 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Blautia wexlerae isolate MAG1184
            NODE_16102_length_1519_cov_0.890691, whole genome shotgun sequence.
ACCESSION   DBMDXG010000425 DBMDXG010000000
VERSION     DBMDXG010000425.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508283
            Sequence Read Archive: ERR10114000
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Blautia wexlerae (human gut metagenome)
  ORGANISM  Blautia wexlerae
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Blautia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.7476x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 17:15:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,970
            CDSs (total)                      :: 1,942
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 28
            tRNAs                             :: 25
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 9 of 53
            Pseudo Genes (incomplete)         :: 37 of 53
            Pseudo Genes (internal stop)      :: 9 of 53
            Pseudo Genes (multiple problems)  :: 2 of 53
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Blautia wexlerae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16102_length_1519_cov_0.890691"
                     /isolate="MAG1184"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:418240"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="ACZPD3_09845"
     CDS             complement(<1..312)
                     /locus_tag="ACZPD3_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HIR1416321.1"
                     /translation="MIIQESMTLKRKLEILSDAAKYDVACTSSGVDRKGDGKGMGNSI
                     AAGICHSFAQDGRCISLLKILYTNECVFDCKYCINRASNDVVRTSFTPEEVCRLTMEF
                     YR"
     gene            complement(660..>1519)
                     /locus_tag="ACZPD3_09850"
     CDS             complement(660..>1519)
                     /locus_tag="ACZPD3_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR1416322.1"
                     /translation="FELGGQKVYVHEGGKYAVLEDGTVTTFSRGGSDVTGSLVAKAIK
                     ADMYENWTDVSGFLVTDPRIVKNPAVIETITYRELRELSYMGATVLHEDAIFPVRKEG
                     IPINIRNTNRPEDKGTFIVESTCRKPKYTITGIAGKKGFCSINIEKSMMNSEIGFGRR
                     VLQVFEDQGISFEHVPSGIDTMTVYVHQDEFEEKEQQVIAGIHRLVQPDFVEMESDLA
                     LIAVVGRGMKSTRGTAGRIFSALAHANVNVKMIDQGSSELNIIIGVENRDFENAVKAI
                     YDIFVLTQI"
BASE COUNT          425 a          376 c          259 g          459 t
ORIGIN      
        1 cctgtaaaat tccatagtca gacgacacac ttcttctggg gtaaaactgg tacgcaccac
       61 atcattggag gctctgttta tacagtattt acagtcaaat acacattcat ttgtataaag
      121 gatcttcaaa agactgatac atctgccatc ctgagcaaaa ctatgacaga ttccggcagc
      181 aatgctgttt cccatgccct ttccatctcc ctttcgatcc acaccactgg atgtgcaagc
      241 cacatcatat ttcgcggcat ccgataaaat ctccagtttc cgcttcaatg tcattgattc
      301 ctgaataatc atcccgtaac aaagctcctt ttatgtaatc tgtgtttcgc attctttatt
      361 attcttaatt tcagaacgtt tgttcttgtt ttattttaga acgcacgttc tgttttgtca
      421 atatcataaa acggcacgaa attttttcta tacaaaaaga gccataaatc aaagattaat
      481 tctaacacta cctttgattt acggctctta tctcattctt ttactattaa agctcccgga
      541 cgacagccag aaatggtaca tgcttacatc agcgaattca tgaataccag acgttctgaa
      601 cgtatgagca gattttcata tatgtgaatg ctgccgacag gtgacctatc cactcaattt
      661 caaatctgtg tcagcacaaa aatatcataa attgctttca ctgcattttc aaaatcacgg
      721 ttttccacac cgataataat attcagctca ctggaaccct ggtcaatcat cttgacattt
      781 acatttgcat gtgcaagggc agagaaaatt cttccggctg taccgcgggt ggatttcatt
      841 ccacgtccta caactgcgat cagggcaagg tcagattcca tttccacaaa atccggctga
      901 accaggcgat ggattcccgc aattacctgc tgctcctttt cttcaaactc atcctggtga
      961 acatatactg tcatggtatc aataccggat ggaacatgct caaaactgat tccctggtct
     1021 tcaaatacct gaagcactct tctgccaaat ccgatttcac tgttcatcat ggacttctca
     1081 atgttgatag aacagaaacc tttctttcct gcaataccgg ttatggtata tttcggcttg
     1141 cggcaggtac tctccacaat aaaagttccc ttatcctccg gacggttggt gttacggata
     1201 ttaatcggaa ttccctcttt tcttacaggg aaaatagcat cttcatgaag aacagtagct
     1261 cccatgtagg aaagctcacg aagttcacgg tatgtgatgg tctcaatcac agccggattc
     1321 tttacaattc tcgggtctgt tacaaggaaa ccggatacat cagtccagtt ttcatacata
     1381 tcagccttaa ttgccttggc aaccagagaa cctgtcacat cagaaccacc tctggaaaaa
     1441 gtggtcacag ttccgtcctc aagaactgca tattttccgc cctcgtggac atagactttc
     1501 tgcccgccga gttcaaact
//
